LOCUS       DANKBV010000001        69377 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.001, whole genome shotgun sequence.
ACCESSION   DANKBV010000001 DANKBV010000000
VERSION     DANKBV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 69377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69377
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.001"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            1..1366
                     /locus_tag="PWY43_000001"
                     /pseudo
     CDS             1..1366
                     /locus_tag="PWY43_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992286.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
     gene            complement(1453..2313)
                     /gene="pdxY"
                     /locus_tag="PWY43_000002"
     CDS             complement(1453..2313)
                     /gene="pdxY"
                     /locus_tag="PWY43_000002"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDL2134932.1"
                     /translation="MKNILSIQSHVVYGYAGNKASTFPMQLLGVDVWALNTVQFSNHT
                     QYGKWGGMVMPKEQIGSIVDGIHNIGELHRCDAVLSGYIGSAEQVAEIIQAFHKVKAE
                     NPNAIYVCDPVMGHPDKGCIVADGVKEGLINLAMAQADIITPNLVELRELTGLTVENF
                     EQAVEAVKVILTKGPKKVLVKHLSRVGKESNKFEMLFANQDGIWHISRPLYQFAKEPV
                     GVGDLTAGLFVANLLNGKSDLEAFEHTANAVNEVMEQTARSGLYELQIIASRDRIVNP
                     QCSYQAVKIA"
     gene            2432..2507
                     /locus_tag="PWY43_000003"
     tRNA            2432..2507
                     /locus_tag="PWY43_000003"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2465..2467,aa:Thr,seq:tgt)
     gene            complement(2602..3555)
                     /gene="accA"
                     /locus_tag="PWY43_000004"
     CDS             complement(2602..3555)
                     /gene="accA"
                     /locus_tag="PWY43_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL2134933.1"
                     /translation="MSQEFLDFELPIAELEAKIESLRAVSEQDDKINLDDEIKRLQKK
                     SEELTKKTFANLDAWQISRMARHPNRPYTLDYIEHIFTEFDELAGDRAFADDKAIVGG
                     IARLDGRPVMVIGHQKGRTVKEKVKRNFGMPAPEGYRKALRLMEMAERFNMPIITFID
                     TPGAYPGIGAEERGQSEAIARNLREMSQLSVPVICTVIGEGGSGGALAIGVGDKVNML
                     QYSTYSVISPEGCASILWKSADKASTAAEVMGLTAPRLKELELIDNIVPEPLGGAHRN
                     VEQIAQNLKQRIVEDLAGLDQLNKEDLLERRYQRLMNYGYV"
     gene            complement(3609..5033)
                     /locus_tag="PWY43_000005"
     CDS             complement(3609..5033)
                     /locus_tag="PWY43_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDL2134934.1"
                     /translation="MKKQITRRQFLQGSALAAGFATVPTPLLAAERPQLNIPPLMDIG
                     RGKPVRLDLRPAQTQFDKGKLVDVWGVNGQYLAPTVRVKSGDFVKLTYLNNLPQKISM
                     NIQGLQASSEMIGTVHRSLDPKSSWSPIVSINQPACTCWYHADTMLNSAFQVYRGLAG
                     LWMIEDEQSKKATLPNKYGVNDIPLILQDQLLNKDGVQVLDKNTSQFFGKRLFVNGQE
                     SPYFNVPRGWVRLRIVNASLSRAYELRLDNEQPLYLIATGLGMLAEPLETESVQLAPS
                     ERVEVLVDLSEGKAVSLISGQKRDIFYKIGQVFADNDELNDNVILELRPEGMAAVLNN
                     KPSLPAFNLEEFNLKISQERRFAIRPADYLINQKRFDPKRIDFTAQKGSIERWYLHST
                     QAVGFTLQGAKFIIETLNRKQYPIKQLAWRDTVWLEAEQEITLLVRFDNTAPEQLPFT
                     FGVSDFMLRDRGTMGQFSVIENGQ"
     gene            complement(5035..5769)
                     /locus_tag="PWY43_000006"
     CDS             complement(5035..5769)
                     /locus_tag="PWY43_000006"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDL2134935.1"
                     /translation="MLKIFRILIVALAAIFISIFGTIYALLRLRHPSSVGVVARWFGA
                     LHKLVGLELITRKKPEINYPVVYIGNHQNNYDMVTISSMVAEKTVSIGKKSLIWIPFF
                     GLVYWATGNIFIHREKRSSAIETMNKAGQIVKERNVSVWMFPEGTRSRGRGLLPFKTG
                     AFHLAISAGVPIVPVVCSNLHNKVDLNRSNNGTVICEMLDPIDTSGYSRENVKELMEK
                     CHQIMLAKFEQLNQEVEQLENTAKNA"
     gene            5916..6413
                     /gene="tpx"
                     /locus_tag="PWY43_000007"
     CDS             5916..6413
                     /gene="tpx"
                     /locus_tag="PWY43_000007"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDL2134936.1"
                     /translation="MSKVTLAGSPIDVAGTFPQKGDVVADFTLVNSDLSDVSLSDFDG
                     KRKVLNIFPSIDTGICATSVRVFNQKAADVANTVVLCISADLPFAQARFCGAEGIENA
                     KVLSSFRHRDLHNKLGVDIISGPLAGLTSRSVIVLDENNKVLHSELVPEIKQEPDYEA
                     ALAVL"
     gene            complement(6498..8123)
                     /gene="yidC"
                     /locus_tag="PWY43_000008"
     CDS             complement(6498..8123)
                     /gene="yidC"
                     /locus_tag="PWY43_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2134937.1"
                     /translation="MNNRGLLLMGLFLVSILIFTQWQQDFDPEIQAQKQAQVAAQTQQ
                     AGDLPSASNANNPIADSITQGKTITVESDVLRLTIDTLGGDVIGSDLLKHNAELHSNT
                     PFKLLQQDGDKIYVAQSGLVGKNGIDTNAGRPQYAVTADSFKLAEGQNEISVPLTFEK
                     EGVVYTKTFTLKRGSYDVGVNFNIKNNTAQAIEVQPYGQLKHSIVESSGNLTMPTYTG
                     GAYSSSETNYKKYSFQDMEKANLSVDTKAGWIAVLQHYFVSAWVPNQDADNSLYSRTN
                     NGVATIGYRGPLTTIAPNSEANLNSKLWTGSKDQKEMGESANHLDLTVDYGWAWFIAK
                     PLFWLLTAIQKIVSNWGLAIIGVTIVVKTILYPLTKAQYTSMAKMRMLQPRITEMRER
                     FGDDRQRMSQEMMKLYREEKVNPMGGCLPILIQMPIFIALYWTFMEAVELRHAPFFGW
                     IQDLSAQDPYYILPLLMGGSMFLLQKMSPTPVADPVQQKVMTFMPVIFTVFFLWFPSG
                     LVLYWLTSNLITIAQQWLIYRNLEKKGLHSRKK"
     gene            complement(8276..8701)
                     /locus_tag="PWY43_000009"
     CDS             complement(8276..8701)
                     /locus_tag="PWY43_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HDL2134938.1"
                     /translation="MTIWKQTATCEQLNTLSQKSAVSHLGIEFTAIGEDWIEAQLQVD
                     ERTQQPFGVLHGGVSAALAETTANAGALMVCEPHQIAVGMELNISHLKSIPAGTKAIA
                     KATPLKLGREVQVWNIDIKDEQGNLCAVARLSTKTLDKR"
     gene            complement(9030..12122)
                     /locus_tag="PWY43_000010"
     CDS             complement(9030..12122)
                     /locus_tag="PWY43_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL2134939.1"
                     /translation="MIPRLSEVPQLDKATQSYLTELVNQHFSGEIASNYADRLSLATD
                     NSVYQQIPQAILFPKSVSDVVILTKLAQKPEYQHLTFTPRGGGTGTNGQSLNNNIIVD
                     LSRHMNQILELNVEERWVRVQAGVVKDQLNQFLKPHGLFFSPELSTSNRATLGGMINT
                     DASGQGSLQYGKTSDHILAIKSVLINGDILETQAVKSADFFAHIEGLNLSALGKTLHR
                     EIFNRCKTLRPKVLNELPQLNRFITGYDLKNVFNHGESEFNLTRILTGSEGSLAFICE
                     AKLNLLPIPKYRTLINIKYNSFDAALRSAPFMLEAKALSVETVDSKVLNLAKQDIVWH
                     SVSDLLTEDPANPILGINIVEYASSDQTQIETQVKALCQSLDQKMANNEAGIIGYQLT
                     SDVNSIEKIYAMRKKAVGLLGNAKGWAKPIAFVEDTAVPPENLADYIVEFRALLDRYN
                     LPYGMFGHVDAGVLHVRPALDLCDKAQVETFKQISDQVVELTAKYGGLIWGEHGKGMR
                     SYYGERFFGETLWQELRYIKTLFDPYNRLNPGKICTPLESETSLYPIDSQMRADFDRQ
                     IPIQVREEFKGAMNCNGNGLCFNFDVHSTMCPSMKVSGNRLYSPKGRATLVREWLRLL
                     TEQNVSPDDLIFKPRKQAVKLADFVAKIRNTLNKNKEYDFSHEVKAAMDTCLACKACA
                     SQCPIKIDVPTFRSQFNELYHSRYLRPLKDYVVSNLEFIAPLMAKQPRFFNFFSTSTF
                     AEKAANKTIGMTYLPALSVPNLQHQLVEVGYQGESLESLEQQATSGDFSAKNCKTLFI
                     VQDPFTSYYDAKVVADFIALIKKLGFKPIVLPFKPSGKAQHVKGFLHKFAKTAKNQAD
                     FLNRVAKLGLPMVAVDPALTLLYREEYKEILKEARGDFKVLLAHEWLREVIKNGELEN
                     CKKSANSDRLPWYLFAHCTESTALPSSMKEWQAIFAHFGETLLNENVGCCGMAGTFGH
                     ETKHVEMSKAIYQQSWQIKLKNKPLERCLATGYSCRSQVKRFEQQGIKHPIQALLDVI
                     "
     gene            12544..13662
                     /gene="ompA"
                     /locus_tag="PWY43_000011"
     CDS             12544..13662
                     /gene="ompA"
                     /locus_tag="PWY43_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119863613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDL2134940.1"
                     /translation="MKKTLVALAVLSAAAVAQAAPQANTFYAGAKAGWASFHDGLTQF
                     DHEGDGGGFDLKYGINRNSVTYGVFGGYQILNQNNFGLATELGYDYFGRVRGNTTFAD
                     DKERTAAKHSAHGAHLSLKPSYEVVPNLDVYGKVGVALVRNDYYVQQRVDKDSRIKAH
                     NLKPSLLLGAGLEYAITPELAARVEYQYLNRVGNVDKAVRKTVSIPTGTNFQYSPDIH
                     SVSAGLSYRFGQGAAPVEAPEVVTKNFAFSSDVLFDFGKSSLKPAAATSLDAAHAEIS
                     NLGLANLAIQVNGYTDRIGKEASNLKLSQRRAETVANYIVSKGTNPANVTAVGYGEAN
                     PVTGHTCDAVKGRKALIACLAPDRRVEIQVQGSKEVTM"
     gene            complement(13728..14174)
                     /gene="matP"
                     /locus_tag="PWY43_000012"
     CDS             complement(13728..14174)
                     /gene="matP"
                     /locus_tag="PWY43_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDL2134941.1"
                     /translation="MRYQKLITQETTWKWQYLLKKHREGENITKYEEQSLIDLKVQFL
                     STLQNSPDEVEKWIKSEMTPEQRKKMRQSVRAKRKRFFNAEKQTTKKKSIDLEYSSWL
                     RLSKYAKSQKLTLSEAIVKLTDELENKQLYLEQVAKMKSSLKDLLK"
     gene            14267..15904
                     /locus_tag="PWY43_000013"
     CDS             14267..15904
                     /locus_tag="PWY43_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2134942.1"
                     /translation="MTLSLLSSQKLVMQNDFSRLDEKLNFLDFQPQAKQALNQFLQSQ
                     FGALLVLKSEIFPEFINEITEYLKKYSVDLPIISKMDFNKNSLFGYSVFLERELKTEY
                     VKGAIHEADKGILIVNIRSLLLDITQWDKLKQALLFGEYEPNSVNHSPYLSAKEKSTF
                     KLILVGEREDISTLSAYDDGLYQFGQYTEIKSYLSLSDNVEQWGGYVQNYAENLINKR
                     LTSTALNQFLQAYIRESESRELVSISPTLLRKHLLGIQNFYPNVTEIDEVKGYFDYLE
                     QQSSILNQYTVGDILTNQLYIETEGEEIGQINGLSVIEFEGIPHSFGEPLRMSCNVQY
                     GDGEITDIERKVELGGNIHSKGIIIAQSCLANLLELPTQLPFSASIAFEQSYSEIDGD
                     SSSLAIFNVLVSALAKVGLPQSIAVTGAIDQFGNVLSVGGVNQKIEGFFNICHARQLT
                     GKQGVIIPAACVSHLSLSSEVVEAVEQGLFKIWTVENVFDAIAILFERDFYDDEKAPN
                     SAQTALFTLIHQNLDERESEETSGSFLSKICKKIKLD"
     gene            16017..16547
                     /gene="fabA"
                     /locus_tag="PWY43_000014"
     CDS             16017..16547
                     /gene="fabA"
                     /locus_tag="PWY43_000014"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDL2134943.1"
                     /translation="MNSCTPIIKPSYTYEELLLSGKGELFGEKGPQLPAPPMLMMDRI
                     IEMNQEKGNFEKGYIEAEFDIKPDLWFFNCHFINDPVMPGCLGLDAMWQLVGFYLGWI
                     GGEGKGRALGVGEVKFTGQILPTAKKVTYRIHMKRVVNRKLVMGVADGEVEVDGRVIY
                     TATDLKVGLFQDTTAF"
     gene            complement(16623..17549)
                     /gene="rfaD"
                     /locus_tag="PWY43_000015"
     CDS             complement(16623..17549)
                     /gene="rfaD"
                     /locus_tag="PWY43_000015"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDL2134944.1"
                     /translation="MIIVTGGAGMIGSNIVKALNEIGRKDILVVDNLKNGEKFINLVD
                     LDIADYCDKEDFIASIIAGDDFGEIDAVFHQGACSATTEWDGKYLMQNNYEYSKELLH
                     FCLDRQIPFFYASSAATYGGRSDNFIEKREFEGPLNAYGYSKFLFDEYVRRILPEAES
                     PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFAGSEQFLRDFVYVEDVA
                     KVNLWAWQNSISGIFNLGTGKAESFQEVANAVIKYHGKGEIETIPFPDHLKSRYQTFT
                     QADLTNLRAAGYTGTFKTVAEGTAEYMAWLNR"
     gene            complement(17643..18155)
                     /gene="def"
                     /locus_tag="PWY43_000016"
     CDS             complement(17643..18155)
                     /gene="def"
                     /locus_tag="PWY43_000016"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2134945.1"
                     /translation="MAILDVVLYPDEKLATVCEPVEQVDEALNRFIDDMFETMYEHEG
                     IGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESSGETGIEEGCLSIPGHRALVP
                     RKEKVKVKALNRQGEEIIIDADDLFAICIQHEIDHLNGVLFVDHISNLKRQRIKEKMQ
                     KLKKQIARAK"
     gene            18244..19389
                     /gene="dprA"
                     /locus_tag="PWY43_000017"
     CDS             18244..19389
                     /gene="dprA"
                     /locus_tag="PWY43_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL2134946.1"
                     /translation="MAYQYDLLRLLQVQIGPQRIGRLLAELDFAEFCAYDKTQLRQIG
                     WNEKQIQRWFNPDKNWIEQALTWAEQPNQQILSLFDKAYPFLLRQISTAPPILFVKGN
                     VESLSLPQIAIVGSRDYSPYGEYWAGQFAADLVKHNMAVTSGLAIGIDGFAHKKVVEY
                     QGITIAVLGSGLNRIYPARHQKLAEQIVESGGALVSEFSPNQPPIAENFPRRNRIISG
                     LSLGTLVIEATINSGSLITARYALEQGREVFALPNAVQNPYAQGCHKLIKEGALLVEG
                     VEDILEAVASQYQPQQIPLFDEPKPIVSTSLQAVKSSEKFAKNPPNLTACQQQIYQHI
                     SLEPIAIDDLARATELTIETLLVELLGLELAGVIKQVSGGYVMDNGY"
     gene            complement(19484..20839)
                     /locus_tag="PWY43_000018"
     CDS             complement(19484..20839)
                     /locus_tag="PWY43_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2134947.1"
                     /translation="MESLLFFILDILKVPSVLVGLIALVGLVAQKKSVSDIVKGTIKT
                     ILGFLVLSGGATVLLSSLTPLGGMFEHAFNVQGIIPNNEAIVSMAIEKYGTATALIMA
                     FGMVANILIARFTRLKFIFLTGHHTFYMACMIGVILTVAGFEGIQLVFVGALTLGLIM
                     AFFPTIAHFYMKKVTGSNDVALGHFGTLGYVLAGAIGQLVGKGSKSTEEMDLPKNLGF
                     LRDSSISISLTMMAIYYILAIASGSEYVSTLSGGQHYLVYATIQAITFAAGVYVILQG
                     VRLILAEIVPAFTGFSEKLVPDAKPALDCPIVFPYAPNAVLVGFLASFLGGILSLAIL
                     GQLNWVLILPGVVPHFFCGATAGVFGNATGGRRGAIIGAFIHGILITFLPVFLLPVLG
                     SLGFANTTFSDSDFGGIGIVLGYMAQYFDKNIITLIIIGLFTLLVAYNYLAKKPAEPK
                     A"
     gene            complement(20852..21121)
                     /locus_tag="PWY43_000019"
     CDS             complement(20852..21121)
                     /locus_tag="PWY43_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2134948.1"
                     /translation="MKIMAVCGSGLGSSFMMEMNIKKVLAKLGVEAEVSHTDLSSVVA
                     DDADLFVMAKDIAASSNVPKEKLIVVKNIVGLAEFEEKLTAYFNQ"
     gene            21352..22374
                     /locus_tag="PWY43_000020"
     CDS             21352..22374
                     /locus_tag="PWY43_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2134949.1"
                     /translation="MANGTSRNRRSSGRVTLADVAKVVGVGQMTVSRALRTPEMVSEP
                     LRKRIEEAIIELGYIPHFSARQLASAESHNLVIVTTSISSTENTLILSALRKSVADSA
                     LQMVILIADQESWFQELINHAPQAIVFLNLDCPPNVREWIKNSNIPTVEIGAIKKEPI
                     HLSVGIDSHEAMEKMIHFLVKKGYREIGLLSAKQDLSIFKQYLESWHTTLFAHHISPL
                     KIHHVVEDISFSAGATLLTEALLNWEAIDALVFLSDELACGALYEALRRHMSIPKDLA
                     IAGLGGLEVGSISYPKLTTIAIPYEQMGKIAGKQLLTLLSGKTLSAEEMIVKLPTKLV
                     IRDSTA"
     gene            22487..22900
                     /gene="secE"
                     /locus_tag="PWY43_000021"
     CDS             22487..22900
                     /gene="secE"
                     /locus_tag="PWY43_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL2134950.1"
                     /translation="MATEIKTKTPEQVEQKGGKSKGANTALWLVAIVFLAVAAVGNAY
                     FSSHFSLIVRVLLLVVLLVGAVALAAITNQGQKAIHFIKESRTELRKIIWPTRPEATQ
                     TTLIVLAMCVVVSLVLWGIDSIIVALITFVTSLRF"
     gene            22902..23465
                     /gene="nusG"
                     /locus_tag="PWY43_000022"
     CDS             22902..23465
                     /gene="nusG"
                     /locus_tag="PWY43_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL2134951.1"
                     /translation="MSEVEVKENGSKRWYVLQAFSGFENRVAVTLREYIKLHKMEEQF
                     GEVLVPTEEVVENVGGKRRKTERKFFPGYVLVEMEMNDDTWHLVKSVPRVMGFIGGTA
                     DRPAPITKREADRILNRVQETAEKPRHRKEFQPGESILVTEGPFADFSGTVEEVDYEK
                     GRLKVSVSIFGRATPVELEFSQVEKQS"
     gene            23698..24126
                     /gene="rplK"
                     /locus_tag="PWY43_000023"
     CDS             23698..24126
                     /gene="rplK"
                     /locus_tag="PWY43_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL2134952.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     RTESLEKGLPIPVVITVYADKSFTFVTKTPPAAVLLKKAVGIKSGSGKPNKDKVGTVT
                     QEQIRQIAETKAADMTGATIETKMKSIAGTARSMGLIVEE"
     gene            24131..24820
                     /gene="rplA"
                     /locus_tag="PWY43_000024"
     CDS             24131..24820
                     /gene="rplA"
                     /locus_tag="PWY43_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL2134953.1"
                     /translation="MAKLTKKMKAIKAGVDSTKAYDINEAVEVLKKFATAKFVESVDV
                     AVNLGIDSRKSDQNVRGATVLPHGTGRTARVAVFTQGANAEAAKAAGADLVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKSGQ
                     IRYRNDKNGIIHTTIGKADFSAEQLKDNLNALLAALTKAKPTTAKGIFIKKVSISTTM
                     GAGVAVDQASL"
     gene            24882..25301
                     /locus_tag="PWY43_000025"
     CDS             24882..25301
                     /locus_tag="PWY43_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2134954.1"
                     /translation="MLRLLSFCFIFLLSACTVLPQIKQLPPQVESVKSFKVENNGQQS
                     LLLIQFYENKWRWIQTDPLGAPIARVWLTKKGWEPDGFVMPNKQAQLLFTALVNALNP
                     HSPPFYLDKGWKIEREKSQFTIWLPDSSKWKVEILGE"
     gene            25388..26611
                     /locus_tag="PWY43_000026"
     CDS             25388..26611
                     /locus_tag="PWY43_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL2134955.1"
                     /translation="MMTLYLSEPAITSPIGYGIVEHLNVLINAKVSPLDTTSQPYLRH
                     NISNSSPKFLGQVSARLRDFPVELSIEHRSRNNQLLWHALAEIEPKITACIEQFGRER
                     IAVIIGTSTTGADENIPVFKAGVQGDYATEQFNQQQILFSAPSDFVAEVYGLTGLSYG
                     ISTACTSGARALISAARLLKAGLCDAVICGGVDTLSPLTISGFSSLEVLSNQQTNPFS
                     ANRNGINIGEGAAVFVMSREPLDEHRIQLLGYGSSSDAYHMSSPHPEGEGAISAFQNA
                     LHNADLDAADIGWINLHGTGTLHNDQMESLAVAKIFGNQTACTTTKPYTGHTLGAAGA
                     VEAAILWGMLCRQHNPTGKLPAQLWDKQADENLPNIAITYEQSRWAAGRRIGASSSFA
                     FGGNNSVLILGESDA"
     gene            26604..27044
                     /locus_tag="PWY43_000027"
     CDS             26604..27044
                     /locus_tag="PWY43_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HDL2134956.1"
                     /translation="MHKFTFPIEQIAPLVPHSGNMILLDRITAFGEDFLTAETNIRPD
                     NPLIKHGKLATYASIEIMAQGVAAWAGCIATQAGEPVRLGYLLGTRKLHLHRQEIPIG
                     SQLQIQIKMSIQDATGFGVFDCQLIDLADNQVLLEGALNVFSPK"
     gene            27134..28567
                     /locus_tag="PWY43_000028"
     CDS             27134..28567
                     /locus_tag="PWY43_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL2134957.1"
                     /translation="MQEAYDIQHLCTRKEDKSFDRKSARKEPKEILRHLIGFANADGG
                     LLVIGIEDNGEITGFNYQKAHQPEEFIHALHNLQKMPIPVTYQQIEVVNSRGLADFVL
                     LFEVTASSGRAITSHDDRVFLRSKDQTLELNFEQRLQLEYEKGQRAFEEQPIARADFT
                     DLDMDLLEQYRQKLHTDRSIEEILKARDLINKEGQITNACILLFGNNPTRYFPNARLR
                     FLRYEGKAPLTGRTFNVVKEFMLEEPIPKLLESAKAVLKSQLREFQRLDENGQFTKID
                     EYPEFAWLEGIVNAIAHRSYSNQGDCIRISMFDDRLEIFSPGGLPRPVTLENMYYTRF
                     SRNPKIARVLYEFGWVKELNEGVNRMAEEMNAYQLNTPSYFEPNENAVRLILENNIEH
                     RTLRALERIEEIFTQDIMATLNKNEVTILQYLYENEMITVKIASEILDRSKELSRRIL
                     KSLEQKNILTWHGLSPKDPTQYYTLKK"
     gene            28581..29309
                     /gene="fabG"
                     /locus_tag="PWY43_000029"
     CDS             28581..29309
                     /gene="fabG"
                     /locus_tag="PWY43_000029"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL2134958.1"
                     /translation="MNNTVLITGSSRGIGKAIALTLAQQGFDIVVHCRSRVEEAEAVA
                     QAVRNLGKNARVLQFDVADREKVRSILEADVEQHGAYYGVVLNAGLTRDNAFPALTDE
                     DWDSVLRTNLDGFYNVLHPIMMPMIRRRKAGRIVCITSVSGLIGNRGQVNYSASKAGI
                     IGAAKALAIELAKRKITVNCVAPGLIDTDILDENVPIEEILKMIPAGRMGDPDEVAHA
                     VAFLMDEKAAYITRQVIAVNGGLC"
     gene            29372..30517
                     /locus_tag="PWY43_000030"
     CDS             29372..30517
                     /locus_tag="PWY43_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL2134959.1"
                     /translation="MKRVVITGIGAVTAFGKEWAEIHGYQPPKHWNRKQLRSLGRVSQ
                     FVVEASERALAQAGLLDENGVIFDWLKDGRMGVACGSSTGSTNDIKDAAELLMTGKSD
                     GFNANTYVRMMPHTTAANIGIFFGLTGRIIPTSSACSSGSQGIGYAYEAIKYGMIPMM
                     LAGGGEEFCASEVYVFDSLYAASRRNDNPKGTPRPYDKDRDGLVIGEGAGMFVLEELE
                     HALARGANIIAEVVGYGANSDGSHVTRPQATTMQRCMELALKDAGIQPNQVGYVNGHG
                     TATEQGDIAETQATTAVFGKIPLSSQKSYLGHTLGACGALESWFSIEMMRDGWFAPTL
                     NLENVDERCGDLDYLQGDGREIHTDYVMNNNFAFGGMNTSLIFKRWK"
     gene            30531..31436
                     /locus_tag="PWY43_000031"
     CDS             30531..31436
                     /locus_tag="PWY43_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
                     /protein_id="HDL2134960.1"
                     /translation="MKKILLVYYSQTGQLTRLAENFVQPLEQAGVFVEKLAIKPQQEY
                     PFPWRFMRFFNTFPETVHLTPPPIEPLPFQHEIYDLVIIAYSVWFLSPSQPITAFLQS
                     EQAKKVLKDTPVITLIGCRNMWLMAQEKVKKLLAESEAKLIGNVVKVDQSNDWASFIT
                     TPLWMLTGKKKIGKLPSAGIAESEMEDVKRFGAKLLHIFSQNQPLDSTLFRQMQAVKI
                     DEKLMMSEKVGARSFHLWGKLLIKCGQISPHLRQAVLYFYILFLVTLILTVVPISTVI
                     KRLLRPLLKEKLEKQKRYFAEPSGE"
     gene            31459..32601
                     /locus_tag="PWY43_000032"
     CDS             31459..32601
                     /locus_tag="PWY43_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDL2134961.1"
                     /translation="MLSNVYITNTSAFLPNAPVSNDAMEAVLGMVGETPSRVRKMILR
                     SNGIQYRYYAIDPITRQATHSSTELAVQAVKKLGNSATSATCLAVGTSYPDQIMPGQG
                     VMVHGLLDAEPMEVLTTAGVCVAGMAAMKHAYNAVRIGEHRAAIATASETASAIMRSE
                     HFRAEIDHKKLDDARPEIGFEKDFLRWMLSDGAGAVLLANQPNEKGLSLKIHWIDLVS
                     YANEMPVCMYAGAEFIDGNYTGWKAMSEAQRAECSAMAVKQDVKLLNENIVKYTVEKA
                     LERIIPKYKLNAEEIDYFLPHYSSGSFRDKLLEGLQRINFLIPQEKWFTNLTTKGNTG
                     SASIYIMLDEFIRTHDLKQGQKILCYVPESGRFSSCFMLLEAVVNS"
     gene            32650..32865
                     /locus_tag="PWY43_000033"
     CDS             32650..32865
                     /locus_tag="PWY43_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2134962.1"
                     /translation="MDINDIPQDNSKIFNGERKVIYARNPQPEIRQMISSILACYAWD
                     ENNPFVAKSDARLKALAEVVGKAEIGD"
     gene            33146..33637
                     /gene="rplJ"
                     /locus_tag="PWY43_000034"
     CDS             33146..33637
                     /gene="rplJ"
                     /locus_tag="PWY43_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL2134963.1"
                     /translation="MALNLQDKQAIVAEVNEAAKGALSAVVADSRGVTVEKMTELRKA
                     AREAGVTMQVVRNTLLRRAVEGTEFECLTDTFTGPTLIAFSNEHPGAAARLFTEFAKT
                     NKEFELKGAAFEGKKQDVAFLATLPTYEEAIARLMGTMKEAAAGKLVRTLAALRDQME
                     AAA"
     gene            33696..34064
                     /gene="rplL"
                     /locus_tag="PWY43_000035"
     CDS             33696..34064
                     /gene="rplL"
                     /locus_tag="PWY43_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL2134964.1"
                     /translation="MSLTNEQIIEAIASKSVSEIVELITAMEEKFGVSAAAAVAAAPA
                     AGAAAAEEKTEFDVVLAEAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGISK
                     AEAEALKKELEEAGAKVEIK"
     gene            complement(34171..35001)
                     /locus_tag="PWY43_000036"
     CDS             complement(34171..35001)
                     /locus_tag="PWY43_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2134965.1"
                     /translation="MVQKPLNHYLEMQEHFLYVPYLHHHRRIRVLLPKDYHNEGWERY
                     PVLYMHDGQNVFYSKESYSGHSWKIIPTIKSHQELPKMIIVGIDNAGANRLNEYGPWK
                     TTTGSTPETRNAGGLGEEYAKWVVETVKPFIDIHYRTKPQQEHTLLAGSSMGGIITAY
                     MGSRYPHIFGHLGVFSLASWFSEPDFLRFTHQYPLQPNTKVFIQVGTNEGDEIDSHFI
                     SNTNQTYIDCSLNYYQALIRIGVPLDNIRLRIMANEIHHEMHWADHFVEFLHFSLLRK
                     "
     gene            35227..36768
                     /locus_tag="PWY43_000037"
     CDS             35227..36768
                     /locus_tag="PWY43_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDL2134966.1"
                     /translation="MQASLQDILEVVKAKNLTYQQKLMSLGNIAERLFNPIELLGYTE
                     EEWEHIENQMICDLNEGYAIYRPRYILPDYNVYMQKGCQFLDLPPPTNLDEALDGLLI
                     LYSHVPSITTFPVYIGRLDQLLEPFITDEEQDYIKIKRFLNHIDKTISDSFCHANIGP
                     NDTKAGRLILKAVIELENTTPNMTIRYDKTKTSREFAELAAKACLLVSKPSFANDAYY
                     IQDLGEEYGIASCYNALPECGGAYTLTRLRLGTIGRACASVEQMVNEMLPKVAKLALS
                     TMDKRHKFLVEESNFFESSFLVQEGFIKRTNFTSMIAIVGLADAVNHLLKQEGLNETF
                     GQSKRGDEIATLIMDKLQAVNDAHKGLYVERTNNQYLLHAQVGANNHAEDKMNTPAHR
                     IRVGEEPTLLAHLKQSAPFHKYFPSGTGDLFAFDQTYTDHLDAVVDIIDGSFAQGYRY
                     ITTYLKNTDLIRVTGYLVKKSEVERYRKGEAVLRDTTWYGSGTDDCAKVFDRQLRDEE
                     NVNAG"
     gene            36771..37559
                     /locus_tag="PWY43_000038"
     CDS             36771..37559
                     /locus_tag="PWY43_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HDL2134967.1"
                     /translation="MAALSEIFVPLHRIIPFSNVEGQGNRTSIFLQGCKLNCLYCHNP
                     ETIPRYSKEAKQVSLQYLYDQVMEAVPFIRGVTISGGEPTIHHKKLVPLFEALREQGL
                     TCYLDSSGFFDFEATEPLINVTDKFLFDLKGDGAGLQTLCFDRKNQAGKVPEQALPNV
                     NHIKPENLARNLQNLAKLLPLDKVEEVRLVYLNEFYNAHHLLEKVADLLKPYPDVLLK
                     IIRVHTKGARDETGLARFVPTIEQTDELAAFAKRHGIQKVITIY"
     gene            37572..38829
                     /locus_tag="PWY43_000039"
                     /pseudo
     CDS             37572..38829
                     /locus_tag="PWY43_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(38908..40917)
                     /locus_tag="PWY43_000040"
     CDS             complement(38908..40917)
                     /locus_tag="PWY43_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL2134968.1"
                     /translation="MSAENMPSVIRFEQAVAKKDYESACTELLSILSKLDSNFGGISN
                     IELNMPEQIENLENDKAIYFCTRMAVAITRLFEDPALEISEHGAMRFLTLQRWIALIF
                     ASSPYVNADHILRTYNRNKESANPNTVDLDATLQALIKFCILYLPESNILLNLDAAWN
                     ASSDLTASLCFALQSPRFIGTSSAFAKRAAILQWFPEKLAQIENLNKLPSAISHDVYM
                     HCSYDIEANKHNVKRSLNAVIRRHLLSVGWEDRKIEQLGTRNNKPVMVVLLEHFHSSH
                     SIYRTHSTSMVAAREHFHLIGLGSDAVDEMGQQVFDEFHLLPQDGSLFDRLSFLKDIC
                     DKNNPAVFYMPSIGMDLTTIFASNTRLAPIQAVALGHPATTHSDFIEYVIVEDDYVGS
                     ESCFSEQLLRLPKDALPYVPSALAPQNVVYNLRENPEVIHIGIASTTMKLNPYFLEAL
                     KAIRDRAKVKTHFHFALGQSSGITHPYVERFIKSYLGNDATAHPHSPYDEYLNILHNC
                     DMMLNPFPFGNTNGIIDMVTLGLVGVCKTGPEVHEHIDEGLFKRLGLPNWLITQTAEE
                     YVTQAIRLAENHEERLAIRRDIIENNKLQTLFSGDPRPMGQIFLAKVQAWLADKNPKN
                     AEVEVKTKKVRKAATASQSAKKQTTSKTQTAKAEKDNAAKTETKS"
     gene            complement(40927..42264)
                     /gene="rimO"
                     /locus_tag="PWY43_000041"
     CDS             complement(40927..42264)
                     /gene="rimO"
                     /locus_tag="PWY43_000041"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDL2134969.1"
                     /translation="MSASPNIGFISLGCPKNLVDSERILTELRADGYNIIPSYENADL
                     VIVNTCGFIDSAVQESLESIGEALEANGKVIVTGCLGAKENQIREVHPKVLEITGPHS
                     YEAVMNHVHKYVPKPEFNPYTSLVPKQGIKLTPKHYAYLKISEGCDHRCTFCIIPSMR
                     GDLASRSIVQVLDEAKRLVDAGVKELLIVSQDTSAYSLDRKKEEETKTVFWNGMPIKS
                     NLITLCEQLGSMGVWVRLHYVYPYPHVDDLIPLMAAGKILPYLDIPLQHASPKILKAM
                     KRPGSIERTLERIKKWREICPELTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGC
                     FKFSPVEGAVATDMPDQVPEEVKEERFHRFMQLQQEISAARLQAKIGKTVQVIIDDID
                     EEGIIGRSMADAPEIDGVVYVDNPSNQLVTVGQIISVTITHADEYDLWGILNN"
     gene            complement(42419..43111)
                     /gene="mtnN"
                     /locus_tag="PWY43_000042"
     CDS             complement(42419..43111)
                     /gene="mtnN"
                     /locus_tag="PWY43_000042"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL2134970.1"
                     /translation="MKIGIIGAMAQEIEILRQVMVEPKVTELAGCKIFEGKINNTRVA
                     LLQSGIGKVAAAIGTTLLIQLTQPDMIINTGSAGGLDPDLNVGDILISTEVRYHDVDV
                     TAFGYEIGQLPANPPTFSPNEQLVELAQKEAKKAGYNVVSGLICSGDAFINGNDKINQ
                     IRQNFPNVVAVEMEAAAIAQVCHGFNLPFVIVRAVSDVADKESHLSFEEFLPLAAEKS
                     STIVLAMLNNID"
     gene            complement(43169..43390)
                     /locus_tag="PWY43_000043"
     CDS             complement(43169..43390)
                     /locus_tag="PWY43_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HDL2134971.1"
                     /translation="MDNNYTLSAKITELETKVAFQELIIEELNQALIEQQFAIDKLQT
                     QIRHIAEKLKGAQLSQVASQAEETPPPHY"
     gene            43478..44203
                     /gene="fkpA"
                     /locus_tag="PWY43_000044"
     CDS             43478..44203
                     /gene="fkpA"
                     /locus_tag="PWY43_000044"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2134972.1"
                     /translation="MLKTKLSLFTLIVGTLFSTNVMAEEKADPKFVDDSSYAVGVLMG
                     KNINGVIESQKDIFTYNQDKILAGVQDTLKKTGKLTDEDLQKQLKSLDAYLSAKEAKI
                     NEEKTKATVAAGDKFRADYKKQSGVKETASGLLYKIEKAGEGNSPKAEDTVKVHYKGT
                     LTDGTVFDSSYDRGEPIEFQLNQLIPGWIEAIPMLKKGGKMEIVVPPALAYGDRAAGK
                     IPANSTLKFEIELLDFKAADAKK"
     gene            44300..44956
                     /locus_tag="PWY43_000045"
     CDS             44300..44956
                     /locus_tag="PWY43_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2134973.1"
                     /translation="MTKKFTPFSDEDHAILASYFPMVDGIAALIGEHCEIVLHSLEFL
                     EHSAIYIANGHNTNRKIGSPITDRALKSLHHMATDSVSKPYFTKAKGGVLMKSITIAI
                     RNGKQHVIGLLCININLEVPTSQFLNSFIAPVDTASEVTFASSVEDLVSQTIQTTIDD
                     VMADRQISNNNKNRQIVISLFEKGIFDIKDSINQVAEKLDISRHTVYLYIRQIKQDND
                     "
     gene            44956..45333
                     /gene="tusD"
                     /locus_tag="PWY43_000046"
     CDS             44956..45333
                     /gene="tusD"
                     /locus_tag="PWY43_000046"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDL2134974.1"
                     /translation="MDYVLAIKSPVYGSQGAFLAYQVAEELLKANHTICQIFFFQEGV
                     SNANALVNPASDEVNLVEMWKKLAKLHRLPLHLCIAAAQRRGVVDEQISANATHNLAE
                     GFTLAGLGEFSQAVLKADRLLTF"
     gene            45335..45706
                     /gene="tusC"
                     /locus_tag="PWY43_000047"
     CDS             45335..45706
                     /gene="tusC"
                     /locus_tag="PWY43_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDL2134975.1"
                     /translation="MKKYKLAFLFTQPPFGSAVSREGLDALLAASAFCDEQEIAILFI
                     NDGVFNLIADQQPEKLLQKDHISTFKLLELYDLTECFICQESVSLRGLDNADWLLRML
                     QFKTHAEMIALLNQAEKVMTF"
     gene            45716..45991
                     /gene="tusB"
                     /locus_tag="PWY43_000048"
     CDS             45716..45991
                     /gene="tusB"
                     /locus_tag="PWY43_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDL2134976.1"
                     /translation="MLYTLSKANYDEETLNFFFSHLSENDAVVLWQDGVLQAVKSPQF
                     FAKLPRCYLLAQDVQARGLQEKLTAFQCISLSELVKLTAVYFPQVAL"
     gene            46107..47006
                     /locus_tag="PWY43_000049"
     CDS             46107..47006
                     /locus_tag="PWY43_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2134977.1"
                     /translation="MVMAEENNTWVDKKHETVSTTLDRWANNINSWLGDVDPDKPGTA
                     SLRVMLDSRWDKHDKFTYKPRVRGKIKLPILKKHLNVVFGDDELDNELTDKNHAGKLY
                     KNVNPNKRYNSREARDSNSSIGLRWSDGIKALGIETDLDAGIRSGSDIFGRLRISKDW
                     KITDQFSTRLEQIYRYGSESKHYLRTNFENKYTETDTTFIMNHTNFEYRHDVDEERSW
                     GNSLYRQHNFKPLTHISYGISMGGRLDKELSKFNYWGPFVSYRQPIWRNWLFIQPEIS
                     FYNDKDKDRKHFIQTFVRLEAIF"
     gene            47356..51381
                     /gene="rpoB"
                     /locus_tag="PWY43_000050"
     CDS             47356..51381
                     /gene="rpoB"
                     /locus_tag="PWY43_000050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2134978.1"
                     /translation="MAYSYSEKKRIRKSFGKRPQVLNVPYLLTIQLDSFEKFIEKDPE
                     GQQGLEAAFRSVFPIVSNNGATELQYVSYELGEPVFDVRECQIRGTTYAAPLRVKLRL
                     VSYDREAAAGTVKDIKEQNVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFARIDRRRKLPATIILRALGYTTE
                     EILNLFFEKIKFEIADNKLLMTLVPERLRGETASFDIAANGKVYVEMGRRITARHIRA
                     LEKDNVTQIEVPVEYIVGKVAAQDYVDLSSGEVICPANMEISMEMLANLSQAGYNQIE
                     VLFTNDLDHGPYISETLRVDPTYDRLSALVEIYRMMRPGEPPTKEAAEALFDNMFFSA
                     ERYDLSAVGRMKFNRSLEIPEGVGSGILSNDDIIGVMKKLIEIRNGRGEVDDIDHLGN
                     RRIRSVGEMAENQFRIGLVRVERAVRERLSLGDLDGVTPQDLINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHPTHYGRLCPIETPEG
                     PNIGLINSLSVYARTNNYGFLETPFRKVVNGQVTEEIEYLSAIEESNNIIAQANSNLD
                     ENFRFTDTYVTCRQQGESGLFKPEEVQYMDISTQQVVSVAAALIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMEKPIALDSGVAIVAKRGGTVQYVDASRIVVKVNEDE
                     TIPGEAGIDIYNLIKYTRSNQNTCINQVPCVNLGEPVARGEILADGPSTDLGELALGQ
                     NIRVAFMPWNGYNFEDSMLVSERVVQEDRFTTIHIQELSCVARDTKLGAEEITADIPN
                     VGESALSKLDESGIVYIGAEVKGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGTSGTVIDVQVFTRDGVEKDKRAKEIEEMQLCEAKKDLAEELEILEAGLF
                     TRVRNLLIEGGVKESALDGVAREKWLEQTLEDEAKQNQLEQLAEQHEELRKEFERKLE
                     IKRNKIIQGDDLAPGVLKVVKVYLAVKRQIQPGDKMAGRHGNKGVISKINPVEDMPYD
                     ENGQPVEIVLNPLGVPSRMNIGQILETHLGLAARGIGDQIDKMIKQQQEVAKLREYMQ
                     KAYDLGHGSQVVDLSTFSDEEVMRLAQNLRKGLPLATPVFDGAHESEIKGLLELGGLP
                     TSGQITLYDGRTGEKFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGTHYMEPGIPE
                     SFNVITKEIRALAIDMELDEA"
     gene            51644..55927
                     /gene="rpoC"
                     /locus_tag="PWY43_000051"
     CDS             51644..55927
                     /gene="rpoC"
                     /locus_tag="PWY43_000051"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2134979.1"
                     /translation="MKDLVKFLKAQTKSNEDFDVIKIGLASPDKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRER
                     MGHIELACPVAHIWFLKSLPSRIGLILDMPLRDIERVLYFESYVVTEPGMTDLEKNQL
                     LTEEQFMDAEERWGDEFEAKMGAEGIQALLRDLDLEHQCETLREELQETNSETKRKKI
                     TKRLKTLEAFLQSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLDLVAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSNKRPLKSLADMIKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFRPFIYSKLENRGIA
                     STIKAAKKMVEREDPIVWDILAEVIREHPILLNRAPTLHRLGIQAFEPLLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLTLEAQLEARALMMSTNNVLSPASGDPIIVPSQDVV
                     LGLYYMTREKVNGKGEGMYFLDPREAEKAYRTGQAELHSRVKVRVTEYEKNEAGELEA
                     KTSLVDTTIGRSILWMIAPKGMPFSLFNQTLGKKAISKLINESYRRLGLKESVVLADQ
                     IMYTGFAYAARSGASVGIDDMVIPEQKYAIIEAAEAEVAEIQEQFNGGLVTAGERYNK
                     VIDIWAAANERVAKAMMENLSTEEVINREGNPEKQASFNSIFMMADSGARGSAAQIRQ
                     LAGMRGLMARPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLT
                     RRLVDVAQDLVIIEDDCGTHEGIVMTPLIEGGDVKEALRDRVLGRVVAEDVLKPGTDE
                     VLIPRNTLLDEKWCDVVDRESVDVIKVRSVVTCDTDFGVCAKCYGRDLARGHLINQGE
                     AVGVIAAQSIGEPGTQLTMRTFHIGGAASAAAKESSIQVKNAGTIKLTNAKFVTNKEG
                     KIVLTSRNTELTVIDTFGRTKENYKVPYGAVLSKHDGAEVAVGEVVANWDPHTMPVIS
                     EVSGRIQFSDIVDGLTVTRQTDELTGLSSIVVQDVGERATAGKDLRPALRLVDSNGKD
                     ILIPGTDVAAQYFLPGKAIVTLDDGAEIEVGEALARIPQESVGTKDITGGLPRVADLF
                     EARKPKEPAILAEISGIVSFGKETKGKRRLVITPAEGEAFEEMIPKWRQLNVFEGEMV
                     QRGDVISDGAETPHDILRLRGVHAVTDYIVNEVQEVYRLQGVKINDKHIEVIVRQMLR
                     KAVITNAYDSEFLEGEQVEVARVKIANRKRAEEGKPLVEFERELLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKQDELRGLKENVIVGRLIPAGTGFAYHQARAKKRAQLEA
                     GIAVVPEFEAPAAKANSFVTDADIEAEFEFVADDATQSLAALLNAGEEE"
     gene            56322..57620
                     /locus_tag="PWY43_000052"
     CDS             56322..57620
                     /locus_tag="PWY43_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
                     /protein_id="HDL2134980.1"
                     /translation="MDISWYKPRNYLHFDSPITLQQAIKLVANPKHSFYPFLSYTLSV
                     AKIKKDKNEGKLVRLIKNRGISYSSHKDSHIFSYYAYLLNEKYENLLKNKYYILDDSI
                     LAFRKLNKSNIDFARIAFDSIRSAQNCTVLGLDISKFFDHIDHKILKDMWCKVLGVPS
                     LPEDHHSVFKAITKFVKVDRDTVFEFFNISLHNPRKNGKNRICSPREFREYRKLKKPD
                     LFNDNPAFFINKGQKGIPQGSPISALLSNIYMLEFDVLVKEKIMECKGSYFRYCDDIL
                     CIIPNEYEEFILDYITGEIKSKLKLEINKDKTEVVKFQYCNRTKKIINEKKLQYLGFI
                     LHNNSISIRSSAFTRYSNKMKRGVSLAKQTQGKYNRIRIRRGVAIKGIYKRKLFSKYS
                     HFGKSNFISYGKRAYSSMDSKVIKNQLKPLWYRLKKEIYL"
     gene            complement(57872..59152)
                     /gene="hemL"
                     /locus_tag="PWY43_000053"
     CDS             complement(57872..59152)
                     /gene="hemL"
                     /locus_tag="PWY43_000053"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL2134981.1"
                     /translation="MSISEQLFEQAQKVIPGGVNSPVRAFKGVGGTPVFIEKAEGAYI
                     TDSDGKQYIDYVGSWGPMVLGHNHPAIIDAVLKAVPKGLSFGAPTAAEITLAELVCKL
                     VPSIEMVRMVSSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHSDSLLVKAGSGALTL
                     GQPSGPGVPADFAKHTLTCTYNDLDSVKAAFEQYPNEIACLIVEPVAGNMNCIPPQEG
                     FLQGLRDLCTQYGAVFIIDEVMTGFRVALGGAQSYYGVTPDLTTLGKIIGGGMPVGAF
                     GGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGNETILAEKTKTLAEG
                     FKALADKHNVPLTVQYVGGMFGLFFTEQKAVNNFQEVMKCDAAKFNRFFHLMLEQGVY
                     LAPSAFEAGFMSLAHSDEDIARTLEAADKAFAQL"
     gene            59294..60358
                     /gene="wecA"
                     /locus_tag="PWY43_000054"
     CDS             59294..60358
                     /gene="wecA"
                     /locus_tag="PWY43_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDL2134982.1"
                     /translation="MWLTFLTVFIVSFLALIVMRPVAEFVGLVDKPNFRKRHQGLIPL
                     IGGIALFIGNLTFYFMQWQDMRLPELYLTAVTILLIIGVLDDRFDISPLLRAGIQAAL
                     AGAMIYSGLSLSSLGQIIAPFSIELGWLGIVFTVFITIGVINAFNMVDGIDGLLAGLS
                     SVSFAGLGTLMFINGEPSLGYWCFALIFVLLPYAMFNLSLFGAKWKVFMGDSGSMLIG
                     FTIIWILLLSTQGQGSPISPITGLWLIAVPLIDMVAVIIRRLKKGKSPFKPDRLHLHH
                     LMMRAGLTSRQALLVITLWAGFCSTIGVLGEVYYWNQWVMMLMFIGLFFLYAYSITHA
                     WRVTRFVRRLKRRAKRSKQL"
     gene            60369..61181
                     /locus_tag="PWY43_000055"
     CDS             60369..61181
                     /locus_tag="PWY43_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS chain length-determining protein"
                     /protein_id="HDL2134983.1"
                     /translation="MKTLFYRLLVLILFAAIGAGAGYGLSYTQPEKWKVTAQFEQPTI
                     PDLGNYYSLLSTYHFLNSNSQEANSQTAQQQASEQVYAEFKRNLTSADLLQAYLLQTE
                     AVKLKAQVAKKSPAVVAAELVEQFRFEKDMATSADNVSLIAENAEEAQKLLADFIPLV
                     NTQARDKLNADLIAKWKVLFQQIKTASELNINGKQGSQDWAAKLQMMRSVQPLDNQLT
                     AYRLVKSPSVPLKSESPDPPILADDWGIKWLTAGLYAQFCGESESSTCGTLI"
     gene            61222..62469
                     /gene="wecC"
                     /locus_tag="PWY43_000056"
     CDS             61222..62469
                     /gene="wecC"
                     /locus_tag="PWY43_000056"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL2134984.1"
                     /translation="MMSKTEFNTICILGLGYIGLPTSVVFANAGKQVIGVDINPQVVA
                     SLNQGNIHIIEPDLQTAFTQAVKKGNFFATAKPQPADAFIIAVPTPLCGKQPDLTYIQ
                     NAAAMIAPCLRKGNLVVLESTSPVGTTENLAEWLSQARPDLAFPADVSIAYCPERVLP
                     GNIMTELVENDRIIGGLSEQCSQQAVDLYQIFAKGRCVTTTARTAEMCKLVENSFRDV
                     NIAFANEISMICDALKINVWELIELANLHPRVNILQPGAGVGGHCLAVDPWFIVSTSP
                     QQARLIRTAREVNDSKPQWVIEQVKTSVADCAMEQDCKPSELTIACLGLAFKPNIDDL
                     RESPALAITRQLAAWHQGSLLAVEPNIQQLAGEKITLVDLNTALSQADILVLLVDHTS
                     FKQISREQIRQRHIIDCRGVWVR"
     gene            62466..63110
                     /gene="rffC"
                     /locus_tag="PWY43_000057"
                     /gene_synonym="wecD"
     CDS             62466..63110
                     /gene="rffC"
                     /locus_tag="PWY43_000057"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDL2134985.1"
                     /translation="MKPDQWLSDFFGREIVQVKVAADDFRQIARLQKQGFVLVEGELE
                     FELALATFSENMTACRPADMADLPDLNLLFGSAFPDSRFRSPYFSREENQRFYRTWIE
                     NAVKGKFDDIGLVQCNADGQIQGAVTVRLLAQQQAKIGLLAVKPDCRQQGIGSELLNA
                     AIAWAKQQNAKKLMITTQLSNQKAIRFYQAFGASIKAVYYWFYPALEKYDDEQS"
     gene            63097..64242
                     /gene="rffA"
                     /locus_tag="PWY43_000058"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             63097..64242
                     /gene="rffA"
                     /locus_tag="PWY43_000058"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDL2134986.1"
                     /translation="MNNPDFIPFNQPPVVGAEQAYIEQAMATGQLSGGGKFTRLCEQW
                     LENLARSKVLLTPSCTAALEMAAILLDIQPDDEVIMPSYTFVSTANAFALRGAKIVFV
                     DIRPDTMNIDERKIEQAITPKTKVIVPVHYAGIACDMDSIMATAEKYGLYVVEDAAQA
                     VNSFYKGRALGSIGHIGCYSFHETKNYTAGGEGGAIVINEANLLEKAEIIREKGTNRS
                     QFFRGEVDKYRWQDIGSSFLMSEIQAAYLYAQLIEADKINQQRLTLWHNYYQAFLPLA
                     QTAKCELPRIGEYCRHNGHLFYLKLSDLKQRTAFIDFMKAQNILTVFHYVPLHSSPAG
                     QKLGRLAGDDRFTTLESERLVRLPLFYNLSKQEQERVIQAVLAFFNQ"
     gene            complement(64287..65708)
                     /gene="glnA"
                     /locus_tag="PWY43_000059"
     CDS             complement(64287..65708)
                     /gene="glnA"
                     /locus_tag="PWY43_000059"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL2134987.1"
                     /translation="MSNAIANVSKLIQDNDIKFVLLKFTDIKGKEHGVSLPVSLVAND
                     LEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVVDPFARIPTLSIRCSIYDPNT
                     MQSYDRDPRSIATRAENYLKSTGIADSVMFGPEPEFFLFDDVRFSTEMNNVSYRIDDV
                     EAAWNTNRKFEEGNKAYRPLKKGGYCAVAPIDTAHDIRSEMCLLMEEMGLVIEAHHHE
                     VATAGQNEIATRFNSLTLKADETQIYKYIVQNVAVEHGKTACFMPKPFAGDNGSGMHC
                     NMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPTTNSYKRLVPGFEA
                     PVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPMANPYLCFAALLMAGLDGVINKI
                     HPGDAMDKNLYDLPPEELKDIPTVASSLESALDALSNDFEFLTQGGVFTKDFIDAYIG
                     IRRKEVERVNMTPHPVEFELYYS"
     gene            65895..66965
                     /locus_tag="PWY43_000060"
     CDS             65895..66965
                     /locus_tag="PWY43_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDL2134988.1"
                     /translation="MLPIYHILGSEIEHHNQQVLTFFQQELLPLLPDSEHFFYVVGKP
                     SLQTAFTHLRIKQFSSQKAIANALIAKKRQNPTACFLLHGQFNPWIWLAIALGRLPTT
                     NLIWHIWGADLYEMATSWKFKLFYPFRRVAQQKILQVWATRGDLDHLWRNMRARSEQD
                     HLIYFPTKLGDQIKISPTQNDRLTILLGNSGDPANELIQALSEIHQRLGNNVRIIIPM
                     GYPENNESYIRQVEAYSKRLFLAENLQILRKKLSFEEYVTLLSHCDLGYFNFKRQQGI
                     GTISLLISHNVPCVLHSANPFGLDLQAEKVPYLLAENLTASQIQTTKQQLKSIDKNRI
                     AFFAPGYTKLWQQRLIELMQNE"
     gene            66958..68229
                     /gene="wzyE"
                     /locus_tag="PWY43_000061"
     CDS             66958..68229
                     /gene="wzyE"
                     /locus_tag="PWY43_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDL2134989.1"
                     /translation="MNNLIPLGGFYLVSVGLFFSLLYQLYQKSGISFHLLFSLLYFVT
                     FYAGFPFSLIMAFGFDYPLPELKTQWQVFTAAGVGYLIYYVVYSRLWSAVSQPTALVA
                     NFQAKTTACILAAVAVVALLYFLYLNGFLLFKLEKYSQLFAKDRVILSALKRFFYFLL
                     PALLIFCFLYKNKTAWWALLIVGGAFGILSYVAVGGTRANLALAVGFFLLIGLQQGYL
                     SLSKVIAVSLIGVVAMFFLALARYKLTLSGSEMIYTFLYLSRDTFSPWENLARVFSNE
                     VEYQGLMPIVRDFYVYIPQSLWQERPEIVWNTANYFTKVILGNQSGLAISPTLLGSFY
                     IMGGFSVMALGMGIVAAMIKALDNLFAYAKACNSALLQAYCFGNLFNLIVLVREGIDA
                     FVSRFCFFTLIFMACYLLAKILSMGKNNNVR"
     gene            68219..68941
                     /gene="rffM"
                     /locus_tag="PWY43_000062"
     CDS             68219..68941
                     /gene="rffM"
                     /locus_tag="PWY43_000062"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDL2134990.1"
                     /translation="MFDKVIIRGIALQAVKNREIFANWLMNEKRIQFGRLMAINAEKV
                     ILSEKNPLLRRVIDEAEFNYADGISVVQSIRKKYPNYKIERIAGADLWETLMQRAGAL
                     NVPVFLVGSSADTLTKVEAKLAQWKVNIVGRQNGYFKAEQEQDVIEQIKASGAKFISV
                     AMGTPKQELFIQQLQQQYPQALYMGVGGTYDVFAGKVKRAPKIWQNLGLEWLYRLLKQ
                     PTRWQRQLNLLKYAYYYLTNQL"
     gene            69100..69176
                     /locus_tag="PWY43_000063"
     tRNA            69100..69176
                     /locus_tag="PWY43_000063"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69134..69136,aa:Arg,seq:ccg)
     gene            69206..69281
                     /locus_tag="PWY43_000064"
     tRNA            69206..69281
                     /locus_tag="PWY43_000064"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69239..69241,aa:His,seq:gtg)
BASE COUNT        20126 a        13747 c        15345 g        20159 t
ORIGIN      
        1 cggctctact ttatcactag ctgcggtaaa agagggtaaa gctcagtatg tgacccaaaa
       61 aggtttatca atgagtgcta ttacttctga ttccgcaatt aagggtaact ttgctgcaat
      121 agaaacctca gggttattaa ctgcgacggc ggaaaaaacc aatgagaatg aagtaaaagc
      181 aacagttagc cggaaaaatg tagcagacta tgtaaacgaa aacacctctg gtgatgcaat
      241 gtggaaaaat gtcgcgactg ctttagagag ctcattttcg gcattggata accaaattga
      301 aggaaataat ggttctacaa atgctacaaa tgatgaattc gcacaacaag cggctacttt
      361 acaaaacagt attgcaaaca tcaatacgca aagtgtggtt ttagatagct tatctggtca
      421 aatttatgcg tctgcgcaag cgcttacttt tgaaaactca gaaacagtga ataaagatct
      481 ctctaaccgt cttgtaatgt taggaacgct ggataatgta ggtgagactg ctggtgtgtg
      541 agtaacgggt atttatggga atggtactct aaaagaaata gggtttggtg aaggtaaaac
      601 caatacctat gctggccaaa taggttttga taagccagtt acagaccatg tgattttagg
      661 tgcggcactt aattattcta aagcaaaagt gaacttcgat cgttatggcg gtagttcaaa
      721 agcagatgga ataggtgcat cattatatgc ccgtataggc aataagcata aaacaccttg
      781 gtatttacaa ggtcgagccg gttatggcag tgttgattca gatgtagagc gtcatattat
      841 tttggcggat aataacgcaa gtacagcaaa aatcaaccac cgagacagag tgttttctgc
      901 ttatgtcgaa tcaggctatg acttcaagca aggtcgcttt gcgttaactc catttgtcgg
      961 ttttagtcat gatgttgtca gacgtggcgc attcagtgag caaaacagtc aatttggtct
     1021 caccgcggac aaagcaacct ataaacaaac aagcggattg attggtttac gaacatcttt
     1081 agaggtgaat ttagctggga tgaaaacgac tttccaaggc tatgtaaatc atcaaaaagc
     1141 gtttaatcga gaagatctta gctttaaagc aagttataca ggtttacaag atgcaaaatt
     1201 cgacgtaaaa ggtattgggc ttgcaaaaca taaaacgtgg gtagggattg gtgcattggc
     1261 ggaagtaaat aaaaatactt ccttatatgt gaattacgac cttaaactcg caaaaaatag
     1321 tggtcacaac aacgttgtga ctgctgggat tagaattaat ttctaactga atctttttaa
     1381 acaaaacggg cgtttatacg cccgtttttt tatagttata gagataataa tgttatttta
     1441 tacttcattt aattaggcaa tctttacagc ttggtaacta cactgtggat taacaatccg
     1501 atcacgagaa gcaataattt gtaactcata aagccctgat ctagcggttt gttccattac
     1561 ctcattgacc gcatttgcag tgtgttcaaa ggcttctaga tcggattttc catttaataa
     1621 gttcgccaca aatagccctg cggttaaatc gcctacaccg acaggttctt tggcaaattg
     1681 gtagagtggg cggctgatgt gccaaatgcc atcttggttg gcgaacagca tttcaaattt
     1741 gttgctctct ttgccgactc ggcttaaatg tttaaccaat acttttttcg ggcctttggt
     1801 taaaatcact ttcactgcct cgaccgcttg ctcaaaattt tccaccgtta aaccggttaa
     1861 ttctcttaat tccactaaat ttggtgtgat aatgtcggct tgtgccattg ccagattgat
     1921 caatccctct tttacgccgt ctgccacaat gcagcctttg tcggggtgtc ccattaccgg
     1981 gtcgcataca taaattgcgt ttggattttc cgctttcact ttatgaaaag cctgaataat
     2041 ttcggctact tgttctgccg agccgatata gccagagagt acggcatcgc aacgatgcag
     2101 ttcaccgata ttgtgaatgc catcaacaat gctaccgatt tgttcttttg gcatcaccat
     2161 accgccccat tttccatatt gggtatggtt ggaaaactgt acggtattta acgcccatac
     2221 atcaacacct aacagctgca taggaaaagt tgaagcctta ttgccggcat agccgtagac
     2281 aacgtgcgat tgtatggata gtatgttttt cattcattca ctcctttaaa atttcaatat
     2341 gtgttgtttt ttagtcattt gaagttgcaa ggacttgaag ctcatcaaga aatctctata
     2401 atcccgcctc gttctcttat ccattcaata tgccgactta gctcagtagg tagagcaact
     2461 gacttgtaat cagtaggtca ccagttcgat tccggtagtc ggcaccatca cctttatttt
     2521 ctgatgagtc ccctttaatg taaaatggca agattttgca aattttcaca aaaatcttac
     2581 cgcttgtagg gtgaaattga attagacgta accgtaattc attaagcgtt gataacggcg
     2641 ttccagtaaa tcctctttat taagttgatc taaccctgcc aaatcttcaa caatacgctg
     2701 tttaaggttt tgggcgattt gttcaacatt gcgatgagca ccgcctaagg gttccggcac
     2761 gatgttatca atcagctcaa gctcttttaa acgaggggca gttaaaccca ttacttctgc
     2821 tgccgttgag gctttatcgg cacttttcca cagaatcgag gcacagcctt caggtgaaat
     2881 taccgagtaa gttgaatatt gcaacatatt gaccttatcg cccacaccga tcgctaatgc
     2941 accgcccgag ccaccttcgc caattaccgt acagattact ggcacggaaa gttgagacat
     3001 ttcacgcagg ttacgggcaa tcgcttctga ctgaccacgc tcttccgcac caatgcccgg
     3061 ataagctccc ggtgtgtcga taaaggtgat gattggcata ttaaagcgtt ctgccatttc
     3121 cattaaacgt aacgctttgc gatagccttc aggagcaggc ataccaaagt tacgtttcac
     3181 tttctctttt acggtacgac ctttctgatg accgataacc atcaccggac gtccgtccag
     3241 acgggcaata ccaccgacaa tcgctttatc atcggcaaag gcacggtcgc ctgccagttc
     3301 gtcaaattca gtgaaaatat gctcaatata atctaaagtg tacgggcgat tggggtggcg
     3361 tgccatacgg gaaatttgcc acgcatctaa attagcaaag gttttcttgg ttaattcctc
     3421 gcttttcttt tgtaaacgct tgatttcatc atcaagatta attttgtcat cttgctcgct
     3481 gactgctctt aaagactcga tcttggcttc caattcagca atcggcaatt caaaatctaa
     3541 aaattcttgg ctcattctat tttccttttt atgggcaaaa ttatggggca ttattgctta
     3601 tcagtcggtt attgtccatt ttcaattacg ctaaattgac ccatcgtacc tctatcacgc
     3661 agcataaaat cgctcacgcc aaaggtaaat ggcagttgct caggggcagt attatcaaag
     3721 cgaaccaaca gcgtgatttc ttgctccgcc tcgagccaaa cggtgtcccg ccacgcaagt
     3781 tgtttgatag ggtattgctt gcggtttagg gtttcaataa tgaattttgc accttgtagc
     3841 gtaaatccga ccgcttgtgt gctatgtaaa taccaacgct caatgctgcc tttttgcgcg
     3901 gtaaaatcaa tgcgttttgg gtcaaaccgc ttttggttga ttaaataatc agccggacga
     3961 atggcaaaac gtctctcttg gctgattttc aggttaaatt cctctaagtt gaaagccggt
     4021 aaactcggtt tattattcaa taccgctgcc attccttcag ggcgaagctc taaaatcacg
     4081 ttatcattca attcgtcgtt gtcagcaaag acctgcccga ttttgtaaaa aatatctctt
     4141 ttttgaccgc ttatcagcga taccgctttc ccttcgctta aatcgaccaa cacttccacc
     4201 ctttcactcg gtgcaagttg aaccgattcg gtttcaagtg gctctgccag cattcctaag
     4261 ccggtggcga ttaggtacaa cggctgctcg ttatctaagc gtaattcata agcacgggaa
     4321 agtgaggcat tgacgatccg caatctgacc cagccacgag gcacgttaaa ataaggcgat
     4381 tcctgcccgt tgacaaataa tcgcttaccg aaaaattgtg aggtgttttt atctaacacc
     4441 tgtacgccgt ctttgttgag aagctggtct tgcagaatta acggaatgtc gttcacgcca
     4501 tatttattcg gtaaagtggc ttttttgctc tgttcatctt caatcatcca caagccggct
     4561 aagccacgat acacttggaa tgccgagttg agcattgtat cggcgtgata ccaacaagta
     4621 caagccggtt gattgataga aacaatcggc gaccagctac ttttggggtc aagggaacga
     4681 tgcaccgtgc caatcatctc acttgaggct tgcaatcctt gaatattcat tgagattttt
     4741 tgcggtaaat tattgagata agttaatttg acaaaatccc ccgattttac ccgtacggtt
     4801 ggggcaagat attgcccgtt cacaccccat acatcaacca atttgccttt atcaaattgg
     4861 gtttgtgcgg ggcgtaaatc caaccgaacc ggctttcctc gcccaatatc cattaaaggc
     4921 ggaatgttca gttgcggtcg ctctgctgct aatagcggtg ttggcactgt tgcaaatcca
     4981 gccgcaaggg cagagccttg taaaaattgg cgtcgggtga tctgtttttt catattaggc
     5041 gttttttgca gtgttttcta actgttcgac ttcttgatta agttgttcaa attttgccaa
     5101 cataatttga tggcactttt ccatcagttc tttgacgttt tcacgggaat agcctgaagt
     5161 atcaatcgga tcaagcattt cacagattac tgtgccgtta tttgaacggt tcaaatccac
     5221 tttattgtgt aaattagagc aaactaccgg cacaatcggc acgccggcag aaatggctaa
     5281 atggaatgca ccggttttaa atggcagtaa accacgccca cggctacgag taccttccgg
     5341 gaacatccac accgacacat tgcgttcttt cacgatttgc cctgctttat tcattgtttc
     5401 aatcgcactg gaacgctttt cacggtgaat aaaaatattg ccggttgccc agtaaaccaa
     5461 accgaaaaac ggaatccaaa tcaggctttt tttgccgata ctcaccgtct tttctgccac
     5521 cattgaggaa atcgttacca tatcgtaatt gttttgatga ttgccgatat agacaaccgg
     5581 atagttaatt tccggctttt tgcgggtaat taattccaac ccgactaatt tatgcaaagc
     5641 accaaaccaa cgagctacca cacccacgct acttgggtga cgtaaacgca gtaacgcata
     5701 aatagtgcca aaaatagaaa tgaaaatagc ggcaagggca acaattaaaa tacgaaaaat
     5761 ttttaacata atggaataac cttaaaaata aattgtctag gagtatagca aaataaggtt
     5821 attttctaaa ggctatccac attaaattga tagtttccta ttttgcaaaa aatcggtata
     5881 tttaaaccgc ttgtttattt gataaggaga aaaaaatgag taaagtgact ttagcaggaa
     5941 gcccgattga tgttgcaggc acatttccac aaaaaggcga tgtggttgcc gattttacct
     6001 tagtcaacag tgatttaagt gatgtttcgc tttccgattt tgatggcaaa cgcaaagtgt
     6061 taaatatttt cccaagtatt gacaccggca tttgtgccac ttctgtgcgt gtgtttaatc
     6121 aaaaagcggc tgatgtagcg aatacggttg tcctttgcat ttcagcggat ttaccttttg
     6181 cccaagcacg tttctgtgga gcagaaggaa ttgaaaatgc caaagtatta tccagtttcc
     6241 gtcatcgtga tttacacaac aaactggggg tagatattat ttcaggtccg ttggcaggct
     6301 taacttcacg ttcagtaatt gtgttagatg aaaacaacaa ggtattacac agcgaattag
     6361 tgccggaaat taaacaagag ccggattatg aggcggcgtt agcggtatta taatttgcag
     6421 aattatgctg gaaaataacc gcttgtaaag caacaaaaag cccgttgatt caattcaacg
     6481 ggctttgttt ttagtgatta tttcttacga gagtgtaagc cttttttctc aaggttacgg
     6541 taaatcaacc attgctgtgc aatcgtgatt aagtttgagg ttaaccagta aagcactaag
     6601 cctgatggga accataagaa gaacacggta aagataaccg gcataaaggt cattactttt
     6661 tgttgtaccg ggtcagctac cggtgttggc gacatttttt gtaacaagaa catcgaacca
     6721 cccattaata aaggcaaaat gtagtatggg tcttgtgccg ataagtcttg aatccagccg
     6781 aagaaaggtg cgtggcgtaa ttctaccgct tccataaatg tccaatataa agcaatgaaa
     6841 atcggcattt ggattaaaat tggtaagcag ccacccattg ggttcacttt ttcttctctg
     6901 tagagtttca tcatctcttg gctcatacgt tggcggtcgt cgccgaaacg ttcacgcatt
     6961 tcagtaatgc gaggttgtaa catacgcatt tttgccatcg aggtgtattg tgctttggtt
     7021 aatgggtaaa gaatggtttt cactacaata gtgacaccga taattgccaa gccccagttg
     7081 cttacgattt tttgaattgc ggttagtaac cagaaaagcg gtttagcgat aaaccacgcc
     7141 cagccgtaat ctacggttaa gtctaggtga tttgctgatt cgcccatttc tttctgatct
     7201 ttagaacctg tccaaagttt gctatttaaa ttagcctctg aatttggcgc aatcgtagtt
     7261 aaagggccac ggtaaccgat agttgctacc ccattattgg tgcgtgaata aaggctgtta
     7321 tccgcatctt ggtttggcac ccaagcagac acgaaatagt gctgtaatac cgcaatccaa
     7381 cccgctttgg tgtccacgct taagttggct ttttccatat cttggaaact gtattttttg
     7441 tagttggttt ctgaagaaga ataagccccg ccggtgtaag tcggcatggt taaattgccg
     7501 gaactttcaa ctatagagtg ttttaattgc ccgtaaggct gaacttcaat cgcttgtgca
     7561 gtattatttt tgatattgaa gtttacccca acatcgtagc taccacgttt tagcgtgaaa
     7621 gttttggtgt aaaccacacc ctctttttca aaggttaatg gtacagaaat ttcattttgc
     7681 ccttcggcta atttgaagct atctgccgtt accgcatatt gtgggcgacc ggcattggta
     7741 tcaatcccgt ttttacccac taaaccgctt tgagcaacgt agattttgtc gccgtcttgc
     7801 tgtaatagtt taaatggcgt gttagagtgt aattcagcat tgtgttttaa caaatctgag
     7861 ccgatgacat caccgcctaa ggtatcaata gtcagacgta atacatcgct ttccacggta
     7921 atggttttac cttgcgtgat gctatctgcg attggattat tggcattgga agcagacgga
     7981 agatcacccg cttgttgggt ttgtgctgcc acttgagctt gtttttgtgc ttgaatttca
     8041 ggatcaaaat cttgttgcca ttgtgtaaaa atcaaaatag acacaagaaa taaacccatt
     8101 agcagtaagc cacgattatt catgttggaa ttctcttgtt agtaatggat aaaaagaaag
     8161 gtattttata ataggttagc ttgttttcca aacgcctttt atgaacaagc ggtcggattt
     8221 tccaagtttt ttgcaaaatt ttcggagaat ttgaccgctt gtggagaata agaaactaac
     8281 gtttatctaa ggttttggtg gaaaggcgag caactgcaca caggttacct tgttcgtctt
     8341 taatgtcgat attccaaacc tgtacttcac gccctaattt cagcggtgta gctttggcaa
     8401 tcgcttttgt gcctgccgga atcgatttta aatggctgat gtttagctcc attcccaccg
     8461 caatttgatg gggttcacac accattaacg cccctgcatt ggcagtagtt tccgcgagtg
     8521 ctgccgatac accgccgtgc aatacgccaa acggctgttg agtgcgttcg tccacctgaa
     8581 gctgtgcttc aatccaatct tcaccgatag cggtaaattc aatccctaag tgtgacaccg
     8641 ccgatttttg gctgagtgtg tttaattgtt cacaagttgc tgtttgcttc caaatggtca
     8701 ttattatttc ctgtctaata aaaaagctgt ctatttagac agctttcatt atctctttga
     8761 gcgaaacata ctacgccaat agtacattgt ttggtcgccc atattacctt aagccatata
     8821 gcactaagga ggtgatttat gtgagaaaat cagaagtttt ctttctggtt gcgttgctaa
     8881 tattattagc aacttccgct agcattaaag tactcaccat acttgatgtg ctagtttggt
     8941 tattcgctag ataacctaaa atcagacaga ggcaggctgg ttacctgcct cgctttttat
     9001 tctaagtcct tttaggttac atttcaacct taaatcacgt ccagcaacgc ttgaattggg
     9061 tgtttaatgc cttgttgttc aaaacgtttg acttgcgagc ggcaggagta gccggttgct
     9121 aggcaacgct caagcggttt attttttaat ttaatttgcc atgattgctg ataaatcgct
     9181 ttcgacattt caacgtgttt ggtttcgtgt ccgaacgtgc ctgccattcc acagcaaccg
     9241 acattttcat tgagtaaggt ttcaccaaaa tgggcgaaaa tagcttgcca ctctttcatt
     9301 gagcttggca aggcggtgga ttcggtgcag tgagcaaata aataccacgg caagcggtcg
     9361 gaatttgccg attttttgca attttccagc tcgccgtttt taatcacttc ccttaaccat
     9421 tcgtgtgcca ataagacttt gaaatccccc cgagcttctt tgaggatctc tttgtattct
     9481 tcacgataaa gcaaagttaa ggcaggatcg acagccacca tcggtagccc caatttcgcc
     9541 acacggttta aaaaatcggc ttggtttttc gctgttttcg caaatttatg caaaaagcct
     9601 ttaacgtgtt gtgctttacc acttggcttg aatggcaaca caatcggctt aaagcctaat
     9661 tttttaatga gagcgataaa gtctgccacc actttggcat cgtaataaga ggtaaacgga
     9721 tcttgcacaa taaacaaggt tttgcaattt tttgcagaaa aatcaccgct tgtagcttgt
     9781 tgttcaaggc tttctaagct ctcgccttgg tagccgactt ccaccaactg atgttgcaga
     9841 tttggcactg acaatgccgg caaataggtc attccaatgg ttttgtttgc agctttttcg
     9901 gcaaaagttg aggtgctgaa aaagttaaaa aaacgtggtt gtttcgccat taaaggggcg
     9961 ataaattcta agtttgagac cacataatct ttcagtggtc gcaaatagcg gctgtggtaa
    10021 agctcgttaa attgcgatct gaacgtcggt acgtcaattt tgattgggca ttggctggca
    10081 caggctttgc aggcaaggca ggtgtccatc gccgctttga cttcgtgtga aaaatcatac
    10141 tctttgtttt tattgagcgt gttgcgaatt tttgcaacaa aatcagctaa tttgaccgct
    10201 tgtttgcgag gtttgaagat caaatcgtcc ggcgaaacgt tttgttcggt aagtaatctg
    10261 agccattcac gcacaagagt agcccgccct ttcggtgagt aaagacggtt gcccgagact
    10321 ttcattgaag ggcacatcgt gctgtgtaca tcaaagttaa aacatagccc gttgccgtta
    10381 caattcattg ctcctttaaa ttcttctcgc acttggattg gaatttggcg gtcaaaatcg
    10441 gctcgcattt gtgagtcaat cgggtagagc gaggtttcgc tttctaatgg tgtacaaatt
    10501 ttacctgggt tcaagcgatt gtacgggtca aataaggttt tgatgtaacg gagttcttgc
    10561 cacaaagttt cgccaaagaa acgttcgccg tagtaagaac gcataccttt gccgtgttcg
    10621 ccccaaatca agccaccgta tttggcggtg agttcgacta cttggtcgga aatttgctta
    10681 aaggtttcca cttgggcttt gtcgcataag tcaagagcag ggcgaacgtg tagcacgccg
    10741 gcatcaacgt ggccgaacat tccgtaaggc agattatagc gatctaataa agctcgaaat
    10801 tccacgatgt aatcggcaag gttttccggt ggcacagcgg tgtcttctac aaaggcgatc
    10861 ggttttgccc agcctttggc attgcctagc aggcctacgg cttttttccg cattgcgtag
    10921 attttctcga tagaatttac atcactcgtg agttggtagc cgataatccc ggcttcatta
    10981 tttgccattt tttgatcaag gctttggcag agtgctttca cttgggtttc aatttgagtc
    11041 tgatcgcttg aggcatattc aacaatgtta atccctaaaa tcgggttggc tgggtcttcg
    11101 gttaataaat ctgacaccga atgccagacg atatcttgct tggcaagatt gagcactttg
    11161 gaatcaaccg tttccaccga taaggctttt gcctctaaca taaaaggagc ggagcgtaag
    11221 gcggcatcaa aggaattata tttaatatta attaaggtgc gatatttggg aatcggcagt
    11281 aaattcagtt tggcttcgca aataaaggca agcgaacctt cagaaccggt taaaatacgg
    11341 gtaaggttaa attcgctttc accatggtta aacacatttt ttaaatcata gccggtaata
    11401 aaacggttaa gctgaggcag ttcgttcagc actttggggc gtaatgtttt gcaacggttg
    11461 aaaatttcac ggtgcaaggt tttgccgaga gcggagagat ttaacccttc aatatgtgca
    11521 aaaaaatcgg cagatttgac cgcttgtgtc tctaaaatat cgccgttaat aagcacagat
    11581 ttgatggcaa gaatatgatc agatgttttg ccatattgca aagaaccttg cccagaggca
    11641 tcggtgttga tcattccgcc taaagtcgct cggttactgg tagaaagttc gggggaaaag
    11701 aaaagtccat gtggttttaa aaattgattg agctggtctt tcaccacgcc agcctgcact
    11761 cgaacccaac gctcttccac attcagttcc aaaatctgat tcatatggcg ggagagatca
    11821 acaataatat tattgttaag agattgcccg ttagtgcctg tgccaccgcc tcgtggagta
    11881 aaagttaaat gttgatattc tggcttttgg gcaagtttgg ttagaattac gacatcagaa
    11941 acggatttcg gaaacagaat agcttgcgga atttgctgat acacgctgtt gtctgttgcc
    12001 agtgaaagcc tatctgcata atttgatgca atttccccac taaaatgctg attgacgagt
    12061 tcagttaaat aggattgggt ggctttatcc agttgcggta cttcagagag tctaggtatc
    12121 ataagctgtg tttggttttt tataataaaa gcactaaatt ttgcttatct tagcaatttt
    12181 tctaaaaatt gggaatggaa tatcaatttg attaaatagt gttcgaaaaa tggtttttgg
    12241 ttaaaatata gacaaattat tacgtttcta tacatttcat caggaaaaaa cgattgattt
    12301 ttcgaaaatg aaaccacata atgacaatat tcttgctcaa tctaaattag aacaagaatg
    12361 ttactcagaa gataaatttt atacaaaaaa tctgttttta gataaatctt tgtataatag
    12421 cttctgacat tagctatctt gcttcttatt ataatgttga agcataaagt taaagtttta
    12481 atactttttg tgggtattaa gaatttattt taatcgatga caatatttaa gaggatcatc
    12541 aaaatgaaaa aaacattagt tgcattagca gtattatcag cagctgcagt agctcaagca
    12601 gctccacaag ctaacacttt ctacgcaggt gctaaagctg gttgggcatc attccacgac
    12661 ggtttaactc aatttgacca cgaaggagat ggtggtggat tcgatcttaa atacggtatt
    12721 aaccgtaact ctgtaactta cggtgtattc ggtggttacc aaatcttaaa ccaaaacaac
    12781 tttggtttag cgacagaatt aggttatgac tactttggtc gtgttcgtgg taacactaca
    12841 tttgctgatg ataaagaaag aactgcggct aaacattcag ctcacggtgc gcacttaagc
    12901 ttaaaaccaa gctacgaagt tgttcctaac ttagatgttt acggtaaagt aggtgttgca
    12961 ttagttcgca acgattacta cgtacaacaa cgtgtagata aagattcacg cattaaagct
    13021 cacaacttaa aaccatctct attattaggt gctggtcttg agtatgcaat tactcctgaa
    13081 ttagctgctc gtgttgaata tcaatactta aatcgtgttg gtaacgtaga taaggcagtt
    13141 cgtaaaacag ttagtattcc aacaggtaca aatttccaat acagcccaga tatccactca
    13201 gtatctgcag gtttatcata ccgtttcggt caaggtgcag caccagttga agcaccagaa
    13261 gttgtaacta aaaacttcgc attcagctct gatgttttat ttgatttcgg taaatcaagc
    13321 ttaaaaccag cagcagcaac ttctttagat gcagcacacg ctgaaatcag caacttaggc
    13381 ttagcaaacc tagctatcca agttaatggt tacacagacc gtatcggtaa agaagcatct
    13441 aacttaaaac tttcacaacg tcgtgctgaa acagtagcta attacatcgt ttctaaaggt
    13501 actaacccag ctaatgtaac agctgtaggt tacggtgaag caaacccagt aactggtcac
    13561 acatgtgatg cagttaaagg tcgtaaagca ttaatcgctt gcttagcacc agatcgtcgt
    13621 gttgaaatcc aagttcaagg ttcaaaagaa gtaactatgt aatatagtta ttcagttgaa
    13681 aaattgaatc aaaaaaagcc cgcttgttag tgggcttttt tcttttttta tttcagtaaa
    13741 tcttttaaac ttgatttcat tttagcaact tgctctaagt aaagttgttt attttcgagt
    13801 tcatcggtta atttgacaat agcttcagaa agcgttagtt tttggctttt ggcatattta
    13861 gagagcctta accagctcga atactctaaa tcaatggatt ttttcttggt tgtttgcttt
    13921 tcagcattaa agaagcgttt gcgtttggcg cgtactgatt gacgcatttt cttacgttgc
    13981 tcaggcgtca tttccgactt aatccatttt tccacttcgt ccggagagtt ttgcaaagta
    14041 gagaggaact gaacttttaa atcgatcaag ctttgttctt cgtacttagt aatattttct
    14101 ccttcacgat gtttttttag gagatattgc catttccaag ttgtttcttg ggtgatgagt
    14161 ttctgatatc gcataatctg aatatgatag gaaaaagtaa gctatattta gcaagtatag
    14221 cggaattttg tataataaac caaattttca ataatgaggc agatttgtga ccctttcttt
    14281 acttagtagc caaaaattag taatgcaaaa tgatttctct cgtttagatg agaaattgaa
    14341 ttttttagat tttcaacctc aagcaaaaca agcgttaaat caatttttac aatctcagtt
    14401 tggtgcttta ttagtcttaa aatctgagat ttttccggaa tttatcaatg aaatcacgga
    14461 atatctgaaa aaatatagtg tagatctgcc gattattagc aaaatggatt tcaataaaaa
    14521 tagtctattt ggttattctg tgtttttgga aagagagctg aaaacagaat atgttaaagg
    14581 tgcaattcac gaggcagata agggaatttt aattgttaat atccgttcgc tcttattgga
    14641 tattacccaa tgggataagc taaaacaggc attattattt ggtgaatatg agccaaattc
    14701 tgttaatcac tcaccctatt tatcggctaa agagaaatca acatttaaat tgattttagt
    14761 aggggaaaga gaagatattt ctactttatc tgcttatgat gatgggttgt accaatttgg
    14821 gcaatatacc gaaataaaat cctacttatc attatcggat aatgtggagc agtggggagg
    14881 ttatgttcag aattatgctg agaatctcat caataagcga ttaacttcca cagcgcttaa
    14941 tcaattttta caggcctata tacgtgaaag tgaaagccga gagttggtgt ctatttcccc
    15001 tacattattg agaaaacatt tattaggaat tcaaaatttt tacccaaatg tgaccgaaat
    15061 tgacgaggta aaaggttatt ttgattattt agaacagcaa tcatcgatat taaatcaata
    15121 tacggtgggt gatattctga ctaatcaact ttatattgaa accgaaggag aggaaattgg
    15181 gcagatcaat ggcttatcgg tcattgagtt tgaaggcatt ccgcatagtt ttggcgagcc
    15241 cttacgcatg agttgtaatg tacaatatgg tgatggcgaa attacagata ttgagcgtaa
    15301 agtggagcta gggggaaata tccactccaa aggtattatt atcgctcaat cttgtttagc
    15361 gaatctgtta gaactaccaa cccagttacc gttttcagct tcgattgctt ttgaacaatc
    15421 ctatagtgaa attgatgggg atagttcttc tcttgcgatt ttcaatgtac tagtgagtgc
    15481 cttagctaaa gttggcttgc cacaatctat tgcggtgaca ggtgctatcg atcaatttgg
    15541 gaatgtgcta agcgtgggcg gggtaaatca gaaaattgaa ggcttcttta atatttgtca
    15601 cgctcgccaa ttaacgggga aacaaggtgt gattattccg gcggcctgtg ttagccattt
    15661 gagtttaagt tcagaagttg ttgaggcagt ggagcaaggt ctatttaaaa tttggacggt
    15721 ggaaaatgtg tttgatgcca tagcgattct gtttgagcgg gatttttatg atgatgagaa
    15781 agcccctaac tcagcccaaa cagcattatt taccttgatt caccagaatc tagatgagcg
    15841 ggaaagtgaa gaaacaagcg gttcattttt aagcaaaatt tgcaaaaaga taaagctaga
    15901 ctgatccgac aagtcagctt acaactgttc actgaattaa aagtctgtta ctatcagcct
    15961 gatttatcag ttattgatat aacaggcttt tttcacttta caggatttta aaaataatga
    16021 acagttgtac accaattatc aaaccaagct atacttacga agagcttttg ctatcaggaa
    16081 aaggagaatt attcggtgaa aaagggcctc aattgccggc tccaccgatg ttaatgatgg
    16141 atcgcatcat tgaaatgaac caagaaaaag gcaattttga aaaagggtat attgaagctg
    16201 agtttgatat taaaccggat ttatggtttt ttaactgcca ttttattaac gatcctgtaa
    16261 tgccgggttg cttaggctta gatgcgatgt ggcaactggt tggtttttat ctcggctgga
    16321 ttggtggcga aggcaaaggg cgtgcattgg gcgtaggtga agtaaaattc accggacaaa
    16381 ttttaccgac agcgaaaaaa gttacttacc gtatccatat gaaacgtgtg gtcaatcgta
    16441 agttagtgat gggggttgct gatggtgaag tcgaagtgga cgggcgagtg atctatactg
    16501 ctactgattt aaaagtaggc ttattccaag atacgacagc attctaatcc ttataacctt
    16561 ataaccttat aacaaaaaga gctacatttc gtagctcttt ctgctttttt aacaacgttt
    16621 gattagcgat tcaaccacgc catatattcc gctgttcctt cagcaacggt tttaaatgtg
    16681 cctgtgtagc cagcggctct taggttggtt aagtccgctt gagtgaacgt ttggtagcgg
    16741 gattttaaat gatccgggaa tgggatggtt tcaatttcgc ctttgccgtg gtatttgata
    16801 accgcattcg caacttcttg gaaagattcc gcttttccgg tgccgaggtt gaaaatgcca
    16861 gaaatgctgt tttgccacgc ccaaagattc acttttgcca catcttccac ataaacaaaa
    16921 tcacgcaaga actgctctga gccggcaaat agttttggat tttccccttt taacatctgg
    16981 ttgtttaagt ggaatgccac gcttgccatt gaacctttgt gttgctcacg aggcccgtat
    17041 acgttgaaat atttgaaacc acagaccggt gattctgcct caggcaaaat acggcggaca
    17101 tattcatcaa acaggaattt ggagtagcca taagcattta atggaccttc aaactcccgt
    17161 ttctcgataa aattatccga acgtccgcca taggttgccg cacttgaggc atagaagaaa
    17221 ggaatttggc gatctaagca aaaatgcaat aactcttttg aatattcgta gttattttgc
    17281 attaagtatt tgccgtccca ttcggtggtt gccgagcaag ccccttgatg gaaaaccgca
    17341 tcaatctcac caaaatcatc gccggcgata atagaggcga tgaaatcctc tttatcgcaa
    17401 taatccgcaa tatctaagtc cactaagttg ataaattttt ccccgttttt gaggttatct
    17461 actactaaaa tatctttgcg accgatttca tttaaggctt taacaatgtt actgccaatc
    17521 atacctgcgc cgccggtgac gataatcata tttttatcct ttctaaagtt gaactgtggt
    17581 tattatcgtg tcatcacact taatttgcaa atttttaagc cattttaacc gcttgtgcct
    17641 ggttatttcg ctcgtgcaat ttgctttttc aatttttgca ttttttcttt gatgcgttgg
    17701 cgttttaaat tggatatgtg gtcaacaaaa agcacgccgt ttaaatggtc gatttcgtgt
    17761 tgaatacaaa tcgcaaataa atcatcggca tcgatgataa tttcttcgcc ttggcggtta
    17821 agggctttga ctttcacttt ctctttgcgt ggcactaagg cacgatgccc gggaatggag
    17881 agacagccct cttcaatgcc ggtttcaccg ctgctttcta aaatttcagg gttaattaac
    17941 accacttgat tggttttgtc gccctcaata tcaatagtaa taacacgctt aagtacgccc
    18001 acttgcggtg cagctaagcc aataccttcg tgttcgtaca ttgtctcaaa catatcatcg
    18061 ataaaacgat tcagtgcttc gtccacctgt tccaccggtt cacaaacggt tgccagtttc
    18121 tcatcggggt aaagcacaac atctaatatt gccatttttg tgatctctct cgaaaagttt
    18181 aaaaaattcg tttgtctatt ctaaacgttt tcttattatt agggaaattt tgagggcgaa
    18241 aaaatggcat atcagtatga tttattgcgt ttgttgcagg tgcaaattgg tccgcagcga
    18301 atcggacggt tattggcaga gttggatttt gccgaatttt gtgcctacga taaaacacag
    18361 ttaaggcaga tcggctggaa tgaaaagcag attcagcgtt ggtttaatcc ggataaaaat
    18421 tggattgagc aggcactaac ttgggcggag cagccgaatc agcaaatcct gagtttattt
    18481 gataaagctt atcctttctt gctgcggcaa atttccaccg ctccgccgat tttatttgta
    18541 aagggtaatg tcgaaagcct ttccttaccg caaattgcga ttgttgggag tcgggattat
    18601 tcgccctacg gtgaatattg ggcggggcaa tttgccgctg acttggtgaa gcacaatatg
    18661 gcggtgacta gcggtttagc gattggcatt gacggttttg cacataaaaa agtagtggaa
    18721 tatcaaggta ttacgattgc tgtgttgggc agtggcttaa accggattta ccccgcacgc
    18781 catcaaaaac tggctgaaca aattgtcgag tcaggtggtg cgttggtgtc tgaattttca
    18841 cctaatcagc ctccgattgc tgaaaatttc ccacgccgaa accgcataat cagcggttta
    18901 tcgctcggca ctttggtgat tgaagcaacc attaatagtg gctctttaat taccgctcgc
    18961 tatgcccttg aacaaggtcg ggaggtgttt gcgttaccga atgcggtgca aaacccttac
    19021 gcacaaggtt gccacaagct aattaaagaa ggggcgttgc ttgtggaggg ggtggaagat
    19081 attttagagg cggtcgcctc tcaatatcaa cctcaacaaa tccctttgtt tgatgagcct
    19141 aagccaatag taagcacatc tttacaagcg gtcaaaagta gcgaaaaatt tgcaaaaaat
    19201 ccgccaaatc tgaccgcttg ccagcagcaa atttatcagc atatcagctt agagcctata
    19261 gcgattgacg atttagccag agctacagag ttaacgattg agacattgct agttgaatta
    19321 ctggggctgg aattagccgg cgtaattaaa caagtgagtg gggggtatgt gatggataat
    19381 gggtattgaa caagagcgtt gtcgtttaac taaacaaaat tccctaccat caataaatgg
    19441 tagggaagat tcctcgcagg tagcaaataa catctgattt taattaggct ttcggctctg
    19501 ccggtttttt cgccaaataa ttataggcta ccaacagagt gaataagccg ataataatga
    19561 gcgtaatgat attcttatcg aagtattgag ccatatagcc taacacaatc ccaataccac
    19621 cgaagtctga gtcagagaaa gtggtgttag caaagcctaa tgaacctagt acaggtaata
    19681 agaataccgg taagaaagta attaatatac cgtgaataaa agccccgata attgcaccac
    19741 gacgcccgcc ggtggcgttc ccaaatacgc cggcagttgc cccacagaag aagtgcggca
    19801 ctacgccagg taaaattaac acccaattaa gttgtcctaa aatcgctaag cttaaaatcc
    19861 cacctaagaa actggcaaga aatccaacta ataccgcatt gggagcataa gggaacacaa
    19921 tcgggcagtc gagagccggt ttggcatcag gtactaattt ttcggagaag ccggtaaatg
    19981 ccgggacgat ctcagctaaa attaaacgaa ccccttgcaa aatgacataa acaccggctg
    20041 caaacgtgat ggcttgaatg gtggcataca ctaaataatg ttgtccaccg cttaatgtgg
    20101 aaacgtattc actgcctgaa gcaatggcga gaatatagta aatcgccatc atcgtgagtg
    20161 aaatagaaat ggagctatca cgtaagaagc ctaggttttt aggtaaatcc atttcttcgg
    20221 tagatttaga gccttttcct actaattgtc cgattgcacc agcgagcacg tagcctaatg
    20281 tgccgaaatg acctaaggca acatcattgc taccggttac ttttttcata tagaagtgag
    20341 cgatagtcgg gaaaaatgcc ataattaacc ctaaggttaa tgccccgaca aagacgagct
    20401 gaataccttc aaagccggca acggttagga ttacgccgat catacacgcc atatagaagg
    20461 tatggtgtcc ggttaagaaa atgaatttta ggcgggtaaa acgtgcaatt aaaatattgg
    20521 ctaccatacc gaatgccata attaacgcag tcgccgtacc atatttttcg attgccatag
    20581 acacaatcgc ttcgttatta gggataatgc cttgcacgtt gaaggcgtgt tcaaacatac
    20641 cgcctaaagg agtcagggag cttaaaagta ctgttgcacc accgcttaac actaagaagc
    20701 ctaaaatagt tttgattgtt cctttaacaa tatcggatac cgattttttc tgtgcaacta
    20761 aaccgaccag agcaattaaa cccactagaa cggaaggcac tttgagaata tctaatataa
    20821 aaaagagtag cgattccata taacatctcc gttattggtt aaagtaagcg gtcaattttt
    20881 cttcgaattc tgctaatcca acaatgtttt ttaccacgat gagtttttct ttcggcacat
    20941 tgctgcttgc ggcaatatct tttgccatca caaataaatc agcatcatca gcaacaacag
    21001 aggaaaggtc ggtatgactc acttccgctt cgacaccgag ttttgccaat acttttttaa
    21061 tgttcatttc catcataaag ctgctaccta agccggaacc acatactgcc ataattttca
    21121 tagtaaatct ccattaaagg tgaatgaata gttgtctgaa ttttgaacta tagaaatact
    21181 atagaataga aatgttaacg ctatcaaaca gaaaatatta aaaatgtgat agcgttagca
    21241 tttttagtat ttaattagat aaaaaataat cagttggcat tttgctctgt tctttctaaa
    21301 taggaaaatt aaggatttag ccctataata tggaacaatt aggaaaatag tatggcaaat
    21361 ggaacaagta gaaatcgaag atcttcgggc agagtcactt tagctgatgt agccaaagtt
    21421 gttggtgttg ggcagatgac ggtatctcgt gctttgcgta caccagaaat ggtgtcggag
    21481 ccactacgta aacggattga agaggcaatc attgaactag gatatattcc gcatttttct
    21541 gctcggcaat tagcttctgc agaatcacat aatttggtca ttgtgacgac gtcgattagc
    21601 tcaacggaaa ataccttaat tttatcggca ttacgcaaaa gcgtggcgga ttccgcattg
    21661 caaatggtga ttttgatcgc tgatcaagaa tcttggtttc aagaactgat taatcatgct
    21721 cctcaagcga tagtgttttt gaatttagat tgccctccta atgtccgtga gtggattaaa
    21781 aacagcaata tccctaccgt agaaataggt gcgataaaaa aagaaccgat tcatctcagt
    21841 gttggaattg atagccacga agcaatggag aaaatgatcc attttttggt gaaaaaaggc
    21901 tatcgagaga ttggattgtt atcggcgaag caagatttat ccatcttcaa acaatattta
    21961 gaaagttggc atacgacttt atttgcacat catatttctc ctttaaaaat ccaccacgta
    22021 gtagaggata tttcgttttc ggcaggagca actttgttga ctgaagccct tctaaattgg
    22081 gaagcaatag atgcattggt ttttttatcc gatgaattgg cttgcggagc attatatgaa
    22141 gcattacgcc gacatatgag cattccaaaa gatcttgcga ttgccggttt aggtggttta
    22201 gaagtgggga gtatttccta ccctaaattg acaaccattg cgatccctta cgagcaaatg
    22261 ggaaaaatag caggtaaaca actacttact cttttgagtg gtaaaacctt atcagcagaa
    22321 gagatgattg ttaaattacc gactaagcta gtgattcgtg atagcaccgc ataatattga
    22381 ttttgcataa aaatctataa aagatcgtgg caagtcttgt agaaaatgat agaatttaac
    22441 gctttaaaat aatttctgat ttttttattc attttttagg ttatatatgg ctactgagat
    22501 taaaacaaaa acccccgagc aagtcgaaca aaaagggggg aaaagcaaag gtgcaaatac
    22561 agcattatgg ctggttgcga ttgtattttt agcagttgct gcggtaggta atgcctattt
    22621 ttcatctcat ttttcattaa ttgttcgagt gcttttattg gtggtgttgc tagttggtgc
    22681 tgttgcacta gcggcaatta caaatcaggg gcaaaaggca attcatttta ttaaagagtc
    22741 tcgaaccgaa ctacgcaaaa ttatttggcc gacacgccct gaagcaacac aaaccacctt
    22801 gattgtattg gcaatgtgcg tagtagtttc cttggtttta tggggtattg actcaatcat
    22861 cgtcgcatta attacatttg taacaagttt aaggttctaa aatgagtgaa gtagaagtga
    22921 aagaaaatgg ctcaaaacgc tggtatgtat tacaagcgtt ttcaggcttt gaaaaccgtg
    22981 tggcagtaac attgcgtgaa tacattaagc ttcacaaaat ggaagagcag tttggtgaag
    23041 tattagtgcc aaccgaagaa gtcgttgaaa atgtgggcgg taaacgccgt aaaaccgagc
    23101 gtaaattctt cccaggttat gtattggttg aaatggaaat gaatgacgat acttggcatt
    23161 tagtgaaaag tgtgcctcgt gtaatgggct ttattggtgg tacggcagat cgccccgcac
    23221 caattaccaa acgtgaagca gaccgcattc taaaccgtgt acaagaaaca gcggaaaaac
    23281 cacgccacag aaaagaattc caaccgggcg aaagcatttt ggttacagaa ggtccgtttg
    23341 cagatttctc cggtacggtc gaagaagtgg attacgaaaa aggtcgctta aaagtatcgg
    23401 tgtcgatttt tggtcgtgcg acaccggtag aattagaatt cagtcaagtg gaaaagcaga
    23461 gctaatttca attaatttca cacacaagcg gttatttttc tcaattttgt gcaaaattca
    23521 gcggaaaaaa gaccgcttgt aacaagattt attcaacaaa gcgaaagttt tgcttgaaaa
    23581 atagtcgcaa attctttaaa atttgcgacc tattacgagc cagcctgctg actcgtattt
    23641 tttatgtaac aggggagctg attttcagcg ttatcaccca tttagaggaa aattaaaatg
    23701 gcaaaaaaag tccaagccta cgttaaactg caagttgcag cgggtatggc taacccatca
    23761 ccaccggttg gtcctgcatt aggtcaacaa ggtgttaaca ttatggaatt ctgtaaagca
    23821 ttcaacgctc gtactgagag cttagagaaa ggtttaccga ttccggttgt tatcacagtt
    23881 tacgctgata aatctttcac attcgttacc aaaactccac cggcggcagt gttactgaaa
    23941 aaagcggtag gtatcaaatc tggttctggt aaaccgaaca aagataaagt gggtacagta
    24001 actcaagagc aaatccgtca aatcgctgaa actaaagcag cagatatgac tggtgcgact
    24061 atcgaaacca aaatgaaatc aatcgctggt acagctcgct caatgggctt aatcgtagag
    24121 gaataataca atggctaaat tgactaaaaa aatgaaagca attaaagctg gcgtagattc
    24181 tactaaagct tatgacatca acgaagcagt tgaagtgtta aaaaaattcg caaccgctaa
    24241 attcgttgag agcgtggacg ttgctgtaaa cttaggtatc gattctcgta aatcagacca
    24301 aaacgtgcgt ggtgcaaccg tattaccaca cggtacaggc cgtaccgctc gtgttgctgt
    24361 gtttacacaa ggtgcaaacg cagaagcagc aaaagcagcg ggtgctgact tagtgggtat
    24421 ggaagatctt gcagagcaaa tcaagaaagg cgaaatgaac tttgatgttg ttatcgcttc
    24481 tccagatgca atgcgtgttg taggtcaatt aggtcaagta ttaggtccac gcggcttaat
    24541 gccaaacccg aaagtaggta cagtaacacc aaacgttgca gaagcggtta aaaatgcgaa
    24601 atctggtcag atccgctacc gtaacgacaa aaacggtatt atccacacta ctatcggtaa
    24661 agcagacttc tcagctgaac aattaaaaga taacttaaac gcattgttag ctgctttaac
    24721 aaaagcaaaa ccaacaacag caaaaggtat cttcatcaag aaagtaagca tctctactac
    24781 aatgggtgct ggtgttgcgg ttgatcaagc atcactttaa tttttaatag aatcaaaaga
    24841 ccgtagtgtt aatactctac ggtctttttc tttaggtacg aatgttaaga ttattatctt
    24901 tttgctttat ttttttgctt agtgcttgta ctgtattacc tcaaattaag caattaccgc
    24961 cacaggttga atctgtaaaa tcctttaaag ttgaaaataa tggccagcaa agtttgcttc
    25021 taattcaatt ttatgaaaat aaatggcgtt ggattcaaac tgatccttta ggtgctccta
    25081 ttgccagagt atggcttact aaaaaaggtt gggagccaga tggctttgta atgccaaata
    25141 agcaggcaca gctattattt acagctttag tcaatgcatt aaaccctcat tctccacctt
    25201 tttatttaga taaaggttgg aaaattgaga gggaaaaatc ccaatttacg atttggctac
    25261 cagatagcag taaatggaaa gtggaaatat tgggggaata agcgaaaact tgaacctcta
    25321 tgcttgaatc acttgaatat gaattttata attttagtgc cattacacaa taatactgga
    25381 ataaataata atgacactat atttatcaga gcccgccatc accagtccga ttggatatgg
    25441 catcgtggag catttgaatg ttttaattaa tgcgaaagta tctcctttag atacgacttc
    25501 acagccttat ttacgacata atatttcaaa tagttcaccg aaatttctag ggcaagtctc
    25561 tgctagattg cgtgattttc ctgttgaact atctattgaa caccgcagcc gcaataacca
    25621 actgctatgg cacgcccttg cagaaattga gccgaaaatt accgcttgta tcgagcaatt
    25681 cgggcgtgag cggattgcgg tgatcatcgg cacgtccact accggcgccg atgaaaatat
    25741 tcccgtcttc aaagccggtg tgcagggcga ttatgccacc gagcagttta atcaacagca
    25801 aattctcttc tctgcgccgt ctgatttcgt tgccgaagtg tatggcttaa cggggctgag
    25861 ttacggcatt tccaccgcct gcacctcagg ggcaagagcg ttaatttctg ctgcccgttt
    25921 actgaaagcc ggcttgtgcg atgcggtgat ttgcggtggt gtggataccc tttcgccttt
    25981 aactatcagc ggatttagct cgctggaagt gttatcaaac caacaaacca acccattttc
    26041 cgctaaccgt aacggcatta acatcggcga aggcgcagcg gtgtttgtga tgagccgtga
    26101 accactggac gaacatcgca ttcagttact gggttatggt tccagctcgg acgcttacca
    26161 tatgtcctca cctcaccccg aaggggaagg ggcaatttcc gctttccaaa atgccctgca
    26221 caatgctgat ttagacgcag ccgacatcgg ctggatcaac ctgcacggca cagggacgct
    26281 tcataacgat caaatggaaa gcctcgctgt ggcaaaaatc ttcggtaatc agaccgcttg
    26341 cactaccacc aaaccctaca ccgggcatac ccttggggct gccggtgcgg tggaagcggc
    26401 aattttgtgg ggaatgctct gtcgccaaca caacccgacc ggcaagctcc ctgcgcaatt
    26461 atgggataaa caagcggacg aaaacctgcc gaacattgcg atcacctatg aacagagccg
    26521 ttgggcagca ggcagacgga ttggcgcaag cagctctttt gcctttggcg gcaataacag
    26581 cgtactgatt ttgggagaaa gcgatgcata aattcacctt ccctatcgaa caaattgcac
    26641 cattggttcc tcacagtggc aatatgatcc tactcgaccg cattaccgca ttcggtgagg
    26701 attttttgac cgctgaaacc aatattcgcc ctgacaatcc gctgatcaaa cacggaaaac
    26761 ttgccaccta cgccagcatt gaaattatgg cacaaggcgt ggcggcgtgg gctggctgta
    26821 ttgccacaca agccggcgaa ccagttcgct taggctacct gctcggcact cgcaaactgc
    26881 acctccaccg ccaagaaatc ccgattggct cacagctaca aattcagatc aaaatgtcta
    26941 ttcaagatgc caccggcttc ggcgtattcg actgccaact gatcgattta gccgacaacc
    27001 aagtgctttt agaaggggcg ctgaatgtgt ttagtccgaa atagagttgg tagtagtaag
    27061 tagtagtaag tagtagttgg tagtagttgg tagtagttgg tagtagttgg tagtagttgg
    27121 taggaaattc gttatgcaag aggcctatga cattcaacat ctttgtactc gcaaagaaga
    27181 taaatccttt gatcgcaaaa gtgcgagaaa agaaccgaaa gagattttgc ggcatttgat
    27241 tggttttgcg aatgcagacg gcggtttatt agttattggt attgaagata atggcgaaat
    27301 taccggtttt aactaccaaa aagcccatca gccggaagaa tttatccacg ctctgcacaa
    27361 tttacaaaaa atgccgatac cggtgaccta tcagcaaatt gaggtggtaa atagtcgagg
    27421 tttagcggat tttgtcttat tatttgaagt tacagcttcg agtgggaggg cgattacctc
    27481 tcacgatgat cgggtttttc tacgctcaaa agatcaaact ttggaactca attttgaaca
    27541 acgtttgcaa ttagagtacg aaaaagggca gcgtgctttt gaagagcaac cgatagcacg
    27601 cgctgatttt actgatctgg atatggattt attagaacag tatcgacaga aattacacac
    27661 agatcgctca atagaagaga ttctgaaagc ccgagattta attaacaaag aagggcaaat
    27721 tacgaatgcc tgtattttgc tatttgggaa taacccgaca cgttattttc ctaatgcccg
    27781 tttgcgtttt ttgcggtatg aaggtaaagc acctctgacc ggtagaactt ttaatgtcgt
    27841 caaagaattt atgcttgaag agcctattcc taagttattg gagagtgcta aggcagtatt
    27901 aaaaagccaa ttacgagagt tccaacgttt agatgaaaac gggcaattca caaaaattga
    27961 tgagtatcca gaatttgctt ggttagaagg tattgtcaat gcgattgcac accgtagtta
    28021 cagtaatcaa ggggactgca ttcgcatttc gatgtttgat gaccgtttgg aaatttttag
    28081 tccgggtgga ttacctcgcc ctgtgacgct tgaaaatatg tactataccc gtttttcacg
    28141 caatccgaag attgcacgag tgctgtatga atttggttgg gtcaaagagc tgaatgaagg
    28201 tgttaatcgt atggcggagg aaatgaacgc ttaccaactt aatacacctt cttactttga
    28261 acccaatgaa aatgcggtca gactgatttt agaaaataat attgaacatc ggacattaag
    28321 ggcattagaa cgaatagaag aaatttttac gcaggatatt atggcgacat taaataaaaa
    28381 tgaagttact atcctgcaat atttatatga aaatgaaatg attactgtaa agattgctag
    28441 tgagatatta gatcggagta aagaattatc tcgtagaata ttaaaaagtt tagaacaaaa
    28501 aaatattctc acttggcacg gcttaagtcc aaaagatcca acccaatact acacactcaa
    28561 gaaatagtag gaaatataag atgaataata cagttctcat caccggctca agccgtggta
    28621 tcggtaaagc gattgcttta acccttgcac aacaagggtt tgatattgtg gtacattgcc
    28681 gttctcgtgt ggaagaagcg gaagcggtgg cacaagcggt cagaaatctc ggaaaaaatg
    28741 ccagagtgtt acagtttgat gtggcggatc gtgaaaaagt gcggtcgatt ttagaggcag
    28801 atgtggaaca acatggtgcc tattacggcg tggtgctaaa tgcagggctt acccgtgata
    28861 acgcctttcc tgctttaacc gatgaggatt gggatagcgt gttgcgtacc aatttagacg
    28921 gtttttataa tgtcctgcac ccgattatga tgccgatgat ccgtcgccgt aaagcaggca
    28981 ggattgtctg tatcacctcg gtttccggct tgatcggcaa ccgtgggcag gtcaattaca
    29041 gcgcttcaaa agccggcatt attggtgcgg caaaagcact cgccatcgag cttgccaaac
    29101 gtaaaatcac ggtaaactgc gtggcgcccg gcttgattga taccgatatt ctggacgaaa
    29161 acgtcccaat cgaagaaatt ctcaaaatga tccccgcagg acgaatgggc gatcctgatg
    29221 aagtggcaca cgccgttgcg tttttaatgg atgaaaaagc cgcttacatc acacgccaag
    29281 tcatcgctgt gaatggtggg ttgtgttaac tttaggataa taattcgtag gggcggatcc
    29341 agtacccgcc cgaagccaaa aagagataaa tatgaaacga gtcgtcatca ccggaattgg
    29401 tgcggttacc gcttttggta aagagtgggc ggaaattcac ggctatcaac cgccgaaaca
    29461 ttggaaccgc aagcagttgc gcagccttgg gcgagtgtcg caatttgtgg tggaggccag
    29521 cgagcgggct ttagcacagg ccggtttgtt ggacgaaaac ggcgtgattt tcgactggct
    29581 aaaagacggg cgaatgggcg tggcgtgtgg ctcgtccaca ggctctacca atgacatcaa
    29641 agatgccgcc gaattgctga tgaccggcaa atcggacggt ttcaatgcca acacctatgt
    29701 gcgaatgatg ccccacacca ccgctgccaa tattggcatt ttcttcgggc tgacagggcg
    29761 gattatcccg acttccagcg cttgctcctc gggcagccaa ggtatcggtt acgcttacga
    29821 agccattaaa tacggtatga tcccgatgat gcttgccggc ggtggagaag aattttgtgc
    29881 ctccgaagtc tatgtcttcg acagcctcta tgctgccagc cgccgcaacg acaacccaaa
    29941 aggcacgcca cgcccctatg acaaagaccg agacggctta gtgatcggcg agggagcggg
    30001 aatgttcgta ttggaagagc tggaacacgc gttagcacga ggggcaaaca ttatcgccga
    30061 agtggtgggc tacggtgcaa acagtgacgg ttcccacgtc acccgcccac aagccaccac
    30121 aatgcaacgc tgtatggaac ttgcccttaa agatgccggt attcaaccga accaagtcgg
    30181 ctatgtaaac ggacacggca cggccaccga gcagggcgac attgccgaaa ctcaagccac
    30241 cacagcggtg ttcggcaaaa ttccgctcag ctcgcaaaaa agctacttag ggcatacgct
    30301 cggtgcgtgt ggtgcattgg aaagctggtt cagcattgaa atgatgcgag acggctggtt
    30361 cgccccaaca ctgaatttag aaaatgtcga tgaacgctgt ggcgacttgg attaccttca
    30421 aggtgacgga cgggaaatcc acaccgatta tgtgatgaac aacaatttcg ccttcggcgg
    30481 tatgaatacc tcattgattt ttaaacgttg gaaataaccg ttttcaaata atgaaaaaga
    30541 tattattagt ttattactcc caaaccgggc aactcacccg tttggcagaa aattttgtac
    30601 aaccgcttga acaagcgggc gtttttgtgg aaaaactcgc aatcaaaccg caacaggaat
    30661 acccgtttcc gtggcggttt atgcgttttt ttaacacctt ccccgaaacc gtccacctca
    30721 caccgccgcc gattgagcct ctgccctttc agcacgaaat atacgatttg gtgatcatcg
    30781 cctattccgt gtggtttctc tcccccagcc agccgattac cgctttttta cagagtgaac
    30841 aggcaaaaaa agtgttgaag gatacgcccg tcatcacgct gatcggctgt cgcaatatgt
    30901 ggctgatggc gcaggaaaaa gtgaaaaaac tgcttgccga gagtgaggcg aagctcattg
    30961 gcaacgtggt caaagtggat caatctaacg actgggcgag ttttatcacc acaccgcttt
    31021 ggatgctgac cggcaagaaa aaaatcggca agctgccgag cgctggcatt gccgaaagcg
    31081 aaatggaaga tgtaaaacgc ttcggggcaa aacttttgca catttttagc caaaaccaac
    31141 cgcttgacag cacccttttc cgccaaatgc aagcggtcaa aattgacgaa aaattgatga
    31201 tgagcgaaaa agtcggtgcg agaagtttcc acctttgggg caaattactg atcaagtgcg
    31261 gtcaaatttc accgcactta cgccaagcgg tactctattt ttacatcctc tttttagtga
    31321 cgttaatttt aaccgtcgtc ccgatttcaa cggtgattaa acggctatta cgcccactgc
    31381 tgaaagaaaa attagaaaaa caaaaacggt attttgccga gccttcgggc gagtaaacgt
    31441 accctacacg gaaactttat gctatctaac gtctatatca ccaacacctc cgccttttta
    31501 cccaacgccc ctgtgtccaa cgatgcaatg gaagcggtgt tgggaatggt gggggaaacg
    31561 ccttcccgag tgcggaagat gattttgcgt tctaacggta ttcaatatcg ttattatgcc
    31621 attgacccga taacccgcca agcgactcac agcagtaccg agttggcggt gcaagcggtc
    31681 aaaaaattgg gaaattctgc aacatcagcg acttgcttgg cggtggggac atcttacccc
    31741 gaccaaatta tgccggggca gggggtgatg gtacacggct tgcttgatgc cgaaccgatg
    31801 gaagtgctga ccactgccgg tgtgtgcgtg gcgggaatgg cagcgatgaa gcacgcctat
    31861 aatgccgtac gcatcggtga gcatcgtgct gcgattgcaa cggcttccga aaccgcctcc
    31921 gccattatgc gcagtgaaca tttccgtgcc gaaatcgatc ataaaaaact ggacgatgct
    31981 cgccctgaga tcggttttga gaaagatttc ctgcgttgga tgctctcaga cggtgcaggg
    32041 gcggtattgc ttgccaatca gccgaacgaa aaagggttga gcctcaaaat tcactggatc
    32101 gatctggtct cttacgctaa cgaaatgccg gtttgtatgt acgccggtgc ggaatttatt
    32161 gacggcaact acaccggctg gaaagcaatg agcgaagccc aacgtgccga atgctcggca
    32221 atggcggtga aacaagatgt caaactgctc aacgaaaaca tcgtcaaata cacggtggaa
    32281 aaagcattgg agcggattat cccgaaatac aagctcaacg ctgaagagat tgactacttc
    32341 ctgccgcact actcgtccgg ttccttccga gataaattat tggaaggttt acagcggatc
    32401 aatttcctga tcccacagga aaaatggttc accaacctga ctaccaaagg caacactggg
    32461 tcagcctcga tctacattat gctcgatgaa tttatccgca cccacgatct caaacaggga
    32521 caaaaaatcc tctgctatgt gccggaaagc gggcgttttt cgtcttgttt tatgcttttg
    32581 gaagcggtgg taaattcgta aaatcgtagg gacgggggct attcccgtcc aacaatcatc
    32641 ggaaaacgta tggacatcaa tgacattccc caagacaaca gcaaaatctt caacggcgaa
    32701 cgcaaagtga tctacgcccg caatccgcaa ccagaaatcc gccaaatgat ctcctctatt
    32761 ctcgcttgct acgcttggga cgaaaacaac ccgtttgtag cgaagtctga tgcacggttg
    32821 aaggcattgg cggaagttgt tggtaaagca gaaattggcg attaagtttg attttgtgct
    32881 ttacagggtc gcagattatc tgtataattt gcgaccttaa cttgcgagag caagctgatg
    32941 gtgcttagcc tatctaagcc ccgtctaaga ctgtaggggt gaaaacttaa taatcctaca
    33001 tagatggtga acagacagaa ttctctgctt ctggacacct taggctcaaa gaagattgca
    33061 gtttgtagca atgcaagcgg taatattttt gggatttttt gtaaattctg cttgaaaagg
    33121 cagagtgtat caggagctaa aaccaatggc attaaatctt caagacaaac aagcgattgt
    33181 tgctgaagta aacgaagctg ccaaaggtgc actttcagct gttgttgcgg attctcgcgg
    33241 tgtaacagtt gaaaaaatga ctgagttacg taaagcagct cgtgaagctg gtgtgacaat
    33301 gcaagttgta cgtaatactt tattacgtcg tgcagtggaa ggcaccgagt tcgagtgctt
    33361 aacagatacg tttaccggtc cgactcttat cgcattctca aatgaacacc caggtgcagc
    33421 agcacgttta ttcactgaat ttgcgaaaac aaacaaagag tttgaactta aaggtgcagc
    33481 ctttgaaggt aaaaaacaag atgttgcatt cttagcaacc ttaccaactt acgaagaagc
    33541 aattgcacgt ttaatgggca caatgaaaga agctgcggca ggcaaacttg ttcgcactct
    33601 tgcagcatta cgcgatcaaa tggaagcagc agcttaattt taagcactct tctttattcg
    33661 tttaacaact tattttactt taggaatcga ttgttatgtc attaactaac gaacaaatca
    33721 ttgaagcgat tgcttctaaa tcagtatcag aaatcgttga attaatcact gcgatggaag
    33781 aaaaattcgg tgtttcagca gcggcagcag tagcagcagc tccagcagca ggcgcagcgg
    33841 cagcagaaga gaaaactgag tttgatgtag tattagcaga agctggtgca aacaaagttg
    33901 ctgtaatcaa agcagtacgt ggtgcaacag gtttaggctt aaaagaagct aaagacttag
    33961 tagaatcagc tccagctgca ttaaaagaag gcatctctaa agcagaagct gaagcactta
    34021 agaaagaatt agaagaagct ggcgcgaaag tagaaatcaa ataatttttg atttattttg
    34081 tcagaacttc aagttcaaca aaccccgatg gaaacatcgg ggttttgttt tttcagaatt
    34141 gtgtaatgta gatatgatta cctttatcca ttacttgcgc agcagtgaaa aatgtaaaaa
    34201 ttccacaaag tgatctgccc agtgcatttc gtggtggatt tcatttgcca taatccgcag
    34261 gcggatattg tctaacggca cgccaatccg aatcagtgct tggtagtaat tgagcgaaca
    34321 atcaatatag gtttggttcg tattagagat aaagtgcgaa tcgatttcat cgccttcgtt
    34381 agtgccaacc tgaataaaca ctttggtatt gggttgtaac ggatattggt gcgtaaaacg
    34441 cagaaaatcc ggctcactaa accaagaggc aagcgaaaac acccctaaat gcccgaaaat
    34501 atgtgggtag cggctgccca tataagcggt gataatgcca cccattgaac tgcctgccaa
    34561 taaggtatgt tcttgctgag gcttggtgcg atagtgaata tcaataaagg gtttaaccgt
    34621 ttccaccacc catttggcat attcctcccc taagcctccg gcatttcggg tttcaggggt
    34681 tgagccggtg gttgttttcc acgggccgta ttcatttaag cgattagctc ctgcgttatc
    34741 aatgccgaca ataatcatct tcggtaattc ttggtgtgat ttaatggttg gaataatttt
    34801 ccaagaatgc cccgaataag actctttgct gtaaaacaca ttttgcccgt cgtgcatata
    34861 taacaccgga taacgttccc aaccttcgtt gtgataatcc ttcggcaata gtacccgaat
    34921 gcggcggtga tggtgtaaat agggaacata taaaaaatgc tcctgcattt ctaaatagtg
    34981 gttaagtggt ttttgtacca tcagtctgtc cttttgctta aattgtggcg tattttacac
    35041 cttaatcagt ctgagttaaa acaagcggtc agaaaaagtc aaaaatttgc aaattgctgt
    35101 tacctaacgc aatcggttgc cttatgcttt tgaggaatat aaaagagaaa ctgtgatcta
    35161 agtcacagca atttttattc ggtttgacta aaatccgaac tatcacttaa tagagaggaa
    35221 aatgttatgc aggcttcatt acaagatatt cttgaggtag taaaagcaaa aaatctcact
    35281 tatcagcaaa aattgatgag tttaggcaat attgctgagc ggttatttaa cccgattgaa
    35341 ctacttggct acactgaaga agagtgggaa cacattgaaa atcagatgat ttgcgattta
    35401 aatgaaggtt atgcgattta tcgcccacgt tatattttgc ctgattataa tgtgtatatg
    35461 caaaaaggtt gccaattctt agatttacct ccgccaacca atttagatga agccttagac
    35521 ggcttgttaa ttctgtattc acacgttccg tccattacta cttttccggt gtatatcggg
    35581 cgtttagacc aactgttaga accgtttata accgatgaag agcaagatta cattaaaatc
    35641 aagcgtttct taaatcatat cgataaaacc atttccgatt ctttctgcca cgctaacatc
    35701 gggccgaatg acaccaaagc cggtcgttta atcttaaaag cggtgattga gcttgaaaac
    35761 actacaccga atatgacgat tcgttatgat aaaaccaaaa catcgcgtga atttgccgaa
    35821 ctggcggcga aagcgtgctt gttggtgtcc aaaccgtctt ttgctaatga tgcctactac
    35881 attcaagatt taggcgaaga atacggaatt gcaagctgtt ataacgcctt accggaatgt
    35941 ggcggtgctt acactctgac ccgcctgcgt ttaggtacta tcgggcgtgc ttgtgcgagt
    36001 gttgaacaaa tggtcaatga gatgctgccg aaagtggcaa aactcgccct ttccacgatg
    36061 gataaacggc ataaattctt ggtggaagaa agcaatttct ttgaaagtag cttcttggtt
    36121 caggagggct ttatcaaacg tactaacttc acaagtatga ttgcgattgt aggtcttgcc
    36181 gatgcggtga atcatttact caagcaagaa ggtttaaatg aaacctttgg gcaaagcaaa
    36241 cgtggcgatg aaattgcgac gctgattatg gataagctcc aagcagtaaa tgacgctcat
    36301 aaagggctgt atgttgaacg aaccaataac caatatctgt tacacgctca agtgggggca
    36361 aataaccacg ctgaagacaa aatgaacacg ccggcacacc gcattcgtgt gggcgaagag
    36421 ccaaccttat tggcacactt aaaacagtct gctcctttcc ataaatattt cccatccggt
    36481 acgggggatt tattcgcctt tgaccaaacc tacactgatc atttagatgc ggtggtggat
    36541 attattgacg gctcgttcgc ccaaggttat cgctacatta ccacttacct gaaaaatact
    36601 gatttgattc gagtgacagg ctacttggtg aaaaaaagcg aggttgaacg ttatcgcaaa
    36661 ggcgaagcgg tattgcgtga taccacttgg tatggttcag gcaccgatga ttgtgcgaaa
    36721 gtgttcgacc gccagctgcg tgatgaagaa aatgtgaatg cagggtaaca atggctgcac
    36781 tttctgagat ttttgtgccg ttacaccgca ttatcccctt ctcgaatgta gaagggcagg
    36841 gcaatcgtac cagcattttt ctacaaggtt gcaagctgaa ttgcctgtat tgccataacc
    36901 cggaaaccat tccacgttat agcaaggaag caaaacaggt tagcttgcaa tacttgtacg
    36961 atcaagtgat ggaggcagtt ccctttattc gaggagtcac tatttcgggt ggagagccaa
    37021 ccattcatca taaaaaatta gtgccactgt tcgaggcgtt gcgtgagcaa gggctaacct
    37081 gttatttaga cagcagcggc ttttttgatt ttgaggcaac agagccgtta attaatgtta
    37141 ccgataaatt tctgttcgat ctcaaaggag atggggcggg cttacaaacg ctatgctttg
    37201 atcggaaaaa ccaagccggc aaagtgcctg aacaggctct tccaaacgtg aatcatatta
    37261 aacctgaaaa cttggctcgc aatttgcaaa atttagccaa attattaccg cttgataagg
    37321 tggaagaagt acgtttggtt tacctcaatg agttttataa cgcccaccat ttgctcgaaa
    37381 aagtggctga cctacttaaa ccttacccag atgtactact taaaattatt cgggtacaca
    37441 ccaaaggagc gagagatgaa acaggcttgg ctcgttttgt acccactatc gagcaaaccg
    37501 atgaactggc agcatttgca aaacggcacg gtattcaaaa agttattact atttactaat
    37561 ggagacttac gatggatata ctaattagca taattggtgt ttttgtgtta ttaggcttag
    37621 gtgttttatt atcaaataat cgcaaagcca ttaaatttcg cacaattttg ggcgctttgg
    37681 cgattcaaat tggttttgcc gctcttattc tttactttcc agcgggaaga aatgcattgc
    37741 tagctactgc aaattgtgtg agcaatatta ttaattatgg taacgaaggt attagctttg
    37801 tgtttggcaa tttagcgaat ccgagtaata gcagcattgg ttttgtgttt gcggttaaag
    37861 tattgcctat tattatcttc ttctctgcgt taatttcaat gctttactac cttggtgtga
    37921 tgcaatgggt gattaaaatt attggtggag cattacaaaa aacactaggc acttccaagg
    37981 cagaatcaat gtcagcggca gctaattatt tttgttggtc agacagaagc tcctctagtt
    38041 gtaaaaccat acattagccg tatgacagag tcagaattat ttgccattat ggttggtgga
    38101 actgcttcaa ttgctggttc cgttatggct ggttatgccg gaatgggcgt tcctttaact
    38161 tatcttattg cggcctcctt tatggctgca ccagctgggt tactatttgc taaattaatg
    38221 tatcctcaaa cggaaacctt ggagagtaaa gcagatgaca atgttgaagt tgaaaagcca
    38281 aataacgtgg ttgaggccct agctaatggt gctagtgcag gtgttacttt ggctattaat
    38341 gtcggtgcta tgttaatagc atttattgca gtgatcgctt tattaaatgg ctttattggt
    38401 ggcattggta attggtttgg ttatgagggg ctaaccttac aaattttatt aggttattta
    38461 ttccaaccac ttgctttctt aatcggtgtg ccgtggcaag atgccgatat tgcaggtcaa
    38521 atgataggta tgaaacttgc tgttaatgaa tttgtcggat atcttgagtt tgctaaatat
    38581 ctacagcctg atgctgtggt gcaattaagc gataaaacta aggcaataat tacttttgct
    38641 ttatgtggtt ttgcaaactt tagtgcaatt gcggtattga ttagtggtat cggaagtatg
    38701 gcaccaagtc ggcgttcaga tattgctcgt ttaggtttaa aagcagtgat tggcggcaca
    38761 ttggcgaact taatgagtgc aactatagcg ggtttcttta ttggtttaag tggtgccgca
    38821 ttaggttgat acaagcggtt aaattgactc acttttttgc aaaaagccca agtaagttaa
    38881 atgactactt gggctttgtt taatgaacta gctcttagtt tcggtttttg ctgcgttatc
    38941 cttttccgct ttggctgtct gtgttttcga agttgtctgt ttttttgcgg attggctagc
    39001 agtagctgct tttcttactt tctttgtttt aacttctact tccgcatttt tagggttttt
    39061 atccgctaac catgcctgca ctttagctaa gaatatttgt cccatcgggc gaggatcgcc
    39121 agaaaataat gtttgtaatt tattattttc aataatgtcg cgtctaattg ctaagcgttc
    39181 ttcgtggttt tccgctaaac gaatagcttg agtaacgtat tcctctgctg tttgggtaat
    39241 taaccaatta ggtagcccga ggcgcttaaa taaaccttca tcaatatgtt catgaacttc
    39301 agggcctgtt ttacagacac caactaaacc aagcgtaacc atatcgataa tgccgtttgt
    39361 atttccaaat gggaatggat ttagcatcat atcgcaatta tgtagaatat ttagatattc
    39421 atcatatggc gaatgtgggt gtgcggttgc atcatttcct aaatagcttt tgataaaacg
    39481 ttccacataa ggatgcgtaa ttccactaga ttgccctaat gcaaaatgga aatgagtttt
    39541 taccttcgcc ctatcacgaa tggcttttaa tgcctctaag aaatatgggt ttaatttcat
    39601 agtggttgat gcaattccga tatggataac ctctggattt tcacgtaggt tatagaccac
    39661 attttgaggc gctaatgcgg aaggcacata gggtagcgca tcttttggaa gacgtaataa
    39721 ttgttcacta aagcagcttt cggaaccaac atagtcatct tctacgatga catattcaat
    39781 aaaatcagag tgtgtagttg ccggatgacc tagcgcaaca gcttgaattg gtgctaggcg
    39841 agtgttacta gcgaaaatag tggttaaatc cattccaatg ctaggcatat agaatacggc
    39901 tggattattt ttatcacaga tatcttttaa aaagcttaag cggtcaaata gtgagccatc
    39961 ttgtggtaat aaatgaaatt cgtcgaatac ctgttgcccc atttcatcta cagcatcact
    40021 gcctagccca ataagatgga aatgttcgcg tgcagcaacc attgaggttg aatgagtacg
    40081 ataaatagaa tgtgatgaat ggaaatgctc caataatact accatgacag gtttattatt
    40141 tctggtacca agctgttcaa tttttctatc ttcccaaccg acagaaagta aatgacgacg
    40201 aattacagca tttaacgacc gttttacatt atgtttattt gcttcaatat cataactgca
    40261 atgcatataa acatcgtgtg aaatggcgct cggtagcttg ttcaagtttt caatttgcgc
    40321 taatttttct gggaaccatt gcaaaatagc cgcgcgtttt gcaaaagcag aggatgtacc
    40381 aataaatcta ggtgattgga gtgcaaaaca aagtgatgcg gtgagatctg atgaagcatt
    40441 ccatgcagca tctagattca ataaaatatt agattccggt aagtataaaa tacagaactt
    40501 aattagtgct tgtaatgtgg catctaaatc aaccgtattt ggattagcag attctttatt
    40561 tctattgtaa gtacgcaaga tatggtctgc gtttacgtat ggagaactgg caaaaattaa
    40621 tgctatccag cgttgtaatg tcagaaaacg catagcaccg tgttcggaga tctctaatgc
    40681 aggatcttca aacaagcgag taatcgctac cgccattcga gtacagaaat agatagcttt
    40741 atcattttct aaattttcaa tctgctctgg catattcaat tcaatattgg aaatgccacc
    40801 aaaattacta tctaattttg aaaggataga cagtagctcg gtacaagccg attcataatc
    40861 ttttttcgcg accgcctgct caaaacggat gacgctaggc atattttctg ctgacataat
    40921 gtatccttag ttgtttaaaa ttccccacag atcatactca tctgcatgtg taatggttac
    40981 tgaaataatt tgccctactg tcactaattg gtttgaagga ttatctacat agactacacc
    41041 gtcaatttcc ggtgcatctg ccattgagcg accgataatg ccttcttcgt caatgtcatc
    41101 aataattact tgtacggttt tgccgatttt tgcttgtaag cgagcagcgg aaatttcttg
    41161 ttgtagttgc ataaaacggt ggaaacgttc ctcttttact tcttccggca cttgatccgg
    41221 catatcggta gcaacagcac cttccactgg gctgaattta aagcaaccaa cacgatctaa
    41281 ttgtgcttct tttaagaaat cgagtaatag ttggaaatct tcttctgttt cccccgggaa
    41341 gccgacaata aaggtagaac gcagagttaa ttcagggcaa atttcacgcc attttttaat
    41401 gcgttctaag gttctttcga ttgaacccgg gcgtttcatc gcttttaaga ttttcgggct
    41461 ggcgtgttgg agtggaatat ccaaatacgg caaaattttg ccggcagcca taagcggaat
    41521 taaatcatct acgtgtgggt atgggtaaac atagtgcaaa cgcacccaca cgcccattga
    41581 gcctaattgc tcacatagcg taattaaatt acttttaatc ggcataccgt tccagaaaac
    41641 ggttttggtt tcttcttctt ttttgcgatc taacgaatag gctgaggtgt cttgtgagac
    41701 gattaacagc tcttttaccc cggcatcaac taggcgtttt gcttcatcta atacttgcac
    41761 gatagaacgg cttgctaaat cgccacgcat tgaaggaata atgcagaaag tgcagcgatg
    41821 atcgcagcct tcggaaattt tcaaataagc gtaatgcttc ggtgttaatt taatcccttg
    41881 ttttggcact aagctggtgt aagggttaaa ttccggtttt ggcacatatt tatggacgtg
    41941 gttcattacc gcctcatagc tatgtggccc tgtaatttcc aatactttag ggtgtacctc
    42001 acgaatttgg ttttcttttg cccctaaaca gccggtgaca attacttttc cgttagcttc
    42061 taacgcttca ccaatgcttt caagagattc ttgtactgcg gaatcaataa aaccgcaggt
    42121 gtttacaatc acaagatccg cattttcata actagggatg atgttataac catccgcacg
    42181 cagttcggtc aaaatacgtt cggaatcaac taaattttta gggcagccta agctgataaa
    42241 accaatattt ggtgaagcac tcatataact ctcaataact aaaaagaaaa atgcctacaa
    42301 tttttgtctg taggcaaaaa actaacaaga ttctacaaaa tcttgcccaa aatagcgata
    42361 acaagcggtc aatttttaat aaacatttac aaacttgaaa tttcgctaac aattaaaatt
    42421 aatcgatatt atttagcatt gctaatacaa tcgtggaaga tttttctgcg gctaatggca
    42481 gaaattcttc aaaagaaagg tgggattctt tgtccgctac atccgatact gctcttacga
    42541 ttacaaatgg aaggttaaaa ccgtgacaaa cctgcgcaat ggctgctgct tccatttcga
    42601 ctgcaacgac attcgggaaa ttctgacgaa tttgattaat cttatcatta ccgttaataa
    42661 aggcatcgcc gctacagatt aagcctgaaa ccacattgta accggctttt ttcgcttcct
    42721 tttgagctaa ttcgactaat tgctcgtttg gcgaaaatgt tggcgggttt gccggtagtt
    42781 gcccgatttc ataaccaaag gcggtcacat caacatcgtg ataacgaact tcggttgaaa
    42841 tcaaaatatc acctacgttt aaatctggat ctaatccacc ggcagagcct gtattaataa
    42901 tcatatccgg ctgggtgagc tgtatcagta aggtagtgcc gatggcggca gcaactttgc
    42961 cgatacccga ttgtaataat gccacacggg tattgttaat tttgccttca aaaattttac
    43021 aacctgctaa ttcagtgact ttaggttcaa ccattacttg gcgtaaaatc tcaatttctt
    43081 gtgccatcgc accaataata ccaattttca ttactttcct tctctaatgt attgtttatt
    43141 ttttgaaaaa aacgctattt taacagcatt aatagtgagg agggggtgtt tcttctgctt
    43201 gtgaagcaac ttggcttagt tgagcccctt tcaatttctc agcaatatgg cgaatctgag
    43261 tttgtaactt atcaattgca aattgttgct caataagtgc ttgatttaac tcttcaataa
    43321 tcagctcttg gaaagcaact ttagtctcca attcggtaat ttttgccgat aatgtataat
    43381 tattatccat tttttactaa aaaaaggttg tgtgaaaagg aattacactt taccctatct
    43441 taaattttct taacagttat ttttataagg aaaaattatg ttaaaaacca aattatcatt
    43501 atttactctt attgttggca cactttttag tacaaatgtg atggcagaag aaaaagcaga
    43561 tcctaaattt gtggatgatt cctcttatgc cgtgggtgta ttaatgggta aaaatattaa
    43621 tggtgtgatt gagtcacaaa aggatatttt tacctataac caggataaaa tcttagccgg
    43681 tgttcaggac accttaaaga aaaccggtaa attaaccgat gaagatttgc aaaagcagtt
    43741 aaaatcgtta gatgcctatt tatctgcgaa agaagcaaag attaacgaag aaaaaaccaa
    43801 agcaacggta gctgcaggcg ataaattccg tgctgattat aaaaaacagt ctggcgtaaa
    43861 agaaacggct tcaggcctgt tatataaaat tgaaaaagca ggtgaaggca attcaccaaa
    43921 agcggaagat actgtgaagg tgcattacaa aggcactttg actgatggca cggtatttga
    43981 cagctcttac gaccgtggtg agccgattga atttcagtta aatcaattaa ttccgggttg
    44041 gattgaagcg attcctatgt tgaaaaaagg cggtaagatg gaaattgtag tgccacctgc
    44101 attagcttat ggtgatcgtg ccgcaggcaa aattccagca aattcaacct tgaaatttga
    44161 aattgaatta ttagatttta aagcagcaga tgctaagaaa taattcgtta ttccttaatt
    44221 attgaagata cgtaagcggt tagatttatt ataatatttg caaaaaatgt gataaaacta
    44281 accgcttgta atttgtatta tgaccaaaaa attcacgcct ttttctgacg aagatcacgc
    44341 cattcttgct tcttactttc cgatggtaga tgggattgcg gcactgattg gtgagcattg
    44401 cgaaattgta ttgcattcgt tagagtttct ggaacattcg gcaatttata ttgctaacgg
    44461 acacaatact aaccgtaaaa tcggctctcc gattactgac cgtgcattga aatcgctgca
    44521 tcatatggca acggatagcg tctccaaacc ttattttacc aaggcaaaag gtggcgtttt
    44581 aatgaagtct atcactattg cgattcgtaa tggtaaacag cacgttatcg ggttactgtg
    44641 cattaatatt aatttggaag tacctacctc acagtttctc aattctttta ttgcccctgt
    44701 agataccgct tcagaagtta cttttgccag ttcagtagaa gatttggttt cacaaacgat
    44761 tcaaacgacc attgatgatg tgatggcaga tcgtcaaatt tctaacaata ataaaaatcg
    44821 tcaaattgtg atttcgttat ttgagaaagg gatttttgat attaaagatt ccattaacca
    44881 agtagcggag aagctggata tttcacgcca tactgtgtat ctctatattc gtcaaattaa
    44941 gcaggacaat gattaatgga ttatgtatta gcgataaaat caccggttta tggttcacaa
    45001 ggggcatttt tagcctacca agtggcggaa gaattattaa aagccaatca tacaatttgc
    45061 caaatattct tttttcaaga aggcgtgagc aatgctaatg ccttagtgaa tccagcaagc
    45121 gatgaagtga atttagtcga gatgtggaaa aaattggcta aattgcaccg cttgcctctg
    45181 catttgtgca ttgctgctgc ccagcgtaga ggcgtggtgg atgagcaaat ctcagctaat
    45241 gcaacacata atttagccga aggatttacc ctagccgggt taggcgaatt tagccaagca
    45301 gtattaaaag cggatcgatt actgactttt taagatgaag aaatataaac ttgccttttt
    45361 atttacccag ccaccttttg gttcggcagt tagccgtgag gggctagatg ccttgcttgc
    45421 agctagtgct ttttgtgacg agcaagagat agctatcctc tttatcaatg atggcgtatt
    45481 taatctcatt gccgatcaac agccggaaaa actcttacaa aaagatcata tttccacctt
    45541 caaattattg gagttgtatg acttaactga atgttttatt tgccaagaaa gtgtaagttt
    45601 acgagggctg gataatgcag attggttact gcgaatgctt caatttaaaa ctcacgctga
    45661 gatgatcgca ttgctcaatc aagctgaaaa agtaatgacg ttttaaggaa tatttatgct
    45721 ttataccctt tccaaagcga attatgatga agaaacctta aacttctttt tctcacatct
    45781 tagtgagaac gatgcggttg ttctgtggca agatggcgtg ctacaagcgg tcaaaagtcc
    45841 gcaatttttt gcaaaattgc cccgttgcta tttattggca caagatgtgc aagctcgagg
    45901 attgcaagag aagctcactg catttcagtg catttcatta tccgaattag tcaaattaac
    45961 agcggtttat tttccccagg ttgcactgta acctagagag gatatttttt gtaataccaa
    46021 ataatttaga ttatgatgtg attcctaaaa aggagaatat tatgaaaaca aaatgtctta
    46081 ttccgctact agcacttaca cctgttatgg tgatggcaga agaaaataac acttgggtag
    46141 acaagaaaca tgaaacagta agcactacct tagatcgttg ggcaaacaat attaacagtt
    46201 ggctaggtga tgtagaccca gataaaccgg gaacggcttc tttacgggta atgttagata
    46261 gccgttggga taaacacgat aaatttactt ataagccccg tgttcgtggt aaaatcaaac
    46321 tccctatctt gaaaaagcat ctaaatgtcg tatttggtga tgacgaactg gataatgagc
    46381 ttaccgataa aaatcatgct ggaaaattgt ataaaaacgt caatccaaat aaacgctata
    46441 actctcgtga agcgagagat tcgaactctt ctatcggttt acgttggtct gatggcatta
    46501 aggctttagg gattgagacc gatttagatg ctggtattcg ttcgggaagc gatattttcg
    46561 ggcgtttacg catcagtaaa gattggaaaa tcaccgacca gttcagtacc cgcctagagc
    46621 aaatttatcg ttacggttca gagagcaagc actatttacg tactaacttt gaaaataaat
    46681 ataccgaaac ggataccacc tttattatga accacaccaa ttttgaatat cgtcatgatg
    46741 tagatgaaga aagaagttgg gggaatagtt tatatcgtca acataacttc aaaccactca
    46801 cgcacatcag ctatggtatc agtatggggg ggcgtttaga taaagagtta tcaaaattta
    46861 actattgggg tccatttgtc tcctatcgtc aaccaatctg gcgcaactgg ctctttatcc
    46921 agcctgaaat cagtttttat aacgacaaag ataaagatcg taagcacttt attcagacat
    46981 ttgtacgttt agaggctatc ttctagctat taagcctcaa gtagaatctt ggggcttgtt
    47041 tttgcaagcg gtcaatttct tgccaaaatt tacaaaatcc gctataattc ctaaccattt
    47101 ttagcaaaaa ttggctgacg gcttgaccgg tcagcctttt gtgcttgaac gcaagggctg
    47161 attttgtcat tttaatcagc aaaatttgca aatttcctgc ccaatttgac cgcttgcagt
    47221 agcgatttct gactgcttga ttggcgattg gaaaatgaca tctcgtacag tttttataat
    47281 gccacttcca accgagtctt tcgtgtcgga ttgtgtaatc tcaaccaaaa tatatttcag
    47341 aggctatctt aaaaaatggc atattcatat tccgagaaaa agcgtatccg taagagcttt
    47401 ggcaaacgcc cacaagttct gaacgtacct tatctattaa caattcagct tgattctttt
    47461 gaaaaattta tcgaaaaaga tcctgaaggg cagcaaggtt tagaagcggc attccgttct
    47521 gttttcccga ttgtgagcaa caatggtgca acagaattac aatacgtttc ttacgaatta
    47581 ggcgagccgg tgtttgacgt gcgtgagtgc caaattcgtg gtacaaccta cgctgcacca
    47641 ttacgcgtga aattgcgttt agtctcttac gatcgtgaag ccgcagccgg tacggtaaaa
    47701 gacattaaag agcaaaacgt gtatatgggc gaaatcccat taatgaccga taacggtact
    47761 ttcgtgatta acggcaccga gcgtgttatc gtttctcaat tacaccgtag cccaggtgtg
    47821 ttcttcgatt ctgacaaagg taaaacccac tcttcgggta aagtgcttta taacgcacgc
    47881 attatccctt accgtggttc ttggttagat ttcgagtttg atccgaaaga taacttattt
    47941 gcccgtatcg accgccgtcg taaattgccg gcaaccatta ttctacgtgc attaggctac
    48001 accaccgaag aaatcttaaa tttattcttc gagaaaatta aatttgaaat tgccgataat
    48061 aaattattga tgacgttagt gcctgagcgt ttaagaggcg agactgcatc atttgatatt
    48121 gcagcaaacg gcaaagtata tgttgaaatg ggtcgccgta ttaccgctcg tcatatccgt
    48181 gcgttagaaa aagacaatgt gacccaaatt gaagtgccgg tggaatatat tgtcggcaaa
    48241 gtagcggctc aagattacgt tgatttatct tcaggtgaag tgatttgccc ggcgaatatg
    48301 gaaatctcga tggagatgtt ggcgaattta tcacaagccg gttacaacca gatcgaagta
    48361 ttatttacca acgatttaga ccacggtcca tatatttctg aaaccttacg tgttgatcca
    48421 acttacgacc gtttaagtgc gttggttgaa atctatcgta tgatgcgtcc gggcgaacca
    48481 ccaacgaaag aagcggcaga agcattattc gataatatgt tcttctcggc agagcgttat
    48541 gatttatcgg cagtaggtcg tatgaaattc aatcgctcgt tagaaattcc ggaaggcgta
    48601 ggcagcggta ttttaagcaa cgatgacatc atcggtgtga tgaaaaaatt aatcgaaatc
    48661 cgtaacggtc gtggtgaagt ggatgatatc gaccacttag gtaaccgtcg tatccgcagc
    48721 gttggtgaaa tggcagaaaa ccaattccgc attggtttgg tgcgtgtaga acgtgcggtg
    48781 cgtgagcgtt tatctttagg cgatttagat ggtgtaacac cacaagattt aatcaacgct
    48841 aaaccgattt cagcagcagt gaaagagttc tttggttcat cacaactttc gcaatttatg
    48901 gaccaaaaca acccgctttc agaggtaaca cataaacgcc gtatctcggc attaggcccg
    48961 ggtggtttaa cccgtgagcg tgccggcttt gaagtgcgtg acgtacaccc gactcactat
    49021 ggtcgtttat gtccaatcga aactcctgaa ggcccgaata tcggtttgat caactcacta
    49081 tcggtgtatg ctcgtactaa taactacggt ttcttagaga ctccattccg taaagtggtg
    49141 aatggtcaag taaccgaaga gatcgagtat ttatctgcga ttgaagaaag caacaatatc
    49201 attgcacagg cgaactcaaa cttagatgag aacttccgtt ttaccgatac ttatgtaact
    49261 tgccgtcagc aaggcgaatc gggcttattc aaaccggaag aagttcaata tatggacatc
    49321 tcaacccaac aagtggtttc tgtggctgcg gcgttaattc cgttccttga gcacgacgat
    49381 gcgaaccgtg cgttaatggg tgcgaacatg caacgtcagg cagttccaac cttgcgtgcg
    49441 gacaaaccat tagtcggtac cggtatggaa aaaccaattg ctcttgactc aggtgtagcg
    49501 attgtcgcaa aacgtggtgg tacggtgcag tatgttgatg cgtcacgcat tgtagtgaaa
    49561 gtaaatgaag atgaaaccat tccgggcgaa gcgggtatcg atatttataa cttaatcaaa
    49621 tacacccgtt caaaccaaaa cacctgtatc aaccaagtac cttgtgtgaa cttgggtgag
    49681 ccggttgctc gtggtgaaat cttagcggac ggtccttcaa cagacttagg tgaattggca
    49741 ttaggtcaaa atatccgtgt ggcatttatg ccttggaacg gttataactt cgaagactca
    49801 atgttagttt ctgagcgtgt ggtacaagaa gatcgcttta ccactatcca cattcaagaa
    49861 ttatcttgtg tggcgcgtga taccaaatta ggtgcagaag agatcaccgc tgatattcca
    49921 aacgtgggtg aatctgcatt aagcaaatta gatgaatcag gtatcgttta catcggtgca
    49981 gaagtgaaag gtggcgacat cttagtaggt aaagtaactc ctaaaggtga aacccaatta
    50041 acaccggaag aaaaactatt gcgtgcgatc ttcggtgaaa aagcctctga tgtgaaagat
    50101 tcatcattgc gtgttccaaa cggtacttca ggtacggtta tcgatgttca agtatttacc
    50161 cgtgatggcg tagagaaaga taaacgtgcg aaagaaatcg aagagatgca actgtgtgaa
    50221 gcgaagaaag acttagcgga agagttggaa atcttagaag ccggtttatt tactcgtgtt
    50281 cgcaacctat taattgaagg tggcgtaaaa gaatctgcat tagatggcgt tgctcgtgaa
    50341 aaatggttag aacaaaccct tgaagacgaa gcgaagcaaa atcagttaga acagttagcg
    50401 gaacagcacg aagagttacg caaagaattt gaacgtaaac ttgagatcaa acgcaacaaa
    50461 attatccaag gcgacgattt agcaccgggt gtgttaaaag tagtgaaagt ttaccttgcg
    50521 gtgaaacgcc aaatccagcc gggtgataag atggcgggtc gtcacggtaa caaaggggtt
    50581 atctcgaaaa tcaaccctgt ggaagatatg ccatacgatg aaaacggtca gccggttgaa
    50641 atcgtattga acccgctggg cgtaccgtct cgtatgaaca tcggtcagat cttagaaacc
    50701 cacttaggct tagcggctcg tggtatcggt gatcagattg ataagatgat caaacagcaa
    50761 caagaagtgg ctaaattacg tgaatatatg caaaaagcct acgatttagg tcacggctca
    50821 caagtcgttg atttaagcac tttctctgat gaagaagtga tgcgtttagc gcaaaactta
    50881 cgcaaaggct taccgcttgc tactccggta ttcgatggtg cacacgaaag cgaaatcaaa
    50941 ggcttattag agttaggcgg cttaccgact tcaggtcaga tcacacttta tgatggtcgt
    51001 accggtgaga aatttgagcg tccggtaacc gttggttata tgtatatgct caaattgaac
    51061 cacttggttg atgataaaat gcacgctcgt tcaaccggtt cttatagcct tgttactcaa
    51121 caaccattgg gtggtaaagc tcaattcggt ggtcagcgtt tcggtgagat ggaggtttgg
    51181 gcattagaag catacggtgc ggcttatact cttcaagaaa tgcttacggt gaaatcggac
    51241 gatgtgaacg gtcgtacgaa gatgtataaa aacattgtag atggtacaca ttatatggag
    51301 ccgggtatcc ctgaatcctt taacgtaatt accaaagaga tccgtgcatt agcaattgat
    51361 atggagctgg acgaagcgta atggtttcac tctccccctt gatgggggag agacaggcaa
    51421 aggatttgtt cagaatcctt tggcagagag agggtgatgc aagcggtgta aatttgctaa
    51481 atttttgcaa aaatcgaccg cttaccctct ccctgatttt gcttctgaac gaggcaaaat
    51541 ctatccctct cccacaaggg gagagggttt aaaatcgcaa ccaaattcgt agagtggata
    51601 ttctttccac caaacaaaac aacctcaaac taggggcaaa aaagtgaaag acttagtgaa
    51661 gtttttaaaa gcacaaacaa aatcgaacga agattttgat gtgattaaaa ttggtttagc
    51721 atcaccggac aagatccgtt cttggtcttt tggtgaagtg aaaaaaccgg aaaccattaa
    51781 ctatcgtacc ttcaaaccgg aacgtgacgg tttattctgt gcacgtattt tcggaccggt
    51841 gaaagattac gagtgcttgt gtgggaaata taaacgttta aaacaccgtg gtgtaatttg
    51901 tgaaaaatgt ggtgttgaag taacccaaac caaagtacgt cgtgagcgta tgggacacat
    51961 tgaattagct tgcccagtcg cacacatctg gttcttaaaa tcactgccgt cccgtatcgg
    52021 tttaatctta gatatgccgt tgcgtgatat tgaacgtgtg ctttattttg aatcttatgt
    52081 agttactgag ccgggaatga ccgatttaga gaaaaatcag ttattaaccg aagaacaatt
    52141 tatggacgct gaagagcgtt ggggcgatga atttgaagcg aaaatgggtg cagagggtat
    52201 ccaagcatta cttcgtgatt tagatttaga acaccaatgc gaaaccttgc gtgaagagtt
    52261 acaagaaacc aattcagaaa ccaagcgtaa aaaaatcact aagcgtttaa aaaccttaga
    52321 agcattctta cagtccggta ataaaccgga atggatggta atgacggtat taccggtact
    52381 tccaccggat ttacgcccgt tagtaccgct tgatggtgga cgttttgcga cttctgactt
    52441 aaacgattta taccgccgtg taattaaccg taacaaccgt ttaaaacgct tgttagattt
    52501 agttgcaccg gacatcatcg tgcgtaacga aaaacgtatg ttacaagagt cggttgatgc
    52561 gttattagac aacggtcgcc gtggtcgtgc gattaccggt tcgaacaaac gtccgttaaa
    52621 atctttggca gatatgatca aaggtaaaca aggtcgtttc cgtcaaaact tattaggtaa
    52681 acgtgtagat tactcaggtc gttcggtaat taccgtaggc ccatacttac gcttacacca
    52741 atgtggttta ccgaagaaaa tggcgttgga attattccgt ccgtttatct actcaaaatt
    52801 agaaaaccgt ggcattgctt caactattaa agccgcgaag aaaatggtag agcgtgaaga
    52861 tcctatcgta tgggatattc ttgccgaagt gattcgtgaa cacccgattc tacttaaccg
    52921 tgcaccaaca cttcaccgtt taggtatcca agcgtttgag ccgttattga ttgaaggtaa
    52981 agcgatccag ttacacccac tcgtgtgtgc ggcgttcaat gcggacttcg acggtgacca
    53041 aatggcggtt cacgttccat taacccttga agcacagtta gaagctcgtg cgttaatgat
    53101 gtcaaccaac aacgtacttt caccggcaag tggtgatccg attatcgtac cgtctcagga
    53161 cgttgtactc ggtctttact atatgacccg tgagaaagta aacggtaaag gtgagggaat
    53221 gtacttcctt gaccctcgtg aagcggaaaa agcataccgt accggtcagg ctgaattaca
    53281 ttctcgtgtt aaagtgcgtg ttactgaata tgagaaaaac gaagcaggcg aattagaagc
    53341 aaaaacttcg ttagtagata ccaccattgg tcgttcaatc ttatggatga ttgcaccaaa
    53401 aggtatgccg tttagcttgt tcaaccaaac cttaggcaaa aaagcgatct cgaaattaat
    53461 taacgagagc tatcgccgtt taggtttaaa agaatctgtg gttttagctg accaaattat
    53521 gtacaccggc tttgcttatg ctgctcgttc tggtgcctca gtaggtatcg acgatatggt
    53581 aattccggag caaaaatacg caatcattga agcggctgaa gcggaagtcg ctgagatcca
    53641 agagcaattc aacggcggtt tagtaacagc cggtgagcgt tataacaaag tgatcgatat
    53701 ttgggctgct gcaaacgaac gtgttgcgaa agcaatgatg gaaaaccttt caacggaaga
    53761 agtaatcaac cgtgaaggta atccggaaaa acaagcctca tttaacagta tctttatgat
    53821 ggcggactcc ggtgctcgtg gttctgctgc acagattcgt cagttagcgg gtatgcgtgg
    53881 cttgatggct cgtccggacg gttcgattat cgaaacccca attactgcaa acttccgtga
    53941 aggtttgaac gtattacagt actttatttc aacccacggt gcacgtaaag gtcttgccga
    54001 tacggcatta aaaacagcga actccggtta cttaacccgt cgtttagttg atgtggcaca
    54061 agatttagtt atcattgaag atgactgtgg tacgcacgaa ggtatcgtga tgacaccact
    54121 tatcgaaggt ggtgatgtga aagaagccct gcgtgatcgt gtattaggtc gtgtggtggc
    54181 agaagatgtg ttaaaaccgg gtacagatga agtattaatt ccacgcaaca ccttattaga
    54241 tgaaaaatgg tgtgatgtgg tggatcgtga atctgtggat gtgatcaaag tacgttcggt
    54301 agtaacttgt gatacagact tcggtgtgtg tgctaaatgt tacggacgtg acttggctcg
    54361 tggtcactta atcaaccaag gtgaagcggt cggtgttatt gcggcacaat ctatcggtga
    54421 gccgggtaca cagttaacga tgcgtacgtt ccatatcggt ggtgcggctt ctgcggcagc
    54481 aaaagaatct agcatccaag tgaaaaacgc aggtacgatt aaattaacca acgctaaatt
    54541 tgtaaccaac aaagaaggca aaatcgtatt aacttctcgt aatactgaat taactgtcat
    54601 cgacaccttc ggtcgtacca aagaaaacta taaagtgcct tatggtgcgg tgctttctaa
    54661 acacgacggt gcagaagtgg ctgtgggtga agtggttgcg aactgggatc cgcacacaat
    54721 gccggtaatc tcagaggtat caggtcgtat ccaatttagc gacatcgtag atggcttaac
    54781 cgttactcgc caaaccgatg aattaaccgg tttatcatct atcgtggtgc aagatgtggg
    54841 tgaacgtgca accgcaggta aagatttacg tccggcatta cgcttagttg atagcaacgg
    54901 caaagacatc ttaattccgg gtacagacgt tgcagcacaa tacttcttac cgggtaaagc
    54961 aatcgtaacc ttagacgatg gtgcagaaat tgaagtcggt gaagccttag cccgtattcc
    55021 acaagaatct gtggggacga aagatattac cggtggtctt ccacgcgtag cagacttatt
    55081 cgaagcccgt aaaccgaaag agccggcaat cttggcggaa atttcaggta ttgtgtcgtt
    55141 cggtaaagaa accaaaggta aacgccgctt ggtaattact ccggcggaag gcgaagcgtt
    55201 tgaagagatg attccaaaat ggcgtcagct caacgtattt gaaggcgaga tggtacaacg
    55261 tggtgatgtg atatcagacg gtgcagaaac tccgcacgac atcttacgct tacgtggcgt
    55321 tcacgccgtt accgactaca tcgtaaacga agtgcaagaa gtttaccgct tacaaggggt
    55381 aaaaattaac gacaagcaca tcgaagtgat tgttcgccaa atgttgcgta aagctgtgat
    55441 caccaacgct tacgactctg aattcttaga gggcgaacaa gtggaagtgg ctcgtgtgaa
    55501 aattgccaac cgcaaacgtg ctgaagaagg caaaccgttg gtagaatttg aacgtgaatt
    55561 gcttggtatt accaaagcat ctcttgcaac agaaagcttt atttctgcag cctcgttcca
    55621 agaaaccact cgagtgctta ccgaagccgc agtggcaggt aaacaagatg aattgcgtgg
    55681 cttaaaagag aacgtaatcg ttggtcgctt aatcccggca ggtaccggtt ttgcttacca
    55741 ccaagcacga gctaaaaaac gtgcccaact ggaagcagga attgcagttg tccctgaatt
    55801 tgaagcaccg gcagctaaag ccaattcatt cgttaccgat gcagacatcg aagctgaatt
    55861 tgaatttgtt gcagacgatg caacccaaag tttagcagcg ttgttaaatg cgggtgagga
    55921 agagtaatag ctcgtagggt atgataaatt aaaaaatctc ctagaatata atttctagga
    55981 ggttttctac ttgaatataa gggaaaaata tatagaatgt aagggcttat tcaatttgtg
    56041 gataaggtaa tcaacagtat gtcaatagtt gataacattt cataaagcgt ttcagctact
    56101 gaacataaac taataaagaa tttgataatg attattgtca tattagattc cttgtgagtt
    56161 ataatagttg attgattatt ataatatata tttagcagat agcggtttat tcgatgggta
    56221 tcctaaggta cctaggctat ataaagactg ttaactattc aaatagttag ggacaaccgc
    56281 aatccttagt ctttagtttt gctttcatta aattttctat tatggatata tcttggtata
    56341 agcctcgtaa ttacttacat ttcgattcac ccataactct tcaacaagca attaaattag
    56401 tcgctaatcc aaaacatagt ttctacccat tcttatctta tacactatct gtggctaaaa
    56461 taaaaaagga taaaaatgaa ggtaaattag tacgtttaat aaaaaataga ggaatatctt
    56521 attcatctca taaagatagc catattttta gttattatgc atatttatta aatgaaaagt
    56581 atgaaaatct tttgaaaaat aagtactata ttttagatga ttctatatta gcatttagaa
    56641 aactaaataa aagcaatatt gattttgcca gaatcgcttt tgatagtatt cgaagcgctc
    56701 aaaattgcac ggttttaggg ctagatatat ctaagttctt tgaccatata gaccataaaa
    56761 ttctgaaaga tatgtggtgt aaagttttgg gtgtacctag tttacctgaa gaccatcatt
    56821 ctgtatttaa ggctattact aaattcgtaa aagtagatag agatactgta tttgaattct
    56881 ttaatatctc ccttcataat cctaggaaaa atgggaagaa tagaatttgt agccctcggg
    56941 aatttcgaga atataggaaa ttaaagaaac ctgacttatt taatgataat ccagcttttt
    57001 ttattaataa aggccagaaa gggattcctc aaggttctcc aataagtgca ttgttatcca
    57061 atatctatat gttagagttt gatgtgttag ttaaagaaaa gattatggag tgcaaaggga
    57121 gttattttcg ttattgtgat gacattctat gtatcattcc taatgaatac gaagaattta
    57181 ttctcgatta catcactggg gagataaaaa gtaaattaaa actagagata aataaggata
    57241 aaactgaggt tgttaaattc caatattgta atagaacaaa aaaaataatt aatgaaaaga
    57301 agttacaata tcttggtttt atattgcata ataactctat tagtattaga tcatcggcat
    57361 ttacacgata ttcaaataaa atgaaacgag gggttagtct agctaagcaa actcagggta
    57421 aatataatag gataagaata agacgagggg tagctatcaa aggcatttat aaaagaaagc
    57481 tattttctaa gtattctcat ttcggaaaat ctaattttat ctcttatgga aagagagctt
    57541 attcatctat ggattccaaa gttataaaga accaacttaa acctttatgg tatagattaa
    57601 agaaagaaat atatttgtaa tattagaaag tgtaagactt gtccttgcct gcaagtgttg
    57661 gaagcccata tattgcttta tcaatcctat tccctcgata gcgcccgtct ccagacgggt
    57721 gcttgtttaa gcataatgaa agtttgtata agcactcgtc agagacgaac accatcataa
    57781 ttgctatcgt aagagaaatt tgcactacaa gcggtcaaat tccctcaaaa acttgcaaat
    57841 tattgagtaa acccaaccgc ttgtcattct tctaaagctg agcaaatgcc ttatccgcag
    57901 cctctagcgt acgagcaata tcttcatcac tgtgagctaa agacataaac cctgcctcga
    57961 atgccgatgg ggctaaatac acgccttgtt ccagcattaa gtggaagaaa cggttgaatt
    58021 tggcggcatc acatttcatt acttcttgga agttgtttac tgctttttgt tcggtgaaga
    58081 ataagccgaa cattccgccg acatattgta ccgttaatgg cacattgtgc ttatctgcaa
    58141 gagctttaaa gccttcagcg agggttttgg ttttttccgc taaaatggtt tcatttcctg
    58201 cttttgataa ttcagttaag catgccaaac ctgcagccat tgcaatcggg ttgcctgaga
    58261 gcgtgccggc ttggtaaacc gggccagttg gagcgatgta ttccataatc tcttttttac
    58321 caccaaatgc ccccaccggc attccgccgc cgataatttt gccgagtgtg gttaaatccg
    58381 gtgttacccc atagtaagat tgagccccac cgagtgcgac acggaagccg gtcattactt
    58441 catcaataat aaataccgca ccatattgtg tgcaaagatc acgtaagcct tgtaaaaagc
    58501 cttcttgtgg cggaatgcag ttcatattgc cggcaacagg ctcaacaatt aaacaagcga
    58561 tctcatttgg gtattgctca aatgctgctt ttactgaatc taaatcatta taggtgcaag
    58621 taagcgtatg cttggcgaaa tcagcgggta cgcccgggcc gcttggctga ccgagggtta
    58681 aagcacctga accggctttt actaacagag aatcggaatg cccgtggtag cagccttcaa
    58741 atttgatgat tttatctcgg ttggtatagc cacgagccaa acgaatggca gacattgttg
    58801 cctctgttcc gctgctcacc attctcacca tttcaataga aggtactaat ttgcacacta
    58861 attcggcaag tgtgatttct gctgccgttg gggcaccgaa acttaagcct tttggcacgg
    58921 cttttagcac cgcatcaata atcgccgggt ggttatgccc gagtaccatt ggcccccaag
    58981 agccgacata gtcgatatat tgtttgccgt cgctgtcggt aatataagca ccttcggctt
    59041 tttcaataaa aaccggtgtg ccgcccacgc ctttaaatgc acgcacaggg gagttcacac
    59101 cgcccggaat cactttttgg gcttgttcaa acagttgttc tgaaatagac atatcaattc
    59161 ctttcttttg atgtaaaaat ttgcctgtta ttgtactgaa aagcccttgt tttcgctaaa
    59221 aaatttacga ttttgttgcc ttatgctatt ctgcattcct attttatgac tcattttttg
    59281 aggcaatccc tgtatgtggc ttactttttt aaccgtattt attgtttctt ttttagccct
    59341 gattgtgatg cgtccggtgg cggaatttgt tggtttggta gataagccca acttccgtaa
    59401 acgccatcag gggttgattc cgttaatcgg tggtattgca ttatttatcg gcaatctcac
    59461 tttctatttt atgcagtggc aagatatgcg gctgccggaa ctgtatctga cagcggtaac
    59521 tattttgctg attatcggcg tgttagatga ccgatttgac atcagcccat tactgcgtgc
    59581 cgggattcaa gcagcactag ctggggcgat gatttacagt ggcttgtcgc tcagcagttt
    59641 agggcaaatt attgccccct ttagtataga attaggttgg ctcggcattg tctttaccgt
    59701 gtttatcacc attggcgtta ttaatgcgtt taatatggta gatgggattg atggcttatt
    59761 agcagggctt tccagcgtga gttttgccgg tcttggcact ttaatgttca ttaatggcga
    59821 accaagtttg ggctattggt gctttgccct tatctttgtg ttgttgccgt atgcaatgtt
    59881 caatttgagc ctgtttgggg cgaaatggaa agtgtttatg ggcgactcag gcagtatgct
    59941 gatcggcttt accattattt ggattctgct gttaagcacc caagggcaag gctcgccaat
    60001 tagcccgatt accggtttgt ggctgattgc cgtgccgtta attgatatgg ttgcggtgat
    60061 tatccgccgt ttgaaaaaag gtaaaagtcc gttcaaacca gaccgcttgc acctacacca
    60121 tttaatgatg cgagcagggc ttacctctcg ccaagcatta ttggtgatta ccctctgggc
    60181 gggattttgt tccaccattg gggtattagg cgaggtgtat tattggaatc agtgggtgat
    60241 gatgctaatg tttatcgggc tgtttttcct atacgcctat tccattaccc acgcttggcg
    60301 tgtaacccgt tttgttcgcc gtctaaaacg ccgtgcaaaa cgtagcaaac aactttaaag
    60361 gatatgttgt gaaaacctta ttttatcgac ttttagtgct aattttattt gctgctatcg
    60421 gggcaggtgc cggttatggg ctgagttata ctcaacctga aaaatggaaa gtgaccgctc
    60481 aatttgagca accgactatt cccgatttag gtaattatta ctcgttactt tccacctacc
    60541 attttttgaa tagtaatagc caagaagcga atagccaaac ggcacagcaa caggcaagtg
    60601 agcaggttta tgcggaattt aaacgcaacc tcacgtccgc cgacttgctt caagcctatt
    60661 tgctacaaac agaagcggtg aaattaaaag ctcaggttgc aaagaaatca ccggcggtgg
    60721 tggcggctga attagttgaa caattccgtt ttgaaaaaga tatggcaact tcggcagata
    60781 atgtcagctt aattgcagaa aatgcggaag aggctcaaaa attattagcc gattttattc
    60841 cgttggtaaa cacccaagct cgagataaat taaacgccga tttaattgcg aaatggaaag
    60901 tcttgttcca gcaaattaaa actgcttcgg agttgaatat taacggtaag caaggctcac
    60961 aagattgggc agctaaactg caaatgatgc gttctgttca gccgttagac aaccaactta
    61021 cagcttatcg cttggttaaa tcgcctagcg ttccacttaa atcagaatcg cctgatccgc
    61081 ctattttggc tgatgattgg ggcattaagt ggcttactgc tgggctttat gctcagtttt
    61141 gcggtgaaag cgaaagctca acctgcggaa ctttaatcta aaatggcaca caatttagtg
    61201 tgccatttct ctttgtaact gatgatgagt aaaactgaat ttaacactat ttgtattctg
    61261 gggttaggct atatcggctt gccgacctca gtcgtttttg ccaatgccgg caagcaagta
    61321 attggggtgg atattaatcc gcaagtggtg gcaagcctta atcaaggcaa cattcatatt
    61381 atcgagccgg atttacaaac tgccttcaca caagcggtca aaaaaggtaa tttttttgca
    61441 actgccaaac cgcaaccggc cgatgctttt atcatcgccg tgccaacccc actttgcggg
    61501 aagcagcccg atctcactta tattcaaaat gccgccgcta tgattgcccc ttgtttgcgt
    61561 aaagggaatt tggtggtgct ggagtccact tcgcctgtcg gtacaacaga aaatttagcc
    61621 gaatggctaa gccaagctcg ccctgattta gcctttccag ccgatgtgtc tattgcctac
    61681 tgccctgaac gtgttttgcc gggtaatata atgacggaat tggttgaaaa tgaccgcatt
    61741 atcggcggat taagcgaaca atgcagtcaa caagcggtcg atttatacca aatttttgca
    61801 aaaggacgtt gtgtcaccac cacagctcgc acagcggaga tgtgcaagtt ggtggaaaac
    61861 agctttcgtg atgtgaacat tgcctttgcc aatgaaattt caatgatttg tgatgcgttg
    61921 aagatcaatg tgtgggagct aattgaattg gcaaatcttc acccgagagt aaatattctc
    61981 cagcccggag caggggtggg cggacattgt ttggcggttg acccgtggtt tatcgtttcc
    62041 acatcgcccc aacaggctcg ccttattcgc acggcacgag aagtgaatga cagcaaaccg
    62101 caatgggtaa tcgagcaagt gaaaacatcg gtcgcagact gtgcgatgga gcaagattgt
    62161 aagccaagtg agctaaccat cgcttgttta gggctagctt tcaagccgaa tattgatgat
    62221 ttgcgggaaa gcccggcatt agcgattacc cggcaacttg ccgcttggca tcagggtagc
    62281 ctgttggcgg ttgagccaaa tattcaacag ctggctggcg aaaaaatcac cttagtcgat
    62341 ctcaacaccg cactttcgca ggcagacatt ttagtcttgt tagtggatca cacttcattt
    62401 aagcaaattt caagggaaca gatcaggcaa cggcatatta ttgattgtcg aggagtgtgg
    62461 gtaagatgaa gccggatcaa tggctgtccg attttttcgg tagagaaatt gtgcaagtaa
    62521 aagtagcggc tgatgatttt aggcaaatcg ctaggttgca aaaacaaggt tttgtacttg
    62581 tggagggaga attggaattt gagctggcac ttgcaacatt ttctgaaaat atgaccgctt
    62641 gtcgcccggc ggatatggca gatttgcccg acttgaactt attgtttggt tcggcatttc
    62701 ccgatagccg gtttcgttcg ccctattttt caagggaaga gaatcaacgc ttttaccgga
    62761 cttggattga aaatgcggta aaaggcaagt ttgatgatat tggcttggtt caatgcaatg
    62821 ctgacgggca aattcaaggg gcggtaacgg ttcgcttact tgcccaacag caagcgaaaa
    62881 tcggattatt ggcggtaaag cctgattgcc gacaacaagg tattggctcg gaattgttaa
    62941 atgctgcgat agcgtgggcg aaacagcaaa atgcgaaaaa actgatgatt accactcagt
    63001 tgagtaacca gaaagcgatt cgtttttatc aagcgtttgg tgcaagcatt aaggcggtgt
    63061 attattggtt ttaccctgct ttggaaaaat acgatgatga acaatcctga ttttattccg
    63121 tttaatcaac ccccggttgt gggggcggag caagcctata tagaacaggc gatggcaaca
    63181 gggcagcttt cgggcggtgg taaattcacc cgcttatgtg agcagtggct tgaaaacctg
    63241 gcccgtagca aagtgttgct taccccttct tgcacggctg cgttagagat ggcggcaata
    63301 ctgctggata ttcagccgga cgatgaagtg attatgccga gctatacttt tgtctctact
    63361 gcaaatgcgt ttgcgttgcg tggggcaaag attgtgtttg tggatattcg tccggataca
    63421 atgaatattg atgaacgcaa aattgagcaa gccattaccc caaaaaccaa agtgattgtg
    63481 ccggtgcatt atgccggcat tgcttgtgat atggattcaa taatggcaac agccgaaaaa
    63541 tacgggcttt atgtggtaga agatgcggca caggcagtca attcgtttta taaaggtagg
    63601 gcgttaggtt ccatcgggca tattggttgc tatagttttc acgaaaccaa aaactatacc
    63661 gctggcggtg aaggtggggc gatcgtgatt aatgaggcga atttgctgga aaaagcggaa
    63721 atcatcagag agaaaggcac taaccgtagc caattttttc gtggtgaggt agataaatat
    63781 cgctggcagg atattggttc aagtttttta atgtcggaaa ttcaagcggc ttatttgtat
    63841 gctcagctta tagaggcaga taaaatcaac caacagcgtt taaccctttg gcacaattac
    63901 tatcaagcct ttttgccact tgcccaaaca gctaagtgcg agcttcctcg cattggcgaa
    63961 tattgtcgcc ataacgggca tttgttctac ttaaagctaa gtgacttgaa gcagagaact
    64021 gcttttattg attttatgaa ggcacagaat atcttaaccg tgtttcatta tgtgccgttg
    64081 cacagcagtc cggcagggca gaaattaggt cgattagcag gtgatgatcg ctttactacc
    64141 ttagaaagcg aacggttggt aagattgcca ctgttttata atttgagtaa gcaggagcaa
    64201 gagcgtgtta ttcaagcagt gcttgctttc ttcaatcaat aaaaaacggc tgagtgtttc
    64261 agccgttttt ttgctatatg ttgttactat gaatagtaaa gttcaaattc taccgggtgc
    64321 ggagtcatat ttacacgttc cacctctttg cgacgaatac cgatataagc atcaataaag
    64381 tctttcgtga acacgccacc ttgtgttaag aattcaaaat cattgcttaa tgcgtcaaga
    64441 gcggattcta aagacgaggc aaccgttgga atatctttta actcttccgg cggcaaatcg
    64501 tataagtttt tatccattgc atcgcccggg tggattttgt tgatcacacc gtctaagcca
    64561 gccattaaca gggcggcaaa gcaaaggtat gggtttgcca ttggatccgg gaagcgtgct
    64621 tctacacggg tcgctttcgg actggtcaca gccggaatac ggatagaggc agagcggtta
    64681 cttgcagaat atgccaataa caccggtgct tcaaaacccg gcactaaacg tttataagag
    64741 ttcgtggttg ggttagtaaa ggcatttaat gcttttgcgt gtttaatgat accgccaata
    64801 tagtagaggg cagtttcaga taagccggcg tatttatcgc ccataaacac atttttgccg
    64861 tctttgctta atgacatatt acagtgcatt cccgagccgt tgtcgccggc gaacggtttt
    64921 ggcataaagc aagcggtttt tccgtgttct acggcaacat tttgcacgat gtatttatag
    64981 atttgggttt catcggcttt taaggtcagg ctgttgaatc gggttgcgat ctcattttgt
    65041 ccggctgtcg caacttcgtg gtggtgagct tcaatcacta aacccatctc ttccatcaat
    65101 aaacacattt cagagcgaat atcgtgggcg gtatcaatcg gtgcaaccgc acagtagccg
    65161 ccttttttca acggacgata ggctttgttg ccttcttcaa atttacggtt ggtattccaa
    65221 gccgcttcta catcgtcaat tcggtatgac acattgttca tttcggtgct aaaacgcaca
    65281 tcatcgaata agaaaaattc aggctcaggt ccaaacatta cgctatcggc gataccggtg
    65341 gatttcaagt agttttcggc acgggttgca atagagcgag ggtcacggtc ataagactgc
    65401 attgtgtttg ggtcgtaaat ggaacaacga atagaaaggg taggaatacg ggcgaaaggg
    65461 tcaactacag cggtttcagc aatcggcatt aacaacatat ctgctttgtt aattgccttc
    65521 caaccttcta cggacgaacc gtcgaacatt ttgccttcct cgaataagtc ttccaaatcg
    65581 ttggcaacta agctgacagg gagcgaaacc ccgtgttctt tacctttaat atcagtaaat
    65641 tttaataaga caaatttaat atcattgtct tgaatcagtt tagatacatt agcgattgcg
    65701 tttgacatag taaaataagt cctcgttggg ataaatgaat aataaaaaag ctcggttatc
    65761 atagcatttt ttaaccaagt gtgtgatgtt ttttagtgtt tcaaagctaa aagaagttgc
    65821 agtaatgttg caaatttttg ctaaaatctt accgcttgca tattgtttgt atggtgggga
    65881 gagaaaagcg tgtaatgttg ccgatttatc atattctagg ctccgagatt gagcatcata
    65941 accaacaggt tttaaccttt tttcagcaag agcttttgcc gttattgcca gactccgaac
    66001 atttttttta tgtggtaggc aagccttcac tgcaaactgc ttttactcat ttgcggatta
    66061 agcagttctc ttcacaaaaa gcgattgcga acgccttaat tgcaaaaaaa cggcaaaatc
    66121 cgaccgcttg ttttctgtta cacgggcagt ttaacccttg gatttggctg gcgatagcct
    66181 taggcagatt gccgaccacc aacctgattt ggcatatttg gggggcggat ttatatgaaa
    66241 tggcaacttc gtggaaattt aagctgtttt acccgtttcg gcgtgttgcc caacaaaaaa
    66301 tcttacaggt ttgggcaaca agaggcgatc tggatcattt gtggcgaaat atgcgtgcaa
    66361 gatcagaaca ggatcattta atctatttcc ccacgaaatt aggtgatcaa ataaaaatta
    66421 gccccacaca aaatgatcgc ttgacgatat tgctcggcaa ttcaggcgat ccagcgaatg
    66481 agcttattca agccttaagc gagattcatc aacgactagg gaacaatgtg cggattatca
    66541 taccaatggg ctacccggaa aataatgaaa gctatattcg gcaggtggaa gcctatagca
    66601 agcggttatt tttggcggaa aatttgcaaa ttttgcggaa gaagctgagc tttgaagagt
    66661 atgttacgtt attgagccat tgtgatctcg gctatttcaa ttttaaacgc cagcaaggca
    66721 ttggcacgat aagccttttg attagccata atgtaccttg tgtgttacat tcagccaatc
    66781 cgtttgggtt ggatctacag gcagaaaaag tgccttattt gttggcggaa aatctgactg
    66841 cttcacagat tcaaacaacc aaacaacaac taaagagcat agataaaaac cgcattgcgt
    66901 tttttgctcc gggctacact aaactatggc aacagcgatt aatcgaatta atgcaaaatg
    66961 aataatttaa taccacttgg cggattttat cttgtttctg tcgggctatt tttcagctta
    67021 ctttatcaac tttatcagaa atcggggatt tctttccatt tactgtttag tttgctctat
    67081 tttgttactt tttacgccgg ttttccgttt tcgttaataa tggcatttgg ctttgattac
    67141 ccgctgccgg agctaaaaac acaatggcaa gtttttacgg cagcaggtgt tggctatttg
    67201 atttattacg ttgtctattc acgcttgtgg agtgctgtgt cgcaaccaac tgctcttgtt
    67261 gcaaattttc aggcaaaaac gaccgcttgt atccttgccg ctgtggcagt tgttgccctg
    67321 ctatatttcc tttatttaaa cggttttctg cttttcaaac tcgaaaaata tagccaactg
    67381 tttgccaaag atagggtcat actttcggca ttaaaacgat ttttctactt tttattgccg
    67441 gcgttgttaa ttttttgctt cctatataag aataaaacag cgtggtgggc gttactgatt
    67501 gttggtgggg cattcggcat attaagctat gtggcagtgg gcggcacacg ggcaaattta
    67561 gcactagcgg ttggcttttt cttattgatt ggattgcagc aaggctatct gtcgttatca
    67621 aaggtgattg ccgtaagcct aatcggcgtg gtggcgatgt tcttcctcgc tctagcccgt
    67681 tacaaactta ccctttccgg tagcgagatg atttacacct tcttatattt aagccgagat
    67741 actttttcgc cttgggaaaa tttggctcgt gtattttcta atgaggtgga atatcaaggt
    67801 ttaatgccga ttgtgcggga tttctatgta tatatcccac aatctttatg gcaagaacgc
    67861 cctgagatcg tctggaatac ggcgaattat tttaccaaag tgattttagg caaccaatca
    67921 gggttagcta tttcgcctac cttactcggc tctttctata ttatgggcgg atttagcgtt
    67981 atggcgttgg gaatggggat agtagctgcc atgattaaag ccttagataa cctattcgct
    68041 tacgccaaag cgtgcaattc cgccttgttg caagcctact gcttcggcaa tttattcaat
    68101 ttgatcgtat tagtgcgaga gggaatcgat gcatttgttt cccgtttttg ctttttcacg
    68161 ctgatattta tggcttgcta tttgctggcg aaaatccttt caatggggaa aaataataat
    68221 gttcgataaa gtgatcattc ggggcattgc gttacaagcg gtcaaaaatc gggaaatttt
    68281 tgcaaattgg ctgatgaatg aaaagcggat tcagttcgga agattaatgg caattaatgc
    68341 tgagaaagtg attctgtcgg aaaaaaatcc gttgttacgc cgagtgatag atgaagccga
    68401 gttcaactat gcagacggca tcagtgttgt tcaatcgatt cgaaaaaaat atccaaacta
    68461 taaaatagag cgaattgccg gagccgattt atgggaaacc ttaatgcaaa gagccggagc
    68521 gttaaatgtg ccggtgtttt tggtcggcag ctcggcggat accctaacga aggtggaagc
    68581 aaaattggca caatggaagg tgaatattgt cggtaggcag aatggctatt ttaaggcgga
    68641 gcaagaacag gacgttatag agcaaattaa agcaagtggc gcgaagttta ttagtgtggc
    68701 aatgggaact ccaaaacagg aactttttat ccaacagtta caacaacaat atccacaagc
    68761 cttatatatg ggtgttggcg ggacttacga tgtgtttgcc ggtaaagtga aacgagcacc
    68821 taaaatttgg caaaatttgg ggctggaatg gctttatcga ctattgaaac agccaacccg
    68881 ctggcaaaga caattaaatt tactaaaata tgcctattac tatctgacaa atcagctcta
    68941 aaaaacgcat tttttgatat ttattaaacg ttttgaataa aataaaacca aacaatacat
    69001 ttttttacat tttttttaaa aagtgctaga caagggaaag tgaaatctat aatatacgcc
    69061 tccgttgcga cgcattaagt tgaattgatg taaatcgaag cgttcgtagc tcagttggat
    69121 agagcgttgg cctccggagc caaaggtcgc aagttcgaat cttgtcgagc gcgccagatg
    69181 tcaatgcggc actatcaaca caatggtggc tatagctcag ttggtagagc cctggattgt
    69241 gattccagtt gtcgtgggtt cgaatcccat tagccacccc attcaatact cttgtgagta
    69301 tcagacggcg agtagcgcag cttggtagcg caactggttt gggaccagtg ggtcgtaggt
    69361 tcaaatccta tctcgcc
//
LOCUS       DANKBV010000002        43466 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.002, whole genome shotgun sequence.
ACCESSION   DANKBV010000002 DANKBV010000000
VERSION     DANKBV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 43466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43466
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.002"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..508)
                     /locus_tag="PWY43_000065"
     CDS             complement(<1..508)
                     /locus_tag="PWY43_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016504515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HDL2134991.1"
                     /translation="MSYQLLPNTTIRPELSRHYRFDEQTLVQNLIEQAHCDEQADNIK
                     KLTTQLVGKVRTARQKASGVDALMHEFSLSSQEGVALMCLAEALLRIPDTATADKLIR
                     DKIAKGDWRSHTGNSPSLFVNAAAWGLVITGKLVSTHSESSLSSALSRLIAKGGEPLI
                     RKGVDVAMR"
     gene            complement(628..2139)
                     /gene="nhaC"
                     /locus_tag="PWY43_000066"
     CDS             complement(628..2139)
                     /gene="nhaC"
                     /locus_tag="PWY43_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2134992.1"
                     /translation="MLILQTFSKNQPLVKGGFMSNRTEITDQNIRIPFRYAILPVIIM
                     VSIMAVAIIKFEASPHVPLLIGAISAAVIAMAHGYRWETIEKGIYRGIRMALPAIVII
                     IMIGLTIGAWLGGGIVATMIYYGLKLISPSLFLVTISIICAIVTLAIGSSWSTMGTIG
                     IAAMGIGFSLGIPAPMVAGAVISGAYFGDKMSPLSDTTNLAAGITETNLFEHIRHMFI
                     TTVPAFLIALSVYFYFGMEFRAENLDLAKIEDIMQAMQEHFLISPWLLLVPLLVVVLV
                     AKKVPALPALAAGIILGWLCHVFVQGGDVSVAVITLHDGYKIESGHEMIDKLFNRGGI
                     ESMMYTISLTIVAMTFGGIAEQAGMLHSVVEKILTLAKSAGSLISATIVSAFITNVTT
                     SEQYISILLPGRMYIKAYRDKGLSSRNLSRALEDGGTVTSVLVPWNTCGVYAFSMLQV
                     SAFEYAPYAIMNWLIPLIAIGLATLNIKIERLPPEELAKEPEEITLPKRGCCP"
     gene            complement(2214..3404)
                     /locus_tag="PWY43_000067"
     CDS             complement(2214..3404)
                     /locus_tag="PWY43_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL2134993.1"
                     /translation="MSTSQQLIQQTEHFGANNYLPLPICISKAKGIWVEDPEGNRYLD
                     MLSAYSAVNQGHCHPKIVQALKDQADKVTLTSRAFYNDQLGIWYEKICKLANKEMALP
                     MNTGAEAVETAIKTARRWGYDVKGIAENQAEIIACVGNFHGRTMAAVSLSSDPDYKRG
                     FGPMLGGIKLVPYGDLEALKAAITPNTAAFLVEPIQGEAGILIPPEGFLEQAYELCKE
                     NNVLFIADEIQAGLGRTGKLFATEWEGFEPDIYILGKALGGGVFPISCVLANRDILGV
                     FNPGSHGSTFGGNPLACAVSIASIDVLLEEKLAERSRELGEYFLAELKKIQNPIIKEV
                     RGRGLFIGLELHQPARPFCEKLKEKGLLCKETHDTVIRFAPPLVIEKFDLDWALEKIR
                     EVLA"
     gene            complement(3656..4307)
                     /locus_tag="PWY43_000068"
                     /pseudo
     CDS             complement(3656..4307)
                     /locus_tag="PWY43_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(4404..4988)
                     /locus_tag="PWY43_000069"
     CDS             complement(4404..4988)
                     /locus_tag="PWY43_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HDL2134994.1"
                     /translation="MLTWLRILIHLGCLLPLVWVAQLLYTGNETILGADPIKELEHFL
                     GYGAIVIFCIMFLLGIALQYLKKNQYQILRRPLGLWAFVWAALHISSYLLLELSLDVK
                     LFFSELAGRPYLILGAAAFFILSIMAITSLPMLKRKLGTRWGTIHQWAYPALVLAMVH
                     YYWSVKSVTFDPIIIGVLVLFIVAHKIQAKFAKK"
     gene            complement(4988..5941)
                     /gene="msrP"
                     /locus_tag="PWY43_000070"
     CDS             complement(4988..5941)
                     /gene="msrP"
                     /locus_tag="PWY43_000070"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDL2134995.1"
                     /translation="MKKLTIQDVTPETIFYQRRKIITALGLGIAASALPKVSMAETQR
                     HLQSLQFKPDPNKGLELTPENKVIGYNNFYEFGVDKSAPAKNAANFKTDPWTLEIGGE
                     VENPFSLNHQQLLNTFPLEERIYRFRCVEAWSMVVPWIGFELARLIEMAKPTSKAKYV
                     VFHTLYDPAQMPGQKNRLLGGGIDYPYVEALRLDEALNSLTLLSVGLYGKTLPPQNGA
                     PIRLVVPWKYGFKSIKSIVKITFAEKRPLTTWESLAPNEYGFYANVNPNVDHPRWSQA
                     SERVIGAGGLLSVKRQPTLMFNGYENEVANLYRGLDLKVNY"
     gene            6050..6616
                     /locus_tag="PWY43_000071"
     CDS             6050..6616
                     /locus_tag="PWY43_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2134996.1"
                     /translation="MKSLSSTQIEEIRKQMVKFATLQLADKDLAEDVVQDALTNAYKH
                     SASFRREAALKTWIFAILKNKILDLLRTRKRHIPVSSLCEEEESPSAFFDRHDHWLEH
                     QDVTEWRGIAQSTYQKEFWAIFDICLNKLPAQQARVFMMREHLEMSSAEICQECEISL
                     SNLNVLLHRARLRLQECLSRNWFREESK"
     gene            6613..6798
                     /locus_tag="PWY43_000072"
     CDS             6613..6798
                     /locus_tag="PWY43_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL2134997.1"
                     /translation="MKCRQATELISQSCDEKLRLQQHMRLQLHLLVCPKCRNFNKNTK
                     KLGKILRAYCNDKDAPF"
     gene            6828..7112
                     /locus_tag="PWY43_000073"
     CDS             6828..7112
                     /locus_tag="PWY43_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2134998.1"
                     /translation="MRYWLISLSILLSGCVVPVTPTGQAGIPMPIIIGVSDPLDSKEV
                     HICTLKPFTQEYRSEHSSRGRAKLNVQKQCRANHSEMFCEEKDIVCKTYD"
     gene            7216..8259
                     /locus_tag="PWY43_000074"
     CDS             7216..8259
                     /locus_tag="PWY43_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2134999.1"
                     /translation="MQPINIAIAGAGFSSRVFHIPFLKQDARFNIVKVYERTSNNAAE
                     WLPNAETVRDFNALLTPEVDLVLITTPNQTHYAMVKESLLAGKHVLVEKPLVATVAEA
                     EELAELAKRQGKVLYVYQNRRWDSHIATAKSLMEQNLLGELVDCEIRFDRYAKEKNVK
                     VWKESGELGTGLVYDLGVHLIDQALYLFGKPEAVYADVRYQHEGALVDDNFDIQLYYA
                     NGFKVVLKASRYAREPSPTFVLHGKLGSYLKQTPDNQEDLLKNGVQPIGKDWNIEEKE
                     NWGILHTEVEGNVVRQPYRNAEGSYQNLFDDLYQAIAHNAEPIIKLEDVIFVLKVIES
                     VFESAKLGQKIYL"
     gene            8499..9605
                     /locus_tag="PWY43_000075"
     CDS             8499..9605
                     /locus_tag="PWY43_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HDL2135000.1"
                     /translation="MNKLIASLVASSCATAFAAPVQQNNFHTTTHTYEFTQSYDLVVP
                     QGSKGTTNLWIPLPSDTDYQTVKSIEFEGSYKNAYITENNAYGAKTLFATWDTDAPKR
                     DLKVKMVIQTQDREPMAQGALANYQMPEKITYSVDVLEYLKPTAHIKTDGIVKEYADK
                     IVGNEQNPLKKAELIHKWIVDNMERDNSVLGCGDGDVEKILTTKVLKGKCTDINSVFV
                     ALARASNIPAREVFGIRLGQAVKMGQYSKTAFGSSKDGVANESGGQHCRAEFYLAGFG
                     WVPVDSADVAKMRLAEKKDVDDADVQAVSKYLFGNWEMNWVGFNHGRDFDLYPQPELT
                     PLNNFGYPYAEVGGDPLNSFEAKEFGYEFISKEL"
     gene            9580..9948
                     /locus_tag="PWY43_000076"
     CDS             9580..9948
                     /locus_tag="PWY43_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135001.1"
                     /translation="MNSSLKSYNHKFITMCVTAIVSAVTSTFCCIGPLVYLVFGISSP
                     WLMELNQLEFLQLPMLLISLAAFGYGFWLLNFSNKIICTKYFSRRTLVILYWVVSVVV
                     MFFLTYPYVLPYILEMWRGE"
     gene            9950..10258
                     /locus_tag="PWY43_000077"
     CDS             9950..10258
                     /locus_tag="PWY43_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL2135002.1"
                     /translation="MKKYIATMLLAFGLVHSSYAQEKTAQIETKQAEKTVVLHIPEMY
                     CQLCVYLVNKELRAVEGVISTKASMKERKATVVVKESVDDQALIQAVGNLKYTAKVIE
                     "
     gene            10495..11049
                     /gene="srlA"
                     /locus_tag="PWY43_000078"
     CDS             10495..11049
                     /gene="srlA"
                     /locus_tag="PWY43_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725059.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL2135003.1"
                     /translation="MIEAITHGAEWFIGLFQKGGETLVGMINGILPLLISLLVVMNAL
                     IKFVGQDRIERLAQRSAGNPISRYLILPVIGTFVFCNPMTLSLGRFLPERYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAISYLLVGMFTNFFRGWI
                     TDFTTAFFEKRMGITLDREVHLSH"
     gene            11080..12081
                     /locus_tag="PWY43_000079"
     CDS             11080..12081
                     /locus_tag="PWY43_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL2135004.1"
                     /translation="MSNKAIYIEKGNGGWGGPLTLPVVEGKKVLYMTGGTRPAIVNKL
                     VELTGWEAVDGFKEGEPLAEEIGIAIIDCGGVLRCGLYPKRRIPTINIHSTGKSGPLA
                     EFIVEDIYVSAVKENNIRLIDADGSVPVATEQAKPTSSTTATAKEYDSSKKITEQSDG
                     LLAKIGMGMGSVAALFFQAGRDTIDTVLKTILPFMAFVSALIGIIIASGLGDVIAHGL
                     TPLANSPIGLVTLALICSFPLLSPFLGPGTVIAQVIGVLVGTQIGLGNIPPHLALPAL
                     FAINAQAACDFIPVGLSMAEAKQDTVRVGVPSVLVSRFLTGAPTVLIAWAVSAFIYQ"
     gene            12126..12524
                     /gene="srlB"
                     /locus_tag="PWY43_000080"
                     /gene_synonym="gutB"
     CDS             12126..12524
                     /gene="srlB"
                     /locus_tag="PWY43_000080"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2135005.1"
                     /translation="MHRLSKRENIYMTIIYQTTFTKIGNFAQESLTDDMLITFKQGAP
                     ADLEDYCFIHNPSELSSPLEVGDIAEFDGVAYPITAVGSVASENLSALGHITFRFDGA
                     NDAEFPGSVHVIGTPPQGLTENSTLIIKRD"
     gene            12572..13360
                     /gene="srlD"
                     /locus_tag="PWY43_000081"
     CDS             12572..13360
                     /gene="srlD"
                     /locus_tag="PWY43_000081"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDL2135006.1"
                     /translation="MNNEKKVAIVVGGGQTLGAFLSEGLADAGYRVVVADSNGENAQK
                     VAADINAKQGEGNAIGVQVDAADEPSVEALAKATDTVFGRADVLVYSAGTAKASPIWD
                     FNLNDFDLSVKVNLTGYFLTAKHFSRLMIRDGIKGRIIQINSKSGKVGSKYNSGYSAA
                     KFGGVGLTQSLALDLADKGITVHSLMLGNLLKSPMFQSLIPQYAAKLGIPESEVEQVY
                     IDKVPLKRGCDYQDVLNVLKFYASEEAAYCTGQSINITGGQVMF"
     gene            13529..13909
                     /gene="gutM"
                     /locus_tag="PWY43_000082"
     CDS             13529..13909
                     /gene="gutM"
                     /locus_tag="PWY43_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDL2135007.1"
                     /translation="MDSITLLIILAMIMWLLQAVLGWLQIKAFNQAFMEISKKGKVTV
                     GRNSGRFNPKSVIVLAFDDEKKVVDSLCMKGFSVFARPQKLASVIGLSLDQIVPEKIF
                     PSDKRSQFALKVAISSGYSCSDQK"
     gene            13972..14619
                     /gene="hxpB"
                     /locus_tag="PWY43_000083"
     CDS             13972..14619
                     /gene="hxpB"
                     /locus_tag="PWY43_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDL2135008.1"
                     /translation="MQINSVIFDMDGVMIDSEPQWAEAQIAVLADLGINITEQDCEQL
                     TRGKRIDELSDTWIKKFQLSVSNQALSEAILAHGCAAIEQHGKALTGLYELLDFLKSN
                     QIKLAVATSSPPVVIKAVFDRLKLWDYFPIRCSAMDEEYGKPHPAVYLSAVKKLGIEK
                     QECIVIEDSVTGLIAAKAANLTTFLVNTDYQNEKFSIADKRIASLLDVIEKLKQH"
     gene            14662..15459
                     /locus_tag="PWY43_000084"
     CDS             14662..15459
                     /locus_tag="PWY43_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor"
                     /protein_id="HDL2135009.1"
                     /translation="MKPFERRQQILAYLAQHGRTEVDVLADYFKLTGATIRKDLTSLE
                     EDKKVLRTYGSVVLIDEEILEREEKINKEDKDDKPLAQKTGINLQQKQAIGQKAAELI
                     NEGNSIIFDAGSTVLQMIPHLKAFDNLSLMTNSLTIFNEILKLNKSYNLLISGGTFRE
                     KSASFHGYFAESAFIGSNFDILFIGTDGLDLDVGLTTFNEVYKVSSLMCDAANKIVLL
                     ADSSKFGRKSPNIVCGLEKIHTIITDEGLSQEMREQIKAKGIELLVV"
     gene            15470..16405
                     /locus_tag="PWY43_000085"
     CDS             15470..16405
                     /locus_tag="PWY43_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDL2135010.1"
                     /translation="MNHLDSAKETLNLYAQQIITLNHRLSEEFNEVVNMILACQGRVV
                     VGGIGKSGLVGKKMVATFASTGTPSFFLHPTEAFHGDLGMLKPIDMVILISNSGETDD
                     VNKLIPSLKNFGNKIIAMTGNPYSTLGRNADVILNIGVEREACLNNLAPTSSTLVTMA
                     LGDALAIALMKARDFRPEDFARYHPGGSLGRKLLNRVRDVMVRKVPTASLDTTFTECL
                     SVMNEGRMGVAVIMQDDKLEGIITDGDIRRTLAKFGAESLNKTADEIMTRNPKTINDT
                     EFLAKAEDLMKELKIHSLIAVNDEGKVTGLMEFSS"
     gene            complement(16449..17002)
                     /locus_tag="PWY43_000086"
                     /pseudo
     CDS             complement(16449..17002)
                     /locus_tag="PWY43_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005752768.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            17304..19163
                     /locus_tag="PWY43_000087"
     CDS             17304..19163
                     /locus_tag="PWY43_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL2135011.1"
                     /translation="MSNLKVKVQGFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPN
                     ETFAKLVGPMITYLLPLLIGYSGGKLVGGERGAVVGAITTMGIIVGSEIPMFLGAMIV
                     GPLGGWAIKTFDKAIDGKVKSGFEMLVNNFSVGIIGMILAMLSFAFIGGIVTQISAAL
                     GAGVGALVEAGLLPVTSIIVEPAKILFLNNAINHGVFTPLGTAQAQEVGKSILFLIEA
                     NPGPGFGILLAYMFFGRGTAKQTAGSASIIHFLGGIHEIYFPYVLMSPRLILAVIAGG
                     ATGVLTNSLLGGGLIAPASPGSIFAILAMTAPGAHFAVVASVILSCAVTFAIASILLK
                     TQKGEASLEDAQAQTQAMKAESKGVVNGNINKIYVACDAGMGSSAMGASMLRKKVQAA
                     GLNIEVQNLAINDLPQDAQLVITHKDLTARATQRVATAQHLSLTNFLDSAFYDSLVAK
                     LANHQPQQAVQNTQNFANPVEEPQAEGLKLQVAQVFLGLQANSKEEAIRFAGEQLVKA
                     GFVQPSYVDAMFAREQMVSTYLGEGLAVPHGTSDAKDSVLKTGIVVCQYPNGVRFTDE
                     EDGVAKLVVGIAAKGNDHIQILSAITNALDDEQAMKTLTSTNDVEKVLALLSK"
     gene            19267..20406
                     /locus_tag="PWY43_000088"
     CDS             19267..20406
                     /locus_tag="PWY43_000088"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2135012.1"
                     /translation="MNALHFGAGNIGRGFIGKLLADARIFVTFADINQTQIDQINQNK
                     QYGVKIVGDDSRVEIVKNIAAINSKDENAVIEQVKNTDLITTAVGPNVLGFIAPLFAK
                     ALVARVESGNTQPLNIIACENMVRGTTFFKGKIFEHLTAEQQAEIEKVVGFVDSAVDR
                     IVPPAEPNPADPLEVTVEEFSEWIVDQTQFKGDIPNIKGMELTDNLMAFVERKLFTLN
                     TGHLICAYLGKQAGVKWIKEAIAIEEIKTQVKATMEESGAVLIKRYGFDPQAHSAYIE
                     KILKRFANPYLNDDVNRVGREPIRKLSENDRLIKPLRGTLEYGLPYQNLVKGVVMALQ
                     YRNEEDPQAVELAEFIANHGVAAAVEKYTGLTDQAVIAQVVELYQ"
     gene            20418..20933
                     /locus_tag="PWY43_000089"
     CDS             20418..20933
                     /locus_tag="PWY43_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HDL2135013.1"
                     /translation="MMDYIDKLSEETTLRGFLSIANQRFAKAVEQLIERVFRKTDFAL
                     KSVVDSLFEHQGPLADIEVRLKVLLGLGVISPKVFEDISLFLEVKQHLNEEEDEFAFS
                     HPAVVKFAKDLHHIDFLPINELLNMKASAANKDSMLFQMQQMRLEKMVRSSLILAVTE
                     IDEQLNVESPL"
     gene            21073..22938
                     /locus_tag="PWY43_000090"
     CDS             21073..22938
                     /locus_tag="PWY43_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDL2135014.1"
                     /translation="MQSLLSFVPDFLKQELFWVMALLVFAIWQFIQNKLRMDVVALIV
                     MLFFSLTNILSVKEVLAGLSDPNVVLIALLFIVGEGLVRTGVANQVSDWLMRVSGASE
                     TKVLVLLMLCIAGLGSFMSSTGIVAIFIPVVLAICSGMGISPRRLMMPLSVAGLISGM
                     MTLIATPPNLVAHSELVKSGFEGFNFFSFTPIGLIILVMGIGYMLIARRWLDNGEQSN
                     THSQAQKFSMSQLIEEYQLKERAKMVLVEADSDCVGKSIGQLNLRIEQGLNIIAIQRT
                     KRFRKITVSASVNEIFQPKDILLLDTALDDETYQQRCEQLKLRPIELKGEYFSSQYRS
                     VGMAEISVMPEAECIGKTIRELKFRSRYHLSIVGLKRGDEIIQEELLDTPLKFGDILL
                     VMGVWQQIHRMDDDHRDFFLLRDPAESKNAPPALSQAPHALFSVLTMVLLMITGIVPN
                     VMAALIACLMLGKFRCVDMKSAYDSIHMPSIVLIVGMMPFSIALQKTGGVDLTVRLLL
                     DAMQGLDVHFVLIVLFVVTAIISAFISNTATAILVMPIAIAIANQLGYSPAPFVMTVA
                     VSASAAFMTPVSSPVNTMVLAPAGYKFIDFVKVGVPFTVLVMLVSVFVIPMLFPL"
     gene            complement(23087..24214)
                     /locus_tag="PWY43_000091"
     CDS             complement(23087..24214)
                     /locus_tag="PWY43_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2135015.1"
                     /translation="MNKLKSINLQVYIMLIAIVVIMSFFSIATDGAYLSARNISNLLR
                     QTSITGILAIGMVFVIISAEIDLSVGSLMGLLGGFAAISNVWWGWPLSLTIIVTLILG
                     VMLGAWSGWWVAYQKVPSFIVTLAGMLAFRGVLVGLTNGTTVSPISNEMTTIGQGYLP
                     DMVGMILGTIGIFCFIVWGSYQRKARQKLNLTVPSMSKDTLKYGLVAIIVLGAIYLLN
                     DYRGVPFPVLVLVILAIAGTFLARKTAFGRHIYAIGGNIDAARLSGISVEKVKLAIFA
                     LNGLLVAIAGLILSSRLGAGAPSAGQNAELDAIAACVIGGASLAGGIGSIYGVVIGAF
                     IIALLDNGMSMLDVPTFWQYIVKGGILLLAVWVDTISKKKA"
     gene            complement(24218..25732)
                     /gene="xylG"
                     /locus_tag="PWY43_000092"
     CDS             complement(24218..25732)
                     /gene="xylG"
                     /locus_tag="PWY43_000092"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDL2135016.1"
                     /translation="MAKLLEMKNITKKFGEVVALNNISISLETGEILSLCGENGSGKS
                     TLMKVLCGIYPAGDYEGEIYFSGEKLIARNIKDTEEKGISIIHQELTLVKNMSILENM
                     FLGNEITKSGITDDNEMYLRCKTLLEQVQLDIDPNTKVGELGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEKETEILLSLIKDLQAHNIACVYISHKLNEVKAISDKICVIRDG
                     EHIGTRSAEGMSEDDIITMMVGREITSLYPHEPHNIGEEVLRVENLTAWHPTNTHIKR
                     VDNANFVLRQGEILGIAGLVGSGRTEMAQCIFGSYQGKYQGDIFLHKQPIKIKNCAQA
                     IENHIVMVPEDRKKHGIIPIMGVGKNITLSSLPQFCFGKKVINEPLEETIINQSIAKL
                     KVKTSSPELAIGRLSGGNQQKAILAKCLLLNPKILILDEPTRGIDVGAKYEIYKLINQ
                     LAKEGMAIIVISSELPEVLGISDRVLVMHQGKIKADLINQDLTQEKVMEAALKE"
     gene            complement(25799..26800)
                     /gene="xylF"
                     /locus_tag="PWY43_000093"
     CDS             complement(25799..26800)
                     /gene="xylF"
                     /locus_tag="PWY43_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2135017.1"
                     /translation="MKLKSKLLAVATATLMVLSNASVAKDLKIGMSIDDLRLERWQKD
                     RDIFVNKAESLGAKVFVQSANGDATAQISQIENMLNKDIDVLVIIPFNGEVLSNVIAE
                     AKKEGVKVLAYDRLINNADVDFYVSFDNEKVGELQAKSIIGQKPEGNYFLMGGSPVDN
                     NAKLFRKGQMKVLQPLIDSGKIKVVGDQWVDSWLAEKALQIMENALTANKNNIDAVVA
                     SNDATAGGAIQALSAQGLSGKVAISGQDADLAAIKRIIAGTQTMTVYKPITNLADKAA
                     EIAVALGKDEKVEMNAQLNNGMKEVPAYLLEPVVVTKENIDDTVIKDGFHTKDAVYK"
     gene            27042..28361
                     /gene="xylA"
                     /locus_tag="PWY43_000094"
     CDS             27042..28361
                     /gene="xylA"
                     /locus_tag="PWY43_000094"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDL2135018.1"
                     /translation="MSNYFDKISKVQYEGANSTNPFAFKHYNPDEVVLGKTMAEHLRL
                     AVCYWHTFCWTGNDMFGVGSLDRSWQKTGDLLQGAKQKAEIAFEFFQKLGIPYYCFHD
                     VDVAPEGNNFKEYQHNFNTIVDILEQKQAETGVKLLWGTANCFTNPRYMSGASTNPNP
                     EVFAWAAAQVFTAMNATKRLGGENYVLWGGREGYETLLNTDLKREREQIGRFMQLVVE
                     HKHKIGFNGTLLIEPKPQEPTKHQYDYDVATVYGFLKQFGLEKEIKVNIEANHATLAG
                     HTFQHEIATATALDIFGSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKAGGFTTG
                     GFNFDAKIRRQSTDPYDLFHAHIGAIDVLALSLKRAAKMLEDQALQKVVDNRYAGWNQ
                     DLGLQILNGKASLEDLAKIVETQGLDPKPVSGQQEYLENLVNSYIYR"
     gene            28514..29959
                     /gene="xylB"
                     /locus_tag="PWY43_000095"
     CDS             28514..29959
                     /gene="xylB"
                     /locus_tag="PWY43_000095"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDL2135019.1"
                     /translation="MYIGIDLGTSGVKVVLLDENQHIIAITQKSLPIFRPHPLWSEQN
                     PQDWWNATNEAVLELSATQDLQAVKAISLTGQMHGATLLDNADNVLSPAILWNDGRSF
                     MECEELEQLVPNSREITGNLMMPGFTAPKLRWVDKHQPDIAEKVSKVLLPKDYLRLLM
                     SGEYASDMSDASGTMWLDVGKRNWNKSLLNACGLDIENMPKLFEGNQITGYLRPTLAE
                     QWKMKSVPIVAGGGDNAAGAIGIGLYQTGQAMLSLGTSGVYFVVSDKFQANPQKAVHS
                     FCHALPDRWHLMSVILSAASAVDWAVKSLGLKDVSTLFQQVEASQTASDAIFLPYLSG
                     ERTPHNDPYAKGVFWGLSHNDNQITMAKAVIEGVSFALAEGIEVLHETGVVADNIALI
                     GGGAKSAYWRQLLADISGKTFEYRTSGDVGPALGAAKLAQIALNPNQDIATFCQPLPL
                     EQVYQPTPARYAEYQQKRQKFTELYRRLKGL"
     gene            complement(30022..30345)
                     /locus_tag="PWY43_000096"
     CDS             complement(30022..30345)
                     /locus_tag="PWY43_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HDL2135020.1"
                     /translation="MNVWILLAISICLEIAATNLLKLSNGFTKAIPTIGSLALYGLSF
                     YFLSIIFRTLPVGIVYAIWSGVGIVLTALVAYFAFGQKIDLAGLIGISLIIAGVLVIN
                     LFSKV"
     gene            30469..31011
                     /locus_tag="PWY43_000097"
     CDS             30469..31011
                     /locus_tag="PWY43_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDL2135021.1"
                     /translation="MAQVYIVHGYTANADKHWFPWLEQELAKCGVSCERLNMPDSAKP
                     SLDGWLNHLAQNVELTDQTVFVGHSLGCIAILNFLAKNYAKIKGAVFVSGFYQPVENL
                     PELSAFTNYYAILPSVSSFPSYVVSALDDKVVNHQYSDALAQHLHADYIRLNQGGHFL
                     DREGVTELPVVLELVKKLLE"
     gene            complement(31182..32006)
                     /gene="dapF"
                     /locus_tag="PWY43_000098"
     CDS             complement(31182..32006)
                     /gene="dapF"
                     /locus_tag="PWY43_000098"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL2135022.1"
                     /translation="MQFSKMHGLGNDFMVVDGVTQNVYLTEEMIRTLSDRHRGIGFDQ
                     LLLVEPPYDPELDFHYRIFNADGSEVSQCGNGARCFARFVTLKGLTNKKDIHVSTAKG
                     KMVLSLQEDGKVRVNMGEPIWEPSQVPFTANKFEKNYILRTELQTVLCGVVSMGNPHC
                     VLQVDDVKTAPVNELGPLLESHERFPERANISFMQVVNRNHVKLRVYERGAGETQACG
                     SGACGTVAVGIMQGLLDNKVQVDLPGGTLIIEWQGVGHPLYMTGDATHIYDGFIKL"
     gene            32155..33477
                     /locus_tag="PWY43_000099"
     CDS             32155..33477
                     /locus_tag="PWY43_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL2135023.1"
                     /translation="MSAMFIIQFAIVLLCILVGARVGGIGLGVFGGLGLAILSFGFGL
                     KPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIATRILRKNPKYITFIAPLVTWTF
                     TVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
                     LEPQGVTLGNVLSVTIPATLLGLGLACVFVNKMGKELKDDPNYQRLLQDPEYVKENHI
                     TTNPTELPLKPTAKLSVGLFLFGALLVVLMGAIPALRPVFDGKPMGMAHTIEIVMLSI
                     GALIILTCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQGHMDEVKSMVSGLVE
                     TAPWAFAFALFVLSVLVNSQGATVATLFPVAIAIGIPAPVLIGVFVAVNGYFFIPNYG
                     PIIASIDFDTTGTTRIGKYIFNHSFMLPGLLSMIFSIGFGLLLSNILL"
     gene            complement(33525..33884)
                     /locus_tag="PWY43_000100"
     CDS             complement(33525..33884)
                     /locus_tag="PWY43_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDL2135024.1"
                     /translation="MISIGAVLGAMCRWQLAVWLNPILSQFAFGTLAANWLGCLLIGI
                     AMGFNLGESQRLLFISGFLGSFTTFSSFSLELTEKLLAEKWGQFVTVLSLHLIGGLLL
                     TLIGVMLARAVTSGRFC"
     gene            34097..34882
                     /locus_tag="PWY43_000101"
     CDS             34097..34882
                     /locus_tag="PWY43_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2135025.1"
                     /translation="MSKSLRNACIGEFIGTGFILFFGAGCVAAAQVAGANFGLWEISI
                     VWGLGVSMAIYISAGISGAHLNPAVTIALAAFYGFEKHKILPYVIAQVAGAFCSVALI
                     YFMYSDLFTAAEAAQGITRGETVGFAGVFSTYPNPNITLVTAFIVEFVITVVLMSTIL
                     AIGDDKNGLPNKALAALLIGLLIAVIGGATGPLTGFAMNPARDFGPKLFAYLAGWGEI
                     ALTGGKEIPYFIIPIVAPICGALFGAWGYKNLIHNNLPANTQE"
     gene            34886..36397
                     /gene="glpK"
                     /locus_tag="PWY43_000102"
     CDS             34886..36397
                     /gene="glpK"
                     /locus_tag="PWY43_000102"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL2135026.1"
                     /translation="MEKQYIIALDQGTTSSRAVLLDKNANMVEVAQREFTQIYPQAGW
                     VEHNPMEIWSSQSSTLNEVVAKAGIKPDSIAAIGITNQRETTIVWEKETGKPVYNAIV
                     WQCRRTSDITDKLKADGHEEYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAERGEL
                     LFGTVDTWLVWKLTQGRVHVTDYTNASRTMLFNIHTKQWDDKMLELLNIPRSMLPEVK
                     NSSEVYGQTNIGGQGGVRIPVAGIAGDQQAALYGHLCVKEGQAKNTYGTGCFMLMHTG
                     NTAIQSENGLLTTIACNAKGEPEYALEGSVFIAGASIQWLRDELKIVHDSFDSEYFAT
                     KEDDSNGVYVVPAFTGLGAPYWDPYARGSIFGLTRGANRNHIVRATLESIAYQTRDVL
                     EAMQSDSKAKLQALRVDGGATANNFLMQFQADILDTKVERPKVKEVTALGAAYLAGIA
                     VGFWKDLEELKDKAEIERTFTPDGDQEKRAKRYKGWKKAVRRSLEWAKEDAEE"
     gene            36549..37091
                     /locus_tag="PWY43_000103"
     CDS             36549..37091
                     /locus_tag="PWY43_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2135027.1"
                     /translation="MSQFQIHTIESAPETAQEALKAVQQANGFIPNLIGVLANAPTAL
                     ETYRTVGGINSRNSLTAEEREVVQITAAVVNGCGFCVAGHTKIALKALKMPEEVVNAL
                     RATARIDDNKLDTLARFTLAVMLQKAKLTEAQLNEFFAAGYNQQNAIDVILGVSLATL
                     CNYVNNIAETPINPELQPFA"
     gene            complement(37202..38260)
                     /locus_tag="PWY43_000104"
     CDS             complement(37202..38260)
                     /locus_tag="PWY43_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL2135028.1"
                     /translation="MSVLSSSFIQWLQQNAENLDQSNERADLLIPQIAALGLYKIGVP
                     ETLGGSGGTLVDAIKAVTELGNHSLAAAFVSWGHRTFIEHIIASKNPIPRQSYLADLL
                     NGNLTAGTGLSNAMKFLSGIEELNVNIREENGKRYLNGRLPWVTNLRSYRFVASFVAG
                     FQDERKPLVIAVPSTAEGLTRTKELEFMSLQGTNTTALVFDNVELKEEWILSDNAPEF
                     LATTRPAFLGLQFGLPFGLVEKSLSEIEKSLSFRAILTSEWQSQVDALNDIKNRLFNG
                     LNEEGYFVRNPKELFQARIDIVNVVAQTILLELQASGGRGYLKNDTSGFSRRWKEAAF
                     LPVVTPSAVQLRMVLANS"
     gene            complement(38415..39158)
                     /locus_tag="PWY43_000105"
     CDS             complement(38415..39158)
                     /locus_tag="PWY43_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2135029.1"
                     /translation="MKQKYIYGLAGLLLLLLGWQLGTALLSQRLPMASMLAPAKTWES
                     FTWLISSGEIFPHLWASLQRIAVGLLAALVIGVPLGLLLGFSKHLEMLCTPSFQLLRM
                     ISPLSWMPIIVMLLGVGDLPIYFLLAFATVWSLIISTTSGVKSIDPQWLQLGRVMSAT
                     RKELLCKIVVPAITGHVLTGLRVAVGIAWVVLVPCEMLGVNQGLGYFILDTRDRLAYS
                     ELMAVIIIIGLIGWALDSLIRLALAKGGR"
     gene            complement(39175..40341)
                     /locus_tag="PWY43_000106"
     CDS             complement(39175..40341)
                     /locus_tag="PWY43_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2135030.1"
                     /translation="MDQQRRDFMKLLSLFSLAGSLPLIQACEQVQKNAENRPLVIGYL
                     PITDATPLLVAHAKGLFEKHGVQVEKPVMFRSWAQLVEAFLSGNVNVVHLLSPMSLWA
                     KYGANAPVKAVMWNHLAGSALTVRPEINSIAELSGKTVAIPFWYSIHNIVLQRLLREN
                     GLKITEKEPNSGEVRLSVMAPSDMVAALASEAISGFIVAEPFNALAEAKGVGKVLRFS
                     GDVWKDHACCLTLMHEYDIQHRPDWVQKVVNALTEAQVYTLENRAETAKLLARGSGYT
                     PHDQKVLEKVLAPTQAQWDKYIETGAIQNPQWHQQRIGFQPYPFDSYMEELVKMLKQT
                     HIAGNNAFLQQLDPVEAAKAINEPTFVRKAIEQGGWVETFNLQAGWTRTEQISV"
     gene            complement(40352..41143)
                     /locus_tag="PWY43_000107"
     CDS             complement(40352..41143)
                     /locus_tag="PWY43_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2135031.1"
                     /translation="MSLSITNLSLGYRKNQQTKTILHDVTFNVESGTLTSILGPSGVG
                     KSSLLRVISGLDKPLSGKVALFGEKINQPHPDVGFVFQSATLLPWLSVRNNVAFGLDF
                     QCRLPLSKADIQHRVESALEEVGLREAIDLMPHELSGGMAQRVNLARALARQPKVILL
                     DEPFSALDEVIRSQMQTLLHDIVRHHNAAAIMVTHDIDEALLVSDDVLLLGGDTAHIV
                     GKWHLSAPFPRTDLLSLNPIRVEILHTLRQAQQRAVQEQTVDFVI"
     gene            complement(41338..42774)
                     /locus_tag="PWY43_000108"
     CDS             complement(41338..42774)
                     /locus_tag="PWY43_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_140441352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDL2135032.1"
                     /translation="MKIWLSGIYEFFLRYKNIWAEVWKIRKELDHPNRKKDESEFLPA
                     HLELIETPVSKKPRLIAYLIMLFLVVAIVLASVSKVEIVATAPGKLTFSGRSKEIKPI
                     ENAIVQEIFVKDGQFVEKGQLLVSLTALGSDADIKKTMASLSLAKLENYRYQTLLTAI
                     EKESLPVIDLSRTEFKDSSEEDRLRIKHLIEEQYTTWQKQKTQKTLAYKRKEAEKQTI
                     FAYVRKYEGATRIEQEKLKDFKALYKQKSLSKHELLAQENKLIEAQNELAVYRSKLNE
                     LENDLLNVKEELELITQFFKSDVLEKLKQHIENERQLRLELEKNNQRRQASMIRAPVS
                     GTVQQLKIHTIGGVVTTAETLMIIVPEDDVLEATALVPNKDIGFVAAGQEVIIKVETF
                     PYTRYGYLTGRIKHISPDAIEQPNVGLVFNATIAIDRKNLTSPDGRKIDLSSGMTITA
                     EIKTGERSVMSYLLSPLEESVTESLRER"
     gene            complement(42786..>43466)
                     /locus_tag="PWY43_000109"
     CDS             complement(42786..>43466)
                     /locus_tag="PWY43_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2135033.1"
                     /translation="PTILNNVNLEIRQGEVIGIVGRSGSGKSTLTKLLQRFYIPENGQ
                     VLIDGHDLALADPNWLRRQIGVVLQDNVLLNRSIRENIALSDPGMPMERVIYAAKLAG
                     AHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNNPKILIFDEATSALDYESE
                     HIIMQNMQKICQGRTVILIAHRLSTVKNADRIIVMEKGEIVEQGKHHELLQNSNGLYS
                     YLHQLQLN"
BASE COUNT        12534 a         9061 c         9205 g        12666 t
ORIGIN      
        1 tacgcattgc tacgtccaca cctttgcgga tcagcggttc tccacctttg gcgatcaggc
       61 gtgaaagtgc tgatgagagt gagctttcgc tgtgggttga aaccaatttg ccggtaatca
      121 ccaagcccca cgccgctgca ttcacaaaca gagaggggct gttgccggtg tgtgaacgcc
      181 aatccccttt ggcaattttg tctcgaatta atttatccgc agtggcggtg tcaggaatgc
      241 gtagtaatgc ttcggcaagg cacatcaacg ccaccccctc ttggctggag agtgaaaatt
      301 cgtgcattaa cgcatcaacc ccgcttgctt tctggcgtgc agttcggact ttaccgacta
      361 attgggtagt gagcttttta atgttatcag cctgttcatc acaatgtgct tgttctatca
      421 gattttgcac cagggtttgc tcatcaaagc ggtaatgacg gctcaattca ggacggatag
      481 tggtgttagg taaaagttgg tatgacatca tttttccttt ctatacagca agcgatcaaa
      541 tttctgtata aacttgcaaa ttttaagata aaaccgaccg cttgttattt aacttaacct
      601 attgttatta aatactattt agataaatta tggacaacaa ccacgttttg gcagagtaat
      661 ttcctcaggt tctttggcta attcttccgg tggcaatcgc tcaattttga tattcaaggt
      721 tgccaagcca atcgcaatca gtggaatgag ccaattcatt attgcatacg gtgcatattc
      781 aaaagcactc acttgcaaca ttgagaacgc ataaacacca caggtattcc aaggtacaag
      841 aaccgaagta actgttcctc cgtcttccaa tgcacgagaa aggtttctcg aacttagtcc
      901 cttatcccta taggctttta tatacattct gcctggtaat aaaatagaaa tatattgctc
      961 agatgtcgtt acgttggtaa taaatgccga tacaatcgta gcactaatta agctacctgc
     1021 tgattttgca agggtaagga ttttttccac tactgaatgt aacattccag cttgctctgc
     1081 aatgccacca aaagtcatcg caacaatcgt aagggaaatg gtgtacatca tcgactcgat
     1141 cccaccacgg ttgaacagct tatcaatcat ctcgtggccg ctctcaattt tatagccgtc
     1201 gtgcagagta atcactgcta cgctgacatc accgccttgc acaaaaacgt ggcagagcca
     1261 accaagaatt atccctgcag ctaatgccgg taaggcaggc actttttttg ccaccaacac
     1321 cacgactaat aaaggcacta atagcaacca aggcgaaatc aagaaatgtt cctgcattgc
     1381 ctgcataatg tcttcaattt tagcaagatc gagattttcg gctctaaatt ccataccaaa
     1441 atagaaatag acagataacg caatcaggaa cgccggcacg gtagtgataa acatatggcg
     1501 gatatgttca aacagattgg tttcggtaat ccctgctgcc agattggtag tgtctgacag
     1561 tggcgacatt ttatcgccga aatatgctcc tgagatcacc gcaccggcaa ccatcggtgc
     1621 cggaatgccg agactaaacc cgatccccat cgccgctata ccgattgttc ccattgtaga
     1681 ccacgagcta ccaatcgcaa gggtcacaat agcacaaatt attgagatag ttactaaaaa
     1741 cagcgacgga gagatcaatt tcaagccgta gtaaatcatc gtagctacaa ttccaccacc
     1801 tagccaagct ccgatggtta gcccaatcat aataatgata acaatcgctg gtaaagccat
     1861 acgaatgcct ctataaattc ctttttcaat tgtctcccaa cggtagccat gtgccatcgc
     1921 aatcacagcg gcactaattg ccccaataag caacggaacg tgaggtgagg cttcaaattt
     1981 gatgatagcc accgccataa tcgataccat aataatcaca ggcaatatag catagcgaaa
     2041 cgggattcgg atattttggt ctgttatttc tgttctgttt gacataaagc caccttttac
     2101 aagcggttga tttttggaaa aagtttgcaa aatcagcatt ttacaactgt tttgcttcct
     2161 gccaaaaagg gttaaaggcg atttggtagg ataaaatata gagaaaatga caattaagcc
     2221 aacacctccc gaattttctc caatgcccaa tccaaatcga atttttcaat caccaaaggg
     2281 ggagcaaagc ggataactgt atcgtgggtc tctttgcaga gtaagccttt ttctttgagt
     2341 ttttcgcaga atgggcgtgc tggttgatgt aactccaagc caataaacag tccacgtcca
     2401 cgaacttctt taataattgg attttggatt tttttcagct ctgccaagaa gtattcacct
     2461 aactcacggg aacgctctgc aagtttttct tccaacagta catcaattga ggcaatagat
     2521 accgcacagg ctaatggatt accaccaaat gtcgaaccgt gcgagccagg attgaatacg
     2581 cctaaaatat cacgatttgc taacacacaa gagattggga acaccccgcc accaagtgct
     2641 ttacctagaa tataaatatc tggctcaaag ccttcccatt ctgttgcaaa taacttgcca
     2701 gtacgcccaa gacctgcctg aatttcatca gcaataaaca gcacattatt ctctttgcat
     2761 agttcataag cctgttccaa gaaaccttca ggtggaataa gaatgccagc ttcaccttgg
     2821 attggctcaa ccaaaaacgc tgctgtgttt ggtgtaatcg cagcttttaa tgcttctaaa
     2881 tcaccgtatg gaacaagttt gatgccacct aacatcggcc cgaaaccacg tttataatcc
     2941 ggatcagacg agagagatac tgcagccatt gtacgtccgt gaaaattgcc aacacaagcg
     3001 atgatttctg cttgattttc tgcaattcct ttcacatcat aaccccaacg gcgagcagtt
     3061 ttaatcgctg tttctaccgc ttctgcaccc gtattcatcg gtaatgccat ctctttatta
     3121 gcgagtttgc aaattttttc ataccaaata ccaagttgat cgttataaaa tgctcgagag
     3181 gtcagcgtta ctttgtctgc ttgatctttt agtgcctgta caatttttgg atgacaatgc
     3241 ccttgattaa ccgctgagta ggcactaagc atatccaaat agcgattacc ctcaggatct
     3301 tcaacccaaa tgcctttagc tttactaata catataggta gaggtaggta gttattggca
     3361 ccgaaatgtt cggtttgttg gatgagttgt tgagaagtag acataaaaaa ctcctttatg
     3421 ttaaaaactt gtaacacaaa agagtttaac aatctttagc taaaaaacac ggactttttg
     3481 ttgattttat gatctacttc tcatttttct tcagttgttg attaactatg caattaatat
     3541 gaatagatag catactttaa gcaaaaatgc ctccattttc tataactttt ataagtttat
     3601 agataacttt ctccgttgaa attccaacta taaaatttag ggctgtccta gataactaac
     3661 ttaaacaacc cctaaccaat tgttttaaaa tggaaatttg agattttaag tcactgtgat
     3721 taaaacgcta ttcgcattcc ttcaaataca gctcaaaatg agctttaggt atgccgttaa
     3781 atttacgcaa atggcgtttt gcttgactcc aaaaattctc aattccgtta atgtgatttt
     3841 gattttctgc gaagtgtttg ctgtgattga tacgaaaatg gttaaattca ctcacatcaa
     3901 gcacatcata gcttcgataa aaatcggtgt aaacaaggct atcaggcttc actttcctcc
     3961 cgaataatcg gcaaaagtgt ggcagattgc gtatttggta cgacaacggt ataaaccttg
     4021 tcatcacgtt ttaacagccc aaatacggca gttttgcctg cggcaccaca ccctcgttta
     4081 cctttgcgag caccaccgaa ataactttcg tccgcttcaa tttcaccctc aaacatttcc
     4141 agatgagggc tatgttgata gatgagcaat cgcaaacggt gaaagtaata ggcagcggtg
     4201 gttttgttta cattcaccaa ctcagccgct gttcgagcag tgacacctgc tacaaacagt
     4261 tcaatgagtt tatgttgttt gtactgactt agacgacttt ttctcattgg gttatcttaa
     4321 cctaaatgga tgttttagtc gttatctagg acagcccctt attttttgta tgtagtttga
     4381 ctgctgcaag cggtgtgatt ttgctatttt tttgcaaatt tagcctgtat tttatgagca
     4441 acaataaaca gcactaagac tccgatgata attggatcga atgttacgct ctttaccgac
     4501 caataataat gtaccatcgc caatactaat gcaggataag cccactggtg aatcgtcccc
     4561 cagcgtgttc ctaattttcg ttttagcata ggcagcgaag ttattgccat tatgcttaga
     4621 atgaaaaaag cagctgctcc taaaatcaaa tatgggcgac cggcaagctc gctaaaaaat
     4681 aattttacat caaggcttaa ttctaacaat agatagcttg aaatatgtag agctgcccat
     4741 acaaacgccc ataagcccaa tggacggcgt aaaatttgat actggttttt ctttaggtat
     4801 tgtagggcaa taccaagtag aaacattata caaaaaatga cgattgcacc ataaccgaga
     4861 aaatgctcca gctctttaat cggatcggca cctaatattg tttcattacc cgtatataat
     4921 agctgtgcta cccaaactag cggtaataaa cagccaagat gaatgagtat acgtaaccag
     4981 gttagcatta gtagttcacc tttaaatcta agccacggta taaatttgcc acttcatttt
     5041 cgtagccatt aaacattagt gttggttggc gtttgacgga taataacccg cctgcaccaa
     5101 ttactcgttc agatgcttgc gaccaacgtg gatgatcaac atttggattc acattagcat
     5161 aaaagccata ttcatttgga gcaagacttt cccaagtagt gagtgggcgt ttttcagcaa
     5221 aggtgatttt cacaatcgac ttaatacttt taaagccata tttccaaggc acgactaagc
     5281 gaataggagc accgttttgc ggtggcaggg ttttaccgta taaaccgacg gatagtagtg
     5341 ttaatgaatt taaggcttca tctaagcgta aagcttctac ataaggataa tcaatcccac
     5401 cgcctagcaa gcgatttttt tgccccggca tttgtgcagg atcatataaa gtgtgaaaga
     5461 ccacatattt ggctttactg gttggttttg ccatctcaat taagcgagct aactcaaagc
     5521 cgatccacgg cacaaccatt gaccacgctt caacacagcg aaaacggtaa atgcgttctt
     5581 ctagtggaaa cgtattaagc aattgttgat gatttaagct aaacggattt tcaacttctc
     5641 cgccaatttc caatgtccaa ggatcggttt tgaagtttgc cgcatttttt gctggtgcac
     5701 ttttatccac gccaaactca tagaaattgt tatagcctat tactttgttt tccggagtga
     5761 gttcaagccc tttatttgga tccggtttaa actgtagtga ttgcaagtgt ctttgtgttt
     5821 ctgccatact tactttaggc aatgcagaag ctgcaatacc taaacctaac gcggtaataa
     5881 ttttacggcg ttggtagaaa atagtttcgg gtgtaacatc ttgaattgtt aattttttca
     5941 tcgaactatc ctttttttat gaattgttgt tattattttg gactcagctg atctagaatt
     6001 cttacaaaat aagtgtaatt ttttattggg tattccctaa aggagatata tgaagagtct
     6061 atcttctaca cagatagaag agattagaaa gcaaatggtt aaatttgcca ccctacaact
     6121 agccgataaa gatttagctg aggatgttgt acaggatgcc ttaaccaatg cctataagca
     6181 ttcagcctct tttcggcgag aagctgcact taaaacttgg atttttgcta ttttaaagaa
     6241 caaaatttta gatttactca gaacacgaaa acgacatatt cctgtcagtt ctctatgtga
     6301 ggaagaagaa tcaccaagtg cctttttcga tcgccacgat cattggttgg aacatcaaga
     6361 tgtaactgaa tggaggggga tagctcaatc aacttaccaa aaagaatttt gggcaatttt
     6421 tgatatttgt ttaaataaat tacccgccca gcaagcacga gtatttatga tgcgtgaaca
     6481 tttggagatg agtagtgcgg aaatttgcca agaatgtgaa atttctctct ctaacttaaa
     6541 tgtattactt catcgtgctc gtttacgttt acaagagtgt ttatctcgta attggtttag
     6601 agaggaatca aaatgaaatg caggcaagca acagaactta tttcacagtc ttgtgatgag
     6661 aaacttcgtt tgcaacaaca tatgcgtttg cagttgcatc ttttggtatg ccctaaatgc
     6721 cgaaatttta ataaaaatac caagaaatta ggcaagatat taagagctta ctgtaacgat
     6781 aaagatgctc cgttctgatt tattatacat tttaaaagga gaaaaatatg cgttattggt
     6841 taataagttt aagcatacta ttaagtggct gtgtcgtgcc ggttactcct acggggcaag
     6901 ccggtatccc tatgccgatt ataattggtg tctcagatcc acttgattcc aaagaggttc
     6961 acatttgtac tctaaagcca tttactcagg aatatcgtag tgaacatagt agccgaggca
     7021 gagccaaatt aaatgtacaa aagcaatgcc gtgcaaacca cagtgaaatg ttttgtgaag
     7081 aaaaagatat tgtttgcaaa acctatgatt agttaaacaa gcggtcgtat tttatctgtt
     7141 ttttgcaaaa ttttcctaaa atttgaccgc ttgtttattt aagcagataa catcaaaatt
     7201 ttatgaggat tgtctatgca acccatcaat attgccatcg caggtgccgg cttttccagc
     7261 cgagtttttc atatcccgtt tttgaaacaa gatgctcgtt ttaatattgt aaaggtatat
     7321 gaacgaacct ctaacaatgc ggcagaatgg ttgccgaatg cagaaacagt gcgggatttc
     7381 aacgctcttt taacgccaga agtggattta gtgctgatta ccacaccgaa tcaaacccat
     7441 tatgcaatgg taaaagagtc attgcttgca ggcaaacacg ttttagttga aaaaccgtta
     7501 gtggcaacag tggctgaggc ggaagagttg gcggaacttg caaagcggca aggaaaggtg
     7561 ctttatgtgt accaaaaccg ccgttgggac agccatattg cgacggcaaa atcgctaatg
     7621 gagcaaaatt tactgggaga gttggtagat tgcgaaattc gttttgaccg ttatgcgaaa
     7681 gagaaaaatg ttaaagtatg gaaagaatca ggcgagcttg gtacaggttt agtgtatgat
     7741 ttaggcgtac atttaattga ccaagctttg tatcttttcg ggaagccgga ggcggtgtat
     7801 gctgatgtgc gttatcagca cgaaggggca ttggttgatg ataactttga tattcagctc
     7861 tattatgcta acggttttaa agtggtgctg aaagccagcc gatatgctcg tgaaccaagc
     7921 ccgacatttg tattacacgg aaaattaggc tcttatctga aacaaactcc cgataatcaa
     7981 gaagatttgc tgaaaaatgg cgtacaacct attggcaaag attggaatat cgaagaaaag
     8041 gagaactggg gaattttaca cactgaagtg gaaggtaatg tggttcgcca gccctatcga
     8101 aatgccgaag gcagttatca aaacttgttt gatgatttgt accaagccat cgctcacaat
     8161 gctgagccaa ttatcaaact cgaagacgtc atttttgtgc taaaagtgat cgaaagcgta
     8221 tttgaaagtg caaaattagg gcaaaaaatc tatttgtaaa attttgagga tttttgaccg
     8281 attgttattc tctctgatgg cgcaagcgtt tacgcttgtg cctattgatt attttagctt
     8341 tgtttctagt acaagcgtgg acgcttgcac tatctaaaaa atcatctccc ccattgcttt
     8401 tcccatctcg attggctaat atgcaacctc aatccgtagt tagaatactt aatatgttaa
     8461 gttttaactg ttttttcact ctaataagga tcatttcaat gaataaactt attgcctctt
     8521 tggtggcttc aagctgtgcg acggcttttg ctgctcctgt tcaacagaac aatttccaca
     8581 ccactaccca cacttacgaa tttacgcaat cttatgattt agttgtgcca caaggctcga
     8641 aaggaacaac taatttgtgg attccgttgc cgtctgatac cgattatcaa acggtaaaat
     8701 cgatagagtt tgagggaagt tacaaaaacg cctatatcac agaaaataac gcttatggtg
     8761 caaaaacctt gtttgccact tgggatactg atgcaccaaa gcgtgatcta aaagtgaaaa
     8821 tggtgatcca aacccaagat cgtgagccta tggctcaagg ggcgttggca aattaccaaa
     8881 tgccggagaa aatcacttat tctgttgatg tattggagta tttaaaacca acggctcaca
     8941 ttaaaacaga cggtattgtg aaagaatatg ccgataaaat tgtgggtaac gagcaaaatc
     9001 cgttgaaaaa agcggaactt atccacaagt ggattgttga taatatggaa cgtgataact
     9061 cggtgctagg ctgtggcgat ggcgatgtgg agaaaattct caccaccaaa gtgctaaaag
     9121 gtaaatgtac cgacatcaac tcggtgtttg tagcacttgc tcgtgcctct aacattcctg
     9181 ctcgtgaagt gtttggtatt cgtttagggc aagcggtaaa aatggggcaa tattccaaaa
     9241 ccgcatttgg tagctcaaaa gatggcgtgg cgaatgaaag cggcgggcag cactgccgtg
     9301 cagagttcta tttagcaggc ttcggctggg tgcctgtaga ttctgccgat gtcgctaaaa
     9361 tgcgtttagc cgagaaaaaa gacgtggatg atgctgatgt tcaagcggtc tcgaaatact
     9421 tattcggcaa ctgggaaatg aactgggtag gcttcaatca cggacgtgat ttcgatttat
     9481 atccacaacc ggaacttaca ccactgaata actttggcta cccttatgct gaggtaggtg
     9541 gtgacccgtt aaactcgttt gaggcgaaag aatttggcta tgaattcatc tctaaagagt
     9601 tataaccata aatttatcac aatgtgcgtg accgccatag tgtcggcggt gacctccaca
     9661 ttctgttgta ttggaccact ggtttattta gtgtttggca tctcttcccc ttggttgatg
     9721 gagctaaacc aactggagtt tctacaactg ccgatgttgc tgatttcgct cgctgcgttt
     9781 ggctatggct tctggttgct gaatttttcc aacaaaatca tctgcaccaa atacttctcc
     9841 cgcagaaccc tggtcatttt gtattgggta gtttctgtgg tggtgatgtt ctttttaact
     9901 tacccttatg tattgcctta cattttggaa atgtggagag gagaataaaa tgaaaaaata
     9961 tatcgcaacg atgctgttgg cttttggttt ggttcattca agctacgcac aagaaaaaac
    10021 agctcaaatt gaaacgaaac aagcagagaa aaccgtcgtc ctccatattc cggaaatgta
    10081 ctgccagctc tgcgtttatt tagtcaataa agaactacga gcagtagaag gtgtgatttc
    10141 caccaaagcc tcaatgaaag agcgaaaagc gactgtggtg gtaaaagaaa gtgtggatga
    10201 ccaagccctg atacaagcgg tcggaaacct caaatatact gcaaaagtca ttgaataaaa
    10261 ccaaagctca aacattattg tttgagcttt tttaatttag ccttcctttt ttccccatct
    10321 aatattcaaa cttcactttt gttttgattt aaaatgaaaa aataatttat tttgaaagtt
    10381 gtttctcatt ttgtgatcgc tgtcacagtt tttaaaataa aaataattta ctatttgcac
    10441 atctcataac agagatgaaa gaaaaatatt ttattttgaa aaacggagat cggtatgatt
    10501 gaagcaatta cacacggagc agaatggttt atcggcctgt tccaaaaagg cggtgaaacc
    10561 ttagtcggta tgataaacgg tattttgcca ctcttaattt cattattggt tgtaatgaat
    10621 gccctgatta aatttgtagg gcaagatcgg attgaacgac ttgctcaacg tagtgccgga
    10681 aaccctattt cacgttattt gatcttaccg gtgatcggca ctttcgtttt ctgtaacccg
    10741 atgacattaa gcttaggacg tttcttaccg gagcgttaca aaccaagcta ctacgccgca
    10801 gcctcttata gttgccactc tatgaacggc ttattccctc acatcaaccc gggtgaatta
    10861 ttcgtttatt taggtattgc cagcggttta accaccttag gcttaccact tgggccttta
    10921 gccatcagtt atttattagt cggtatgttt accaatttct tccgtggctg gataacagac
    10981 ttcaccaccg cattctttga aaaacgaatg gggatcacct tagatagaga agtacattta
    11041 tcccactaac caagtaactg aaatacagag gaatagatta tgagcaacaa agcgatttac
    11101 attgaaaaag gcaacggtgg ttggggtggt ccattaacat tacctgttgt ggaaggcaaa
    11161 aaagtgcttt atatgaccgg cggcactcgc cctgcgattg ttaataaatt ggtggaatta
    11221 accggctggg aagcggttga tggttttaaa gaaggcgaac cgctggcaga agaaatcggg
    11281 attgcgatta ttgattgcgg tggggtatta cgctgtgggc tttacccaaa acgccgtatt
    11341 ccaaccatta acattcactc caccggtaaa tccggccctt tagcagaatt tattgtggaa
    11401 gatatttatg tttcagcggt gaaagaaaac aatattcgct taattgatgc cgatggaagt
    11461 gtgcctgtgg caacagagca agcaaaacca acttcatcaa cgacagcaac ggcaaaagag
    11521 tatgactcca gcaaaaaaat caccgaacaa agtgatggtt tattagcaaa aatcggtatg
    11581 ggaatgggat cggtggcggc gttattcttc caagccggac gagacactat tgatacggtg
    11641 cttaaaacca ttttaccgtt tatggcattt gtttctgcgt taatcgggat catcattgcg
    11701 tcaggtttag gtgatgtgat tgcccacggc ttgacccctc tggcaaacag cccaatcggt
    11761 ttagttacac tggcgttgat ttgttccttc cctctccttt ccccattctt agggccggga
    11821 accgtgattg cacaagttat cggcgtgtta gtcggtacgc aaatcggttt aggcaatatt
    11881 ccgccacatt tagcactgcc ggcactcttt gctattaatg ctcaagccgc ttgtgatttt
    11941 attcctgtgg gtctttcaat ggcggaagca aaacaagata ccgtacgggt gggtgtaccg
    12001 tcagtattag tcagccgttt cttaaccggt gcaccaaccg tgctgattgc gtgggcagtt
    12061 tccgcattca tctaccaata attaaccacg atacaagcgg tggaatttga aaaatttttt
    12121 gcaaaatgca ccgcttgtca aaacgagaga acatttatat gacgattatt tatcaaacga
    12181 cctttaccaa aatcgggaat tttgctcagg aatcattaac agacgatatg ttaatcactt
    12241 ttaaacaagg cgcaccggca gatttagaag attactgctt cattcataat ccatctgaat
    12301 taagtagccc tttagaagta ggcgatatcg ctgaatttga tggcgtagct tatccgatta
    12361 cggcagttgg tagcgttgca tctgaaaact tatccgccct cggacacatt accttccgtt
    12421 ttgacggggc aaatgatgcg gaatttccgg gaagcgttca cgttatcgga acaccgccgc
    12481 aaggcttaac agagaactca accttgatta tcaagcgtga ttaatgacgg attaaaaata
    12541 atttcaaatt gaaaatttag aggaatacat tatgaacaat gaaaaaaaag tcgctatcgt
    12601 tgttggcggc ggtcaaacac ttggagcttt tttatctgaa ggcttggcag atgccggtta
    12661 tcgtgtggtg gttgctgact caaacggcga aaatgcacaa aaagtggcag ccgacattaa
    12721 tgccaaacaa ggcgaaggaa atgcgattgg ggtgcaagtc gatgcggctg atgaaccaag
    12781 cgttgaagca ctagctaaag caacagatac cgtattcggg cgtgccgatg tattggttta
    12841 cagtgcaggc acagcgaaag catcgcctat ttgggatttc aacttaaacg attttgattt
    12901 gtcggtaaaa gtgaatttaa ccggttattt cttaaccgca aaacacttct cccgcttaat
    12961 gattcgtgac ggtattaaag ggcgtatcat tcaaattaat tctaaatcgg gtaaagtagg
    13021 gagtaagtat aattccggtt atagtgcagc gaaatttggt ggtgttggtt taacacaatc
    13081 gttagcatta gacttggcag ataaaggcat taccgtgcat tcattaatgt taggcaattt
    13141 attgaaatcg ccgatgttcc aatcgttaat tccgcaatat gcggcaaaac tcggcattcc
    13201 ggaaagcgaa gtagagcaag tttatattga taaagtgcct ttaaaacgtg gctgtgatta
    13261 tcaagatgtg ttgaatgtct taaaattcta tgccagcgaa gaggcagctt attgcacagg
    13321 acagtctatt aatattaccg gtggtcaggt aatgttctaa gcaaacaagc ggtcaaattt
    13381 gtcgattttt ttacaacaat ttgcaaaaaa tgactaaaat aaaccgcttg ttaattcgcc
    13441 acaccggtta atatgccaca ggggaaagcg tattgttttg aaatcgcagg tacgtttagg
    13501 ctctgtggct ttttattaag gagcaatgat ggattcaatt acacttctga ttatattggc
    13561 aatgataatg tggctgttac aagccgtttt gggttggctt caaattaaag cctttaacca
    13621 agcctttatg gaaatcagta agaaaggcaa agtaacggtt gggcgtaata gtggtcgttt
    13681 taatcctaaa tcggttattg tgttggcgtt tgatgatgag aaaaaagtgg tagatagcct
    13741 ttgtatgaaa ggattttccg tctttgccag accgcagaaa ttagcgtctg tgattggttt
    13801 gtctcttgat caaattgtgc cggaaaaaat ctttccaagc gataaacgtt cacaatttgc
    13861 cttaaaagtg gcgatatctt ccggatatag ttgctcagat caaaaataat gcgatttttt
    13921 atttcatttt gaatattttt ttagttaaaa atgagataat ggagaataaa tatgcagatc
    13981 aatagtgtca tctttgatat ggacggggta atgattgatt cagaacccca atgggcagag
    14041 gctcaaattg cagtattagc cgacttaggt attaatatca ccgagcaaga ttgcgaacaa
    14101 ctcaccagag gtaagcggat tgatgagttg agcgacactt ggattaaaaa atttcagtta
    14161 tctgtttcca accaagcatt aagtgaagcc attttggcac acggttgtgc tgcgattgaa
    14221 caacacggca aagcattaac gggtttatat gaattattgg atttcttaaa atctaatcag
    14281 atcaagttag ccgttgcaac ctcatcgccg ccggtggtga ttaaagcggt gtttgatcgc
    14341 ttaaaactgt gggattattt cccgattcga tgcagtgcaa tggacgaaga atatggcaag
    14401 ccacaccctg cggtctattt gagtgcagtg aaaaaattag gcattgaaaa acaagagtgt
    14461 attgttattg aggacagtgt aaccggctta attgccgcca aagccgctaa tttaactaca
    14521 tttttggtga ataccgatta tcaaaatgaa aaattttcaa ttgccgataa aagaatagca
    14581 tcgctactag atgtcattga aaaattaaaa cagcattagc acagattagc tcgctccaat
    14641 ctttgctaaa ttggaggaac aatgaaacct tttgaaagaa gacagcaaat tttagcctat
    14701 ttagctcagc acggtagaac cgaagtggac gtgctggcag actattttaa gctaactggt
    14761 gcaaccattc gtaaagattt aacttcactt gaagaagata agaaagtctt gcgtacttat
    14821 ggcagtgtag tcttaattga tgaagaaatt cttgaacggg aagagaaaat caataaagag
    14881 gacaaagacg ataaaccgct tgcacagaaa accggcatta atcttcaaca aaagcaagct
    14941 atcgggcaaa aagctgcaga gttaattaat gagggaaact ctattatttt tgatgcaggc
    15001 agcacagtgt tacaaatgat cccccattta aaagcatttg ataacctctc tttaatgaca
    15061 aatagtttaa ctattttcaa tgagattttg aaactcaata aatcctataa cttactgatt
    15121 tccggtggta catttcgtga gaaatcagct tcatttcacg gttattttgc tgaatccgct
    15181 tttattggtt caaattttga tatactcttt attggcacag acggactaga tttagacgtt
    15241 ggattaacga ctttcaatga agtatataaa gtcagttcgc taatgtgtga tgcggcaaat
    15301 aaaatcgtct tacttgccga ctccagtaag tttggacgta aaagtccgaa tattgtgtgt
    15361 gggttagaaa aaattcatac tatcattacc gatgaaggat tatctcaaga aatgagagaa
    15421 cagattaaag cgaaagggat tgagctgtta gttgtataag ggcgtaaaaa tgaatcattt
    15481 agacagtgca aaagaaacgt taaacctcta tgctcagcag attattacgc taaaccatcg
    15541 actttctgaa gaatttaatg aggtggtaaa tatgatactc gcttgtcaag ggcgagtggt
    15601 tgttggcggt attggaaaat cggggctggt tggcaaaaaa atggtggcaa cttttgcctc
    15661 caccggtaca ccgagctttt tcttgcaccc gacagaggct tttcacggcg atttgggaat
    15721 gctgaaaccg attgatatgg taattctgat ttctaacagt ggtgaaactg atgatgtgaa
    15781 taaactgatt ccaagcctga aaaatttcgg caataaaatt attgcgatga caggtaatcc
    15841 ttactccaca cttgggcgaa atgccgatgt gattttaaat attggtgtag agcgtgaagc
    15901 ctgcctgaat aatctagctc caaccagctc tactttggta acgatggctt taggcgatgc
    15961 gttagccatt gcgctaatga aagcccgtga tttccgccct gaagattttg cccgctacca
    16021 cccgggtggc agtcttggtc gtaaattatt gaacagagtg cgagatgtga tggtgcgtaa
    16081 agtgccaacc gccagcttag acaccacctt taccgaatgt ttatctgtga tgaacgaagg
    16141 gcgtatgggg gtggcggtaa ttatgcaaga cgataaattg gaaggcatta ttaccgatgg
    16201 tgatatccgc cgcacattag cgaaattcgg ggcggagagc ctaaataaaa ccgctgacga
    16261 aattatgacc cgtaatccaa aaaccattaa cgataccgaa tttttagcca aagccgaaga
    16321 tttaatgaaa gagctgaaaa ttcactcatt aattgcagtg aatgatgagg gtaaagtaac
    16381 gggattgatg gagttctcta gttaatttat agatgtaacg atggcgcaag cgtttacgct
    16441 tgtgccgttc actgacatat atcaatatct aataaaacag gtaagttgtt agcataattt
    16501 cttgcactgc tatatttcca ttcaaatgcc tcgcatacaa aaccgcttac gatagggttg
    16561 ttgtgaatat agtttagctt ttcatcaaaa aatttctgag aatagattgt tatcggttgg
    16621 ttatgatgtt gccaaagttg gtattgcttg acattatttc gttgtttagc tgcccgagta
    16681 aacatccata ataaccattc ttttctactt tcttggggat ttgttttaat ttgctgaatc
    16741 aacattttag atgtaaatgt tttaaaatct cttattaaat ctgacgggtt ttggtctttt
    16801 gcttgaaata ataaatgaat atggcttggc ataatgcagt agccatataa aaaatcatcc
    16861 ctttttgttt tcgacaataa ttcaatgatt ggataatggt ttgaaaataa tcttctcgaa
    16921 taaatagatc aatccaataa actgttgcaa aactaacaaa ataggctccg ctcttctcat
    16981 aaaacttata ttttctactc attatgaacc tctaaagtgc agtttatgct gatagtaata
    17041 tcggtgaaca gttagggtta gcaattcatt ttattttttc gatgtagatc gcaaaaatag
    17101 aattatgctt ggcacaagcg aggacgctta cgccatcttt actccaaaaa tgtgagatag
    17161 atcacatttt caaatatgcc caaaaaaatt taatgtgatc tacttcacat ttatgagaga
    17221 aacatttttg taacttttga aagtggtgct aatttaagca cgattttttc aaccctaatt
    17281 ttctctcctt ttttgaggtt tctatgtcta atttaaaagt gaaagtccaa ggttttgggc
    17341 gtttcttatc gaatatggtg atgccgaata tcggtgcctt tattgcgtgg ggctttatca
    17401 ccgccctgtt tattccaacc ggttggttgc cgaacgagac ttttgctaaa ctggtcggtc
    17461 cgatgattac ttacttgctg ccactgttaa tcggttacag cggtggtaaa ttagtcggtg
    17521 gtgagcgtgg tgcggttgta ggtgcgatta ctacaatggg tattatcgtg ggttctgaaa
    17581 tcccgatgtt cttaggtgcg atgattgtag gtccattagg cggctgggcg atcaaaacct
    17641 tcgataaagc gattgacggc aaagtaaaaa gcggttttga gatgttggta aacaacttct
    17701 cggtcgggat catcggtatg attttggcaa tgctttcatt tgctttcatt ggaggtatcg
    17761 tcacccaaat ctctgcggca ttaggtgcag gtgtgggggc gttggttgaa gctggtcttc
    17821 tgccagtaac ctctatcatc gttgaaccgg cgaaaatcct attcttaaac aatgcgatca
    17881 atcacggtgt gttcacgcca ttgggtacag cacaagcaca agaagtggga aaatctattc
    17941 tgttcttgat tgaagcgaac ccgggtccgg gctttggtat cttgcttgcc tatatgttct
    18001 ttggtcgtgg cacagcaaaa caaaccgctg gtagtgcgtc aatcattcac ttcttaggcg
    18061 gtattcacga aatttatttc ccgtatgtgt taatgagccc acgcttaatt cttgcagtga
    18121 ttgcaggcgg tgcaaccggc gttttaacca actcattatt aggtggcggt ttaatcgcac
    18181 cggcttcccc gggttctatt tttgcgattt tagcaatgac cgcaccgggt gctcactttg
    18241 cagttgttgc ctccgtgatt ctttcttgtg ccgtgacttt tgcgattgcg tcaatcttac
    18301 tcaaaaccca aaaaggtgaa gcctcattgg aagatgcaca agcacaaact caagcgatga
    18361 aagcagaaag caaaggcgta gtaaatggta acatcaacaa aatctatgta gcgtgtgatg
    18421 ccggtatggg ttcaagtgcg atgggtgcga gtatgttacg caaaaaagtg caagcggcag
    18481 gtttaaacat tgaagtacaa aacttagcga tcaacgactt accgcaagac gcacaactgg
    18541 tgattaccca caaagactta accgctcgtg caacacaacg tgtggcaacg gctcaacact
    18601 tatcgctcac taacttctta gacagtgcgt tctatgacag cttagtggca aaactggcta
    18661 accaccagcc acaacaagcg gtgcaaaata cacaaaattt tgcaaacccg gtggaagaac
    18721 ctcaagccga agggttaaaa ttgcaagtag cccaagtctt tttaggctta caagctaaca
    18781 gtaaagaaga agcgatccgc tttgccggtg agcagttagt taaagcgggc tttgttcaac
    18841 caagctatgt agatgcaatg ttcgcccgtg agcaaatggt ttcaacttac ttaggcgaag
    18901 gcttagctgt gccacacggt acatctgatg ccaaagacag cgtactgaaa accggtatcg
    18961 tggtctgcca atacccgaac ggcgttcgct tcaccgatga agaagacggc gtggcgaaac
    19021 tggtggtcgg tattgcagca aaaggcaacg accatatcca aattttaagt gcaattacca
    19081 acgcactgga cgatgaacaa gcaatgaaaa cactgacctc aacaaatgac gtggaaaaag
    19141 tgttagcatt attatctaaa taatcaccct gttgtgtagg ggcgggtcct gtgcccgccc
    19201 attatcctac aaaatttagg gcgggcacag gacccgcccc tacgttttat agaggaacaa
    19261 tcatcaatga acgcactcca ttttggtgcc ggtaatatcg ggcgtggctt tatcggcaaa
    19321 ttgcttgctg atgctcgcat tttcgtgact tttgccgata ttaaccaaac tcaaatcgac
    19381 caaatcaacc aaaacaagca atacggcgtg aaaatcgtgg gcgatgacag ccgtgtggaa
    19441 attgtcaaaa acattgcggc aattaactcg aaagacgaaa atgccgtgat tgagcaagtg
    19501 aaaaacaccg atttaattac caccgcagtc ggcccgaatg tgcttggctt tattgcccca
    19561 ctgttcgcca aagcgttagt ggctcgtgtg gaaagtggca acacccaacc gctgaacatt
    19621 atcgcctgtg aaaatatggt gcgtggcaca actttcttta aaggtaaaat ttttgagcat
    19681 ttaactgccg agcagcaagc agaaattgaa aaagtggttg gttttgttga tagtgcggtg
    19741 gacagaattg taccacctgc cgagccgaac cctgctgatc cgttggaagt cacggtggaa
    19801 gagttcagcg agtggattgt ggatcaaacc cagttcaaag gtgacattcc aaacatcaaa
    19861 ggaatggaat taaccgataa cctgatggcg tttgtggaac gcaaactgtt caccctgaac
    19921 accgggcatt taatctgtgc ctacttgggc aaacaagcgg gcgtgaaatg gattaaagag
    19981 gcgattgcga ttgaggaaat caaaactcag gtgaaagcca caatggaaga gagcggtgct
    20041 gtgctgatta aacgttacgg cttcgaccca caagcccatt cagcgtatat cgaaaaaatc
    20101 ctcaaacgtt ttgccaaccc gtacttaaac gatgacgtaa accgtgtagg gcgtgagcca
    20161 atccgcaaac tgagcgaaaa cgaccgcttg atcaaaccgt tgcgtggcac gttggaatat
    20221 ggcttaccgt atcaaaactt ggtcaaaggc gtggtgatgg cattgcaata tcgcaatgag
    20281 gaagacccac aagcggtcga acttgccgaa tttattgcaa atcacggtgt ggcggcagcg
    20341 gtggaaaaat acaccggttt aaccgaccaa gcggtcattg ctcaagtggt ggaattatat
    20401 caataattct tgacactatg atggattaca tcgacaaact cagtgaggaa accaccctgc
    20461 gtggtttcct tagcattgcc aatcaacgct ttgccaaagc ggttgaacag ttgattgaac
    20521 gagtgtttcg caaaacggac tttgcgttaa aatccgtggt ggattccttg tttgaacatc
    20581 aaggaccgct ggctgacatt gaagtacgtt taaaagtgtt gcttgggttg ggggtgatct
    20641 cacctaaagt gtttgaagat atctcgcttt tccttgaagt caaacagcat ttgaatgagg
    20701 aagaagacga gttcgcattt tctcacccgg cggtagtgaa atttgccaaa gatttgcacc
    20761 atattgattt tttgcctatc aatgaattgc tgaatatgaa agcgagtgca gcgaataaag
    20821 attcgatgct ctttcaaatg cagcaaatgc ggttggaaaa aatggtacgt tcaagcctga
    20881 ttttggcggt aactgaaatt gacgaacaac tgaatgtgga aagcccattg taaaaatttc
    20941 gtcaaaacta accgcttgta ctgagcattc acgccgtacg tgattgcttt ttcctttctt
    21001 tttgatctta tcggcttttt tctttgctta aagcggtgta atctatccct aactattttg
    21061 gaggatatgg ttatgcaatc gttgctctct tttgtgccgg attttcttaa gcaagaactg
    21121 ttttgggtaa tggcgttatt ggtatttgcg atttggcaat ttatccaaaa caagttgcgt
    21181 atggacgtgg ttgccttaat tgtaatgctg ttttttagcc tgactaatat tttatcagtc
    21241 aaagaagtgt tggcaggctt aagtgaccca aacgtggtgc tgattgcgtt gctctttatt
    21301 gtcggagaag gtttggtgcg aaccggcgtg gcgaaccaag tcagcgattg gttgatgcgg
    21361 gtgtcgggag cgagcgaaac caaagtgctg gtgctgctaa tgctctgtat tgccgggctt
    21421 ggatctttta tgagttccac cggcattgtg gcgattttca ttccggtggt gcttgccatt
    21481 tgctcgggaa tgggaatttc tcctcgccgg ttaatgatgc cgttaagtgt tgccggactt
    21541 atcagcggta tgatgacctt aattgccaca ccacccaacc ttgttgctca ttccgaatta
    21601 gtaaaatcag gttttgaagg cttcaatttc ttcagtttta ctccgattgg attaattatt
    21661 ttagtgatgg gcatcggcta tatgctgatt gcccgccgtt ggttggataa tggtgagcaa
    21721 agcaatactc acagtcaggc tcaaaaattt tcaatgtcgc agttaattga agagtatcaa
    21781 cttaaagagc gagccaaaat ggtgctggtg gaagcagatt cagactgtgt cggcaaatct
    21841 atcgggcaac tgaatttacg gattgagcaa gggctaaaca tcattgcaat tcaacgcact
    21901 aagcggttta gaaaaattac cgtcagtgcc tcggtaaacg aaattttcca gccgaaagat
    21961 attttactgc ttgatactgc cctcgatgat gaaacttacc aacaacgctg tgaacagctc
    22021 aaacttcgcc cgattgagct aaaaggcgaa tacttttcct cacaatatcg ctcggtgggt
    22081 atggcggaaa tttcggttat gcctgaagca gaatgtatcg gtaaaactat tcgggaactc
    22141 aaattccgct caagatacca tttaagcatt gtcggcttaa aacgtggtga tgaaatcata
    22201 caagaggaac tgctcgatac gccactcaaa ttcggcgata ttttattggt gatgggcgta
    22261 tggcaacaaa ttcaccgtat ggacgacgat catcgagatt tcttcttgct tagagatccg
    22321 gcagaaagta aaaacgcccc tccggcgtta agccaagccc ctcacgctct gttctcagtg
    22381 ctgactatgg tgttgctgat gattaccggc attgtgccaa acgtgatggc agcgttgatt
    22441 gcctgcttaa tgttaggcaa gttccgctgt gtggatatga aaagtgctta tgattcgatt
    22501 catatgccaa gcattgtgtt gattgtgggg atgatgccgt tttctattgc tttacaaaaa
    22561 accggtggtg tggatttaac cgttaggctt ctacttgatg caatgcaagg cttagatgta
    22621 cattttgttc taatagtatt atttgtagta acagcgatta tcagtgcttt tatttccaac
    22681 acagcgactg caattttggt tatgccgata gccattgcga ttgctaacca acttggctac
    22741 tcaccggcac cttttgtgat gacggtcgct gtttcagcct ccgccgcttt tatgacgccg
    22801 gtttcttcac cggtaaatac gatggtatta gcaccggcag gctataaatt tattgatttc
    22861 gttaaagttg gtgtgccgtt tactgtactg gtgatgctgg tgtcggtgtt tgtgattccg
    22921 atgttatttc cgctctaaac actcctctaa attacaagcg gtcatttttg gtaatctgac
    22981 gttgacggta aaattgttga gtatgtctgg caacatagtt cccattttta gcccgattat
    23041 gccggatttc ccgttggata gtcgagattg accgcttgtt atccaattag gcttttttct
    23101 tgctgatggt atctacccaa actgcaagca gtagaattcc acctttaacg atatattgcc
    23161 agaaggttgg cacatcaagc atactcatac cgttatccag caaagcaata ataaatgctc
    23221 caataactac gccataaatg ctaccgatac cgccggctaa actggctcca ccaatcacgc
    23281 aggcggcaat cgcatctaat tcggcatttt gcccggcgga aggtgcgccg gctccaagac
    23341 gtgagcttaa aatcaagcct gcaatggcaa ctaataagcc gtttaaggca aaaatcgcaa
    23401 gtttgacttt ttcaactgaa atccccgaga ggcgagcggc atcaatgttg ccaccaatcg
    23461 cataaatgtg acgcccgaag gcggtttttc tggccaagaa tgtgccggca atggctagaa
    23521 tcaccaacac caataccggg aatggcacac cacggtaatc attgagtagg tagatcgcac
    23581 ctaatacgat aattgcaact agtccgtatt tcagcgtgtc tttcgacata gaaggcacag
    23641 taagattgag tttttgtctg gctttgcgtt ggtagctacc ccaaacaata aagcaaaata
    23701 tcccgatagt acctaaaatc atacccacca tatcaggcaa atagccttga ccaatggttg
    23761 tcatttcatt gctaatcggt gaaacggttg tcccgttggt taagccgacc aatacaccac
    23821 gaaacgccag cataccggca agagttacaa taaaggacgg cactttttga taagcaaccc
    23881 accaaccgct ccaagcaccc agcatgacgc ctaaaattaa agtaactata atagttaacg
    23941 agagcggcca gccccaccat acattactga tggcggcaaa tccaccgagt aagcccatta
    24001 gcgagccaac cgataaatca atttctgcgg aaataatcac aaataccata ccgatggcaa
    24061 gaataccggt aatggaggtt tggcgtaaca ggttggagat atttctggca cttaaataag
    24121 caccgtcagt tgcaatagag aaaaagctca taatcacaac aattgcaatc agcataatat
    24181 aaacttgtaa attaatcgat tttaacttat tcataggtta ctccttgagt gccgcttcca
    24241 ttactttttc ttgggtaagg tcttggttaa tcaaatctgc tttaattttg ccttggtgca
    24301 taaccaatac acgatcacta atgcctaaaa cttcaggtaa ttcggaggaa atgacgataa
    24361 ttgccatacc ttctttagct aattggttga tgagcttata aatttcatat tttgctccca
    24421 cgtcgatacc acgggtcggc tcatctaaaa tcaggatttt agggttaagc aataggcatt
    24481 ttgccaaaat tgctttttgc tgattaccgc cacttaaccg tccaattgct aattcaggcg
    24541 aggaagtttt cactttcagt tttgcaatag attggttgat aatggtttct tccagcggtt
    24601 catttattac ttttttgcca aaacaaaatt gcggtaatga agagagagtt atgtttttac
    24661 ccacccccat aattggaatg atgccgtgtt ttttgcgatc ttccggcacc atcacaatat
    24721 ggttttcaat cgcttgtgca caatttttga ttttaatcgg ttgcttatgc agaaaaatat
    24781 caccttgata tttgccttgg taagagccaa aaatacattg tgccatctcg gttcgccctg
    24841 aaccgaccaa accagcaata cctaaaattt caccttggcg tagtacaaaa ttagcattat
    24901 caacccgttt gatatgtgta ttggttgggt gccaagcggt taaattttca actcgaagga
    24961 cttcttcacc aatattgtgt ggctcgtgtg ggtagagcga ggtaatctca cgtccaacca
    25021 tcatcgtaat aatatcgtct tcactcattc cctctgcact gcgagtaccg atatgttcgc
    25081 cgtctcgaat gacacagatt ttgtcggaaa tggctttcac ttcattcaat ttgtgtgaaa
    25141 tataaacaca agcaatattg tgagcttgta aatctttaat taaactcagc agaatctcgg
    25201 tttctttttc ggttaacgag gcggtaggct cgtctaaaat caacaaccgt acctgtttat
    25261 tcaatgcttt ggcaatttca accaactgtt gttgccctaa acctagctct ccaactttgg
    25321 tgtttgggtc aatatccaac tgcacttgtt caagtaaggt tttacaacgc agatacattt
    25381 cattgtcatc tgtaatccct gatttggtaa tttcattccc taaaaacata ttctccaaaa
    25441 tcgacatatt ttttactaag gtcaattctt ggtgaataat cgaaatgcct ttttcttcag
    25501 tatctttgat atttctggca atcaattttt cgccggaaaa atagatttcc ccttcataat
    25561 caccggcagg ataaattcca cataatactt tcatcagagt tgatttgcct gaaccatttt
    25621 caccacataa agaaagtatt tcgccggttt caagggaaat tgaaatatta tttaatgcca
    25681 caacctcacc gaatttttta gtgatgttct tcatttctaa taatttcgcc atacttagtt
    25741 agccccttat tttcaaaaat ggcgaggaag cctcgccatc tagaaaaatt aaagtgattt
    25801 atttatatac tgcatctttg gtatggaagc cgtctttaat tactgtgtca tcaatatttt
    25861 ctttagttac aacaaccggc tctaataaat atgccggtac ttctttcata ccgttattta
    25921 attgagcatt catttctact ttctcatctt tacctaatgc aacagcaatt tcggcagctt
    25981 tgtcggctaa attggtaatt ggtttataca ctgtcatcgt ttgcgtaccg gcaataatac
    26041 gtttaattgc ggctaaatct gcatcttgtc cggaaattgc aaccttgccg gataaccctt
    26101 gggcacttaa cgcttgaatt gcaccgcctg cggttgcatc gtttgaagct acaactgcat
    26161 caatattatt tttattagca gttagtgcat tttccataat ctgtaatgct ttttcagcaa
    26221 gccaagaatc tacccattga tcgcctacca ctttaatttt tccactgtca attaacggtt
    26281 gtagcacctt catttgacct ttacggaaca gcttcgcgtt attatctaca ggtgaaccac
    26341 ccattaggaa ataattcccc tccggctttt gcccaataat gcttttagcc tgcaattcac
    26401 ctactttttc attatcaaat gaaacataaa aatccacatc ggcattattg attaagcggt
    26461 cgtaagcaag taccttaaca ccttctttct tcgcctcggc aatcacattg gataacactt
    26521 cgccgttaaa cggaataatc accaatacat caatatcttt attcaacata ttctcaattt
    26581 gtgagatttg tgcggttgca tcgccatttg ccgattgaac gaagactttt gctcctaagc
    26641 tttccgcttt attcacaaaa atatctcggt ctttttgcca acgctctaag cgtaaatcat
    26701 caattgacat accgattttt agatctttag caacagaagc attgctgagt accattaaag
    26761 tcgccgtagc aacggctaat aatttagact taagtttcat agttcacctc attggttatg
    26821 aaaatgtgag catctttttc gcccgtaagc agtctgggct tatttcaaat gaagttcaat
    26881 tatcatattt attaatatca ttatgatttt tcacttttgt gatctacgcc acattaacca
    26941 ataatcgaaa tccaaaaacg aaatatcgta attgaaggtt ttgcataatt ttccgatgat
    27001 gttaccgaaa gattattttt ccaattcata ggagcattat tatgtctaac tatttcgaca
    27061 aaatctcaaa agtacaatat gaaggggcta attccaccaa cccgtttgca ttcaaacatt
    27121 acaacccgga tgaagtggtt ttaggcaaaa cgatggcaga acatctgcgg ttagcggttt
    27181 gctattggca caccttctgc tggacgggta acgatatgtt tggcgtgggt tcgcttgaca
    27241 gaagctggca aaaaacgggg gatttactgc aaggtgcgaa gcaaaaagcg gaaattgcct
    27301 ttgaattttt ccaaaaatta ggcattcctt actactgctt ccacgatgtg gatgttgcac
    27361 cggaaggcaa caatttcaaa gaatatcagc acaatttcaa cactattgtg gatattttag
    27421 agcaaaaaca agctgaaaca ggcgtgaaat tattatgggg tacggcgaac tgctttacca
    27481 atcctcgcta tatgtcgggt gcttcaacca atccgaatcc ggaagtcttt gcttgggcgg
    27541 cagcacaagt ctttaccgca atgaacgcaa ccaaacgctt aggcggcgaa aattatgtat
    27601 tatggggcgg acgtgaaggt tatgaaaccc tactcaacac tgacttaaaa cgtgagcgtg
    27661 aacaaatcgg tcgctttatg cagttagtgg tggaacataa acacaaaatc ggttttaacg
    27721 gcactttatt aattgagcca aaaccacaag aaccaaccaa acatcaatat gactacgatg
    27781 ttgccaccgt ttacggtttc ttaaaacagt ttggtctaga aaaagagatc aaagtcaaca
    27841 ttgaagcaaa ccacgccact cttgcagggc atactttcca acacgaaatt gcgactgcca
    27901 ccgcattaga tatttttggc tcgattgatg ccaaccgagg tgatccgcaa ttaggttggg
    27961 ataccgacca attcccaaac agcgtggaag aaaacaccct tgtgatgtat gaaatcttaa
    28021 aagcaggtgg tttcacaacg ggcggtttca actttgatgc gaaaatccgc cgccaaagta
    28081 ccgatccgta tgatttattc cacgcacata tcggggcaat tgatgtgtta gccctttcgc
    28141 taaaacgtgc ggcaaaaatg ctggaagatc aagcattaca gaaagtagta gataaccgct
    28201 acgctggctg gaaccaagat ttgggcttac aaattctaaa cggtaaagca agccttgaag
    28261 atttggcaaa aatagtggaa acccaagggt tagatccaaa acctgtttcc ggtcagcaag
    28321 aatatttgga aaatttggta aatagctata tttaccgtta atctacaagc ggttgttttt
    28381 tatcaatttt ttgcaaagtt aagctcgatc tgatccccct ctttgctaaa gaggggtgat
    28441 agatctccaa aacttaaaat tgcaaatgtt ccgaataaaa tgaccgcttg tattcaataa
    28501 caaaggaata tttatgtaca ttggaatcga tctcggcaca tcgggtgtga aagtcgtgtt
    28561 actcgatgaa aatcaacaca ttattgccat cacacaaaaa tcacttccta tttttcgtcc
    28621 acacccattg tggtctgaac aaaatccgca agattggtgg aatgccacca atgaagcggt
    28681 gttagaactt tccgctactc aagatttaca agcagtaaaa gcgattagtt taaccgggca
    28741 aatgcacgga gcgactttac tagataacgc agataatgta ctttcccctg cgattttatg
    28801 gaatgacgga cgtagtttta tggaatgcga agagttagaa cagcttgtgc caaatagccg
    28861 tgaaattaca ggcaacctga tgatgccggg ctttaccgct ccgaaattgc gttgggttga
    28921 taaacaccag cccgatattg ccgaaaaagt cagcaaagtc ttgctaccga aagattatct
    28981 ccgcttgttg atgagcggcg aatatgcctc tgatatgtct gatgcctccg gcacaatgtg
    29041 gcttgatgtg ggtaaacgga attggaacaa atcgctacta aatgcttgcg gattagacat
    29101 tgaaaatatg ccgaaactgt ttgaaggcaa tcaaatcaca ggttatttac gtccaaccct
    29161 tgccgagcaa tggaaaatga aatctgtgcc gattgtggca ggcggtggcg ataatgctgc
    29221 cggtgcaatc ggcattggct tataccaaac cgggcaagct atgctctcat tggggacttc
    29281 aggggtttat tttgtagtga gcgataaatt tcaggctaat ccgcaaaaag cagtgcatag
    29341 tttttgccac gctttacctg atcgttggca tctgatgtca gtaatattaa gtgccgcttc
    29401 tgcggtagat tgggcagtaa aatcattggg actaaaagat gtatcgactt tattccaaca
    29461 ggttgaagca agccaaacgg catctgatgc tattttctta ccttatttat cgggtgaacg
    29521 tacaccgcat aatgaccctt atgcgaaagg cgtattttgg ggattaagcc ataatgacaa
    29581 ccaaataacg atggcaaaag ccgtgattga aggggtaagt ttcgctttgg ctgaagggat
    29641 tgaggtactc cacgaaactg gtgtagttgc tgacaatatt gcattaattg gcggtggagc
    29701 aaaaagtgcg tactggcgac aactacttgc cgatattagc ggtaaaacct ttgaataccg
    29761 caccagtggt gatgtcggtc cggcattagg ggcggcaaaa ctcgctcaaa ttgcgctaaa
    29821 cccaaatcag gatattgcta cgttctgtca gccgttaccc ctggaacagg tttaccaacc
    29881 aacccctgcc cgctatgcag aatatcaaca aaaacgccaa aaatttactg agctttaccg
    29941 gcggttaaag gggttataat tcttgccaac tattacaagc ggttgatttt gcagattttt
    30001 ttgtaaaatc aaccgcttgt atcacacttt agaaaacaaa ttaatcacca atacaccggc
    30061 aataatgagg ctgatgccga ttaagcctgc caagtcaatt ttctgtccga aagcaaaata
    30121 agcaacaagg gcggtaagga caatccctac gccggaccaa atagcataca cgattccgac
    30181 cggtagggtg cggaaaataa tagagagaaa gtaaaaggat aaaccgtata aggcgagcga
    30241 gccaatagtc ggtatagctt tggtgaagcc gttgcttaat ttgagtaaat tggtggcagc
    30301 gatttcgagg cagatggaaa tcgcaagtaa aatccaaacg ttcattgatg acctctttgc
    30361 gtaaatgcgt gctattttac ctgagttgca aaaaaattta aaaaaatcac cgcttgtttt
    30421 ttcctgatta agctatggta caatctttgg tgaatcagga ggtgtgaaat ggcacaggtt
    30481 tatatcgtac acggttatac ggcgaatgcc gataagcatt ggtttccttg gctggagcaa
    30541 gaattagcga agtgtggtgt cagttgtgag cggttgaata tgccggattc ggcaaagccg
    30601 tcattagatg gttggttaaa tcatcttgca caaaatgtgg agctgaccga tcaaacggtt
    30661 tttgtcgggc atagtttagg ttgcattgcc attttgaatt ttttagccaa aaattacgcc
    30721 aagattaaag gggcggtgtt tgtgtcgggg ttctatcaac cggtagaaaa cttacccgaa
    30781 ctgtctgcct ttaccaacta ttatgcgatt ttgccgagcg tatcgagctt tccaagctat
    30841 gtggtaagtg cgttagatga taaggtcgtg aatcaccaat acagtgatgc gttagcccaa
    30901 catttgcacg cggattatat tcggttaaat caaggcgggc attttttgga tagagaaggt
    30961 gttaccgaat tacctgtggt gttggagttg gtgaagaagt tactcgagtg aaacctgttt
    31021 tggtacaagc gaggacgctt gcaccatcta taagttgaat ttaaacaaaa gttatacatc
    31081 agtctgatag tgccagcgtc cacgctggta cttttgtata aaccaaccct attttgcaaa
    31141 aaaacggtaa aaataaaccg cttgcctccc tatgggaaaa cttacagttt aataaatcca
    31201 tcataaatgt gggtggcatc gccggtcata tacagaggat gccctactcc ttgccattca
    31261 ataatcagcg ttccaccggg taaatccacc tgcactttgt tatctaacaa gccttgcata
    31321 atgccgaccg ccactgtgcc gcacgcaccg ctaccacagg cttgggtttc gcctgctcca
    31381 cgctcataca cccgtaattt aacgtgattg cgattcacta cttgcataaa gctgatgttc
    31441 gcccgttcag gaaaacgctc gtggctttct agtaacggcc cgagttcatt taccggggcg
    31501 gttttcacat catcaacttg cagtacgcag tgcggattcc ccattgaaac cacgccacat
    31561 aacacggttt gtagctcggt gcgtaaaata tagtttttct caaatttatt tgctgtaaaa
    31621 ggcacttgcg aaggttccca aatcggttcg cccatattca cacgcacttt gccgtcttct
    31681 tgcaaactta gcaccatttt gccttttgcg gtgctaacgt gaatgtcttt tttgttggtt
    31741 aagcctttga gcgtaacaaa acgggcaaaa catctcgccc cattgccaca ttgcgacact
    31801 tcactgccgt ctgcattaaa aatgcgatag tgaaaatcta attcagggtc gtaaggcggt
    31861 tcaaccaata aaagttggtc gaaaccgata ccacgatggc gatcagaaag cgtacggatc
    31921 atctcttcgg taaggtaaac gttttgggtt acaccatcaa ccaccataaa atcattgcct
    31981 aagccgtgca tttttgaaaa ttgcatatta ttccttaatt aattagaaat tttattgatt
    32041 tagattatag agattagttt tatttattga tatagtacgt gccgattaat ttgatgaaaa
    32101 atgcagattt tttcaaacac agagtttcat ttacttattt aggagcaaaa aattatgtct
    32161 gcgatgttta ttatccaatt tgccatagtg ttactatgca ttttggttgg cgcccgtgta
    32221 ggcggtatcg gattaggggt gttcggtggc ttgggcttag ccattctttc atttgggttt
    32281 gggctaaaac ctgccggttt accgattgat gtaatgttta tgattatggc ggttgtgtcg
    32341 gctgcagcag ccatgcaagc agccggtggt ttggactata tgatcaaaat tgccactcgc
    32401 attttgcgta aaaacccgaa atacattacc tttatcgcac cgcttgtaac ttggacattc
    32461 accgtgcttg ccggtacagg tcacgttgct tattctgtat tacctgtaat tgcagaggta
    32521 agccgccaca acggtatccg ccctgagcgt ccactttcaa tggcagtaat cgcctcacaa
    32581 tttgcgattg tcgccagccc gattgctgcg gcagtagttg ctgtggttgc cttccttgaa
    32641 cctcaaggtg ttacattggg taacgtgtta agtgtgacta ttcctgcgac cttattgggc
    32701 ttaggtttag cttgtgtgtt tgtaaataaa atgggcaaag aattaaaaga cgacccaaat
    32761 tatcaacgct tattgcaaga ccctgaatat gtgaaagaaa atcacattac taccaatcca
    32821 accgaattac cgttaaaacc gactgcgaaa ttatcagtag gcttattcct attcggtgca
    32881 ttattagtgg tattaatggg cgcaattcca gctcttcgcc ctgtgtttga tggtaagccg
    32941 atggggatgg ctcacaccat tgaaatcgtg atgttaagta ttggtgcgtt gattattctt
    33001 acctgtaaac cggacggcaa tgaaatcact aaaggttctg tcttccacgc cggtatgcgt
    33061 gcagtgattg cgattttcgg tattgcgtgg ttaggcgata ccttaatgca aggtcatatg
    33121 gatgaagtga aatcaatggt gtcaggctta gttgaaaccg caccttgggc atttgctttc
    33181 gcattattcg tactttccgt attggtaaac agccaagggg caacggttgc aaccttattc
    33241 cctgtagcga ttgctatcgg cattcctgca ccggtgctaa tcggggtatt tgtggcggta
    33301 aacggctact tcttcattcc gaactacggc ccgattattg cctcgattga cttcgacacc
    33361 accggcacaa cccgtatcgg taaatatatt ttcaaccata gctttatgtt accgggctta
    33421 cttagtatga ttttcagtat cggatttggt ttattacttt ccaatatcct actttaatcg
    33481 ctcgcaaaac ctcccgtttg ggaggttttt tatttgcaaa attttcagca aaaacgaccg
    33541 cttgtaacgg ctcttgcaag cattacacct atcaaagtaa gcaaaagccc accgattaaa
    33601 tgtaaactca gcactgtcac aaactgcccc catttttctg ctaatagttt ttccgttagt
    33661 tctaaagaga aactggaaaa ggtggtaaaa ctgcctaaaa aaccgctgat gaacagcaac
    33721 cgctggctct cgcctaaatt aaaccccatt gcaataccaa ttaacaaaca accaagccaa
    33781 tttgctgcta aagtaccaaa ggcgaattgg ctgagtatcg ggtttagcca aacggctaat
    33841 tgccagcggc acatagctcc aagtaccgcc ccgattgaaa tcataagtat tgaagacatc
    33901 atttttcctc atctaacgtt ttctcacatc atacctaaaa atgagtgatt tagaaaaata
    33961 atgtgatgga tttcacaaat ttgaaacttc ctatttgctt aaaaaatgcc gctttggcaa
    34021 tatcactccg aaatatgttc atattcaaac attttttcat cactttccaa tcattttcat
    34081 cttaaggaga aactttatga gcaaatcctt acgtaatgcc tgtattggcg aatttatcgg
    34141 tacaggtttt attcttttct ttggtgctgg ttgtgttgct gctgctcagg tagccggtgc
    34201 gaatttcggt ttatgggaaa tttccatcgt ttgggggctt ggtgtttcca tggcaatcta
    34261 catttctgcc ggtatttccg gtgctcacct taaccctgcg gtcactatag cacttgccgc
    34321 attctacggc tttgaaaagc ataaaatttt gccctatgtc atcgcccaag ttgccggagc
    34381 gttttgctct gtcgcactga tttattttat gtatagcgat ctctttactg ccgccgaagc
    34441 cgctcaaggc attactcggg gcgaaactgt tggctttgcc ggtgtgttct ccacctatcc
    34501 aaaccctaat attacattgg ttaccgcctt catcgttgaa tttgtgatta ctgtggtgtt
    34561 gatgtccact attttagcga ttggcgatga taaaaacggc ttgccgaata aagccttagc
    34621 cgcattactc atcggtttat tgattgcggt aattggtgga gcaaccggcc cgttaaccgg
    34681 ctttgcgatg aacccggctc gtgattttgg tccgaaactc tttgcttatc ttgccggttg
    34741 gggtgaaatt gctttaacag gcggaaaaga aattccgtat ttcatcattc cgattgtcgc
    34801 cccaatctgc ggtgcattat tcggtgcttg gggttataaa aacctcattc ataacaatct
    34861 tccagcaaat actcaggagt aatctatgga aaaacaatat atcatcgctc tcgaccaagg
    34921 tacaaccagc tctcgtgcgg ttttattaga taaaaatgcc aatatggttg aagtggctca
    34981 gcgtgaattt acccagattt atccgcaagc aggctgggta gaacataacc cgatggaaat
    35041 ttggtcaagc caaagttcca ccttaaatga agtggtggca aaagccggta ttaagccgga
    35101 tagcattgcc gcaatcggga tcacaaacca acgtgaaacc acgattgtgt gggaaaaaga
    35161 gaccggtaaa cctgtttata atgcgattgt gtggcaatgt cgtcgcacat cggatattac
    35221 cgataaatta aaagcagacg gacacgagga atatattcgt aaaacgacag gcttggtagt
    35281 cgatccgtac ttctccggca ctaaagtgaa gtggatttta gataacgtag aaggggcaag
    35341 agaaaaagca gaacgaggcg aattgctgtt cggtacagtt gatacttggt tagtgtggaa
    35401 attaacccaa gggcgggtac acgttaccga ttacaccaac gcttctcgta ctatgttgtt
    35461 taacattcac accaaacaat gggacgataa aatgctggaa ttgctcaata ttccacgctc
    35521 aatgttacct gaagtgaaaa attcatcaga agtttacggg caaactaata tcggcggtca
    35581 aggcggtgtg cgtattccgg tcgcgggtat tgcaggtgac caacaagccg ctctctacgg
    35641 tcatttatgc gtgaaagaag gacaagcgaa gaatacctat ggtacaggct gttttatgtt
    35701 gatgcacacc ggtaacactg cgattcaatc tgaaaacggt ttattaacga ccattgcgtg
    35761 taacgcaaaa ggcgagccgg aatatgcgtt ggaaggttca gtatttattg ccggtgcgtc
    35821 tattcaatgg ctgcgtgatg agctgaaaat tgttcacgat agctttgaca gcgaatattt
    35881 tgccactaaa gaagatgact ctaacggtgt ttatgttgta cctgccttta ccggcttagg
    35941 tgctccatat tgggatccgt atgcacgagg ctcaattttc ggtttaaccc gtggggcaaa
    36001 ccgtaaccac attgtgcgtg cgaccctaga gtctatcgct taccaaaccc gagatgtgtt
    36061 agaagcaatg cagtctgact ccaaagcgaa actacaagcg ttacgtgtgg acggtggtgc
    36121 aaccgcaaac aacttcttaa tgcagttcca agccgatatt ttagacacca aagttgagcg
    36181 tccgaaagtg aaagaagtca ctgctttagg ggcagcttat cttgcgggta ttgctgtcgg
    36241 cttctggaaa gatttggaag aactgaaaga caaagcggaa attgaacgta ccttcacacc
    36301 ggacggcgac caagaaaaac gggcgaaacg ctataaaggt tggaaaaaag ccgttcgccg
    36361 ctcattagaa tgggcaaaag aagacgcaga agagtaaaaa cgcaacctgt gcataaacaa
    36421 agggagtata ggcaactata ctccctttaa gatcttttag ttatattaaa tagtgatttt
    36481 gatttgttct aaattagcct tttctttatg atgagccaca ccaataaaca acctgataag
    36541 gaacatttat gtctcaattt caaattcaca ctattgaatc cgctcctgaa acggcacaag
    36601 aagccctaaa agcagtgcaa caagcaaacg gttttatccc aaatctgatc ggcgtattag
    36661 ccaacgcccc aacagcactg gaaacttacc gcactgtggg tggtattaat agtcgtaaca
    36721 gcttaaccgc tgaagaacgt gaagtggtac aaatcaccgc agcagtggtc aacggctgtg
    36781 gtttctgcgt ggcaggtcat actaaaatcg ccttaaaagc attaaaaatg cctgaagaag
    36841 tggtaaatgc tttacgtgcc acggctcgca tagacgataa caaattagac actctggctc
    36901 gcttcaccct tgcagtaatg ttacaaaaag ccaaattaac cgaagcccaa ttaaacgaat
    36961 tttttgccgc aggttacaac caacaaaatg cgattgatgt gattttaggc gtgagcctcg
    37021 caaccttatg taactatgtg aacaacattg ccgaaacgcc aatcaaccct gaattgcagc
    37081 cttttgcgta atcgaaaata ataaaattta gggcgcttaa tgcgtccttt tttatattta
    37141 caagcggtta tttttattca attttttgca aataaaaaga ggcaatattt gcctctcagg
    37201 tttaactatt tgccaatacc attctcagct gcaccgcact tggtgtaacc acaggtaaga
    37261 atgcggcttc tttccaacgg cggctgaagc ctgaagtatc gtttttcaaa taaccacgtc
    37321 caccgctcgc ttgtagttct aacagaatgg tttgggcgac gacattaaca atatcgattc
    37381 tggcttggaa cagctctttc gggttacgaa caaaatagcc ctcttcattt aagccattga
    37441 ataaacggtt tttgatgtcg tttaacgcat caacttggga ttgccattca gacgttaaaa
    37501 ttgcacggaa ggagagcgat ttttcaattt ccgataaact tttttctacc aagccaaagg
    37561 gtaagccaaa ctgtaagcct aagaacgcag ggcgggttgt ggctaaaaat tcgggggcat
    37621 tatcggacaa aatccactcc tcttttaatt ccacattgtc aaacactaaa gcagtagtat
    37681 tcgtgccttg tagcgacata aattccagct ctttagtgcg ggttaagcct tcggcagttg
    37741 agggaacggc aatcaccagc ggtttgcgtt catcttggaa gccggcaaca aaactggcaa
    37801 caaagcggta tgaacgtaaa ttggttaccc aaggcaggcg gccatttaaa tagcgtttac
    37861 cgttttcttc acgaatattc acattcaact cttcaatgcc ggagagaaat ttcattgcat
    37921 tcgataaacc tgttcctgcg gttaaattgc catttagcag atccgccaaa tagctttggc
    37981 ggggaatagg attttttgag gcgataatat gctcgataaa ggtacgatgt ccccacgaaa
    38041 caaatgccgc agccagcgag tgattaccca gttcggtaac ggctttgatc gcatcaacca
    38101 aagtgccacc gctgccgcca agcgtttccg gcacgccgat tttatatagc cctagtgcgg
    38161 caatttgtgg aatgagtaaa tccgctcgtt cgttagattg atctaaattt tcggcgttct
    38221 gctgaagcca ttgaataaaa gaagatgaaa gaaccgacat tgtgtttacc tcgtataatt
    38281 agtttacttt gtcgctatct taaatcgctt ttttatgccc tagaaataaa ataaattaag
    38341 gtaatatgct tttttggaat aaacaagcgg tcgatttttt gcaaaaaaat gcaatttttc
    38401 gaccgcttgt caatttatct gccgcctttc gctaaagcca agcggattaa gctatccaac
    38461 gcccagccga ttaagccgat aattataatt accgccatca gttcggaata ggctaatcgg
    38521 tctcgggtgt ctaaaataaa atagccgagt ccttgattca cgcctagcat ttcgcacggt
    38581 accagcacca cccacgcaat cccaaccgcc acacgtaaac cggtgagaac gtgtccggta
    38641 atagccggta caacaatttt gcacagcagt tctttgcggg tagccgacat tactctaccc
    38701 agttgtaacc attgtggatc aatgcttttt acccctgagg tggtggaaat aatcaacgac
    38761 caaacggtgg caaacgccag taaaaaataa atcggcaaat cgcctacacc aagtaacatc
    38821 acgataatcg gcatccacga aagtggcgaa atcatacgta gtagttggaa tgacggcgtg
    38881 caaagcattt caaggtgctt ggagaagccg agcaataagc ccagcggtac accaatcact
    38941 aacgcagcga gtagcccgac agcaatgcgt tgcaaactcg cccataagtg cggaaaaatt
    39001 tcgccggagg aaatcagcca agtaaaactt tcccaggttt ttgccggagc taacattgat
    39061 gccatcggca accgttggct taacaaggct gtgccaagct gccaacctaa aagtagcagt
    39121 aataaaccgg ctaagccgta aatatatttc tgtttcatcg ttcgttccta tcgcttatac
    39181 actaatttgc tcggttcttg tccaaccggc ttgtaaatta aaggtttcaa cccaaccgcc
    39241 ttgctcaatc gcttttcgca caaaggtcgg ctcgttaatc gctttcgctg cctctaccgg
    39301 gtcaagctgt tgtaaaaacg cattattgcc ggcaatgtgg gtctgtttga gcattttgac
    39361 cagctcttcc atataggaat cgaacggata cggttggaaa ccgattcgct gttggtgcca
    39421 ctgagggttt tgaattgccc ctgtttcgat atatttatcc cactgagctt gggttggagc
    39481 cagtaccttt tccagcactt tttgatcgtg cggcgtgtag ccgctgcccc gtgccaacaa
    39541 ttttgctgtt tctgcccgat tttccagcgt ataaacctgt gcttcggtta aggcatttac
    39601 cactttttgc acccagtcgg ggcgatgctg aatatcatat tcgtgcatta gggttaaaca
    39661 gcaggcgtga tctttccaca catcgccact aaaacgcagc actttgccca cacctttggc
    39721 ttccgccaac gcattgaacg gctctgccac aataaagcct gaaattgcct cgcttgccaa
    39781 ggctgctacc atatctgacg gagccatcac acttaatcgc acttcgccgc tgttcggctc
    39841 tttttcggtg attttcaagc cattttctcg taataatcgt tgcaaaacga tattatgaat
    39901 tgaataccaa aacggaattg ctaccgtttt gccggagagt tcggcaatac tgttgatttc
    39961 agggcgaacc gttaaagccg aacctgccaa gtgattccac attaccgctt tcaccggagc
    40021 atttgctcca tatttcgccc acaaactcat aggcgaaagc agatgcacca cgttcacatt
    40081 gccacttaaa aaggcttcca ccaactgagc ccagctgcga aacatcaccg gtttttccac
    40141 ctgcacaccg tgcttttcaa ataagccttt ggcgtgagca actaacagcg gtgtggcgtc
    40201 ggtaatcggc aagtagccga taacaagcgg tcgattttcg gcattttttt gcacctgttc
    40261 gcaggcttga attaacggta aactacctgc aagggaaaac aggctgagca gtttcataaa
    40321 atctctacgt tgctgatcca tcactttctc cttaaatcac aaaatcaact gtctgctctt
    40381 gcaccgctcg ctgctgagcc tgtctaagcg tgtgtaaaat ctcgactcga atagggttca
    40441 gactcaataa atcagtacgg gggaatggag cagataaatg ccattttcca acaatatggg
    40501 cggtatctcc gccaagtaat aacacatcat ctgaaaccaa taaggcttca tcaatatcgt
    40561 gcgttaccat aatcgctgcc gcattatggt ggcggacaat atcgtgtaat aaggtttgca
    40621 tttggctgcg aatgacttca tcaagggcgg aaaagggttc atcgagcaaa atcactttcg
    40681 gctgacgagc caatgcccga gccaaattca cccgctgtgc cataccgccg gaaagttcat
    40741 gcggcataag atcgattgcc tcacgcaagc cgacttcctc caaagccgat tccaccctgt
    40801 gctgaatatc ggctttgctg agtggtaagc ggcattgaaa atctaaaccg aatgccacgt
    40861 tatttcgcac cgaaagccaa ggcaacaagg tcgccgattg aaacacaaag cccacatcgg
    40921 ggtgtggctg attgattttc tccccgaata acgccacctt tccgcttaag ggtttatcta
    40981 acccgctgat cacccgcagc agcgaggact tccccacccc actcggcccg agaatcgagg
    41041 ttaaagtgcc gctttctacg ttaaaagtga catcgtgcaa aatggtcttg gtttgctgat
    41101 ttttgcggta gccaagagaa agattcgtaa tgcttaggct cataaatccc cttttctagt
    41161 tcaatgttaa tgaaatgaaa atagaggatt cgtcttataa aagaaaagaa ttagtaattt
    41221 ttagttagaa caatttgtta taaaagtgta aaaaattggg cttttttgac cgcttgtgga
    41281 ctaaaaaata aagccataaa agctcaaaat ggcttttatg gctttgtttt ggttcgatta
    41341 gcgttccctt aaactttctg tgacagattc ttctaatggg ctgagtaaat aactcattac
    41401 actccgttca ccggttttga tttcagcagt tattgtcata cctgaactca aatcaatttt
    41461 tcgcccatca ggcgatgtta gattcttcct atctatagct atagttgcat taaaaactaa
    41521 gcctacatta ggttgttcaa tcgcatccgg gctaatatgt ttaattcgac cagttagata
    41581 accatagcgt gtataaggga aagtttccac tttaataatc acctcctgcc ctgctgcaac
    41641 aaagccgata tctttgtttg gaaccagagc ggtggcctct aacacatcgt cttccggcac
    41701 aatgatcatc aaggtttcag cagtcgtaac aacaccacct atagtgtgaa ttttcagttg
    41761 ctgaaccgta ccggaaaccg gtgctctgat catcgaggcc tgtctgcgtt gattattttt
    41821 ttctaactcg agccgaagtt ggcgttcatt ttcaatatgt tgctttaatt tttccaacac
    41881 atcgctttta aagaattgcg tgatcaattc aagttcttct tttacattga gtagatcatt
    41941 ttctaattca tttaattttg agcgataaac agctagctca ttctgagcct caattaattt
    42001 attttcttgc gcaagaagtt cgtgcttaga taaagacttc tgtttataaa gtgccttaaa
    42061 gtcttttaat ttttcttgtt caatacgtgt tgcaccttca tatttacgga cataggcaaa
    42121 tattgtttgt ttttcagcct ctttacgctt atacgctaaa gttttctgtg ttttttgttt
    42181 ttgccaagtg gtgtattgct cctcaattaa gtgtttaata cgtagtcgat cttcttccga
    42241 tgaatcttta aattcggttc tagataaatc aatcaccggc aaggactctt tttcaatggc
    42301 agtaagcaaa gtttggtagc gatagttctc cagtttagct aaagaaagtg aagccatggt
    42361 ctttttgata tctgcatcag aacccaatgc agttaagctg actaataatt gccctttttc
    42421 cacaaactgc ccatctttaa cgaaaatttc ttgtacaatg gcgttttcaa tcggtttaat
    42481 ttctttactt ctgccactaa aagttaattt accgggagca gtcgccacaa tttcaacttt
    42541 gcttacactg gcaagcacaa ttgccacaac taaaaatagc ataatcaaat aagcaattag
    42601 acgtggtttt ttagaaaccg gggtttcaat cagttctaaa tgtgccggta aaaattcact
    42661 ttcgtctttt tttctgtttg ggtggtctaa ttctttacga attttccata cttctgccca
    42721 aatgttttta tagcgtagga aaaattcata aataccacta agccatattt tcattgtttt
    42781 ccttcttaat taagttgtaa ttggtgtaag taggaataaa gtccgttact gttttgcagt
    42841 aattcgtggt gcttgccttg ctcaacaatt tccccctttt ccatcacaat aattcgatcc
    42901 gcatttttga cggtcgataa acgatgtgca atcaaaatca cggttctgcc ttggcatatt
    42961 ttttgcatat tttgcataat aatatgctca gattcgtaat cgagggcact ggttgcctca
    43021 tcaaaaatca ggattttcgg gttgtttacc aaagctcgag caatcgcaat ccgttggcgt
    43081 tgcccgcctg aaagccccgc tccttgttca cccacaatgg tgttataacc ttcacgcaat
    43141 tctgaaataa aatcgtgagc ccctgctaat tttgctgcat aaattactcg ctccattggc
    43201 attcctggat ctgatagcgc aatattttct cggatactgc ggtttaataa cacattatct
    43261 tgcagcacta cacctatttg acggcgtagc cagtttggat cagctaaggc tagatcatgt
    43321 ccatcaatca aaacctgccc attttcagga atataaaaac gttgcagtaa tttagtcaga
    43381 gtacttttgc ctgaaccgga acgtccaaca atcccaatca cttctccttg cctaatttct
    43441 aaattcacat tatttaaaat agttgg
//
LOCUS       DANKBV010000003        42829 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.003, whole genome shotgun sequence.
ACCESSION   DANKBV010000003 DANKBV010000000
VERSION     DANKBV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 42829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42829
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.003"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..338)
                     /locus_tag="PWY43_000110"
     CDS             complement(<1..338)
                     /locus_tag="PWY43_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2135034.1"
                     /translation="MAGAKEIRTKIASVRNTQKITKAMEMVAASKMRKTQERMAASRP
                     YAESIRKVISHIAKGNIEYKHPFLTPRPVKKVGYLVVSTDRGLCGGLNINLFKTVLHE
                     LKEKDDQGVKS"
     gene            complement(364..1905)
                     /gene="atpA"
                     /locus_tag="PWY43_000111"
     CDS             complement(364..1905)
                     /gene="atpA"
                     /locus_tag="PWY43_000111"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2135035.1"
                     /translation="MQLNSTEISELIKKRIAQFNVVSEAQSTGTIVSVSDGVIRIHGL
                     SDVMQGEMIALPGNRYAIALNLERDSVGAVVMGPYADLAEGMQVQCTGRILEVPVGRG
                     LLGRVVNTLGQPIDGKGEIQNDGFSPIEVIAPGVIDRQSVDQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTIANVVRKLEEHGAL
                     ANTIVVVASASESAALQYLAPYAGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERASRVNADYVERFTNGAVKGQTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESGLFNAGIRPAVNPGISVSRVGGSAQTKLVKKLAGG
                     IRTALAQYRELAAFAQFASDLDEATRKQLSHGQKVTELLKQKQFEPMSVAQLGLSLAA
                     AEYGYLDDIPVERVGSFEANLLAYAQSNYGEFMQELSKSGNFNDEIKDKLNEILSGFK
                     KNSAW"
     gene            complement(1918..2451)
                     /gene="atpH"
                     /locus_tag="PWY43_000112"
     CDS             complement(1918..2451)
                     /gene="atpH"
                     /locus_tag="PWY43_000112"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL2135036.1"
                     /translation="MSELSTVARPYAKAAFDFALEQGQLEQWQQMLTFSAAVANDEQV
                     ASFLNSSLASGQISETFIKICGEQLDQYGQNFIRVMADNKRLAVLPAVLNAFLELRAE
                     QESIKDVEVVSADKLTDAQEAKIANAMEKRLGTKVRITSSIDSSLIAGVIIRYDDVVI
                     DGSSRGQLNRLSQELSL"
     gene            complement(2465..2935)
                     /gene="atpF"
                     /locus_tag="PWY43_000113"
     CDS             complement(2465..2935)
                     /gene="atpF"
                     /locus_tag="PWY43_000113"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL2135037.1"
                     /translation="MNLNATLIGQLIAFALFVAFCMKFVWPPLIRAIEERQANIASAL
                     ASAEKAKQEQADSKAAADQEILRAKEEAQNIIDLATKRRNEILESVQAEAEVERQRII
                     EQGYAEVESERKRVQEELRQKVAALAIAGAEKIVGRSVDTAANNDIIDKLVAEL"
     gene            complement(2996..3250)
                     /gene="atpE"
                     /locus_tag="PWY43_000114"
     CDS             complement(2996..3250)
                     /gene="atpE"
                     /locus_tag="PWY43_000114"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDL2135038.1"
                     /translation="MESVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPEL
                     ASSLQTKMFIVAGLLDAIAMIAVGISLLFIFANPFIDLLK"
     gene            complement(3368..4156)
                     /gene="atpB"
                     /locus_tag="PWY43_000115"
     CDS             complement(3368..4156)
                     /gene="atpB"
                     /locus_tag="PWY43_000115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL2135039.1"
                     /translation="MAGTTAEYISHHLSFLSTGEGFWAVHLDTLFFSLVAGAIFLWVF
                     SRVAKNATTGVPGKLQCFVEIVIEWVNGLVKDNFHGPRNVIAPLALTIFCWVFIMNAI
                     DLIPVDFLPQLANLFGIHYLRAVPTADISATLGMSICVFALILFYTVKSKGFGGLVKE
                     YTLHPFNHWAFIPVNFILETVTLLAKPISLAFRLFGNMYAGELIFILIAVMYMADNFA
                     LQALGIPLHLVWAIFHILVITLQAFIFMMLTIVYLSIAYNKADH"
     gene            complement(4179..4562)
                     /locus_tag="PWY43_000116"
     CDS             complement(4179..4562)
                     /locus_tag="PWY43_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDL2135040.1"
                     /translation="MSAVINRAKSQYKQALKVEFFLLCVFALGVSLWKMNFNFSFLGG
                     ALASFLPHLVFVYWVFFKKSAKNQSKMTAFYRGEGMKWLVTIILVIGCFKLIPALDVV
                     LFFVGYFIALFLNNMIPFVLSKHHH"
     gene            5030..6163
                     /gene="carA"
                     /locus_tag="PWY43_000117"
     CDS             5030..6163
                     /gene="carA"
                     /locus_tag="PWY43_000117"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL2135041.1"
                     /translation="MFKPAILVLTDGSVFSGKSIGADGHTIGEVVFNTAMTGYQEILT
                     DPSYTKQIATLTYPHIGNTGINLEDKESPNVCASGLIIRDLPLLHSNFRASSSLSDYL
                     IQQNIVAIADIDTRRLTRILREKGAQAGCILVGDATEENIANALAFANSFGSMAGKDL
                     AQSVTCNELYKWTDGEWQLGKGYVPQQQTEFHVVAYDYGVKHNILRMLAERGCRLTVV
                     PAKTSAEAVLALNPDGIFLSNGPGDPEPCDYAITAIRKLLETQKPIFGICLGHQLLGL
                     AAGGKTKKMVFGHHGANHPVQDLDSPKVLITSQNHGFEVDESSLPSNVRVTHRSLFDG
                     TVQGIELTDRPAFSFQGHPEASPGPNDVAYLFDRFVSEMKKVK"
     gene            6266..9472
                     /gene="carB"
                     /locus_tag="PWY43_000118"
     CDS             6266..9472
                     /gene="carB"
                     /locus_tag="PWY43_000118"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL2135042.1"
                     /translation="MPKRTDINTILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPNMADVTYIEPIEWKTVEKIIEKERPDAILPTMGGQTALNCALDL
                     SKNGVLKKYNVELIGATEDAIDKAEDRGRFKEAMTKIGLNTPQSFVCHSFEEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNRDEFIAICERGFEASPTHELLIEQSVLGWKEYEM
                     EVVRDRADNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAINPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLNE
                     LRNDITGGLIPASFEPTLDYVVTKIPRFAFEKFANADDRLTTQMKSVGEVMAMGRTFQ
                     ESLQKALRGLETGICGFNLLSEEPEKIRRELANPGPNRILYVADAFGAGFSLEEVHHY
                     SKIDPWFLVQIQDLVLEELALEKKQFSDLDANEIRRLKRKGFSDKRIAQLTKVSEKMV
                     RDYRHTYAILPVYKRVDTCAAEFASDTAYLYSTYEEECEAKPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHASLALREAGYETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RVENPWGVIVHYGGQTPLKLANALSDAGVNIIGTSADSIDAAEDRERFQQILNTLGLK
                     QPANRTARNALEAVALANEVGYPLVVRPSYVLGGRAMQIVYNDEELNRYMREAVSVSN
                     DSPILLDHFLNNAIEVDVDCICDGENVLIGGIMQHVEQAGIHSGDSACSLPAYSLSGH
                     VLDEIRKQTNAMAFALGVKGLMNVQFAVQNEVVYVLEVNPRASRTVPFVSKATGRPLA
                     KIAARVMAGETLADLNALEEIIPSKFFVKEAVFPFIKFPGVDTILGPEMRSTGEVMGI
                     GNTFAEAFFKAQLGAGERIPKVGKVFMSVIEEHKPALIYIAKRFQEQGYGLCATFGTA
                     NFLRQHGIAVQTVNKVREGRPHIVDAIKNGEIALVINTVSGEPEVIADSHSIRRTALQ
                     QRVPIYTTLAGADAISYGVKHINEFDVYSVQELEQKAN"
     gene            complement(9547..10338)
                     /locus_tag="PWY43_000119"
                     /pseudo
     CDS             complement(9547..10338)
                     /locus_tag="PWY43_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            10541..11539
                     /gene="yiaK"
                     /locus_tag="PWY43_000120"
     CDS             10541..11539
                     /gene="yiaK"
                     /locus_tag="PWY43_000120"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDL2135043.1"
                     /translation="MRVSYEELKAQFKRVLLARNVREEIAEECATVFADTTQAGAYSH
                     GVNRFPRFIQQLENGDIVPEAVPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMELAS
                     ENGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
                     IAVPTNPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNRRL
                     MPMGFWKGSGLSIVLDMIATLLSNGESTAAVTEDKDDEYCVSQVYIAIEVDRLIDGKT
                     KDEKLNRIMEYVKTAERADPDVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQAL"
     gene            11664..12137
                     /locus_tag="PWY43_000121"
     CDS             11664..12137
                     /locus_tag="PWY43_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL2135044.1"
                     /translation="MFFGHISQVKANRYPPAIQIALNYLKNTDFDAMEAGVYELKGRQ
                     IYVQVLDLNTKSKHEFQPEVHRNYLDVQYLHRGKEIMAAAVDTGTNPIAMEYNPERDI
                     QYYQSVANENEFRCVEGNFAVFFPEDCHRTAIFDDTETIRKVVVKIAMSEIEEQR"
     gene            12134..12613
                     /locus_tag="PWY43_000122"
     CDS             12134..12613
                     /locus_tag="PWY43_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2135045.1"
                     /translation="MKALAQLVGKLLEILVVVILSAMSILVFVNVVLRYGFNSSISVT
                     EEVSRYMFVWLAFLGAVLAFSENQHVKVTMLTDKLSPTARNILSVVTDLAMLFCCYLI
                     VDGSWIQFNLNLNNFAPISGLPQGITFLASTVAGVLIGLLIIARIVATVGVIAKGEK"
     gene            12614..13891
                     /locus_tag="PWY43_000123"
     CDS             12614..13891
                     /locus_tag="PWY43_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL2135046.1"
                     /translation="MTVVIFLSVLLGSILLGIPVAFALLVCGVALMLHLDLFDAQILA
                     QQIVSGADSFSLMAIPFFILAGEIMNEGGLSKRIIDLPMKLVGHKRGGLGFVAILAAM
                     IMASLSGSAVADTAAVAAMLLPMMKTTGYPIDRSAGLIGTAGIIAPIIPPSIPFIVFG
                     VASGVSITKLFLAGIFPGIMMGICLAALWWWQAKRLDLMTFSKATKEELCISFKNSIW
                     ALLLPVIIIGGFRSGMFTPTEAGAVATFYALIVSLFVYREMKFKDLYKVVLAAAKTTA
                     VVMFLVAAANVTGWLITVAELPMMLTELLEPLIESPTTLLLVIMLAVFIIGMVMDLTP
                     TVLILTPVLMPLVEEAGIDPVYFGVLFILNTSIGLITPPVGNVLNVITGVSKLPFDQA
                     AKGVMPYLVMMICLLLLFVFFPSLVLVPLSWMQ"
     gene            13994..14980
                     /locus_tag="PWY43_000124"
     CDS             13994..14980
                     /locus_tag="PWY43_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2135047.1"
                     /translation="MKLFNLKTLVALVAGVAVMSGSAMAETSLHFGYEAPRSDTQHIA
                     AKKFDELLAAKTKGEIKLKLFPDSTLGNAQTMISGVRGGTIDIEMSGSPNFTGLEPKL
                     NVIDIPFIFKDREHVYKVLDGEVGQGLLKDLEAQGLKGLAFWDVGFRAFSNSKHPVTK
                     PEDIKGLKVRTNQNPMYIQAFTLLGGNPVPMPLSELYTALETRAVDAQEHPIGIFWSA
                     KLFEVQKHLSLTNHGYTPLIVVMNKAKFDGLAPELQTALVEAAKEAGQFQRDLNVKNE
                     KEIIEKLRKQGIEVIEQVDNAPFKAAIEAEIRKAFVEKHGDELLKKVDALAQ"
     gene            15054..16037
                     /locus_tag="PWY43_000125"
     CDS             15054..16037
                     /locus_tag="PWY43_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2135048.1"
                     /translation="MKLFNLKTLAAFVAGFALFSTAQAETSFRFAYEAPRSDTQHIAA
                     KKFNDLLQEKSQKGLKLSLFPDSTLGNAQTAISGVRGGTIDIAMSSSSNFTGLEPKLN
                     VIDIPFIFKDRDHAYKALDGEIGQGLLTALEAHGLKGLAFWEVGFRAFSNSKHPITKP
                     EDIKGLKIRTNQNPMYIQAFSILGANPVPMPLSELYTALETKAVDAQEHPIGIFWSAK
                     LYEVQKYFSFTNHGYTPLIVAINKAKFDALPAEQQTALVEAAKEAGKYQRQLNLENEQ
                     KIIDSLKKAGIEFVDNLDTTPFKDAVSAETRKAFIEKNGDGLVKAIDALAK"
     gene            16168..17652
                     /locus_tag="PWY43_000126"
     CDS             16168..17652
                     /locus_tag="PWY43_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2135049.1"
                     /translation="MQHIIKRFGSHTAVNDVSLDIHSGEIVALLGENGAGKSTLIKIL
                     AGIYDRDEGDILFHNQKIQSAHAIEKGNKKPIAFIHQDLGLIEWMTIAENMAFVMGFP
                     RKFGLIDWAKVREQSQKALDFVGIKLSADTRVFDLSRTEKALLAIARAIAVNAEILVL
                     DEPTASLPASDVEHLFTVLNRLRSQGVGMIYVTHRLDEVIAISDRILVMRDGFPVAEG
                     QTKLYDVKGLVKAIVGEESRGRQRVDLPTDTPEILSLQNVMVGDTGPVNFRLHKGEMI
                     ALAGLRGAGQEEIGRLLFGERTLDKGEIQLDKRSYSAKDPQDAIAKGVALVAGDRTRE
                     SVFMSMTTSENLFINPVLSGHSPLKRYSRREEWGESWYKFQLFDIRPKNLFIDASALS
                     GGNQQKIVLARWMLLNTPVLILEDPTAGVDVGARAEIYDLLNKALAEGVAIIVISTDF
                     EEIAHLCNRALVFNRGEIAGELLNEEVTFANLLALASDSQSKKQ"
     gene            17847..18893
                     /locus_tag="PWY43_000127"
     CDS             17847..18893
                     /locus_tag="PWY43_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2135050.1"
                     /translation="MAQTNIKSTALEQPVSIARDGFMAWFSQMSTRYGLLWLCVLLVI
                     IFSLTTESFASMLTLNAILESKSKIALLALAATTTMIVGKIDLNVGFGIVLWHILVIT
                     LQVQYGFSWYMAILIVLVIAALYGLLNGILVALADIDSFVATLGSGTVLYAVALWHSG
                     GRQIVGDLPDEFIALNSTEIFGIPISAFYVLAVAIVMWLVTEHTPTGRCMYAVGGNPT
                     AARLNGINIKKYTIVPFIVSSVITAFTGVLISAQQGVGQASVGMDYLLPALVGAFLGS
                     TTIRPGRINVWGTVVGIAILAIGISGIQQFGGAFWVEPLFNGATLLLSITIAGYAQRK
                     RLLNQKAVQKKSTN"
     gene            18906..20015
                     /locus_tag="PWY43_000128"
     CDS             18906..20015
                     /locus_tag="PWY43_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2135051.1"
                     /translation="MKKAFRTTLTAIATLTAIAFGNNAFANDDPFVQEAKQLVAAATA
                     KQEKWDGPTTGPQLQKEKTVIFIASDMKNGGVLGVIDGMKEAVGVTGWKLDVLDGAGT
                     VNNQLAALNQAIARKPDAIVIGGWNPNVAKIPLQKATKNGIKLAAWHATPEPGAIDKY
                     GIFYNVTSDSDEIARLSAMLAVAESNGKAKAIILTDSLYEIALRKANVMKEVIEKCKE
                     CKVLEFIDTPLADTSSRMPNLTFSLLQKYGDDLEYALAINDLYFDFMAPSLRTAGKDK
                     PFSISAGDGSVTAYQRIRNGDKQFATVPEPLNLHGWQLVDELNRAFANEKPSGYITPA
                     HLVTKENVAFDGGENNLYAPQNGYKGAYKAIWGVK"
     gene            20120..21010
                     /locus_tag="PWY43_000129"
     CDS             20120..21010
                     /locus_tag="PWY43_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDL2135052.1"
                     /translation="MSNKFIPKISNPRFNELVGNTFELEELFDKAIWAEGPAWSKPQQ
                     ALYFSDVKGCTMYRWTEQDGTQVFRQPSYFSNGNAFDANDNLITCEHGNRGISLTTPS
                     GEYRFLVGQLDGKRLNSPNDVVVKSDGIIWFTDPPYGILSDNEGKQSPSEIIGCYIYC
                     YDPKTDKLNIATFNTMRPNGLAFSSDEKQLFVADMSSVEFDEDGLHHLVVFDVDGNKL
                     KNRRDVAEISPGIPDGFRIKQNGVIFCSCEEGIIALLPDGTELGRFVLGKLTSNCSFG
                     DDEQTLFITCSNSVYRLTIK"
     gene            21082..22551
                     /locus_tag="PWY43_000130"
     CDS             21082..22551
                     /locus_tag="PWY43_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDL2135053.1"
                     /translation="MDNKMNYTLGIDCGGTFIKAALFDTQGNIQALHRENVSVISEQA
                     GYAERDMSQLWAICAEVVRSTIEKSGVNPADIKAVGISAQGKGAFLLDKQNRPLGRAI
                     LSSDQRSLEIVKQWQAEGIPQQLYPITRQTLWTGHPVSILKWVQQNEPERYAQIGAVL
                     MSHDYLRFCLTGELHCEETNISESNLYNMEKGEYDQQLADLLGMPDVLEKLPPVIEPN
                     KISGYVTENAAKLTGLAVGTPVVGGLFDVVSTARCANLDDETKLNAVLGTWSVVSGIT
                     DHIDASQSLPFVYGRYAEAGKFIVHEASPTSAGNLEWFVNQWKLDYVKINQAVAQLPP
                     AASSVLFVPFLYGSNAGLGMQAGFYGMQAHHTQAHLLQAIYEGVLFSLMSHLNRMFIR
                     FPKADVLRVTGGPVKSKVWMQMLADLTGMRLEIPQMEETGCLGAALMAMQGVGLDISQ
                     VQALNNEMQVFEPNPENYAAYQTKYQRYQKLTEALNTMI"
     gene            22761..23072
                     /locus_tag="PWY43_000131"
     CDS             22761..23072
                     /locus_tag="PWY43_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDL2135054.1"
                     /translation="MAKSLDAKREELTNEIRDILKNAEELFNETSDSSTEEYKKLKDK
                     LAKQFDDVKDRFATLKDDTVDSARTVVKQTDTLVQDNPYKAITVAGVVGLLLGVLISR
                     R"
     gene            23152..23547
                     /locus_tag="PWY43_000132"
     CDS             23152..23547
                     /locus_tag="PWY43_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2135055.1"
                     /translation="MLDNIKSGFRNSLITLLEIAQVRGEMARIEINEQKNQLISVLIF
                     ILLAFVFLLVSFVSLLFGLDNYLLPEQKVKVFFSIAIIMFIAVTICGMMILSALKKQR
                     HFMQSTLTELKLDIAAFKSALTLKQNGEH"
     gene            23534..23833
                     /locus_tag="PWY43_000133"
     CDS             23534..23833
                     /locus_tag="PWY43_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135056.1"
                     /translation="MENIKMEELKLTEKELLVLKGNALRMRFKAETNKTKNEIMQPFK
                     QVKQTGLALVNSPAVKTIALDFLVKRLLSMKGISFSVLGLTAFFLLQQKHKRDDS"
     gene            complement(23920..25515)
                     /locus_tag="PWY43_000134"
     CDS             complement(23920..25515)
                     /locus_tag="PWY43_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDL2135057.1"
                     /translation="MQRHEQLAQVQQTQKWDFIIIGGGASGLGIAVDAASRGYKTLLL
                     ESHDFAKGTSSRSTKLVHGGVRYLANGDVALVREALRERGRLAKNAAHLFKNQSFIIP
                     NYKFKDCVMYRVGLGLYDMLSGNLSLGKTENISKTEAKRRLPTLNDTGLRTGTVYQDG
                     QFDDSRLAVNMAQTVVEHGGTVLNHAKVTSLHKTPQGKIDGVTFYDELNQQEHTVFGT
                     AVINATGVFMNDILSMDHGSTKKFVVPSQGIHLVLDKSFLPSNDALMIPKTSDGRVLF
                     AVPWHEVLVVGTTDTLIENPSYEPIPLEQEVNFILETAAQYLTKKPTRDDVKAVFAGQ
                     RPLAAPEKEGQSTKEVSRSHKVLTDATSGLITITGGKWTTYRQMAEDTVEEALKVHSE
                     LGNKPCVTVNLPIHGNIPAAQVNLQDHLYVYGSDIPQLRALMTETPEYAQKIHPRLDY
                     TVAEVIWAVRHEMAQTVEDVLARRVRLLFTDARAAIDSAAKVAAIMAKELGKDEMWQQ
                     QQVIQFLDVAKHYLLVDYQPQPI"
     gene            25873..27318
                     /gene="glpT"
                     /locus_tag="PWY43_000135"
     CDS             25873..27318
                     /gene="glpT"
                     /locus_tag="PWY43_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDL2135058.1"
                     /translation="MFGPFKPAPHIAELPADKVDARYKFLRWQVFAGIFFGYAAYYFV
                     RANFDLAQKGLIEAGMYTKTDLGVIGTAAGLAYGLSKFVMAGMSDRSNPKVFLPTGLL
                     LSGLCMTLMGLMPWATSSILVMWVMIFLNGWFQGMGWPPCGRTMVHWWSKSERGTIVS
                     IWNTAHNLGGMVPGAMVLLASALYFSEYGVEATAKDVWQQALYFPGIAAMIAAIPIYL
                     VMKDTPQSCGLPPVEKWRNDYPDDYNEKTYEHDLSTKEIFVTYVLKNKLLWYIAIANV
                     FVYLIRYGVLKWSPVYLGEVKHFNIKGTAWAYTIYELAAIPGTLLCGWVSDKLFKGKR
                     GLTGFIFMILTTAAVVAFWLNPATPESEIAQYAGHAWYENPYQLMDFIWMTTIGFLIY
                     GPVMLIGLHALELAPKKAAGTAAGFTGLFGYLGGTVAASAVVGWAADKFGWDGGFYVM
                     IGGGVLAILLTFIVMVEEGKHKAKLEDHYGK"
     gene            27388..28461
                     /gene="glpQ"
                     /locus_tag="PWY43_000136"
     CDS             27388..28461
                     /gene="glpQ"
                     /locus_tag="PWY43_000136"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2135059.1"
                     /translation="MKFKFKTLVAALALSATALGAYAQQSDKLVIAHRGASGYLPEHT
                     LESKALAFGQQADYLEQDLAMTKDNRLIVIHDHFLDGLTDVAKKFPERKRADGRYYVV
                     DFTLDEIKTLEMTENFEVKDGKQAQVYPNRFPMWQSHFTIHTLEEELEFIQGLEKSTG
                     KKIGIYPEIKAPWLHHKEGKDIALETLKVLKKYGYDSKTSPIYLQTFDFNELKRIKTE
                     LLPKLGMDVKLVQLVAYTDWHETEEKDKNGNWVNYDYDWMFKDGAMAEVAKYADGVGP
                     GWYMLVDKEKSKVGNIVYTPLVKELAKYKMELHPYTVRKDALPDFFQNVDEMYDALLN
                     KAGATGVFTDFPDTGVEFLKKQK"
     gene            28651..29991
                     /locus_tag="PWY43_000137"
     CDS             28651..29991
                     /locus_tag="PWY43_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2135060.1"
                     /translation="MFSFLKASPPAEQKVEASRVDAEYRKLRWQVFAGVFIGYAAYYL
                     IRKNFSLAMPYLIDEYGFTKADLGTVGVALSLAYGFSKFIMGNVSDRSNPKYFITIGL
                     LGSAIVSLVFGLVPGVLSSIPIMIILAALNGWFQGMGYPPGAKTMTNWFSTSERGVWW
                     SWWNVSHNLGGGLIGPLAILGLSIFGVWQSLFYLPALIAIVLAFVMFYLMRDTPESQG
                     LPPIDEYKGEKVVEKVESAHTLSSKDIFYKYILNNKFLWAIAIANVFVYFIRYGIIDW
                     APTYLKEVKHFSVDKQSWAYFLYEYAGIFGMLASGYLSDKVFKGHRAPPMLLFLAGVL
                     IAIVVYWKNPAGNPLIDNICLVAIGFLIYGPVMMIGLQAADLVPRVATGTATGLTGLF
                     GYLLGSASAGYVMGKLVDMFGWDGGFYALILSCFLAFAFIALTLFKKTSKQLEV"
     gene            complement(30053..31033)
                     /locus_tag="PWY43_000138"
     CDS             complement(30053..31033)
                     /locus_tag="PWY43_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2135061.1"
                     /translation="MKQRRFGKTNKMVSEIGLGTWQLGTKWGEPFNHQEAMAILETAY
                     EQGINFIDTADVYNNGQSEKAIGDILKKYPDFFYVVTKCGRALNPHTAEMYTPQAIEK
                     FVDGSLSHLGTEKLDMILLHCPPTLVYRNDEIFTKLEQLKAGGKLIDYGVSIETVEEG
                     LLAMEYDIAAMEVIFNMFRLKPLEQLFPTAQQKNVGIIARVLLASGLLTGRYNACTQF
                     GKDDHRTFNRNGEAFDKGETFSGVDYQLGLQAVEELKALFGTEDLIPYALRWVLMNEA
                     VSTVIPGASKVSQVITNAAVDNFPPLTENEMQKVEDIYNRLIRPSVHHLW"
     gene            complement(31052..31690)
                     /locus_tag="PWY43_000139"
     CDS             complement(31052..31690)
                     /locus_tag="PWY43_000139"
                     /inference="COORDINATES: protein motif:HMM:NF036826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,4-xylosidase"
                     /protein_id="HDL2135062.1"
                     /translation="MPFSKEMGEVGNGVLKLIGRGSLANTHDLSLIARRWQAFYFDAE
                     TKVKFTPFSYQSMAGLTNYYNHSHWSWIFITKNDQGQQVIEVAENKGGLRNGQYTSYL
                     KDKAIVIPDGTEYVWFKTKVRKETYRYEYSFDGKQWHTIPVELDAAVLSDDYVLRSYG
                     GFFTGAFVGLAAVDYSSYESTAEFSYFDYKELGDKQLTDGSFSWEKSESCFG"
     gene            complement(31693..32739)
                     /locus_tag="PWY43_000140"
     CDS             complement(31693..32739)
                     /locus_tag="PWY43_000140"
                     /inference="COORDINATES: protein motif:HMM:NF016497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2135063.1"
                     /translation="MQIQNPVLPGFNPDPSIVRVGDTYYIATSTFEWFPGVRIHESKD
                     LVHWNLVKNVLDTTTLLDMRGNPSSGGIWAPDLSYAEGKFWLVYTDVKITDGAFKDMT
                     NYLTTAKEIQGPWSAPIKLNGIGFDASLFHDDDGRKYLVQQTWDHREYHHPFDGITLT
                     EFDVATMKLKPETQRTIFEGTEVKLVEGPHIYKINGEYYLFAAEGGTVFTHQEVVARS
                     KTLEAHSFQLAPNTPFITNFDTPNSYLQKQGHGSLVDTPSGEWYYASLTARPWNHATE
                     SATDPRGWSTLGREAAIQKVEFDQDGWPYIVGGHGGKTFVEAPKDAIFTEAPIDHSQH
                     DEFDSSTLDINWNT"
     gene            complement(32762..34124)
                     /locus_tag="PWY43_000141"
                     /pseudo
     CDS             complement(32762..34124)
                     /locus_tag="PWY43_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248294.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(34392..35006)
                     /gene="rsmG"
                     /locus_tag="PWY43_000142"
     CDS             complement(34392..35006)
                     /gene="rsmG"
                     /locus_tag="PWY43_000142"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL2135064.1"
                     /translation="MLAKLNRLLAQAEINLTDQQKEQLVGFVRLLDKWNKAYNLTSVR
                     NPDEMLVKHILDSLVVSRHLQGETFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRI
                     TFIKNALRELGIKNVTPVQSRVEEYTESQFDGVLSRAFASLNDMIDWCYHLPTENGKF
                     YALKGVYDEQEITEIKQPIQLVEVIKLEVPELVGERHLVVLKKP"
     gene            complement(35019..35243)
                     /locus_tag="PWY43_000143"
     CDS             complement(35019..35243)
                     /locus_tag="PWY43_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135065.1"
                     /translation="MIYLRKLGFSPFDHQMFAQPSWYFCNALVHTNYENLRQGVFVTK
                     AYLVMFLENLLFEKSHSLSNRALRINENND"
     gene            complement(35338..37230)
                     /gene="mnmG"
                     /locus_tag="PWY43_000144"
     CDS             complement(35338..37230)
                     /gene="mnmG"
                     /locus_tag="PWY43_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL2135066.1"
                     /translation="MIYNQIYDVIVVGGGHAGTEAALAPARMGLKTLILTHNVDTLGQ
                     MSCNPAIGGIGKGHLVKEIDAMGGLMAKATDVAGIQFRTLNNSKGPAVRATRAQADRV
                     LYRNAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARAVVLTAGTFLA
                     GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQ
                     HGDEVLPVFSFMGDVSQHPRQIPCYITHTNDQTHDLIRNSLDRSPMYTGIIEGVGPRY
                     CPSIEDKVMRFADRNSHQIYLEPEGLNTIEVYPNGISTSLPFDVQMGIVNSMKGLENT
                     RIIKPGYAIEYDYFDPRDLKPTLETKAIDGLFFAGQINGTTGYEEAGAQGLLAGINVA
                     LQVQGKEAWYPTRDQAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTP
                     KAHELGLIDEARWVRFNQKMEAIEQERARLKSTWAHLQMPNLDELNALLANPLAREAS
                     GEDLIRRPEMSYEKLTQIALFAPAIEDKQAAEQVEIAIKYQGYIEHQYNEIERHKRHE
                     NTLIPVEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGM
                     LKRGEL"
     gene            37679..38152
                     /locus_tag="PWY43_000145"
     CDS             37679..38152
                     /locus_tag="PWY43_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDL2135067.1"
                     /translation="MLEKITACPCQTGKRYAECCQPFHLKQTFASTAEQLMRSRYSAY
                     TLVNIPYIVETTVPSQQKLLDQTAMKEWAENTKWVGLSVLNHLPSLSRIHSQVEFKAF
                     FDTDEGIQGHHETSLFVQIDERWFFVDPTVPLPHQKQPCVCGSGKKFKHCCGNYL"
     gene            38224..38853
                     /locus_tag="PWY43_000146"
     CDS             38224..38853
                     /locus_tag="PWY43_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDL2135068.1"
                     /translation="MDFSKILSQVLDTAKDAATNGLIKGNSKNDQIAKIGGGAVAIGL
                     ISTLFGRNGGSSLAKLGSLAAIGSLAYQAYQKYQAQNSSVSELSQNNFENVDENVASK
                     VILQAMIAAAASDGAITEEEKAAIVSQVGDEAEVIEWIRQEMDTPATVEEIARQVGND
                     QALATQVYLASRAVCRDLERKEIVFLANLADALGLAEALVEQLEKDAGF"
     gene            complement(38931..39578)
                     /locus_tag="PWY43_000147"
     CDS             complement(38931..39578)
                     /locus_tag="PWY43_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2135069.1"
                     /translation="MISIRNVSKRYRTNSGWRTVLKDINFELKKGEKVGILGRNGAGK
                     STLIRLMSGVEPPTTGTIERQMSISWPLAFSGAFQGSLTGMDNLRFVSRIYDADFDYV
                     KSFTEEFSELGEYLYEPVKSYSSGMKARLAFALSLSIEFDCYLIDEVIAVGDARFAAK
                     CKHELFEKRKDRSLILVSHSPASIKEYCDSAMVLEEGIMHTFDSIDNAYNFYNKT"
     gene            complement(39575..40372)
                     /locus_tag="PWY43_000148"
     CDS             complement(39575..40372)
                     /locus_tag="PWY43_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2135070.1"
                     /translation="MRYGDNTTFKRSCVIQKRVIHALLMRELITRYGRRNIGFLWLFI
                     EPLLLTLIISLAWKYIKADKYSTLNIVAFMLTGYPIAMMWRNTTNRSKGAISANISLL
                     YHRNVRVLDTIFTRALLEVAGTSIAQILLMAFFIAIGWIDVPHDIFYMLCAWVLMAFF
                     ALGLGLIICALAHYIEPFGRIWGTFSFVLLPLSGAFFFVHNLPSQAQQIALWFPMIHG
                     TEMFRHGYFGETVITYESISYIIVSDLILLFIGLLFVRSFSKGIEPR"
     gene            complement(40372..41475)
                     /locus_tag="PWY43_000149"
     CDS             complement(40372..41475)
                     /locus_tag="PWY43_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL2135071.1"
                     /translation="MSKKKKHFLANPLLWLTVIIPTACSGIYYSLFASDIYVSESSFV
                     VRSPNNQSSLTGVGALLQNSGFARSQDDTYSVQEYMRSRTALEQLEAEIPVKRFYSEQ
                     GDLLSKFNGFGFNDTQEAFYRYFKEHLNIDVDSISGIATLRVRAFNAEEGQKINENLL
                     QQGETLINRLNERARQDTVEFAEERVKEAEKDVNNTALALSQYRTKNKIYDLPAQSGV
                     QLSLISTLKSELIRVETQLAQLQSITPDNPQVAALQMRQKSLKKEIDDQSKQLAGGNT
                     SVATQTAEYQRLVLANELAQQQLSAAMTSLQNTRGEADRQQLYLEVIGKPSKPDWALE
                     PYRIYNIISTFFIGLILFGVLSLLIASIREHKN"
     gene            complement(41547..42722)
                     /locus_tag="PWY43_000150"
     CDS             complement(41547..42722)
                     /locus_tag="PWY43_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDL2135072.1"
                     /translation="MKKTSLFSLFTFSLLSACSSLPTSGPSYSEVLASNENNAETQLP
                     EVNLIKLDNITVQNLYQAQQSQRFSGFDGSIGVGGYAGTVNVGDILEISIWEAPPAVL
                     FGGTFSAEGQGNGHLTQLPQQMVNKNGTVTVPFIGNIKVAGKTPEAIQNQIVGALSRK
                     ANQPQALVRIANNLSSDVTVIRQGNSIRMPLSANNERILDAVAAVGGTSENIEDVTVR
                     LTRGNKVRSMAFETLIADPSQNITLRSGDVVALMNTPYSFTGLGAVGNNQQMRFSSKG
                     ITLAEAIGKMGGLIDDRSDPRGVFVFRHIPFTQLDYDNQAQWREKGYAEGMDVPTVYQ
                     VNLLKPQSMFLLQRFPIQDKDIVYVSNAPLSEFQKFLRMIFSITTPITGTANTIRAY"
BASE COUNT        12514 a         9011 c         9028 g        12276 t
ORIGIN      
        1 gacttaacac cttggtcatc tttttctttc aattcatgta aaacggtttt aaataaattg
       61 atatttaagc caccacataa accgcgatcg gttgaaacta ctaaatagcc aacttttttt
      121 accggacgtg gggtcaaaaa tgggtgttta tactcaatgt tacctttggc aatatggcta
      181 attaccttgc gaatactttc agcataaggg cgagaagccg ccatacgctc ttgggtttta
      241 cgcatttttg atgcggcaac catttccatc gctttggtaa ttttttgtgt attacgaaca
      301 cttgcaattt tggttcttat ctctttagca cctgccatat tatttctccg ctaaaaagtt
      361 agattaccaa gcactgttct ttttgaaacc agataaaatc tcgttcaatt tatctttaat
      421 ttcatcgttg aaattgccgg attttgacag ttcttgcata aactcgccgt agttactttg
      481 agcataagct aataagtttg cttcaaatga gccaacacgc tcaaccggaa tatcatctaa
      541 gtaaccgtat tcagctgctg ctaatgataa acctaattgt gcaacagaca tcggctcgaa
      601 ttgtttttgt ttcaacaatt cagttacttt ttgaccgtgt gaaagctgtt tacgtgttgc
      661 ttcatcaaga tctgatgcga actgggcaaa tgccgctaat tcacggtatt gagcaagtgc
      721 ggtacggata ccaccggcaa gcttcttaac cagtttagtt tgtgcagaac cacctacacg
      781 ggaaaccgag atacccgggt ttaccgcagg gcgaataccg gcattgaata agcctgattc
      841 taagaaaatc tgaccatcgg taattgaaat tacgttagtc ggtacgaagg ctgaaacgtc
      901 acctgcttgg gtttcgatga tcggtaatgc ggttaaagag ccggtttgac ctttcacagc
      961 accgttagtg aaacgttcta catagtctgc atttacacga gacgcacgct ctaataagcg
     1021 agagtgtaga tagaacacat cacccgggaa tgcttcacga cccggtggac ggcgtaataa
     1081 caatgaaatt tgacggtaag caactgcttg cttagacaaa tcatcgtaaa cgatcaacgc
     1141 atcttcacca cggtcacgga agtattcacc cattgcacaa cctgcataag gtgcaagata
     1201 ttgtaatgca gcagattcag acgcagaagc cactacaacg atagtgttcg ctaatgcacc
     1261 gtgttcttct aatttacgca ccacgttggc gatagttgat gccttttgac cgattgcaac
     1321 gtagatacat ttaatgcctg aatcacgttg gttgatgatc gcatcaatcg ctaatgcggt
     1381 tttaccggtt tgacggtcac cgataatcaa ttcacgctga ccacgaccga ttggtaccat
     1441 tgagtccacc gctttgtaac cggtttgaac cggttgatca accgattgac ggtcgataac
     1501 gcccggtgcg atcacttcga ttggtgagaa accgtcattt tggatttcgc ctttaccatc
     1561 gattggctga cctaaggtat ttacaacacg acctaataag ccacgaccaa ccggcacttc
     1621 taaaatacga ccggtacatt gtacttgcat accttctgct aaatcggcat aaggtcccat
     1681 tacaactgca ccaacagaat ctctttctaa gtttaatgca atcgcataac gattgcccgg
     1741 taatgcaatc atttcacctt gcattacatc agataagccg tggatacgaa taaccccgtc
     1801 acttaccgaa acgattgtcc ctgtgctttg agcttcactc accacattaa actgggcaat
     1861 acgtttttta atcaattcac taatttcagt tgaatttagt tgcatttttt attcctctta
     1921 caagctcaac tcttgactca agcggtttag ctgtccacga ctactaccat caatcactac
     1981 atcatcataa cgaatgatca cacccgcaat taatgaacta tctattgatg aagtaatacg
     2041 tactttcgtt ccgagtcgct tttccatagc attggcaatt tttgcttctt gtgcatctgt
     2101 taatttatct gcagaaacaa cctcaacatc tttaattgat tcctgttctg ctcttaattc
     2161 taaaaatgca ttcaataccg caggtaatac tgctaaacgc ttgttatcag ccattacgcg
     2221 aatgaaattt tgcccatatt gatctaattg ttcgccacaa atcttgataa acgtctcaga
     2281 aatctgaccg cttgcaagtg atgagttaag aaaactagct acttgctcat cgttagcgac
     2341 ggcggcagaa aaagttaaca tctgctgcca ttgttccaat tgaccttgtt ctaaagcaaa
     2401 atcgaaagcc gctttagcat aggggcgagc tactgtgctt aattctgaca tctgcccctc
     2461 ctagttatag ttccgcaact agcttatcaa taatatcgtt gttcgcagcg gtatccaccg
     2521 aacgacccac gattttttct gcaccagcaa tagccaatgc agccactttt tgacgaagct
     2581 cttcttgaac gcgtttacgt tcgctttcaa cttccgcgta accctgttca ataatacgtt
     2641 gacgttccac ttcagcttct gcttgtacag attctaaaat ttcattacga cgttttgttg
     2701 ctaaatcaat gatattttgt gcttcttctt tcgctctaag gatttcttga tccgcagccg
     2761 ctttagaatc agcttgttct tgtttcgctt tttcggcaga agccaatgcg ctagcaatat
     2821 ttgcctgacg ctcttctatc gctcgaatta gcggtggcca tacaaacttc atacagaacg
     2881 caacaaaaag tgcgaacgca atcagttgac caattagtgt tgcatttaaa ttcacaacgc
     2941 cctcctaaaa gccggtaaag ttattcaact gaataacggt taatcaaatc ccgacttatt
     3001 tcaataaatc aatgaacggg tttgcgaaga tgaatagtaa tgaaatacct acagcgatca
     3061 ttgcgatagc atctaaaaga ccagcaacga taaacatttt agtttgtaag cttgatgcta
     3121 attccggctg acgtgcagat gactctaaga atttgccacc taagatagca aagccgatag
     3181 cagtaccaag agcagcgaaa gctaataaaa ttgaagcacc aataattgtt gctgtgatta
     3241 ctgattccat aatgttctcc attagattga ggaattagcc cgaaggctgg gttgttgata
     3301 aaaatacata tcaagcggtc aatattttgc aaaaatttgc gaaaaaccta ccgcttgtaa
     3361 ggctagttta atgatccgct ttgttataag cgatactcaa ataaacaatc gttagcatca
     3421 taaaaataaa ggcttgaagc gtaataacta aaatatggaa aatcgcccaa accaagtgta
     3481 atggaatccc caacgcctgt agagcgaagt tgtctgccat atacattact gcaataagga
     3541 taaagattaa ctcacctgca tacatattac cgaataaacg gaacgccaat gaaatcggtt
     3601 ttgccaaaag cgttaccgtt tcaagaataa agtttaccgg aatgaatgcc cagtggttaa
     3661 acgggtgtaa cgtgtattct ttcactaaac cgccaaaacc tttcgatttc accgtgtaaa
     3721 ataaaattaa cgcaaataca caaatagaca tacccagtgt tgcactaata tctgccgttg
     3781 gtactgcacg caaatagtga ataccaaaca gattggctaa ctgtggtaag aaatcgaccg
     3841 ggataaggtc gatcgcattc ataataaaca cccaacagaa aatcgttaat gctaatggtg
     3901 caattacatt gcgaggtccg tggaagttat ccttcactaa accgtttacc cactcaataa
     3961 caatctcaac aaaacattgc aattttcccg gaacgcccgt tgttgcattt tttgctacac
     4021 gcgagaacac ccataaaaaa atcgcacctg ccactaaaga gaagaacagt gtatctaaat
     4081 gcactgccca aaagccttca cctgtcgata aaaaactcaa gtgatggcta atatattctg
     4141 ctgtcgttcc agccataaca tatcctttta aattaaaatt agtggtgatg tttgcttaat
     4201 acaaacggga tcatattatt taaaaataat gcgataaaat aacccacgaa aaacaacaca
     4261 acatctagag cagggataag tttaaaacag ccgatcacca aaataatagt aaccaaccat
     4321 ttcattcctt caccacgata gaaagcggtc atttttgact gattttttgc agatttctta
     4381 aagaaaaccc aatatacaaa cacaagatga ggcaaaaaac tcgctaaagc accgcctaga
     4441 aaggaaaaat taaaattcat tttccataaa gacactccaa gtgcaaaaac gcacaagaga
     4501 aaaaattcaa cctttaatgc ttgtttatat tgtgattttg ctctattgat tacagctgac
     4561 acattttatc ctttttgaat aagtaaacca cacaatcagg ctaataatta gccactgttg
     4621 aatcaaagaa aatccgtgct aaattatact ttacaaatga attgttgcaa ccgaaaagcg
     4681 acacaaaaac ttgttattct tcacattttt gaaaaaaaat tttatttacg ttgcattttt
     4741 ttgagtaaaa ccatcaaaat cagttaacaa aaaaacaaca aatagaggtg tgtgtatttt
     4801 tctctgtcac attatttcaa tatttgataa attccccttg cttaaaaatg aataaaaatt
     4861 cataataatt gcatatattt tcacttccga gtagaaaatg aaataaaaaa agaagaaaga
     4921 aataggcaaa ataactaatt tttacttgaa tgcaaacgtt tgcttgatta aacttagtcg
     4981 cctacttcta aggctacaca gataaaattt gatgattagg agtaaaacaa tgttcaaacc
     5041 agcaattctg gttctcactg acggttctgt cttttccggc aaatccattg gtgctgacgg
     5101 tcacactatc ggtgaagtgg tatttaacac tgcgatgacc ggttatcaag aaatccttac
     5161 cgatccttct tatactaaac aaatagccac cctgacttac ccccacattg gcaataccgg
     5221 cattaattta gaagataaag aatcccccaa tgtttgtgcc tccggcttaa ttattcgtga
     5281 tcttcctctt ctccatagca acttccgtgc gtcctcctca ctttcagatt atctcattca
     5341 acaaaacatt gtcgctattg ccgatattga tacccgccgt ttaacccgaa ttttacgcga
     5401 aaaaggggca caagccggtt gtattttagt aggcgatgcc acagaagaga atattgctaa
     5461 cgctttggca tttgccaaca gtttcggttc tatggctggg aaagatttgg ctcaaagtgt
     5521 cacctgcaat gaattatata aatggacaga tggtgaatgg caattaggta aaggttatgt
     5581 accgcaacaa caaaccgagt tccacgtagt tgcctatgat tacggtgtaa aacacaacat
     5641 tttgcgaatg cttgccgaac gaggctgtcg cttaaccgtt gtgccggcaa aaacctctgc
     5701 tgaagctgtg cttgcgttaa atccggacgg tattttcctt tctaacggcc cgggagaccc
     5761 ggagccgtgc gattatgcca ttaccgcaat ccgaaaatta ttggaaacgc aaaaacccat
     5821 tttcggcatt tgcttagggc atcaactttt aggtttagcg gcaggcggta aaaccaaaaa
     5881 aatggtgttt ggtcatcacg gagctaatca cccggtacaa gatttagaca gcccaaaagt
     5941 gctcatcacc agccaaaacc acggttttga agtagatgaa tccagcttac catctaatgt
     6001 gcgagtgact caccgctcac tgtttgacgg cacggtgcag gggattgaac tcactgatag
     6061 accggcgttt tcgttccaag gtcacccgga agccagcccg gggccgaatg atgttgccta
     6121 tttatttgac cgatttgtca gtgagatgaa aaaagtgaag taaggacgta cctatgtgtg
     6181 tccatcaagc ggtcgaaatt tgcaaaaaac ttaccaaaaa taaccgcttg tagcctgaaa
     6241 taaacgagat aaaagagagc ccaaaatgcc aaaaagaaca gacataaaca ctatcctaat
     6301 tatcggtgcc ggtccgattg tgatcggtca agcgtgtgaa tttgactact ccggagccca
     6361 agcgtgcaaa gcgttgcgtg aagaagggta taaagtggtg ttggtgaact ccaaccctgc
     6421 aaccattatg accgacccga atatggcaga tgtgacttac attgagccga ttgaatggaa
     6481 aacggttgaa aaaatcattg aaaaagaacg ccctgatgcc attttaccga ctatgggcgg
     6541 gcagacagcg ttgaactgtg ccttagatct ttcgaaaaac ggtgtgctga aaaaatataa
     6601 tgtagagctg atcggagcta ccgaagacgc gattgataaa gccgaagatc gtggtcgttt
     6661 taaagaagca atgactaaaa tcggcttgaa tacgccacaa tcattcgttt gccacagttt
     6721 tgaagaggcc ttagcagcac aagagcaggt tggctttcca accctgattc gcccctcttt
     6781 cacgatggga ggctccggcg gtggtattgc ttataatcga gatgaattta tcgccatctg
     6841 cgaacgtggt tttgaagcct cgcctactca cgaattactg attgaacaat ccgtactagg
     6901 ctggaaagag tatgaaatgg aagttgtgcg tgaccgtgcc gataactgca ttatcgtctg
     6961 ctcgattgaa aacttcgacc cgatgggtgt acacaccggc gactccatta ccgttgctcc
     7021 ggcacaaacg ctaaccgata aagagtatca aattatgcgt aacgcttcgc tggcggtatt
     7081 gcgtgagatc ggcgtggaca ccggcggttc aaatgtgcaa tttgcgatta accctgcaaa
     7141 cggcgagatg atcgtcattg aaatgaaccc tcgtgtgagc cgttcttctg cactcgcatc
     7201 taaagccacc ggcttcccga ttgccaaagt tgcggcgaaa ttggctgtag gctacacact
     7261 caacgaattg cgtaacgaca tcaccggagg gctgattccg gcttcattcg agccgacttt
     7321 agattatgtt gtgaccaaaa ttccacgctt cgcctttgaa aaatttgcca atgcagacga
     7381 ccgattaacc actcaaatga aatcggtagg cgaagtaatg gcaatgggaa gaaccttcca
     7441 agagagttta caaaaagcct tgcgaggctt ggaaaccggc atttgcggct ttaacttgct
     7501 ttccgaagag cctgaaaaaa tccgccgtga acttgccaac cccggaccaa accgtatttt
     7561 gtatgttgcc gatgcctttg gagcaggctt ttcattggaa gaagttcacc actattctaa
     7621 aatcgacccg tggttcttgg tgcaaattca agacttagta cttgaagaat tagctttaga
     7681 gaaaaaacag tttagcgatc tagatgctaa cgaaatccgc cgcttaaaac gcaaaggctt
     7741 ctcagacaaa cgtatcgcac agcttaccaa agtcagcgaa aaaatggtgc gggattaccg
     7801 ccatacttac gctattctac cggtgtataa acgggtagat acctgtgcag ccgagtttgc
     7861 ctcagatacg gcttacctct attctactta cgaggaagag tgtgaagcca aaccgtctga
     7921 tcgcaaaaaa gtgatgattt taggcggcgg accaaaccgt atcgggcagg ggattgaatt
     7981 tgactactgc tgcgttcacg cctcgcttgc tctgcgtgag gcaggttatg aaaccattat
     8041 ggtgaactgc aaccctgaaa ccgtttccac cgatttcgac acctccgacc gcctctattt
     8101 cgagccgcta acattagaag atgtactgga aattgtgcgt gtagaaaacc cgtggggcgt
     8161 gattgtgcat tacggcggtc aaacaccact gaaattagcg aatgccttat ctgatgccgg
     8221 tgtaaacatt atcgggactt ccgccgacag cattgatgcc gccgaagacc gtgaacgttt
     8281 ccaacaaatt ctaaacactc tcggtttaaa acagccggca aatcgcaccg cccgtaatgc
     8341 gttggaagcg gtggcattgg caaatgaagt aggctatccg ctggtggttc gcccatctta
     8401 tgtgctaggt ggtcgggcaa tgcaaattgt gtataacgat gaagagctaa accgctatat
     8461 gcgtgaagcg gtatcggttt ccaacgacag cccgatttta ctcgaccact tcctcaataa
     8521 tgcgattgaa gttgatgtgg attgcatttg tgacggcgaa aatgtactca ttggcggcat
     8581 tatgcaacac gttgagcaag ccggtattca cagtggcgat agtgcctgct cactacctgc
     8641 ctattcgtta agcggtcatg ttttggacga aattcgcaaa caaaccaacg caatggcgtt
     8701 tgctctcggt gtgaaaggct tgatgaacgt acaatttgca gtacaaaatg aggtcgttta
     8761 tgtgttggaa gttaaccctc gagccagccg tactgtaccg tttgtcagca aagctaccgg
     8821 tcgcccactg gcaaaaatcg ctgctcgcgt gatggctggt gaaacccttg ccgacttaaa
     8881 tgcattagag gaaattatcc caagcaaatt cttcgttaaa gaagcggtat tcccattcat
     8941 caaattcccg ggtgtggata ccattctcgg gcctgaaatg cgttctaccg gcgaagtgat
     9001 gggcataggt aatacctttg ctgaagcgtt cttcaaagca caactgggtg ccggtgaacg
     9061 aattccaaaa gtcggcaaag tgtttatgtc ggttattgaa gagcataaac ctgctttaat
     9121 ttacattgcc aaacgtttcc aagaacaagg ctacggttta tgtgctacct tcggcactgc
     9181 aaacttccta cggcaacacg gaattgcagt ccaaacggta aacaaagttc gtgaagggcg
     9241 accgcatatt gtcgatgcga tcaaaaacgg cgaaattgcg ttggtaatca acaccgtcag
     9301 cggagagcct gaagtgattg ccgacagcca ctctatccgc cgtaccgcct tgcaacaacg
     9361 tgtgcctatt tacaccaccc ttgcaggtgc tgatgctatc tcctacggcg taaaacatat
     9421 caatgagttt gatgtgtatt cagtgcagga attagaacaa aaggctaact aaaataaagc
     9481 taaaatgaca agcggtcaga ttttgcaaat tttttacaaa ttctgaccgc ttgtgtttgt
     9541 agaggattat ttagtaaatt tatatgctaa atcattccag cgtaattcat ttttgagttg
     9601 tggtagctcg gtattttggt tgatatggat aaactcaata ccaaaatatt cagcgaacat
     9661 tcgtagcata tcggcatcaa tatctaaact aaatacacta tgatgagcac cgccggctag
     9721 aatccatgct tgagtaccta catcaaaatt tggttgaagc ttccaaaaag catgcccaac
     9781 tggtaaattt ggcattggat ttggtgaggc gatagcgtct aaatccgcaa caaccattct
     9841 aaaacggtct cccatatcta caacggttgc attcactgct tttcccgcct tactactgaa
     9901 aattaagcga acaggatctg ccttaccgcc aatacctagc ggtcgaatgg ctaatgttga
     9961 tttctctttg gcaatagacg aacaaacttc aagcatatga gcccccaata ccacttggtt
    10021 ttgttcatct aaatcatagg tgtagtcttc cataaaggaa gaaccgtttg gtaagccata
    10081 acccatcact ttcacttcac gcacaagagc tgcggttttc caacctcctt caccaccaaa
    10141 tccgtagcct tgttccatta aacgctgaac agccaagccc ggtaattgtt taatcccatt
    10201 tagattttgg aaattatcag taaaagcttt aaatccacca ttatctaaga atgtttttaa
    10261 gccgatttca atacgagcag aatatgcgag agattctctt gctgaaccat tttcttgcaa
    10321 gctatcctca acattataca agcggtcaga ttttgcaaaa aaatgaccgc ttgttgccat
    10381 ttctaaagtg tgatctacct ctcaaattta caaaattttt tataacatat tgatttaaaa
    10441 tgttttttat atttttcctt taattgactt taatttgtct taagagtaac ttataaaacg
    10501 tgattttact atttgaaata ttgaggtatt caagatgact attcgagttt cctatgaaga
    10561 attaaaagct caatttaaac gtgttctgtt ggctcgcaat gtgcgtgaag agattgccga
    10621 agagtgtgcc actgtgtttg ccgacaccac tcaagcgggt gcttattcac acggggtaaa
    10681 ccgtttccca cgctttattc agcagttaga gaacggtgat attgtgccgg aagccgtgcc
    10741 gactaaagtg ctttcactgg gggcgattga gcagtgggac gctcaccaag ccatcggtaa
    10801 cttaaccgca aaaaaaatga tggacagagc aatggagctt gcatctgaaa acggcatcgg
    10861 cgtagtggcg ttacgtaatg caaaccactg gatgcgtggc ggttcttacg gatggcaggc
    10921 ggcagagaaa ggctatatcg ggatctgctg gacgaacgcc cttgcggtaa tgccgccttg
    10981 gggggcgaaa gagtgccgta tcgggacgaa cccgttaatc attgcggtgc cgactaaccc
    11041 aatcacgatg gtggatatgt cttgctcaat gtactcatac ggaatgttgg aagtgcatcg
    11101 ccttgctggt cgtcaaacct ttgtcgatgc tggttttgat gatgacggca acccaacccg
    11161 tgatccggca acggtcgaga aaaaccgccg tctaatgccg atgggcttct ggaaaggttc
    11221 gggcttatcg attgtgttag atatgatcgc caccttgctc tctaacggtg aatccactgc
    11281 ggcagtcacc gaagataaag acgatgaata ttgcgtgtcg caagtttata tcgctattga
    11341 agtggatcgc ttaattgacg gcaaaaccaa agacgaaaaa ctcaaccgta ttatggaata
    11401 tgtgaaaacc gcagaacgtg ccgaccctga tgtggcagta cgtttaccgg gccacgagtt
    11461 cacccaaatt ctagccgata acaaagccaa cggcattccg gtggatgaca ctgtttgggc
    11521 gaaattacag gcgttgtaat tgcaaattga ccggtagggt gggcgtaagc ccacgcatta
    11581 gacacaatca taagatgacg tgggcttacg cccaccctac aagcggtcat ttttccgcag
    11641 aattttacaa aacggaggta tgaatgtttt ttggtcatat ttcacaagtt aaagcgaatc
    11701 gatacccgcc ggcgatccaa attgcgttga actatctgaa aaataccgat tttgatgcga
    11761 tggaagcagg ggtttacgaa ctcaaaggca gacaaattta tgtgcaggtg ttggatttga
    11821 acaccaaatc gaaacacgaa tttcagccgg aagtgcatcg caattacctc gatgtgcagt
    11881 atctacaccg aggcaaagag ataatggctg cggcggtgga taccggcacc aatccgattg
    11941 cgatggaata caaccctgag cgggatattc aatattatca atccgttgcc aacgaaaatg
    12001 aatttcgctg cgttgaaggc aattttgcgg tgtttttccc cgaagattgc cacagaacgg
    12061 caatttttga cgacacggaa accattcgca aagtggtggt aaaaattgcg atgagcgaaa
    12121 ttgaggagca acgatgaaag ccttagctca attagtcgga aaactgttag aaatcttggt
    12181 ggtggtgatt ttatccgcaa tgtcgatttt ggttttcgtc aatgtggtac tacgttacgg
    12241 ttttaacagc agcattagcg tgaccgaaga ggtgtctcgc tatatgtttg tttggttggc
    12301 gtttttaggg gcggtgttgg catttagcga aaatcaacac gttaaagtca caatgctgac
    12361 cgataaatta tcgccaacgg ctcgtaatat cttaagcgta gtcacagatt tagcgatgtt
    12421 gttctgttgc tacttaattg tggacggtag ctggattcaa tttaacctca acctgaataa
    12481 ctttgccccg atttcaggct taccgcaagg catcactttc cttgccagca cagtggcagg
    12541 ggtattaatc ggtttattaa ttattgcccg cattgtggca acggttggcg tgattgcaaa
    12601 aggagaaaaa tagatgactg tggtaatttt tctttccgtc ttgcttggct cgattttatt
    12661 gggcattcca gtagctttcg ccctgttggt ctgcggtgtg gcgttaatgt tgcatttaga
    12721 tctgttcgat gcccaaattt tggctcaaca aattgtgagc ggtgcagaca gtttctcgct
    12781 aatggcaatt ccgttcttca ttcttgccgg tgaaattatg aacgagggcg gtctctccaa
    12841 acggattatc gacctgccga tgaaattagt cggtcataaa cggggcggtt taggttttgt
    12901 ggcaatttta gcggcgatga ttatggcaag cctttccggt tcggcggtgg cggataccgc
    12961 agcggtagcc gcaatgctac tgccgatgat gaaaaccacc ggttatccga ttgaccgctc
    13021 tgccggttta atcggtacgg cagggattat cgccccaatc attccgccaa gcattccgtt
    13081 tatcgtattt ggtgtggcta gtggcgtgtc cattaccaaa ctgttcttag ccggtatttt
    13141 cccgggcatt atgatgggga tctgcttggc tgccctttgg tggtggcagg caaaacgttt
    13201 ggatttaatg accttctcca aagcgaccaa agaagaactt tgcatttcat ttaaaaacag
    13261 tatttgggcg ttattgttgc ctgtcattat tatcggtggt ttccgctcgg gaatgttcac
    13321 cccaaccgaa gcgggtgcgg tcgcaacttt ctatgccttg attgtttcac tgtttgttta
    13381 ccgtgaaatg aagttcaaag atttatacaa ggtagtgttg gcggcagcga aaaccacggc
    13441 agtagtgatg ttcttagtgg cagcggcaaa tgtgaccggc tggctgatta ccgtagcgga
    13501 attaccgatg atgctcaccg agctgttaga gccgttaatt gaaagcccga ccacactctt
    13561 attggtgatt atgttagcgg tgtttattat cggaatggtg atggacttaa ccccaaccgt
    13621 gcttatttta acgccggtat taatgccgtt ggtggaagag gcaggcattg acccggttta
    13681 cttcggtgtg ctgtttattc ttaacacctc catcggcttg attacaccac cggtcggcaa
    13741 cgtgctaaac gtgattaccg gcgtgtcgaa actgccgttc gaccaagcgg ctaagggcgt
    13801 aatgccttac ctcgtaatga tgatttgttt actactgttg ttcgtgttct tcccgtcact
    13861 ggtgttagtg ccgttaagct ggatgcagta atggtttgaa ccgaaacaag cggtcgaatt
    13921 gacgcaaaaa tttacaaatt tttttggcaa aacagaccgc ttgcaactca atttttaatc
    13981 acaggagaac cctatgaaat tatttaattt aaaaacctta gttgccttag tggcaggcgt
    14041 tgcggtaatg agcggctctg caatggcaga aacctcactg cattttggtt atgaagcccc
    14101 acgttccgat acccaacaca tcgccgccaa aaaattcgat gaattattgg cagcaaaaac
    14161 caaaggtgaa attaaattaa aactgtttcc ggacagcacc ttaggtaatg ctcaaacgat
    14221 gatcagcggc gtgcgtggcg gtactatcga cattgaaatg tccggctcgc caaactttac
    14281 cggcttagag ccaaaactaa atgtgattga tattccgttt attttcaaag atcgtgaaca
    14341 cgtttacaaa gtattagacg gcgaagttgg tcaaggctta ttaaaagatt tagaagccca
    14401 aggcttaaaa ggcttagcct tctgggacgt tggcttccgt gctttctcca actcaaaaca
    14461 ccctgtgacc aagccggaag acatcaaagg cttaaaagtc cgcaccaacc aaaacccgat
    14521 gtacattcaa gccttcacct tactaggcgg caacccggtg ccaatgccac tttctgagct
    14581 ttacaccgca ctcgaaaccc gtgccgttga tgcccaagaa cacccaatcg gcattttctg
    14641 gtcggcaaaa ctgtttgaag tacaaaaaca tttaagttta accaaccacg gctacacccc
    14701 gttaatcgtg gtgatgaaca aagcgaagtt tgacggctta gctcctgaat tacaaaccgc
    14761 attggttgaa gccgcaaaag aagccggtca attccaacgt gatctcaacg tgaaaaacga
    14821 aaaagagatc atcgaaaaat tacgcaaaca aggcatcgaa gtgatcgaac aagtggataa
    14881 cgccccgttc aaagcagcga ttgaagcgga aatccgcaaa gctttcgtgg aaaaacacgg
    14941 cgatgaatta ttgaaaaaag tggatgcgtt agcacagtaa ttgactttat tttagtctaa
    15001 agctccctcc gtttgggggg agttgttgta tagactctac tataggagag actatgaaac
    15061 tgtttaattt aaaaacttta gccgctttcg ttgccggttt tgcactgttt tcaaccgcac
    15121 aagcagaaac ctcattccgc tttgcgtatg aagctcctcg ttctgatact caacatattg
    15181 cagcgaaaaa atttaatgat ttgctgcaag aaaaatccca aaaagggctg aaattaagcc
    15241 tcttccctga tagcacgctc ggcaacgcac aaacggcgat tagcggtgtg cgtggcggta
    15301 ctattgatat tgcaatgtcc agctcctcta actttaccgg gttagaacca aaactgaatg
    15361 tgattgatat tccgtttatt ttcaaagaca gagaccacgc ctataaagcc ttagatggag
    15421 agattggtca aggtttatta accgcattag aggctcacgg tttgaaaggg ttggcgtttt
    15481 gggaagttgg tttccgagcg ttttccaact ccaagcaccc gatcaccaaa ccggaggata
    15541 ttaaaggctt aaaaatccgt actaaccaaa acccgatgta tattcaagcc ttctctattt
    15601 taggtgcaaa tccggtgcca atgccgcttt ccgagcttta cactgcactg gaaaccaaag
    15661 cggttgatgc ccaagaacac ccaatcggca ttttctggtc ggcaaaactc tatgaagtac
    15721 aaaaatattt cagctttact aaccacggtt acaccccgtt aattgtggcg attaacaaag
    15781 cgaaatttga tgcactgcca gccgagcaac aaaccgcatt agttgaagcg gcaaaagaag
    15841 ccggaaaata ccaacgccaa cttaacctag aaaatgagca gaaaattatc gactcgctca
    15901 aaaaagcagg tattgaattt gtggataact tagataccac accgttcaaa gatgccgtat
    15961 cggctgaaac ccgcaaggca tttatcgaga aaaacggtga tggcttagtt aaagccattg
    16021 atgcattggc gaagtaaaaa tagagctaca aagtgctcgg ttaaactaac ctcacacgaa
    16081 tcgtgtgagg tacaagcggt caaaatccgc aaaatcttta cacatctttc ctcaaaggag
    16141 gaacaaatgg cacagcccat tttaagtatg cagcacatca tcaaacgctt tggtagccat
    16201 acggcggtga atgatgtcag ccttgatatc cactcaggcg agatcgttgc cttactcggt
    16261 gaaaacggtg caggtaaatc gaccctaatc aaaatccttg ccggtattta tgaccgtgac
    16321 gagggtgata ttcttttcca taaccaaaaa atccaatctg cccacgctat tgagaaaggc
    16381 aacaaaaagc ccatcgcttt tatccaccaa gatttaggcc taatagaatg gatgacgatt
    16441 gccgaaaata tggcatttgt aatggggttc cctcgcaaat ttggcttgat tgactgggcg
    16501 aaagtgcgag agcagtcgca aaaagccctt gattttgtag gcattaaact ctctgccgac
    16561 actcgagtgt ttgatttaag ccgtaccgaa aaagccttac tcgccattgc ccgtgcgatt
    16621 gccgtcaatg cagaaatctt ggttttagac gaacctactg cttccctacc tgcctctgat
    16681 gtagagcatc ttttcactgt gttaaaccgc ttacgttccc aaggtgtggg aatgatttat
    16741 gtgacccacc gtttagatga agtgatcgcc atttccgacc gcattttggt gatgcgggac
    16801 ggtttcccgg ttgcagaagg gcaaaccaag ctctatgacg tcaaaggctt ggtcaaagcg
    16861 attgtaggcg aagagagccg tggcagacag cgggtggatt taccaaccga tactcctgaa
    16921 attttaagcc tgcaaaatgt catggttggc gacaccggcc cggtgaattt ccgtctgcac
    16981 aaaggtgaga tgattgccct tgccggcttg cgtggagcag ggcaggaaga aatcggccgc
    17041 ttattatttg gggagcgaac cttagacaaa ggcgaaattc agttagacaa gcggtcatat
    17101 tctgctaaag atccgcaaga tgcgatagcc aaaggtgtgg cattggttgc gggagaccgc
    17161 acccgtgaaa gcgtgtttat gtcaatgact accagtgaga acctgtttat caacccggta
    17221 ctaagcggtc attcaccatt aaaacgctac tcccgccgtg aagagtgggg tgagagctgg
    17281 tacaaattcc aactattcga tattcgtccg aaaaatttgt ttattgatgc cagtgcgtta
    17341 tccggtggta atcagcagaa aatcgtgttg gcacgttgga tgctcctcaa tacgcctgtt
    17401 ctgattttag aagacccgac tgccggcgtg gatgtaggag caagggcaga gatttacgac
    17461 ttgctcaaca aagcccttgc agaaggggtc gcaattattg tgatttccac cgactttgaa
    17521 gagatcgctc atttgtgtaa ccgagcatta gtcttcaacc gaggggaaat tgccggtgaa
    17581 ctactgaacg aagaggtgac tttcgccaat ttgctggcgt tggcatcgga tagtcagtcg
    17641 aaaaaacaat aacttactct cgcccgcttg cgggagagag acagctaaaa atagcgtcag
    17701 gctattttta gcagagagag ggtaaacaag cggtcatttt tcttaaaaaa tattgcgaac
    17761 cctctccctc cgaaagccta acggctttct cctccctctc ccgcaagcgg gcgaggggaa
    17821 aatgcagaat tttaacggag taatttatgg ctcaaacaaa catcaaatca accgcactgg
    17881 aacaaccggt atctatcgcc cgtgacggtt ttatggcatg gttttcacaa atgtccactc
    17941 gttatggctt actgtggtta tgtgtgttat tggtgattat tttctcactt accaccgaat
    18001 ctttcgcctc aatgctgaca ttaaatgcga ttttagaaag taaatcgaaa attgccctac
    18061 ttgctttggc agccacaact acaatgattg tcggcaaaat cgacctgaac gtgggtttcg
    18121 gtattgtgtt gtggcacatt cttgtgatta ctctgcaagt ccaatacggc ttctcgtggt
    18181 atatggcgat cttaatcgtg ttagtgattg ccgctcttta cggcttgctg aatgggattt
    18241 tagttgctct tgctgatatc gatagctttg tggcaacact tggctcaggc acagtgcttt
    18301 atgccgtagc actctggcac tcgggcggtc gccaaattgt cggggattta cccgatgaat
    18361 ttatcgcact caacagcaca gaaattttcg gtatcccgat ttctgctttc tatgtgctgg
    18421 cagtggctat cgtaatgtgg ttggtgacag aacatacccc gaccggtcgt tgtatgtatg
    18481 cggttggtgg caacccaacg gcagctcgcc taaatggtat caatattaaa aaatacacca
    18541 ttgtgccgtt tattgtctct agtgtaatca ctgctttcac aggcgtattg atctctgctc
    18601 aacaaggcgt tggtcaggca agtgtcggta tggactactt actgcccgca ttggttgggg
    18661 cattcttagg gagtaccact attcgcccag ggcgtattaa cgtatgggga acagtggtgg
    18721 ggattgccat tcttgccatc ggtatttcag gtattcaaca attcggtgga gcgttctggg
    18781 ttgagccact ctttaacggt gcaacactgt tactttccat cactattgca ggctatgccc
    18841 aacgcaaacg cttgttaaat caaaaagctg tacagaaaaa atcgaccaac taagaggatt
    18901 tgattatgaa aaaagcattt cgtacaacct taactgcaat cgcaacctta actgctattg
    18961 ctttcggaaa taatgcgttt gccaatgacg acccgttcgt acaagaagca aaacagcttg
    19021 tcgcagcagc tacggctaag caagaaaaat gggacggacc gacaaccggc cctcaattac
    19081 aaaaagagaa aactgtgatt tttattgcct ctgatatgaa aaacggtggt gtacttggtg
    19141 tgattgacgg aatgaaagaa gctgttggcg taacgggctg gaagttagat gtattagacg
    19201 gtgcaggtac ggtaaacaac caactcgccg cacttaacca agcgattgcc cgcaaaccgg
    19261 atgcgattgt gattggtggc tggaacccga atgtggcgaa aatcccacta caaaaagcga
    19321 ctaaaaacgg cattaaactt gcagcttggc acgcaacacc ggagccgggt gcgattgata
    19381 aatacggcat tttctacaac gtgacctctg actcagacga aattgcacgc ttatcggcaa
    19441 tgcttgcggt ggcagaatct aacggtaagg caaaagcgat tattttaacc gactcgctat
    19501 atgaaattgc gttacgcaaa gcgaacgtca tgaaagaagt tatcgaaaaa tgtaaagagt
    19561 gtaaggtgtt agagtttatc gacacgccat tggcggatac ctcaagccgt atgcctaacc
    19621 tcactttcag cttactgcaa aaatatggcg acgatttaga gtatgcgttg gcaattaacg
    19681 atctctactt tgactttatg gctccgtctt tacgcaccgc tggtaaagat aaaccgttta
    19741 gcatttccgc aggtgatggc tcagtaacgg cttatcaacg tattcgcaat ggtgataaac
    19801 agtttgcaac cgtccctgag ccacttaact tacacggctg gcagttagtc gatgagctaa
    19861 accgtgcttt cgctaacgaa aaaccgtctg gttatatcac accggctcac ctagtgacta
    19921 aagaaaatgt ggcgtttgac ggtggtgaaa acaacctgta tgccccacaa aacggttaca
    19981 aaggcgctta caaagcgatt tggggcgtga agtaatgacg taggggcaaa taaatatttg
    20041 cctcgaacaa gcggttgttt tttgcaaaga ttttgcgaaa tttaaccgct tgtcataaaa
    20101 aatagagagg gtaatacaaa tgagcaataa atttatcccc aaaatctcca accctcgttt
    20161 taatgagctg gttgggaata cgtttgaact tgaagagctt tttgataagg caatctgggc
    20221 agaagggccg gcgtggagca agccacaaca agccctctat tttagtgatg taaaaggttg
    20281 cacaatgtac cgctggacag agcaagatgg tacacaggtt ttccgccagc cgtcttattt
    20341 ttctaacggc aatgcgtttg atgcaaacga caacttgatt acctgtgaac acggcaatcg
    20401 aggtatcagt ttaaccacgc caagcggtga atatcgtttt ttggtcggtc aattagacgg
    20461 aaaacgactg aattcaccca atgatgtggt ggtaaaatca gacggcatca tttggttcac
    20521 tgatccacct tacggtattt tgtcggataa cgaaggcaaa cagtcgccaa gcgaaattat
    20581 cggttgttat atttactgct atgatccgaa aaccgacaag ctgaatattg caactttcaa
    20641 cacgatgcga ccaaacggct tggccttctc gtcagacgaa aaacaactct ttgtggcaga
    20701 tatgtcgagc gtggaatttg acgaagatgg cttacaccac ttagtcgtat ttgatgtgga
    20761 cggtaataaa ctaaaaaatc gccgagatgt ggcggaaatt agtcccggca ttccggacgg
    20821 cttccgaatt aaacaaaacg gcgtaatttt ctgctcttgt gaagagggaa ttattgctct
    20881 tttacctgac ggcacggagc tgggacgatt tgtgctgggt aagctaacct caaattgtag
    20941 ctttggcgat gatgaacaaa cactgtttat tacttgttcg aatagcgttt atcgtttaac
    21001 aattaaatag cagaatgcat taagtgcgtc tgttgtagca ttacaagcgg tttctttttc
    21061 aaaatttttt acaaacaaat aatggataac aaaatgaact acacactcgg cattgattgc
    21121 ggtgggactt ttatcaaagc cgctcttttt gatacacaag gtaatattca agcccttcat
    21181 cgtgaaaacg tttcggtaat cagtgaacag gcaggttatg cagagcgtga tatgtcacaa
    21241 ctctgggcta tttgtgcgga agtagtgcgt tcaaccattg aaaaaagtgg ggtaaaccca
    21301 gcggatatta aagcagtagg gatttccgct caaggcaaag gggcgttttt actggataag
    21361 cagaatcgac cgttggggcg tgcgatttta tcttccgacc aacgttccct tgagattgtt
    21421 aaacaatggc aggctgaagg cattccgcaa caactttacc cgattacccg ccaaactctt
    21481 tggacaggac accctgtgtc gattttgaag tgggtgcagc aaaatgaacc ggagcgttat
    21541 gcacaaatcg gggcggtgtt gatgtcgcac gactatttgc gtttttgctt aaccggtgag
    21601 ttgcattgcg aagagaccaa catttccgaa agcaatcttt acaatatgga aaaaggcgaa
    21661 tacgaccaac agttagccga tttgttggga atgccggatg tgttggagaa attaccacca
    21721 gtgattgaac cgaataaaat ttcgggatat gtgactgaaa atgccgccaa attaaccggt
    21781 ttagcggtgg gtacgccggt agtcggtggc ttgtttgatg tggtttctac cgcacgttgt
    21841 gccaatttag atgacgaaac caaactgaat gcggtgctgg gaacgtggtc ggtggtgagc
    21901 ggcattaccg atcatattga tgcaagccaa tcattacctt ttgtttatgg gcgttatgcc
    21961 gaggcaggga aatttatcgt ccacgaagca agcccgactt ctgccggtaa tttagagtgg
    22021 tttgtgaatc agtggaaact cgattatgtg aaaatcaacc aagctgttgc ccaattaccg
    22081 ccggcggcaa gctcggtgct gtttgtgccg tttctgtatg gctcaaatgc aggcttagga
    22141 atgcaagccg gtttctatgg tatgcaagcc caccataccc aagcccatct cttgcaggca
    22201 atttacgaag gagtgttgtt tagcttgatg agccatttaa accgtatgtt tatccgcttt
    22261 ccaaaggctg acgtgctgcg agttaccggc ggaccggtga aatctaaagt ttggatgcaa
    22321 atgttggccg atttaaccgg aatgcggctt gaaataccac aaatggaaga aaccggctgc
    22381 ttgggtgcgg cattaatggc aatgcaaggg gttgggctgg atataagcca agttcaagcc
    22441 ctaaataatg aaatgcaggt gtttgagcca aatccggaaa attatgcggc ttatcagacg
    22501 aaatatcagc gttaccaaaa attaactgaa gcattaaata caatgattta acctgaattt
    22561 atgcttttac aagcggtaat atttggttac ttttttgcaa aaattactgc ttttttgttt
    22621 atttaattta tgaatttaat taaataatct ttaaattaaa ggttgacatt attaaatgtg
    22681 ggctgtattt taataatgaa acataaatca attatgtttt atttttatag attacgttta
    22741 attaaataag gagagcaatt atggctaaaa gcttagatgc aaaacgtgaa gagttaacaa
    22801 acgagattcg cgatattttg aaaaatgcag aggaattatt taatgaaaca tctgattctt
    22861 ctacggagga atataaaaag ttaaaagata aattagcaaa acagtttgat gatgtaaaag
    22921 atcgttttgc aacattaaaa gatgataccg ttgatagtgc aagaactgta gttaaacaaa
    22981 ctgatacctt agttcaagat aatccgtata aagcaattac cgttgccggt gtggtagggt
    23041 tattattagg tgttttaatt tctagaagat aattatctgt aattcgtcat taagtcatta
    23101 agccacgcca tatttggggt ggcttattat ttaatataag gaatggttat aatgttagat
    23161 aatattaaat ccggatttag aaatagtctc attacattat tggaaatagc tcaagtgcgt
    23221 ggtgagatgg caagaattga aattaatgag caaaaaaatc aattaatttc agtgcttata
    23281 tttatcttat tggcttttgt atttttatta gtttcttttg tgagtttatt atttggttta
    23341 gataattatt tgttacccga acagaaagtt aaagtattct tttctattgc cattattatg
    23401 tttattgctg tgactatttg cggaatgatg atactttctg ccttgaaaaa acaacgccat
    23461 tttatgcaaa gtacattaac cgagctaaaa ttagatattg ccgcatttaa gagtgcttta
    23521 actttgaaac agaatggaga acattaaaat ggaagagctt aaattaaccg aaaaggaact
    23581 tctagttcta aaaggtaatg cacttagaat gcgatttaaa gcggaaacca ataaaaccaa
    23641 aaatgagatt atgcagcctt ttaaacaagt gaaacaaaca gggcttgcac tggtcaattc
    23701 accggcagtt aaaaccattg cattagattt tttggtaaaa agattattga gtatgaaagg
    23761 aattagcttt tctgtattgg ggttaacagc attcttttta ttacaacaga aacataaaag
    23821 agatgatagc taatatacaa gcggttaaaa tggtttaaaa agttgcaaat aacgtaattg
    23881 caatattttt atcgatttta accgcttgct tttagcgtat tagattggct gtggctgata
    23941 atctaccaat aaatagtgct tcgcaacatc aaggaactgg ataacttgct gttgttgcca
    24001 catttcatct ttacctaatt ctttcgccat aatagcagcc acttttgctg cactatcaat
    24061 cgccgctctc gcatcagtga agagcaaacg aacacggcga gctaatacat cctcaaccgt
    24121 ttgtgccatt tcgtggcgaa ctgcccagat cacttctgca acggtataat ctagtctcgg
    24181 atggattttt tgtgcatatt ccggggtctc agtcattaag gcgcgtaact gtggaatatc
    24241 tgagccatat acatatagat gatcttgtag attcacttgt gctgccggaa tattaccgtg
    24301 aatcggtaag ttgacggtca cacaaggttt attgcctagt tcagagtgga cttttaacgc
    24361 ttcttctacg gtatcttctg ccatttgacg ataagttgtc catttaccac ctgtaatcgt
    24421 aatcaaaccg ctggttgcat cagtgagaac tttgtgacta cgagatactt ctttagtgct
    24481 ttgaccttct ttttccggtg ccgcaagcgg acgttgaccg gcaaagactg cttttacatc
    24541 atcacgagtt ggtttttttg tcagatattg tgcggctgtc tctaaaataa agttcacctc
    24601 ttgttccaat ggaattggct cataagaagg attttcaatc agagtatctg ttgtccctac
    24661 gactaacact tcgtgccaag gtacagcaaa taacactcgt ccgtcagagg tttttggaat
    24721 cattaacgca tcattacttg gtaagaatga tttatctaat actaagtgaa tcccttggct
    24781 tggtacgaca aattttttcg tgctaccgtg atccatactt aaaatatcat tcataaatac
    24841 gccggttgcg ttaatcaccg cagtgccgaa aacagtatgt tcttgttgat tgagttcatc
    24901 gtagaaagtc accccatcaa ttttgccttg tggcgttttg tgcagtgaag tcactttggc
    24961 gtgatttagt actgtaccgc cgtgctctac taccgtttgt gccatattca ctgcaagacg
    25021 tgagtcatca aattgcccat cttgataaac agtgcctgta cgtaaaccag tatcatttaa
    25081 ggttggtaaa cgtcttttcg cttcagtttt actgatgttt tcagtctttc ctaaactcag
    25141 gttgcctgaa agcatatcgt ataagcctaa accaacacga tacattacac agtctttgaa
    25201 tttgtagttt ggaataataa agctttggtt tttaaataaa tgagctgcat ttttagctaa
    25261 gcgacctcgt tcacgcaatg cttctctcac gagtgcaaca tcgccatttg caagatagcg
    25321 aacgccaccg tggactaatt ttgtgcttcg gctcgatgta cctttcgcaa agtcatggct
    25381 ttctaataat aaggttttat agccacggga agctgcatct accgcaatcc ccaaaccact
    25441 tgcgccacca ccgataataa taaaatccca tttttgcgtt tgttgtactt gagctagctg
    25501 ttcatgtctt tgcatttttt gctccttttg ctcgaattaa aacatttaat cattacttaa
    25561 ttagcataaa cgaaaataaa atgacttcaa agacatttgt ttaaaaatta gatctatgtc
    25621 acaaaatatt tctatattgt actttttatg gttctttctc tgtacataag aagagagttt
    25681 tatcgctaga gatactgatg tttgattttc tttttcaatc attttttgtg gtttaaggta
    25741 aattttttgt gaaatgaatc acatttttat aaaaaataac tttgtcattt tttattttca
    25801 tttattattg cttccctaat ttcctaaaaa ggaattactc agaggtttta ctaagtaata
    25861 ttggagaata ctatgtttgg tccatttaaa cccgctccgc acattgcgga attaccggcg
    25921 gacaaagtcg atgcaagata taaattcttg cgttggcaag tgtttgcagg aattttcttc
    25981 ggctatgccg cttactattt tgtgcgtgca aacttcgact tagcacaaaa aggcttgatt
    26041 gaagctggta tgtatacaaa aaccgatctc ggtgtgattg gtacagctgc cggcctggcc
    26101 tacggtttat caaaattcgt gatggcagga atgtccgacc gctctaaccc gaaagtattt
    26161 ttaccgaccg gattgttact ttcaggtcta tgtatgaccc ttatgggctt aatgccttgg
    26221 gcaacatcaa gcattttagt aatgtgggta atgatcttct taaacggctg gtttcaaggt
    26281 atgggttggc caccgtgtgg acgaacaatg gttcactggt ggtctaaatc tgagcgtggt
    26341 actatcgttt ctatctggaa taccgctcac aacttaggcg gtatggtgcc gggggcgatg
    26401 gtgctattag caagtgcgct ctatttcagt gagtatggtg tagaagcaac ggcaaaagat
    26461 gtatggcaac aagcccttta tttcccggga attgcagcaa tgatcgcagc tattccaatc
    26521 taccttgtga tgaaagatac tccacaatct tgcggtttac cgccagttga aaaatggcgt
    26581 aacgactacc ctgacgacta taacgaaaaa acctatgaac acgatttaag caccaaagag
    26641 attttcgtga cttatgtgct gaaaaacaaa ctgttatggt acattgccat tgcgaatgtc
    26701 ttcgtttact taatccgcta tggcgtattg aaatggtcgc cggtgtactt aggcgaagta
    26761 aaacacttca acattaaagg tacagcatgg gcatatacca tttatgaatt agctgcgatt
    26821 ccaggtacat tactctgtgg ttgggtctct gacaaactgt tcaaaggcaa acgtggttta
    26881 accggtttca tctttatgat tcttaccacc gcagcagtgg ttgcgttctg gctcaaccct
    26941 gcgacaccgg aatctgaaat tgcacaatat gcaggccatg cttggtatga aaacccatat
    27001 caattaatgg acttcatttg gatgaccact atcggtttct taatctacgg cccggtaatg
    27061 ttgattggct tacacgccct tgaacttgca ccgaaaaaag cagcaggtac agcagcaggt
    27121 ttcaccggtt tattcggtta cttaggtggt acagtggcag cctctgcagt agttggctgg
    27181 gcagcagata aatttggctg ggacggcggt ttctacgtga tgatcggtgg tggtgtatta
    27241 gccattctct taaccttcat tgtgatggtt gaagaaggca agcacaaagc aaaattagaa
    27301 gatcactatg gtaaataata aataacttat caaaaggaag gacatttttg ttcttccttt
    27361 tatttttaca ttatatgagg taattctatg aaatttaaat tcaaaacatt ggttgctgca
    27421 ttagcattgt ccgcgacagc attgggtgcc tatgcacaac aatctgataa attagtgatt
    27481 gcccaccgtg gtgcaagcgg ttatttacct gagcatacgc ttgaatctaa agctctagca
    27541 ttcgggcaac aagcggacta tttagagcaa gatcttgcta tgaccaaaga taaccgcctg
    27601 attgtgattc acgaccactt tttagacggt ttaactgatg tggcgaaaaa attccctgag
    27661 cgtaaacgag cagacggtcg ttattatgtg gtggatttca cccttgatga gattaaaacc
    27721 cttgaaatga ccgagaattt tgaggtgaaa gacggcaagc aggcacaagt ttacccgaac
    27781 cgcttcccga tgtggcagtc ccatttcacc attcacactt tggaagaaga gctggaattt
    27841 attcaaggat tagagaaatc aaccggcaaa aaaattggga tctatcctga aattaaagcc
    27901 ccttggttgc accataaaga gggtaaagac attgcactcg aaaccctgaa agtgttgaaa
    27961 aaatatggct acgacagcaa aacctcacca atttacctgc aaaccttcga tttcaacgag
    28021 ctgaaacgga tcaaaaccga attactaccg aaactgggta tggacgtaaa attggtgcag
    28081 ttagttgcct acaccgattg gcacgaaacc gaagagaaag ataaaaacgg caactgggtt
    28141 aattacgact acgactggat gttcaaagac ggtgcgatgg cagaagtggc aaaatatgcc
    28201 gatggtgtcg gcccgggctg gtatatgttg gtggataaag aaaaatctaa agtcggcaac
    28261 attgtttaca cgccattagt gaaagagttg gcgaagtaca aaatggaact tcacccatac
    28321 accgtgcgca aagatgctct gccggacttt ttccaaaatg tcgatgaaat gtacgatgcc
    28381 ctgctgaata aagcgggtgc aacaggcgtc tttactgact tccctgatac gggcgtggaa
    28441 tttttgaaaa agcagaaata gctgattttt aagtgaaaca agcggttaga tttctcaaat
    28501 tttttgcaaa aaaagaagga aatttaaccg attttcgttt tcgctcaaaa taattttaat
    28561 cttgctcaca aattttcaaa aatccacttt ttctgctcca aatgaaagat tatagtagcc
    28621 ataggatttc attcttaaag gagtagtaaa atgttttcct ttctaaaagc ctctccacca
    28681 gcagaacaaa aagtggaagc cagcagagtt gatgcggaat atcgcaaatt acgttggcaa
    28741 gtgtttgcag gcgtctttat cggctatgcg gcatattatt taattcgtaa aaacttctcg
    28801 ttggcaatgc cgtatttgat tgatgaatat ggcttcacca aagcagactt aggtacggtg
    28861 ggcgtggcat tatcactcgc ttacggcttc agtaaattta ttatgggtaa cgtttccgac
    28921 cgttctaacc cgaaatactt tatcactatc ggcttgctcg gttcagcgat tgtgagtctg
    28981 gtgttcggtt tagtaccggg cgtgctttcg tctattccaa ttatgatcat tttggcggcg
    29041 ttaaacggct ggttccaagg aatgggctat ccgccggggg cgaaaacgat gaccaactgg
    29101 ttctcaacct ctgaacgtgg cgtgtggtgg agctggtgga acgtgtcgca caacttgggc
    29161 ggcggtttaa tcgggccgtt ggcgatttta ggcttatcta tttttggcgt gtggcaatcc
    29221 ctgttctatc tgccggcgtt gattgcgatt gtactggcgt ttgtgatgtt ctatttgatg
    29281 cgtgatacgc cggaatctca aggcttaccg ccgattgatg agtacaaagg cgagaaagtg
    29341 gtggaaaaag tggagtctgc ccacacactc tcttccaaag acattttcta caaatatatt
    29401 ttaaacaaca aattcctgtg ggcaattgcg attgccaacg tgtttgtcta tttcattcgc
    29461 tacggcatta tcgactgggc accgacttac ttaaaagaag tgaaacattt ctctgtggat
    29521 aaacagagct gggcgtactt cttgtatgaa tatgccggta tcttcggtat gcttgcaagc
    29581 ggttatttaa gcgacaaagt attcaaaggt caccgtgcgc cgccgatgtt gttattcctt
    29641 gctggtgtgt taattgcgat tgtggtgtac tggaaaaacc cagccggcaa cccgttaatc
    29701 gacaacatct gcttagtggc gattggtttc ttaatctacg ggccggtgat gatgatcggt
    29761 ttacaagcag cggatttagt gcctcgtgtt gccaccggta cagcaacagg tttaaccggt
    29821 ttattcggct acttgttagg ctctgcaagt gcaggttatg tgatgggtaa actggttgat
    29881 atgttcggct gggacggtgg tttctatgcc ctgatccttt cctgtttctt agcctttgcg
    29941 tttattgcat tgactctgtt taagaaaacc tctaaacagt tagaggttta aggctaaaat
    30001 tgacaaaaat ggaactcaag ttagagttcc attttttata gcgttggata aattaccaca
    30061 aatgatgcac acttgggcga attaagcggt tgtaaatatc ttccactttt tgcatttcat
    30121 tttcagtgag tggagggaaa ttatccaccg ctgcgttagt aattacttgt gaaaccttac
    30181 ttgctcccgg gattactgta ctcacagctt cattcatcaa tacccaacgt agggcgtaag
    30241 gaattaaatc ttccgtgccg aatagggctt tcaattcttc caccgcttgt aaaccgagtt
    30301 gataatccac acccgaaaag gtttcgcctt tatcgaaggc ttcaccattg cggttaaatg
    30361 tgcggtggtc gtctttgcca aattgggtgc aggcattata tctgcctgta agcaaaccac
    30421 ttgccaacag tacacgggcg ataataccca catttttttg ctgagcggtt gggaagagtt
    30481 gctccaatgg ttttaagcgg aacatattga agataacttc cattgccgca atgtcatact
    30541 ccattgccaa caagccttcc tctaccgttt cgatactcac accgtagtct attaattttc
    30601 cgccggcttt aagctgttcc aatttcgtaa aaatctcatc attgcgataa accaacgttg
    30661 gcgggcagtg gagcaaaatc atatccaact tttccgtgcc aagatggctt aaactaccat
    30721 caacaaattt ttcgattgcc tgtggcgtgt acatttcagc agtatgcggg tttaacgcac
    30781 gtccacattt ggtaacgaca tagaaaaagt cagggtattt tttcaaaata tcaccaatgg
    30841 ctttctcgct ttgcccgttg ttatacacat cagcggtatc aataaaattg atgccttgct
    30901 cataagcggt ttctaaaatt gccatcgcct cttgatgatt gaacggttca ccccatttcg
    30961 tcccaagctg ccaagtgcct aacccaattt ctgacaccat tttgttggtt ttaccaaatc
    31021 tgcgttgttt cattgtgata ttctcctcat tttagccaaa gcaactttca gatttttccc
    31081 aagagaatga gccgtctgta agttgcttat cgcctaactc tttatagtca aagtagctaa
    31141 attctgctgt agattcataa ctggagtaat ccactgcagc cagcccgaca aaagctcctg
    31201 tgaagaaacc gccataactg cgtaacacat aatcatctga aagcactgcc gcatctaatt
    31261 caaccggaat agtgtgccat tgtttgccat cgaatgaata ttcataacgg taagtttctt
    31321 tccgaacttt agtcttaaac caaacatact ccgttccatc aggaatgaca attgctttat
    31381 ctttaaggta agaagtatat tgcccatttc tgagtccacc tttattttct gcaacttcaa
    31441 tcacctgttg tccttgatca tttttggtga tgaaaatcca agaccagtgt gagtgattgt
    31501 aatagttagt taaacctgcc atagattgat aagaaaacgg tgtaaatttc actttggttt
    31561 ccgcatcaaa atagaaggct tgccaacggc gtgcgattag cgataaatcg tgtgtatttg
    31621 caagagagcc tcgcccaatc aattttaaaa caccattacc aacttcgccc atctctttac
    31681 taaaaggcac tcttaagtgt tccagttaat atctaacgtt gagctatcaa actcatcgtg
    31741 ttgagaatga tcaataggag cttcggtaaa aattgcatct tttggggctt ctacaaaggt
    31801 ttttccaccg tgtccaccca caatataagg ccagccgtct tgatcaaatt ctactttttg
    31861 aatggcggct tcacgcccta atgttgacca accacgagga tcggttgcgg attctgttgc
    31921 atgattccaa ggtcttgctg tgagggaagc gtaataccat tcaccacttg gggtatcaac
    31981 taatgaaccg tgcccttgtt tttgtaggta agaattaggt gtatcaaaat tagtaataaa
    32041 cggtgtattt ggagccagtt ggaaagagtg agcctccaat gttttagaac gagccactac
    32101 ttcttgatgc gtaaatactg tgccgccttc cgccgcaaac agataatatt cgccattaat
    32161 tttataaata tgtggacctt ccaccaattt cacttctgta ccttcaaaaa tcgtacgctg
    32221 ggtttcaggt ttgagtttca ttgtggcaac atcaaattcc gttaacgtaa taccgtcaaa
    32281 ggggtgatga tattcacggt gatcccaagt ctgttgcact aaatattttc gcccatcatc
    32341 atcgtgaaac aacgaagcat caaacccaat accattcaat ttaatcggag ctgaccaagg
    32401 accttgaatt tctttggcag tagttagata gttagtcata tctttgaatg caccgtctgt
    32461 aatttttaca tcggtataaa ccaaccaaaa tttaccttcc gcataagaaa gatcaggagc
    32521 ccagatacca cctgaagagg gattgcctcg catatctaac aacgtggttg tatctaagac
    32581 atttttaact aaattccaat gcactaaatc tttcgattcg tgaattctca ctcccggaaa
    32641 ccattcaaaa gtagaagttg caatgtagta agtatcaccc acacgcacaa tagatggatc
    32701 tggattgaaa ccgggtaaaa ctggattttg aatttgcata atctattctc ctatactcaa
    32761 attatttgga ataagcagta attttttcgt tgatatgatc aatatcagta tcgcttaacg
    32821 gatagcgact catcacaaat cctgcaataa tcgctaaaat aattggagcc caaatcattg
    32881 aaagattgat accaaataaa gcctcttcgg tttgagccaa aagcgagccg ttaaaaccat
    32941 aaaccgcatt aatataaccc ggaataatac cgcctaacgc caagccgatt ttaaagaaca
    33001 agcccattaa cgcattaata atacctgcta cacgtttttt cgacttaaac tctccaaagg
    33061 taacaacctc aggcactaac gcccacataa aaccggtagc agaagttaat ccccattgtt
    33121 gtaagaaacg accgatatag cctaaggttg gagagtattt gaaggagtca aattgccata
    33181 cccatagaca aagttgccct agaacgaaaa tcccaaggat agagcctaat aaaccgacgg
    33241 aggcaatcca ctcagaatgc ccaatattat attttaggta aaaaggccac accgtatttc
    33301 cgaagaacat aaaagtaaaa cctattaaga agaataaccc taaaatctgt aatggacgat
    33361 tgcgtttgag ttcattaact aaatctgaat acttgacttt atctgtttct tccgctgttg
    33421 gaataatccg ctctttagta ccaaaataag agagcattaa cgccacaaat ccaattacca
    33481 tatagaccgc ataaacctta aaccaagcag ggcctgcagc agcagacata taatcaccta
    33541 aatagagctc aataccgaaa aagccggtgt tttttaattc ggcgtgtggt gcaagtaact
    33601 acacaaaaag tgggaataag gtataaacaa acaaattagc gatatttgcc attgtcatac
    33661 gggttgtggt taatttagca atttcgtctg tgtctcgagt aagagaagca gaaagcgagc
    33721 cataaggaat attaacccat cgcagtggca aaataagaaa ggaaagcata aatcactgag
    33781 ccacgtactg attcaagagg aagaaatagt agtgaagcta aaaccacaag aggaatccca
    33841 aatttcagta aatacgggcg gtatttaccc tgtgctgagg gcttgctttt gtcaataaag
    33901 gcaccgacaa acggatccca aaacacattt ccccatttta caattaagaa aatcgttact
    33961 cctgctacgg cagaaatgcc gtttacagta gttacgtaag tgagtaagaa accaccaact
    34021 gtaccaaaaa ctaaattttg ggctaagtcg ccactaccat aagcaatacg ttcaaacgta
    34081 ctgagtttag caaactcatc tgccttattt gttaccctgt tcattacgaa cctcctcgtt
    34141 atataattaa gttcgcttat tttcaggtaa agaaataagg gcttcaatta cgaatatttt
    34201 ttactacatt atgttttttt gtttttgtga agcacatcac aaaatagaaa taaatcagat
    34261 gaaataatag gttataaaaa aagcacacct tattaaccag gtatgcttta tgaaaaatag
    34321 ttaacacaag cagtcagatt tgcaaaaaaa tttacaaatt cgaccgcttg tatcattaaa
    34381 cttccaatgt attagggctt tttcaacact actaaatgcc gttctcctac caactcaggt
    34441 acttcaagct taatcacttc cactagctga atcggttgtt tgatttcagt tatttcctgc
    34501 tcatcataca cgccttttaa tgcataaaat ttaccgtttt cagtcggtaa atgataacac
    34561 cagtcgatca tatcattaag cgaggcaaag gctcggctta atacgccgtc aaactggctt
    34621 tcagtgtatt cctccactcg actttgcact ggggtaacgt ttttaatacc aagctcacgc
    34681 aacgcatttt tgataaaagt gatgcgtttg cctaagctgt ctagtagcac gaactgttta
    34741 tcagggttag caatcgccag cggaatgccc ggtagccccg gccccgtgcc gacatcaata
    34801 aacgtttcgc cttgtaaatg acgactaaca acaaggctgt ccaaaatatg tttaaccaac
    34861 atttcatcag ggttgcgaac ggaagtgagg ttatacgcct tattccattt atccaataat
    34921 ctcacaaaac caactaactg ctctttttgt tgatcggtca aatttatttc ggcttgagca
    34981 agcaagcggt ttaatttagc taacattttg caaattcctt aatcattatt ttcattaata
    35041 cgcaatgccc gattacttaa cgaatgactt ttttcaaaca ataaattttc aagaaacatc
    35101 actaaatacg ctttagtcac aaatacacct tgtcgcagat tttcataatt ggtatgaaca
    35161 agtgcattgc agaaatacca cgaaggttgg gcaaacattt ggtgatcgaa cggcgaaaaa
    35221 ccgagtttac gcagatatat cattaaaaat acggcggtag ttcgggtatt tccttctcca
    35281 aagaatacaa gcggtcaatt ttgcaaaaat ttctgcaaaa ccaaccgctt gtagtattta
    35341 cagctcaccc cgtttcaaca ttccttgctt cttcaaattc accaacagaa tggaaatcgc
    35401 tgccggtgtg atgccggaaa tgcgacttgc ctgcccgatg gagacagggc ggtgttgcat
    35461 caatttagct cgcacttcgt tggagaggct ttcgactttg tcgtagtcaa actctaccgg
    35521 aatcagggtg ttttcgtggc gtttatggcg ttcgatttcg ttgtattgat gctcaatata
    35581 gccctgatat ttgatcgcaa tttccacctg ttctgccgct tgcttgtctt caatagcagg
    35641 ggcaaatagg gcgatttggg tcagcttttc gtaactcatt tcaggacggc ggattaagtc
    35701 ttcaccgctt gcttcacggg ctaaaggatt agccaataac gcgttcaatt cgtccagatt
    35761 tggcatttgc aaatgtgccc aagtggattt caaacgagct ctttcttgct caatcgcttc
    35821 cattttttgg ttgaaacgca cccaacgtgc ctcgtcaatt aatcctagtt cgtgggcttt
    35881 tggtgttaaa cgaatgtccg cattgtcttc acgcagcaac agacggtatt cggcacgaga
    35941 ggtaaacaca cggtatggct ctttggtgcc taatgtacag aggtcgtcca ccaacacgcc
    36001 ggtgtaggct tgatcacggg ttggatacca cgcctcttta ccttgaacct gtaacgctac
    36061 gttaatccct gccagcaaac cttgcgcacc tgcctcttcg taaccggtcg tgccgttaat
    36121 ttgtccggca aagaataaac cgtcaatcgc tttggtttca agggttggtt ttaaatcacg
    36181 cgggtcgaag tagtcgtact caatcgcata gcccggtttg atgatgcggg tgttttccaa
    36241 gcctttcatt gagttgacga tccccatttg cacatcaaac ggcaagctgg tggagatccc
    36301 gttcgggtaa acctcaatcg tatttaagcc ttccggttca aggtagatct ggtggctatt
    36361 acgatcggca aaacgcatta ctttatcttc aatagacggg cagtaacgtg gaccaacccc
    36421 ttcgataata ccggtgtaca tcgggctgcg atcaagactg ttgcgaatta aatcgtgggt
    36481 ttgatcgtta gtgtgggtga tgtagcacgg aatttggcgt gggtgctgtg agacatcgcc
    36541 cataaaagag aagacaggta acacctcatc gccgtgctgt ttggcaagta cgtcaaagtt
    36601 gatggtacgg gcatcgaggc gaggtggtgt acctgtttta aggcgatcaa cacgcaaatt
    36661 cagatcacgc agacgatctg ccaacattgt tgcagcagga tcgccggcac gaccacctgc
    36721 atagttatcc aacccaatgt ggatcttacc cgctaagaac gtaccagcgg ttaaaactac
    36781 cgcacgggct ttaaacgtta agcccatttt ggtaactgca cccactgcac ggttgttttc
    36841 caccaaaata tccactactt cttgctggaa aatatctaaa tttggttgat tttctaacgc
    36901 agtgcgtacc gcattacggt aaagcacacg gtcagcttgg gcacgggtgg cacgcaccgc
    36961 aggccctttt gagttgttta gggtacggaa ttggatcccg gcaacgtctg ttgcttttgc
    37021 cattaaaccg cccatcgcat cgatctcttt aaccagatgg cctttgccga tccctccgat
    37081 agccgggttg cacgacattt gccctaaggt atcaacattg tgggttaaaa tcagggtttt
    37141 taagcccatt cgtgccggtg ctagggccgc ttctgtgccg gcgtgaccac cgccgaccac
    37201 gatcacatcg taaatttggt tgtaaatcat atctcttcta ctatcttaca aattctgctg
    37261 aaaaatgagc gcatatttta gcgtaaaaag ggatcatctg ggaaatagat ttttagagag
    37321 gttaagatct aaatgcgaca ctaaatataa tatagatcta tataaagatc tttattctta
    37381 ctcttattag atcctaattt ttctgtgaac aaaagatctt ttcatttaaa aacaataagt
    37441 tagtataatt taaataggtg aatactatca attaaccaat aaattaacct gtggataaaa
    37501 tcttaagtta tccacaatct atagaataaa ggctttttaa agcaatgaat acccaaaaaa
    37561 ttcacgattt ttgcataagt tatccacaag gggtatgtca cgctttttta taaaatttat
    37621 ttttctttat ttatagcgta aacttttcac atctttattt ttatgcggaa atgtaaaaat
    37681 gttagaaaaa atcaccgctt gtccttgcca aacaggcaaa cgctacgctg aatgttgcca
    37741 acctttccat ttaaaacaga catttgcatc aacggcagag caattaatgc gctcccgtta
    37801 cagtgcttat actttggtta atattcccta tattgtggaa acgacagtgc caagtcagca
    37861 gaaattactt gatcagactg caatgaaaga gtgggctgaa aatacaaaat gggtaggatt
    37921 atccgtgcta aatcacttac cttctctttc gagaatacat agtcaggttg aatttaaagc
    37981 cttttttgat acagatgagg ggatacaggg acaccacgaa acctctcttt ttgttcaaat
    38041 tgatgagcga tggttttttg tggatcctac cgtacctttg cctcatcaaa aacaaccttg
    38101 tgtatgtggc tccggaaaga agtttaagca ctgttgtgga aattatcttt aatttgaatt
    38161 gaactcttgt ttgaatctaa ggactaatga gtaaatttca tttacttacc aagaggaaca
    38221 aaaatggatt tcagtaagat tttaagtcaa gtattagata cagcaaaaga tgccgctaca
    38281 aacggattaa ttaaaggtaa ttctaaaaac gatcagatcg ctaaaattgg tggcggcgct
    38341 gtcgctattg gtttaatttc aacgttattt gggcgtaatg gcggttcaag tttagcaaaa
    38401 ttaggctctt tagccgctat cggtagcctt gcttatcaag cataccaaaa gtatcaagcg
    38461 caaaattcat cggtttcaga acttagccaa aataattttg aaaatgtaga tgaaaatgtg
    38521 gcaagtaaag tgattttaca agcaatgatt gccgctgctg cttcagatgg tgctattact
    38581 gaagaggaaa aagcagctat tgtttcccaa gttggagatg aggctgaagt cattgagtgg
    38641 attcgtcaag aaatggacac tccggctaca gttgaagaga ttgcccgcca agtaggtaat
    38701 gaccaagcat tggctacaca agtttactta gcttcccgtg cagtatgcag agatttagaa
    38761 cgcaaagaaa tcgttttctt agctaattta gctgatgctt taggtttagc tgaggcatta
    38821 gttgaacaat tagaaaaaga cgcaggtttc taattttcag ttttagtaac aagcggttat
    38881 attttgagag ttttttgcga aaaacttgtg aaatataacc gcttgttctt ttatgtttta
    38941 ttgtagaaat tataagcgtt atctatagaa tcaaaagtat gcatgatacc ctcttctaaa
    39001 accattgcag aatcgcaata ttctttaatt gaggctgggc tatgagaaac taaaatcaat
    39061 gaacggtctt tccgtttttc aaaaagctcg tgcttacatt tggcagcgaa tcgagcatcg
    39121 cctactgcaa tcacttcatc aattaagtaa caatcaaatt caatagaaag cgataatgca
    39181 aaggctaaac gggccttcat accagaagaa tagcttttta ccggttcata taaatattcg
    39241 ccgagttctg aaaattcttc ggtgaaagat tttacatagt caaaatcagc atcataaatt
    39301 cggctgacga aacgaagatt atccattccg gtcaaactac cctggaatgc accactaaat
    39361 gccagtggcc aagaaatcga catttgacgt tcaatcgtac ctgttgttgg aggttctact
    39421 ccgctcatta aacggattaa ggttgatttt cctgcaccat tgcgccctaa aatacccact
    39481 ttttcacctt tttttagctc aaagttaata tctttaagaa cggtacgcca accgctattt
    39541 gttctataac gcttactgac atttctaata ctaatcatct aggttcgatt cctttactaa
    39601 aacttctcac aaagagtaag ccgatgaata acagaataag atcgctgact ataatataac
    39661 taatgctttc ataggtaatg acagtttcgc caaaatagcc atgtctgaac atttctgtac
    39721 cgtgaatcat tggaaaccac agtgcgattt gctgtgcttg tgatggtaag ttatggacga
    39781 aaaagaatgc tccggacaat ggaagtaata cgaagctaaa ggttccccaa attctgccaa
    39841 atggttcaat ataatgtgct aatgcacaga taattaagcc taaacctaat gcaaaaaatg
    39901 ccattaatac ccaagcacaa agcatataga aaatatcgtg gggtacatct atccagccaa
    39961 tagcgataaa aaacgccatt aataggattt gagcaataga tgtccctgca acttccaata
    40021 aagctcgagt gaatatggta tctaatacac gaacgtttcg atgataaagc aggctaatat
    40081 ttgcggatat agctccctta gatctatttg ttgtatttct ccacatcatt gcaatcggat
    40141 agccggtaag cataaatgca acaatattaa gcgtagaata tttatcagct ttaatgtact
    40201 tccaagctaa tgaaatgatt agagttaata ataagggttc aataaacagc cataaaaagc
    40261 caatatttct acgcccataa cgggtgatca actctcgcat taaaagagcg tgaatcactc
    40321 gtttttgaat cacgcaggaa cgtttaaagg ttgtattatc accatatcgc attagttttt
    40381 atgctctctg atacttgcta ttaacaaact taatactcca aatagaatta agccgataaa
    40441 aaaagttgat ataatattat aaattctgta gggttctaat gcccaatccg gtttgcttgg
    40501 tttaccaatt acctctaaat aaagttgctg gcgatcagcc tctcctcttg tattttgtag
    40561 cgatgtcatt gccgctgaaa gctgttgttg agctagctcg ttagctaaaa ctaaacgctg
    40621 atattcagct gtttgcgttg ccacagaagt attacctcct gcaagttgtt tagattggtc
    40681 atcaatttct tttttcaaac ttttttgacg catttgtaat gcggctacct gaggattatc
    40741 cggcgtaatt gactgcaatt gtgctaattg tgtttcaacc cgaattaatt cactttttaa
    40801 ggtagaaatt aatgagagct gaacgccgga ttgtgcaggt aaatcataaa ttttattttt
    40861 ggttcgatat tggcttaatg ctaacgcagt attattgaca tctttttcgg cctcttttac
    40921 gcgttcttct gcaaactcaa ccgtatcttg tcttgctctt tcattaagac ggttaattaa
    40981 tgtttctcct tgctgtaata aattctcgtt aattttctgc ccttcttcgg cattaaaagc
    41041 acgaacgcgt aacgtcgcaa tacctgaaat agaatcgaca tcaatattca gatgttcttt
    41101 aaagtaacgg taaaacgctt cttgggtatc gttaaatccg aaaccgttaa atttgcttag
    41161 taaatcacct tgctcactat aaaaacgttt taccggaatt tctgcttcaa gttgctctaa
    41221 tgcggtacgg gaacgcatat attcctgaac tgaataggta tcatcttgag aacgggcgaa
    41281 accggagttt tgcaataaag cacccacgcc ggttaagctg gattgattat tcggtgaacg
    41341 aaccacaaag ctagattctg aaacataaat atcagaggca aataaggaat aataaattcc
    41401 ggaacaagca gttggaataa ttacagttag ccacaacaaa gggttggcta aaaaatgttt
    41461 tttcttcttt gacataaaat cctcaacgaa aagtgttaca agcggtcatt ttttaatgat
    41521 tttttaccat tttatctatg agctaattag tacgctctaa ttgtattagc tgtacctgta
    41581 attggtgtag taatggagaa aatcattctc aagaattttt gaaattcaga cagtggagca
    41641 ttcgacacat atacaatatc tttatcttga atagggaaac gctgcaataa aaacattgat
    41701 tgcggtttta ataaattcac ctgataaaca gttggaacgt ccattccctc tgcataacct
    41761 ttttcacgcc attgagcttg gttatcgtaa tctaattgtg taaaaggaat atggcggaat
    41821 acgaacacac cacgagggtc tgaacgatca tcaattaatc cccccatctt acctatagct
    41881 tcagcaagtg taatcccttt acttgagaaa cgcatttgct gattatttcc cacagccccc
    41941 aagccggtaa agctataagg cgtattcatt aaggcaacaa catcgcctga acgtaatgta
    42001 atattttgtg agggatcggc aattaaggtt tcaaacgcca tcgaacgtac tttatttcca
    42061 cgtgttaaac gaaccgttac atcttcaata ttttcgcttg taccacctac tgccgcaacg
    42121 gcatctaaaa tgcgctcgtt atttgcgctt aatggcatac gaatgctatt tccttggcgg
    42181 ataacagtaa catcagacga caagttattc gcaatacgaa ctaatgcttg aggctgattg
    42241 gccttacggc ttaatgcacc aacaatttga ttttgaatag cttcaggcgt tttccctgcc
    42301 actttaatgt taccgataaa aggcacagtt accgtaccat ttttgttcac catttgctgt
    42361 ggtaattgag ttaaatgccc attaccttgc ccttctgcac taaatgttcc accaaatagc
    42421 acggcaggtg gcgcttccca aatggatatt tccaatatat cgcctacatt aactgttccg
    42481 gcataaccac ccacaccgat tgagccatca aagcctgaaa aacgttgaga ttgttgcgct
    42541 tggtataagt tttgtacggt gatgttatct aacttgatta aattgacttc aggtaattgt
    42601 gtttcagcgt tattttcatt acttgctaag acttcgctat agctcgggcc ggacgttggt
    42661 aggctggagc aggctgatag taagctaaaa gtgaatagac taaacaaaga tgtttttttc
    42721 attagatacc tcattacatt tgatagtagt atagcactaa tgttaagcta ggcgtagctt
    42781 aacttatcaa tactatttaa tagaaacaat aggttgtatt tttgtattt
//
LOCUS       DANKBV010000004        42257 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.004, whole genome shotgun sequence.
ACCESSION   DANKBV010000004 DANKBV010000000
VERSION     DANKBV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 42257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42257
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.004"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..542)
                     /locus_tag="PWY43_000151"
     CDS             complement(<1..542)
                     /locus_tag="PWY43_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter MurJ"
                     /protein_id="HDL2135073.1"
                     /translation="MSKKLLRSGMIVSFMTLISRILGLIRDIVVATILGTGVSADIFL
                     FANRIPNFLRRLFAEGAFSKAFVPVLAEYNADNDPNKTREFIAKVSGTLGGLVTVVTL
                     VAMIASPVVAALFGTGWFLDWLYDGPNAEKFTQASFLLKITFPYLWFITFVALSGAVL
                     NTIGKFGVMAFSPVLLNVAI"
     gene            806..1069
                     /gene="rpsT"
                     /locus_tag="PWY43_000152"
     CDS             806..1069
                     /gene="rpsT"
                     /locus_tag="PWY43_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL2135074.1"
                     /translation="MANIKSAKKRAVQSEKRRQHNASQRSMMRTFIKKVYAAVTAGDK
                     AAAQTAFVEMQKVVDRMASKNLIHANKAANHKSKLAAQIKKLA"
     gene            complement(1182..1577)
                     /gene="mscL"
                     /locus_tag="PWY43_000153"
     CDS             complement(1182..1577)
                     /gene="mscL"
                     /locus_tag="PWY43_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL2135075.1"
                     /translation="MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPP
                     IGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQNVFDFLIIALAVFGMVKVINSL
                     KKAPVVEEAPAEPTAEEKLLTEIRDLLKK"
     gene            complement(1710..2237)
                     /gene="hemG"
                     /locus_tag="PWY43_000154"
     CDS             complement(1710..2237)
                     /gene="hemG"
                     /locus_tag="PWY43_000154"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDL2135076.1"
                     /translation="MKTLILYFTTDGQTKKIAERLADVITHEVELISLKDQAVDFAEK
                     IANADQIVIGASIRYGHFNPLVYQFVEQHYPALNAKKTAFYGVNLTARKDNRNTPETN
                     TYVRKFLAKVKWKPTHIEVIAGALLYLRYKPFDRLMIRFIMKITKGETDTSKEYEYTD
                     WQQVENFGKLLANQL"
     gene            complement(2238..3722)
                     /locus_tag="PWY43_000155"
     CDS             complement(2238..3722)
                     /locus_tag="PWY43_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HDL2135077.1"
                     /translation="MQFFSIIRIVGILVMCFSFTMLVPATVALIYGDGGGRAFLEAFA
                     LNFMVGTLLWWLCRDNKNKLRSREGFLIVVLFWVVLGLLGAVPFIILENPDLNVAESI
                     FESFSGLTTTGATVITGLDSLPKAILFYRQFLQWLGGMGIIVLAVAIIPLLGIGGMQL
                     YRAEMPGPLKEQKMRPRIAETAKALWLIYLSLTILCAVAFWLAGMTVFDAISHSFSTV
                     SIGGFSTHDASIGYFNNAAISYITVFFLLLSACNFALHFAVIDRIRDAQSRKKGGILS
                     RLYWGDYEFRFFLIVQLGLFLVCTLVLVGYGYFDNTSVTIEQALFQSVSISTTAGFTT
                     NDFSQWSSFLPTLLVLSSFIGGCAGSTGGGLKVIRVLLLYLQSKREIHRYIHPNVIQP
                     IKLGNHVLSERVVDGIWAFFSAYFFVFVICWLATIACGMETFDALNAVIASLNNLGPA
                     LGSVSSNFTQVPDSAKLVLTFAMVCGRLEVFTLLVILSPVFWKD"
     gene            complement(3726..4337)
                     /locus_tag="PWY43_000156"
     CDS             complement(3726..4337)
                     /locus_tag="PWY43_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL2135078.1"
                     /translation="MEFFIPKADTIFEEEIKKSRFITYLRHTEGMEQAKAFWQELKIQ
                     HPHARHWCWATVAGLPNDSQQYGFSDDGEPSGTAGKPMLNYLLGSGLGEITAVVVRYY
                     GGIQLGTGGLVKAYGNGVQQALLQLERIRKVLRKNYRLQCEYEQFNTISHLISEKDIV
                     IVEQLFSDKVDLLLAFNPSEVEAFADELTQRFSGKLALIKEEI"
     gene            4452..5405
                     /gene="fmt"
                     /locus_tag="PWY43_000157"
     CDS             4452..5405
                     /gene="fmt"
                     /locus_tag="PWY43_000157"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL2135079.1"
                     /translation="MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGK
                     KLQASPVKQLAEAHNIPVYQPKSLRKEEAQAELKALNADVMVVVAYGLILPEAVLNAP
                     KYGCLNVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMLMDVGLDTGDMLHKVTTPIEP
                     NETSASLYAKLAELAPLALLEVLNGLANHTFKAEKQDEALSNYAEKLSKEEAKLDWQL
                     PAVQLERNIRAFNPWPFSFLTLTVDGVEQSIKIHQATVLPHQEKAAGTVINVDKTGIQ
                     IATQQGVLNITQLQPASKKPMSVQDFLNGRADWFVSGTQLS"
     gene            5418..6896
                     /gene="modF"
                     /locus_tag="PWY43_000158"
     CDS             5418..6896
                     /gene="modF"
                     /locus_tag="PWY43_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDL2135080.1"
                     /translation="MQTQPSLTITNARFSLVKQHKLTIDALQIQPNDFWVVVGGNGSG
                     KTAFSLALQDKISLYSGDYGNSFQHIQLLSFEQQQKMLDQIFKDRNNDSVSPDDFGLT
                     ARQVILNGSPKEALCEEYAHKLHIQHLLERPFMHLSTGESRKVLFCQMLVSEPDLFIL
                     DEPFEGLDQQSVQYWQQLIAELQSKMALVLIVNRFNDIPDAANHIALLDNLSLILQGE
                     RAEIEQQAVYSQLKFAEQNVNVPLPESAAPLNSLPENINPFELEKVTIQYGEKKILDN
                     LSWTVEPKQHWWIKGPNGAGKSTLLSIITGDHPQSYANKVRLFGKQRGSGETIWDIKK
                     NIGYVSSQLHMDYRVNCSAMEVILSGFFDSIGVYQQVPNALQLKAMEWLERLNLSNVA
                     KKPFRSLSWGQQRLLLITRAMVKHPSILILDEPLQGLDGINRKLVKQFIEQLVTNSQT
                     QLLFVSHQDSDAPNCITHLFEFVPKGETNEFGFEYHQTALGA"
     gene            6897..7487
                     /locus_tag="PWY43_000159"
     CDS             6897..7487
                     /locus_tag="PWY43_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HDL2135081.1"
                     /translation="MNFLTHSLISLEIDTQTKQQTLYGNFAGDFYKGLIPHLNLADNL
                     KSGVVLHRSIDKISDRPDSLTTQLLQGTFGRYKGIVSDIMIDHFLAKHFQRLFAKDLN
                     EVQAQIFQQIEQQRTIFPEQFVPMFNWLQSRKALTHYADLDFLEQVFLGMSSRVKKGE
                     ILRNAVTEIWQNYTLLEEAAIQEFFTVKDRATQELS"
     gene            7665..8615
                     /locus_tag="PWY43_000160"
     CDS             7665..8615
                     /locus_tag="PWY43_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005697612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2135082.1"
                     /translation="MCLLSLIFDIMTGPSLLPFNEVWKALFQLGEIEETTKVIVYELR
                     LPMAIMALVVGAALGVGGAEIQTLLNNPMASPYTLGLAAAAGFGASIVIAFGGFGLPT
                     EISVPVGAFVMTLFSSAILFAFSSLGRFSSAILVLVGIALLFLFQSLLSLVQFISAPE
                     VSQQILFWLFGNLTKATWQTISIAFGVTTVCILLLFKDAWKLTALRLGEARALSLGIN
                     LTWLRMKTLLIVSVMTATCISFVGIIGFIGLVAPHIARMLLGEDQRFFLPGSMLVGAA
                     FLSMSSVLSKSLVPGALFPVGIITALVGVPFFFWIVLAKR"
     gene            8625..9230
                     /locus_tag="PWY43_000161"
     CDS             8625..9230
                     /locus_tag="PWY43_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2135083.1"
                     /translation="MLNLNDVTIKRGNLVVADKINLHFEQGKIYTLLGPNGAGKSSLL
                     KTLFGEIAHQGTIDFDGNKLTSSHLHSWRKEIGYMPQDTQVEASLSALEVILLGQLDS
                     LNMYISDELLNEAVDIMDKLGISHLAHQDIMRLSGGQRQMVMFAQVLLRKPHILLLDE
                     PVSALDMYHQINLLEYVNAYTRANNLITIMVLHDLSLAAQV"
     gene            9230..9409
                     /locus_tag="PWY43_000162"
     CDS             9230..9409
                     /locus_tag="PWY43_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135084.1"
                     /translation="MLLSEGKIHAQGRACDVLECDIIQRLYNVNIEILYDGNGSPIIR
                     PLRKCLNIDCCLNRK"
     gene            complement(9471..10577)
                     /locus_tag="PWY43_000163"
     CDS             complement(9471..10577)
                     /locus_tag="PWY43_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2135085.1"
                     /translation="MKKLLLNSVFAATLLAFGATANAEIKTITDVLGREVKVDVPAKR
                     VALTFYYPDYIAVTGVENFDKVVGISREFWEKFNPGSWALFAEKMPNLKNIADLGYVN
                     SGTFSTEKTIALKPDVLVLPEIQYQALASEIPRIEQAGIPVVVVDFNKQTVENHTKSV
                     RVFGQLTGTEERAEKIAKEYEEGIADIQSCIDNAKVSKPKIYAEFGNKGPKEHSFTFG
                     KNMWGAIAETVRGDNISAPFVENWGQINPEQVLVSKPDVIMISGTELGNDTNPEIMSM
                     GINIEEADAQKRLKGFIERTGWKELPAVKSGRVYGLYHTASRSISDLASAQFMAKALY
                     PELFKDIDPEKTYLDFHNNYLPITPKGTFFMQLK"
     gene            complement(10764..11771)
                     /gene="lipA"
                     /locus_tag="PWY43_000164"
     CDS             complement(10764..11771)
                     /gene="lipA"
                     /locus_tag="PWY43_000164"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDL2135086.1"
                     /translation="MATSTAQTTATATVKKMSPFKMEKGVKYRDAAKTSVIQVRNIDP
                     DQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFNHGTATFM
                     IMGAICTRRCPFCDVAHGKPLPLDPDEPRKVAETVQDMKLKYVVITSVDRDDLPDRGA
                     AHFSATVREIKALNPHCKVEILVPDFRGRVEQAVAILKENPPDVFNHNLENVPRLYRE
                     VRPGADYKWSLELLKIFKQEFPHIPTKSGLMVGLGETNEEILEVMQDLRDHGVTMLTI
                     GQYLQPSRHHLKVERYVPPAEFDMFREEAEKMGFEHAACGPFVRSSYHADLQAKGELV
                     K"
     gene            complement(11786..12439)
                     /gene="lipB"
                     /locus_tag="PWY43_000165"
     CDS             complement(11786..12439)
                     /gene="lipB"
                     /locus_tag="PWY43_000165"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDL2135087.1"
                     /translation="MKLIIRQLGIQPYEEIWHQMQSFTDSRDENTDDEIWLVQHPAVF
                     TQGSAGKPEHLLNPTNIPVVQSDRGGQITYHGEGQQIMYVLIDIKRLKSLGKEVSVRD
                     LVTALEQTVVQTLAEYGIESYPKADAPGVYVDGKKICSLGLRIRKGCSFHGLALNINM
                     DLTPFQNINPCGYAGLEMCQLSELVHRPDLRCEEVAPKLVAHFSTILGYNNQQIINN"
     gene            complement(12501..12800)
                     /locus_tag="PWY43_000166"
     CDS             complement(12501..12800)
                     /locus_tag="PWY43_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDL2135088.1"
                     /translation="MSQPKAINLQDLPQAKLKDLLEFPCHFTFKVVGANREDLVDDVV
                     VVTQKYAKGDYNPRQQKSSKGTYNSVSIDIVAENIEQVETLYTELAKINGVRMVL"
     gene            complement(12968..14146)
                     /locus_tag="PWY43_000167"
     CDS             complement(12968..14146)
                     /locus_tag="PWY43_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL2135089.1"
                     /translation="MKKTLVKAMSVVGFAVSSIAYADLNTNFNLSAPQINAQTYILMD
                     YNSGEVLVSLNPDQRQYPASLTKMMTSYVVGEALKSGKVQNSDMVIVTENSWAQKFPG
                     SSLMFLDLNSKVSVGELMKGLIIVSGNDASVALAEHVSGSQAAFINEMNKYAQQFGLK
                     NTHFTTVHGLDDPEQYSSARDMAIIGSHIIRDQPEEYKIYAEKEFQYNIKKPQPNRNG
                     LLWDKTIQVDGMKTGHTDKAGYNLVASAINPNTRLISVVMGVPTYKGREVESKKLLQW
                     GFANFETIKSIPAAQSVAEQSVYYGEVNKVQLGSVQDSFVTIPKGRSADLKARYELEK
                     KNLEAPLAKGQVVGKMIYQLDGKDVASTNLQVLQDVPEAGIFGKAWDWIALTVKSLFD
                     "
     gene            complement(14160..14819)
                     /locus_tag="PWY43_000168"
     CDS             complement(14160..14819)
                     /locus_tag="PWY43_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HDL2135090.1"
                     /translation="MRLGKIVTLLLAGFLTFSSLNSLAATKNTKAKSSILAKKQATSK
                     AIKKVGISKVKKKEVVAKATKKKTISTVTTKPIKSTKKLARKVVKKTVTAKKKLVKKQ
                     HKPLVKKHFQTGVASYYANYFNGRRTANGETFNNAKMTAAHRTLPFGTLIEVTNLRNG
                     RSVVVRVNDRGPYAHARVLDLSSAAAKKIGMHHSGTARVKIAIVDKSKLGSTNQFYVM
                     N"
     gene            complement(14911..16038)
                     /gene="rodA"
                     /locus_tag="PWY43_000169"
     CDS             complement(14911..16038)
                     /gene="rodA"
                     /locus_tag="PWY43_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDL2135091.1"
                     /translation="MNSGFKKLFWKIFSLDLLLLSGLLAITGYGLLVLYSASGANERM
                     FTSRLIQVSLGLGLMLIMAMVPPRFYQKVSPYLYIFCLILLILVDLVGETSKGAQRWL
                     NLGFVRFQPSEIAKLAVPLMVATYLGKRPLPPSFKDTFIALAIIIVPTLLVAIQPDLG
                     TSILVCSAGIFVLFLAGLSWKLIGAGVVFLAGFIPIMWFFLMHDYQKTRVMTLIDPNK
                     DPLGAGYHIIQSKIAIGSGGINGKGWMEGTQSQLEFLPEPHTDFIFAVLSEEHGMIGV
                     LILLAIYLFIIARGLVIGTKSDSAFGRILSGGTALLFFVYVFVNIGMVSGILPVVGVP
                     LPLFSYGGTSYVTLMAAFGLMMSTYVHRKRVGSNNPYDKLK"
     gene            complement(16031..17980)
                     /gene="mrdA"
                     /locus_tag="PWY43_000170"
     CDS             complement(16031..17980)
                     /gene="mrdA"
                     /locus_tag="PWY43_000170"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL2135092.1"
                     /translation="MFGKLAKFTASNQREKVRDGQAESNLYARRALVAFLGVLALSGV
                     LLTNLYHLQVVNYEDYQTRSNGNRIKLLPVPPARGLIYDRNGKVLAENLTYFGLFIVP
                     EKTENIEQTLTELKDIVGLTDEDIENYHKEKKRSSRYTPIMLKGNLNEAQMARFAVNQ
                     YRFPSLDVQAYYKRNYPYGELLTHIIGYVAKINENDKKKLQEAGKFGNYAGSHDMGKL
                     GIEKFYEDQLHGVTGFEEVEINNRGKVIRKLRDQAGVAGSSIRLSIDVELQQYVMSLI
                     AKHKGAVVVLDPRDSSILAMVSNPSYDNNLFVGGISGQDYKALLDEPTRPLYSRTTQG
                     TYPPASTIKPFMAVAGLEEGMITPSTTISDPGYWILPGTTQRYRDWKRSGHGATNVNK
                     AITESSDTFFYQLAYKMGIDKMSDWMKKFGFGVKTGIDIFEESAGVMPTREWKQKRYK
                     KPWVQGDTIPVGIGQGYWISTPLQLAKATAVLINNGRVNTPHLMLEVQSSNSVTPYQD
                     PLLYEDIKGVPDVYWNLAKQGMYNVNHAANGTARKVFAGAFYHSAGKTGTAQVFNLNG
                     KNYDKNAIKKELHDHAWFIGYAPYEQPKIVVALILENAGGGGSAAAPVARQIMDYALR
                     KGIGEQPDSQNSEQNKPLVTEEHHE"
     gene            complement(18004..18471)
                     /gene="rlmH"
                     /locus_tag="PWY43_000171"
     CDS             complement(18004..18471)
                     /gene="rlmH"
                     /locus_tag="PWY43_000171"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL2135093.1"
                     /translation="MKIQLIAVGTKMPAWVTTGFEEYQRRFPKEMPFELVEIPAGKRG
                     KNADTKRILELEGKAMLAAAGKAKIVTLDIPGKAWTTEQLATQLEGWKNDGRDVALLI
                     GGPEGLSPECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTINHPYHRE"
     gene            complement(18548..18862)
                     /gene="rsfS"
                     /locus_tag="PWY43_000172"
     CDS             complement(18548..18862)
                     /gene="rsfS"
                     /locus_tag="PWY43_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL2135094.1"
                     /translation="MEQNLVEFLTKTLDDLKAQDILAIDVKGKSSITDTMILATGTSS
                     RHVASTAERLITEAKQAGFEVFGDEGKAVADWIVVDFGKAMVHLLQSDSREMYQLEKL
                     WA"
     gene            complement(19030..19899)
                     /locus_tag="PWY43_000173"
     CDS             complement(19030..19899)
                     /locus_tag="PWY43_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL2135095.1"
                     /translation="MFKKYFGEIILFLVTFIAAAGWFFSKHALVGLPPIGFMGGRFIL
                     ASLILLPFSYRELYRLDKVQVKYSVITGLTYGIYTIFWVLGLAFSTVFGEGAFLVSLA
                     MLIAPLLSWLIFKHTPPKIFWLALPIALSGLYFLSAEKGIFHFSLGSTIFLVSSVFGA
                     LYFVLNNQYAKQVSLFPLITIQLAIVGAVCSLYSYFMESWHFPIPTEVWLWFAASVLI
                     ATNLRFLMQTLGQKYCHIGTSALIMLLEPVWTLLLSVMLLGEQLSATKWLGCSLILIA
                     LIIYRLPILRLKK"
     gene            complement(19950..21134)
                     /gene="uxuA"
                     /locus_tag="PWY43_000174"
     CDS             complement(19950..21134)
                     /gene="uxuA"
                     /locus_tag="PWY43_000174"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2135096.1"
                     /translation="MEQAWRWYGPNDPVSLDDVRQAGATGIVTALHHIPNGQVWSVEE
                     INKRKALLAEKGLAWSVVESVPVHEEIKTQTGNYQQWIDNYKQTLRNLASCGIDTVCY
                     NFMPVLDWTRTDLEYEMPDGSKALRFDQVAFAAFELYILKRPGAEATYSSAEQAEAKA
                     YFEQMSDADIAKLTKNIIAGLPGSEEGYTLEEFQAQLDRYQGLSAEKFRTHLAYFLNE
                     IVPVAQEVGIRMAIHPDDPPRPILGLPRIVSTIEDMQWFVETQPLAANGFTFCTGSYG
                     VLAENDLVKMAEQFADRIYFAHLRATQREENPQSFHEADHLAGDVDMFGVVKALLTEE
                     YRRKANGDHRLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLRGLAEFRGLELALKKVYF
                     ND"
     gene            complement(21153..21902)
                     /locus_tag="PWY43_000175"
     CDS             complement(21153..21902)
                     /locus_tag="PWY43_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HDL2135097.1"
                     /translation="MAAEDLRSYKKIGQELKAELLSGAYKVGDKLPPERDLSNYFNVS
                     RTVIREALIMLELENLIEVRKGSGVYVINLPKSNAEETEELPDVGPFELLQARQLLES
                     SIAEFAAIQATRADVLHLKEILERERRTLEHGDEDYVADEDFHKAIAEITQNEVIIRM
                     QKDLWEYRTNSPMWKGLHSHIPDQSYRTLWLKDHENIIAAIQRKDPVLARKMMWQHLE
                     NVKQKLFELSDLDDPNFDGYLFNVSPLAVNF"
     gene            22138..22983
                     /locus_tag="PWY43_000176"
     CDS             22138..22983
                     /locus_tag="PWY43_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2135098.1"
                     /translation="MNVSKNHNFKDKVVVITGAGGVLCAFFAQEIAKTGAKVALLDIN
                     LESAEKYAQAIVQNGGAAKAYKTNVLDLESIQQTRNAINKDFGTCDTLINGAGGNSPK
                     ATTDNEFHEFNLTDSTKSFFELDKSGIEFVFNLNYLGTLLPTQVFAKDMIGKKGASII
                     NVSSMNAYTPLTKIPAYSGAKAAISNFTQWLAVYFSRVGIRCNAIAPGFLVSNQNRGL
                     LFDNEGNPTPRANKILTNTPMGRFGEANELIGGILFLMDENYSSFINGVVLPIDGGFS
                     AYSGV"
     gene            22993..23808
                     /locus_tag="PWY43_000177"
     CDS             22993..23808
                     /locus_tag="PWY43_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2135099.1"
                     /translation="MTIFTPKHNFIVCIDSDGCAMDTMNIKHEECFGPSAVKVFDITD
                     RDRFIEIWDRINLYSQTRGINRFKGLVISLEEYGYDKNFSQLKHWVNTTSELSNRSLI
                     QAIQEHPSHDLQLALMWSEKVNIGIKTLHTQDRPFRLVKRSLAIIKEFADIAVVSSAN
                     NEAIIDEWTRHGLLPFVDLVYGQDEGTKTHCLNQLSQYGYQPNHILMVGDSPGDLNSA
                     NEAGVNFFPILCGDEDNSWNQLVLETLGKLVYQKFTLQYQSQLIAKFNANLTA"
     gene            23849..25609
                     /locus_tag="PWY43_000178"
                     /pseudo
     CDS             23849..25609
                     /locus_tag="PWY43_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609746.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
     gene            25637..27037
                     /locus_tag="PWY43_000179"
     CDS             25637..27037
                     /locus_tag="PWY43_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2135100.1"
                     /translation="MSSVQRPFGLKDKLAYMAGDIANDLSFMMSAFFLMLFYTNVLQI
                     EGYVIGILFLVSRIIDAFTDVGMGRLVDTIKPFKEGRFRGLLRRTSPFVCIFGFLMFL
                     HVVKDWAYEAKLIYVCVTYIIWGSFAYTAVNIPYGSMATVISNKPEDRASLSVFRAIG
                     ANIALLFISLCIPLVIYQEVDGKQVIQPEMFTIVMGVFMVIAFLLYQYCWLNTCERVQ
                     LPEKEGRRHNNADCLSDVKSIFTSLFSNRPLMIFILVAIILLLANLLIGTMNPYLYVD
                     YFNNKLGLSIGGMLPVIASFVVAPFAQRIVKRFGKKESAAIALLFTGVVYLALFFLKL
                     ENVWIYLATALIALLGLNYFMVVIWAFITDIIDAQYLKTNKREDGTIYAVYSFARKIG
                     QALAGGLGGFALSYVGYVAKAPSQSQETLNAVYNVATLVPAISCIIIFLLLKFVYPLS
                     KEVVDNNVAELEKRHH"
     gene            27099..28502
                     /gene="uxaC"
                     /locus_tag="PWY43_000180"
     CDS             27099..28502
                     /gene="uxaC"
                     /locus_tag="PWY43_000180"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2135101.1"
                     /translation="MKQFLCEDFLLSTPTAQKLYHDYAKDQPIFDYHCHLNPKEIAEN
                     RQFNDLAEIWLEGDHYKWRAMRSAGVEERLITGDADKYSKYLAFAQTVPKCIGNPIYH
                     WTHLELRRPFGITDTLFSPQTAEKIWHQGKALLQQPEFSARGIMQQMNVKLVGTTDDP
                     IDSLEYHKAIAEDSTFDIEVVPSFRPDRAFKIELPLFNDYIEQLGNVADIEINSFDQL
                     KQALLKRIEHFDQQGCKSADHGIEIVRFAAIPDETKLTHILQKRLQNQPLAEEEIAQF
                     STALLVWLGTEYHKRNWVMQMHIGAIRNNNSRMFKLLGADSGFDSIGDRTFAEPLSRL
                     LDSMDQTDQLPKTILYCLNPRDNEMLGSMIGNFQTGGIAGKIQLGSGWWFNDQKDGME
                     RQLQQLSQLGLLSQFVGMLTDSRSFLSYTRHEYFRRILCEMIGGWVERGEAPNDLNLL
                     GNMVKGICYGNAKRYFK"
     gene            28512..29421
                     /locus_tag="PWY43_000181"
                     /pseudo
     CDS             28512..29421
                     /locus_tag="PWY43_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            29509..30147
                     /locus_tag="PWY43_000182"
     CDS             29509..30147
                     /locus_tag="PWY43_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2135102.1"
                     /translation="MRYTTEQIIEKLRQLKVVPVIALDKAEDILPLAKTLSENGLPVV
                     EITFRSEAAEEAIKLVKANYPEVLIAAGTVLTKEQVLTAKNAGADCIVTPGFNPTIVK
                     YCQVLGLPVTPGVNNPMAIEAALELGIQAVKFFPAEASGGVKMIKALLGPYGNLQIMP
                     TGGISPQNINDYLAIPNVVACGGSWFVEKSLIKEQKWEEIGKLTKEVVELVK"
     gene            30371..31822
                     /locus_tag="PWY43_000183"
     CDS             30371..31822
                     /locus_tag="PWY43_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL2135103.1"
                     /translation="MKKIVSTLLILTSNLAFAETIPAQLQEKMDNQRMQENPTLEKPE
                     PQAKPQAVNAQAHRQSISISSQQLLQQPELLNSAMLTALVTNHTENVQFLLPIYQQLP
                     ASEQNSEVVQWAEAVNAKAKQNYSEAIKGYRQLLAKYPENQPIRMQLAVALFENRENE
                     AAEAQFYKLQAGKLPQEIANAVNSYLEAIGKQDQWSFQGGLTYLNDPNINNAPNAGTT
                     YGNWTAPKKESAQGVGFHFEADKKWSWGNGFFHEFRLNGNGKYYWNNKKYNEYSLRES
                     LGLGYQNAKQKITLLPFFEQMWYASGSSQTESTKRYSNAGGVNARWQYWITPQWQSAI
                     SYEYAEQRYTRRQHLDGNYHFIAPSLYFYPNSRQYWFVGANFNRTSPRDLDDSFIRRG
                     VSIGWGQEWKNGLSTQLSASYARKQYRAPMPIFQITQRNSEYGVQASIWHRALHFKGI
                     TPRLTWQYNKVKSNHSFYSYDKNRVYIEFSHTF"
     gene            complement(31870..34467)
                     /gene="mutS"
                     /locus_tag="PWY43_000184"
     CDS             complement(31870..34467)
                     /gene="mutS"
                     /locus_tag="PWY43_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2135104.1"
                     /translation="MTQDFSTHTPMMQQYLQLKAQNPDILLFYRMGDFYELFYDDAKR
                     AAALLDISLTKRGQSAGEPIPMAGVPYHAVEGYLAKLVALGESVAICEQIGDPATSKG
                     PVERKVVRIVTPGTVSDEALLPERQDNLVAAVYEEKGTFAIATLDMTSGRFLISELAS
                     KEALSAELQRVQPAEILYAEDFSAASILTNYKGLRRRPVWEFELVTAIQLLNRQFGTQ
                     TLAGFGVEKAVVALCAAGCVLHYAQETQRTALPHINAIHLAQHSDTILLDAATRRNLE
                     LTQNLAGGTENTLAAVLDKCVTPMGSRLLKRWIHQPIRDCGKLQARQKAITALIQYER
                     MSDLQPLLQQVGDMERILARVALRTARPRDLTRLRTALAQLPEIAKNSQNLTASIDQL
                     LAQLADFSPLHDLLERAIIENPPQLIRDGGVIAEGYNAELDEWRQLSDGATQYLENLE
                     IRERESTGIDSLKIGFNAVHGYYIQISQGQAHKAPIHYVRRQTLKNAERYIIPELKTY
                     EDKVLKAKGASLALEKQLYDELFDVLMPRLGELQLAAMMLSELDVLTNLAERADSLGY
                     VCPSFSPMRMVNIKGGRHPVVEQVLTEPFIANPVFLNAQRHLLIVTGPNMGGKSTYMR
                     QIALITLMAYIGSFVPAESAEIGPIDRIFTRIGASDDLASGRSTFMVEMTEMANILHQ
                     ATENSLVLIDEIGRGTSTYDGLSLAWACAEWLAKKTQSLTLFATHYFELTGLPNQLKG
                     VANVHLDAREHQDSIVFMHSVQEGAASKSYGLAVAALAGVPKQVIQLAKQRLAHLEEI
                     SHQTKQANEHPQADLLFSDGFDLQKNEAIQPLVVENEVEKALKALQPDELTPRQALDE
                     LYRLKKLLG"
     gene            34617..35180
                     /locus_tag="PWY43_000185"
     CDS             34617..35180
                     /locus_tag="PWY43_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5358 domain-containing protein"
                     /protein_id="HDL2135105.1"
                     /translation="MLRKIVLLSTGLLLAACSSNEPQQPEAFPGEFANADYVLSEENA
                     QKWVIASEQAKQCIYPNLTRIQQEHFSKEDSYIFSQYVFFYPLEEIIGEQYVKIIQND
                     EKSMGYAQYLFKKLKNNEAVEALPKKQCATLRTNAKNDLAVVKGQYQSGMIEETNSQE
                     GKSGDKVATNDNKFFFDIIKWGSMLLL"
     gene            35354..35983
                     /locus_tag="PWY43_000186"
     CDS             35354..35983
                     /locus_tag="PWY43_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL2135106.1"
                     /translation="MAVSATSFAQTTQPTAAEQMNAVKASVAEKATVAKEATKEKAAE
                     LKAGSAEKMATAKAKTESFKDSAKNKMDAVKATTAEKTDVIKAKAASASDTMKVKAQE
                     AKAATAEKAATAHETLNSAKTATAEKAAPMKAAATEKMSAVKKSQKVNINTANEEQLQ
                     SLTGIGEAKAKAIVEYRKKHGKIKNAAELANIPGIGDATIEKVAPYLTF"
     gene            36131..36760
                     /locus_tag="PWY43_000187"
     CDS             36131..36760
                     /locus_tag="PWY43_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2135107.1"
                     /translation="MALDFNSVGLDSVEAKGGYGIGLQIGQQLLGSGMDVNAEAVARG
                     IYDVLNNNEPAIDINEVTAALQELGQRAEAAQAEAFKAIDAENKAFLEENKKANGVIV
                     TDSGLQYEILTEGTGEKPTATSTVRVHYTGSLIDGTVFDSSVKRGQPAEFPVNGVIRG
                     WTEALQLMPVGSKWRLTIPQELAYGERGAGASIPPFATLVFEVELLDIL"
     gene            36826..37341
                     /locus_tag="PWY43_000188"
     CDS             36826..37341
                     /locus_tag="PWY43_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDL2135108.1"
                     /translation="MQKKSLISRLLKNIFLYGSLFIIISLVVDWYRSPSAPAQFEQTV
                     HYDIQHQPKVIAQLSHEKPMLLYFWGSWCHYCQVVSPNIQQLANDGTEVLGVALKSGD
                     ENDVQAYLNENGYTFATLNDPSGDFSRGWNIQATPTILIIKDGKIAHHTTGYTSYLSL
                     KFRLWLADVLY"
     gene            complement(37389..38443)
                     /locus_tag="PWY43_000189"
                     /pseudo
     CDS             complement(37389..38443)
                     /locus_tag="PWY43_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009500034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            38640..39998
                     /gene="mnmE"
                     /locus_tag="PWY43_000190"
     CDS             38640..39998
                     /gene="mnmE"
                     /locus_tag="PWY43_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL2135109.1"
                     /translation="MKDTIVAQATPIGRGGVGILRVSGPLAETVAQAVLGKTLPPRIA
                     NYLPFKDEDGTVLDQGIALFFKAPNSFTGEDVLELQGHGGQVILDLLLKRILQVKGLR
                     IARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINALVDS
                     VIYLRTYVEAAIDFPDEEIDFLADGKIEAKLNEIIAQLDGVRREAKQGSILREGMKVV
                     IAGRPNAGKSSLLNALAGREAAIVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAS
                     DEVERIGIQRAWEEIAQADHVLLMIDSTEQQADQFRQEWAEFLAKLPANMPVTVIRNK
                     VDLSGEQEGLIQVDDFTMIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQ
                     ALETAAEHLERGHIQLTQFLAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCI
                     GK"
     gene            39999..41264
                     /gene="tilS"
                     /locus_tag="PWY43_000191"
     CDS             39999..41264
                     /gene="tilS"
                     /locus_tag="PWY43_000191"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL2135110.1"
                     /translation="MTLFNRFQTQCATHLPNQTAFLIGLSGGVDSVVLLHLFARTSFH
                     VRAIYVHHGLSPNADDWAAFCEQYCKRLNIPFILQKVRVDSSNGVENGAREARYQAIQ
                     QQLKPNEVFATAHHLDDQAETFLLALKRGSGVKGLSAMQAVTFLQNFTVFRPLLTFTK
                     SDLMGYAVQHQLGWIEDESNADNRYDRNFLRNSILPLLNQRWQHFSQMVARSAQHCAE
                     QQALIEELLSDELKSRTGEKQQLNINGFGQFSLAKQQQLSRLWLEQNGVRMPSQAQLQ
                     AVISELIFAKADKNPQVKVGENIIRRYQQAIYVTEEIADIPPFEVVLPTETKVELLYQ
                     LGTIIRQQQEIICKKNGKNHRLQLPQELAQSTISIKIGQLGKVKCYGKAQREEMKKIW
                     QNHGIPVWERNRTPLIFWQDELVGLLPIL"
     gene            complement(41254..42147)
                     /gene="xerD"
                     /locus_tag="PWY43_000192"
     CDS             complement(41254..42147)
                     /gene="xerD"
                     /locus_tag="PWY43_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL2135111.1"
                     /translation="MAELDPIIEQFLDTLWQEHGLSENTVASYRSDLERFSDYFSEPK
                     AFLSLDHIDLQGFLGERLEQGYKATSSARMLSCLRKFFRFLCLEHYRQDDPTLTLSSP
                     KRGASLPKSLSEDQVMDLLDAPNTLDPIELRDKAMLELLYATGLRVTELVSLSMDNIS
                     LKQGGVRIIGKGDKERLVPLGEEANYWIQEFFQYGRPILLNNQQSDVVFPSRRGQQMT
                     RQTFWHRIKHYAVLAGIDADKLSPHVLRHAFATHLVNHGADLRVVQMLLGHSDLSTTQ
                     IYTQVAKARLKSLHQQFHPRG"
BASE COUNT        12586 a         9446 c         8486 g        11739 t
ORIGIN      
        1 ataatcgcca cattcagcaa caccggggaa aatgccatca ccccaaactt accgatggta
       61 tttaacaccg caccggataa cgccacaaag gtaataaacc acaaataagg gaaagtgatt
      121 ttgagcaaaa aagaggcttg ggtgaatttt tcggcatttg gcccgtcgta aagccaatct
      181 aaaaaccaac ctgtaccaaa aagagcggca accacaggcg aggcaatcat tgccacaagg
      241 gtaacaacag ttaccaaacc tcctaatgta cccgatactt tggcaataaa ttctctcgtt
      301 ttgtttggat cattatccgc attatattcc gccaacaccg gcacaaaggc tttagaaaat
      361 gccccttcgg caaacaaacg gcgaagaaaa ttgggaatac ggttggcaaa caggaaaata
      421 tcggcactca cgcctgtgcc taaaatggtt gccaccacaa tgtctcgaat caagccgagt
      481 atgcgagaaa ttaaggtcat aaagcttacg atcattcctg atcttaaaag ttttttgctc
      541 ataataaaga gtaatattag taaaattatt gaaaaatcag accgtttgta gtctgatcct
      601 aaaaatcgat cataatttta gcagtttgtt ataggaaaat cagaaaaaaa ctgttaaaat
      661 ctcgccctat cgttttctat cagaaaaacg gtgttatttt caagcacgtg tctcgttgaa
      721 aatgaattaa aaactatcga taggcaatat aaacaacgat tttgtttata tctaattttt
      781 acacagatat tttaggagtt tgaccttggc taatatcaag tcagcaaaaa aacgtgcggt
      841 tcaatctgaa aaacgccgcc aacacaacgc aagccaacgc tcaatgatgc gtacattcat
      901 caaaaaagta tatgcagcag taacagcagg cgataaagca gcagctcaaa cggctttcgt
      961 tgaaatgcaa aaagttgtag accgtatggc atctaaaaac ttaatccacg ctaacaaagc
     1021 ggctaaccac aaatctaaat tagcagcgca aatcaaaaaa ttagcgtaat atcgaacaga
     1081 tatgaaaaaa accgacttta tgtcggtttt tttcttttta accataacac aaagggctat
     1141 tctagatatt ctaaaatagc ccttgtttca aaatcagtta attatttttt caataaatca
     1201 cggatttcag ttaataattt ctcttccgct gttggctctg caggagcttc ttctacaact
     1261 ggagcttttt tcagtgagtt aatcactttc accataccga ataccgcaag tgcgataatt
     1321 aagaaatcaa atacgttttg gataaatgca ccgtatttta acatcactgc ttccgcacct
     1381 tcttttgccg gggcgatttc aatggctaaa tctttgaaat ccacgccacc gattaaccaa
     1441 ccgataggtg gcattacaac atcgctaact aatgaagaaa caattttacc gaatgcaccg
     1501 ccgataatca caccgacagc catatcaaca acattacctt ttaccgcaaa ttcacggaac
     1561 tcttttaaaa tactcatagg gggctttcct ttcattcaat gattaaacac ggtcttcgca
     1621 agaacgtggg ctggcgatta taatcaaaat ctttaaaatt tctacaacat ttaaataaat
     1681 ttacattttt ttgatttaga tcagtttgat tataactgat tggctaataa tttgccaaaa
     1741 ttctccacct gctgccaatc ggtgtactca tattctttgg aggtatcggt ttcgcctttg
     1801 gtaattttca taataaagcg aatcatcaat cggtcgaatg gcttatagcg taggtaaagt
     1861 aatgccccag caattacctc aatgtgggtt ggtttccatt tcactttagc taaaaattta
     1921 cgcacataag tattggtttc aggcgtgttt cgattatctt tgcgagcggt caaattcaca
     1981 ccgtaaaagg cagttttctt ggcgtttaat gccggataat gctgttccac aaactgataa
     2041 accaacgggt taaaatgccc atagcgaatt gaagcaccga tgacaatttg gtcggcattt
     2101 gcaatttttt cggcaaaatc gaccgcttga tcttttagcg aaatcagctc gacttcgtga
     2161 gtaatcacgt ccgccaatcg ttcggcaatt tttttggttt gaccatcggt cgtaaaatag
     2221 agaattaagg ttttcattta atctttccaa aaaacagggc ttaaaatcac cagcaaggta
     2281 aacacttcca gacgtccgca taccattgca aaagtcagca ctaacttggc actgtccggc
     2341 acttgggtga aattgctgct cacgctccct aatgcaggac ctagattatt tagtgaggca
     2401 attaccgcat ttagagcatc aaaggtttcc attccgcaag cgatggtagc aagccagcag
     2461 atcacgaaaa cgaaaaagta cgccgagaaa aacgcccaaa taccatcaac cacccgttcc
     2521 gataaaacgt ggttgcctaa tttaatcggt tgaatcacat tggggtgaat gtagcggtgg
     2581 atttcacgtt tactttgtaa ataaagcagc aacacccgaa tcactttcaa gccgccaccg
     2641 gttgagccgg cacaaccacc gataaatgaa gacagcacca acagcgtggg taaaaacgaa
     2701 ctccattggc taaaatcatt ggtagtaaag ccggccgtgg tggaaatgga caccgattga
     2761 aacaacgctt gctcaatcgt aacagaggta ttatcgaaat aaccgtaacc cactaacacc
     2821 aaggtacaaa ccaagaaaag ccctaactgc actatcaaga aaaaacgaaa ctcgtaatcg
     2881 ccccaataca agcggcttaa aataccccct tttttgcgtg attgtgcatc tcgtatgcga
     2941 tcaatcaccg caaaatgcaa tgcaaagttg caggcagaaa gtagcaggaa aaacacggta
     3001 atatagctaa ttgccgcatt attaaaatag ccgatgctgg catcgtgggt agaaaatccg
     3061 ccaatcgaaa ccgtggaaaa gctatgcgaa atggcatcaa acaccgtcat tccggcgagc
     3121 caaaatgcga ccgcacacag aatcgtcagg ctgagataaa tcagccacaa cgcttttgcc
     3181 gtttccgcaa tgcgaggtcg cattttctgc tctttgagcg gtccgggcat ttccgcacgg
     3241 taaagttgca ttccaccaat gcctaacagc ggaataatcg caaccgctaa cacaatgatc
     3301 cccattcccc ctagccattg caaaaattgg cggtagaata aaatcgcttt gggcaagcta
     3361 tctaagccgg tgatgacggt tgcacccgtt gtagttaagc cggaaaagga ttcaaaaatg
     3421 gattccgcca cgtttaaatc ggggttttcc aaaataataa aaggcacagc cccgagcaaa
     3481 cccagcacca cccaaaacag tactacaatc aaaaagccct cacgggaacg tagcttgttt
     3541 ttgttgtcac ggcacagcca ccaaagcagc gtaccgacca taaaattcag agcgaaggct
     3601 tctaaaaacg cccgcccacc gccatcgcca taaattaggg cgaccgttgc cggcacaagc
     3661 attgtgaatg aaaaacacat caccaaaatg ccaacaatac gaataatgga aaaaaattgc
     3721 atagactaga tttcttcttt tattaatgcg agcttgcccg aaaagcgttg ggttaattca
     3781 tctgcaaagg cctcaacctc gctcgggtta aatgccagca acaaatccac tttatcgcta
     3841 aaaagttgct cgacaatcac aatatctttc tcgctaatga gatgagaaat ggtattaaac
     3901 tgctcatatt cacattgcaa gcggtagttt tttcgcaaaa ctttgcgaat ccgctccaac
     3961 tgcaataacg cctgttgcac cccattgcca taggctttca ccaagccgcc ggtgccaagc
     4021 tggataccac cgtagtagcg aaccaccacc gctgtaattt cccccaagcc tgatccaagc
     4081 aaataattga gcattggctt acctgctgta ccggacggtt ctccatcatc cgaaaagccg
     4141 tattgttgcg aatcattcgg caagcctgcc accgttgccc aacaccaatg gcgtgcgtgc
     4201 ggatgctgaa ttttcagctc ctgccaaaat gccttggcct gctccatacc ctcagtatgg
     4261 cgaaggtagg tgataaatcg gcttttttta atctcttctt caaaaattgt atccgctttg
     4321 gggataaaaa attccattgt ttgattctct tatcttgaat gtggcaatga taccatattc
     4381 aagccacttt ttatttgtgc tgtttaatgg tttaataagt ctttcattga taacttgaac
     4441 gaataaaaaa tatgtcaaaa ctcaatatta tttttgccgg cacgcctgat tttgccgctc
     4501 aacatttaca agccttatta gattccgagc ataacgtgat tgcggtttat actcagccgg
     4561 ataaacctgc cgggcgaggc aaaaaactgc aagccagccc ggtaaaacag ctggccgaag
     4621 ctcacaacat tcctgtttac caacccaaat ccctgcgtaa ggaagaggct caagctgaac
     4681 tgaaagccct aaatgccgat gtgatggtgg tggtagctta cggcttaatt ctacctgaag
     4741 cagtattgaa tgctcctaaa tatggttgcc taaacgtaca cggttcgcta ctacctcgct
     4801 ggcgtggagc agctccgatt cagcgttcga tttgggcagg tgatacagaa accggcgtaa
     4861 ccattatgct aatggacgtt ggcttagaca ccggtgatat gttacataaa gtcacaaccc
     4921 cgattgagcc gaatgaaacc tccgcctcct tgtatgccaa actggcagaa cttgcccctc
     4981 tggcattgct tgaggtgtta aacggtttag ccaaccacac attcaaagcg gaaaaacaag
     5041 atgaagcact gtccaattac gccgagaaac tttccaaaga agaagctaaa ttggattggc
     5101 aactcccagc cgttcaactg gagcgcaata tccgagcttt taacccttgg ccgtttagtt
     5161 ttttaacctt aaccgttgat ggcgttgagc aaagtatcaa aattcaccaa gccacggtgt
     5221 tacctcacca agaaaaagca gccggcacgg taattaatgt ggataaaacc gggattcaaa
     5281 ttgccaccca acagggcgtg ctgaatatta cccaacttca accggcgagt aaaaaaccaa
     5341 tgtcggtgca agatttctta aacggtcgag cggattggtt cgtcagcggg acacaactaa
     5401 gttaaaacgg gaaatatatg caaacacagc cttcacttac aataaccaat gcccgatttt
     5461 cactggttaa acaacataaa ctcacgattg acgccttaca gatccaaccc aatgactttt
     5521 gggtggtcgt aggcggaaac ggttcaggca aaacagcttt ttcactcgct ttacaggata
     5581 aaatctcgct ctattccggc gactatggta attcgttcca acatattcag ctcctctctt
     5641 tcgagcaaca acaaaaaatg ttggatcaga ttttcaaaga tcggaacaac gatagtgtct
     5701 cgcccgatga tttcggctta accgcacgac aagtaatttt aaatggcagt ccaaaagagg
     5761 cattatgtga ggaatatgcc cacaaattgc atattcaaca tctgcttgaa cgcccgttta
     5821 tgcacctttc caccggtgaa agccgcaaag tgctattttg ccaaatgtta gtgagcgagc
     5881 cggatttatt cattttagac gaaccgtttg aagggctgga tcagcagtcg gtgcaatatt
     5941 ggcaacaact gattgccgaa ctacaaagca aaatggcgtt agtgctgatt gtaaatcgtt
     6001 ttaacgatat tcctgatgct gccaatcata ttgcgttatt agataattta tcattaattc
     6061 tgcaaggaga gcgagcagaa attgaacaac aagcggtcta ttctcagcta aaatttgcag
     6121 aacagaatgt gaacgttcct ttgcctgaaa gtgccgctcc actgaacagt ctgcccgaaa
     6181 acatcaatcc gtttgagctg gaaaaggtga ctatccaata cggcgaaaag aaaattttag
     6241 acaacctcag ttggacggtt gagccaaaac aacattggtg gattaaaggc ccgaatggag
     6301 ctgggaaatc gaccttgctt tcgattatca ccggcgatca cccacaatct tatgccaata
     6361 aagtccgact atttggcaaa caacggggaa gcggcgaaac catttgggat attaagaaaa
     6421 atatcggcta tgtcagcagc cagttgcata tggattatcg ggtaaattgc tccgcaatgg
     6481 aggtcattct ttctggcttt tttgacagca tcggcgtgta tcaacaagtg ccgaatgcct
     6541 tgcaattaaa agcaatggaa tggttggaac gcctcaattt aagcaatgtt gccaaaaagc
     6601 cgtttcgctc tctctcgtgg gggcaacaac gcttactttt gattacccga gcaatggtga
     6661 aacacccatc aattctgatt ctagacgaac cgttacaagg tttagacggc attaaccgca
     6721 aattagtcaa acagttcatt gaacaattag tgactaacag ccaaacgcaa ctgctttttg
     6781 tttcgcacca agacagcgat gctccaaatt gcattacgca tttatttgag tttgtaccga
     6841 aaggagaaac caacgagttt gggtttgagt atcaccaaac tgcacttgga gcataaatga
     6901 attttcttac ccattcgctg atttcacttg aaattgacac ccaaaccaag caacagaccc
     6961 tatatggcaa ctttgccggt gatttctata aaggtttaat tccgcatctc aatttagcgg
     7021 ataacttaaa aagcggtgtg gtgttacacc gctcgattga taagatttcg gaccgaccgg
     7081 acagcctcac aacccaatta ctccaaggca cattcgggcg ttataaaggc attgtgtcgg
     7141 atattatgat cgaccatttt ctcgctaaac atttccaacg gttatttgct aaagatttaa
     7201 atgaggtaca agcacagatc tttcagcaaa tcgaacaaca acgcacgatt tttcctgaac
     7261 aatttgtgcc gatgtttaac tggttacagt cacgaaaagc attaactcat tatgccgatt
     7321 tagatttctt agaacaggta ttcttgggaa tgtcatcaag agttaagaaa ggtgagattt
     7381 taagaaatgc tgtaaccgaa atttggcaga attatactct tttagaagaa gccgctattc
     7441 aggagttttt tactgtaaaa gacagggcta ctcaagaact tagttgaata ctttagtcgg
     7501 tatcttggat attcttgaat ttatcgatct tttctcttat cctgtctgat aataattctc
     7561 aaacattaca tttttatgca aacaacgctc tctccccaac aaatagctaa aaagcaccga
     7621 actattgaaa agaacgcagt tttttattac tctttttctt actcatttgt ttactcagcc
     7681 tcattttcga tattatgaca ggcccttccc tgcttccgtt taatgaagtt tggaaagcct
     7741 tattccaatt aggcgaaatt gaagaaacca ccaaagtgat tgtctatgag ctacgcttac
     7801 caatggcaat aatggcattg gtggttggag cagcacttgg tgttggcgga gctgaaattc
     7861 aaactttgct taataatcca atggcaagcc cttatacatt gggtttagca gctgcagccg
     7921 gctttggtgc ttcgattgtg attgcctttg gtggatttgg tttacccacc gaaatttctg
     7981 tacctgtcgg ggcttttgtg atgacactat tttcttccgc cattttattt gccttttcct
     8041 ctttaggacg ttttagctcg gcaatattag tgctggtagg aattgcctta ttgtttttat
     8101 ttcagtcttt actgtcattg gtgcaattta tttctgcacc tgaggtatcc caacaaattc
     8161 tgttttggct attcggtaat ttaactaaag ctacttggca gactatctca attgcctttg
     8221 gcgtaactac agtctgtatt ttattattat tcaaagatgc gtggaaatta actgccttgc
     8281 gtttgggcga agctcgagca ttgagcttag gcattaactt aacttggtta cgaatgaaaa
     8341 cattgctgat tgtgtctgta atgacagcca cctgcattag ctttgtaggc attatcggct
     8401 ttattggctt agttgccccg catattgccc gaatgttgct aggcgaagac caacgctttt
     8461 tcctgcccgg ttcaatgtta gtgggtgcgg catttttatc gatgtcatcc gtgctatcaa
     8521 aaagccttgt gccgggcgca ttatttccgg tcggtattat tacggcatta gtcggtgtgc
     8581 cgttcttttt ctggattgta ttggcgaaac gataaggatt ttctatgctc aacttaaacg
     8641 atgtaactat taagcgtgga aacttagtgg tggctgataa aatcaatcta cattttgaac
     8701 aaggcaagat ctatacttta cttggcccaa atggtgcggg taaatcttct ctccttaaaa
     8761 ctctatttgg ggaaattgct catcaaggca caattgattt tgacggtaat aaattaacct
     8821 caagccactt gcatagttgg cgtaaagaaa ttggctatat gccacaagac acacaggtag
     8881 aggcctcact cagtgcctta gaggttattt tattagggca acttgatagc ctgaatatgt
     8941 atattagcga tgaattactc aatgaagccg ttgatattat ggataaactc ggtatttcac
     9001 atctagctca tcaagatatt atgcggctaa gtggcgggca acggcaaatg gtaatgttcg
     9061 cacaagtatt gctacgtaaa ccacacattc tgctgctaga tgagcctgtc agtgcgttgg
     9121 atatgtatca tcaaatcaac ttacttgaat atgtgaatgc ctacactcga gcgaataacc
     9181 taattacaat tatggtgctg cacgatttaa gccttgcggc tcaagtgtga tgctattaag
     9241 cgaggggaaa attcacgcac aaggcagagc ttgtgatgtg ttggaatgcg atattattca
     9301 gagactctat aatgtcaata ttgagatttt atatgatgga aacggctcgc cgattatccg
     9361 cccgcttcgc aaatgtttaa atattgattg ctgtttgaac cgcaaatagt aaaagcgaat
     9421 acaagcggtc attttgcaaa ataaatctgc aaaatgatcg cttgttgttt ttatttcagt
     9481 tgcataaaga atgtgccttt tggcgtaatt ggtaagtagt tgttgtggaa atccaaatac
     9541 gttttttcag gatcgatatc tttaaacaat tcaggataaa gtgcttttgc cataaattga
     9601 gcactcgcta aatctgaaat tgagcgagaa gctgtatgat ataaaccata aacacgccct
     9661 gattttaccg caggtaactc tttccaacct gtacgctcaa taaagccttt taagcgtttt
     9721 tgagcatcgg cttcttcaat attaatcccc atcgacataa tctcagggtt agtgtcgttg
     9781 cctagttccg tacctgaaat cataatcaca tcaggtttag aaactaacac ctgctcaggg
     9841 ttaatctgcc cccagttttc tacaaacggg gcactaatgt tatcgcctcg caccgtttcg
     9901 gcaatcgctc cccacatatt tttaccaaag gtaaaactat gctctttcgg gcctttatta
     9961 ccaaattcag catagatttt cggcttactt acttttgcat tatcaataca gctctgaata
    10021 tcagcaatgc cctcttcata ctctttggca attttctccg ctctctcttc agtgcctgta
    10081 agctgaccga aaactctcac gctttttgta tggttttcaa cagtttgctt gttgaaatct
    10141 actactacaa caggaatgcc agcttgctca atgcgtggaa tttcactagc taacgcttga
    10201 tattgaattt caggtagcac taacacatct ggttttaatg cgatggtttt ttctgttgaa
    10261 aaagtaccac tattcacata gccaagatcg gcaatgtttt ttagatttgg cattttttct
    10321 gcaaataatg cccaactgcc tgggttgaac ttttcccaaa actctcgtga aatccctact
    10381 accttatcga aattttcaac accggttacc gcaatatagt cagggtaata gaaagtcagt
    10441 gcaacacgtt tagcaggcac atcaactttg acctcacgcc ctaatacatc agtgatggtt
    10501 ttaatttcgg cattggcagt tgcaccaaaa gcaagcaatg tggcagcaaa tacgctgttt
    10561 aatagtaatt ttttcattgg aactccaaaa agaaatggaa agttgaattg taactttcta
    10621 taacaaatag tcaatgaaaa taattcgcaa caacgaaatt atctatagaa gagtaaatta
    10681 gaagagatta caagcggttt aattttgcta aaactttgca aaccacaaaa acaaagggca
    10741 aataatattc gcccttatcg attttatttc accaattcgc ctttcgcttg taaatccgcg
    10801 tggtaagaag aacgcacgaa cggtccgcag gctgcgtgtt caaagcccat tttttcagct
    10861 tcctcacgga acatatcaaa ctctgccggt ggcacataac gttccacttt taagtggtgg
    10921 cggcttggtt gtaagtattg cccgatggtg agcatcgtca cgccgtggtc tcgtaaatct
    10981 tgcattactt ccaagatttc ttcgttggtt tcacctaagc ctaccattaa gccggatttg
    11041 gtcggaatat gcgggaactc ttgcttgaag attttcaata attccaacga ccatttgtaa
    11101 tccgctcccg gacgaacttc acgatagaga cgtggcacat tttctaggtt gtggttaaag
    11161 acatcaggtg gattttcttt gagaattgcc accgcttgct ccacacgtcc acggaaatca
    11221 ggcactaaaa tttcaacctt acaatgcgga tttaaggctt tgatttcacg cacggtcgca
    11281 gagaaatgag ccgcaccacg gtcgggcaaa tcatcacgat ccactgaggt aatcaccaca
    11341 tatttgagtt tcatatcttg caccgtttct gccactttgc gaggttcgtc cgggtcaagc
    11401 ggaagcggtt tgccgtgtgc cacatcacag aacgggcaac gacgggtaca gattgccccc
    11461 ataatcataa aggttgcagt accgtggtta aaacattcgt gtaaattcgg gcaagaggct
    11521 tcctcgcata ccgagtgcaa gccgtggcga cgcataccgt ttttaatgct atcaattttt
    11581 gccgaatttg ccggcagttt gattttcatc cactccggtt tttttaagag ttcttgatcc
    11641 gggtcgatat tacgcacctg aatcacagag gttttggcgg catcacgata tttcacccct
    11701 ttttccattt tgaaagggct catttttttc actgtagcag ttgccgtcgt ttgagctgtc
    11761 gatgtagcca ttactgtgtt ttatcctaat tgttaataat ttgttggtta ttatagccta
    11821 aaattgtgct gaaatgagcc actaattttg gagcaacctc ttcgcaacgc aaatcagggc
    11881 gatgaaccag ttcacttaat tggcacattt ccagccctgc ataaccgcac ggattgatgt
    11941 tttgaaaagg cgttaagtcc atattgatat ttaatgccaa accgtggaaa gagcaacctt
    12001 ttcgaattcg taaaccaagt gagcagattt tcttgccgtc cacatacacc ccgggtgcat
    12061 cggcttttgg ataactttct atgccatatt ctgccaacgt ttgtaccacg gtttgctcaa
    12121 gggcagtcac taaatctcga accgacactt ctttacccaa ggatttcagc cgtttgatat
    12181 caatcagcac atacataatt tgctgcccct cgccgtggta agtaatttga ccgccacggt
    12241 cgctttgcac caccggaata ttggttggat ttaacaaatg ctccggtttg cctgccgatc
    12301 cttgcgtaaa cacagccggg tgctgaacca gccagatctc atcatcggta ttctcatcac
    12361 ggctatctgt gaaagattgc atttggtgcc aaatttcttc ataaggctga atgcctagtt
    12421 ggcgaattat taatttcatt ttctattttc caacatcgct ttattgaaaa attgagggcg
    12481 aatgtcattc gcccctcgca ttataatacc attcttacgc cattaatttt ggcaagttcc
    12541 gtgtagagag tttctacttg ctcaatattt tccgccacaa tatcaatcga aacggaatta
    12601 taggtacctt ttgagctttt ttgctgacgt ggattgtaat cgcctttggc atatttttgc
    12661 gtgaccacta ccacatcatc aaccaaatct tctcggtttg ctcccaccac tttgaacgtg
    12721 aaatgacacg gaaactctag cagatctttt aatttggctt gaggtaaatc ttgtaagttt
    12781 atcgctttgg gttgagacat ttttgatcct tattctattc tttcttcggg gggcgaaaaa
    12841 tttttcgcca ctacactttt tatacattca tatctaaaac ggtaagcggt gaaattttgc
    12901 aaattttttg caaaatcaca ccgcttgcat tgctccctag atggggataa aaaggcaaat
    12961 aacaagatta atcgaacaag ctcttcacag taagggcaat ccaatcccac gctttgccga
    13021 aaataccggc ttccggtaca tcttgtaaga cttgtaagtt agtgcttgca acatctttgc
    13081 cgtcaagttg gtagatcatt ttacccacta cctgcccttt tgctaaagga gcttcgaggt
    13141 ttttcttctc taactcataa cgggctttta aatctgccga gcgacctttc gggatagtca
    13201 caaagctatc ttgcaccgag cctaattgta ctttattgac ttcaccgtaa tagacacttt
    13261 gctctgccac cgattgagct gccggaatag atttaatggt ttcaaagttc gcaaagcccc
    13321 attgcagtaa tttcttactt tccacttcac gccctttata agttggcacg cccatcacta
    13381 cagaaattaa acgtgtattc gggttaattg ctgatgcgac taagttatag ccggctttat
    13441 cagtatgccc tgttttcata ccatcaactt gaatggtttt atcccacaat aatccgttgc
    13501 ggtttggttg tggttttttg atgttatatt ggaactcttt ttccgcataa attttatact
    13561 cttccggctg atcacgaata atatgcgaac caataatcgc catatcacga gcagaagaat
    13621 attgctcagg gtcatctaaa ccgtgaacag tggtaaagtg agtattcttt aagccaaact
    13681 gttgggcata tttgttcatt tcattaatga acgccgcttg tgagcctgaa acatgttcag
    13741 caagcgccac agaggcatca ttacccgaga cgataatcag ccctttcatc aactcaccta
    13801 ctgaaacttt gctgtttaaa tctaaaaaca ttaatgaaga gccggggaat ttttgcgccc
    13861 acgaattttc ggtgactatc accatatcac tgttttgcac ttttcccgat tttagggctt
    13921 caccaactac gtagctagtc atcattttgg tcaatgatgc aggatattga cgttgatccg
    13981 gatttaggct cactaaaact tcaccggagt tgtaatccat caaaatatag gtttgtgcgt
    14041 tgatctgggg cgccgataaa ttgaagttag tgtttaaatc cgcataggca atgcttgaaa
    14101 ccgcaaaacc taccacgctc atcgctttca ctaatgtttt tttcatattc cctcttaaat
    14161 taattcatta cataaaactg attagtcgat cccaattttg acttatcaac aatcgcaatt
    14221 ttcactcttg ccgtccccga atggtgcata ccgatttttt tcgctgcggc tgatgataaa
    14281 tccaagaccc gtgcgtgagc ataaggtccg cgatcattta cacgcaccac cacagaacga
    14341 ccatttcgta aattcgtcac ttcaattaaa gtaccaaatg gtaaagttcg atgtgcagcc
    14401 gtcatttttg cgttattgaa agtctcccca ttagcggttc ggcgaccatt aaagtaattt
    14461 gcataatagc tggcaacacc agtctggaaa tgttttttga ccagtggctt atgctgcttt
    14521 tttaccaatt ttttcttagc ggttaccgtt ttcttgacaa ccttgcgagc cagctttttt
    14581 gtagatttaa tcggcttagt tgtaacagta gaaattgttt tcttttttgt agctttagcc
    14641 actacttctt ttttcttaac tttagaaata cctacttttt ttattgcttt agaagttgct
    14701 tgcttcttgg ctaaaatact cgatttagct tttgtattct ttgtcgcagc caaagaattt
    14761 aatgaactaa atgtcagaaa tccagctaac aacagggtta caattttacc taatctcata
    14821 taagtttttt cctctatgtg gatgtcgtta ttgtttgtaa cgatgatcgt ttcactgctt
    14881 tttgataccg caaaaaagca aaagtttaat ttatttcagt ttatcataag gattattact
    14941 acctacacgc ttacggtgaa cataggttga catcatcaaa ccaaatgccg ccatcagcgt
    15001 cacataggag gttccgccgt aactgaaaag cggtaatggt acaccgacta cgggtaaaat
    15061 accactcacc ataccaatat ttacaaaaac gtaaacaaaa aatagaaggg ctgtaccacc
    15121 tgataatata cgcccgaaag cagaatccga ttttgtgcca atcactaaac ctcgggcaat
    15181 aataaacaaa taaatcgcta ataaaatcag cacacctatc ataccatgct cttcgcttaa
    15241 cactgcaaaa ataaaatcgg tatgcggttc aggtaaaaat tcaagctggg attgcgttcc
    15301 ttccatccag ccttttccat taatgccacc agagcctatc gcaattttgg attgaataat
    15361 atgatagccg gcacccaacg ggtctttatt aggatcaatt aaggtcatca cccgggtttt
    15421 ttgataatcg tgcattaaga aaaaccacat tatcgggata aaaccggcta aaaacaccac
    15481 acctgcgcca attaatttcc aacttagccc tgccaaaaat aaaacaaaaa taccggcaga
    15541 acacactaaa atagatgttc ccaaatcggg ctgaattgcc accaataagg ttggcacaat
    15601 aataatggct aaggcgataa aagtatcttt aaaactcgga ggaagcggcc gcttacctaa
    15661 ataagtggct accatcagtg gcacagcgag cttggcaatc tcagaaggct gaaagcgaac
    15721 aaatcctaaa ttcaaccaac gttgcgctcc tttactggtt tcgccgacca aatccaccaa
    15781 aatcaataat atcagacaga aaatatacag ataaggcgaa actttttgat aaaagcgagg
    15841 aggtaccatt gccattatca acattagccc aagccctagc gaaacttgaa tcagtctact
    15901 ggtaaacatc cgctcatttg caccgcttgc gctatatagt actaataaac cataaccggt
    15961 aatagctaat aatcctgata ataaaaggag atcgagtgaa aaaattttcc agaaaagttt
    16021 tttaaagcca ctattcatga tgttcctctg tgacaagcgg tttattttgc tcagaatttt
    16081 gcgaatccgg ctgttcacca atgccttttc tcaaagcata atccataatc tgacgagcaa
    16141 caggagccgc ggcactacca ccaccgcctg cattttctaa aattaatgcc accactattt
    16201 taggttgttc ataaggagca tagccgataa accaagcgtg gtcgtgcaac tcttttttaa
    16261 tcgcattttt atcgtaattt ttaccgttta agttaaacac ctgagccgta ccggttttcc
    16321 ctgctgaatg ataaaatgca ccggcaaaca cttttcgtgc cgtaccgttt gcggcgtggt
    16381 ttacgttata cattccctgt tttgctaaat tccaatagac atccggcacg cctttaatat
    16441 cttcatacaa caatgggtct tgatacggcg taaccgaatt actgctttgc acttcaagca
    16501 ttaaatgcgg tgtattcacc cgcccgttgt tgattaacac cgccgtggct ttcgccaact
    16561 gtaacggggt cgaaatccag tagccctgcc caatcccaac cggaatagta tcgccctgca
    16621 cccaaggttt tttatagcgt ttttgtttcc attcacgggt tggcattacc cctgcggatt
    16681 cctcaaaaat atcgataccg gttttcactc cgaaaccgaa ttttttcatc caatccgaca
    16741 ttttatcaat ccccatttta taggctaatt gatagaaaaa ggtatcagac gattcggtaa
    16801 ttgctttatt cacattagtt gcaccgtgtc cgctacgttt ccaatctcga taacgttgtg
    16861 tagtgccggg caaaatccaa tagccggggt ctgaaatggt ggtagaaggg gtaatcattc
    16921 cctcttccaa acctgccacc gccataaatg gcttaatagt tgaagccggt ggataagtgc
    16981 cttgtgtagt acggctataa agtggtcggg ttggttcgtc caataatgct ttataatcct
    17041 gccctgaaat accgcccaca aataaattat tgtcataact tgggtttgag accatcgcta
    17101 aaatactgct atcacgaggg tcaagcacca ccaccgcccc tttatgctta gcaattaacg
    17161 acatcacata ttgctgtaat tccacatcaa tactcaagcg aatactgcta ccggcaaccc
    17221 ctgcttggtc acgcaattta cggattactt tccctcggtt attaatttct acctcttcaa
    17281 aaccggtgac accgtggagc tgatcttcat agaatttttc aatccccaat ttccccatat
    17341 cgtgcgaacc ggcataattg ccgaatttcc cggcttcttg cagctttttc ttatcgtttt
    17401 cgttaatttt cgccacataa ccgataatat gggttaaaag ctcgccgtaa ggataattcc
    17461 gcttataata cgcctgcaca tctaaacttg gaaaacggta ttggttcacc gcaaaacgag
    17521 ccatctgagc ctcattcaaa ttgcctttga gcataattgg ggtatagcga gaactgcgtt
    17581 ttttctcttt atgataattc tcgatatctt cgtccgtcag cccaacaata tccttcaact
    17641 cggttaaagt ctgctcaatg ttttccgtct tttcaggcac aataaacagc ccgaaatagg
    17701 ttaaattttc cgccaatact ttaccgttac ggtcataaat taagcctcga gccggtggca
    17761 caggcaacaa tttaatgcgg ttaccgtttg aacgggtttg gtagtcctca taattcacca
    17821 cttgcaagtg atacaagttg gttaataaaa caccacttaa cgccaacacc cccagaaacg
    17881 caactaaggc acggcgggca tacagattgc tttctgcctg cccgtctcgc accttttctc
    17941 gctggtttga agctgtgaat tttgctaatt taccaaacat ataacactat ctaaaatgac
    18001 tcactactct cgatgataag ggtgattaat ggtaaccgac cacgcccgat acaaactttc
    18061 cgccaccacc acccgcacca gtgggtgagg taaggttaaa ggcgacaacg accaactctg
    18121 ctcggcagcg gctttgcatt ccggtgataa tccttccggg ccgccaatca ataacgctac
    18181 atctcgccca tcgtttttcc agccctctaa ctgagtggct aactgctcgg ttgtccacgc
    18241 tttgcccgga atatccaaag tcacgatttt tgccttgccc gctgccgcca acattgcttt
    18301 gccttcaagc tcaagaatgc gtttagtatc agcattcttg ccccgttttc ctgccggaat
    18361 ttccaccaac tcaaacggca tctctttcgg aaaacggcgt tgatactctt caaaccctgt
    18421 cgttacccac gccggcattt tagtgccaac cgcaattaat tgaattttca tctctctttc
    18481 acttacaagc ggtttaattt gcaaaaaatg ttgcaaaaat caccgcttgt cgttccttat
    18541 tgaactgcta cgcccaaagt ttctctaatt ggtacatctc acggctatcg gattgcaaca
    18601 agtgaaccat tgccttccca aaatcgacca cgatccaatc ggcaactgct ttaccttcat
    18661 ctccaaatac ctcgaaccca gcctgttttg cttccgtaat taaacgctct gccgttgaag
    18721 caacgtgacg gctcgatgta ccggtggcta aaatcatcgt atcagtaatg ctagatttcc
    18781 ctttcacatc aatggctaaa atatcttgtg cctttaaatc atctaaggtt ttggttaaaa
    18841 attcgactaa attctgttcc acgtttctct ctctcatctt ataaaaacgt agaattttaa
    18901 catagattga acggataaaa gaaagaaagc ggtcattttt gcaagatttt ttgcaaaaat
    18961 gaccgcttgt tacttactct aaaatgccta actgcttcca atactatggc atatttgctt
    19021 ttgttttacc tattttttga gtcgtagaat agggagtcga taaataatca atgctatcaa
    19081 aatcaggctg caacctagcc attttgtcgc actaagttgt tcccccaata acatcacgct
    19141 caataacaat gtccaaaccg gctcaagtaa cataatgagt gcagaagtac caatatggca
    19201 atatttttgc cccagtgttt gcattaaaaa acgtaaattt gtcgcaatca atacacttgc
    19261 ggcaaaccag agccacactt cggtgggaat cgggaaatgc caactttcca taaaatagga
    19321 atacaagcta cagaccgcac cgactatcgc caattggata gtaattaatg gaaacaatga
    19381 gacttgtttg gcgtattgat tatttaacac aaaatataac gccccaaaca ctgatgacac
    19441 cagaaaaatc gtactgccta aggaaaaatg aaagatgcct ttctccgcag aaagaaagta
    19501 aagcccactt aatgcaatgg gtaaggctaa ccaaaaaatc ttcggtggcg tatgtttgaa
    19561 tatcaaccaa gagagcaaag gagcaatcag catcgcaagg ctcaccaaaa aagccccttc
    19621 accaaacact gtactaaaag ccagccccaa tacccaaaaa atagtataaa ttccgtaagt
    19681 aagaccggta atcaccgaat atttaacttg aaccttatct aaacgataca gctctcggta
    19741 agaaaaaggc agaagaatca aagaggctaa tataaaacgc cctcccataa agccaatagg
    19801 cggtaatcca actaaagcgt gtttagagaa aaaccacccc gctgcagcaa taaaggtaac
    19861 gagaaaaagg attatttcgc cgaaatattt tttgaacata tcaaactata aaatgccctc
    19921 tacagtagag ggcattataa taaataaggc taatcgttaa aatagacttt cttaagtgcc
    19981 aactccaatc cacggaactc cgctaatcct cttaaacgac caatcgcaga atagcctggg
    20041 ttggtttttt tcttcagatc atcgagcatt tgatggccgt gatccggacg cattggaatt
    20101 aagcggtgat cgccatttgc cttgcggcga tactcttcgg taagtaatgc tttcaccacg
    20161 ccaaacatat cgacatcacc ggcaagatga tccgcttcgt ggaaactttg tggattttct
    20221 tcacgttggg tagctcgtaa gtgagcaaaa taaatgcgat ctgcaaactg ctctgccatt
    20281 ttgaccaaat cattctcggc taatacgccg tatgaaccgg tacagaacgt gaagccattg
    20341 gctgcaagcg gttgagtttc cacaaaccat tgcatatctt ctatggttga aacaatgcgt
    20401 ggcaagccta aaatcgggcg aggcggatcg tcagggtgaa tcgccatacg aatgcccacc
    20461 tcttgtgcaa ccggcacaat ctcatttaag aaataggcta aatgggtacg gaatttttca
    20521 gccgagagcc cttgatagcg gtcaagctgt gcttggaatt cttcgagcgt atagccttcc
    20581 tcagaccccg gcaaaccggc aatgatattt ttggttaatt ttgcaatatc agcgtcactc
    20641 atctgctcaa aataggcttt ggcttcggct tgttctgctg atgagtaagt ggcttccgct
    20701 cccgggcgtt ttaagatata taactcaaac gccgcaaacg ccacttggtc aaaacgcaat
    20761 gcttttgagc cgtcaggcat ttcatactcc agatcggtac gagtccaatc taacaccggc
    20821 ataaagttat agcacaccgt atcaatgccg cagcttgcca aattacgcaa ggtttgctta
    20881 tagttatcaa tccattgctg atagttgccg gtttgagtct tgatttcttc gtgaacaggc
    20941 acactttcta ccaccgacca agccagccct ttttcagcta ataatgcctt acgtttatta
    21001 atttcctcaa ccgaccacac ctgcccatta ggaatatggt gcaacgctgt cacaatcccc
    21061 gttgcccctg cttgtcttac atcatctaaa gatactggat cgtttggtcc ataccaacgc
    21121 catgcttgtt ccatagtaaa ctcctcatct aattaaaaat taacagccaa agggctaaca
    21181 ttaaacagat aaccatcaaa attcggatcg tctaaatccg ataattcaaa cagcttttgt
    21241 tttacatttt ccaaatgctg ccacatcatt tttcgagcca atacaggatc ttttcgctgt
    21301 attgccgcaa taatattttc gtgatctttt aaccataacg tgcggtaact ttgatcggga
    21361 atatgtgaat gtaacccttt ccacatcgga ctattggttc gatactccca taaatctttt
    21421 tgcatacgga taatcacttc attttgcgtt atttcagcaa tcgctttatg aaaatcttca
    21481 tctgcaacat aatcttcatc accgtgttct aaagtcctgc gttcacgctc taaaatctct
    21541 tttaagtgta aaacatctgc ccgagtggct tgaatagcag caaactcggc aatactactt
    21601 tctaatagct gccttgcttg caacagttca aaaggaccaa catcaggcaa ctcttctgtt
    21661 tcctcagcat ttgatttggg taaattaatc acataaacac cggagccttt acgcacttca
    21721 attaaatttt ccagctcaag cataatcagt gcttcacgaa taacggtacg gctgacatta
    21781 aagtaattag ataaatcacg ctccggcggt aatttatccc ctactttgta tgcaccactt
    21841 aataattctg cttttaattc ttgcccgatt tttttataag agcgtagatc ttccgctgcc
    21901 attattttgt ttctctatag tattttaaaa aggtttacca aaatagcaca ttacatacta
    21961 aaagaccaag aaacaacagt aaattaaagc atattttagt taaatttaac aagaggtaaa
    22021 ccagtgtgtc gtaattttgt gatctatatc acaaaattac taacaattgg tttaccagtt
    22081 gtaaaagtgg tagtatattt gccatacttt aatcacccct gataggagat aagcaatatg
    22141 aatgtttcaa aaaaccataa tttcaaagat aaagttgtcg ttattacggg agctggaggt
    22201 gtgctctgtg cattttttgc acaagaaata gcaaaaacgg gtgctaaagt agctctatta
    22261 gatattaacc ttgaatcagc agaaaaatat gcacaggcta ttgtgcaaaa tggaggagca
    22321 gccaaagctt ataaaaccaa tgtgcttgat ttagaaagta ttcagcaaac tcgcaatgcc
    22381 atcaacaaag actttggtac ttgtgacaca ttaattaatg gtgcaggagg taatagtcct
    22441 aaagccacca ccgataatga atttcatgaa tttaacttaa ctgatagtac aaaatcattc
    22501 tttgaattag ataaaagtgg cattgaattc gtatttaatc tcaactattt aggtacattg
    22561 ctacctactc aggtatttgc aaaagatatg attggtaaaa aaggagccag tatcatcaat
    22621 gtatcaagca tgaatgccta cacccctctt actaaaattc ctgcttactc tggagctaaa
    22681 gcagccatca gtaattttac tcagtggctt gccgtttatt tctctagggt aggaatccgc
    22741 tgtaatgcaa ttgcccctgg ctttctagtt agcaaccaaa atagagggtt acttttcgat
    22801 aatgaaggaa accctactcc tagagcgaat aaaattttaa ctaatacccc aatgggacgt
    22861 tttggtgaag cgaatgaatt gattggggga attttattct taatggatga aaattattcc
    22921 agcttcataa atggtgtggt attaccgatt gatggaggtt tctccgctta tagtggagta
    22981 taggagaatc tcatgacaat atttacacct aagcacaact ttattgtatg cattgattcg
    23041 gatggatgtg caatggatac aatgaacatt aagcatgagg agtgttttgg cccctctgct
    23101 gttaaagttt ttgacattac cgatagagat cgttttattg aaatttggga tagaatcaat
    23161 ctgtactctc aaacacgcgg catcaaccga tttaaagggt tggttatttc tctggaagag
    23221 tatgggtatg acaaaaattt cagccaatta aaacattggg taaatacaac ttctgaatta
    23281 tctaaccgct cgcttattca agctatacaa gagcaccctt ctcatgatct tcaacttgca
    23341 ttgatgtggt cagaaaaagt caatattggg attaaaactc tacatacaca agatagacca
    23401 tttcgtttag taaaacgctc tcttgctatt attaaagaat ttgctgatat cgccgtagta
    23461 agttctgcaa ataatgaagc aattattgat gagtggacac ggcatggttt actgcctttt
    23521 gttgatcttg tttatgggca agatgaagga acaaaaactc actgtttaaa ccaattatca
    23581 caatatggat atcagccaaa tcatatttta atggttggag attctcctgg ggatttaaat
    23641 agtgccaatg aagctggggt caatttcttc cctatattat gtggagatga agataactcc
    23701 tggaatcagt tggtattaga gacattagga aaattggttt accaaaaatt cactcttcaa
    23761 tatcaatctc aattaattgc aaaattcaat gcgaatctaa ccgcttgatc ccgtatttca
    23821 cggttttgaa gcgtaaatag gagaaattat gtctgttgat ttaacaaaaa aaccttatta
    23881 cttaaaagat gaagacattc aatgggtaaa cagcactatc gctaatatga ctctagaaga
    23941 aaaaatagga caactttttg tgaatatggg atcgagtaga acagaggaat atctcactga
    24001 tattttaaac cgctaccata ttggtgcagt acgttataac ccaggtccgg ctgaagaagt
    24061 ttataaccaa aactatattt tacaaaccaa aagcaaaatc cctctattaa ttgcggctaa
    24121 cactgaagct ggaggtaacg gtgcttgtag tgatggaacg gaaattggtt tacaggtcaa
    24181 aattggtgca acaggtgatg ccaaatatgc ttatgaaatg gggcgtgtag caggtattga
    24241 ggcagccgct attggctgta actggagttt tgcacctatc gttgatatta gctataactg
    24301 gagaaatcca attatttcta accgggtttt tggctctaat ccagataaag tactggaaat
    24361 gtccttagct tatatgaaag gcattcaaga aagcggaatt gcgccaacag ccaaacattt
    24421 ccctggagat ggggtcgatg aaagagatca acacctttct tttagcgtaa acagcttcag
    24481 ttgtgaagag tgagataact cttttggaaa agtctatcaa ggtctaattg atgccggttt
    24541 accctcaata atggctggtc atatccatct accagcttac gagcaacatt ttaaccctaa
    24601 tttatcgtat gaagactgtt tacccgcaac actttcaaag ccaattttaa ctgatttatt
    24661 acgaggtaaa ttaggtttta acggggttgt tgtaacagat gcaagtcata tggttgcaat
    24721 gacatccgct atgaaacgca gtgaaatgct gccgaaagcg attgcagctg gttgcgattt
    24781 attccttttc tttaatgatc ctgatgaaga ttttgattat atgatggccg gctataaaaa
    24841 cggcattatt acagaagaac gtctaaacga tgctcttacc cgtattctag gattaaaagc
    24901 aaaattagga ttacataatc gcccgaaaga aacattactt gaacctaaag aacaagcact
    24961 ggcaaaaata ggtttaccag aacataaagt gatcttccgt gatgtcgcag ataaagcaat
    25021 cacattagtg aagaataaac aagatatttt acctattaat gttgagcgtt ttccaagagt
    25081 cttacttgtg aatgtcaaag gcacagatgg tggattcggt aaaatggttg caggtagcca
    25141 acgtagcgca acagaaatcc taaaagaaaa actggcagaa aaagggttta cggtctctat
    25201 ttatacatca cctatggata atatcctcaa aatgtcagat gaagagcaag tgaataccat
    25261 ccgtaatgaa tactcgcaaa aacgcccaat tacaacatta accgatcact atgatctcat
    25321 tatcaatgtt gcaaatgttc aaatgagtac tgttcagcgt attgtatggc aagctaccaa
    25381 aggaacacct gatattccat tttatgtgca tgaagtacct acaattttcg tttccgtaca
    25441 atgcccattc catttagttg atgttccaca agtaaaaact tacatcaatg cttatgatgg
    25501 taaagagcca acaatagaac tgctagtcga aaaattaatg ggtaattctg agtttaaagg
    25561 cgttagccca gttgatgcat tctgtggtta taaagatacc catatttaat ccattattcc
    25621 taataaggag ttcttcatga gttcagtaca aagacctttt ggtctaaagg ataaacttgc
    25681 ttatatggca ggagatattg ccaatgacct cagctttatg atgtctgcat tcttcttaat
    25741 gctgttttat accaatgtat tacaaatcga aggctatgtt atcggtattt tatttttagt
    25801 ctctcgtatc atcgatgctt ttactgatgt tggtatgggg cgattggttg atacaattaa
    25861 gcctttcaaa gaagggcggt tcagagggct gttacgcaga acatcgccat ttgtttgtat
    25921 ctttggcttt ttaatgtttt tacacgtcgt taaagattgg gcatatgaag caaaacttat
    25981 ctatgtatgt gtgacctaca ttatttgggg aagctttgca tatacagcag taaatattcc
    26041 ttatggctca atggccactg ttatcagcaa caaaccagaa gatcgcgcat cactttccgt
    26101 atttcgtgca ataggtgcta acattgccct actatttatc tcattgtgta tcccattggt
    26161 aatttaccaa gaagttgatg gtaaacaggt cattcagcct gaaatgttta ctattgtgat
    26221 gggagtgttt atggtgatcg catttctcct ctatcaatac tgctggctca atacctgtga
    26281 acgggtgcag ttacctgaaa aggaaggaag acgccacaac aatgctgatt gtttatccga
    26341 tgtaaagagt atctttacca gcctatttag taatagaccg ttaatgatct tcattttagt
    26401 tgcgattatt ctgttattgg caaatctact gatcggcaca atgaatccct atctctacgt
    26461 tgattacttt aataacaaac tcggattatc tatcggagga atgctgcctg tgatcgcctc
    26521 atttgtagta gccccctttg ctcaacgtat tgtcaaacgc tttggcaaaa aagagtctgc
    26581 cgcaatcgcg ctactcttca ccggtgtagt ttatctcgct ctctttttcc taaaattgga
    26641 aaatgtttgg atctatttag ctaccgctct gattgcattg ctgggtttaa actactttat
    26701 ggttgtgatc tgggctttca ttacggatat tattgatgcg caatatctta aaacaaataa
    26761 acgagaagac ggtacgattt atgcggttta ctcgtttgca cgcaaaatcg ggcaagcgct
    26821 tgctggtggt ctcggtggtt tcgcattaag ttatgtcggc tatgtcgcca aagcaccaag
    26881 ccaaagtcag gagactttaa atgcggtcta taatgttgcc acactagtgc cggcaatctc
    26941 ttgtattatt attttcctgc tgctgaaatt tgtttatcca ctctccaaag aagtggtcga
    27001 taacaatgtc gcagaattag aaaaacgtca ccattaacct attggggtag gcaataacac
    27061 ctacccctac tttgttacca atcaatctcg agctaaatat gaaacaattt ttatgtgaag
    27121 actttttact ctcaactcca acggcacaaa agctctatca cgactatgcc aaagatcagc
    27181 cgatttttga ctaccactgc catttaaacc caaaagaaat tgcggaaaat cgccaattta
    27241 atgatttagc cgaaatctgg ctggaaggcg atcactacaa atggcgggca atgcgttctg
    27301 ccggtgtgga agagcgttta attacaggcg atgctgataa atacagtaaa tacttagcct
    27361 ttgcacaaac tgtgccaaaa tgtatcggca acccgattta ccattggacg catttagaac
    27421 ttcgccgtcc gtttgggatt actgacacgc tgttttcgcc acaaactgca gaaaaaattt
    27481 ggcatcaagg caaagcatta ctgcaacagc ctgaattttc cgcccgtggc attatgcaac
    27541 agatgaatgt gaaattagtc ggaactactg atgacccgat tgattctttg gaataccaca
    27601 aagcgattgc ggaagatagc acctttgaca ttgaggttgt gccaagtttc cgacctgacc
    27661 gagcatttaa aatcgaactg ccgctgttta atgactacat tgagcagctc ggtaacgttg
    27721 cggatattga aatcaacagc ttcgatcaat taaagcaagc actactaaaa cggattgaac
    27781 atttcgatca gcaggggtgt aaatccgccg atcacggcat tgaaatcgta cgttttgccg
    27841 ctattcctga tgaaacaaaa cttacccata ttttgcaaaa aagattgcaa aatcaaccgc
    27901 ttgcagaaga ggaaatcgca caattcagca cggcattatt agtctggtta ggcacggaat
    27961 accacaaacg caactgggtg atgcaaatgc acattggggc aatccgcaat aataacagcc
    28021 gtatgtttaa gttactcggg gcagacagcg gatttgactc cattggcgac cgcacctttg
    28081 ccgagccact ctcccgttta ttagacagta tggatcaaac tgatcaactt ccgaaaacca
    28141 ttttatattg cttaaatccg cgtgataacg aaatgctcgg tagtatgatc ggcaatttcc
    28201 aaaccggcgg cattgctggc aaaatccaat tgggttcagg ctggtggttc aatgatcaga
    28261 aagatggaat ggaacgccaa ctgcaacagc tttcacaatt aggtttgctt agccaatttg
    28321 tcggtatgct aaccgattcc cgtagtttct tatcttacac ccgccacgaa tacttccgcc
    28381 gtattctttg cgaaatgatt ggtggctggg ttgaacgggg tgaagcaccg aacgatctga
    28441 atttacttgg caatatggta aaaggtattt gctacggtaa tgcaaaacgt tatttcaaat
    28501 aggagataac aatgaaaaaa atcgctctaa ttggcgaatg tatgatcgaa ctcaacggcg
    28561 aaccctttgg caatatgcga caaacctacg gcggtgattc tctcaacacc gctacctatt
    28621 tggctcgggt gagttcgcct gaacagattg aggtgcgtta tatttctgct cttggtacgg
    28681 ataaactcag tcaaggtatg ttggcagcgt gacaagcacc gccagccggg gctgtatctg
    28741 attcagttgg ataagcaggg cgagcggaca ttcctgtatt ggcgaaatca atctgccgcc
    28801 cgttatttgt tgcaacaccc cgactattca aaagcactct cggcgttaca cgaggtggat
    28861 atggtttact tgagcggtat cagccttgcc atcttgccgg aaaacgaccg cactttgctg
    28921 attgcacagt tgcgtgagct caaaacacaa ggggtgaaaa tcgcctttga cagcaattac
    28981 cggccgaaac tgtgggaaag tgtggctgct gcccaagctc actatgccga gctgttgcaa
    29041 ttagtcgatt tagctctcgt gacctttgat gacgagcaac tgctttgggg cgatatgaat
    29101 gagcaagcca cctttgaccg tctggctcaa tatggcattc ataccattgt ggtgaaacaa
    29161 ggctctctgg gggcaacggt gttgcaaaac ggggagcgta cttttgtgcc aactgttgcg
    29221 gtagagaagg tggtcgatac cacttccgcc ggcgattcct ttaatgccgg cttccttaac
    29281 ggttacttat tgggtaaaga tgtcgttacc tgttgccaac aaggaaatgc cgttgccggt
    29341 atcgtcattc agcacaaagg ggcgattatt gccaaatccg ccactactca tttgaaatcg
    29401 cagtttgaac aatccattta gaccaacaat agcacaacga caagcggtta aatttgcaaa
    29461 attttttaca aaagcgaccg cttgcaatac aacataatga ggctagaaat gcgttacacc
    29521 accgaacaaa tcattgaaaa attaagacaa ctgaaagttg ttcccgtgat tgctttggat
    29581 aaagcagaag atattttacc gctggcgaaa accctttccg aaaacggttt gccggtggtg
    29641 gaaatcactt tccgctcaga agcagctgaa gaggcaatta aattagtgaa agccaactat
    29701 ccggaagtat tgattgcagc aggcacagtg ctgaccaaag agcaagtctt aactgccaaa
    29761 aacgctggag cggattgcat tgtgactccg ggttttaatc cgactatcgt taagtattgc
    29821 caagtgttag gtttgcctgt tacaccgggt gtgaacaacc cgatggcgat tgaagccgcc
    29881 ctcgaacttg gcatacaagc agttaaattt ttccctgccg aagccagtgg cggcgtgaaa
    29941 atgattaaag ccctgctcgg cccttacggc aacttacaga ttatgccgac cggcggcatc
    30001 tcgccacaaa acatcaacga ctacctcgcc attcccaacg tagtcgcctg cggcggctct
    30061 tggtttgtgg agaaatccct gattaaggaa cagaagtggg aggagattgg gaaattgacg
    30121 aaggaagtgg ttgagttggt gaaataacca tttaaagttc caagcaatga gctattttct
    30181 acccaaaata gatctctttc ctcttattta caaagcataa caaatattgc atactttatt
    30241 tagtagcata tagtaggtat atttaaagct atatatttaa cttaatgaaa aatatagcaa
    30301 aacaacctca aaactcttaa ctaaaggcta ttgaaaaata gccttttcta ttcaaataaa
    30361 agatagcttt atgaaaaaaa tagtctcaac gcttttaatc cttacctcaa acctggcatt
    30421 tgcggaaacc ataccggctc aacttcaaga aaaaatggat aaccagcgaa tgcaggaaaa
    30481 ccctacatta gaaaagcctg aacctcaagc caaaccacaa gcggttaatg cacaggctca
    30541 ccggcaatcg atctctattt caagccaaca gctgcttcaa cagcctgagt tgctcaactc
    30601 ggcaatgcta actgcgttgg tcactaacca cactgagaat gttcaattct tactgcctat
    30661 ttatcaacaa ttacccgcca gtgaacaaaa cagcgaagtt gtacaatggg cggaggcagt
    30721 taatgccaaa gcaaaacaaa attattcaga ggcaataaaa ggctaccgtc agctactggc
    30781 aaaataccct gaaaatcagc caatccgtat gcaattagcg gttgcattgt ttgaaaatcg
    30841 ggaaaatgaa gccgcagagg ctcaatttta taagttacaa gcgggcaaat taccgcaaga
    30901 aattgcaaat gcggtcaata gctatttgga ggcgattggc aagcaagatc aatggagttt
    30961 tcaaggcggc ttaacctatt tgaatgaccc gaatatcaac aatgctccaa atgcaggcac
    31021 gacctatggc aactggactg caccgaaaaa agaatccgcc caaggggtcg gctttcactt
    31081 tgaggcggat aaaaagtggt cttggggcaa tggtttcttc cacgagtttc gtttaaacgg
    31141 aaacggcaaa tattactgga ataacaaaaa atataacgaa tattcgctca gagaaagcct
    31201 cggcttgggt tatcaaaatg caaaacaaaa aatcaccttg ttgccgtttt ttgagcagat
    31261 gtggtatgcc agtggctcaa gccaaaccga aagcaccaaa cgctactcca acgcaggcgg
    31321 tgtgaatgcc cgctggcaat attggattac tccacagtgg caatctgcaa tcagctacga
    31381 atatgcggaa cagcgttaca ctcgccgcca acatttagac ggcaattatc attttattgc
    31441 cccgagcctc tatttttacc ctaacagccg gcaatattgg tttgtggggg cgaattttaa
    31501 ccgcaccagc ccccgtgatt tagatgatag ctttattcgc cgaggtgttt ctattggttg
    31561 gggacaagag tggaaaaacg ggctttcaac ccaattaagt gccagctacg cccgcaaaca
    31621 ataccgagcc ccaatgccga ttttccaaat tacacaacgt aacagtgaat atggtgtgca
    31681 agccagcatc tggcacagag ccttgcattt taagggtatt acccctcgct taacgtggca
    31741 atataacaag gtaaaaagca atcatagctt ctatagttat gacaaaaacc gtgtctatat
    31801 tgagttttca catacgttct aaacaaaaac gccccttaaa cggggcgatt tcttagttat
    31861 tttttcaatt taccccaaca attttttcaa gcgatacaac tcgtccaaag cctgtcttgg
    31921 ggtaagttca tcaggctgaa gggctttcag ggctttttca acttcatttt ctactacaag
    31981 cggttgaatt gcttcatttt tttgcaaatc aaaaccatcg gaaaacaaca aatccgcctg
    32041 tggatgttcg tttgcttgtt tggtttggtg ggaaatttcc tccaaatgtg ccaaacgctg
    32101 ctttgccagt tgaatcactt gctttggcac acccgccaag gctgccaccg ccaagccgta
    32161 acttttgctg gcggccccct cttgcaccga gtgcataaac acaatactgt cttgatgttc
    32221 acgcgcgtct aaatgcacat ttgccacccc tttgagctga ttcggcaagc cggtaagttc
    32281 aaaatagtgg gttgcaaaca aagtgagtga ctgggttttc ttcgccagcc attcggcaca
    32341 cgcccacgct agcgataaac cgtcataggt tgaagtgcct cgcccgattt catcaatcaa
    32401 caccaagctg ttttccgtag cttggtgcag aatatttgcc atttcggtca tttccaccat
    32461 aaaggttgag cgaccagagg ctaaatcatc gcttgcccca atacgggtga aaatgcggtc
    32521 aatcgggccg atttccgcac tttctgccgg cacaaaactg ccgatataag ccatcagcgt
    32581 aatcagggca atttgtcgca tataggtgct tttaccgccc atattcgggc cggttacaat
    32641 caataaatgc cgttgggcat ttaaaaacac cggattggca ataaacggtt cggttaaaac
    32701 ctgctccacc accgggtgtc tgccgccttt gatattcacc attcgcatcg ggctaaagct
    32761 cgggcaaaca tagcctaaac tatcagctcg ctcggcaagg ttggttaaga catcaagttc
    32821 agacagcatc atcgccgcta attgcaattc gcctaagcga ggcattaaca catcaaacag
    32881 ttcatcataa agctgttttt caagggcaag cgatgcccct tttgctttca gcactttgtc
    32941 ttcgtaagtt ttcagctcag ggataatata ccgctccgca tttttgagcg tttggcggcg
    33001 aacatagtga atcggggctt tgtgtgcttg cccttggctg atctgaatgt aatagccgtg
    33061 aaccgcatta aagccgattt tcagcgaatc aatgccggtg ctttcacgtt ctctgatctc
    33121 caaattttcc agatattgcg ttgccccgtc cgaaagttgc cgccattcgt caagctcggc
    33181 attataacct tctgcaatca ccccaccatc acgaatgagt tgtggtgggt tttcgataat
    33241 cgcccgctcc agcaaatcgt gtaacgggct gaaatccgcc agttgagcca aaagctgatc
    33301 gatactagcg gtcagatttt gtgaattttt tgcaatttcc ggtaactgag ccaatgcagt
    33361 gcgtagtcgg gtgaggtcac gagggcgagc cgtacgcaac gccactcggg cgagaatccg
    33421 ctccatatcg ccgacttgtt gtaataacgg ctgtaaatcc gacattcgct catattgtat
    33481 caatgctgtt atcgcctttt gtcgggcttg taatttacca caatctcgaa tcggctgatg
    33541 aatccaacgc ttgagcagac ggctccccat tggggtcaca catttatcta acaccgccgc
    33601 cagcgtgttt tccgtgccac ccgctaagtt ttgggtcagc tctaaattgc gacgagtagc
    33661 tgcatcgagc aaaatagtat cgctatgttg tgccaaatga attgcgttaa tgtgcggtaa
    33721 agcggtgcgt tgcgtttctt gggcataatg caagacacag ccggcagcac aaagtgccac
    33781 caccgctttt tccaccccaa atccggcaag tgtttgcgtg ccgaattggc gatttagcag
    33841 ctgaatcgcc gttaccagct caaattccca caccggacgg cggcgtaacc ctttgtaatt
    33901 cgttaaaatt gaggcagcgg aaaaatcttc cgcatacaga atttctgccg gttgtacacg
    33961 ttgtaactcg gcggatagag cctctttgct agcaagttca ctgattaaaa accgcccgga
    34021 ggtcatatct agcgtagcaa tcgcaaatgt gcctttttct tcgtaaactg ccgccaccaa
    34081 gttatcttgg cgttcgggta acaatgcctc atcgctaact gttcccggcg taacaatccg
    34141 taccactttg cgttctaccg gcccttttga ggttgctggg tcgccaatct gttcacaaat
    34201 tgctaccgat tcccccaacg ccaccaattt cgccaaatac ccctccactg cgtgataagg
    34261 cacacccgcc atcggaatcg gctcaccggc agattgccct cgcttggtga gcgaaatatc
    34321 caacaatgct gccgctcgtt tcgcatcatc ataaaacagc tcataaaaat cccccattcg
    34381 gtaaaacaat agtatgtccg gattttgagc tttgagttgc aaatattgtt gcatcattgg
    34441 cgtatgggtg gagaaatctt gtgtcatcag tctatttatt gtgagaaatc attgctgata
    34501 gtttaacaga ttcagccaag ggataaaaac aagcggtcat ttttcgcaga aaatttgcaa
    34561 tggtcagccg ctgttttttt gttacaatgc accaaatttt taaaacagag gtcagaatgt
    34621 taagaaaaat agtgctactt tctacaggct tattattggc ggcttgtagc agcaatgaac
    34681 cgcaacaacc ggaagcattc ccgggcgaat ttgccaatgc cgattatgtg ctttcggaag
    34741 aaaatgctca aaaatgggta attgccagcg aacaggcaaa acagtgtatc taccccaatt
    34801 taacccgtat tcagcaagag catttttcca aagaagattc ttatattttc tcacaatatg
    34861 tatttttcta tccgcttgaa gagattatcg gggagcagta tgtaaaaatc attcaaaatg
    34921 atgaaaaatc aatgggttac gctcaatatt tattcaaaaa actgaaaaat aacgaagcgg
    34981 ttgaagcgtt accgaaaaaa caatgtgcga ccttacgcac caatgcaaaa aatgatttgg
    35041 cagtggtaaa agggcagtat caaagcggta tgatcgaaga aaccaatagc caagaaggga
    35101 aatcaggcga taaagttgcc accaatgata ataaattctt cttcgatatt atcaaatggg
    35161 gcagtatgtt acttctctag tgagcctcaa tactcggttg ttttaaccac aaccgagcca
    35221 tttataccta ccgattgaac ttttatctaa agcctcagtc ttaaaataca gtttttttca
    35281 attattttcc ctcttttata tttaaggatt ttcaataaaa gcattaaaaa cattattagc
    35341 attgggcgtg gcaatggctg tttcagctac ttcatttgca caaaccactc aacctactgc
    35401 ggctgaacaa atgaacgcag ttaaagcctc tgttgccgaa aaagcaactg tggctaaaga
    35461 agcaacaaaa gagaaagcag cggagttaaa agccggttct gcggaaaaaa tggcgacagc
    35521 aaaagccaaa accgaatcat ttaaagattc cgctaaaaac aaaatggacg cagtaaaagc
    35581 caccacagca gaaaaaacgg acgtgattaa agcgaaagcc gcatctgcaa gcgacacaat
    35641 gaaagtgaaa gctcaggaag caaaagccgc caccgcagag aaagctgcaa ctgctcatga
    35701 aaccttaaat tcagccaaaa ccgcaaccgc tgaaaaagcg gccccaatga aagcagctgc
    35761 aacagaaaaa atgtcagcag tgaaaaaatc acaaaaagtg aatatcaaca ctgccaacga
    35821 agagcaatta caatcgctta ccggcattgg tgaagcaaaa gccaaagcga ttgtagaata
    35881 ccgcaaaaaa cacggtaaaa tcaaaaatgc agcagaactg gcgaacattc ccggcattgg
    35941 cgatgcaacc attgagaaag ttgctccata tttaactttc taatgttctg caagcggtta
    36001 ttttgttgca aaattttgca gaaaaataac cgcttgttca tttcacacct tgtattctta
    36061 aatttcaccc ccattaagtg aatcgtattt ctttcaatac agtattcaat aaactcaaaa
    36121 ggaacagaaa atggcattag atttcaattc agttggatta gattcagtag aagcaaaagg
    36181 cggttacggc atcggtttac aaattggtca gcaactttta ggcagcggaa tggacgtaaa
    36241 cgcagaagcc gttgcccgtg gtatttatga tgtgttaaac aacaatgagc cggcaatcga
    36301 catcaatgaa gtgaccgctg cattacaaga attaggtcag cgtgcggaag cggctcaagc
    36361 agaagcattc aaagcgattg atgccgaaaa caaagcgttc ttggaagaga ataaaaaagc
    36421 aaacggtgta atcgttaccg acagcggttt acaatatgaa attttaaccg aaggcacagg
    36481 cgaaaaaccg actgcgactt caaccgtgcg tgttcactac accggttcat taattgacgg
    36541 caccgtgttt gacagctcgg taaaacgcgg tcagccggca gaattcccgg taaacggagt
    36601 aattcgtggt tggacagaag cattgcaatt aatgccggtt ggctcaaaat ggcgtttaac
    36661 cattccacaa gaattagcct acggcgaacg tggtgcagga gcatcaattc caccatttgc
    36721 gaccttagtg tttgaagtgg aattgcttga tattctctaa tcaaactcaa ataaaaaacc
    36781 tgtgaaggca tttcacaggt ttttgctatt taagataact atattatgca gaaaaaatca
    36841 ctcatatctc gcttacttaa aaatattttc ctctacggca gcctgtttat cattattagc
    36901 ctggtggttg attggtatcg ctcgccctcc gctccggcac aatttgagca aaccgtgcat
    36961 tatgatattc aacaccagcc gaaagtaatc gcccaattaa gccacgagaa gccgatgcta
    37021 ctctattttt ggggaagttg gtgccactat tgccaagtgg tttcgccgaa tatccaacaa
    37081 ttagcgaatg acggcacgga agttttaggt gttgccctga aatccggcga tgaaaatgac
    37141 gtacaagcct atctcaatga aaatggctac actttcgcta ctttgaatga cccaagcggt
    37201 gatttctctc gaggttggaa cattcaagcc accccaacca tcttgattat caaagacggt
    37261 aaaatcgccc accacaccac cggctacact agctatttga gcttaaaatt cagactatgg
    37321 ctggcagacg tgttgtatta aacgattcct ttcatttgca aaaaaatcca ctaaatccga
    37381 ccgcttgttt acaccaaaaa gacaagcggt actaaattca ttgtcatcat tgtggtgatc
    37441 gataaaatca gcctcgatga gccaaaaata aagcctcggt ttttgccgtt gtcgaatttc
    37501 accagtaaat ttgccattgc tgtgagggcg tacacttcca ccaccgtaaa aatcacggtg
    37561 taaatatagg cagcggtcag agttgcgact tttaccgcaa taaaccaagg ggcaataatc
    37621 gccaccgcta ataacggcga aatccaaatt aattgctgtt tggtgaggtt gattttacga
    37681 gaaaaagtcg attgcaaact gacggttaaa acaccgttga tgaataacgc aatcggcgaa
    37741 tgctccggtg cgtgcaggac attgtcgaat aaatacggaa aaataataaa ataggacgat
    37801 gccacactca gtggcacaaa tagcattaag tgagcgaaca aaaactcttt gttgatgatt
    37861 tccttaattt gtgcgaaacg aaatttcacg ccgccattca cgccttccac cgaataaaac
    37921 ggcttgacca taaacgcaaa caagccggct tccaaaataa agcagatcca aatcagcgtt
    37981 tcaatcatct caaacctaat aaacggaata ataatcagcg gggcgagcat tgaagacata
    38041 ctggtgactt ttagatattt gccttgtaaa cgggttttag cagcatattc atcgccggca
    38101 atcgccagta aacaggctct ggcattagtg ccgaataagc aactccccaa cccaaagcaa
    38161 gtggttgcca acaataaaat gaggtaatta tccgctgcta aaaacagcat ataggcaacc
    38221 acatctaata aacagcccaa taacatcatt tttgccaagc cgtagcgatc cccccaaatg
    38281 ccggctaaaa ttgccaatgc ctgattacaa aacaacagca atgaaaggaa aaaagcgatt
    38341 tgggcgttat ctagcttaaa taaaagaaaa acacgctttg cattgaaaaa aaggcggtgg
    38401 tggaaataaa acggcgtagg agtaatagtt tttctaaaga cattcagaat ggttgaattg
    38461 gttaaataaa gagcggttat tctacctttt aagtcaaccc caatcaatcg gcttttgttt
    38521 tacctttggc gtgggtttcg gtatgataag cgatatgtag tggcgggtcc tgtgcccgcc
    38581 taaataccaa tgataacttg cgggcgagca caggactcgc ccctacgata aacaagaaaa
    38641 tgaaagacac cattgttgct caagcaacac cgattggcag aggcggtgtg gggattctgc
    38701 gagtttccgg tccgcttgcc gaaaccgttg ctcaagccgt attaggcaaa acattgccac
    38761 cccgtatcgc aaattatttg ccgtttaaag acgaagatgg cactgtgttg gatcaaggca
    38821 tcgccttatt ttttaaagct cccaactcct ttaccggcga agatgtgctt gaacttcaag
    38881 gacacggtgg gcaggttatt ttagatttac tgctcaaacg cattttacaa gttaaggggc
    38941 tacgcattgc ccgagcaggt gaattttccg aacaggcatt tttaaacgat aagctcgacc
    39001 ttgcccaagc tgaagcgatt gcggatttaa tcgacgccac cagcgagcaa gccgctcgtt
    39061 cagccctgaa atcactacag ggcgaatttt ctaataaaat caatgcgtta gttgattcag
    39121 taatttattt acgcacttat gtggaagcgg cgattgactt ccccgatgag gaaattgact
    39181 ttctggcaga cggtaaaatc gaggcaaaat taaatgagat tatcgctcaa ttagacggtg
    39241 ttcgccgtga agcgaaacaa ggctcgattc tgcgtgaagg aatgaaagta gtgatcgccg
    39301 gtcgcccgaa tgcagggaaa tcaagcttgc ttaacgcctt agccggacga gaggcggcga
    39361 ttgtaaccaa tattgctggc acaacccgtg atgtgttgcg tgaacatatt cacattgacg
    39421 gaatgccgtt gcatattatc gacaccgccg gtctccgtga tgccagtgat gaggtggaac
    39481 gtatcggcat tcagcgtgct tgggaagaaa tcgcccaagc cgaccacgtt ttattaatga
    39541 ttgacagcac cgagcaacaa gccgatcagt tccgacaaga gtgggcggaa tttctagcca
    39601 aactaccggc aaatatgcct gtgaccgtaa ttcgcaacaa agtcgattta tccggcgagc
    39661 aggaaggctt aattcaggtg gacgatttca cgatgattcg cctctctgcc caaaccaaag
    39721 tcggcgttga tttattgcgt gaacacttga agaaatcaat gggctaccaa agttctacgg
    39781 aaggcggatt cttggctcgc cgccgtcatc tgcaagcatt ggaaaccgct gccgagcatt
    39841 tagagcgagg gcatatccag cttactcaat tcttagccgg cgaattgtta gcggaagagt
    39901 tgcgtatggt gcaaaatgcc ttaagtgaaa ttaccggcca gttcacctct gatgacttat
    39961 taggcaacat tttcagctca ttttgcatcg gtaaataaat gacacttttt aaccgctttc
    40021 aaacccagtg tgcaacgcat ttgccgaacc aaaccgcctt tttaatcggc ttaagcggtg
    40081 gggtggattc ggtggtacta ctccaccttt ttgcccgcac ctcatttcac gttagggcaa
    40141 tttatgtcca ccacgggcta agcccaaatg cggacgactg ggcagccttt tgcgaacaat
    40201 attgcaagcg gttaaatatt ccctttattt tacaaaaggt cagagtggat agctcaaacg
    40261 gcgttgaaaa cggggcgaga gaagctcgtt accaagccat tcaacaacag cttaagccca
    40321 atgaagtgtt cgccaccgcc caccatttag atgaccaagc cgaaactttt ttgctcgccc
    40381 tcaagcgagg cagtggagtc aaaggattat cggcgatgca agcggtcact tttttgcaaa
    40441 attttaccgt ttttcgaccg ctactcactt ttactaagtc agatttgatg ggttatgccg
    40501 ttcaacatca gctaggttgg attgaggacg aaagcaatgc cgataaccgt tacgaccgta
    40561 atttccttcg caatagcatc ttgccactac tcaatcaacg ttggcagcac ttcagccaaa
    40621 tggttgcccg ttccgctcag cattgtgcag aacagcaagc cttgatagaa gagctattaa
    40681 gcgatgaact caagtcaaga acaggggaaa aacaacaact caacattaac ggctttgggc
    40741 aattttcact tgccaagcaa cagcaactta gccgcctttg gctggaacaa aacggagtga
    40801 gaatgccaag ccaagcccag ctacaagcgg tcatttctga actgattttt gcaaaagccg
    40861 ataaaaaccc acaggttaaa gtcggcgaaa atattatcag acgctatcaa caagctattt
    40921 acgttaccga agagatcgcc gacattccgc catttgaggt agtacttccc acagaaacca
    40981 aagtggaatt actctatcag ctcggtacta ttattcgcca acaacaagag atcatttgca
    41041 aaaaaaacgg gaaaaatcac cgcttgcaac tgccccaaga gctggctcaa tcaactattt
    41101 ctattaaaat cgggcaacta ggcaaagtaa aatgctacgg caaggctcaa cgggaagaaa
    41161 tgaaaaagat ttggcaaaat cacggcatac ctgtttggga acgcaaccgt acaccgctga
    41221 ttttttggca agacgaatta gttggcctat tacctatcct ctagggtgaa attgctgatg
    41281 aagcgatttc aaacgggctt ttgccacttg agtgtaaatc tgggtggtcg ataaatcact
    41341 atgccccagc aacatctgta ccactcgcaa atcagcccca tgattgacca aatgggtcgc
    41401 aaaggcgtgg cgaagcacgt gaggcgagag tttatctgca tcaatacctg caagcacggc
    41461 atagtgctta atccgatgcc aaaacgtctg ccttgtcatc tgctgccctc gccggctcgg
    41521 aaatacaaca tcagactgct gattattcag caaaataggg cgaccgtact ggaaaaactc
    41581 ctgtatccaa taattcgcct cctcgcccaa tggcactaac cgctctttat cccctttacc
    41641 gataattctc accccccctt gtttgaggct gatattatcc atcgaaagcg acaccagctc
    41701 cgttacccgc agaccggtgg catacagcag ctccagcatt gctttatccc gtaactctat
    41761 cggatctaac gtattcgggg catctagcaa atccattacc tgatcttcac ttaaggattt
    41821 gggtaaagaa gcaccccgtt taggggaaga aagggttaaa gtcgggtcat cttggcgata
    41881 atgctcaagg cataaaaaac ggaaaaattt ccttaaacaa ctcaacattc tggcagagct
    41941 tgtcgcctta tagccttgct ccaaacgctc gccgagaaaa ccttgtaagt cgatatgatc
    42001 gagagacaga aatgctttag gttcagaaaa ataatcggaa aaacgctcta agtcagaacg
    42061 ataagaggca acggtatttt cagacaaacc gtgttcttgc catagtgtat cgagaaattg
    42121 ttcaataata gggtcaagct cagccataca agcgggtata ttttcaggaa attttgcaaa
    42181 ttttaacaga attaaatcta taaaaataca aaagcgatag aaaagaaaaa accgattagc
    42241 ttatgctaat cggttta
//
LOCUS       DANKBV010000005        41344 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.005, whole genome shotgun sequence.
ACCESSION   DANKBV010000005 DANKBV010000000
VERSION     DANKBV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 41344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41344
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.005"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..554)
                     /locus_tag="PWY43_000193"
     CDS             complement(<1..554)
                     /locus_tag="PWY43_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HDL2135112.1"
                     /translation="MQFFSMNILERYIGKTILSAIFLTLFMLIGLGAIIKFVEEFRDV
                     GRGSYDGLKAAYYTFLTIPRDIETFFPIAALLGSLLGLGNLASRSELVVMQASGFSRF
                     RIGLAVMKTALPLVLFTMIIGEWGVPQTEQFARNMRSIAQSGGSMLATKDGFWAKDGN
                     SFIYINHIKNEKSLDNVLIYQFGEG"
     gene            complement(554..1663)
                     /gene="lptF"
                     /locus_tag="PWY43_000194"
     CDS             complement(554..1663)
                     /gene="lptF"
                     /locus_tag="PWY43_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDL2135113.1"
                     /translation="MILSRYLTKEIFKSQLAILFILLLIFFCQQLVRVLSSAVSGKVP
                     ADLVVSLLGLGMPTMAQLMLPLSLFIALLLTLGRFYAESEITVMRACGIGQGLLVKVA
                     MFLSIFTTALAVYNVFWLTPWAINKQSEMLAEAKANPRFAALSAGQFMSAGGYVLFID
                     KIENNSLNDIYVFQPEQQKKNKPSVVVAESGTLQGLPNGDQVLTLAESKRFEGTPQSA
                     DFRISHFENYSAYLGYQDVESDEKLVQRADFKKLMETKTPEAKAELHWRLALILAVPL
                     MALLAVPMSSVNPRQGRFAKLLPALLLYLIYFLLQSSLKSSGASGKLNAEVLMPLVSV
                     AFLVLGVILNAWDSKFMSKLRYQFGAKKLTNKAVA"
     gene            complement(1837..2634)
                     /locus_tag="PWY43_000195"
     CDS             complement(1837..2634)
                     /locus_tag="PWY43_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135114.1"
                     /translation="MRFWGKTFVVGLIGLGTQNTIFANDDAQISRYEQAKLRLSYDGN
                     VGNLLQQLSQRLKVGFIAYDVDITRKISLQNETETAIKTINEQIESQLTNIDVRFEKI
                     GERLFLVVSAKDVAPLLQNEPKEVQFVGDVIFDGEPNLTEQAQSTAEATKVQNILNIA
                     TNQEKIMAAKGKKAPQYKTVTKDNIGLKNLRVTALGTFLVFDTSVDAAKLRVKGNFED
                     IAQGENIIAILHQNTEAPPKIEIEDSQGKKLILEAHKATSSKKSKKQ"
     gene            2872..4368
                     /gene="pepA"
                     /locus_tag="PWY43_000196"
     CDS             2872..4368
                     /gene="pepA"
                     /locus_tag="PWY43_000196"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDL2135115.1"
                     /translation="MEFSVKNGSVEKQRTACLVVGVYEPRRLCSAAEQLDKLSNGYIS
                     TLLRRGDLEGKAGQTLLLHNVPNVLADRVLLVGCGKERELTERQYKQIIQKSVQTINE
                     TGSMEAVSFLTELHVKGRTPYWNVRFAVEAIQEQLYAYNDFKSIKPEVRRELRRVIFN
                     VAHRKDLPDAERALEHGKAVALGISHAKNVANCPPNVCNPHYLAELAKGLAYDYPAIS
                     TTIIDEKQMAELGMNAYLAVSRASENEAFLSVIEYKGNPNADAKPIILVGKGMTFDSG
                     GLSIKPAEAMDEMKYDMCGAATVYGTIKAIAEMKLPLNVIGLLAGCENAVDGKAYRPG
                     DILTTMSGLTVEVLNTDAEGRLVLCDALSYVERFEPELVIDIATLTGACMIALGNHNS
                     GLMSPHNGLANELLAAAEQADDKAWRLPLGEEYQEQLKSNFADLANIGGRLGGACTAG
                     QFLSNFTKKYTWAHLDIAGTAWKSGAAKGATGRPVSLLTQFLINKAQV"
     gene            4542..5489
                     /gene="cysK"
                     /locus_tag="PWY43_000197"
     CDS             4542..5489
                     /gene="cysK"
                     /locus_tag="PWY43_000197"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL2135116.1"
                     /translation="MTIYADNSYSIGNTPLVRLKNFGNGNIVVKVEGRNPSFSVKCRI
                     GANMVWQAEKDGILTKDKEIVDATSGNTGIALAYVAAARGYKLTLTMPETMSTERKRL
                     LRGLGVNLVLTEGAKGMKGAIAKAEEIASSDPERYVTLKQFENPANPAIHQQTTGPEI
                     WDATEGKIDVLVAGVGTGGTITGVSRAIKQDKGKQIISVAVEPSESPVITQTLNGEEV
                     KPGPHKIQGIGAGFIPKNLDLSLLDRVEQVSSEEAITTARRLMAEEGILGGISSGAAV
                     AAADRIAKLPEFADKLIVVILPSASERYLSTLLFEGIEA"
     gene            5675..6937
                     /gene="rho"
                     /locus_tag="PWY43_000198"
     CDS             5675..6937
                     /gene="rho"
                     /locus_tag="PWY43_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL2135117.1"
                     /translation="MHLTQLKNTPVSELVEMGENQMGLENLARLRKQDIVFAILKQHA
                     KSGEDIFGQGVLEILPDGFGFLRSSDSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKI
                     RPPKEGERYFALLKVDSVNDDKPEASRSKILFENLTPLHANSRLKMERGNGSKEDLTA
                     RILDLAAPIGKGQRALIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVT
                     EMQRTVRGEVIASTFDEPATRHVQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYN
                     TITPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVDTGSKMDEVIFE
                     EFKGTGNMELHLSRRIAERRIFPAIDFNRSGTRKDDLLMAADELQKTWMLRKVLNPMD
                     EVDAIEWLIDKLMMAKTNEEFFEIMKRS"
     gene            complement(6998..8116)
                     /locus_tag="PWY43_000199"
     CDS             complement(6998..8116)
                     /locus_tag="PWY43_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2135118.1"
                     /translation="MKKIKTTLSALALSLSLISVNSWAIPADYLSQDNISQYDKAFNF
                     VLAKVLPAPKGKEDGRGAPIEHLVIQYAPDYVDDPYLKEDVLNFRRWYWQAMYASLDQ
                     KVKEKPTYLNTYRLQAEAYFVNKEYREALSQLDQILRQNPRDVHALAMTVLASRVLED
                     TEQETARLSALKQLAPEVAQRMTDFFAFTEQTLAASYGNEPQTAMIPDTIAVFGSTPN
                     KDGTPSPLLLDRLQKTKEMAQKFPQAKIIVSGGAVKTEFAEADVMAKWLSENGIEKDR
                     LLLDPVARDTPGNAVGIIKLMQTYNGKKVLGIGTLQHIPRATAVLKGYAQHIGYPIEM
                     DSVGGGKPATPGKAKIEALYTYVNVARSMGLFELGDFK"
     gene            complement(8129..10276)
                     /locus_tag="PWY43_000200"
     CDS             complement(8129..10276)
                     /locus_tag="PWY43_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL2135119.1"
                     /translation="MKRLRYSYLALILGSALPLSAYADGYLSNDNIRQFDARFNFFLA
                     NPLPAPIAKADGRGEAIENLVVKYASDYLNDIYLKEDVIRFRRWQWQDLYTSLDLKTK
                     QKPDYLNTYRLQAEAYFINKQYQEALSQLDQILRRNPNDVHAMAMSVVASRVLENPNT
                     EAERLLALYQISPSIAQKVQQYLHFTDTMLAASYGSEPQTTMIPDTIAVFGQSPNPDG
                     TPSKGLLQRLEKTKEMAAKFPNAKIILSGGPVRYIYAEADVMKKWLIENGVDESRLLL
                     DDVARDTPGNAVGIIDFMKEYGGRKVLGIGTILHLPRAMSVLKSYADSIGYELEIDSA
                     GGGSPPNEKNKKGEALYTFVNVARAMGLFTLGDFENLLAKEQAEKEQREALEKAQQNY
                     LEKLVAERDQAVKNAENSANELEVARQALETAKQAHQQAEEELNKELSKTQEQLKQST
                     EALSRLIIKQNNPFSEHYVVQLNLMEQATYDLYRRLAEIEHTQDWNLWLSDSLSKYRL
                     KNRSTEDYHLLHIGLDHQVSHNWSIGGVFGISQGQIENRTHKVKGTSAGIYAGYRPTS
                     GLFWTNFFNYSHFTNQSGVTKRDNNAYSLSSEIGRNIVMSHQWQITPKLQLGWAKAGS
                     ARMFWAGTGLRLGKVIELNAIKLKPYADISYRFRNRHIDTLDSLSDRHRNVISIGVQA
                     VGNRHYFAIEGSMLHGDALKRSYGLHANYHYQW"
     gene            complement(10276..12219)
                     /locus_tag="PWY43_000201"
     CDS             complement(10276..12219)
                     /locus_tag="PWY43_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2135120.1"
                     /translation="MSQDIQADLKKILALLSRNRRFQSLFLLLLRSEQFINSDALSTE
                     LQLSVRTVKKEIKLLKEKLEPYGIQIFSRPFYGYKLLIQFEELHTQLKQYFQISQPMT
                     VNNKFESRVNAILRRLLVSQRALKVEDFQSELYLSFTSALTKEFSQVKALLGKYGLIL
                     EAKPYHGMNIVGESYRKIMLTVRLYKYFTQLEHPFGIKEFEQCFACPMTEKIRERLAK
                     TIIHFNLVFSDIYAERFIIYLRYFYNQQPELLLPKLNFNYQQTNEYQFVIKLLEQLRE
                     QDSRFEFNPEVIRFLTYIAIASTDLYRFKDCNSDNYPLLLDKAWEIHRFIFTQFSAYL
                     NLSEIHDDTCRKDLLKMLIPIAMKMMLSISDDVDLGLFNQTELHRNPILAHTVEKLVD
                     DIEKNYGYSLSIREQYFIFDIFSGMVNRIELERKPLRLAIIALNGRLSTQQLKRNIKR
                     YFSDYILKIETKVLYELAIMEDKPYDYYLCNEYGKNMAIPYKPIYFADSSLNENEYAT
                     SLNEVFYHAYQYDEVLPSIKSIEIEAQYRLNEFHLEQEYLNYEQIILENKQDKIKLFY
                     QLNASVEKFEIYYFKNKEDFTLYGYTKFIVMSLNVIEQPQKLKMILNIINKIAQTNFI
                     LNQLCWLDVKSYKYFFTQYKEKS"
     gene            complement(12737..13804)
                     /locus_tag="PWY43_000202"
     CDS             complement(12737..13804)
                     /locus_tag="PWY43_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HDL2135121.1"
                     /translation="MEKFHMRYFAKHWYKTTACLTTIFTAFPLFAQPIENIPTSHYEL
                     EKIIIFSRHGLRSPLTEKGSDLEKSTPYKWANWSVPTGHLTHKGTLLETYFGQYLGQW
                     LTHNGLLDKASCESGKQIEVYANSLQRTIGTAQALVAGGFSGCNVQVKHHQQIGKMDP
                     VFNPIIRSDDQTFVQTALSKIDLNKTHQYLAPSYHLLSEIIDYAHSEKCLQEKQCEFF
                     NDVGKLRIKKDKEPGVSGTMRLSENIVDALLLQYYEGLPLKEIANNQLDSDQKWQTLN
                     KIKNDYQFMLRGDPFIAKHISLPLLKYIQQDLNSENKITLLVGHDSNIIALLSALNVK
                     PYKLAHQYEQTSIGGKVFIEI"
     gene            complement(13837..14874)
                     /locus_tag="PWY43_000203"
     CDS             complement(13837..14874)
                     /locus_tag="PWY43_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDL2135122.1"
                     /translation="MDSVYDIIRLEQGRYLFRQQPAQSVQIFFLDNPDSKDSKWLAET
                     DLAEFELKLSACEARPFFLIQSANGEQIVAERTLPVAGMNNFRDMGGYVAHQGKRVKW
                     GKLYRSDHLHNLRDEGVAYLDKLGIQTVIDYRSPNEVAKYPNPPINGREQTFRLDPNA
                     HTAELAAQFSADKHDEDRNLVNKIIAQKAEGNLINRYDIVMAQYRNFVEKAECQTAFA
                     EMLRLATDPENAPLVQHCRGGKDRTGFGAMLLLGILGVSKADIIADYMLTHDNRLARN
                     EEKMAIYRTFTQDQDVLDYLLSLIDTQPEFIEQSLNTIETQYGTIEQYAQRVLGITAK
                     EIEALRANYLA"
     gene            complement(14874..15680)
                     /locus_tag="PWY43_000204"
     CDS             complement(14874..15680)
                     /locus_tag="PWY43_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2135123.1"
                     /translation="MSNKITKQDLNKVFWRMQVLNVTNNFQSMQAIGFLSSFIPVLKR
                     LYKDRPQEERAEAMKRHLKFFNSHVNSDALILGIAAAVEETTKEDEKETVTAIKTGLM
                     GPLAGLGDSLLKFTWLPICGSIGAALALSGNVLGPILMFLLYNSVNIATKYYGIHLGY
                     SKGVDLIHGAGNNILQRLGNMANVVGLMVIGALIASVVKVKVVSQIAAGDNVIKFQEM
                     LDKVMPGLLSLLLTVVIYWILKKTNGKYAATMILATMGICIGLVYAGILG"
     gene            complement(15677..16444)
                     /locus_tag="PWY43_000205"
     CDS             complement(15677..16444)
                     /locus_tag="PWY43_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2135124.1"
                     /translation="MLTALLVACWAGICALDDIGTQMIRRPLLIAPVVGLIMGDLETA
                     LIIGATLEVMWMGIGNVGAYSAPDMISGTAIGTALGIAAGEASTAVALAVPTSLLAQQ
                     LLILYRSGVCALNPIAERWAASGDFDKIFRIHYIPMLIAFSIRAIPTFLAVYYGADII
                     AQAVSALPAKVITGLNVAGKIIPAIGIGLLMLMMIKKAELWTFLIAGFTLAVYLKLSV
                     LPITLIALPLALIYDLAVSKKAEAAPTKTADEEDYDL"
     gene            complement(16447..16914)
                     /locus_tag="PWY43_000206"
     CDS             complement(16447..16914)
                     /locus_tag="PWY43_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2135125.1"
                     /translation="MICFSRIDDRLIHGQVVTTWVNLHRIEQIIVLNDAVAADPTQKQ
                     ILTMAAPQGIKVVAFGIEKFGEVLQKTQITRRTMLLFTNSIDVLRTVELGVSIDKLNV
                     GGMRYQAGREQLSKAVSVTPEERQAFKTLLEKGVEVTVQMVPNDEKLNLQEII"
     gene            complement(16901..17332)
                     /locus_tag="PWY43_000207"
     CDS             complement(16901..17332)
                     /locus_tag="PWY43_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2135126.1"
                     /translation="MSNQIALLLMSHGSFARAALASAELIVGQQENVAAIGVVAVDNV
                     ADIKRQMEQTIAELNTDKGLIILTDIVGGTPMNLASSQLTHPNVFACSGLNLPLLLEV
                     LMSRDGNLQEIRENLPLAYQNGFSLRTQQDFVSQEDDDDLL"
     gene            complement(17515..18699)
                     /locus_tag="PWY43_000208"
     CDS             complement(17515..18699)
                     /locus_tag="PWY43_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDL2135127.1"
                     /translation="MTSSNTIKQLDKDYIAQTYGRFDLALSHGKGCEVWDFEGNKYLD
                     FTSGIGVNSLGWADEDWLQAVTTQAGKLAHTSNLFFTEPASQLAQKLVAASGLKRAFF
                     CNSGAEANEGAIKTARKYSFDKYGSGRSTVLTLVNSFHGRTISTLAATGQEVFHKHFF
                     PFTEGFEYLPVNDIEALKARLETENVCGILLEVVQGEGGVCPLEADYLHAVQSLCQEK
                     DILFMIDEVQTGIGRTGTLFAYQQFDLTPGLVTLAKGLGGGLPIGAFLLGEKVEMTLG
                     KSDHGSTFGGNPVSCAAANVVIDKINAEFLAQVQQKGQLLQQRLASLPKVKSVSGLGL
                     MLGVEFEEGILAADIVAKCINKGVLFLTAKSKLRLLPPLMISEQQIEQGIRVLEDVLK
                     DS"
     gene            complement(18712..19566)
                     /gene="argB"
                     /locus_tag="PWY43_000209"
     CDS             complement(18712..19566)
                     /gene="argB"
                     /locus_tag="PWY43_000209"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDL2135128.1"
                     /translation="MQQQEINQFANLLEKSTACLVKWQGKIIVVKYGGNAMINEELKK
                     SVMADLLLLHRLGVKVVLVHGGGPEISQGVKLLGKEPQFINGLRVTDSDTINVVLQML
                     AGKANKSLVALLKGKGIGLCGIDGAMLQCKKLEAEVDYGFVGDIVKVDPTVIEIALNA
                     GLIPVVSTVGVDENGQAYNINADTAASEIAIALQAEKLVSMTDIAGLLRDRFDESTLI
                     PEVEVSEVQSLIEQGIIAGGMIPKIACCTDFIRAGGSEANIIDGRIDHAILVEMFGGK
                     NGTRFYKK"
     gene            complement(19658..20599)
                     /gene="argC"
                     /locus_tag="PWY43_000210"
     CDS             complement(19658..20599)
                     /gene="argC"
                     /locus_tag="PWY43_000210"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL2135129.1"
                     /translation="MSKYKIFVDGAVGTTGLRIHERLSQADDIELLTLPEEQRKDLNA
                     RVAKVAEADLTFLCLPDEASKELVANAPENARICDTSTAHRVNPNWVYGFPELSGQRE
                     KIQNAKRVAVPGCHATGYIALVRPLVEKGILPADYPLSCHSLTGYSGGGKAMIANYEN
                     ENSPNAFNAPRVYGLTLQHKHLPEMQAVTQMQNPPIFTPVVADYYSGMLVTVPLPIFA
                     LQAVKSGEEIANLLADYYQTSRLIHVHPFQSLPEDGMLAANAYAGKGSLEIFIYGNST
                     QLLLAARFDNLGKGASGAAVQCMNIMLGRDEVEGLEL"
     gene            complement(20742..21359)
                     /locus_tag="PWY43_000211"
     CDS             complement(20742..21359)
                     /locus_tag="PWY43_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDL2135130.1"
                     /translation="MITLHYLNSSCSHRIVWLLEALGLDYELKIYHREESGFAPMEVK
                     ALHPLGKAPVLQDGDLVLAEGNAIIQHLIARYAEGKFEPTRGSEAYSMYHYWLSISAS
                     LFSANLVIMLANRSDLGAFGDYAKAQTPLYFNYIEQTLSDKKWIVGDELTGADFSLCF
                     PLQWGRNFVEMANYPNIARYLEQVENHPAYQKANQKALKLDMNRF"
     gene            complement(21443..22567)
                     /locus_tag="PWY43_000212"
     CDS             complement(21443..22567)
                     /locus_tag="PWY43_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL2135131.1"
                     /translation="MINLFTPYTLNNDVEIKNRLVVAPMTHSASNDDGTISEQERHFL
                     HNRAADMGLFITAATGVMRNGKAFYGQPEALDERYLASLTETANILKNQGTKAILQIF
                     HGGAQAIPALLDGANAVAPSEHANGAKALTADEVKQMIQAFANATDLAIQAGFDGVEI
                     HGANGYLIQQFFSTEFNQRTDEWGGSLEKRLRFPLAVVEAAVSVREKHQRPDFIIGYR
                     FSPEEPGEHGLTMTDTYALIDALCEKPLQYLHISLWDFGKKARRGADTNLTRMQLVHQ
                     HINGRLPLIGVGNLLTGEQIRKAYATGWAEFIALGKAVMLNPNVATLLKDNRDSEIVS
                     ELDPQQADHYGIPDRLWGYCVQGGAWLPPLKGQNWQPMDI"
     gene            complement(22631..23311)
                     /locus_tag="PWY43_000213"
     CDS             complement(22631..23311)
                     /locus_tag="PWY43_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDL2135132.1"
                     /translation="MKMLDFETTVKTRQSIRKFLPLPMTETEIKQILADAQNAPSACN
                     TQPWLVHVASGETLARLKARFKKTFEAGINDADFEFNQTKFTGDYEPRWRNQYSHVFT
                     TSYGIPREDKAGRQVVYDDNIVGYGAPHMAFLFMPSIDGHDVNIASDVGMYSQTFLLS
                     LTARGFGGIPQLALAMFAKDVREELGIPEHYKLLHAISFGHPDWDAMQNKHHLGRVPV
                     EESATLHW"
     gene            complement(23328..23759)
                     /locus_tag="PWY43_000214"
     CDS             complement(23328..23759)
                     /locus_tag="PWY43_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2135133.1"
                     /translation="MKIFYHTSATATGGRDGRTQVDDGSIGFDLVGFQNESGKVGTNP
                     EQLFAMGYAACFDSAMNHVAPTLGIKPTKSSTTVAVGIGQKADGAFGLDLDITITVEG
                     ISAEEAKTLISKAHEVCPYSNATRGNVDVRLHVKVVSEFDL"
     gene            23874..24725
                     /locus_tag="PWY43_000215"
     CDS             23874..24725
                     /locus_tag="PWY43_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2135134.1"
                     /translation="MQTHRAIIESIFALAPKNELWATPISGLTVHAADRSIRFNGYIQ
                     DPSLCVVLLGERQICTNGHCQVFGNQEMMFCPVNLPIVTQTLSVAENQPYLAISLRLD
                     FDLIAKIITQLPPQKISENINQRTKWELENEIKDCLLRLLNLLNSPNHIHFLAPLILN
                     ELYFYLLQSEQGGYIRSLLSQDGNIAKIANATKILERKFADTLNMSALAEEVGMSVAG
                     FYAHFKSVTSMTPLQYQKSLRLNAAREKLQAGVSVSETAYQVGYESPSQFSREYKRQF
                     GESPKRR"
     gene            complement(24801..25520)
                     /gene="deoD"
                     /locus_tag="PWY43_000216"
     CDS             complement(24801..25520)
                     /gene="deoD"
                     /locus_tag="PWY43_000216"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2135135.1"
                     /translation="MATPHINAPEGAFADVVIMPGDPLRAKYIAETFLEDAKEVTNVR
                     NMLGYTGTYKGRRISVMGHGMGIPSCSIYAKELITEYGVKKIIRVGSCGAVRNDVKVR
                     DIVIGVGACTDSKVNRIRFRDNDFAAIADFDMTLAAHQAAKAKGIEARFGNLFSADLF
                     YSPDVEMFDVMEKYGILGVEMEAAGIYAVAAEYGAKALAICTVSDHIRTGEQTTSEER
                     QLTFNGMIEIALESVLIGDKQ"
     gene            complement(25541..26317)
                     /gene="deoC"
                     /locus_tag="PWY43_000217"
     CDS             complement(25541..26317)
                     /gene="deoC"
                     /locus_tag="PWY43_000217"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2135136.1"
                     /translation="MNLKKSAEIALSLMDLTTLNDNDTDEKVIALCQQANTKFGTPAA
                     ICIYPRFIPIARKALKQQGIEQVKIATVTNFPHGNDDIEIAVAETKAAVAYGADEVDV
                     VFPYKALIAGNEQIGFDLVKQCKAVCAANNVLLKVIIETGKLKSAELIRKASEISIQA
                     GADFIKTSTGKVAVNATLESARIMLETIRDLGVADRVGFKAAGGVKTSEEAAQYLALA
                     ESILGNDWVSASHFRFGASSLLANLLATLNDEQQQAVQGY"
     gene            complement(26462..27721)
                     /locus_tag="PWY43_000218"
     CDS             complement(26462..27721)
                     /locus_tag="PWY43_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL2135137.1"
                     /translation="MGTLNSFLGIIVLLFIAFLFSSNKRAINVRTVLGALALQVAIGA
                     LVLYVPAGRDALNAMATGVSKVISYGNEGISFLFGGLVSDKMFEVFGGGFVFAFRVLP
                     VIVFFSALISLLYYIGVMQWVIRILGGLLQKALGTSKAESMSAAANIFVGQTEAPLVV
                     KPYISKMTESELFAIMAGGLASIAGSVMAGYAGMGVPLTYLIAASFMAAPAGLLFAKI
                     MVPQTEKFRDELEAVDLEKPANILDAAASGASSGMQLALNVGAMLIAFVALIALVNGI
                     LGGIGGWFGYDTLNLGQIFGWVFKPLAWVIGVPWEEAEIAGQMIGLKLAVNEFVGYLE
                     FAKYLAPESTVHLTDKTVAIITFALCGFANFSSIAILIGGIGAMAPSRRGDIARLGIK
                     AVIAGSLANLMSATIAGLFIGLGGAVL"
     gene            27902..28408
                     /gene="msrA"
                     /locus_tag="PWY43_000219"
     CDS             27902..28408
                     /gene="msrA"
                     /locus_tag="PWY43_000219"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2135138.1"
                     /translation="MQNIREIYLAGGCFWGVESFMQRIKGVIKAQSGYANGNTDNPSY
                     QEVCAGSGHAEVVKVTYDANQISLAKLLDYFFKVIDPVSINKQGGDIGIQYRTGIYYT
                     DPQDEPVVTAALAQLQTQFDQPLAVENQMLEQYFPAEEYHQEYLVKNPNGYCHIDIQL
                     MNEILKNQ"
     gene            complement(28445..29245)
                     /locus_tag="PWY43_000220"
     CDS             complement(28445..29245)
                     /locus_tag="PWY43_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2135139.1"
                     /translation="MKFSVLMSLYIKEQPQYLRECFESLKVQTVQADEIVVCFDGTVT
                     PELEAIVEEYSAILPINAVKFPQNRGLGKTLNDGLTYCKNEWVFRMDTDDICMPERFE
                     KQVAFIEQNPETVIFGGQIAEFGQDIHDLVAYRNVPTMVEEIVRFTRLRCPFNHMTVA
                     YQKSKVLEVGGYQDLQEDYYLWIKLIAQFKQVANLPDLLVYARVGNGMVGRRRGMAQA
                     EAEWRLYKLKNRCQIHDPISGFGIFIMRAIPRLLPVSALKKVYGLLRK"
     gene            complement(29245..30213)
                     /locus_tag="PWY43_000221"
     CDS             complement(29245..30213)
                     /locus_tag="PWY43_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2135140.1"
                     /translation="MFSIIVPSYNRNQEINALLESLKQQTAYNFEVIIVDDCSKIPVS
                     ETLGILNEFNDISTKEKVRELLTRDSLSHIYSYPFDVKVVRNNPNVGAAQSRNVGATN
                     ATQEWLLFLDDDDRFMPEKCERLAAEIANNPTANFVYHPAKCEMVNEGFTYVTKPFKH
                     KSELTKENILKANKVGGMPMIGLKKSLFCKIGGLSDRLRSLEDYDFLLKLLEEPSFEP
                     LYVDEALTYCTFHTKRSSVSTDTTNTEKAIEYIQQTYIKTPEQANNFKLNSLYILSYP
                     HVMNLSRKAAGYYFEMFKQTKNIKQLIIALVIFISPKLAINLKRFM"
     gene            complement(30206..31393)
                     /locus_tag="PWY43_000222"
     CDS             complement(30206..31393)
                     /locus_tag="PWY43_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135141.1"
                     /translation="MSALINLFYLYDPWFFHFLRMSFVVGLVACGYLVFQIIKGKSNG
                     IIIPKDSLIAIIALIGFSIIPLLINSTKEIGVLAMYVKMLILFVFGIAIYNVFYHNQQ
                     GKTLFVRDLKIGIAIQWICGVTALLGLPFMVDFLLSSNAMMPRFFGSEQEYRLYNITS
                     SAFFQLSIFYLMLLHFLLAYNKKTDVVNEWFLFLLLCVGLISGRTFLLMSVISIALYF
                     KWKYILPLIAFTALILFLAIVFPENRYVEHALEPVINLLSGTGKVSSSTDNLVKNHLF
                     LPTLNQFISGDGLYYTETGGYYGGSDSGYIRQVLYGGVSYMLACFAFTYYFVRKVAVN
                     WFEGSWIFTLSTIGILAILNIKADTFAFPGLMMLFLMFLSLFGTSGRNVTFFCKNKEA
                     EYV"
     gene            complement(31404..32189)
                     /locus_tag="PWY43_000223"
     CDS             complement(31404..32189)
                     /locus_tag="PWY43_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL2135142.1"
                     /translation="MKKYLISLEKDVKRRELFFSQPNTEDFEVFSAINTMSSDWEALN
                     NHFDLALFEQRYGRKTTKGEVGCTLSHLAVYQKIAEDRTIAEDDYCLVCEDDALFADD
                     FQKHLDALLAQNLKVDIILVGQSKIPTFNDSELELNYPSTFGFLQKNIPNSQYKYAYS
                     YLNYFAGTVAYLIKKSTACQFLAQCAGKNPYWLADDFILFEKNFKFDVLIVRPLMAIE
                     NPVLISNLEDLRGSLNNNLFKKAVKYPLKKLLAIKRNFTQRNS"
     gene            complement(32191..33129)
                     /locus_tag="PWY43_000224"
     CDS             complement(32191..33129)
                     /locus_tag="PWY43_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 52 protein"
                     /protein_id="HDL2135143.1"
                     /translation="MNLIICCTPLQVLIAEKIIEMHPNERFYGVMLSTVKNAKFDFYQ
                     ARLAQKCEQFFAMQQHSERIQLLKEIIYLKRTFGGKKFNKVFLANINELEIQFLLSAI
                     SFQELNTFDDGTANIAQNSIFYQAENIGLNRKLINFFLGNKYSLEKLKQRSKTHYTIY
                     KNFPNIIENTVYIDLINQVESVGTAPSNDDITHILLGQPIFERDDEKNIALAEKVIQQ
                     FGIDLYLPHPREKYELENVEYINTNLIFEDYIFQEFSHKKCRVYTYFSSAVINILNKS
                     NHIEVVALRVNVDNPAYIESYELLEKLGVQIVDIRE"
     gene            complement(33126..34331)
                     /locus_tag="PWY43_000225"
     CDS             complement(33126..34331)
                     /locus_tag="PWY43_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HDL2135144.1"
                     /translation="MKAVKDSVIYLIGELASKSIPFLLLPYFSRKLGVAGYGELSYYQ
                     TFAALFVIFIGLSQDGAVARYFYFYGKRSLNLVLSTGYLYTLCATLLILLGCWYFQSE
                     IIAYVAIGAMFQSFLNVQLSVRQCQKQALAYTTIQLMSAIFSAVITVAMMEYFKTDLV
                     EKRILAILMSNGLVFLIAYFLYAKRAKTKKFSLARHKTALLYLFGFGIPLILHHSSIF
                     LKGQLDRVFIYHKFSEEDLGLYAMGAQIAMILAIVVQALNKATLPYFYEALKQKKINV
                     SHIHKWALYALFIVPLPAIVMWFIPEELVVWILGSQFVGTKYYIILFLIATMLVVPYL
                     ILVNYLFFYGKNKLISLCSILSTIFYLVALFALTFTKIEYIPFASIIGAISIIPILYI
                     MTAKVGKTV"
     gene            complement(34341..35525)
                     /gene="neuC"
                     /locus_tag="PWY43_000226"
     CDS             complement(34341..35525)
                     /gene="neuC"
                     /locus_tag="PWY43_000226"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL2135145.1"
                     /translation="MKNIAYITGSRAEYGIVKRLLQKLAQDPTVNFSLVVTAMHLDPQ
                     YGNTVTVIGQDGFEIAARIPLTLNSENNQTIIHSMAECLDRFGEHFQQHRYDAVIVLG
                     DRYEMLAVATAAAMHNIPLIHLHGGEQTLGNYDEFIRHCITKMSKLHLTSTEEYRQRV
                     IQMGELPENVVNTGSLGAENSLDLVLPTQAELVERLGIPASPYFLVVFHPETLTDVSV
                     LEQIEALLNAFEQFKDKYQFVFIGSNSDTNSEQIYARFKAYTDENQFKFFTSVKPEEY
                     LALIKYSNGLIGNSSSGLIEAPSCGVGTLNIGNRQQGRVRGESVIDVESNEQAIIDGI
                     EKLISADFQHRLPSMKNPYFQDNAMEKAYNAIKIFLADSHHTQPKGFYDVAFSQAVKK
                     SE"
     gene            complement(35526..36785)
                     /locus_tag="PWY43_000227"
     CDS             complement(35526..36785)
                     /locus_tag="PWY43_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL2135146.1"
                     /translation="MKKIAIITARAGSKGLPNKNVLLANGKPLMAYSIEAAIESGQFE
                     KIIVSTDSQEYIDLLSHYPIEFIKRAEHLASDKASTFVVIEDVLNQYRSVEFDYFMLL
                     QPTSPLRSGEHIKESCEKFEKNFEQFDFLVSVSDAHKPTTLTRMIEEDESLKHFQLDY
                     SNYARQLHNPEYSPNGAIFTAKPEAYLKQRHFYGAKCLAYFMDKDVSIDIDDRQDFEY
                     FYFILQQRNKEKILLETIKRSILMKKENFKEVKEIALIGHSIIEYWDIEHLNGKAVNN
                     LGIAGISTKQYSDLILDEGLISALPKDIILMFGTNDIVYAGWSDEKILADINHLIAQL
                     TAIRGDINLYFLEITLTAFRVDRNNNDIRLLNQYLKDNLAVKWISLDQAFSDKYGKLD
                     LSYSDDGLHLNAKGYEKLTAILEKELN"
     gene            complement(36845..37882)
                     /gene="neuB"
                     /locus_tag="PWY43_000228"
     CDS             complement(36845..37882)
                     /gene="neuB"
                     /locus_tag="PWY43_000228"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL2135147.1"
                     /translation="MVNVFIVAEIGCNHNGDPALAKKMVDVAKECGVDAVKFQTFKAD
                     KLISKYAPKAEYQKVTTGTADSQLEMTRKLELPYEEFVKLEAYARSLGLEVFSTPFDF
                     ESIDFLASQQQKVWKIPSGELTNLPYLEKIARLPIEGKTIVISTGMATIEETKASLKV
                     LEDNGVAKKDITILHCNTEYPTPYEDVNLNAFHQLKQEFGDYSLGFSDHSAGYFAGLA
                     AVPYGITFIEKHFTLDKNFEGPDHKASVTPEELKLLCEGIRAVEKSLGSSEKLVTNSE
                     AKNKIVARKSIIAARPIKKGEIFTTENITTKRPGNGISPMFWYDVLGKEAQQDFEEDQ
                     LIQDSRFENQM"
     gene            complement(37882..38526)
                     /locus_tag="PWY43_000229"
     CDS             complement(37882..38526)
                     /locus_tag="PWY43_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2135148.1"
                     /translation="MQQKSKREKLILIGAGGYAKSVLDSLDHEQYEFCGFIDNFKPEG
                     SVHLGYPILASTIDCFSKRDNYHYFICIGNNSYRLEKFSKLQKYKCRIINVVDKTALV
                     SKNSTLGIGVFVGKMAIVNSGVTVGDNVIINTKSLVEHGCFIGSHCNISTNTTLNGDV
                     IVEDHAFIGSSSVVNGQLRVGESALVGSGAVVIRNVEPRTVVAGVPAKYIKDIK"
     gene            38659..39402
                     /locus_tag="PWY43_000230"
     CDS             38659..39402
                     /locus_tag="PWY43_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2135149.1"
                     /translation="MFEFIKLITAMILPPFNIILLWLLALILFSLGYKYAAYFFALLG
                     ILILYIFSIPYTSQKLGDSLVEAEPFTIEKYKNAQAIVVLGGGLRDSQELFGKLAITG
                     IPLERMRYAAYLHKETGLPILATGSSPNGTSEAKVMADEFKMFFNIETKWLENKAKTT
                     KENAIFSREILEKENIHKIILVTNQWHMKRAEMLFSQQGFEVMSASVGSGDTPDHYKL
                     NFMHFIPQAGAMNSNMLALKEWIGYWKEK"
     gene            complement(39479..>41344)
                     /gene="priA"
                     /locus_tag="PWY43_000231"
     CDS             complement(39479..>41344)
                     /gene="priA"
                     /locus_tag="PWY43_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2135150.1"
                     /translation="FVVTEQGRKSLIIGENKKAKKQVELLTELAECAKYFEKPTACSP
                     SAWKGLLEKGYIEKIDVPFMAQAWQDSLGEFPLVNTNNRLILNKQQTLVVSRLNAQTT
                     FASFLLNGVTGSGKTEVYLQLIETVLKREEQVLLLVPEIGLTPQTVQRFKARFNVEID
                     VLHSNMNETERLNAWLRAKNGESAIVIGTRSALFTQFQKLGVIILDEEHDSSFKQQDG
                     WRYHARDLAVVRAKNANIPIVLGSATPSLESLHNVQNGKFIELTLTARAGNAQLANQQ
                     IIDLKTQRVTAGLSERLLSMMKTQLEQGNQVMLFLNRRGFAPVLLCHECGWVCECEAC
                     NKPFTYHQKQKVLRCHHCATQRVIPRQCGHCGSTHLITTGVGTEQLEQVLNQQFPHYQ
                     VTRIDRDSTARKGALENHLTAIREGKSQILIGTQMLAKGHHFPNVTLVAIVNVDSALF
                     STDFRAEEKLAQLYIQVAGRAGRAEKKGEVVLQTHYPDHPLLKTLLEQGYRAFADEAL
                     KMRQLMSLPPFSSQVLFKATGKDNQQILQLLEQLADYFSQKIQQQNGQGFQLPPPFSA
                     PMAKKAGHYRWLLLIQHPLRGALQQLLDAFDQERESLNIPNNIRLNIDIDPQEIS"
BASE COUNT        12456 a         8888 c         7625 g        12375 t
ORIGIN      
        1 ccttcgccga attgataaat cagtacgtta tctaagcttt tttcattttt aatatgattg
       61 atatagataa atgaattgcc atctttcgcc caaaacccgt cttttgtcgc aagcattgag
      121 ccaccgcttt gagcaatcga acgcatatta cgggcaaatt gctcggtttg tggcacgccc
      181 cattcgccaa taatcatagt aaaaagtacc agcggtaagg cagttttcat cacggctaaa
      241 ccaatgcgga aacgggaaaa accggaggct tgcatcacca ccaattcact gcggctcgca
      301 aggttgccta aaccgagtaa tgagccaagt aatgccgcaa tcgggaaaaa ggtttcgata
      361 tctcgtggaa tagttaaaaa agtatagtat gccgctttta agccatcgta agagccacgc
      421 cctacatcac gaaactcttc cacaaattta ataatcgccc caagcccaat caacataaac
      481 agggttaaaa aaatcgcgga taaaatcgtt ttgccgatgt agcgttctaa aatattcatt
      541 gagaaaaatt gcattatgcc accgccttat tggttaattt ttttgcacca aattgatagc
      601 gtaacttaga cataaattta ctatcccacg catttaaaat aacacctaaa actaaaaagg
      661 caactgatac caacggcatt aacacttccg cattcaactt gcctgatgcc cctgacgatt
      721 tcagcgagct ttgtagtaag aaatagatta aataaagcaa taacgccggc agtagtttag
      781 caaaacgccc ttgacgaggg tttacactac tcattggcac cgctaataat gccattaacg
      841 gtacagctaa aatcaaagca agccgccaat gaagctcggc ttttgcttcc ggcgttttgg
      901 tttccattaa tttcttgaaa tcggctcgtt gcactaattt ctcatcagat tcaacgtctt
      961 gatagcctaa ataagcagaa taattctcaa aatgtgaaat acggaaatcg gcactttgtg
     1021 gtgtaccttc aaaacgcttg ctctctgcca aggttaagac ttgatcacca tttggcaaac
     1081 cttgcaacgt gccggattca gcaacaacca ctgaaggttt gtttttcttc tgctgttccg
     1141 gctggaaaac gtagatatca tttagactgt tattttcaat tttatcaata aataacacat
     1201 agccaccggc agacataaat tgtccggccg aaagagccgc aaaacgtggg ttggctttcg
     1261 cttccgctaa catttcactc tgtttattaa ttgcccaagg cgtaagccaa aacacgttat
     1321 aaaccgctaa tgcagtggta aaaatcgaga gaaacatcgc cactttaacc aatagccctt
     1381 gcccgatacc acaagcccgc attacggtaa tttcactttc ggcataaaac cgcccgaggg
     1441 ttaataacaa ggcgataaac agtgagagtg gcagcattaa ttgtgccatt gtcggcatac
     1501 ctaagcctaa taagctcacc actaaatctg ccggtacttt accactcaca gcagaactta
     1561 gaacacgcac taattgctga caaaaaaaga tcagtaataa gataaacaat atcgccaatt
     1621 ggcttttaaa tatctctttg gttaaatatc ggcttaagat cacgtttttt ctctataaca
     1681 tttacaaatc tgaataccgc taaacacaaa cggtctattt ttgttaattt tttgcaaaga
     1741 gcgcaaaata acttattcga atcgctaata ataccttttt catacaaaaa aatcacctat
     1801 agaaaatatc acgcacggca aaagcgtgcg taagtattat tgttttttcg attttttcga
     1861 tgatgttgct ttatgagctt caagaattaa tttcttacct tggctatctt caatttcaat
     1921 cttaggtggt gcttccgtat tttgatgaag gatagctata atattttcac cttgtgcaat
     1981 atcttcaaag tttcctttca cacgtaactt agccgcatca acactagtgt caaatactaa
     2041 gaatgtacct aatgcggtaa ctctaagatt tttaagccct atattgtcct tcgttaccgt
     2101 tttatattga ggtgcttttt tcccttttgc agccataatc ttctcttgat tagtcgcaat
     2161 attaagaata ttttgaactt tagtcgcttc tgctgtagat tgtgcctgtt ctgttaaatt
     2221 tggttctcca tcaaaaataa catccccaac aaattgcacc tctttaggct cattttgcaa
     2281 taatggtgca acgtctttag ctgaaacaac caaaaataac cgttcaccta ttttttcaaa
     2341 acggacatca atattggtta attgtgattc aatttgttca ttaattgtct tgatcgcagt
     2401 ttctgtctca ttttgaagcg atattttacg tgtaatatca acatcataag caataaaacc
     2461 aacttttaaa cgttgtgata actgctgaag taaattgccc acatttccat cataagaaag
     2521 acgtaacttt gcctgctcat agcgactaat ttgagcatca tcattagcga aaatagtgtt
     2581 ttgagtccct aagccgatta gaccaacaac aaacgtttta ccccaaaagc gcatcatttt
     2641 tatctccaat tattctctta aatctaaaaa actcttttag tataaagtaa gcaaactaaa
     2701 agaaaaacca gtaaaattat ctatacgacc gcaaaaaaca tttaatattt gcaaataaaa
     2761 agtaaattat tttaaataat gatttacatc agggtatatc tatattttac ttatcattga
     2821 tgattatgtt ggcattataa aatctgtagc aataaaaatt aaggaggaaa aatggaattt
     2881 agtgtcaaaa atggcagtgt tgagaaacaa agaaccgcct gtttggttgt tggtgtctat
     2941 gaaccccgcc gattatgttc tgccgcagaa cagcttgata aattaagtaa cggctatatc
     3001 agcacattac tgagaagagg agatttagaa ggcaaagcag ggcaaacctt actcttgcat
     3061 aatgtgccga atgtactcgc tgatcgagta ttattagtcg gctgtggaaa agagcgtgaa
     3121 ttaactgaac gccaatacaa acaaattatt caaaaaagtg tacaaactat taatgaaacc
     3181 ggctcaatgg aagctgtcag ctttttaacc gaactgcacg ttaaaggtag aacaccttat
     3241 tggaatgtgc gatttgcggt agaggcaatc caagaacaac tatatgccta taacgatttc
     3301 aaaagcatca aacctgaagt tcgccgtgaa ttacgccgtg tgatttttaa tgtcgcacac
     3361 cgtaaagatc ttcctgatgc cgaacgagct ttagaacacg gaaaagccgt agcacttgga
     3421 atcagccacg ctaaaaatgt agcaaattgt ccgcctaatg tgtgtaatcc tcactattta
     3481 gccgaattgg ctaagggttt agcctacgac tatcctgcca tttcaactac cattattgat
     3541 gaaaaacaga tggcagaatt gggaatgaat gcttacttag cggtttccag agcctctgaa
     3601 aatgaggcct ttctttcagt gattgaatat aaaggcaacc cgaatgctga tgcgaagccg
     3661 attatcttag tcggcaaagg aatgaccttt gactctggcg gtttatccat taaacctgcg
     3721 gaggcgatgg acgaaatgaa atacgatatg tgcggagctg ctactgttta cggtacaatc
     3781 aaagcaattg ccgagatgaa attaccactc aatgttatcg gcttactcgc cggctgtgaa
     3841 aatgcggttg atggcaaagc ctatcgaccg ggggatattc tcactacaat gtctggttta
     3901 accgttgagg tactcaacac cgatgcagaa ggacgtttag tgctatgtga tgctctcagc
     3961 tatgtagaac gttttgaacc ggaattagtg attgatattg ctaccctaac tggtgcttgc
     4021 atgattgcct taggtaatca taacagcggg ctgatgtcac ctcataacgg tttagcaaac
     4081 gaactcttag ctgccgcaga gcaagccgat gacaaagcgt ggcgtttacc tttaggggaa
     4141 gaatatcaag aacagttaaa atctaacttt gctgatcttg ctaatatcgg cggacgttta
     4201 ggcggagcct gtactgccgg tcaattcctc tctaacttta caaagaaata tacttgggcg
     4261 catctagata ttgccggcac ggcttggaaa tctggggcag ccaaaggggc gaccggcaga
     4321 ccggtatcct tactaacaca atttttgatc aataaagccc aggtctaacc ctcacaagcg
     4381 gtctgatttt atcgagtttt tgcaaaaaga aaatcagacc gatcctcttc atacgaccct
     4441 atattctttt tagttatatc aaataaattc cagctactag aaaagcgatt aaaaataagt
     4501 atgataaatc ccaatctcaa tacttattaa ggaatatcac tatgactatc tatgcagaca
     4561 actcatactc aatcggcaat accccgctgg tgcgtttaaa gaattttggc aacggcaata
     4621 ttgtggtaaa agtggaaggg cgtaacccaa gttttagcgt gaaatgccgt attggtgcga
     4681 atatggtgtg gcaagcagag aaagacggta ttctaaccaa agacaaagaa attgtagatg
     4741 caaccagcgg taacactgga attgccttgg cttatgtagc agccgctcgt ggttataaat
     4801 taacgctcac aatgccggaa acaatgagta ccgagcgtaa acgcttattg cgtggcttag
     4861 gagtaaattt agtcttaacc gaaggggcaa aaggaatgaa aggagcgatt gcgaaagctg
     4921 aagaaatcgc gtcttccgac ccagagcgct atgttacctt aaaacagttt gaaaacccgg
     4981 caaacccggc aattcaccaa caaactaccg gtcctgaaat ttgggatgca actgaaggta
     5041 aaattgatgt attagtggca ggcgtaggaa caggtggcac tattaccggt gtttcccgtg
     5101 ctattaagca agataaaggc aagcaaatta tttcagtggc ggtagaaccg agtgaatcgc
     5161 cggtcattac gcaaacttta aacggtgaag aagtcaaacc gggcccgcac aaaattcaag
     5221 gcattggtgc aggttttatt ccgaaaaact tagatttatc actgcttgac cgtgttgaac
     5281 aagtttcaag cgaagaagcg attacaaccg cacgccgttt aatggcagaa gaaggcattt
     5341 taggcggtat ttcatcaggt gctgccgttg ctgccgcaga tcgcatcgct aaactacctg
     5401 aatttgccga taaattaatt gtagtgattc tgccttcagc ctcagagcgt tatttaagta
     5461 cgctactgtt tgaagggatt gaagcgtaat attaaattaa tataaaaatg ccggctgatg
     5521 tcggcatttt tattgcaaac aagcggttat tttttataaa aaatttgcaa tttgtctcct
     5581 attgcgttat tcttcgccac atctttacgg ttggccgtat ctaatcaata tcaacaaatc
     5641 caatcttaat taatcacaat tttacattca aattatgcat cttactcaac ttaaaaacac
     5701 ccctgtttca gagcttgtgg aaatgggtga aaatcaaatg ggcttagaaa atctggctcg
     5761 tttacgtaaa caagacatcg tatttgctat tcttaaacaa cacgcaaaaa gcggtgaaga
     5821 cattttcggg caaggcgtgc ttgaaattct accggacggc ttcggtttcc tccgctcatc
     5881 agatagctca tatttagccg gccctgatga tatttatgtt tctccaagcc aaatccgccg
     5941 ttttaactta caaactggcg ataaaataga aggtaaaatt cgcccaccaa aagagggaga
     6001 acgctatttt gccctactga aagttgattc agttaatgat gacaaaccgg aagcctctcg
     6061 cagtaaaatt ctctttgaaa acttaacccc tctgcacgcc aattctcgct taaaaatgga
     6121 gcgtggcaat ggatcaaaag aagatttaac cgctcgtatt ttagatttag ccgccccaat
     6181 cggtaaaggt cagcgagcgt taattgttgc cccgccaaaa gctggtaaaa ccgtgttact
     6241 gcaaaatatt gcacaaagca tcacccataa tcatcctgaa tgtgagctta tcgtattatt
     6301 aattgatgag cgtccggaag aagtaacaga gatgcaacgc accgtacgtg gtgaagtaat
     6361 tgcctcaacc tttgatgaac cggcaacccg ccacgttcaa gttgcggaga tggtaatcga
     6421 gaaagcaaaa cgctcggtag agcataaaaa agatgtggtg attttattag actccatcac
     6481 tcgtttagcc cgtgcctaca acaccattac cccggtttcc ggcaaaatcc tttccggtgg
     6541 tgtggacgct aacgccttac atcgtccaaa acgtttcttc ggtgcagccc gtaatgtgga
     6601 agaaggtggc agcttaacca ttatcgcaac cgcacttgtg gataccggat caaaaatgga
     6661 cgaagtgatt ttcgaggagt tcaaaggtac gggtaatatg gaattacacc tctcacgccg
     6721 tattgctgaa cgccgtatct tcccggctat tgacttcaac cgctcaggta ctcgtaaaga
     6781 tgacctctta atggcagcag acgaactcca aaaaacttgg atgttacgca aagtcctcaa
     6841 cccaatggac gaagtagatg ctatcgaatg gctaatcgac aaattaatga tggcaaaaac
     6901 taacgaagag tttttcgaga ttatgaaacg ttcgtagaac ataaaaaaat cccgcttaac
     6961 gcgggatttt tatcaatagc agctgtcaat aaagtaacta cttaaaatcc cccaactcaa
     7021 acaaccccat tgatctcgcc acatttacat aagtataaag tgcctcgatt ttggcttttc
     7081 ccggtgttgc cggcttgcca ccaccaacgg aatccatctc aatcggataa ccaatgtgct
     7141 gtgcataacc tttgagaacc gctgttgcac gaggaatatg ttgtaacgta ccgataccta
     7201 atactttctt gccattatag gtttgcatca gcttgataat cccaaccgca tttcccgggg
     7261 tatctctggc aacaggatca agtagcaaac ggtctttttc aataccattt tcgctaagcc
     7321 attttgccat cacatcagcc tcagcaaatt ccgttttcac agcaccgcca ctgacgataa
     7381 tttttgcttg cggaaatttt tgtgccatct ctttggtttt ctgtaatcga tctaacaaca
     7441 gcggcgaagg tgtgccgtct ttgtttggcg tagaaccaaa aacggcaatc gtatcaggga
     7501 tcattgctgt ctgtggttca ttaccgtagc ttgccgccag cgtttgttcg gtaaaggcaa
     7561 aaaagtctgt cattcgttgt gcgacttcgg gagcaagctg tttcagtgca gaaagacgag
     7621 ccgtttcttg ttcagtatct tctagcaccc ttgaagctaa tactgtcatc gcaagagcgt
     7681 gaacatcacg ggggttttgg cgcaaaattt ggtcgagctg agaaagtgct tcccgatact
     7741 ctttattgac gaaatacgcc tctgcttgta aacggtaagt gttaaggtag gtcggcttct
     7801 ctttaacttt ctgatcaagc gaagcataca tcgcttgcca ataccaacgg cggaaattca
     7861 gcacatcctc ttttagataa gggtcatcaa catagtctgg cgcatattga ataactaaat
     7921 gctcaatcgg cgctcctcga ccatcttctt taccttttgg tgcaggcagt acttttgcta
     7981 atacaaagtt aaacgcctta tcatattgac tgatattgtc ttggctcaaa taatcagctg
     8041 gtatcgccca gctatttaca cttatcaaag aaaggcttaa agcaagggca gaaagggttg
     8101 ttttgatttt tttcatagag gctcctattt accattgata gtgataattt gcgtgtaatc
     8161 cgtatgaacg cttcaatgca tccccgtgta acattgatcc ttcgattgca aaataatggc
     8221 gatttccaac tgcttgtacc ccaatactga ttacattccg atgacgatca gataaactat
     8281 ctaaggtatc aatatgacga tttcgaaaac gatagctaat atcagcataa ggtttaagtt
     8341 tgattgcatt gagttcaatc acttttccta aacgtagccc tgtcccagcc caaaacattc
     8401 gtgctgatcc agcttttgcc cagcctaatt gcagctttgg cgtaatttgc cattgatgac
     8461 tcatcacgat atttcttcca atttcactac tcagactgta tgcattatta tcacgttttg
     8521 ttacaccaga ttgattagta aaatgactgt aattaaaaaa atttgtccaa aacagaccac
     8581 ttgtaggacg ataaccagca taaataccag cagatgtccc tttcacttta tgcgtcctat
     8641 tctcaatttg cccctgactg ataccaaata ctccaccaat agaccaatta tgggaaacct
     8701 gatgatctag cccgatatgt aataagtgat agtcttcggt tgaacgattt tttaaacgat
     8761 atttggatag actatccgaa agccataaat tccaatcttg tgtatgttct atttctgcta
     8821 acctacggta cagatcataa gttgcttgtt ccatcaaatt aagctgtaca acataatgct
     8881 cggaaaaagg attgttctgc tttattatta aacgactaag ggcttccgta ctctgtttta
     8941 actgctcttg cgttttacta agttctttat tcaattcctc ttcagcttgc tgatgagcct
     9001 gttttgctgt ttctaacgcc tgtctggcta cttccagctc atttgcagaa ttttctgcat
     9061 tttttactgc ttgatctcgc tcagctacca atttttctaa ataattctgc tgtgcttttt
     9121 ccaacgcttc tctttgttct ttttcagctt gttctttggc aagcaaattc tcaaaatcgc
     9181 ccaaggtaaa taaccccatt gcacgagcaa cattaacaaa tgtataaaga gcttcacctt
     9241 ttttattttt ttcattagga ggagaaccgc ctccggcaga atcaatttcc aattcatatc
     9301 caatggaatc cgcatagctt ttcaatacag acattgctct cggtaaatgt aagatcgtac
     9361 cgatccccaa tacttttcta cctccatatt ccttcataaa atcaatgata ccgacagcat
     9421 ttcccggagt atctcgtgcc acatcatcaa gcaacagtct gctttcatca acgccattct
     9481 ctatcaacca ttttttcatt acatccgctt ctgcatagat ataacgaaca ggtccaccac
     9541 ttaaaataat ttttgcattc ggaaatttcg ctgccatttc tttggttttc tcaagacgtt
     9601 gcaataatcc cttgcttgga gtaccgtctg ggttaggcga ttgcccaaat accgctatgg
     9661 tgtcggggat cattgttgtt tgaggctcac taccatagct ggcagcaagc atagtgtctg
     9721 taaaatgtag atattgctgc accttttgcg caatacttgg agagatttga taaagcgcta
     9781 ataaacgctc ggcttcagta ttaggatttt ccaatacccg acttgcaacc acactcattg
     9841 ccattgcatg aacatcatta gggttacgcc gcagaatttg atccagttga gataatgctt
     9901 cttggtattg cttattgata aagtacgctt ccgcctgtaa tcggtaagta tttaaataat
     9961 cgggcttttg tttcgttttt aaatccaatg acgtatagag atcttgccac tgccaacggc
    10021 gaaaacgaat aacatcctct ttaagataga tatcattaag ataatctgat gcatatttca
    10081 ccaccaaatt ctcaatcgct tcaccacgcc catctgcttt tgcaataggc gcaggtagtg
    10141 gatttgcgag gaaaaaatta aaacgggcat caaattgccg aatattatcg ttacttaaat
    10201 aaccgtcagc ataagccgat aaaggtaagg ctgaacctaa tatcaaagca agataagaat
    10261 aacgcaagcg tttcattatg atttctcctt atattgggtg aagaagtatt tataactttt
    10321 tacatctaac caacaaagtt gattaaggat aaaatttgtt tgtgctattt tattaataat
    10381 attcaaaatc atttttaatt tttgtggttg ctcaatgaca ttaagactca tcacaataaa
    10441 tttagtataa ccatataaag tgaaatcttc tttattttta aaataataga tctcaaattt
    10501 ttctaccgag gcattgagtt gataaaatag tttaatcttg tcttgtttat tttctaaaat
    10561 aatctgttcg taattaagat attcttgctc aagatgaaac tcatttaggc gatattgtgc
    10621 ttcaatttca atcgatttaa tgctaggcaa cacctcatca tattgataag cgtgataaaa
    10681 tacttcgttg agtgatgttg catattcatt ttcatttaaa ctactgtcag caaaataaat
    10741 aggcttatag ggaatagcca tattttttcc atattcatta cagagatagt agtcataagg
    10801 tttatcttcc attatggcaa gctcatatag cactttggtt tcaattttca aaatatagtc
    10861 ggaaaagtac cgcttaatat tccgttttaa ctgttgggtg cttaatctgc cattaagggc
    10921 aataatcgcc agtcggagcg gtttacgttc taattcaatt cgattaacca ttccggaaaa
    10981 aatatcaaaa ataaaatact gttctcgaat agataggcta tacccgtagt ttttttcaat
    11041 gtcatccact aatttttcta ctgtatgagc caaaattgga tttctatgta gttctgtttg
    11101 gttaaatagc cctaaatcca catcatctga aatgctaagc atcattttca ttgcgatagg
    11161 gatcagcatt ttaagcaaat ctttacggca agtgtcatcg tgaatttccg ataaatttaa
    11221 atatgcagaa aactgagtaa aaataaaacg atgaatttcc caagccttat ctagcaacaa
    11281 tgggtaatta tccgaattac aatctttaaa gcggtagaga tctgtactgg ctatcgcaat
    11341 ataggtcaga aatcgaataa cttccggatt aaactcaaaa cgactgtctt gttcacgaag
    11401 ctgttccagt aacttaatta caaactgata ttcattcgtc tgttgataat taaaatttaa
    11461 cttcggtaac aataactcag gctgttgatt ataaaaatag cgtaagtaaa taataaaccg
    11521 ctcagcataa atatcagaaa atactaaatt gaaatgaata attgtcttag ctaacctttc
    11581 acggattttt tcagtcatag gacaagcaaa acactgttca aattctttaa ttccaaatgg
    11641 atgttcaagc tgggtaaaat atttatataa tcgtactgtg agcattattt tacgataact
    11701 ttcacctaca atgttcattc cgtgataggg tttcgcttcg agtatcaatc catattttcc
    11761 taacaaggct ttaacttgac taaattcttt tgttaaggca ctggtaaaac ttaaataaag
    11821 ctcactctga aaatcttcaa ctttcaatgc acgctggctc accagtaatc ggcgtaaaat
    11881 cgcatttacc cgagactcaa atttattatt taccgtcatc ggctgtgata tttggaaata
    11941 ctgttttagt tgcgtgtgta attcttcaaa ctgaatcaat aatttatagc cataaaacgg
    12001 tcgagaaaaa atctgaatgc cgtaaggttc aagtttttct tttaataact taatctcttt
    12061 ttttaccgtt cgtactgaaa gctgtaactc cgtagaaagt gcatcactat taataaattg
    12121 ttcacttctt aacagtaata aaaataatga ttgaaatcga cggttacggc ttaatagtgc
    12181 caatatcttt tttaaatcgg cttgaatatc ttgcgacata atattccctt atgtgtgtca
    12241 gaattaaata ggatttcgtc agccctttca attttaaggc ttgcacgata tgtagtgaat
    12301 taagatatga taccgaattt atcactagaa gtcctaaatt aggtttaatt ggagaaaaag
    12361 aatgaagcgt agaaaggtgt acttttatat acaacaaaaa agctgccttt cagcagcttt
    12421 ttggggatag tagcattact agtgctgtaa acaacatctt acagggagca gctggcaact
    12481 gtcccgactt ttttatgcta cctttttcgc tcactttgtc aaaatctcac aatgtatcag
    12541 caatgacttt ttccagtaac tgtttaaatt ccatataatc gcataatcca tattggtcag
    12601 cttggcaata atccaaactc aaaatgtgat gttgcggtgg tttatcaagg cttagtggaa
    12661 tcgctttacg aatttggtca gtagattgat atacatattc cagtttaact ttttgtgctt
    12721 ggctactttt ttcttttcag atttcaataa agactttccc tcctattgaa gtttgttcat
    12781 attgatgtgc taatttataa ggcttaacat ttaatgcaga aagtaaggct ataatattgg
    12841 aatcgtgtcc gactaaaagt gttattttat tttcactatt taaatcttgc tgaatatatt
    12901 ttaatagcgg taatgaaata tgtttagcaa taaatggatc tcctcgtagc ataaattggt
    12961 aatcgttttt aattttattg agtgtttgcc atttctgatc actatcaagc tggttatttg
    13021 caatctcttt taaaggaaga ccttcataat attgcagcaa taaggcatcg acaatatttt
    13081 cacttaatcg catcgtaccg gaaacacctg gttctttatc ctttttaatc cttaatttac
    13141 caacatcatt aaaaaattca cactgttttt cctgcaaaca tttttctgaa tgagcataat
    13201 cgattatttc gcttaataaa tgataggatg gcgctaaata ctgatgggtt ttattgaggt
    13261 caattttgct taatgctgtt tgcacaaatg tctggtcatc ggagcgaata attggattaa
    13321 acaccggatc catctttcca atctgctggt ggtgtttaac ttgtacatta caacctgaaa
    13381 acccaccggc aaccaaagct tgtgcagtgc caattgtacg ttgcaggcta ttagcataaa
    13441 cctcaatctg cttcccactt tcacagcttg ccttgtccaa taacccatta tgtgtgagcc
    13501 attgaccaag atactgccca aaataggttt ccaacagcgt ccctttatgg gttaaatgac
    13561 cggtcggtac actccaattt gcccatttat aaggggttga tttttctaaa tcactgcctt
    13621 tttctgtaag aggggaacgt aacccgtgtc ggctaaatat aatgattttt tctaattcgt
    13681 agtgggaagt tggtatattt tcaattggtt gagcaaaaag aggaaatgct gtgaaaattg
    13741 ttgtcagaca agcggtagtt ttataccaat gttttgcaaa ataacgcata tgaaatttct
    13801 ccatttgtag tgtgttcaat attacaccct accgttctac gccaaataat ttgcccgtaa
    13861 tgcttcaatt tccttcgcag taatacctaa taccctttgg gcatattgct caatagtgcc
    13921 atattgggtt tcaatagtat taagcgattg ttcgataaat tcgggttgag tatcgattaa
    13981 agacaatagg tagtccaaca catcttgatc ttgagtgaag gtacggtaaa tcgccatttt
    14041 ctcttcgtta cgagcaaggc ggttatcatg agtaagcata taatcagcaa taatatcagc
    14101 tttactcacg cctaaaatac caagcagtaa cattgcaccg aaaccagtgc gatctttccc
    14161 tccacggcag tgttgaacca atggtgcatt ttcagggtct gtcgccaacc ttaacatttc
    14221 agcaaaggcg gtttggcatt ctgctttttc gacaaaatta cgatattgtg ccattacaat
    14281 gtcgtagcga ttgataagat taccttcagc tttttgagca atgattttat tgaccaaatt
    14341 acgatcttca tcgtgcttgt ctgcactaaa ttgggcagca agctcggcag tatgtgcatt
    14401 cggatcaagc cgaaaagttt gctctctgcc attaattggg ggatttgggt atttggcgac
    14461 ttcattcggg ctgcgatagt caataacagt ctgaattccc aatttatcaa gataagccac
    14521 gccttcgtct cttaaattat gcagatgatc ggagcggtat aacttgcccc atttcactcg
    14581 cttgccctga tgagcgacat agccccccat atcccgaaaa ttattcattc cggcaacagg
    14641 taaggtgcgt tcagcaacaa tctgttctcc gtttgctgat tgaatgagga agaacggacg
    14701 ggcttcacaa gcgctcaatt tcagctcaaa ctctgccaaa tcagtctccg ccagccattt
    14761 gctatcctta ctgtcagggt tgtccagaaa gaaaatctgt accgattgag ccggctgttg
    14821 gcggaacagg tatctgccct gttccaaccg aataatgtca tacacactgt ccattagccg
    14881 agtattcctg catagactaa gccgatacag atccccatcg tagcaaggat catcgttgca
    14941 gcatatttgc cgttagtttt cttcaaaatc cagtagatca cgacagttaa aagtaagctg
    15001 agtagtcccg gcatcacttt atctaacatc tcttggaatt taatcacatt atcaccagcg
    15061 gcaatttgtg aaactacttt gactttcaca acggaggcaa ttaatgcgcc aatcaccatt
    15121 aaaccaacca catttgccat attgccgagt cgttgcaaaa tgttattacc cgcaccgtga
    15181 attaaatcaa cccctttgct gtaacctaga tggataccgt aatacttcgt cgcaatgttt
    15241 acactgttat acaacaagaa cattaaaatc gggcctaaca cattgccact tagtgccaat
    15301 gccgccccga tactaccgca aatcggtaac caggtgaatt tcaacaagct gtctcccagc
    15361 cctgctaacg gccccattaa accggtttta atcgcggtta ctgtctcttt ttcatcctct
    15421 ttggtagtct cttctaccgc agcggcaatt cctaaaatca atgcatcaga attaacgtgg
    15481 ctgttaaaaa attttaagtg gcgtttcatt gcttccgctc gttcttcctg tggacgatct
    15541 ttataaagtc gttttaatac cggaataaag ctacttaaaa atccaatcgc ctgcatcgat
    15601 tggaagttgt ttgtgacgtt cagcacctgc atccgccaaa acactttgtt taaatcttgt
    15661 ttggtaattt tattgctcat aggtcataat cctcttcatc tgcggttttg gttggtgcag
    15721 cttcagcttt tttgcttact gctaagtcgt aaatcaacgc aagcggtagt gcaattaagg
    15781 taatcggcaa tactgataat ttgagataaa ccgccaacgt aaagccagca attaagaacg
    15841 tccaaagttc ggctttctta atcatcatta acatcagcaa accgataccg atggcaggaa
    15901 taattttgcc tgcaacattt aatccggtaa tcacttttgc cggcagagca gagaccgctt
    15961 gggcaataat gtccgccccg taatagacgg ctaagaacgt tggaatagca cgaatagaaa
    16021 aggcaattaa cattgggata tagtgaatgc ggaaaatctt gtcaaaatca ccgcttgccg
    16081 cccagcgttc agcaattggg ttcaatgcac atacgccgga gcggtaaaga atgagtaact
    16141 gctgtgccaa gagtgaggtc ggtactgcga gtgcaaccgc agtagaagct tcaccagcag
    16201 caataccaag tgcggtgcca atagcggttc cggaaatcat atccggtgca gaatatgcac
    16261 caacattgcc gatccccatc cacatcactt caagcgtagc accaataatc aacgccgttt
    16321 ccaaatcccc cataatcaat ccgaccaccg gtgcgattaa caacggacgg cggatcatct
    16381 gtgtgccaat atcatctaag gcacaaatac ctgcccagca ggcaacaagt agtgcagtca
    16441 gcatagctag ataatctcct gtaagttaag tttttcatca ttcggtacca tctgtaccgt
    16501 tacttcaaca cccttttcca ataaggtttt aaatgcttga cgctcttctg gcgtaactga
    16561 aaccgcttta cttaactgct cacgcccagc ttgataacgc attccgccga cattcaattt
    16621 gtcaatcgat acacctagtt ctacagtgcg taacacatca atgctattgg taaacaacaa
    16681 catagtacgg cgggtaattt gtgtcttctg caacacttca ccaaattttt caatgccaaa
    16741 cgcgaccact ttaattccct gcggtgcagc catcgttaaa atctgttttt gggttggatc
    16801 cgctgccacc gcatcattta acacaataat ttgttcaata cggtgtaaat taacccaagt
    16861 ggtgactact tgaccgtgaa ttaaacggtc atcaatacgg ctaaagcaga tcatcatcgt
    16921 cctcctgtga aacaaaatct tgttgtgtgc gtaggctaaa tccgttttgg tatgcaagcg
    16981 gtagattttc ccgaatttct tgcaaattgc cgtcacggct catcagcact tctagcaata
    17041 aaggtaaatt caaacccgaa caggcaaaaa cattagggtg ggttaattga gaagaggcaa
    17101 gattcatcgg cgtaccacca acaatatcgg tgagaataat caagcctttg tcggtgttta
    17161 actcagcaat ggtttgctcc atctgccgtt taatgtccgc tacattatcc actgctacta
    17221 cgccgatagc tgccacattt tcctgctgcc cgacaatcag ctcggcactc gccaacgccg
    17281 ccctcgcaaa actgccgtgg ctcatcaaca ataacgcaat ttgattagac ataagtcact
    17341 ccttgattta taagtcattc tattaaataa aatgttatac agataaacaa gggaagttat
    17401 tgcacgatat atcgtacaca taagaagaaa tataaagtga caacagatta aagtaggcta
    17461 ctatataata gtaataaaaa acagtaagaa gatattccta ctgtttataa taactcaaga
    17521 atctttcaag acatcctcta acactctgat tccttgttct atctgttgtt cagagatcat
    17581 cagcggtggg agtaacctta acttactttt agcggttaaa aacagcacac ctttgttgat
    17641 acattttgca acaatatcag cggctaaaat gccttcctca aattctacgc cgagcattaa
    17701 acctaagcct gaaacggatt taacttttgg taagctcgct aagcgttgct gtaacaactg
    17761 ccctttttgt tgcacttggg cgagaaattc ggcgtttatt ttatcgatta ctacatttgc
    17821 agcagcacag gaaacaggat tgccaccaaa ggttgagccg tggtcgcttt tgcctaatgt
    17881 catttccact ttttcgccta acaggaaagc accaatcggc aagccgccgc ccaagccttt
    17941 agcgagcgtg actaaaccag gagttaaatc aaattgttga taagcaaaaa gtgtgcccgt
    18001 tcgcccaatg ccggtttgca cctcatcaat cataaataaa atatcttttt cttggcacag
    18061 gctttgcacg gcgtgcagat aatccgcctc taatgggcaa acaccgccct cgccctgcac
    18121 cacctccagc aaaatcccgc aaacgttctc agtttccaag cgagctttca gtgcctcaat
    18181 gtcatttaca ggtaaatatt caaagccctc tgtaaagggg aaaaaatgct tgtggaatac
    18241 ctcttgccca gtggctgcga gggttgaaat ggttcgtccg tgaaaagagt tcacgagggt
    18301 taaaaccgtt gaacgaccgg aaccgtattt gtcgaaacta tatttgcggg cggttttaat
    18361 tgctccttca tttgcctctg cccctgaatt gcaaaaaaat gcccgtttta gaccgcttgc
    18421 agccactaat ttttgggcta attggcttgc cggttcggta aaaaacaggt ttgaggtgtg
    18481 agctaatttg ccggcttgtg tggtaacagc ttgtagccaa tcctcatccg cccagcctaa
    18541 actatttacc ccaatgccac tggtaaaatc aagatactta ttgccctcaa aatcccacac
    18601 ttcacagcct tttccgtgcg acagtgcaag gtcaaatctg ccataggttt gggcgatata
    18661 atccttatct aattgcttaa tcgtattact gcttgtcatt tcactttcct gttatttttt
    18721 ataaaaccgt gtgccgtttt tgccaccgaa catttctacc aaaatggcgt ggtcgattcg
    18781 cccgtcaata atatttgcct cagagccacc agctctgatg aaatcggtac agcacgcaat
    18841 tttggggatc atcccaccgg caataatgcc ttgctcaatc agggactgca cttcgcttac
    18901 ttccacttcc ggaattaagg tgctttcatc aaagcgatct cgcaataaac ctgcaatatc
    18961 ggtcatcgag accaattttt ccgcttgtaa cgcaatcgca atctcgctgg cagcggtgtc
    19021 tgcattaatg ttataagcct gcccgttttc atcaacgccc accgtggaaa caaccggaat
    19081 caagcctgca ttaagagcaa tttcaatcac agttggatcg actttcacaa tatcgcccac
    19141 aaagccgtaa tccacctctg cctctaattt tttgcattgc aacatcgccc catcaatacc
    19201 acacagccca atgcctttcc cttttaataa ggcgacaagg cttttattag ctttgcctgc
    19261 caacatttgc aaaaccacgt taatcgtatc gctatcggta acacgcaacc cgttgataaa
    19321 ttgcggttct ttacccaata atttcacgcc ttgtgaaatc tccggcccgc caccgtgtac
    19381 cagtaccact tttacgccta aacgatgaag taacagcaga tctgccatca ctgatttttt
    19441 cagttcctca ttaatcattg cgttaccgcc atatttcacc acaatgattt taccctgcca
    19501 tttcaccaag caagcggtgg atttttctaa taaatttgca aattgattga tttcttgttg
    19561 ttgcatattt ctttctcttg tagggacagg tcctgtgcct gtccgataat catcgcccat
    19621 attcggacgg gcacaggacc cgtccctaca tttatattta aagctctaac ccctcaacct
    19681 catctcttcc caacataata ttcatacact gcactgcggc acctgatgcc cctttgccta
    19741 aattgtcaaa acgggcagca agtaaaagtt gggtgctgtt gccgtaaata aaaatctcta
    19801 agctgccttt acctgcataa gcattcgcgg caagcattcc gtcttcgggt agagattgga
    19861 aagggtgaac gtgaattagt cggctggttt ggtagtaatc ggcgagcaaa tttgcaattt
    19921 cttcgccgga tttgaccgct tgtaaggcga agatcggcaa tggcacagtg actaacattc
    19981 cgctgtaata atcggcaacc acaggggtga aaatcggtgg gttttgcatt tgggtaactg
    20041 cctgcatttc aggcaaatgt ttgtgttgga gagttaaacc gtaaacacga ggggcattga
    20101 aagcatttgg gctgttttca ttttcatagt tagcaatcat cgctttgcca ccgcctgaat
    20161 agccggttaa ggaatgacaa cttaaagggt aatcggcggg cagaatccct ttttccacca
    20221 gtggacgaac caacgcaata tagcccgtgg catggcaacc cggcaccgca acacgcttgg
    20281 cattttggat tttttcacgt tgtccgctca attccggaaa accataaacc caattaggat
    20341 tgacccgatg agcggttgaa gtatcgcaaa ttcgggcatt ttccggtgca tttgctacta
    20401 attcttttga ggcttcatcc ggcaaacaga ggaaagttaa atccgcctct gcgacttttg
    20461 ccacacgggc gttcaaatct ttccgctgct cttcgggcaa ggttaataat tcaatatcgt
    20521 ccgcttggct taaacgttcg tgaatgcgta gcccggttgt tcctactgcc ccatcaacaa
    20581 aaatcttgta tttgctcatt gaaaatcctt ctttgacttt ttctgctcgg tagtctaagg
    20641 ctatttaagg ctaaactcaa gtttattttt gaataattat tcaaattaat tgattttata
    20701 ttcaaaaatc cctaacaaaa tgttagggat taaaaagagt attaaaatct attcatatca
    20761 agcttcaggg ctttttgatt cgctttttgg taagcagggt gattttccac ctgttctaaa
    20821 tagcgtgcaa tattcgggta atttgccatt tcaacaaaat tacgccccca ttgtagtggg
    20881 aaacagagag aaaaatccgc accggttagt tcatcaccca caatccattt tttatctgaa
    20941 agtgtttgct caatatagtt aaaatacagc ggagtctgcg cttttgcgta gtcgccaaaa
    21001 gctccgagat cagagcgatt agccaacatt atcacaaggt ttgccgagaa taatgaagca
    21061 gaaatcgaca accagtagtg atacattgaa taggcttcgc taccacgagt cggctcaaat
    21121 ttcccttcgg cgtaacgggc aatcagatgc tggataatcg cattgccttc ggcaagcacc
    21181 aaatcgccat cttgcaacac aggggctttg ccaagtgggt gcaaggcttt tacctccata
    21241 ggtgcaaaac cgctctcttc acgatggtag atttttagct cgtaatccaa gcctaaggct
    21301 tccaacaacc aaacgatgcg gtgagagcag gaactgttca ggtaatgtaa cgtaatcata
    21361 atttttctcc ttcagataat tgaaaaaatg cggtcactta attgcaaatt tttacaaaaa
    21421 aatgaccgct tgtctgtaat gattaaatat ccattggttg ccagttttgc cctttaagcg
    21481 gcggtaacca agcaccacct tgcacgcagt agccccatag tctatcggga atgccgtagt
    21541 ggtcggcttg ttgcgggtcg agttcgctga caatctcact gtctcgatta tctttcaaca
    21601 aggtcgccac gttcgggttg agcattaccg ctttacctaa ggcgataaat tctgcccagc
    21661 ctgttgcata agctttacgg atctgttcac cggttaataa gttacccacg ccgatcagcg
    21721 gtaaacgccc gttgatgtgt tggtgtacca gttgcatacg ggtgaggttg gtatctgcac
    21781 cacggcgggc ttttttgccg aaatcccaga gtgaaatgtg caagtattgc aacggctttt
    21841 cgcacaaagc atcaatcagg gcgtaggtat cggtcatggt taagccgtgt tcgcccggct
    21901 cttccggtga gaaacggtag ccgataataa aatcagggcg ttggtgtttt tcacgcacgc
    21961 tgaccgctgc ctcaaccacc gctaacggga aacgcaagcg tttttcaagg ctaccgcccc
    22021 attcatcagt gcgttgatta aattcagtag agaaaaattg ttgaattaaa tagccgttcg
    22081 ccccgtgaat ttccacacca tcaaaaccgg cttgaatagc taaatcggtg gcattagcga
    22141 aagcttggat catttgcttc acttcgtccg ccgttagggc tttagcaccg ttggcgtgct
    22201 cggacggcgc aactgcattc gccccgtcaa gcaaggcagg aatagcttgt gccccaccgt
    22261 ggaaaatttg caaaatcgct ttggtgcctt gatttttcaa gatattggca gtttctgtca
    22321 ggcttgccaa atagcgttca tcaagagctt ccggctgacc gtaaaacgct ttgccgttac
    22381 gcattacccc ggttgctgcg gtgataaata agcccatatc tgctgcacga ttatgcaaga
    22441 aatggcgttc ttgctcgcta attgtgccgt catcgttgga ggcggaatgg gtcattgggg
    22501 caactacaag gcggttttta atttctacat cgttattcag agtataaggg gtaaataaat
    22561 ttatcatcgt attctcctaa aaaatacggt tgatttcgca aatttttgcg aaaaatcaac
    22621 cgcttgttta ttaccaatgt aaggtcgcac tctcttccac cggcacacgc cctaaatggt
    22681 gtttgttctg catagcgtcc caatctgggt gtccgaaaga aatggcgtgg aggagcttgt
    22741 aatgttcagg aatgcctaac tcttcacgca catctttagc aaacatcgca agggcaagct
    22801 gcggaatgcc gccaaaacca cgagcggtga gtgagagcaa gaaagtttgc gaatacatac
    22861 caacatcgct ggcaatattg acatcgtgtc cgtcaatgct tggcataaat aggaatgcca
    22921 tatgtggcgc accataaccg acaatattgt catcatacac cacttggcga ccggctttat
    22981 cttcacgggg aatgccatag ctggtggtaa aaacgtggct gtattggtta cgccaacgtg
    23041 gttcgtagtc accggtaaat ttcgtttgat tgaactcaaa atccgcatca ttaatgcccg
    23101 cttcaaaagt ctttttaaaa cgagctttta agcgtgctag cgtttcacca gaggcaacgt
    23161 gtactaacca cggctgtgta ttacaagccg aaggggcatt ttgtgcatcg gcaagaattt
    23221 gtttgatttc agtttccgtc atcggaagcg gtaaaaactt gcggattgat tgtcgtgttt
    23281 ttaccgtggt ttcaaaatct aacattttca tttcccctat ttatttttta taagtcaaat
    23341 tcactcacga ctttaacgtg taaacgcaca tctacattac cacgggttgc atttgaatat
    23401 gggcaaactt cgtgtgcctt gctaattaaa gttttcgctt cttccgcact aatgccttcc
    23461 acagtaatgg taatatctaa atctaagccg aaagcaccat cagctttttg accaatccca
    23521 actgcaacag tggtactgga tttggttggc ttaatgccta aggttggggc aacgtgattc
    23581 attgcactgt caaagcaggc agcatagccc attgcgaata attgttccgg attagtgcct
    23641 actttgccac tttcattttg gaaaccgact aaatcaaaac cgatagaacc gtcatccact
    23701 tgggttctgc cgtcacgacc accggttgca gttgctgatg tgtgataaaa aattttcata
    23761 ttttgctcct attaaatgct ttatttgatt ggggatatta tccgcttttt gctatttgtc
    23821 gtcttgcctg attttctata aaatatgcct aattttctaa aagtgagatt attatgcaaa
    23881 ctcatcgagc tattattgaa tctatttttg ccctcgctcc gaaaaatgaa ctttgggcta
    23941 ctcccatttc agggctaacc gttcacgctg ccgatcgttc tattagattt aacggttata
    24001 tccaagaccc aagtttatgt gtagtattat taggcgagcg gcaaatctgt accaatggac
    24061 attgccaagt cttcggtaat caggaaatga tgttttgccc tgtgaatttg ccgattgtta
    24121 cccaaacctt atcagttgca gaaaatcagc cttatcttgc gatttcacta cgcttggatt
    24181 ttgatcttat tgccaaaatt atcacgcagc ttcccccaca gaaaataagt gaaaacatta
    24241 atcaacgcac taaatgggag ctggagaatg aaattaaaga ctgtttgtta cgcttactaa
    24301 atttgcttaa ttctccaaat catattcatt ttcttgcacc attgatatta aacgaactct
    24361 atttctattt attgcaatct gagcaaggtg gctatatacg ctctctactc tctcaagatg
    24421 gcaatattgc taaaattgcc aatgcaacaa aaatcttaga aagaaaattt gccgatacac
    24481 taaatatgtc agcactggct gaagaggtag gaatgagcgt ggcggggttt tacgctcatt
    24541 ttaaaagcgt aacttcaatg acaccactgc aatatcagaa atcgttaaga ctaaatgcgg
    24601 caagggaaaa actacaagca ggagtaagcg tgagtgaaac cgcctatcaa gtcggctatg
    24661 aaagcccaag ccagtttagt cgagaatata aaaggcagtt tggcgaaagc ccgaaaagaa
    24721 gataaaggaa gaatcaaaaa taacaagcgg tcaaattctg caaatttttt gcaaaatttc
    24781 accgcttgtc aggttagaaa ttactgctta tcgccaatta atactgactc taacgcaatt
    24841 tcaatcatac cattaaaggt taattgacgt tcttcagagg tagtttgctc accggtgcgg
    24901 atatggtctg aaacggtgca aattgctaat gcttttgctc catactctgc cgctaccgca
    24961 taaatgcctg ctgcttccat ttctacgcct aaaatgccgt atttttccat tacatcaaac
    25021 atttcgacat caggtgagta gaataaatcg gctgagaata aattaccgaa acgagcttca
    25081 atgcctttcg ctttagctgc ttggtgagca gctaatgtca tatcaaaatc agcaatcgcc
    25141 gcaaaatcgt tatcacggaa gcgaatacgg tttactttag aatcggtaca agccccaaca
    25201 ccgatgacaa tatcacgcac tttgacatca ttacgtaccg caccgcacga gccgacacgg
    25261 ataattttct tcacgccata ttcagtaatt aattctttag cgtaaatcga gcaagatgga
    25321 atccccatac cgtgccccat tactgaaatg cggcgacctt tataagtgcc ggtgtagcct
    25381 aacatattgc gaacattggt cacttctttt gcatcttcta aaaaggtttc agcgatatat
    25441 ttagcacgaa gcggatcgcc cggcataatt acaacgtcag cgaaagcacc ttcaggtgcg
    25501 ttaatgtgtg gagtagccat tttatgttcc tctttattaa ttaataacct tgaacagctt
    25561 gctgctgttc atcattcaat gttgctaata aattagctaa taaacttgat gcaccgaagc
    25621 gaaaatgtga tgcactcacc cagtcattgc ctaaaataga ttccgcaagt gcaagatatt
    25681 gggcggcttc ttccgaggtt tttaccccac ctgcagcttt aaagccaaca cggtctgcca
    25741 cacctaaatc acgaatggtt tcaagcataa tgcgggcaga ttcaagggta gcattcactg
    25801 ccaccttgcc ggttgaggtt ttaataaaat cagcccctgc ttgaatcgaa atttcgcttg
    25861 ctttgcgaat aagctctgct gattttaact tgccggtttc aataatcact ttaagcaaca
    25921 cattattggc agcacaaact gctttgcatt gttttaccaa gtcgaaacct atttgctcat
    25981 tacctgcaat taatgcttta taagggaata ccacatctac ctcatctgcc ccataagcaa
    26041 ctgcggcttt ggtctcagct accgcaattt caatatcatc attaccgtga gggaaattcg
    26101 tgactgtcgc aatttttact tgctcgatac cttgctgttt caatgctttg cgggcaatcg
    26161 ggataaaacg tggataaatg caaattgctg ccggtgtgcc aaacttggtg ttcgcttgtt
    26221 ggcataatgc gatgactttc tcatcagtat cattgtcatt taatgtggtg agatccatca
    26281 acgaaagagc aatctcagcg gattttttta aattcatagt gtctcctttt attaaatgcg
    26341 tggtggttgg taaaatttta taaaaatctg accgcttgta aatagacaaa ccaaaagcaa
    26401 ccgtttacgt cagataaaaa aagataaaac cactatttct agtggtttta tacaaactgg
    26461 attagagaac tgctccaccc aagccgataa acaagccggc aatggttgca ctcattaagt
    26521 tggcaagtga gccagcaatg acggctttaa tcccaagtcg ggcaatatca ccacgacggc
    26581 ttggagccat tgccccgata ccaccgatta aaatcgcaat cgagctgaag ttcgcaaagc
    26641 cacaaagtgc aaaggtaata atcgcaacag ttttatccgt taaatgtacg gttgactcag
    26701 gagctaagta tttagcaaac tctaaataac ccacgaattc atttaccgct aattttaagc
    26761 cgatcatctg ccccgcaatt tctgcttctt cccaaggcac accgattacc cacgcaagcg
    26821 gtttaaatac ccagccgaaa atttgaccta agtttaaggt atcataaccg aaccagccgc
    26881 caataccgcc taaaatacca ttcactaatg caattaacgc cacgaatgca attaacattg
    26941 cccctacgtt taatgccagt tgcatacctg aagaagcacc tgaagctgct gcatcaagaa
    27001 tatttgccgg tttttctaaa tctaccgctt ctagttcatc acggaatttt tcggtttgtg
    27061 gtaccataat tttggcgaat aataaccctg ccggagccgc cataaaggag gctgcgatta
    27121 agtaagttaa tggtacgccc attcctgcgt aaccagccat aacagaacct gcaatagaag
    27181 ctaagccccc tgccataatc gcaaagagtt cagattctgt cattttgctg atataaggtt
    27241 ttactaccaa tggagcttca gtttgaccaa cgaaaatatt cgctgccgca gacatagatt
    27301 ccgctttaga tgtacctaat gctttttgta ataaaccacc taaaatacgg attacccatt
    27361 gcatcacacc aatgtagtaa agcaaagaaa tgagtgcaga gaagaaaaca atcaccggaa
    27421 gtacacggaa agcgaacaca aagccgccgc caaatacttc aaacattttg tcgctgacta
    27481 agccaccgaa taaaaatgaa atcccttcat taccgtaaga aatcactttt gacacaccgg
    27541 ttgccatcgc attcaaggca tcacgccctg ccggcacata aagcaccaat gcaccaatag
    27601 ctacctgcaa tgctaatgcg cctaatacgg tacgcacgtt tattgcacgt ttattgctcg
    27661 aaaataaaaa tgcaatgaat agtaaaacaa taatgcctaa aaagctgtta agagtaccca
    27721 tagaacctct aattataaat tgattaaaaa aggtgacgat actataccta tttttaggca
    27781 aaaaatcttg tgaaattatc atcattcaag acaaaatttg acaaaaacta aattatggta
    27841 ttttattatt catcacaagc agtcaaattt atcgaaaaat ttacaattat agagaggtaa
    27901 tatgcaaaat attcgtgaaa tttacttagc cggtggctgt ttctggggcg tggaatcttt
    27961 tatgcaacgg attaaaggtg tcattaaggc acaatcaggc tatgccaacg gtaataccga
    28021 taacccaagc tatcaagagg tgtgtgccgg tagcggacac gctgaagtgg taaaagtgac
    28081 ttatgatgcc aatcaaatta gcctggcaaa attactagat tacttcttta aagtaataga
    28141 cccggtgagc attaacaaac aaggtggcga catcggtatt caataccgca ccggtattta
    28201 ttataccgac ccacaagacg aaccggtggt aaccgccgca cttgcacaat tacaaaccca
    28261 atttgatcag ccgctggcag tggaaaacca aatgttagag caatatttcc ctgccgaaga
    28321 gtaccaccaa gaatatttag taaaaaaccc gaacggctac tgccatattg atattcaact
    28381 aatgaatgag attttgaaga atcaataaca aaaaaccaca gagcaatctg tggttttttt
    28441 agctttattt tctgagcaaa ccgtacactt ttttcagtgc tgaaaccggt aataaacgag
    28501 gaattgcacg cattatgaaa atcccaaaac ctgaaatcgg atcgtgaatc tggcagcggt
    28561 tttttaattt atataaacgc cattctgcct ctgcttgggc cattccacgg cgtctgccaa
    28621 ccattccgtt accgactcgg gcatacacta ataaatccgg caagtttgcc acttgtttaa
    28681 attgagcaat gagtttgatc cataaatagt aatcttcttg cagatcttga tagccaccga
    28741 cttccaacac tttggatttc tgataagcaa ccgtcatatg gttaaacggg caacgcaagc
    28801 gagtgaatct gacgatttcc tccaccatgg ttggtacatt gcggtaagcg acaagatcgt
    28861 gaatatcttg cccaaattca gcaatttggc cgccaaaaat cacggtttca ggattctgtt
    28921 caataaatgc cacttgtttc tcgaaacgtt ccggcataca aatatcgtct gtatccattc
    28981 tgaaaaccca ttcattctta caataggtta gcccatcatt tagcgtttta cctaaacctc
    29041 gattttgtgg gaatttcacc gcattaatcg gcaaaattgc actatattct tctacaatcg
    29101 cctctaattc aggcgtaact gtaccatcaa aacaaacaac gatttcatca gcctgcacag
    29161 tttgtacttt caagctttca aaacattctc ttaaatattg cggttgctct ttaatgtata
    29221 acgacattaa aacggaaaat ttcattacat aaaccttttc aaattaatcg ccaacttcgg
    29281 cgaaataaaa ataaccaacg caataatcag ttgtttgata tttttagtct gtttaaacat
    29341 ctcaaaataa taaccggctg ccttgcggga taaattcatt acgtgtggat aacttaaaat
    29401 ataaagcgaa ttgagcttaa aattattcgc ctgctcaggt gttttgatat acgtttgttg
    29461 aatatactca atcgcctttt ctgtattagt ggtatcggtt gaaacactgg agcgtttggt
    29521 atggaaagta caataggtca aggcttcgtc cacatacaat ggctcaaaac tcggttcttc
    29581 caataatttc agtaagaaat cataatcttc aagtgatcgc aagcggtcag ataatccacc
    29641 aattttacaa aagagtgatt ttttcaagcc aatcatcggc attccgccaa ctttattcgc
    29701 ttttagaatg ttttctttag ttagctcact tttatgttta aacggcttgg tgacataagt
    29761 aaaaccctca tttaccattt cacattttgc cgggtggtac acaaaattgg cagttggatt
    29821 gttcgcaatc tctgccgcta aacgttcgca tttttcaggc ataaagcggt catcatcatc
    29881 taaaaacagt agccattctt gtgtggcatt agttgcccct acatttcggc tttgtgccgc
    29941 cccaacattt gggttatttc gcaccacttt cacatcaaat ggatagctat aaatatgaga
    30001 taaactatct ctagtaagta attctcttac tttttcttta gtagaaatat cgttaaattc
    30061 atttaaaatt cctaaagttt cactgaccgg tatttttgag cagtcatcaa caatgatcac
    30121 ttcaaaatta tatgccgttt gttgctttaa actctcaagc aacgcattga tttcttgatt
    30181 acggttataa gaaggcacaa taatactaaa catattcagc ctctttgttt ttgcaaaaaa
    30241 atgttacatt tcgaccgctt gttccaaaca gcgacaagaa cattagaaac agcatcatca
    30301 gccccgggaa agcaaaggta tctgctttaa tatttaaaat agctaaaatg ccgatggtgg
    30361 ataaggtaaa aatccaactg ccttcaaacc aattcaccgc cactttgcgt acaaaatagt
    30421 aagtgaaggc aaaacacgcc aacatataac ttactccacc atataatact tggcgaatat
    30481 agccggaatc cgatccacca taatagccac cagtttcggt gtaatataaa ccatcaccgg
    30541 aaataaactg atttaaagtc ggtaaaaaca gatggttttt cactaaatta tcggttgaag
    30601 agctgacttt tccggtgccg gacaataaat taattaccgg ttctaaggcg tgttcaacat
    30661 aacggttttc cggaaaaaca atcgccaaaa atagaattaa cgcagtgaag gcaatcagtg
    30721 gcaatatata tttccattta aaatagagtg caatacttat tactgacatt aataaaaagg
    30781 ttctaccgga aattaaacca acacataata ataaaaataa aaaccactca ttcaccacat
    30841 ctgttttttt attatatgcc aataaaaaat gtaatagcat taaataaaaa atgcttaatt
    30901 ggaaaaaggc ggaagaagtg atgttatata aacggtattc ttgttccgaa ccaaaaaaac
    30961 gtggcatcat tgcattgctg gataataaaa aatccaccat aaacggcaaa cctaataagg
    31021 ctgtcacacc acaaatccat tgaatagcaa tgccgatttt taaatcacga acgaataaag
    31081 ttttaccttg ttggttatga taaaaaacat tataaatcgc aatcccgaac acaaacaaaa
    31141 tcagcatttt gacatacatt gccagcacac caatttcttt tgtactgtta atcaataatg
    31201 gaataatgct aaagccgatt aatgcaataa ttgcaatcag gctatcttta ggaataataa
    31261 tgccattcga tttgcccttg attatttgga aaaccaaata gccacaagca acaagcccga
    31321 ctacaaacga cattcttaaa aaatggaaaa accacggatc gtaaagataa aataagttaa
    31381 tgagtgctga catttcagac ctattatgaa tttcgctgag taaaattacg tttaattgcc
    31441 agtaattttt taagtggata ttttacggct tttttaaata aattattatt taatgaacca
    31501 cgtaaatctt ccaaattact aattagaaca ggattttcta tcgccattaa cgggcggaca
    31561 attaacacat caaatttgaa attcttttca aataaaataa aatcatcggc aagccagtat
    31621 ggattttttc cggcacattg tgctaaaaac tgacaagcgg tcgatttttt aattaaatat
    31681 gcaaccgtac cagcaaaata attcaggtaa gaataagcat atttatattg gctattcgga
    31741 atattttttt gcaaaaagcc aaaggttgat gggtaattaa gttctaattc gctatcgtta
    31801 aaagttggaa ttttcgactg cccaactaaa ataatatcga cttttaaatt ttgtgctaat
    31861 aaagcatcaa gatgtttttg gaaatcatcg gcaaataagg catcatcttc acagactagg
    31921 cagtaatcat cttccgcaat agttctatct tcggcaattt tttggtaaac ggctaaatgg
    31981 cttaaagtac aacctacttc gcccttagtg gttttacgcc catatcgctg ttcaaacaga
    32041 gccaaatcaa aatggttatt taatgcctcc caatcactac tcattgtatt aatggcactg
    32101 aacacctcaa aatcttccgt attaggctgg ctaaaaaata gctctcgtct ttttacatct
    32161 ttttctaatg aaattaaata tttcttcatt ttattctcta atatcaacaa tctgaacgcc
    32221 taatttttca agcaattcat agctctcaat ataagcagga ttatctacat tcactcttaa
    32281 tgctaccact tcaatatggt tacttttatt taaaatattg atcaccgcac tactaaaata
    32341 agtatagact ctacattttt tatggctaaa ttcttggaaa atataatctt caaaaattag
    32401 attagtatta atgtattcta cattttctaa ttcatatttt tctcttggat gcggtaaata
    32461 tagatcaatg ccaaattgtt ggataacttt ttccgccaat gctatatttt tttcatcatc
    32521 tcgttcaaaa ataggctgtc ctaataaaat atgggtaata tcatcgttag atggtgctgt
    32581 acccactgat tcaacctgat taatgagatc aatataaacc gtattttcaa taatattcgg
    32641 aaaatttttg taaattgtat aatgagtttt agagcgttgt ttcagctttt ctaggctata
    32701 tttattacct aaaaaaaagt taatcaattt tcgatttaac ccaatatttt cagcttgata
    32761 gaaaatactg ttttgtgcaa tattagctgt gccatcatca aaggtattga gttcttgaaa
    32821 tgaaatggca cttaacagaa attgaatttc taactcattg atatttgcta aaaagacttt
    32881 attaaacttc ttaccgccaa aagtacgttt taaataaata atttctttta ataattgaat
    32941 acgctcgcta tgttgttgca tcgcaaaaaa ttgttcgcac ttttgggcta atctagcttg
    33001 gtaaaaatca aatttcgcgt tttttaccgt agaaagcatc accccataga atcgctcatt
    33061 agggtgcatt tcaatgattt tttcagcaat caacacttgc aagggcgtgc aacaaataat
    33121 taaattcata cggtttttcc tacttttgcc gtcattatat aaagaatcgg aataatgctg
    33181 attgcaccaa tgatgctggc aaaaggaata tattcaattt tagtaaaagt taaagcaaat
    33241 agtgcaacta aataaaaaat agtagataaa atcgagcaaa gcgaaattag cttatttttg
    33301 ccataaaaaa acaaataatt gactaaaatg aggtaaggca caaccaacat tgtcgcaatt
    33361 aaaaagagaa taatgtagta ttttgttcct acaaattggc tacccagaat ccataccact
    33421 aattcttccg gaataaacca catcacaata gcaggtaatg gtacaataaa taaggcatac
    33481 aacgcccatt tgtggatatg gctgacatta atttttttct gctttaaggc ttcataaaaa
    33541 taaggcagtg ttgctttatt taaggcttga accacaatgg ctagaatcat cgcaatttga
    33601 gcccccattg catataaacc taaatcctct tcgctaaatt tatggtaaat aaatactctg
    33661 tctaactgtc cttttaagaa aatactggaa tggtgcaaaa tcaacggaat accaaagccg
    33721 aataaataca ataaagcagt tttatggcgg gcaaggctaa atttcttcgt ttttgctctt
    33781 ttagcataca aaaagtaagc gattagaaag actaagccat tactcatcaa aatcgctaaa
    33841 atccgctttt ccactaaatc ggtcttaaaa tactccatca ttgcaacggt aataactgcc
    33901 gaaaaaatcg ccgacattaa ttgaatcgtt gtataagcaa gtgcctgttt ttgacactgc
    33961 cttacactaa gctgtacatt caaaaacgac tggaacatcg ccccaatcgc tacataagca
    34021 ataatttcag actggaaata ccagcaaccc aacaggataa gcaaagttgc acaaagagta
    34081 tataaatacc ctgtactcaa gactaaattt agtgaacgct taccataaaa atagaaatag
    34141 cgagcaaccg caccgtcttg gctaagcccg ataaaaatta caaataaagc ggcaaaggtt
    34201 tggtagtagg aaagctcccc ataacctgcc acgccgagtt tgcgtgaaaa gtagggtaag
    34261 agcaagaaag gaattgactt tgacgccaat tccccaatta aataaatcac actgtcttta
    34321 actgctttca tagttgcaaa ttattctgat tttttgaccg cttgcgagaa agcaacatca
    34381 taaaatcctt tcggttgagt atggtggctg tctgctaaga atattttgat tgcattgtaa
    34441 gccttttcca tcgcgttgtc ttgaaaatat ggatttttca tactcggtaa acggtgttgg
    34501 aaatcggctg aaatcaattt ttcgatacca tctatgatag cctgttcatt actctctaca
    34561 tcaattacgc tctcaccccg tactcgccct tgctggcgat taccgatatt tagcgtacca
    34621 acaccacaac tcggtgcttc aatcaagcct gatgagctat taccaatcaa cccattcgaa
    34681 tatttaatca acgctaaata ctcttccggt ttgactgagg tgaaaaactt aaattgattt
    34741 tcatcggtgt aagccttaaa acgtgcataa atttgctctg aattggtgtc tgaatttgag
    34801 ccgataaaga cgaattggta tttgtcttta aactgctcaa aagcattgag taacgcctca
    34861 atttgctcca acacggaaac atcggttaag gtttccgggt ggaagaccac taaaaaataa
    34921 ggcgatgccg gaatccctaa tctctcgact aattctgctt gggtcggcaa cactaaatcc
    34981 aaactattct ccgcccctag agaaccagta ttcaccacat tttcgggcag ctcgcccatt
    35041 tgaatcacac gctgtctata ttcttcagtt gaagttaaat gcaatttcga cattttggta
    35101 atacaatggc gaataaattc atcgtaatta cctaatgttt gctcaccacc gtgcagatga
    35161 atcagcggaa tattatgcat cgccgcagcc gttgccaccg ccaacatctc gtaacgatcg
    35221 cctagcacaa tcaccgcatc ataacgatgt tgctggaaat gttcgccaaa tcgatctaaa
    35281 cactctgcca ttgaatgaat aatggtctga ttattttcac tgtttaaggt aagcggaata
    35341 cgagctgcaa tctcaaagcc atcttgccca atgaccgtca ccgtattgcc atattgcgga
    35401 tccaaatgca tcgcagtaac aaccagagag aaattcacag tcggatcttg tgctaatttt
    35461 tgtaataaac gctttacaat cccgtattcg gcacgagatc cggtaatata ggcaatattt
    35521 ttcatttagt tgagctcttt ttccaaaata gcggttaatt tttcataacc ttttgcattt
    35581 aaatgtaatc cgtcatcgct atagcttaaa tccaatttgc catatttatc gctaaatgcc
    35641 tgatctaagc taatccattt caccgctaaa ttatctttaa gatattgatt taacaagcga
    35701 atatcgttgt tattacgatc gactctaaat gcagttaggg taatttccaa gaaatagaga
    35761 ttaatatctc cacgaattgc tgttaattgt gcaattaaat gattaatatc tgctagaatt
    35821 ttttcatcac tccagccagc atatacaata tcattagtac cgaacattaa aataatgtct
    35881 ttaggtaaag cagaaattaa accttcatcc aaaatcagat cactgtattg ttttgttgaa
    35941 atacctgcaa ttcctaaatt attaactgcc ttaccgttta aatgttcaat atcccaatat
    36001 tcaatgatcg aatgcccgat taaggctatc tctttcactt ctttaaagtt ctcttttttc
    36061 attaaaatcg aacgtttaat ggtttcaagt aagatttttt ctttattgcg ttgttgcaga
    36121 ataaagtaga agtactcgaa atcttggcga tcatcaatat caatagaaac gtctttatcc
    36181 ataaaatagg ctagacattt cgcaccatag aagtgtcttt gtttaagata ggcttctggc
    36241 ttagctgtaa aaatagctcc attcggtgaa tattcaggat tatgcaattg gcgtgcatag
    36301 ttagaataat ccaactgaaa atgctttagg ctctcgtctt cctcaatcat acgagtcaat
    36361 gtcgtaggtt tatgggcatc agaaacagat actaagaaat caaattgttc gaaatttttc
    36421 tcaaattttt cacacgattc cttaatatgc tctcctgatc tcaacggcga agttggttga
    36481 agtagcataa aatagtcaaa ctctactgat ctatattgat ttaacacatc ttcaatcacg
    36541 acaaaagtac tcgccttatc actggctaaa tgctcggcac gtttaataaa ttcaattgga
    36601 taatgtgaga gcaaatcaat atattcttgg gaatcagtac taacgataat tttctcaaat
    36661 tgccccgact caatcgcagc ttcaatagaa taggccatta acggcttacc gttggctaat
    36721 aatacatttt tattcggcaa gccttttgaa ccggctcggg ctgtaataat tgcaattttt
    36781 ttcataaatt tgaccgcttg ttaaacacaa aatgatggag ctttcatccc atcactatat
    36841 tatattacat ctgattttca aaacgagaat cttgaattaa ttgatcttct tcaaaatcct
    36901 gttgtgcttc tttacctagc acatcatacc aaaacatcgg gctaatgcca ttaccggggc
    36961 gtttagtcgt aatattttcg gttgtaaaaa tttcgccttt tttaattgga cgtgccgcaa
    37021 taattgattt tcttgccaca attttatttt ttgcttccga attagtgact aatttctcgg
    37081 aagaacccag cgatttttcc accgcacgaa tgccttcaca cagtaatttc agctcttcag
    37141 gtgtaaccga agctttatga tccggacctt caaagttttt atctaaggta aaatgttttt
    37201 caatgaaggt aatgccgtaa ggtactgccg ccaaccctgc aaaataaccg gcagagtggt
    37261 cagaaaaacc taaactatag tcgccaaact cctgttttag ctgatggaac gcatttaaat
    37321 tcacatcttc ataaggtgtc gggtactcgg tattacaatg taaaatcgta atatcttttt
    37381 tcgctacacc gttatcttcc aatactttta atgaggcttt tgtttcttca atggttgcca
    37441 taccggtaga aataacgatc gtcttgcctt caatcggaag tcgtgcaatt ttttctaaat
    37501 acggcaaatt ggttaattca ccagacggaa ttttccacac tttttgttgt tggcttgcca
    37561 agaaatcaat cgactcgaaa tcgaatggcg tagagaatac ctctaacccc aatgaacgag
    37621 cgtaagcctc taatttcaca aactcctcat aaggtaactc taatttgcgg gtcatttcta
    37681 attgggaatc agcggtgccg gtcgttactt tttgatactc ggctttaggg gcatatttag
    37741 agatcaactt atccgcttta aaggtttgga atttcaccgc atcgacacca cactctttcg
    37801 ctacatcaac cattttcttt gctaacgcag gatcgccgtt atggttacaa ccgatttctg
    37861 ccacaataaa tacatttacc attattttat atccttaata tattttgctg gaacgcctgc
    37921 tacaactgtg cgaggctcca catttctaat taccactgca ccggagccaa ccaatgcact
    37981 ttcacccact cttagctgtc cgttcacaac agaggaacta ccaataaaag catgatcttc
    38041 gacaattaca tcgccattta gtgtcgtatt ggttgaaata ttgcaatgac tgccgataaa
    38101 acaaccatgc tcaaccagcg atttagtgtt aatgatcaca ttatcgccta cggttacgcc
    38161 actgtttaca attgccattt ttccaacaaa cactccaatg cctagagtgg aatttttgga
    38221 caccaatgcg gttttatcta ccacattaat tattctacac ttatattttt gtagcttaga
    38281 gaatttttct aaacggtaac tattatttcc aatacaaata aaatagtgat aattatctcg
    38341 tttagaaaaa caatcaattg ttgaagctag aataggataa cctaagtgca cactaccttc
    38401 aggcttaaag ttatcgataa atccacaaaa ttcatattgt tcgtgatcaa gtgaatctaa
    38461 cactgatttg gcatagccac cggcaccgat caaaatcaat ttttctcttt tagacttttg
    38521 ttgcattgct atcacccatg atgaatttca attcatatgg agtattctaa tacttttcaa
    38581 gatagaaaaa tagacaagtc ttatataatt taggaaaatg aacatatctt tacttatttt
    38641 tactacgata ttctttctat gttcgagttc attaaactga tcaccgcaat gatcctcccg
    38701 ccatttaata ttattttgct gtggttgtta gcattaatcc tcttttcttt aggctataaa
    38761 tacgctgctt acttctttgc cttgttaggt attctgattt tatatatctt cagcattccc
    38821 tatacttcac agaaattagg ggattcatta gtcgaagcag agccatttac catagagaaa
    38881 tataaaaatg ctcaagcgat tgtggtgcta ggtggcgggc tacgagacag ccaagagtta
    38941 ttcggcaaac tggcaattac cggtattccg ctggaaagaa tgcgttatgc cgcttatctg
    39001 cataaagaaa ccggcttacc gattttagcg accggaagca gcccaaatgg gacttctgag
    39061 gcaaaggtta tggcagatga atttaaaatg ttcttcaata ttgagaccaa atggctggaa
    39121 aataaggcga aaaccaccaa agaaaatgcg attttcagcc gtgagatttt agaaaaagag
    39181 aatattcata aaatcattct tgttaccaac caatggcata tgaaacgggc tgaaatgctg
    39241 tttagccaac aaggatttga ggtgatgtct gccagcgtcg gttctggcga tacgcccgat
    39301 cattacaaac tcaactttat gcactttatt ccgcaagccg gtgcaatgaa tagcaatatg
    39361 ctggcattaa aagagtggat tggttattgg aaagagaaat aaccgaagat aaatagcagc
    39421 aaggcaggtt ttcacctgcc ttctttttgc aaaaaatcag tcaaaaacaa ccgcttgtct
    39481 aactaatctc ttgtgggtca atgtcgatat tcagcctgat attattcgga atgtttaaac
    39541 tttccctttc ttgatcaaag gcatcgagta gctgttgtaa tgctcctcgg agagggtgtt
    39601 gaatcagcaa taaccagcgg taatgtcctg ctttcttcgc cattggggct gaaaatggtg
    39661 gagggagctg gaagccttgc ccgttttgtt gctggatttt ttggctaaaa taatctgcta
    39721 attgctcaag cagctgtaag atttgttgat tatctttgcc ggtcgctttg aacaaaactt
    39781 gcgaggaaaa tggtggcaaa ctcattaatt gcctcatttt taacgcctca tcagcaaaag
    39841 ctcggtagcc ttgttccagc aaggttttaa gcagtgggtg atcagggtaa tgggtttgta
    39901 gcaccacttc gccttttttc tccgctcgcc ctgctctgcc tgccacctgt atgtaaagct
    39961 gtgctaattt ttcttccgca cgaaaatcag ttgaaaataa cgctgaatcc acattaacaa
    40021 tcgccaccaa cgtcacattc gggaaatgat gcccttttgc caacatttgc gtaccaatca
    40081 aaatttggct tttgccctct cgaatagcgg tcaaatggtt ttccaatgca cctttgcgag
    40141 cggtagaatc tctgtctata cgagtcactt ggtaatgagg gaattgctga tttagcacct
    40201 gctcaagttg ttctgtgccg acaccggtag ttattaaatg ggttgaacca caatgtccgc
    40261 attggcgggg aattaccctt tgggtggcac aatgatggca acgcaacact ttttgctttt
    40321 ggtggtaagt aaaaggctta ttacacgcct cgcactcaca aacccaaccg cattcgtgac
    40381 aaagtaatac cggggcaaag ccacggcgat ttaaaaacag catcacttga ttgccttgct
    40441 ctaactgggt tttcatcatt gaaagcaagc gctccgacaa accggcagtt acccgttgcg
    40501 tttttaaatc aataatctgc tgattggcaa gttgtgcatt gccagcccga gcggttagtg
    40561 tcagttcgat aaatttcccg ttttgcacat tgtgcaaact ttcaagactt ggcgttgccg
    40621 agcccagcac aatcggaata tttgcatttt tcgccctaac caccgccaaa tcccgtgcgt
    40681 ggtaacgcca accgtcttgt tgcttaaacg agctatcgtg ttcttcatct aagataatca
    40741 cgcccaattt ttgaaactgg gtaaataaag cagagcgagt gccgattaca atcgcacttt
    40801 ctccattttt cgcccgcaac cacgcattta accgctcagt ttcgttcata ttcgagtgca
    40861 atacgtcaat ttcaacgtta aagcgagctt taaagcgttg taccgtttgc ggtgttagcc
    40921 cgatttcagg cacaagcagc agcacttgtt cctctcgctt aagcaccgtt tcaattaact
    40981 gcaaatacac ttcggttttg cccgagcctg ttacgccatt gagtaaaaag ctcgcaaaag
    41041 tggtttgtgc atttaaacgg ctgaccacta aggtttgttg tttatttaag atcagccgat
    41101 tgttagtatt tactaacgga aattcaccta gtgagtcttg ccacgcttgt gccataaacg
    41161 gcacatcaat tttctcgata tacccttttt cgagtaagcc tttccaagca gatgggctac
    41221 aagcggtcgg tttttcaaaa tattttgcac attcggcaag ctcggttaac agttccacct
    41281 gctttttggc ttttttattt tcaccgataa tcagagattt ccgcccttgc tccgtcacca
    41341 caaa
//
LOCUS       DANKBV010000006        37343 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.006, whole genome shotgun sequence.
ACCESSION   DANKBV010000006 DANKBV010000000
VERSION     DANKBV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 37343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37343
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.006"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(1..1248)
                     /locus_tag="PWY43_000232"
                     /pseudo
     CDS             complement(1..1248)
                     /locus_tag="PWY43_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249514.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
     gene            1389..1985
                     /locus_tag="PWY43_000233"
     CDS             1389..1985
                     /locus_tag="PWY43_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HDL2135151.1"
                     /translation="MSLLPMNEITSLNQLDLQKSYSYADYLLWKFKERVEIIKGKIVA
                     MSPAPTRFHQRISMKMTAKFLEVFDNHQCQIYSAPFDVRFPDENGKVKTVVQPDLCVI
                     CDVSKLDERGCVGAPDLVVEILSPGNTKREMKDKYELYQEQGVKEYWIVSPETRTIQI
                     FVLENGKYIGIQPVAEDEPATSVVFPALSFSTEKLFEL"
     gene            2133..2348
                     /gene="rpsU"
                     /locus_tag="PWY43_000234"
     CDS             2133..2348
                     /gene="rpsU"
                     /locus_tag="PWY43_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL2135152.1"
                     /translation="MPVIKVRENESFDVALRRFKRSCEKAGILAEVRAREFYEKPTTI
                     RKREKASLAKRHAKRNFRENARNTRLY"
     gene            2502..4250
                     /gene="dnaG"
                     /locus_tag="PWY43_000235"
     CDS             2502..4250
                     /gene="dnaG"
                     /locus_tag="PWY43_000235"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2135153.1"
                     /translation="MKGTIPRSFIDDLIARTDIVEVINSRVKLKKAGRDYQACCPFHH
                     EKSPSFSVSQSKQFYHCFGCGVHGNVITFLMDYDKLEFPEAIEELAAMHGLEVPRENV
                     IQRDGKPQANFKTKRNLYELMEAVAKFYQQNLSHQIEPQSYLQQRGLSPEIIERFEIG
                     FAPNAFDAALRTFGQSQEDIQKLLDTGVLTKNDSGRIYDKFRNRVMFPIRDKRGRVIA
                     FGGRVLPNDEQGAKYMNSPETVIYHKGSELYGLYQALKVNETPDYLFVVEGYMDVVAL
                     AQFGVDNAVASLGTATTGEQIQQMFRCTEQIVCCYDGDRAGRDAAWRALENALPYLQD
                     GRQLKFIFLPDGEDPDTFVRSQGKEGFEQYVAQNAKPLNDFLFDSLLADVDLSSKEGK
                     SKLAALAVPLINRIPGEMLRVYLRNILGQKLGILDPTQIEALLPTKKAAEKPLHQAVK
                     MKRTPMRLLIALLIQNPYLVKFVPDVSALASLKDPGFTLLTDIVATCQQNAGISMGGL
                     LEHYREDPQYKIVEALANWDHLELPENIERTFIDTLDFLYAKLVELRKEELIAKDRTV
                     GLTDDEKKEYALLMFQ"
     gene            4509..6380
                     /gene="rpoD"
                     /locus_tag="PWY43_000236"
     CDS             4509..6380
                     /gene="rpoD"
                     /locus_tag="PWY43_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL2135154.1"
                     /translation="MENQADQQLEQQSQLELLISQGREQGYLTLSEVHDHLPEELVDA
                     EQIEDIIQMINDMGIQVLEAAPDTDDLLLAENIADEDVVEEATKVLSTVESELGRTTD
                     PVRMYMREMGTVELLTREGEIDIAKRIEEGINEVQCAIAEYPEALSGLLACYAQVETG
                     EMRLSDLVTAFVDPNILEEAPLEDIEAALGEDEESSDVPDVDDENEDEDGDSSSDSSD
                     DSSIDPELAREKFEALRSQHEKVLLVIQKHGRTSKKSKEQIEILSHIFREFRLVSKQF
                     DLLVASMQKLMKQVRTEERQIQRYAVEYAGMKKADFLKAFQGHELSEAWIEKAINAKK
                     NAVPKLANYVDNIRVNIANLQNLEQTSGLTIAQIREIGGRISNGELKARRAKKEMVEA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKLNRISRQCLQEMGREATPEELAERMGMPEDKIRKVL
                     KIAKEPISMETPIGDDDDSHLGDFIQDESLELPLDSATAESLRMATNEVLEGLTPREA
                     KVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDD"
     gene            6474..7460
                     /locus_tag="PWY43_000237"
     CDS             6474..7460
                     /locus_tag="PWY43_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL2135155.1"
                     /translation="MKKSLLALLIGSVFLISACEDGKMTQTVLEAEKQIVQLGTELKN
                     AQADLAKKEAEVAELSGVKAENEKLKSELENAQQNTALNVEIVKIFDKKEVIKHQVDP
                     KQEFAIEESTVSAFVSLPKTNFDWLNQLLINQAYDYNEEQGRKLKNPTEEEFKKHREA
                     VYQGLVESVKTEPAIGYDDHISSAFIGQRNNIATFTMMHYTYMGGAHGMHYTQYINVD
                     LAKKAIIGLNDLISPKNQVQLKDILWENYSSVRVDENGNYNGFADKKEFRISDQFYFT
                     PHGIAFVYPPYELGAYAEGDIEVEADWYIINKLLNSEYQRGEKDGFFEELDN"
     gene            complement(7536..8444)
                     /locus_tag="PWY43_000238"
     CDS             complement(7536..8444)
                     /locus_tag="PWY43_000238"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2135156.1"
                     /translation="MNKNSLFSIWFTTARPKTLPLALASIIVGSALAHWAGKFDVVTT
                     LLAFITTILLQILSNFANDYGDHVKGSDTKERIGPLRAIQHGVISGEQLKKAVIILSL
                     LSFVSGVALSAYAFETVQDLFFFIGLGIVSIIAAITYTVGKKAYGYLGLGDLFVLIFF
                     GFVAVIGVFYLQAHSLPIMILLPAFGCGLLSVAVLNINNLRDINQDRQAGKNTLIVRI
                     GSQNGRKYHVALLVLAVISYLIFAISEFEHWYSFIFLLSVPLLAKHARFVYQHKDPKE
                     LYPILGQMAGLALLTNLLFSLGIVLG"
     gene            8624..9361
                     /gene="tsaA"
                     /locus_tag="PWY43_000239"
     CDS             8624..9361
                     /gene="tsaA"
                     /locus_tag="PWY43_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDL2135157.1"
                     /translation="MNSTSNQLTPIQLNPIGFVESPYDEKFSVPRQPNLVAEGKGILR
                     LIPPYNTPDAVRGLEKFSHIWLIFQFHHIPEREWHATVRPPRLGGNERVGVFASRATH
                     RPNPLGLSKVKLEAVVAGGSEVYLKLGSVDLVNGTPIFDIKPYIAFADSEPQAVSGFA
                     QQKPQARLAVRFSEEAKQAVEFCRNFAKFGIEQPLSFLADVIAQDPRPAYQQGKPSER
                     VYGMNLAGYNIRWQIENDTQAIVLSIE"
     gene            9419..10138
                     /locus_tag="PWY43_000240"
     CDS             9419..10138
                     /locus_tag="PWY43_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253715.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDL2135158.1"
                     /translation="MKLKNFLTILPLAVATAVTSAETHTVEHKDKPASFNFSTEVSRT
                     VEKDVMQASVYSQKSGKSLPELKKFVSENLNRVLAEAKKHPSIEVQAEGVTNHVNYNN
                     KGNIDGWDTSGSIQLKSKDFDAMAAVLENLGKDVAIRYIDFSVSPEKMASLEDEMTLE
                     IISKFQHKADVIQKALGAKTYKLVNINIQTPNDGGRPYYEHRAMYAVSSMAQSKSMDE
                     IPLEAGKTTISATASGSVEFE"
     gene            10333..11019
                     /gene="aqpZ"
                     /locus_tag="PWY43_000241"
     CDS             10333..11019
                     /gene="aqpZ"
                     /locus_tag="PWY43_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDL2135159.1"
                     /translation="MKKYFAEFFGTFWLVFGGCGSAVLAAGIPELGIGYAGVSLAFGL
                     TVLTMAYAVGHVSGGHFKPAVSIGLLVGGRFNAKDLVPYIIAQVIGAIAAGAVLYTIA
                     SGVPTFDATAGFAGNGYGEHSPHGYSLTAALLIEVVLTAFFLIIIMGATDKRAPAGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTGVALFQGSWAIEQLWLFWVAPIVGAIIGA
                     VVYRFIAEEK"
     gene            11091..11231
                     /locus_tag="PWY43_000242"
     CDS             11091..11231
                     /locus_tag="PWY43_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135160.1"
                     /translation="MKKLIKVCGLISATIFLSGCLVTSVVGGVVGTAVDVVDTVTPDI
                     ID"
     gene            11334..11927
                     /gene="slyD"
                     /locus_tag="PWY43_000243"
     CDS             11334..11927
                     /gene="slyD"
                     /locus_tag="PWY43_000243"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2135161.1"
                     /translation="MNITKDLVPSIAYQVRTQEGVLVDEAPVNQPLEYLHGHNNLVEG
                     LEKALEGKAVGDVFEVRVKPEEGYGEYNDNMVQRVPKDVFMGVDELEVGMRFIADTDL
                     GPLPVVITEVDGDEVVVDGNHMLAGQELLFSVEVVGIREATAEEIAHGHVHGGHEHGC
                     GCGGHDHHHDHDGCCGGHDHDHHHHHGHGGCGCGGHH"
     gene            12188..13720
                     /gene="ilvA"
                     /locus_tag="PWY43_000244"
     CDS             12188..13720
                     /gene="ilvA"
                     /locus_tag="PWY43_000244"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDL2135162.1"
                     /translation="MMNETQHSQSAIDYLRSILSSNIYDLAQVTPLQKMEKLSERLGN
                     SIFIKREDRQPVHSFKLRGAFAMISNLSKAQKEAGVIAASAGNHAQGVALSAKHLGLR
                     ALIVMPQNTPAIKVDAVRGYGGEVLLYGANFDEAKAKAIELSTELGMTFIHPFDSPAV
                     IAGQGTIGLELVQQKPDLDYVFIPVGGGGLIAGIAVLIKQVMPNIKVIGVESKDSACL
                     YHALKQGQPTDLERVGLFADGIAVKRIGDETFRLCQQYVDDVVLVDNDEICASMKLIF
                     ENVRAISEPSGATSLAGLKKYVKEHQLQDKNLACILSGANVNFHTLRFVSERCEIGEK
                     REALLAVTIAEQKGSFLKFCEILGNRAVTEFNYRHSDDKQACIFVGVRISGSAEKVEI
                     IKDLQQNGYDVADLSDDDIAKTHIRYMVGGRASSIQYEQLYSFEFPEQKGALLKFLQM
                     LTNWDISLFHYRAHGADYGDILAAFALNRGDEVELNRHLEQLGYRFQNVSESPAYQYF
                     LK"
     gene            13746..15080
                     /gene="glmM"
                     /locus_tag="PWY43_000245"
     CDS             13746..15080
                     /gene="glmM"
                     /locus_tag="PWY43_000245"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2135163.1"
                     /translation="MARKYFGTDGVRGEVGKFPITPEFALKLGWAAGKVLATQGTKKV
                     LIGKDTRISGYMLESALEAGLTAAGLSAVFVGPMPTPAIAYLTRTFRAEAGIVISASH
                     NPYYDNGIKFFSSVGEKLPDEIEEAIEAMLDKPMDCVDSANLGRASRINDAAGRYIEF
                     CKGTFPSELSLEGYKIVVDCANGATYHIAPNVMRELGAEVLEIGTKPDGLNINEKCGA
                     TDIKALQKVVVEAGADVGLAYDGDGDRLIMVDHLGNKVDGDQILFILAREALRSGKLQ
                     GGVVGTLMSNMSLELALKELAIPFSRANVGDRYVLEQLKEKGWKLGGENSGHIIVLDK
                     NTTGDGIIASLQVLAAMASHKLSLNDLAKAVPLFPQVLLNVRFEKGAANPLESNEVKA
                     VAADVEKRLAGKGRILLRKSGTEPLIRVMVECEDAVLAQSCAEEIVEAVKRN"
     gene            15225..15884
                     /gene="rpiA"
                     /locus_tag="PWY43_000246"
     CDS             15225..15884
                     /gene="rpiA"
                     /locus_tag="PWY43_000246"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL2135164.1"
                     /translation="MDQQEMKKIAAQAALKFVKSDTIVGVGSGSTVNCFIDALASMKD
                     EIKGAVAASKASEQRLRDIGIEVFSANEVTELDVYIDGADEITPQGAMIKGGGAALTR
                     EKIVSSLAKKFICIVDKSKQVDVLGSTFPLPVEVIPMARSYVARQLVALGGSPEYREG
                     VVTDNGNVILDVHNFKIMEPLKMEHTINNIAGVVTNGIFAQRFANVTIVGTPEGAVIL
                     E"
     gene            15921..17150
                     /gene="serA"
                     /locus_tag="PWY43_000247"
     CDS             15921..17150
                     /gene="serA"
                     /locus_tag="PWY43_000247"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL2135165.1"
                     /translation="MAKISLDKSKIKFLLLEGVHQNAIDVLLAAGYTNIEVHKKALDG
                     QELIDAIKDAHFVGIRSRTFLTEEVLAQAPKLIAIGCFCIGTNQVDLNAAKRRGIPVF
                     NAPFSNTRSVAELVLAEMILLMRKVPTANAEVHRGVWNKSAAGANEVRGKKLGIVGYG
                     HIGSQLSILAEAIGMQVYFYDVENKLPLGNAQQVPTLEHLLSTCDMISLHVPEIDSTK
                     NLMSAERIAQLKEGSVLINAARGTVVDLDALAARIEDGSLRGAAIDVFPEEPASINDP
                     FVSPLCKFDNVILTPHIGGSTSEAQANIGTEVANKFVKYSDNGSTVTAVNFPEVSLPL
                     HTNTKRLLHIHENRPGMLNQINQIFVESNVNIAAQYLQTDAAIGYVVIDVESDNVDEA
                     LQRLKAIEGTIRARVLY"
     gene            complement(17228..17746)
                     /locus_tag="PWY43_000248"
     CDS             complement(17228..17746)
                     /locus_tag="PWY43_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDL2135166.1"
                     /translation="MKKLTIVALTCLFSTHLLAAPVGNTFTGIGVGVDVTTTKYKVEG
                     VKGKDSTGPALVVDYGIEQGNNFVGVVQGKAKVGSTKVFSDVKQKNKYTIAYQQGYRV
                     GSDLLPYVKVDASSSKIGNETYRGFGFGAGAKYAVSSDVELGAEYTRDNLKRSGTKLK
                     GNAFTANATYRF"
     gene            complement(17766..18488)
                     /gene="arcA"
                     /locus_tag="PWY43_000249"
     CDS             complement(17766..18488)
                     /gene="arcA"
                     /locus_tag="PWY43_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDL2135167.1"
                     /translation="MKIPQILIVEDELVTRNMLKSIFEGEGYQVFQASNGEEMHSVLS
                     TNEINLVLMDINLPGKNGLILAREIRENMKLPLMFLTGRDNEIDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLQRTMIDKENDVVKEQSEIESYRFNGWILDINSHSLISPEGEV
                     NKLPRSEFRALLHFCQHPGKIQTREDLLKKMTGRELKPHDRTVDVTIRRIRKHFEDHP
                     NTPEIIVTIHGEGYRFCGNIEP"
     gene            complement(18621..19313)
                     /locus_tag="PWY43_000250"
     CDS             complement(18621..19313)
                     /locus_tag="PWY43_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2135168.1"
                     /translation="MILFAGDPHGSYEHLYPILQQQTEEVALVILGDLQLTTPDELDR
                     LSQYCDIWFIHGNHDSKTVAAFDSIWNSSWKERNIHGKVVDIQGIKIAGIGGVFRGHI
                     WMPPNRPMFFDPIHFCQYSAPEKIWRGGIPLRHRSSIFPSDFEALENEKADILITHEA
                     PKPHPQGFSVINQLARKLGASKIFHGHYHDNFNYGNLNVSRECAIYNIGFRSLADING
                     NYLILGVDDREK"
     gene            complement(19331..20605)
                     /gene="nadR"
                     /locus_tag="PWY43_000251"
     CDS             complement(19331..20605)
                     /gene="nadR"
                     /locus_tag="PWY43_000251"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDL2135169.1"
                     /translation="MTDFAYLQQKRKQLKLKVNDICEQAGVTRAYFNQLVSGKIKNPS
                     ANKLKALHSVLDIVEDSNQRSGVIFGKFYPIHTGHINMIYEAFSKVDVLHVIVCTDSE
                     RDLRLFQESKMKRMPTNEDRLRWVQQIFKYQQKQIFIHHLVEDGIPSYPNGWEDWSGR
                     VKALFEEKSINPTIVFSSEIQDKEPYEKYLHLEVQLVDPTRESFNISATQIRNNPFQY
                     WRFIPKEVRPFFVKTIAILGGESSGKSVLVSKLANVFNTTSAWEYGREFVFEQLGGNE
                     QAMQYSDYPQMVLGHQRYVDYAMKHAHKVAIIDTDYITTQAFCIQYEGKAHPFLDSMI
                     REYPFDVTILLSNNTKWVADGLRSLGSKKQRQQFQHLLKKLLEKHHIPYIKIESPSYL
                     DRYNQAKEVVEAILNEEPIPLQFKQYNIHEFE"
     gene            20829..21791
                     /gene="ribF"
                     /locus_tag="PWY43_000252"
     CDS             20829..21791
                     /gene="ribF"
                     /locus_tag="PWY43_000252"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDL2135170.1"
                     /translation="MQLIRGFHNLDKHIALNQGCVLSIGNFDGVHLGHQNILARLCDR
                     ALDLGLPSVVMLFEPQPREFFAKKSGNSTACNPPARLMRLRDKLKYLEEAGVDFVLCV
                     RFSEKFAQISADDFISDLLAKRLKVRYLSVGDDFRFGAKRAGSFETLRNAGLKYRFAV
                     EESHTHSLNEERISSSLVREALQNNELDLAEKLLGKPYSILGRVAHGNKLGRTIGFPT
                     ANIMLNRLVTPIQEVFVVKVKTKQGSFNGIANVGNRPTINGTKPLLEVHIFDFNASIY
                     GEAIEVIFLKKLRNEIKFPDFEALKAQIEKDRLQAVEFFKEFAR"
     gene            21894..24710
                     /gene="ileS"
                     /locus_tag="PWY43_000253"
     CDS             21894..24710
                     /gene="ileS"
                     /locus_tag="PWY43_000253"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL2135171.1"
                     /translation="MTIDYKNTLNLPETGFPMRGDLAKREPDMLKNWYDKNLYQKIRT
                     ASKGKKTFILHDGPPYANGNIHLGHAVNKILKDMILKSKTALGFDTPYVPGWDCHGLP
                     IELKVEGIVGKPNEKISAAEFRQACRDYAKEQVEGQKADFIRMGILGDWDNPYLTMNF
                     NTEANIIRTLGKVIANGHLYKGSKPVHWCLDCGSSLAEAEVEYEDKVSPSIYVRFNAV
                     DVNAVEEKFNAQGKGRGALSAVIWTTTPWTIPSNRAIAINPELDYALVQLGDERVLLA
                     VDLVEDVAKAAGVESAEILATTKGENLELLRFNHPFYDYSVPFIFGDHVTTDGGTGLV
                     HTAPDHGADDFVVARKYNIEMAGLISNDGKFKADTPFFAGLGVFESNDKVVEKLQEVG
                     ALLKLSRIKHSYPHCWRHKTPIIFRATPQWFIGMETQGLRQQALGEIKSVRWIPSWGE
                     ARIDTMVANRPDWCISRQRTWGVPMAMFVHNETEELHPRTLELIEEVAKRVEEKGIQA
                     WWDLDPAELLGEEAKDYRKVPDTLDVWFDSGSTYASVVEQRPEFNGNLADMYLEGSDQ
                     HRGWFMSSLMLSTATNGKAPYKQVLTHGFTVDEKGRKMSKSLGNVIVPSEVWNKNGAD
                     ILRLWVASTDYTGEITVSHNILNSAGESYRRIRNTARFLLANLNGFDPKSDLVQPQEM
                     IALDRWAVACALEAQNDIKEAYDNYQFHTVVQRLMRFCSIEMGSFYLDIIKDRQYTTK
                     ADSLARRSCQTALWHIAEALVRWIAPILSFTADEIWGYLPQVEGRSEFVFTEEFYTGL
                     FGLTEQDKLDDAYWQQILKVRAEVNRVLEQARKDKVIGAGLEAKVTVFANDEIRPLLE
                     QLGNELRFVLITSQAIVKPLAEADIAEGELAGLAVKVENADGEKCPRCWHYATDIGSH
                     SGHEEVCGRCVENVAGEGEKRLFA"
     gene            24835..26382
                     /locus_tag="PWY43_000254"
     CDS             24835..26382
                     /locus_tag="PWY43_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HDL2135172.1"
                     /translation="MMKIIAFLFIFLSVFSHTALANSMAEQCVALKSNSPEKTAIYKA
                     EWVEKSAELPAYCLVQGEIGKRVGVDNVPYGIQFELRLPEQWNEKFLFQGAGGVGGVI
                     FPAIGKLYPHGASGANGLNRGYAVVSNDSGHATRDLVFTADPEARSNYAYASIGKVTT
                     VAKQLIAEFYRKPSKHNYIMGCSNGGREAMMAAMRYPQEFDGVIAGSPGFRVSRSVLA
                     EVWDNRALLAVAPKNGDGDKILSQALTQQDLDVIANGVLTRCDKLDGLADGLINAWEQ
                     CDFQPEMVAKQLGQKKVALIKTIFEGAKNSRGELIYSPWAYDSGINAKGWRDWKLGDS
                     QTAQPNSISFKMGLKSLTHYYLAPRVPSRDPLSVDLDQASEQVKAVGSIHDADQVDLS
                     AFKQNGGKMLIYQGVSDPIFSAVDLKNWYQSLQQAVENPQHFAKLFFVSAMNHCGRGA
                     TVNDFDMLTALENWVEKGLEPESIAAKAGELYPNKAKQIPLCAYPKVAIYQTGEDPNR
                     LTSYQCR"
     gene            26452..27819
                     /gene="pssA"
                     /locus_tag="PWY43_000255"
     CDS             26452..27819
                     /gene="pssA"
                     /locus_tag="PWY43_000255"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDL2135173.1"
                     /translation="MLILNKQRKAEYLLKSLPYLPQVAEQVQFLASSKAFKQQILQLI
                     QTAKKRIYITALYFEKDEAGQEVLAALYQAKRNNPELEINILVDWHRAQRGRIGEAEN
                     LSNADWYAKVKYEQGLPAGQEIAFYGVSINGREVFGVLHLKGFVFDDTILYSGASINN
                     VYLHQFERYRYDRYHVIENKVLADSWVTFIQQHLLTNEAVKRLDTENRPKTAEIRAQI
                     KHFRKQLSREAYLFNGVAENGRLAVSPLVGLGRRKNQLNKVIEALFYQTEQKLTICTP
                     YFNFPHSLRTRIEWLLANGKSVEIIVGDKTANDFYTKPDEKFTMASALPYLYEKNLRA
                     FAKRLDGYVQNKQLTIRLWKDGENSYHLKGVWVDNRYILLTGNNLNPRAWRLDAENAI
                     LISDPQHQLSEKAETELNQICQHTQILTHYSDLEVLTDYPENVRKLLKKFGRVKLDKI
                     VKMLL"
     gene            27874..28617
                     /gene="rlmB"
                     /locus_tag="PWY43_000256"
     CDS             27874..28617
                     /gene="rlmB"
                     /locus_tag="PWY43_000256"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL2135174.1"
                     /translation="MSEQIYGIHAVKAFWDNAPERLIEVLVLKGREDKRLMPLLNELQ
                     RLGVAIQQVNRQTLDNKSQGEVHQGIIARVVPQKELNEHDLEAILSQKKNPLLLILDG
                     VTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTPTARKVACGAAEVMPLIRVTNLART
                     MRDLQERHNVWIVGTAGEAESGIYEAKLTGSIALVMGAEGDGMRRLTREHCDQLISIP
                     MAGSVSSLNVSVATGVCLFEIVRQKLAGQ"
     gene            complement(28704..30116)
                     /locus_tag="PWY43_000257"
     CDS             complement(28704..30116)
                     /locus_tag="PWY43_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2135175.1"
                     /translation="MKLLNIQNLSVETENRLQLVEPISLQLKAGKNITILGETGSGKS
                     LLIQAIMGALPDGLIASGEISVKNCKISTESDRLQTLWGKTLVMLPQEPRRSLNPIMA
                     IGKQLWESFHYVAGKPSSQAKTESKAYLAKLGLGESVENYPHQLSGGMAQRASFAIAT
                     AAGGKILLADEPTKGLDPTSKGKVIELLKSAYQNGGGLLTITHDIEVAEQLGGTILVM
                     KKGELLEQGEAKALLNNPQHPYTKALISADPKHWQPLQDLQNLSQKQPLVSVKNLSIA
                     RGKKTLFSNLSFDLHKGEVLGIVGHSGIGKSSLADVLCGLLKPKSGEVVWHSHSHKKH
                     QVLKLYQDPPEAFAPNVSLKTLLDDVINKHQLDRLPIPSLLQQLSLNPEILQRNAENV
                     SGGELQRVAILRALLLEPVLLFADEVTSRLDPITQQETIELLINQCRARNCSLILVSH
                     DPYLIEKSCDKVINLEEYVG"
     gene            complement(30205..30987)
                     /locus_tag="PWY43_000258"
     CDS             complement(30205..30987)
                     /locus_tag="PWY43_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2135176.1"
                     /translation="MKISISQYLGGTILLAMLGFAFLQPYFYPMDIGFQDLTNILSNP
                     NEIAWFGTDHLGRDMLARLASAIRLSFGLSLFSVICALIAGLITGIMAGFFGGWIDRI
                     FSFICDLVMALPGLLLVLLFAALSPGSFWTLYLGISLVMWVEFFRVIRSISSTMANSS
                     EIQASRLMGMGWFYSFKRHLLPRLLPMIATLSAFSLGNAILALATLGFVSVGLRPPTA
                     ELGLMMTELFPYYYEAPLIFLQPVVAVFLMVLSLQLLSRKIK"
     gene            complement(30997..31929)
                     /locus_tag="PWY43_000259"
     CDS             complement(30997..31929)
                     /locus_tag="PWY43_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2135177.1"
                     /translation="MHKILFRRLIQILLVIWSVGTLTFILTRQLSGDMAYRIAASRYG
                     YDQADSAAAEAVRAELGLDQPWWQSYFSWLSDLAQFNLGKSLVTGDPVWSEIQHQFGH
                     TLSLAIVALILAILIGPPLGLLAARKPNGFFDRLTLVFSTLFRSVPAFIIAIGLITLF
                     SAQLKWLPAGGYGSWQHFILPALTLAIGLSTVSVRVTRSAMVQVKGSEFYQFSQLKGL
                     SRWHTFLRHGLPNIAVPVIAYHAVQLVYLIEGVVVVESLFAWPGSGHALVHAIIARDV
                     PMIQGTTLVMGGLFVVLNTIADLLSAWIDPRIKR"
     gene            complement(32149..33723)
                     /locus_tag="PWY43_000260"
     CDS             complement(32149..33723)
                     /locus_tag="PWY43_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2135178.1"
                     /translation="MKKWLIPLTVAAFCVSACDDKNSTQATAEKPQVENTQSQKNAEI
                     LPLATVVAPWEINSLDPNQSGVIFQRMNLAETLVEADLNGNLIPALATDWRSNEEGTI
                     WRFTLREGVKSHNDQVLTAQAVVNSLNIAKSKPGVLAKAFIKEIRAVDNKVEIELTKA
                     FVPFPSFLTHYTALILAPESYNEKGEVTQIIGTGAFKPTKIEAPQKLEAVQFEGYWGK
                     KPQVQQANYLASSRSETRMLMAQSEPSSLVFNLDTASVARLKADPNLQLVSGSIARTI
                     QLKMDVAKPFFNDLAIRQALSQAINRKAIAEQVLKIENGAADQILPKAFADWRVDTAE
                     QVVDIAKIKQNLTASGYTFDEQGRLTKDGKPFSFTMRTFSDRPELPVIATILQAQWKQ
                     IGVEVNVSVGNFSEIPAGHQDGSLEMALYALNYGKTLDPYGVIASDFANGGSDWGVMN
                     WQNTEVDNAVKSLETERDPQKAKALKQTVSKIIYNELPIIPVVYYQQNVVAHKEVKNV
                     TLDPFERRFFLELLSK"
     gene            complement(33981..35561)
                     /gene="prfC"
                     /locus_tag="PWY43_000261"
     CDS             complement(33981..35561)
                     /gene="prfC"
                     /locus_tag="PWY43_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL2135179.1"
                     /translation="MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVK
                     GKGSAQHAKSDWMEMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTA
                     VDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
                     IKCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEVRVVKGLNNPELDQAVGD
                     DLAQQLRDELELVQGASHEFDHAEFIKGELTPVFFGTALGNFGVNHFLDGLTEWAPAP
                     QARQSDEREVSPSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIG
                     KDVVISDALTFMAGDRSQAEEAYAGDIIGLHNHGTIQIGDTFTQGENFKFTGIPNFAP
                     ELFRRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLINNDLIVGAVGVLQFDVVVSRL
                     KTEYGVEAIYETVNVATARWVEGKDAKKFEEFKRKNEANLALDGGDNLTYIAPTMVNL
                     NLAQERYPDVQFFKTREH"
     gene            35794..36669
                     /locus_tag="PWY43_000262"
     CDS             35794..36669
                     /locus_tag="PWY43_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2135180.1"
                     /translation="MMKKFIPVMIAALVLSACSSQPAKRKSTPRIKATAAYIPTENSA
                     YVVFDLNQKKFLEGKNFNLVRPIASVTKLMTAVVFLEENRYGASCQTQILPSDADFLK
                     GTTSPLPKNLNIACSELLKAMLVRSDNYAAHALAHATHLNKAQFLARMNSKAREIGMT
                     QTYFGDSSGLSSDNVSTVVDLAKLAGYAASKPEIQQLSNSPLVSVYAGGRYFSMKNTN
                     SMVRSQSYQALVSKTGYTREAGYNLAFIAKGDCRGKRIGVVSLNNDNAASRTDFTESM
                     LAKYQCVPNPYRQYD"
     gene            36742..37329
                     /gene="pth"
                     /locus_tag="PWY43_000263"
     CDS             36742..37329
                     /gene="pth"
                     /locus_tag="PWY43_000263"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL2135181.1"
                     /translation="MSEIKLIVGLANPGVKYEETRHNAGEWLINELARIYNVSLKEEP
                     KYFGKVAKINTAQGEVRLLVPTTFMNLSGKAVGALANFYRIKPEEILVAHDELDLPPG
                     SVKLKKGGGHGGHNGLKDIIAALGNNKEFYRVRIGIGHPGHKDLVANYVLGKPSPSDQ
                     TLLNSAVDEAARCVEVLFKDGMVKATNRLNSFKAN"
BASE COUNT        11060 a         7247 c         8392 g        10644 t
ORIGIN      
        1 tatgttcttc tctttatatt ttactaaata gtatagcaaa attaatataa agaaataaca
       61 cattgaccct gaattatgag ctaagaatga ttggcttaag aagaatgaat gagaaataat
      121 atgtattatc cctaaaatgg cagtgcattt tattgctata ttattatctt tcaagcgttt
      181 tgtaaaaatg atgaaaggaa tgattaaaac tgctattaat gctaaaaatc caataaggcc
      241 acgctttaca gaagcttcta gatattgatt atgtaagctg ttaaagtacg ttgtttttgc
      301 cattttattt gctttcattt gtctttctct gaaattatca tatccgatac caccatgtcc
      361 taatattggt ttttctgtaa ttgcagctat ggtgttttcc cacatatcaa aacgggcacc
      421 gagagaggaa tttttatttc cttgctcaaa atagccgatt atatctgatt tagcctcatt
      481 atatctttgt tgaataccaa attctggtct agctataaat atagctaata caacacttaa
      541 taataaagag atgaatatga ttagtttttt atttatatgt tgtaaattgt atattagaat
      601 gagtagaaaa caaaaaggaa aggcaatcca gccacccctt gcaccgctta gaatgctggc
      661 taacaaacca aatgcagcga agataatata taagagtgct attctatttt tcttttctgc
      721 gaactaataa aatgcaatgg atatactgag taatcctaat aatatagaga tattccccgc
      781 ttgaatatgc attatgtcag ggaatggttt ttgccattta agaataaatt tttgataaag
      841 agcaagcatc cccaccaaga atgaaccaat aggaataaaa tgaaaaatta tctctttttt
      901 aatagagtaa atattaaaaa agaaaattaa tggaattagt aatagaattc tacttggatt
      961 atcaatttct ctaaatccat caccattgaa aatagttgaa atcacaaatg ataggaaata
     1021 agcaataaga gtaaaaataa aatatttatc ctccttatcc aactgccatt ttattttcaa
     1081 tttagatctg taatataaga aactaaatgt tgcaataacg cctaaagtca tcggcacata
     1141 actatatcct tttttaaagg tcagcactgt tacaaaaaat gcagcaacta agatatttac
     1201 aaaaagcgtt atttgtggct tttctatttt tttcagatga tgaagcatta aactaagtcc
     1261 taaagaatgg aaaattcttg ccattttata ctaaggctat agctagtatc tatcatttta
     1321 gcaaagtttt gttgaatttg agggattatt ctgcataata tccttatgtt tctgaaaaag
     1381 gaggcggtat gagcttatta ccaatgaacg aaattaccag cttaaatcaa ctggacttac
     1441 aaaaaagtta cagttacgct gattatttgc tatggaaatt taaagagcgg gttgagatta
     1501 tcaaagggaa aattgtggcg atgtctccgg ctcctactcg ttttcatcag cgaatttcta
     1561 tgaagatgac agcaaaattt ttagaggttt ttgacaatca ccaatgccag atttattctg
     1621 cgccttttga cgttcggttt cccgatgaaa acggaaaagt caaaactgta gtgcagcctg
     1681 atctttgtgt aatttgtgat gtcagtaagt tagatgaaag aggctgtgta ggggcaccgg
     1741 atttagtggt ggaaatcctt tctccgggta ataccaaaag ggagatgaaa gataaatacg
     1801 agctttacca ggaacaaggg gtgaaagagt attggattgt ttctcccgaa acccgcacta
     1861 ttcagatttt tgtgttggaa aacggtaaat atatcggtat tcagccggtg gcagaagatg
     1921 aaccggcaac ttcggtggtc tttccggcat taagtttttc gaccgagaaa ttattcgaac
     1981 tgtagaaaaa tgcaacataa tacggcggtt ttatgccaca atactacttt acttatgtga
     2041 gtgcttaagg tagaatgcac tacctttttt gttctatgcc gccaaaattg tgtgcggttt
     2101 aatttaatca tatttttctg aggtgatggc atatgccagt aattaaagtt cgtgaaaatg
     2161 aatcatttga cgtagcatta cgtcgtttca aacgctcttg cgaaaaagcg ggtatcttag
     2221 ctgaagttcg tgctcgtgaa ttctacgaaa aaccaactac aattcgtaaa cgtgaaaaag
     2281 catcacttgc taaacgtcac gctaaacgta atttccgtga gaacgcacgt aacacacgtt
     2341 tatactaatt agttaggctg gttagcaaac tatatcgaaa ccgtgattcc gttaggctca
     2401 cggttttggt gtttttaacg tttctatttc aagcggttgt tttttgcaaa aaatttgcaa
     2461 aattgctccg cttgtatcat tcaaagagca ggaggaaatc aatgaaaggc acaattccac
     2521 gttcatttat tgatgatctt atcgctcgca ccgatattgt tgaggtcatc aacagtcggg
     2581 tcaaactcaa aaaggctggg cgagattatc aagcctgctg tccttttcat cacgagaaaa
     2641 gcccttcctt ttcggtaagc caatcaaaac agttttacca ttgtttcggc tgtggcgtac
     2701 acggcaatgt catcaccttt ttgatggatt atgacaaact cgaatttccg gaagcgattg
     2761 aagaactggc agctatgcac gggctggaag tgccgagaga gaatgttatt caacgcgacg
     2821 gcaagcctca agccaatttt aaaaccaagc gtaatcttta tgaattgatg gaggcggtgg
     2881 cgaagttcta tcagcagaat ttatcgcacc aaattgagcc acaaagctat ttacagcaaa
     2941 gaggtttgtc gcctgagatt atcgaacgct ttgaaatcgg ttttgctccg aatgcctttg
     3001 atgcagccct ccgtactttt gggcaatcgc aggaagatat tcagaaattg ctcgatacgg
     3061 gcgttttaac caaaaatgat agcggtcgaa tttacgataa atttcgcaat cgggtaatgt
     3121 tcccgattcg ggataagcgt ggacgagtga ttgcctttgg tgggcgagtg ctgccgaatg
     3181 atgagcaagg agcgaaatat atgaactcgc ccgaaacggt gatttatcat aaaggtagcg
     3241 agttatatgg cttgtatcaa gcactgaaag tcaatgaaac gccggattat ctattcgtgg
     3301 tggaaggata tatggacgtg gttgcccttg ctcaatttgg ggtggataac gcagttgctt
     3361 ctttaggcac ggcaaccact ggtgaacaaa ttcagcaaat gtttcgttgc accgagcaaa
     3421 ttgtctgttg ttatgatggc gatagagccg gccgagatgc tgcatggcgt gcattagaaa
     3481 atgcgttgcc ttatttgcaa gacggtcgcc aactgaaatt tattttcttg cccgatggcg
     3541 aagatccaga cacctttgtt cgttctcaag gtaaagaagg atttgagcaa tatgtggcac
     3601 agaatgccaa gccattaaac gatttcttat ttgacagttt gctggcggat gtggatctct
     3661 cttcaaaaga gggaaaatcc aagctcgctg ctttagctgt gccgttaatt aatcgtattc
     3721 cgggcgagat gctgcgagtg tatttacgca atattttagg acagaaatta ggcattttag
     3781 acccaacgca gatagaagct ctgttaccga cgaaaaaagc ggcagagaag cctttacatc
     3841 aagcggtcaa aatgaaacgt acgccaatgc ggctgttgat tgcgttattg attcaaaatc
     3901 cgtatttggt gaaattcgta cccgatgtaa gtgctttagc ctccttgaaa gatcctggct
     3961 ttacattatt aaccgatatt gtcgcaactt gtcagcaaaa tgcaggtatc agtatgggcg
     4021 ggctacttga acattatcgg gaagatcccc aatataagat tgttgaagcc ctcgcaaatt
     4081 gggatcattt agagttaccc gaaaatatcg aacgaacatt tatagacact ctcgattttc
     4141 tctacgccaa attagtggaa ttacgcaaag aagagctaat tgccaaagac cgaactgtgg
     4201 gcttaaccga tgatgagaaa aaagagtatg ctcttttgat gtttcaatag aattttaagc
     4261 tcgaataatt cccctcttta gcaaagaggg gctaggggag atttggcaga agtgatgaac
     4321 gaagaaaata attaattcta tctaaaaaga aagttattta tctctcttgc tacacctctc
     4381 tgttctcata agcatcaaag taatgtgtaa attagaaaaa tagaattttt ttgtgaattt
     4441 atgaagtcga accaaattta gtggtataat ccactactat ttttgctcgc ataataaacg
     4501 gatagactat ggaaaaccaa gcagatcaac aattagaaca gcaatctcaa ttagaacttc
     4561 tgatcagtca agggcgtgaa cagggatatt taacgctttc tgaagtgcac gaccacttac
     4621 cggaagagtt ggtggatgcg gagcagatcg aagatattat tcagatgatc aacgatatgg
     4681 gtatccaagt tttagaggct gcccctgata cggatgattt actactcgct gaaaatatcg
     4741 ccgatgaaga tgtggtagaa gaagccacca aagtgctttc aaccgtagaa tcagagttag
     4801 gtagaactac tgaccctgtg cgtatgtata tgcgtgagat ggggacagtt gagcttttaa
     4861 cccgtgaagg tgagattgat atcgctaaac gcattgaaga agggattaac gaagttcaat
     4921 gtgcgattgc ggaatatccg gaagccttaa gtggcctgtt agcgtgctat gctcaagttg
     4981 aaaccggtga aatgcgcctt tctgatttag tgacagcatt tgtcgatcct aatattttag
     5041 aagaagcacc gttagaggat attgaggctg ctttaggtga agatgaagaa tcttctgatg
     5101 tgcctgatgt agatgacgaa aacgaagatg aagacggtga tagcagctca gacagcagtg
     5161 atgacagctc aattgacccg gaacttgccc gtgaaaaatt tgaagcccta cgcagccaac
     5221 atgaaaaagt gttattggtc attcaaaaac acggacgaac cagtaaaaaa tcgaaagagc
     5281 aaattgaaat tttatctcat attttccgtg agttccgttt ggtgtcgaag caatttgact
     5341 tattggttgc ttctatgcaa aaactgatga agcaggttcg cactgaagaa cgtcaaattc
     5401 aacgctatgc agtggaatat gccggcatga aaaaagcgga tttcttgaaa gcattccaag
     5461 ggcacgaatt aagcgaagcg tggattgaaa aagcgattaa tgccaagaaa aatgcagtac
     5521 cgaaattagc taattatgtg gataatatcc gtgtcaatat tgctaactta cagaatttag
     5581 agcaaacatc aggcttaacc attgctcaaa ttcgtgaaat tggcgggcgt atttccaatg
     5641 gtgagttaaa agcccgccgt gcgaaaaaag agatggtgga agctaactta cgtttggtga
     5701 tttcgattgc gaaaaaatac actaaccgtg gcttgcagtt cttagattta atccaagaag
     5761 gaaacatcgg tttaatgaaa gcggtggata aatttgaata ccgccgtggt tataaattct
     5821 cgacttatgc aacttggtgg attcgtcaag cgattacccg ttcgattgct gaccaagccc
     5881 gtacaattcg tattccggtg catatgattg aaactatcaa caaactcaac cgtatttcac
     5941 gccaatgttt acaagaaatg gggcgtgagg caacgccgga agaactggca gaacgtatgg
     6001 gaatgccgga agataaaatc cgcaaagtat tgaaaattgc caaagagccg atttcaatgg
     6061 aaacaccaat cggtgacgat gacgattcac atttaggtga tttcattcaa gacgaaagcc
     6121 ttgaattacc attggattcc gccactgcgg agagcctgcg tatggcaacg aacgaagtgt
     6181 tagagggctt aaccccgcgt gaagcgaaag tgttgcgtat gcgttttggt attgatatga
     6241 atacagacca cacactcgaa gaagttggta aacaatttga cgtaactcgt gaacgtatcc
     6301 gtcaaattga agcgaaagca ctgcgcaaac ttcgccaccc gagccgttcg gaaaccttac
     6361 gcagtttctt agacgactaa tcatttcccc tatgcctgat ggtatagggg attttgtttt
     6421 aatctgtata acaagcggtg atatttaccg tattttttac aaaagaggga gagatgaaaa
     6481 aatcactatt agcactttta attggctcag tatttctgat ttcagcttgt gaagacggca
     6541 aaatgaccca aacggtatta gaagccgaaa aacaaattgt tcagttaggc acagaattaa
     6601 aaaatgctca ggctgattta gcaaaaaaag aggctgaagt tgcagaactt tcaggggtga
     6661 aagcagaaaa tgaaaaatta aaatcagagt tagagaacgc tcagcaaaac acagcgttaa
     6721 atgttgaaat tgtgaaaatc tttgataaga aagaggtgat taagcaccaa gttgatccga
     6781 aacaagaatt tgcaatagaa gaaagtacag taagtgcgtt tgtgtctcta ccaaaaacca
     6841 attttgattg gcttaaccag cttttgatta atcaggccta tgattacaat gaagaacaag
     6901 gcagaaagct gaaaaatcca accgaagaag agtttaaaaa gcatcgtgaa gcggtttatc
     6961 aagggttggt agaaagtgtg aaaaccgagc cagctattgg ctatgatgat catatttcca
     7021 gtgcttttat cgggcaacgc aataatattg ctacattcac tatgatgcat tacacctata
     7081 tgggtggggc tcacggtatg cactatacgc aatatatcaa tgtagattta gctaaaaaag
     7141 caatcattgg cttaaatgat ttaatcagcc caaaaaatca agttcaactg aaagacattc
     7201 tttgggaaaa ttacagtagc gtgcgtgtgg atgaaaatgg caactataat ggatttgctg
     7261 ataaaaaaga gttccgtatt tctgaccaat tctactttac ccctcacggc attgcttttg
     7321 tttatccgcc atacgaatta ggtgcttatg cagaaggcga tatagaggtt gaagcggatt
     7381 ggtacattat taataagttg ctcaattcgg agtatcaacg tggcgaaaaa gatggattct
     7441 ttgaagaact agataactaa ttgtaaattt agataaaaaa caaccgcttg taatccttta
     7501 ttacaagcgg tttagttttg ttaaaatttt gcaacttacc ctaacacaat gcccaaacta
     7561 aataatagat tagtgagcaa cgccagccct gccatttgcc ctagaattgg gtagagttct
     7621 tttgggtctt tatgctgata gacaaaacga gcgtgttttg ccagtaacgg tacagacagc
     7681 aaaaaaatga agctatacca atgttcaaat tcagaaattg caaaaatcag ataagaaatg
     7741 accgctagca ctaacaatgc aacgtggtat tttcgcccgt tttggctacc gattcgtaca
     7801 atgagagtgt ttttcccagc ttgtcggtct tggttaatat cacgcaagtt attgatattt
     7861 aacactgcta cagacagcaa tccgcaaccg aaagcaggga gcagaatcat tataggtagg
     7921 ctatgagcct gcaagtagaa cacgccgatt accgctacaa agccaaagaa aatcagcaca
     7981 aatagatcgc ccagccccaa atagccgtag gcttttttgc cgacggtgta agtaatggcg
     8041 gcaataattg agacgatgcc aagcccgata aagaaaaaca gatcctgcac tgtttcaaaa
     8101 gcataggcac ttaaggcaac accggaaaca aacgagagta ggcttaagat aatcacggct
     8161 ttcttaagtt gttcaccgct aataacgccg tgttgaatcg cccgtagcgg tccgattcgt
     8221 tctttggtgt cagagccttt gacgtggtcg ccgtaatcat tggcgaagtt agacaggatc
     8281 tgcaacaaaa tggtggtgat aaatgcgagt aaggtggtaa caacatcaaa tttccctgcc
     8341 caatgagcaa gtgctgaacc cacaataatt gaagctaacg ccagcggtaa agttttcggg
     8401 cgagcggtgg taaaccaaat cgaaaagaga gaatttttgt tcatagagaa taatcttagc
     8461 gtaatttcat ttacaatgat gttaaggatt taagcgatag tgcaagcgtc cactcttgtg
     8521 ttaaatggta aaacgaattg gtacaagcgt ggacgcttgc accatcctca atttcatatc
     8581 attatgtcca atattttata ttattttaac agaaaaaagc gtaatgaact ctacctcaaa
     8641 tcaactaacc ccgattcaat taaaccctat cggttttgtg gaaagcccct atgatgaaaa
     8701 attttccgtg cctcgtcagc cgaatttggt agcggagggg aaaggcattc tacgtttaat
     8761 tccgccttac aatacccccg atgcagtcag aggcttggag aaatttagtc atatttggtt
     8821 gattttccaa ttccatcaca ttcccgaaag agagtggcac gccaccgtca gaccgcctcg
     8881 cttgggcggg aacgaacggg tcggggtctt tgccagccgt gccacccatc gtccgaatcc
     8941 gcttggctta tcgaaagtga agttagaagc ggtagtggcg ggggggagtg aagtttattt
     9001 gaaattaggc agcgtggatt tagttaacgg cacaccaatt tttgacatca agccttacat
     9061 tgcatttgca gatagcgagc cgcaagcggt ttccggtttt gctcagcaaa aaccacaagc
     9121 aagattagcg gtgcgtttta gcgaggaggc aaaacaagct gtcgaatttt gccgaaattt
     9181 tgcaaaattt ggcatcgaac agccgcttag ttttcttgcc gatgtcatcg cccaagaccc
     9241 tcgcccggcg tatcaacaag ggaaacctag tgagcgagtt tatggtatga accttgccgg
     9301 ctacaatatt cgctggcaaa ttgaaaatga tacacaggcg attgtgcttt cgattgaatg
     9361 aactttccta cgcaagtgag atctaagaaa tgatttttaa cccttttaag gactgattat
     9421 gaaattgaaa aattttctta ccattctacc gcttgcagtc gcaacagcgg taacttcagc
     9481 ggaaactcat accgtggaac ataaagataa accggcttct tttaatttct caaccgaagt
     9541 ctctcgcacg gtggaaaagg acgtgatgca ggcaagtgtg tatagccaaa aatcaggtaa
     9601 atccttgccg gagctgaaaa aatttgtatc tgaaaacctg aatcgtgtat tggctgaggc
     9661 aaaaaaacat ccatcgattg aagtacaagc tgaaggtgtg actaatcacg ttaattataa
     9721 caacaaaggg aatattgatg gttgggatac ctctggtagc attcagttga aaagcaaaga
     9781 ttttgatgca atggcagcgg tgttggaaaa tttaggtaaa gatgtggcga ttcgttacat
     9841 tgattttagt gtatcgccag agaaaatggc gtcccttgaa gatgaaatga cgctggaaat
     9901 tatcagcaaa tttcagcata aagccgatgt gattcaaaaa gccttagggg cgaaaacata
     9961 taagctagtt aatatcaata ttcaaactcc aaatgatggt ggtagaccat attatgaaca
    10021 cagagcaatg tatgcagttt catcaatggc acaaagcaaa tctatggatg aaatcccgtt
    10081 agaagcaggt aaaaccacaa tttcagctac cgcttcagga agtgtggaat ttgaatagaa
    10141 atgtatagat ttgtatagag ttgtattttt actttaattg gaggaaaaaa tcggtctata
    10201 ctatcaatta agaaatatgc gtctttactt tttttagtat actcatattt tattggctat
    10261 gttttatagc attcggaaag ttaatacact tatgtgtgat tcacccttta gggtaaggat
    10321 aaaagataaa aaatgaaaaa atattttgct gagtttttcg ggacattttg gttagtgttt
    10381 ggtggttgcg gcagtgcagt tttagcagca ggcattcctg aattaggaat cggttatgca
    10441 ggcgtttcgt tagccttcgg tttaaccgta ttaacaatgg cgtatgcagt cggtcatgtt
    10501 tcaggtggtc acttcaaacc ggcggtttca atcggtttat tagtcggcgg tcgttttaac
    10561 gctaaagatt tagtgcctta tatcattgcc caagtaatcg gtgcgattgc tgccggtgca
    10621 gtgttataca ccattgcttc aggtgttccg acttttgatg ctacagcagg ctttgccggc
    10681 aacggttatg gtgagcattc gccacacggc tatagcttaa cagcagcatt attaattgaa
    10741 gtggtattaa cggcattctt cttaatcatc attatgggtg caaccgataa acgtgctcca
    10801 gcaggtttcg ctccgattgc tattggtttg gctttaacct taatccactt aatcagcatt
    10861 ccggtaacca atacctcagt aaacccggca cgttcaaccg gtgtagcgtt attccaaggc
    10921 agctgggcga ttgagcaatt atggttgttc tgggttgctc cgattgtcgg tgcaattatc
    10981 ggtgcggtag tttatcgctt tatcgctgaa gaaaaataat ttaagcgaat aaatatgatg
    11041 cagctctagt cttgattagg gctgtttttt gtttttgaaa ggattttaaa atgaaaaaat
    11101 taatcaaagt atgtgggctg atttcagcaa ctattttcct aagtggttgt ttggtaactt
    11161 cggtggttgg tggagtggtg ggaaccgcag ttgatgtggt agacaccgta acccctgata
    11221 ttatcgacta atttactgac aagcggttaa aatttccaga tagattgcaa atataaaata
    11281 aagcgttaca atggcttgct attttataaa accgatgaat taaaggattc aatatgaaca
    11341 ttacaaaaga tttagtgcca agcattgcat atcaagtgcg tactcaagaa ggcgtattag
    11401 tagatgaagc accggtaaac caaccgttag aatatttaca cggtcacaac aacttggtag
    11461 aaggcttaga aaaagccctt gaaggcaaag ctgtaggcga tgtgtttgaa gtgcgtgtga
    11521 aaccggaaga aggttacggc gagtacaacg ataatatggt tcaacgtgta ccgaaagatg
    11581 tatttatggg cgtcgatgaa ttagaagtgg gtatgcgttt tattgcggac acggatttag
    11641 gtccgctacc ggtggtaatt accgaagtag atggcgatga agttgtggtg gacggtaacc
    11701 atatgttagc cggtcaggaa ttacttttct ctgtggaagt ggttggcatt cgtgaagcga
    11761 cagcggaaga aatcgcacac ggacacgttc acggcggaca cgaacacggt tgcggttgtg
    11821 gtgggcacga tcatcatcac gatcacgatg gttgctgcgg cggtcacgac cacgatcatc
    11881 accatcatca cggacacggt ggctgtggtt gcggtggtca tcactaactt tgtgatttcc
    11941 cctctccccg cttgcgggag agggtggagc aaatcgaaga tttgcggagg gagaggggga
    12001 tatccaaggt ttcccctctc tctgcttaaa atttctgaac gaaattttaa gctgtctctc
    12061 tcccgcaaac gggcgagagg aaatattccc tgttaaagcc attgctaaat aaccaaacaa
    12121 gcggtcaatt ttccttggaa atttaccaat gtctttatct caatctaaca ataaataacc
    12181 tttcaaaatg atgaacgaaa cacaacattc ccaatccgct atcgactatt tacgctcgat
    12241 tttaagctca aatatttatg acttagcgca agtgacccct ttacagaaaa tggaaaaact
    12301 gtctgagcgg ttaggcaact ctatttttat caaacgtgaa gaccgacagc cggttcacag
    12361 ctttaaattg cgaggtgctt ttgcgatgat ctcaaattta tccaaagcac aaaaagaggc
    12421 tggagtcatt gcggcttcag caggcaacca cgcacaaggg gtggcacttt ctgccaaaca
    12481 tttaggttta agagccttga ttgtaatgcc gcaaaacaca ccggcaatta aggttgatgc
    12541 ggtacgtggc tatggtggtg aagtgttgct ttatggggcg aattttgatg aagctaaggc
    12601 gaaagcgatt gagctttcca ccgaattggg tatgaccttc attcacccgt ttgacagccc
    12661 ggcagtgatt gcagggcaag gcacaatcgg cttagaactg gtgcaacaaa agccggattt
    12721 agattatgta tttattccag tgggcggtgg cggcttaatt gccggcattg cggttttaat
    12781 taagcaggtg atgccgaata ttaaagtgat tggggtcgaa tctaaagatt ctgcttgctt
    12841 atatcacgcc ttaaagcaag ggcaaccgac cgatttagaa cgggttgggc tttttgctga
    12901 cggtattgcg gtaaaacgca ttggcgatga aactttccgc ctgtgtcagc aatatgttga
    12961 tgatgtggtg ttggtggata atgatgaaat ctgtgcctca atgaagttga tttttgaaaa
    13021 tgtgcgtgcg atttccgagc cgtccggggc aacttccctt gccggtttga aaaaatatgt
    13081 gaaagagcat caattacaag ataaaaactt agcttgcatt ctttccgggg cgaatgtgaa
    13141 tttccacacc ttacgttttg tgtcagagcg ttgtgaaatc ggggagaaac gtgaagcttt
    13201 attggcagtc accattgctg aacagaaagg tagtttctta aaattctgcg agattttagg
    13261 caaccgtgcg gtaacggaat ttaactatcg ccattccgat gataaacaag cctgtatttt
    13321 tgtgggggtg cgaatttcag gctcggcaga aaaagtagag attattaaag atctgcaaca
    13381 aaacggctat gatgtggcag atttatcgga cgatgatatt gccaaaaccc atattcgtta
    13441 tatggtaggc ggtagagcaa gctccattca atatgagcaa ctctatagct ttgaatttcc
    13501 agagcagaaa ggggcattgc taaaattctt acaaatgctg actaactggg atatctcgtt
    13561 attccattac cgagctcacg gagccgatta tggcgatatt cttgccgcat ttgcattaaa
    13621 cagaggcgat gaagtagaat taaaccgaca tttagaacag ctaggttacc gcttccaaaa
    13681 tgtgagcgaa agtccggcat atcaatattt tttaaagtaa atttttatca atcttaggaa
    13741 ttagaatggc tcgtaaatat ttcggcacag acggtgtgcg aggtgaagta ggtaaatttc
    13801 caattacacc tgaatttgca ttaaaactcg gttgggcagc aggtaaagtg ttggcaaccc
    13861 aaggcacgaa aaaagtatta atcggtaaag atacccgtat ttccggttat atgttggaat
    13921 cggctctgga agccggttta accgcagccg gtttatcagc agtattcgtt ggtccgatgc
    13981 cgactccggc aattgcttat ttaacccgta ctttccgtgc ggaagccggc attgtgattt
    14041 ctgcctctca caatccttat tacgataacg gcattaaatt cttctcgtcg gttggtgaaa
    14101 aactacctga tgaaattgaa gaagcgattg aagcaatgct tgataagcct atggattgcg
    14161 tagattcagc aaatttaggt cgtgccagtc gtattaatga tgccgcagga cgttatatcg
    14221 agttttgtaa aggcactttc ccgtctgaac taagccttga aggctataaa attgtggtgg
    14281 attgtgccaa cggtgcaacc taccatatcg cgccgaatgt aatgcgtgag ctaggggcag
    14341 aagtgcttga aatcggtact aagccggacg gtttaaacat caacgaaaaa tgtggtgcaa
    14401 cggatattaa agcacttcaa aaagtggtgg tagaagcagg tgcggatgtt ggtctagctt
    14461 acgatggtga tggcgaccgt ttgattatgg tcgatcactt aggcaataaa gttgatggcg
    14521 accaaattct atttatttta gcccgagaag ctctacgttc aggcaaatta caaggcggtg
    14581 tagtcggtac attaatgagt aatatgagct tggaattagc tttaaaagag ctagccattc
    14641 cattctctcg tgctaacgtg ggtgaccgct atgtactaga gcagcttaaa gaaaaaggct
    14701 ggaaactggg tggcgaaaac tccgggcata ttatcgtgtt agataaaaac accaccggtg
    14761 atggtattat tgcctcactg caagtattag cagcaatggc aagccacaaa ttaagcctga
    14821 atgatttggc aaaagcagtg cctctgttcc cgcaagttct gctaaatgtt cgctttgaga
    14881 aaggggcggc aaatccgctt gaaagtaacg aagtgaaagc ggtagcggca gatgtggaaa
    14941 aacgcttggc aggcaaaggg cgtattttat tgcgtaaatc aggcactgag ccattaattc
    15001 gagtgatggt ggagtgcgaa gatgctgtac ttgctcaaag ctgtgcggaa gagattgttg
    15061 aagcagtaaa acgtaactaa tgacaagcgg tcgtttttgg taaaaaattt gccaaatatg
    15121 accgcttgtt acatatatgt gtagaataga tgtagtaggg cgaaaatttt tttcgccttg
    15181 ccgaagtggg caaatgatga tttgccctac aagtggaggt aaaaatggat caacaagaaa
    15241 tgaaaaaaat tgcggcacaa gctgcattaa aatttgtaaa atcggacact attgttgggg
    15301 tgggaagtgg ttcaaccgta aactgtttta ttgatgccct tgcttcaatg aaagatgaga
    15361 ttaaaggagc ggttgctgcc tctaaagcct cggaacagcg tttgcgtgat atcggcattg
    15421 aagtattcag tgccaatgaa gtgacggaat tagatgtgta tattgacgga gctgatgaaa
    15481 tcacgccaca aggggcgatg attaaaggtg gcggggcagc acttacccgt gagaaaatcg
    15541 tgagttcatt agcgaaaaaa tttatctgca ttgtggataa atccaaacaa gttgatgtgc
    15601 tgggttcaac tttccctctg ccggtggagg tgatcccaat ggctcgttct tatgtggctc
    15661 gtcagctcgt agccttaggt ggttcgccgg aataccgtga gggcgtggtc actgataacg
    15721 gcaatgtgat tttagatgta cataacttta aaattatgga gccgttgaaa atggaacata
    15781 ccattaataa tattgcaggc gtggtgacta acggtatttt tgcacaacgt tttgccaatg
    15841 taaccattgt cggcacacct gaaggtgcag ttattttaga ataatttatt attgcaaata
    15901 caaatactta ggagcaaaaa atggctaaaa tttctttgga taaatccaag atcaaattcc
    15961 ttctgcttga gggcgtacat caaaacgcaa ttgatgtact cttagcggct ggttatacca
    16021 atattgaagt gcataaaaaa gcattagacg gtcaagaatt aattgatgca attaaagatg
    16081 cacactttgt tggtattcgc tcgcgtactt ttttaaccga agaagtactg gctcaagctc
    16141 cgaaattaat tgcgattggt tgtttctgta tcggtacaaa ccaagttgat ttgaatgcgg
    16201 cgaaaagacg aggtattccg gtattcaatg caccgttctc aaatacccgc tcggtggcag
    16261 aattagtgct ggcagagatg attttattaa tgcgtaaagt cccgactgca aatgcggaag
    16321 tgcatcgtgg ggtgtggaat aaatcggcag caggtgcaaa cgaagtacgt ggcaagaaat
    16381 taggtattgt gggatatggt catatcggtt cacagcttag tattcttgcc gaagccattg
    16441 gtatgcaggt ttatttctat gatgttgaaa acaaactacc actcggtaat gcacagcaag
    16501 tgccgacatt agagcatttg ctttcaacct gcgatatgat ttcactacac gttccggaaa
    16561 tcgactcgac taaaaaccta atgagtgcag aacgcattgc tcaattaaaa gaaggctcgg
    16621 tgttaattaa tgccgctcgt ggtacagtgg tcgatttaga cgcattagcc gctcggattg
    16681 aagacggctc attgcgtggt gcggcgattg acgtatttcc ggaagaaccg gcatcaatta
    16741 atgacccgtt cgtgtcacca ttgtgcaagt ttgataacgt gattttaacg ccacatattg
    16801 gtggttcaac ctctgaagct caggcaaata tcggcaccga agttgccaat aaatttgtga
    16861 aatattcaga taacggttca accgtgacgg cagtgaattt ccctgaagta tccttaccgc
    16921 ttcatactaa taccaaacgt ttattacata ttcacgagaa tcgcccaggt atgttaaatc
    16981 aaattaacca gatttttgtg gaatcaaatg tgaatattgc cgctcaatat ttacaaaccg
    17041 atgcagctat cggttatgtt gtaattgatg tagagagcga caatgttgat gaagcattac
    17101 aacgtttaaa agcgatcgaa ggcacgattc gagcccgagt attatattaa aaggcaaaga
    17161 gcctgaatca cttcaggctc ttttgtgtta caagcggtta ttttttcaga aaactttacc
    17221 gctaagatta gaaacgataa gttgcattcg cggtgaaagc gttacctttt aattttgtgc
    17281 cgctacgttt taaattatcg cgagtgtatt ctgcacctaa ttcaacatcg cttgaaactg
    17341 catattttgc accagcaccg aaaccaaagc cacgataggt ttcattaccg attttgcttg
    17401 agctggcatc tacttttaca taaggtaata aatcggatcc aacacgataa ccttgttgat
    17461 aagctatagt atatttattt ttttgtttta catcgctaaa tacttttgta ctacctactt
    17521 ttgcttttcc ttgtacaacg cctacaaagt tattgccttg ctcaatacca taatcgacaa
    17581 ctaatgccgg acctgtggaa tccttgcctt ttactccttc aactttgtat tttgttgtag
    17641 ttacatctac accgacaccg atgccagtaa atgtatttcc gacaggagcg gctaatagat
    17701 gggtagaaaa taagcaagtt aatgcgacaa tagttaattt tttcataaaa ttactcctat
    17761 cttgattaag gttcgatgtt accacagaaa cggtagcctt caccgtgaat ggtgacaata
    17821 atttctgggg tattggggtg gtcttcaaaa tgtttacgaa tacggcgaat agtaacatca
    17881 acggttctat catgaggttt tagttcccgc ccggtcattt ttttcaataa atcttcacgt
    17941 gtttgaattt ttccaggatg ttggcaaaaa tgtaataaag ccctaaattc actacgtggt
    18001 aatttattta cctcaccttc tggtgagatt aaactatgac tgttaatgtc taagatccaa
    18061 ccgttaaagc gatagctttc aatttcagat tgctctttta caacgtcgtt ttctttatct
    18121 atcatcgttc gttgcaataa atttctcgct cggatagtca attctcgagg attaaacggt
    18181 ttagtgatat agtcatcggc acctatttct aaaccgagga tcttatcaat ttcattgtcg
    18241 cgaccggtta aaaacattaa tggtagcttc atattctctc gaatttctcg ggcgagaatt
    18301 aatccatttt ttcccggtaa gttaatgtcc attagcacaa gattgatttc atttgtactc
    18361 agaacagagt gcatttcctc gccgttagat gcttgaaata cctgatatcc ttctccttca
    18421 aaaatgcttt tcagcatatt ccttgttact aactcatcct caacaattaa gatttgtggg
    18481 atcttcatct tttaaacctc tgatttaatt tataaattct gtttaataac gtttatgtta
    18541 caaatttctc gcattttact tataaattta tgaatgggaa gggtaaaatg acaatttgtt
    18601 taattcagtc aaatttttgg ctatttctct cgatcatcaa cacctaaaat tagataattt
    18661 ccattaatat ccgctaggct acgaaagccg atattataaa tagcacattc tctgctgaca
    18721 tttagattgc cataattaaa attatcatgg tagtggccat ggaaaatttt gctcgcccct
    18781 aattttcttg ctagctggtt gattacagag aagccttgag ggtgtggctt tggtgcttcg
    18841 tgtgtaatta aaatatcggc tttttcattt tccaatgctt caaaatcaga agggaagatc
    18901 gaagagcggt gccttaatgg aataccacca cgccaaattt tttccggagc agagtattgg
    18961 caaaaatgga tgggatcaaa aaacattgga cgattgggcg gcatccaaat atgacctcgg
    19021 aagacccctc caatacctgc aatttttatt ccttgaatgt caactacttt accgtgaata
    19081 ttgcgttctt tccagctact attccaaata gaatcaaagg cagcaacggt tttactgtca
    19141 tggttaccat gaataaacca aatatcacaa tactgcgaaa gccgatctaa ttcatcagga
    19201 gtagtcaatt gaagatcgcc tagaataact aaagctacct cctccgtctg ctgttgtaga
    19261 atcggataaa gatgctcgta gctaccgtgt ggatcgcctg caaaaagtat catgttattt
    19321 cctgtggcag ttattcaaat tcgtgaatat tatattgctt aaactgtaat gggattggtt
    19381 cttcatttaa aatagcttct accacctctt tggcttgatt gtagcgatcc aaataacttg
    19441 gagattcaat tttaatataa ggaatatggt gtttttctag tagttttttc agtaggtgtt
    19501 gaaactgttg acgctgtttt ttcgatccta aactgcgtaa accatcggct acccattttg
    19561 tattgttaga aagtaaaatc gtcacatcaa aaggatattc gcggatcatt gaatccagaa
    19621 acgggtgagc cttcccttca tactgaatac agaacgcttg agttgtgatg taatcggtat
    19681 caataatagc cactttatga gcatgtttca tggcataatc aacgtagcgt tgatgtccaa
    19741 gtaccatttg cggatagtcg gaatattgca ttgcttgttc attgccgcca agttgctcaa
    19801 acacaaactc gcgcccatat tcccaagcag aggtagtgtt aaatacattc gctaatttgc
    19861 tcaccaacac tgatttacca ctgctttcac cacctaaaat cgcaatagtt ttaacaaaga
    19921 aaggacgaac ttctttcgga ataaagcgcc agtattgaaa aggattatta cgaatttgtg
    19981 ttgctgaaat attaaaggat tcacgagtag gatcgacaag ttgaacttct agatgcaaat
    20041 atttttcata aggttcttta tcttgaattt cactactaaa aacaatagta ggattaatgc
    20101 ttttttcttc aaatagcgct ttaacacgac cagaccaatc ttcccagcca tttggatagc
    20161 tcggaatacc gtcttcaact aaatggtgaa taaagatctg tttttgttgg tatttaaaaa
    20221 tttgctgtac ccagcgtagc cgatcttcat ttgtcggcat tcgtttcatt ttactttcct
    20281 ggaaaagacg taaatcccgt tcagaatcgg tacaaacaat aacatgtaaa acatctactt
    20341 tactaaatgc ttcatagatc atattaatat gacctgtgtg aataggatag aatttgccaa
    20401 aaataacccc ggaacgctgg ttgctatctt ctacgatatc taaaacggaa tgcaaggctt
    20461 taagcttatt ggcgcttgga tttttgattt ttccgcttac taactgatta aaataagctc
    20521 gggttacccc agcttgttca caaatatcat tcactttgag tttaagctgt ttgcgctttt
    20581 gttgaagata agcaaaatct gtcataatga ttccctcgat tttttgatga ttatcatata
    20641 gtaaaatgta gcatttgaat atatttttat tgtataaata gcagttttat agcatatctt
    20701 tgctttaata attagctaca cgaccacttt cagcactaaa aaaatataac agattgaatt
    20761 tttttccttt tttgtagatt tctcgttata atcacagcaa ttttcactta taaaatggtt
    20821 tatcttttat gcaacttatt cgaggtttcc acaatttaga caaacacatc gctttaaatc
    20881 aaggctgtgt tctctctatc gggaatttcg atggtgtgca tcttgggcat caaaatattt
    20941 tggctcgtct ttgtgataga gctttagatt taggattgcc ctcagttgtg atgttatttg
    21001 agccacaacc tcgagagttt tttgcaaaaa aaagtggaaa ttcaactgct tgtaacccgc
    21061 ccgcccgctt aatgcgcctg cgagataagc taaaatattt ggaagaagcc ggtgtggatt
    21121 ttgtgctatg cgtgcgtttt agtgaaaaat ttgctcaaat ttcggcagac gatttcatca
    21181 gcgatttgct cgctaaaaga ctgaaagtac gctatttaag tgtaggcgat gattttcgct
    21241 ttggggcaaa acgggcgggc agttttgaaa ctttacgcaa tgccggctta aaatatcgct
    21301 ttgcggtgga agaaagccat acccatagct taaacgaaga aagaattagc agctcattag
    21361 tgcgagaagc cttacaaaac aatgagttag atttggcaga gaaattactc ggcaagcctt
    21421 attcgattct gggacgcgtt gcacacggta ataaactcgg cagaaccatt ggttttccta
    21481 ccgcaaatat tatgctcaat cgtttggtta cgccgattca agaggtattt gtggtaaaag
    21541 tgaagaccaa acagggcagt tttaacggaa tcgccaatgt cggcaaccgc ccgaccatta
    21601 acggtactaa gccgttatta gaagtgcata tttttgattt caatgcctct atttacggtg
    21661 aggcgattga agtgatcttt ttaaaaaaat tacgcaatga aataaaattt cccgactttg
    21721 aagcactaaa agcccagatt gagaaagatc gcttgcaagc ggttgaattt tttaaggaat
    21781 ttgcaaggtg agcctttcta gtttccagcc ccagaggggc tgagctatgc ttaacctagt
    21841 acggctatgc cgtgctaggc ataaaacaga taattttaac gagaaataaa aaaatgacta
    21901 ttgactacaa aaacacctta aacttgcctg aaacaggctt tcctatgcgt ggggatctcg
    21961 cgaaacgtga acccgatatg ttaaaaaatt ggtatgacaa aaatttatac caaaaaattc
    22021 gtactgcctc gaaaggcaag aaaaccttta ttttgcatga tggtcctccg tatgctaacg
    22081 gtaatattca cttaggtcac gcagtgaata aaattttaaa agatatgatt ttgaaatcga
    22141 aaaccgcatt aggtttcgat accccttatg tgccgggctg ggactgtcac ggtttgccga
    22201 ttgagctgaa agtagaaggg attgttggta aaccaaatga gaaaatttcg gctgccgagt
    22261 ttcgccaagc gtgccgtgac tatgcgaaag aacaagtaga aggtcagaaa gccgatttta
    22321 tccgtatggg tattttaggt gattgggata acccatacct aacaatgaat tttaacactg
    22381 aggcgaatat tattcgcacg ctcggcaaag tgattgcgaa cgggcattta tacaaaggtt
    22441 caaaaccggt acattggtgc ttagattgtg gatcttcgtt agcggaagct gaagtggaat
    22501 atgaagataa agtttctccc tctatttatg tgcgttttaa tgctgtggac gtaaatgcgg
    22561 tagaagaaaa attcaacgca cagggtaaag gtagaggggc attatctgcg gttatttgga
    22621 caactacccc ttggactatt ccgtctaacc gtgcgattgc gatcaaccct gaattagact
    22681 atgccttagt ccaattaggt gatgagcgtg tgttactagc ggtcgattta gtcgaagatg
    22741 ttgcaaaagc agcaggcgtt gaatctgctg agattttagc aaccaccaaa ggcgaaaact
    22801 tagaattatt acgctttaac cacccattct atgattacag cgtgccattt atttttggcg
    22861 accacgttac cacagacggt ggtacagggt tagtgcatac tgcaccggat cacggtgcgg
    22921 acgacttcgt ggtcgctcgc aaatataaca ttgaaatggc agggctaatt tccaatgacg
    22981 gtaaattcaa agcagacacc ccgttctttg ccggcttagg tgtgtttgaa tccaacgata
    23041 aagtagtcga aaaattacaa gaagtgggag cattattaaa attatcccgc atcaagcata
    23101 gttatccgca ctgttggaga cacaaaaccc cgattatctt ccgtgctacc ccacaatggt
    23161 ttatcggaat ggaaacccaa ggcttacgcc agcaagcact aggagaaatc aaatcggtgc
    23221 gttggatccc aagctggggt gaagctcgta ttgatacgat ggtggcaaac cgcccagact
    23281 ggtgtatttc acgccaacgt acttggggcg tgccaatggc gatgtttgtg cataatgaaa
    23341 ccgaagaatt acatcctcgc actttagaat taattgaaga agtggcgaaa cgtgtagaag
    23401 aaaaaggcat tcaagcgtgg tgggatttag atcctgcgga attgctcggt gaagaagcca
    23461 aagattaccg caaagtgccg gataccttag acgtgtggtt tgattcagga tcgacctatg
    23521 cctctgttgt agaacaacgc cctgaattta acggcaattt agcggatatg tatttagaag
    23581 gttcagacca acatcgtggt tggtttatgt catctttaat gctttccact gctactaacg
    23641 gcaaagcacc atacaaacaa gtgctaactc acggttttac cgtagatgag aaaggcagaa
    23701 aaatgtccaa atcgcttggc aacgtaattg tgccaagtga agtgtggaac aaaaacggtg
    23761 cagatatttt acgcttatgg gttgcctcca ccgactatac cggtgagatt accgtttcgc
    23821 acaatatttt aaacagtgcc ggggaatcat accgccgtat tcgtaacaca gcccgtttct
    23881 tattggcgaa cttgaacggt ttcgatccaa aaagtgattt agttcaaccg caagagatga
    23941 tcgccttaga ccgctgggcg gtggcttgtg ctttagaggc tcaaaacgac atcaaagaag
    24001 cctatgataa ctaccaattc cacacggtag tacagcgttt aatgcgtttc tgctcgattg
    24061 aaatggggtc tttctactta gatattatca aagaccgcca atacaccacc aaagccgaca
    24121 gccttgcccg ccgtagctgc caaacagcgt tatggcatat tgcagaagcg ttagtacgtt
    24181 ggattgcacc gattttatcc ttcacagccg atgaaatttg gggctactta ccgcaggtgg
    24241 aaggcagaag tgaatttgtc tttaccgaag aattctacac cggattattc ggcttaaccg
    24301 agcaagataa attagatgat gcttactggc aacaaatctt aaaagtgcgt gcggaagtca
    24361 accgtgtgct ggaacaagcc cgtaaagaca aagtaattgg tgctggctta gaagccaagg
    24421 tgacggtatt cgccaatgat gaaattcgcc cgttattaga acaacttggc aatgaattac
    24481 gctttgtgct gattacctca caagcgattg ttaaaccact ggcggaagca gatattgcgg
    24541 aaggcgaatt agccggactc gcagtaaaag tggaaaatgc agatggtgag aaatgccctc
    24601 gttgctggca ttatgctacc gacatcggct cgcactctgg ccacgaagaa gtttgcggac
    24661 gttgtgtgga aaacgtagcc ggcgaaggcg aaaaacgctt gtttgcttaa aatgtaattc
    24721 aattcatctt tctaaggcag aggaaactct gccttagctt ttccagcgat agatagacac
    24781 tcagtttaaa atttgcaaaa attttcggaa aaataaccgc ttgtattgat gtaaatgatg
    24841 aaaataatcg cttttctttt tatttttctc tccgtttttt ctcacactgc tttggctaat
    24901 tctatggctg aacaatgtgt cgcgttaaaa tctaactcgc cggaaaaaac ggctatttat
    24961 aaagctgaat gggttgagaa atctgctgag ttgccggctt actgtttagt acaaggtgaa
    25021 attggaaaac gggtgggggt ggataatgtt ccttacggta ttcaatttga gctacgttta
    25081 cccgaacaat ggaatgaaaa attcttattt cagggggcgg gcggtgtagg tggggtgatt
    25141 ttccctgcca ttggtaagct ctatccgcac ggggcgagtg gtgcaaatgg cttaaacaga
    25201 ggctatgcag tagtgagtaa cgacagtggt cacgccactc gagatttagt gtttaccgca
    25261 gatcccgaag ctcgctcaaa ttatgcctat gcctctatcg gcaaagtgac cacggttgcc
    25321 aagcagttga ttgccgaatt ttaccgaaaa ccgagcaagc acaattacat tatgggctgc
    25381 tcgaatggtg gtcgtgaggc gatgatggcg gcaatgcgtt atccacagga atttgacggt
    25441 gtgattgcag gcagccccgg ctttcgggtt tcccgttcag tcttggcgga agtatgggat
    25501 aatcgagcct tgttggcagt tgctccgaag aatggtgatg gcgacaaaat tttatcccaa
    25561 gccctcactc aacaagattt agatgtgatt gccaatggtg tattaacacg ttgcgataag
    25621 ttagacggct tagcagacgg tttaattaat gcgtgggaac agtgtgattt ccagccggaa
    25681 atggtggcaa agcaactggg gcagaaaaaa gtcgctttaa tcaaaacgat tttcgagggg
    25741 gcgaaaaaca gtcgaggcga gctgatttat agcccttggg cgtatgattc cggcattaac
    25801 gctaaaggct ggcgggattg gaaattaggt gatagccaaa ccgctcagcc caacagcatt
    25861 agttttaaaa tgggattgaa aagtttgacc cattactatt tagcccccag agtgccgagc
    25921 cgagatccac tcagcgtaga tttggatcaa gccagcgaac aagtgaaagc ggtagggagc
    25981 attcacgatg cagatcaagt tgatttatcc gcttttaagc agaacggtgg caaaatgctg
    26041 atttatcaag gcgtttcaga cccgattttt tcggcagttg atctcaaaaa ttggtatcaa
    26101 agcctacaac aagcggtcga aaatccgcaa cattttgcaa aattgttttt cgtgtcggca
    26161 atgaaccatt gtgggcgagg ggcaacggtg aatgattttg atatgctcac tgcccttgaa
    26221 aattgggttg agaaaggact ggagccggaa agtattgccg ccaaagccgg tgagctttac
    26281 cctaataaag ccaagcagat cccgctttgt gcttatccga aagtcgcaat ttatcaaacc
    26341 ggagaagatc ctaatcgttt gaccagctat caatgccgtt aagcgaaaat cgctttattg
    26401 gcgatttcag tgtaaaatag caggcaacga ttgaaaattt tggaatcaat gatgttaatt
    26461 ttgaacaaac aacgtaaagc tgaataccta ttgaaaagcc tgccatactt accacaagtg
    26521 gcagagcagg ttcagtttct cgcctcgagc aaagcgttta aacaacaaat tttacagctg
    26581 attcaaacgg caaaaaagcg tatttatata accgctcttt actttgaaaa agatgaagcc
    26641 gggcaggaag tattagctgc tttatatcaa gcgaagcgaa ataatcctga actggaaatt
    26701 aacattttgg ttgattggca ccgagcccaa cgtgggcgta ttggtgaggc agagaattta
    26761 tctaacgccg actggtatgc aaaagttaaa tacgaacaag gtttaccggc agggcaagag
    26821 attgcctttt acggtgtgtc gattaacgga cgagaagtgt ttggtgtatt gcatttgaaa
    26881 ggcttcgtat ttgacgatac gattttgtat agcggagcaa gcattaataa tgtgtatttg
    26941 catcaatttg aacgttaccg ttatgaccgt taccacgtca ttgaaaataa agttcttgcc
    27001 gatagctggg tgacgtttat tcaacagcat cttttaacca atgaagcggt aaaacgttta
    27061 gatacggaaa atcgcccgaa aacggctgaa attcgtgcgc aaattaagca tttccgcaaa
    27121 caattaagtc gggaagcgta tttatttaac ggtgttgctg aaaatggtcg cttagcggtt
    27181 tctcctttag taggcttagg gcgacgtaag aatcagttaa ataaagtgat tgaagcgttg
    27241 ttttatcaaa ctgagcagaa attaacgatt tgcactccgt attttaattt tccgcattct
    27301 ctgagaaccc gtattgaatg gctattggca aacggcaaat cggttgaaat tattgtgggg
    27361 gataaaaccg ctaatgattt ctacaccaag ccggacgaaa aattcacgat ggcatcggct
    27421 ctgccttatt tgtatgaaaa gaacttacga gcctttgcaa aacggttgga cggttatgta
    27481 caaaataaac agttaaccat tcgcttatgg aaagatggcg aaaacagcta ccacttaaaa
    27541 ggggtttggg tggataaccg ctatattttg ctgaccggca ataaccttaa cccaagagcg
    27601 tggcggttag atgccgaaaa tgcaatttta atttccgatc ctcaacatca attaagtgaa
    27661 aaagcagaaa cggagttaaa ccaaatttgc caacatacgc agattttaac tcactacagt
    27721 gatttggaag ttttaaccga ttaccctgaa aatgtgcgta aattgctgaa aaaattcggc
    27781 agggttaagt tggataaaat tgtgaagatg ttgctttagt taggtattta tatgggcgag
    27841 ctgatgggtt cgcccaatta ttaaggataa aatatgagcg aacaaattta tggcattcac
    27901 gcagtgaaag ccttttggga taatgcacct gagcgtttaa ttgaagtgct ggtgctaaaa
    27961 gggcgtgaag ataaacgctt aatgccacta ttaaatgaat tacaacgttt aggggtggca
    28021 attcaacaag tgaaccgcca aaccttagat aacaaatcgc aaggtgaagt gcatcaaggg
    28081 attattgcca gagtcgtgcc acaaaaagag ctgaatgaac acgatttaga ggcgatctta
    28141 agccaaaaga aaaatccact cttgttgatt ttagatggcg tgaccgaccc tcacaaccta
    28201 ggggcttgtt tgcgtaccgc tgatgcggca ggggttgatg cggttattgt gccgaaagat
    28261 aaatctgctc agcttacacc aaccgctcgc aaagtcgcct gtggggcagc ggaagtgatg
    28321 ccactgattc gagttaccaa tctggctcgc acaatgcgag atttacaaga acggcataat
    28381 gtctggattg tcggcacggc aggtgaggcg gaatcgggca tttatgaagc aaaattaacc
    28441 ggctcgattg cgttagttat gggggcagaa ggtgacggaa tgcgtcgcct tacccgtgag
    28501 cattgcgacc agcttatcag cattccaatg gcgggctcag tatcttcttt gaatgtctca
    28561 gttgcaaccg gggtctgtct gtttgaaatt gtacggcaga agctggctgg gcaataagga
    28621 ataagtaaga aaaatgcgaa gaactgatgt cttcgcattt ttgtttttag agccgcaaaa
    28681 aatctcagaa attgaaccgc ttgttagccc acatactctt ccaaattaat cactttatcg
    28741 cacgatttct caattagata aggatcgtga ctgaccaaaa tcagactgca atttctcgct
    28801 cggcactggt tgatcagcaa ttctatcgtt tcttgttgtg tgattgggtc aagccgagaa
    28861 gtgacttcat cggcaaacag tagtaccggt tctaagagca aggctcgcaa aattgctact
    28921 cgttgtagct cgccgccgga gacattttcc gcattgcgtt gcaaaatttc tgggttgagc
    28981 gaaagttgtt gcagtaaact tggaatcggc agtcgatcta attggtgctt gttaatcaca
    29041 tcatcaagca aggtttttag actgacattc ggagcaaagg cttccggtgg gtcttggtaa
    29101 agcttcaaca cttggtgttt tttatggctg tggctgtgcc acacaacttc gcccgatttc
    29161 ggcttaagca aaccacaaag tacatcggca agcgagcttt tgccaatccc actatgcccg
    29221 acaatcccaa gcacttcgcc tttgtgtaag tcaaaggaaa ggttgctaaa cagcgttttc
    29281 ttaccacggg caatactcaa atttttaacc gaaacaagcg gttgtttttg gcttaaattt
    29341 tgcagatctt gcaatggttg ccaatgtttt ggatcggcac tgataagtgc tttggtgtaa
    29401 gggtgttgtg ggttatttaa caacgctttt gcctcacctt gctccaacag ttcgcctttt
    29461 ttcatcacta aaatagtgcc accgagttgc tcggcaactt caatatcgtg agtaatggtg
    29521 agcaagcctc cgccgttttg gtaggcggat tttaacagtt caatcacttt gcctttactg
    29581 gttgggtcaa gccctttggt cggctcatcg gcaagtaaaa ttttgccacc ggcggcagtt
    29641 gcaatcgcaa aagaggcacg ttgagccatt ccaccggaaa gctggtgtgg gtagttttca
    29701 accgattcgc ccagcccaag tttggctaaa taggctttgc tttccgtttt ggcttgagat
    29761 gatggtttgc ctgccacata gtgaaaactt tcccacaact gcttgccgat tgccataatc
    29821 ggattaagcg agcggcgagg ctcttgtggc agcattacca aggttttacc ccacaatgtt
    29881 tgcaagcggt cggattcggt ggaaattttg caatttttta ccgaaatttc accgcttgcg
    29941 attaagccgt cgggtaacgc ccccataatc gcttgaatca gcaggctttt gcctgagccg
    30001 gtttcgccta agatagtaat attttttcct gcctttaatt gtaggctaat cggttcgacc
    30061 agctgtaatc tgttttcggt ttcaacagat aggttttgga tattgagtaa tttcatcttc
    30121 attttattac tgtaggggcg ggccctgtgc ccgcccataa atataataag aattttgggc
    30181 aggcacagga cctgccccta cgcattattt aatttttcta gacaataatt gtaagctgag
    30241 taccattaag aaaacggcaa ccactggttg caaaaatatc agcggtgctt cgtagtagta
    30301 agggaatagt tcggtcatca tcaagcctaa ttctgctgta ggcgggcgaa gccctacgct
    30361 gacaaagcca agtgtggcga gagctaaaat cgcattgccg agcgagaatg ccgataaagt
    30421 ggcaatcatc ggtaataaac ggggcaataa gtggcgtttg aagctataaa accagcccat
    30481 tcccattagg cgtgaggctt gaatttccga gctgtttgcc attgttgagc tgatagagcg
    30541 gatcacacgg aaaaattcca cccacattac caatgaaatc cctaaataga gcgtccaaaa
    30601 tgagccggga gaaagggcgg caaagagcaa tactaaaagt aagccgggga gtgccatcac
    30661 tagatcacaa ataaagctga aaatgcggtc aatccagcca ccgaaaaagc ctgccataat
    30721 gccggtaatt aagcctgcaa ttaaggcaca aatcacgcta aacagtgata agccaaacga
    30781 aagacgaata gcagaggcaa gacgggcaag catatcccgc ccgagatgat cggtgccgaa
    30841 ccaagcgatt tcatttggat tggaaaggat attggttaaa tcttgaaagc caatatccat
    30901 cgggtaaaaa taaggctgta aaaaggcaaa tcccaacatt gcaaggagaa tagtgccgcc
    30961 taagtattgt gaaattgaaa ttttcatctt gatgtcctat cgcttaattc gtgggtcaat
    31021 ccaagcactt aatagatcgg caatggtgtt taatacaaca aacaatccgc ccatcacgag
    31081 cgttgtacct tgaatcatcg gcacatcacg ggcaatgatg gcgtgaacca gtgcgtgtcc
    31141 gctgccgggc caagcaaata ggctttcgac caccacaacg ccttcaatta aataaactaa
    31201 ttgtactgcg tggtaggcaa tgaccggcac ggcaatattc ggcaaaccgt gacgtaaaaa
    31261 ggtatgccaa cggcttaagc cttttaattg tgaaaattgg taaaattctg agccttttac
    31321 ctgaaccatt gcagaacggg tgactcgtac cgaaaccgta ctcaagccaa tcgcaagcgt
    31381 taaggcaggc aagataaaat gttgccaact gccgtatccg ccggcaggta gccattttag
    31441 ttgagcggaa aacagcgtaa tcaagccaat cgcaataata aatgccggca cggagcgaaa
    31501 cagggtactg aataccagtg tcaagcggtc aaaaaagccg tttggcttac gggcggctaa
    31561 caaaccgagc ggaggcccga taaggattgc taagataagt gccacaatcg caaggcttaa
    31621 ggtatgcccg aattggtgct gaatttcaga ccagaccgga tcaccggtca ccaatgattt
    31681 gcctaaatta aattgagcca agtcacttag ccagctgaag tagctttgcc accaaggttg
    31741 gtctaatccc agctcggcac gcacagcctc agcagcagca ctatctgctt gatcataacc
    31801 atagcggctg gcagcaatgc ggtatgccat atcacccgaa agttggcggg tgaggataaa
    31861 tgtgagtgtg ccaaccgacc aaatcaccag tagaatttgg ataagacggc ggaagagaat
    31921 tttgtgcatt gtattatttc atttattaaa aagatgattt tatgcgttct gatccccctc
    31981 tttagcaaag aggggaaggg aagatttggc agaagaaata agcggagaaa ataggtgaaa
    32041 aatgagaaaa aacgataatt ctgtcactat ttattcacct aagagcgcaa attactactg
    32101 aaaaatccta ccttcccttt tctaaagcga ggagtatttt gaattaaact acttacttaa
    32161 taactccaaa aagaagcgtc tttcaaatgg atctagcgtc acatttttca cttctttatg
    32221 tgccaccaca ttttgttggt aataaaccac tggaatgatt ggcaactcat tgtaaataat
    32281 tttgctaacc gtttgcttta atgctttcgc tttttgcggg tcacgctcgg tttcgagcga
    32341 tttgactgca ttgtctacct cagtattttg ccaattcatt accccccaat cactgccacc
    32401 attggcgaag tcggaggcaa ttacgccata tggatctaag gttttgccat aatttaaggc
    32461 ataaagagcc atttctaaac tgccgtcttg atgccccgcc ggaatttcgc tgaaattacc
    32521 caccgaaaca ttcacttcca cgccaatttg tttccattga gcttgtaaga tagtagcaat
    32581 caccggtaat tcaggacgat ctgagaaggt gcgcatggta aagctaaacg gtttgccgtc
    32641 tttggttaag cgaccttgtt catcaaaggt atagccggaa gcggtcagat tttgtttaat
    32701 ttttgcaata tcgaccactt gttctgcggt atcaacacgc cagtcggcaa aggctttcgg
    32761 taaaatttga tcggcagctc cgttctcaat ttttaagacc tgctcagcaa tcgccttgcg
    32821 attaatcgct tgcgataagg cttggcgaat cgctaagtcg ttaaaaaacg gctttgccac
    32881 gtccattttc aactgaatag tacgggcaat agatccgctg actaattgca ggttaggatc
    32941 ggctttcagc cttgctacac tggcggtatc taaattaaat accagtgatg acggctcgct
    33001 ttgtgccatt aacatacggg tttcgctacg gctgctggct aaataattgg cttgctgaac
    33061 ttgcggtttt ttgccccaat agccctcaaa ttgcaccgct tccaatttct gcggagcttc
    33121 aatttttgtg ggtttaaacg cacctgtacc gataatttgg gtcacttcgc ctttttcatt
    33181 atagctttca ggtgccaaaa ttaaggcggt gtagtgtgtt aaaaaggaag gaaacggcac
    33241 aaaagcttta gttagctcaa tttccacttt gttatctacc gctcgaattt ccttgataaa
    33301 ggcttttgct aacacacccg gtttgctttt tgcgatattc aaactattta ccactgcttg
    33361 tgcggtaagt acttgatcgt tatgggattt taccccttca cgcaaggtaa aacgccagat
    33421 tgttccctct tcgttactgc gccagtcagt ggccaatgcc ggaattaaat tcccgttaag
    33481 atcagcttcg accaatgttt ccgctaagtt catacgctgg aaaattacgc ctgattgatt
    33541 cgggtcaagg ctattgattt cccatggggc aaccacggtt gcaagcggta atatttccgc
    33601 atttttttgc gattgagtat tctctacttg cggtttttct gcggttgctt gagtgctgtt
    33661 cttatcatcg caagcagaaa cacagaacgc agcgacagtt aatggaatca accatttttt
    33721 cattttattt tctccataaa aaaatcaggg ctatgaatat tggcgaactt tagcatattt
    33781 ccataaaaaa agtagagttt tctgcgaggg tatttttgag tggttaatta ataggcagaa
    33841 taatctcaag ataaggattg ttgatagggg aatatttagg ttatttgagg aacagaatgc
    33901 ctagtattgt aaaattcgga aaaaaattga ccgcttgtgc tttatttgta ggggcgaaag
    33961 atttttcgcc cctattggtt ttagtgctct ctcgttttaa agaactgtac gtcagggtaa
    34021 cgttcttggg cgaggtttaa gttcaccatt gtcggagcaa tgtaggtgag gttgtctccg
    34081 ccgtctaggg caaggtttgc ctcgtttttg cgtttgaact cttcaaattt tttggcatct
    34141 ttgccttcga cccaacgggc tgtggcaacg tttacggttt cataaattgc ctctacaccg
    34201 tattcggttt tcagccgtga aaccaccaca tcaaactgta gcaccccaac cgctcccacg
    34261 atcaaatcgt tgttgattaa cggacggaat acttgcaccg caccctcttc ggaaagttgt
    34321 accaagcctt tgagcagttg tttctgtttg agcggatcac gcaggcggat tcggcggaac
    34381 agctcagggg cgaagttcgg aataccggtg aatttgaaat tttcgccttg ggtgaaagta
    34441 tcaccaattt ggatcgtgcc gtgattgtgc aagccgataa tatcgccggc gtaggcttct
    34501 tcggcttgtg aacggtcgcc cgccataaag gttaaggcat cggagatcac cacatctttg
    34561 ccgatacgga cgtgtttaag cttcattcct ttttcatatt tacctgaaac gacacgcata
    34621 aaagcaactc gatctcggtg tttcggatcc atattcgctt gaattttaaa tacgaaaccg
    34681 ctgaattttt cctcgcttgg tgaaacttca cgttcgtccg attgacgggc ttgtggtgcc
    34741 ggtgcccatt cggttaaacc gtcaaggaaa tgattgaccc cgaagttacc cagtgcagtg
    34801 ccaaagaaaa ccggtgtcag ctcgcctttg ataaattcgg cgtgatcgaa ttcgtgcgaa
    34861 gcgccttgta ccagctctaa ttcatcacgc aactgctggg ctaaatcatc gccaacggct
    34921 tgatccagtt ccggattatt taagccttta accactcgta cttcttgaat ggtatgacct
    34981 tgtccggttt ggtataagta ggtttcgtcc ttgtaaaggt gatatacccc tttaaacaat
    35041 tttccgcaac caatcggcca agtgatcggc gcacatttga ttttcagcac gctttccact
    35101 tcatctaaca gctccatcgg gtcacgaata tcacggtcta gcttgttcat aaaggttagg
    35161 attggcgtat cacgcaagcg ggtcacttcc attaatttaa tggtgcgttc ctcaacccct
    35221 tttgccgagt cgataaccat taagcagcta tctaccgcag tcaaagtacg ataagtatct
    35281 tccgagaagt cttcgtgccc cggtgtatcg agtaagttta ccaagcaatc attgtacggg
    35341 aactgcatca cggaggtggt aatcgaaatt ccacgttgtt tttccatctc catccagtcg
    35401 gatttagcgt gttgtgccga gcctttgcct tttaccgagc cggcagtttg gattgcattt
    35461 ccgtagagca atactttttc ggtaatggtg gttttacccg catcggggtg ggaaataatc
    35521 gcaaaggtgc gacgtttatt aacttcttgt gggtatgaca tttttctcaa ccaattaaaa
    35581 taaaaagggc ggaaagtccg ccaaaaaatt aagcgttatt ttcgctgatt tgaggcgaga
    35641 gggcaagcgg ttaaatttgg cggaaatttt gcaaaatcgg ctagatttgc aaaaattttg
    35701 taaaaatcga ccgcttgctt tggcacaatt ttggaaacag ggtagaataa ccagctttga
    35761 ctttattatc gatgtattat cgatgaatct attatgatga aaaaatttat tcctgttatg
    35821 attgcggctc tggtgttaag cgcctgttcc tcccagccgg caaaaaggaa aagcacacca
    35881 cggattaaag caaccgctgc ttatattcca accgaaaatt ccgcttatgt ggtgtttgac
    35941 ttaaaccaaa agaaattttt ggaaggtaag aatttcaatt tagttcgccc gattgcttcg
    36001 gtcacaaaat taatgacagc agtcgttttc ttagaagaaa accgctatgg ggcgagttgc
    36061 caaacccaaa ttttgccgtc agatgctgat ttcttaaaag gcacgaccag cccattgccg
    36121 aaaaatctta acattgcctg tagtgaattg ttaaaagcga tgctagttcg ctccgataac
    36181 tatgccgctc acgcccttgc ccacgcaaca catttgaata aagcccaatt tttggctcga
    36241 atgaacagca aagcccgtga aatcggtatg actcaaacct actttggcga tagctcaggc
    36301 ttatcttcgg ataatgtttc aacggtggtt gatcttgcta aattggcagg ctatgcggca
    36361 agtaagcctg aaattcagca attgtctaat tcgccattag tgagtgttta tgcaggtggt
    36421 cgttatttct ctatgaaaaa caccaacagt atggttcgtt cccaatccta tcaggcatta
    36481 gtgagtaaga caggttatac ccgtgaggcg ggttataatc ttgcctttat tgctaaaggg
    36541 gattgcagag gcaagcgaat tggtgtggtt agtttaaaca atgataacgc tgcttcacgc
    36601 accgatttca ctgaatcgat gttagcgaag tatcaatgcg taccaaatcc gtatagacaa
    36661 tatgattaat ggttgccttg agaaaatagc ggtttttctc taaaatactg ccttttaaat
    36721 taaatgaatt gagacaagat tgtgtcagaa attaaattaa ttgtcgggct ggcgaacccg
    36781 ggggtaaaat atgaagaaac ccgccataat gcaggcgagt ggctgattaa cgaacttgcc
    36841 cgaatatata acgttagcct aaaagaagag ccgaaatatt ttggcaaagt agcaaaaatt
    36901 aataccgctc aaggcgaagt ccgtttatta gtgccgacta cttttatgaa tttaagtggt
    36961 aaagcagtgg gagcgttagc caatttttat cggattaaac cggaagaaat tttggtcgct
    37021 cacgatgaat tggatttacc acccggttcg gtgaaattga aaaaaggcgg cggacacggt
    37081 ggtcataacg gcttgaaaga tattattgcc gcccttggca ataataaaga gttttaccgt
    37141 gtgcgaattg gtattggaca cccggggcat aaagacttag tggcgaatta tgtgctgggc
    37201 aagccttcac caagcgatca gacattactg aatagtgcgg ttgatgaagc cgcacgttgc
    37261 gtagaagttt tatttaaaga cggaatggtt aaggcaacta accgtttaaa tagttttaaa
    37321 gcaaattaaa actctctccc ttg
//
LOCUS       DANKBV010000007        35482 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.007, whole genome shotgun sequence.
ACCESSION   DANKBV010000007 DANKBV010000000
VERSION     DANKBV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 35482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35482
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.007"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            15..488
                     /gene="folK"
                     /locus_tag="PWY43_000264"
     CDS             15..488
                     /gene="folK"
                     /locus_tag="PWY43_000264"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDL2135182.1"
                     /translation="MQTVYIALGSNLENPLEQLKQAVEKLKKLANEFEISPFYGSKPV
                     GPQDQPDYVNAVAKFSTDLSPTALLDKLQSIENEQGRVRVRRWGERTLDLDIILYGNQ
                     QIQTERLNVPHIEMKNREFVIVPMFDLTPDLVLPTGEKLADIYAQFKDHQMIIFS"
     gene            complement(480..1635)
                     /locus_tag="PWY43_000265"
                     /pseudo
     CDS             complement(480..1635)
                     /locus_tag="PWY43_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484106.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2300..2569)
                     /locus_tag="PWY43_000266"
     CDS             complement(2300..2569)
                     /locus_tag="PWY43_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135183.1"
                     /translation="MKRALKFFGIATVLSVAAIYYFLMPSHYLATPINWILPKAWKVQ
                     LPQGLNLNWQGSSLAQFQLSYRDCPLLSADKTDLGWIEKNHLFCR"
     gene            complement(2573..3388)
                     /locus_tag="PWY43_000267"
     CDS             complement(2573..3388)
                     /locus_tag="PWY43_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135184.1"
                     /translation="MAHDHDSPSKLEIATHAALPMVLMLFIEMALNFYASPDNAIFIS
                     PYLLAFFVTSFIAILVLWKGEICRGQTSRLTFILPFLLIFAVGNFIYSIAFTPKYNPM
                     AIASFAAILSCLIYFRLPEDETLYNTLMYCGFGIVAIGIIQYLAIYWIELPSLFNGIR
                     ANNFAQVLLGILLAGWYLMLAKSRLDIFLKLLVKTALVVLILNYCWTAFVLYQHLQIM
                     PDMPLLSFSIYFAAQFVILLMLAWLLLGKNIKNPTAWTVATLLALLYPFANVV"
     gene            3480..3887
                     /gene="gloA"
                     /locus_tag="PWY43_000268"
     CDS             3480..3887
                     /gene="gloA"
                     /locus_tag="PWY43_000268"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL2135185.1"
                     /translation="MRILHTMLRVGDLERSIKFYTEVLGMRLLRRSENEQYKYSLAFL
                     GYADESESAVIELTYNWGVDSYELGTAYGHIALGVDDIYQTIEDVRAAGGKITREPGP
                     VLGGTTVIAFAEDPDGYKIEFIENKNAQAALGN"
     gene            3970..4635
                     /gene="rnt"
                     /locus_tag="PWY43_000269"
     CDS             3970..4635
                     /gene="rnt"
                     /locus_tag="PWY43_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDL2135186.1"
                     /translation="MENITESTSQSTTPPTDYNLLKNRFRGYLPVVIDVETAGLNAQT
                     DALLELAAITVKMDENGYLVEDQKCHFHIKPFEGANINPESLKFNGIDIDNPLRGAVA
                     ENIAIPEMFKMVRRAMKEQGCQRAVIVAHNAAFDQGFLQAAVKRINAKRDPFHPFAMF
                     DTATLAGFMYGQTVLVKACQAAQISFDGHQAHCALYDTEKTTELFCAMVNKLKDLGGF
                     PHI"
     gene            4770..6548
                     /locus_tag="PWY43_000270"
     CDS             4770..6548
                     /locus_tag="PWY43_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL2135187.1"
                     /translation="MFKIKNSVILLNAVLLSACTSNGGSFDTNSVKASPSHSSSSKDS
                     ATQPKSERRTISSAEGSAQPSLGFQTKIPRRNFFGGTGEENGAIGEITPIVGEPGKPL
                     SLEEEMKKLEEEVKKLGYDEDDIIHTHDGKKLHRKREMKFVRSGYFIHLGASAERSKR
                     GEPRLLKTGPTGYVYYLGTQPSKAFPTEIATYKGTWDFTTNASAKNNSKYFEGSNAGT
                     NVGATSEDNQANTDDKDKPIGHSSEFTVNFSEKTLKGTLTKNGYVNPREKDKQEITDH
                     YKIEANIHGNRFNGKAKALKTDDPYFGKDSNTVEGGFYGDKAQELAGKFLADDKSIFV
                     VFAGKRDEKSEDKVEKKFDSVQVSLKDLAKSDMDTFGLATHLVIDGVQIALLPEEKTR
                     FADMDFYQVLTKNINGKDYKISVCCNNLDYVKFGTFEQDSQDNKDGHQYLVGERTAVA
                     DLPKEARIYRYTGTWDGIIRSKNGGVGGDSPSDKAHGTRSIFDVNFADKKIEGKLIAN
                     DGLETNPMLTLDGKIERNGFSGTAKTGEKGFNIDNKSTAGGTVVHLNAKFEGGFYGPQ
                     AAELGGIVHSAETNKDKVSITFGGKR"
     gene            6636..9434
                     /locus_tag="PWY43_000271"
     CDS             6636..9434
                     /locus_tag="PWY43_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HDL2135188.1"
                     /translation="MKEHTFHYSPIALAVIFSVSHSQLFAKQHQMANDHSAVLDEVVV
                     TESHYAHERQNEVTGLGKVVKNYHEMSKNQILGIRDLTRYDPGISVVEQGRGASSGYA
                     IRGVDKNRVSLLVDGLPQAQHYHTLGSDANGGAINEIEYENIRSIELSKGASSAEYGS
                     GALGGAIGFRTKDAQDIIKEGQHWGLDSKTSYASKNSHFLQSIAAAGEAGGFEALVIA
                     THRHGKETKIHSEANKLKHNIRRITGFENRYDLYPDSTQNAPGGSFFIVEDTCPTLDC
                     TPRARPKLNRDNFPVRTFPEYTPEERKQLEQIPYRTEQLSAQEYTGKDRIAPNPLDYK
                     SNSVFMKFGYHFNSSHYLGAILEDTKQRYDIRDMQTPAYYTKDDINIRVDNLSRKNSS
                     VYEGDNILDGLVFDRRIPYGLRYSHVKFFDERHHKRRLGFTYKYKPENNRWLDSIKLS
                     ADKQDIELYSRLHRLHCSDYPVVDKNCRPTLDKSWSMYRTERNNYQEKHRVIHLEFDK
                     ALNAGQGVFKQTHKLNLGLGFDRFNSLMDHGDITAQYTKGGYTSYRGRGRLDDPYIYR
                     RNPRSIETVSLCNNTHGDIINCEPRKIKGGSHFVSFRDLITSEYVDLGLGVRFDQHRF
                     KSDDPWTLSRTYRNWSWNGGITLKPTEFMSLSYRISNGFRVPAFYELYGKRDHIGLKD
                     NEYVQREQRSHQLEPEKSTNHEIGVSFKGQFGYLDVSYFRNNYKNMIATACKRIIQKS
                     HCFSNYHNIQDVALNGINLVAKFDLHGILSMLPDGFYSSVAYNRVKVKERKLNDSRLD
                     SVNDPILDAIQPARYVLGFGYDHPEEKWGIGITTTYSKAKNADEVVGTRHHGIYRVDL
                     GGKLTGSWYTHDITGYINYKNYTLRGGIYNVTNRKYSTWESVRQSGVNAVNQDRGSNY
                     TRFAAPGRNFSLAFEMKF"
     gene            complement(9539..9835)
                     /gene="fis"
                     /locus_tag="PWY43_000272"
     CDS             complement(9539..9835)
                     /gene="fis"
                     /locus_tag="PWY43_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDL2135189.1"
                     /translation="MLEQQPTQNPLTVTMLNAQAQQVNKPLRDNVKAALKNYLSQLNG
                     EDPTELYELVLSEIEHPMLDMVMQYTRGNQTRAATMLGINRGTLRKKLKKYGMG"
     gene            complement(9840..10829)
                     /gene="dusB"
                     /locus_tag="PWY43_000273"
     CDS             complement(9840..10829)
                     /gene="dusB"
                     /locus_tag="PWY43_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL2135190.1"
                     /translation="MQIGQYAIKNRIFLAPMAGITDQPFRRLCSQLGAGLTFSEMMST
                     NPDVWHTEKSRLRLAHHDEIGLNAVQIAGSDPVEMAKAAKINVEYGADIIDINMGCPA
                     KKVNKNMAGSALLRNPKLVAQILDSVVNAVDVPVTLKIRTGWDQENRNCLQIAKIAEQ
                     AGIKALTIHGRTRSCLFNGEAEYESIKAVKEAISIPVIANGDIDSAEKAKFVLDYTKA
                     DAIMIGRASFGNPWIFKEINDFFETGLSSPLSIDKKYQLMFKHIEDLHQFYGEEKGYR
                     IARKHVGWYTEKLNPESNFKRTFNTLSSTKEQLYALEDFVKSIHLESQLRKVG"
     gene            complement(11053..11541)
                     /gene="sixA"
                     /locus_tag="PWY43_000274"
     CDS             complement(11053..11541)
                     /gene="sixA"
                     /locus_tag="PWY43_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDL2135191.1"
                     /translation="MNIWIMRHGEAGFNASSDAARCLTDNGIKNTISQGKWLGEHLAN
                     QQIQLDKILVSPYLRAQQTLENVLIGMQAVNFPQHFTNLIESWDEVTPDGNPHTVESY
                     LHFLREEGAKNILLISHLPLVFDLVQILTNNQSNVQFPTSTIAEINWVKTKAELIRVK
                     HT"
     gene            11668..13107
                     /gene="pyk"
                     /locus_tag="PWY43_000275"
     CDS             11668..13107
                     /gene="pyk"
                     /locus_tag="PWY43_000275"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2135192.1"
                     /translation="MSRKLRRTKIVCTMGPATDRGNTLEKIIAAGANMVRMNFSHGVP
                     EDHIERANKVREIAARLGRHVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPR
                     GEGHQEAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLEVEDVKVHTEVTVGGPLSNNK
                     GINKLGGGLSAPALTDKDKEDIKLAAKIGVDYLAVSFPQSSADLHYARELAKEAGLEA
                     KIVAKVERAETVATEEAMDDIILGADVVMVARGDLGVEIGDAALVGVQKRLIRRARKL
                     NRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGETANGDYPVETVKSM
                     AEVCLGAETMPSINISRHRMEGTFKSIEEAVAMSAMYTANHLEGVSAIISLTHSGETA
                     KLTSRISSGLPIYAMSRNQKALNLCALYRGVTPVYYNEDSRTMEGAKKAIAQLKEQGY
                     LLTGELVLLTHGDELKAGGTNTCRILRVE"
     gene            13203..14240
                     /locus_tag="PWY43_000276"
     CDS             13203..14240
                     /locus_tag="PWY43_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL2135193.1"
                     /translation="MHNKLLSICILRLSAIGDVCHTLAVIQAIQRKYPSAEITWIIGK
                     TEAYLMKDIPNVHLIPYDKKIGWKGIFTLWQKLSHKRFDFLLNLQTAFRASILSLGIK
                     AKKKIGFNQDRAREMQWLFTNQKVEMTDSPHVLDGQMMFAKAIGVMDLSAQWNLPVSD
                     EDLAYGKQFIDFTRKNILIAPCSSKKEKDWLPERYAEIANWLSKQNINVLIAGSPSQY
                     EMETAAKIQQLAPNCTSIAGKTTLKQLAGVIRQVDLVISSDSGPAHIATTQNTPVIGL
                     YAIHNPRRTGPYNDLDKVVSVYDEAILQSYGKPWQQLAWATKAKGKNWMEKITVESVK
                     QKVVETLKITL"
     gene            14280..15338
                     /locus_tag="PWY43_000277"
                     /pseudo
     CDS             14280..15338
                     /locus_tag="PWY43_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600235.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
     gene            15393..16514
                     /gene="tyrA"
                     /locus_tag="PWY43_000278"
     CDS             15393..16514
                     /gene="tyrA"
                     /locus_tag="PWY43_000278"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDL2135194.1"
                     /translation="MNPLAPLREQIDQVDQQLIALLSERLALVAEVGKVKSEHGIPVY
                     APERETAMISARRSEAEKQGIPADLIEDVLRRIMRESYANENKHGFKRVNSDIQKIVI
                     VGGKGKLGGLFGNYLTLSGYNVEALGRDDWDNAEQILANADVVIVCVPIANTLATIER
                     LQSYLTENMILADLTSVKHQPLQKMLEVHNGAVVGLHPMFGPDIASQAKQVVACCHGR
                     FPERYQWLLEQIKIWGAKIEEIEAAAHDNAMTYIQALRHFSTFAFGLHLSRQPVQLSQ
                     LLALSSPIYRLELAMIGRLFAQDGSLYADIIADKPENLATIETLKESFEQGLDFFKRN
                     DKAGFIKAFEEVHHWFGDYSEQFLKESRVLLQQAHDSRK"
     gene            complement(16705..17790)
                     /gene="recF"
                     /locus_tag="PWY43_000279"
     CDS             complement(16705..17790)
                     /gene="recF"
                     /locus_tag="PWY43_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL2135195.1"
                     /translation="MALTRLLINHFRNIQHTDLAFSPHFNFLVGANGSGKTSLLESIF
                     YLGHGRSFKSHISNRIIHYDKDDFVLHGKIDEAKHSWSVGIQKFRSGETTLKINGEDG
                     NKIADLAHLLPMQVITPEGLTLLNGGPSFRRAFLDWGLFHQHPDFYAHWNNLRRLLKQ
                     RNSALQQVRSYQELKAWDIELVKTTYAVSEMRAEYAEALRPEIEKTCQFFLPEIEIGL
                     SFHQGWEKGADYAEILAQGFERDKALGYTMIGAQKADFRFKANGLPVEDVLSRGQLKL
                     LMCALRLAQGEHLVAQKQRQCLFLIDDFASELDPTKRELLAHRLRESGSQVFVTAITQ
                     DQLNQMQWQDRSDDCLFTVKNGEIQPQ"
     gene            17930..18259
                     /locus_tag="PWY43_000280"
     CDS             17930..18259
                     /locus_tag="PWY43_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL2135196.1"
                     /translation="MFGKGGLGGLMKQAQQMQERMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRIEIDPSLMEDNKEMVEDLVAAAFNDAVRRAEEMQKEKMASVTAGMQLP
                     PGMKFPF"
     gene            18267..18872
                     /gene="recR"
                     /locus_tag="PWY43_000281"
     CDS             18267..18872
                     /gene="recR"
                     /locus_tag="PWY43_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL2135197.1"
                     /translation="MQTSPLLENLMEALRALPGVGPKSAQRMAYHLLQRNRSGGVALS
                     KALNEAMSHIGHCKSCRTFTEEEECTICKNPRRQMSGQLCVVEMPEDIQAIEQTGQFS
                     GRYFVLMGHLSPIDGIGPREIGLDLLQQRLEQESFHEVILATNPTIEGDATANYIAEM
                     CRMYNVKATRIAHGVPVGGSLEMVDGTTLSHSFAGRRDIDI"
     gene            18944..19270
                     /gene="grxD"
                     /locus_tag="PWY43_000282"
     CDS             18944..19270
                     /gene="grxD"
                     /locus_tag="PWY43_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HDL2135198.1"
                     /translation="METIDKIKQQISENPILLYMKGSPKFPSCGFSARAVEAVINCQV
                     PFGYVDILTNPDIRAELPKFANWPTFPQLWVEGELVGGCDIVLEMLQKGELQTLLKET
                     AAKHAA"
     gene            19412..20449
                     /locus_tag="PWY43_000283"
     CDS             19412..20449
                     /locus_tag="PWY43_000283"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL2135199.1"
                     /translation="MALKELDWANLGFSYIKTDYRFIAHWKDGKWNEGELTQDNTLHI
                     HEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAERMQRTADRLLMPRVPTELFIR
                     ACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKAAPEFIFSVFCCPVGAYFKG
                     GLAPSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAVEQSSTTRKFADAIYLDPK
                     THTKIEEVGAANFFGITKDNKFITPISESILPSITKYSLLHLAQERLGMEAIEGDVYI
                     DQLDQFAEAGACGTAAVITPVGGIQHKDKFHVFYSETEVGPVTRRLYAELTGIQFGDV
                     EAPQGWIVKVE"
     gene            20476..21024
                     /locus_tag="PWY43_000284"
     CDS             20476..21024
                     /locus_tag="PWY43_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HDL2135200.1"
                     /translation="MAKLFANSTACLYLENMKKLIFLGLSALALAGCSSTISNPNQLI
                     GQWQCTIEYDDFNIRTVDNLRFSTNGTMTNQGGIYYPIQKSIFEYSLQQNGRWSVKNG
                     AIVYRIVSESVQRSHRSQIWSELQRDAELQQFEENLFSSLSDSDNNKTVELIITDFDE
                     KEMDIKQEIKGHKAYKGQCIKQ"
     gene            21033..21377
                     /locus_tag="PWY43_000285"
     CDS             21033..21377
                     /locus_tag="PWY43_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL2135201.1"
                     /translation="MIQRFEVGSRFSEMAIHNGVAYLAGQVPLDDTADAYTQTQQVLA
                     EIDKWLAKANSDKSKILMATVYLKEMADYAEMNRAWDEWVAPNNAPPRAAVEAKLANP
                     NWKVEIVVTAAV"
     gene            complement(21410..23188)
                     /locus_tag="PWY43_000286"
     CDS             complement(21410..23188)
                     /locus_tag="PWY43_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDL2135202.1"
                     /translation="MSTLDFHFIHRFHQQVQALSHHPALRFMQDEKWSEISWLELQKQ
                     VNNLSYALLTNGLAVQEKIAIFAQNMPRWTITDIAAMQIRAVAVPIYATSTTKQLEYI
                     LNDGDIKILFVGDQEQYDQAIEIANNCSLLIKIVAMKDSIDLRDFPIAQHWQDFIKVN
                     QDEAELTKRLNDKCLEDLFTLIYTSGTTGEPKGVMLDYANLAHQLQAHNKALPHIDEN
                     DISLSFLPLSHIFERAWVTYVLYRGAVNCYLEDPQQIRQALSTVRPTLMCAVPRFYEK
                     IYTAVWDKVQQAPIHRRALFKWAIRTAEDNLFGKKSAFFHRLQYIMADKLVLGKLRAL
                     LGGRIRMMPCGGAKLDPSIGLFFQSIGINVKLGYGMTETTATISCWQEKGFNINSTGT
                     LMPDVEVKISEENEILVRGGLVMKGYYKKPEATAQAFTAEGFLKTGDAGELDSEGNLY
                     ITDRIKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEE
                     YAKKLNIRYQDRLELIKHYEVVRMFEQRINELQKDLASFEQIKKFTLLSQAFSTKMEE
                     ITPTLKLRRKVILERYKQQIEKMYES"
     gene            complement(23414..23983)
                     /locus_tag="PWY43_000287"
     CDS             complement(23414..23983)
                     /locus_tag="PWY43_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDL2135203.1"
                     /translation="MNQENHKIEDIIRIFNQCFANEYNTRLERGGNYPIYLPEFMDED
                     GVPSERPYNVILFAHGYYSSALHEIAHWLVAGEARRKLEDFGYWYEPDGRSAERQREF
                     ESAEIKPQAIEWVLATAAGFRYFASADNLSGNPGDNSAFKQAVYDQVKLYAERGYLPK
                     RAETLRKALCAFYGTPDVIDLAQFDIERI"
     gene            complement(23993..25756)
                     /locus_tag="PWY43_000288"
     CDS             complement(23993..25756)
                     /locus_tag="PWY43_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDL2135204.1"
                     /translation="MIRYFFSLVLSLLMINSSHANLFSQPKFLKSEEAFVFSIQNENE
                     TLQLNWDIADNYYLYKKEIRVTPQNIEIGDITFPAAEQYNDEFFGQVEIYRNHLGLKV
                     PFKEIKDNASIAVEYQGCTKGFCYPPERVELKLDSILSATPEELAQLEAQAVEKSENF
                     AKTDNMAPPENVAKAEQDQLAENLANNRFSIFWFFVLGVGLAFTPCVLPMLPLLSAIV
                     IGNKQRPSTAKALLLSIAYVQGMAVTYTLLGLIVAAIGLPFQIALQSPPVLIGLSIVF
                     ILLACSMFGLYEIQLPNSWQQKLNAMSQKQQGGALGSVFLMGMIAGLIASPCTSAPLS
                     GALLYVAQSGDLVTGGLALYLLALGMGLPLILITLFGNKILPKSGEWLLKVKAAFGFV
                     MLALPVFLLSRVLPSHYEPFLWSALAVAFLIWLVDAIPSNSITKKIIKVLLLLALAIA
                     AKPWADLAWNGSTTQQQTTPHLALEKIQSLADLETKLTASKGKKVMLDLYADWCVACK
                     EFEKYTFSDQNVQNKLNEMVVLQIDMTKNSAENIELMKHFNVLGLPTILFFDENGNEM
                     SQSRVTGFLDAEQFLAWLNKL"
     gene            25965..26699
                     /gene="artP"
                     /locus_tag="PWY43_000289"
     CDS             25965..26699
                     /gene="artP"
                     /locus_tag="PWY43_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDL2135205.1"
                     /translation="MAIRIQNVNFFYGSNQALFDINLEIEKGDTVVLLGPSGAGKSTL
                     IRTLNLMEVPQSGTLAIANHQFDLTAKTDSKAVSLLRREVGMVFQQYHLWPHLTVMQN
                     LIEAPMKVLGLSKEEAVARAKGHLARLRLSEFAERFPLHLSGGQQQRVAIARALMMQP
                     QVLLFDEPTAALDPEITAQVVDIIKELQSTGITQVIVTHEVGVARKVASKVVYMEKGT
                     IIEQGDASCFESPKTEQFAQYLSHSE"
     gene            26727..27452
                     /locus_tag="PWY43_000290"
     CDS             26727..27452
                     /locus_tag="PWY43_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2135206.1"
                     /translation="MKKLLLASAIAFATLSANAQETITFAMEPSYAPFETTNEKGEVI
                     GFDVDIAKALCEELKATCEFKTQSFDSLIAGLKFKNFDAIISGMDITEVRQKQVAFTN
                     AYLENSVSYVAMKGKFTPETAKNVGVQNGTTFQQYLSKNAKQYNLKPYASLQSAVLDL
                     KNGRVDMVFGDTPVLAEWLKTEKELDFVGEKVVDKAFFGNGYGIAVNKSNTALLEKLN
                     SALATIKTNGKYEQIYNTWMAGK"
     gene            27452..28123
                     /gene="artQ"
                     /locus_tag="PWY43_000291"
     CDS             27452..28123
                     /gene="artQ"
                     /locus_tag="PWY43_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDL2135207.1"
                     /translation="MSSDILNLLVNATLMTLGIALASLLIGMLLSIFFVVLETSKIAC
                     IRKPTSIIITLLRGLPEILVVFLIYFGLIQVIFLITDEFAELNAFWCGAIALGIIFAA
                     YASQSLRGAIKAIPVGQWESGTALGLSRAQVFVRIILPQVWRHALPGLSNQWLVLLKD
                     TALVSLIGVDDLMRQSELINANTHQPFTWYGIAALIYLAITLISQRVIKVLEGRFTRF
                     ERGVA"
     gene            28123..28806
                     /gene="artM"
                     /locus_tag="PWY43_000292"
     CDS             28123..28806
                     /gene="artM"
                     /locus_tag="PWY43_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDL2135208.1"
                     /translation="MWWDYLVEISQGIGTSLQLTFVALIIAFILAIILTFILSLENRP
                     LKWLVNGFITLFTGTPLLIQFFLIYSGPGQFEWITESAAWVLLSDAWFCAMLALALNS
                     AAYSTLLFHGAVKAIPKGQWETCAALGLSRLDTLKILVPYALKRALPSYSNEIVLVFK
                     GTSLASTITIMDIMGYARQLYGTEYDALSIYGMAGVIYLVITGIMTIVLRKLESRVLA
                     FERFETEKA"
     gene            complement(28905..29909)
                     /gene="ruvB"
                     /locus_tag="PWY43_000293"
     CDS             complement(28905..29909)
                     /gene="ruvB"
                     /locus_tag="PWY43_000293"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL2135209.1"
                     /translation="MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVRDQMEIF
                     IKAAKLREEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLT
                     NLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTLIVKRSADCLNLNLSADGAYEVARRSRG
                     TPRIANRLLRRVRDYADVRNNGIITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERF
                     DGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGITQID
                     "
     gene            complement(30046..30651)
                     /gene="ruvA"
                     /locus_tag="PWY43_000294"
     CDS             complement(30046..30651)
                     /gene="ruvA"
                     /locus_tag="PWY43_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL2135210.1"
                     /translation="MIGRLHGKIIEKQPPEILLDVQGVGYELLLPMTSFYSLPQIGEE
                     ATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFATAI
                     ENEELAKLTKIPGIGRKTAERLLVELKGKFKRMAQSDFFVEVSHEQIVASNTPDPANE
                     ALDALIALGYKPADAEKMIKKVNKSGATSEQLIREALKNSL"
     gene            complement(30763..31386)
                     /locus_tag="PWY43_000295"
     CDS             complement(30763..31386)
                     /locus_tag="PWY43_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDL2135211.1"
                     /translation="MLTILFIHFAGLISPGPDFFYVVRQSASHSVKSGVLAATGISIG
                     IIFWAAFAIFGLAWLSNSIGSLFQYAIMIIGGCYLVYIGSQMVRVTENIIFVENKAQL
                     TPLNPWQEIKKGLLINIFNAKAGVYFTSVISAYVDNLSNTLQMFELLLLFVVSTFIYF
                     CVVALLFSRRPVRLFYAKYSRYIDNISGVIFILFGSMLIYEGFSHFI"
     gene            complement(31393..31866)
                     /locus_tag="PWY43_000296"
     CDS             complement(31393..31866)
                     /locus_tag="PWY43_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL2135212.1"
                     /translation="MKPINLKNPIHFFAFGFGSGLLKPAPGTWGSLAGLIVAIALWSI
                     TQSALFFGLLTLVSFIAGCVICTKASQDLEVHDDGRIVWDEIVAIFLMFTCLPEYNLL
                     GYLLAFISFRIFDILKPFPIRYFDEKLESGLGIMFDDILAAIFALISLHLLYYFI"
     gene            complement(31871..32851)
                     /gene="thiL"
                     /locus_tag="PWY43_000297"
     CDS             complement(31871..32851)
                     /gene="thiL"
                     /locus_tag="PWY43_000297"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDL2135213.1"
                     /translation="MGEFDIIERYFNASQRPPRKDVLLSIGDDCALTSLKPNQQLAIT
                     TDTLVCGTHFLPTISPADLAYKSVASNLSDLAAMGAEPAWISLALTLPEINHDWLAEF
                     SEHFFEILDHYNVDLIGGDTTCGPLSLTITAQGIVPQDRGLFRHNAKVGDWIYVSGTL
                     GDSAGGLQLLLNQQSEQVNWNKEQSYLIQRHLRPTPRVLLGLTIASIANAAIDISDGL
                     LSDLSHILKRSDCGAVLNLENFPLSDVLLSCYSRTEAEYFALNGGEDYELCFTVPNDN
                     KAKLDKALANIGVTYTCIGQIRSAKEGLTLLRHNQPVTLPSQRGYDHFKK"
     gene            complement(32914..33327)
                     /gene="nusB"
                     /locus_tag="PWY43_000298"
     CDS             complement(32914..33327)
                     /gene="nusB"
                     /locus_tag="PWY43_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL2135214.1"
                     /translation="MKISPRRRARECAVQAIYSWYISQNTVEEVELAFVTDQDMKGVD
                     MPYFRKLFRGVVDNVEAIDEALRPYLDRKESDVDPIERSILRLSTYELKFEHDVPFRV
                     VINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK"
     gene            complement(33408..34721)
                     /gene="pilQ"
                     /locus_tag="PWY43_000299"
     CDS             complement(33408..34721)
                     /gene="pilQ"
                     /locus_tag="PWY43_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HDL2135215.1"
                     /translation="MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNM
                     VLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQEDDIVYIHKKEEKAADLTPLPI
                     MNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
                     SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGH
                     YKFGGRLEGNGLNDVTNNLNVNFPITGGASAVLQVASINSRVLDLELSALEQENSVEI
                     IASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLGLEVVPHLSTQNQIL
                     LDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPI
                     LGSIPFIKRLFSQTRDKISKRELVIFVTPYILQSNEKSSNHKKSK"
     gene            complement(34730..35143)
                     /locus_tag="PWY43_000300"
     CDS             complement(34730..35143)
                     /locus_tag="PWY43_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135216.1"
                     /translation="MRNWFLFITLFISFSVKANDPFFGEKQETQASGNIAESNVEFAK
                     NTENITACKVPDNLSAKNIVTDFDKMTLIGLVKINEVMRALFIDEKQQLLALKENDLL
                     NNEIEIKHINLKSITYINWKLTKDCNIPYEINIKL"
     gene            complement(35146..>35482)
                     /locus_tag="PWY43_000301"
     CDS             complement(35146..>35482)
                     /locus_tag="PWY43_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135217.1"
                     /translation="INFNHQQNKYDSIINKAKNQNSQENNLTEINSNIQILAEKHQLT
                     IESLQWNLEQGKSIELSIIGNSRSLFDFIHSVKQIPYLKYNAISLIKSRQDRKVEMNT
                     TLVILANKE"
BASE COUNT        10706 a         6676 c         7561 g        10539 t
ORIGIN      
        1 tgtaagtata ttttatgcaa accgtctata tcgccttagg atcaaacctt gagaatccgc
       61 ttgagcagct taaacaagcg gtcgaaaaat taaaaaaatt ggcaaatgag tttgaaatca
      121 gcccgttcta cggctcaaag cctgtcggac cgcaagatca gcctgattat gtcaatgcgg
      181 tagcaaaatt tagtaccgac ctatcgccca ctgcattgct ggataaattg caaagcattg
      241 aaaatgagca gggaagagtg cgtgtacgcc gttgggggga gagaacttta gatctggata
      301 ttattttgta cggcaatcag caaattcaaa ccgagcgttt gaacgtgccg catatcgaga
      361 tgaaaaatcg ggaatttgtg attgtgccga tgtttgattt aacgcctgat ttagtactac
      421 cgacagggga aaaattagcc gatatttatg cacaatttaa agatcatcaa atgattatct
      481 tcagctaatt tttcgttttg ttctggtttt ttggctccga aatgaaagcg tccgttttgg
      541 tagcggtagg gaagcgaaaa ttcaatccct tttgcttcgg ccattaggta aagataaacc
      601 gccgttacga atggctaaat gcccaccggc aatcagcccg tttttactgc cggcactaaa
      661 cgccgtttcg ccccgaattg taccgttgtt gatcacccat gggctacgaa acggaaaaag
      721 cggctggaag ccttgagcag attgctccgg ccaataaagt ttgccgataa tttggctggc
      781 atcttggctt aattggcggc gggcgaacag tttgagtggg ccgagttcgg tggtattcac
      841 cgctccttta aaacgtagtt tttcgacttc accatcaaag gcgagattca ctcgtggttt
      901 gggtagcaca ccgccataac tgaaggcaat ctcaggtgat tgcaacatta ccgctccgtc
      961 aagggtgaat tttttgacat taaatcgaat agcttgcgta ttgttaagcg tgattttaga
     1021 aatggttgta tctgctaact ttacggtggc taaatcgcct ttgagttctt ccaaccgcac
     1081 aagctgttta tgccattgcc ctctgccgct taaattcact ttactgttga aggctttgag
     1141 gatagatgct ccccaaattc gccatttcca actgtctttt actgcgttgg catctttagg
     1201 atcttggaaa aaatctaagg ctccagcctt aaaattttgt gcttgtccgt ctaaatggaa
     1261 ctcgatattg ttaaaatcag ggctttgccc tcttaaaatt gcgtgcagtc gtccgtgaat
     1321 accgtgcttg tcaattttaa ttccggcaag tggaaaacgt aaatcttcaa tggttaaata
     1381 ccgttctttt ccggtcaaac gaagtaggga gctaggcaaa aattttacgc ttaaatcgtc
     1441 aaaattacct tgaaaatcca gtggcacgct actgtaaaga ataaaattgc cgtgtttgat
     1501 attacccgtc aaacgaagcg gtaaattcag ccctttttcg gttatttctc cgtctgaatt
     1561 ggagattaca atattgcctt taccccaact gttttgggaa agcaggctga tacgaatcgc
     1621 cgttttgagt ggcaaaaagc cgttttggtt gaatgatttc ggtgtgagct tggtgctaac
     1681 aaacccttgc agtggaaatt gttgggtaaa gtgccaatca aaccgccctt gttgaatttc
     1741 taggctttcc ccatcaaaca gaaaagggaa atccagtttg ttattttcac tttcggcaag
     1801 ttcaacctgc cagttgcctt ctaacttttg tgcggcattt ttttgccaac tgagaataga
     1861 gcgccctttt tgcaaggctt tattggcgat gatttcatca gataattgcc aatcatattc
     1921 agcggaaata gtgggcggca gttgggtaaa attgtcctca atttctgctg aaagcctaag
     1981 attatgctgt tctttttcat tcggttgata tgataaatcg gcattcaacc gcccatttgc
     2041 aaaatttgct gcaaatttga ccgcttgttg agcggcagtt gcctgaattt caccttttga
     2101 aagtgagagt ttaatagctg tttgctcaaa aagccgctga agtcttgggt tgaaatcggt
     2161 aggcaaattt ttccagttaa gggcgtggat agtcgcttcg ccatcaggaa tgaccgccag
     2221 caatgcgttg agtgaagtgt cggaattgct gtgttcattt tgtggaaaca aggcaaggca
     2281 gcggtaatca atatccaatt tatcgacaga aaagatggtt tttctcaatc caacccaaat
     2341 cggttttatc ggcacttagt agcgggcaat ctcgataaga gagttgaaat tgagcaagtg
     2401 atgagccttg ccaattcagg tttaagcctt gtgggagttg aactttccac gctttcggca
     2461 aaatccagtt aatcggcgtg gcaagatagt ggctgggcat taaaaaatag tagattgccg
     2521 caacagatag aacggttgca atgccgaaaa attttaatgc ccgtttcata aatcagacta
     2581 cattagcgaa agggtataaa agcgctaaca gcgtagcaac tgtccaagcg gtcggatttt
     2641 tgatgttttt tccaagtaat aaccacgcca acatcaataa aatcacaaat tgtgctgcaa
     2701 aatagatgct aaatgaaagc aatggcatat ccggcataat ttgtagatgt tgataaagca
     2761 caaaagctgt ccagcaataa ttgagaatca gcaccacaag ggcggttttc accaacagtt
     2821 tgaggaaaat atccaaacgg cttttcgcta acattaaata ccagccggct aataaaatgc
     2881 ctaacagcac ttgggcgaaa ttgttggctc gaatcccgtt aaacaatgaa ggcagttcaa
     2941 tccaataaat tgctaaatat tgaataatgc cgattgccac aatgccaaaa ccacaataca
     3001 tcaacgtgtt gtagagcgtt tcatcttccg gcaaacgaaa atagatcaag caagataaaa
     3061 tggcagcaaa acttgcaatt gccattgggt tatatttagg tgtgaaagca atcgaataaa
     3121 taaaatttcc gactgcaaaa atcagtaaaa aaggcagaat aaaggttaag cggctggttt
     3181 gcccacgaca aatctcacct ttccataata ctaaaatggc gatgaaagag gtaacaaaaa
     3241 acgccaataa ataaggggag atgaaaatag cattatccgg agaggcgtaa aaattaagtg
     3301 ccatttcaat aaatagcatt aacaccatcg gcagggctgc gtgtgtggca atttcaagtt
     3361 ttgatgggct gtcgtgatcg tgagccataa ataatcctaa aaaattgaaa gaaaatgatt
     3421 ttaagctcaa attatgccat aatacgcaag attttttaac ttcaaggggg aaagaaaata
     3481 tgcgaatttt acacacaatg ttacgcgttg gagatttaga gcgttcaatc aaattctata
     3541 ccgaagtgtt aggtatgcgt ttgttacgca gaagtgagaa cgagcaatac aagtactctt
     3601 tagccttctt aggctatgct gacgaaagcg aaagtgcagt tattgagctg acttacaact
     3661 ggggtgtgga tagctacgag ctaggtactg cttatggtca tattgcatta ggtgttgatg
     3721 atatttatca aacgattgaa gatgtgcgtg cggcaggtgg taaaattacc cgtgagccgg
     3781 gtcctgtatt aggcggcaca acggttattg cttttgcgga agatccggat ggttataaaa
     3841 tcgaatttat tgaaaacaaa aacgcacaag ccgcattagg aaactaattc cttttatcta
     3901 ttcacaagcg gtcggatttg taaaaaattt tgcaaatcag accgcttgta tttttaagtt
     3961 gaaataaaaa tggaaaatat aactgaatct acctctcaat cgactacacc accaaccgat
     4021 tataatctat taaaaaatcg ttttcgtggt tatttgcccg ttgtgattga tgttgaaact
     4081 gcggggctta atgctcaaac tgatgcgtta ttagaactgg cggcaattac ggttaaaatg
     4141 gacgaaaacg gttacttagt ggaagaccaa aaatgccatt ttcatatcaa accgtttgaa
     4201 ggggcgaata ttaacccaga gtcgctaaag tttaacggca ttgatattga taacccattg
     4261 cgaggtgcgg tggcagaaaa tatcgctatt cccgaaatgt ttaaaatggt gcgtagagcg
     4321 atgaaagagc agggttgcca acgtgcggtg attgtggctc acaatgctgc gttcgatcag
     4381 ggatttttac aggcagcggt taaaaggatt aatgctaaac gtgatccctt ccaccccttt
     4441 gcaatgtttg atactgcaac ccttgcaggt tttatgtacg gacaaacggt gttagttaaa
     4501 gcctgccaag ccgcccaaat tagttttgac ggacaccaag cccactgtgc attatacgat
     4561 accgaaaaga ccacagagct attttgtgct atggtgaata aactaaaaga tttgggcggc
     4621 tttccacaca tttagtgtag aggaagatgg gcgttatttt tcaattttta ttatttaaaa
     4681 gatggctctc taaaaaaata taatctagct atttacatag ttcttctata taaataaaat
     4741 ggtaatcatt tttaattaag gagaaaattg tgtttaaaat aaaaaatagc gttattttat
     4801 tgaatgctgt actactttcg gcttgtactt caaacggtgg tagttttgat accaattcag
     4861 ttaaggcaag cccatctcat tctagctctt ctaaagactc agcaactcag cctaaatcag
     4921 aacgcagaac gatatcttcg gctgaaggct cagctcaacc atccttagga tttcaaacta
     4981 aaattccacg tcgtaatttc tttggtggca ctggagagga aaatggggcg attggtgaaa
     5041 ttacaccaat tgttggtgag ccaggcaagc cattatctct tgaagaagaa atgaaaaagc
     5101 ttgaggaaga agtaaaaaaa ctcggttatg atgaagatga cattattcac acccacgatg
     5161 gaaaaaaatt acaccgtaag cgagagatga aatttgttcg ttcgggttat ttcattcatt
     5221 taggggcttc agccgagaga agtaaaagag gagaacctcg tcttctaaaa acaggtccta
     5281 cagggtatgt atattattta ggcactcaac catctaaagc tttcccaacc gaaatagcaa
     5341 cttataaagg gacttgggat tttactacta atgcatctgc aaaaaacaac agtaagtatt
     5401 ttgaaggaag taatgctggc acgaatgttg gggcaacgtc agaagataac caagcaaata
     5461 cagatgataa ggataagcct atagggcata gcagtgagtt tacggttaat tttagtgaga
     5521 aaacacttaa aggtacttta accaaaaatg gctatgtaaa tccaagggaa aaggacaagc
     5581 aggaaattac tgatcattat aagatagaag cgaatatcca cggaaaccgc tttaatggta
     5641 aggcaaaggc tttaaaaaca gacgatcctt actttggtaa agatagtaat acagtagaag
     5701 gcggttttta tggcgataaa gcgcaggaac ttgcgggaaa atttttggca gatgataaat
     5761 cgattttcgt tgtattcgca ggtaagcgtg atgaaaaaag cgaagacaag gtcgaaaaga
     5821 aatttgattc agtgcaagtg agtttgaaag atttggctaa atcagatatg gatacttttg
     5881 gtctggcaac tcatttagtt attgatggtg ttcaaattgc tttattgcca gaagaaaaaa
     5941 cacgttttgc tgatatggat ttttatcaag tattaacaaa aaatataaat ggtaaagatt
     6001 acaaaatttc agtctgttgt aataatttag actatgttaa attcggcaca tttgaacaag
     6061 acagccaaga taataaagac gggcatcaat acttagtggg tgaacgtacg gcagttgctg
     6121 atttaccaaa agaggctcgt atttatcgtt atactggtac ttgggatgga attattcgta
     6181 gtaagaatgg gggtgtaggt ggtgattcgc caagtgataa agctcatggc actcgttcta
     6241 tatttgatgt gaattttgcc gataagaaga ttgaaggtaa attgattgcg aatgatggtt
     6301 tagagacaaa tccgatgctg acattagatg gaaagattga acgcaatggt tttagcggta
     6361 cagcaaaaac gggtgaaaaa ggctttaata ttgataataa gagtacagct ggtggtacag
     6421 ttgttcatct taatgccaaa tttgaaggtg gtttttatgg tccgcaagct gctgaattag
     6481 gcggcattgt gcatagtgca gaaactaata aagataaagt gagcatcact tttggtggaa
     6541 aacgctaggt agagaataag ccaagattta cccgctctaa caagcgggta attttcgcca
     6601 aaaaaatgtg aaatctaaag gagataaata aaacaatgaa agaacatact ttccattatt
     6661 cgccgattgc tcttgcagtc attttttccg tctcacactc tcaattattt gccaagcaac
     6721 atcaaatggc aaacgatcat tctgcagttc tcgatgaagt tgttgtgact gagagccatt
     6781 atgctcacga acgtcaaaac gaagtaactg gcttggggaa agtagtgaaa aattatcacg
     6841 aaatgagtaa aaatcaaatt cttggtattc gtgatttaac tcgctatgac cctggtattt
     6901 cggtggtgga acaaggtcgc ggtgcaagta gtggctatgc cattcgaggt gtagataaaa
     6961 accgtgtcag cttacttgtt gatgggctac cacaagcgca gcattatcat acgctaggtt
     7021 cagatgctaa tggtggtgca attaatgaga ttgagtatga aaacattcgt tcaattgagt
     7081 taagcaaagg agcaagttct gcggaatatg gctctggtgc gctaggtggt gctattggtt
     7141 ttcgtactaa agatgcgcag gatattatta aagaggggca gcattggggc ttagatagta
     7201 agacctctta tgccagcaaa aatagccatt ttttacagtc tatcgcagcg gctggtgagg
     7261 cgggtggttt tgaagcactt gttattgcaa ctcaccgaca cggtaaagag accaaaattc
     7321 attccgaggc aaataaatta aaacataata ttcggcgtat aactggcttt gaaaatcgct
     7381 atgatcttta cccagattcc acacagaatg ctcctggagg atcttttttt attgtggaag
     7441 atacttgccc aacattagat tgtactcctc gtgcaaggcc taagttgaac cgcgataatt
     7501 tcccagtgag aacatttccg gaatatacgc ctgaagagcg caaacagctt gagcagattc
     7561 cttatcgcac tgagcagctc tcagcccaag aatataccgg taaagatcgc attgcaccaa
     7621 accctttaga ttacaagagt aattctgttt ttatgaagtt tggctatcac tttaactcgt
     7681 ctcattatct tggcgcaatc ttagaagata caaaacaacg ctacgatatc cgtgatatgc
     7741 aaacgccagc ttactataca aaagacgata ttaatatcag ggtagacaac ttatcacgta
     7801 agaactcttc tgtttatgaa ggggataata ttttagatgg cttagtgttc gatagaagga
     7861 tcccttatgg gttgcgctat agccatgtga agttttttga tgaacgtcac cacaaacgtc
     7921 gtttaggatt cacctataaa tataaaccag agaataatcg ctggttggat agcattaaac
     7981 tcagtgcgga taaacaagat attgaactat atagccggct acatcgcttg cattgtagcg
     8041 attatcctgt ggtagataaa aattgccgcc cgactttgga taaatcttgg tctatgtatc
     8101 gaactgagcg taataattac caagaaaagc atcgtgtcat tcatttagaa tttgataaag
     8161 cgctaaatgc tggtcaaggc gtatttaagc aaacccacaa actgaattta gggttgggct
     8221 ttgatcgatt taattcgctt atggatcatg gggatataac tgcccaatat accaaaggcg
     8281 gttataccag ctaccgcggt agagggcgtt tagatgaccc atatatttat cgtcgcaatc
     8341 cacgcagtat tgaaacggta tctttgtgta ataatacaca cggcgacatc ataaactgtg
     8401 aaccgcgtaa aattaaaggc ggtagccatt ttgttagctt ccgcgatctg ataacgagcg
     8461 agtatgtgga tttgggatta ggggtgcgtt ttgatcaaca tcgatttaaa tcggatgacc
     8521 cgtggacact tagccgaact tatcgaaatt ggtcttggaa tggcgggatt acgcttaaac
     8581 cgactgagtt tatgtcgctt tcttaccgca tttcaaacgg ttttagagtg cctgcattct
     8641 atgaacttta tggtaaacgt gatcatattg ggcttaaaga taacgaatat gtgcaacgcg
     8701 agcaacgtag ccaccagtta gagccagaaa aatcgactaa tcatgagatt ggagttagct
     8761 ttaaaggtca atttggttac cttgatgtga gctatttccg taataactat aaaaatatga
     8821 ttgcgacagc atgtaaaaga ataatacaaa aatcacactg tttctctaac taccataata
     8881 ttcaagatgt agcactaaac gggataaatt tagtcgctaa atttgactta cacggtattt
     8941 tatctatgct gccagatggt ttttattcat cagttgctta taaccgtgta aaagtaaaag
     9001 agcggaaact aaacgactca agactcgata gcgtaaacga tcctattcta gatgcgattc
     9061 agccagcacg ctatgtgctt ggattcggct acgatcaccc agaagaaaaa tggggaattg
     9121 gcattactac cacctattct aaagccaaaa acgctgatga ggtggtaggc acacgtcatc
     9181 acggcatata tcgcgttgat ttaggtggca aactgaccgg ttcttggtac acccatgata
     9241 ttaccggtta catcaattat aaaaactaca ccttacgtgg aggaatttat aatgtgacta
     9301 atcgtaaata ttccacttgg gaatcagtgc gccaatccgg tgtgaatgca gtaaaccaag
     9361 accggggtag caattacact cgatttgccg ctccggggag aaatttcagt ttagcatttg
     9421 aaatgaagtt ttagacggca aaataaaata gaaaaacgct gaatagtata ttcagcgttt
     9481 tttgttggct ctaactaatt acatacgaat taaaatcgtg ttttaacggt cggttagatt
     9541 aacccatacc gtattttttt aattttttgc gtaatgtgcc acggttaatg cctagcattg
     9601 ttgctgcacg agtttgattg ccgcgagtat attgcatcac catatctaac attgggtgtt
     9661 cgatttcaga taatactaat tcgtataatt ctgttggatc ttcaccattt aattgagata
     9721 aatagttttt taatgctgct ttcacattat cgcgaagtgg tttattaacc tgctgcgctt
     9781 gtgcatttaa cattgttact gttaatgggt tttgtgtagg ttgttgctct aacatttttt
     9841 tatccaactt ttcttaattg agattctaaa tgaatcgatt taacaaaatc ttctaatgca
     9901 taaagttgct cttttgtgga acttagtgta ttaaaagtgc gtttaaaatt tgattccgga
     9961 tttaattttt cggtatacca accaacatgt tttcgtgcaa tacgataacc tttttcttca
    10021 ccataaaatt ggtgcaaatc ctcaatatgt ttaaacatta attggtattt tttatcaatt
    10081 gataatggtg aagataatcc agtttcaaaa aaatcattaa tttccttaaa tatccaagga
    10141 tttccaaagc tagcgcgccc aatcatgatt gcatcggctt tggtatagtc gagaacaaac
    10201 tttgcttttt ccgcagagtc aatgtctccg tttgctatca ctgggataga aattgcttct
    10261 tttactgcct taatactttc atattcagct tcgccattaa ataaacagct tcgagtacgc
    10321 ccgtgaatag tgagagcttt tatgccggct tgttctgcaa ttttcgcaat ttgcagacaa
    10381 tttctatttt cctgatccca acctgttcta atttttagtg tcacagggac atcaacggca
    10441 ttgactacag aatctaatat ttgagcgact aatttggggt tacgtaaaag agcagagcct
    10501 gccatatttt tattcacctt ttttgccggg caacccatat taatatcaat aatatcggca
    10561 ccatactcaa catttatctt tgcagccttt gccatttcaa caggatcaga acctgcaatt
    10621 tgtacggcat ttaagcctat ttcatcgtgg tgtgctaacc gcaaccgtga tttctcagta
    10681 tgccatacat caggattggt agacatcatc tctgaaaatg ttaaacctgc tccaagttgg
    10741 ctacataagc ggcgaaaagg ctgatctgtg attccagcca taggtgcaag gaaaatacga
    10801 tttttgattg catattgtcc tatttgcatt tttaaccttt tattaacaaa tttgaagtaa
    10861 ttttaaccag agggatatcc aaataaaaag tacggtaaat accgtacttt cttataatag
    10921 cttatggatc agtttagtac cgatttgagg gctggtatga tacgcatttt ttaggtattt
    10981 tggaagtcta taaattcgac aatttataaa aaaattgaaa atttttatat tgacattcta
    11041 aaaatgaaag aattaagtat gctttactcg aattagctca gcttttgtct ttacccaatt
    11101 aatttctgct atggttgaag ttggaaattg tacattgctt tggttattcg ttaaaatttg
    11161 gacgagatcg aacacaagtg ggagatgtga aatcagaaga atattttttg cgccttcctc
    11221 tcttaaaaaa tgtagatagc tttctactgt atgcgggttt ccgtcaggcg taacctcgtc
    11281 ccaactttca atgaggttgg taaaatgttg gggaaaattg accgcttgca taccaataag
    11341 cacattttca agcgtttgtt gcgccctaag gtatgggctg acgagaattt tatctaattg
    11401 aatttgttgg ttggctaaat gttctcctaa ccattttccc tgtgaaatgg tgttttttat
    11461 accattgtcg gttaaacagc gagcagcatc actcgaagca ttaaagcctg cttccccatg
    11521 gcgcataatc caaatattca taacattctc ctttagttgt atttaaactc tcttgattta
    11581 ggcgtataat gaccctcgtt taacgcataa tcaagatgct tagaaagatt ttaacttaat
    11641 ttttaaattt attgatggag tcaaataatg tctagaaaac tcagaagaac gaagatcgtt
    11701 tgtacaatgg gccctgctac cgaccgtggc aatactttag aaaaaatcat tgctgccggt
    11761 gcaaatatgg ttcgtatgaa cttctcccac ggtgttcctg aagatcacat tgaacgtgcg
    11821 aataaagtgc gtgaaattgc tgcaagatta ggtcgtcatg ttgcgatttt gggtgattta
    11881 caaggtccta aaattcgtgt ttcgacattt aaagacggta aaattttctt aaatattggt
    11941 gataaattca ctttagacgc agatttaccg cgtggagaag gccatcaaga agctgtaggt
    12001 ttagattaca aaaacttacc aaatgatgta gtacctggtg atattctatt gttagatgac
    12061 ggtaatgttc aactgaaagt gttggaagtg gaagacgtta aagtccatac tgaagtgaca
    12121 gttggtggcc cgttatcaaa caataaaggg attaacaaat taggtggtgg tttatcagct
    12181 cctgcattaa ccgacaaaga taaagaagac attaaattag cggcgaaaat cggtgtggat
    12241 tacctagcgg tttcattccc tcaatcaagt gcagatttac attatgcacg tgaattagca
    12301 aaagaagcag gcttagaagc gaaaattgta gcgaaggttg aacgtgccga aactgttgcg
    12361 actgaagaag caatggacga catcatttta ggtgcggacg ttgtgatggt tgctcgtggt
    12421 gacttagggg ttgaaattgg tgatgcggct ttagtgggtg tgcaaaaacg tttaatccgc
    12481 cgtgctcgta agctaaatcg tgtggtaatt accgcaaccc aaatgatgga atcgatgatt
    12541 aaaaaaccaa tgccgacccg tgctgaagtg atggatgttg caaatgctgt gttagatggt
    12601 actgatgcgg taatgctttc cggcgaaact gcaaacggtg actaccctgt tgaaacggta
    12661 aaatcaatgg cagaagtgtg cttaggtgct gaaacaatgc caagcattaa catttctcgc
    12721 caccgtatgg aaggtacgtt taaatctatc gaagaagctg tggcaatgtc tgcaatgtac
    12781 accgcaaacc atttagaagg tgtatcggca attatttcat taactcattc aggcgaaacg
    12841 gctaaattaa ccagccgtat cagctcaggt ttaccgattt atgcgatgtc tcgtaaccaa
    12901 aaagcattaa acctttgtgc gttatatcgt ggtgtaacac cggtatatta caatgaagac
    12961 agccgcacaa tggaaggggc gaaaaaagcc attgctcaat taaaagaaca aggctattta
    13021 ttaaccggcg aattagtgct tttaactcac ggagatgaat taaaagctgg cggtacaaat
    13081 acttgtcgta tcttaagagt tgaataatct aaacgattaa cgcaaaagcc acataaaaca
    13141 ctttatgtgg ctttttttct gtcttaatat agcttaatgt aatctgaact attgatatta
    13201 ttatgcacaa taaacttctt tctatctgta ttctccgcct ttctgccatt ggcgacgttt
    13261 gccatacctt agcggttatt caagctattc aacgaaaata tcctagtgct gaaattactt
    13321 ggattatcgg caaaacggaa gcgtatttaa tgaaagatat tccgaatgtt cacttaattc
    13381 cttatgataa aaaaataggg tggaaaggta tctttacact ttggcaaaaa ctttctcaca
    13441 agcggtttga ttttttatta aatttacaaa cggcattccg agcttcaatt ctatcgcttg
    13501 ggattaaagc aaagaaaaaa atcggcttta atcaagacag agcaagggaa atgcaatggt
    13561 tgtttaccaa ccagaaagta gaaatgacag actctcctca cgtgcttgac gggcaaatga
    13621 tgtttgccaa agccattggg gtaatggatt tatcggcaca atggaattta cctgtgagtg
    13681 atgaagattt agcttatggc aaacaattta ttgattttac aaggaaaaat atactcattg
    13741 ctccttgttc aagcaagaaa gagaaagatt ggttgccgga acgctatgca gaaattgcta
    13801 actggctaag taagcaaaat atcaatgtat taattgcagg ttctccatcg caatatgaaa
    13861 tggaaaccgc agccaaaatt caacaacttg ccccgaattg tacgagtatt gccggtaaaa
    13921 ccacgctaaa acagcttgca ggtgttattc gtcaggttga tttggtgatc tcttcagatt
    13981 caggtcctgc ccatatcgcc actacgcaaa atacgccggt gattgggttg tatgcgattc
    14041 ataatccaag acggacaggc ccttacaatg acttagacaa agtggtttcg gtatatgatg
    14101 aagcgatttt gcaatcttat ggtaaacctt ggcagcaatt agcttgggca actaaagcta
    14161 aaggcaaaaa ttggatggaa aaaatcacgg tggagagtgt gaaacaaaaa gtagttgaaa
    14221 ctttaaaaat tacgttgtaa agtttgtgta ttaaaagatt tacacaaaag gaaattaaaa
    14281 tgaacacagt gtttaatcaa gatagcgtgc ataacgtaaa tatccaagat gaaaaggtat
    14341 tactgacccc acaagagtta aaacaagaat ttccgttgcc ggaacattta cgcaatcaaa
    14401 ttgaacgttc acgcaaaacg atttccgata ttattcacag acgagataaa cgccaactga
    14461 ttgtgattgg accttgttcg attcacgacc cagttgctgc ccttgaatat ggtaaaaaat
    14521 taaaagctct ttcggagcaa gtgagtgata agttgtatat tgtgatgcgg gtttattttg
    14581 aaaagccccg cactactgta ggttggaaag ggctgattaa cgatccgaaa atggacggct
    14641 cttttgatgt cgaaaccggc ttacgtatcg cacgaaaact ctgtttggat ttagccgaac
    14701 ttggcttgcc gttagcgacc gaagccttag atccgatgac accacaatat ttagcggatt
    14761 tgttcagctg gtcggcaatt ggagcaagaa caactgagtc tcaaactcac cgtgaattag
    14821 cctcaggttt gtcaatggcg gtaggcttta aaaatggtac agatggtgat ttagccgttg
    14881 cacttaatgc gatgcaatct tccgctcaaa gccatagctt tatcggcatt aaccaacagg
    14941 ggcaagttac tttactcaaa acaaaaggta atgcagacgg acatattatt ttacgtggcg
    15001 gtaaaacccc aaattatgaa aagcaatatg ttgatgattg tgaatgggtg ctaaaacaag
    15061 caaacttacc tacagcgatt atgattgatt gcagccacgg caattccaat aaagattacc
    15121 gccgccaacc gctggtggca gaaaatgtat gagagcagct acttgctggc aatacttcga
    15181 ttatcggttt aatgattgaa agcaatttac acgcaggtaa tcaatcatca gaattgccgt
    15241 tcgagcagat gaaatacggt gtttctatta cggatgcttg tattgactgg caaactacag
    15301 aagagctttt aactcacttt gctgaaaagt tgcgttaaag ataatttgca aaaaattgca
    15361 taaaaacgac cgcttgtaaa ttggtaacaa atatgaaccc attagcccca ttacgagaac
    15421 aaattgatca agttgatcaa caacttatcg ccttactttc tgaacgctta gctcttgtgg
    15481 cagaagttgg gaaagtcaaa tcagaacacg gcattcctgt ttatgctccg gagcgagaaa
    15541 ccgcaatgat ttccgcccgc cgctctgaag cggaaaaaca aggtattccg gcggatctca
    15601 ttgaagatgt gctacgccgt ataatgcgtg aatcttatgc caatgaaaac aaacacggct
    15661 tcaaaagagt taattcggac attcaaaaaa tcgtgattgt tggtggtaaa gggaaattag
    15721 gtgggctgtt tggcaattat ttaaccctat ccggctataa tgttgaggca ttaggacgtg
    15781 atgattggga caatgccgag cagattttag ccaatgcaga tgtggttatt gtgtgtgtgc
    15841 cgattgccaa caccctagca accatcgagc gtttacaatc ttatttaacc gaaaatatga
    15901 ttttggcgga tttaacctcc gtcaaacatc agccgttgca aaaaatgctg gaagtgcata
    15961 atggagcggt agtcggttta cacccgatgt tcggaccgga tattgcaagt caggcaaagc
    16021 aagtggtcgc ttgctgccac gggcgtttcc ctgaacgtta tcaatggttg cttgagcaaa
    16081 tcaaaatttg gggggcaaaa attgaagaga ttgaggcagc agcacacgat aatgcaatga
    16141 cctacattca agccctacgc cacttttcca cttttgcatt cgggctacat ctttctcgcc
    16201 agccggtgca actctctcaa ttattggcac tatcttcgcc gatttaccgc ttagagttgg
    16261 caatgattgg gcgtttattt gcccaagacg gcagcttata tgccgatatt attgccgata
    16321 aaccggaaaa tttagccacc atcgaaacct taaaagagag ttttgaacaa gggctagatt
    16381 tctttaaacg caacgacaaa gccggtttta tcaaggcttt tgaggaagtg catcactggt
    16441 ttggcgatta ttccgagcag ttcttgaaag agagtagagt attgctccaa caggctcacg
    16501 attcaagaaa gtaaaggtag gttgaggttt tgctctgata gcactagcgt ccacgctagt
    16561 gctatttaat attctacaag cggttaataa agagctgttt tttgcaaatt tttatgcaaa
    16621 attatccgct tgttttggca cgagcgtgga cgctcgcgcc atcggaagaa acatttttgt
    16681 aaaattgccc ccaaatctga ccgcttactg tggctgaatt tctccatttt ttaccgtaaa
    16741 caaacaatca tcgcttctgt cttgccattg catttgattg agctgatctt gagtaatggc
    16801 ggtaacgaat acttgtgagc cactttctct taaacgatgt gctaaaagct cacgtttggt
    16861 tgggtcgagt tcggaggcaa aatcgtcaat taaaaatagg cattggcgtt gtttttgggc
    16921 gaccaaatgt tcgccttgag ccagtcttaa cgcacacatc aatagcttta attgcccacg
    16981 ggaaagcaca tcttccaccg gtaagccgtt cgctttaaaa cggaaatcgg ctttttgagc
    17041 tccaatcatc gtatagccaa gagctttatc ccgctcaaaa ccttgtgcca gaatctcggc
    17101 ataatccgcc cctttttccc agccttgatg aaagctcaag ccgatttcga tttccggcag
    17161 gaaaaattgg caggttttct caatttcggg gcgtaacgct tcggcgtatt cggctcgcat
    17221 ttcgctcacg gcataagtgg ttttcaccaa ttcgatatcc cacgctttga gttcttgata
    17281 agagcggact tgttgcaaag ccgaattacg ttgctttaat aaacggcgaa gattgttcca
    17341 atgagcgtag aaatcagggt gctggtgaaa taagccccag tccaaaaaag cacggcgaaa
    17401 gctcggacca ccgttaagta aggttaagcc ttcgggggtg atcacttgca tcggcaataa
    17461 atgggctaaa tcggcaattt tgttgccgtc ttcaccgttg attttaagtg tcgtctcgcc
    17521 actgcgaaat ttctgaatgc cgaccgacca actgtgcttt gcctcgtcaa ttttaccgtg
    17581 tagtacgaaa tcgtctttat cgtaatggat aatgcggttg ctgatatggc ttttgaatga
    17641 acgcccgtgt ccaagataga aaattgattc caacaaactg gttttgccac ttccgttcgc
    17701 cccgactaaa aaattaaaat gcgggctaaa tgctaaatcg gtatgttgga tatttctaaa
    17761 atggttgatt aataaacggg ttaaagccat agttaaagtg cgaggaataa aaagagtagc
    17821 cattttagca aatttgcaaa attttgggaa aaattaaccg cttgcggatt agaaagtggt
    17881 tcagtagaat aagccgttta ttttttattg aaaattaaga ggaaaaatta tgtttggaaa
    17941 aggtggctta ggcggcttaa tgaaacaggc tcagcaaatg caagagcgta tgcaaaaaat
    18001 gcaagaagag atcgcccaat tagaagtaac aggcgagtct ggtgcgggtt tggtaaaagt
    18061 cactattaac ggggctcaca attgccgccg aattgaaatt gatccatcat taatggaaga
    18121 taataaagag atggtggaag atttagttgc cgctgccttt aacgatgcgg ttcgccgtgc
    18181 agaagaaatg caaaaagaga aaatggcaag tgtgactgcc ggtatgcaac tgccgccggg
    18241 tatgaaattc ccattctaat ttaagtatgc aaaccagtcc tttacttgaa aatttgatgg
    18301 aagccttgcg tgcattgccc ggtgtcggac cgaaatcggc acagcgaatg gcttaccatc
    18361 tgttgcaacg taatcgttcc ggcggtgtgg cgttgtcaaa agccttaaat gaggcaatga
    18421 gccatatcgg acattgcaaa tcgtgccgta cttttaccga agaggaagag tgtacgattt
    18481 gtaaaaatcc tcgccgtcag atgagcgggc aactttgtgt ggtagaaatg ccggaagaca
    18541 ttcaagcgat tgagcaaacc gggcaatttt cagggcgtta ttttgtgctg atggggcatt
    18601 tgtcgccgat tgatggtata ggccctcgtg aaatcggctt agatttattg caacaacgct
    18661 tggaacagga atctttccac gaagtgattt tggcgactaa tccgaccatt gaaggtgatg
    18721 ctaccgcaaa ttacattgct gaaatgtgca gaatgtataa cgtgaaagct actcgtattg
    18781 ctcacggtgt gccggtcggt ggttcattag aaatggttga tggcacaacg ctttctcact
    18841 cttttgcagg acggcgtgat attgatattt aaagctattt ctgtgctaac ttaattctga
    18901 tataattact caactatttt aaattattat tttgaggcta aaaatggaaa ctattgacaa
    18961 aatcaaacaa caaatcagcg aaaacccgat tctactttat atgaaaggtt caccgaaatt
    19021 cccatcttgt ggtttctccg ctcgtgcggt ggaagcggta attaactgcc aagtgccttt
    19081 cggttatgtg gatattttaa ccaatccgga tattcgtgcg gaattgccaa aatttgcaaa
    19141 ctggccgact ttcccacaat tatgggtaga aggtgaatta gttggcggtt gtgacatcgt
    19201 attagaaatg ctccaaaaag gcgaacttca aaccttatta aaagaaaccg cagcaaaaca
    19261 cgcagcataa tttcaaacca gtatgttaag gctcttatgt ttaagagcct taattttttc
    19321 ttgcgattta ggcttaaact tcttacctca agagctattt tgttttagaa tgtcaaaatt
    19381 atttcgtaac ataaaaataa aaggacacaa catggcatta aaagagttag attgggcaaa
    19441 tttaggcttt tcttatatca aaaccgatta ccgtttcatt gcacattgga aagacggaaa
    19501 atggaatgaa ggcgaattaa ctcaagacaa tacactgcat attcacgaag gctcaactgc
    19561 cctgcactat gggcaacaat gttttgaggg gttaaaagcc tatcgctgta aagatggctc
    19621 aattaactta ttccgcccgg atcaaaatgc cgagcgtatg caacgcaccg cagatcgttt
    19681 attaatgcca cgtgtgccaa ccgagctgtt tattcgtgcc tgtaaaaaag tggtaaaagc
    19741 caatcaagat tggttagggc cttacggttc gggggcaacc ctttatttac gtccgttttt
    19801 aatcggcgtg ggcgaaaata ttggggtgaa agctgcacct gaatttattt tttccgtatt
    19861 ctgctgtccg gtgggggcat atttcaaagg tggcttagca ccctcaaact tcattacaac
    19921 cgactatgac cgtgcagccc caatgggaac aggtggtgtg aaagtaggcg gtaactacgc
    19981 tgcgagttta ttaccgcacg aattggcggt tgagcaaagt tcaactaccc gtaaatttgc
    20041 cgatgcgatt tatttagatc cgaaaaccca taccaaaatt gaagaagtgg gggcggcgaa
    20101 cttctttggg attaccaaag acaacaaatt catcaccccg atttcagagt cgattttgcc
    20161 aagtattacc aaatactcat tgctacacct tgctcaagag cgtttaggta tggaagcgat
    20221 tgagggagat gtgtatatcg accagttaga ccaatttgcc gaagccggtg cttgtggtac
    20281 ggctgcggtg attacgccgg ttggtggtat tcaacataaa gacaaattcc acgtcttcta
    20341 ttcagaaacc gaagtggggc ctgtcacccg ccgtttatac gcagaattaa ccggtattca
    20401 atttggcgat gttgaagcac cgcaaggctg gattgtaaaa gtagagtaat tttacacaag
    20461 taacaagcgg tcgaattggc aaaacttttt gcaaattcaa ccgcttgtct ttatttagag
    20521 aatatgaaaa agttaatttt tttaggttta tcagcacttg cattagctgg ttgttcctca
    20581 acaatttcta atcctaatca actgattggg caatggcaat gcactattga gtacgatgat
    20641 tttaatattc gtacggtgga taatttacga ttttccacaa atgggactat gaccaatcaa
    20701 ggtggtatat attaccctat tcaaaagtcg atttttgagt attcattaca acagaatggg
    20761 cgctggagtg tgaaaaacgg ggctattgtt tatcgtatcg tctcagaatc tgtacagcgt
    20821 agccatcgtt cacaaatatg gtctgaatta caacgtgatg cggagctaca acaatttgaa
    20881 gagaatttat ttagctcact ttctgatagt gataataata aaacagtgga actaatcatc
    20941 actgattttg atgaaaaaga aatggatatc aagcaagaaa taaaagggca taaagcttat
    21001 aaagggcaat gtataaaaca gtagaggtaa aaatgattca acgttttgaa gtaggtagcc
    21061 gtttttcgga aatggcaata cataacggtg tggcttactt ggcagggcaa gtgccgttag
    21121 atgacaccgc cgatgcttac acccaaaccc agcaagtgct tgcagaaatt gataaatggc
    21181 tggcaaaagc caacagcgat aaaagtaaaa ttctaatggc tacggtttat ttaaaagaga
    21241 tggcagatta tgcggaaatg aaccgtgcgt gggacgaatg ggttgcccca aataacgctc
    21301 caccaagagc ggcggtggag gctaaattag ccaatccaaa ttggaaagtc gaaattgtgg
    21361 ttaccgctgc ggtataaatg aaaagcagaa agacgatctc tgtttttcat tatgattcgt
    21421 acattttttc gatttgttgc ttatagcgtt ctagaatgac ctttcgtctt aattttaatg
    21481 taggcgtaat ttcttccatt ttggtgctaa atgcttgcga aagtagtgta aactttttga
    21541 tctgttcaaa acttgcaagg tctttttgta attcgttaat gcgttgttca aacattctga
    21601 caacctcata atgtttaatg agttctaatc tgtcttgata gcgaatattg agcttttttg
    21661 catactcttc gaggctatca aagcaaggaa ctatcaacgc agaaacatat tttttggcat
    21721 cagcaatcac cgcaatttgc tcaataaact tatctttacc tatttttcct tcaatgtatt
    21781 gtggggcaat atatttaccg tttgaagttt tcattaattc tttaatacga tcggtaatat
    21841 aaagattgcc ttccgaatcc agttcaccgg catctcccgt ttttagaaaa ccttcagcag
    21901 taaaggcttg agcagtggct tccggttttt tgtagtaacc tttcataacc aagcctccac
    21961 gcactaaaat ttcattttct tcactaattt tgacctcaac atcaggcatt aaggtaccgg
    22021 ttgagttgat attaaagcct ttttcctgcc agcatgaaat agtggcagta gtttcggtca
    22081 ttccgtatcc aagttttaca ttgatgccta tactttggaa aaatagcccg atagacggat
    22141 ctaatttagc tcctccacac ggcatcatac gaatacgacc gcctagtaag gctcgtaatt
    22201 ttcctagcac caatttatct gccataatgt actgcaagcg gtggaaaaag gctgattttt
    22261 taccaaacag attgtcttcg gcggtacgga ttgcccattt aaataacgct cggcgatgta
    22321 tcggtgcttg ttggacttta tcccacaccg cagtatagat tttttcatag aaccttggta
    22381 cggcacacat taatgtaggt cgtaccgttg agagtgcttg acgtatctgt tgaggatctt
    22441 ccagatagca atttactgcc cctctgtata gcacataagt gacccaagca cgttcaaaga
    22501 tatgcgataa aggtaaaaat gaaagggata tatcattttc gtcaatatga gggagtgctt
    22561 tattatgtgc ctgaagttga tgagctaaat tagcataatc taacataaca cctttaggct
    22621 caccggttgt tcccgaggta taaattaaag tgaataaatc ctctaagcat ttatcattta
    22681 aacgtttggt taattcagct tcatcttgat taaccttaat gaaatcttgc caatgttgag
    22741 cgatagggaa atcacgcaga tctattgagt ctttcatcgc gacaattttt attaatagcg
    22801 aacagttgtt cgctatttct atggcttgat catattgttc ctgatcgcca acaaagagga
    22861 tttttatatc cccgtcattc aggatatatt caagttgttt ggtggtactg gttgcataaa
    22921 tcggcactgc taccgcacga atttgcattg cagcaatatc ggtaattgtc caacgaggca
    22981 tattttgtgc aaaaatagcg attttctctt gaacggcaag accgtttgtc agcaaggcat
    23041 aagaaagatt attgacctgt ttttgtaatt ctaaccaact tatttctgac catttttcat
    23101 cttgcataaa acgtaaagct gggtggtggc taagagcctg aacttgctgg tggaaacggt
    23161 ggataaagtg aaaatctaaa gtgctcataa tcttattttt actttctatt tcagtgaaca
    23221 gttgtacgct aatataatag aaaatatgaa aaaaagaaac agttgtactc tgaaacaaag
    23281 ggttttggat aggaagtaat caaggcaata gaaatagata agtagggtat cgtttagtac
    23341 aaatgcaata tttttcaaaa atgagaccgc ttgtatatgc aacaagcggt ctgttttgtt
    23401 aaaaattttg caactaaatt cgttcaatat caaattgagc taaatcaatc acatcaggtg
    23461 taccgtaaaa agcacagagg gctttacgca aggtttcagc tcgtttggga aggtagccac
    23521 gttctgcata gagcttgact tggtcgtaca ctgcttgttt aaaggctgaa ttatcgccgg
    23581 gattgcccga gagattgtcg gcactagcaa aataacgaaa accggcagca gttgctaaca
    23641 cccattcaat cgcttgcggt ttgatttccg cactttcaaa ttcacgttgg cgttcggcag
    23701 agcgtccgtc cggctcgtac caatagccaa aatcttcaag tttacgccga gcttctccag
    23761 caacgagcca atgggcaatt tcgtgcaagg cactgctgta atagccgtga gcaaataaaa
    23821 tcacattgta agggcgttcg ctcggcacac catcttcgtc cataaattcg ggcaagtaaa
    23881 tcggataatt acccccccgc tctaggcgag tgttatattc attagcaaag cattgattaa
    23941 aaatgcgaat aatgtcttca attttatgat tttcttgatt catttctttt ctttataact
    24001 tattcagcca agctaaaaat tgctcggcat ccaaaaagcc agtaacacga gattggctca
    24061 tttcattgcc gttttcatca aaaaacagaa tcgttggcaa acctaataca ttaaagtgtt
    24121 tcatcagctc gatattttca gccgagtttt tcgtcatatc gatttgcaac actaccattt
    24181 cattcagttt gttctgaaca ttttggtcac taaaggtgta tttttcaaac tctttgcagg
    24241 cgacacacca gtcggcatag agatcgagca tcactttttt acctttggaa gcggtcaatt
    24301 ttgtctctaa atcggcaagt gattgaattt tttctaacgc taagtgcggt gtagtttgct
    24361 gttgagtggt tgagccgttc caagctaaat ctgcccaagg tttagcggca atggctaaag
    24421 cgagcaataa taagactttg atgatctttt tcgtgattga attagatgga atcgcatcca
    24481 ccaaccagat caaaaatgca accgcaaggg ccgaccataa aaacggttca tagtggcttg
    24541 gcagcacacg gcttaataag aacaccggta aggcaagcat aacaaaaccg aatgcagctt
    24601 tcacttttaa cagccattcg ccggattttg gcagaatttt gttgccgaat aaggtaatca
    24661 aaatcagcgg taagcccatt cccagtgcga gtagatagag ggctaagcca ccggtgacta
    24721 aatcgccact ttgtgccaca taaagcaacg caccggaaag tggggcagaa gtacaagggg
    24781 aagcaattaa gcctgcgatc atccccatta aaaacacgct acccaacgca ccaccttgct
    24841 gtttttggct cattgcattg agtttttgtt gccagctatt tggtagctgg atttcgtata
    24901 agccaaacat tgagcaggca agcagaataa agacgataga taaaccaatc aacaccggtg
    24961 ggctttgtaa ggcgatttgg aaaggcaagc cgatagccgc tacaattaag ccaagtaagg
    25021 tgtaagtaac tgccattcct tgcacatagg caatacttaa cagcaatgct ttagcggtag
    25081 aagggcgttg cttatttcca attacaatcg cagagagaag tggtagcatc ggcagtacgc
    25141 aaggagtgaa tgctaatcca acgcctaata caaaaaacca aaaaatggaa aaacgattgt
    25201 tagctaaatt ttcagccagt tgatcttgct ctgcttttgc cacattttca gggggcgcca
    25261 tattgtcggt ttttgcaaaa ttttcgctct tttcgaccgc ttgtgcttcc agttgcgcca
    25321 gttcttcagg tgtagcagag agaatactgt ctaatttaag ctcaacccgt tccggtggat
    25381 agcaaaagcc tttggtacac ccttgatatt ccactgcgat tgaggcgttg tctttaattt
    25441 ctttaaacgg tacctttagc cctaagtggt tgcgatagat ttccacctgc ccgaaaaatt
    25501 catcgttata ttgctcggca gcgggaaaag tgatgtcgcc tatctcaata ttctgtggcg
    25561 tgacacggat ttctttttta taaagataat aattgtctgc aatatcccaa tttaactgta
    25621 atgtttcatt ttcattttgg attgaaaaaa caaaggcttc ttccgatttt aggaatttgg
    25681 gctgagagaa aagatttgcg tgagatgagt ttatcatcaa taaactcaag acgagtgaaa
    25741 aaaagtatct aatcatattg aaatcctatt tttaattgtc tcggcgaaaa ttatagcgta
    25801 atcagtattc atattccgaa taaattacgc catttaacga tgttagacga tgtaagtgct
    25861 attttattac aaaaattttg ttctaaaaga aaaaaaatat tgcatagtta tgcaaaaata
    25921 attagtattc attcattatt ttaaatgtag taggatttta acgaatggca attcgtattc
    25981 aaaatgtcaa ttttttttat ggctcaaacc aagccttatt tgacattaac ttagagattg
    26041 aaaaagggga tactgttgta ttgcttggcc cgagtggggc aggtaaaagt accctaattc
    26101 gaaccttaaa cttaatggaa gtaccgcaat caggcacttt ggcgattgca aatcatcaat
    26161 ttgatttaac tgctaaaacc gacagcaaag cggtgagttt gttacgccgt gaggtgggaa
    26221 tggtgttcca gcagtatcat ttatggccgc atttaaccgt aatgcaaaac ttaattgaag
    26281 ccccgatgaa agtgcttggg ttaagcaaag aagaagcggt tgccagagca aaaggacatt
    26341 tggcacgttt gcgtttgagc gagtttgccg agcgtttccc actgcatctt tcaggtggtc
    26401 agcaacaacg ggtggcgatt gccagagcac tgatgatgca gccacaggtc ttattatttg
    26461 atgaacctac cgcagcactt gacccggaaa ttactgcaca agtagttgat attattaaag
    26521 aattgcaatc tacaggcatt actcaggtga ttgtgactca cgaagtaggc gttgctcgta
    26581 aggtggcaag caaagtggtg tacatggaaa aaggtacaat tattgaacaa ggtgatgcaa
    26641 gttgttttga atcaccgaaa acagagcaat ttgctcaata tttatctcac tcagagtaat
    26701 ctataacaac agaaaaggac acaacaatga aaaaattatt attagcttct gctatcgcat
    26761 ttgcaacgct ttctgccaat gcacaagaaa ctatcacttt tgcgatggag ccttcttacg
    26821 ccccttttga aacaacaaat gaaaaaggtg aagttattgg ctttgatgta gatattgcaa
    26881 aagcattatg tgaagaacta aaagcaactt gcgaatttaa aacgcaatca ttcgatagct
    26941 taattgcggg attaaaattt aaaaattttg atgcaattat ttcaggcatg gatattactg
    27001 aagtgcgtca aaaacaggtt gcctttacca atgcttattt agaaaactca gtaagttatg
    27061 tcgcaatgaa ggggaaattt acaccggaaa cagctaagaa tgtcggcgtg caaaatggta
    27121 caacttttca acaatactta agcaaaaatg ccaaacaata taatttaaaa ccttacgcta
    27181 gcctacaatc agcagtgtta gatttaaaaa atggacgtgt tgatatggta tttggtgata
    27241 caccagtatt ggcagagtgg ctaaaaaccg aaaaagagtt agatttcgtg ggtgaaaaag
    27301 tagtcgataa agcctttttt ggtaatggat atggtattgc ggtaaataaa tctaacactg
    27361 cattgcttga aaaattaaat tcggctttag ctaccatcaa aacaaatggt aaatatgagc
    27421 aaatttataa tacttggatg gctgggaaat aatgtctagc gatattttaa acttacttgt
    27481 caacgcaacc ctgatgacct tagggattgc gctggcaagc ctcttgattg gaatgttatt
    27541 gtcaatcttt tttgtggtac ttgaaaccag taagattgca tgtatccgta aaccaactag
    27601 tataataatt acattattgc gtgggttgcc ggagatttta gtggtatttt tgatctattt
    27661 tggtcttatc caagttattt tcttgatcac cgatgagttt gcggagttaa atgctttctg
    27721 gtgtggggct attgcgctgg gtattatttt tgctgcttat gcttcccaat ctttacgtgg
    27781 tgcaattaaa gctattccag ttgggcagtg ggaatctggt acggcgttag ggttaagccg
    27841 agcgcaagta tttgttcgca ttattctccc tcaagtctgg cgacacgctt taccaggctt
    27901 aagcaatcag tggttagtgc tgttaaaaga tacagcattg gtatcgttaa ttggggtaga
    27961 tgatttaatg cgtcaatcgg agttaattaa tgctaatact catcaacctt ttacttggta
    28021 tgggatagca gcacttattt atttagcgat tactctaatt agccagcgag tgattaaagt
    28081 attagaaggt agatttaccc gttttgagcg aggagttgct taatgtggtg ggattattta
    28141 gttgaaattt cacagggtat tggcaccagc ctacagctca cttttgtggc gctcatcatt
    28201 gcctttattt tagcgatcat tcttacgttt attctctcgt tagaaaatcg tccgttaaag
    28261 tggttggtaa acggatttat tacgcttttt accggaacgc ctttattaat tcaatttttc
    28321 ttgatttata gtggcccggg gcaatttgaa tggattacag aaagtgcggc ttgggtatta
    28381 ttatccgacg cttggttctg tgcaatgttg gctttagcgt taaacagtgc tgcctattcg
    28441 acattgttgt tccacggtgc ggtaaaagcg attccgaagg ggcaatggga aacttgcgca
    28501 gcccttggtt tgagccgttt agatacactg aaaattttag tgccttacgc attaaaacgt
    28561 gctttacctt cttatagtaa tgagattgtg ctagttttta aaggcacatc gcttgcttca
    28621 accattacga ttatggatat tatgggctat gctcgccagc tttatggtac agagtatgac
    28681 gcattatcaa tttatggtat ggcaggggtt atttacttgg taattaccgg cattatgaca
    28741 atcgttctca gaaaattaga aagcagagta ttagcttttg agcgttttga gactgaaaaa
    28801 gcctagcgga taagcggtca aattttattg attttttgca aatgaaagcg gatagtacta
    28861 actatccgct ttttatttat gtatcaatgc ctttagaaat atgactaatc tatttgggtt
    28921 atacctaaat gggcataggt tctgctggta gcaattctgc ctcttggtgt gcgttgtaga
    28981 aagccttgct gaatcagata aggctctagc acatcttcaa tcgtatctcg ttcttcgccg
    29041 atagctgccg caagattgtc taatccaacc ggcccgccat caaagcgttc tacaatcgct
    29101 tgcaacagtt taatgtccat aaaatcaaag ccttccgaat ccacatcgag cattgccaga
    29161 gcttgtttgg caatgtcgga ggtaataatg ccattattac gcacatcggc gtaatcccga
    29221 actcggcgga gtaagcggtt ggcaatacgg ggtgtgccac gggagcggcg agccacttca
    29281 taagcaccgt ctgccgacaa attgagattt aagcaatcgg cgctgcgttt gacaatcaga
    29341 gtaagatcgt ccactgagta aaattctaag cgttgtacaa tcccaaaacg atctcgtaac
    29401 ggtgaggtca gtgagccggc acgggtggtt gcccctacaa gggtaaaagg cggtagatcg
    29461 agcttgatgg aacgagcagc aggtccttcc ccaatcataa tatcgagctg gtaatcttcc
    29521 attgcaggat aaagcacttc ttcaatcgct ggagaaaggc ggtgaatctc atcaataaac
    29581 agcacatcgt aaggttcaag gttggtcagc attgctgcca aatcgcctgc tttttccagc
    29641 accggccctg aagttgtgcg aatattcacc cccatttcgt ttgccacaat gttggcaagg
    29701 gtagttttcc ccaaaccggg cgggccgaaa atgagcaagt gatctaatgc ctcttcccgc
    29761 aatttagcgg ctttgatgaa gatctccatt tgatcacgca cagaaggctg cccgacatag
    29821 tcggcaagca gtttcgggcg gattgctcgg tctataactt cttcctcacg cttaggcgag
    29881 gcactgatga ttctatctgc ttcaatcata catttagatt attgttgtag tggaatggta
    29941 ttaccctata ttactcatga gggcaaatat tgatttgccc ctacacatat atttttaaaa
    30001 atgagattgt aggggcgaaa tatctttcgc cctatactat caatatcata atgaattttt
    30061 taacgcctca cgaataagct gttcgctcgt tgcaccggat ttattcactt tcttaatcat
    30121 tttttccgca tcggcaggtt tgtagcctag ggctattaag gcatcaaggg cttcatttgc
    30181 cgggtctggc gtattacttg ccacaatttg ctcgtgcgat acttccacaa agaaatcact
    30241 ctgtgccatt cttttaaatt tgcctttgag ttccaccaat aagcgttctg cggttttccg
    30301 accgattccc ggaattttgg ttaattttgc cagctcttca ttttcaatcg cggtggcaaa
    30361 ttgcgacacc gacattgctg atagaattgc caacgcgagc ttcggcccga caccgttggt
    30421 tttaattaat tcacggaata gagttctatc ttgtttttgg gcaaagccga atagcaagtg
    30481 agcatcttct cgcaccacta aatgggtgaa aatagtcgcc tcttcgccaa tttgtggcag
    30541 gctgtagaaa ctggtcatcg ggagcagtag ttcatagcct accccttgca cgtctaataa
    30601 aatttcaggc ggttgctttt caataatttt gccgtgtaat cgtccaatca ttttgttctc
    30661 tcagagttaa aattggcagt agcatagcag aaactgtata aaataacact atttattgat
    30721 agcggaattt gcaaattttt agtgaaatca gaccgcttgt gattagataa agtggctgaa
    30781 tccttcataa atcagcatcg agccgaataa gatgaaaatt acgcctgaga tattgtcaat
    30841 atagcgacta tatttggcat agaacagtct gaccggtcgg cgagaaaaga gtagtgccac
    30901 cacacagaaa taaataaagg tacttaccac aaagagtaga agtagttcga acatttgtag
    30961 ggtattgctt aaattatcta cataggctga aatgacgctg gtaaagtaaa cgccagcttt
    31021 ggcattaaag atattgatta ataagccttt tttaatttcc tgccaagggt ttagcggcgt
    31081 gagttgagct ttattttcaa caaaaatgat attttcggtt acccgtacca tttgagagcc
    31141 aatataaacc aggtaacagc caccgataat cataatggca tattgaaaca gcgagccaat
    31201 actattactg agccacgcta aaccaaaaat cgcaaaggct gcccaaaaaa taatgccgat
    31261 tgagattccg gttgcggcaa gtaccccact tttaactgag tggctggcgg attgtctgac
    31321 gacatagaag aaatcggggc cggggctgat aagcccggca aaatgaataa ataaaatagt
    31381 gagcataaat ttttaaatga aataatataa aagatgaagt gaaatcaggg caaaaatggc
    31441 tgcgagaata tcatcaaaca taatgcctaa accgctttct aatttttcat caaaatagcg
    31501 gattggaaaa ggcttcaaaa tatcaaaaat gcgaaagctg ataaaggcga gcaggtagcc
    31561 aagaagattg tattcaggca aacaggtgaa catcaaaaag atggcaacta tttcgtccca
    31621 aacaattcgc ccgtcatcgt gtacttctag atcttggctg gctttggtgc aaataacaca
    31681 accggcaata aaagaaacta gggtgagtaa gccgaaaaac agggcggatt gtgtgataga
    31741 ccacaaggct attgctacaa ttagcccggc gaggctaccc caagtgccgg gagcaggctt
    31801 taataagcct gaaccaaagc caaaggcaaa aaaatgaatc ggatttttaa gattaattgg
    31861 tttcattgcg ttatttttta aaatgatcgt agcctcgttg tgagggcaag gtgacaggct
    31921 gattatgacg taatagggtt aaaccttctt tagccgaacg aatttgccca atacatgtat
    31981 aagttactcc gatattggct aatgctttgt ctaatttggc tttgttgtca tttggcacgg
    32041 taaagcagag ttcgtaatct tcgccaccgt ttaaggcaaa atattctgcc tctgttcgag
    32101 aataacaact aagtaagacg tcagacaagg ggaaattctc aaggtttaat actgcaccac
    32161 agtcactgcg ctttaaaata tggcttaaat cagacaatag accatctgaa atatcaatcg
    32221 ctgcattagc aatagaggcg atagtcaagc caagtaaaac tcgaggtgtt gggcgtaaat
    32281 ggcgttgaat caggtagctt tgctctttat tccaattcac ttgctcgctt tgctgattta
    32341 gcagaagttg caaaccacct gcactgtcac cgagtgtgcc ggaaacatag atccaatcgc
    32401 ctactttagc attatggcgg aataagcctc tatcttgtgg cacaatccct tgagctgtga
    32461 ttgtcaaaga tagtggtcca caagtagtat cccctccaat caaatcaaca ttatagtgat
    32521 caagaatctc aaagaaatgt tcactaaatt cagctagcca atcgtgatta atttcaggta
    32581 aggttaaggc taaagagatc catgcaggtt ctgcacccat tgcggcaaga tcgcttaaat
    32641 tggacgccac acttttataa gcgagatcgg caggtgaaat agtcggtaga aaatgagtgc
    32701 cgcaaacaag ggtatcggtt gtgatcgcaa gttgctgatt tggtttgagt gatgtgagcg
    32761 cacagtcatc gccgatagaa agtaaaacat ctttgcgagg tgggcgttga gaagcattaa
    32821 aatagcgttc gataatatca aattcaccca taaaaaatct ccttgagcgt tataatcaaa
    32881 atccccaatt tatgtataaa ttggggaatc ttattatttt ctgcctaatg ctggggctaa
    32941 tttatctaaa atgccgttaa tgtatttgtg gctgtcatca gagccgaaca ctttagcgac
    33001 ttcaatccct tcattgatga ctacacggaa aggcacatcg tgttcaaatt ttaattcgta
    33061 agttgataaa cgcaaaatag aacgttcaat aggatcaaca tctgactctt tacgatctaa
    33121 ataagggcgt aatgcttcat caatggcttc tacattatcc acaacgccac ggaaaagttt
    33181 acggaaataa ggcatatcaa cgcctttcat atcttgatcg gtcacaaacg ccaattccac
    33241 ttcttcaacc gtgttttggg aaatatacca agaataaatg gcttgcaccg cacattctct
    33301 tgcacggcga cgtggggaaa ttttcataaa acctctaaat ctttaactca aaaaatgacg
    33361 gctgattcta tcacacttcc cctatttttg cttgcgttta tgatgtgcta ttttgatttt
    33421 ttgtggttgc tgctcttttc attagattga aggatataag gcgtcacaaa aatgactagc
    33481 tctcttttgc tgattttatc tcgagtttga ctaaataacc gcttgatgaa gggaattgag
    33541 cctaaaatcg gcactttatc ttcgcctttt tgggttaaat gttgaaaaat accaccaagt
    33601 acaatggttt cgccgtgttt ggcaaatact tgcgtattta gctcttgttt atcaatcgta
    33661 attaagccac tactgccgga ttgggaattt ggcgaatttt gggtaacgat cagatcgagt
    33721 agaatttgat tttgggttga aaggtgtgga acgacttcaa gccctaatac cgcttcttta
    33781 aattcgacat cggtggcttc cgatttggtg ttgtacatca cataaggaat ttccgtgcct
    33841 tgcttgatac ttgccggctt tttattggtg gttaacaggc gtgggctggc aataatttcc
    33901 acactatttt cttgttctaa tgcacttaat tctaaatcaa gcactctgct attaatggac
    33961 gcaacctgta atacggcaga tgctccacca gtaatcggaa aatttacatt taagttattg
    34021 gtgacatcgt tgagtccatt gccttccagc cgtccgccaa atttatagtg cccagcccca
    34081 cgagaaaaaa ttccccagcg aacgccaagc tcttgcaaat gctcgctact gatagtgaca
    34141 attctagcct caatcgcaat ttgctcggtg ggttggtcga gctgttttac taaattctcg
    34201 atatttttaa gcgattttgc actgtctttc acaatcaggc tattgcttct ttcatcaaag
    34261 tgaatataac cgttttccga taaaaacgtt ccgctgcctt tggttaacga ctcaatcacc
    34321 tctgaagcct ttgcataatg tagtttcaag gtttttgtga ttaacttagg ggcggtgaca
    34381 agcggtgtat tttgcccatt attttgcaaa ttcattattg gcagaggcgt taagtcagcc
    34441 gctttttcct cttttttgtg aatataaaca atatcatctt cttgcgttaa ggataattta
    34501 ttgactttgc caagcgtttt aaaaatctcg tcaaccgatt tattttctaa tcgtagtgtt
    34561 gatttggttt caatattatc atcaagcacc atatttttac cggtttcttc cgctaaataa
    34621 gtaaaaataa gcgaagttgg agcatttttt aaaactatgg aaatggtatt cgcataggct
    34681 aatgaaaaac cgcataagaa atatataaaa attcgccaca tatcaacctc tataatttga
    34741 tgttaatttc ataagggata ttacaatctt tagttaattt ccagttaata taagtaatgg
    34801 atttaagatt aatatgtttt atttcaattt cattgttaag caagtcattt tcttttagag
    34861 caagtaattg ttgtttttca tcaataaata atgcacgcat tacttcattg attttaacta
    34921 atcctatcag tgtcattttg tcaaaatcag ttactatatt ttttgcagaa aggttatcag
    34981 gtactttaca agcggtgatg ttttcagtat tttttgcaaa ttccacattg ctttcagcga
    35041 tgttgcctga ggcttgagtt tcctgttttt ctccaaagaa aggatcattt gcttttacag
    35101 aaaaactgat aaataaagta ataaataaaa accaattacg cattgttatt ccttattagc
    35161 taatatgact aaggtagtat tcatttcaac ttttctgtct tgccgagatt tgatgaggct
    35221 aatcgcattg tatttcaaat aaggtatttg cttaactgaa tggataaaat caaataaaga
    35281 tcggctattg ccgataatac tcaattcaat acttttacct tgttctaaat tccattgtaa
    35341 actttcaatg gttagttggt gtttttcagc taatatttgt atgttgctgt ttatttcggt
    35401 taaattattc tcttggctgt tctgattttt agctttattg ataattgagt catatttgtt
    35461 ttgttgatga ttaaagttaa tc
//
LOCUS       DANKBV010000008        34812 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.008, whole genome shotgun sequence.
ACCESSION   DANKBV010000008 DANKBV010000000
VERSION     DANKBV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 34812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34812
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.008"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..393
                     /locus_tag="PWY43_000302"
     CDS             <1..393
                     /locus_tag="PWY43_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2135218.1"
                     /translation="KRTLNQPAGHLEKDETLLEGASRELFEETGIRAEMANLVKIYQW
                     QAPRSQTDFLRFLFSVELEEWLEPNPQDADITQALWLTLAEFEDYIQQQGQCARSPLV
                     VQSVKDYLSGERYPLSLLNAFEWARNGK"
     gene            383..1591
                     /gene="ubiH"
                     /locus_tag="PWY43_000303"
                     /gene_synonym="visB"
     CDS             383..1591
                     /gene="ubiH"
                     /locus_tag="PWY43_000303"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDL2135219.1"
                     /translation="MENKTTTQFDVVIVGGAVTGSVLALALSSFTHHKISIAIVEKSL
                     PDYEQQGGFDARSIALAEGSLQKMGQIRPLAGSSLGEMISEIGTPIQQIQVADKGHFG
                     KTTLKASEQNVAQLGMVVELAKFGRKLTACLAQQANITLFCPNQVVGIERSQQHCGLS
                     LKNGEKLSCSLLVAADGIQSQIAKQCGVETVKVRDYAQSAIIANVELSESHQNQAFEY
                     FTPQGPFALLPLSGNMMSLVWCVKEPNELIQLSDSEFLARLQQQFGWKLGQFVRVSKR
                     FVYPLILQKAQSHIHHRLAVVGNAAQMLHPVAGQGFNLGLRDLTTLAELVALAFNQGK
                     DIGEFSLLSTFENERTQDQARMINATSGLISLFCCEMLPVQVVRNLGLLTVSHSKIAR
                     DFVANQALGW"
     gene            1854..3086
                     /locus_tag="PWY43_000304"
     CDS             1854..3086
                     /locus_tag="PWY43_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HDL2135220.1"
                     /translation="MTTENMSTPKQAFIGLQMLFVAFGALVLVPLITGLDPNVALLTA
                     GVGTLIFQICTKRQVPVFLASSFAFIAPIQFGVQQWGIPVTMGALLCSGFIYMALSAL
                     VKAKGVNILHKYFPPIVIGPVIMVIGLGLAPVAVDMATGKSANIAYNTAIIVSMITLV
                     TTLIVAVFAKGLLKLVPILAGLTVGYILSVFMGLVDFTKVVEAPWFSLPKVSAPEFKL
                     DAILYLLPIALAPAVEHLGDVMAISQVAGKDFMKKPGLHRTLLGDGLATSAAAALGGP
                     PNTTYSEVTGAVMLTKNFNPKIMTWAACWAIAFSFCGKIGAFLATIPTFVMGGIMMLL
                     FGAVAVVGINTLIKAKVDLSIPRNLCIASVVMTFGIGGMLINIGEFSLKGISLCALVA
                     IVLNIVLPKEKVEDSAAH"
     gene            3153..4139
                     /locus_tag="PWY43_000305"
     CDS             3153..4139
                     /locus_tag="PWY43_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2135221.1"
                     /translation="MQKLTFSPPLLNTPCPWCSELESLRELYACEYTGAVTTRTSMPQ
                     PYPHDWAKNQYVLFDSNAQKTASVNTQDATSLQTASLNTIGLSPNNLDTTISFVRTIS
                     NELTAPSSKAFIISVFGSPEEVGECYEKIVAFQTEVKMPLAMEINISCPNIPGEISPA
                     YSAEELSHYLHALQTSLKKLAQGDNALPIGIKIPPFTYQNQYDELIKGLLQAVENGEN
                     LPISFITSTNTLGSSLVLNAGKAVLNSEVGTGVGGMAGTAIHALSLGNVYTIRQMLDQ
                     EPKLKHLQIIGVGGVNDKAGFERMKSVGADFVGLATALGVEGIGVFKEILSE"
     gene            complement(4180..4590)
                     /locus_tag="PWY43_000306"
     CDS             complement(4180..4590)
                     /locus_tag="PWY43_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HDL2135222.1"
                     /translation="MLYSVLTAHILLNEPYRNGAHSTQHEHLVVMFEIDEESGYFYAM
                     DLHQTEQPVVDSLFVFRVSDIEQNSLKEPRRLEICWSEDGYQAFLLINGYPHAVFDFR
                     QFVGYNHTKFPMPELGSMWVHKETTNELVEKWLG"
     gene            complement(4656..5966)
                     /gene="eno"
                     /locus_tag="PWY43_000307"
     CDS             complement(4656..5966)
                     /gene="eno"
                     /locus_tag="PWY43_000307"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL2135223.1"
                     /translation="MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTG
                     SREALELRDGDKGRFLGKGVLKAVSAVNNEIANALVGKDGSAQAEIDQIMIDLDGTEN
                     KSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
                     DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKAKGLNTAVGDEGGFAPNLAS
                     NADALACIKEAVEKAGYVLGKDVTLAMDCASSEFYDKERNVYDMKGEGKTFTSQEFTH
                     YLEGLCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDLFVTNTKILKNGIEK
                     GIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQ
                     IKTGSMSRSDRVAKYNQLIRIEEALERAGTPAPFNGRKEIKGQA"
     gene            complement(6381..7100)
                     /locus_tag="PWY43_000308"
     CDS             complement(6381..7100)
                     /locus_tag="PWY43_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2135224.1"
                     /translation="MSKPNIYDESFSERMKWIAKNSFKDNYSEFARAVGVAQASLSRW
                     VKGEADPSRSNLVKIAEVSNVSLEWLATGKESKPQSENTSIVAREFERLEKMADDIVS
                     MINGFSTINVSAGFGSFNEGVTKPDGQEPYADSLLQSLGVKAHNCGVFWANGTSMHPT
                     IADGDQMLVDFSKREPRGDDKIYLVQNGESVWVKRIRKEWDYIELISDNESYRPIQIT
                     AEEAQNLQIIGQVVHIGHSLV"
     gene            7277..7555
                     /locus_tag="PWY43_000309"
     CDS             7277..7555
                     /locus_tag="PWY43_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2135225.1"
                     /translation="MSVLSDVKKTAQRDWHRADILAELRKKGWSLRTLADEGNVSYNT
                     LKSVLDKPYPKMERLVANAIGVAPEVIWAGRTAERIERNRRPTLTNKF"
     gene            7599..9434
                     /locus_tag="PWY43_000310"
                     /pseudo
     CDS             7599..9434
                     /locus_tag="PWY43_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9482..10363
                     /locus_tag="PWY43_000311"
     CDS             9482..10363
                     /locus_tag="PWY43_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2135226.1"
                     /translation="MKNQELRALMDSKGYQQKQVAQLLGVSVATVSLYLKGDYNGNVA
                     EMDRKVDELIERDKAKVVEAKYNAAFVPTLAARRGMEVMQFAHIEGEINVIFGAAGLG
                     KTQMLKEYERRNSSATLIEVDPSCTPKVLLRKIAEAVGANARGVNNELLESIVNKLKG
                     SERLLMIDEAELLSTRSLEFIRRIHDLTGVGVVLAGMPRLLVNLKGKNNELAQLYSRV
                     GFACDLGNALSEEDLGLLAESALGTNEFNAPLIKACKGNARRLSKLMRGVVRSSEINQ
                     TPISKDLVEQYSKMLIS"
     gene            10374..10622
                     /locus_tag="PWY43_000312"
     CDS             10374..10622
                     /locus_tag="PWY43_000312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135227.1"
                     /translation="MTCFRHWTQRVTDGTEDLFWLDELLVGKDERDGSFTLSIFDPDI
                     DDFLLYEKVKTRKDWEIAYRKAREIINEQDIDCRSFRV"
     gene            10632..10952
                     /locus_tag="PWY43_000313"
     CDS             10632..10952
                     /locus_tag="PWY43_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135228.1"
                     /translation="MQKVFSEKAGEMMNPFNAYIHDSLVKLEIATLECEALDLEIERV
                     EFEAASPPRLVLRDNRKTQDFVKNGLASLFGMKTKGGRRYDSYQMQVRGVKCVWESER
                     VTKV"
     gene            10955..11146
                     /locus_tag="PWY43_000314"
     CDS             10955..11146
                     /locus_tag="PWY43_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135229.1"
                     /translation="MKKRRMIYALYVGEENVADGTAEELAKRLGVEAKTIRFMSSPAH
                     NKRVKYNGKAVRTVKVGLE"
     gene            11159..11776
                     /locus_tag="PWY43_000315"
     CDS             11159..11776
                     /locus_tag="PWY43_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HDL2135230.1"
                     /translation="MSKVTIGNEIYWKDADGNLKPEALVKEIDKERDELVRQFVSKAQ
                     ALSKALGDFKQSVFDDVGAFVSLSAEKYGVKIGGAKGNITLFTYDGEYKLQLAVQENI
                     RFDERIHAAKALIDECLHDWSEGAKPELKALIDNAFEVDKEGNLSTAKILSLRRVEID
                     DARWNQAMTAISDSVQVIGSKGYIRFYKRDENGKYQPISLDMAGV"
     gene            12095..12307
                     /locus_tag="PWY43_000316"
     CDS             12095..12307
                     /locus_tag="PWY43_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL2135231.1"
                     /translation="MKTEQKATKFDRFRYYAEKAAEAERKGNYIEAQDHWEVAKLSAK
                     STANLGWAEQRAEFCKRMHNKPFEGE"
     gene            12313..12495
                     /locus_tag="PWY43_000317"
     CDS             12313..12495
                     /locus_tag="PWY43_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135232.1"
                     /translation="MTEQDKNELNSQLNEALMQIIQAQKYLKQSDFIRSGVYLGTVQD
                     LLPKVHLKLLTANRKH"
     gene            12518..13093
                     /locus_tag="PWY43_000318"
     CDS             12518..13093
                     /locus_tag="PWY43_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HDL2135233.1"
                     /translation="MKFRFFKGDITQEPLKSIYADYQVSKEERKDKLNAILEKYPFND
                     GLISRSGWFHRIVIGIACKATNISQVLGVKGFKVSKFDDEFYLVKPDKRYKKGKELAE
                     DFKAINEIYEQHRDFSPYILKRLNMFYFASDFSYSPTSGRSYLAVADIFENTLLVKVP
                     MPIDKNDQPFPDIAEGLVEIKESEFLAIQGK"
     gene            13106..13474
                     /locus_tag="PWY43_000319"
     CDS             13106..13474
                     /locus_tag="PWY43_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135234.1"
                     /translation="MQQQIIKWLANGETGTSSKTMAFVIGFDVIPSRSGYPHDVSDFR
                     LCFQLVEAAPEMRNHIYKMKAISPIWSEFAENWEELERRYRAEQGQGRLPHTYALIQQ
                     ITDKDQNVIRMGNVSFSFGQ"
     gene            13546..13716
                     /locus_tag="PWY43_000320"
     CDS             13546..13716
                     /locus_tag="PWY43_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135235.1"
                     /translation="MEYQKMPEYRLCKKKSGDIVLQQLVICEITPATWHNPYPDSRTY
                     WVDLETIEEGAE"
     gene            13716..14270
                     /locus_tag="PWY43_000321"
     CDS             13716..14270
                     /locus_tag="PWY43_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135236.1"
                     /translation="MKLCKCPICHSEISLEALIEDDAGRELLNTIVNIGGNCGRAVVA
                     YLGLFKPQKNSLANSRALKIVQSLLELYTPSNVLEKALMDTVEQVRRNRRETGRCEPL
                     ANHNYLKKVYESVKPQFAVVRSEAKAVSSLEQQQRQADQKKHDAVMYVERMKQMGQLE
                     AVKNTESYQIWLKWQEEKQNATAN"
     gene            14254..14676
                     /locus_tag="PWY43_000322"
     CDS             14254..14676
                     /locus_tag="PWY43_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDL2135237.1"
                     /translation="MQPQTRKQMIQKIHIGKNELKLDNNAYKMLLLEAVDKPSCSMMT
                     DSELMTVLQTMKAKGFVVKSKKYGKRPTASKTAPHRQGLINKIGALLYQSQKPWDYAH
                     AIAKRSFGIERVQWLTDEQLHKLVQMLASYNYRHGMSV"
     gene            15032..15631
                     /locus_tag="PWY43_000323"
     CDS             15032..15631
                     /locus_tag="PWY43_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HDL2135238.1"
                     /translation="MNNFQIATFTGSLQNQSTLLINARDLHQMLQIGRDFSNWIKSRI
                     ESYGFVENEDFTCSPNLASEKSKGLASFWGSQNKIDYHITLDMAKELCMLERSELGQQ
                     ARRYFIQKEKEANALKQAQPKATIEISTDKYIELLETQNQLLKQAMKPQKYKLRLSAE
                     EKSHIFRLHQQGLSTGQIVQQSGRSESAVRAVIRENLGK"
     gene            15642..15848
                     /locus_tag="PWY43_000324"
     CDS             15642..15848
                     /locus_tag="PWY43_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135239.1"
                     /translation="MRTEFEDIVQNTENLASGLYSLSHLLEILDNTEDQISMSGLAHL
                     VAIIASATKKQANDAATALNCVDN"
     gene            15941..16831
                     /locus_tag="PWY43_000325"
     CDS             15941..16831
                     /locus_tag="PWY43_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135240.1"
                     /translation="MFSFIKKLFDNKPSSIQNIEEPPKEIESELTSYDETIKTLFEKF
                     LVNYSIPERHNYNVRKRVSKEIIFSQLLESDSFSMPEDKRDRLYNALVISESVDYQEL
                     HAIFLLDDFKWRLFDNCREFCEENKIYPYAYQFLANKPAIPDNLESALLFVKVSQLRE
                     LLKDKLSQKIPTRKDEVYPLFAQHFTLEEMKEILEERFIEVKNQFENTVKNHKINILG
                     QWFTYYSNNLSDYYLYRILDRTLTHPKLDVPEFIRHSYKDLEFIKKAGLSCFDEKGNL
                     TSVPPLFPGDYSGINYGNYY"
     gene            16959..17390
                     /locus_tag="PWY43_000326"
     CDS             16959..17390
                     /locus_tag="PWY43_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HDL2135241.1"
                     /translation="MSEFESVEHYLPESVKEIVDLIGLSATEKLIKAFGGFSFQFSKG
                     KVYFEKLKDVLGEEDAEKLYDYLRSNIAYLPRCQTALRLLRNERIYTDYCRMTEQEGI
                     SGRLAIMQICSKYGVSDRIAWDAVRYLKSKPKQPITQTSLF"
     gene            17476..18009
                     /locus_tag="PWY43_000327"
     CDS             17476..18009
                     /locus_tag="PWY43_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL2135242.1"
                     /translation="MTEPIKNKATIADFNLAFDRLIGHEGGYVNDPKDPGGETNWGIT
                     IHTARANGYTGSMRAMSRADAKTIYLKAFWQRYNCEQFPPELAFQFLDACVNHGSGNA
                     SRMLQRAVGVADDGIIGKVTLAAIERYSVADVGLLFQAERLEFYTKLKHFDRFGRGWI
                     NRMAGNLRYFAKDTVEE"
     gene            18012..18254
                     /locus_tag="PWY43_000328"
     CDS             18012..18254
                     /locus_tag="PWY43_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL2135243.1"
                     /translation="MKQFLLDLITNKDGTASTTGFIQFISWLVLSGILIHAYLYDKPF
                     ISDWWFAYAGICVLGSPATKGVVSALKKDRTKGDEE"
     gene            18251..18610
                     /locus_tag="PWY43_000329"
     CDS             18251..18610
                     /locus_tag="PWY43_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL2135244.1"
                     /translation="MITQQIVGFIGVIGTVIAYAFFKGWQLKQERKLTATLKAEKQQQ
                     AVEIQQKNAEVKNAQIQQKHINETRRIQPSAVDERLHQHGYFRDDYGLHGVRSDLSEP
                     CGHNGNETPSACTQSDL"
     gene            18625..18780
                     /locus_tag="PWY43_000330"
     CDS             18625..18780
                     /locus_tag="PWY43_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135245.1"
                     /translation="MKLIKTLRQRWQQWRFYRRRPDLAELHNVVRAAAAQGNQHPVGR
                     AKRGRYD"
     gene            18773..19030
                     /locus_tag="PWY43_000331"
     CDS             18773..19030
                     /locus_tag="PWY43_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135246.1"
                     /translation="MIDEKLFQMLISFVVAPLVAFVGKVIFDRINRNEQTVKELKAEM
                     ESKYQSKELAHEVNRGIKEKLDDILDSLKEVNQKLDKKADK"
     gene            19030..19284
                     /locus_tag="PWY43_000332"
     CDS             19030..19284
                     /locus_tag="PWY43_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135247.1"
                     /translation="MSRKKQKQTDSEKLDLILGGLNELNDKVDKQNEEIERLHREIQA
                     TNRLVNEMARKNRRDAVVAGGLGGGIVAVGFELLRLKFGM"
     gene            19292..19792
                     /locus_tag="PWY43_000333"
     CDS             19292..19792
                     /locus_tag="PWY43_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HDL2135248.1"
                     /translation="MAHDVEVQKAVRQAYVFDRLSLEMAAERAGVSFGTARRWKANAE
                     KNGDNWEKARDVQAMASGGIENIAQGLLAGFLIKYRTLMTELEENTKMTTEAKVEALA
                     ALADSFAKMTASSKKLLPETSAMATAMRAIEMMANIVKTKKPHLLPDFLEMLDDLEVQ
                     FKKEFK"
     gene            19792..19938
                     /locus_tag="PWY43_000334"
     CDS             19792..19938
                     /locus_tag="PWY43_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135249.1"
                     /translation="MAKKKQTVVHKHYSFRWIVQLACILGAIKLMLNGTDGWGWLLLI
                     AVCL"
     gene            19942..21567
                     /gene="terL"
                     /locus_tag="PWY43_000335"
     CDS             19942..21567
                     /gene="terL"
                     /locus_tag="PWY43_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL2135250.1"
                     /translation="MKIKEFEKELEELRRQLQQNIEASFEGWDDKPQAISERRQKVLD
                     PVHGFEYFVQAYFPHYVRSEHKSQLHHYLFENLPLSVSDLSKSVRQAIAAPRGEAKST
                     ICTQLFPLWCLVRDLKRYIIIAMDTKEQAYSMLEAIKVEIESNPRLRIDFPEVAQGKV
                     WRAGVIMTAKKQKVEAVGAGQKLRGRRHGAYRPDLVVLDDIENDESVEKPAQRNKLHN
                     WVLKAVLKLGAAGEKFDVIYVGTILHYDSVLNRVLSTKGWKKAHFKAILRFPDNMALW
                     DEWENIYLSEEGDDDTLSDLFYQQHKAEMDKGAVVSWLARPILYLMKIRASDGHSSFD
                     SEYQNDPVSGDDAIFANSIQYWTELPENLIYFGALDPSMGKAGASRDPSAILVGGYHR
                     ESGKLYVVEAQIKKRLPDLIIEDVIRLHSQYNCHRWFVETVQFQEFLQTELVKRSAQR
                     GKPVPATATKPNSDKMLRIESLQPHIANGLILLHRSQSTLESQLRHFPKADHDDGPDA
                     LEMLWRNALSSSAPIEWESVDDFDGEYRSKWRH"
     gene            21636..23309
                     /locus_tag="PWY43_000336"
     CDS             21636..23309
                     /locus_tag="PWY43_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HDL2135251.1"
                     /translation="MSKKKHNKQIKPNLNPIKNQELQTDLAEITATGRVLSDHPSNFI
                     TPAKMKAIFEDAENGDITAQHELFMDIEERDSTIFANIQTRKRAALGVDWAIVAPRNA
                     TPQEEKLRDEVDELFYQLGNLEDFVMDCMDAVGHGFAALEIEWAFNGKLWYPNAFIHR
                     PQSWFKWDKVDNLLLKTRQNTEGEPLREFGWVVHTHKSRSTQAARNNLFRSLAWLYMF
                     KHYSIHDFAEFLELYGMPIRIGKYGAGATPNEKQTLKRALAEIGHNAAGIMPESMSIE
                     LHNAANAGGAAGNNPFLQMVDWCEKSIARLILGQTLTSGADGKSSTNALGNVHNEVRR
                     DLLVSDVKQLGQTFTQQIILPFLQINFGNIDPTRIPSFEFDTKEPADLALFAESLPKL
                     VDIGLPIPTQWAMDKLGIPEAQEGEAVLGRVAPLPTSGQAVGLSAHFVKSPPNPLFVK
                     EGEQITGCSCGCGGKVQALSAQPQTGIKEQDALDSLANEAFAVPDFNKQFDPITKKAV
                     AVVMACSSYDEAAEKLAAAYPDLVSEEHERYLANALFLADLLGAANAES"
     gene            23296..24585
                     /locus_tag="PWY43_000337"
     CDS             23296..24585
                     /locus_tag="PWY43_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL2135252.1"
                     /translation="MQKVNFAIGLEPKQAIEFLRQKKLLANKVFKQELFDSALGRATT
                     ISKLTSLEMTRDIYESMEKARREGKSFNEWKKTLTAEFERKGWVYGHDKAISRGIDGK
                     LLADPKTGEHFGTPRRLNTIYRTNMQQAYSAARYQRYMDNVDNRPYWQYSAVGDQRTR
                     PAHQALNGKIYRYDDPFWATFYPPNGFNCRCTVIALGERDLKRKGIDEVGSSEPFLVK
                     AKRPKDKLGNQEETIGFKLPDGTVRLADKGFDYNVGRLTYKPNLDLYPEKLAHQFAKA
                     EMNGGEFKQAFKKLEDAVNQAKGSHKKLSADEMVKVRNALNQNFKFAAGRLSEETQKQ
                     IDSKVGTVWLSDDTLIKQFNSREGQNFDVSDYANLPDIFNNPLKVELDERDGWRFYAE
                     LNGKRYFAVIKVLAQYNEIFVQSFRLVSDKQWAKVFN"
     gene            24733..25149
                     /locus_tag="PWY43_000338"
     CDS             24733..25149
                     /locus_tag="PWY43_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL2135253.1"
                     /translation="MIEIQINGIDEIIATLNRIATKTKDKTDLMRNIAGTMESAVLTN
                     FDEGGRPKWLGIKHRQGTPLVDTENLMGSITADYNNDSAIVGTNEPYAAIHQFGGMAG
                     RGRKVKIEARPFLQLTSQDRQDILDDVQAYWQKLLD"
     gene            complement(25146..25364)
                     /locus_tag="PWY43_000339"
     CDS             complement(25146..25364)
                     /locus_tag="PWY43_000339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135254.1"
                     /translation="MRKKSNGEQLPQVANFQKKVTAYIKQEMQPILKRFKSVLKNFKK
                     VGMINRTIKNKNATESDLGAICGDYLIA"
     gene            25408..26475
                     /locus_tag="PWY43_000340"
     CDS             25408..26475
                     /locus_tag="PWY43_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HDL2135255.1"
                     /translation="MFKQKFKLNPIALSFKLNQEANGRIQLCPFGWFEAQDGRGGRWY
                     VGDENGYALADDINNTAIDLMIDYEHQTLYIAENGKGNPAAGWITKAEYISGEGLFAD
                     VKWTPQATQEIKDGVYRYLSPLFLTDGSGMVIKVLNAALTNRPALHNLQEVVAMSSRF
                     APFLTPKEENPKMKELLIKLFALSANATDEQITEKLTALEKAKGDSPVALSDVYAELA
                     KEKGNVVALSAKVNNPDPAKFVALSDLQAVQTELNGLKQQINDKERDALIQTALSDGR
                     LLPAQKEWAENLGKQNLVALSDFLKTVTPNPALAGTQSGGEDPNKGAEKVALSAAEIA
                     TAKTLGLTPDEFAQKYKEEAK"
     gene            26475..27392
                     /locus_tag="PWY43_000341"
     CDS             26475..27392
                     /locus_tag="PWY43_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HDL2135256.1"
                     /translation="MFKKSELLKALDTAFKKEFGAGLALIKPQWEMVAMKISSSTKTN
                     TYGWLGQFPKMREWVGKRQIKKMQAEAMSIDNKTFESTVSVARTDIEDDQVGLFTPIV
                     QQAGQSAAELPDDLVFGLLAKGKETLCYDGQNFFDTDHPVYENVDGTGTSKTQSNITT
                     GAASGKPAFYILDDTNAIKPLIWQERTKPEIETKFDPSKSDTVFMEDEYLWGVRARGN
                     AGFGFWQLIHRVENSELNAETVMAVIAKMRMLKGDGGKLLNIRPSTILVPPTLEYAAR
                     QLVEADIINGTSNPLKGVLKVAVSAQIAE"
     gene            27438..27755
                     /locus_tag="PWY43_000342"
     CDS             27438..27755
                     /locus_tag="PWY43_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135257.1"
                     /translation="MMSRKKKDDETLNPKENTPPEDKVEEQPKPPEEETKPDDGAVID
                     PIAFEIKLKSIHPQATYGRCGYRFNKETAVEIARADLTDEQIITLANDPYLEFVPVVE
                     GDA"
     gene            27755..28180
                     /locus_tag="PWY43_000343"
     CDS             27755..28180
                     /locus_tag="PWY43_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL2135258.1"
                     /translation="MYALVDDFVLRIGEQESIELTDREMVGVVDEATLEVALADSSSQ
                     IDGYLSGRYRLPLKTVPQNLKRICCDLARYHLTSKSLVTMTEEVENRYKLCLKELENI
                     SKGVVSLAVDETNEEESATGENTVQFFNGGNRIWGRDKR"
     gene            28177..28818
                     /locus_tag="PWY43_000344"
     CDS             28177..28818
                     /locus_tag="PWY43_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HDL2135259.1"
                     /translation="MISKIERALIERLTLGLGKMVYAVAGYAGEINDEKLDVLRLPAC
                     LVSYGGSAFEAKSMGVRGKRYQATDVFVVLVITRSMRNQTAGRLGGVTSREVGVNQLL
                     GAVKTLLINQTLDGLVHPIQPKRIRTIWNNAEVKKEKLSAYAIEFEISYNECNVLDDG
                     RFPEGVEPNEQVFKQYQGKLDEPQGDLSGFNNRIFDPNNNAETAFRTEFKDGD"
     gene            28819..28998
                     /locus_tag="PWY43_000345"
     CDS             28819..28998
                     /locus_tag="PWY43_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDL2135260.1"
                     /translation="MLKVKAAKGVRVPVENRPHHYIEQEAVEVENTLYYQRRIADGDL
                     IVVKETKSAKETGGK"
     gene            28998..30407
                     /locus_tag="PWY43_000346"
     CDS             28998..30407
                     /locus_tag="PWY43_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDL2135261.1"
                     /translation="MSKIEFEKIPNSIRKPSVLTEYNNKDAVTTLPTNEQEVLIVAPM
                     LNGEAAFTAPQKIFSDVEAENLFGKGSVAHLMVRQAIQNNPLIRLTVVGLKDHEAGIA
                     ATGQVSFTGTVTYAGVVRITIAGTAYEIAAAKGEEAQAIVARLVNVINAASYSPVVAS
                     VESETTLKLTSKAKGEIGNEITLATRNTATGLTLEARAFDNGQRNALIAPALASVAGT
                     HYNVIISPFSDDENALALRSHLESVSAPIEDKPAIGVMGWRGTYATGTTLTASLNSER
                     IIVGWYKGATESNAMIAAGLGAVIAGEEDPARPLNTLEVKGLTVVDDSQKPLFSEVNQ
                     ALYHGLSPIEVVASRVQITRAITTYTKNVTNTDDPSYLDLTTIRTLDYVRKAIQTRQR
                     LRFPRAKNSHRIVAKVRSEILDVLYQLESEEVIENVEAWKNRLLVVRNQDPTYLDLEI
                     PADVVNGLHVIRNKITLIL"
     gene            30418..30792
                     /locus_tag="PWY43_000347"
     CDS             30418..30792
                     /locus_tag="PWY43_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2135262.1"
                     /translation="MSIVYEGSCVLEVDGVEIEITKLDVKTVTGRKLVKTMNSSGRAR
                     GYSQGIAEYTLSVTAVEPQDGTVIDWDNIKNAKLTKYPLDNSEKRTSYLGCFTTDVSE
                     SYTVDGESVRDIQLNALRKVNE"
     gene            30792..31160
                     /locus_tag="PWY43_000348"
     CDS             30792..31160
                     /locus_tag="PWY43_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135263.1"
                     /translation="MKITLNFGIEYNGKIRRDCTVRLLTMGGQCTAQEMIADLGFEEN
                     SEALSKREQTLVDMAYLSQQVTFDGIYQGNVTAQFLFDNLAPEDYWQLIEATLTLRKK
                     RLENGVSQSESESPANPDLA"
     gene            31190..31378
                     /locus_tag="PWY43_000349"
     CDS             31190..31378
                     /locus_tag="PWY43_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135264.1"
                     /translation="MILLSKFAITAEQVWQMPLLEVFAWIESYLESEGVKTPHSPMGA
                     NSEVKRESYVFTRCGKIE"
     gene            31431..33710
                     /locus_tag="PWY43_000350"
     CDS             31431..33710
                     /locus_tag="PWY43_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2135265.1"
                     /translation="MAKNMKVQLELTAKDTASPVLNQAAKTTEKAFKNTERAAQQSGN
                     AQTQAAQKAANATESSNRKIEQSYRNARRVAADVSRARETLGVRAENAIQREIEQTRA
                     AYDRLKRSGVASQNELRRASEATKKRVQELNAELGKTSFGDKAGSFGRGLMGAGVGIA
                     AGAAMARQPARNQMEFDRQLAMVANTAFSDRDVKGRIDGKRELLDAVQKAVSVGGGSK
                     EEALSSLDALLASGAVSTETALNLLPTLQKGAVATGASANDMAQIAISSMQQFGIKEE
                     DIGRVLDMAVAAGQAGSFELANMASWLPQQMAAAKQAGLSGIEGFERLLIANQQARVT
                     AGTSNEAGNNLVNLLGKITAKETNERFKNIEYKDPKTGKTKAIDFAKAMEHYKGKGQD
                     SLQAFMSIMDDVVGSDKRYQELQAKLKTAKGEDQKRLFNELTNLVEGTAIGEIISDRQ
                     ALMALLGIKNNVQLGEKVQKDIQNSEGAVETSHAVIRDTNSHKVEALKNATEFAQMEN
                     FEKVNGVLGTLAEHLTSYANQYPNLTQFVSGATDAVKIFGAALAASSVLDLLTGKKGN
                     DGGMGDLFDKSSKATGSKGKVSKFLSVGGSFASVAGVATMLHGDQAPMSEERKAQLND
                     PNYQARQMYEYKKEILGSRFHSFFRSNETLEQYRKDVSAYEAKQKNGNTMGAVQGVLS
                     TIPSLQELRPKVDSVTQTLSTYQADFQAFGATLSAGIEAGLASQSHTIANQITVELDG
                     SVVAEKVAEYQFNFNKRMA"
     gene            33710..>34812
                     /locus_tag="PWY43_000351"
     CDS             33710..>34812
                     /locus_tag="PWY43_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HDL2135266.1"
                     /translation="MSGWTMPIQQASFKGVRFDVLAVDDSFERAVIEHAYPFVNGADL
                     EDMGLNTQTIRLQAVFFGSSYYTDYIRLLSVLQQKGADVLVHPIRGRLPNMLLVSANL
                     HTDAENINYVALDLTFKEATEMQPIFAIEDSLITKIDRLLLDLENLFDDGLRFWDSVA
                     VFDLKSRLLGLWGALFASFEAVRNLFDLDKKKYSIASSTSQRTYLKDGSGALHQLKEM
                     VDSGIQGRADLTTLSFKSQLLNITDAVDNIQAIVSKVARDEGFNRSKTVKLLGSDVVE
                     LGIMLDLIATTKLTKVLTELIEDREEELLIGDLELINQIVREHWLNIINKLREQQKHA
                     QKGIMSDKTKSIYETAEKLINRIRNVAHQFTVLV"
BASE COUNT        10439 a         6581 c         8416 g         9376 t
ORIGIN      
        1 aaacgtaccc ttaaccagcc ggcagggcat ttagaaaaag atgaaacctt gctggagggg
       61 gcaagccgtg agctgtttga ggaaaccggt attcgggcag aaatggcaaa tttggtgaaa
      121 atttatcaat ggcaagcccc tcgttcacaa accgatttct tgcgttttct gttttcggtg
      181 gaactggaag agtggctgga gccgaatccg caagatgcgg atattaccca agccctttgg
      241 cttactttag cagaatttga agactacatt cagcaacaag ggcaatgtgc cagaagcccg
      301 ttggtggttc aatcggtcaa agattattta agcggtgagc gttatccatt aagtttgttg
      361 aatgcgtttg aatgggcgag aaatggaaaa taaaacaaca acccaatttg atgtggtgat
      421 tgtgggcggg gcagtgaccg gctcggtgct ggcattggcg ttaagtagtt ttactcatca
      481 caaaatctcg attgcgatag tggaaaaatc gctgccggac tatgaacagc aaggcggttt
      541 tgatgctcgc agtattgcct tagccgaagg cagtttgcaa aaaatggggc aaatcagacc
      601 gcttgcaggc tcaagtttag gcgagatgat tagcgaaatc ggcacgccga ttcagcaaat
      661 tcaagtagcg gataaagggc attttggtaa aacgaccttg aaagcaagcg agcagaatgt
      721 ggctcaactg ggtatggtgg tggaacttgc aaaattcggc agaaaattga ccgcttgtct
      781 tgcacagcaa gctaatatta cgcttttttg cccgaatcaa gtggttggca ttgagcgttc
      841 ccaacaacat tgtggattga gcttgaaaaa tggtgagaaa ctaagctgtt ccttgctggt
      901 ggcggcagat ggtattcaat ctcagattgc aaaacagtgc ggtgtggaaa ccgtaaaagt
      961 aagggattat gcacaatcgg caattatcgc caatgtggaa ctgagtgaat ctcaccaaaa
     1021 tcaagccttt gaatatttta cgcctcaagg cccttttgct ttattgcctt tgagcggaaa
     1081 tatgatgtcg ttggtgtggt gtgtgaaaga gccgaacgag ttaatccaac tttcagacag
     1141 tgaattttta gcccgtttgc aacaacaatt tggttggaag ttagggcaat ttgttcgagt
     1201 gagcaagcgg tttgtttatc cgctcatttt gcaaaaagcc cagtcgcata ttcaccaccg
     1261 cttagcagtt gtcggcaatg ccgcacaaat gcttcacccg gttgctgggc aaggtttcaa
     1321 cttaggcttg cgtgatttga ctaccttagc ggaattagtg gctttggcgt ttaatcaagg
     1381 gaaagatata ggcgaatttt cgctcttatc aacttttgag aatgagagaa cgcaagatca
     1441 agccagaatg ataaatgcta caagcggtct gatttcgctt ttttgttgcg aaatgttacc
     1501 agttcaggtt gtgaggaatt tagggttatt aacggtttcc cattcaaaaa tagccagaga
     1561 ttttgttgct aatcaggcat taggttggta aaaaacacaa aagcagcaat tttctatttg
     1621 acactgtttt taaaaaggcg tattatccag cccgtttaaa aatctcacca aagccttgaa
     1681 tgcaaattca aggctttttc ctttttagtg aaagcctatg aattttgttt gccgtaaaat
     1741 cggcaaaaaa attttgccta aaattcgcaa tcgtttgcga aataggttta tagcgttgtt
     1801 ttcatctggg taactgcaat ggttacatcg caatacatca aagagagtaa aaaatgacca
     1861 cagaaaatat gagtacgcca aagcaggcgt ttatcgggct gcaaatgctg tttgttgcat
     1921 ttggggcgtt agtattagtt ccgctgatta ccggtttaga tcctaatgtt gcccttctta
     1981 ccgccggtgt cggtacgtta atcttccaaa tttgtactaa acgtcaagtt cctgttttcc
     2041 tcgcatcaag tttcgctttt atcgctccaa tccaattcgg tgttcaacaa tggggaattc
     2101 ccgtcacaat gggagcattg ctctgttccg gttttattta tatggcatta tctgctttag
     2161 tgaaagcgaa aggggtgaat attctgcata aatacttccc tccaatcgtg attggaccgg
     2221 taattatggt gattggttta ggtcttgctc ctgttgcggt ggatatggca acgggtaaat
     2281 cggcgaatat tgcctataac accgctatta ttgtttcaat gataacgctg gtaactacgc
     2341 tgattgtggc ggtgtttgcg aaagggctat taaagctcgt gccaatatta gcaggtttaa
     2401 cggtgggata tatcttgtct gtttttatgg gattggtgga ttttactaaa gtggttgaag
     2461 ctccttggtt tagtctaccg aaagtttccg caccggaatt caaattagat gcaatcttgt
     2521 atttattacc tatcgcactt gctccggcag tcgaacactt aggcgatgta atggcaatca
     2581 gccaagttgc cggtaaagat tttatgaaaa aaccggggtt acaccgcacg cttttaggtg
     2641 atggtttagc aacttcagca gctgcggcat taggtggccc accaaatacg acttactctg
     2701 aagtaaccgg tgcggtaatg ctaaccaaaa acttcaatcc gaaaattatg acttgggctg
     2761 cgtgctgggc tatcgctttc tcattctgcg gaaaaatcgg ggcattcctt gccactatcc
     2821 ctacttttgt gatgggcggt attatgatgt tgctgttcgg tgcagttgcg gttgtgggga
     2881 tcaatactct aattaaagct aaagttgatt taagcattcc acgtaatctt tgtattgctt
     2941 ctgtagtaat gacattcggt attggcggta tgttaattaa catcggcgaa ttctcattaa
     3001 aagggatcag cttgtgtgca ttagtcgcta ttgtacttaa cattgtatta ccaaaagaaa
     3061 aagtagaaga ttctgctgct cattaatcaa aggacgctgt ggcgtccttt ttgctaataa
     3121 ccctctattt gtaaataaaa aggaataaca ctatgcaaaa actcacattt tcaccaccac
     3181 ttttaaatac gccttgccct tggtgttcgg agttagagag cttgcgtgaa ctttatgcgt
     3241 gtgaatatac cggtgcggta acaacccgta cttcaatgcc tcaaccatat ccacacgatt
     3301 gggcgaagaa ccaatatgta ctctttgatt ccaacgcaca gaaaacagca agtgtgaata
     3361 ctcaagatgc aactagctta caaacagcaa gccttaacac catcggttta agcccaaata
     3421 atctggatac caccatcagc tttgtacgca ccatttcaaa tgagctgaca gcgccatcaa
     3481 gcaaagcatt tattatttcg gtgtttggtt cacccgaaga ggtgggggaa tgttatgaaa
     3541 aaattgttgc tttccaaact gaagtgaaaa tgcctttggc aatggagatt aatatctctt
     3601 gcccgaatat tccgggtgaa atttcgcctg cttattccgc agaagaatta tcacattact
     3661 tgcacgcact acaaacttcg ctaaaaaaac tggctcaagg tgataatgct ttaccaatcg
     3721 ggattaaaat tccacctttt acttaccaaa atcaatatga tgaattaatc aaaggcttat
     3781 tacaagcggt cgaaaatggc gaaaatttac caatttcctt tattacttca accaatacgc
     3841 ttggctcatc gcttgtctta aatgctggca aagcggtatt aaattcagaa gtgggaacgg
     3901 gcgtaggcgg aatggcagga acggcaatcc atgcactttc tttggggaat gtttacacga
     3961 ttcgccaaat gttagatcaa gagcctaagc tcaaacatct tcaaattatt ggtgtgggcg
     4021 gcgtgaatga taaagccggc tttgagcgaa tgaaatcggt tggggcagac tttgtggggt
     4081 tagctaccgc attaggggtg gaaggaatcg gcgtttttaa agagattctg tctgaataat
     4141 gaagaggagg gcgtgcattg cacgcccgaa taaatgaaat tagcctaacc atttctccac
     4201 taattcatta gtggtttctt tatgtaccca catactgcca agttccggca ttgggaattt
     4261 ggtgtggttg tagccgacaa attggcggaa atcaaacacg gcgtgcgggt agccgttgat
     4321 cagtaaaaag gcttgatagc cgtcttcact ccagcaaatt tctaaacggc gaggttcttt
     4381 taatgaattt tgctcaatat cgctgacccg aaacacaaat aaactatcca ctaccggctg
     4441 ttcggtttgg tgtaagtcca ttgcataaaa gtagccgctt tcttcatcaa tttcaaacat
     4501 cactactaaa tgttcgtgct gggttgaatg agcgccatta cggtaaggtt cgttcagtag
     4561 aatatgagca gttaaaacag agtagagcat taaattatcc ttataagtaa aatgccaccg
     4621 atttctcagt ggcatttaaa tatttaatca ggcaattacg cttgaccttt aatctcttta
     4681 cggccgttga acggagccgg tgtaccagca cgctctaacg cttcttcgat acggattaac
     4741 tggttgtatt tcgctacacg gtctgaacgg ctcattgaac cggttttgat ttgacctgct
     4801 gctgtaccta ctgctaaatc tgcaatggtt gcatcttcag tttcgcctga acggtgagag
     4861 ataactgcgg tgtaacctgc atctttcgcc attttgattg cagctaaagt ttcagttaaa
     4921 gaaccgattt ggttgaattt gattaagatt gagtttgcga tacctttctc aataccgttt
     4981 ttcaagattt tggtattggt tacgaataag tcatcaccaa ctaattgtac tttgtcgcct
     5041 aatactttag tttggtatgc gaaaccttcc cagtcagatt cgtcttgacc atcttcgata
     5101 gacacgattg ggtactcttt gcataagcct tctaagtagt gagtgaactc ttgtgaagta
     5161 aaggttttgc cttcgccttt catatcgtac acattgcgtt ctttgtcgta gaattcagat
     5221 gatgcacagt ccatcgctaa agttacgtct ttacctaaca catagcctgc tttttctacc
     5281 gcttctttaa tgcacgctaa tgcgtctgcg tttgaagcca agtttggtgc gaaaccacct
     5341 tcgtcaccta cagcagtatt taagcctttc gcttttaata cttttgcaag attgtggaat
     5401 acttcagcac cgatacgaag agcttctttt aatgtagaag cacctaccgg ttgaatcata
     5461 aattcttgaa tgtccacgtt gttgtcagcg tgttcaccac cattgatgat gttcatcatt
     5521 ggtaatggca ttgagtaaac acccggtgtg ccgtttaatt cagcaatgta tgcgtaaagc
     5581 ggtaagcctt ttgaagcggc tgctgctttt gctgttgcta aagaaaccgc taagatagcg
     5641 tttgcaccga attttgattt gttttctgtg ccatctaaat cgatcataat ttggtcgatt
     5701 tcagcttgtg cagaaccgtc tttgcctact aatgcgtttg caatttcgtt atttacggca
     5761 gaaaccgctt ttaatacacc tttacctaag aaacgacctt tgtcgccatc acgtaattct
     5821 aatgcttcac gtgaaccggt agaagcacct gatggagcgg ctgctaagcc tacaaaacca
     5881 ccttctaagt gaacttctgc ttctacagtt gggttaccac gagagtcgat gatttcacga
     5941 ccgattactt taacaatttt agccattgtt gatgtcctct aatatacgag ttaatagaaa
     6001 aaagaatagg tatatagtaa agcgattttc tgcttttgtc ttggaaaaac tgattgagtg
     6061 gcagaaaatt tgccactaat ttgatctaag actatattcg cctgagatta tagcaacctt
     6121 tgcaaaaaac ggcaaaaatt tgcccgcttg tatgcttatt ttttgattaa atgtggggta
     6181 ctgatctttt ttgcatagag agaaagaaaa aaatttgggc ggaaaaataa cggcgaggct
     6241 aggaaataca aggcttcgcc gtttttgttt ggttggggct atgcaaaaaa acaaaatgag
     6301 caggattaga aaaaaatgag cagtttttgc gatatttttt gcataggttt gatcggtttt
     6361 aaaaccattt taaagcagtt ttaaacgaga gaatgcccga tatgtaccac ttgtccgatg
     6421 atttggaggt tttgagcttc ttcggcggta atctgtattg ggcggtagga ctcgttatcg
     6481 ctaattaatt caatatagtc ccattctttt ctaatccgtt taacccatac gctttcccca
     6541 ttctgcacaa gataaatctt atcatcacca cgaggctctc gtttgctgaa atctaccagc
     6601 atttggtcac cgtctgcaat ggtagggtgc attgatgttc catttgccca aaacacgccg
     6661 cagttgtggg cttttacgcc gaggctttgc agcaggctgt cggcgtaagg ttcttgcccg
     6721 tctggcttcg ttacgccctc gttaaagctg ccaaagcctg ctgaaacgtt gattgtgctg
     6781 aatccgttaa tcattgaaac aatatcatca gccatttttt ctagtcgctc aaactctctt
     6841 gccacaatac tggtattctc tgattgtggt ttgctttcct tacctgttgc cagccattca
     6901 aggctcacat ttgacacctc tgctattttt acaagatttg aacgagatgg atctgcctct
     6961 ccttttaccc acctagatag tgatgcttgg gctaccccca ctgctcttgc aaactcactg
     7021 taattgtcct taaagctatt ttttgcaatc catttcattc tttctgagaa gctctcatcg
     7081 taaatgtttg gtttactcat cttttacgtt tccattaata aagttttact cgtaaaacat
     7141 actcataaac cattgatttt caagtgattt gtgaataaag caaaaatatt ttaaatattt
     7201 ttatgtttta cggttgattt atttaaagtt ttacggttaa atacacacat cagatagata
     7261 aaaagaggtg tatcaaatga gtgtattaag cgacgttaaa aaaaccgcac agagagactg
     7321 gcatcgagcc gatattctgg cggaacttcg taaaaaaggt tggtcacttc gcactttggc
     7381 agatgaaggt aatgttagct ataacaccct gaaatcagtc ttagacaagc cttatcccaa
     7441 aatggaacgt ctagtcgcaa atgctatagg cgttgctcct gaagtaattt gggcaggacg
     7501 aaccgcagaa cgtatagaac gtaatcggcg accaacctta acgaataagt tctaatcata
     7561 aatgatcttt aacgtaaaag atacaaaaag gagcatttat gagtgagata agtttaaaaa
     7621 cacactattc agtagctgaa ttattaagtt ttaagttatc tagtttgcca agtgctcata
     7681 aaaatgtgct tgataaagct ataagagaaa actggcaatc tcaaaaacgc aaagggcgtg
     7741 gcggtggtgt tgagtatgaa ttagcctgcc tccctcaaga aatccaaacc gaaatccgct
     7801 cccgctttat gtcggcggtg gtggaagcca agccgaaaaa gttaccggta gtgcgtgcgg
     7861 aggtggagtt aggcaacctt actaccaaac agcgagaaat tgctgatgca cgaatggcac
     7921 tggtggcgta tgtgttggag ttggaaggct caatgagccg aattaaagcg ataacctacc
     7981 tttgcaacct tgccaaacaa ggcgaaatgc caccgcactt ggcggagctg gttgccgtag
     8041 caaatgctaa aaagacggca aaacgcacac tctcggtgcg aaccttaaac ggctgggtgg
     8101 tggattactg caaggcggag aacgccgagc aacggttgaa actgcttgcc ccacaggtac
     8161 ggcaggagac caagccagaa gaaatttggt ggctgagtgc ctttttgggg acgtatcgtc
     8221 agaaaaacgg catttgcctc accgaagcct accgagaatt tgaggtggaa tgggcgtatc
     8281 agtatgcaga caacccgcta ctgttggcac aatgccccag cctaagccaa gtacagcgag
     8341 cgatgaataa gttgccgttg tatgtgaaag agtacggcag acgcaccggc tcgcattaca
     8401 agcagttgct tagctatgtg aagcgggatt ggtcggtact tcgagcaaat gatgtgtgga
     8461 ttggtgacgg tcactcgctc aaactcaaag tggcccaccc gatacacggc aagccgttta
     8521 cccctgagct gacaatgatt gttgatggtg caggcagaaa ggtggtcggt tggtcgctgg
     8581 cactcagcga gaatgctttt gcagttgctg atgccctaag gcacgccatt agcctacacg
     8641 gtgtgccggc aatctactac tcggataacg gtggcggtga gaaaaacaaa tttttggatg
     8701 cagaggtgac ggggatgttg ccccgcctcg gtatccgaca cgaaacaggg attgcaggca
     8761 atccgcaggg gcgaggcatt atcgaacggt taaataaaac catcggtatg acgattgccc
     8821 gacagtttga aaccggttac gcaagcggtg cagatccgga aacagtgcgt aaaaacctgt
     8881 atgcggtgaa ctcgctggca aatgccaaag gggaattaac cccactacaa cgtaaagcac
     8941 aaggcaaatt gccaacgtgg cagcagttga ttgatgtgat ccagtctgtg attgattggt
     9001 acaacaacga gcatattcac tcggaaatcc gtaccacgcc ggcacgcaaa tatcagcaga
     9061 tgttgcacgc agaagatgtg gtgatgttaa gccctgttga gttgcgggat atgagccgac
     9121 cggaatttat ccgtaagccg gagcgaggtt gggtaagttg gaataacaac cactatttta
     9181 acctgaaatt actggatttt gaccgtgaag aagtggtgat cggggttgat attcacaatg
     9241 ccgagagcgt gcaggtgcgt accaaagacg gcagatttat ttgcgaggca atttggaacg
     9301 gcaacacccg agaggcattc ccggtggcga tggtggaaca gcagcgtaaa gaacgccata
     9361 aacgccgatc caaccttaaa cagcaacagc ttgatgagat taatgcggaa ttaaacccgg
     9421 ttttaaccat tgagcagaaa aagaggatta cgaaaaaaag ttagcagtgg gaggctaaac
     9481 gatgaaaaac caagaactta gagccttaat ggatagcaaa ggctatcaac aaaaacaagt
     9541 ggcacagctt ttaggtgtat ccgttgccac agtcagtctg tatctcaaag gcgattataa
     9601 cggcaatgtg gctgaaatgg atcgcaaagt agacgagttg attgagcggg ataaggcgaa
     9661 ggtggtggaa gcgaaatata acgctgcatt tgtgccaact ttagcggcac gcagagggat
     9721 ggaggtaatg cagtttgccc atattgaagg cgagattaat gtgatttttg gggcagccgg
     9781 tttaggtaaa acccaaatgc tcaaagaata cgaacgccga aacagctctg ccacattaat
     9841 tgaggttgat ccgagttgca cacctaaagt cttgctccgc aagattgccg aggcggtggg
     9901 ggcaaatgcc agaggggtaa ataacgaact attagaaagc attgtgaaca agttaaaagg
     9961 ctctgaaagg ttgctaatga ttgatgaagc tgaattactt tcgacccgct cgctagagtt
    10021 tatccgccga atccacgatt taaccggcgt gggcgtagtg ttagccggta tgccgagatt
    10081 gttggttaat cttaaaggta aaaataatga gctggcacag ctttatagcc gagtaggttt
    10141 tgcttgtgac ttaggcaatg ccttgagcga ggaagattta gggctgttag cagaaagtgc
    10201 gttaggtaca aatgaattta atgccccgct gattaaagcc tgcaaaggta acgcacgccg
    10261 attaagtaag ttaatgcgtg gtgtcgtacg gtctagcgag attaaccaaa cgccaattag
    10321 taaagatttg gttgagcaat atagcaagat gttgattagt tagggagcga attatgactt
    10381 gttttagaca ctggacacaa agagtaacag atggaacaga agatttgttt tggttggatg
    10441 agttgctagt tggtaaagat gagagagatg gtagttttac actttctatc tttgatcctg
    10501 atatagatga ctttttgtta tatgagaaag taaaaacaag aaaagattgg gaaatagctt
    10561 atcgaaaagc cagagaaatt attaatgaac aggacataga ttgtagaagc tttagagttt
    10621 aaggggaaag tatgcaaaag gtatttagcg aaaaagcagg cgaaatgatg aacccattta
    10681 acgcttatat ccacgacagc cttgtgaagt tagaaattgc cacgcttgag tgcgaggcgt
    10741 tggatttgga aattgagcgg gtggaatttg aggcggcaag cccacctcgc cttgtgttgc
    10801 gagataaccg caaaacgcag gattttgtga aaaacggctt agcaagccta tttggaatga
    10861 aaacaaaagg cggcagacgg tatgacagct accaaatgca ggtgcggggt gtgaaatgcg
    10921 tgtgggagtc tgaacgtgtt acgaaggtgt agaaatgaaa aaacggcgaa tgatttatgc
    10981 cctgtatgtc ggcgaagaga atgtggctga cggcacggcg gaagaattag caaaacggtt
    11041 gggagtggaa gcaaagacga tccgatttat gtcgtcccct gctcataaca aacgagtgaa
    11101 gtataacggt aaagcagtcc gcaccgtgaa agtcgggttg gaatagagag gaagtattat
    11161 gagtaaagta acaatcggta atgaaattta ctggaaagat gctgatggca atttaaagcc
    11221 ggaggcatta gtaaaagaaa tcgacaaaga acgtgatgaa ttagttcgcc aatttgtaag
    11281 taaagcacaa gcgttaagca aagcgttagg cgattttaaa caatccgtat ttgatgacgt
    11341 aggggctttt gtcagcctat cggctgaaaa atatggagta aaaattggcg gtgcaaaagg
    11401 caatatcaca ctatttactt atgacggtga atataagttg cagttagcag tgcaagagaa
    11461 tattcgcttt gatgagcgta ttcacgcagc aaaagccttg attgatgaat gcttgcacga
    11521 ttggtcggaa ggggcaaaac cggagcttaa agccttaatt gataatgcgt ttgaggtgga
    11581 taaggaaggc aatttatcta cggcgaaaat cctatcccta cgccgtgttg agattgatga
    11641 tgcacgctgg aatcaagcaa tgacggcaat ttcagacagc gtgcaggtaa ttggtagtaa
    11701 aggttatatc cgtttttata aacgtgatga aaatggcaaa tatcagccga ttagtttaga
    11761 tatggcgggg gtttagaatg tttattactt ttaatgttaa ttatacggat aaacctgttg
    11821 tggttaatac agacaaggtt tgttcaatag aaaatattaa cgggaatgta acagtacact
    11881 tttgtgataa cacaaaatta ataatgatgg actttgatga taaagaatat gtatcgttat
    11941 taaatcattt gcatatttta aatcagttaa aaagaaagtc ttagttaaaa aaccttttca
    12001 atgcccttta aaaatctccc ctagcccctc tttttcaaag agggggacaa gatagaaggg
    12061 catttataaa gtgttttaac cacaggagga aaaaatgaaa acagaacaaa aggcgactaa
    12121 gtttgaccgc tttcggtact atgccgagaa agccgcagaa gcagaacgca aaggcaatta
    12181 tatagaagcc caagaccact gggaagttgc aaaattatcg gcaaaatcga ccgctaacct
    12241 tggctgggcg gaacaacggg cggaattttg taaacgtatg cacaacaagc catttgaagg
    12301 ggaataaata tgatgaccga acaagataaa aacgaactta acagccaatt aaacgaggcg
    12361 ttaatgcaga ttattcaagc ccaaaagtat ttaaaacaaa gtgattttat tcgcagcggc
    12421 gtgtatctcg gcacggtgca agatttgttg ccaaaggtgc atttgaagtt attaacggca
    12481 aatcgtaaac attaactaaa ctagcaagga gttaaaaatg aaatttagat tttttaaagg
    12541 ggatataacc caagagccac taaaaagcat ttatgcagat tatcaagtat ctaaagaaga
    12601 acgcaaggat aagctcaatg ctattttaga aaaatatcct ttcaatgatg gattaattag
    12661 taggtcaggc tggtttcata gaatagttat agggattgct tgtaaagcaa ccaatattag
    12721 ccaagtttta ggtgttaaag ggttcaaggt cagtaaattt gatgatgagt tttacctcgt
    12781 caaacctgat aaacgttaca aaaaagggaa agaacttgca gaagatttta aagcgattaa
    12841 tgaaatttat gaacaacatc gtgatttctc accctacatc ttaaagcgtt tgaatatgtt
    12901 ttattttgca agtgatttta gttattcccc aacatcaggt cgaagttatt tagccgtagc
    12961 agacattttt gaaaacacct tactggttaa agtgccaatg cctattgata aaaatgatca
    13021 accatttcct gatattgcag agggattggt tgagattaaa gaaagtgagt ttttagcaat
    13081 acaggggaaa taaaaaatga cagaaatgca acaacaaatc attaaatggc tagcaaatgg
    13141 tgaaacaggg acaagtagca aaacaatggc atttgtaatt ggttttgatg ttatcccaag
    13201 tcgtagcggt taccctcacg acgtgtctga tttccgcctt tgttttcagt tggtagaagc
    13261 cgctccagaa atgcgtaacc atatttacaa gatgaaggca atcagcccaa tttggtcgga
    13321 atttgccgaa aattgggaag agttagaaag aagatataga gcggagcaag gtcaagggag
    13381 gttaccgcac acctatgcct taattcaaca gattactgat aaagatcaga atgtcataag
    13441 aatggggaat gtgagttttt catttggtca ataagcaaaa cccatttaca gcccatttaa
    13501 cccctattta agtgggctga ataatgtgtt ttataggagc ataaaatgga atatcaaaaa
    13561 atgcctgaat atcggttatg taaaaagaaa tcgggagata ttgtgttaca acaattagtt
    13621 atttgcgaga taactccagc aacttggcat aacccatacc ctgatagtcg aacttattgg
    13681 gttgatctcg aaacaataga agaaggagca gaatgatgaa actatgcaaa tgcccgattt
    13741 gccattcgga aatctcgctg gaggcgttga ttgaagatga tgcggggcgt gagttgttaa
    13801 atacgatagt caatatcggt gggaattgtg ggcgtgcagt ggtggcttat ttagggttat
    13861 ttaagccaca gaaaaacagc cttgccaata gccgagcctt gaaaattgtg cagagtttgt
    13921 tggagcttta tacaccctct aatgtgttag aaaaagcctt aatggatacg gtggagcaag
    13981 tccgccgaaa tcgtagagaa accggtagat gtgagccgct ggcaaatcat aattacttga
    14041 agaaagtata tgaaagcgtg aagccacaat ttgcagtagt aagaagtgaa gcgaaagcag
    14101 tgagtagcct tgagcaacag caacgccaag cagaccaaaa gaaacacgat gcggtgatgt
    14161 atgtggaacg aatgaagcaa atggggcaac tggaagccgt taagaacacc gaaagttatc
    14221 aaatttggct gaaatggcag gaggaaaagc aaaatgcaac cgcaaactag aaagcagatg
    14281 atacagaaaa tccatatcgg taaaaatgag ctaaaactgg ataacaacgc ctacaagatg
    14341 ctattgctgg aagcggtgga taagccgagc tgctcaatga tgaccgacag cgaattaatg
    14401 accgtattgc aaacaatgaa agccaaaggg tttgtggtga agtcgaaaaa atacggcaaa
    14461 cgcccaacag caagtaaaac cgcccctcac cggcaagggt tgataaataa aatcggggcg
    14521 ttgctttatc aaagccaaaa gccgtgggat tacgcccacg ccattgccaa acgctcgttt
    14581 ggtattgagc gggtgcaatg gctgaccgat gagcagttgc ataaattagt gcagatgttg
    14641 gcgagttata actacaggca cggaatgagt gtatagaatt tatttacagc cttctgcgga
    14701 gggctgaata ataaatttta cgaaatattt tgcatagtta tgatttttgg gcttgactgt
    14761 caagcggaaa aagtttattt ttcgacaacg atctcaaaaa tcgacaatac agcttgcacg
    14821 gctgaaagta tgtgctattt ttttgcctga atatcagaaa gcctttgagc ttcggaatat
    14881 ccgtgtagaa aaataaatat aatttaaatc aatgaccgac agtgcgagga atacaagacc
    14941 cgaaagggga ataactccgc tgtttattgt gcagttttga gctgtcggtc gccctactca
    15001 aaatagggtt tccaattcaa ggaaaaacac aatgaataac ttccaaattg caacctttac
    15061 gggttctctt caaaatcaat ctaccttact aattaatgca cgtgatctac atcagatgtt
    15121 acagattggg cgtgattttt ctaactggat taaatcaaga attgaaagct acggctttgt
    15181 tgaaaatgaa gattttactt gctcgccaaa tttggcgagc gagaaaagca aaggtttagc
    15241 cagcttttgg ggcagtcaaa ataaaattga ctatcacatc accctcgata tggcaaaaga
    15301 actctgtatg cttgaacgct ccgaattggg gcaacaggca cgccgttact ttatccaaaa
    15361 ggagaaagaa gccaacgccc tgaaacaagc ccaacccaaa gccacaatcg aaatcagcac
    15421 cgataaatac attgagctac tcgaaaccca aaaccaactg cttaaacaag cgatgaaacc
    15481 gcaaaagtac aagctacgcc taagtgccga agaaaaaagc catattttcc gcctgcacca
    15541 gcagggatta agcacagggc aaattgtgca acaatcgggg cgttcagaaa gtgccgtgcg
    15601 tgcggttatt cgtgaaaatt taggcaaata aggagtgggt tatgagaaca gaatttgaag
    15661 atattgtgca aaataccgaa aatttggcaa gtggcttgta tagtttaagc catttactgg
    15721 aaattttgga taataccgaa gaccaaattt caatgagtgg cttagcccat cttgtcgcaa
    15781 tcattgccag tgcgactaaa aaacaagcaa atgatgcagc aacagcctta aattgtgtcg
    15841 ataattaaaa atttacacta ttttttgcat agcccacttt ttaaagtggg cttttttgtg
    15901 gtagatttta aacaattcag gcagagaatt gagggttaag atgttctcat ttattaaaaa
    15961 attatttgat aataaaccat catctataca aaatattgaa gagccaccta aggaaataga
    16021 atcagaattg acatcatacg atgagaccat aaaaactctt tttgagaaat ttttagtcaa
    16081 ttattctatt cctgaaagac ataattacaa tgtcagaaag cgagtaagta aagaaattat
    16141 cttctctcaa ttattagaat ctgatagttt tagtatgcca gaagataaaa gagataggct
    16201 atataatgcc ctagttattt cagagagcgt agattatcaa gaattacatg ctattttttt
    16261 gctagatgat ttcaaatgga ggctatttga taattgcaga gagttttgtg aagagaataa
    16321 aatttatcca tatgcatatc aatttttagc aaataaacca gctatccctg ataatttaga
    16381 aagtgcgtta ctttttgtaa aagtttcaca acttagagag ttgttaaaag ataaattatc
    16441 ccaaaaaatc cctacaagaa aagatgaagt ttatcctcta tttgctcaac actttactct
    16501 cgaagagatg aaagaaatcc ttgaagaaag attcattgag gtaaaaaatc aatttgagaa
    16561 tacggttaaa aatcataaaa ttaatatatt gggacaatgg tttacatatt attcgaataa
    16621 cttgtcggat tattacctct acagaatttt ggatagaaca ctaacacatc ctaaactaga
    16681 tgtcccagaa tttataaggc atagctacaa agatctagaa tttataaaaa aggctggttt
    16741 aagttgcttt gatgaaaaag gaaatttgac aagtgtccct cctttattcc ctggtgatta
    16801 ttccggaata aactatggga attattatta aagtataaga ccgcttcaag cggtcttttt
    16861 ttatgcaaaa tttgcaaaaa aatttttaag taagtattac agatttgaat aacccccttg
    16921 gataatggct tttgtggaaa ttattcaagg ggatttttat gtctgaattt gaaagtgttg
    16981 agcattattt gcctgaatct gtaaaggaaa ttgttgattt gattggcttg tctgcaaccg
    17041 agaaactgat taaagccttt ggaggctttt cgttccagtt ttccaagggg aaggtttatt
    17101 ttgaaaaact taaagatgtg ttaggagaag aagatgcaga aaaattatac gattacttaa
    17161 gatctaatat agcttatttg cctcgttgcc aaacagcctt gcgattattg cgtaatgagc
    17221 gaatttatac cgattattgc cgaatgacag aacaagaggg aataagcggt cgtttggcaa
    17281 ttatgcagat ttgctcaaaa tacggtgtgt ccgaccgtat tgcgtgggat gccgtaaggt
    17341 atttaaaatc taaacctaag caacctatta cgcaaacatc acttttttaa tttgtggaac
    17401 tctttcatct cccctatctt acattaaatc attaaagtct ctttaaatca tcattgaatt
    17461 taaagaggct tttttatgac tgaacctatc aaaaacaagg caactattgc tgactttaac
    17521 cttgcatttg accgtcttat cggacacgag ggcggttatg tgaatgaccc gaaagacccc
    17581 ggtggcgaaa cgaattgggg tatcactatc catactgccc gtgcgaatgg ctataccggc
    17641 tctatgcgtg caatgagccg tgcggatgcg aaaactattt acctcaaagc cttttggcaa
    17701 cgttataact gtgagcagtt cccgcctgag ttggcttttc agtttttaga tgcgtgtgta
    17761 aaccacggtt cgggcaatgc tagccgaatg ttacagcgag cagtaggtgt ggctgatgac
    17821 ggcattatcg gcaaagtgac tttggctgcg attgagcgtt attcagtggc tgatgtgggc
    17881 ttgcttttcc aagcggaacg ccttgagttt tacaccaagc tcaaacactt tgaccgtttt
    17941 ggtagaggtt ggattaaccg aatggcgggc aatttgcgtt attttgccaa agacacggtg
    18001 gaggaataat cgtgaaacaa tttttacttg atttaattac caataaagac ggcacagcca
    18061 gcacgactgg ttttattcag tttatcagtt ggctggtgct gtcgggcatt ttaattcacg
    18121 cctaccttta cgataagccg tttatttccg actggtggtt tgcttatgcc ggtatttgtg
    18181 tgctgggcag ccctgccaca aaaggcgtgg taagtgcgtt aaaaaaagac cgaacaaagg
    18241 gagatgaaga atgattactc aacaaattgt cggttttatc ggtgtgattg gcacagtaat
    18301 tgcctatgcg ttttttaaag gctggcaatt aaagcaggag cggaaactta ccgctacatt
    18361 aaaggcggaa aagcagcaac aagcggtcga aattcaacaa aaaaatgcag aggttaaaaa
    18421 tgctcaaatt caacaaaaac acattaacga aactcgccgt attcagccta gtgccgttga
    18481 tgagcggttg caccaacacg gttacttccg tgacgactac gggttgcacg gcgttcggtc
    18541 tgatttatcc gagccgtgcg gacacaatgg aaacgaaacg ccaagtgctt gcacacaatc
    18601 tgacctatga ggaagtttgc cagaatgaag ttaattaaaa cactcagaca gcgttggcaa
    18661 caatggcgtt tttaccgccg ccgacctgac cttgcagaat tgcataacgt ggtgcgtgcg
    18721 gcggcagcac aaggtaatca acacccggtc gggcgagcca aaagggggcg ttatgattga
    18781 tgaaaaactg tttcagatgc tgatctcttt tgtagttgct ccgttagtgg cgtttgtagg
    18841 caaggtcata tttgatcgta tcaaccgcaa tgagcaaacg gtaaaagagc taaaagcaga
    18901 aatggagagc aaatatcaat ccaaagagct tgcccacgaa gtgaatagag ggattaagga
    18961 aaagctagac gatattttag actcgctcaa agaagtaaac caaaaacttg ataaaaaggc
    19021 ggataagtaa tgagccgtaa aaaacaaaag caaaccgatt cggaaaaatt agacctgatt
    19081 ttaggtggat taaatgagct taacgataag gtggataaac aaaacgaaga aatcgaacgc
    19141 ttacaccgtg aaatccaagc gactaatcgt ttagtgaatg aaatggcacg caaaaaccgc
    19201 cgtgatgccg tggttgccgg tggcttgggt ggcggcattg ttgctgtggg ttttgagttg
    19261 ttacgcctga aatttggaat gtagagggcg tatggcacac gatgttgaag tacaaaaagc
    19321 ggttaggcaa gcctatgtgt ttgaccgttt aagcctcgaa atggcagccg agcgtgccgg
    19381 tgtgtcgttt ggcacagctc gccgttggaa agccaacgct gaaaagaacg gcgataactg
    19441 ggaaaaagcc cgtgatgtgc aagcaatggc aagtggtggc attgagaata tcgctcaagg
    19501 cttgttagcc ggttttttaa ttaagtaccg cacgttgatg acggagcttg aggaaaacac
    19561 gaaaatgacc actgaggcga aagtggaagc attagctgct ctggctgatt cgtttgcgaa
    19621 gatgacggca tcgagcaaga aactattgcc tgaaacaagt gcaatggcaa ccgctatgcg
    19681 tgcgattgag atgatggcga atatcgtcaa aaccaagaaa ccgcatttat tacctgactt
    19741 tttagaaatg ctggacgatt tagaagtgca gtttaaaaag gagtttaagt aatggctaag
    19801 aaaaaacaaa ctgtggtgca taagcattac tctttccgtt ggattgtcca acttgcttgt
    19861 attctcggag caataaaact aatgctcaac ggtacagacg gttggggctg gttgttgctt
    19921 atcgcagttt gtttgtaatt tatgaaaatc aaagaatttg aaaaagaact ggaagaatta
    19981 cggcgacagt tacaacaaaa catcgaagcg agttttgagg gttgggacga taaaccacaa
    20041 gccattagcg aacgccgcca aaaggtttta gaccctgttc acggctttga gtattttgtg
    20101 caggcttatt ttccacacta tgtgcggtct gaacataaat cacagttgca tcattatctc
    20161 tttgagaatt taccgctttc ggtaagtgat ctttctaagt cagtccgcca agcgattgca
    20221 gcccctcgtg gcgaggcaaa atcaacgatt tgtacgcaat tattcccgct gtggtgttta
    20281 gtccgtgatt taaaaagata catcattatt gcgatggaca ccaaagaaca ggcatacagt
    20341 atgcttgaag ctattaaagt ggaaattgaa tctaaccctc gtttaagaat tgatttcccg
    20401 gaggttgcac aaggcaaggt ttggcgtgca ggtgtcatta tgacggctaa aaaacaaaaa
    20461 gttgaagctg tgggggctgg gcaaaaattg cgtggtcgtc gccacggagc ttatcgtccg
    20521 gatttagtag tgcttgatga tattgagaat gatgaaagcg tagaaaaacc tgcacagcgt
    20581 aataaattgc ataactgggt tttaaaagca gttttaaaac ttggtgctgc aggtgaaaag
    20641 tttgatgtaa tttatgtcgg cacaattcta cattatgaca gcgtacttaa ccgagtttta
    20701 agcaccaaag gctggaaaaa agcccatttt aaagcgattt tacgcttccc cgacaatatg
    20761 gcgttatggg acgaatggga gaatatctat ctttctgaag agggcgatga tgacacgctt
    20821 tcagacctat tctatcagca acataaagct gagatggata aaggggctgt cgtttcgtgg
    20881 ttggctcgcc ctattctcta tttaatgaaa attcgtgcta gtgatgggca tagttctttt
    20941 gactccgagt atcaaaacga ccctgtttcg ggtgatgatg cgatttttgc caatagtatt
    21001 caatactgga cggagttgcc cgaaaactta atctattttg gggcattaga cccttcaatg
    21061 ggtaaggctg gagcaagccg agacccatct gcaattttgg tgggcggtta tcaccgtgag
    21121 agcggtaagc tctatgtggt tgaggctcaa attaaaaaac gattgccgga tttaattatt
    21181 gaggacgtga ttagactgca ttcgcaatat aactgtcacc gctggtttgt ggagacggtg
    21241 caatttcagg agtttttgca gacggaactg gtcaagcgtt ctgcccagcg tggtaagccg
    21301 gtgccggcaa cggcaaccaa gccgaacagt gacaagatgt tacggattga gagcttgcaa
    21361 ccgcatattg ccaacggttt gattttgttg caccgctcgc aatctacgct tgaaagccag
    21421 cttcgccatt ttccgaaagc cgatcacgat gatggtccgg atgcgttgga aatgttatgg
    21481 cgaaatgcac tcagcagctc tgccccgatt gagtgggaga gtgtagatga ttttgacggt
    21541 gagtatcgca gtaagtggcg gcattaatgt aactcccctc tttgctaaag agggggacta
    21601 tattgagcgt aactaagctc tggaaaggaa aatttatgag caaaaagaag cataacaagc
    21661 agattaaacc gaatttaaac ccgattaaaa atcaagaatt gcagacggat ttagccgaaa
    21721 ttaccgctac aggtcgggta ctctccgacc atccgagcaa ttttattacc cctgctaaaa
    21781 tgaaagcgat ttttgaagat gcggagaacg gcgatattac cgcccagcac gagctgttta
    21841 tggatattga agaacgggac agtacgattt ttgccaatat ccaaacccgt aagcgtgccg
    21901 cattaggcgt agattgggcg attgttgccc ctcgcaatgc cacaccgcag gaagaaaaac
    21961 tgcgtgatga ggttgatgag ttattttatc agctcggcaa ccttgaagat tttgtgatgg
    22021 actgtatgga cgctgtggga cacggttttg ctgccctcga aattgaatgg gcgtttaacg
    22081 gtaaattgtg gtatccgaac gcctttatcc accgtcctca atcgtggttt aagtgggata
    22141 aggtagataa tctgttgctg aaaacccgac aaaataccga aggtgagcca ttacgagaat
    22201 ttggctgggt ggtgcatacg cataaatccc gctccacaca ggcggcacgc aataatttat
    22261 tccgctcgct ggcttggctg tatatgttta aacactactc tatccacgac tttgccgagt
    22321 ttttggagct gtatggtatg cctatccgca ttggtaaata cggtgcaggg gcaacaccaa
    22381 acgaaaaaca aacgcttaaa cgtgcattag cggaaatcgg acacaacgct gccggaatta
    22441 tgccggagtc gatgagtatt gagttgcata acgccgctaa tgcaggcggt gcagccggca
    22501 ataatccgtt tttgcaaatg gtggattggt gtgagaagtc tatcgcccgc ttgattttgg
    22561 ggcaaacatt aaccagcggt gcagacggca aaagctctac caatgcactc ggcaatgtgc
    22621 ataacgaagt gcggcgtgat ttgttggtgt cggacgtgaa acagctgggg cagacgttta
    22681 cccaacaaat tattctgccg tttctgcaaa ttaacttcgg caatatcgac ccgacccgca
    22741 ttccgagttt tgagtttgat accaaagagc ctgccgactt ggcgttattt gccgagagtt
    22801 tgccaaaatt ggtggatatt ggcttaccga ttccgaccca atgggcgatg gataaattag
    22861 gtattccgga ggctcaagag ggcgaagcgg tgctggggcg tgtcgctcct ctgccaacat
    22921 cggggcaagc ggtgggatta tcggcacatt ttgttaaatc tccccctaac cccctctttg
    22981 tcaaagaggg ggaacagatt actggctgtt cttgtggttg tgggggtaag gttcaggcct
    23041 tgtcggctca accccaaacc ggtattaaag agcaagatgc gttagatagc ttagccaatg
    23101 aagcctttgc cgtgccggat tttaacaagc agttcgaccc catcacgaaa aaggcggtgg
    23161 cggtggtaat ggcgtgtagc tcttatgatg aggcagcaga gaaactagca gcagcctacc
    23221 ccgacttagt gagtgaagaa cacgaacgct acttagccaa tgcgttattt ttagcggatt
    23281 tattaggggc tgcgaatgca gaaagttaat tttgccattg gactagagcc taagcaggcg
    23341 attgagtttt tacggcagaa gaagttgctt gcgaataaag tgtttaagca ggaattattt
    23401 gattctgcat tggggcgagc caccaccatt agcaagctca ctagccttga aatgacccgt
    23461 gatatttatg agtcgatgga aaaagcacgg cgtgagggga aatcctttaa tgaatggaag
    23521 aaaacgctta cggcagaatt tgagcggaaa ggctgggtgt atggacacga caaggcgatt
    23581 agtcgtggta ttgatggcaa attacttgcc gacccgaaaa ctggcgagca ttttggcacg
    23641 cctcgccgct tgaatacgat ttatcgtacc aatatgcagc aggcttattc tgcggctcgt
    23701 tatcagcggt atatggataa tgtggataat cgcccttatt ggcaatattc ggcggtaggc
    23761 gaccaacgca cccgccctgc tcatcaagcg ttaaacggca aaatttatcg ttatgacgat
    23821 ccgttttggg cgacctttta cccacctaac ggctttaatt gccgttgcac ggtgattgca
    23881 ctgggtgagc gtgatttaaa acgcaaaggg attgatgagg tcggtagtag tgagccgttc
    23941 ttagttaagg caaaacgccc gaaagataag ctcggcaacc aagaagaaac cattggtttt
    24001 aaactgcctg acggcacggt gcgattggct gataaagggt ttgattataa cgtggggcgt
    24061 ttaacctata agccgaattt ggatttatac cctgaaaagc tggctcacca atttgctaag
    24121 gcggaaatga atgggggtga gtttaagcag gcttttaaga agctagaaga tgcggtaaat
    24181 caagccaaag gcagccataa aaaactctcc gccgatgaga tggttaaggt gcgaaatgcc
    24241 ctcaatcaga actttaaatt tgcggcaggt cggttaagtg aggaaactca aaagcagatt
    24301 gatagcaaag tgggtactgt atggctttct gatgatacgc tgattaagca attcaatagc
    24361 cgtgaggggc aaaattttga tgtatcggat tatgcaaatt tacccgatat ttttaataac
    24421 ccattaaaag tagaattaga tgagcgtgat gggtggcgtt tttatgcgga attgaacggt
    24481 aagcgttatt ttgcagtgat aaaagtgttg gcccaatata atgagatatt tgtgcagtcg
    24541 tttagattag taagcgataa gcagtgggca aaggttttta attagccgct aggtagggct
    24601 cgaaccacct acacacagtc cagagtctgt tgcacctcgt cgcttgcgat cctcgagatt
    24661 atcatcgctt ttctagcggc tgtttaaaat atacccctgt ttattttaaa aatcaactca
    24721 gatggttaaa aaatgattga aatccaaatt aacggtatag atgagattat tgccactctc
    24781 aaccgtattg cgactaaaac caaagataaa actgatttaa tgcggaatat cgccggtacg
    24841 atggaatcgg cagtgctgac gaattttgat gagggcggtc gcccgaaatg gctgggtata
    24901 aaacatcggc aaggcacgcc gttagttgat acggaaaatt taatgggcag cattactgct
    24961 gattataata atgattcggc gattgttggc acaaatgagc cttatgctgc tattcatcaa
    25021 ttcggtggta tggctgggcg tgggcgtaaa gtaaaaattg aagcccgacc ttttttacaa
    25081 ttaacctcgc aagacaggca agatattttg gacgatgtgc aagcctactg gcaaaaactg
    25141 ctggattaag cgataagata atcgccacag atagccccta aatcgctctc tgtggcgttt
    25201 ttgtttttta tggtacgatt tatcattcca acttttttaa aattttttaa aacggattta
    25261 aagcgtttta aaatgggttg catttcttgc ttaatataag cggtcacttt tttctgaaaa
    25321 tttgcaactt gtggaagttg ctccccgtta ctcttttttc tcaatcttct attctttagc
    25381 ctcattaatt tgttttgagg tgtttttgtg tttaagcaga aattcaaact aaaccccatt
    25441 gcattgagtt ttaaactcaa ccaagaggca aatggacgta ttcaactttg cccttttggt
    25501 tggtttgagg ctcaagacgg gcgtggagga cgttggtatg taggtgacga gaatggctat
    25561 gcgttagccg atgatattaa caacactgcg attgatctga tgattgatta tgagcatcag
    25621 accctgtaca tcgccgagaa cggcaaaggc aaccctgctg cgggttggat taccaaggcg
    25681 gaatatatct cgggcgaagg cttgtttgcg gatgttaagt ggacaccaca agcgacccaa
    25741 gagattaaag acggcgttta tcgctatctc tcgccactgt ttttgacaga cggcagcgga
    25801 atggtaatta aggtacttaa tgccgcctta actaaccgcc ctgccttgca taatctgcaa
    25861 gaggtggttg cgatgtctag ccggtttgcc ccatttttaa ccccaaaaga ggaaaatcca
    25921 aaaatgaaag aactcttaat taaattattt gcattgtcgg caaatgcgac cgatgagcag
    25981 attaccgaaa aactgaccgc acttgagaaa gccaaaggcg acagcccggt ggcgttgagc
    26041 gatgtgtatg ccgagcttgc caaagagaaa ggcaatgttg tggcgttatc agccaaagtg
    26101 aataaccctg acccggcgaa gtttgtggcg ttaagcgact tgcaagcagt gcaaaccgag
    26161 ctgaacggct taaaacagca aatcaacgat aaagagcgtg atgcgttaat tcaaactgct
    26221 ctctctgatg gtcgtttatt accagctcaa aaagagtggg cggaaaactt aggtaagcag
    26281 aatttagtgg cgttatccga ctttttgaaa acggtaacac ctaaccctgc tttagcaggt
    26341 acgcagtcgg ggggtgaaga cccgaacaaa ggtgcggaga aagtggcgtt atcggctgcg
    26401 gaaattgcaa ccgctaagac gttgggctta accccagatg aatttgctca aaaatacaaa
    26461 gaggaagcga agtaatgttt aaaaaatcgg aattattaaa agccttagat acggcgttta
    26521 aaaaagaatt tggtgcaggt ttagctttaa ttaaaccgca atgggaaatg gtggcgatga
    26581 agattagttc ttcgaccaaa accaacactt acggctggtt gggtcaattc ccgaaaatgc
    26641 gtgagtgggt gggcaaacgc caaatcaaga aaatgcaagc ggaagcgatg tcgattgaca
    26701 ataaaacctt tgaatcgaca gtcagcgtgg ctcgtaccga tattgaagac gatcaggtcg
    26761 ggttattcac cccgattgtt caacaagccg gtcaaagtgc ggcggaattg ccggatgact
    26821 tggtatttgg cttgttggca aaaggtaaag aaacgctttg ctatgacggt cagaactttt
    26881 tcgacactga tcaccctgtt tatgaaaacg tggacggcac tggcacgagc aaaactcaaa
    26941 gcaatatcac caccggtgca gcaagtggca agcctgcgtt ttatatttta gacgatacca
    27001 atgcgattaa gccgttaatt tggcaggaac gcactaagcc tgaaattgaa accaagtttg
    27061 acccgtcaaa atccgatact gtctttatgg aagatgaata tctgtggggg gtgcgtgctc
    27121 gtggtaatgc cggctttggt ttctggcagt taatccaccg cgtagaaaat tcagagctga
    27181 atgcggaaac cgtaatggct gttatcgcta aaatgcgtat gctcaaaggc gatggtggca
    27241 agttgctgaa catccgacca agcacgattt tagtgccgcc aacattggaa tatgcagctc
    27301 gccagttagt tgaggcggat attattaacg gcacaagcaa cccattaaaa ggtgtgctga
    27361 aagtagctgt ttctgctcag attgcagaat agtaagttaa tcgggcgtag caatacgccc
    27421 cttttgagag gatagcaatt atgagtcgta agaaaaaaga cgatgaaacc ttaaacccaa
    27481 aggaaaatac accacctgaa gataaggttg aagagcaacc aaaaccgcct gaagaggaaa
    27541 caaaacctga tgatggtgct gtgattgacc ctatcgcctt tgaaatcaag ctgaaatcta
    27601 tccacccaca ggcaacatat ggtcgttgtg gctaccgttt taataaagaa acggcggtgg
    27661 aaattgctcg tgctgattta actgatgagc agattatcac attagctaac gacccttatt
    27721 tggagtttgt acctgttgtt gagggcgatg cgtaatgtat gcgttggttg atgatttcgt
    27781 tttgcgaatt ggcgaacagg aatccattga gctaaccgac cgtgaaatgg taggcgtggt
    27841 tgatgaagcg actcttgaag tagctttagc ggactcttcc agccaaattg atggctattt
    27901 aagtgggcgt taccgtctgc cattaaagac tgtaccgcaa aatttaaagc gtatttgctg
    27961 tgatttggct cgttatcatt taaccagcaa atcattagta acgatgaccg aagaggtgga
    28021 aaaccgctac aaactttgct taaaagagct ggaaaacatc tctaagggcg tagtgtcgtt
    28081 agcggttgat gaaaccaatg aggaagaatc ggctactggc gaaaatacag tgcagttttt
    28141 taacgggggc aaccgtattt gggggcgtga taagcgatga ttagcaaaat tgagcgtgct
    28201 ttgattgagc gtttaacgct gggcttaggc aaaatggtgt atgccgttgc tggttatgcc
    28261 ggggaaatta acgatgagaa gttagatgtt ctccgtttgc ctgcttgtct tgtctcttat
    28321 ggtggctcgg cgttcgaggc gaaatcaatg ggagtaagag gtaaacgcta tcaagccact
    28381 gatgtgtttg tggtgttggt tattactcgc tcaatgcgaa atcagactgc tgggcgttta
    28441 ggtggggtaa caagtcgtga ggtgggggtt aatcagttgc tgggagcggt gaaaacctta
    28501 ttgattaacc agactttaga cggtttagtc caccctatcc agcctaagcg aatccgcacg
    28561 atttggaata atgcggaggt gaagaaagaa aaactctctg cttatgctat tgagtttgaa
    28621 atcagctaca acgagtgcaa tgtgcttgat gacggtagat tccctgaagg ggttgagccg
    28681 aatgagcaag tctttaagca gtaccaaggc aagctagatg aaccgcaagg cgatttgtcg
    28741 ggctttaata atcggatttt tgacccgaat aacaatgccg aaacggcatt tagaacagaa
    28801 tttaaggatg gtgattagat gttaaaagtg aaagccgcta aaggggtgcg tgtgccggta
    28861 gaaaaccgac cgcatcacta tattgaacaa gaggcagtag aggttgaaaa caccctttac
    28921 tatcaacgcc gcattgcgga tggcgatttg attgtcgtca aagaaaccaa atccgctaaa
    28981 gaaacagggg gcaaataatg tcaaaaattg aatttgagaa aatcccgaac agtattcgca
    29041 aaccaagtgt gctgaccgaa tacaacaaca aagacgcagt aaccaccttg ccgaccaatg
    29101 agcaagaggt gctaattgtt gcaccaatgc taaacggtga ggctgctttt actgctccgc
    29161 aaaagatttt tagcgatgtg gaggcggaaa atttatttgg taaaggctcg gtagctcatt
    29221 taatggttcg ccaagcaatc caaaataatc cgctaatccg tttaacggtg gtgggcttaa
    29281 aagaccacga ggcaggtatt gcagcaacag ggcaagttag ttttaccggc acagtaactt
    29341 atgccggtgt ggtgcgtatt acgattgccg gcacagctta cgaaattgca gccgcaaaag
    29401 gcgaagaagc acaggcgatt gtggctcgtt tagttaatgt gattaatgcg gcgagttata
    29461 gccctgtggt ggcaagtgtg gaaagtgaaa cgacacttaa actgacctca aaagcaaaag
    29521 gcgaaatcgg caatgaaatc acgcttgcaa cacgcaatac agcaacaggt ttaacccttg
    29581 aggctcgtgc gtttgataac ggtcagcgta atgccctaat tgctccggcg ttggcgagtg
    29641 ttgccggtac gcattataac gtgattattt cgccgttttc agatgatgaa aatgcccttg
    29701 ccttgcgtag ccatttagaa tcggtttctg ccccgattga agataaaccg gctattggtg
    29761 taatgggctg gcgtggcact tatgccaccg gcacaacctt aaccgcaagc cttaacagtg
    29821 agcgaattat tgtaggctgg tataagggag caaccgaatc aaatgcaatg attgcggcag
    29881 ggcttggtgc ggtaattgcc ggtgaagaag atccggctcg cccgttaaat acccttgaag
    29941 ttaaaggctt aacggtcgta gatgactcgc agaaaccgtt atttagcgaa gtaaaccaag
    30001 cattatatca cggtttaagc ccgattgagg tggtggcaag ccgtgtgcag attacccgtg
    30061 cgattactac atacaccaaa aacgtgacga atactgatga cccaagctat ttggatttaa
    30121 ccaccattcg tacgcttgac tatgtgcgta aggcaatcca aacccgtcag cgtttgcgtt
    30181 tccctcgtgc gaaaaactcc caccgtattg tggcaaaagt acgctcggag attttagatg
    30241 tgctttatca gcttgagagc gaagaggtaa tcgaaaatgt ggaagcgtgg aaaaatcgct
    30301 tattagtggt gcgtaatcaa gacccgactt acttggattt agaaatccct gcggatgtgg
    30361 ttaatggctt gcacgttatt cgtaacaaaa ttacgttaat tttatagggg gtgctagatg
    30421 tcaattgttt atgaaggctc ttgcgtgctt gaggtagatg gcgttgagat tgaaattacc
    30481 aaacttgatg taaaaaccgt aaccggtcgc aagctggtta aaacgatgaa cagttccgga
    30541 cgtgcaagag gttattcgca aggcattgcg gaatatactc tctcggtgac tgcagttgag
    30601 cctcaagatg ggacagtgat tgattgggat aatatcaaaa atgctaagct cactaaatat
    30661 ccactggata acagtgaaaa acgtacctct tatttaggct gttttactac cgatgtgagc
    30721 gagtcttaca ccgttgatgg tgagtcggtg cgtgatattc aactcaacgc attgcgtaag
    30781 gtgaatgaat aatgaaaatc actttaaatt ttggcattga atataacggg aaaattcgcc
    30841 gtgattgtac ggtgcgattg ctgacgatgg gcggtcaatg cacggctcaa gaaatgattg
    30901 cggatttagg gtttgaggaa aactcggaag ccctatcaaa gcgtgaacaa accttggtcg
    30961 atatggctta cctttcgcag caagttacct ttgatggtat ttatcaaggt aatgtgacgg
    31021 ctcaattttt atttgacaat ttagctcccg aagactactg gcagttgatt gaggcaacgc
    31081 tcacactccg aaaaaagcgg ttagaaaatg gggtgagcca aagcgagagc gaaagcccgg
    31141 caaatcccga tttggcgtag cagaagcctt taagcagtac cgacaagcgg tgattttact
    31201 ctcaaaattt gcaattaccg ccgagcaggt atggcaaatg ccgttgcttg aggtatttgc
    31261 gtggattgag agctacttgg agagtgaggg cgtaaaaaca ccgcatagcc caatgggtgc
    31321 gaatagcgaa gtgaaacgcg aaagctatgt ctttactcgg tgtggcaaaa ttgaataggg
    31381 gcgaaagccc cttttttgta ctttaaaatt agtttaaaga gactttaaaa atggcaaaaa
    31441 atatgaaagt gcaactggaa ttgacggcaa aagatactgc ctccccagtt ttaaaccaag
    31501 ccgctaaaac caccgaaaaa gcctttaaaa atacagagcg agcggcacaa caaagtggca
    31561 atgctcaaac gcaagcagca cagaaagcag cgaatgctac agaaagctca aatcggaaaa
    31621 tagaacagag ttaccgtaat gcccgccgag tggctgccga tgtgagccga gcccgtgaaa
    31681 cattaggtgt tcgggcggaa aatgcaatcc aacgtgaaat tgaacaaact cgtgcagcct
    31741 atgaccgctt aaaacgctca ggtgttgcct cgcaaaatga gctacgccgt gcttcagagg
    31801 caacaaagaa acgtgtgcag gaattgaatg cggaactggg gaaaacgagt tttggggata
    31861 aagctggaag ttttgggcgt gggttgatgg gtgcaggggt tggaattgct gcaggtgcag
    31921 caatggctcg ccagcctgca agaaatcaaa tggaatttga tagacagctt gcaatggtag
    31981 cgaataccgc cttttcagat cgtgatgtga aagggcgtat tgatgggaaa agagagttac
    32041 ttgatgccgt acagaaagca gtaagtgtag gcggtggctc aaaagaagaa gcgttgagct
    32101 cactagacgc tttacttgcc tcaggtgctg tttctaccga aaccgctctc aatttactcc
    32161 caacgttgca gaaaggtgcg gtcgcaacag gggcaagtgc aaatgatatg gctcaaattg
    32221 ctatttcctc aatgcaacaa tttgggatta aagaagagga tattggtcga gtcttagata
    32281 tggcagttgc tgctgggcaa gccggtagct ttgagcttgc aaatatggca agttggttgc
    32341 ctcaacaaat ggccgctgca aaacaagctg gcttaagcgg cattgaggga tttgagcgtc
    32401 ttttaattgc caatcagcaa gctcgtgtca ctgccggtac aagtaacgag gctggtaata
    32461 atttagtgaa tctgttgggt aagattactg ctaaagaaac taatgagcgt tttaagaata
    32521 ttgaatataa agatccgaaa actggcaaaa ccaaagcgat tgattttgca aaagctatgg
    32581 agcattacaa gggaaaaggg caagattcat tgcaggcttt tatgtctatt atggatgatg
    32641 ttgtcgggag tgataaacgc tatcaagaat tacaagccaa actaaaaaca gctaaaggtg
    32701 aagatcagaa acgcttattc aatgaattaa ccaacttggt tgaaggtact gcaatcggag
    32761 aaattatttc tgatcgtcaa gcattaatgg ctttgctggg gataaagaat aatgttcagt
    32821 taggtgaaaa ggtacaaaaa gatattcaga atagtgaagg ggctgttgaa acctcccacg
    32881 ctgttatccg tgatactaat tcgcacaaag tggaggcatt aaagaatgcg accgaatttg
    32941 cccaaatgga gaactttgag aaagtaaacg gtgtgttggg tactttagcg gaacatttaa
    33001 cgagctatgc taaccaatac cctaatttaa ctcagtttgt tagcggagcg actgatgccg
    33061 ttaaaatctt tggggctgca ttagcagcct ctagtgtgct ggatttattg actggcaaaa
    33121 agggaaatga tggtggaatg ggtgatttat ttgataaatc atcgaaagct actggaagta
    33181 aaggaaaagt tagtaaattt ctttcagttg gaggctcatt tgctagtgtt gcgggcgttg
    33241 ccacaatgct acacggagac caagctccga tgagtgagga gcgaaaagca caattaaacg
    33301 accctaatta tcaagctcgt caaatgtacg aatataaaaa agagatttta ggtagccgtt
    33361 tccactcgtt ttttcgtagt aatgaaaccc ttgagcaata tcggaaagat gtgagtgctt
    33421 atgaggctaa gcaaaagaat ggcaatacta tgggagctgt acaaggggtt ttaagtacca
    33481 ttccgagctt acaagagtta cgccctaaag ttgattcggt tactcaaacc ctatccacct
    33541 accaagccga ctttcaggca ttcggagcaa ctctttctgc cggtattgag gcagggcttg
    33601 cctcgcaatc tcatacgatt gcaaatcaaa tcaccgtgga gttggacggt tcggtggtgg
    33661 ctgaaaaagt ggctgaatat cagtttaatt ttaacaagag gatggcgtaa tgagcggttg
    33721 gacaatgcct atacagcagg caagttttaa aggtgtgcgt ttcgatgtgc ttgcggtaga
    33781 tgacagcttt gagcgagcgg tgattgaaca cgcctaccct tttgtgaatg gggctgactt
    33841 agaggatatg gggctaaaca cccaaactat tcggctgcaa gcggtctttt tcggctcaag
    33901 ttattacacg gactatatcc gcttactgtc ggtattgcag caaaaagggg ctgatgtatt
    33961 agtccatcct attcgtgggc gtttaccgaa tatgttactc gtttcggcga atctacatac
    34021 cgatgccgaa aatatcaatt atgttgcctt agatttgact tttaaagagg caaccgaaat
    34081 gcagccgatt tttgcaatcg aagattctct gattactaaa attgataggt tgttgcttga
    34141 tctcgaaaac ctatttgatg atggattgcg tttttgggat agcgttgcgg tgtttgatct
    34201 taaatctcgc ttgcttgggc tttggggggc attatttgcc tcatttgagg cggtgcgtaa
    34261 cttgtttgat ttagacaaga aaaaatacag cattgcctca tcaacctcac aacgaaccta
    34321 tttgaaagat ggttcggggg cattacacca gttaaaagaa atggtggata gcggtattca
    34381 ggggcgtgct gatttaacga cactctcgtt taaatctcag ttactcaata ttacggatgc
    34441 ggtagataac attcaggcga ttgtgagcaa agtggctaga gatgagggct ttaaccgctc
    34501 aaaaacggtt aagttacttg gctctgatgt ggttgagctt ggtattatgc ttgaccttat
    34561 tgccacgact aagctcacta aagttttaac agaactgatt gaggatcgag aggaagagct
    34621 gctaatcggt gatttagagt taattaacca aattgtgcgt gaacattggt taaacattat
    34681 caataaactg cgtgagcaac aaaagcacgc tcaaaaaggc ataatgagtg ataagacaaa
    34741 atctatctac gagaccgcag aaaagttgat taatcgtatc cgtaatgtag ctcatcaatt
    34801 tactgtactt gt
//
LOCUS       DANKBV010000009        32532 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.009, whole genome shotgun sequence.
ACCESSION   DANKBV010000009 DANKBV010000000
VERSION     DANKBV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 32532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32532
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.009"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(51..2012)
                     /gene="recD"
                     /locus_tag="PWY43_000352"
     CDS             complement(51..2012)
                     /gene="recD"
                     /locus_tag="PWY43_000352"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDL2135267.1"
                     /translation="MLNLLLELKQEKAISDLNYQFARFIDNKQKPYGYSPLEQDLAVL
                     AAALVSYQVGLGHTALRLENLSLSSLFELNSKPQTYHLLEQILQKIEQIPPLEWQSVL
                     KNHIAFSYSPETIKPMLFQNSLLYFYRYWQAEHRIAGYLQQAVDFSEKFANTELNQSI
                     LNQLFTGKAQTIDWQKIAVATALEKQFSLISGGPGTGKTTTVVKLLLGLQLKQQALQK
                     PYLQIALAAPTGKAAARMKESLESKLAEEKGLSEELKRSIPTEAVTIHRLLGARPLTE
                     KTKYHTKNPWHYDLVVLDEASMIDLSMMEKVVQALKPNSRLIMLGDKDQLASVEAGSI
                     MGELGSFIDNGYSQSHCDYLKAVTDYQIEAGNALPIGDCLSHLKHSYRFGEKAWIGEL
                     ANAVNDQKINLSWEIFDKYKNSDKLENFCYPETKETTDKSSWIEKSVQMVVDKAVELY
                     RDYLQAVQKREKNPDSVSVKAIFEKFQKVRILSALRVSELGVERLNMAIAEGLRKASL
                     VKFNHSRDRYIGKPILMTENMPINSIASGDVGIILPDENGESRVYFDSLQENREHHSL
                     PLGRVSNYEAAYIMTVHKSQGSEFEHTVLVLPHSISPVLTKELIYTAITRAKDKFTLF
                     GSEKVWKYSVGAKTERQSGLKEQIRGRFS"
     gene            2142..3266
                     /gene="alr"
                     /locus_tag="PWY43_000353"
     CDS             2142..3266
                     /gene="alr"
                     /locus_tag="PWY43_000353"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL2135268.1"
                     /translation="MKPATATIDSQALRHNIRLIKSFAPQQKLLAMIKANAYGQGLLP
                     AAGTLADMVDGFGVARLREALEIQETGYTGKIVLVEGFFDREELLKTLSRKFDTVIHN
                     LEQLELLEQVAKEWQAEQQKGFWTRKAKIYFPINIWLKIDTGMHRLGIHPEQVDHFVE
                     RLKNCALVNSISFVSHFSRADELDCGYTEKQIAVFEQATQKYQDHDRSISASSGILYW
                     KQAHYDWVRPGIIMHGISPHYTPITDLGFKPVMTLSSSLIAVRTHKAGEPVGYGGAWV
                     SPKDTKLGVIAMGYGDGYPRNAPEGTPVLINGRIVPIVGRVSMDMLTVDLGAESQDKV
                     GDKAIFWGEQLLIEDVAKHIGVISYELITKLTPRVIFEYK"
     gene            complement(3329..3970)
                     /gene="sodA"
                     /locus_tag="PWY43_000354"
     CDS             complement(3329..3970)
                     /gene="sodA"
                     /locus_tag="PWY43_000354"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDL2135269.1"
                     /translation="MAYTLPELGYAYDALEPHFDAKTMEIHHSKHHQAYINNANAALE
                     AHPELLEKCPGALIKDLSQVPAEKRIAVRNNVGGHVNHTLFWKGLKTGTTLQGALKEA
                     IERDFGSVEAFQSEFEKAATTRVGSGWAWLVLEEGKLAVVSTANQDNPLMGKEVAGVS
                     GYPILVLDVWEHAYYLNYQNRRPDFIKAFWNVVNWDEAARRFEEKVATCGCAK"
     gene            4414..6870
                     /gene="thrA"
                     /locus_tag="PWY43_000355"
     CDS             4414..6870
                     /gene="thrA"
                     /locus_tag="PWY43_000355"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDL2135270.1"
                     /translation="MRVLKFGGTSLANPERFMQAAEIIEKAHLADQAAGVLSAPAKIT
                     NHLVAIVDKAIAGESFQSNLSEATEIFHNIINGLYATNNHFAKEALLALVEAELNQIQ
                     QVAAEAAQTKSLSDKIAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
                     LESSVDIAESTKRVDAGSIPKKNIVLMAGFTAGNEQGELVLLGRNGSDYSAACLAACL
                     KADVCEIWTDVDGVYTCDPRLVPDAVCLETMSYQEAMELSYFGAKVIHPRTIGPLVPL
                     NIPCLIKNTGNPDGKGTLIDGNVANDNLKVKGITNLDNVAMFNVSGSGMQGMVGMAAR
                     VFSTMSKAGISVILITQSSSEYSISFCVPAKSAEKALAALNTEFAQELSENLLDPIDI
                     IKDLSIVSVVGDGMRTAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPLNKAIE
                     AVKATHKSLFNSKRVVDMFLVGVGGVGSELIEQVKQQKEFLAKKDIEIRVCALANSDK
                     MLLNENGLNLDNWKEDLEKAIQPSDFDVLLSFIKFHHVVNPVFVDCTSAQSVANLYAR
                     ALKEGFHVVTPNKKANTSGYDYYLEMRENARQSQHKFLYETNVGAGLPVIENLQNLLA
                     AGDEVEKFEGILSGSLSFIFGKLEEGLTLSQATLIAKEKGFTEPDPRDDLSGADVARK
                     LLILVRETGLPLEFEDIEIEGVLPKGFSEGMSRDEFLQALPSLDEEFSKRVEAAKAEG
                     KVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYNPIPLLLRGYGAGN
                     SVTAAGIFADILRTLQGGVN"
     gene            6870..7817
                     /gene="thrB"
                     /locus_tag="PWY43_000356"
     CDS             6870..7817
                     /gene="thrB"
                     /locus_tag="PWY43_000356"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDL2135271.1"
                     /translation="MALRIYAPASSANISVGFDSLGAAISPIDGSLLGDVVQIEESEQ
                     QFELESAGYFVRKLPKEPQKNIVYQAYVLFSERLKLRGGNVKNLRLTLEKNMPIGSGL
                     GSSACSIVAALVALNKFHDEQFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGLQLM
                     TQSLGNICQPLPFFDEWYWVLAYPGVEVSTAEARAILPKSYTRQDMIQQARYLGSFVH
                     ACHTHQDILAATMMKDLIAEPYREDLLPNFPAVRQGCKDLGALAVGISGSGPTMFAIA
                     PDLEHAQRLVSYLEKEYLQNNEGFIHICKVDNQGARELK"
     gene            complement(7932..9311)
                     /gene="cysS"
                     /locus_tag="PWY43_000357"
     CDS             complement(7932..9311)
                     /gene="cysS"
                     /locus_tag="PWY43_000357"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL2135272.1"
                     /translation="MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTF
                     VSFDVIARYLRYSGYNLRYVRNITDVDDKIIKRALENNETCDELVDRMIAEMHKDFDA
                     LNILRPDVEPRATKHIPEIIAMVEKLIHNGHAYVAADGDVMFDVESFQKYGALSRQNL
                     EQLQAGARVEIKSIKKNPMDFVLWKMSKAGEPSWESPWGNGRPGWHIECSAMNSKELG
                     EHFDIHGGGSDLMFPHHENEIAQSCCAHGGDYVNYWLHTGMLTIDKEKMSKSLGNFFS
                     IRHMLGLYDAESLRYFFLTAHYRSLLDYSIDNLDLARSALERLYTALRGCDVSGEAIG
                     GEHYVEAFKTAMDDDFNTPGALAVLFEIARELNKLKTEDMAKANGLAVRLKELAGVLG
                     LLEQDPDAFLKGDANDDETAEIEALIKQRNEAKAAKNWAVADEVRDKLKAINIVLEDT
                     PNGTIWRKA"
     gene            9467..9976
                     /locus_tag="PWY43_000358"
     CDS             9467..9976
                     /locus_tag="PWY43_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2135273.1"
                     /translation="MITLHTNFGDIKIELNFEKAPITAKNFEDYAKEGFYNGTIFHRV
                     IDGFMIQGGGMTAGMAEKPTKDPIQNEANNGLSNKRGTIAMARTSDPHSASSQFFINV
                     ADNTFLDYRSKEMFGRSVVQDWGYAVFGEVVEGMDVVDKIKGVKTGNKGFHQDVPVED
                     VVIESVSVA"
     gene            complement(10068..10995)
                     /locus_tag="PWY43_000359"
                     /pseudo
     CDS             complement(10068..10995)
                     /locus_tag="PWY43_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
     gene            complement(10998..11792)
                     /gene="fdhD"
                     /locus_tag="PWY43_000360"
     CDS             complement(10998..11792)
                     /gene="fdhD"
                     /locus_tag="PWY43_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDL2135274.1"
                     /translation="MNAKKLSLSTNSIKQKDDEENLISEVPVALVYNGISHTVMMATP
                     ANLEDFALGFSLAEGILNAPSELYGLDVIEQENGVEIQLEISSRQFVALKDKRRSMAG
                     RTGCGICGVEQLEQVNQSCKIVKKNNRLQNVNPLILEDCLLQLENAQFLSKETGASHA
                     AAFFTPDGKLLAIREDVGRHVALDKLIGWYAKADFPIGFVFVTSRASFEMVQKCLAVG
                     IEMLCAISATTEMAVNMARENQFTLLAFARKGKATIYAGEERLALN"
     gene            12046..15117
                     /gene="fdnG"
                     /locus_tag="PWY43_000361"
     CDS             12046..15117
                     /gene="fdnG"
                     /locus_tag="PWY43_000361"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12631..12633,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDL2135275.1"
                     /translation="MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAK
                     ETRQTCTYCAVGCGMIMYSLGGEAMNSKGKLIHIEGDSDHPVSRGALCPKGAGALDYV
                     NSDRRVHYPEVREAGSSEWKRISWHEAIERIARHMKNDRDENFVTHNEAGLEVNRWMT
                     AGFLAGSACSNETGIVTQKFIRSLGMIFTDNQASIUHGPTVASLAPSFGRGAMTNHWV
                     DIKNSDLVIVMGGNAAEAHPVGFRWAVEAKKQNGAKLMVVDPRFNRSAAVADIYIPLR
                     PGTDIAFLLGAVNYLLKNDKIQHEYVKHYTNATFLLNEGFKFEDGLFTGYDETTRTYK
                     DKSTWAYQLDEQGQPKRDMTMQDPRCVINMLKDHVSRYTPEMVERITGTPQKDFLIFC
                     EEIAKTSAPDKAATFLYALGWTQHTVGAQNIRSMAIIQLLCGNIGVSGGGVNALRGHS
                     NVQGITDLGLFPHMLPGYIRLPTEADATLESYLNAIAPKTLVAGQVNYGQNNPKFAVS
                     MLKTFYGDKATKENEFGYHYLPKYPKGGTDHFRFIEQMYQGKVNGYFCQGMNPIASYP
                     NSQKLIKALSNLKYLVIFDPLITDTSEFWKNHGEFNNVNPSEIQTEVFRLPTTCFAEE
                     DGSIANSGRWLQWHWKAADAPHEAKPDVDILSEIRAELIHLYHKEGGKAPLEPLEAMT
                     WNYMNPLEPKPEEIAKENNGYALEDLKDADGNIIVKKGELLSSFAQMRDDGTTSGGCW
                     IYCGQWTPKGNQMANRDNSDPSGLGNTLGWAFSWPVNRRILYNRASADLSGKPWNPKR
                     QLVKWNGKNWNYVDVADYGTAPPNSPVTPFIMQPEGVSGLFVRERMQDGPFPEHYEPF
                     ETPIGTNPLHPNVISSPVARILPSDKADLGTSDEFPYVGTTYRLTEHFHYWTKNALIN
                     VIAQPEQFVEIGEKLAEEKGIKNGDKVRVWSKRGEIKAVAVVTKRIRSLVSDGKPIHT
                     IGVPIHWGFAATTGAKKGYFANNLTVRTGEPNSNTPESKCMLLNIEKIVGA"
     gene            15120..16034
                     /gene="fdxH"
                     /locus_tag="PWY43_000362"
     CDS             15120..16034
                     /gene="fdxH"
                     /locus_tag="PWY43_000362"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HDL2135276.1"
                     /translation="MSTVQTQNIIKISATSALTPAPQARSHQVEVAKLIDVTTCIGCK
                     ACQVGCSEWNDIRSDVNANCVGVYDNPADLNAKAWTVMRFNEVEENDRLEWLIRKDGC
                     MHCAEPGCLKSCPSPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPDDNRVY
                     KCTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMKFYAEQRIADLKSRGYENAGLYDP
                     EGVGGTHVMYILHHADKPELYSGLPKDPKIDTTVTLWKDVLKPVAAVALGGLALAEVA
                     HYITVGPNVEEDLEEHHHSENAEGGKHE"
     gene            16027..16704
                     /locus_tag="PWY43_000363"
     CDS             16027..16704
                     /locus_tag="PWY43_000363"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDL2135277.1"
                     /translation="MSKKFEITNDTKIVRHKPLARVSHWFLVISFFMTMFTGVAFFFP
                     DFAWLTEILGTPQIARAVHPFTGIIMFIAFIIMALIYWHHNIPEKNDIRWAKGIVEVL
                     KGNEHKVSDNGKYNLGQKLLFWTLILAMFTLLATGIIMWRQFFSHYFSIPMLRIAILL
                     HSASAFMLFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELERQ
                     VAEQNNK"
     gene            16760..17677
                     /gene="fdhE"
                     /locus_tag="PWY43_000364"
     CDS             16760..17677
                     /gene="fdhE"
                     /locus_tag="PWY43_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDL2135278.1"
                     /translation="MSIKILPQDDIKQAASSFQQPELLFANPKNLYLRRAKRLRELAK
                     QNPFGEYMEFSANLVDIQLALLESQPIADYSQKITACVERTQGETPLNAQNFQRTDEW
                     RTLLLAIIEKFKPYANDTVLATIEVLEKAAKSELDTLADNLLNERYELVGADKAVFIW
                     AALSLYWVQLAQQLPRNSRAEIGHKHLCPVCNSAPVSSVIHFGEAQGLRYLHCSLCES
                     EWHVVRTKCTNCDETGKLDYWSLDTVEAAVKAESCGDCHSYLKVLYQDKDVNVEPVAD
                     DLATLFLDSEMEQKGLSRSGINPFLFQVE"
     gene            complement(17747..18133)
                     /locus_tag="PWY43_000365"
     CDS             complement(17747..18133)
                     /locus_tag="PWY43_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135279.1"
                     /translation="MLRNFMIMLHFIYGFAMKNAFSFFVISLFLTMNSISYASNPIPS
                     NKEKLNGVKKQQDKPKFRISERKVLKNKEKDEEVSNFSMEELRVGAQRPLDDSVIQYF
                     KSQCRYAFMSDREIIANRCEVKKIAD"
     gene            complement(18144..19514)
                     /gene="gorA"
                     /locus_tag="PWY43_000366"
     CDS             complement(18144..19514)
                     /gene="gorA"
                     /locus_tag="PWY43_000366"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL2135280.1"
                     /translation="MAKHYDYIAIGGGSGGIASINRAASYGQKCAIIEAKHLGGTCVN
                     VGCVPKKVMFYGAQVAEAINHYAPDYGFDITVNNFDYAKLVESRQAYIGRIHTSYGNV
                     LAKNNVDVLNGFARFKDAKTLEVSYADGSVEEVTADHILIATGGRPTVPNVKGAEYGI
                     TSDGVFALDALPKSVAIVGAGYIAVELAGVFNSLGVNTHLFVRQHAPMRNQDPLIVDT
                     LLEVLAQDGIELHTQATPQEVVKNADGSLTLKLQDGRETSVESLVWAIGREPATDKIN
                     LEAAGVETNERGFIKVDKFQNTNVPNIYAVGDIIEGGIELTPVAVAAGRRLSERLFNN
                     KPNEHLDYSLVPTVVFSHPPIGTVGLSEPKAIEQYGAENVKVYKSSFTAMYTAVTQHR
                     QPCRMKLVCVGEEEKVVGLHGIGFGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSE
                     EFVTMR"
     gene            complement(19591..20115)
                     /locus_tag="PWY43_000367"
     CDS             complement(19591..20115)
                     /locus_tag="PWY43_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDL2135281.1"
                     /translation="MHSELNTRANILGKIIFASRWLQLPIYLGLIVVQGIYAYKFIKS
                     LYHLVINLNEMDSNTVMLAVLNLIDVVMIANLLVMVIVGGYETFVSRLKVDDHPDQPE
                     WLDHVNATILKVKLSMAIISISSIHLLQTFINASNLDEKQMMWQVVIHLAFIVSALAM
                     AYIDKMLNSGHMKH"
     gene            complement(20210..20848)
                     /locus_tag="PWY43_000368"
     CDS             complement(20210..20848)
                     /locus_tag="PWY43_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2135282.1"
                     /translation="MELKHTIEQRKTIKAFNSTIKISRTELEEMVALSQLAPSKANLQ
                     PWRFVIIDDEILKAALSKNVAFNGPPCETASAIVVILADLQYEKLLGAIVDQSVETGC
                     LHKEFRERKFDFLLNLHNQATQAEIRDQVLIDTSLAAMQLMLIAKDKGYDTHAIGVFE
                     RPAVLKLLNIDAERYLPVMLLAIGKAANPALPSCRLPLEYTVSWNDGQGFKI"
     gene            complement(20990..21784)
                     /locus_tag="PWY43_000369"
     CDS             complement(20990..21784)
                     /locus_tag="PWY43_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2135283.1"
                     /translation="MALLEVNHLNKRFVERSSLFHKQDFYAVKEVSFTLNRKETLAII
                     GENGAGKSTLAKMVAGMVEPTSGELIFRGTPLSFGDYKYRAKHIRMMFQDPNDAFDPN
                     YNIGQILDAPLRLATDLNEEERNERIFNTLKLVGMYPEQALVPISEASSGQKQRIAFA
                     RALILEPEIIIADDTINSLDFSVKTQLINLMLSVQQRQGIAYIYVGQNLGLIKHIADK
                     IMVMQSGEVVEYGLTKEVLLNPQHQITKRLIESHFGKHLSSYAWAS"
     gene            complement(21885..22961)
                     /gene="fbaA"
                     /locus_tag="PWY43_000370"
     CDS             complement(21885..22961)
                     /gene="fbaA"
                     /locus_tag="PWY43_000370"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDL2135284.1"
                     /translation="MSLLNIVKPGVVTGDDVQKVFAYAKEHNFAIPAVNCVGSDSVNA
                     VLETAARVKSPVIVQFSNGGAQFYAGKGIKPASGARADVLGAIAGAKHVHQLAEEYGV
                     PVILHTDHAAKNLLPWIDGLLEAGEKHFAETGRPLFSSHMIDLSEEPIEENMEICREY
                     LARMDKIGMTLEIEIGVTGGEEDGVDNSDVDESKLYTQPSEVLYVYDQLSPISPRFTI
                     AAAFGNVHGVYKPGNVKLKPSILGASQEYVSKERGLPAKSLDFVFHGGSGSSREEIRE
                     AISYGAIKMNIDTDTQWAAWDGILQFYKANEAYLQGQLGNPTGPDAPNKKYYDPRVWL
                     RKMEESMSKRLEQSFEDLNCVNVL"
     gene            complement(23058..24233)
                     /locus_tag="PWY43_000371"
     CDS             complement(23058..24233)
                     /locus_tag="PWY43_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL2135285.1"
                     /translation="MSVIKMTDLDLAGKRLFIRADLNVPVKDGKVTSDARIVATIPTL
                     KLALQKGAKVMVTSHLGRPTEGVFEEENSLQPVVDYLNASDLGVPVRLVRDYLDGVEV
                     NENEIVVLENVRINKGEKKNDPELAKKYASLCDVFVMDAFGTAHRAEGSTYGVAEYAP
                     VACAGPLLAAELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGI
                     ANTFIAAEGHAVGKSLYEEDLIPEAKRLAQVTNIPVPVDVRVGTEFSETAPATEKAVS
                     EVRADESIFDIGEKSAEQLAEIIRNSKTILWNGPVGVFEFPNFRKGTEVISNAIAEAT
                     ANGAFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG"
     gene            complement(24489..25847)
                     /locus_tag="PWY43_000372"
     CDS             complement(24489..25847)
                     /locus_tag="PWY43_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL2135286.1"
                     /translation="MANQQQERQSWSNRLAYIMTVAGATVGFGATWRFPYLVGEHGGG
                     AYVFLFCIAMIVIGIPMILVENVIGRRMRVNAIDAFAGFANGKKIASGWKILGYMGLL
                     GAFGILAYYMVLGGWVLNYIGGLLTGTLDLSQPVTVGTTQQFFQQSIFNSPMTIIAYT
                     ALFVLVNYFILVKGIVDGIERSVKYLMPILFIFLIVMVVRNITLPGAAEGIAFYLMPD
                     FSKITPKLFVFVLGQVFFALSLGFGVLITLSSYLDKSENLVKTATITGVMNTLIAVLA
                     GFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSNMWGGTVFAVIFFSLLVVAALTTSLTI
                     YEVIITALQEKAKISRQKAIFITLATIFLLGNIPSALSDNILQDVQVMGKSIFDAFDY
                     ISGNILFILTALGSAIFVGFVLKEEAKEELGAGKMFTTIWFNYVRFVIPVIILVIFFN
                     SL"
     gene            complement(25917..26744)
                     /locus_tag="PWY43_000373"
     CDS             complement(25917..26744)
                     /locus_tag="PWY43_000373"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDL2135287.1"
                     /translation="MNHPKHRAFLKWAGGKYRLIPDIQTHLPKKACLVEPFVGAGSVF
                     LNTDFERYILADINPDLINLFNVVKADVEEYIAQTKQLFLHPQANTEAFYKACRAEFN
                     RSKDIFRRSVIFLYLNRFGYNGLCRYNQKKGYNVPFGRYKTHYFPEKELRFFAEKAQK
                     ATFVCADFEQVFELAKNQLTDYVIYCDPPYAPLAQETNFTQYAGGGFSLEQQGRLATL
                     ALEMKGLGVPVLISNHDTDFTRRIYQSADITQIQVQRSIGQKVGSRVKVGELFALFI"
     gene            complement(26758..27846)
                     /gene="aroB"
                     /locus_tag="PWY43_000374"
     CDS             complement(26758..27846)
                     /gene="aroB"
                     /locus_tag="PWY43_000374"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL2135288.1"
                     /translation="MLQVNVELKERRYPIIIGAGLLNQPASYSPLKSGDKVMIVSNPT
                     VATHYLSVVTNALKELGCHVDSVLIPDGEEYKTLESLNLIFTALLEKNHNRDTTLIAL
                     GGGVIGDVAGYAAASYQRGIRFIQIPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     VSVIVDTNTLHTLSKREVSAGLAEVIKYGAIFDVTFFEWLEKHIDDLVSLKQDELEYC
                     IQRCCQLKADVVARDETEKGDRALLNLGHTFGHAIEARMGYGVWLHGEAVSVGMLEAA
                     ELSRILGDLSPQEVARLEKLLARANLPTISPDGMEPAEYLPYMWRDKKVLAGQLRLIL
                     LKSLGEAYVSSQATESQVLASIERFTQR"
     gene            complement(27870..28391)
                     /gene="aroK"
                     /locus_tag="PWY43_000375"
     CDS             complement(27870..28391)
                     /gene="aroK"
                     /locus_tag="PWY43_000375"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDL2135289.1"
                     /translation="MAEKRNIFLIGPMGAGKSTIGRQLAQTLGMEFMDSDSVIEERAG
                     ADIDWIFDVEGEEGFRKREERILNELTQNYGIVLSTGGGSILSKDNRNVLSARGIVIY
                     LETTVDKQFERTQRDKKRPLLQTEDPRKTLEELAKIRNPLYEEIADITIHTDEQSAKV
                     VANQIIDMIDNLQ"
     gene            complement(28628..31192)
                     /locus_tag="PWY43_000376"
     CDS             complement(28628..31192)
                     /locus_tag="PWY43_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HDL2135290.1"
                     /translation="MRIAKIIFSALLSLLVLGGVIGGLLYMHFKSDLPDVATLKNVEL
                     QQPMQIFTLDGKLIGEVGEERRIPVKLDDIPPMLVKAILATEDARFYEHKGIDPKGIM
                     RAIWRTSQGDTQGASTITQQLAKNFFLTPERSLERKAKEAILALEIEQVLSKNEILEL
                     YLNKIYLGYRSHGVAAAAKTYFNKALKDLTLSEIAIIAGLPKAPSTMNPLYSVKRAED
                     RRNVVLGRMLETGDITKEEYEKAKAEPVVANYYGATLEFRADYVTEMVRQEIVKRYGE
                     NIAYNKGLKVYATVLSADQKAAQDALRENLIDYDRRHGWRGAEKLWTGSSAWDDEKII
                     DHLSKLPKSEPFTPAVVMQADKSKYTILLANGDISVLKRTNASFAPKGLKAGEQIWVR
                     QNKNREWALGQIPEVNSALVSINSENGAIESIVGGFSFEQSRFNRATQSLVQVGSAIK
                     PFIYTAAMNKGLSLSTTISDSPIVIKKKGQKEWRPKNADGVYGGPLRTRVALGKSKNM
                     VAIRILQMAGIDYVADYLQRFGFNRNQYVATESLALGAASFTPLEMARAYAVFNNGGY
                     LIEPYIIDRIIDAQGYELYQANPVVACNSCDSPVVYPEPKYFESVKIHDEAQVGVQTI
                     ANSSNNDDDDVVEDVGDSEPELQANNSEKNAPSLMAESSHHVKGLRYAPHVISDELAF
                     LMRSALATAITGEPEYGWRGTSYKMLNTIKRADVGGKTGTTNNAKATWYAGFGANIST
                     VVYVGFDDNKRVLGRGASGSSTALPAWTNYMKVALSDKPVEKSTLPKNIIEVNIDPNS
                     GFLGNGRKEYFIKGTEPTKRYIVERAYTEKAPQKTESAPLRLGLPPPGVLKSGELF"
     gene            31316..32014
                     /locus_tag="PWY43_000377"
     CDS             31316..32014
                     /locus_tag="PWY43_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HDL2135291.1"
                     /translation="MKLLKAHSKTPSNRIILGLSEDEQRYCLVKNKAEKFTVFWQAKP
                     ASIEELIGNLTPQEQAMMTLVRPIPYQYIWRKTVFMANTLNEAQLHRKVIHILKNEQP
                     LALDLLNIDYQRFPSSNDNLDKVVIYAVRKSYTENLSQFNSILDCELHCYARAIIHLT
                     QSQNNQPAPCFSFKQKYFQFTETELLILQTEPEDCIHLSDLKIDHLEIECEQEKQLYC
                     LALGASLWNGKVLI"
     gene            31990..32511
                     /locus_tag="PWY43_000378"
     CDS             31990..32511
                     /locus_tag="PWY43_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135292.1"
                     /translation="MEWQGIDLSANRKNNWFAGSDKGIKNVIYTLLLSALISLALIFL
                     YLQKNQEIQPLAQQLELVKTDILNIENKIISLNSNHTNTLPFAEPKKIAQLLAIIDNL
                     PLKNGGIETIQIYLETDLYLKIIGNLDNQSDFQSLESYLYQQDLFETKTEQINVNHKN
                     EIQFIFTLKHKGN"
BASE COUNT         9823 a         7019 c         6185 g         9505 t
ORIGIN      
        1 tttgaaaaaa atgaccgctt gcgtattttt acggcaaaac gagtattaat ttagctaaac
       61 ctgcctctta tttgttcttt tagcccactt tgtctttcgg ttttggcacc aacactgtat
      121 ttccaaactt tttcgctacc aaataaggta aatttatctt ttgcccttgt gattgcggtg
      181 taaatcaact ctttcgtcag caccggggaa atcgaatggg gcaacaccaa taccgtatgt
      241 tcaaattcag aaccttgcga tttatgcaca gtcataatat aagctgcttc ataatttgaa
      301 accctaccta gaggcaaact atggtgttct ctattttcct gtaaggaatc aaaataaact
      361 cggctttctc cattttcgtc cggcaaaatg atgccgacat caccgctggc tatgctattt
      421 atcggcatat tttctgtcat taaaatcggt tttccgatat agcggtctcg gctatgatta
      481 aatttcacta agctagcttt ccgcaagcct tccgcaatcg ccatattaag acgttcaacc
      541 cccagttcac ttacccttaa ggctgataaa attcggactt tttgaaattt ctcaaaaatc
      601 gctttcacac tcactgaatc tggatttttc tcccgttttt gaaccgcttg taaataatca
      661 cgataaagtt caaccgcttt atccaccacc atttgcacac ttttttcaat ccagcttgat
      721 ttatccgttg tctctttcgt ttccggataa cagaaatttt ctaacttatc actgttttta
      781 tatttgtcga aaatctccca cgaaaggtta attttttgat catttactgc atttgcaagc
      841 tctccaatcc aggctttttc gccaaatcga tagctatgtt ttaagtgaga taaacaatca
      901 ccaatcggca acgcattacc ggcttcaatt tggtagtcgg taacggcttt taagtaatcg
      961 caatgggatt ggctataacc attatcaata aaagagccaa gttcccccat aattgaacca
     1021 gcttccacag aagcaagctg atctttatct cccagcatta tcaagcgaga gtttggtttg
     1081 agcgcttgaa caactttctc catcattgat aaatcaatca ttgaagcctc atctaatacg
     1141 accaaatcat agtgccacgg atttttggta tgatatttgg ttttctcagt taatggtctc
     1201 gcaccaagca agcgatgaat agtcactgct tcagttggaa tagagcgttt taactcctca
     1261 gataacccct tttcttccgc caacttgctt tccagcgact ctttcattct tgctgctgct
     1321 tttccggttg gtgcagccag agcgatttgc aaatatggtt tctgtagtgc ttgctgtttt
     1381 aattgcagcc cgagtaataa ttttactacc gtagttgttt ttccggttcc cggcccacct
     1441 gaaattaagc taaactgctt ttctaatgct gttgcaacgg caatcttttg ccagtcgata
     1501 gtttgagctt tcccagtgaa tagttggttg agaatgcttt ggtttaattc ggtatttgca
     1561 aatttttccg aaaaatcaac cgcttgttgt aaataaccgg caatgcgatg ctccgcttgc
     1621 cagtagcgat aaaaatagag caagctgttt tggaataaca tcggtttaat tgtttcggga
     1681 gaatagctaa aagcgatatg atttttcagc acactctgcc attcaagcgg tggaatttgc
     1741 tcaatttttt gcaaaatttg ttcgagcaaa tgataagttt gtggcttgct atttaattca
     1801 aaaagagaag aaagactgag attttccaaa cgtaatgccg tatgcccaag tcccacttgg
     1861 taagaaacca acgcagcggc taacacagct aaatcttgct ccaaaggcga atacccatag
     1921 ggtttctgct tattatcaat aaaacgggca aattgataat ttaagtcaga aatcgccttt
     1981 tcttgtttaa gttccaataa taaattcagc ataatcaaac cttaaatcga ccacctaaca
     2041 ttaagtgata atactaatct tttttgcatt attttctaag ggatttttta ttttaggcgt
     2101 ataatttagc ctttcttgtg attttaacta tgtagataag tatgaaacca gcaacagcaa
     2161 ccattgatag tcaagccctc cgccataata ttcgcttgat caaatctttc gctccacagc
     2221 aaaaattatt ggcgatgatc aaagccaatg cttacgggca aggcttattg ccggctgccg
     2281 gcacattagc agatatggtt gatggctttg gagttgcccg tttgcgtgaa gccttagaaa
     2341 ttcaagaaac cggatacaca ggcaaaattg tgttagttga aggttttttt gaccgagaag
     2401 agctactcaa aaccctttcc cgcaagtttg ataccgtcat tcataacctc gaacaactgg
     2461 aattactcga gcaagtagca aaagaatggc aggcagagca acaaaaaggc ttttggactc
     2521 gtaaagcgaa aatctacttc ccgattaaca tttggctaaa aatcgacacc ggaatgcacc
     2581 gcttaggcat tcacccggag caagtcgatc actttgttga acgtttgaaa aattgtgcct
     2641 tggtcaattc aatcagcttt gtgagtcatt ttagccgtgc cgatgaactc gattgtggct
     2701 ataccgaaaa acaaattgcg gtgtttgagc aagctacgca aaaatatcag gatcacgacc
     2761 gcagtatttc cgcctcaagc ggtattcttt attggaaaca ggctcattat gattgggttc
     2821 gcccgggtat tattatgcac ggcatttctc cccactatac gccgattacc gatttaggtt
     2881 ttaagccggt aatgacgctc tcctcttccc tgatcgctgt gcgtacccat aaagccggcg
     2941 agcctgtagg ctatggtggt gcttgggtaa gccctaaaga taccaaactc ggcgtgattg
     3001 caatgggcta cggcgatggc taccctcgca atgctcctga aggtacccct gtgttgatta
     3061 acggtagaat agtgccgatt gtcggtcgag tttcgatgga tatgctgacg gtcgatttag
     3121 gtgcagagag ccaagataaa gtgggcgata aagccatttt ttggggagag caactattaa
     3181 ttgaagatgt ggcgaaacac attggcgtga ttagctatga gctgattact aaactcactc
     3241 ctcgtgtgat ttttgaatat aaatagacaa caagcggtaa aattttcaac aaattttacc
     3301 gcttgtatct tattcaagat cgaaaatatt atttcgcaca accgcaggtt gctacttttt
     3361 cttcaaaacg gcgagcggct tcgtcccagt ttacaacgtt ccagaatgct ttaatgaaat
     3421 ccggacggcg gttttggtag tttaagtagt aagcgtgttc ccaaacgtct aagactaaaa
     3481 tcggatagcc tgaaacgcct gccacttctt tacccattag tgggttgtcc tggttagcgg
     3541 ttgaaacaac ggctaattta ccctcttcta atactaacca cgcccagcct gaaccaacac
     3601 gagtagtcgc tgctttttca aattctgatt ggaaagcttc aactgaaccg aaatcacgct
     3661 caatcgcttc ttttaatgca ccttgtaagg tagtgcctgt ttttaaacct ttccagaata
     3721 aagtgtggtt tacatgacca cctacgttgt tacgcactgc gatacgtttt tctgcaggca
     3781 cttggctcaa atctttgatt aatgcacccg gacatttttc taataattca gggtgagctt
     3841 ctaatgctgc atttgcattg ttgatgtaag cttggtgatg tttagaatgg tggatttcca
     3901 ttgttttagc atcgaaatgt ggctctaacg catcataagc gtagcctaat tctggtaatg
     3961 tatatgccat agttaattcc tctcaaatag taaataggtt aatcagagtt aaactccgag
     4021 tacattttat caagaaacac acaaaaaatg cacatttttt tgatctagaa caaaattatt
     4081 tcactatcta atttagaaag gttcctaatc aaattcttgc ttttaattgt tttatataga
     4141 aagacaaaga aatttaaaaa aatgtggtga aaaacagcgt taagcccatt gacaaaggca
     4201 ttattttcac gcattcttga accattattc actctcttga ccgagattcc gccaatgaat
     4261 tgttttaata tgatgacaac gattattacc accattatgt caccaacata gtgcgggttt
     4321 taattggctg aaataatgaa agaataaacc gaaaacccgc tacaagcggg tttttttgta
     4381 taaaaattta caaataattt atcaggagta actatgcgag ttcttaaatt tggaggcact
     4441 tctcttgcca accctgaacg ctttatgcaa gcggcagaga ttatcgagaa agctcatttg
     4501 gcagatcaag ctgccggcgt attatccgcc ccagccaaaa ttaccaatca tcttgtcgct
     4561 attgtagata aagcgattgc cggtgaatca tttcaatcta acctttccga agctaccgag
     4621 attttccaca acatcattaa cggcttatat gctacaaata atcactttgc taaagaagca
     4681 ctgttagcct tagtggaagc ggaattaaac caaattcagc aagtggcagc agaagcagct
     4741 caaactaaat cgttgtcaga taaaattgct gccactattc actgccgtgg tgaaaaactc
     4801 tctattgcga tgatgcaagc gtggttcgag gcaaaaggct atgaagtcac tcgtatcgac
     4861 cctgttgaaa aattattggc tcacggcagc tatttggaat cttcggtcga tattgccgaa
     4921 tccaccaagc gagttgatgc aggttcaatt ccaaagaaaa atatcgtgct aatggctggt
     4981 tttaccgcag gtaatgagca aggcgaactg gtgttattag ggcgtaacgg ttcagattat
     5041 tctgccgctt gtttggcagc gtgtttaaaa gccgatgttt gtgaaatctg gacggacgta
     5101 gatggcgttt acacctgcga ccctcgtttg gtgcctgatg cagtatgctt agaaacaatg
     5161 agctatcaag aggcaatgga gctttcctac tttggggcga aagtgatcca ccctcgcacc
     5221 atcgggccat tagtgccgct caatattcct tgtttaatca aaaataccgg caatccggac
     5281 ggaaaaggca cattaattga tggcaatgtg gcgaacgata atctcaaagt aaaagggatt
     5341 accaaccttg ataacgtggc gatgttcaac gtttcaggtt caggaatgca gggaatggtg
     5401 ggcatggctg cccgtgtgtt ctcgacgatg tcaaaagccg gtatttcggt gattttaatt
     5461 acccaatcat catcagaata tagcatcagc ttctgcgtgc cggcaaaatc ggcagaaaaa
     5521 gcgttagcgg ctctaaatac tgaatttgca caagagttat ctgaaaactt acttgacccg
     5581 attgatatta ttaaagattt atccattgtt tcggtggtgg gtgatggtat gcgtaccgcc
     5641 aaaggaattg cggctcgctt cttctctgcc ctagcccaag ccaacatcag tattgtggca
     5701 attgcacagg gttcatctga gcgttccatt tctgctgtcg tgccgttaaa caaagcgatt
     5761 gaagcagtaa aagcaactca caaatcgcta tttaacagta aacgtgtggt ggatatgttt
     5821 ttagtgggtg taggcggtgt gggtagcgaa ctcatcgagc aagtcaaaca acaaaaggag
     5881 tttttggcaa agaaggatat tgagatccgt gtgtgtgctt tagctaattc tgataaaatg
     5941 ttactcaacg aaaacggctt aaatttagac aactggaaag aagatttaga aaaagcaatt
     6001 cagccgtcag actttgatgt attgctttct ttcatcaaat tccaccacgt ggtaaaccca
     6061 gtatttgtag attgcacttc ggcacaatcg gtggcgaacc tgtacgcacg agccttaaaa
     6121 gagggcttcc acgtggtaac accaaacaaa aaagccaata catcaggtta cgattactac
     6181 ttagaaatgc gtgaaaacgc ccgccaaagc cagcacaaat tcctctacga aactaatgta
     6241 ggggcaggtt taccggtaat tgaaaactta caaaacctat tagctgccgg tgacgaagta
     6301 gagaaatttg aagggatttt atccggttcg ctctctttca ttttcggcaa attagaagaa
     6361 ggtttaaccc tttcgcaagc aactttaatc gccaaagaaa aaggctttac cgagccagat
     6421 ccacgagatg atttatccgg tgcagatgtt gctcgtaaac tcttaattct tgttcgtgaa
     6481 accggcttac cgcttgaatt tgaagatatt gaaattgaag gcgtattgcc aaaaggcttc
     6541 tcggaaggaa tgagtcgtga tgagttttta caagctctac cgtctttaga cgaagaattc
     6601 agcaagcgtg tagaagctgc caaggctgaa ggcaaagtgt tgcgttatgt gggctcgatc
     6661 acaggtgata aatgccgagt tgccattgaa gccgtggacg aaaaccaccc actttacaaa
     6721 gttaaagacg gcgaaaatgc ccttgctttt ctcacacgtt actacaaccc tattccatta
     6781 ttattgcgtg ggtatggtgc gggcaatagc gtaaccgctg ccggtatctt tgcagatatt
     6841 ctgcgtactt tacaaggagg agtgaactaa tggcgttacg aatttatgcc ccagcttcaa
     6901 gtgcaaatat tagtgtgggt tttgactcat taggcgctgc aatctccccg attgacggct
     6961 ctttgctcgg cgatgtagtt caaattgaag agagtgaaca acaatttgag ctagaaagtg
     7021 caggttattt tgtgcgtaaa ttgccaaaag aaccacaaaa gaacattgtt taccaagcct
     7081 atgtgctgtt cagtgaacgc ttaaaactgc gtggtggcaa cgtaaaaaat ttgcgtttaa
     7141 ccctcgaaaa aaatatgccg attggttcag ggcttggctc aagtgcctgc tcgattgttg
     7201 cagcattagt ggcactcaat aaattccacg atgaacagtt ctccaaaatg gaattactgg
     7261 aaatgatggg cgaattggaa ggcagaattt caggctcaat ccactacgac aacgttgccc
     7321 cttgctattt aggtggcctg caattaatga cacaatcgct cggcaatatt tgccaacctc
     7381 tgccattctt tgatgaatgg tattgggtgc ttgcctaccc gggcgtggaa gtatcaaccg
     7441 ccgaagcccg tgctattttg ccgaaaagct atactcgtca agatatgatt cagcaagccc
     7501 gctacttagg atctttcgtc catgcttgcc atacacatca agatatcttg gctgctacaa
     7561 tgatgaaaga cctaattgct gagccttacc gtgaagattt actgccgaac tttccggctg
     7621 ttcgtcaagg ctgtaaagac ttaggtgcgt tagcggttgg tatttcaggt tccggtccga
     7681 caatgtttgc gatagcccct gatctcgaac acgctcaacg tttagtgagc tatttagaga
     7741 aagaatatct gcaaaacaac gaaggcttta ttcatatctg taaagtcgat aatcaaggag
     7801 caagagagct gaaatagccc tctcgaacaa ccatatcact aaataactta aaggactgac
     7861 tccaatacca cttggagcag ccctttttaa ttaatttttt gaaaagtcta tcacttataa
     7921 acaatcgaag gctatgcctt acgccaaatt gtgccgtttg gggtgtcttc gaggacaata
     7981 tttatcgctt tgagcttatc tcgcacttca tcggcaaccg cccagttttt agccgctttg
     8041 gcttcgttgc gttgtttgat taaggcttcg atttctgccg tttcatcgtc attcgcatca
     8101 ccttttaaaa acgcatcggg gtcttgttcc agtaagccta gcacgccggc taactctttt
     8161 aaacgaacgg ctaaaccgtt ggcttttgcc atatcttcgg ttttgagttt gttcaactca
     8221 cgggcaattt caaataacac cgctaatgcc cccggggtgt tgaaatcatc gtccattgcg
     8281 gttttaaagg cttctacata gtgttctccg ccaatggctt cgcccgacac atcacaacca
     8341 cgcagagcgg tatataaacg ctcaagagcc gaacgggcta aatcaaggtt gtcaatgctg
     8401 taatctaata acgaacggta atgagcggtt aagaagaaat aacgtaacga ttcggcatcg
     8461 tataagccta acatatggcg aatgctgaag aaattgccca aagatttcga cattttttct
     8521 ttatcaatgg tgagcattcc agtgtgtaac caataattga cataatcgcc accgtgagca
     8581 cagcaagatt gtgcaatttc gttttcgtga tgcgggaaca ttaaatccga gccaccgccg
     8641 tgaatatcaa aatgttcgcc caactctttg ctattcattg ccgaacattc aatatgccag
     8701 cccggtctgc cattgcccca cgggctttcc cagcttggct caccggcttt cgacattttc
     8761 cacaagacaa aatccatcgg gtttttcttg atggatttaa tttcaaccct tgcaccggct
     8821 tgtagttgct ctaaattttg gcgggataat gcaccatatt tctggaacga ttccacatca
     8881 aacatcacat cgccatcggc tgcgacataa gcgtgtccgt tgtgaatcag tttttctacc
     8941 atcgcaataa tttcaggaat atgcttggtg gctcgtggct caacgtccgg gcgaagaatg
     9001 tttaacgcat caaaatcttt gtgcatttca gcaatcattc tatccactaa ttcatcgcaa
     9061 gtttcgttat tttctaacgc acgtttgatg attttgtcgt ccacatcggt aatgttacgc
     9121 acatagcgta aattgtagcc tgaataacgt aaataacggg caatcacatc gaaagagaca
     9181 aaagtgcgac cgtgcccaaa atgacaaaga tcgtaaaccg tgacgccaca gacgtacatt
     9241 cccacctgat ttgggttaat cggtttaaat tcttcttttt cacgttttaa ggtgttataa
     9301 attttgagca taaagtcctc ttgtgtaaca ttcagtaaca aatggggcta agtatagcga
     9361 ataagcggtc tgattttgag atttttttgc aatttggcgt aaacttttta ataatagcga
     9421 gcgaaattta ttttccattc catttttatt tataaaggaa caaagaatga ttacattaca
     9481 taccaatttc ggtgacatta aaatcgaatt aaactttgaa aaagccccga ttaccgctaa
     9541 aaactttgaa gattacgcta aagaagggtt ttataacggc acgattttcc accgtgtgat
     9601 tgacggcttt atgattcaag gtggcggaat gacggcaggt atggctgaaa aaccgacaaa
     9661 agatccgatt caaaacgaag cgaacaacgg tttaagcaac aagcgtggca caatcgcaat
     9721 ggctcgcact tctgatccgc attctgcttc gtcacagttt ttcattaatg tggcggataa
     9781 tactttctta gattatcgct caaaagagat gttcggcaga agtgtagtgc aagattgggg
     9841 ctatgcagta tttggcgaag tggttgaagg tatggacgtg gtcgataaaa tcaaaggcgt
     9901 aaaaaccggc aataaaggct tccaccaaga cgtgccggtt gaagatgtgg tgattgagtc
     9961 tgtaagcgtt gcctaattcc gcaaattagc ctacaagcgg ttgatttttc acaatttttt
    10021 gcatttctca gcgttgcaaa aaagtagcag aaatcgaccg cttgttttta tagaaattga
    10081 tacagccaaa taatcagcgg cattgtaaac acacaaagta aagtggttac gccataaatc
    10141 gcactggctt ttttggcatc ttgcccgtaa gccaccgcca tttgggtcgc cgttgcagca
    10201 gaagggctga tggtggctaa gaatgtaatc attaccaccg tttttccatt ctctacccag
    10261 ctcgcaaaac cgccgagttt aatcaccgct aacaaaatca gcggaataaa aatcaagcgg
    10321 agaaaagcca ccaagtaaat gcgtttatcg gtaataatct ctttaaatgg aattgctgca
    10381 attaacatcc ctgccacaag cattgctaaa ggccctaaca tatgccctaa agttgagagc
    10441 gtgtttgcaa gcgtagcggg cagtttgata tgtagcaaaa acaacatcat accaatcaca
    10501 atcgcaataa tgttgatatt aaagatcatc tttctaaacg gcacattgcc tttgccacaa
    10561 atcagcagcc gtaagtgaga ccaaaacaac acattttgca ccacaataaa agccgttgaa
    10621 taaatcaccc attcattgcc gaacagggag gcaacaatcg gaataattaa gttgccggag
    10681 ttggaataaa tagcggtggc ttgttctatg ttgtctaatt tcaacgctct tttgagcagc
    10741 caaccaagca gaattaacaa gccgtttaat aagacggaga ttaaaaatgc cagatacaag
    10801 ccgtttagta tttcggtggt gtaatctact tgaaaagcag taatcagcat tgccgggctg
    10861 ataatgtagt ccgataaccg aaatcggata actgtgctcc gatttgagta atttggtttt
    10921 aacgatgagg tagccgagca gcaccatcag ggttaattgt agaattttat cggctaataa
    10981 aagggctatt tccattatca attaagagcg agtctctctt ctcctgcata aatggtcgct
    11041 ttgcctttgc gggcaaaagc aagtaaggta aattgatttt cacgagccat attcactgcc
    11101 atttcagtgg tcgctgagat ggcacacaac atttcaatgc cgacggctaa gcatttctgc
    11161 accatttcaa agctggcacg actggttaca aacacaaatc caatcggaaa atcagcttta
    11221 gcataccaac cgattaattt atccaacgcc acgtgtctgc ccacatcttc acgaatggca
    11281 agcagttttc catccggggt aaaaaatgca gcggcgtggc ttgcaccggt ttctttcgat
    11341 aagaattgag cattttctag ctgtaataaa caatcctcta aaatcagtgg attgacgttc
    11401 tgcaagcggt tatttttctt aacaattttg caactttgat taacctgctc taattgctct
    11461 acaccgcaaa taccgcaacc tgttctgcct gccattgagc ggcgtttatc ttttaatgca
    11521 acaaattggc ggctcgatat ttcaagctga atttctactc cattttcttg ttcaattaca
    11581 tctaaaccat ataattccga tggggcattt aaaatccctt ccgccaatga gaatcctaaa
    11641 gcgaagtcct ccaaattagc cggtgttgcc atcattacag tatgagaaat accgttatat
    11701 accaaagcaa ccggcacttc tgaaattaaa ttttcttcat catctttttg tttaatacta
    11761 ttagttgata atgataattt tttggcattt atttttgtga tcttattcac aatatttatc
    11821 tctcacttga ctaaaaagta gtttattttg ttaaaaagtt gtatcattaa attgtttaaa
    11881 tttggtttac atcaaattaa tttaagattt tatctattta ccaaaaaaag agtatgttca
    11941 ttttctttta ttagggggaa aatgatacac ctcttagatc aatttattaa attaattttg
    12001 atcaaatttt attttatttc tcacattatg tgatttggag tgattatgca gatctcaaga
    12061 cgtaagttct tcaaggtctg tgcaggtggt atggcgggaa cttctgctgc aatgttgggt
    12121 tttgctccag caacagcatt agctgcaccg cgtgagtata agctcttaag agcaaaagaa
    12181 actcgacaaa cctgtactta ctgcgcggta ggctgtggca tgattatgta cagcttaggc
    12241 ggcgaagcaa tgaacagcaa aggaaagtta attcatattg agggtgattc tgaccatcca
    12301 gtaagccgtg gggcgttatg tccgaaaggt gcaggggcgt tggactatgt taatagtgac
    12361 cgccgtgtgc attatccgga agtgcgtgaa gccggttcaa gtgagtggaa acgcatttct
    12421 tggcacgagg caattgaacg tattgcccgc catatgaaaa atgaccgtga tgagaacttc
    12481 gtcacccaca atgaagcagg gctggaagta aaccgttgga tgacggcagg tttcttggca
    12541 ggctcggcgt gtagtaacga aaccggtatc gtcacccaga agtttatccg ctctctcggt
    12601 atgattttta ccgacaacca agcgagtatc tgacacggac caacggtagc aagtcttgct
    12661 ccatcatttg gtcgcggtgc gatgaccaac cactgggttg atattaaaaa ctccgattta
    12721 gtgatcgtta tgggcggtaa cgcagccgaa gctcaccctg tgggtttccg ttgggcagtt
    12781 gaagcgaaaa aacagaacgg tgcgaagtta atggtggtag atccacgctt taaccgtagt
    12841 gctgcggtgg cggatattta tataccattg cgtccgggta cggacattgc gttcttgtta
    12901 ggtgcggtaa actacctgct taaaaacgac aaaattcaac acgagtatgt gaagcattac
    12961 accaatgcca cattcttact caacgaaggg tttaaattcg aagacgggct tttcaccggc
    13021 tacgatgaaa ccactcgtac ctataaagac aaatccactt gggcttatca gttagatgaa
    13081 cagggtcagc cgaaacggga tatgacaatg caagatccac gttgtgtgat caatatgctc
    13141 aaagaccacg tttcccgcta tacgcctgaa atggtagaac gcattaccgg tacgccacaa
    13201 aaagatttcc tgattttctg tgaagagatt gcgaaaacct cagcgccgga taaagccgcc
    13261 accttcctct atgccttagg ttggacacaa cacaccgtgg gcgcacaaaa cattcgctca
    13321 atggcgatca ttcagttgct gtgcggtaac attggtgtct ccggcggtgg ggtaaacgca
    13381 ctgcgtggtc actctaacgt acaagggatt accgacctag gcttgttccc acatatgttg
    13441 ccgggctata tccgcctgcc aaccgaagcg gacgcaacct tagaaagcta cttaaatgcg
    13501 attgcaccga aaaccttagt ggcagggcaa gtaaactacg ggcaaaacaa cccgaaattc
    13561 gctgtgagta tgctcaaaac cttctacggc gataaggcaa ccaaagagaa cgagttcggc
    13621 tatcactatt taccgaaata cccgaaaggc ggtacagatc acttccgctt tatcgaacag
    13681 atgtatcaag gcaaagtgaa cggctatttc tgccaaggta tgaacccgat tgcctcttac
    13741 cctaactcgc agaagttaat taaagcgttg agcaatttga aatatttagt catttttgac
    13801 ccgcttatta ccgatacttc cgagttctgg aaaaatcacg gtgagttcaa caatgtgaac
    13861 ccgtctgaaa ttcaaaccga agtgttccgc ttgccgacca cttgctttgc ggaagaagat
    13921 ggttcaattg cgaactccgg tcgctggtta caatggcact ggaaagcagc agacgcacca
    13981 cacgaggcaa aaccggacgt ggatattctg tctgaaatcc gtgccgaact gatccacctt
    14041 tatcataaag aaggcggaaa agcaccgctt gaaccgttag aagcgatgac gtggaactat
    14101 atgaacccgt tagaaccgaa gccggaagag atcgccaaag agaacaacgg ttatgcattg
    14161 gaagatctga aagatgcaga cggcaacatc atcgtgaaaa aaggcgaact attatccagc
    14221 tttgcacaaa tgcgtgatga cggtacaacc tccggtggtt gttggatcta ctgtggtcaa
    14281 tggactccga aaggtaacca aatggcgaac cgtgataact ccgacccatc aggcttaggt
    14341 aacaccttag gctgggcatt ctcgtggccg gtaaaccgcc gtattctata caaccgtgca
    14401 tcggcagatt taagcggtaa accgtggaac ccgaaacgcc agctagttaa atggaacggt
    14461 aaaaactgga actatgtgga cgttgccgat tacggtacag caccaccaaa tagccctgtg
    14521 acaccattta ttatgcaacc ggaaggggta tccggcttgt tcgtgcgtga acgtatgcaa
    14581 gacggtccat tccctgagca ttatgaaccg tttgaaacgc caatcggcac gaacccgtta
    14641 cacccgaatg taatcagcag tccggtggca cgcattctgc cgagcgataa agcggattta
    14701 ggcaccagcg atgagttccc atatgtcggt acaacttacc gcttaaccga acacttccac
    14761 tactggacga aaaatgccct gattaacgtc attgctcaac cggagcaatt cgtggaaatc
    14821 ggcgagaaac ttgccgaaga gaaaggcatt aaaaatggcg acaaagtacg tgtttggtct
    14881 aaacgtggcg aaattaaagc cgttgccgtg gtgactaaac gtatccgttc attggtctct
    14941 gacggcaaac caatccacac tatcggtgtg ccgatccact ggggctttgc ggctacaacc
    15001 ggtgcgaaaa aaggctattt tgccaataac ttaaccgtgc gaacaggtga accaaactca
    15061 aatacacctg aatcgaaatg tatgttatta aacattgaga aaattgtggg ggcttaacaa
    15121 tgagtacagt tcaaactcaa aacattatta aaatctccgc aacctcggcg ctgacaccgg
    15181 cgccgcaggc tcgttcgcac caagtggaag tagcgaaact gatcgatgtc accacttgta
    15241 tcggctgtaa agcctgtcaa gtgggctgtt cagaatggaa tgacattcgc tccgatgtaa
    15301 atgcaaactg tgtcggcgtg tacgacaacc ctgcggatct aaatgcgaaa gcgtggacag
    15361 taatgcgctt taacgaagta gaagaaaacg accgtttaga atggttgatc cgtaaagacg
    15421 gctgtatgca ctgtgccgag ccgggttgct tgaaatcttg cccgtcaccg ggtgcgatta
    15481 tccaatacgc caacggtatt gttgatttcc aatccgacaa atgtatcggt tgtggctact
    15541 gtatcgcagg ttgtccgttc aacattcctc gtatgaatcc ggacgataac cgtgtttaca
    15601 aatgcaccct gtgtgtggat cgtgtcaatg ttggtcaaga accggcttgc gtgaaaacct
    15661 gcccgaccgg tgcaatccgc ttcggtagca aagaagagat gaagttctat gcagaacaac
    15721 gcattgctga tctgaaatct cgtggctatg aaaatgccgg cttgtacgac cctgaaggcg
    15781 ttggcggtac gcacgtaatg tatatattac atcatgccga caaaccggag ctttattcag
    15841 gcttaccgaa agatccgaag attgatacca cagtaacctt atggaaagac gttttaaaac
    15901 ctgtggcggc agtagcgttg ggtggtttag ctttagcaga agttgcacac tatattaccg
    15961 ttgggccaaa cgttgaggaa gatcttgaag aacaccacca tagcgaaaat gcagaaggag
    16021 gcaaacatga gtaagaaatt tgaaatcacg aacgatacca aaatcgttcg tcataagccc
    16081 ttagctcgtg ttagccactg gttcttagta atctcgtttt ttatgacgat gtttaccggt
    16141 gttgccttct tcttccctga ctttgcttgg cttaccgaga ttttaggtac tccccagatt
    16201 gcccgtgcag tccacccatt taccgggatt ataatgttta ttgcatttat tattatggcg
    16261 ttaatatatt ggcaccataa tatccctgag aaaaatgata ttcgttgggc aaaaggcatt
    16321 gttgaagtac taaaaggtaa tgagcataag gtttccgata atggaaaata caatttgggt
    16381 caaaaattac tcttttggac tcttattctt gcgatgttca ctcttttagc cacagggatt
    16441 attatgtgga gacaattttt ctctcactac ttctcgattc ctatgctaag aattgcaatt
    16501 ttacttcatt cagcaagtgc atttatgcta tttaccggaa ttttagtaca tatgtatatg
    16561 gcattctggg taaaaggctc aatcagaggt atggttgagg gttgggttac cgttcgctgg
    16621 gcaaaaaaac accacccaag atggtaccgt gaagaagtgc tacctgaatt agaaagacaa
    16681 gtagctgagc aaaataataa ataacctcaa gtgcggtatt attaccgcac ttttatttta
    16741 ttggtttaaa aagaatatta tgagtatcaa aatactacct caagatgata tcaaacaagc
    16801 agctagttca tttcagcaac ccgagctctt atttgctaat cccaaaaatc tgtatttacg
    16861 ccgcgctaag cggctacgtg aattagccaa gcaaaaccca ttcggtgaat atatggagtt
    16921 ttctgctaac ctcgtggaca ttcaattagc attacttgaa agccagccaa ttgcagatta
    16981 ttctcaaaaa ataaccgctt gtgtagagcg cacgcaagga gaaacaccgc taaatgcaca
    17041 gaattttcag cgtactgatg aatggcggac actactctta gccattattg agaaattcaa
    17101 gccttatgca aatgatactg ttcttgcaac aattgaagta ttggaaaagg cagctaagtc
    17161 agagctagac acacttgctg ataatttgct aaatgaacga tacgaacttg tgggcgcaga
    17221 taaagctgtc tttatttggg ctgctctctc actttactgg gtacaattag ctcaacaatt
    17281 accacgtaat tctcgtgcag aaataggcca taaacattta tgtccggtat gtaactcagc
    17341 acctgtaagc agtgtgattc atttcggtga agctcaaggg ttacgttatc tacactgttc
    17401 tttatgtgaa agtgaatggc atgtagtacg tacaaagtgt acaaactgtg atgaaaccgg
    17461 caaactggat tattggagct tagatacagt agaagcagca gttaaagctg aaagttgtgg
    17521 cgactgccat tcctacttaa aagtactcta tcaagataaa gacgtgaatg tggaacctgt
    17581 tgctgatgat ttagcaactc tattcttaga ttccgagatg gaacaaaaag gtctttcaag
    17641 aagtgggata aatccatttt tatttcaagt agagtaactc atttacaaca aaaacggagc
    17701 ataacgctcc gttttttatt acatttaact catacactat acttttttaa tcagctattt
    17761 ttttaacttc acagcggtta gcaataatct cccgatctga cataaatgcg tatcggcatt
    17821 gagatttaaa atattgaatc acagaatcat caagaggtcg ttgtgctcca acacgtaatt
    17881 cctccataga aaaattacta acctcttcat ctttctcttt atttttcaat accttacgtt
    17941 cagatattct aaacttaggc ttgtcttgct gtttttttac tccgtttaat ttttctttat
    18001 tactaggaat aggattactt gcataactaa tactattcat cgtaagaaaa agagaaataa
    18061 caaaaaagct aaaagcgttt ttcatagcaa acccataaat aaaatgtagc ataatcataa
    18121 aattacgtaa cattttatta catttagcgc atggttacaa attcttcaga tcctgtcggg
    18181 tgaatagcca ctgtgttgtc aaaatcagct ttcgttgcgc ccattttaat tgcaacagca
    18241 aaaccttgaa tcatttcatc aacaccaaaa ccaataccat gtaacccaac cactttttcc
    18301 tcttccccaa cacaaactaa tttcattcgg caaggttgac gatgttgtgt aaccgcagta
    18361 tacattgcag taaatgaaga tttatacact ttcacatttt ctgccccata ttgctcaatc
    18421 gcttttggct cacttaaacc aacagtacct attggtgggt ggctaaaaac gactgtcggt
    18481 actaagctat aatccaaatg ttcatttggt ttattattga acaaccgttc cgatagcctg
    18541 cgaccagctg caactgcaac aggagttaat tcaataccac cttcaataat atcaccaaca
    18601 gcatagatat ttggcacatt agtattttgg aatttatcca ctttgataaa accacgctcg
    18661 ttggtttcaa caccagcagc ctctaaatta atcttatctg tcgctggctc acgaccaata
    18721 gcccacacca agctttcaac agaggtttca cgaccatctt gcaattttaa tgtcaatgaa
    18781 ccatctgcat ttttaactac ttcttgagga gtcgcttgtg tatgtaattc gatcccatct
    18841 tgagctaata cttcaagcaa tgtatccact attaatgggt cttgattacg cattggagca
    18901 tgttgacgaa caaaaagatg ggtatttact cctaaactat taaatactcc agctaattca
    18961 actgcaatat accctgcccc cacaattgct acggacttcg gtaaagcatc tagtgcaaat
    19021 acaccatcag aagtgatacc gtattcagcc cctttcacat tgggtacagt tggacgacca
    19081 cctgttgcaa tcagaatatg gtctgctgtg acctcctcaa cggaaccatc agcataactc
    19141 acttctaatg ttttagcatc tttaaagcga gcaaaaccat ttaaaacatc aacattattt
    19201 tttgccagca cattaccata tgaggtatga atgcgcccaa tataggcctg gcgactctca
    19261 actaatttgg cataatcaaa attatttacc gtaatgtcaa aaccataatc aggggcgtaa
    19321 tggttaatcg cttctgcaac ttgtgcacca tagaacatca cttttttggg tacacatccc
    19381 acgttcacac aagtgccacc taaatgtttt gcttcaataa tggcacattt ttgcccatag
    19441 cttgctgctc gattaattga agcaatacca ccactaccac cgccgatagc aatataatca
    19501 taatgtttag ccataagcta ttcctcgatc tataaaaaca aaagcaactt attcgttaag
    19561 cgctttatca aattaaattt gcatagaaga ctaatgtttc atatgccctg aattcaacat
    19621 tttatcaata tacgccatcg ctaatgctga aacaataaag gctaagtgaa taactacttg
    19681 ccacatcatt tgcttttcat caagatttga agcattgata aatgtttgta ataaatgaat
    19741 agaagaaata ctgataattg ccattgaaag ttttactttc aaaatagtcg cattaacgtg
    19801 atccaaccat tctggctgat cagggtgatc atctactttt aatctagaga caaaagtttc
    19861 atatcctcca acaataacca taaccaaaag atttgcaatc atcaccacat ctattaaatt
    19921 taataccgct aacataacag tatttgaatc catttcattt aaattgataa ctagatgata
    19981 caatgattta ataaacttat acgcatatat cccctgtaca acaatcaacc ctaaataaat
    20041 aggtaattgt aaccaacgac ttgcgaaaat aattttacct aaaatatttg ccctagtatt
    20101 taattccgaa tgcatatatc tcctataaat agcaatacaa aaataaatta caacgctcaa
    20161 attttaacaa ttttttgcca aaaaattaag atatttaacc gcttgcacct tatattttaa
    20221 aaccttgtcc atcgttccaa gaaaccgtat attctagagg taaacggcaa cttggcaacg
    20281 ctgggtttgc tgctttaccg atagctagta gcataacagg caaataacgt tctgcatcta
    20341 tattcaataa ttttaataca gcagggcgct caaacacgcc tatcgcatga gtatcatagc
    20401 ccttatcttt ggcaattagc attaattgca ttgccgccaa actagtatca attaatacct
    20461 gatctctaat ttctgcttga gttgcttggt tatgaagatt tagtaaaaaa tcaaatttac
    20521 gttcacgaaa ctctttatgc aaacacccag tttctaccga ttgatcaaca attgcgccta
    20581 ataatttttc atattgcaaa tctgccaata tcaccacaat agcagacgct gtttcacaag
    20641 gtgggccatt aaaggcaaca ttttttgata atgctgcttt aagaatttca tcatcaataa
    20701 tgacaaaacg ccaaggttgc agatttgctt ttgagggagc aagctgggat aaagcgacca
    20761 tttcttctaa ttcagttcta ctaatcttaa ttgttgaatt aaaagctttg atagttttac
    20821 gctgttcaat cgtatgtttc aattccatct cttttctcct atctttcaaa caattgagat
    20881 agtctataat ttaaagatta ggaatgtaat aattgaaaag agttattctg catatttatt
    20941 gaaataggtc aaaattatca agtaatcata ttagagcagg agtaaagatt tagcttgccc
    21001 aagcatagga actaaggtgc tttccaaaat gactttcaat taaacgtttc gtaatttgat
    21061 gttgcggatt cagtaaaacc tctttcgtca agccatactc tactacttcg ccactttgca
    21121 tcaccattat tttatcggca atatgtttga tcagacctaa attttgcccc acataaatat
    21181 aagcaatccc ttgtcgctgc tggacactta acattaaatt aattaattgt gttttgactg
    21241 aaaaatccag tgaattaatc gtatcatccg caataataat ttccggttct aaaattaaag
    21301 cacgagcaaa cgcaatacgc tgcttttgcc cactggaagc ctctgaaata ggaacgagcg
    21361 cttgctcagg atacattcct accaatttta gcgtattaaa aatacgctca tttctttcct
    21421 cttcatttaa atctgtcgcc aagcggagcg gagcatctaa aatttgtcct atattataat
    21481 taggatcaaa ggcatcgtta gggtcctgaa acatcatacg aatatgctta gctcggtatt
    21541 tataatcgcc aaatgaaagc ggtgttcctc taaaaatcag ttctccagat gttggctcaa
    21601 ccataccagc aaccatttta gccaaggtag atttcccagc tccgttttca ccaataatcg
    21661 ctaaagtttc cttgcgattt agggtaaaag aaacttcttt taccgcataa aaatcttgtt
    21721 tatgaaatag gctactacgt tcaacaaaac gcttattcag atgattaact tcaagtaatg
    21781 ccataataaa ttggtgcttg gaaataaagt gatagtttac cgcaaaaaat aaataaaaaa
    21841 agaccgctta tacaagcggc ctgatttatg gaattttttg caaattataa aacgttcaca
    21901 cagtttaagt cttcgaaaga ttgctctaag cgtttagaca tagactcttc cattttacgt
    21961 aaccaaacac gtggatcgta gtattttttg tttggtgcat cagggcctgt cgggttacct
    22021 aattgacctt gtaaatatgc ttcgtttgct ttgtagaatt gaagaatacc gtcccacgca
    22081 gcccattggg tgtcagtgtc aatgttcatt ttgattgcac cgtagctgat tgcttcacga
    22141 atctcttcac gtgaagaacc agaaccgccg tggaaaacga agtctaatga ctttgccggt
    22201 aaaccacgct ctttagatac atactcttgt gaagcaccta agattgatgg ttttaatttt
    22261 acattacccg gcttgtaaac accgtgtacg ttaccaaatg ctgccgcaat cgtgaaacgt
    22321 gggctgattg ggcttaattg gtcgtaaaca tataaaactt cagaaggttg ggtgtaaagt
    22381 ttagattcat ctacatcaga attatctacg ccatcttctt caccaccggt tacgccgatt
    22441 tcgatttcaa gggtcatacc gattttgtcc atacgggcta aatattcacg gcagatttcc
    22501 atattttctt cgattggctc ttcagataaa tcgatcatat gtgaagagaa taacggacga
    22561 ccggtttctg cgaagtgttt ttcgcctgct tcaagtaagc catcgatcca aggtaataag
    22621 tttttcgcag cgtggtcggt gtgaagaatc acaggcacac catattcttc tgctaattga
    22681 tgaacgtgtt ttgcacccgc aatcgcccct aatacatctg cacgagcgcc tgaagccggc
    22741 ttaatgcctt tacccgcata gaactgcgca ccaccatttg agaattgcac gattaccggt
    22801 gattttacac gagcggctgt ttctaatacg gcattaactg aatctgaacc cacgcagttt
    22861 actgccggaa tagcgaagtt gtgttcttta gcataagcga aaactttttg tacgtcgtcg
    22921 cctgttacta cacccggttt tacgatgttt aataatgaca ttttttgctt cctttgtatg
    22981 aaatgtgcaa aagcacattg ttaaaatata cggccaaatc ttgcaaaatt ttcacaaaat
    23041 ttgaccgctt gtaactgtta gccgttagct cgtttttcta agatttccac tgccggtaat
    23101 actttacctt ccacgaattc taagaacgca ccgccaccgg ttgaaatgta agaaattttg
    23161 tctttgatac cgaataaatc aatcgcagct aaggtatcgc caccaccggc aattgagaat
    23221 gcaccgtttg ccgttgcttc tgcaatcgca tttgaaatca cttctgttcc tttacggaag
    23281 ttagggaact caaacacgcc gaccggacca ttccaaagga tggtttttga attacggata
    23341 atttcagcta attgctctgc cgatttttca ccaatatcga aaattgattc atcagctcgt
    23401 acttcactca ccgctttttc cgttgccggt gcagtttcag agaattcagt acctacacgc
    23461 acatcaaccg gaacaggaat attggttact tgtgctaaac gttttgcttc agggattaaa
    23521 tcttcttcgt ataatgattt acccacagcg tgaccttctg cggcaataaa ggtattggca
    23581 ataccaccgc ccacgattaa ttggtcggca atttttgaaa gcgaatcaag cacagttaat
    23641 ttagtagata ctttagaacc acccacaatc gctaacattg gacgttgtgg ctcttttaat
    23701 gctttgccta atgcatctaa ttcagcggct aataacggac ctgcacacgc aacaggggca
    23761 tattctgcca caccgtaagt tgaaccttcc gcacggtgag ctgtaccgaa tgcgtccatt
    23821 acaaacacat cacaaagtga tgcatatttt tttgctaact cagggtcgtt tttcttctca
    23881 cctttgttaa tacgcacgtt ttcaagcacc acgatttcat tctcgtttac ttcaacaccg
    23941 tctaagtaat cacgcactaa acgaacagga acacctaaat ctgatgcatt taagtaatca
    24001 actacaggtt gtaaagagtt ttcctcttca aatacgcctt ctgtcggacg acctaaatgc
    24061 gaagttacca ttactttagc acctttttgt aacgctaatt ttaaggttgg aatggtagca
    24121 acaatacggg catctgatgt tactttgcca tcttttaccg gtacgttaag gtcagcacga
    24181 ataaataaac gtttacccgc taaatcaagg tcagtcattt taataacaga catagtttgt
    24241 tcctctttag attaaaattt aaaagtgata acggtcggat tatatcagtt tctaatcatt
    24301 ttgggctaac ctagatcaaa taaatgatta attttttatc attttagaat gctattatcc
    24361 tgactgttct tatttccccc ccaatatgct tggttaatta agcaagaatt gcaaattccc
    24421 cacaaaattt aaccgcttgt cactaaaaat aaagctgttt gaaaatccat tcaaacagct
    24481 tatagaattt agagtgaatt aaagaaaatc accaagataa tcaccggaat cacaaagcga
    24541 acatagttaa accaaatagt ggtaaacatt ttacccgcac ctaattcctc cttcgcctct
    24601 tcttttagaa caaagccaac gaaaatcgcg ctacctaagg cggttaaaat aaacagaata
    24661 ttgccactaa tataatcgaa ggcatcaaaa atgctcttgc ccataacctg cacatcttgc
    24721 aatatattgt cacttaatgc tgatggaata ttacccaaca gaaaaatagt agccaaagta
    24781 ataaaaatag ctttttgacg gctaatcttt gccttctctt gcaatgcagt gataataact
    24841 tcataaatag ttaacgaggt tgttaatgcc gccaccacta atagactaaa aaagataacc
    24901 gcaaaaactg ttccccccca catatttgag aacacaatcg gcaaactttg gaatactaaa
    24961 gtcggacctg aatttggagc aacgccaaag ctaaataatg acggaaaaat cataaaaccg
    25021 gcaagtaccg caattaaggt gttcatcacc ccagtaattg ttgctgtttt tactaaattc
    25081 tcgctcttat caaggtagct tgaaagggtt atcaacacac caaaaccaag gcttaatgca
    25141 aaaaatactt gccccagcac aaacacaaac aattttggtg tgatttttga aaaatcgggc
    25201 atcagataaa aggcaatgcc ttcagcagcc cccggtaagg taatatttcg aaccaccatc
    25261 acaatcagga aaataaacag aatcggcatc aaatatttca ctgaacgctc aataccgtcc
    25321 acaatgcctt ttaccaaaat aaaataattc accaatacaa aaagtgccgt ataagcaata
    25381 attgtcattg ggctattaaa aatactttgt tggaagaatt gttgggtcgt gccaacggta
    25441 acaggttgag acagatctag tgtccccgtg agtaagccac cgatataatt taatacccaa
    25501 ccaccaagta ccatatagta ggcaagaata ccaaatgctc caagcaagcc catatagcca
    25561 agaattttcc aacctgaggc aattttttta ccattagcaa aaccggcaaa agcatcaata
    25621 gcattcactc tcattcggcg accaattaca ttctccacta atatcatagg gataccaatt
    25681 acgatcatcg caatacaaaa taaaaacaca taagcaccgc ctccgtgttc ccccacaaga
    25741 taagggaaac gccaagttgc accaaaaccg actgttgcac cagcaacggt cataatatac
    25801 gccaatcggt tagaccaaga ttggcgttct tgttgttgat ttgccattta atactccatc
    25861 ggctaacaat accgcttcaa ccaacaagcg gtcagatttt tagaaaatat tacaaattaa
    25921 atgaagagtg cgaataactc gccaacttta acccttgagc ccaccttctg ccctattgaa
    25981 cgctgtacct gaatttgggt aatatcggca gattgataaa tcctacgggt aaaatcagta
    26041 tcgtgatttg aaattaatac gggaacgcct aatcctttca tttctaaagc cagtgttgcc
    26101 aaacgccctt gttgctctag gctaaaacca ccgccagcat attgcgtaaa attcgtttct
    26161 tgtgctaaag gagcgtaagg tgggtcgcag taaatcacat aatccgttaa ttgatttttt
    26221 gccaactcaa agacctgttc aaaatccgca cacacaaaag ttgccttttg tgctttttca
    26281 gcaaaaaaac gtagttcttt ttctggaaaa taatgggttt tataacgccc aaagggaaca
    26341 ttgtaccctt ttttctggtt ataacggcaa agtccgttat agccaaatcg atttaaatac
    26401 aaaaaaatca ccgagcgacg aaaaatatct tttgagcgat taaactccgc acggcatgct
    26461 ttataaaagg cttctgtatt cgcttgtggg tgaagaaaca gttgctttgt ttgcgcaata
    26521 tattcctcaa catctgcttt caccacgttg aaaagattaa tcaagtcagg attaatatca
    26581 gcaagaatat aacgttcaaa atctgtattc agaaagaccg aacctgcacc aacaaaaggc
    26641 tcgactaaac aagccttttt tggcaaatgg gtttgaatat ctggaattaa gcgatattta
    26701 ccgccagccc attttaaaaa ggctcgatgc ttagggtgat tcatttcatt ttaaacatta
    26761 acgttgggta aaacgctcaa tagaggctaa tacttgagat tctgtggctt gcgatgaaac
    26821 gtaagcttca ccaagtgatt tcaataaaat taggcgtaat tgacctgcca gtactttttt
    26881 atctcgccac atataaggta gatattctgc cggttccatt ccatcaggtg aaattgttgg
    26941 caaattggct cgggccaata atttttccaa gcgagcaacc tcttgaggac ttaaatcccc
    27001 cagaatacga gataattccg cagcctctag cataccaacc gaaacagcct ctccgtgtaa
    27061 ccatacacca tagcccatac gtgcttcaat ggcgtgacca aaggtatgtc ctaagtttag
    27121 taatgcacga tctccttttt ccgtttcatc tcgagcaacc acgtcagctt ttagctggca
    27181 acaacgctga atacaatatt ccagctcatc ttgcttgaga ctgaccaaat catcaatatg
    27241 cttttcaagc cattcaaaaa aagtaacatc aaagattgca ccgtatttga tgacttccgc
    27301 caaaccagca ctgacttctc ttttggataa ggtatggagc gtattggtat caacaatcac
    27361 agaaacaggc tgataaaaag ccccaatcat atttttaccg agtgggtggt ttactgccgt
    27421 tttcccgcct actgaagaat caacttgagc cagtaaagtg gtcggaattt gtataaaacg
    27481 aataccacgt tggtaagagg cagctgcata gcctgcaaca tcaccgatta caccaccgcc
    27541 taaagcaatt aaagtggtat cgcgattatg atttttttct aataatgcgg taaaaatcaa
    27601 atttaatgat tctagcgttt tatattcttc accgtcggga atcaataccg aatcaacgtg
    27661 gcaacccagc tcctttaacg catttgttac cacactaaga taatgtgtcg ccaccgttgg
    27721 atttgagaca atcatcacct tatctccaga ttttaatggg ctataactag caggctgatt
    27781 taataaccct gcaccaatta taatcgggta acgacgctct tttaattcca cattgacttg
    27841 taacatatct tagctccttt cttacctggc tactgtaaat tatcaatcat atcaataatt
    27901 tgatttgcca ctactttagc gctctgctcg tccgtatgaa tagtaatatc tgcaatttct
    27961 tcatacaatg ggttacggat tttcgctaac tcttccaacg ttttacgggg gtcttctgtt
    28021 tgcaataaag ggcgtttttt atctcgctga gtacgttcaa actgtttatc cactgtcgtt
    28081 tctaaataaa taacgatacc acgagccgat aacacattac ggttatcttt agataaaatc
    28141 gaaccaccgc cagttgaaag cacgatacca taattttgcg tcagttcatt tagaatacgt
    28201 tcttcacgct tacggaaacc ctcttcacct tcaacatcga agatccaatc aatatccgca
    28261 cctgctcgtt cttcgatcac actgtcggaa tccataaatt ccatacctaa tgtttgagca
    28321 agctgacgac caatcgtact tttacctgct cccatcggcc caatcaaaaa aatattacgt
    28381 ttttcagcca ttatttcttc ttcattttaa aatttactaa acatttgatc tgtgaaatta
    28441 aaagaaaaaa tgaccgcttg ttctacaagc ggtctgattt ctttaaaaaa ttgcaataaa
    28501 acccatttat tgactaagga atatacaaat ataactcaca aatcgcccaa aataaaattg
    28561 gcacttatta tcgcaagaaa tccccctatt tttcaacttt taagagcatt atccttgtaa
    28621 taaattatta gaataattca ccgcttttta gcacccctgg tggaggtaat cctaaacgta
    28681 acggcgcaga ttctgttttt tgaggcgctt tttcagtata agctctctct acaatataac
    28741 gttttgtagg ctcagtaccc ttaataaaat actctttacg tccatttcct aagaaaccac
    28801 tattagggtc aatattaacc tcaataatat tttttggaag ggtacttttt tcgacaggct
    28861 tatctgacaa tgcaactttc atataatttg tccaagctgg taaagcagta ctagaacctg
    28921 aagcacctct acctaatact cttttattat catcaaagcc aacgtacaca acagtagaaa
    28981 tattagcacc aaatcccgca taccaagttg ccttggcatt attggttgta ccagtcttcc
    29041 cgcccacatc cgctcgtttt atcgtattga gcattttata gcttgttcct cgccagccat
    29101 attcaggctc accagtaatt gctgtagcta aagcactacg cattaaaaaa gcgagttcat
    29161 cactaataac atgaggagcg taacgtaacc ctttgacatg atgagaagat tctgccatta
    29221 agctcggcgc atttttctcg ctattgttcg cttgtaattc aggttctgaa tcgccaacat
    29281 cttccacaac atcgtcgtca tcattattcg aggaattcgc tatagtttgt acaccgactt
    29341 gagcttcatc atgaattttg actgattcaa aatatttagg ctcagggtaa acaactgggc
    29401 tatcgcaact attacaagct actacaggat ttgcttgata taactcgtag ccttgggcat
    29461 caataatacg gtcaatgata tatggttcaa tcaaataacc tccattatta aatactgcat
    29521 aagctcgagc catttctaat ggcgtaaaag atgcagcacc caatgcaaga gattctgttg
    29581 caacatactg atttcggtta aagccaaaac gttgtaagta gtctgcaaca taatcaatcc
    29641 ctgccatttg taatatacga atagccacca tatttttcga tttacctaat gcaacacgag
    29701 ttctcaacgg gccgccataa accccatcgg catttttagg tcgccactct ttttgcccct
    29761 tctttttaat gacaatagga gaatcgctaa ttgttgttga aagacttagc cctttattca
    29821 ttgccgctgt gtaaataaac ggtttaatcg cagagcctac ctgaactaat gactgagtcg
    29881 cacgattgaa acggctttgt tcaaagctaa agcctccaac gatagattca atggcaccgt
    29941 tttcactatt aattgacact agggctgaat ttacctccgg aatttgtcct aaagcccact
    30001 ccctattttt attttgacgt acccaaattt gttcacctgc ttttaaccct ttaggtgcaa
    30061 aagaagcatt tgtacgtttt aaaacagaga tatcaccatt tgccaataaa attgtatatt
    30121 ttgatttatc tgcttgcatt acaacagctg gtgtaaacgg ctctgatttt ggcagcttac
    30181 ttaaatgatc gataattttc tcatcgtccc aagcagaact acctgtccat aatttttcag
    30241 cgcctcgcca accatgacgg cggtcataat caatcaaatt ttcacgtaac gcatcttgtg
    30301 ctgctttttg gtctgctgaa agtaccgtgg cgtacacttt taagcctttg ttataagcaa
    30361 tattttctcc atagcgtttc acaatttctt gacgaaccat ttctgtcaca taatccgctc
    30421 gaaattctaa tgtcgcacca tagtagttag ctactactgg ctctgcttta gctttctcat
    30481 attcctcttt tgtaatatct ccggtttcta gcattctccc aagcactaca ttacgacggt
    30541 cttccgcccg ttttactgaa taaagtggat tcatcgttga aggcgctttt ggaagccctg
    30601 caataattgc tatttcagaa agggttaaat ctttcaaagc tttattaaaa taagttttag
    30661 ccgcagcggc aacaccgtga gaacgatacc ctaggtaaat tttgtttaaa tacagctcca
    30721 gaatctcatt tttactgaga acctgttcaa tttctaaagc taaaattgcc tctttggcct
    30781 tacgctctaa actacgttca ggtgttaaga aaaagttttt cgccagctgc tgggtgattg
    30841 tacttgcgcc ttgagtatct ccctggctag tgcgccatat tgcacgcata atacctttag
    30901 gatcgatccc tttatgctcg taaaaacgtg catcttcggt agcaagaatc gcttttacta
    30961 acatcggtgg aatatcatca agcttgaccg gaatacggcg ttcttctcct acttcaccaa
    31021 ttaatttacc atctaaggta aaaatctgca taggctgctg taactctaca tttttcagcg
    31081 ttgccacatc aggcaaatca gatttgaaat gcatgtacag taaaccacca attaccccac
    31141 ctaaaaccaa gagtgaaagt aaagcactaa atattatttt tgcgatccgc atcgaaaaaa
    31201 tctcttttgg ttaatgacga aacataaatt taaagccaat attaggtcac taagtataag
    31261 ctaaaaacgc ttacttcgca taactaattg caaaacttta cttactatac ttactatgaa
    31321 attacttaaa gcacattcaa aaacaccttc aaaccgtatc atcttaggct taagtgaaga
    31381 tgaacaacgt tactgcttag tcaaaaataa agcagaaaaa ttcaccgttt tttggcaagc
    31441 caagcctgcg agcatcgaag agcttatcgg taatctcacc ccacaagaac aagcaatgat
    31501 gacgctggtt cgcccgatcc cttaccaata catttggcga aaaaccgttt ttatggctaa
    31561 caccctcaat gaagctcaat tacaccgtaa ggtaattcac attttaaaaa atgagcagcc
    31621 acttgcattg gatctactca atattgacta tcagcgattt ccaagcagca acgataatct
    31681 ggataaagtg gttatctacg ccgtgcgaaa aagttatacc gagaatttga gccaatttaa
    31741 cagcatctta gattgtgagc tgcattgcta tgcacgggct ataattcatt taacacaatc
    31801 ccaaaataat cagcctgcgc cctgtttttc atttaaacag aagtacttcc aatttaccga
    31861 gacggaatta ctgatattgc aaacagaacc tgaggattgt attcatctat ctgatctcaa
    31921 aattgaccat cttgaaatcg agtgtgagca agagaaacag ctatattgcc tcgccttagg
    31981 agcaagttta tggaatggca aggtattgat ttaagtgcta atcgaaaaaa taactggttt
    32041 gccggcagtg ataaaggcat caaaaacgtg atttatacac ttttattatc tgccctcatt
    32101 agcttggctc tgatttttct ctatttgcaa aaaaatcaag aaattcaacc gcttgcacag
    32161 caattagagc tggtaaaaac cgatatatta aatattgaaa ataaaattat cagcttgaat
    32221 agtaatcata caaacacctt gccctttgcc gagcctaaaa aaatagcaca actactggca
    32281 ataatagata acttaccgct caaaaatggg ggaattgaaa cgatacaaat ttatcttgaa
    32341 accgatctct atttaaaaat aatcggtaat ttagataatc aatctgattt tcaaagtcta
    32401 gaatcttatt tatatcagca agatttattt gaaaccaaaa ccgagcaaat taatgttaat
    32461 cataaaaatg aaatccaatt tattttcaca ctgaaacata aaggtaatta atatgttttc
    32521 tatacattct aa
//
LOCUS       DANKBV010000010        32520 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.010, whole genome shotgun sequence.
ACCESSION   DANKBV010000010 DANKBV010000000
VERSION     DANKBV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 32520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32520
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.010"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1942)
                     /locus_tag="PWY43_000379"
     CDS             complement(<1..1942)
                     /locus_tag="PWY43_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL2135293.1"
                     /translation="MKNRNYFVAIATILALSPEVNATELSDQLASRTRYYPSATDSYY
                     SYYKYAPIYSGYYTSNSRNGYSSWYYPGSNAGVTTRTVSTTVNGITVTKVYTTHNGRT
                     TVRTTYAPAVNKPIVNKPTPIANKPVVDTPTQPVIDANPIMDPPVQPIVDNKPITDTQ
                     PNPITDNKPVIESPIDDTRPVIIRPTTPVNLHPEPVAVGVPNVGIRWNDRSRGYNIDD
                     PNNKQNVKLTGEGVKVGILDSGFKNYLMQDDILKKFGNRAVIIETGRTAPASATHGIE
                     VAEMVGGGNSRNGVASKSTLLLADITKTTNAGTGLSATAPIYYDLWNRGARIFNQSYG
                     IPKPLTYFNNNRNSSYYYLHQFDGNVLQFYKDKVKEGGLFVWAAGNNRGDLDPSLQAG
                     LPHYEADLEKGWISVVAVATRTNQGLGNFEWSNLLPYSQAGVAKNWTVTAMGDYIFNL
                     GGRNIIASGSSFAAPAVTGTAALVKQKYPWMDGNLIKQSILSTATDIGAPGVDDVYGW
                     GLLNIEKATNGPARFDKRLALADNVTVNIPNGSYEFSNNINGDAGLVKNGQGELILSG
                     ASTFEGDTTLNEGAVTINGKKYVSKVNVSPSGTLVVNNTTLENGVNNEGTVVNQGYST
                     IKRAYVASAQSTLVSDLGSNLNV"
     gene            complement(2190..2909)
                     /locus_tag="PWY43_000380"
     CDS             complement(2190..2909)
                     /locus_tag="PWY43_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2135294.1"
                     /translation="MTQLTELTPALQALRERVLKANLELPKYGLVTFTWGNVSEIDRE
                     TGLVAIKPSGVEYDAMTVEDIVLVDLQGNRVYGDKKPSSDTETHLELYRQFTDIGGVV
                     HTHSRNAVGWAQAGRDLLALGTTQGDYFYGAIPCTRKMTAEEIAGSYELETGKVIVET
                     FRTRGIEPKAVPGVLVHSHGPFTWGKDGHDAVHNSVVLEEVAYMNFISHQIRPQIGPM
                     QQELLDKHYLRKHGANAYYGQ"
     gene            complement(2906..3766)
                     /locus_tag="PWY43_000381"
     CDS             complement(2906..3766)
                     /locus_tag="PWY43_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL2135295.1"
                     /translation="MRKHKLGIYEKALPKNISWQDRLSIAKVCGFDFVEMSIDETDER
                     LARLDWSKKERAKLVKTIINTGVTIPSMCLSGHRRFPFGSCDEATRQKAYEIMEKAIQ
                     LAVDLGIRTIQLAGYDVYYEEQDEGTIQRFQEGLEWAVELAASNQVTIAVEIMDTQFM
                     SSISRWKKWDEIIKSPWFTVYPDLGNLSAWNDNVAEELKLGIDKISAIHLKDTYKVTE
                     TCKGQFRDVPFGDGCVDFAACFKTLAELNYRGAFLIEMWTEKAEEPITEIINARRWIE
                     QKMQEGGFTL"
     gene            complement(3824..4570)
                     /gene="ulaR"
                     /locus_tag="PWY43_000382"
     CDS             complement(3824..4570)
                     /gene="ulaR"
                     /locus_tag="PWY43_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDL2135296.1"
                     /translation="MNESYRHKELLALLEERKALSTADIMQLLNISPATARRDISKLD
                     GQGKLKKVRNGAEAILASTVFTAHKITNNAQEKRRIAEAAGRLCKDGESLILTCGSTM
                     EMLGEVLCGRKLQIITNYLPLANQLIAGCHDDVVIMGGQYNKHKKVMLSINTQNEMTY
                     AATTMFTSGKGLTSEGLFKTDMIIANAEQQMAKHAERYVVLLDSTKLGKKVGMLFKEF
                     DKIDLLITGKEADPTIIQELRNRGLDIILA"
     gene            complement(4654..5745)
                     /gene="ulaG"
                     /locus_tag="PWY43_000383"
     CDS             complement(4654..5745)
                     /gene="ulaG"
                     /locus_tag="PWY43_000383"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL2135297.1"
                     /translation="MSKIQEITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     CVGVWIKTPAGANICMDLWCGRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVL
                     DPFAINEVDFILASHYHSDHIDVNVVAAICNNPKLDHVKFVGPWHCAELWKKWGVPEE
                     RIIVVKPGDVVKLKDVEIHALDSFDRTCLVTLPVEGAEERGGELAGLCPSDEEMGRKA
                     VNYVFKTPGGTIYHGADSHYSIQFAKHGKQFDIDVALNNYGENPVGIADKMTSVDLLR
                     MAECLRCKVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYQFHPFIWEVGGKYVYPR
                     DKDLIEYHHPRGFDDCFEQEPNIQFKSML"
     gene            6128..7923
                     /locus_tag="PWY43_000384"
                     /pseudo
     CDS             6128..7923
                     /locus_tag="PWY43_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate-specific subunit IIBC"
     gene            8003..9064
                     /locus_tag="PWY43_000385"
     CDS             8003..9064
                     /locus_tag="PWY43_000385"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2135298.1"
                     /translation="MKKILSTLFMLSAVSVAVAKEVNIKLLGTSDVHGRVVPWSYGAD
                     VEDKSGSFAQIATYVKEVRANHKNVLLFDIGDAIQDNQVEEFAKTLDKAKNNPVPKVL
                     NAMNYDIFILGNHEFNFGMPNLDAILKDINAKILTANFYHKDGKRYVEPTTIIERDGV
                     KLGIIGLTTPMSAIFEKDTGHLDAMKFTSPIEEAKAQIADLKAKGVDAIIVLAHMGIE
                     NENNIPDTGVADLVNAVDGIDVIIAGHMHKDVPSETIKNTLITEPHRYGTVVSEVDLA
                     FDVNDKGEVKLLSKTAKTVPVKKLASDPEIEKIYQPYHNELRALNNVKIGETAQDLIP
                     QGKITVYRLLSRKTPECPH"
     gene            9052..9516
                     /locus_tag="PWY43_000386"
     CDS             9052..9516
                     /locus_tag="PWY43_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDL2135299.1"
                     /translation="MSSLIHDVQQHYSGADVVSFAFDYETVRLDKGDIKKKDIIYNYR
                     YAGGDVSMYELTGKQLKQYMEWSADYFDTIQAGDKDYRYNAVRGKSKYVTFDLFGGVS
                     YNIDLRNPSGSKIVDLKLANGSLITDDGKYKVGMNSYRFGQLTKKGGIWEGQ"
     gene            9547..9663
                     /locus_tag="PWY43_000387"
     CDS             9547..9663
                     /locus_tag="PWY43_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser/Thr protein phosphatase"
                     /protein_id="HDL2135300.1"
                     /translation="MGAEKGTIQNMLIDYITNVKGGKIEGKSHNRWAIIGLN"
     gene            9679..10146
                     /locus_tag="PWY43_000388"
     CDS             9679..10146
                     /locus_tag="PWY43_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2135301.1"
                     /translation="MNLKQSLIENNSILLNQSAANWEDAIKIGTDLLVKAGTIEPRYY
                     DNIVSKIKEMGPYIILAPGLAMPHARPEEGVIKTSFALVTFDTPIYFEGEDEPVHVML
                     TLAGSDSDQHMQGLVEITQVLDDPESDTGVDLDKIRRCKTAEEVYAVIDQALA"
     gene            10201..10881
                     /locus_tag="PWY43_000389"
     CDS             10201..10881
                     /locus_tag="PWY43_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL2135302.1"
                     /translation="MSKPLLQIALDSLSLEKAVADAKQAENQVEIIECGTILAFAEGM
                     KAVSTLRALHPNHIIVCDLKTTDGGAILAKMAFEAGADWLTVSAAAHPATKAACKKVA
                     DEFNAAHPELKVKKEIQIEIYGNWTVEKDAKEWVELGVTQAIYHRSRDAELAGKGWTT
                     EDVELMKQLSDLGIELSITGGIVPEDIHLFKEIKNAKAFIAGRALVGEKGKQTAEAIR
                     VEIDKYWN"
     gene            complement(10949..12091)
                     /locus_tag="PWY43_000390"
     CDS             complement(10949..12091)
                     /locus_tag="PWY43_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2135303.1"
                     /translation="MKIVIAPDSFKESLTALEVAQAIQQGFARVFPEAEYQLVPMADG
                     GEGSVQSLVDATHGSLQKISVIAPLANEVEGFFGLSGDQKTAFIEMAAASGLHLVPME
                     QRNPLVTTSYGTGQLIKAALDLGVEKIILGIGGSATNDGGVGMLQALGAKFLNAEQRE
                     IGFGGEALSQIQQIDLSGLDSRLKQVEFEVACDVDNPLCGERGASAIFGPQKGATAEM
                     VKTLDHALLHFAETVKSQLGIEIAEKAGAGAAGGMGGGLLLLPNVTLRSGVQIILEAT
                     DLAEKLADADLVVTGEGRMDAQSIAGKTPIGVAKVAKQFGKPVIAIVGSLKEDYPVVY
                     QHGIDAVFPIIRQVSNLEAVLKSGKENLISTAENVARLWKISASQE"
     gene            complement(12101..13480)
                     /locus_tag="PWY43_000391"
     CDS             complement(12101..13480)
                     /locus_tag="PWY43_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL2135304.1"
                     /translation="MSGIALILIFLVAIIIMIYMIAKWKVHPFLALMSISIALALVAG
                     LPLAKIPTIVGEGFSNTFKSIGIVIIFGAIIGTILEKTGAALKLADMVVKVVGQKRPE
                     LAMLIMGWVVGIPVFCDSGFVVLNPIREAIRKKILANPVGMAVALSAGLYASHVFIPP
                     TPGPIAAAGAVGLSHNLLLVIGMGFVVSIPVLIASYFFAKYVGKQVSVMEEAQADQVI
                     SQSYEDLIKQYGKLPSGFLSLAPIFMPIILMAIGSIAKIAGVSGDFGVFLQFVGNPII
                     ALAIGVIFAIFLLVSSGKMAEFDSLTNDTLKLVGPILFITAAGGVLGNVITQAGFVDY
                     IKQNAHIISTAGIFFPFLISAILKTAQGSSTVAIITTASIMGMFNAPDSLMNALGLTS
                     EMAAALTVMAISAGSMCVSHANDSYYWVVTNFTKLTPQQGYRTQTTLTFIMGVVGMLT
                     VYLLSLILL"
     gene            complement(13602..14696)
                     /locus_tag="PWY43_000392"
     CDS             complement(13602..14696)
                     /locus_tag="PWY43_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2135305.1"
                     /translation="MKLNQAIADRIVKRTMQIIPNSVNVMDSEGVIIASGDVGRIGQR
                     HTGAVIALRNDQAVEIDENLAKQWCDEVKAGINLPLHYLGSTIGVVGISGQPQQVRQY
                     AQLVKMAAELIMQQAFELEQERWQRRYREEFVRALLKGSLSTAEIQQQAVFFGQDFAK
                     PLSVMVIKIHGSTAEKLQRLIDFFEHYFPSLLTAVIGLDKIALLNHLSNEKSLIRNAL
                     NKLNDPDISFKTVVGLAVENLSQTHISYQTACHTLNYAEQIQSRKQIIHFEDFKLPAL
                     LHSFSHSWEAKTLLSPFQALLVSDKKQVLLKSLRYYFFANCDLDHAAKKLFIHPNTLR
                     YRLEKIEQITGLSFNNIEQKFVLYLGAVLS"
     gene            complement(14800..15252)
                     /locus_tag="PWY43_000393"
     CDS             complement(14800..15252)
                     /locus_tag="PWY43_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HDL2135306.1"
                     /translation="MNLKAVAVSALAMSSVAMADIADVKPVVFESKFDFQTTVTTLTN
                     TLKEKGMTIFAEIDHQAAAKQAGLEMQPATVLVYGTPKAGTPLMVKDPMLALQLPLKV
                     LITEPNKGKVEVMLNRADQVVAHANTDYADVENNLAKAEKLIQATVAK"
     gene            complement(15273..16025)
                     /locus_tag="PWY43_000394"
     CDS             complement(15273..16025)
                     /locus_tag="PWY43_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL2135307.1"
                     /translation="MHFEKRSHWSGELGREMSFNVYGHAGKPVIVFPSSGGNENEYGN
                     FGMIEACRSFIERGLIKFYTPDSYDAESWLSKHKSGHDMALAHNAYDRYIIHELVPLI
                     RHESHWNGTMIATGCSMGAFHTVNFALRHPDLFDIAIALSGVYDARFFSGEFYGDKAV
                     YFNSPIDYVWGQNDDWFLDRYRQNHYVIAVGQGAWEEQHIEDTRRLQEAFNAKGIDAW
                     FDYWGFDIDHDWPTWRKQMPYFLGKLEEQGII"
     gene            complement(16036..17220)
                     /locus_tag="PWY43_000395"
     CDS             complement(16036..17220)
                     /locus_tag="PWY43_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL2135308.1"
                     /translation="MSRPLNFVMISPHFPTNFETFAIRLREAGFNTLGIADAPYESLS
                     ENLRHSLTEYYRVDNMEDYEQVYRAVAYFAHKYGRIDRIESHNEYWLELDAKLRTDFN
                     VFGYKSDDMLSIKTKAQMKEVFRKTGLKVAQGRVFKDDEDACKLAKQLKFPVIIKPNS
                     GVGASDTYKIKTAEELEAFFGYKTPTVEYIMEEFIDGDIITFDGLTDKEGNIVFYSSL
                     EYSEAVLDTVSKDGDMFYYVPCEISPKLVEMGKQCVKAFNVKERFFHFEFFCVKGSKK
                     EELLPLEINCRPPGGLTIDMWNYANDFDVFREFANIVKENKFYADIQHPWNVVYISRK
                     ANQNYVNSIEAVCEKFAGNIISVQSVPGVFAKVMGEQGILARTETMEQMREIAQFAQA
                     KQ"
     gene            17513..17944
                     /locus_tag="PWY43_000396"
     CDS             17513..17944
                     /locus_tag="PWY43_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor domain-containing protein"
                     /protein_id="HDL2135309.1"
                     /translation="MAEQIVPSNEAVLKAGHDTLVISTTDLQGVITYANDLFCKLTGF
                     TREELIGQPHNIVRHPAVPKEVYKDMWDTIQAGKIWTGIVPNCGKGGVVYVVDTTVQP
                     MFDETGKITGYISVRRVINDLMTNFEAVESAKEIFDDFYGE"
     gene            18015..19571
                     /locus_tag="PWY43_000397"
     CDS             18015..19571
                     /locus_tag="PWY43_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HDL2135310.1"
                     /translation="MSLIPHIHIAYGSESGNAEKLAQQLAIQPFLLPYSPTLSTLNET
                     DLSQLSAESLLLIVTSSFGDGEPPENADEFAEKLENLTACNLQYAIFGLGDITYDKFC
                     GYSKQLDSLLQQKQAKAVIARVDADLNYQEIFKQWLPLVEQALHSTNKETIQHSLSVQ
                     AYGEEMAYQAEMLEIKHLANSSPAVYHIRLSLKESGIFYQAGDLIYVKVQQPDRLLNE
                     YAEWFNDQNAVALLRHKELRLLSKNVLRDIQKICGSNELKDLTKISNKKALEQYVYGR
                     DLLDVLRDFDPNKSVTLADLEPILSNLAARAYSISSCGKTHPDYVDLCIRHVSYHLNG
                     RDYQGTASGYLSELQAGDFVSIFANANPNFRLPETLTAPVVMIGSGTGIAPHIAFLQA
                     LEQQYQGVESYLFFGERYRDKDFLYQTELENYLAKGTLTQLFTAFSRDQAEKFYVQDA
                     LSQQAELVWHLIQQGAYFYICGSKAMSKAIDAELIKIADQIGGEPYIDDFNNIIAKLV
                     AEGRLMRDVY"
     gene            complement(19629..19886)
                     /gene="rpsQ"
                     /locus_tag="PWY43_000398"
     CDS             complement(19629..19886)
                     /gene="rpsQ"
                     /locus_tag="PWY43_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL2135311.1"
                     /translation="MTDKIRTVQGKVVSDKMDKSFVVAIERTVKHPLYGKFIRRTTKL
                     HVHDENNEAKLGDVVEIKECRPISKTKSWTLVRVVEKAVVA"
     gene            complement(19886..20077)
                     /gene="rpmC"
                     /locus_tag="PWY43_000399"
     CDS             complement(19886..20077)
                     /gene="rpmC"
                     /locus_tag="PWY43_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL2135312.1"
                     /translation="MKAQELRNKSVEELNGELVNLLGEQFKLRMQAATGQLQQTHQLK
                     QVRRSIAQVKTVLTEKAGE"
     gene            complement(20077..20487)
                     /gene="rplP"
                     /locus_tag="PWY43_000400"
     CDS             complement(20077..20487)
                     /gene="rplP"
                     /locus_tag="PWY43_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL2135313.1"
                     /translation="MLQPKRTKFRKVHKGRNRGIAGGTEVSFGSFGLKAIGRGRLTAR
                     QIEAARRAMSRAVKRQGKIWIRVFPDKPITEKPLEVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVSEEVARNAFALAAAKLPFKTTFVTKTVM"
     gene            complement(20501..21208)
                     /gene="rpsC"
                     /locus_tag="PWY43_000401"
     CDS             complement(20501..21208)
                     /gene="rpsC"
                     /locus_tag="PWY43_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL2135314.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTQDFADNLDGDFKVRKFLN
                     KELANASVSRITIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRNAVAKIAGVPAQIN
                     IAEVKKPELDAKLVADSIASQLERRVMFRRAMKKAVQNAMKLGAKGIKVEVSGRLGGA
                     EIARSEWYREGRVPLHTLRADIDYSTAEAHTTYGVIGVKVWIFKGEILGGMAAVIESE
                     KEPAAQPKKAPRGKGRK"
     gene            complement(21228..21560)
                     /gene="rplV"
                     /locus_tag="PWY43_000402"
     CDS             complement(21228..21560)
                     /gene="rplV"
                     /locus_tag="PWY43_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL2135315.1"
                     /translation="METIAKHRYARTSAQKARLVADLIRGKKVSVALEILTFTNKKAA
                     TLVKKVLESAIANAEHNDGADVDDLKVAKIFVDEGPSMKRVMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(21571..21846)
                     /gene="rpsS"
                     /locus_tag="PWY43_000403"
     CDS             complement(21571..21846)
                     /gene="rpsS"
                     /locus_tag="PWY43_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL2135316.1"
                     /translation="MPRSLKKGPFLDLHLLKKVEKAVESGDKKPIKTWSRRSMIIPSM
                     IGLTIAVHNGRQHVPVYVSDEMIGHKLGEFAPTRTYRGHAADKKAKK"
     gene            complement(21877..22698)
                     /gene="rplB"
                     /locus_tag="PWY43_000404"
     CDS             complement(21877..22698)
                     /gene="rplB"
                     /locus_tag="PWY43_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL2135317.1"
                     /translation="MAIVKCKPTSAGRRHVVKVVNAELHKGKPFAALLDTKSKTGGRN
                     NLGRITTRHIGGGHKQHYRLIDFKRNKLDIPGVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLTAGDQIQAGASAPIKVGNALPMRNIPVGTTVHNVELKPGKGGQIARSAGA
                     YVQIIAREGNYVTLRLRSGEMRKVLAECTATIGEVGNSEHMLRVLGKAGANRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRHNKRTDKFIVRRRGK"
     gene            complement(22717..23022)
                     /gene="rplW"
                     /locus_tag="PWY43_000405"
     CDS             complement(22717..23022)
                     /gene="rplW"
                     /locus_tag="PWY43_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL2135318.1"
                     /translation="MIQQERLLNVLKAPHISEKATNNAEKGNTIVFKVALDANKVEIA
                     NAVEELFEVKVDSVRTVVVKGKTKRRGAKVGRRSDWKKAYVTLQEGQSLDFVEGAAE"
     gene            complement(23019..23621)
                     /gene="rplD"
                     /locus_tag="PWY43_000406"
     CDS             complement(23019..23621)
                     /gene="rplD"
                     /locus_tag="PWY43_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL2135319.1"
                     /translation="MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGSRA
                     QKTRAEVSGSGKKPWRQKGTGRARSGDIKSPIWRSGGVTFAAKPQDHSQKVNKKMYRG
                     AIKSILSELVRQDRLVVVEKFEIDAPKTKVLAQKLKDMALNDALIITANVDENLFLAA
                     RNLYKVDVRDVQGIDPVSLIAFDKVVVTVDAVKQIEEMLA"
     gene            complement(23637..24266)
                     /gene="rplC"
                     /locus_tag="PWY43_000407"
     CDS             complement(23637..24266)
                     /gene="rplC"
                     /locus_tag="PWY43_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL2135320.1"
                     /translation="MIGLIGRKVGMTRVFTEDGVSIPVTVIEIEANRVTQVKTLENDG
                     YSAIQVTTGSKKASRVTKPEAGHFVKAGVEAGRGLWEFRTTEGEEFTLGQEINVDIFT
                     DVKKVDVTGTSKGKGFQGGVKRWNFRTQDATHGNSLSHRVLGSIGQNQTPGRVFKGKK
                     MAGHLGAERVTVQSLEVVRVDVERKLLLVKGAVPGATNGDVIVKPAVKA"
     gene            complement(24292..24603)
                     /gene="rpsJ"
                     /locus_tag="PWY43_000408"
     CDS             complement(24292..24603)
                     /gene="rpsJ"
                     /locus_tag="PWY43_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL2135321.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQSTAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHKRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            25047..25688
                     /locus_tag="PWY43_000409"
     CDS             25047..25688
                     /locus_tag="PWY43_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HDL2135322.1"
                     /translation="MQTINEIKEEIYPIFEPIIGTIRLKTYFSYYAVFKDGLMIALYQ
                     NGATYLRIAKKDIEYIRQYPETCILCDEKIGIQSRKFHYIPQVLIDDTPKFSSLLFGI
                     IEELRTEKYKISQKRATQIRNLPNMNLKLERMLKKIGIHSIHDFTQEGYMSTFIKLIL
                     QGFDVTEDLLFKLNGALNHQYIYTFTDQQKRELMQEANQALYNSGLRKRFNAL"
     gene            25849..27243
                     /gene="fumC"
                     /locus_tag="PWY43_000410"
     CDS             25849..27243
                     /gene="fumC"
                     /locus_tag="PWY43_000410"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDL2135323.1"
                     /translation="MEFRIEKDTMGEVQVPADKYWAAQTERSRNNFKIGPEASMPAEI
                     IEAFGYLKKAAAYANHDLGVLAVEKRDLIAKACDEILANKLDDQFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTYPTAMHIAAYKKVVEHT
                     LPCVKRLQKTLAEKAEAFKDVVKIGRTHLMDATPLTLGQEFSAYAAQLAFGIKALENT
                     LPHLRELALGGTAVGTGLNTPQGYDVKVAEYIAKFTGLPFVTAQNKFEALATHDAIVE
                     THGALKQLAVSLYKIANDVRLLASGPRSGIGEILIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDVTISFAGTQGHFQLNVYKPVMAYNFLQSAQLLGDACVSFDEHCAVGIE
                     PNYPRIKQQLDQSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQF
                     DEWVRPEDMVGSLK"
     gene            27370..27480
                     /locus_tag="PWY43_000411"
     CDS             27370..27480
                     /locus_tag="PWY43_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2135324.1"
                     /translation="MKKLILIALVTTLGLSACGVKGPLYFPEQAPKHEQK"
     gene            27585..28835
                     /gene="lysA"
                     /locus_tag="PWY43_000412"
     CDS             27585..28835
                     /gene="lysA"
                     /locus_tag="PWY43_000412"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL2135325.1"
                     /translation="MNHFEYKNNQLFAEEVAIADIIEQYGTPAYVYSRSTLERHLHAF
                     NNAFGSHPHLVCFAVKSNSNIALLNVMARLGSGFDIVSQGELERVLAAGGEASKVVFS
                     GVAKSHSEIKRALEVGIRCFNIESVSELHRINQVAGELGKIAPISLRVNPDVDAHTHP
                     YISTGLKENKFGVSVTQAREVYRLAKTLPNVKITGMDCHIGSQLTELQPFLDATDRLI
                     ILMEQLREDGIELHHLDLGGGLGVPYNGEEPPHPTEYAKALLEKLKGYENLEIIIEPG
                     RAITANAGILVTKVEYLKQNEDRNFAIVDTGMNDMIRPALYEAYMQITEVDKTLNRDK
                     AVYDVVGPICETSDFLGKQRELAISEGDLIAIRSAGAYGAAMSSTYNSRPQAAEIMVD
                     GNQAHLIKARATFADLWRLEKLLP"
     gene            28849..29478
                     /locus_tag="PWY43_000413"
     CDS             28849..29478
                     /locus_tag="PWY43_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HDL2135326.1"
                     /translation="MKLYGLTGACSFVPHVALEWVKLRANQDYAFQAVSREFIKSAEY
                     LALNPRGNVPLLVDGDLALTQNQAIVHYLDELYPEAKLFGSKTVRDKAKAARWLAFFN
                     SDVHKSFVPLFRLPSYAEGNETLTKTIRQQSAEQILEQLAFANAHLENHIFFGEEISV
                     ADAYLYIMLNWCRLLGLDFSHLSQLSAFMQRVEADQGVDNVREQEGLKG"
     gene            29549..30994
                     /gene="tldD"
                     /locus_tag="PWY43_000414"
     CDS             29549..30994
                     /gene="tldD"
                     /locus_tag="PWY43_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDL2135327.1"
                     /translation="MLNQVSNSLLAPSGLELSHLTQVLDHFSGRHIDYADLYFQLSQD
                     ESWSLEDSIIKEGGFYIDRGFGVRAISGEKTGFAYADQITLKQLEQCAAAARSISPTS
                     GQLSVKNFKKTTACSRYPAINPLETISREQKVELLHLVDKTARMADPRVIQVNANLSA
                     VYEEMLVAATDGTLAADIRPLVRLSVSVLVEQNGKRERGSAGAGGRFGLDWFLAPSET
                     GFSRAESLTKEAVRQALVNLTAIPAPAGTMPIVLGAGWPGILLHEAVGHGLEGDFNRK
                     ESSLFSGKIGQMVTSELCTIVDDGTVPNMRGSITVDDEGVPSQRNALIENGILKGYMQ
                     DKLNARLMGVAPTGNGRRESYAHLPMPRMTNTYLTEGNHEFEEMIESVEYGLYAPHFS
                     GGQVDITSGKFVFSTAEAYLIEKGKITKPVTGATLIGSGIEAMQQVSMVGKKMELDPG
                     IGTCGKEGQSVPVGVGQPTVKLDKITVGGRG"
     gene            31058..31939
                     /gene="prmA"
                     /locus_tag="PWY43_000415"
     CDS             31058..31939
                     /gene="prmA"
                     /locus_tag="PWY43_000415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL2135328.1"
                     /translation="MAWVQIRLNSTDKQAEEISDFLTEIGAVSVTFMDSQDTPIFEPL
                     PGETRLWGNTDVVGLFDAETDMNLIVEALKTSKLVDENFAHKIEQIEDKDWEREWMDN
                     FHPMQFGKRLWICPSWREVPDPNAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLEGK
                     TVIDFGCGSGILAIAALKLGAKQAIGIDIDPQAILASQNNAEANGVAEKLQLFLAKDQ
                     PRELQADVVVANILAGPLKELAPNIITLVKPNGNLGLSGILATQAESVCEAYSEAFTL
                     DPVVEKEEWCCITGRKI"
     gene            complement(32114..32189)
                     /locus_tag="PWY43_000416"
     tRNA            complement(32114..32189)
                     /locus_tag="PWY43_000416"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32154..32156),aa:Trp,seq:cca)
     gene            complement(32256..32332)
                     /locus_tag="PWY43_000417"
     tRNA            complement(32256..32332)
                     /locus_tag="PWY43_000417"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32296..32298),aa:Asp,seq:gtc)
     gene            complement(32343..32458)
                     /gene="rrf"
                     /locus_tag="PWY43_000418"
     rRNA            complement(32343..32458)
                     /gene="rrf"
                     /locus_tag="PWY43_000418"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         9449 a         7210 c         6528 g         9333 t
ORIGIN      
        1 atacatttaa gtttgaacct aaatctgaca ctagcgtact ttgtgctgaa gcaacgtaag
       61 ctctcttaat cgtagaataa ccctggttga caactgtacc ctcgttattt acgccatttt
      121 ctaacgtggt attattgaca accaatgtac cacttggaga tacgtttact ttagacacat
      181 attttttacc gttaatagtg acagcacctt cattgagtgt tgtatcccct tcaaaagtac
      241 tagcacccga taaaattaat tcgccttggc catttttcac caagcctgca tcaccgttaa
      301 tattattgga aaactcatag cttccatttg gaatatttac agtcacgtta tcggctaaag
      361 cgagtcgttt atcaaaacgc gcagggccat tagtggcttt ttcaatatta agtaaacccc
      421 atccatatac atcatcaact ccaggagcgc cgatatcggt tgccgtcgag agaatagatt
      481 gtttgataag gttaccatcc atccaaggat atttttgttt cacgagagct gctgtacccg
      541 taacggctgg agctgcaaag gatgaaccac tagcaataat gtttctacct cctaaattaa
      601 agatgtagtc acccattgcg gtaacggtcc aattttttgc cacaccggct tgagaatatg
      661 gcaataaatt gctccattca aagttaccca atccctgatt agttctggtt gctaccgcaa
      721 caacagaaat ccaacctttt tccaagtcag cttcataatg aggtaaccca gcttggagtg
      781 atggatcaag atcaccacgg ttattccccg cagcccagac aaataagccc ccctctttta
      841 ctttatcctt atagaattgc aacacattac catcaaattg gtgtaaatag taatagctag
      901 aatttctatt gttattaaaa taagtaagag gctttggaat accgtatgat tggttaaaaa
      961 tacgggctcc cctattccat aagtcataat aaattggagc tgttgcggat aatcctgtac
     1021 ccgcatttgt tgttttggta atatccgcaa gtaataacgt tgacttagac gctactccat
     1081 tgcgagagtt ccctccccca accatttctg cgacctcaat accatgtgtt gcagaagccg
     1141 gggcagtacg ccctgtttca ataataacag cgcgattacc aaattttttg agtatatcgt
     1201 cctgcattaa atagttttta aaaccactat ctaagatacc gactttaaca ccttcaccag
     1261 ttaacttaac attttgtttg ttattaggat catcaatgtt gtaaccacga gagcggtcat
     1321 tccatcgaat acctacattg ggtacaccaa cagctacagg ttcaggatga agatttaccg
     1381 gtgttgtagg cctaatgata actggacggg tgtcatcaat tggcgattca atgacaggtt
     1441 tgttatctgt aattggatta ggctgtgtat cagtgatggg tttattatca actattggtt
     1501 ggactggcgg atccataatt gggttggcat caataaccgg ttgagttggc gtatctacaa
     1561 cgggtttatt ggcaatcggt gtcggtttat ttacaattgg tttgttcaca gcaggagcat
     1621 aagtggtacg aacggttgtt ctcccattat gcgttgtata aacttttgta actgtaatgc
     1681 cattaactgt tgtcgaaaca gttctggttg ttacacctgc attgctacct ggataatacc
     1741 acgacgaata accattacgg ctattcgatg tatagtagcc cgaataaatc ggtgcatatt
     1801 tatagtaact ataataacta tccgtggcgg aaggataata tcttgttcta cttgcaagct
     1861 gatctgaaag ttcagttgcg ttaacctctg gcgataaggc taatattgtt gctattgcaa
     1921 caaaataatt tctatttttc accttttggt ttccttctaa taaatacaaa gatactcttg
     1981 tttatttcaa atctactacg gatccttaat agacttgtaa aaacaagagg cagagattag
     2041 aacaaaaatc aaaattaaaa tcaagaaaag gaaaatggta attatttgtg gatctatcca
     2101 taaactcaaa cagactgagg agctattccg attatagaaa gcccctcgaa agctgttgat
     2161 aatttggaaa ataattttta ggttattagc tattgcccat aataagcatt tgcaccatgc
     2221 ttacgcagat aatgcttatc taataattcc tgttgcattg ggccaatttg tgggcgaatt
     2281 tgatggctaa taaaattcat gtaagccacc tcttctaata ccacagaatt atgcacagca
     2341 tcgtgtccat cttttcccca ggtaaatgga ccgtgtgaat gtaccaacac acctggaact
     2401 gctttcggtt caatacctcg agttcggaaa gtttcgacaa tcacctttcc ggtttccagc
     2461 tcataactgc cggcaatttc ctctgcggtc attttgcgag tacaaggaat cgcaccgtag
     2521 aaataatcgc cttgagtggt gccaagtgcc aataaatcac gccctgcttg tgcccaacct
     2581 acagcattgc gagagtgagt gtgtaccaca ccgccaatat cagtgaattg gcgataaagt
     2641 tctaagtgcg tttctgtatc agaagacggt tttttatctc cgtaaactcg attaccttgt
     2701 aaatcaacca atacaatgtc ttccaccgtc atcgcatcgt attctacgcc ggaaggtttg
     2761 atggcgacta aaccggtctc acggtcgatt tcacttacgt taccccaagt gaaagttacc
     2821 aagccatact tcggtaattc taaattggct tttaacacac gctctcttaa ggcttgtaat
     2881 gcaggggtta attcagttaa ttgcgtcata gggtaaatcc tccttcttgc attttttgtt
     2941 cgatccaacg gcgggcgttg atgatttcag taatcggctc ttcggctttt tccgtccaca
     3001 tttcaattaa gaatgcgcca cggtaattca gctcggcaag ggttttaaaa caagcggcga
     3061 aatctacgca accgtcaccg aatggtacgt cacggaactg acctttgcag gtttcagtca
     3121 ctttgtaagt gtcttttaag tgaatagctg agattttgtc gatgccgagt ttcagctctt
     3181 ccgctacgtt gtcgttccac gctgagaggt tgcctaaatc cggatacacg gtgaaccacg
     3241 gtgatttgat gatctcgtcc cattttttcc agcgggaaat cgaactcata aattgggtgt
     3301 ccataatctc taccgcaatg gtgacttggt tgcttgccgc cagctctacc gcccattcta
     3361 aaccttcttg gaagcgttga atcgtgcctt cgtcctgctc ttcgtaataa acgtcatagc
     3421 cggcaagctg aatggtgcga ataccgagat ctaccgccag ttgaatggct ttttccataa
     3481 tttcataggc tttttggcgg gtcgcttcat cacaagaacc aaacgggaaa cggcgatgac
     3541 cggacaagca cattgacgga atggtcacgc cggtgttgat gatggttttc accagcttgg
     3601 cacgctcttt tttgctccaa tcaaggcggg ctaagcgttc gtccgtttcg tcaatcgaca
     3661 tttccacaaa gtcaaaaccg cagactttgg cgatagaaag gcggtcttgc cacgaaatgt
     3721 ttttcggcaa ggctttttcg taaataccaa gtttatgttt tctcatagtt ttcccttgaa
     3781 catcataagt aaaattttga ttaaatttga ccgcttgtac aggctaagcc aagataatat
     3841 ccaagcctct atttcgcaat tcttggataa ttgtcggatc agcttctttg ccggtaatca
     3901 ataaatcaat tttatcgaat tctttaaaca gcataccgac ttttttaccg agcttggtgc
     3961 tatccaataa caccacataa cgttctgcgt gctttgccat ttgttgctcg gcattggcaa
     4021 taatcatatc ggttttaaat aatccttcgc tggttaagcc tttgccactg gtaaacattg
     4081 tggtagcggc ataagtcatc tcattttggg tgttaattga gagcatcact tttttatgtt
     4141 tattatattg cccacccata atcactacat catcgtggca acctgcaatc aactggtttg
     4201 caagcggtag ataattggta ataatttgca attttctacc acataatact tcaccgagca
     4261 tttccattgt ggaaccacac gtcagaataa gactttcccc atctttacat aaacgaccgg
     4321 cggcttccgc tattctacgt ttctcctgcg cattgttagt tattttgtgt gctgtaaaaa
     4381 ctgtagaagc tagaattgct tctgctccgt ttctaacctt ttttaattta ccttgaccat
     4441 ctaatttact gatatcacgt cttgctgttg ccggagaaat atttaacaac tgcataatat
     4501 ctgctgttga aagtgctttc cgttcttcca acagggctaa taactctttg tgtctataac
     4561 tttcgttcac tactttatcc cattactaat cactactgct ccataatgaa cttgctaaaa
     4621 cgccactctc cagtgagagt ggcgactgct cacctatagc atggatttaa attgaatatt
     4681 tggctcttgc tcgaagcagt catcaaagcc gcgagggtga tgatattcaa ttaagtcttt
     4741 atcacgtggg taaacatatt tgccgcccac ttcccaaata aatgggtgga attggtattg
     4801 taagcgatct ttacgcatac gccataaatc aataatttct tgcgtacttg ccataaagtt
     4861 tgtccaaata tcatggtgaa ctggaataat cactttacag cgtaagcatt ccgccatacg
     4921 taataaatca actgaagtca ttttatccgc aataccaacc gggttttcgc catagttatt
     4981 cagtgctacg tcaatatcaa attgcttacc gtgtttagcg aattggattg agtagtgaga
     5041 atccgcaccg tgataaatcg taccgcccgg ggttttgaac acatagttca cagctttgcg
     5101 tcccatttct tcatctgatg ggcataagcc tgcaagttct ccgccacgtt cttctgcacc
     5161 ttctaccggt aaggtcacta aacaagtacg gtcaaatgaa tctagggcgt gaatttctac
     5221 atcttttaat ttcacgacat cgcccggttt taccacaatg atacgctctt ccggcacgcc
     5281 ccattttttc cataattctg cacagtgcca cgggccgaca aatttaacgt ggtctaattt
     5341 cggattattg caaatagccg ctaccacgtt tacatcaata tggtctgagt gatagtgaga
     5401 agcgagaatg aagtccactt cgttaatcgc aaacggatct aacaccatcg gttgagcccg
     5461 caaattaggc tggagcttac gcacccctgc catatttgcc atttgatgac cacgcaccat
     5521 atcttttact ttcttggtgg atttaccacg accgcaccat aaatccatac agatattcgc
     5581 accagctggg gttttaatcc acacaccgac acagcctagc caccacattg caaaattgcc
     5641 ttccggtaca acttcttctt caatttcttc attcaaccaa gtaccccatt cagggaaagt
     5701 tgaaaggatc cagctttcac gagtgatttc ttgaattttg ctcataatag tctcctgatt
     5761 tacgctaaaa taagaacaat gcaatcaaaa ccaatcataa acaatcacaa aaatttgtac
     5821 atctttttta ttcaaaaatg tgacctatgt ctaatttttt atgatttact ggtaaaaatt
     5881 tattttaata acttgattta aataccattt atttagcata tatgagctaa tttaaagaat
     5941 aatctttaat aatcacgaat aaatatattt ttcttcctcc atgacgagat tataaaaata
     6001 aaatgtgatc tcgctcacaa ataattaaaa ataatctttt ttaatctttt tctagcgttt
     6061 atcattaatt ccgacaactt ctctgaagtt ttttagttct attaatctct caacaaagag
     6121 gccccctatg gactttttaa ccgaaattct ccttttctta aaggatcaag tgctgaataa
     6181 agcacccttc ttactaggta tcgtagcttg cttaggttac attcttctta aaaaagacac
     6241 ttctacgatt attaaaggca caattaaaac aattgtcggt tttatgattg tacaagccgg
     6301 ttcaggtttc ttagtaacca actttaagcc ggttattgat ggcttagcca aatatcacca
     6361 acttaccggt gcagtaattg acccttacac cagtatgcaa tcgaccattc aaaccatggg
     6421 cgagaactac gcttgggttg gttatgccgt aattggggcg ctgttcctca atattctatt
     6481 agtggtgttc cgccgtttaa ccggtatccg taccattatg ctcaccggtc acattatgtt
     6541 ccaacaagca ggcttggttg cggtgttcta tatgatcatc ggagcgtcaa tgtgggagac
     6601 cattatctac acctcagtgt tgatggctct ttattggggt atttcgtcta atattatgta
     6661 taaaccaacc caagcggtaa caggcggtgc aggcttctca attggtcacc aacaacaaat
     6721 tgcttcttgg attgcagtca aactcgcacc aaaacttggc gataaagacg atactgtaga
     6781 taaaatgaaa ttgcctaact ggctcaatat tttccacgac agtatttctg caaccgcatt
     6841 agtaatgacc atcttttttg gaatcatctt actttcattc ggtttagata acctcaaaga
     6901 acttgctggc aaaaccaact ggtttatgta cattcttgaa atgggcttga aatttgcggt
     6961 ggcaattcaa atcattgtaa ccggcgtacg tatgtttgta gctgagcttt cagaagcctt
     7021 caaaggtatt gctgaacgct taattccaaa ctcagtatta gcgattgact gtgccgctat
     7081 ctacgctttc tcgccaaatg cgatggtatt cggctttatg tggggggcta tcggtcaatt
     7141 tgtggcagta ggcggcttgc ttgcagtagg ctcaccggtg cttattattc cgggctttat
     7201 cccgatgttc ttctctaacg caaccattgg tgtgtttgca aaccaatttg gtggttgaaa
     7261 gcagtaatga aaatctgttt tgtgatgggt attattgaag tgcttggctc agcttgggtt
     7321 attcaaatgc ttgcttcaca aggcacaacc tttaacggtt ggatgggtat ggctgactgg
     7381 gcattgttct tcccaccaat cctacaaggt attatcagca ttccgggctt cttctttgta
     7441 attgtggcag cagcaatggt ttatatgttc ttcgcatcta aacaacttcg tgccgaagaa
     7501 gcagaagcgg cagctcgtgg cattacactt gaacaattag acggctacgg cttagatgaa
     7561 gtaaaagcag aaccaaaagc ggaggcagta gtgcaacaag cggtcgaaaa tgccgaaaat
     7621 tctgcaaacc caatccgcat tctagcggtg tgtggctctg gtcaaggctc atcaatgatg
     7681 atgaaaatga agatcaaagg ctatctcgac aaacgtggta ttccgaatat tatggattcc
     7741 tgtgcgctaa ctgattacaa aggcaaatta agcgaggttg atattattgt ctccagcaaa
     7801 catttagcca acgaaatcga agtaccggaa ggcaaagcgg tattaggtgt acaaaatatg
     7861 ctaaatccaa actcatttgg tgatgaatta gttgagctaa tcaatcagca taatgcaaaa
     7921 taagtaagtt taggggtgaa taatcacccc tatctatgct aaagattgta aaaactcact
     7981 tcatcaaacg aggaattatc caatgaaaaa aatcttatcc accctgttta tgctctccgc
     8041 ggttagcgtt gccgttgcca aagaggtaaa cattaaacta cttggtacct cagacgttca
     8101 cggacgtgtt gtgccgtgga gctacggggc agacgtggaa gacaaatccg gctcattcgc
     8161 ccaaattgca acctatgtca aagaggtgcg tgccaaccac aaaaatgtgt tgctgtttga
     8221 tattggcgat gcgattcaag acaaccaagt cgaagagttc gctaaaacct tagacaaagc
     8281 gaaaaacaat cccgtgccga aagtgctgaa tgcaatgaat tacgacatct tcattctcgg
     8341 caaccacgaa tttaacttcg gaatgccgaa tttagatgcg attttaaaag acattaacgc
     8401 caaaattctc actgccaact tctatcacaa agacggcaaa cgctacgttg agccaactac
     8461 catcattgaa cgtgatggcg taaaacttgg gattatcggc ttaaccaccc caatgtcggc
     8521 tatttttgaa aaagacaccg gtcatttaga cgcaatgaag ttcacttcgc cgattgaaga
     8581 ggcgaaagcc caaattgccg atctcaaagc caaaggcgtg gacgccatta ttgtgctggc
     8641 acatatgggg attgagaacg aaaacaacat tcccgacacc ggcgtggcgg atttagtgaa
     8701 tgcagtggac ggtattgatg taattatcgc cggccatatg cataaagatg tgccgagtga
     8761 aactatcaaa aataccctga ttaccgaacc gcaccgctac ggcacagtgg tttccgaagt
     8821 tgatcttgcc tttgatgtga atgacaaagg cgaagtgaaa ctactttcta aaaccgccaa
     8881 aaccgtaccg gtgaagaaac tcgcaagcga tccggaaatc gaaaaaattt accaaccgta
     8941 tcacaatgaa ctgcgtgcct taaacaacgt caaaatcggt gaaactgcac aagatttaat
     9001 cccacaaggc aaaatcacgg tgtatcgatt gctttctcgc aagacaccgg aatgtcctca
     9061 ttgatccacg atgtgcaaca acattacagc ggtgcagacg tagtgagttt tgcatttgac
     9121 tacgaaaccg tgcgtttaga caaaggcgac atcaagaaaa aagacatcat ctacaactac
     9181 cgctatgccg gtggcgatgt gagcatgtat gagctaaccg gcaaacagct taaacagtat
     9241 atggaatggt cagcggatta cttcgacacc attcaagccg gtgacaaaga ctaccgctac
     9301 aacgctgtgc ggggtaaatc taaatacgtt accttcgacc tattcggcgg cgtaagctac
     9361 aacatcgacc tacgcaatcc aagcggtagc aaaattgtcg atctaaaact ggcaaacggc
     9421 agtctaatca ccgatgacgg caaatacaaa gtgggaatga actcctaccg ctttggtcag
     9481 ctcaccaaaa aaggcggcat ctgggaagga cagtaaatcc ccgtgatttg ggaatccaaa
     9541 gtcgccatgg gggctgaaaa aggcacaatc caaaatatgc tgattgatta catcaccaac
     9601 gtcaaaggcg gaaaaatcga aggcaaatcg cataaccgct gggcgattat cggcttaaat
     9661 taacgaaagg gaaacactat gaacttaaaa caatctctta ttgaaaataa ctctattttg
     9721 ctcaatcaat cagcggcgaa ttgggaagac gcaatcaaaa tcggcactga tttattagtc
     9781 aaagccggta cgattgaacc acgctattac gataatattg taagcaaaat caaagagatg
     9841 gggccttata ttattcttgc accgggcttg gcaatgcctc acgcccgtcc ggaagaaggt
     9901 gtgattaaaa cctctttcgc tttagtgact tttgacaccc cgatttattt tgagggcgaa
     9961 gatgagccgg ttcacgttat gctaaccctt gcagggagtg actctgatca gcatatgcaa
    10021 ggcttggtag aaatcaccca agtgctagat gacccagaga gcgacacagg tgttgattta
    10081 gataaaatcc gccgctgtaa aacggctgaa gaagtgtatg cagtaatcga tcaggcatta
    10141 gcctaatatt ctacaagcgg ttcaaaaaca tcaatatttt gcaaatagag gaatacaatt
    10201 atgtccaaac cattattaca aattgccctt gattctcttt cactggaaaa agcggtggcg
    10261 gatgcaaaac aagcagaaaa ccaagtcgaa attattgaat gcggtactat tcttgctttt
    10321 gctgagggta tgaaagccgt ttccaccttg cgtgcattgc acccaaatca cattattgtg
    10381 tgcgacttaa aaaccaccga tggcggtgca attttagcta aaatggcatt tgaagccggt
    10441 gccgactggc tcacggtttc ggctgcggct caccctgcaa ccaaagcggc ttgcaaaaaa
    10501 gtggcggacg aatttaacgc tgctcacccg gagctaaaag tgaaaaaaga gattcaaatt
    10561 gaaatctacg gcaactggac ggtggaaaaa gatgccaaag agtgggtaga attaggggta
    10621 acccaagcga tttaccaccg ctcccgtgat gcggaactag caggtaaagg ttggacaacg
    10681 gaagatgttg agttaatgaa acagctttct gacttaggca ttgaattatc tatcaccggc
    10741 ggtatcgtac cggaagatat tcacctgttc aaagagatca aaaacgccaa agcctttatt
    10801 gcaggccgtg ctttagtcgg tgaaaaaggc aaacaaactg ccgaagccat tcgtgtggag
    10861 attgataaat actggaacta atccggcatt gaaacccaat aaaaatggcg ttctctcaac
    10921 cggaacgcca ttttttatct cattttttct attcttgcga ggccgatatt ttccacaatc
    10981 tcgctacatt ttcggcagtt gaaatcaggt tctctttgcc tgattttaac accgcttcta
    11041 aattgctgac ctgtcgaata atcggaaata ccgcatcaat gccgtgttgg tacaccacag
    11101 ggtaatcttc tttcaatgag ccaactatcg caatcaccgg cttgccaaac tgcttagcga
    11161 ctttcgccac cccaatcggg gttttaccgg cgatagattg agcgtccatt cgcccttccc
    11221 ctgtcaccac caaatccgca tcggcaagtt tctcagcaag atcggttgcc tcaagaataa
    11281 tttgcacgcc ggaacgtaat gttacgttag gcagtagcag taaaccaccg cccattccac
    11341 cggctgcacc ggcaccggct ttctcggcaa tctcaatgcc taattgggat tttacggttt
    11401 cggcaaaatg caacagagcg tggtcgagcg ttttcaccat ctctgctgtg gctcctttct
    11461 gtggcccaaa aattgccgaa gcccctcgct cgccacacag cggattatct acatcgcaag
    11521 caacctcaaa ttccacttgc tttaagcgtg aatccaaacc gcttaaatca atctgctgaa
    11581 tttggcttaa tgcctcgcca ccaaagccga tttcccgctg ttcggcattt agaaatttcg
    11641 ctcctaaggc ttgtagcatt ccgacaccgc catcgttagt cgcactgccg ccaatgccga
    11701 gaatgatttt ttccacccct aaatctaagg cggctttaat taactgcccc gtgccgtagc
    11761 tggtggtaac cagcggattt cgttgctcca ttggcactaa atgcaagcca cttgccgccg
    11821 ccatttcaat aaaggcggtt ttttgatccc ccgacaagcc aaaaaagccc tccacctcat
    11881 ttgcaagcgg tgcaattacg ctgatttttt gcaaactgcc gtgagtggca tcaaccagcg
    11941 actgtaccga gccttcgcca ccgtctgcca tcggtacgag ctgatactcc gcctcaggga
    12001 aaacacgggc aaaaccctgt tgaattgctt gagccacttc taatgcggtc aaactctctt
    12061 taaatgaatc cggtgcaatc acaattttca tcgtcaatcc ttagagtaaa atcaatgaaa
    12121 gcagataaac cgtgagcata cccaccacac ccataatgaa agttagggta gtttgcgtac
    12181 gatagccttg ttgcggagtc agtttagtaa agtttgttac cacccagtag tagctgtcat
    12241 tggcgtggga aacgcacatt gagccagccg aaatcgccat tacggttaag gctgccgcca
    12301 tttcactggt tagccctaag gcgttcatta atgagtccgg tgcgttgaac attcccataa
    12361 tggaggcggt ggtgataatt gccaccgttg agctgccttg tgcggttttt aaaattgccg
    12421 aaatcaggaa cgggaagaaa atccctgctg tactgataat atgagcgttt tgcttgatgt
    12481 aatccacaaa gccggcttga gtaatcacgt tacctagcac accaccggcg gcggtaataa
    12541 agagaattgg gccgactaat ttcagggtat cattggttaa gctgtcaaat tccgccattt
    12601 taccgcttga aaccagtagg aaaatagcga agatcacccc aattgctaac gcaataatcg
    12661 ggttgccgac aaattgtagg aatacgccga aatcgccact tacaccggca attttggcaa
    12721 tcgagccgat agccattaag ataatcggca taaaaatcgg ggcaaggctt aaaaacccac
    12781 ttggtaattt gccgtattgt ttgatcagat cttcatagct ttggctgatt acttgatccg
    12841 cttgagcttc ttccatcacg ctgacttgtt tgccaacata tttggcaaag aagtaactgg
    12901 caatcaacac aggaatcgaa accacaaagc ccattccgat cacaagcagg agattatggc
    12961 ttaaaccaac agcaccggct gctgcaatcg ggcccggtgt cggaggaata aagacgtggg
    13021 aggcatataa accggcactt aacgccaccg ccattcccac cggattggca agaatttttt
    13081 tgcgaatagc ttcacgaatc gggtttaaca ccacaaagcc gctatcacag aacactggga
    13141 taccaaccac ccagcccata atcaacattg ccagttcagg gcgtttttgc cccaccactt
    13201 ttaccaccat atcggctaat ttgagggctg caccggtttt ttctaaaatc gtgccgataa
    13261 tcgccccaaa aataatcacg ataccgatac ttttgaaggt attactaaaa ccttcgccca
    13321 caatagttgg aattttggca agcggtaaac ctgccactaa tgcaagggct atcgaaatcg
    13381 acattaaggc taagaacggg tgaactttcc acttcgcaat catataaatc attatgatga
    13441 tagcgaccag aaagatcaga ataagtgcaa tacctgacat aataagtctc ctcattgtag
    13501 aaaaatgaac ttattattcg gtatttattg ctaaaaagat ttagataaat ttgcaaaaaa
    13561 tttgttaaaa acgaccgctt gtttgtatat ttttacaatt tttacgacag aaccgctcca
    13621 agatacagca caaatttttg ctctatgtta ttgaaagata aaccagttat ctgctcaatc
    13681 ttctctaaac ggtaacgtag cgtatttggg tgaataaaca attttttggc agcgtgatcc
    13741 agatcacaat ttgcgaaaaa atagtaacgc agacttttca gtaacacctg ttttttatcg
    13801 gaaaccagta aagcttggaa cggcgagagc aaggttttcg cttcccaaga gtggctgaaa
    13861 ctgtgcaaga gggccggcag tttaaaatct tcaaaatgaa taatctgctt acggctttgg
    13921 atctgctcgg cataattgag ggtatggcag gcggtttggt aggaaatatg ggtttgcgat
    13981 aaattttcga ccgccagccc aaccaccgtt ttgaaggaaa tatccggatc gttaagtttg
    14041 tttagtgcat tgcggattaa cgatttttcg ttggataaat gattcagcaa ggcaatttta
    14101 tctaacccaa tcacggcggt gagtaggctc gggaaataat gttcgaaaaa atcaatcagg
    14161 cgttggagtt tttccgcagt agagccgtga attttaatca ccatcaccga cagcggtttt
    14221 gcaaaatctt gcccgaaaaa gaccgcttgt tgctggattt ctgctgtgct taggctacct
    14281 ttgagcaagg cccgcacaaa ctcttcccga tagcggcgtt gccaacgttc ctgttccaac
    14341 tcaaacgctt gctgcataat cagctccgcc gccattttca ccaattgagc atattgccga
    14401 acttgttgtg gctgccctga aatccccact acaccaatgg ttgagcctaa atagtgaagc
    14461 ggtaagttaa tgcccgcttt cacttcatcg caccactgtt ttgcaagatt ttcatcaatt
    14521 tcgaccgctt gatcgttgcg taacgcaatc accgctcctg tgtgtcgttg cccgatacga
    14581 ccgacatcgc cggaagcaat aatcacccct tcactatcca tcacattgac ggagttggga
    14641 ataatctgca tcgtgcgctt gacgatgcgg tcggcaatcg cttggttgag tttcattata
    14701 tgattcctac tcattcccaa tactatgaaa aatccctctt tcgagggatt tgcaaattat
    14761 ctacgcttcc acttcctgag aatagatcat agcgtgaagt tattttgcca cagtcgcttg
    14821 aattaatttt tccgctttgg caaggttgtt ttctacatca gcataatcgg tattagcgtg
    14881 agccaccact tgatcggcac gatttaacat tacttcgacc ttgcccttgt tcggttcggt
    14941 aattaacact tttaacggta attgcaacgc taacatcgga tctttcacca ttaatggcgt
    15001 accggctttc ggtgtgccgt aaaccaatac ggttgccggt tgcatttcta agccggcttg
    15061 ttttgccgcc gcttggtgat cgatttcggc aaaaatcgtc attccttttt cttttaaggt
    15121 gttggttaaa gttgtgactg tagtttggaa atcaaattta ctttcaaata ccaccggttt
    15181 gacatcggca atatctgcca ttgccacgct gctcattgct aatgcagaaa ctgcgactgc
    15241 ttttaagttc atttcttttc tcctcaaata gattaaataa tcccttgttc ttccaacttg
    15301 cctaagaaat aaggcatctg tttacgccaa gtcggccagt catggtcaat atcaaagccc
    15361 cagtagtcaa accaagcatc gatccctttt gcgttaaaag cctcttgtaa tcggcgggtg
    15421 tcctcaatat gctgttcttc ccacgcacct tgtccgactg caatcacata gtgattttgg
    15481 cggtagcggt ctaagaacca atcgtcattt tgaccccata cataatcaat cggcgagtta
    15541 aaataaaccg ctttatcgcc ataaaactca ccactgaaaa agcgagcgtc ataaacaccg
    15601 cttaaggcga tggcaatatc aaacagatcc ggatgacgta aggcaaagtt taccgtatgg
    15661 aaagccccca tactacaacc ggtggcaatc atcgtgccgt tccagtgcga ttcatggcgg
    15721 attaacggca ctagctcgtg aataatataa cggtcgtaag cattatgagc taatgccata
    15781 tcgtgtccgg atttatgttt agataaccaa gattccgcat cgtaagaatc aggcgtgtaa
    15841 aatttgatta agccacgttc aataaaggaa cggcaagcct caatcattcc aaaattacca
    15901 tactcgtttt cattgccgcc ggaggaaggg aaaacaataa ccggtttacc tgcgtgtccg
    15961 taaacgttga acgacatttc acgacctaat tctccactcc agtggcttct tttttcaaaa
    16021 tgcatacttc acctcttact gtttagcttg tgcaaattgt gcaatttcac gcatttgctc
    16081 cattgtttcg gtacgggcta aaatgccttg ttcgcccatc actttggcaa atacgcccgg
    16141 tacgctttgc acgctgataa tattgccggc aaatttctca caaacagctt caatcgaatt
    16201 cacataattt tggtttgcct tgcgggaaat ataaaccacg ttccaagggt gctgaatatc
    16261 ggcatagaat ttgttttctt tcacaatatt ggcaaattca cggaacacat cgaaatcgtt
    16321 ggcgtagttc cacatatcaa tagttaagcc acccggcgga cggcagttga tttcgagtgg
    16381 taacaactct tcttttttgc tccctttaac acagaagaac tcaaaatgga aaaaacgctc
    16441 ttttacgtta aaggctttca cacattgttt gcccatttcc actaattttg gcgaaatttc
    16501 acatgggaca tagtagaaca tatcgccatc ttttgaaacg gtatctaata ccgcttctga
    16561 atattcaagg ctggagtaga aaacgatatt gccctcttta tcggttaaac cgtcaaaggt
    16621 aataatatcg ccgtcaataa actcttccat aatatattca accgttggcg ttttataacc
    16681 gaagaaagct tctaactctt ccgcagtttt gattttgtaa gtatcgcttg ccccaacccc
    16741 tgaatttggc ttgataatca ccgggaattt aagctgtttt gctaatttgc aggcatcttc
    16801 atcatcttta aatactcgcc cttgtgccac tttcaagccg gttttgcgga atacctcttt
    16861 catttgggct ttggttttaa ttgagagcat atcatcgctc ttataaccaa acacattgaa
    16921 atcggtacgc aatttcgcat ctaactccag ccaatattca ttgtgcgatt caatgcggtc
    16981 aattcgcccg tatttatggg caaagtacgc gaccgcacga taaacttgtt cgtaatcttc
    17041 catattatct acacgataat attcagtcag ggaatgacgc agattttcgc tcaatgattc
    17101 ataaggggca tctgcaattc ccaatgtatt aaaaccggct tcacgcaaac gaatggcaaa
    17161 ggtttcaaag ttcgttggga agtgcgggga aatcatcaca aaattaagtg gtctagacat
    17221 agttcttcct ttataaagtt tgcatattat taaaatgatt agaatagatt ttaggctaaa
    17281 tttattaatt ttcaaaatgt attcacacaa gcaataattt ttacaataaa ttaactttta
    17341 taaaaactca atttaacatc agaataaatc gtataaattt agtcaggtat ttttacaaaa
    17401 aaaccatatt tatgatttac attaatattt ccatctatta tcctaaatat gttgtcagta
    17461 tttatactga taaacacata acaaaattca tttatattaa tggagattca acatggctga
    17521 acaaattgtt ccttcaaatg aagctgtttt aaaagcaggc cacgatactt tagttatttc
    17581 aacaactgat ttacaaggtg ttattactta cgctaacgac cttttctgca aattaaccgg
    17641 ttttacccgt gaagaattaa ttggacaacc gcataatatc gtacgccacc cagctgtacc
    17701 aaaagaagtt tataaagata tgtgggatac aatccaagcc ggtaaaatct ggacaggtat
    17761 cgtaccaaac tgcggtaaag gcggtgtagt ttatgtggtt gataccaccg ttcaaccaat
    17821 gtttgatgaa acaggcaaaa ttacaggcta tatcagcgtt cgccgtgtaa ttaatgattt
    17881 aatgaccaac tttgaagcag tagaatcagc aaaagaaatc tttgatgact tctacggtga
    17941 ataattaaaa aacaatgatt gcgtgtaaaa gatgatctct tttacacgca attttttatt
    18001 ttcgaggagt ttgtatgtct ctcattcccc atatccacat tgcttatggt tcggagtctg
    18061 gtaacgcaga aaagcttgcc caacaattag ctatccaacc ctttttactt ccctactcac
    18121 ccacactttc tacattaaac gaaaccgatt taagccaatt atctgccgag agtttattgc
    18181 ttattgtaac cagctccttt ggtgatggcg aaccacctga aaacgccgat gaatttgcag
    18241 aaaaattgga aaatttgacc gcttgtaatc tgcaatatgc gatattcggg ctaggtgata
    18301 ttacttatga taaattctgt ggctatagca aacaactaga ttcattatta caacaaaaac
    18361 aagctaaagc tgtgattgca agggttgatg ccgatttaaa ctatcaagaa atttttaaac
    18421 aatggctacc cctagtggag caggcattac attcaacaaa caaagaaacg atccaacatt
    18481 ccctttctgt tcaagcttac ggcgaggaaa tggcatacca agcagaaatg ttggaaatta
    18541 agcatttggc aaactcaagc cctgcggtgt atcacattcg tttatcatta aaagagagcg
    18601 gtattttcta tcaagcaggg gatttaattt atgtcaaagt gcagcaacca gacagactat
    18661 taaatgaata tgcagaatgg tttaacgatc aaaatgcagt tgcattacta cgccataaag
    18721 aactccgact actaagtaaa aatgtactgc gtgatattca aaaaatttgc ggcagtaatg
    18781 aactcaaaga tttaactaaa atttccaata aaaaagcatt ggaacaatat gtctatgggc
    18841 gggatttatt agacgtatta cgcgattttg acccaaacaa aagcgtgaca ttagccgatt
    18901 tagagccaat tttatctaac ttagccgcac gagcctattc aattagctct tgcggtaaaa
    18961 ctcaccctga ttatgttgat ttatgtatcc gccacgtttc gtatcattta aacggtagag
    19021 attatcaagg gactgcaagt gggtatttat ccgaattaca agcgggcgat tttgtctcga
    19081 tttttgcaaa tgccaatccg aatttccgtt tacccgaaac attaaccgct ccggtggtga
    19141 tgattggctc aggtactggc attgctccgc atatcgcatt tttacaagcg ttagagcagc
    19201 aatatcaggg ggttgaaagc tatttattct tcggcgagcg ttatcgtgat aaagattttc
    19261 tgtatcaaac ggaactggaa aattacttag caaaaggcac cttaactcag ctgtttactg
    19321 ctttttcacg tgatcaggca gagaagtttt atgtgcaaga tgccctatcg caacaagcag
    19381 agttagtttg gcatttaatt cagcaagggg cttatttcta tatttgtggt agcaaagcaa
    19441 tgagtaaagc aatagatgcg gaactcatca aaattgcaga tcaaattgga ggcgagcctt
    19501 atattgatga tttcaataat attattgcaa aattggtcgc agaaggccga ttaatgcgag
    19561 atgtatatta gcaaatgaaa aaaactaaaa acccagcaaa agctgggttt ttatttaatc
    19621 atagttcatt aagcaacaac tgctttctca actacacgaa ctaaagtcca agatttagtt
    19681 tttgaaatag gacgacattc cttaatttca acaacatcac ctaatttagc ttcgttgttt
    19741 tcatcgtgta catgaagttt agtagtacga cggataaact taccgtataa cggatgttta
    19801 actgtacgtt cgatagcaac aacaaatgat ttatccattt tgtcgctaac cactttacct
    19861 tgtactgtac gaattttatc agtcattatt cacccgcctt ttcggttaat acagttttaa
    19921 cttgtgcaat actacgacgc acttgtttta actggtgggt ttgttgaagc tgaccggtgg
    19981 ctgcttgcat acgcaatttg aattgttcac ctaaaaggtt cactaactca ccattcagct
    20041 cttcaacact tttattacgt agttcttgag ctttcattac atcaccgtct tagttacaaa
    20101 tgtggttttg aacggaagtt tagctgccgc taatgcaaac gcattgcgtg caacttcttc
    20161 agaaacacca tccatttcat agagaacttt acccggttgg attaaggcta cccagtactc
    20221 aacgttacct ttacctttac ccatacggac ttctaatggt ttttcagtaa ttggtttgtc
    20281 tgggaacaca cggatccaga ttttaccttg acgtttaacc gcacggctca tcgcacgacg
    20341 agcagcttca atttgacgag ctgttaaacg accacggcca atagctttta aaccaaatga
    20401 accgaagctc acttctgtgc cacccgcgat accacggtta cggcctttgt gtactttacg
    20461 gaattttgtg cgttttggtt gtaacattca gtgattctcc ttatttacga cctttgccac
    20521 gtggcgcctt tttaggctgt gccgctggtt ctttttcaga ttcaatcact gcagccattc
    20581 caccaagaat ttcacctttg aagatccaca cttttacgcc gattacgccg taagtagtgt
    20641 gagcttcagc agtactataa tcgatgtctg cacgaagcgt atgtagaggt acacgacctt
    20701 cacgatacca ttctgagcga gcaatttctg caccacctaa acgaccgcta acttcaactt
    20761 tgataccttt agcacctaat ttcatcgcgt tttgaactgc ttttttcatt gcacgacgga
    20821 acatcacacg acgttcaagt tgagaagcga tgctatctgc gactaatttc gcatctagct
    20881 ctggtttttt cacttcagcg atgttgattt gagctggaac gccagcgatt ttcgcaactg
    20941 cgttgcgtaa tttttcaaca tcttcacctt ttttaccgat aacgataccc gggcgagctg
    21001 tgtgaatagt tacacgaatg ctttttgcag gacgctcaat agtgatgcgt gatacagaag
    21061 cgttcgctaa ctctttgttt aaaaatttac gtactttgaa gtcgccgtct aagttatcag
    21121 cgaaatcttg tgtattcgcg aaccaagtag agttccaagg tttaacaata cctaggcgaa
    21181 taccattagg atgtactttc tgacccattt gttattctcc tacttaatta acgatctgac
    21241 acaaccacag tgatgtggct tgtacgtttt aaaatacgat ctgcacgacc ttttgcacgt
    21301 ggcattacac gtttcatgct tggaccttca tcaacgaaga ttttagcaac tttaagatca
    21361 tcgacatctg caccgtcatt atgctctgcg tttgcaatag ctgactctaa tactttctta
    21421 acaagcgtcg ctgctttttt gttagtgaaa gttaagattt ctaatgctac agaaactttt
    21481 ttaccgcgaa ttaaatcagc aactaagcga gctttttggg cagaagtacg agcgtaacga
    21541 tgtttagcaa tagtttccat cttttacctc ttatttctta gctttcttat ctgcggcgtg
    21601 accgcggtat gtacgagtcg gtgcaaattc acctaattta tgaccgatca tttcgtcgga
    21661 aacataaaca ggaacgtgct gacgaccatt atggactgcg atggtcaaac caatcattga
    21721 tggaatgatc attgaacgac gggaccaagt tttaattggt tttttatccc cgctttccac
    21781 cgccttctct accttcttca acaagtgtag gtcaaggaaa ggacctttct tgagggaacg
    21841 tggcattggt gtttaccttt tagttaatga attaaattat ttgccacgac gacgtacgat
    21901 gaatttatca gtacgtttgt tgtgacgagt tttcttacct ttggtttgaa cgccccaagg
    21961 tgtaacaggg tgtttaccga agttacgacc ttcaccacca ccgtgtgggt gatctaccgg
    22021 gttcattgca gtaccacgaa cagttggacg aatacctctc caacggtttg cacctgcttt
    22081 accaagaacg cgaagcatat gctctgagtt acctacttca ccaatggttg cagtacactc
    22141 agctaatact ttacgcattt cgcctgaacg aagacgtaaa gttacatagt taccttcacg
    22201 ggcaatgatt tgaacgtaag caccagcaga acgtgcaatt tgaccacctt taccaggttt
    22261 taattcaacg ttgtgtacag ttgtacctac tgggatgtta cgcattggta atgcgttacc
    22321 tactttaatc ggagctgaag cacccgcttg aatttgatca ccagcagtta aaccttttgg
    22381 tgctaaaata tagcgacgtt caccatcttt ataaagtact aatgcgatat tcgcagaacg
    22441 atttggatcg tattctaaac gctcaacaac acccggaatg tctaatttat tacgtttaaa
    22501 atcaattaaa cggtagtgtt gtttgtgacc gccaccaatg tgacgagttg tgatacgacc
    22561 taagttgtta cgaccaccag ttttagattt agtatctaaa agtgctgcaa aaggtttacc
    22621 tttgtgaagc tcagcgttta caactttaac aacgtgacga cgaccagcgg aggtcggctt
    22681 acatttaacg atagccattt attttatctc ctctgattac tctgccgcac cttcaacgaa
    22741 gtcaagcgat tgaccttctt gaagagttac ataagctttt ttccagtcac tacgacgacc
    22801 aactttagca ccacggcgtt tagttttacc tttaactact acggtacgaa cagaatcaac
    22861 ttttacttca aacagttctt caactgcatt tgcaatctca actttgtttg catctaatgc
    22921 aactttgaat acgattgtat tgcctttttc tgcgttgttt gtcgcttttt cagagatatg
    22981 tggtgcttta agcacgttta gcaaacgttc ttgttgaatc atgctaacat ctcctcaatt
    23041 tgttttactg catctacggt tacaaccact ttatcgaaag caattaagct aactgggtca
    23101 ataccttgca catcacgcac atcaacttta tataagttgc gtgctgctaa aaataaattc
    23161 tcatctacgt ttgcagtaat gataagagcg tcatttaacg ccatatcttt taatttttgt
    23221 gctaatactt tagtttttgg tgcatcgatt tcgaatttct caacaaccac taaacggtct
    23281 tgacgaacta attcagaaag aatgcttttg attgcaccac ggtacatttt tttgttcact
    23341 ttttggctgt gatcttgtgg tttcgcagca aaagttacac cacccgaacg ccaaattggt
    23401 gatttaatat caccagaacg cgcacggcct gtaccttttt gacgccaagg ttttttacct
    23461 gaaccagaca cttcagcacg agttttttga gcacgagatc cttgacgagc acctgctgca
    23521 taagcaacaa ctacttggtg gattaatgct tcattgaact cacgtccgaa ggtagtttca
    23581 gaaacagtta gtgcgtttgc acctacaact tgtaattcca tctctatctc ctagacttat
    23641 gctttaactg ccggttttac gataacatca ccattagttg caccaggaac ggcaccttta
    23701 actaataata atttacgctc aacatctaca cgaacaactt caagtgattg aacggttaca
    23761 cgctctgcac ctaagtgtcc tgccattttt ttacctttaa acacacgacc tggagtttgg
    23821 ttttgaccaa tagaaccaag tacacgatgt gataaagagt taccgtgagt tgcatcttga
    23881 gtacggaagt tccaacgttt aacaccacct tggaagcctt taccttttga tgtaccagta
    23941 acatcgactt ttttaacatc agtaaagatg tcaacattga tttcttgacc taaagtgaat
    24001 tcttcacctt cagtagtacg aaattcccat aaaccgcgtc cagcttcaac acctgctttc
    24061 acgaaatgac ctgcttctgg tttagttaca cggctagctt ttttagagcc agtagtaact
    24121 tgaattgcag agtagccatc gttttcaagg gttttaactt gagtaacacg gttggcttcg
    24181 atttcgataa cggtaactgg aatagacacg ccgtcttcag tgaaaacgcg ggtcatacca
    24241 actttacgac cgattaaacc aatcattgta ataacctctt aaaattccca attaacctag
    24301 actaatctga acgtcaacgc cagcagctag atctaaacgc attaatgcat caacagtttt
    24361 ttctgttggt tcaacaatat caactaaacg tttgtgagta cggatttcat attggtcacg
    24421 tgcatcttta ttcacgtgtg gagaaatcaa tacagtgaaa cgttctttac gagttggtaa
    24481 aggaattgga ccacgaactt gtgcaccagt acgtttagct gtttctacga tctccgcagt
    24541 agattgatca attaaacgat gatcaaatgc ttttaagcgg atacggattc tttggttctg
    24601 cattagacca gagctccaat taaaaaattt agctaataaa aaaaccaaac taccacttaa
    24661 caaattagtt aagggagctc agttaaacct atatagttcc aaaattagga acattgtata
    24721 cactacaata tctgtagtgc catttgataa atgattacat caaatgtctt tagataccta
    24781 aagctttgct atcatactcg tttcatttcc cttatgcaag taatttttgt tttttatatt
    24841 gacaataatt tgattcgata aaacttctaa agatacacaa tatttccaat acttcatttg
    24901 aagtggctca aataaattgt atttatgcat ttttttattc aaacttttcg agccacttca
    24961 caaatctaaa aaatcctagt tgaaatttaa tccataagag tatttattat taaataagtt
    25021 tcatgacctt acatcggagt acatatatgc aaacaatcaa tgaaattaaa gaagaaatct
    25081 accctatatt tgagcctatc attggaacaa ttcggctaaa aacctacttt tcctactacg
    25141 cagtttttaa ggatggattg atgattgcat tgtatcaaaa tggagcaact tatttacgta
    25201 ttgcgaaaaa agatattgaa tacattcgtc agtatcctga aacctgtatc ttatgcgatg
    25261 aaaaaattgg tatacaaagt agaaaatttc attatattcc gcaagttctc atagatgata
    25321 caccaaaatt tagctcgcta ctctttggaa ttatagagga actccgaacg gagaagtaca
    25381 aaataagcca aaagcgagct acacaaatcc gaaacctacc aaatatgaat ttaaagttag
    25441 aaagaatgtt gaaaaaaata gggattcatt ccattcatga ttttactcaa gaagggtata
    25501 tgtcaacttt tatcaaactg attctccagg gctttgatgt taccgaagac ttactgttta
    25561 agctaaacgg agctttaaat caccaatata tttacacctt cacagatcaa caaaaaaggg
    25621 aactaatgca agaagcaaat caagcgctat ataattcagg cctaagaaaa agatttaacg
    25681 ccctgtagtt aaataatgtg atccaaatca taaaatataa aaaaaccaat aaggaattgc
    25741 aattcgcagt tcctttcttt ttcttttata tgctagtatc accttatacc tctaaattat
    25801 tagtgcctac tgattttcaa ttaatccaac aatttaggag agcgaatcat ggaattccgt
    25861 atcgaaaaag acacaatggg cgaagtgcaa gttcctgccg ataaatactg ggcggcgcaa
    25921 accgagcgtt cacgcaataa cttcaaaatc gggccggagg catcaatgcc ggcggaaatt
    25981 atcgaggcgt ttggctatct gaaaaaagcg gctgcctatg ctaatcacga tttaggcgta
    26041 ctagcggtcg aaaaacggga tttaattgca aaagcctgtg atgaaatttt ggcgaacaaa
    26101 ctcgatgatc aattcccgct ggtgatttgg caaaccggct cgggtacgca gtcaaatatg
    26161 aacgtgaatg aagtcgtggc aaaccgtgca cacgtgttaa acggcggtaa gctgggtgaa
    26221 aaatcgatta ttcacccgaa tgatgatgtg aataaatccc aatcttctaa cgacacctac
    26281 ccaactgcaa tgcacatcgc tgcttacaaa aaagtggtgg aacacaccct gccttgtgtg
    26341 aaacgtctgc aaaaaacctt agcggaaaaa gccgaagcct ttaaagatgt ggtcaaaata
    26401 gggcgtactc acttaatgga tgctaccccg ctcacgctcg gtcaagagtt ctctgcctat
    26461 gcagcacagt tagcattcgg catcaaagca ttggaaaaca ccctgccaca cctgcgtgag
    26521 ctggcactcg gcggcacggc agtcggcacc ggcttgaata ccccgcaagg ttacgatgtg
    26581 aaagtggcgg aatacattgc gaaattcacc ggtttaccgt tcgttaccgc ccaaaacaaa
    26641 tttgaggcat tagcgactca cgatgcgatt gtcgaaaccc acggtgcatt aaaacagctt
    26701 gcggtgtcgc tctacaaaat tgccaacgat gtccgtttat tagcctccgg tcctcgttca
    26761 ggcattggcg aaattctgat tccggaaaac gagccgggtt catcaattat gccgggtaaa
    26821 gtaaacccaa cccaatgcga agcaatgaca atggtatgtg cccaagtatt aggcaacgat
    26881 gtaaccatct cttttgccgg cacgcaaggg catttccaac tgaatgtcta taaaccggtg
    26941 atggcgtaca acttcctgca atcggctcaa ctgctaggcg atgcgtgcgt gtcgtttgac
    27001 gaacactgtg cagtcggcat tgaaccgaac taccctcgca ttaaacaaca gctcgaccaa
    27061 tcgctgatgt tagtgaccgc attaaatact cacattggtt atgaaaatgc agcgaaaatc
    27121 gccaaaactg cccacaaaaa cggcacaaca ctgaaagaag aggcgattaa cttaggctta
    27181 gtcacggcag aacagttcga tgaatgggtt cgtccggaag atatggttgg cagcttgaaa
    27241 taagcccaac ccttctcttg taatctgatt tacacctcaa tctaccggta agattgaggt
    27301 gtaatttttt atagcgaggg tactgatttt gttttagaat gaagcatcat tttttaaacg
    27361 gaaaaaccta tgaaaaaact cattttaatc gcattagtta ctactttagg cttaagtgct
    27421 tgtggggtca aaggcccact ctacttccct gagcaagccc ctaaacatga acaaaaataa
    27481 gcattgcaag cggtctgttt tggctaaaat tttgcaaatt tttgtgaaaa aatgaccgct
    27541 tgttgcctct tttctttcaa aatcccaaac acaacggact ttcaatgaac cattttgaat
    27601 ataaaaacaa ccaacttttt gctgaagaag tcgctattgc cgatattatc gagcaatacg
    27661 gcacgccagc ttatgtttac tccagatcta cccttgaacg ccacttgcac gcattcaata
    27721 atgcgtttgg ctcgcaccca catttagttt gctttgcagt caaatccaac tctaacattg
    27781 ccttgttaaa tgtaatggct cgtctcggtt caggctttga tattgtctct caaggcgagt
    27841 tggagcgtgt cttagctgcc ggcggcgaag cctctaaagt tgtcttttcc ggtgtggcaa
    27901 aatcgcacag cgaaatcaaa cgagcattgg aagtcgggat tcgttgcttt aacattgaat
    27961 cggtttccga acttcaccgc attaaccaag ttgccggtga gctaggcaaa atcgcaccga
    28021 tttcactgcg ggtaaacccg gatgtggacg cccacactca cccatacatt tccaccggtt
    28081 taaaagaaaa caaattcggc gtgagcgtta ctcaagcccg tgaagtgtat cgcttagcca
    28141 aaaccttacc gaatgtgaaa attaccggta tggattgtca catcggttcg caattaaccg
    28201 aattgcaacc ttttttagat gcaacggatc gcttaatcat cttaatggag caactgcgtg
    28261 aagacggcat tgagctacac cacttagact taggtggcgg tttaggcgtg ccttacaacg
    28321 gcgaagagcc tccgcatccg accgaatatg ccaaagcctt gctggaaaag ctcaaaggct
    28381 atgaaaactt agaaatcatt attgaaccgg ggcgtgccat taccgcaaat gccggtattt
    28441 tagtgacgaa ggtggaatac ttgaaacaaa atgaagatcg taattttgcg attgtcgata
    28501 ccggtatgaa cgatatgatt cgcccggctc tgtatgaagc ctatatgcaa attacggaag
    28561 tggataaaac cttaaaccgt gacaaagctg tttacgatgt ggtcggtccg atttgcgaaa
    28621 cctcggattt cttaggcaaa caaagagaat tggcaatttc tgaaggggat ttaatcgcta
    28681 ttcgttctgc cggtgcttat ggggcggcga tgtcttcaac ctacaactct cgcccacaag
    28741 cggcagaaat tatggttgat ggcaaccaag cccatttaat taaagcccga gccacttttg
    28801 ctgatttatg gcgtttagaa aaattattac cttaatgagg aagaaaagat gaaattatat
    28861 ggattaaccg gtgcttgttc atttgtacca cacgttgccc tagagtgggt gaaattgcgt
    28921 gccaatcaag attatgcgtt tcaagctgtt agccgtgaat ttattaaatc ggcggaatat
    28981 ctggctttaa accctcgtgg taacgtacca ttgttggttg atggcgactt ggccctcacg
    29041 caaaaccaag ctatcgtcca ctatttagac gagctttatc cggaagctaa attattcggc
    29101 agcaaaaccg ttcgtgataa agccaaagcg gctcgttggt tagctttttt caattcagat
    29161 gtgcataaat cattcgtgcc actgttccgc ttgccaagtt atgcggaagg caatgaaacg
    29221 ctcaccaaaa ctattcgcca acaatcggct gagcaaattt tagaacaact ggcttttgcg
    29281 aatgctcatt tagaaaatca tattttcttt ggcgaagaaa tttcggttgc cgatgcgtat
    29341 ctctacatta tgctcaactg gtgtcgccta ctagggttgg atttctctca tctttcccaa
    29401 ctttctgcct ttatgcaacg agtggaggca gaccaaggcg tagataatgt gcgtgagcaa
    29461 gaaggcttaa aaggttaatt caccattgtt catttaagac ggtagatttt atgttaaaat
    29521 ctaccgtctt ttatttttag gaatcaccat gctaaatcag gtttcaaaca gcttgcttgc
    29581 tccaagtggg cttgaattat cgcacttaac tcaagtgtta gatcacttct ccggccgtca
    29641 tattgattat gccgatctct acttccaact cagccaagac gaaagctgga gcttggaaga
    29701 cagcatcatc aaagaaggcg gtttttatat cgaccgaggg ttcggtgtgc gagccatatc
    29761 aggcgaaaaa accggctttg cctacgccga tcaaatcacg ttaaaacagc ttgagcaatg
    29821 tgccgctgcc gcacgttcaa tcagcccaac aagcggtcaa ttatcggtaa aaaatttcaa
    29881 aaaaacgacc gcttgttctc gctacccggc aatcaacccg ctagagacaa tcagccgtga
    29941 acaaaaggtc gaattgctcc atttggtgga taaaaccgct cgaatggcag atccgagagt
    30001 gattcaagtg aatgcgaatc tctcagcggt atatgaagaa atgttagtgg cagcgactga
    30061 cggcacactg gctgcggata ttcgcccgtt agtgcgttta tctgtgtcgg tactggtgga
    30121 acaaaatggc aagcgtgaac gaggttcagc aggtgccggt ggtcgctttg gtttagattg
    30181 gttcttagca ccaagcgaaa ccggctttag ccgtgcggaa tcgcttacca aagaagccgt
    30241 tcgccaagcc ctcgtcaatc tcacagccat tcctgcaccg gcaggcacga tgccgattgt
    30301 tctcggtgct ggctggccgg gcattttgct ccacgaagca gtcggacacg gcttggaggg
    30361 tgattttaac cgtaaggaat cttcgctctt cagtggtaaa atcgggcaaa tggtcacctc
    30421 tgagctttgc accattgttg atgacggtac ggtgccgaat atgcgaggct caatcacggt
    30481 agatgacgaa ggcgtgcctt cgcaacgtaa tgcgttaatt gaaaacggca ttctaaaagg
    30541 ctatatgcaa gacaaactca atgcccgcct gatgggagtt gccccaaccg gcaacggcag
    30601 acgggaatct tacgctcatt tgccgatgcc acgaatgacc aacacctacc tcaccgaagg
    30661 caatcacgaa tttgaagaga tgattgaatc ggtcgaatac ggtttatatg ctcctcactt
    30721 cagtggtgga caagtggata ttacctccgg caaatttgtg ttctccaccg ccgaagccta
    30781 cttaatcgaa aaaggcaaaa tcaccaaacc tgttaccggt gcaaccctga tcggcagcgg
    30841 catagaagca atgcaacaag tttcaatggt cggcaaaaaa atggaactcg accccggcat
    30901 cggcacttgt ggcaaagaag ggcaatccgt accagtcggc gtagggcagc ctacagtgaa
    30961 gttggataaa attacggtgg ggggaagagg gtaatctctc cccgtttgca aaatataaag
    31021 aaaatatcac cgcttgtagc gataaaagga aaacaaaatg gcttgggtac aaattcgttt
    31081 aaacagtaca gataaacagg ctgaggaaat cagcgatttt ttaaccgaga ttggagcggt
    31141 gtcagtgact tttatggata gtcaagatac cccaattttt gaacctcttc cgggggaaac
    31201 ccgtctttgg ggaaataccg atgtggttgg gcttttcgat gcggaaactg atatgaatct
    31261 tatcgtagaa gcactaaaaa ccagcaaatt ggtggacgaa aactttgctc acaaaatcga
    31321 gcaaatcgaa gacaaagact gggaacggga gtggatggat aatttccacc caatgcagtt
    31381 tggcaaacgg ttatggatct gcccaagctg gcgcgaagta ccagacccga atgcagtgaa
    31441 tgttatgtta gaccctggtt tagcatttgg caccggcaca cacccaacga cctcgctttg
    31501 cttacaatgg ttagatagct tagatttaga aggcaaaacg gttatcgact tcggctgtgg
    31561 ctccggtatt ttagctatcg ctgcacttaa actcggtgca aaacaagcca tcgggattga
    31621 tatcgaccca caggcgattt tagctagtca aaacaatgct gaggcgaacg gcgtagcaga
    31681 aaaactacaa ctattcttag ccaaagatca gccaagagag ttacaagccg atgtcgttgt
    31741 tgcgaatatt ctagccggcc cactcaaaga gttagcaccg aatatcatca cgctggttaa
    31801 accaaacggc aatttaggct tatccggcat tcttgctact caggctgaaa gtgtgtgtga
    31861 ggcttatagc gaagccttta cgctagaccc tgttgtagaa aaagaagaat ggtgttgtat
    31921 tacaggccgc aaaatctaat ataagaagcc ctgttttaaa cagggcttac tttttgataa
    31981 attctactac tctttacctg tcttgggaat cgttctccct aaattaatta acacaagcgg
    32041 tagaaataac aggaaaattt acaaattaca taaatgaaaa aagccgactg aaatcagtcg
    32101 gctttctaaa taatggcagg ggcggagagg ctcgaactcc caacacccgg ttttggagac
    32161 cggtgctcta ccaattgaac tacgccccta ttgataggta ttcattttgc acttataggg
    32221 gcgaattact attcgccccc tgcattaaag attattggcg gaacggacgg gactcgaacc
    32281 cgcgaccccc tgcgtgacag gcaggtattc taaccagctg aactaccgct cctaaattaa
    32341 tagattgata atgccctact ctcacatggc gaatcaccac actaccatcg gcgttactgc
    32401 gttttacttc tgagttcgga atggagtcag gtagagctac agcaccctgg ttatcaatca
    32461 aattctttca tttagtcagt ttactaaaat atagtttata ctttctaata aactcactaa
//
LOCUS       DANKBV010000011        30643 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.011, whole genome shotgun sequence.
ACCESSION   DANKBV010000011 DANKBV010000000
VERSION     DANKBV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 30643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30643
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.011"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            254..3313
                     /locus_tag="PWY43_000419"
     CDS             254..3313
                     /locus_tag="PWY43_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL2135329.1"
                     /translation="MINTLPITETNNFIVLDKYEKIIQPTAYQTETDLERELITDLQF
                     QGYEYRPDLNNQEKLLANLREQLEKLNNVRFLDSEWKRLLEEYIDKPSENIIEKTRKI
                     HHDHIYDFVFDNGRIQNIYLLDKKEIARNKVQVINQFEQKGSHLNRYDVTILVNGLPL
                     IQIELKKRGVAIREAFNQIHRYSKESFNKENSLFKFLQIFVISNGTDTRYFANTTKRD
                     KNSFDFTMSWAKSDNGLIKDLKDFTATFLQKNTLLNVLVNYSVFDVNDTLLLMRPYQI
                     AATERILWKIKHTHFTNTWNKPESGGYIWHTTGSGKTLTSFKAARLATELDCIEKVFF
                     VVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKQDNKIIVTTIQKLNNLMKSE
                     DNLPIYQQPVVFIFDECHRSQFGEAQRNLKRKFKKFCQFGFTGTPIFPENALGAETTA
                     LVFGTQLHSYVITDAIRDEKVLKFKVDYNDVRPQFRTMEIEKSPEKLTALENKKALLH
                     PERIKEITQYILAHFKQKTHRLNATGKGFNAMFAVSSVEAAKLYYEQFKIQQQAVQNP
                     LKVATIFSFAANEEQNAIGEIADETFEPTAMNSSAKEFLSLAIDDYNEQFKTNYGVES
                     KEFQNYYRDLAMRVKNQEIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSRT
                     NRIYDSTKTFGNIVTFRDLEQDTIDAITLFGDKNTRNVVLERSFDEYMQGYEKSSGEY
                     QRGYLVVVKELQTKFPDPTNLEKEEDKKAFTALFGEYLRLENVLQNYDEFHELKNPEN
                     IENIVCEPTANYLNHTQIILPERKLQDYRSTYNDIREWQRQQRQGNNTQTSTIDWSDV
                     VFEVDLLKSQEINLDYILELIFEHNKKIKNKPALIEEIRRTIRSSIDNRAKESLVVDF
                     IHQTDLDTIPDKAGVIEAFFQFAQQEQKKEAETLIETENLNVEAAKRYITTSLKREYA
                     SENGTDLNDILPKMSPLNPQYKTKKQTVFQKIVAFVEKFKGVGGYLS"
     gene            3397..5346
                     /locus_tag="PWY43_000420"
     CDS             3397..5346
                     /locus_tag="PWY43_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2135330.1"
                     /translation="MALINLSNAYLGFGDHPLLDHTELHIEPGERVCLVGRNGAGKST
                     LMKILAGEVQLDDGKLVFEKDIVVTRLEQDPPRHIEETVFDYVAEGIAHLADLLKQYH
                     HISLQMQTDYSDELLAKLSAVQSQLEHANGWQFENRIQDTLKLLELDPDKRLCELSGG
                     WVRRAALARALVADPDVLLLDEPTNHLDIEAIAWLENLLLDFKGSIIFISHDRAFIRK
                     MATRIVDLDRGKLVSYPSNYDKYLEEKAEDLRVEALQNELFDKKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKALREERRNRREVQGTAKIQIDNTARSGKIVFEVENASHEIGGRTL
                     LKNFSTTILRGDKIALIGANGIGKTTLIKLLLGEIQPTSGSVRCGTKLEVAYFDQYRA
                     DLDTEKTVMDNVADGKQDVEVNGVKRHVLGYLQDFLFPPKRAMTPVKALSGGERNRLL
                     LAKLLLKPNNLLILDEPTNDLDVETLELLEEMLTDYQGTLIIVSHDRQFVDNTVTECY
                     FFEGEGVVNKYVGGYFDAKQQQENYFASQKQSVETPRLASEKSPVKTTESDTKHRVPT
                     EDAKKKVKLSYKEQRELDELPAKMEQLEADIEALQAEVNSAEFFAKDPSYTNAQLQKL
                     ADTEAALEQAFERWEALENIKNGNV"
     gene            5376..6131
                     /gene="tcdA"
                     /locus_tag="PWY43_000421"
     CDS             5376..6131
                     /gene="tcdA"
                     /locus_tag="PWY43_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDL2135331.1"
                     /translation="MERIDNYEQRFGGIGRLYTPEGLEKLRRSHICVIGIGGVGSWAV
                     EALARSGIGKITMIDMDDICVTNINRQIHAMSGTVAQLKTEAMKERIERINPECVVEI
                     IDDFLTQDNLADYLNRGYDYVIDAIDSLRVKAAMIAFCKRNKIKIITTGGAGGQTDPT
                     KIQITDLSKTVQDPLAARVRSLLRKEYGFSNKNKFGVECVSSTQQLIFPQMGEACEVS
                     ATMNCANGFGAVTMVTATFGFFAVSRVIDKLLK"
     gene            6178..7545
                     /locus_tag="PWY43_000422"
     CDS             6178..7545
                     /locus_tag="PWY43_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HDL2135332.1"
                     /translation="MAIHRVNPKGSMEQLSHLEMELLAKNTQGNLHQLYRNCSLAVLN
                     SGVHTDDSRALLSQYPDFEIRLLTREKGVSLELHNPPETAFVDGKMILNIQYHLFAVL
                     RDIVFVNALKNAIRPLEETSLEALTTNTVFSILRNAKAIEMNTDPNLVVCWGGHSINE
                     TEYQYCRAVGQEFGLRELNIVTGCGAGVMEAPMKGAAIGHANQRYKNGRFIGITEPSI
                     IASEPPNPIVNELVIMPDIEKRLEAFVRLGHAIVVFPGGPGTLEELLYIIGIKLNPAN
                     HAQQLPLILTAPKESADYFYAIDQFIGETLGEEARSLYEIIIDDPVLVARKVKQAMIE
                     VKSSRYESGDAYNFNWSLKIEEDFITPFIPTHENMANLNLSLAQPKEQLAANLRRAFS
                     GIVAGNIKPETSDLIEKYGVFKLHGDRKLMEHIDKLLERFVVQHRMKIPTGEAYQPCY
                     EIVKG"
     gene            7565..9112
                     /locus_tag="PWY43_000423"
     CDS             7565..9112
                     /locus_tag="PWY43_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDL2135333.1"
                     /translation="MYLKHIEISGFRGINHLSLQLRPNMVLIGENAWGKSSLIDALSL
                     IFNLEQALYQFQFKDFHIQYTDTPNQVRDIQLVFTFCEEHQHDLAVQFPAYLAQAAFY
                     IDNHRCVSLAVTGKLHGEQISTDYQFVDQSLKPIQIEQAENAIRQFIYHHPVYRLRDA
                     RLNNRVHNELLLKGSSQLEDDFQRELNALSSLLKYYFLTSQSRYLIKTSMQDTALWWE
                     KVKSLCLRLKQQPELVESAQQHISTLFLFNPRSTVIEKPIILFEDLNAQLHPRMIAIF
                     WELLGFLPSQRITTTNSIELLSQVPLREICRLVRHHDHTQAYWLERHSLGKEDLRKLT
                     FHIHHNRGLALFAKAWILVEGETEVWILQELARLLDIHLEMEGIKIVEFAQCGLRPLI
                     KYARAMGIEWYVLTDGDDSGKRYADTVKSMEEDFAHRLTMLPQKDIEHFFYKAGLRNV
                     FIQLANWRSQEKYPVSYIIKRAIQRSSKPDLAIALANEMEQRGEHSIPTLFKQLFADV
                     LALINKV"
     gene            9335..10369
                     /gene="metN"
                     /locus_tag="PWY43_000424"
     CDS             9335..10369
                     /gene="metN"
                     /locus_tag="PWY43_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDL2135334.1"
                     /translation="MIQLKNISKQFEVKGKKITALDNVNLEVPRGTIYGVIGASGAGK
                     STLIRCVNLLERPTIGQVIVDGQDLTAMSDKELIHARRNIAMIFQHFNLLSSQTVFEN
                     VALSLRLSNTPKEQVEKKVNELLDLVGLTDRKDVYPANLSGGQKQRVAIARALASDPH
                     VLLCDEATSALDPATTQSILQLLKDINKRLGLTILLITHEMEVVKRICDRVAVIDKGQ
                     LIESGSVSEIFSNPKTELAQKFIQSTFHFELPQEYTEQLSSSPTEHSKPIIKFEFTGR
                     SVDAPLLSMASKKFGIDFSILMSQIDYAGGVKFGFVIAEVSGNPDSIAEAKFYLIDNN
                     VKVEVLGYVG"
     gene            10359..11036
                     /locus_tag="PWY43_000425"
     CDS             10359..11036
                     /locus_tag="PWY43_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2135335.1"
                     /translation="MWADFMQEMTPQMWSLVWESTFETVYMGFVATALAVVIGLPIGF
                     LAFLTGKGEILENPRLHQVLDVVINIGRSIPFIILLIILLPFTRLIVGTTLGTTAAIV
                     PLSVSAIPFFARLTSNALLEIPSGLTEAAKSMGATNWQVVTKFYLPESLPILINGITL
                     TLVALIGYSAMAGAVGGGGLGNLAISYGEHRNMVYVKWIATIIIVAIVMISQKLGDHL
                     AKRYDHR"
     gene            11059..11892
                     /locus_tag="PWY43_000426"
     CDS             11059..11892
                     /locus_tag="PWY43_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL2135336.1"
                     /translation="MSFKKILGVALVSALALTACKEEKKAESTAAPAAQAPAKIKVGV
                     MSGPEHTVAERAAQIAKEKYGLEVEFVLFNDYALPNTAVSKGDLDANAFQHKPYLDKD
                     SQSKGLNNLVIVGNTFVYPLAGYSKKVKNVSELAEGAVIAVPNDPSNLARALILLEKQ
                     GLIKLKDNTNLFSTSVDIIENPKNLKIKEVDTSIAAKALDDVDLAVVNNTYAGQVGLN
                     TQDHGVFVESKDSPYVNIIVARQDNKDAANVQNFIKSYQTEEVYQEAQKHFKDGVVKG
                     W"
     gene            11975..12805
                     /locus_tag="PWY43_000427"
     CDS             11975..12805
                     /locus_tag="PWY43_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2135337.1"
                     /translation="MNFKKLLGVALVSALALTACKDEKAQAPATTAKTENKAPLKVGV
                     MTGPEAQMTEVVVKIAKEKYGLDVELVQFTEYTQPNAALHSKDLDANAFQTVPYLEQE
                     VKDRGYKLAIIGNTLVWPIAAYSKKIKNISELKDGATVAIPNNASNTARALLLLQAHG
                     LLKLKDPKNVFATENDIIENPKNIKIVQADTSLLTRMLDDVELAVINNTYAGQAGLSP
                     DKDGIIVESKDSPYVNLVVSREDNKDDPRLQTFVKSFQTEEVFQEALKLFNGGVVKGW
                     "
     gene            12857..13648
                     /gene="metQ"
                     /locus_tag="PWY43_000428"
     CDS             12857..13648
                     /gene="metQ"
                     /locus_tag="PWY43_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDL2135338.1"
                     /translation="MKIMKLAGAVAIFSLFLTACNDKAEKLKVGVISGPEHKVMEVAA
                     KIAKEKYNRDVELVVFTDYATPNAALDKGDLDLNAFQHKPYLDNQIQEKGYKLVPVGN
                     TFVYPIAAYSKKIKSLAELKDGDTIAVPNDPTNLARALILLEKQGLIKLRADAGLKAT
                     SVDIIENPRKLVIQEIEAPLLPRTLDDVAFSIINTTYAGQNGLTPTKDGIFVEDKDSP
                     YVNLIVARENNQHSEAVKDLVKAYQTEEVYNKANEEFKGAMIKGW"
     gene            complement(13692..14843)
                     /gene="metK"
                     /locus_tag="PWY43_000429"
     CDS             complement(13692..14843)
                     /gene="metK"
                     /locus_tag="PWY43_000429"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL2135339.1"
                     /translation="MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACET
                     YVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPD
                     INQGVDRADPLEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMEQQSKVRKSGKLDW
                     LRPDAKSQLTFAYENNKIIGIDAVVLSTQHAEHISQKELIEGVMEEIIKPVLPSEWLS
                     QNTKYFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQLSYAIGVAAPTSIMVETFGTGKVSNEILVKLIYQNF
                     DLRPYGLIKMLDLIKPIYRETAAYGHFGRKHFPWEKTDKAEALRAGAGL"
     gene            complement(15026..15868)
                     /locus_tag="PWY43_000430"
     CDS             complement(15026..15868)
                     /locus_tag="PWY43_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDL2135340.1"
                     /translation="MKVGLVLEGGGLRGIFTAGVLDVFLDEHIHIDGAIGVSAGALFG
                     VNLPSKQKGRALRYNKKYAKDKRYMGLHSLLTTGNIVNRDFAYYELPSKLEPFDQQAF
                     EDSGMDFWVTVTNVETGEPEYIKIDNMFEQMEAFRATSSMPIVSKIVEIEGKKYLDGG
                     VSDSIPVQKFIEMGYDKIIVVLTRPLEYRKKPSSMWLFKRFYKKYPKLVQRWENRYAE
                     YNQAVEQIIQLNEKQQIFVIRPSRTVKISRLETDVNKIQAMYDLGVEDAKNALAGLRA
                     YLAK"
     gene            15928..16245
                     /locus_tag="PWY43_000431"
     CDS             15928..16245
                     /locus_tag="PWY43_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL2135341.1"
                     /translation="MADAEFMDFLLDQLSPISGLRSKKMFGEYCLFFDNKIVAIISKD
                     YRIFVKANPQTLPLFLAENAEQFHYFAKGKVQKMHYWSIPELAIEEADELVKWIRLGL
                     QAV"
     gene            16335..17048
                     /locus_tag="PWY43_000432"
     CDS             16335..17048
                     /locus_tag="PWY43_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HDL2135342.1"
                     /translation="MNQKKLVSLIVVIVLAIISQIFSFFDKKEPSKAQEKSTQSSQSH
                     QVSTSSLGNYDVQMKNDRLKQHIVDTDYYTLALSWSPAFCQKQKRNNHGKIPSHLEYQ
                     CGGSADFGWVIHGLWPQSGKARSVDEHPRYCQGDLPMVAEQTIKQYLPESPGATLLQG
                     EWEKHGACAFNQADDYFAKQKELFNQLKLPDTEMSKLALFNWMRANNPHLQNVYLGGS
                     KDELYICYDKAFQPMDCPR"
     gene            17048..17542
                     /locus_tag="PWY43_000433"
     CDS             17048..17542
                     /locus_tag="PWY43_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDL2135343.1"
                     /translation="MADLRQLKMQVQRQLKRDLERANHYFNTTFTPPSISYAVRGVKA
                     GVAYLERNEVRFNPVLLQENEQAFIRQVVPHELAHILVYQHFGRVQSHGKEWKMMMET
                     VLGVPAEIYHCFDTQSVQQQFSYQCACQTHQLSIRRHNAVMRDKKSYICRLCKTQLHF
                     VEKS"
     gene            17647..18927
                     /locus_tag="PWY43_000434"
     CDS             17647..18927
                     /locus_tag="PWY43_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2135344.1"
                     /translation="METIVIVGGGAGGLELATYLGNKLGRKQKAKVVLIDRNATHLWK
                     PLLHEVATGTIDEGTDALSYRAHAKNHSFEFQQGTLVAVDREKKTVSLAPIYNDQNQL
                     LVKERHISYDKLVIAIGSKSNDFGTKGVAENCIFLDGSEQAKDFQKRMMELFLRFSHS
                     ENKDVKIAIVGGGATGIELSAELYHVVKNLNSYGFGKLNRASLKVTLIEAGPRLIPAL
                     PEKVSVSAFHELKKAGVSVRLGTMITEARPDGLMTRLGEKIEADIMVWAAGVKAPEIT
                     REFGFETNRLNQIEIKDTLQTTVDDSVFVIGDCASLIQDGKPIPPRAQAAHQMATRCG
                     KNIVALIDGKEMKPFRFKDKGSLLSFSKFGTVGNLMGNLVSGDMFIEGKIARLAYLSL
                     YRMHQVALHGCVKTGLIVLVGQINRFLRPTMKLH"
     gene            complement(19014..20051)
                     /locus_tag="PWY43_000435"
     CDS             complement(19014..20051)
                     /locus_tag="PWY43_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2135345.1"
                     /translation="MNVLKLISLKSILIASGIFLSAQAAQAEGRLTVYCSVQNNVCEE
                     MTKQFSHKYNVETKFIHGGTGTIFGKVKAEASNPQADIWYGGTIEPHFQAGELGLLEA
                     YRSPKQAEILPQFKSLVESEQGKFTSIVYMLVLGLGVNTEKLAKLGIEAPKKWEDLSD
                     PRLKGEVQLPDPHSSGTTYTFMTTLSTLWGEEKTFDYLKKLNNNVSQYVKSTLVTSNL
                     ARGESAVTVGFVHSYATEKEKGAPIEAIIPEGKVGYALGGVSIIKNARNLDNAKLFMD
                     WVLSKEAQEIPWKKHGVYQTPTNINAEVAPQSTKADALDFVEIDYQKAGSSEEGKRLI
                     DKWLAEIKLAN"
     gene            20273..20969
                     /locus_tag="PWY43_000436"
                     /pseudo
     CDS             20273..20969
                     /locus_tag="PWY43_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249137.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            20980..21282
                     /locus_tag="PWY43_000437"
     CDS             20980..21282
                     /locus_tag="PWY43_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2135346.1"
                     /translation="MFAALVLGSQICRFLPEFLPKKVLSSPILQKLNKMLPLVIMLLL
                     LLTSLNFPKNGEGFNLILAQILALIGVLGSYVWLKNTVLSVALGIFGVNVFLSLLG"
     gene            complement(21403..22704)
                     /locus_tag="PWY43_000438"
     CDS             complement(21403..22704)
                     /locus_tag="PWY43_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2135347.1"
                     /translation="MSLFKFEERGSNARQEIIAGLTTFLAMVYSVIVVPGMLSQAGFP
                     AESVFIATCLVSGLGSILIGLWANAPMAIGCAISLTAFTAFSLVLGQQVSIPVALGAI
                     FLMGVVFTLISATGIRTWILRNLPSSIAHGAGIGIGLFLLIIAATNVKLVVNSGMDIP
                     VKLGDFTSFPVLVSLIGLAAIIGLEKLKVKGSILWVIIAITIVSLIFDPNVKYTGFFK
                     MPSFGENSQFLNLDIMGALNTAILPVVFALVMTAIFDATGTIRAVAGQAGLLDKDGQI
                     INGDKALTSDSFGSILSGLFGTAPAAVYIESAAGTAVGGKTGLTAVVVGIGFLFMLFF
                     QPLAFLVPSYATAPALMYVGLLMLSNVSKLDFSDFVGAMSGLVCAVFIVLTANIVTGI
                     MLGFATLVIGRLISGEANKLNLGTVIIAIILVAFYAFDLAI"
     gene            complement(22837..23811)
                     /gene="secF"
                     /locus_tag="PWY43_000439"
     CDS             complement(22837..23811)
                     /gene="secF"
                     /locus_tag="PWY43_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDL2135348.1"
                     /translation="MAIENVQQDVAEIKLPYKLIPFMKYRYVGFIFSLIVTALCIFTI
                     ATKGFNWGLDFTGGTVIETQFSQPADLAKLRTELENSGYGNALVQTTGSQKDLMIRLA
                     ASAGNMSVGNKVMEMIHQKLDPNATIKSIEFVGPNVGEELTQGAIYATLATLAMLLIY
                     VGTRFEWRLAVGGIVALFHDVLVTIGIFSFLQIEIDLTFVAAILSVVGYSLNDSIVVF
                     DRVRENFSKVRRSSSIEIIDISLSQTLSRTLMTSVTTLFVVLALFYLGGPTLHSFSLA
                     LLIGIGFGTYSSIYIAIGVALQLGLKREHMVQPKVEKEGADQDAFIDY"
     gene            complement(23821..25662)
                     /gene="secD"
                     /locus_tag="PWY43_000440"
     CDS             complement(23821..25662)
                     /gene="secD"
                     /locus_tag="PWY43_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDL2135349.1"
                     /translation="MLNRFPLWKNLMVIFVIAIGALYALPNLYGDDPSVQISGTRGQQ
                     ATPETLSQVQSTLSAMNIQPKSAVLENGSILVRLESSEQQLPAKEKISEALGNNYSVA
                     LNLAPATPQWLSDIGGSPMKRGLDLLGGVRFLMEVDMNTALAKQQDTLQDSLRSDFRK
                     EKLQYQAVKKGENFATEVEFADNDTAEKAVRLVRLSHPTLDILVNDNKVTLTPSAQAL
                     SESRTMAIEQNLSILRKRVEELGVSEPTIQRQGADRIVVELPGVQDTARAKELLGATA
                     TLEFRLVNTEANVASAAKGIVPADSELQNTRDGEPVVLRRKPSLGGEHIIDATSGTDE
                     RGLPQVSIRLDTEGGEMMGEITKMAIQKPMATLYTEFKDSGRKDANGKVILEKHQEVI
                     NVATIQTRLGNQFQITGINSPAEAQNLAVLLRSGALIAPIVIVEERTIGASLGADNVK
                     QGMEASILGLGLTILFCLAFYKVFGIFASLALIVNMILTIGLMSLIGATLTMPGIAGI
                     VLAVGMSVDANVLIYERIKEEIRNGRPIQQAIHEGYNGAFSSIFDSNLTTILTSLVLY
                     GVGTGPVKGFAVTLALGVIISMFTAITGTRMLVNWVYGGKRVKKLWI"
     gene            complement(25702..25995)
                     /gene="yajC"
                     /locus_tag="PWY43_000441"
     CDS             complement(25702..25995)
                     /gene="yajC"
                     /locus_tag="PWY43_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL2135350.1"
                     /translation="MQQGGGMEMIFILIIFGAIFYFMIYRPQAKRQKQQRDLLASLTK
                     GDEVLTSGGLIGKITKVTADNDNIVIALNDTTEVTIKRDFVVAVLPKGSLKSL"
     gene            26196..26753
                     /locus_tag="PWY43_000442"
     CDS             26196..26753
                     /locus_tag="PWY43_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDL2135351.1"
                     /translation="MKKLFAQAVIFGAFFASSAFAHNVQLNQALPAVSVAKDGELAVA
                     GGKVSYKNWHSSSLAGKVRVIHHFAGRSSVKEKNEDLMTAIKNAGFDRSKYQTVTIVN
                     ADDAIVGTGVFVKNSVENGKLDNPHSQVVLDQQGSVKKAWGLKEKESFIAVLDKSGKV
                     QFTSEGKLSAKQIQEVIGLVKTLTQ"
     gene            complement(26794..27762)
                     /locus_tag="PWY43_000443"
     CDS             complement(26794..27762)
                     /locus_tag="PWY43_000443"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDL2135352.1"
                     /translation="MKLFTLENAFLRITLSDVGASWLSCIVKMPNEEREVLVTTSAKN
                     WQKQTAFFGATIGRYANRIANAEYRLNGKTYQLAKNNGEHNLHGGELGADKIVWQLES
                     QSEQAVKFSYFFADGEEGFGGEVKAFVEYRLTENELQISFDAVANQDTPLCLTNHAYF
                     NLQGSETILDHELIINADGYLPVDASGIPNAPLKKVESTSFDFRNAKKIGQDLLADDD
                     QKAVKGYDHSFHLAKNSENPTACLAVEDLKMELRTSMPALQLYTGNWLKGQPNLTKGE
                     YEDYAGVALEPEFFPNSLNQPELLQFGGITKAGEEYHHTISYRFII"
     gene            complement(27888..29051)
                     /gene="galK"
                     /locus_tag="PWY43_000444"
     CDS             complement(27888..29051)
                     /gene="galK"
                     /locus_tag="PWY43_000444"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL2135353.1"
                     /translation="MKPQEIALQQFEQHFGYKAERTVFAPGRVNIIGEHTDYNDGFVM
                     PCAINYGTAVSFAKRSDNKWRVFAIDLNQQDEFDLSQAIEPSEQKWANYVRGVVKFIQ
                     AKHPEFKQGADLAITSDVPMSSGLSSSAALEVSIGKTAQLLGDLPLSLPQIALIGQEA
                     ENKFVGCNCGNMDQLTSALGQQDHLVMIDCRSLEITPTPVPQGYSIAIINSNVKHDLV
                     TGEYNSRREQCEFAAHFFGKAKLRDVTAEQFIERASELSEENELAYKRAKHIISENQR
                     VLEAVEALKANDMVKLGELMAQSHDSMRDDFEITCPEVDCLAEIAQQAIGKNGGARMT
                     GGGFGGCVVCLVPNDKVEDLRQAVANNYEKQTGIKETFHVCTASDGVRECPAR"
     gene            complement(29061..30107)
                     /gene="galT"
                     /locus_tag="PWY43_000445"
     CDS             complement(29061..30107)
                     /gene="galT"
                     /locus_tag="PWY43_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDL2135354.1"
                     /translation="MSEFVLNDHPHRRFNPLKNQWILVSPHRAKRPWQGQQEEIVQDE
                     KPSYDPSCYLCPGNKRITGEQNPNYTKPFVFKNDFSALLPDTPAPIPNDDPLFQISHT
                     QGESRVICFSPDHSKTLPQLSIEEITDVVTVWQEQANELKEHYQWVQIFENKGSMMGC
                     SNPHPHGQIWASNFLPNEIAIEDECQAKYFAQYGSPLLLDYAQRELDKQERIVAETED
                     WIAVVPYWAAWPFETLLLPKAKQFKSITDLNEAEKADLALALKKLTTRYDNLFNISFP
                     YSMGFHFAPFNGKENAHWQLHAHFYPPLLRSATVRKFMVGYEMMAESQRDLTPEQAAE
                     RLNAVSDTKHYKDK"
     gene            30320..>30643
                     /locus_tag="PWY43_000446"
     CDS             30320..>30643
                     /locus_tag="PWY43_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 family protein"
                     /protein_id="HDL2135355.1"
                     /translation="MERLNKFDAWLMNHKPNARLSGASRLLTEFWFFGLKEARACLFA
                     GLFFIAMFSVPKTGLWGLPRYDILLIFAIVVQIAMVKLGLETWDELKAISLFHLVGFV
                     LEVFKT"
BASE COUNT         9192 a         5902 c         6696 g         8853 t
ORIGIN      
        1 tacctaaaag taatgaaaat aatccagagt tatataataa gaggattgaa tcatatacaa
       61 atagaagagt taattttgga agtcattcaa agcattctgg agaagaaatg ccatttgtaa
      121 attctaatga caaaagagtt ttaaaatttc ttgttactta tttaagtgaa agtaaagatg
      181 taaataaaat tttcaaattt aaaccgagat atattaagtt tgcattttta aatataaatg
      241 agggctaatg attatgataa atactctccc tatcaccgaa actaacaatt ttatcgtatt
      301 agataaatat gaaaaaatca ttcagccaac cgcttatcaa actgaaacgg atttagagcg
      361 tgaattaatt actgatttac aatttcaggg ctatgaatat cgtcctgatt taaataacca
      421 agaaaaatta ttggcaaatt tgcgtgaaca gttggaaaaa ttaaataatg tgcggttttt
      481 agattcagag tggaaacgat tattagaaga atatattgat aaaccgtctg aaaatattat
      541 tgagaaaacc agaaagatcc atcacgacca tatttatgat tttgtgtttg ataatgggcg
      601 gattcaaaat atctatttgt tggataaaaa agaaattgct cgtaataaag tacaggttat
      661 caatcaattt gagcaaaaag gttctcatct taatcgctat gatgtaacga ttttggtcaa
      721 tggcttgcca ttaatacaaa ttgaattgaa aaagcgaggc gtggcaattc gggaagcttt
      781 taatcaaatt catcgttata gtaaagagag ttttaataaa gaaaattctc tgtttaagtt
      841 tttgcagatt tttgtcattt ctaacggcac agatacccgt tattttgcca atacgaccaa
      901 gcgggataaa aacagctttg attttacaat gagttgggca aaatcggata atggcttaat
      961 taaagatcta aaagatttta ccgctacctt tttacagaaa aataccttat taaatgtgct
     1021 agtgaattat tcggtatttg atgtaaatga cacgttatta ttgatgcgac cttatcaaat
     1081 tgcggcaacc gagcgtattt tatggaaaat aaaacatacg cattttacca atacttggaa
     1141 taaaccggag agtggtggtt atatttggca tactaccggt tcgggtaaaa cgctgaccag
     1201 ttttaaagcg gctcgattgg caaccgaatt agattgtatt gagaaagtct tttttgtggt
     1261 ggacagaaaa gatctcgatt atcaaacaat gaaagagtat caacgttttt caccggatag
     1321 cgtaaacggt tcggaaagta cagccggttt gaagcgtaat cttgaaaagc aagataataa
     1381 aattattgtt accactattc aaaaattaaa taatttaatg aagagtgaag ataatttacc
     1441 aatatatcaa cagccggtag tgtttatttt tgatgagtgt catcgtagcc aatttggtga
     1501 agctcaacgt aatttaaaac gaaaatttaa aaaattttgt caattcggtt ttaccggcac
     1561 ccctattttc cctgaaaatg cattaggggc agaaacaacc gcattggtat ttggtacaca
     1621 gctacactct tatgtgatta ccgatgcgat tcgtgatgaa aaagtgctga aatttaaggt
     1681 cgattataat gatgttcgcc cacaatttag gacaatggaa attgaaaaaa gtcctgaaaa
     1741 actcaccgca cttgagaata aaaaagcctt attgcaccct gagcggatta aagaaattac
     1801 ccaatatatt ttggcacatt ttaaacaaaa aacgcaccgt ttgaatgcca caggcaaagg
     1861 ctttaatgca atgtttgccg tcagttctgt ggaagcggca aaactctatt atgagcagtt
     1921 taaaattcag cagcaagcgg tgcaaaatcc gttaaaagtt gcaacgattt tttcttttgc
     1981 tgccaatgaa gagcaaaatg cgattggtga aattgccgat gaaacctttg aacctaccgc
     2041 aatgaatagt tcggcaaaag aatttttgag tttggcgatt gatgattata acgagcaatt
     2101 taaaacaaat tatggtgtag aaagtaaaga atttcaaaat tattaccgtg atttagcaat
     2161 gcgggtgaaa aatcaggaaa ttgatttatt gattgtagta ggaatgtttt taacaggctt
     2221 tgatgctcca acgttaaata ccttatttgt ggataaaaat ctgcgttatc acgggttgat
     2281 gcaagccttt tcccgcacta accgcattta tgactctact aaaactttcg gcaatatcgt
     2341 gactttccga gatttggaac aggatacgat tgatgcgatt acgctatttg gcgataaaaa
     2401 tacccgtaat gtggtgttag aacgtagttt cgatgaatat atgcagggct atgaaaagag
     2461 cagtggtgaa tatcaacgtg gttatttggt ggtagttaaa gaattacaaa ctaaatttcc
     2521 tgatccaacg aatcttgaaa aagaggagga taagaaagct tttactgcat tatttggtga
     2581 atatttacgt ttggaaaatg tattacagaa ttatgatgag tttcacgaac tgaaaaatcc
     2641 tgaaaatata gagaatatag tttgtgagcc gactgcaaat tatcttaatc acactcagat
     2701 tattttgcct gaacgaaaat tgcaagatta tcgttcaacc tataacgata ttcgggaatg
     2761 gcaacgccaa caacgacaag ggaataatac tcaaacttca acaattgatt ggagtgatgt
     2821 ggtgtttgaa gtggatttac taaaatccca agagatcaat ttagattata ttttagagct
     2881 tatttttgaa cacaataaga aaatcaaaaa taagcctgcg ttaattgaag aaattcgccg
     2941 tactattcgt tcaagcattg ataatcgagc gaaagaaagt ttggtagtgg attttattca
     3001 ccaaaccgat ttggatacta ttcctgataa agccggtgtc attgaggcat tcttccaatt
     3061 cgcccagcaa gaacagaaaa aagaggcaga aaccttaatt gaaaccgaaa atttaaatgt
     3121 agaggctgca aaacgttata tcaccacttc actaaaaagg gaatatgcca gcgaaaacgg
     3181 cacggattta aatgacattc tgccgaaaat gagcccactt aatccgcaat ataaaactaa
     3241 aaaacagacg gttttccaaa aaatcgtggc gtttgtggag aagtttaagg gagttggtgg
     3301 gtatttatct taacaagaat aaaaacgagt agaacagaac gctttcatac aagcggttag
     3361 agaattgcta aaatttacaa ttttaacgag aatactatgg cacttatcaa tctttccaac
     3421 gcctatttag gctttggcga tcatccttta ttggatcata ctgaattaca tatcgaaccc
     3481 ggcgagcggg tttgtttggt ggggcgtaac ggggcgggta aatcgaccct gatgaaaatt
     3541 ctggcgggcg aagttcagct tgatgacggc aagttggtat ttgaaaaaga cattgtcgtt
     3601 acacgccttg aacaagatcc gccccgccat attgaagaaa cggtgttcga ttatgtggca
     3661 gaaggcattg cccatcttgc cgacttattg aagcaatatc accacatttc cctgcaaatg
     3721 cagaccgatt acagcgatga actgctcgcc aaactttcag cggtgcagtc gcaattagaa
     3781 cacgctaacg gttggcagtt tgaaaaccgc attcaagaca ccttgaaact gttggaactc
     3841 gaccccgaca aacgcctgtg cgaactctcg ggtggttggg tacgccgtgc ggcattggct
     3901 cgggcgttgg ttgccgatcc tgatgtattg ctgttggacg agccgaccaa ccacctcgac
     3961 atcgaggcga ttgcgtggct ggaaaatctg ttgttggatt tcaaaggcag cattattttt
     4021 atctcgcacg accgtgcctt tatccgcaaa atggcgactc ggattgtgga cttggatcga
     4081 ggcaaactgg tttcctaccc aagcaattac gataaatact tggaagaaaa agccgaagat
     4141 ctgcgagtgg aagcgttgca aaatgagctg ttcgacaaaa aactcgccca agaagaggtc
     4201 tggatccgtc aagggattaa ggcaagacgc acccgaaacg aagggcgtgt gcgggcgttg
     4261 aaagcgttgc gtgaagagcg tagaaaccgc cgagaggtgc aaggcacggc gaaaattcag
     4321 atcgacaata ctgcacgcag cggcaaaatt gtgtttgaag tggaaaatgc cagccatgaa
     4381 atcggcggca gaaccctgct gaaaaatttc agcaccacca ttttgcgtgg cgataaaatt
     4441 gccttgattg gggcgaatgg gatcggcaaa accacgctga ttaagctgtt acttggcgaa
     4501 attcagccga catcaggttc ggtgcgttgt ggcacaaaat tggaagtggc gtactttgac
     4561 caataccgag ccgatcttga cactgaaaaa accgtgatgg acaacgtggc agatggcaag
     4621 caagatgtag aagtaaacgg ggtgaaacgc cacgttcttg gctatctgca agacttcttg
     4681 ttcccgccca aacgggcaat gacaccggtg aaagcccttt cgggtggtga acgcaaccgc
     4741 ttgttgctgg caaaactgtt actcaaaccg aacaacttgc tgattttgga cgaaccgacc
     4801 aacgatttgg acgtggaaac cctcgaactg ctggaagaga tgctcaccga ctatcaaggc
     4861 accttgatta ttgtcagcca cgaccgtcaa tttgtggata ataccgtaac cgaatgctac
     4921 ttcttcgagg gcgaaggcgt ggtgaataaa tatgtcggcg gctatttcga tgccaaacaa
     4981 cagcaggaaa attactttgc ctcccaaaag cagagcgtag agacgccacg cttggcgtcc
     5041 gaaaaatcgc ctgtaaagac tactgaatcg gacacgaagc atcgtgtccc tacggaagat
     5101 gcgaagaaaa aagttaaact ttcctacaaa gaacagcgtg aactggacga gttaccggct
     5161 aaaatggaac agctcgaagc cgatattgaa gccttgcaag ccgaagtaaa cagtgccgaa
     5221 ttcttcgcca aagatccaag ctacaccaat gcccagctac aaaaacttgc cgacaccgaa
     5281 gccgcgttag aacaagcctt tgaacggtgg gaagcgttgg aaaatattaa aaatgggaat
     5341 gtgtaagcgg tcatttttac ggaaaatttt acaaaatgga acgaattgat aactacgaac
     5401 agcgttttgg tggcattggt cgcctttata cgccggaagg attggagaaa ctacgccgtt
     5461 cgcatatttg cgtgattggg attggcgggg ttggctcgtg ggcagtggag gcattggctc
     5521 gctcaggtat cggtaagatc acgatgatcg atatggacga tatttgcgtg accaatatca
     5581 accgccaaat tcacgcaatg agtggcacgg tggctcaact gaaaaccgaa gcgatgaaag
     5641 agcggattga gcggatcaac cctgaatgtg tggtggaaat tattgatgat ttcttaacgc
     5701 aggataatct cgcagactac ctcaatcgag gatatgacta tgtgatcgat gcgattgaca
     5761 gtttgcgggt aaaagcggcg atgattgcgt tttgtaaacg caataagatc aaaattatca
     5821 ccaccggcgg ggcgggcggg cagaccgatc cgaccaaaat tcaaattacc gatttaagca
     5881 aaacggtgca agatccgttg gcggcaaggg ttcgtagcct gttgcgtaaa gagtacggtt
     5941 tcagcaataa aaacaaattc ggcgtggagt gcgtttcttc gactcagcaa ctgattttcc
     6001 cacaaatggg tgaggcctgt gaggtgtcgg caacaatgaa ctgtgccaac ggctttggag
     6061 cggtgacaat ggtgacggca acgttcggct tttttgcggt gagtcgggtg attgataaac
     6121 tattaaaata acaagcggtc atattaaatg aattttttgc aaatcaagga gtaaataatg
     6181 gcaatacatc gagtcaaccc gaaagggagt atggaacagc tttcccattt ggaaatggag
     6241 ttgttggcaa agaatacgca gggtaatttg catcaacttt atcgtaattg ctctttggcg
     6301 gtgctaaatt ccggtgtgca taccgatgac agccgagcgt tgttaagcca ataccctgat
     6361 tttgaaatcc gcttattaac ccgtgaaaaa ggcgtgtcgc ttgaattgca taatccaccc
     6421 gaaactgcgt ttgttgacgg caagatgatt ttgaatattc aatatcacct ttttgcggtg
     6481 ctgcgggata ttgtgtttgt gaatgcgtta aaaaatgcga ttagaccgct tgaagagact
     6541 tctttagaag ccttaaccac gaataccgta ttttcgattt tgcggaatgc gaaagcgatt
     6601 gaaatgaata ccgacccaaa tttggtggtg tgctggggag ggcattcgat taatgaaacc
     6661 gaataccaat attgccgtgc cgtcgggcaa gagtttggct tgcgtgagct gaatattgtg
     6721 accggttgcg gagccggtgt gatggaagcc ccgatgaaag gagcggctat cggacacgcc
     6781 aatcaacgtt ataaaaacgg gcgttttatc ggcattaccg agccgtctat tattgcctcc
     6841 gagccgccaa acccgattgt gaatgaattg gtaattatgc cggatattga aaaacggttg
     6901 gaagcctttg tgcgtttagg gcacgccatt gtggttttcc cgggcgggcc gggaacgctg
     6961 gaagagttgc tgtatattat cggtattaag ttaaacccgg ctaaccatgc tcagcaatta
     7021 ccactgattt taaccgcccc gaaagaatcg gcagactatt tctatgccat cgatcaattt
     7081 attggcgaaa ctttaggcga agaagctcgc agtttgtatg aaattattat tgatgacccg
     7141 gtgttggtgg ctcgtaaagt caagcaagcg atgattgagg taaaaagcag ccgttatgaa
     7201 agtggtgatg cttataattt caactggtcg ttaaaaattg aggaagattt tattacgcca
     7261 tttattccaa cccacgaaaa tatggcaaac ctgaatttaa gtttagcaca gccaaaagag
     7321 cagctggctg cgaatttaag acgggcattt tcagggattg ttgcaggtaa tattaagccg
     7381 gagacctccg atttaattga gaaatatggg gtgtttaaat tacacggcga tcgtaaatta
     7441 atggagcata tagataaact gttggagcgt tttgtggttc aacaccgtat gaaaattcca
     7501 acgggtgagg cgtaccaacc ttgctatgaa attgtaaaag ggtaatgcta acaaggggta
     7561 aggtatgtat ttaaagcata ttgaaatcag tggctttcgt ggtattaacc atttatcgct
     7621 ccagctacga ccaaatatgg tattgattgg cgaaaatgcg tgggggaaat ccagtctgat
     7681 tgatgcatta agcctgattt ttaaccttga gcaggcgttg tatcaatttc agttcaaaga
     7741 tttccatatt caatacaccg atacccccaa ccaagtgcgg gacattcaac tggtgtttac
     7801 cttttgtgaa gagcatcagc acgatttagc ggtgcaattt cccgcttatt tggcacaagc
     7861 cgcgttttat attgataatc accgctgtgt ttcgcttgcc gtaaccggaa aattacacgg
     7921 agagcagatt agcaccgact atcaatttgt ggatcaaagc ctaaaaccga tccagataga
     7981 acaagcggaa aacgccattc ggcagtttat ttaccatcac cctgtttatc gcctgcgtga
     8041 tgcaaggctc aataacaggg tgcataatga attattactt aaaggatcgt ctcaacttga
     8101 agacgatttt caacgtgaat taaacgccct ctcttcgcta ctgaaatact attttctaac
     8161 cagccaaagt cgctatttga tcaaaaccag tatgcaagac accgcattgt ggtgggaaaa
     8221 agtaaaatcc ctctgtttac gcctaaaaca gcagccggaa ttggtcgaat ctgctcagca
     8281 gcatatttct actctatttt tgtttaatcc tagatctaca gtaatagaaa agccgattat
     8341 tttatttgaa gatctgaatg ctcaactaca ccctcgaatg attgcgattt tttgggaact
     8401 gctcggtttt ttacccagcc aacggatcac cactactaat tctattgaat tgttatcgca
     8461 agtgcctctg cgtgaaatct gccgtttggt gcgtcatcac gatcataccc aagcctattg
     8521 gcttgaacgc cacagtttag gcaaggaaga tttgcgaaaa ttaacctttc atattcacca
     8581 taatcgtggc ttggcactgt ttgctaaagc gtggattttg gttgaggggg aaacggaggt
     8641 ttggattttg caagagctgg cgagattatt agatattcat ttggaaatgg aagggatcaa
     8701 aatcgttgaa tttgctcagt gtggcttacg tccgttaatt aaatacgcca gagcaatggg
     8761 gatcgaatgg tatgtgctga ctgacggtga cgattccggc aaacgttatg cagatacggt
     8821 aaaaagtatg gaagaggatt ttgctcaccg gctcacgatg ttgccacaaa aagatatcga
     8881 acattttttc tacaaagcag gcttgcggaa tgtgtttata cagcttgcaa attggcgttc
     8941 acaagaaaaa tatccggtgt cctacatcat taaacgagcg attcaacgaa gctcaaagcc
     9001 tgatttagcc attgcattag ccaatgagat ggagcaacgg ggagagcatt caattccaac
     9061 tttatttaaa caattatttg cagatgtgct ggcattgata aacaaagtat agaatacaag
     9121 cggttaaatt tgacaaaaaa ttgccaattt tgaccgcttg tttgcttgtg atgaaaacca
     9181 atcatacaaa actctaattt atttcaagtg caagctatta ttttatgcgt tatgcttatt
     9241 tggaataaaa aaatcgggtt agttgattga catagcattc tagacgtcta aaataaccaa
     9301 aaataatagt taattgaatg tggataaata gaaaatgatt cagctgaaaa atatcagcaa
     9361 acaatttgag gtaaagggca aaaaaattac cgctctggat aatgtaaacc tagaagtacc
     9421 aagaggcact atttatggtg tcattggggc atcaggggca ggaaaaagta ccttaattcg
     9481 ctgtgtaaat ttattagaac gcccaacaat cgggcaagtg attgtcgatg ggcaagattt
     9541 aacggcaatg tcggataaag agttgattca cgctcgccgt aatattgcaa tgattttcca
     9601 acattttaat ttgctttcat cacaaactgt atttgaaaac gtggcgttat cgttgcgttt
     9661 aagcaatacg ccaaaagagc aagttgagaa gaaagtcaat gagctgttgg atttagtggg
     9721 gttaacagat cgtaaagatg tttaccctgc aaatctttcc ggtgggcaga aacagcgtgt
     9781 agcgattgct cgtgcgttag ccagcgatcc acacgttttg ttgtgtgatg aagcgaccag
     9841 tgcgttagat ccggcgacta cgcaatccat tttgcagcta ttaaaagata ttaataaacg
     9901 tcttggtttg acgattttat taatcactca cgaaatggaa gtggtaaaac gcatttgtga
     9961 ccgagtagca gtgattgata aagggcagtt gattgaatct ggctcggtaa gtgaaatttt
    10021 ctcgaatccg aaaaccgaat tagctcagaa atttattcaa tcgactttcc attttgaatt
    10081 accgcaagaa tataccgagc agctttcaag ttcgccgact gaacatagta agccgattat
    10141 taaatttgag tttaccggac gttcggtgga tgcacctttg ctttcaatgg catcgaagaa
    10201 attcggcatt gattttagta ttttgatgtc gcaaattgat tatgcaggcg gggtcaaatt
    10261 tggatttgta attgcagaag tgagtggtaa tccggattcc attgcagaag caaaatttta
    10321 tttaattgat aacaatgtaa aagtagaggt gttaggctat gtgggctgat tttatgcagg
    10381 agatgacgcc acagatgtgg agcttagtat gggaatctac ttttgaaacc gtttatatgg
    10441 gctttgtggc aacggcattg gcggtagtca tcggtttacc gattggtttc ttggcgtttt
    10501 taaccggtaa aggcgaaatt ttagaaaatc cgagattaca ccaagtgtta gatgtcgtta
    10561 ttaacatcgg gcgttcgatt ccgttcatta ttttattgat tattttgttg ccatttactc
    10621 gcttaattgt gggcacaaca ttaggcacaa cggcagcgat tgttccttta agtgtttcgg
    10681 ccattccgtt ttttgcccgt ttaacttcaa atgcgttgct tgaaattccg tccggtttaa
    10741 cagaagcggc aaaatcaatg ggagcaacaa attggcaggt agtgacaaaa ttttatttac
    10801 cggagtcatt accgattttg attaatggca ttacgctaac gctggttgct ttaattggct
    10861 actcagcaat ggcgggggct gttggtggcg gcggtttagg caatttagcg attagctatg
    10921 gcgaacaccg taatatggtg tatgtgaaat ggattgctac cattattatt gttgcgattg
    10981 tgatgattag ccaaaaattg ggtgatcatt tagcaaaacg ttacgatcat cgttaatttt
    11041 atttaaaaag aggtttctat gagtttcaag aaaattttag gcgttgcatt ggtttctgca
    11101 ttagcattaa ccgcttgtaa agaagagaaa aaggcagaat ctaccgctgc tccggcagct
    11161 caagctccgg ctaaaatcaa agtaggggta atgtcaggcc ctgagcatac cgttgcagaa
    11221 cgtgcagcac agattgcaaa agaaaaatat ggtttagaag ttgagttcgt tttatttaac
    11281 gactacgctt taccgaatac tgcagtaagt aaaggtgatt tagatgcaaa cgcattccaa
    11341 cacaaaccat atttagataa agacagccaa tcaaaaggtt taaacaactt agtgattgtg
    11401 ggtaacacct ttgtttatcc gttagcgggc tactctaaaa aagtgaaaaa tgtgtctgag
    11461 ttagcagaag gtgcggtaat tgcagtacca aatgatcctt caaacttagc tcgtgctttg
    11521 attttattag aaaaacaggg gttaattaaa ttaaaagata ataccaattt attctcaact
    11581 tcagtggata ttatcgaaaa tccgaaaaac ttaaaaatta aagaagtgga tacttcaatt
    11641 gccgcaaaag cgttagatga cgtagattta gcggtggtaa ataacactta tgccgggcag
    11701 gtaggcttaa atacccaaga tcacggtgta tttgttgagt ctaaagattc accgtatgtg
    11761 aatattattg tggctcgcca agacaataaa gatgcagcta atgtacaaaa ctttattaaa
    11821 tcttaccaaa ccgaagaagt gtaccaagaa gcacaaaaac actttaaaga tggtgtagta
    11881 aaaggttggt aatttagctg atttttactt aaatggcgat taagcgctag gttaatcgcc
    11941 attttcatct ttaaaatata ggaaggagca aacaatgaac tttaaaaaat tattaggtgt
    12001 ggcgttagta tctgccttag cacttactgc gtgtaaagat gaaaaagcac aagcacctgc
    12061 tacaacagct aaaactgaaa acaaagcccc attaaaagtg ggtgtgatga ccggccctga
    12121 agcccaaatg accgaagtgg tagtgaaaat tgcaaaagag aaatatggct tagatgtaga
    12181 gttagtgcag tttactgaat acactcaacc aaatgccgca cttcattcta aagatttaga
    12241 tgctaacgcg ttccaaacgg tgccttattt agagcaagaa gtgaaagatc gtggttataa
    12301 attagcgatt atcggcaata cgctagtatg gccaatcgcg gcttattcta aaaaaattaa
    12361 aaacatttcc gagttaaaag acggagcgac tgttgcgatt ccaaacaatg caagtaatac
    12421 tgctcgtgcg ttattattgc ttcaagctca cggtttattg aaattaaaag atccgaaaaa
    12481 tgtgtttgct accgaaaacg atattatcga aaacccgaaa aatatcaaaa tcgtacaggc
    12541 ggatacctca cttttaaccc gtatgttaga tgatgtagaa cttgcggtaa tcaacaacac
    12601 ttacgcaggt caagctgggt taagtccgga taaagacggt attattgtgg aatctaaaga
    12661 ttcaccgtat gtgaatttag tggtaagtcg tgaagataat aaagatgacc cacgcttaca
    12721 aacttttgtg aagtcattcc aaaccgaaga agtattccaa gaagcgttaa aattatttaa
    12781 cggtggtgtg gtgaaaggtt ggtaatcagt tggacggcat attctgccgt ccttttctta
    12841 aaaggagaaa cagataatga aaataatgaa attagccggt gcagttgcaa ttttctcgct
    12901 ttttttaacc gcttgtaatg ataaagccga aaagttgaaa gtcggtgtga tttccggccc
    12961 tgaacataag gtaatggaag tggcggcaaa aattgcaaag gaaaaatata accgtgatgt
    13021 tgaattagtg gtatttaccg attatgccac gcctaatgca gctttagata aaggcgatct
    13081 tgatttgaat gctttccagc ataaacctta tttagataac caaattcagg aaaaaggcta
    13141 taaattagtg ccggtcggca atacttttgt ttatccgatt gcggcttatt ccaaaaaaat
    13201 taaatcgctg gcagagttga aagatggtga tactattgca gtgcctaatg atccgactaa
    13261 tttagcccgt gctttaattt tattggaaaa acaaggttta attaagctgc gagcagatgc
    13321 aggcttaaaa gcaaccagtg tggatattat tgaaaaccct cgtaaattgg tgatccaaga
    13381 aattgaagca ccattattgc ctcgaacgtt ggacgatgtt gccttttcga ttattaatac
    13441 tacctacgca gggcaaaacg gtttaacgcc aaccaaagac ggaatattcg ttgaagacaa
    13501 ggattcgcct tatgtgaatt taattgttgc ccgtgaaaat aaccaacatt ctgaagccgt
    13561 aaaagatttg gtgaaagcct accaaacaga agaggtgtat aacaaagcga atgaagagtt
    13621 taaaggggcg atgataaaag gctggtaatt gaagataaat aaggcataga ttcctatgcc
    13681 ttactttttt gttacagacc tgctccggct cttaatgctt cggctttgtc agttttttcc
    13741 caaggaaaat gtttacgacc gaagtgtccg taggcagcgg tttcacggta gattggtttg
    13801 attaaatcta acattttgat taagccatac gggcgtaagt caaagttttg gtagattaat
    13861 ttcacaagta tttcgttgct cactttacct gtgccgaagg tttcgaccat aattgatgtt
    13921 ggggcagcca ccccgatagc ataagaaagt tggatctcac agcgatcggc taagcctgcg
    13981 gcaacgatat tttttgcgac ataacgagca gcataagcag cagagcgatc cacttttgac
    14041 gggtctttac cagagaatgc accaccaccg tggcgagccg caccaccgta ggtatctaca
    14101 atgattttac gcccggttaa accacagtca cccattggac cgccaatcac aaaacgaccg
    14161 gttgggttga tgaaatattt ggtgttttgg ctgagccatt cgcttggtag taccggtttg
    14221 atgatctctt ccatcacccc ttcaattaac tctttttgcg aaatatgctc tgcgtgttgt
    14281 gtagaaagca ctacggcatc aatgccgata attttattgt tctcataagc aaatgttaat
    14341 tggcttttag cgtcagggcg taaccagtcg agtttgcccg atttacgcac ttttgactgc
    14401 tgttccatta agcggtgtgc gtaagtaatt ggagccggca ttaacacctc ggtttcattg
    14461 gtagcgtagc caaacataat cccttggtcg cccgcacctt gctctaacgg atcggcacgg
    14521 tcaacgccct ggttgatgtc cggagattgt ttaccaatcg catttaatac cgcacaagag
    14581 tgagcatcaa agcccatatc agagtgagta tagccgatgt cgcaaatcac ttggcgggtt
    14641 aagttttcaa tatccaccca cgcagaggta gtgatttcgc cccctactaa tgccataccg
    14701 gttttcacat aagtttcaca agcaacgcgt gctttagggt cttgttttaa aatttcatct
    14761 aataccgcgt ctgaaatttg gtctgcgatt ttatccggat gcccttctga aaccgattca
    14821 gaagtaaata aattaattgc catttgtatt cctctctatc ttaaatatgg attattaata
    14881 gtaggggcgg gtccagtgcc cgcccataaa ttatataaaa tgtttggaca ggcacaggac
    14941 cggtccctat aaaatttgtt tggaagtgtt tccgtctaga cgtctataat atattttttg
    15001 aacgttttag gcaaatagaa tttgtttatt tggctaaata tgcccttaaa ccagccaacg
    15061 catttttggc atcttctacc cctaaatcgt acattgcttg tattttattt acatccgttt
    15121 caaggcgact gattttgact gttctgcttg gacgaatcac aaaaatctgt tgtttttcat
    15181 tgagttgaat aatctgctca acggcttgat tatattccgc atagcggttt tcccagcgtt
    15241 gcactaattt cggatatttt ttataaaaac gtttaaacag ccacatcgag cttggtttct
    15301 tacggtattc aagtgggcga gtaagcacca cgataatttt gtcatatccc atttcaataa
    15361 atttctgcac cggaatactg tctgacacac caccgtctaa atatttttta ccctcaattt
    15421 cgactatttt ggatacaatc ggcattgagg aagtagctcg aaaggcttcc atttgctcga
    15481 acatattgtc aattttgatg tattcgggct caccggtttc gacattggtt acagtaaccc
    15541 aaaagtccat accggaatcc tcaaaggctt gttggtcaaa cggctccagt ttggaaggga
    15601 gttcatagta agcaaaatcc cgattaacga tattgccggt ggtaagcaag ctgtgtaacc
    15661 ccatataccg cttatcttta gcgtattttt tgttgtatcg caacgcccta cctttttgtt
    15721 ttgaaggcaa attcacgcca aataacgcac cggctgaaac accgattgct ccatctatat
    15781 gaatatgttc atctaaaaat acatccaaca cgccggcagt aaaaatacct cgcaaaccac
    15841 cgccttccag cactaatcca actttcatat cattctccct gtaatttgtg tattataacc
    15901 cgaattatct tgctaggaga aatcacaatg gctgatgcag aatttatgga tttcctgtta
    15961 gatcaactaa gcccgatttc cggtttgcgt tcgaaaaaaa tgtttggtga atattgcttg
    16021 ttttttgata ataaaattgt cgctattatc agcaaagatt atcgcatttt tgtcaaagct
    16081 aatccgcaaa ccttgccgtt attcttagct gaaaatgccg aacaatttca ctattttgct
    16141 aaaggaaaag tgcaaaaaat gcactactgg agcattccgg aattagcgat tgaagaagcc
    16201 gatgaactgg tcaaatggat tcggctaggc ttacaagcgg tgtaatttag cgaaaatttt
    16261 gtaaaaaaac gctaaaatat gcccgcttgt tagggggggg acctaatatt tctatacttt
    16321 aagattttat atttatgaac caaaaaaaat tagtttcttt gattgtcgtt attgtgttgg
    16381 caatcatttc tcaaattttt agcttttttg ataaaaaaga gccttcaaaa gcacaagaaa
    16441 aatcgaccca gagtagtcag tctcatcaag tttcaacttc atcactaggg aactatgatg
    16501 tgcagatgaa aaacgaccgt ttgaaacagc atattgtaga taccgattat tacaccttag
    16561 cgttgtcgtg gtcgccggcg ttttgccaaa aacaaaagcg aaataatcac ggtaaaattc
    16621 cgtctcattt ggaatatcaa tgtggtggct cagcggattt tggttgggtt attcacggct
    16681 tatggccgca aagtggcaag gcaagaagtg ttgatgaaca ccctcgttat tgccaaggcg
    16741 atttgccgat ggtggcagag cagacgatta agcaatattt acctgaatcg ccgggggcaa
    16801 ctttgttgca aggcgaatgg gaaaaacacg gtgcttgtgc ctttaaccaa gcggatgatt
    16861 acttcgccaa acaaaaagag ctatttaatc agctcaaatt accggatacc gaaatgtcta
    16921 aattagcact gtttaactgg atgcgagcca ataaccctca tttgcaaaat gtctatttag
    16981 ggggtagcaa agatgagctg tatatttgct atgataaggc atttcaacca atggattgcc
    17041 cacgctaatg gcagatcttc gccaactgaa aatgcaggtt cagcgccaat taaagcgtga
    17101 tcttgaacgt gcaaatcact attttaatac cacttttacg ccaccaagta ttagctatgc
    17161 ggtgcgtggc gtaaaagccg gtgtggctta tcttgaacgc aacgaagtac ggtttaatcc
    17221 tgtgttattg caagaaaatg aacaggcatt tatccgccaa gttgtgccgc acgaactggc
    17281 tcatatcttg gtttatcaac attttgggcg ggtacaatct catggtaaag agtggaaaat
    17341 gatgatggaa accgtactgg gtgtgccggc ggaaatttac cattgttttg atacccaaag
    17401 tgtgcaacag caatttagtt accaatgtgc ctgccagact caccagcttt cgattcgccg
    17461 acacaatgcc gtgatgcgag ataaaaaaag ctatatttgc cgtttatgta aaactcaact
    17521 ccattttgta gaaaaaagtt aatgtggaga aaagttaaga gaatgttacg atttagctag
    17581 acattcctgt ttaaacgcct acaatttgcc gtagttttta tttttaattt tatttccgga
    17641 gtatttatgg aaacaattgt aattgtaggt ggcggtgcag gcggtttgga gcttgcaacc
    17701 tatctaggca acaaattagg gcgtaaacaa aaggcaaaag tggtgctaat tgaccgcaat
    17761 gcaacgcatt tatggaaacc actgttgcac gaggtggcaa ccggtactat tgatgaggga
    17821 actgatgcgt taagctatcg ggctcacgct aaaaatcata gttttgaatt tcagcaaggt
    17881 acgctggtgg cggtagatag agagaagaaa acggtttcac tcgccccgat ttataatgat
    17941 caaaaccaac ttctcgttaa agaacgccat atcagttatg acaaattagt gatcgcgatc
    18001 ggcagtaaat ccaatgattt tggcaccaaa ggggttgcag aaaattgcat tttcttagat
    18061 ggctcagagc aagcaaaaga tttccaaaaa cgaatgatgg agctgttttt acgtttttca
    18121 catagtgaaa ataaagatgt aaaaattgcg attgtggggg ggggagcaac aggtattgag
    18181 ctttcagcgg agctttacca cgtggtgaaa aatttaaatt cttacggctt tggtaaatta
    18241 aaccgtgcca gcttgaaagt cactttaatt gaggcaggcc ctcgtttgat tccggcatta
    18301 ccggagaaag tgtcggtttc tgctttccac gaactgaaaa aagccggtgt ttcggtgcgt
    18361 ttaggcacga tgattaccga ggctcgccca gacggattaa tgacccgctt aggtgagaaa
    18421 attgaggcgg atattatggt ttgggctgcc ggagtaaaag cacccgaaat cacccgtgaa
    18481 tttggttttg aaactaaccg cttgaaccaa attgagatta aagatacgtt acaaaccacc
    18541 gttgatgatt cggtatttgt cattggtgat tgtgcatcgt taatccaaga tggcaaacca
    18601 attccaccaa gagcgcaagc ggctcaccaa atggcaaccc gttgtggtaa aaatattgtg
    18661 gcgctgattg atgggaagga aatgaaaccg tttcgattta aagataaagg ctcgttgctt
    18721 tcattctcaa aattcggcac ggtgggcaac ctaatgggga atttagtgag cggtgatatg
    18781 tttattgagg gtaaaattgc gcgattggcg tatctctcgc tttatcgaat gcaccaagtg
    18841 gcattacacg gctgtgtcaa aaccggcttg attgtgttgg tagggcaaat taaccgtttc
    18901 ttaagaccaa caatgaaact acattaaaca aaaatccgca tcaaatgatg cggatttttt
    18961 atggcaaaat aacaagcggt tattttcttg cctatttttg caaatactca cgcctagttt
    19021 gctaatttaa tttccgccaa ccatttatca attaagcgtt tgccctcttc gctggaacct
    19081 gctttttggt aatcaatttc cacaaagtct aacgcatcgg ctttggttga ttgtggagca
    19141 acttcggcgt tgatgtttgt tggggtttgg taaacaccgt gttttttcca aggaatttcc
    19201 tgtgcttctt ttgaaagcac ccaatccata aagagtttgg cgttatctaa attacgggca
    19261 tttttgataa tgctgacacc gcctaacgca tagcccactt taccttccgg aataatggct
    19321 tcaataggcg cacctttttc tttttcagtg gcataactat gtacaaagcc taccgtaacc
    19381 gcactttcac cacgagcaag attgctggtg actagggtgc ttttcacata ttgcgagacg
    19441 ttattgttga gttttttcag ataatcaaag gttttctctt cgccccataa ggttgaaaga
    19501 gtggtcataa acgtataagt cgtgccggag ctatgaggat caggcagttg tacttcacct
    19561 tttaagcgag gatctgataa atcttcccat tttttcggtg cctcaatgcc tagtttggcg
    19621 agtttttcgg tattcacgcc taaccctaat acaagcatat aaacgataga ggtaaattta
    19681 ccttgttcac tttcaactaa ggatttaaat tgtggcagaa tttcagcttg ttttggtgaa
    19741 cggtaggctt ccagtaagcc taattcgcct gcttgaaagt gaggttcaat cgtgccgcca
    19801 taccaaatat ctgcctgagg gttcgatgct tccgctttta cttttccgaa aattgtaccg
    19861 gttccgccgt gaatgaattt agtttcgaca ttatatttgt gtgaaaattg cttggtcatt
    19921 tcttcacaaa cattattttg taccgagcaa taaacagtta aacgaccttc tgcttgtgct
    19981 gcttgagctg ataagaaaat gccagaggcg attaagatcg attttaatga gattaatttg
    20041 agtacgttca tcactttcct gaaaacataa aataaaaggt agtgaagtct agcatttcaa
    20101 agcgctggtt gaaaatagtt aatcgcaatg aagtagggca attgtgccta ttactttaat
    20161 atttaccaac tatttgcaag ttttagcaaa aaataaccgc ttgtagtagc tcttgctatt
    20221 ttgtcaaaat tcagaataat aaatgccttt agtctaatgt taaattttag ttatggaaaa
    20281 tagttggaaa tatgctttta aattaaccac gccgattttt atgggctatt ttgcggcagg
    20341 ggttgcttac ggaatgcttg ccaccaatgc cggaatgcct gcgtggttta cgattgtaat
    20401 gagttttacc gtggtatcgg ggacggcaca atatgcagcg attccatttt ttgtctccgg
    20461 tgttggggcg gtgtcggtct ttctcagtac gcttttaatg agcctacgtt ttagttttta
    20521 caccttaaat atgatggagc ataagccgat atccaaagcg aaaagtctgg tcgctattgc
    20581 aggtttaact gatgaaggct ttgcggttat cttcattacc gaaagagcaa aagcaagcat
    20641 cattctttaa aatcgctgtg ctatgtgtgg cttattggac attttccacg attgtcggtg
    20701 tgttgttagg cgattcagtg gcgaactata ttccgcattt ggattttgct ctgccttgtt
    20761 tgtttgcaat tttagcctat gagcaataca aaaaccaaaa acaatggaaa ccgattgtga
    20821 ttgcgttaat cggattttta cttgctcgtc agattaccga aaccagcgtg ttattggtgg
    20881 caattttagt agcgattgtc attgtggctt tattacctca atcttcttca atcaaactag
    20941 agaaaggagt agaaaatgag cacaaatgat attttgataa tgtttgcagc attagtgctc
    21001 ggctcgcaaa tttgtcgttt tttgccggaa tttttaccga aaaaggtgtt atcttcgccc
    21061 attttgcaaa aattaaacaa aatgttaccg cttgtgatta tgcttttatt gcttttaacg
    21121 agcctaaact ttcctaaaaa cggggaaggc tttaacttaa tattggcaca aattctggct
    21181 ttaataggcg tattaggtag ctatgtttgg cttaaaaata ccgttttaag cgtagcgttg
    21241 gggattttcg gtgtgaatgt atttttatcc ctgttaggat aaataccaaa ggtcatttta
    21301 taggagcgaa acaccttcgc tcaatcatta acagatgtgg ggcgaaaaat ttttcgcccc
    21361 ttatttatca caagcggtct ttttctttca attttttgca aattaaatcg ctaaatcaaa
    21421 cgcatagaag gcaactaaaa taatcgcaat gattactgta cctaggttta atttattggc
    21481 ttcgcctgaa attaagcgac caatcacaag ggtggcgaaa cctagcataa tgcctgtcac
    21541 aatgttggcg gttaatacga tgaacaccgc acagactaaa cctgacattg cacctacaaa
    21601 atcgctgaaa tctaatttag agacattact tagcattaat aagcccacat acattaatgc
    21661 cggtgcggtg gcatagcttg gtactaagaa tgcaagaggt tggaagaaca gcataaataa
    21721 gaaaccgata ccaaccacta cagcggttaa acctgtttta ccgccaacgg ctgtacctgc
    21781 tgccgattca atatacactg ccgctggtgc tgtaccaaat aagcccgata aaattgagcc
    21841 gaatgagtca gaagttaggg ctttatcgcc attgataatt tgtccgtctt tatctaataa
    21901 accggcttgc cctgcaaccg cacggatagt acctgttgca tcaaaaatag cggtcattac
    21961 taacgcaaac actaccggta aaatggcggt atttaatgct cccataatat caagatttag
    22021 gaattgcgaa ttttcaccaa aactcggcat tttgaaaaag cctgtgtatt ttacgttagg
    22081 gtcaaaaatt aaacttacga tggtaatagc aataatgacc cataaaatac tgccttttac
    22141 tttcagcttt tctaacccga taatggcggc taaaccgatt aatgacacta aaaccggaaa
    22201 tgaagtgaag tcccctaatt taaccggaat atccataccg ctattgacca ctaatttaac
    22261 gttagtggcg gcaataatca gtaagaataa accaatacca attccagcac cgtgggcaat
    22321 gctgcttggc aaattacgta aaatccaagt acgaataccg gtagcagaaa tcaaggtaaa
    22381 tactacgccc attaagaaaa tcgccccgag tgcaacagga atagaaactt gttgccctaa
    22441 tactaagcta aaggcagtaa aagcagtaag tgaaatcgca caaccgattg ccattggggc
    22501 atttgcccat aaaccgatta agatagagcc gagacctgaa actaaacagg tggcaataaa
    22561 taccgattcg gcaggaaagc ctgcttggct tagcattccc ggcacaacga tgacggaata
    22621 aaccatggct aaaaaggtag ttaaaccggc gatgatttct tgacgagcgt ttgaaccacg
    22681 ctcctcaaat ttaaataacg acataagaac ctcaaaagaa gaaaatgaaa ggggaaattt
    22741 taggatgcgg aattttacag aaatttaaaa taattagcaa acgtttgcgt aagaagtgaa
    22801 agaaaagcag gaataaatcc tgcttttgtt tgatggttag taatcgatga aagcgtcttg
    22861 atctgcccct tccttttcaa ctttaggctg aaccatatgt tcacgtttta gcccaagttg
    22921 tagtgccaca ccaattgcaa tgtagatgga tgaataagta ccgaaaccga taccgatcaa
    22981 taatgcaagt gagaaactat gaagcgttgg accacctaga tagaataagg ctagtaccac
    23041 aaagagggtg gttactgatg tcattaaagt acgcgaaagt gtttggctca aggaaatatc
    23101 gataatttca attgagctgg agcgacgaac tttagaaaag ttttcacgta cacggtcaaa
    23161 caccacaatt gagtcattta acgagtaacc gacaacagat aaaatcgctg ctacaaaggt
    23221 caagtcaatt tcaatctgta agaaagagaa gattccgatc gtcactaata catcgtgaaa
    23281 taaagccaca ataccgccta ccgctaaacg ccactcaaaa cgtgttccca cgtaaataag
    23341 tagcatagca agtgttgcca aagtggcata aatagcccct tgggttagct cttcccccac
    23401 gtttgggcca acgaactcaa tgctcttgat agtcgcattc ggatctaatt tttgatggat
    23461 catctccatc actttattcc caacagacat attaccggca ctggcggcaa gtcggatcat
    23521 taaatctttt tgactgcctg tggtttgcac taaggcattg ccgtagccgc tattttcaag
    23581 ctccgtacgc agttttgcta aatcagcagg ttgtgaaaat tgagtttcga ttactgtccc
    23641 gccggtaaaa tctaaccccc agttaaagcc ttttgtcgca atagtaaaaa tgcataaagc
    23701 agttacaatc agcgaaaaaa tgaatccgac atagcggtat ttcataaagg ggattaattt
    23761 ataaggcagt ttaatttctg ctacatcttg ttgtacattt tcaatagcca tttttccacc
    23821 ttaaatccaa agtttcttca cgcgtttgcc accgtaaacc cagtttacaa gcattcgagt
    23881 acctgtaatt gcggtaaaca tagaaataat tacccctaat gcaagcgtta ccgcaaagcc
    23941 ttttaccgga cctgtaccta caccataaag cacaagagaa gttaaaattg tggtgaggtt
    24001 agagtcaaaa atacttgaga atgcgccatt ataaccttcg tggattgctt gttgaattgg
    24061 gcgaccgtta cgaatctctt ctttgatccg ctcgtagatt agtacgttgg catcaactga
    24121 catacccaca gcaagtacga taccggcaat tcccggcata gtaagggttg caccgattaa
    24181 tgacattaaa ccaatcgtta agatcatatt cacaatcaag gcaagagagg caaatatacc
    24241 aaatacttta taaaaagcta gacagaacag gatcgttaag cctaagccta aaatactagc
    24301 ttccatacct tgtttaacat tatctgcccc aagagaagca ccgatagtac gctcttccac
    24361 aatcacgatt ggagcaatca acgcacctga acgtaataat accgcaaggt tttgagcttc
    24421 cgctggtgaa ttaatccctg taatctggaa ttgattacct aaacgggttt gaatggttgc
    24481 aacgttaatt acctcttggt gtttttctaa aatgacttta ccgttagcat ctttacgacc
    24541 tgaatcttta aattcggtat aaagtgttgc catcggtttt tggattgcca ttttagtaat
    24601 ttcacccatc atctcgccgc cttcggtatc gagtcggata ctcacttgcg gcaagccacg
    24661 ctcatcagtg cctgaggttg catcaataat atgttcaccc cctaacgaag gtttacggcg
    24721 taatacaact ggctcgccat cacgggtgtt ttgcagttct gaatcagccg gcacaatgcc
    24781 tttcgccgca gaggccacat tggcttcggt attgactaac cggaactcaa gggttgcagt
    24841 tgcccctaaa agctctttgg cacgagcggt atcttgcaca cccggtaatt caactacgat
    24901 acgatctgca ccttgacgct gaatggtcgg ctcagaaacg cccaattcct caacacgttt
    24961 acgcaaaata gaaaggtttt gttcgattgc catcgtgcga gattcagaaa gagcttgtgc
    25021 tgatggcgtt aaggtgactt tattatcatt taccaaaata tcaagcgttg ggtgagagag
    25081 acgcactaaa cgcactgctt tttcggcggt atcgttatcg gcaaattcaa cctcagttgc
    25141 aaaattttcg ccttttttga ccgcttgata ttgcaatttt tctttacgga aatcactacg
    25201 caggctgtct tgcaaagtgt cttgttgttt tgcaagcgcc gtgttcatat ccacttccat
    25261 taagaaacga acaccgccaa gcaagtccaa gcctcgcttc atcgggctac cgccaatgtc
    25321 agacaaccac tgtggcgttg ctggtgcaag gtttaaggca acagagtagt tattccccaa
    25381 tgcttctgag attttttctt tggcaggcaa ttgctgttcg ctgctttcga gacgaaccaa
    25441 aatcgagcca ttttctaaca cggcagattt cggctgaata ttcattgctg aaagtgtgga
    25501 ttgcacttga gacagtgtct caggtgtggc ttgttgccca cgagtaccgg aaatttgtac
    25561 cgaaggatca tcgccatata aatttggaag ggcatataaa gcaccaatgg cgatcacgaa
    25621 gatcaccatt aaattcttcc atagagggaa acggtttaac acaataactc cccgaatgtt
    25681 aaagttggat aagagggttg attaaagtga ttttaatgaa ccttttggta acaccgcaac
    25741 aacgaaatca cgtttgatgg tcacttcagt tgtgtcattt aatgcaatca cgatgttatc
    25801 gttatcagcg gtcactttgg taattttacc gattaaacca ccgctggtta atacttcatc
    25861 gccttttgtc aagcttgcca gtaaatcacg ttgttgtttt tggcgttttg cctgaggacg
    25921 ataaatcata aaatagaaaa ttgcaccgaa aataatcagg ataaagatca tttccattcc
    25981 gccaccttgt tgcataaaaa ctccttaaat gtttgagtgg tataaaaata agtgttataa
    26041 aaaacgcctg ttaaaatacc ataaattcaa tgaattaacg agtagaaaaa ttcatcgaac
    26101 gcttttcttt taaacaaaaa tttgagtaga atcagcactt ttagtttagg attaggttct
    26161 tggcgtgttc ttgcgctgac acaataggag aaaaaatgaa aaaattattc gctcaagcgg
    26221 ttatttttgg ggcttttttt gcaagttccg cttttgctca caatgtgcaa ttaaatcaag
    26281 cattacctgc tgtttcggtt gcaaaagacg gggaattggc agtggcaggt ggaaaggtta
    26341 gctataaaaa ttggcattca tcatctctgg caggtaaagt ccgtgttatt catcattttg
    26401 ccggtagaag ttcggtgaaa gaaaaaaatg aagatttgat gacagcaatt aaaaatgccg
    26461 gttttgatcg ctcgaaatac caaacggtga cgattgtaaa tgctgatgat gcgattgtcg
    26521 gcacaggggt atttgtgaaa aatagtgtgg aaaacggcaa acttgacaat cctcacagcc
    26581 aagtagtgtt agaccagcaa ggcagtgtga aaaaagcgtg gggcttaaaa gagaaagaga
    26641 gctttattgc agtgctggat aaatcgggta aggtacaatt tacctctgag ggcaaactct
    26701 ctgcaaaaca gattcaagaa gtgattgggt tggtgaaaac attaacgcaa taaccagtgt
    26761 ttttatgggc ggattttgta tccgcccatc attctagata ataaatcgat aacttatcgt
    26821 gtgatgatac tcttctcctg cttttgttat cccgccaaat tggagtaatt caggctggtt
    26881 tagtgaattt gggaaaaact caggctctaa tgctacacca gcgtaatctt catattcgcc
    26941 tttagtcaaa ttaggttgtc cttttagcca gttgccggtg taaagttgta atgctggcat
    27001 ggaggtgcgg agttccattt ttaagtcttc cacggctaaa caagcggtcg gattttcact
    27061 attttttgca agatgaaagg agtgatcgta accttttacc gctttttggt cgtcatcagc
    27121 caataaatct tgcccaattt ttttagcatt gcggaagtca aaactggtac tctccacttt
    27181 tttcagtgga gcattaggaa taccgctggc atcaaccggt aagtatccgt ctgcattaat
    27241 tatcagctcg tgatctaaaa tcgtctctga accttgcaaa ttaaaatagg cgtgattagt
    27301 taagcaaagc ggtgtatctt ggttagcaac ggcatcaaag ctgatttgaa gctcattctc
    27361 tgttaagcga tactccacaa aagctttcac ttcgccgcca aatccttcct cgccatctgc
    27421 aaaaaaataa ctaaatttga ccgcttgttc gctttggctt tcaagttgcc acactatttt
    27481 atctgcccca agctcgccac cgtggagatt atgttcaccg ttatttttag cgagctggta
    27541 ggttttgccg tttaagcgat attcggcatt ggcaatgcgg ttagcgtaac gcccaatggt
    27601 cgctccaaag aaagcggtct gtttttgcca attttttgca gacgtggtga ctaatacttc
    27661 ccgttcctcg ttcggcattt tgacaatgca cgaaagccaa gaagccccaa catcagaaag
    27721 ggtaatgcgt aaaaacgcat tttcaagggt gaataatttc atcggttgac cttaattacc
    27781 tagcacggca tagccgtact aggttaagca taattcagcc ctgctagggc tgaggttatt
    27841 tattttagag tagcggagct acttccttat gcttaacccg gtacgcttta gcgtgccggg
    27901 cattctcgca ccccatcgga ggcagtacaa acgtggaagg tttctttaat gccggtttgc
    27961 ttttcgtaat tgttagcaac ggcttggcgg agatcttcca ctttgtcatt tggcactagg
    28021 cacacgacac aaccgccaaa gccgccgcca gtcattctgg ctccgccgtt tttaccgata
    28081 gcttgttggg cgatttcggc taagcaatcc acttcagggc aggtgatttc aaaatcatca
    28141 cgcattgagt cgtgagattg agccatcaac tccccgagtt tgaccatatc attcgctttt
    28201 aaggcctcta ctgcttccag aactcgttga ttttcgctga tgatatgttt tgctcgttta
    28261 tacgccagct cattctcttc actaagttct gaggctcgtt caataaattg ttccgctgtg
    28321 acatcacgca attttgcttt gccgaagaag tgggcggcaa attcgcactg ttcacggcga
    28381 ctgttgtatt cgccggtaac caagtcgtgt tttacgttgg agttaataat cgcaatcgaa
    28441 tagccttgtg gcacaggggt tggggtgatt tctaaactac ggcagtcgat catcactaaa
    28501 tgatcttgtt gcccgagagc ggaggtgagc tgatccatat tgccgcagtt gcagccgaca
    28561 aatttattct cagcctcttg cccgattaat gcgatttgcg gtaaagaaag cggtaaatcg
    28621 ccgagtaatt gagcggtttt accaatcgac acttccaatg cggcggaaga acttaaacct
    28681 gatgacatcg gcacatcgct ggtaatggct aaatctgctc cttgtttgaa ttcggggtgt
    28741 ttggcttgaa taaatttcac tacgccacgc acataattcg cccatttttg ctcgcttggc
    28801 tcaatcgcct ggcttaaatc aaattcatct tgttggttga gatcaatggc gaacacccgc
    28861 catttgttgt cactacgttt ggcaaagcta accgctgtgc cgtaattgat ggcacaaggc
    28921 attacaaacc catcgttgta atcagtatgt tcgccgataa tattcactct gcccggggca
    28981 aataccgtgc gttctgcttt ataaccaaaa tgttgttcaa attgttgcaa agcgatttct
    29041 tgtggtttca tttcaattcc ttatttatct ttatagtgtt tggtatcact tactgcattt
    29101 aaacgttcgg cagcttgttc cggggttaaa tcacgttggc tttctgccat catttcataa
    29161 ccgaccataa atttacgcac tgttgccgaa cgcaagagcg gcggatagaa gtgagcgtgt
    29221 aattgccagt gagcattttc cttgccgtta aacggagcga agtgaaagcc cattgaataa
    29281 ggaaagctga tgttaaacag attgtcgtaa cgggtggtga gctttttcaa tgcaagggct
    29341 aaatcggctt tttccgcttc gtttaaatcg gtaatggatt tgaactgctt ggctttcggc
    29401 aacagcaagg tttcaaacgg ccaagccgcc cagtaaggca caaccgcaat ccaatcttcg
    29461 gtttctgcca caattcgctc ttgtttgtct aattcacgtt gtgcgtagtc aagcaacagt
    29521 ggcgagccat attgagcaaa atatttcgct tggcattcgt cttcaatcgc aatttcatta
    29581 ggtagaaaat tacttgccca aatttgcccg tgtgggtgag ggttagaaca ccccatcatt
    29641 gagcctttgt tctcaaaaat ttgcacccac tgataatgct ctttcagctc attcgcttgc
    29701 tcttgccaaa ctgttaccac atcagtaatt tcctcgatgg aaagttgcgg taaggtttta
    29761 ctatgatcag gcgaaaaaca gatcacacgg ctttcgcctt gtgtatgaga aatctgaaac
    29821 agcggatcat cattcggtat tggtgccggt gtgtctggta gcagggcaga gaagtcgttt
    29881 ttaaacacaa acggcttggt gtaattcggg ttttgttcgc cggtaatccg cttattgccg
    29941 gggcagaggt aacaacttgg gtcgtagctc ggtttttcat cttgcacgat ttcttcttgt
    30001 tgcccttgcc acgggcgttt tgcccgatgc ggagagacta aaatccattg attttttaat
    30061 ggattaaaac gacgatgtgg gtgatcgttg aggacaaatt cgctcatata aaactcctcg
    30121 aaattagtta tgtaaagagt atagcgaaag tcagacaaaa tgactgtgac atagctcaca
    30181 aaataatgat gtcaagcggt caaatttcag aaaaattttg caagttttcc tccattttgc
    30241 gatttaaggt agcttacgtg gaattgaatg tggcttttgg tagaatagat gaatatgcaa
    30301 gatattaaga caaaaataga tggaaagact gaataaattt gatgcttggt taatgaatca
    30361 taaacctaat gctaggttaa gcggagcgag tcgtttgttg acagagtttt ggttctttgg
    30421 gttaaaagaa gctagagcct gtttatttgc gggattattt tttattgcga tgttttcggt
    30481 accaaaaacg ggattatggg gcttacctcg ttatgatatt ttgctgattt ttgccatagt
    30541 ggtgcaaatt gcaatggtga aactggggct ggaaacgtgg gatgagctaa aagcaataag
    30601 cctatttcat ttggttggtt ttgtgttaga agtctttaaa aca
//
LOCUS       DANKBV010000012        29387 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.012, whole genome shotgun sequence.
ACCESSION   DANKBV010000012 DANKBV010000000
VERSION     DANKBV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 29387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29387
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.012"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            145..1467
                     /gene="pepP"
                     /locus_tag="PWY43_000447"
     CDS             145..1467
                     /gene="pepP"
                     /locus_tag="PWY43_000447"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDL2135356.1"
                     /translation="MDLAYLAKMPQEEFTARRERVLEQMQDNSALLVFTESEKRRNSD
                     SEYLFRPDSYFWYLTGLAEPKSAVLLVKSEGKTESIIFVRKKDPLMETWNGRRLGIEE
                     APKTLQLNAAFDIDDIETVLAKKLENLTACYCALGIQEWGDNILFATFEKMKVNRQKV
                     PTTLIDWQPMLSEMRLIKSEFEIALIQQACHISSMAHIRAMKQARPNRYEWEIEGEIQ
                     HEFSRFGARFPAYNSIVAGGENACILHYNENDQVLKGGDLLLIDAGAEFAYYAGDITR
                     TFPINGKFSEPQKELYELTLTMQKEAINLLVPNSSIKAANDKAVQILTQGLVRLGILN
                     GDVESLIEQKAYRQFYMHGLGHWLGLDVHDVGDYGTERDRPLQIGMVLTVEPGIYIPK
                     DADVPEQYKGIGIRIEDNLLITEYGNKNLSSGCPKEIADVEQLMQSLN"
     gene            1583..1768
                     /locus_tag="PWY43_000448"
     CDS             1583..1768
                     /locus_tag="PWY43_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDL2135357.1"
                     /translation="MYFEIYKDAKEEFRWRLKAGNHEVIATSQAYTTKQGCQKGIEAV
                     KKVTPETEVKDLTKAEA"
     gene            1897..3213
                     /gene="argA"
                     /locus_tag="PWY43_000449"
     CDS             1897..3213
                     /gene="argA"
                     /locus_tag="PWY43_000449"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDL2135358.1"
                     /translation="MQNTELALAQWFRQSTPYVNMHRGKTFVIMLDGDVIESPNFVNI
                     INEISLLHSLNIKLVIVFGARPQINQLLKQNHIEPEYYHNIRVTNPQSLEYVKQAVGK
                     VHYDFFARLSLRLPHSEVINVVSGNMILAQPIGVVDGVDYALSGKIRRINIESIKQQL
                     AQNSIVLLGPIAPSVTGEMFNLPFEDIATQVAIKLRADKLIGFCEEQGILDENGKPIP
                     DYLPQDGETLLQQLIERGEYHTPKARFLQAAITVCREGVKRSHLLSYKEDGSLLQELF
                     SRDGIGTQFSMESSEQVRIANSSDIPGLLDLIRPLEQQGILVKRSREQLEMEITNYTI
                     IERDGVVIACAALNPYPEEKMAEMACVAVHPDYRDSSRGDILLENIQKRAVQLGMEKL
                     FVLTTRTTQWFQERGFRLADAQDLPRYKRENYNYQRRSKVLILPLW"
     gene            3267..4154
                     /locus_tag="PWY43_000450"
     CDS             3267..4154
                     /locus_tag="PWY43_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL2135359.1"
                     /translation="MKKHQPIKGAMAIITAGVLFACINTLIPKLTSISPIDSSVIALV
                     QYLVAFIFLVPSMNNIGFFSSLKTEHFGQHCFRIFLSAIGIQCWTMALANQLPIWQGI
                     ALLMTSPLFVTIGSGLFLKEKVDKKRWIATAMGFVGAMIILEPWSDYFEMIALLPIAA
                     AFFWAGYSLMVKKLSRYDSPTTMVAYLFILITPFNLLIALTNLSPSGFSLPSWSDFGF
                     LILLGFFTALAQLAVAKAYSVADASYIQPFDFIKLPLNVLAGWLVFNWVPPGKLWLGA
                     AIIIAATIYITHAETKQSN"
     gene            complement(4234..4725)
                     /gene="ilvN"
                     /locus_tag="PWY43_000451"
     CDS             complement(4234..4725)
                     /gene="ilvN"
                     /locus_tag="PWY43_000451"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDL2135360.1"
                     /translation="MRRTLAVLLENESGALSRVVALFSQRGFNIESLTVAPTDDPSLS
                     RMTIVAQGEEQVLEQIEKQLHKLIDVFKVSNLSAYEHVEREVLLVKVRATGSSRDELK
                     RMVDIYRGQIVDLTPKLYTIQLSGSTEKLNAFIEAVKEETTIVEIVRSGVISLSRGDK
                     NAL"
     gene            complement(4718..6439)
                     /locus_tag="PWY43_000452"
     CDS             complement(4718..6439)
                     /locus_tag="PWY43_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDL2135361.1"
                     /translation="MKKLSGAEMIVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLGLNH
                     VLVRHEQGAVHMADGYARSTGKVGCVLVTSGPGATNTITGILTAYTDSVPLVILSGQV
                     PSSLIGTDAFQESDMIGISRPVVKHSFLVKNTEEIPEIIKKAFYIASTGRPGPVVIDI
                     PKDVVNPLNKYPYEYPQEVSMRSYNPTVQGHKGQIKKALKALLVAKRPVLYVGGGVIS
                     AECSPEITEFAQKLNLPVTCSLMGLGAYPASDKQYLGMLGMHGTFEANNAMHESDLIL
                     GIGVRFDDRTTNNLAKYCPNAKVIHVDIDPASISKTVQAYIPIVGSAKNVMEEFLSLL
                     GEENLAKNQVDLTAWWQQIDAWKARNCLAFEENSDVIKPQQVIRMIHKLTNGEAYVAS
                     DVGQHQMFAALHYGFDKPRRWINSGGAGTMGFGLPAAMGVKFAHPDATVVCVTGDGSI
                     QMNIQELSTAKQYDTPIVIVSLNNRFLGMVKQWQDIIYSGRHSQVYMNSLPNFAKLAE
                     AYGHVGIQIDHPSELEAKLTEAFSIKDKLVFVDVLVDETEHVYPMQVRGGAMNEMMLS
                     KTERTDA"
     gene            complement(6762..7442)
                     /gene="can"
                     /locus_tag="PWY43_000453"
     CDS             complement(6762..7442)
                     /gene="can"
                     /locus_tag="PWY43_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDL2135362.1"
                     /translation="MEKIERLFANNHAWATKMKDEQSDYFKELAEHQKPTYLWIGCSD
                     SRVPAEKLTGLGPGELFVHRNVANMVIHTDLNCLSVVQYAVDVLEIEHIIICGHTNCG
                     GINAAIAQKELGLINNWLLHIRDIWFKHSHLLGKLPYEDRSNVLTRLNVAEQVYNLGQ
                     SSIIKTAWEQGKSLSLHGWVYDVTDGFLIDQGVMATSRETLEITYRNAIAKITTEANE
                     KIVLNDEN"
     gene            complement(7556..9691)
                     /gene="pta"
                     /locus_tag="PWY43_000454"
     CDS             complement(7556..9691)
                     /gene="pta"
                     /locus_tag="PWY43_000454"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL2135363.1"
                     /translation="MSRTIILIPISTGVGLTSVSLGLVHALEQKGSKVGFLKPISQPI
                     SGEDTLDRSTTIIRAEQTTEVGEPFMLSEAETLIGQNQTDVLLEKVVERHQKLSKNNE
                     IVIVEGLIPTRKNSYANSINYEMAQALDAEIVLVAAPASDTPAQLKERVEAAASQFGG
                     RNNPNLLGVIINKFNAPVDDSGRTRPDLTEIFDSFQHSNNNVSEVEHLFAKSPIKLLG
                     CISWKAELIATRAIDVAKHLGASIINEGEIKARRIRGVTFCARNLPNMVDHFKAGSLL
                     VTSADRPEVLVAASLAVMNGIEIGAILLTGGYKIDSSIAKLCQQAFETGVPVFRIEGN
                     TWQTALSLQSFSLEVPVDDKERIAAIKEYMASKFNVGFIDEVSKAATRARRLSPAAFR
                     YQLTEYARQAKKRIVLPEGDEPRTVKAAALCAERGIAECVLLANPTDVQRVAEAQGVV
                     LGKGVTIIDPASIRENYVDRLVELRKAKGMTEVVAREQLLDTVVLGTMMLEANEVDGL
                     VSGAVHTTANTIRPPMQIIKTAPGSSIVSSIFFMLLPDQVLVYGDCAVNPDPTAEQLA
                     EIAIQSAESAKAFGIDPRVAMISYSTGTSGSGADVEKVKEATRIAQEKRPDLIIDGPL
                     QYDAAIMEDVARSKAPNSPVAGKATVFVFPDLNTGNTTYKAVQRSADLVSIGPMLQGM
                     RKPVNDLSRGALVDDIVYTIALTAIQATQ"
     gene            complement(9744..10952)
                     /locus_tag="PWY43_000455"
     CDS             complement(9744..10952)
                     /locus_tag="PWY43_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL2135364.1"
                     /translation="MSKNLILILNCGSSSLKFAILDPKTGDEKLSGLAEAFHLEDARI
                     KWKLNGEKGNADLGAGATHSEALTFIAEKLLSDELKAGIGAIGHRIVHGGEKFTSSVV
                     ITDEVIKGIEDATQFAPLHNPAHLIGIKEAFRIFPELKDKNVAVFDTAFHQTMPEHAF
                     LYALPYTFYKENGIRRYGFHGTSHFYITSQVAELAGKSVEQTNAIICHLGNGGSVSVV
                     RNGKCIDTSMGLTPLEGLVMGTRSGDLDPAIISFLYNNLGMSMDEIDTTLVKKSGLLG
                     LTGVTSDCRYAEDNYNDVSKPEAKRALDVYSYRLAKYIGSYMAILGDDHLDAIAFTGG
                     IGENSGLVRELTLNHLKLFGIKLDQERNLAARFGKDGVITADESTFKAVVIPTNEELV
                     IAQDTAKLAL"
     gene            11193..13154
                     /gene="cpdB"
                     /locus_tag="PWY43_000456"
     CDS             11193..13154
                     /gene="cpdB"
                     /locus_tag="PWY43_000456"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDL2135365.1"
                     /translation="MLNRRKFIQLGATAMLVLGTQPSAFAQTAKNKAGLRVIATTDIH
                     GFLTDFDYYKDAPTEKFGFTRTASLIKQARAEVKNSVLVDNGDLIQGNPISDYQSTKG
                     AKEGKPEPSIMALNAMQYEVGTLGNHEFNYGLSYLDNAIKQAKFPIVNANIVKPGTDE
                     PFFTPYVIQQKEIVDEKGNKHTIKIGYIGFVPPQVMIWDKANLEGKVEARDIVKTAQK
                     YVPIVKKEGADIVVALAHTGPSDEPYKEGAENCAFYLADVKGIDAVIFGHSHRLFPNK
                     EFANIAKGTMKGVPESMAGYWGNNISVIDLSLAENDGKWGVVDGRAELRPIYNTEEKK
                     ATVENHPEIVALLKETHEATRQFVSQPIGKATDNMYSYLALVQDDPTIQIVNQAQKAY
                     VENVVKGLPELAGLPILSAGAPFKAGGRKNDPTGYTEVDKGELTFRNAADLYLYPNTL
                     VVVKATGAELKEWLECSAGMFKQIDVASDKPQYLLDWTGFRTYNYDVIDGVSYQFDIT
                     QPARYDGECKLINPNSHRVVNLTFDGKPVDPKQEFLIATNNYRAYGAKFPGTGDKHIV
                     FAAPDENRQVLANYITTESKAHGQVSPKADNNWRIAPISSNAKLDIRFETSPTDKAKA
                     FIKENAQYPMEYIENDETGFAVYRIDLTK"
     gene            complement(13227..14705)
                     /locus_tag="PWY43_000457"
     CDS             complement(13227..14705)
                     /locus_tag="PWY43_000457"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL2135366.1"
                     /translation="MDSILGKKINSTAIPSLLCDFYKTSHRIQYPEGSQFLYSTFTPR
                     SNIYAPFVTRVVSFGFQAFILKYLIHYFNDNFFSRSQHDVVSEYTQFISDTLNIEDSG
                     EHIAQLHQLGYLPIRIKAIPEGKSVAVKVPLMTIENTHSSFFWLTNYLETLINVSLWQ
                     PITSASIAFAYRKALAEFSEQTCDDNSHLPFQAHDFSMRGMSSLESAESSGAGHLTSF
                     LGTDTIPAISFVTAYYGSQHLIGTSIPASEHSVMSSHGVDELPTFRYLMAKYPNNMLS
                     IVADTTDFWHNITVNLPLLKQEILARPESAKLVIRPDSGDFFTIICGNSSSPVEHERK
                     GLIECLWDIFGGSINQKGYKVLNPHIGAIYGDGVTYDKMIRILEGLTAKGFASSNIVF
                     GVGAQTYQRNTRDTLGFAIKATSITINGVEKAIFKAPKTDNGLKKSQKGRVKVISYDH
                     YVDELTALDDFSDDLLEVVFENGKLVKSTNFDEIRQNIAQQF"
     gene            complement(15034..16167)
                     /locus_tag="PWY43_000458"
     CDS             complement(15034..16167)
                     /locus_tag="PWY43_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis PLP-dependent protein"
                     /protein_id="HDL2135367.1"
                     /translation="MTKFNNIETTLVQIGNRTDPRTGAVAMPIILSTAYGRDGLGEST
                     GWDYTRTKNPTRAVLEQGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVY
                     GGSYRLFDFSHKNHGTLKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVAV
                     IAKIAKKHNLMLIVDNTFLTPALFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDS
                     DATKVNGQNLTERLFYFQNCAGAVLSPFDSYLAVRGMKTLALRMERHQSNATELAKFL
                     AEQPEIDSVLYSGKSGMLSFRLQKEEWVAKFLKAIKLITFAESLGGAESFITYPATQT
                     HMDIPEAERITRGITNNLLRFSVGLEHVEDIKADLLQAFKQLK"
     gene            complement(16184..16996)
                     /gene="dapB"
                     /locus_tag="PWY43_000459"
     CDS             complement(16184..16996)
                     /gene="dapB"
                     /locus_tag="PWY43_000459"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL2135368.1"
                     /translation="MTLKIGIVGAGGRMGRQLIQAVHNAQGVELGSAFERKGSSLVGS
                     DTGEVAGVGTLGVVISDDVAQNVAGFDLLIDFTRPEGTLEHLDICVAHHKQMVIGTTG
                     FDDAGKAAIKAASEKIGIVFASNYSVGVNLVFKLLEKAAKVMGDYCDIEVIEAHHRHK
                     VDAPSGTALSMGEHIAKTLGRDLKTHGVFERNGITGERKHDEIGFATIRAGDVVGEHS
                     VWFADEGERVEIAHKASSRMTFANGAVRAAKWLNGKQNGLFDMTDVLDLNNL"
     gene            complement(17047..17424)
                     /locus_tag="PWY43_000460"
     CDS             complement(17047..17424)
                     /locus_tag="PWY43_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135369.1"
                     /translation="MGIKVLFGTKISAELQYLPEKDLVKILQFKQHVEIYGLENLAGR
                     NKSSDDVPTNDPYWAEKVAKAQKYSLWHYHIGIPEYDTSQGFGNYTSEYILHYVKGEN
                     FIQIVDFTSHPPFKLPSESYLQN"
     gene            complement(17414..17887)
                     /locus_tag="PWY43_000461"
     CDS             complement(17414..17887)
                     /locus_tag="PWY43_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135370.1"
                     /translation="MMKNLPLSMADTLAVIYPAFDFAADDKPATEQQISLLNFRITMA
                     NQLLIACIETIEKNPNFWAEIDLNDFKKYLNQNKVVIFDTQQYFTDGSRTMAILDNMK
                     SNIRKIEQQLSQATAFNFDLERMKERLNDTFHEVPKDITNVNEFRNWLKGVANGH"
     gene            complement(17955..19043)
                     /gene="rlmM"
                     /locus_tag="PWY43_000462"
     CDS             complement(17955..19043)
                     /gene="rlmM"
                     /locus_tag="PWY43_000462"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDL2135371.1"
                     /translation="MFNKLALYCRAGFEKEVAGEITDKAAQQGIFGFANLKENSGYVI
                     FECYQAGEADRLAQALKFEQLIFVRQMLVVGDLLQNLSENDRISPILAQYAELNPRQS
                     SDVFVETPDTNEAKELLTFCRKFTVPLRSALKKQGWLGKSENMQGSVSLHILFTRPGA
                     CYVGYAYNHNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPQAEEKKRFNDTMVG
                     VDLGACPGGWTYQLVKRGVFVYAVDHGKMAASLHETGRIEHCPEDGFKFQPPKRKQID
                     WLVCDMVEQPIRISKLIAKWLTNGWCRETIFNLKLPMKKRYQEVQLCLNFLEEELTKH
                     GFWFKMQAKHLYHDREEITVHIAVMGQK"
     gene            complement(19043..19435)
                     /locus_tag="PWY43_000463"
     CDS             complement(19043..19435)
                     /locus_tag="PWY43_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL2135372.1"
                     /translation="MKNKFVAIAAFSGLFTVAFGAIASHALSAQLSPKALGWIETGIK
                     YQMFHTLAILALGLFQIANFGQNPPVCRAKAFNIIGSNWALGILLFSGNLYLLALNVP
                     TIHWLTPIGGLCFMLGWIALIYISVRKA"
     gene            complement(19542..21053)
                     /locus_tag="PWY43_000464"
     CDS             complement(19542..21053)
                     /locus_tag="PWY43_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL2135373.1"
                     /translation="MTTLFQQFSPTVIKQQWFSNVRGDVLSGLVVALALIPEAIAFSI
                     IAGVDPQIGLYASFCIAVIISFVGGRPAMISAATGAMALVLAGLVRDHGLQYMLAATL
                     LTGVLQIIAGWLKLGSLMRFVARDVVIGFVNALAILIFMAQLPELQNTTWHTYALVVA
                     GLAIIYLFPYVPVIGKIIPSPLVTIIALTLFTLWQGIDVRTVGDMGQLPDTLPVFLLP
                     DIPLNLDTLWIILPYSFSLAAVGLLESLMTATIVDEMTETDSDKNRECKGQGIANIIS
                     GLFGGMAGCAMIGQSVINVKSGGRTRLSTFLAGFLLLIFVVFLSDILKVIPMAALVAV
                     MIMVSIGTFNWQSLEELKTHPIGFNVVMLVTVAIVVYTHNLALGVFSGVLLSALFFAN
                     KIGRYMAVKTSAIEKEELRYDVIGQVFFASSDDFIAQFDFTRSELKKVHIDLTHAHFW
                     DVTAVSALDKVVLKYRKSDVEVEIIGLNEASKTLVDRFGVHDKSEDVDIILNH"
     gene            complement(21208..23565)
                     /locus_tag="PWY43_000465"
     CDS             complement(21208..23565)
                     /locus_tag="PWY43_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HDL2135374.1"
                     /translation="MNTALEKGKFVFLDKPKNTITTAAHWGALNVTVENGKVVKSEGS
                     LAKNHQNELQDVVADQLYSPVRVKYPMVRKGYLEGNKDTTLRGRDKWVRISWDKAFEL
                     VSGEIQRVREQYGSESIFGGSYGWYSCGSLHASRTLLQRYLNVTGGFTNTKGDYSTGA
                     VQVIMPYVLGNIEVYAQQTSWHTIVESTELLVIWSSNPLNTLRIAWTSAEPEGIDYLA
                     KLKASGKRIICIDPVKSETCQFLEAEWLPINTGTDVALILGIAHTLITENKHDHDFLT
                     KYTVGFEQLADYVLGKADNLPKTAEWASQICGIPEEVIKTLANDFVTHRTMLMSGWGL
                     QRLQHGEQSHWALVTLAAMLGQIGLPGGGFGLNYRYSTNGLNVNSGVRLGAINAGKPN
                     PDMPFFPVSRIADALLKPNQPYDYNGQTLHYPNIKLVYWAGGNPFAHHPDTNYLVKAW
                     QQPETVIVNECYWTPTARMADIVLPATTSYERNDLTVAGDYVAKHIIPMKQVVEPQFE
                     AKNDYDIFAELAKRAGKEQAFTEGKSEMEWLKEFYQVAFEAGEKANTPLLDFEAFWAE
                     NKVLSFEAEHQNNTFVRYQDYRTDPIKHKLATPSGKIEIFSETIAKMNYEDCKGHPTW
                     FEHHEYSSKTNEAEPLALVTPHIKYRLHSQLAYSSLRQYYEVNGREPMLIHTKDAADR
                     GITSGDIVRIFNKRGQVLAGAVVTDGIIQGTVALYEGGWYDPQDLGKTDTPLCKNGNP
                     NVLTRNEGSSKLGQGNAPNTTTVQVEKYVGDAPEVTVFKAPFFSE"
     gene            complement(23701..26307)
                     /gene="acnB"
                     /locus_tag="PWY43_000466"
     CDS             complement(23701..26307)
                     /gene="acnB"
                     /locus_tag="PWY43_000466"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDL2135375.1"
                     /translation="MENFIENYQQQVDERAALGVVPQPLSAEQTAQLVELLKNPPAEQ
                     AVFLLDLFTNRIPAGVDEAAYVKAAFLADITKGVATSPLISPAHAVKLLGTMQGGYNI
                     ETLLSALDNPELAPIAVEALSKTLLMFDNFHDVKERAEQGNPFAQQVLESWANADWFT
                     SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNERDGIFPDENG
                     HTGPIKQLEALKEKGFPLAYVGDVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVV
                     LGGKIAPIFFNTLEDAGSLPIEVDVTNLNMGDVIDIYPYASKICKHNSDEVLAEFELK
                     TEVLLDEVRAGGRIPLIIGRGLTHKARVALGLPESDVFIKPQSVSESNKGYTLAQKMV
                     GRACGVEGVRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPVDVITHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGTMQPGITLRDLVHAIPYYAIQQDLL
                     TVEKKGKKNIFSGRILEIEGLEDLKIEQAFELSDASAERSAAACTIKLNKEPIIEYLN
                     SNITLLKWMIAEGYDDKRTLERRIANMQAWLDNPELLEADENAEYAAVIEINLDEIKE
                     PIVCVPNDPDDARLLSQVQGDKIDEVFIGSCMTNIGHFRAAGKLLSQAQGELPTKLWI
                     APPTKMDAGLLTEEGYYSIYGKSGARVEMPGCSLCMGNQARVANNANVVSTSTRNFPN
                     RLGQGANVYLASAELAAVSALLGKLPTPQEYLDYMNNLQKDKDDIYRYMNFDQINSYV
                     KKADNVIVQVAV"
     gene            complement(26327..27574)
                     /gene="icd"
                     /locus_tag="PWY43_000467"
     CDS             complement(26327..27574)
                     /gene="icd"
                     /locus_tag="PWY43_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDL2135376.1"
                     /translation="MQTQVQIPQGETIQLNQDGSLQVPDNPIIPFIEGDGIGVDVTPA
                     MKAVLDAAVEKAYQGKRKIAWMEIYAGEKANKLYGENTWLPDETLELIRQYHVAIKGP
                     LMTPVGGGIRSLNVAMRQGLDLYNCLRPIRYYDGTPSPVKHPELVDMVIFRENSEDIY
                     AGVEWVAGSPEANKVIEFLQQEMGVKKIRFTEDCGIGIKPVSKQGTQRLVRAALQYVI
                     DNNRKSLTLVHKGNIMKFTEGAFKEWGYQVAEEFGAKLIDKGPWMSLTNPKTGEEIII
                     KDSIADAFLQEILLHPTEYDVIATLNLNGDYISDALAAQVGGIGISPGANIGFEAAIF
                     EATHGTAPKIAGQNKGNPGSLILSGEMMLRHLGWVEAADLVVKAVSKTIANKTVTFDF
                     AEMLEGVTLRSTSEFAQDIIANM"
     gene            27808..29088
                     /locus_tag="PWY43_000468"
     CDS             27808..29088
                     /locus_tag="PWY43_000468"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005762304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDL2135377.1"
                     /translation="MSKATLTLETGEKLELNMQKGTLGYQTIDIQPFLKHKLFAYDPG
                     LVSTAVCQSNITYVDGDEGVLLYRGYPIDQLAKHSDYLEVGYMLLFGERPTKEEYKDF
                     VQLIKKHTLVHEQLTKFFSGFRRDSHPMAVMCGVSGALAAFYHDAIDVNNQAHRELTA
                     IRLLAKIPTLAAMCYKYSIGQPFMFPQNHLSYAGNFLYMMFATPCEPYEVNPVLERAL
                     DRIFILHADHEQNASTSTVRTAASSGANPFACIAAGIASLWGPAHGGANEACINMLEE
                     IGTVDRIPEFIARAKDKNDPFRLMGFGHRVYKNYDPRAKVMRETCHEVLKELNIDNPL
                     FEVALELERIALSDPYFIEHKLYPNVDFYSGIVLKAIGIPTSMFTVMFALARTVGWIA
                     HWKEMYLQGNIKIVRPRQIYTGHTKRDFEPMDKS"
     gene            complement(29161..29331)
                     /locus_tag="PWY43_000469"
     CDS             complement(29161..29331)
                     /locus_tag="PWY43_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HDL2135378.1"
                     /translation="MEYKGEQLKSNEDSKEKALIGNLWEKLSQGKGLFIMAVKSDENG
                     QDVRTQLLNKLA"
BASE COUNT         8759 a         6211 c         5693 g         8724 t
ORIGIN      
        1 gttatattta atcttaatct gttttgtttt tgcaatcaag atctcaaata taatctttca
       61 tctgttttgg ttaggctgaa tttatgctgc gtttgtgcta aaataaggca ggtttcgata
      121 tagatataat aaaaaggaga aataatggat ctcgcttatt tggctaaaat gccacaagaa
      181 gaatttaccg cacgccgtga gcgtgtgctt gagcaaatgc aggataattc tgcgttattg
      241 gtttttaccg aatcggaaaa acgccgtaat agtgactctg aatacttgtt tagacctgat
      301 agttactttt ggtatttaac cggtttggca gaacctaaat cagccgtatt gctggtaaaa
      361 agcgaaggta aaacggaaag tattattttt gtgcgtaaaa aagacccttt aatggaaacc
      421 tggaatggca gacgtttagg gattgaggaa gctccaaaaa cgttacagct taacgcagct
      481 tttgatattg atgacattga aaccgttctt gcaaaaaaat tagaaaatct gaccgcttgt
      541 tattgtgcgt tagggataca agagtggggc gataatattt tgtttgcaac atttgaaaaa
      601 atgaaagtca atcggcaaaa agtgccgaca accttgattg attggcagcc aatgttatcg
      661 gaaatgcggt tgattaaatc agaatttgaa attgccttaa ttcagcaggc gtgtcatatt
      721 tcttcaatgg cacatattcg agcgatgaaa caagctcgcc cgaatcgtta tgagtgggaa
      781 attgaggggg aaattcaaca cgaatttagc cgttttggag caaggtttcc ggcatataat
      841 tcaatcgttg ccggcggtga gaatgcctgt attcttcact acaatgaaaa tgatcaggtg
      901 cttaaaggcg gcgatttatt actgattgat gcaggggcgg aatttgcgta ttacgccggc
      961 gatattaccc gaaccttccc tattaatggg aaattctccg aaccgcaaaa agagctgtac
     1021 gaattaacct taactatgca aaaagaggcg attaatcttc ttgtgccaaa cagctcgatt
     1081 aaagcggcaa acgataaggc tgtacagatt ctcacgcagg gcttggttcg cttaggaatt
     1141 ttaaacggtg atgtggaaag cctgattgag caaaaagcct atcgccaatt ttatatgcac
     1201 ggattagggc attggctagg gctagatgtt cacgatgtgg gggattacgg cacggagcgg
     1261 gatcgtcctt tgcagattgg tatggtgtta actgttgaac ccggtatcta tattcctaaa
     1321 gatgcggatg taccggagca atataaaggt attgggattc ggattgaaga taatttgctg
     1381 attaccgaat acggcaacaa aaacttaagc agcggttgcc ctaaagaaat tgcggatgtt
     1441 gagcagttga tgcaatcgtt aaattaattc gcaaattttt gtaaaaaaat gaccgcttgc
     1501 tttgatattt tggggaattt agcttaagct aacccgacac aaacacagtg tttgggcaat
     1561 cacaacacga aggagtgttt atatgtattt tgaaatttac aaagatgcaa aagaagaatt
     1621 tcgttggcgt ttaaaagcgg gtaaccacga agttattgca accagccaag cctatacgac
     1681 taagcaaggt tgtcaaaaag gcattgaagc agttaaaaaa gttacacctg aaacagaagt
     1741 aaaagattta accaaagctg aagcataatc tataaagccc tgatattatt tcagggcttt
     1801 gttatttatg caaatataat gcttaattat tcaatttaag gcttgcggat ttttctaaaa
     1861 aagataatat gcgtttatat tataaagagg aaatatatgc aaaacacaga actcgcatta
     1921 gctcaatggt ttaggcaatc tacaccttat gttaatatgc acagaggcaa aacctttgtg
     1981 attatgcttg atggcgatgt tatcgaaagc ccgaattttg tcaatattat caatgaaatc
     2041 agtctcctgc acagcctgaa tatcaaatta gtgattgtat ttggggcaag accgcaaatt
     2101 aaccaattgt taaaacaaaa tcatattgag ccggagtatt accacaatat ccgtgtgact
     2161 aatccacaat cgctagaata tgtgaaacaa gcggtaggga aagttcatta tgatttcttt
     2221 gcccgccttt ctttgcgttt accccattct gaagtgatta atgtagtcag tggtaatatg
     2281 attttagctc agccgattgg cgtggtggat ggggtagatt atgcactaag cggtaaaatc
     2341 cgtcgtatta atattgagag cattaagcag caactggcac aaaattcgat tgtattactt
     2401 ggtccgattg ccccgtctgt taccggtgaa atgtttaatc tgccgtttga agatattgcc
     2461 acacaggttg caattaaact tcgtgccgat aaattaatcg gtttttgtga agagcagggg
     2521 attttagatg aaaacggcaa gccaattcca gattatcttc cgcaagatgg cgaaaccttg
     2581 ttacagcagt taatcgagcg tggcgaatat cacaccccga aagctcggtt tttgcaggcg
     2641 gcgatcacgg tatgccgaga gggagtgaaa cgctcgcatt tattaagcta taaagaagat
     2701 ggttcattgc ttcaagagtt attctctcgt gatggtattg gcacacagtt ctcaatggaa
     2761 agctcggaac aggtacgtat tgctaattcc agtgatattc cgggattatt ggatttaatc
     2821 cgcccacttg aacagcaggg cattttagtc aaacgctctc gagaacaatt agaaatggaa
     2881 atcacaaact acaccattat tgagcgtgat ggtgtggtga ttgcttgtgc ggcacttaac
     2941 ccgtatccgg aagaaaaaat ggcggaaatg gcgtgtgtgg cggttcaccc tgattatcgt
     3001 gattcctcaa gaggggatat tctactggaa aatattcaaa aacgggcagt gcaactggga
     3061 atggagaaac tctttgtgct gactacaaga accacacaat ggttccaaga gcgaggattt
     3121 aggctggcag atgcccaaga tttacctcgc tataaacggg aaaattacaa ttatcaacgt
     3181 cgttctaaag tattaatttt accactttgg taaaatattc aatgtttttg accgcttgtt
     3241 accaagagaa ctataaaagg agagctatga aaaaacatca acccatcaaa ggggctatgg
     3301 caattattac cgcaggtgta ttatttgcct gtatcaatac cttaattccc aaattaacgt
     3361 ccatttcacc aattgattcg agtgtgattg cactggtgca atatttagtc gcctttattt
     3421 tcctcgtacc cagtatgaat aatatcggtt tcttctcttc attaaaaacc gagcattttg
     3481 ggcaacactg ttttcggatt tttctatctg cgattggtat tcaatgttgg acaatggcac
     3541 tcgctaacca attacctatt tggcagggca tagcgttatt gatgacttcg ccattgtttg
     3601 tgaccatcgg ctccggtctg tttttaaaag agaaagtgga taaaaagcgt tggattgcaa
     3661 ctgcaatggg gtttgttggg gcaatgatta ttctagagcc ttggagcgat tattttgaaa
     3721 tgattgcact gttgccgatt gctgctgctt ttttctgggc aggttattcg ctaatggtga
     3781 aaaaactctc tcgctacgat agcccaacta cgatggtcgc ttatctgttt attctaatta
     3841 cgccttttaa tttgttgatt gcactcacga accttagccc tagtgggttt agtctgcctt
     3901 cttggagcga tttcggcttt ttaatcttac tcggtttctt tacggcgtta gcacaactgg
     3961 cggtagccaa agcctacagt gtagcagatg cttcctacat tcagccgttt gattttatta
     4021 aattgccgct gaatgtatta gccggttggc ttgtatttaa ctgggtgcca ccgggtaaat
     4081 tatggctagg ggcagcgatt attatcgctg cgaccattta tattactcac gctgaaacta
     4141 aacagagtaa ttagcaacaa attcacgtta cataaaaaat ccctttatct ttaagataaa
     4201 gggatttgca aattttaagc taaaattgac cgcttacaga gcgttcttat caccacgaga
     4261 taggctaatc acgccggagc gaacaatctc tacaatcgtt gtttcttctt taactgcctc
     4321 aataaatgca ttgagcttct cggtagaacc ggataattga atggtgtaaa gtttcggtgt
     4381 taaatctacg atttgcccac ggtaaatatc taccatacgt ttcagttcat cacgagatga
     4441 acctgtagct cgcactttta ccaataacac ttcacgctca acgtgttcat aagcacttaa
     4501 gttactcact ttaaacacat caatgagttt gtgtagttgc ttttcgattt gttctaacac
     4561 ctgttcttcg ccttgcgcga caattgtcat acgggaaagg cttggatcat cagtaggggc
     4621 aacggtcaag ctttcaatgt taaaaccacg ctgcgagaaa agggcaacta cacgagataa
     4681 tgcacctgat tcattttcta ataatacggc taatgttcta cgcatctgtt ctctccgttt
     4741 tacttaacat catttcattc attgctcctc cacgcacttg cattgggtaa acatgttccg
     4801 tttcatcgac caatacatca acaaatacaa gcttatcttt aattgagaat gcctcagtta
     4861 attttgcctc caattcagaa gggtggtcga tttgaatccc aacgtggcca taagcctctg
     4921 ctaatttagc aaagttaggt agagaattca tatacacctg tgaatggcgg ccagagtaaa
     4981 taatatcttg ccactgtttc accataccta agaaacggtt atttaagctg acaatcacaa
     5041 tcggtgtgtc gtattgcttt gcggtggaaa gttcctggat attcatttgg atactaccat
     5101 caccggttac acaaacaaca gtggcatcag ggtgggcaaa ttttaccccc atcgcagcag
     5161 gtaagccaaa gcccattgtg cctgcaccgc ctgaattaat ccaacgacgt ggtttatcga
     5221 acccataatg caatgcagca aacatttgat gttgtccaac atctgaggca acataagcct
     5281 ccccattggt caacttgtgg atcattctaa tcacttgttg tggcttaatc acatcactgt
     5341 tttcttcaaa ggctaagcaa ttacgagctt tccaagcatc aatttgttgc caccaagcgg
     5401 ttaaatctac ctgatttttt gcaagatttt cctcacctaa taaagataaa aattcttcca
     5461 ttacattctt agcactgcct acaattggaa tataagcttg tactgttttg gaaattgagg
     5521 ctggatcaat atccacgtgg atcacttttg cattcgggca atattttgct aagttatttg
     5581 ttgtacgatc atcaaaacgt accccaatac ctaaaatcaa atcactttcg tgcattgcat
     5641 tatttgcctc aaaagtgccg tgcatgccta acatacctaa atattgctta tcagaggcag
     5701 gataagcacc taatcccatt aatgaacagg ttacaggtag atttagcttt tgtgcaaact
     5761 ctgtaatttc tggcgaacac tctgcactaa ttacaccacc gcctacataa agcactggac
     5821 gttttgctac taatagtgct tttaatgctt ttttaatttg ccctttatgc ccttgaactg
     5881 ttgggttata agaacgcatt gatacttctt gcggatattc atagggatat ttatttaatg
     5941 gattgactac atctttagga atatcaataa ctaccgggcc tggtcgaccg gttgatgcaa
     6001 tataaaaggc ttttttgata atttcaggaa tttcttctgt attttttact aagaaactgt
     6061 gttttacaac cggacgagaa atgccgatca tatcactttc ttggaatgca tctgtcccaa
     6121 ttaaacttga tggaacttga cccgacagta ttactaacgg aacagagtcg gtataagcag
     6181 ttaaaatacc agtaattgta ttcgttgctc ccggacctga agtcactagc acacagccga
     6241 ctttacctgt tgaacgggca tagccatcag ccatatgtac agcaccttgc tcgtgtcgaa
     6301 ctaaaacgtg attcaatcca agtgtatgaa ttgcatcata aatatcgagt acagagccac
     6361 ccggatagcc gaaaacgtat tctacgcctt catcctttaa ggactgtaca atcatttctg
     6421 caccagaaag ttttttcatt gaatcctcca acaaaatagc attttcttct ctaagatggt
     6481 tggattttgc agtaaaattt tattgatgtc tagttcttat tatgggatca aatgctagat
     6541 ttgctagatt tatattcttt tttatcttaa aaagttggga ttttataaaa ataaatctat
     6601 atatttgtat gtttttgatt tttaaatcta tattttacac ttttgctgaa atttaaaact
     6661 tttttgacga taacaggaat caggaaaata aaaaaccatc aaacaatggt tttttataaa
     6721 aagattgtaa aaaaatcgat aaatttaacc gcttacaatg gctaattttc atcgtttaaa
     6781 actatttttt cgtttgcttc ggttgttatt ttagctattg cattacgata ggtaatttct
     6841 aatgtttctc ggctagtagc cataacacct tggtcaatca agaaaccatc tgtaacatcg
     6901 tatacccaac cgtgtagcga gagggattta ccctgttccc aagcggtttt aataatagag
     6961 gactgaccta aattataaac ttgttctgca acatttaatc gggtcaagac atttgatcga
     7021 tcttcatagg gaagtttgcc tagtaaatgg ctgtgtttaa accagatatc acgaatatgt
     7081 aacaaccagt tattaattaa accaagttct ttttgtgcaa ttgccgcatt gataccacca
     7141 caattagtgt gaccacagat aataatatgc tcaatttcta atacatctac cgcatattga
     7201 acaaccgaca gacaattaag atcagtgtga atcaccatat ttgctacatt tcgatgaaca
     7261 aataattctc cggggcctaa ccctgtgagt ttttcagcag gcacacgact atcggaacag
     7321 ccaatccata aataggttgg cttttgatgt tccgctaatt ctttaaaata atccgattgt
     7381 tcatctttca tcttagttgc ccaggcgtgg ttattggcaa ataagcgttc aattttttcc
     7441 ataaattctc ctttttaaca ctaatcttga ttatatgatt aaattgaaaa gtcgggtagg
     7501 taaaaaataa aaatgccctt agttttaagg gcatttggtt tcatttggga gggaattatt
     7561 gcgttgcttg aatcgcagtt aacgcaatag tataaacgat gtcgtcaact aaagccccac
     7621 gggacaagtc atttacaggt ttacgcatac cttgtaacat tgggccgata gagactaaat
     7681 ctgccgaacg ttgtaccgct ttataggtgg tattaccagt atttaaatcc gggaagacaa
     7741 atacagttgc tttacctgct acaggtgaat ttggtgcttt tgagcgggca acatcttcca
     7801 taatcgccgc atcatattgt aatgggccat caatgattaa atctggacgt ttttcttggg
     7861 caatacgagt tgcttctttc actttttcta catccgcacc tgaacctgat gtaccggtag
     7921 agtaggagat cattgcaaca cgagggtcaa taccaaaggc tttggcagat tctgctgatt
     7981 gaatcgcaat ttccgctagc tgttctgcag ttgggtcagg gtttaccgca cagtcaccat
     8041 ataccaatac ttgatcaggt agtagcataa agaagataga agatacaata gagcttcccg
     8101 gtgcagtttt aatgatctgc atgggtgggc gaatagtgtt agcggttgtg tgaactgcgc
     8161 ctgagactaa accgtccact tcattagctt caagcatcat tgtacccaat acaactgtat
     8221 ctaaaagttg ctcacgagca acaacttcag tcattccttt tgctttacgc aattcaacta
     8281 agcgatcaac ataattttca cgaatgctcg cagggtcaat aatcgttacg cccttaccta
     8341 ataccacgcc ttgtgcttca gcaacacgtt gtacgtctgt tgggttagct aataacacac
     8401 attctgcaat gccacgctca gcacataatg cagccgcttt cacagtacga ggctcatctc
     8461 cttcaggtaa aacaatgcgt tttttcgctt gacgggcata ttcggttaat tggtaacgga
     8521 atgctgcagg tgataaacgt cttgcacgag tagcggcttt agaaacctca tcaatgaaac
     8581 ctacattaaa tttgcttgcc atatattctt taattgcagc aatacgctct ttatcatcta
     8641 caggcacttc taagctgaag ctttgtaggc ttaatgcggt ttgccaagtg ttaccttcaa
     8701 tacggaatac aggcacaccg gtttcaaatg cttgttggca aagttttgca attgagctat
     8761 caattttata accaccggtt agtaaaatag caccaatttc gatgccattc attaccgcca
     8821 acgatgcagc aactaacact tcaggacgat ccgcagaggt aactaataaa ctaccggctt
     8881 taaaatgatc gaccatattt ggtaagttac gtgcacagaa tgtcacaccg cgaatccgac
     8941 gagcctttat ttcgccttca ttaataattg aagcgcctaa atgttttgca acatcaattg
     9001 ctcgtgtagc aataagttct gctttccagc taatacagcc taacagctta attggacttt
     9061 tagcaaaaag atgctcgact tctgaaacat tattatttga gtgctgaaat gaatcaaaaa
     9121 tttcagtcaa atctggacgg gttctacctg aatcatctac tggtgcattg aatttattga
     9181 taataacgcc cagtaaattt gggttgttac gtccaccaaa ttgtgaagcg gcagcttcaa
     9241 ctcgctcttt taattgagct ggtgtatctg aggccggtgc tgcaactaat acaatttcag
     9301 catcaagggc ttgagccatt tcatagttaa tgctgttagc gtatgagttt ttacgcgttg
     9361 gaattaaacc ttcaacaatg acaatttcat tattttttga taatttttgg tggcgttcca
     9421 ccactttttc taacaataca tccgtttggt tttgaccgat taatgtttca gcttcgctta
     9481 acataaacgg ctcaccaacc tctgttgttt gttctgctct gataatcgtt gttgagcggt
     9541 ctaacgtatc ttcaccgctg ataggttgag aaatcggttt taaaaatcct actttagaac
     9601 ctttttgctc caaagcatgt actaaaccta agctaacact ggttaaacca acaccagttg
     9661 aaatagggat aagaatgatc gttctagaca taatatttcc aaattaaaag aaagtgaaag
     9721 ggcagaatat ctgcccccta tgattataat gctaatttag cggtatcttg tgcaatcacg
     9781 agttcttcat tagttgggat aacaacagct ttaaatgttg actcgtccgc tgtaatgact
     9841 ccatctttac caaaacgggc agcaaggtta cgctcttgat ctaacttaat gccaaataat
     9901 tttaagtgat ttaaggttaa ttcacgtact aagcctgaat tttcaccgat accacctgta
     9961 aatgcgatag catctaagtg gtcatcgcct aaaattgcca tatatgaacc aatatattta
    10021 gctaaacgat agctatatac atctaaagca cgttttgctt ccggttttga tacgtcattg
    10081 taattatctt ccgcataacg acagtcacta gttacgccag ttaaacctaa aagaccagat
    10141 ttttttacta gagtagtatc aatttcgtcc attgacatac ctaagttatt gtataagaaa
    10201 gagataatcg cagggtctaa gtcaccagag cgagtaccca ttactaaacc ttctaatgga
    10261 gttaaaccca ttgaggtatc aatacattta ccattgcgta caacagaaac agaaccaccg
    10321 ttacctaagt ggcaaataat ggcattggtt tgctcaacag acttacctgc taattctgca
    10381 acttgtgagg tgatatagaa gtggcttgtg ccatggaaac cgtagcgacg aataccattt
    10441 tctttataga atgtatatgg aagtgcatat aagaatgcgt gttccggcat tgtctgatgg
    10501 aatgcagtgt caaatacagc tacgtttttg tcttttaatt cagggaaaat gcggaatgct
    10561 tctttaatac caattaaatg cgcagggttg tgtaatggtg caaattgcgt tgcatcttca
    10621 atacctttaa tcacttcatc ggtaattaca actgatgagg tgaatttttc accgccatga
    10681 acaatacgat gaccaatcgc gccgatacct gcttttaatt cgtcagataa caatttttca
    10741 gcaataaagg taagtgcttc actatgtgtg gcacctgcac ctaaatcagc attacctttc
    10801 tccccgttta acttccactt gatacgtgca tcttctaaat gaaaagcttc agcaagtcca
    10861 gataattttt catcacctgt tttaggatct aaaatagcaa atttaagaga tgaactaccg
    10921 cagttaagga ttaagattaa gtttttagac atggtcaaat taccttattt tatgaaatga
    10981 gtttgaagaa aatactattt ttattggtgt atttaaggtg taaaggatac acctttcagc
    11041 tcttaaaata aatgcttgaa ctcaaagaaa gataaaaatg tgtttaaaat ttgatataac
    11101 aagctataaa tgtgaaaaaa tatggcgaac aagcggttat ttttctaaaa tattttacaa
    11161 tttacccttt catttcatta aggagatatt ttatgttaaa tagaagaaaa tttatccagc
    11221 ttggtgcaac tgcaatgctt gtattgggga cacaaccgtc tgcatttgca caaacagcca
    11281 aaaataaagc tggcttgcgt gtcatcgcca ctacggatat tcacggtttc ttaactgatt
    11341 ttgattatta caaagatgct ccaacagaaa agtttggttt tacccgtacg gcaagcctaa
    11401 ttaaacaagc tagagcagaa gttaaaaaca gtgtgttagt cgataatggt gacttaattc
    11461 aaggtaatcc tatttctgac taccagtcaa ccaaaggtgc aaaagagggc aaacctgaac
    11521 cttcgattat ggcattaaat gcgatgcaat atgaagtcgg aacgctggga aatcatgaat
    11581 ttaactacgg tttaagctat ttagataatg ctattaagca agcaaaattc cctattgtca
    11641 atgcaaacat tgtgaagccg gggacagatg aaccattctt tacaccttat gttattcaac
    11701 aaaaagaaat tgtagatgaa aaagggaata agcataccat taagattggt tatatcggtt
    11761 ttgtaccacc acaagtgatg atttgggata aagctaatct tgaaggaaaa gttgaagctc
    11821 gagatattgt gaagaccgca caaaaatatg tgcctatagt aaaaaaagaa ggggcagata
    11881 ttgtcgttgc attggcacat accggccctt cagacgaacc ctataaagaa ggcgctgaga
    11941 actgtgcgtt ttatctagct gatgtaaaag gtattgatgc agttattttc gggcattcac
    12001 atcgcctgtt cccaaataaa gaatttgcaa atattgcaaa aggaacaatg aaaggcgtac
    12061 cggaaagtat ggctggctac tggggaaata acatcagtgt gattgattta agcttggccg
    12121 aaaacgatgg taaatggggc gttgtggacg gtagagcaga gttgcgtcca atttataata
    12181 cggaagagaa aaaagcaacc gttgagaacc accctgaaat cgttgcatta ttaaaagaaa
    12241 cccatgaagc aacccgtcaa tttgtgtctc aacctatcgg taaagcaact gataatatgt
    12301 atagttacct tgcgttagtt caagatgacc ctaccattca aattgtgaac caagctcaaa
    12361 aagcgtatgt agaaaatgtg gtaaaaggct tgccggagct tgcaggatta ccaattctaa
    12421 gtgccggagc accgtttaaa gccggtggac gtaaaaatga cccgacaggc tataccgaag
    12481 tcgataaagg cgaattaact ttccgcaatg cggcagactt atacctttac ccaaatacct
    12541 tagtggtggt taaagcaaca ggagcagaat taaaagagtg gttagagtgc agtgcaggaa
    12601 tgttcaaaca aattgatgtc gcttcggata aaccgcaata tttactggat tggacaggtt
    12661 tccgcaccta taactacgat gtaattgacg gtgtcagcta tcaatttgat ataacccaac
    12721 cggctcgtta tgacggcgag tgcaaattga tcaaccctaa ttcgcaccgt gtggtgaact
    12781 taacctttga cggcaaaccg gttgatccta aacaagaatt cttaattgcc accaacaatt
    12841 accgtgctta tggagcaaaa ttcccgggaa caggcgataa acatattgtg ttcgccgcac
    12901 cggacgaaaa ccgccaagtg ttagcgaatt atattaccac cgaaagtaaa gctcacgggc
    12961 aggtaagtcc gaaagcggat aacaactggc gtattgcacc aattagctca aatgcaaaac
    13021 tggatattcg ttttgaaacc tctccaacag ataaagctaa agctttcatt aaggaaaatg
    13081 cacaatatcc gatggagtat atcgaaaacg atgaaaccgg cttcgcagtt taccgtattg
    13141 atttaaccaa ataatcagag ctaaatacaa gcggtctaat ttgcaaaaaa attaacaaat
    13201 ttgaccgctt gtttcattcc atttttctaa aattgttgtg ctatattttg ccgaatttca
    13261 tcaaaattgg tcgatttgac caacttacca ttttcaaaaa ccacctctaa aagatcatct
    13321 gaaaaatcat ccagtgcggt tagctcgtca acataatgat cataagagat tacttttact
    13381 cgcccttttt gcgatttttt taagccgtta tcggttttag gagctttgaa aattgccttt
    13441 tcgacaccgt taattgtaat tgaagtcgct ttgattgcaa aacccagcgt atcacgagta
    13501 ttgcgttgat aagtttgagc cccaacgcca aacacaatat tgctggaagc aaaacctttt
    13561 gccgttaagc cctctaaaat ccgtatcatt ttatcataag tgacgccgtc accataaatt
    13621 gccccaatat gcggattaag cactttataa cctttttggt taatactacc accaaaaata
    13681 tcccacaaac actcaatcag acctttgcgt tcgtgttcaa caggggagga actgttaccg
    13741 caaataattg taaaaaaatc accgctatca gggcggataa ccaattttgc tgattccggt
    13801 cgagctaaaa tttcttgttt taataatggc aaattaacgg tgatgttatg ccagaaatcg
    13861 gttgtatctg ccacaataga tagcatatta tttggatatt ttgccattaa ataacggaaa
    13921 gtcggcagct catcaacacc gtgtgaactc atcaccgaat gttctgatgc cggaatagat
    13981 gtaccgatta aatgttgaga accataatag gctgtcacga atgaaattgc aggaatggta
    14041 tctgtgccta aaaatgaggt tagatgcccg gcaccggagc tttctgctga ttctaaggaa
    14101 ctcattccac gcatagagaa atcgtgtgcc tggaagggta gatggctatt atcatcacag
    14161 gtttgctctg aaaattcggc tagagcctta cgataagcaa atgcaatgga agctgatgta
    14221 ataggctgcc ataatgacac attaatcagg gtttccaaat aattagtcag ccaaaaaaag
    14281 cttgagtgag tgttttcaat tgtcattaaa ggaactttca ctgcaacaga ttttccttct
    14341 ggaatagctt taattctgat aggtaagtaa ccgagctgat gcaattgtgc aatatgttcg
    14401 ccagagtcct caatattcaa tgtgtcagaa ataaattgag tatattcaga aacaacgtca
    14461 tgttgtgagc gagaaaaaaa attatcgtta aaataatgaa tcaagtattt taaaataaat
    14521 gcctgaaagc caaatgatac aacacgtgta acaaatggag cataaatatt actacgaggt
    14581 gtgaatgtac tatataaaaa ctgagaacct tcaggatatt gaattcgatg tgaagtttta
    14641 taaaaatcac ataatagtga tggtattgca gttgaattaa tttttttacc caaaatacta
    14701 tccattttct taaagtcctt atttaacttt taaggctaaa ggttatcata acttaccata
    14761 aaatgaaaag tatttgttac aaaatgagtg tatttacata ataacttata gattaatgct
    14821 gttacataga tgtgatggcg tattcaataa taccgccgat tgatgaaaaa cttatattat
    14881 taattttcgc ataattatta ttatgttaaa taggtggtta aaataacaca tttaattaaa
    14941 tcttttcgtg tttctctcct cctttattaa aaaagaaaac ggtcagattg gtaaaatcta
    15001 tacctaatat gaccgcttgt gaatacaaaa aaattacttc aactgtttaa aggcttgcag
    15061 taaatcagct ttaatatctt caacgtgttc taaaccaacg gaaaaacgca gtaaattatt
    15121 cgtaatgcct cgtgtaatac gttctgcttc cggaatatcc atatgggttt gtgttgcagg
    15181 ataagtaata aaactttctg caccacctaa actttccgca aaggtaatca atttaattgc
    15241 cttgagaaat ttcgctaccc actcttcttt ttgtaaacgg aaagataaca taccactttt
    15301 gcctgaatat aacacgctgt caatttcagg ttgctctgct aagaatttag ccagttcggt
    15361 ggcattagac tgatggcgtt ccattcgtaa tgccaaggtt ttcataccac gcacggcaag
    15421 gtaagaatca aatggcgaca gcactgcacc ggcacagttt tggaaataga ataaacgctc
    15481 ggtcaaattc tgcccgttta ctttggtagc atcagaatct ttcgcaacaa ttaagccggc
    15541 taacacatca ttatgccccg aaagatactt ggtcgcactg tgaatcacaa tatcagctcc
    15601 gtgctcaatc ggacggaata acgctggggt taagaacgta ttatcaacga tcagcattaa
    15661 attgtgtttt ttcgcaattt tagcaatgac agcaacgtcg cactcctcca tcaatggatt
    15721 tgacggcgtt tccacgaaaa tcgccttagt attcggtgta atagctgctt caatcgctgc
    15781 taaatccgct gtattaacat aaactggctt taatgtgccg tgatttttgt ggctaaaatc
    15841 aaacaagcgg taagaaccac cataaacatc gctggaaata atccattcat caggtgcttt
    15901 aaataatgac attactagct gaatcgctgc cattccagat gccatcgcaa aaccggcatc
    15961 accgccttct aaatccgcaa tcccctgctc taatacagct cgagttgggt ttttggtacg
    16021 ggtgtaatcc cagccggtag attcgcctaa gccatcacgc ccgtaagcag ttgataagat
    16081 aatcggcatt gccaccgccc ctgtacgagg atcagttctg ttaccgattt gcactaatgt
    16141 ggtttcaata ttattgaatt ttgtcattgt tattatcctt caattacaga ttatttagat
    16201 ccaatacgtc tgtcatatca aacaagccat tttgtttgcc atttaaccat tttgcagcac
    16261 gcactgcgcc attggcaaac gtcattcggc tagaggcttt atgtgcgatt tcgacacgtt
    16321 cgccttcatc agcgaaccat acactatgct cccctacaac atcaccggca cgaatagtag
    16381 caaagccgat ttcatcatgt ttgcgttcgc ctgtaatacc gttacgttca aatacgccat
    16441 gagttttcaa gtcacgccca agagttttgg caatatgttc tcccattgat aaagccgtac
    16501 ctgatggtgc atcaacttta tggcgatggt gtgcctcaat cacttcaata tcacaataat
    16561 cgcccattac ttttgctgct ttttcgagca gtttaaacac cagattcacc ccaacgctgt
    16621 agtttgaggc aaaaacgata ccgatttttt cactcgctgc tttgattgca gctttacctg
    16681 catcatcaaa accggttgtg ccaatcacca tttgtttatg atgagccaca caaatatcca
    16741 aatgttctag cgtgccttca gggcgagtaa aatcaattag taaatcaaaa cctgctacat
    16801 tttgggcgac atcatcagaa atcaccacac ctaatgtacc aaccccggct acttcaccag
    16861 tatcactacc tactaatgat gaacctttac gctcaaaagc agaccctaat tctacgcctt
    16921 gtgcattatg gacggcttga attaattgac gccccattcg cccaccggca ccgacgattc
    16981 caatttttaa tgtcatattt gttcttcctt tttatatttg ttttttacaa gcggttacat
    17041 ttttaattaa ttttgcaaat agctttctga tggcaattta aaaggtgggt gagaagtaaa
    17101 atcaacaatt tgaataaagt tttcaccttt cacataatgt aaaatatact cagaagtata
    17161 attaccaaat ccttgactgg tatcatattc tggaatgccg atatgataat gccataaaga
    17221 atatttttgt gctttagcca ccttctctgc ccaataagga tcattcgttg gcacatcatc
    17281 agaactttta tttcgaccag ctagattttc caatccataa atttcaacgt gttgtttaaa
    17341 ctgcaggatt ttgactagat ctttttcagg caaatattgc aattcggctg aaatttttgt
    17401 accaaataaa actttaatgc ccatttgcaa cacctttcaa ccagtttcta aattcattaa
    17461 cattcgttat atctttaggc acttcgtgaa aagtatcatt taaccgctct ttcattcgct
    17521 ctaagtcaaa attaaatgcg gttgcttgag agagttgctg ttcaattttg cgaatattgg
    17581 acttcatatt atccaatata gccattgtcc ttgagccatc agtaaaatac tgttgggtat
    17641 cgaaaatcac caccttattt tgatttaggt attttttgaa atcgtttaaa tcaatttcag
    17701 cccaaaagtt tggatttttt tcgatagttt caatacaggc aattaataat tgatttgcca
    17761 ttgtaatacg aaaatttaac aatgagattt gctgctctgt tgcaggttta tcatcagctg
    17821 cgaaatcaaa tgccggataa atcactgcaa gcgtgtcagc catagataag ggtaaatttt
    17881 tcatcatctc tccttacgga ataagttgtc attatctact ggattactta gaaaaacaac
    17941 ttattcattt tatgctattt ttgccccatc accgcaatat gtaccgtaat ttcctcacgg
    18001 tcgtggtaga ggtgtttagc ttgcattttg aaccaaaacc cgtgtttcgt cagctcttct
    18061 tccaagaaat tgaggcatag ttgcacttct tgataacgtt ttttcatcgg cagttttagg
    18121 ttgaaaatcg tttctcgaca ccaaccgtta gtcagccatt ttgcaatgag tttcgagata
    18181 cgaatcggct gctctaccat atcacaaacc agccaatcga tttgtttacg cttaggcggt
    18241 tggaatttaa acccatcttc agggcaatgc tcaattcttc cggtttcgtg taaacttgct
    18301 gccattttgc cgtgatcgac tgcatagaca aaaacaccac gtttgactaa ttgataagtc
    18361 caaccacccg gacaagcccc aagatcgacc cctaccatcg tatcattaaa gcgttttttc
    18421 tcctctgctt gcggaataaa agttaaaatc gcctcttcta atttaagcgt tgagcgacta
    18481 ggtgcctcag ccgggaattt taaacgggga atccccataa aaaacggcga tttattatgg
    18541 ttgtacgcat agcccacata gcaagctccc ggacgagtaa ataaaatatg caagctcaca
    18601 ctaccctgca tattttccga tttccccaac caaccttgtt ttttcaatgc cgaccgtaat
    18661 ggcacagtaa atttacgaca gaaagttaat aattctttcg cttcgttggt atccggtgtt
    18721 tcgacaaata catcagaact ttggcgagga ttaagttcgg catattgggc taaaatcggc
    18781 gaaattcgat cattttcaga cagattttgc aacaaatccc caacaaccaa catttggcga
    18841 acaaaaataa gctgttcaaa ctttaacgct tgggctaatc gatctgcttc ccctgcttga
    18901 tagcactcaa aaatcacata gccactattt tctttaaggt tggcaaagcc aaaaatgccc
    18961 tgttgtgcag ctttatccgt gatttcgcct gctacctctt tttcaaaccc tgctcggcaa
    19021 tataaggcta atttattgaa cattaggctt ttctcacact gatataaatt aacgcaatcc
    19081 aaccaagcat aaagcagaga ccaccaatag gcgtaagcca atgaatagtc ggcacattta
    19141 acgccagtaa gtacaggttg ccactaaaaa gcagaatgcc taacgcccaa ttgcttccga
    19201 tgatgttaaa ggctttggct ctacaaacgg gtggattttg cccaaaattt gcaatttgga
    19261 ataaacctaa agctaaaatg gcaagggtgt gaaacatttg atatttaatc cccgtctcaa
    19321 tccagcctaa ggctttaggc gataattgag ccgataatgc gtggctggct atcgccccaa
    19381 atgccacagt aaataagccg ctgaatgcgg caattgctac aaatttgttt ttcataagtt
    19441 gaagataggt atgatgttag atggcttact atatcatatt atcgaaacca acaagcggtg
    19501 aaattttgca aaaaaattac aaaaaatcac cgcttgtatg cttaatggtt caagataata
    19561 tccacatctt ccgatttatc gtgaacccca aatctatcta ctaaggtttt gctggcctca
    19621 tttaatccga taatttctac ctcaacatca ctttttcgat atttcaacac aactttatct
    19681 aacgctgaaa ctgccgttac gtcccaaaag tgagcgtggg ttaaatcaat gtggactttt
    19741 ttcagttctg aacgggtaaa atcaaattgt gcgataaaat catcagatga ggcaaaaaat
    19801 acttgcccga taacatcata acgcaattcc tctttctcta tagcacttgt tttaaccgcc
    19861 atataacgtc caattttatt cgcaaagaat aatgccgaga gtaatacgcc ggaaaatacg
    19921 ccaagggcta agttatgtgt ataaaccaca atcgcaacgg tgactaacat cactacatta
    19981 aagccgattg ggtgcgtttt tagctcttct aaggattgcc aattaaaggt gccgatagag
    20041 accataatca tcaccgcaac taatgcagcc atcggaatca ctttcagaat atcgctcaaa
    20101 aataccacaa aaatcagtaa caaaaaaccg gcgagaaagg tagatagtct tgtacgtcca
    20161 ccggatttaa cattaatgac cgactgccca atcatcgcac aacctgccat accaccaaat
    20221 aagccactaa taatattggc aatgccctgc cctttacatt cacggttttt atcgctgtct
    20281 gtctctgtca tttcatcaac aatcgttgcc gtcattaaag attccaataa ccctactgcg
    20341 gccaagctga aagaataagg caaaataatc cataaggtgt ctaaattaag cggaatatcg
    20401 ggtaataaaa acaccggtaa ggtatcgggt aattgcccca tatccccaac ggtacgaaca
    20461 tcaatgcctt gccataatgt gaaaagtgtt aaagcaataa tggtcactaa tggtgaggga
    20521 atgatttttc caatcaccgg tacataagga aaaagataaa tgatagcaag ccctgctacc
    20581 accagtgcat aagtatgcca agttgtattt tgtaactcag gtagttgtgc cataaaaatt
    20641 aaaatggcaa gtgcattaac aaacccaatc actacgtctc gtgccacaaa ccgcataagc
    20701 gagccgagtt ttaaccaacc ggcaataatt tgcaacacgc ctgttaataa ggtggccgct
    20761 agcatatatt gtaaaccgtg atccctcact aagcctgcta ataccagagc cattgcaccg
    20821 gttgctgcag aaatcatcgc cggtctgccg cccacaaaac tgataattac tgcaatacaa
    20881 aatgaggcat ataaaccaat ttgtggatca acccctgcaa taattgaaaa tgctatggct
    20941 tctggaatta acgccaaagc caccactaaa ccagagagaa catcacctcg tacatttgaa
    21001 aaccattgtt gtttaattac cgtaggcgaa aattgctgaa ataaagttgt cattattacc
    21061 cttaaataaa aagaaaagcc gagcaaagtc ggctaaaaat gaggcgagag agtagcaaat
    21121 tagtgaaaag tcgtcaaata aacaagcggt cttctttcgc tctttttttg caaaaaatag
    21181 tagaaaaatg accgcttgga ttaattgcta ttcactaaaa aaaggggctt taaacaccgt
    21241 cacttcaggt gcatctccca catatttttc cacttgaacc gtggtagtgt tcggtgcatt
    21301 gccctgcccg agttttgatg aaccctcatt acgggttagc acattcgggt tgccgttttt
    21361 gcatagtgga gtatctgttt tgcctaaatc ttgcgggtca taccaaccac cttcgtataa
    21421 cgctactgtg ccttgaatga tgccatcagt caccactgct cctgccaaca cctgccctcg
    21481 cttgttaaaa attcgcacaa tatcaccgct tgtaatgcct ctgtcagcgg catcttttgt
    21541 gtgaattagc attggttctc tgccgtttac ctcgtaatat tgacgtaatg aggagtaagc
    21601 aagctggctg tgtaaacggt atttaatatg cggtgtaact aatgccaacg gctcggcttc
    21661 gttggttttg cttgaatatt cgtgatgttc aaaccaagtc gggtgtcctt tacaatcttc
    21721 ataattcatc ttggcaatgg tttcggagaa aatctcaatt ttgcctgatg gcgttgccaa
    21781 tttgtgttta atcggatcag tacgataatc ttgatagcga acgaaagtat tattttgatg
    21841 ttctgcctca aagctcaaca ctttattttc cgcccaaaat gcctcgaaat ccagcaatgg
    21901 cgtattagct ttttcccccg cctcaaaggc aacttggtaa aattccttaa gccattccat
    21961 ttcgcttttg ccttcggtaa atgcttgttc tttaccggct cgtttcgcta attcggcaaa
    22021 aatatcgtaa tcattcttcg cctcaaattg cggttcaacc acctgtttca tcgggataat
    22081 atgttttgct acataatcac cggcaacggt gagatcgttt cgctcgtagc tggtggttgc
    22141 gggcaataca atatctgcca ttcgagcggt cggtgtccag taacattcat tgacgatcac
    22201 cgtttccggc tgttgccacg ctttcactaa atagttggta tccgggtggt gagcaaacgg
    22261 attacccccc gcccaataga ctaatttgat gttcgggtaa tgtaatgttt gcccgttgta
    22321 atcataaggc tgattcggtt tgagcaacgc atcggcaatt cgagacaccg ggaaaaacgg
    22381 catatcgggg ttaggtttac ctgcattgat tgcccctaaa cgaacaccgg aattaacatt
    22441 tagcccattg gtagaataac gataatttaa gccgaaaccg ccgcccggca agccaatttg
    22501 cccgagcata gcggcaagag tcactaacgc ccagtggctt tgctccccgt gttgcaggcg
    22561 ttgcaagccc cagccggaca tcaacattgt gcggtgagta acaaaatcat ttgctaaggt
    22621 tttaatcacc tcctccggaa tgccacaaat ttggctcgcc cactctgctg ttttaggtaa
    22681 attatctgct tttcctaaca cataatcagc aagttgctcg aaaccaacag tatatttagt
    22741 taagaagtcg tgatcgtgct tgttttcagt aataagggtg tgggcgatcc ctaaaatcag
    22801 tgcaacatcc gtgccggtat taataggcaa ccactctgcc tctaaaaact gacaggtctc
    22861 gcttttcacc gggtcgatac aaataatccg tttaccgctt gcttttaatt tggcgagata
    22921 atcaatcccc tccggttcgg cagaggtcca cgcaatgcga agtgtattca gtggattaga
    22981 cgaccaaatt accaataatt ctgtgctttc tacaatcgtg tgccaactgg tttgttgagc
    23041 atacacctca atattgccta atacataagg cataatcact tgcaccgcac cggtggaata
    23101 atcgccttta gtattggtaa agccgcctgt gacgtttaaa taacgttgca aaagcgttcg
    23161 agatgcgtgc agtgaaccac agctatacca gccgtaagag ccgccgaaaa tggattcgct
    23221 gccatattgc tctcttaccc gttggatttc gccggaaacc agctcaaagg ctttgtccca
    23281 agagattctc acccacttat ctcgccctcg caaagtggtg tctttattcc cttccaagta
    23341 gcctttccgc accatcggat atttcacccg aaccgggcta tagagctgat ccgccaccac
    23401 atcttgcaat tcgttttggt gatttttcgc taaactgccc tctgatttca ccacttttcc
    23461 gttttccacc gttacattta atgcacccca gtgggcggcg gtggtaatgg tatttttcgg
    23521 tttatccaaa aagacaaact tgcctttttc taatgctgtg ttcatttaaa ttaatgcctt
    23581 ttctgttata aaattgagag gaatttactg gattaagcca aaagttgcaa ccacaagcgg
    23641 ttgtttttag ccaaaaattt gcaaaacaaa acccacctaa aaaggtgggc ttcaaggaag
    23701 ttaaacggca acttgaacga ttacattgtc cgcttttttc acataactat tgatttggtc
    23761 aaagttcata tagcggtaaa tatcgtcctt atctttctgc aagttattca tatagtctag
    23821 gtactcttgc ggtgtcggca atttacccaa taacgcagaa actgcggcta attctgctga
    23881 ggctaaatac acatttgccc cttgccctaa acggttcggg aagtttcggg tagaggtaga
    23941 aaccacatta gcattatttg ccactcgggc ttggttaccc atacagagag aacagcccgg
    24001 catctctacc cttgctccgc ttttgccgta aatgctgtaa tagccctctt cggtcagcaa
    24061 gcctgcgtcc attttagtcg gcggagcaat ccataattta gtcggcaatt cgccctgtgc
    24121 ttggcttaac agtttacctg ccgcacggaa atgcccgata ttagtcatac aagaaccaat
    24181 aaagacttca tcaattttat cgccctgtac ttgagaaagc aggcgagcat cgtcagggtc
    24241 attcggtacg cacacaatcg gctctttgat ttcatctaag ttgatctcaa tcactgcggc
    24301 atactccgca ttttcgtctg cctctaaaag ctccggatta tccaaccacg cttgcatatt
    24361 ggcgatacgg cgttctagcg tgcgtttatc gtcatagcct tccgcaatca tccattttaa
    24421 aagcgtaata ttagaattca gatactcgat aatcggctct ttgttgagct tgatggtaca
    24481 ggctgcagca gagcgttcag cagaagcatc agaaagctcg aaggcttgtt cgattttcag
    24541 atcttccaac ccttcaattt ctaaaattcg accggagaaa atatttttct tacctttttt
    24601 ctccaccgtc agcaagtctt gctgaatggc ataatacgga atagcgtgaa ccagatcccg
    24661 taaggtaatg cccggttgca ttgtgccgga aaaacgcacc aagaccgatt ccggcatatc
    24721 taacggcatc acccctgttg ccgcagcaaa ggcaactaag cccgaaccgg cagggaatga
    24781 aatcccgatt gggaaacggg tgtgggaatc gccgcctgta ccgacagtat ccggcaataa
    24841 catacggttt agccaagagt gaatcacgcc atcacccggg cgaagtgaca caccgccacg
    24901 gttcataata aaatcaggca aagtgtggtg ggtaattacg tccaccggtt tcgggtaagc
    24961 ggcagtatgg cagaacgatt gcatcacaag atctgccgag aagcctaaac aggctaaatc
    25021 tttcagctca tctcttgtca ttggtcctgt ggtatcttgt gaaccgacag acgtcattcg
    25081 aggctcgcaa tattgccccg gtcgaacgcc ttctacgcca caagctcgcc ctaccatttt
    25141 ttgagcaagg gtgtagcctt tgttactctc tgaaaccgac tgcggtttaa taaatacatc
    25201 actttcaggc aagccaagtg ctactctggc tttatgggtc aaaccacgtc cgataatcag
    25261 aggaattcgc ccgcctgcac gcacttcatc aagcaatact tcggttttta gctcaaattc
    25321 ggctaagact tcatcggagt tgtgtttgca aattttgctt gcataagggt aaatgtcgat
    25381 tacatcgccc atattcaggt tggttacatc cacttcgatc ggtaaagaac cggcatcttc
    25441 taatgtgtta aagaaaatcg gggcaatttt accgcctaac accaccccac cggcacgttt
    25501 gtttggaatg taaggaatat cttcccccat aaaccaaagc acggagttgg ttgcggattt
    25561 acgagaagag ccggtgccga ctacatcacc gacataggct aacgggaaac ctttttcttt
    25621 taatgcttcc agttgcttaa tcgggccggt atgaccgttt tcatcgggga aaataccgtc
    25681 acgttcattt ttcagcattg ctaatgcgtg aagtggaata tcagggcgag accaagcgtc
    25741 ttgagccggg gaaagatcat cggtattggt ttcgcctgtg actttaaata ccgttaccgt
    25801 taatttttcg gcaagtttcg ggcgagaggt aaaccaatcg gcattcgccc aagattccaa
    25861 tacttgttgt gcaaacggat taccctgctc tgcccgttct tttacatcgt ggaaattatc
    25921 aaacatcaat aatgttttag ataaggcttc taccgcaatc ggtgcaagct cagggttatc
    25981 caacgcagac aataaggttt caatgttata accaccttgc attgtgccga gtaatttcac
    26041 tgcgtgagca ggcgaaatga gcggcgatgt ggcaacccct ttggtaatat cggctaaaaa
    26101 ggcggcttta acataagcgg cttcatcaac acctgccgga atacgattgg taaaaagatc
    26161 caataaaaag accgcttgtt cagccggtgg gtttttcaat aattcaacca gttgagccgt
    26221 ttgttccgca gaaagtggct gaggcacaac acccagtgcc gcacgttcat caacctgttg
    26281 ttgataattt tctataaaat tttccatcat cattccctct ccaactttac atattggcaa
    26341 taatgtcttg tgcaaattct gaggttgagc gtagagttac cccctccagc atttccgcaa
    26401 aatcaaaggt gacagtttta ttggcaatgg ttttagacac cgcttttacc actaaatcag
    26461 ccgcttcaac ccaacctaaa tggcgtaaca tcatttcccc acttaaaatt aatgacccag
    26521 gattgccttt attttgccct gcaattttag gggctgtgcc gtgtgtggct tcaaaaatag
    26581 ccgcttcaaa cccaatattc gctcccggtg aaataccgat tccgcctact tgtgcggcta
    26641 aagcatcaga aatatagtcg ccgtttaggt tcagtgtagc aatcacatca tattcggttg
    26701 ggtgtaacaa gatttcttgt aaaaaggcat cggcaatgct gtcttttata atgatttcct
    26761 caccggtttt cggattagtc aagctcatcc aagggccttt atcaattaat tttgccccaa
    26821 attcttccgc aacttggtag ccccactctt taaatgctcc ttcggtaaat ttcataatgt
    26881 tacctttatg cacaagcgtg agcgatttac ggttgttatc aattacatat tgcaatgcag
    26941 cacgcaccaa gcgttgtgtg ccttgcttag aaaccggctt aattccgata ccgcaatctt
    27001 cggtaaagcg gatttttttc acgcccatct cttgttgtaa aaattcgatc actttattgg
    27061 cttcaggcga accggcaacc cattctacac cggcataaat atcttccgag ttttcacgga
    27121 aaatcaccat atccaccagc tcagggtgtt taaccgggct tggcgtaccg tcataataac
    27181 gaattgggcg taagcagttg tataaatcta atccttgacg catcgccaca tttaaagaac
    27241 gaataccgcc acccaccggt gtcattaaag gacctttaat cgccacgtga tattggcgga
    27301 taagctctaa ggtttcatcc ggcaaccaag tgttctcgcc atataattta ttcgcctttt
    27361 cgcctgcgta aatttccatc caagcaattt ttcgcttgcc ttgataggct ttttctaccg
    27421 ccgcatctag cactgctttc attgcaggag taacatcaac accgatgccg tcaccttcaa
    27481 taaatggaat aatcggatta tccggaactt gtaaagaacc gtcttggttc agttgaatag
    27541 tttcgccttg tggaatttgg acttgtgttt gcattgattt tacccccgta atgacgaaaa
    27601 agtagtgacg aaaaatgaat gttgacaatc agccaacaat ggattaacta taaacagtaa
    27661 atttctaaat gtaaataaag caaaatgggc tgagagagaa tttgtgatct aacgcacatt
    27721 tatgtaaata aaatgtaaaa aatcgttgtg attcttttgg aaatcatcta gtttatctta
    27781 taaatctcaa ataaatagga gtaagctatg tcaaaagcaa cattaacact cgaaacaggt
    27841 gaaaaacttg aactcaatat gcaaaaagga accttagggt atcaaacgat tgatattcag
    27901 cctttcttaa agcataagtt atttgcttat gatcccggat tggtttcgac cgctgtatgc
    27961 caatccaata tcacttatgt tgatggcgat gaaggggttt tactctatcg aggctacccg
    28021 attgaccaat tagccaaaca ttctgattac ttagaagtcg gttatatgct gttattcggc
    28081 gaacgcccta ccaaagaaga atacaaagac ttcgttcaac tcatcaaaaa acacactctt
    28141 gtacacgaac aactaaccaa attcttctcc ggtttccgcc gagattcgca cccaatggcg
    28201 gtgatgtgtg gggtaagtgg agctttggct gccttctacc acgacgccat tgatgtaaac
    28261 aaccaagccc accgtgaatt aaccgccatt cgtttgttag ccaaaatccc aacccttgcg
    28321 gcgatgtgtt ataaatactc tatcggtcag ccgtttatgt tcccacaaaa ccacttatct
    28381 tacgccggaa acttcttata tatgatgttt gccactcctt gtgaaccgta cgaagtgaac
    28441 cctgtgttag agcgtgcgtt agaccgaatt ttcattttac atgcagacca cgaacaaaac
    28501 gcttcgacct caaccgtgcg aaccgccgct tcatcaggtg caaacccatt tgcttgcatt
    28561 gctgccggta ttgcctctct ctggggacct gcacacggag gagcaaatga agcctgtatc
    28621 aatatgttgg aagaaatcgg cactgtggat cgtattcctg aatttattgc tcgagccaaa
    28681 gataaaaatg acccgttccg cttaatgggc tttgggcatc gtgtctataa aaactacgac
    28741 cctcgtgcga aagtaatgcg tgaaacctgc cacgaagtgt taaaagaact gaacattgat
    28801 aacccactat ttgaagtggc attggaatta gagcgtattg cgttaagcga cccttatttc
    28861 attgagcata aactttatcc aaacgtagat ttctactccg gaatcgtgct aaaagccatc
    28921 ggtattccaa cctcaatgtt taccgtaatg tttgccctag ccagaaccgt tggctggatt
    28981 gctcactgga aagagatgta tctccaaggc aatatcaaaa tcgttcgtcc acgtcaaatc
    29041 tacaccggac atactaaacg tgattttgag ccaatggata aatcataaga ttagcaaaaa
    29101 atcgaaaaat tttaaccgct tgtggaaaca agcggttata tttttcataa aatttgcaaa
    29161 tcaggctaat ttatttagca gttgtgttct aacatcttga ccattttcat cgctttttac
    29221 agccataata aataagcctt ttccttgact taatttctcc caaagattgc caattaaggc
    29281 tttttcttta gaatcttcat tactttttaa ttgttcccct ttatattcca caattaaaat
    29341 tcgaccatca ataagctcta caataaaatc agggtaaaaa ttctgat
//
LOCUS       DANKBV010000013        29268 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.013, whole genome shotgun sequence.
ACCESSION   DANKBV010000013 DANKBV010000000
VERSION     DANKBV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 29268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29268
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.013"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..213
                     /locus_tag="PWY43_000470"
     CDS             <1..213
                     /locus_tag="PWY43_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL2135379.1"
                     /translation="GSKTASRLAFNRGLITQGEVWLEMIKSRNITVHTDDETILTEEF
                     NKIIKAYLPLFIEFEQRVETLCQTLD"
     gene            195..506
                     /locus_tag="PWY43_000471"
     CDS             195..506
                     /locus_tag="PWY43_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2135380.1"
                     /translation="MPNFGLSERATKTITDILAKYPEVEQAIIYGSRAKGNYREGSDV
                     DLTFKGKDLTYDIVKRIWGDLDDSDSPYLFDLSIYHQLTNANFIEHIDRVGKVFYQRE
                     L"
     gene            617..1780
                     /gene="mnmA"
                     /locus_tag="PWY43_000472"
     CDS             617..1780
                     /gene="mnmA"
                     /locus_tag="PWY43_000472"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL2135381.1"
                     /translation="MTTQLTSKTYDTHFPKLTPEQLAENSKKKVIIGMSGGVDSSVSA
                     FILQQQGYQVEGLFMKNWEEDDDTDYCTAAADLADAQAVADKLGMKLHKINFAAEYWD
                     NVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEYAAEDLGADYIATGHYVRRTSDDQD
                     AKLLRGLDENKDQSYFLYALNKKQIGQSLFPVGEIEKPIVRVIAEDLGLATAKKKDST
                     GICFIGERKFKDFLARFLPAQPGEIRTVEGKLVGRHDGLMYHTLGQRKGLGIGGVKGL
                     SEDPFYVVEKDLINNVLIVAQGHDNSALLSSGLIAKQLTWVDMQPLRENLRCTVKTRY
                     RQADIACEIQVLDDETIRVMFDEPQIAVTPGQSAVFYLGEVCLGGGIIESQLK"
     gene            1897..3276
                     /gene="argH"
                     /locus_tag="PWY43_000473"
     CDS             1897..3276
                     /gene="argH"
                     /locus_tag="PWY43_000473"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDL2135382.1"
                     /translation="MALWGGRFTQQADAKFKYFNNSLRFDYRLAIQDIEGSIGWAKAI
                     TSVGILTVAELEQLIAALKEVRAEVESNLTIILKDDAEDIHSWVESKLIEKVGDLGKK
                     LHTGRSRNDQVAVDIKMWCKVQAVALQERIRALQERLVSVAEENQESVMPGYTHLQRA
                     QPVTFAHWCMAYYEMLERDFSRLSDANKRMSTCPLGSGALAGTAYGIDRDLLARDLGF
                     EEATRNSLDSVSDRDHILELLSTASISMMHLSRFAEDLIIFNSGESGFLEMSDRVTSG
                     SSLMPQKKNPDACELIRGKSGRVFGALNGLLTTLKGLPLAYNKDMQEDKEGIFDALET
                     WQACLEIAELVLVDIQVNTERTREAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VYAISKKEPLEALSINEFQQFHHSIGDDVYPILSLESCLEKRCAKGGVNPQRVAEAIA
                     VAKANLASK"
     gene            3444..4442
                     /locus_tag="PWY43_000474"
     CDS             3444..4442
                     /locus_tag="PWY43_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HDL2135383.1"
                     /translation="MTESVNSTQAGTALSLGQQLKAAREALNLSITDVAQKTNLKKSH
                     IESLENDIFILQNVAPTFVRGYVRNYVKFLRLPESLISSVNYGEVTIPKEVTKVSSVK
                     PSSNSQGKTLKYLTVLVLLAALGMTLLWWWQGYQKEQENREQFVSSAPVAEVANTATN
                     NDASAQTSVPVQQPIQVSEQPAQVAETVVAAEPALTEKVEPTQAASSPVTQEDVKVVE
                     EVKQADATTAQVEAVNVLQQSQSEQAPTEQPVAVTNDELRIEITGSQSWITVRGAKNK
                     RLAEKLYNNGETLSFNENEQYRLTIGAPANVKVYYKGQEVPLKIDGRVARIRLPLQ"
     gene            4545..5657
                     /gene="ispG"
                     /locus_tag="PWY43_000475"
                     /gene_synonym="gcpE"
     CDS             4545..5657
                     /gene="ispG"
                     /locus_tag="PWY43_000475"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDL2135384.1"
                     /translation="MIHQSPIKRRVSKKIYVGNVPVGGDAPITVQSMTNTRTTDVEAT
                     VAQIKSLERVGADIVRVSVPTMDAAEAFKAIKQQVSVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRTVVDCARDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLES
                     ALRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARAGSV
                     KSAVGLGLLLAEGIGDTLRVSLAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EIDVIATVNELEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGVTGSNKMSGFYLDGV
                     RQKERFDNAKLIDQLEAKIRARVAQQHNRIDIEQIG"
     gene            5663..6940
                     /gene="hisS"
                     /locus_tag="PWY43_000476"
     CDS             5663..6940
                     /gene="hisS"
                     /locus_tag="PWY43_000476"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL2135385.1"
                     /translation="MAKTIQAIRGMNDCSPSESPLWQWVEQKVRNVLASYGYSEVRMP
                     IVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQ
                     RLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIANPEIDAELIILTARLWKELGIDQHV
                     SLQLNSIGSLEARANYRSALVKFLENHTALMNEEEKERLVKNPLRILDTKNEALQNVL
                     NDAPKLLDYLDDDSREHFTQLCALLDAMGIRYEVNPKLVRGLDYYNKTVFEWVTSALA
                     LGSQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEIDLPKTVDVYI
                     VFSGEGATLNAFQLAEQIRSELPHVGVMTHCSGGKFQKQFKRADKVEAQIALVIGESE
                     VAEKQVVIKDLRSGAEQITIAQSELIAELSKRF"
     gene            6950..7570
                     /locus_tag="PWY43_000477"
     CDS             6950..7570
                     /locus_tag="PWY43_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2135386.1"
                     /translation="MSNEYLNKTEEQQFEDAKNWFKENGTPILVAIILACAATAGWNF
                     WKNHQLETAQKTSASYQSVMESYLQNPSKNQPLVEKFIADNKDSSYAAFAQFEVAKQA
                     VEKGNFSAAKTALQSVLTYSDSTLQSIARFRLAQIDFQLKSYDEALASLAQIQDKAWE
                     LRKQILTGDILAAKGDIPAAKSAYEQAKTTANPQEQTLIDVRLNNL"
     gene            complement(7633..8736)
                     /gene="dnaN"
                     /locus_tag="PWY43_000478"
     CDS             complement(7633..8736)
                     /gene="dnaN"
                     /locus_tag="PWY43_000478"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL2135387.1"
                     /translation="MQITITREQLLKPLQQVCGVLSSRPTLPVINNLLLEIEGNRLSL
                     TGTDLEVELTTQAQLLQAVDFAGKFTIPAKKFLDISKSLPDDSVISVQFEEDKAIIKA
                     GRSKFSLATLPASDYPNLMDWKPEVDFSIDQSTLARLIDATQFSMANQDARYFLNGMK
                     FETEGNLLRTVATDGHRLAVCTMVLNQELLTHSVIVPRKAVLELSRLVASNDETVRLE
                     IGSSNIRITMNGIVFTSKLIDGRFPDYRRVLPRNADRILEAETETLKRALMRAAILSN
                     EKFRGVRLTLSNNLLKITANNPEQEEAEEIIDVAYDSPEMEVGFNVSYVLDVLNTLKC
                     QRVRFSLVDSSSSCLIEDCDNSSAEYVIMPMRL"
     gene            complement(8755..10104)
                     /gene="dnaA"
                     /locus_tag="PWY43_000479"
     CDS             complement(8755..10104)
                     /gene="dnaA"
                     /locus_tag="PWY43_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL2135388.1"
                     /translation="MEQIVSSLWSDCLNHLQTKVSPTDYSTWLRPLQASSENGELTLY
                     AQNQFVENWVKDKFLSEITELARFLAKNDNLVVTLKVGIKPTEQPKVSPNLKNESSEF
                     SNTGFKTGLNPNQTFDNFVQGRSNQLAKLVAQQVVDNLGESHCNPLSFYAGTGLGKTH
                     LLHAIGNELVKRTPGTRVLYIHLERFYRDIIKAINSSNNDAEKIKKFYRSLDVLMIDD
                     IQFLANKPKVQEEFLHLLNSLFEQRKQIILTSDVLPKNIDKIDTAIKSRLSGGISATI
                     EPPELETRVAILRQKALERGADLQEDAAYLMGQKLRTNVRELEGALNRVIAWQKLTLR
                     PITVDAVRETLQDLFASFDYLITIENIQRIVAEYYGIKVADIKSKSRARSIARPRQMA
                     MALAKELTNHSLPEIGREFGGRDHTTVMHACKTINELRDTESGIQEDYIHLTRKLSS"
     gene            complement(10291..12138)
                     /locus_tag="PWY43_000480"
     CDS             complement(10291..12138)
                     /locus_tag="PWY43_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2135389.1"
                     /translation="MFEKLFQWFEARVETYPNETPRTPKSGLIPFIYDATKGMRFYIL
                     LLTVLVAAVGIIEAVLFRFMGDLVDWVNKYSPTELWAEKGWTIIGMFFIALLAVVFVY
                     LASSIRFQSLQGVFPMRLRWNFHRLMLGQSLGFYQDEFAGRVSAKVMQTALSVRDVIM
                     TCADMLVYVLVYFTTSSVILLQLDGWLFVPFILWVISLAATIIYFVPKLAQAAQEQSD
                     ARSLMTGRITDAYANIATVKLFSHGNRESAYAKESMEEFMVTVHKQMRLVTVIETVTN
                     LTSMALIISTAGIGLWLWGSSVVTAGAIATSTALALRIKGLSQWIMWEFARLFENLGT
                     VQDGMITLSKPHNVVDKIDAKPLNVTKGEIKFENVDFAYDPKKPLLKDFDLTIKPGEK
                     VGLVGRSGAGKSTLTNLLLRFYDIQDGSITIDGQNIRDITQESLRSQIGLVTQDTSLL
                     HRSVRENLMYGRPNATEEEMIKAVEQAAASEFIPNLQDAKGRTGFDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEVAIQENLTQLMEGKTVVAIAHRLST
                     IMAMDRLIVLDKGEIVEQGTHEELLAQNGVYARLWAHQSGGFLPENTDI"
     gene            complement(12206..12715)
                     /gene="luxS"
                     /locus_tag="PWY43_000481"
     CDS             complement(12206..12715)
                     /gene="luxS"
                     /locus_tag="PWY43_000481"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL2135390.1"
                     /translation="MPLLDSFKVDHTKMNAPAVRVAKTMTTPKGDTITVFDLRFCRPN
                     IDILPVRGIHTMEHLFAGFMRDHLNSEQVEIIDISPMGCRTGFYMSLIGTPSAEEVVK
                     AWTASMEDALDKIPDVSAIPELNEYQCGSYKEHSLEEAHQIARNVLAAGIGVNRNEDL
                     ALDEKLLNP"
     gene            complement(12733..15399)
                     /locus_tag="PWY43_000482"
     CDS             complement(12733..15399)
                     /locus_tag="PWY43_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HDL2135391.1"
                     /translation="MTMEENTHTVVDAQIRQPRKISPFWLLPIVAFIIGALLFFQILK
                     EQGETITIRFSKGDGITAGKTAIRYQGLQIGQVKRVYFVDNLKEVEVQAEINPEAKSI
                     LRKQTKFWLVQPSASLAGVSGLDAIVSGNYITLLPGEGDFVDEFIAEEDPPAITVSDG
                     DLLIRLISDDIGSITVGASVYFRKVPVGNIADYRFTKDQKKIEIDVVINQKYAHLVKK
                     ESRFWNISGISADVNLQGIKINMDSLASVVQGAVAFDSPDNMEIAEQGQKFELYPDLK
                     SAKRGIEINVKLPLTHNLKINETGVFHQNVQIGMLSQLKLPQVKDNGVPQQESGQHKQ
                     IDGVLLIDPSYENLLRSQTKILLKEPKFSLNTEQLTKVGELLRGVYFEVHPGEGEPQS
                     EFVVQNEADYLLSLPNLLSFSLIAPQAYGVDTNQGIYYSDVQIGEIVKRSLELDKVRF
                     DVIIYPPYRNLIGSNSKFVAISNIDMSVGLDGLRVHAGSPSEWLKGGVRLINGKNDGS
                     PKKQYALYKDIESAESGMATLDKKATTTLSADSLSGISEGSLVLYRDFQVGEVLKITP
                     KQQKFEVELFIQPNYRHLLTEKSRFWIEPATSVDVSLKGVNITTAPLMRTLKGAISFD
                     NGGSKGNKTLYASKEKATSGNTYLTLIAKDASKLSEGMDIKYMGLTVGQIEKLELQNA
                     KKQIKATAYIQRKYYPLIAKAGSKFNVISPEISTSGFKNLDAALQNYITVDVGSGQAQ
                     NQFVLADTDTVKTTYANGFPIIVETTNANGIQPEAPVLYRGMQVGIVSHLSLSEVNDR
                     VLIHLKINSKYQHLVRTNTQFWQASGYTMDVSLQGVSMNSGTMSQLLNGGIEFSTPYT
                     KVVKPQAQPNRRFFLQRKLPDEAPAWDQGIAE"
     gene            complement(15482..15922)
                     /gene="fur"
                     /locus_tag="PWY43_000483"
     CDS             complement(15482..15922)
                     /gene="fur"
                     /locus_tag="PWY43_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDL2135392.1"
                     /translation="MSEANIKRLKEVGLKVTEPRLTILALMQDIRDEMQHFAAEDIYK
                     ILLEKGSDIGLATVYRVLNQFEEAGILTRHNFDANKAVFELNMNHEHDHIICMDCGKV
                     FEFKDPDMERRQREISEKHGMELTNHSLYLYGKCSNLTNCDEKK"
     gene            complement(15958..16482)
                     /gene="fldA"
                     /locus_tag="PWY43_000484"
     CDS             complement(15958..16482)
                     /gene="fldA"
                     /locus_tag="PWY43_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDL2135393.1"
                     /translation="MAVVGLFYGSDTGNTENISKMIQKKLGAELIDIRDIAKSTKEDI
                     EAYDFLMFGIPTWYYGESQADWDDFMPTLKEIDFNGKVVAIFGCGDQEDYAEYFCDAM
                     GTVRDVVEPNGGVIVGHWPTAGYTFEVSQALVDDDTFVGLCIDEDRQPELTTERVNKW
                     CEQLKEEMCLDQLA"
     gene            complement(16488..16763)
                     /gene="ybfE"
                     /locus_tag="PWY43_000485"
     CDS             complement(16488..16763)
                     /gene="ybfE"
                     /locus_tag="PWY43_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDL2135394.1"
                     /translation="MAKQDSDCITLDLFATVPKVGRPRTNPLDREQQLRINKRNQLKR
                     DKSSGLKRVELKLHSDLVKLLEEQASEQGVSRGQLIETILNNYLTNR"
     gene            17004..17990
                     /locus_tag="PWY43_000486"
     CDS             17004..17990
                     /locus_tag="PWY43_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2135395.1"
                     /translation="MSNIHLAIAADFTLAEKLLEALANQDLGIENISAVELEPFGEEQ
                     NLRFGAKAVAQYALDEVNWAEFSHVLFAGKMAQAEVLAEAVKAGCVILDLYGITALIG
                     DVPVVVPSVNDENVANLRERNIVALANPQISQLALALKPLLNEPLKQVWVTSLLPSAY
                     FGDEKVKELAGQTARLLNGIPFDENAERIAFDVVPANVQGEEKSLPFSKAFELQIAKV
                     LPNLTACTTFHSVQVPVFYGVSQMVSVQSEYQLDANVLSEAWKAENWLNFNENSVITA
                     VKNGENEEQNALEISQLLAKSEQEVQFWTVADEQRFSLAFLAAKLLKLVLAY"
     gene            18087..19382
                     /locus_tag="PWY43_000487"
     CDS             18087..19382
                     /locus_tag="PWY43_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2135396.1"
                     /translation="MLEKLFQLKAKGSNVKTEIIAGITTFFTMVYIVFVNPSILGVAG
                     MDTQVVFVTTCLIAAFGTIAMGLFSNLPIALAPAMGLNAFFAFVVVQKLGYSWQVGIG
                     AIFLGSVGLFLLTVLQVRYWFMSAIPLGLRVGIGAGIGLFIALIGFKNMGLVVPNPAT
                     LVALGDLHDPKVLMGILGFFIIVVLAAKGIHSGVLISIVIVTALALAFDPAVSFNGIM
                     SMPPSLGAVVGQVDIAGAFDVALLGIIFSFMLVNLFDSSGTLIAVTTKAGFADEQGRF
                     PKMKQALLVDSTAAMAGSYMGTSAISTYIESGSGVSVGGRTGLTAVLVGILFLLTIFF
                     SPLAGLVPAYATAGALVFVGILMASSLIEVKWDDLTEATPAFITTAMMPFTYSITEGI
                     AFGFISYCVMKAFTGRWKEINLSVLVVSLLFIAKFVWVG"
     gene            19501..19869
                     /locus_tag="PWY43_000488"
     CDS             19501..19869
                     /locus_tag="PWY43_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HDL2135397.1"
                     /translation="MSNSVQKLLFIINSAPYGNETFFSALRLALQIQEQHKANTEIKL
                     FLMSDAVTAGLAKQNPAEGYHIQQMLEILTAQGANVKLCKTCTNARGITDLPLADGVE
                     IGTLIELADWTIQADKVLNF"
     gene            19869..21062
                     /locus_tag="PWY43_000489"
     CDS             19869..21062
                     /locus_tag="PWY43_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2135398.1"
                     /translation="MQNIDVVVIGAGAAGLFCAAQLGQAGKKVVVLDSGKKVGRKILM
                     SGGGFCNFTNLDVSAGNYLSQNRHFVKSALSRYTQWDFISLVSHYGISYHEKELGQLF
                     CDESSQQIVDLLQAECNKGKVEILLRQAVISVEKVANFFEISTACGKFQSEHLVITTG
                     GLSMPALGASPFGYQLAEQFGIPVVPPRASLVPFTWKESDKYFAELSGIALPITVSAN
                     GKSFSNQMLFTHRGLSGPAILQISNYWELGETVEIDLLPSQSITDILSELRQSSPKLQ
                     LKTVLSRYLPKKLVEVWLEQKLLTDKVIADLSKKDLTLLEQFIHHWHFLPNGTEGYRT
                     AEVTKGGVDTDYISSKTMEAKNTAGLYFIGEVLDVTGWLGGYNFQWAWSSAFACAEGI
                     IEKSK"
     gene            complement(21133..21351)
                     /gene="infA"
                     /locus_tag="PWY43_000490"
     CDS             complement(21133..21351)
                     /gene="infA"
                     /locus_tag="PWY43_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL2135399.1"
                     /translation="MAKEDCIEMQGTILETLPNTMFRVELENGHIVTAHISGKMRKNY
                     IRILTGDKVTVEMTPYDLSKARIIFRAR"
     gene            complement(21461..22120)
                     /locus_tag="PWY43_000491"
     CDS             complement(21461..22120)
                     /locus_tag="PWY43_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDL2135400.1"
                     /translation="MYISREKITKTLLLAGITLLCCGIVAVILMGVNQFKLGSQLASI
                     NQVSNLSHLLVRQQANIFSFLLVKNAKTDELVDALDSFAKEDFIIDANIYSPNGVLLA
                     QSQNAKDLKVLLDSEQSAKTQQIVEPIFSQQALVGFLRVTFDAEYGQTTKARVEQQFH
                     QLYGEIIILLLTGILLASSINYYFRRKIHHVQISPKPLMSQVKTQTQRFHSRRRWIKR
                     K"
     gene            22206..23069
                     /gene="serB"
                     /locus_tag="PWY43_000492"
     CDS             22206..23069
                     /gene="serB"
                     /locus_tag="PWY43_000492"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HDL2135401.1"
                     /translation="MTNTIFIYSKTLNEREIEQFSQQTAAILLKQAVYLDYKIAFFST
                     NLTACELRETAQKINADISDLTIIPTLNQGGLLLMDMDSTAIKIECIDEIAKLAGTGE
                     VVSAITASAMRGELDFEQSLRKRVGTLENASESILQKVRENLPLMDGFEQMVAILKEH
                     GWKLAIASGGFDYFADYLKQKYGLDFAVSNQLEIIDGKLTGKVLGKVVDAQHKADTLK
                     NLATTFNIPQNQWVAVGDGANDLPMLKTASLGVALHAKPKVQAQADFVVNFGDLTALC
                     LLLNANHLFQH"
     gene            23085..23576
                     /locus_tag="PWY43_000493"
     CDS             23085..23576
                     /locus_tag="PWY43_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL2135402.1"
                     /translation="MPSFDIVSEITMHEVRNAVENANRVLSTRYDFRGVEAVIELNEK
                     NESIKLTTESDFQLEQLIEILIGSCVKRGIEHSSLDIPTESEHHGKLYSKEIKLKQGI
                     ESDMAKKITKLIKDSKIKVQAQIQGEQVRVTGKSRDDLQAAIQLVKGADLGQPFQFNN
                     FRD"
     gene            23661..23837
                     /locus_tag="PWY43_000494"
     CDS             23661..23837
                     /locus_tag="PWY43_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5363 domain-containing protein"
                     /protein_id="HDL2135403.1"
                     /translation="MMSEQTEKKGWFKKALEKYDAFCDELGVNQGACRGCVPVVKFDP
                     EPEKEQPEKSENKT"
     gene            complement(23886..24182)
                     /locus_tag="PWY43_000495"
     CDS             complement(23886..24182)
                     /locus_tag="PWY43_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135404.1"
                     /translation="MKKHNIIILTLASASVVACRSNTPVQVVNGDKNIDYSQPKTIYI
                     SETNTQPPALTKQEKIKPSNATALCFNGSYSTDIQNPCKKDGGVKQYFAHHHAN"
     gene            complement(24259..25227)
                     /gene="mdh"
                     /locus_tag="PWY43_000496"
     CDS             complement(24259..25227)
                     /gene="mdh"
                     /locus_tag="PWY43_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDL2135405.1"
                     /translation="MCQSKKVALLGAAGGIGQSLALLLKLNLPAKSELSLYDISPVTP
                     GIAVDLSHIPTDVKVTGFAGEDPTEALKDADVVVISAGVARKPGMTRADLFNTNATIV
                     HNLVEKAAKVCPKACIAIITNPVNTIIPIAAEVLKKAGVYDKNKLFGVTTLDVIRANT
                     FVAEAKDVNVKYVRVPVIGGHSGTTILPLLSQATVNGLKLEFTQEQIEQLTHRIQNAG
                     TEVVEAKAGGGSATLSMAQAGAEFALGLVRALIGEDVIRYAYVDNANGETSPAFFAYP
                     IRLGTNGVEKVLPIGNLSEFEKDQLEQLIPVLNDEIQLGQTFNKNA"
     gene            25423..25884
                     /gene="argR"
                     /locus_tag="PWY43_000497"
     CDS             25423..25884
                     /gene="argR"
                     /locus_tag="PWY43_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDL2135406.1"
                     /translation="MDKIDSLSEAFKSLLKEEKFGSQSEIVAALQEMGFDNINQSKVS
                     RMLSKFGAVRTRNPKMEMVYQLPVELSVPTTSSPLKNLVVDIDHNDALIVVKTSPGAA
                     QLIARLLDSMGKAEGILGTIAGDDTIFITPVRNMQIEKLVKNVTQLFETSL"
     gene            25934..26830
                     /locus_tag="PWY43_000498"
     CDS             25934..26830
                     /locus_tag="PWY43_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDL2135407.1"
                     /translation="MHIFITGGTGFIGRELIQQLKSENHHFTLLTRDTEAKSGDLQIN
                     YCYSLDELVDLNDFDAVINLAGEPIFDKRWTDEQKQKLIESRVNLTNKLSQLMNASSN
                     PPHTFLSGSAIGIYGDLPPSANFYTESTACYQDCFTNRLCQQWEAAALKAASDKTRVC
                     LIRTGTVFSPEGGALKKILPLYRCGLGGKLGSGKQYWAWISRRDHVQAVIFLLKNANC
                     SGAYNFVAPNPVRNEEFNRILANAVNRPAFFTVPEFALKLGLGERACLVLDNQQLIPE
                     KLLAASFVFQDPILTENVFLNP"
     gene            26875..29073
                     /gene="uvrD"
                     /locus_tag="PWY43_000499"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             26875..29073
                     /gene="uvrD"
                     /locus_tag="PWY43_000499"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDL2135408.1"
                     /translation="MDFSLLLDGLNDKQREAVAAPIGNYLVLAGAGSGKTRVLTHRIA
                     WLIGVENVPESNILAVTFTNKAAAEMRHRIEYTLSQSGDNRLFGMWVGTFHSIANRLL
                     RSHYLDADLPQDFQIMDTEDQQRLLKRLLKLHNIDEKHFPPKHVAWYINAQKDKGKRA
                     KDIDHHNDPNEKKLVEIYQIYQDACDRAGLLDFAELLIRAYELFKTKPTILQRYQQRF
                     QQILIDEFQDTNNIQYDLIRLLAGDVGKVMIVGDDDQSIYGWRGAQVENIQRFLNDYD
                     NAQTIRLEQNYRSTGNILSCANELIANNDNRLGKNLWTDSGDGEPVDIYCAFNELDEA
                     RFVASQIKQWKEDEGDLAECAVLYRSNSQSRVIEEALIQANIPYRIYGGMRFFERQEI
                     KDALAYLRLIANRQDDAAFERVINTPTRGIGDRTLDTLRQITRGRQITLWQAIQVAVQ
                     EEQLSGRATSSLLRFVELINALDQETEQMSLAEQTDFVIKKSGLYEMYKQEKGEKGEV
                     RIENLEELVTATKQFVKPDEAEELTDLTAFLTHASLEAGEAQASQHEDYVELMTLHSA
                     KGLEFPRVFIVGVEEGIFPSGMSFDEGRLQEERRLAYVGITRAKKKLTISYAESRRLY
                     GKEERHVPSRFIAELPEEHIREVRLRGSINRAASFNQANSFAKNPTKTTASFLNDDTW
                     KMGQKVNHAKFGQGTIINVEGSGEATRLQVAFVGNGIKWLIAKMANLERI"
BASE COUNT         8591 a         5744 c         6346 g         8587 t
ORIGIN      
        1 ggttcaaaaa cggcttctcg tttggctttt aatcgtgggc taatcactca gggcgaagtt
       61 tggctagaaa tgataaaaag ccgcaatata acagttcata ctgacgatga aactatttta
      121 actgaagaat ttaataaaat tatcaaggcg tatctgcctt tatttattga atttgaacag
      181 cgagtagaaa cactatgcca aactttggac taagcgaacg agcgacgaaa actattaccg
      241 atattcttgc gaaataccct gaagtggagc aagccattat ttacggctcg cgtgctaaag
      301 gtaattatcg tgaaggctca gatgtagatc ttacttttaa aggaaaagat cttacttacg
      361 atattgtcaa acgcatttgg ggtgatttgg acgattccga ctcgccctat ttatttgact
      421 tgtcgattta tcatcaatta accaatgcta actttattga acatattgat agagtaggaa
      481 aagtctttta ccaaagagaa ctctaatttc ttaataggca agcggttcat tttgcaaaat
      541 tttttgcaaa tttcaccgct tgttgttttt attattaacc aatgtgcttc gccttaacca
      601 aagttacggg agatttatga caactcaatt aacctcaaaa acctacgaca ctcatttccc
      661 taaattaacc ccagaacaac ttgctgaaaa cagcaagaaa aaagtcatta tcggtatgtc
      721 cggtggggtg gattcctcgg tttccgcttt tattcttcaa cagcagggct accaagtgga
      781 aggcttgttt atgaaaaact gggaagaaga tgatgatacg gattattgta ccgccgccgc
      841 cgatcttgcc gatgctcaag cggttgccga taaactgggt atgaaactgc ataaaattaa
      901 ttttgcagcg gaatattggg ataatgtatt tgaatatttc ttggcagaat acaaggcagg
      961 aagaacgcca aatccggata ttttgtgtaa taaagagatc aaatttaaag cctttttgga
     1021 atatgcggca gaagatcttg gtgcggatta tattgcgacc gggcattacg tccgccgtac
     1081 gagcgatgat caagatgcga aacttttgcg tggcttagat gaaaacaaag atcagagtta
     1141 ctttttatat gcgttaaata aaaagcagat cgggcagagt ttattcccag tgggtgaaat
     1201 tgagaagccg attgtgcgag tcattgccga agacctagga ttagcaaccg caaagaaaaa
     1261 agattctacc ggcatttgtt ttatcggtga gcgtaaattt aaagatttct tagctcgttt
     1321 tctaccggct caaccggggg aaatccgcac cgttgagggc aaattagtcg gtcgccatga
     1381 tgggctgatg tatcacacgc ttgggcaacg caaaggcttg ggtatcggtg gggtgaaagg
     1441 gctaagtgaa gatccgtttt atgtggtgga gaaagactta attaacaatg tattgattgt
     1501 ggcacaagga cacgataatt cagccctgct ttcaagtggc ttaattgcga aacagctcac
     1561 ttgggtggat atgcagccgt tgcgtgaaaa tttacgctgc acggtaaaaa cccgttatcg
     1621 tcaagcagat attgcgtgtg aaatccaagt gcttgatgat gaaaccattc gagtgatgtt
     1681 tgatgaaccg caaattgccg tgacgccggg gcagtctgcc gtgttttact taggtgaagt
     1741 ttgcttaggc ggtggcatta ttgagtcaca attaaaatag ttataaataa atattgaaat
     1801 attttgcata tttatgcaaa ataagcgaaa agttaaggcg gtaaaacgcc tttttcattt
     1861 tgatcgatac aaagaataat atacaaggaa cagactatgg cactttgggg tggacgcttt
     1921 acacaacaag cagacgcaaa atttaaatat tttaacaatt cattacgttt tgattaccgc
     1981 ttggcgattc aagatattga aggctcaatc ggctgggcaa aagccattac ttcggtaggt
     2041 attttaacgg tagcagaatt agaacaacta atcgcagcat taaaagaggt gagagcagag
     2101 gtagaatcta accttaccat catcttaaaa gatgatgcgg aagacattca cagctgggtt
     2161 gaatctaagc tgattgaaaa agtgggcgat ttaggtaaaa aattgcacac cgggcgtagc
     2221 cgtaatgacc aagtagcggt ggatattaaa atgtggtgca aggtgcaagc ggtggcattg
     2281 caagaaagaa tccgagcttt gcaagagcgt ttggtttccg ttgccgaaga aaatcaagag
     2341 tcggtgatgc cgggctatac ccatttacaa cgagctcagc ctgtgacctt cgctcattgg
     2401 tgtatggcgt attatgaaat gctagaacgg gatttcagcc gtttgagtga tgctaataaa
     2461 cgtatgagca cttgcccgct tggttcaggg gcattggctg gtacggctta cggcattgat
     2521 cgtgacttat tagcccgtga tttaggcttt gaagaagcca cacgcaacag tttagacagc
     2581 gtgtcagatc gtgaccatat tttagagttg ctctctactg cctcgatcag tatgatgcac
     2641 ctctctcgct ttgctgaaga tttaatcatt tttaatagtg gcgaatcagg cttccttgaa
     2701 atgtcggatc gtgtgacctc aggctcatca cttatgccac agaagaaaaa ccctgatgct
     2761 tgtgagctga ttcgtgggaa atccggtcgg gtatttgggg cattaaacgg tttattaact
     2821 acactcaaag ggttaccact tgcttataat aaagatatgc aagaggacaa agagggtatt
     2881 tttgatgcgt tggaaacttg gcaggcttgt ttagaaattg cagaacttgt actggttgat
     2941 attcaagtga ataccgaacg cacccgagaa gccgctcagc aaggttatgc gaatgcgacc
     3001 gagttggcag attatttagt ggcgaaaggc gtgccattcc gtgaagctca tcatattgtt
     3061 ggcgaagcgg tggtttacgc aatcagcaaa aaagagccac ttgaagcatt aagcattaac
     3121 gaattccagc aattccatca ttcgattggc gatgatgttt acccaattct ctcgttagaa
     3181 tcttgcttag aaaaacgttg tgccaaaggt ggagtgaacc cacaacgtgt ggcagaggcg
     3241 attgcggtag ccaaagccaa tttagcttca aagtaaaacc ggatacaagc ggtcatattt
     3301 tgcaaaaaaa ttgaaaaata tgaccgcttg ccgattaaat tttcaatttt ttacgcatta
     3361 agattgcaaa ataggcatta cctctttaaa cttccgaata atttttgaat tttatttcta
     3421 ctactttctt taaagagttt tatatgacgg aatcagttaa ttctactcaa gctggtacag
     3481 cactttcttt agggcaacaa ttaaaagcgg ctcgtgaagc gttaaatctc tctattacag
     3541 atgttgctca aaaaaccaac ttaaaaaaat cacatattga atcacttgaa aatgatatct
     3601 ttattctaca aaatgttgcc ccaacttttg tgcgtggcta tgtgcgtaat tatgtgaaat
     3661 ttttgcgttt acctgaatcc cttatttcga gcgttaatta tggtgaagtc actattccaa
     3721 aagaagtcac taaagtttct tcagtaaaac cttcatcaaa ttcacaaggt aaaaccttaa
     3781 agtatttaac ggtattagta ttgcttgcgg cattaggaat gacattactt tggtggtggc
     3841 aagggtatca aaaagaacag gaaaatcgcg agcaatttgt tagctctgca ccagttgctg
     3901 aagtggcgaa taccgctaca aataatgatg cttcagcaca aacttccgtg cctgttcagc
     3961 aacctattca ggtttcagag cagcctgctc aagtagcaga aacggtagtt gcagcagaac
     4021 cggcattaac tgaaaaagta gagcctacac aagcagcatc atcacctgta acacaagaag
     4081 atgttaaagt ggtagaagaa gttaaacaag ctgatgccac cactgcacag gttgaggcag
     4141 ttaatgtttt acagcaatct caatcggaac aagcccctac agaacagcct gttgcggtaa
     4201 cgaatgatga gcttcgtatc gaaattaccg gttcacaaag ctggattaca gtgcggggtg
     4261 ctaaaaataa acgtttagca gaaaaattat ataacaatgg tgagacttta agtttcaatg
     4321 agaatgagca atatcgttta accattggtg cgcctgccaa tgtaaaagtg tactataaag
     4381 gtcaagaagt accattgaaa attgatgggc gtgtagctcg tattcgttta ccgttacagt
     4441 aagcttgatt taacgatgaa aaacgcacgt aaagctagca tttaacgata gctgtcttta
     4501 cgagcgtttt ctgtttttat aaattttcac aattagctaa aaatatgata catcaatcac
     4561 ctattaaacg tcgggtatcg aagaaaatct atgtgggtaa tgtgcctgta ggcggcgatg
     4621 caccgattac ggttcagtca atgaccaaca cccgcacaac cgatgtggaa gccaccgttg
     4681 ctcaaattaa atcgctggaa cgggtcgggg ctgatattgt gcgtgtgtct gtgccgacaa
     4741 tggacgctgc tgaggcattt aaagcgatta aacaacaagt gagtgtgcca ttggtggcgg
     4801 atatccactt cgattaccgc attgcgttga aagttgccga atatggggtg gattgcttgc
     4861 ggattaaccc gggtaatatc gggaatgaag agcggatccg cacggtggta gattgtgcca
     4921 gagataaaaa cattccgatc cgaatcgggg taaatgccgg atcattagag cgtgatttgc
     4981 aggaaaaata tggcgagccg acaccacagg cattattaga atcggcatta cgccacgttg
     5041 atcacttaga tcgccttaac tttgatcaat tcaaagtcag cgtgaaagcc tcagatgtat
     5101 ttctagcggt tgaatcttac cgacttcttg caaaacagat tgatcagccc ctgcatttag
     5161 gtattactga agcaggcgga gcgagagccg gttcggtgaa atcggcggta ggcttaggtc
     5221 tgttgctagc ggaagggatt ggtgatacct tgcgtgtgtc attagcggct gatcctgttg
     5281 aagaggtgaa agtcggtttt gatattttga aatcgctgcg tattcgctct cgtgggatca
     5341 actttattgc ttgcccaacc tgctcccgcc aagaaattga tgtgattgcg acggtaaacg
     5401 agctagaaca acgtttagaa gacattatca cgccgatgga cgtttcgatt atcggctgtg
     5461 tggtaaacgg tccaggagag gcgttggtgt ctgatttagg ggtaaccggc agcaataaaa
     5521 tgagcggctt ttatttagat ggcgttcgtc aaaaagagcg ttttgacaac gcaaaattaa
     5581 tcgaccaatt agaagctaaa attcgtgcca gagttgcaca acaacataat cgtatagata
     5641 ttgaacaaat aggataaata tagtggcaaa aacaattcaa gcaattcgag gaatgaacga
     5701 ctgctcacca agtgagtctc cattatggca atgggttgaa cagaaagtgc gtaatgtgct
     5761 ggcaagttac ggctattcgg aagtacgtat gccgattgta gaaagcactc cgttatttgc
     5821 ccgtgccatt ggcgaagtaa ccgatgtggt gtcgaaagag atgtacacgt tctgggataa
     5881 cgatgagcaa ttaacgctcc gcccggaagg tacagccggt tgtgtgcgtg cagcgattga
     5941 gcgtggttgg atctacaata atgagcaacg cttatggtat atggggccga tgttccgcca
     6001 cgaacgtcca cagaaagggc gttaccgcca attccaccaa gccggtgttg aagtgtttgg
     6061 cattgcaaat ccggaaattg atgcggagtt gattattctc accgcacgcc tatggaaaga
     6121 gttaggcatc gatcaacatg tttctttgca acttaactct atcggctcgt tagaagctcg
     6181 tgcaaattat cgctcagcat tagtaaaatt tttagaaaat cacaccgctt taatgaacga
     6241 agaagagaaa gagcgtttgg tgaaaaaccc gctacggatt ttagatacca aaaacgaagc
     6301 actacaaaat gtattaaacg atgccccaaa actgctcgat tatttagatg atgacagccg
     6361 tgaacatttt acccaacttt gtgccttact tgatgcaatg ggaatccgtt acgaagtcaa
     6421 tccaaaattg gtgcgtggtt tagactatta caataaaacc gtgtttgaat gggtcacttc
     6481 agccttagcc ttaggctcac aaggcaccgt atgtggcggt gggcgttatg acggcttagt
     6541 cgaacaactc ggtggacacg caacccaagg cgttggcttt gcaatgggct tagagcgttt
     6601 agtgctgttg gtacaagagg tgaataaaga gattgattta ccaaaaacgg tggatgtgta
     6661 tatcgtattc tccggtgaag gggcaacact aaatgccttc caacttgcag agcagattcg
     6721 tagcgaattg ccacacgttg gcgtaatgac acactgttcc ggcggtaaat tccaaaaaca
     6781 atttaagcgt gcagataaag tagaagcgca aattgcgtta gtgattggcg aaagcgaagt
     6841 ggctgaaaag caagttgtta tcaaagattt acgttcagga gcagaacaaa tcacgattgc
     6901 tcaatcagag ctgattgctg agctatctaa acgattttag gagaaaaaga tgagtaacga
     6961 atatttaaat aaaaccgaag agcagcaatt tgaagatgcc aaaaattggt ttaaagagaa
     7021 cgggacaccg attttggtgg cgattatttt agcttgtgcg gcaactgccg gctggaattt
     7081 ttggaaaaat caccagcttg aaacggcaca aaaaacatcc gcttcatacc aaagcgtgat
     7141 ggagagttat ttgcaaaatc caagcaaaaa tcaaccgctt gttgagaaat ttattgccga
     7201 taataaagat tcaagctatg ccgcttttgc ccaatttgaa gtagctaaac aagcggtcga
     7261 aaaagggaat ttttctgcgg caaaaacagc gttacagtca gttttgactt attcggattc
     7321 aaccttacaa tctatcgctc gttttcgttt ggctcaaatt gacttccaac taaaatctta
     7381 cgatgaagca ctggcaagtc tggctcaaat tcaagataaa gcgtgggaat tgcgtaagca
     7441 aattctaaca ggcgatattc ttgcagcaaa aggcgatatt cctgcggcga aaagtgctta
     7501 tgagcaagct aaaacgaccg caaatcctca ggaacaaacc ttaattgatg tacgtttgaa
     7561 taacttataa aaacaaagcc tgattgaaat aaaatcaatc aggctttttt gttggtttag
     7621 tgttgctgtg aattataaac gcattggcat aatcacatat tcggcagagc tgttgtcgca
     7681 atcttcaatt aagcaactag aacttgaatc aactaagcta aaacgcacac gttggcattt
     7741 taaggtgttt aacacatcta atacatagct aacgttaaag ccgacttcca tttccgggct
     7801 gtcgtaggca acatcaatga tctcttccgc ctcttcttgc tccggattgt ttgcggtaat
     7861 tttgagtaaa ttattgctta aggttaaacg cacccctcta aatttctcat tagaaagaat
     7921 cgctgcacgc attaatgccc gtttaagggt ttcggtttcc gcttctagaa tgcgatctgc
     7981 attgcgaggc agaactcggc ggtaatccgg gaatctgcca tcaatcagtt tagaggtaaa
     8041 tacaatgccg ttcattgtaa tacggatatt gcttgaaccg atttccaaac gcacggtttc
     8101 atcgttgctg gcaaccaagc gggaaagctc aagtacggct ttgcgtggca cgataaccga
     8161 atgagtcaaa agctcttgat ttagcaccat tgtgcaaacc gctaaacggt gcccatctgt
     8221 ggcaacggtg cgtaataaat tgccttcggt ttcaaatttc attccgttta agaaataacg
     8281 ggcgtcttgg tttgccattg agaattgggt ggcatcaatc agacgggcaa gggtagattg
     8341 atcaatcgag aaatccactt ccggtttcca atccatcaaa ttcgggtaat cacttgccgg
     8401 taatgtagct aaactaaatt tactacgtcc tgctttgata atggctttat cttcttcaaa
     8461 ttgtaccgaa atcacgctgt catcaggtag gcttttactg atatctagga attttttagc
     8521 tggaatggta aattttccgg caaaatcgac cgcttgtagc agttgtgctt gggtggttaa
     8581 ttccacttct aaatcggtac cggttaaaga tagacgatta ccttcaattt ctaataaaag
     8641 attgttaata acaggcaaag tcggacggct acttaatacg ccacacacct gttgtaaagg
     8701 cttaagtaat tgttctctgg taatggtaat ttgcataatc attccttcat cgggctaaga
     8761 tgataatttg cgtgttaaat ggatataatc ttcttggata ccgctttcgg tatcgcgtaa
     8821 ttcattaatg gttttgcagg cgtgcatcac ggtagtatga tcccgaccgc caaattcccg
     8881 accaatttcc ggcaagctgt ggttggttag ctcttttgcc agtgccattg ccatttggcg
     8941 aggacgggca atgcttcttg cccgactttt cgatttaata tcggcaactt ttatgccgta
     9001 atattcggcc acaatgcgtt gaatattttc aatcgtaatc aagtaatcaa aagaggcaaa
     9061 cagatcttgc aaggtttcac gcaccgcatc aaccgtaatc gggcgaaggg tcaatttttg
     9121 ccacgcaatc acacggttta atgctccctc caattcacgc acattggttc ggagtttttg
     9181 ccccattaaa taggcggcat cttcttgcaa atctgctcca cgttctaagg ctttttggcg
     9241 aagaattgcc actcgggttt ccaattcagg tggctcaatg gtcgcactaa taccgccgct
     9301 taaacgggat ttaatcgccg tgtcaatttt atcaatattt ttcggcaaca catcggaggt
     9361 taaaataatc tgtttgcgtt gttcaaacag cgaatttaac agatgtaaaa attcttcttg
     9421 tactttcggt ttattggcaa gaaattgaat atcgtcaatc attaacacat caagcgagcg
     9481 gtagaatttt ttgatttttt ccgcatcgtt attggaactg ttgatcgcct taataatatc
     9541 acgataaaaa cgttccaagt gaatatatag cacccgagta cccggtgtgc gtttaactaa
     9601 ttcattgccg atagcgtgca ataagtgggt tttgcctaaa ccggtgccgg cataaaacga
     9661 gagtgggttg caatggcttt cgcctaaatt atccaccact tgttgagcca ctaatttagc
     9721 taattggttt gagcggcctt gtacaaaatt atcaaaggtt tgatttgggt ttagcccggt
     9781 tttaaagccg gtattgctaa attctgaact ctcgtttttt agatttgggc tgacctttgg
     9841 ctgttctgtc ggtttaatcc caacttttaa cgtcaccacc aaattatcat ttttcgccaa
     9901 aaaacgggca agctcggtaa tctccgataa aaatttatct tttacccagt tttcaacaaa
     9961 ttgattttgt gcataaagtg tgagttcccc attctcagaa ctcgcttgca gaggacgcag
    10021 ccaagtgctg taatcggttg gtgagacttt cgtttgtaag tgattaaggc aatcagacca
    10081 aagggaagac acaatttgct ccaaaattat tgttattaaa ggtcataatg atacctgaaa
    10141 aaaggggaga aatcttgcaa atttttgata gattttattg gtgtttggaa gcggttttaa
    10201 ttggcgaaca agcggtcgtt ttttgttgaa atcttgcaaa ttgctaacaa aaaatgaccg
    10261 cttgcttagt atgatgtgtg atggagttga ttaaatatcc gtattctccg gtaagaaccc
    10321 cccgctttgg tgagcccaaa gtctggcgta aacaccgttt tgagcaagca attcttcgtg
    10381 cgtgccttgt tcaacgattt cgcctttatc taacacgata agtctgtcca ttgccataat
    10441 ggtagagagg cggtgggcaa tcgcaaccac ggttttacct tccattaatt gcgtgagatt
    10501 ttcttgaatt gccacttcta cttcagagtc taacgcactg gttgcctcat cgagtaataa
    10561 aatcggagca tcttttagca tcactcgggc aatcgcaatc cgttggcgtt gtccgccgga
    10621 aagttttacc cctcgttcgc caacgtgtgc atcaaaaccg gttctgcctt tggcatcttg
    10681 caggtttgga ataaattcgc tagcggcagc ttgttcaacg gctttgatca tctcttcttc
    10741 ggtggcgttc gggcgaccgt acatcaagtt ttcacgcaca gagcgatgta acagcgaggt
    10801 gtcttgagtc accaaaccaa tttgtgagcg aaggctttct tgggtaatat cacggatatt
    10861 ttgcccgtca atcgtgattg aaccgtcttg aatatcgtaa aaacgcaata ataaattggt
    10921 gagcgttgat ttacctgcac cactacgccc gactaaaccg actttttccc ccggttttat
    10981 agtgaggtca aaatctttca gtagcggttt ttttggatcg taggcaaaat ccacattctc
    11041 aaatttaatt tcgcctttgg ttacatttaa cggtttcgca tcgattttat ctaccacatt
    11101 gtgtggtttg gaaagcgtaa tcataccgtc ttgtaccgta cctaaatttt caaatagacg
    11161 agcaaattcc cacataatcc attgtgataa gcctttaatg cgtaaagcca aagcggttga
    11221 ggtggcaatc gcccctgcag ttactacgct tgaaccccaa agccataaac cgatacccgc
    11281 agttgaaatt attaatgcca tcgaggttag attagttacg gtttcaatta cagtcactaa
    11341 acgcatctgt ttatgcactg tcaccataaa ttcttccatc gactctttcg cataggcaga
    11401 ttcacggttg ccgtgcgaga agagtttgac ggtggcaatg ttagcgtaag catcggtaat
    11461 tcgaccggtc attaaacttc tggcatccga ttgctcttga gcagcttggg ctaatttcgg
    11521 gacaaagtag ataatggttg ccgctaatga gatgacccat aaaataaacg gcacgaataa
    11581 ccagccgtca agttgtaata aaatcacgct ggaggtggtg aagtaaacca gcacatacac
    11641 caacatatcg gcacaagtca taatcacatc acgcacggaa agggcggttt gcattacttt
    11701 tgcagacact cttcctgcaa attcgtcttg atagaaacct aaactttgcc caagcattaa
    11761 gcggtggaag ttccaacgca aacgcattgg gaatacccct tgcagagatt gaaagcgaat
    11821 gctgctggca aggtaaacaa acaccactgc aagtagggca ataaaaaaca taccgataat
    11881 cgtccagcct ttttctgccc aaagttctgt tggcgagtat ttattgaccc aatcgactaa
    11941 atcgcccata aagcgaaaca gaaccgcttc gataataccg acagctgcaa ctaatacggt
    12001 cagtagtaag atataaaacc gcattccttt agtggcatcg taaataaaag gaatgagacc
    12061 tgattttggg gttcttggcg tttcatttgg gtaagtttcc acccttgcct caaaccattg
    12121 aaacagtttt tcaaacataa atttgtgatt tccgaataaa atgcgggggc aaataaccat
    12181 ttgcccccga aaagatgtta aaaaattatg gattaagtaa tttttcgtct aaggctaaat
    12241 cttcgttgcg atttacaccg atgccggcag ctaacacatt acgagcaatt tggtgagctt
    12301 cttctaaaga atgctctttg taagagccac attgatattc gttcagctca ggaattgccg
    12361 atacatcagg gattttatct aacgcatctt ccattgaagc cgtccacgct ttgacaacct
    12421 cttctgctga aggcgtgccg atgagcgaca tataaaaacc ggtacggcag cccattggcg
    12481 aaatatcaat gatctccact tgttcgctat tgaggtggtc acgcataaag ccggcaaaaa
    12541 gatgttccat tgtgtgaatc ccacgcaccg gtagaatatc aatattcggg cggcagaaac
    12601 ggagatcgaa cacggtaatg gtatcgcctt tcggtgtggt cattgttttt gcaacacgca
    12661 cggctggggc gttcattttg gtgtgatcta ctttaaaact atctaataaa ggcatttttt
    12721 actccatcaa tattattccg caattccttg atcccaagca ggggcttcat caggtagttt
    12781 gcgttgtaag aaaaatcgtc tgtttggctg agcttgaggc ttcaccactt tagtgtaagg
    12841 ggtagagaac tcaataccgc cgtttaataa ctgcgacatt gtgcctgagt tcatacttac
    12901 accttgcagg ctcacgtcca tcgtgtaacc tgaagcttgc cagaattggg tattggttct
    12961 gactaagtgc tgatatttgc tattaatttt caggtgaatt aaaactctat catttacttc
    13021 gctgaggctt aaatggctga caatccccac ctgcattcca cgataaagca caggtgcttc
    13081 tggctgaata ccattggcat tagtggtttc aacaataatc gggaaaccgt tggcataggt
    13141 ggtttttacc gtatcggtat ctgcaagaac aaattggttt tgagcttgcc ctgaaccgac
    13201 atcaaccgta atgtaatttt gcagtgctgc atctaaattt ttaaagccgg aggtactgat
    13261 ttcaggcgag ataacattaa atttactgcc ggcttttgca attaacggat agtatttcct
    13321 ttgaatgtaa gcggtcgctt ttatctgttt ttttgcattt tgtagctcaa gtttttcaat
    13381 ttgacctacg gttaagccca tatatttaat gtccattcct tcgcttaact tactcgcatc
    13441 ttttgcaatc agcgtgagat aagtattgcc cgaagtggct ttctccttac ttgcataaag
    13501 cgttttattg ccttttgaac cgccattatc aaagctgatc gcccctttta aggtacgcat
    13561 taatggagct gtagtgatat tcacgccttt gagtgacaca tcaacactcg ttgccggctc
    13621 aatccagaaa cggcttttct cggttaatag atgacgataa tttggctgga taaatagctc
    13681 aacctcaaat ttttgttgtt tcggggttat ttttaacacc tcgccgacct gaaaatcacg
    13741 atatagcacc aaagagcctt cgctgatacc agataatgaa tctgccgaaa gggtagtggt
    13801 ggctttttta tctaaggtag ccatgcctga ttccgcactt tctatatctt tataaagtgc
    13861 atattgtttt ttcgggctac cgtcattttt accgttaatt aagcgaacac cgccttttaa
    13921 ccattcagat ggtgaaccgg cgtgaactct taacccatct agccccaccg acatatcaat
    13981 gttggatatc gcaacaaatt tgctgttact cccaatcagg ttgcgataag gcggataaat
    14041 aatgacatca aaacgtactt tgtctaactc taggctacgt ttgacgattt ctccgatttg
    14101 cacatcgcta tagtaaatcc cctgattggt atctacccca taggcttgag gagcaattaa
    14161 gctaaagcta agtaaattcg gtaaacttaa tagataatcg gcttcgtttt gtaccacaaa
    14221 ttccgattga ggttctcctt cccccgggtg aacctcaaaa tagacaccac gcagcagctc
    14281 acctactttc gtcagctgct ctgtgtttaa ggagaatttc ggctctttga gcaagatttt
    14341 agtttgggag cgtagtaaat tttcataact tgggtcaatt aataaaaccc catcaatctg
    14401 tttatgctgc cctgattctt gctgtggcac accgttgtct tttacttgcg gtaattttag
    14461 ctgcgaaagc ataccaattt gcacattttg gtgaaaaaca ccagtctcat taatttttaa
    14521 attatgtgta agcggtagtt ttacgttgat ttcaattcca cgtttagctg atttcaaatc
    14581 cggatatagc tcaaatttct gtccttgctc ggcaatttcc atattatcag gcgaatcaaa
    14641 agcaaccgca ccttgtacta ccgaggcaag gctgtccata ttgattttaa taccttgtaa
    14701 atttacatca gcgctaatac cgctaatatt ccaaaatcgg ctctcttttt tcactaaatg
    14761 agcgtatttt tgattaatga cgacatcaat ttcgattttt ttctggtctt tggtaaaacg
    14821 ataatcggca atattaccca ctggcacttt acggaaataa acactcgctc cgacagtaat
    14881 agagcctatg tcatcagaga ttaaacggat cagtaaatcg ccatcgctca cggtaatagc
    14941 cggaggatct tcttctgcaa taaattcatc aacgaaatcg ccttcaccgg gaagtagggt
    15001 aatatagtta ccagaaacaa tcgcatctaa gcctgaaacg ccagccaaag aagcacttgg
    15061 ttgcactaac caaaatttag tctgtttgcg taaaatactt ttggcttcag ggtttatttc
    15121 tgcttgcact tccacttctt ttaaattatc gacaaaataa acacgcttaa cctgaccaat
    15181 ctgcaagcct tggtaacgaa tagcggtttt tccggcagta atgccatcac ctttactaaa
    15241 tcgaatcgtg attgtttcac cttgctcttt taaaatttgg aaaaatagca aagcaccaat
    15301 aataaaagca actattggta acagccaaaa aggagaaatt ttgcgaggct gccggatttg
    15361 ggcatctaca acagtgtggg tattttcttc catcgtcatt ttaaattagg gaaacctata
    15421 tagaacaata ccgaagaccc aaagggactt cggtaataga aataagtcaa aataagaaat
    15481 attatttttt ctcatcacag ttggttaaat tactgcattt accatacaga tataaactgt
    15541 ggttagtcaa ttccattccg tgtttttcac tgatttcacg ttggcgacgc tccatatcag
    15601 gatctttaaa ttcgaacacc ttaccacaat ccatacaaat aatatggtcg tgttcgtggt
    15661 tcatatttaa ttcgaatact gctttattcg catcgaaatt atggcgggtt aaaatgccgg
    15721 cttcttcaaa ttggtttaag acacgataaa ccgttgctaa accaatgtct gagccttttt
    15781 ctaacagaat cttataaata tcttctgctg caaaatgttg catttcatca cgaatatctt
    15841 gcattaatgc aaggatagtc aaacgaggct cggttacttt taagcctacc tcttttaaac
    15901 gtttaatatt tgcttcagac atatatgcca cctatttatc tttatccaaa atagttatta
    15961 tgcgagttgg tctaagcaca tttcttcttt caactgctca caccatttat taacacgttc
    16021 agtggttaat tcaggctgac gatcttcatc aatacataag ccgacaaaag tatcatcatc
    16081 aactaaggcc tgagaaacct cgaaagtgta accggcagtt ggccaatgac caacaatcac
    16141 accaccattt ggctctacca catcacgcac agtacccatt gcatcacaga aatattctgc
    16201 ataatcttcc tgatcaccgc aaccaaaaat tgcaacgact ttgccgttaa aatcaatctc
    16261 ttttagggta ggcataaaat cgtcccaatc ggcttgcgat tcaccataat accaagttgg
    16321 gatcccaaac atcagaaaat cgtatgcttc aatatcttct tttgtgcttt tggcaatgtc
    16381 tcgaatatca attaattcag cccctaattt tttctgaatc atttttgaaa tattttctgt
    16441 attgcctgta tcgctaccgt aaaataaacc tacaactgcc attgctacta cctatttgtg
    16501 agataattat ttagaattgt ttcaattaac tgcccgcgtg aaacaccttg ttcagacgcc
    16561 tgttcctcta ataactttac taaatccgaa tgcagtttta attctacccg ttttaaaccg
    16621 gaagatttat ctcgttttag ttggttacgt ttattgattc gaagttgttg ttctctatca
    16681 agaggatttg tacgaggacg cccaactttt ggtacagtcg caaacagatc taaggttata
    16741 caatctgaat cttgttttgc cattttgttt tactattggg ctttagctaa tcctcagttt
    16801 taatgagaat gataagctac ttttaaagtg tgaactatat caaaagttca atcatttgaa
    16861 aagtaaatta caggcaaaac aagcggtgtt tttaggttta aaatttgcaa aaatcataaa
    16921 ttacacatta tgagttatga tttttctgaa gcgtgaaatt agcttataat gaaaccgaat
    16981 aacgttatga aaaaggagat agtgtgtcga atatccattt agcgattgcc gctgatttta
    17041 cgctggcaga aaaattactt gaagcattag caaatcaaga tttagggatt gaaaatattt
    17101 cagccgttga gcttgagccg tttggtgaag agcagaattt acgctttggg gcaaaagcag
    17161 tcgcacaata tgcgttagat gaagtaaatt gggcggaatt tagccatgtt ctatttgccg
    17221 gcaaaatggc acaagcggaa gtgttggcag aagcggtgaa agccggctgt gtgattttag
    17281 atttatacgg cattaccgca ctcatcggcg atgtaccggt agttgtgcca agtgtaaatg
    17341 atgaaaatgt cgcaaatttg cgtgagcgaa atatcgttgc tttagccaat ccgcaaattt
    17401 cacaactggc gttagcctta aagccattgt tgaacgagcc gttaaaacag gtgtgggtaa
    17461 cctcactttt accgtctgcc tattttggcg atgaaaaagt caaggagctt gccggacaaa
    17521 ctgcccgttt gttaaacggc attccgtttg atgaaaatgc ggaacgcatt gcctttgatg
    17581 ttgtgccggc taatgtgcaa ggagaagaga aaagtctgcc gttctcaaaa gcctttgaac
    17641 tgcaaattgc aaaagtttta ccaaatttga ccgcttgtac taccttccac agcgtgcaag
    17701 tgccggtctt ttatggggtt tcccaaatgg tgtcggtgca gtctgaatat cagttagatg
    17761 ctaatgtgtt aagtgaagct tggaaagccg aaaattggct gaattttaat gaaaattcag
    17821 tgattacagc ggtgaaaaat ggcgaaaacg aagagcaaaa tgcgttagaa atcagtcagt
    17881 tattggcgaa atccgaacag gaagtgcagt tctggacggt cgccgatgaa cagcgtttca
    17941 gtctggcatt tttagcggcg aaattactaa aattagtttt agcgtattaa gtgcttgcaa
    18001 aagtatttat tccgtgataa aaatcgtcaa cgcccgagca accgattgct tgggcgttgt
    18061 cattttgttt aaacaccgaa ggttttatgt tagaaaaatt atttcagctc aaagcaaaag
    18121 gcagtaatgt aaaaaccgaa attatcgccg gtattactac ctttttcaca atggtttata
    18181 ttgttttcgt caatccgtct attttaggcg tggcgggaat ggacacccaa gtagtgttcg
    18241 tgactacttg tttaattgcc gcttttggta ctatcgcaat ggggttattc agtaatttac
    18301 cgattgctct tgcaccggca atgggcttaa atgccttttt tgcattcgta gtggtgcaaa
    18361 aattaggtta ctcgtggcaa gtgggtatag gggcgatttt cttaggctct gttggcttat
    18421 tcctattaac tgtattacaa gttcgttact ggtttatgtc tgcgattcca cttggtttac
    18481 gtgtaggtat tggtgccggt atcggtttat tcattgccct aatcggtttt aaaaatatgg
    18541 gcttggtggt gccaaatccg gcaacgcttg tggcactagg tgatttgcac gacccgaaag
    18601 tgttaatggg gattttaggc tttttcatca ttgttgtatt agcggcgaaa ggtattcact
    18661 caggcgtgtt aatttcaatt gtgattgtta ccgctcttgc cttagccttt gacccggcgg
    18721 tgagctttaa cggcataatg tctatgccac caagccttgg ggcagttgtg gggcaggttg
    18781 atattgccgg tgcttttgat gttgccttac tcgggatcat tttctccttt atgttggtaa
    18841 acctgtttga ctcttccggc accttaattg cggtgactac caaagcaggc tttgcggatg
    18901 aacaagggcg tttcccgaaa atgaaacaag ctctgctagt agatagtacc gctgcaatgg
    18961 caggctctta tatgggaact tcagcgattt ctacctatat tgaaagtggc tccggcgttt
    19021 cagttggcgg tcgtaccggc ttaactgcgg tgttagttgg gatcttattc ctattaacca
    19081 ttttcttctc accacttgca gggttagtac ctgcctatgc aactgcgggg gcattggtat
    19141 ttgtaggaat tttaatggca tctagcttaa ttgaagtaaa atgggacgat ttaactgaag
    19201 ctacgccggc atttattact accgcaatga tgccgtttac ttactccatc actgaaggga
    19261 ttgcattcgg ctttatcagc tactgcgtaa tgaaagcctt tacagggcgt tggaaagaaa
    19321 tcaacctttc tgttttagtg gtttcacttc tctttattgc taaatttgtg tgggtgggtt
    19381 aagctctacc ccctctccct gatttttctt ctaaacgaag aaaaatctat ccctctccca
    19441 caaaggggag agggtatttg attcattatc tatgtgtttc gtgtattccg aggtaaaaca
    19501 atgtctaatt cagttcaaaa acttcttttt atcatcaatt ctgctcctta cggcaatgaa
    19561 acgtttttca gtgcgttgcg tttggcatta caaattcaag aacaacataa agccaatacg
    19621 gaaattaaac ttttcttaat gtcggatgca gtcaccgcag ggctggcaaa gcaaaatccg
    19681 gcagagggtt atcatattca gcaaatgctg gaaattttaa ccgcccaagg ggcaaacgta
    19741 aaattatgca aaacctgtac taatgctcga gggattacag atttgccttt agccgatggg
    19801 gtagagattg gcacattaat tgaactggca gattggacaa ttcaagcaga taaggtactg
    19861 aatttttaat gcaaaatatt gatgttgtgg tgattggtgc cggtgcggca gggctgtttt
    19921 gtgctgccca actggggcaa gctggcaaaa aagtggtggt gttagacagc ggtaagaaag
    19981 tcggacgtaa aattttaatg tctggcggtg gtttttgcaa ttttacgaat ttagacgtaa
    20041 gtgccggaaa ttaccttagc caaaaccgcc attttgtgaa atccgccctt tcacgttata
    20101 cccaatggga ttttatttcg cttgtcagcc actatggcat tagctatcac gaaaaagagc
    20161 tagggcagtt gttttgtgat gaaagctcgc aacaaattgt cgatttacta caagcagaat
    20221 gcaacaaagg caaggttgag attctgttac ggcaagcggt catttctgtg gaaaaagttg
    20281 caaatttttt tgaaatttcg accgcttgtg gcaagttcca atccgaacac ttagtgatta
    20341 caaccggagg gctttcaatg ccggctctcg gggcaagccc ttttggctat caacttgccg
    20401 agcagtttgg tattcctgtt gtgccaccac gtgcaagcct tgtgcctttt acttggaaag
    20461 agagcgataa atattttgcc gagctttccg gcattgcact gccgattact gtttccgcca
    20521 atggcaaaag ttttagcaat cagatgttgt ttacgcacag aggcttatcc ggcccggcaa
    20581 ttttgcagat ttccaattat tgggaattgg gggaaacggt agagattgat ctgctacctt
    20641 cgcagtccat taccgatata ttgagcgaac tacgccaatc ttcgccgaaa ttacagctaa
    20701 aaaccgtatt atctcgttat ttacctaaaa aattggttga agtttggctg gaacagaaat
    20761 tgctgaccga taaagtgatt gccgatttga gtaaaaaaga tctgacctta ttagagcaat
    20821 ttatccacca ttggcatttc ttgccaaacg gcacggaagg ctatcgtaca gcggaagtaa
    20881 ccaaaggcgg tgttgatacc gactatattt catctaaaac gatggaagcg aaaaatacag
    20941 cagggcttta ttttatcggc gaagtgctgg atgttaccgg ctggcttggt ggttataatt
    21001 tccaatgggc gtggtcatct gcttttgcct gtgcagaagg gattattgaa aaaagcaaat
    21061 aactaaaaaa gcaaataact aaaaaaccaa acaaaaaggc gaacacaatt gttcgcctta
    21121 ttttatgctg aattatcttg cacggaaaat aatgcgtgct ttgcttaaat cgtatggagt
    21181 catttcaacc gttactttat caccggttaa aatacgaatg tagtttttac gcattttgcc
    21241 tgaaatatgt gcggtaacaa tatgaccgtt ttctaattcc acacggaaca ttgtatttgg
    21301 taaggtttct aaaatcgtac cttgcatctc aatgcaatct tctttagcca tttattcctc
    21361 tttaaagttt taatttttat atgatgccat tcggcgaaaa acggcggata ttatacgctt
    21421 attcgctcaa attacaatcg tttatccatt tgagtgtaaa ctatttacgc ttaatccaac
    21481 ggcgacgtga gtgaaaacgc tgggtttggg tttttacttg actcataagt ggttttgggc
    21541 ttatctgtac atgatgaatt ttacgacgaa aatagtagtt aatgctactt gccaacaata
    21601 tacctgtgag tagcaaaata attatttccc cataaagttg atgaaattgt tgctctactc
    21661 gtgctttagt agtttgccca tattctgcat caaacgtgac gcgtaaaaag ccaaccaacg
    21721 cctgttgaga aaatattggc tcaacaattt gctgagtttt ggcagattgc tctgaatcga
    21781 gcaatacttt taaatctttc gcattttggc tttgagccag tagcacgcca tttggtgaat
    21841 agatattggc atcaataata aaatcttctt tggcaaaact atccaacgca tcaaccagct
    21901 catcggtttt ggcattttta actaacaaaa acgaaaaaat attcgcttgc tggcgaacca
    21961 ataggtgtga aaggtttgat acttgattaa tgctggctaa ttgtgagcct agcttaaatt
    22021 ggtttacgcc cattaaaata acagccacga tcccacagca gagtagcgta atccctgcca
    22081 gcaaaagcgt tttcgttatt ttttcacgac taatgtacat tgaaattccc tcaaaatgat
    22141 gcgtgattgt agcacgcttt tctgttagat tagtgcagta aatttaaaca acctaaaata
    22201 agacaatgac aaacacaatt tttatctatt caaaaacctt aaacgagcgt gaaattgaac
    22261 agttttcaca acaaacggca gcaattttgc tgaaacaagc ggtctattta gactacaaaa
    22321 ttgcattttt ttccacaaat ttaaccgctt gcgagctgcg tgaaacggca cagaaaatca
    22381 atgcggatat tagcgatctc actattattc caaccttaaa ccaaggcggt ttgctattaa
    22441 tggatatgga ttccaccgct attaaaattg aatgtattga tgagattgcc aaacttgccg
    22501 gcaccggcga ggtggtgtcc gccattactg ccagtgcaat gcggggcgag ctggatttcg
    22561 agcaaagttt acgcaagcga gtcggcacgc tggaaaatgc atcggaaagc attttgcaaa
    22621 aagtgcggga aaatctaccg cttatggacg gctttgagca gatggttgct attcttaagg
    22681 aacacggctg gaaattagcg attgcgtccg gtggatttga ttacttcgcc gattacttaa
    22741 aacaaaaata cgggttggat tttgcggtct ctaaccaact ggaaattatt gacggtaaat
    22801 taaccggcaa ggtgttaggc aaagtagtgg atgctcaaca taaagccgat accttaaaaa
    22861 atttggcaac aacctttaat attccacaaa atcaatgggt tgcagtaggc gatggggcaa
    22921 acgatctgcc aatgttaaaa accgccagtt taggcgttgc tttgcacgct aaaccaaaag
    22981 tgcaagcaca agctgatttt gtggtaaact tcggggactt aacggcatta tgtttattac
    23041 ttaatgccaa tcatttattt caacattaat tgagaggaac aacaatgcca tcatttgata
    23101 ttgtatctga aatcacgatg cacgaagtgc gtaatgcggt agaaaatgcc aatcgtgtat
    23161 taagcacccg ctacgatttc cgtggggtgg aagccgttat tgagttaaat gaaaaaaatg
    23221 aaagcattaa gctcacgacc gaatcggatt tccaattaga gcagttaatt gagattttaa
    23281 tcggctcttg cgtaaaacgt gggatcgagc atagttcatt agatatccct acagaaagtg
    23341 agcatcacgg taaactctat tccaaagaaa ttaagctcaa gcaagggatt gagtcagata
    23401 tggcgaagaa aattaccaag ctgattaagg attctaaaat caaggttcaa gcccaaattc
    23461 agggtgaaca agtgcgtgtg accggtaaat ccagagatga tttacaagcc gcaattcagc
    23521 tagtgaaagg ggcagattta gggcagccat tccaatttaa taatttccgt gattagcaat
    23581 tcctgcaatt aatgatgaca agtggaaaaa ttcagcaaaa tttgaccgct tgcattaata
    23641 attcaacata aaaggaagag atgatgagcg agcaaacaga aaagaaaggc tggtttaaaa
    23701 aagcacttga gaaatatgat gccttttgcg atgagctagg ggtaaatcaa ggtgcttgcc
    23761 gaggttgtgt ccctgtggtg aagtttgacc ctgaaccgga aaaagagcag ccagaaaaat
    23821 cagaaaataa aacatagaaa aaatgcacaa tgggaagata cattgtgcat ttttttatgg
    23881 ggtttttaat tggcgtgatg atgggcaaaa tattgcttaa ctccgccatc tttcttgcag
    23941 ggattctgaa tatcagtaga gtaactaccg ttaaaacaaa gagcagtagc attcgatggt
    24001 ttaatctttt cctgtttagt tagggctggc ggttgagtat tggtttcact aatatagatc
    24061 gttttaggtt gagaatagtc tatatttttg tcgccattta cgacttgaac gggggtgttc
    24121 gatctgcaag ctacaaccga tgctgaagcc aatgtaagta taatgatatt gtgttttttc
    24181 atgggtaatt tccgttttta tatttttaat tgagagaatg ttaaaaaggg ctgatttctc
    24241 aacccttaaa gttatgcctt aagcattttt attaaaagtt tgtcctaact gaatttcatc
    24301 atttaataca ggaattaact gctctagttg atctttttca aattcgctga gattgccgat
    24361 aggtagaact ttttccacac catttgtacc taaacgaatc gggtaagcaa agaatgccgg
    24421 agaggtttcg ccattggcat tatctacata agcataacga atgacatctt ccccgattaa
    24481 ggctctcact aaaccaagtg caaattctgc tcctgcttga gccatagata aggttgctga
    24541 gccaccacct gcttttgcct caaccacttc agtaccggcg ttttggatac gatgagttaa
    24601 ttgctcgatt tgttcttgag tgaactcaag ttttaagcca tttactgttg cttgtgaaag
    24661 cagtggaaga atagttgtgc cggaatgtcc gccaatcaca ggcactctta catatttcac
    24721 attcacgtct ttcgcttcag ccacaaaggt attcgctcga ataacatcta atgttgtaac
    24781 accgaaaagt ttatttttgt catacacacc ggcttttttc aatacttctg ccgcaatcgg
    24841 aataatggtg tttaccgggt tagtaataat tgcaatgcaa gctttagggc aaactttcgc
    24901 tgctttttcg actaagttat gcacgatagt ggcattcgta ttgaataaat cggcacgtgt
    24961 catccccggc ttacgggcaa caccagcaga aataacgacc acatcggcat cttttagtgc
    25021 ctcagtcgga tcttcacctg caaaaccggt tacttttaca tcagttggaa tatggcttaa
    25081 atccactgca attcctggag taacagggga aatatcgtaa agagataatt cagatttagc
    25141 cggtaaattg agttttaata ataaggcgag tgattgaccg attccacctg cagcacctaa
    25201 aagggcaact tttttacttt gacacataaa atttctccta aatgttatgg aagtttagac
    25261 caatttagtc taagccaatt ttttgggcga ttcaaacggc aaatctcatt tttgagacat
    25321 agagcaaatt tttagctgat ttttatgggt tttattgatt tatttgcata aataagcaag
    25381 aattatgaat atttttaagt ttttgtgaat aagagtgtga aagtggacaa gatagatagt
    25441 ttatcagaag cgtttaaatc gctgttaaag gaagaaaaat tcggctcgca aagtgaaatt
    25501 gttgccgcac tacaagagat gggctttgat aatatcaacc aatccaaagt ttctcgaatg
    25561 ttatcaaaat ttggtgcagt cagaacccgt aatcctaaaa tggaaatggt gtatcagcta
    25621 ccagtcgaat taagtgtacc gactacctcc agcccactta aaaatttggt ggtagatatt
    25681 gatcataatg atgcgttaat tgtggtcaaa accagccccg gtgcggctca actgattgca
    25741 cgtttgctcg attcaatggg taaagccgaa ggtattttag gcactattgc tggcgatgat
    25801 acgattttta taacgccggt acgcaatatg caaattgaaa aattggtgaa gaatgtgaca
    25861 cagttatttg aaacatcatt atagaataag taaaggagga cttaatgttc tccttatttt
    25921 ttaggagatt attatgcaca tttttataac aggtggtacg ggttttattg ggcgtgaatt
    25981 aatccagcaa ttaaaatctg aaaatcacca ctttacactt cttactagag atacagaagc
    26041 gaaaagtggt gatttacaga ttaattattg ctattcactt gatgaacttg ttgatttaaa
    26101 tgattttgat gcagtaatta accttgccgg tgagccgatt tttgataaac gttggacaga
    26161 cgaacaaaaa cagaaattga ttgaaagccg ggttaaccta acgaataagc tcagtcaatt
    26221 aatgaatgca agctctaacc ctccgcatac ttttctctcc ggttcggcaa tcgggattta
    26281 tggcgatttg cctccttctg caaattttta tacagaatcg actgcttgtt atcaagattg
    26341 ctttaccaac cggctttgcc aacagtggga agccgctgca ctaaaagccg caagtgataa
    26401 aacccgagtg tgtttaattc gtaccggcac ggtattttcg cccgaaggcg gagcattaaa
    26461 gaaaatttta ccgctttatc gatgcggatt aggcggaaaa cttggttcag gcaaacaata
    26521 ttgggcgtgg atcagccgcc gggatcacgt acaagcggtc atttttctgc tcaaaaatgc
    26581 aaattgttcg ggtgcttata attttgttgc accaaatccg gtgagaaatg aagaatttaa
    26641 tcgtatttta gcgaatgctg ttaatcgccc ggcattcttt actgtgcctg aatttgcctt
    26701 gaaattaggc ttaggcgaac gagcttgttt agtcttagat aatcaacagc tcattccgga
    26761 aaaactactc gcagccagtt ttgtattcca agatccgatt ttaaccgaga acgttttctt
    26821 aaacccataa tttaggctat aatgctgtat aaaagcacag attaaaaggt gattatggat
    26881 ttctcattat tattagacgg tttaaatgac aagcagaggg aggcggttgc tgccccgatt
    26941 ggtaattatt tagtgttagc cggggcgggt tcgggcaaaa ctcgggtttt aacccatcgc
    27001 attgcgtggc tgattggggt ggaaaatgtg ccggaatcca atattttggc tgttaccttt
    27061 accaataaag cggcggcgga aatgcgacat cggattgagt acactctttc ccaatcgggc
    27121 gataatcgat tgttcggaat gtgggttggt actttccaca gcattgccaa ccgcttactt
    27181 cgttcccact atttagatgc ggatttaccg caagatttcc aaattatgga taccgaagat
    27241 cagcaacgtt tactcaaacg tttactgaaa ctgcataata ttgatgagaa acattttccg
    27301 ccaaaacacg ttgcgtggta tatcaacgcc caaaaagata aaggcaaacg ggcaaaagat
    27361 attgatcacc ataacgatcc gaatgaaaaa aaattggtgg agatttacca aatctatcaa
    27421 gatgcctgcg accgagccgg tttgttagat tttgccgagc tgttgattcg ggcttatgaa
    27481 ctattcaaaa ccaaaccaac cattttgcaa cgttatcaac aacgttttca gcaaattttg
    27541 attgatgagt ttcaagacac caacaatatt cagtacgact taattcgttt gcttgccggc
    27601 gatgtcggca aagtgatgat tgtcggcgat gatgatcagt ctatttatgg ttggcgtggg
    27661 gcacaggttg agaatatcca acgtttctta aatgattatg acaacgctca aaccattcgc
    27721 ttagagcaaa attaccgctc taccggcaac attttaagct gtgccaatga attaattgcc
    27781 aataacgata accgcttagg caaaaattta tggacagata gcggtgatgg cgagcctgtg
    27841 gatatttact gtgccttcaa cgaacttgat gaagctcgct ttgttgcctc gcaaatcaag
    27901 caatggaaag aagacgaggg tgatttggcg gaatgtgcgg tgttgtatcg cagcaacagc
    27961 caatctcgtg tgattgagga agctttaatt caagcgaata ttccttatcg catttacggt
    28021 ggaatgcgat tctttgaacg ccaagaaatt aaagatgctc tggcttattt acgcttaatc
    28081 gccaatcgtc aagacgatgc ggcgtttgaa cgggtgatca acacgccaac cagaggcatt
    28141 ggcgatcgta ccttagacac actccgtcaa attacccgtg gtcgccaaat tacgctgtgg
    28201 caagcaattc aagtggcggt gcaagaagaa caactttcag ggcgtgccac ttcttcctta
    28261 ttgcgttttg tagagctgat taatgctctt gatcaagaaa ccgagcagat gtcgcttgcc
    28321 gaacaaaccg attttgtgat taaaaaatcg ggtttatatg aaatgtataa gcaagaaaaa
    28381 ggcgaaaagg gcgaagttcg gattgaaaac ttagaagagt tagttaccgc aactaagcag
    28441 tttgtgaaac ctgacgaagc ggaagagctg accgatctta ccgcattttt aacccacgcc
    28501 tcacttgagg ctggagaagc ccaagcctcg caacacgaag attatgtcga actgatgacg
    28561 ctacattccg ccaaaggatt ggagtttccc cgtgtgttta ttgtgggcgt ggaagaaggg
    28621 attttcccaa gcggaatgag ctttgatgaa ggcaggttgc aagaagaacg ccgtttggct
    28681 tatgtcggca taacccgagc taagaaaaaa ctgacgatta gctatgccga atcaagacgg
    28741 ctttatggta aagaagaacg ccacgttccg tctcgcttta ttgctgaatt acctgaagag
    28801 catattcgag aagtgagatt gagaggctca attaaccgag cggcaagttt taaccaagct
    28861 aattcatttg caaaaaatcc gacaaaaacg accgcttcct ttttaaacga tgatacttgg
    28921 aaaatggggc agaaagtgaa ccacgctaaa tttggacaag gcacgattat taatgtggag
    28981 ggctcaggtg aagccacccg tttgcaagtt gcctttgtcg gcaacggcat taaatggctg
    29041 attgccaaaa tggcaaattt agagaggatt taggcgaata aaaagttttt tgcaggaata
    29101 tagatataag gggcgagata tattgcccct taaccttaaa ctaagggtaa ataaaattaa
    29161 ataatgcaag ctagatataa tgtaaagtat cagaaataag atattaattt aagataaaga
    29221 atagatggct ggggtactag gattcgaacc tagggatgcc gagatcaa
//
LOCUS       DANKBV010000014        28930 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.014, whole genome shotgun sequence.
ACCESSION   DANKBV010000014 DANKBV010000000
VERSION     DANKBV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28930
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.014"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..886
                     /locus_tag="PWY43_000500"
     CDS             <1..886
                     /locus_tag="PWY43_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL2135409.1"
                     /translation="LVEFDSSVEIANLRASEAQLPNAKANLERYRNLIASNSASKAEF
                     DNAQSTYNQLLANIEALRATIKRRQIYAPFSGLAGIVNVNVGQYITMGTEIVRVEDQS
                     SMKVRFTLPQTNLEQISIGQKVTAVIDALPAQTFPAKIVAIDPAVDRLTGLINVEAVI
                     EEGQEKLLSGMFARLNVALPTETGQIVVPQISIAYTMYGETVYVLQPLSDEDKEMVNK
                     MAVQNPSLDVNKMYRAKQMEVKTADRKGNYSQLSKGVKAGDLIVTGGLQRLSNNALVK
                     VSDTEAVGITTPAKNSKL"
     gene            900..4067
                     /locus_tag="PWY43_000501"
     CDS             900..4067
                     /locus_tag="PWY43_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL2135410.1"
                     /translation="MKFTDIFIKRPVLAVCISLLITILGLQAIEKLQVREYPEMTVSI
                     VTVSVNYSGADARLMQTLVTSQLEEAVAQADNIDYMTSSSSPSTTTVTAKMKLNTDPN
                     AALSDISAKVNAVRSNLPSGIDDPSIRVSTGSNDALMYIRFVSDELNTSQVTDYINRV
                     VKPQFFTVNGVSSVDVYGSVFGLRIWLDPDKMASNNLSGAAVLSALSANNLQTAAGNS
                     NGYYTVYRNKVLSTTPSVTELEEVTVSTTKDGKTIKLKDIATVELDKYQDAARATVGG
                     KDAVVLAVSVAATANSLTVAKDIYPMFEQVLKNLPSTIQGDIVYDKTIAIDSSIKEVI
                     KTIGEATVIVLVVIILFLGSLRAMIVPVITIPISLIGVLFFLQMFGFSINLLTLLALV
                     LAIGLVVDDAIVVLENVERHVKEGKTPFDAAIIGTREIALPVISMTITLGAVYSPMAL
                     MTGVTGTLFKEFALTLAGAVFISGIAALTLSPMMSSRILKEHKEGEESAFARVVHRSL
                     DKMTSCYTNMLSGIMAVRGFVLFFAVLIFASLPMLFGSLSSEVAPTEDRGFVVGISNG
                     PSNTNLDYTEAALAQFNQEVTKLPEVSSLMTVSGFNGANSALSIIALKDWNDRSRERA
                     AISNDVAAIGKKVVGMDVNAIAFPEISTGENGLPFSLVITTADSYEKLAIVASEFLKK
                     AQTSGQFFFANLDLKFDTVTMNMKFDREKMGAYGVTMQQVSGTLGAFLSGAIITRVDI
                     DSRAYPIVSQAVRNDRLNPEDLMNYYVTTATGQSIPLGSFVTMELKTSPSSLPRMSQL
                     NSATIGGVVSGSIGDAVNWAKAELDRTLPSGYQYDFRGESRQFVQEGNAMAMTFALAV
                     IIIYLVLAIQFESWRDPLVILVSVPLAVSGALLVMNIPTAMKGLFMGIAAGTGSKDMM
                     AFANGIWTAGYTLNIYSQVGLITLVGLITKHGILMCEVAKEEQLLHGKSRYEAIFHAA
                     TIRLRPIMMTTAAMIAGLIPLLFAVGAGAIARFSMGMVIVAGLAIGTLFTLFILPVIY
                     TFLGQEHKPLREFDEEKYFPTTKEA"
     gene            4295..5758
                     /gene="guaB"
                     /locus_tag="PWY43_000502"
     CDS             4295..5758
                     /gene="guaB"
                     /locus_tag="PWY43_000502"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL2135411.1"
                     /translation="MLRIKQEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTETKLAISLAQEGGIGFIHKNMSIERQADRVRKVKKFESGIVSEPVTISP
                     DMTLAELAELVKKNGFAGYPVIDENQNLVGIITGRDTRFVTDLSKTVREFMTPKDRLV
                     TVKENASREEIFHLMHEHRVEKVLVVNNEFQLKGMITLKDYQKAESKPNACKDEFGRL
                     RVGAAVGAGPGNEERIDALVKAGVDVLLIDSSHGHSEGVLQRVRETRAKYPDLPIVAG
                     NIATAEGAIALADAGASAVKVGIGPGSICTTRIVTGVGVPQITAIAEAAAALKDRGIP
                     VIADGGIRYSGDISKAIAAGASCVMVGSMFAGTEEAPGEIELYQGRAFKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRIAYKGYLKEIIHQQMGGLRSCMGLTGCATI
                     EELRTKAEFVRISGAGIKESHVHDVTITKEAPNYRMG"
     gene            5879..6550
                     /locus_tag="PWY43_000503"
     CDS             5879..6550
                     /locus_tag="PWY43_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2135412.1"
                     /translation="MLQAIIFDMDGVIVDTEYLEFSLQKQFIEDIKEHSRPITLEQQS
                     EVVGKCLKEIPVIIKKLSESSLPIEEIRARYYAFFQDLFSKVDFKTIFRADIQQIIQF
                     AKQNQIKLAVASSSALSHIENILTVCGIKDEFDLIVSGEQFEHSKPDPTIYRYTCEKL
                     GVEPQNAVAIEDSYYGMLAAKTAGLTVIGYEEKRILIDQSLADYMGKDMKEILKILQK
                     CNTPS"
     gene            6672..6983
                     /locus_tag="PWY43_000504"
     CDS             6672..6983
                     /locus_tag="PWY43_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135413.1"
                     /translation="MKKLSLISIVALSACSIFGPSYSGETTASPLLKLDTERNINLYF
                     QAIHKCTPDKIHTKIINIPSEGNAKELWTATGCNKTQSFIITYRNDGVGGTFINMTTT
                     P"
     gene            7000..8571
                     /gene="guaA"
                     /locus_tag="PWY43_000505"
     CDS             7000..8571
                     /gene="guaA"
                     /locus_tag="PWY43_000505"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2135414.1"
                     /translation="MTNIHHHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQ
                     IREFNPTGIILSGGPESTTEENSPRAPEYVFNAGLPVLGICYGMQTMAMQLGGLTETS
                     DHREFGYASVAIKSADQLFANLNDDLTACEPKLDVWMSHGDKVTKLPPHFTITGVTPT
                     CPIAAMSDESRRFYGVQFHPEVTHTKQGLALLKNFVVGICGCETNWTAENIIEDAVKR
                     IREQVGDDEVILGLSGGVDSSVTALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFG
                     NKFGLNIVRVNAEDRFLEALKGLDEPEAKRKTIGKVFVDVFDDESKKLSSVKWLAQGT
                     IYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLP
                     AEMLNRHPFPGPGLGVRVLGEIKKEYCDLLRKVDAIFIEELHKAGWYYKVSQAFTVFL
                     PVKSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDLLGKISNRIINEVNGISRV
                     VYDVSGKPPATIEWE"
     gene            complement(8732..9127)
                     /locus_tag="PWY43_000506"
     CDS             complement(8732..9127)
                     /locus_tag="PWY43_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcB family protein"
                     /protein_id="HDL2135415.1"
                     /translation="MEQWTTDVWAAIMAAFIVGAVIGCTLVRVFNSSVRKQHELEKEL
                     KQTKTQVDEQKQKLEQHFEQSAQLLSSLASDYKKLYTHLAQSSEKLLPEASKNITFFQ
                     QTKLPIPAEKTEEDQPRDYSEGSSGLLKS"
     gene            9426..9854
                     /gene="rplM"
                     /locus_tag="PWY43_000507"
     CDS             9426..9854
                     /gene="rplM"
                     /locus_tag="PWY43_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL2135416.1"
                     /translation="MKTFVAKPETVQRDWYVVDATGKTLGRLATELARRLRGKHKPEY
                     TPHVDTGDYIIVINADKVAVTGKKQTDKIYYWHTGYVGGIKDATFKEMIARRPEAVIE
                     IAVKGMLPKGPLGREMFRKLKVYAGSEHNHQAQQPQVLDI"
     gene            9871..10266
                     /gene="rpsI"
                     /locus_tag="PWY43_000508"
     CDS             9871..10266
                     /gene="rpsI"
                     /locus_tag="PWY43_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2135417.1"
                     /translation="MTAANQNYGTGRRKSSSARVFIKPGSGNITINQRSLEVYFGRET
                     ARMIVRQPLELVELTDKLDLYITVKGGGISGQAGAIRHGITRALMEYDETLRPALRAA
                     GFVTRDARRVERKKVGLHKARRRPQYSKR"
     gene            10366..11142
                     /gene="yaaA"
                     /locus_tag="PWY43_000509"
     CDS             10366..11142
                     /gene="yaaA"
                     /locus_tag="PWY43_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDL2135418.1"
                     /translation="MLAIISPAKTLDFKTNIDGFAFSQPNLTACSQELIDICKQLSPA
                     EVGSLMSISDKLAALNVARFAEWQLEHNQQNSKAAIFAFKGDVYTGLDAETLTKEQVE
                     YAQQHLRMLSGLYGLLKPLDLMQPYRLEMGTKLANPKGRDLYAFWGNIITEHLQQAID
                     EQGDDILINLASDEYYGAVKPQNLNATIIKPVFLDEKNGKYKVISFYAKKARGMMVRF
                     MLDTQPTSIEQLKQFNYGGYWLDADSSSENELVFKREEQA"
     gene            11142..11705
                     /locus_tag="PWY43_000510"
     CDS             11142..11705
                     /locus_tag="PWY43_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HDL2135419.1"
                     /translation="MSATKKGITLVALSLAGCVSQSPQFAVPDAIRFGQDTFVKVTHS
                     QIDEMQHLLYLPEKSEKNLENWQKGILFFLDKNSKNQTLEQRAAFRQASFAKKPELEA
                     KVAIEQNELHSSVIYPPTERFNNVMLEVTRGRNLACGYGQIQFADKREVSKAIAKKLP
                     NLTAYSQEIAKLSLELNQLGWQVQCSK"
     gene            11714..12613
                     /locus_tag="PWY43_000511"
     CDS             11714..12613
                     /locus_tag="PWY43_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL2135420.1"
                     /translation="MNEKYSKLVKSAANLAIIVAILLIVIKAFAWWKTGAISILAAMT
                     DSVVDLFASLTNILVLRFALQPADENHAFGHGKAESLAALAQSAFISGSALFLLLQGF
                     HKLTNPELIAQSDIGVIVSIASIVITALLVFYQRYVVTLTKSPAIEADSLHYQTDLLM
                     NAAILVAMLLNSAGVVYADAVFAIGIALYIAFNAVKMFWCAVQTLLDQALPDEEIEQI
                     KQIASQHPNILGIHDILTRRAGAVRFIQLHLELDDHLTLLVAHEITDSLEQKLLAAFP
                     MSHVIIHQEPTTVVKQEFMAENI"
     gene            12613..13365
                     /locus_tag="PWY43_000512"
     CDS             12613..13365
                     /locus_tag="PWY43_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon-containing protein"
                     /protein_id="HDL2135421.1"
                     /translation="MYLAIVHFSYHNFEQDPVTAISQVLNQWLYNGQVIGREMPITFH
                     QNEFQARVCVPTQESLLPQYNSDEVNEALQQAVEIGVNFVSFELVGRDYQGEESSHNR
                     SPAFQILYTTHLDTCSPLYDGEQFAPIPLYSLQDQSLSEAILEWQQNWQACDLLQMNG
                     NVLECHALAQISDETSVLATIGRDLCQQIEQRTHIPTFYYLYRLGRDEGEYQRKCPCC
                     GGEWKLTEPLHDIFHFRCDPCRLISNLSWELL"
     gene            13466..14790
                     /locus_tag="PWY43_000513"
                     /pseudo
     CDS             13466..14790
                     /locus_tag="PWY43_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
     gene            complement(14850..15509)
                     /locus_tag="PWY43_000514"
     CDS             complement(14850..15509)
                     /locus_tag="PWY43_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2135422.1"
                     /translation="MFNISKQQVLDAFHFRSACRSYDPNKKINKEDMDYILELGRLSP
                     SSVGSEPWKFLVLQNPETRQKIAPVSWGIKHPMEEMSHLVVILTKKNVRYDSVYMRDM
                     LAKRGLTYEQIEASLVRYKSFQEEDMKLLSERELFDWSTKQTYIALGNMMTGAALIGI
                     DSCPIEGFHYDTVNQILSDEGLFDLNEYGVSCMITFGYRNKEIKKKSRKPTEEVIAWI
                     E"
     gene            15738..16724
                     /gene="pheS"
                     /locus_tag="PWY43_000515"
     CDS             15738..16724
                     /gene="pheS"
                     /locus_tag="PWY43_000515"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL2135423.1"
                     /translation="MQHLKDLTNKAREALDKIDQNLEALEEFRVEYFGKKGHFTTLMQ
                     ELRNIAAEERPAIGAKINEAKQVVLDVLNAKKEEWEQAVLNAKLANESIDVSLPGRKT
                     ELGGLHPVSITIERVVKFFSELGFTVANGPEIETDYYNFDALNIPDHHPARADHDTFW
                     FDANRLLRTQTSGVQIRTMENVKPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKK
                     ANFTELKGLIHDFLKAFFEEDLQVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMV
                     HPNVLRNVGIDPEEYSGFAVGMGVERLTMLRYNVTDLRSFFENDLRFLKQFK"
     gene            16772..19159
                     /gene="pheT"
                     /locus_tag="PWY43_000516"
     CDS             16772..19159
                     /gene="pheT"
                     /locus_tag="PWY43_000516"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL2135424.1"
                     /translation="MKFNESWLREWVNPAISTEQLCDQITMLGLEVDDVEPVAGAFSG
                     VVVGEVVECAQHPDADKLRVTKVNVGGERLLDIVCGAPNCRQGLKVACAVEGAVLPGD
                     FKIKKTKLRGQPSEGMLCSYSELGIKEDHSGIIELPADAPIGQDFREYLNLNDVAIEI
                     SLTPNRADCLSIAGIAREVGVINRVDVKAPTITDVKATISDKVSVELQAPEACPRYLA
                     RVVKNVNVKATSPLWLQEKLRRCGIRSIDPIVDITNLSLLELGQPMHAFDAAKIEGGI
                     QVRMAKEGEELVLLDGTNAKLQSNTLVIADSKGALAMAGIFGGEASGVSENTKDVVLE
                     SAFFASLAITGRARQYGLHTDASHRFERGVDPELTRQAMERATALLLEICGGEASEIV
                     EAVSQENLPKRNTVTLRRSKLDSVIGHHIDDATVTDILTRLGLNVTFADNSWTAIAPS
                     WRFDIEIEEDLIEEIARIYGYNSIPNNSPLAHLTMKGTPEKLLEANRIRMALVDSDYQ
                     EVITYSFVDPKKQSLLHPQQEALILPNPISSEMSAMRVSLLTGLLDTIVYNQSRQQNR
                     VRIFEAGLRFIPDSNAESGVRQEFVFGAAIVGDKRPVHWENKSEAADFFDLKGDIERI
                     LSLTAIRHDLQFVAKQFPALHPGQSAAIMLDGKEIGFIGTVHPSIVQKFGIKGKPIVC
                     EILASAIAERPVTQAGEISKFPANNRDIAVVVDESVPAGEVLEACRKAGGNKLVAVNL
                     FDVYRGSNLESGKKSLAISLTVQDNEKTLEENEINAVIQAVLAELATRFNAVLRD"
     gene            19217..19513
                     /locus_tag="PWY43_000517"
     CDS             19217..19513
                     /locus_tag="PWY43_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDL2135425.1"
                     /translation="MALTKIEIAENLIEKFGLEKRVAKQFVELFFEEIRSSLENGEEV
                     KLSGFGNFSLREKKARPGRNPKTGENVAVSARRVVVFKAGQKLRERVEQASSQS"
     gene            19552..20073
                     /locus_tag="PWY43_000518"
     CDS             19552..20073
                     /locus_tag="PWY43_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2135426.1"
                     /translation="MKLTKRWLSLGCLLATTAMLVACSSSDTTSKSQVKSKVYRSIYQ
                     SDLSDPIMAISRLSEHQHEWKGTRYRLGGNSKAGIDCSGFMQVTFKDLFGIDLPRTTT
                     EQAKAGERISKDELRTGDLVFFKTGRGPNGKHVGVYVKNGQFLHASTKGGVIYSDLDS
                     PYWSKAFWQARRL"
     gene            20089..21132
                     /gene="degS"
                     /locus_tag="PWY43_000519"
     CDS             20089..21132
                     /gene="degS"
                     /locus_tag="PWY43_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDL2135427.1"
                     /translation="MLKKIGQAVIFGLFCAILILFVAPIIQSKGSSFSLSAFTQKEIV
                     SYNAAVKIASPAVVNVYSRNFSSENETFEVKNLGSGVIMSSDGYILTNRHVIEDADQI
                     VIALQNGAISTNVKLVGDDSLTDLAVLKIEGENLPTIPQNPERKLQIGDVVLAIGNPL
                     NLGQSITQGIVSAIGRKTLTESGRQNFIQTDVSINQGNSGGALINTAGELIGINTLRF
                     GKNTNELSEGLNFAIPINLANQIMSKIIKDGRVIRGYFGVSTDLLDTAKMQGYEKGVI
                     IVNVDKNGPAAKAGIQAGDLVLKVGDIEAFSPSQMMEELAEMKPYTTVKVLVQRDEQL
                     LNFDVTITELQTQ"
     gene            21141..21815
                     /gene="cmk"
                     /locus_tag="PWY43_000520"
     CDS             21141..21815
                     /gene="cmk"
                     /locus_tag="PWY43_000520"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL2135428.1"
                     /translation="MKKIVITVDGPSGAGKGTLCHALAEKLGFDFLDSGALYRITALS
                     AIKKEVPLDDEEKLAEVGRNLDIQFLPENGEIQVILEGENVGDLIRTAEAGQNASKVA
                     AFPLVREALLERQRAFSSEKGLIADGRDMGTVVFPDAQIKLFLDASAEERTKRRVKQL
                     QEKGFNANFDEILAEIKERDFRDRNRAVAPLVPAKDALLLDSTHLSIEDVINQALNHI
                     AQYIKF"
     gene            21907..23574
                     /gene="rpsA"
                     /locus_tag="PWY43_000521"
     CDS             21907..23574
                     /gene="rpsA"
                     /locus_tag="PWY43_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL2135429.1"
                     /translation="MSELSFAQLLEESFAATPRLGDVVKGTVVAIQKGLVIVDTGFKS
                     ESAIDASEFTNAQGELEVQVGDEVDVVLKAVEDGFGATVVSRGDAKRNEAWIALEKAF
                     EEQATVIGFVNGKVKGGFTVELNGVRAFLPGSLVDTRPVRDSLNLEGKEYEFKVIKLD
                     QKRNNVVVSRRAVIETESNQDRDAVLANLVEGSEVKGTVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEVVNVGDEVTVKVLKFDKDKTRVSLGLKQLGQDPWAVIAENHPV
                     NSKLTGKVTNLTDYGCFVEILDGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLE
                     VDEERRRISLGLKQCKANPWEQFAATHSKNDKVSGKIKSITDFGIFIGLEGGIDGLVH
                     LSDISWNVSGEEAVRNYKKGDEVEAVVLQVDAVKERISLGIKQLESDPFTNFVDGTKK
                     GAILTGKVVEVDAKGVKVELEGGVEAFIRANEATAERVEDITSVISVGDSIEAKYTGV
                     DRKSRVVNLSVRAKNEAEESAAIAQVNKEEVVVPNAFAEAFKAAKGE"
     gene            23703..23984
                     /locus_tag="PWY43_000522"
     CDS             23703..23984
                     /locus_tag="PWY43_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDL2135430.1"
                     /translation="MTKSELIESLASKNPSLPIKMVEHCVKELLEQLTATLEEGERIE
                     VRGFGSFSLHYRQPRLGRNPKTGESVLLGAKYVPHFKAGKDLKERVDLL"
     gene            24021..24308
                     /locus_tag="PWY43_000523"
     CDS             24021..24308
                     /locus_tag="PWY43_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL2135431.1"
                     /translation="MIKYILGILIVIAVIIVAVTIGANNDQLITFNYIFAQSELRLST
                     LVAILFGLGLILGWLVTGIFYLKIKFQNVALNRRVKRQAQQITELTTPKAE"
     gene            24312..25508
                     /gene="lapB"
                     /locus_tag="PWY43_000524"
     CDS             24312..25508
                     /gene="lapB"
                     /locus_tag="PWY43_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDL2135432.1"
                     /translation="MLELLFLLLPIAALYGWYMGQRSAKKDQETASNKFSREYVTGLN
                     FLLSNQQEKAVDLFLSMLQKQESENHIASESQFEAELTLGNLFRSRGEVDRALRIHQT
                     LVNSPHYSFDQKLLAKQQLAKDFMVAGFYDRAENYYISLVDEPEFAKYSLSQLAVIYQ
                     KTREWKKAINVTEKRLRIEPDADKIPLSHFYCEYAQGVKFDDKAAFIESLNKALEYYP
                     QSTRASIILGDFYLEEGEYLRALSYFEQILVQDPDYISEVLGRIKQSYMALEDLNGYE
                     LFLIRANRVKHNSSVDIALTEFIEEKDGINAAHSKLYQQLSTYPNLITFHRFIRYQAD
                     FAEEGNGKESLVLLHNMVGNQIKRSFQYRCLNCGYQSYRLMWQCPSCNQWEKIKPVQS
                     IEGIIQ"
     gene            25532..26224
                     /gene="pyrF"
                     /locus_tag="PWY43_000525"
     CDS             25532..26224
                     /gene="pyrF"
                     /locus_tag="PWY43_000525"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL2135433.1"
                     /translation="MNNKVIVALDYETEREALEFVDLVDPSLCRLKVGKEMFTTLGTN
                     FVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDLHASGGLAMMEEAKKIL
                     EPYGKEAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
                     VEVLRTHCGQDFKLVTPGIRPKGTDFGDQRRVMTPKQAVETGSDFLVIGRPITQAQDP
                     LAVLKSINQSIS"
     gene            26307..26618
                     /gene="yciH"
                     /locus_tag="PWY43_000526"
     CDS             26307..26618
                     /gene="yciH"
                     /locus_tag="PWY43_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDL2135434.1"
                     /translation="MTLVYSTETGRIKSEVIKEERPKGDGIVRIQRQTSGRKGKGVCL
                     ITGLDLEDKALNSLASELKRKLGCGGSFKDGVIEIQGDNRGFIKQILEQKGFKVKLAG
                     G"
     gene            26721..28883
                     /locus_tag="PWY43_000527"
     CDS             26721..28883
                     /locus_tag="PWY43_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDL2135435.1"
                     /translation="MWKKTLIAVFLASQYAFAVGTGKEPSKSYTETELKQLKNDWLAI
                     YQKELQYAENLRLKQRENFQQINYLADAAISQKNLSLSTEQIIQRLLLTLAGYPLEMK
                     AEWKLLNAKITLKQATESEVKAFISKYPDSLYQRQLQNLMFELLYREQKFPELMEYAQ
                     NVKPSSLADQCRLFSARYELAASKAQINPEVSQVTEQKAENAELLALVQEFDRFWLAT
                     PKLTSDCANLESYWRDQGFKTDEKVRLKAVELIKQNANAELANLAVNVNNENLKIWLS
                     SVEKVANNPADLQNFIENQPLDSPFYAENKAIIQQLFAKYVRTLSENMENPSFQQYQA
                     WAEKYQFSNEEMNSWKNTFITRLFDNPEPTFQLWRDEQIKLLNTDNLTERRLRMAIWQ
                     QTALNEWLDLLSTEAKGKTEWRYWAAKEEKNEAKRKADFENLAKERGFYAMLAAQQLG
                     IIYQIPMLEVPELTQTQLELYENQLARIQELRELGQFEQARRVWIDWLKNIKAEAGEN
                     TPTKIQLALIKYAKDKNWYDLAVEGTIQIKAFDYLDLRLPNAYSDWFDLHLQDKKISK
                     TFAQAIARQESAWNAQAQSHANARGLMQLLPTTAQKTAKDNGLTFKDGNDLFQPFNNI
                     MLGTTHLMELNEKYPNNRILIASAYNAGAGRVEQWLKRSNGQLAMDEFIASIPFYETR
                     GYVQNVLAYDYYYQMRYSDINDKNGLKMFYQEELTRKY"
BASE COUNT         8823 a         5188 c         6463 g         8456 t
ORIGIN      
        1 gttagtagaa tttgacagca gtgttgaaat tgctaacttg agagcctctg aagcgcaatt
       61 accgaatgcc aaagcaaatt tagagcgtta ccgtaatctg attgcctcaa acagtgcttc
      121 taaagccgag tttgataatg ctcaatctac ctacaaccaa ttattagcca atatcgaggc
      181 attgcgtgct acgattaaac gccgtcaaat ttatgctccg tttagtggtt tagcgggtat
      241 cgtgaatgtg aatgttgggc aatacattac aatgggtacg gaaattgtgc gtgtggaaga
      301 tcaatcttca atgaaagtac gttttacctt gccgcaaact aatttagagc aaatctcgat
      361 tgggcaaaaa gtgacagccg taattgatgc gttaccggca caaaccttcc cagctaaaat
      421 tgtggctatt gatccggctg tggatcgttt aaccggctta attaatgtgg aagcggtgat
      481 tgaagaaggg caggaaaaat tgctttcagg tatgtttgcc cgcttaaatg ttgctttacc
      541 aacagaaacc ggacaaatcg tcgtaccaca aatttctatc gcttacacta tgtatggtga
      601 aacggtatat gtgctgcaac cattatctga tgaagataaa gaaatggtga acaaaatggc
      661 ggtacaaaac ccaagtttag atgtaaacaa aatgtaccgt gcaaaacaga tggaagtgaa
      721 aacggctgac cgcaaaggaa actattcaca attatccaaa ggcgtgaaag caggtgatct
      781 gattgtaaca ggcggtttac aacgtttaag taataatgcg ttggttaaag tttctgatac
      841 agaagccgta ggcattacca caccggcgaa aaacagtaaa ctgtagtgga gtgagttaag
      901 tgaaatttac tgacatcttt attaaacgtc ctgttttggc agtttgtatc agcctattga
      961 ttacgatcct aggcttgcaa gcaatcgaaa aattgcaggt gcgtgaatat ccggagatga
     1021 cggtctcgat tgtaaccgtg agtgttaact attcaggtgc agatgcacgt ttaatgcaga
     1081 cactggttac ctcacagtta gaagaggctg ttgctcaagc ggataatatt gactatatga
     1141 cttcatcaag ctctccaagt accactacgg taacggcaaa aatgaagtta aataccgatc
     1201 cgaatgccgc actttccgat atttcggcaa aagtgaatgc ggtacgctca aatttaccga
     1261 gcggtattga tgatccgtca attagagtct caaccggttc aaatgatgcg ttaatgtata
     1321 tccgatttgt ctctgatgaa cttaatacat cacaagttac cgattatatt aaccgtgtag
     1381 taaaaccaca gttctttacg gtaaatggtg tttctagcgt tgatgtgtat ggctctgttt
     1441 ttggcttacg catctggctt gatccggata aaatggcgag caataaccta tcaggggcgg
     1501 ctgtattatc ggcattaagt gccaataact tgcaaaccgc tgcgggtaat tctaacggtt
     1561 actatacggt ttatagaaac aaagtacttt ctaccacgcc ttctgtgaca gagttagaag
     1621 aggttacggt ttcaaccacc aaagatggaa aaaccatcaa attaaaagat attgcgactg
     1681 ttgagctgga taaataccaa gatgcagcaa gagcaacggt tggcggtaaa gatgcagttg
     1741 tgcttgctgt ttcggtagcc gcaacggcaa actctttaac cgtagcgaaa gatatttatc
     1801 cgatgtttga gcaggtatta aaaaatctac caagcaccat tcaaggagat attgtttacg
     1861 ataaaaccat tgcgattgat agttctatca aagaagtaat taaaactatt ggtgaggcaa
     1921 ccgtcattgt attggtagta attatcctgt tcttaggttc attacgagca atgattgtac
     1981 cggtgattac cattccgatt tccttaatcg gggtattatt cttcctgcaa atgtttggtt
     2041 tctcgatcaa cttgcttact ttgctggcgt tggtattagc gatcggcttg gtagtggacg
     2101 atgcgattgt cgtacttgaa aacgtagaac gtcacgttaa agaaggtaaa acgccatttg
     2161 atgcagcgat tatcggtaca cgtgaaattg ctttaccggt tatttcaatg actattactc
     2221 tgggtgcagt atattcacca atggcattaa tgaccggggt aacagggaca ctatttaaag
     2281 agttcgcttt aacgcttgcc ggtgcggtat ttatttcggg gattgcggca ttaacccttt
     2341 cgccgatgat gtctagccga atattaaaag aacataaaga aggggaagag tcagcatttg
     2401 cccgcgtggt gcatagatca ctagataaaa tgaccagctg ctataccaat atgctaagcg
     2461 gtattatggc tgttcgtggt ttcgtcttat tctttgcggt gctgatcttc gccagtttac
     2521 cgatgttatt tggctcactt tccagtgaag ttgcaccgac agaagaccgt ggctttgtgg
     2581 ttggtatttc taatggacct tcaaacacca atttagatta taccgaagca gcgttagccc
     2641 aatttaatca ggaagtaact aaattacctg aagtatcttc cttaatgaca gtttcaggct
     2701 ttaatggtgc aaatagtgca ttatcgatta ttgcattaaa agattggaat gatcgtagcc
     2761 gtgaacgtgc tgcgatttct aatgatgtgg ctgcaatcgg taaaaaagtg gtgggtatgg
     2821 acgtgaatgc aattgcattc cctgaaattt caaccggtga aaatggtcta ccattctcat
     2881 tagtgattac taccgctgat agctatgaaa aattggcaat cgttgcctct gaattcttga
     2941 aaaaagccca aacttccggt cagttcttct ttgcgaattt agatttgaaa tttgatacgg
     3001 tcacgatgaa tatgaaattc gaccgtgaaa aaatgggtgc ttatggcgtg acaatgcagc
     3061 aggtgagtgg cacattaggg gcattcttat ccggtgcgat tattactcgt gtcgatattg
     3121 actctcgtgc ttacccgatt gtgtcgcaag cggtgcgtaa tgatcgttta aatcctgaag
     3181 atttaatgaa ttactatgtc acgactgcaa cagggcaatc tatcccgtta ggttcatttg
     3241 tgacgatgga attaaaaaca tcaccatctt ctttacctcg tatgagccaa ttaaactcgg
     3301 ccaccattgg tggggtagta tctggctcaa tcggcgatgc ggtaaattgg gcgaaagcag
     3361 aattagatcg cactttacca tcaggttatc aatatgactt ccgtggtgaa tctcgccagt
     3421 tcgttcaaga aggtaatgcg atggcaatga ctttcgctct agcagttatc attatctatc
     3481 ttgtacttgc aattcaattt gaatcttggc gtgacccatt agtaattttg gtttctgtgc
     3541 cgttagctgt gagtggtgca ttgttggtaa tgaacattcc aaccgcaatg aaaggcttgt
     3601 ttatggggat tgcagcaggc actggctcga aagatatgat ggcattcgcc aacggtattt
     3661 ggacggcagg ttatacctta aatatctatt cacaagtagg tctaattacc cttgtcggct
     3721 taattactaa acacggtatc ttgatgtgtg aagtggcgaa agaagagcag ttactgcacg
     3781 gcaaatcacg ctatgaagct attttccacg cagccacgat tcgtttacgc ccaattatga
     3841 tgacaacggc agcaatgatt gcaggcttaa ttccgcttct gtttgcggtg ggtgccggtg
     3901 caatcgcccg cttcagtatg ggtatggtaa tcgtagcagg cttggcaatc gggacattgt
     3961 ttactctgtt tatcttgccg gttatctaca ctttcttagg gcaagaacac aagccattac
     4021 gtgaatttga cgaggaaaaa tatttcccaa cgacaaaaga agcatagttt taaatagcaa
     4081 aagagcgtag cgatacgctc ttttttgttg gcttacaagc ggtcgttttt tatttattgt
     4141 ttgctaaaaa atcacaaaat ttaaccgctt gaaagtatat tttcagatag cgtaaacgtt
     4201 tccctattta cagaaaccta gttcctctgt ataatccgct cgcaattttc cagtacagat
     4261 tttcaattta atcatttttt aaggaataat tgcaatgcta cgaattaaac aagaagccct
     4321 cacttttgat gatgttcttc tcgtcccggc acattctact gtgcttccta atactgctga
     4381 tctttctact caattaacta aaaccattcg tttaaacatt ccgatgcttt ctgctgcaat
     4441 ggataccgtt acagaaacta agcttgcgat ctcccttgct caagaaggcg gcattggttt
     4501 tatccataaa aatatgtcga ttgaacgcca ggcagaccgt gtgcgtaaag tgaaaaaatt
     4561 tgaaagtggt attgtttctg agccagtgac gatttctcct gatatgacat tagcggaatt
     4621 ggctgaattg gtgaaaaaga acggttttgc aggctatccg gtgattgatg aaaaccaaaa
     4681 tttagtggga attattaccg gacgtgatac ccgatttgtc acggatttaa gcaaaacagt
     4741 gcgtgaattt atgacaccaa aagaccgttt agtgacggta aaagaaaacg caagccgtga
     4801 agaaattttc cacttaatgc acgaacaccg agtggagaaa gtgctggtag tgaataatga
     4861 atttcagtta aaaggaatga ttaccctaaa agactaccaa aaagcggaaa gcaaaccgaa
     4921 tgcctgtaaa gatgagtttg ggcgtttgcg tgtgggggcg gcagtgggag ccggtccggg
     4981 caatgaagaa cgaattgatg ctttagtaaa agcgggtgtc gatgtgctat taatcgactc
     5041 ttcgcacggg cattctgaag gtgtattaca acgtgtgcgt gaaacccgtg caaaataccc
     5101 tgatttaccg attgttgccg gtaatattgc cactgcagaa ggagcgattg cgttagctga
     5161 tgcaggagcc agtgctgtga aagtaggaat cggcccgggt tcaatttgta ccaccagaat
     5221 tgtaacaggc gttggcgtgc cacaaatcac ggcaatcgca gaagcggcag ctgcgcttaa
     5281 agatcgaggc attcctgtga ttgctgatgg tggaattcgt tattcaggcg atatttcaaa
     5341 agctattgcc gccggtgcaa gttgcgtaat ggtcggttcg atgtttgccg gcacagaaga
     5401 agccccgggt gaaattgagc tttatcaagg cagagcattc aaatcctacc gtggaatggg
     5461 atcattaggt gcaatgagta aaggctcgtc agatcgctat ttccaatctg ataatgccgc
     5521 cgacaagctc gtaccggaag ggattgaagg gcgtatcgct tacaaaggct acttgaaaga
     5581 aattatccac caacaaatgg gcggtttacg ctcctgtatg ggattaaccg gctgtgccac
     5641 tattgaagaa ctccgcacca aagcagaatt tgtccgcatt agtggtgctg gtattaaaga
     5701 aagccacgtc cacgatgtga caattaccaa agaagcaccg aactaccgaa tgggttaata
     5761 gcctttctca ctctctcccc ttgtgggaga gagaccgttt taaattacgt tcagtaattt
     5821 aaaacagaga gagggaataa gtggttagat tttcttaaaa ttttacaaaa gaggctatat
     5881 gctccaagct atcatttttg atatggacgg cgtgattgtg gataccgaat atctggaatt
     5941 ttccttacaa aaacagttta ttgaagacat caaagaacac agccgcccga ttactttaga
     6001 acaacaatcg gaagtggtag gcaaatgttt aaaagaaatt ccggtgatta tcaaaaaact
     6061 aagcgaatcc agtttgccga ttgaggaaat tcgtgcacgt tactacgcct ttttccaaga
     6121 tctgtttagc aaagtggatt ttaaaaccat tttcagagcc gatattcagc aaattattca
     6181 atttgccaag cagaatcaaa ttaagcttgc ggttgcctct tcttccgccc tttcgcatat
     6241 tgaaaatatt ctcacggttt gcggcattaa agacgaattt gatttaatcg tcagcggtga
     6301 acagtttgaa cacagcaagc cagacccaac catttaccgc tacacctgcg aaaaattagg
     6361 cgttgagcca caaaatgcag tggcgattga agactcctac tacggaatgc tcgccgccaa
     6421 aaccgcaggc ttaacggtga ttggttacga agaaaaacga atactgattg accaaagttt
     6481 agcggattat atgggcaaag atatgaaaga gattttgaag attcttcaaa aatgtaatac
     6541 tccaagttaa taaatggtcg atttttatag aaattttgtc aatgtttcta cttcaagtac
     6601 ttggaacagt ctatcggctt gagtaaccta gaaatgagag tggatatttt aatattaata
     6661 ggataaatag aatgaaaaaa ttaagtttaa tttcaatcgt cgctttatct gcgtgcagta
     6721 ttttcggacc atcttattca ggagaaacaa cggccagtcc tttattaaag ttggacacag
     6781 aaagaaatat aaatctgtat tttcaagcaa tacataaatg tactcctgat aaaattcata
     6841 caaaaatcat caatattcca agtgaaggta atgcaaaaga actttggact gcaacaggtt
     6901 gtaataaaac gcaatctttt attattacat atagaaacga tggggtaggc ggcactttta
     6961 ttaacatgac cacaacgcca taatttaaag gaaattacaa tgacaaacat ccaccaccac
     7021 aaaattttaa ttttagactt cggttcacaa tatactcagc ttatcgcacg ccgtgtgcgg
     7081 gagattgggg tttactgcga gctttgggct tgggacgtaa cggaagagca gatccgtgaa
     7141 tttaacccaa cggggattat tctttcagga ggtcctgaaa gcaccactga ggaaaacagc
     7201 ccacgagcgc cggagtatgt gtttaacgct ggtctgccag tgctgggcat ttgctacgga
     7261 atgcagacaa tggcgatgca gttgggcggt ttaaccgaaa cgtccgacca ccgtgaattt
     7321 ggctatgcct cggtggctat caaatctgcc gatcaacttt ttgcaaattt aaacgatgat
     7381 ttgaccgctt gcgagccaaa attggacgtg tggatgagtc acggcgacaa agtcaccaaa
     7441 ttaccaccgc actttaccat cacaggcgtt accccaacct gcccgattgc cgcgatgtcg
     7501 gacgaaagcc gccgtttcta tggcgtgcag ttccaccccg aagtgaccca caccaaacaa
     7561 ggcttggcgt tgctgaaaaa cttcgtggtc ggcatttgcg gctgtgaaac gaactggacg
     7621 gcagaaaaca tcattgaaga tgcggtgaaa cgcattcgtg agcaagtggg cgatgacgag
     7681 gtgattttag gtttgtcggg tggggtggat tcctccgtca ccgccttgct attacatcgt
     7741 gctatcggta aaaacctgca ctgcgtattt gtggataacg gcttgctccg cttgaacgaa
     7801 ggcgatcaag tcatggaaat gttcggcaac aaattcggtt tgaacattgt gcgagtgaat
     7861 gccgaagacc gtttcttaga agcgttaaaa ggcttagacg aaccggaagc taaacgcaaa
     7921 accattggta aagtgtttgt ggacgtgttt gatgacgaaa gcaaaaaact tagctcggtc
     7981 aaatggcttg cacaaggcac gatctaccct gatgtgattg aatccgccgc cagcaaaacc
     8041 ggcaaagccc acgtgattaa atcacaccat aacgttggcg gcttgccgga ttatatgaag
     8101 ctcggcttag tcgaaccgtt gcgtgaattg tttaaagacg aagtgcgcaa aatcggcttg
     8161 gcactcggct taccggctga aatgctcaac cgccacccgt tccccggccc gggcttaggc
     8221 gtgcgtgtgc tgggcgaaat caaaaaagag tactgcgatt tactgcgtaa agtcgatgcg
     8281 atcttcatcg aagaactgca caaagccggc tggtactaca aagtcagcca agctttcacg
     8341 gtattcttgc cggtgaaatc ggtgggcgta atgggcgatg gacgcaaata tgactgggtc
     8401 gtcagcttgc gtgcggtgga aaccatcgac tttatgaccg ctcactgggc gcacttgccg
     8461 tatgatctat taggcaaaat ctccaaccgc attattaacg aagtgaacgg tatttcccgt
     8521 gtggtgtatg acgtgagcgg aaaaccaccg gcaaccattg agtgggaata actcttacaa
     8581 aataggcttc ttataaccta agttcagtat ctaaagctga aaaatgttgt taactgctta
     8641 atgtagcggt ttagaataaa ggattgattc caacggaatc aatccttaaa ctaagtgcct
     8701 tttagtaaaa ctaaatattc aatttttagt tttatgattt cagtaagcca gatgaacctt
     8761 cggaataatc tctaggttga tcttcttccg ttttttctgc cggaataggt aatttggttt
     8821 gttggaaaaa ggtaatattt tttgatgctt ctggtaatag cttttcagag ctttgtgcta
     8881 aatgggtata aagcttttta taatcagaag ctaaacttga aagtaattgg gctgattgct
     8941 caaagtgttg ttcaagtttt tgtttttgct catccacttg agttttggtt tgcttcaact
     9001 ctttttccag ttcatgctgt tttctgacac tactgttaaa tactctaact agagtgcaac
     9061 ctattacagc acctacaata aatgctgcca taatggctgc ccaaacatct gttgtccatt
     9121 gttccatcat aaactcctta tgttttagtg attgattact gctattttta acatttcagt
     9181 ttttatttga aaagctccaa atgattaaaa tgtgatatag gttcaatttt gaaagattat
     9241 cagcaaaaaa tgttagattt ttgagtgaaa tctctgtata atttcgccac cctgtttttg
     9301 tgtggttttc cccgccacac aagtttggtc tgaatcagac tcgaaggggt cgggttcacc
     9361 gtaaaataat aggaaatacc tcaatgaggt attgggtttt aaactttaaa tttggtattt
     9421 aattaatgaa aacttttgta gcaaaaccag aaacagtaca acgtgactgg tatgtagtag
     9481 atgcgacggg taaaacatta ggtcgtttag cgactgaatt ggcacgccgt cttcgtggta
     9541 aacataaacc tgagtacact ccacacgttg ataccggtga ttacatcatc gtgatcaatg
     9601 cagacaaagt agcagtaaca ggtaaaaaac aaactgacaa aatctactac tggcacactg
     9661 gctatgtagg tggcatcaaa gatgcaacct tcaaagaaat gattgctcgt cgtcctgaag
     9721 ctgtgattga aatcgcagtg aaaggtatgt taccaaaagg tccacttggt cgtgaaatgt
     9781 tccgtaaatt aaaagtttac gcaggttcag aacacaatca ccaagcacaa caaccacaag
     9841 ttttagatat ctaattcacg gagcataaaa atgacagcag caaatcaaaa ctacggcaca
     9901 ggtcgccgca aaagctcttc agcccgtgta tttatcaaac cgggttcagg taacattact
     9961 atcaaccaac gttcacttga agtttacttc ggtcgtgaaa cagctcgtat gatcgtacgt
    10021 cagccgttag aattggttga attaactgat aaattagacc tttacatcac tgttaaaggt
    10081 ggtggtattt ctggtcaagc gggtgcaatc cgtcacggta tcactcgtgc attaatggaa
    10141 tacgatgaaa cgctacgccc agcacttcgt gcagcaggct tcgttactcg tgacgcacgt
    10201 cgcgttgaac gtaaaaaagt gggtttacac aaagcacgtc gtcgtccaca atactcaaaa
    10261 cgttaatttt cttttcgttt tcaagaaggc agggtttcct gccttttttg tctttatgac
    10321 tgtggtagaa taaccgccct gattaactat ttgatagagg catttatgtt agccattatt
    10381 tcccctgcca aaacccttga ttttaaaacc aatattgacg gttttgcatt ttctcaacca
    10441 aatttgaccg cttgtagcca agaattaatt gatatttgta aacagctttc accagccgaa
    10501 gtaggaagtt tgatgtcaat cagcgataaa ttagccgctt taaatgtcgc tcgctttgcc
    10561 gaatggcagc ttgagcataa ccaacaaaac tctaaagccg ccatttttgc ttttaaaggc
    10621 gatgtttaca cagggttaga tgcagaaacg ctaactaaag agcaggttga atatgctcag
    10681 caacatttac gtatgctttc aggcttgtat ggtttattaa aaccgctcga tttaatgcag
    10741 ccttatcgtt tagaaatggg aactaagttg gcaaatccga agggcagaga tctttatgcg
    10801 ttctggggta atattattac tgaacacttg cagcaagcca ttgatgaaca aggcgatgat
    10861 attttaatta atcttgcctc tgatgaatat tatggtgcag ttaagccaca aaatctaaat
    10921 gcgactatta tcaagccggt gtttttagat gagaaaaacg gcaaatataa agtgatcagc
    10981 ttctatgcca aaaaagcccg tggaatgatg gtccgcttta tgttagacac ccaaccgact
    11041 agcatcgaac agctcaaaca gtttaactat gggggctatt ggcttgatgc agatagttct
    11101 agtgaaaacg agctggtttt taaacgtgag gagcaggcat aatgtcggct accaaaaagg
    11161 gaataacttt ggttgcgtta tcattggcag gttgtgtcag ccaaagccca caatttgcag
    11221 tgcctgatgc tattcgcttt gggcaggata cttttgtaaa agttactcac agccaaattg
    11281 acgaaatgca acatttactt tatttgcccg aaaagagtga gaaaaatctg gaaaattggc
    11341 aaaaaggcat tttatttttc ttggataaaa acagtaaaaa tcaaacctta gagcaacgag
    11401 ctgcattccg ccaagcaagt tttgcgaaaa agccggagct tgaagcaaaa gtggcaatcg
    11461 aacaaaatga attacacagt agtgtgattt acccaccgac agaacgtttt aacaatgtga
    11521 tgttagaagt aacccgtggg cggaatttag cttgtggtta tgggcagatt caatttgcag
    11581 ataagcggga agttagcaag gcaattgcaa aaaaattgcc aaatttgacc gcttacagcc
    11641 aagaaattgc caaactttct cttgaattaa accaactggg ttggcaagtc caatgcagta
    11701 aataatgtag taaatgaacg aaaaatacag caaattagtc aaaagtgcag caaatttagc
    11761 gattattgtt gcgatcttac tgattgtgat taaagccttt gcgtggtgga aaaccggcgc
    11821 aatttcaatc ttggcagcaa tgacggactc ggtagtagat ctgtttgctt cgctgactaa
    11881 tattttggtg ttgcgttttg cgttgcaacc ggcagacgag aatcacgcct tcggacacgg
    11941 caaagcggaa tctttagcgg cacttgctca aagtgccttt atttccggct cggcgctttt
    12001 cctgctgcta caaggcttcc acaaactgac taatccggaa ttaattgctc aatctgatat
    12061 tggggtaatt gtgagtattg cttccattgt gatcaccgca cttttagtct tttatcagcg
    12121 ttatgtcgtt acgctcacga aaagtccggc gattgaggcg gattcattac attaccaaac
    12181 tgatttatta atgaacgcag caattttagt ggcaatgttg ctcaatagtg ccggtgttgt
    12241 ctatgctgat gcggtttttg caattggtat tgccctctac attgccttta atgcagtgaa
    12301 aatgttttgg tgtgcggtac aaactttgct agaccaagcc ttgcccgatg aagaaattga
    12361 acagataaaa cagattgctt cgcaacatcc gaacatttta gggattcacg atattctaac
    12421 ccgccgtgca ggtgcggtac ggtttatcca actacatttg gaattagacg atcacttaac
    12481 gctgctggta gctcacgaaa taactgatag ccttgagcag aaattgctgg cggcatttcc
    12541 aatgtctcac gttatcattc atcaagagcc gactacggtg gtcaaacaag aatttatggc
    12601 ggagaatatt taatgtatct ggcaatcgtg catttcagct accataattt tgagcaagat
    12661 ccggtgacgg caatcagcca agtcttaaat cagtggttat ataacgggca agtcattggc
    12721 agagaaatgc cgattacctt tcaccaaaat gagtttcaag ctcgggtttg tgtgccgaca
    12781 caagaaagcc ttttgccaca atataatagc gatgaagtga atgaagcgtt gcaacaagcg
    12841 gttgaaattg gcgtgaattt tgtaagtttt gagcttgtcg ggcgagatta tcagggcgaa
    12901 gaaagcagtc ataaccgatc gccagcgttt caaatattat ataccaccca tttagatacc
    12961 tgctcgccac tctatgatgg agagcagttt gccccaattc cgctttattc tttacaggat
    13021 cagtctctta gcgaagcgat attggaatgg cagcaaaatt ggcaagcgtg cgatctgcta
    13081 caaatgaacg gcaatgtgct agagtgccac gcacttgctc aaattagcga tgaaacaagc
    13141 gttcttgcaa cgatcggtcg agatctatgt cagcaaattg agcagagaac ccacattccc
    13201 accttttatt acctctaccg tttagggcgt gatgaaggtg aatatcaacg caaatgccct
    13261 tgttgtggcg gagaatggaa attaactgag ccgttgcatg atattttcca ttttagatgt
    13321 gatccttgcc gtttgatctc taatctaagc tgggaattat tataactttt agttatacca
    13381 aagtaataaa tcatcttgaa tgaatgtcgc aagcgtaata ctcttgcggc atttttataa
    13441 taagcggttt tactttggag gttctgtgct tattttcaac ttaattgtct ttgcggcaat
    13501 actattttta ttggctaaaa tttataaatc tacgcaaaaa ctcggtcaaa cggtgtttat
    13561 tgggttattt ttagggcttg caagcggtgc ttttttgcaa actttttacg aaaaagctac
    13621 tgttgatgca actcttgatt ggattaactt aattggtaac ggttatgttc gcttgctaca
    13681 aatgatcgta atgccattgg tgtttgtctc tattttatcg gcaattaccc gcttaaaaga
    13741 ggcaggatct ttaggcaaaa ttagttttag tgtgctatca gtgttattag tgaccaccgc
    13801 gattgccgca gcgattggta ttgctttagt gtatctgttt gatttatctg ccgagggttt
    13861 agtcgccggc tcccgtgagc ttgccgctca agagaaagtg gtagggcgtg tggagcaggt
    13921 gagcaactta tctgtgcctt caatgttgct ttctttcatt ccgaaaaatc cgtttttaga
    13981 attaaccggt gcaaacccaa cgtcgatcat caacacggtt attttctcgg ctctgctagg
    14041 cattgctgca ctgagcttag gcaaagaaga taaagcgttg ggcgaacgta tcgcacaagg
    14101 tgtagagacc ttaaataaac tggttatgcg tttagtacgt tttgtgattc gcctaacccc
    14161 ttatggcgta tttgcgttga tgatcaaaat ggcggcaact tctcaatgga gcgatattgt
    14221 aaatttaggt agctttattg tggcatcata cattgcgatt ggtttgatgt ttattgtgca
    14281 cggtatttta ctctttgtgg ctaaaatcaa cccgttggat tactacaaaa aagtgttgcc
    14341 gacattaagt tttgctttta cgtctcgttc cagtgcggca accattccgt tgaatattga
    14401 gacgcaaacc aataattagg taataacagt gtgattgcca acttttctgc aacctttggg
    14461 gcaaccatcg ggcaaaatgg ctgtgcggga atttatccgg caatgttggc ggtgatggtc
    14521 gctccaacag tgggaatgga tccgtttatc ttaaactata ttttaacctt gattttggtg
    14581 gtggcaattt cctcatttgg cattgccggt gtaggtggtg gtgcaacttt tgcggcgatt
    14641 gtggtgctat caaccttgaa tctgccgatt gagttagtag gcttgctcat ttcagttgag
    14701 ccgattattg atatggctcg caccgcctta aacgtgaatg gtgcaatggt ggcgggcaca
    14761 ttaaccaata aatgggtgaa agccgagtag tcaaaactaa agatataaaa atgggcgatt
    14821 aattcgcccg tttttataag ttaaggtgat tattcaatcc acgcaatcac ttcttcggta
    14881 ggtttgcggg attttttctt aatttctttg ttgcgatagc caaaagttat catacaagac
    14941 acaccatatt cgtttaggtc aaataagcct tcgtccgata aaatttggtt tacggtatcg
    15001 taatgaaaac cttcaatcgg gcaagagtca ataccaatca gagccgcacc ggtcatcata
    15061 ttgcctaatg caatataggt ttgcttggta ctccaatcga ataattcacg ttcactaagc
    15121 aatttcatat cctcttcttg gaacgactta tagcgtacca gcgaggcttc aatctgctca
    15181 taggttaagc ctcgtttggc taacatatca cgcatataga cagaatcata acggacattc
    15241 tttttggtta aaatcaccac taaatggctc atttcttcca ttgggtgttt aattccccac
    15301 gaaaccgggg cgattttctg acgggtttcc gggttttgta gcactaaaaa tttccacggt
    15361 tcagaaccga cagagcttgg tgagagacgc ccgagttcca aaatataatc catatcttct
    15421 ttgttgattt ttttgtttgg atcgtaactg cgacaggccg aacggaagtg aaaagcgtct
    15481 aaaacttgct gtttgctaat attaaacatc gtttttcctt ttataagtga aataagtaga
    15541 ttaatgataa cttgatttag agcagaatgc gaatttcacg cctgttaatt tgcaaaaaat
    15601 taggaaaaaa tgaccgcttg tttaaaattg ctttacctta aaaatcaatc atttaacgaa
    15661 tcggttgatt tactttttct ggctaaagta ttattattca tgaaattttt tatttcattg
    15721 agtttagagg ataggtaatg caacacctaa aagatttaac taataaagca cgtgaagctt
    15781 tagataagat tgatcaaaat ttagaggctt tagaagagtt tagagtcgaa tattttggta
    15841 aaaaagggca ttttacaacg ttaatgcaag aattacgcaa tattgcagcg gaagaaagac
    15901 cggcaatcgg agcaaaaatt aatgaagcaa agcaagttgt gcttgatgtg ttaaatgcca
    15961 aaaaagaaga atgggaacaa gctgtattaa atgcaaaatt agctaatgag agcattgatg
    16021 tttcactgcc ggggcgtaaa actgaattag gtggtttgca ccctgtttct atcactattg
    16081 agcgtgtggt gaaatttttc tctgaattag gttttaccgt agcaaacgga cctgaaattg
    16141 aaaccgatta ctataacttc gatgcgttaa acattccgga tcatcatccg gctcgagcgg
    16201 atcacgatac gttttggttt gatgccaatc gtttattaag aacccaaacc tcgggtgtgc
    16261 aaatccgtac aatggaaaat gtaaaaccgc ctatccgtat tgtggcaccg gggcgtgtat
    16321 atcgtaatga ctacgaccaa acccacacac cgatgttcca ccaaatcgaa ttgttatatg
    16381 tggataaaaa agcaaacttt accgagttaa aaggcttaat tcacgatttt ctcaaagcat
    16441 tctttgaaga agatttacaa gtgcgtttcc gcccgtcatt cttcccattc actgagcctt
    16501 ctgcagaagt agatgtaatg cgtcaaaacg gcaaatggtt agaagtgtta ggttgcggta
    16561 tggtacaccc gaatgtgtta cgcaatgtag ggattgaccc tgaagaatat agcggttttg
    16621 ctgtgggaat gggcgtcgag cgtttaacga tgttacgcta caatgtcacg gatttacgtt
    16681 cattctttga aaatgactta cgtttcttaa aacaatttaa gtaatacaag cggttaaaaa
    16741 tttagaaaat tttacaaata ggatagaaat aatgaaattc aatgaatctt ggttgcgtga
    16801 atgggtaaac ccagcaatta gcacagaaca gttatgcgac caaattacaa tgttagggtt
    16861 agaagttgac gatgtagagc cggtagctgg tgcgttttcg ggtgtagttg tgggtgaagt
    16921 ggtggaatgc gcccagcacc cggatgctga caaattgcgt gtcactaaag tgaatgttgg
    16981 tggcgaacgt ttattagata ttgtttgtgg tgcaccaaac tgccgtcaag gtttaaaagt
    17041 ggcttgcgct gttgaaggtg cagtgctacc gggcgatttc aaaattaaga aaaccaaatt
    17101 gcgtggtcag ccttcagagg ggatgttatg ttcttactca gaacttggga ttaaagaaga
    17161 tcatagcggt attattgaat tacctgctga tgcacctatt ggtcaagatt tccgtgaata
    17221 tttaaattta aatgatgtgg cgattgaaat cagccttacg ccaaaccgtg ccgactgctt
    17281 aagcattgcc ggcattgctc gtgaagtggg ggtgattaac cgtgtcgatg tgaaagctcc
    17341 aactattaca gatgtaaaag ccacgatttc agataaagtt tcagtggaat tacaagcacc
    17401 ggaggcttgc cctcgctatt tagcccgtgt ggtgaaaaat gtgaatgtca aagcgacatc
    17461 gccactttgg ttacaagaga aattacgccg ttgtgggatt cgctcaatcg atccaatcgt
    17521 tgatattact aacttgagcc ttttagaatt agggcagcca atgcacgcct ttgatgcagc
    17581 gaaaattgaa ggtggcattc aagtgcgtat ggcaaaagaa ggcgaagaat tggtattgct
    17641 agatggcaca aacgctaaat tacaatcgaa taccttagtg attgctgata gcaaaggggc
    17701 attggctatg gcaggtattt tcggtggcga agccagcggg gtgagcgaga atacgaaaga
    17761 tgtggtgtta gagtctgcat tttttgcttc attagcgatt accggtcgcg ctcgtcaata
    17821 tgggttacat accgacgcat cacaccgttt tgagcgtggg gttgatccag aattaactcg
    17881 tcaagcaatg gagcgtgcaa cggcattatt actggaaatt tgcggcggtg aagcaagtga
    17941 aattgtggaa gccgtgagcc aagaaaacct accaaaacgc aatactgtca cgcttagacg
    18001 cagtaaatta gattcggtga ttggtcatca tattgacgat gcaacggtaa ccgatatttt
    18061 aacccgctta ggcttgaacg taacatttgc ggataatagt tggacggcaa ttgctccaag
    18121 ttggcgtttt gatattgaaa ttgaagaaga tttaattgaa gaaatcgcgc gaatttatgg
    18181 ctacaacagt atcccaaata attcgccact tgctcattta acgatgaaag gtacgccgga
    18241 aaaattactg gaagcaaacc gtattcgtat ggcattggta gatagtgatt accaagaagt
    18301 gattacttat agctttgttg atccgaaaaa acaatcatta ttgcacccac agcaagaagc
    18361 cttgatttta ccaaacccga tttcaagtga aatgtcggca atgcgtgttt ctcttttaac
    18421 cggtttgttg gacactatcg tgtataacca aagccgtcag caaaaccgtg tgcgtatttt
    18481 tgaagcaggt ctacgtttta ttccggatag caatgcggaa tcaggcgtgc gtcaagaatt
    18541 tgtttttggt gcggcgattg taggcgataa acgtccggta cattgggaaa acaaatcaga
    18601 agcagccgat ttctttgatt taaaaggcga tattgagcgt attctttctt taaccgctat
    18661 ccgccacgat ctgcaatttg tcgcaaaaca gttcccagca ttacacccgg ggcaatcggc
    18721 tgcgattatg cttgatggca aagaaattgg ttttatcgga actgttcacc cgtcaattgt
    18781 gcaaaagttt ggcattaaag gcaaaccgat tgtatgtgaa attctagcat cagctattgc
    18841 agagcgtccg gttacacaag cgggcgaaat ttctaaattt cctgcaaata accgtgatat
    18901 tgcggtagtg gttgatgaat ctgttcctgc aggtgaggtg ttagaggctt gtcgtaaagc
    18961 tggtggtaat aaattagtgg cagtaaattt atttgacgtt taccgtggtt cgaatttaga
    19021 aagtggtaag aagagtttag ccattagctt aacggttcaa gataatgaaa aaacattaga
    19081 agaaaatgaa atcaatgcgg taattcaagc ggtattagct gaattagcaa cacgtttcaa
    19141 tgcggtactt cgtgattaat cagtaaaata tggctgattt attttatcag ccataacaat
    19201 aaactaagga taaatgatgg cgcttaccaa gattgaaatt gcagaaaacc tgattgaaaa
    19261 atttgggtta gaaaagcgtg tagcgaagca atttgttgaa ttgttctttg aagagatccg
    19321 atcctccctc gaaaatggtg aagaagtgaa attatctggt tttggtaatt tttcactgag
    19381 agaaaaaaaa gctcgtccag gaagaaatcc taaaacgggg gaaaatgtgg ctgtttcagc
    19441 ccgtcgagta gtagttttta aagctgggca aaaattacga gaacgtgttg agcaagctag
    19501 ctcccaatca taggggcagt tatattagtt agatttaatg tggagtaata aatgaaatta
    19561 accaaacggt ggctttcctt aggttgtttg ttagctacta cagcgatgtt agtggcttgt
    19621 tcaagttcag atacaacctc taagtctcaa gtaaaaagta aagtttatcg ttcaatatac
    19681 caatcagacc tttctgatcc tattatggca attagtcgat taagtgaaca tcaacacgaa
    19741 tggaaaggca ctcgctatcg tttaggtggc aatagcaagg ctggcatcga ctgttctggt
    19801 tttatgcaag tcacttttaa agatcttttt gggatagatt tgccgagaac caccacagag
    19861 caggctaagg ctggtgagcg tatatccaaa gacgaattgc gtacaggtga tttagttttt
    19921 ttcaagacag ggcgtggtcc aaatgggaaa cacgttggtg tttatgtaaa aaatgggcaa
    19981 tttcttcacg cctcaacaaa gggaggggtg atttattctg atttggattc gccatattgg
    20041 tctaaggctt tctggcaggc tcgtcgttta taatttaggg taacatacat gctgaaaaaa
    20101 atcggacaag cggtcatatt tggacttttt tgtgcaattt taattttgtt tgtagctcca
    20161 attattcaga gtaaagggag ttcgttttca ttatcagcat tcactcaaaa agagattgta
    20221 agctacaatg cagcggttaa aatagcctcc cccgcagtcg ttaacgtata tagccgaaat
    20281 tttagttctg aaaatgaaac atttgaagtt aagaatttag gttcgggtgt gattatgagt
    20341 agcgatggct atattttaac taacaggcat gtgattgaag atgcggatca aatcgtgatt
    20401 gctttgcaaa acggtgctat ttcgactaat gtaaaattag tcggcgatga ttctttaact
    20461 gatttagctg ttttaaaaat tgagggtgag aatttaccta ctattccaca aaatccggag
    20521 cgcaaactac aaattggcga tgtggtgttg gcaattggta atcctttgaa tttaggacag
    20581 agcattacgc aagggatagt cagtgctatt gggcgaaaaa ccttaacaga aagtggtaga
    20641 caaaatttta ttcagacaga tgtttccatt aatcaaggga actcaggtgg tgctttaatt
    20701 aatactgctg gtgagctaat aggtattaac actttgcgtt ttgggaaaaa tacgaatgaa
    20761 ttgtcagagg gcctaaattt tgcgattccc attaatctgg cgaatcaaat tatgagtaag
    20821 atcattaaag atggccgagt tattcgtggc tattttgggg taagtacaga tttgcttgac
    20881 accgcaaaaa tgcaaggtta tgaaaaaggg gtgatcattg ttaatgtaga taaaaatggg
    20941 ccagccgcaa aagcggggat tcaagcaggt gacttagtgc ttaaagtcgg agatattgag
    21001 gcattctctc cttctcaaat gatggaagag cttgctgaaa tgaaacctta tactacagta
    21061 aaagtattgg ttcaacgtga tgaacaatta cttaactttg atgtaactat tactgagtta
    21121 cagactcaat agggtagaaa atgaaaaaaa tagtgattac agttgatggg ccgagtggtg
    21181 caggtaaagg aacgttatgt catgcattgg ctgaaaaatt agggtttgat tttttagatt
    21241 ctggagcctt ataccggatt actgcattaa gcgcaattaa aaaagaggtt ccccttgatg
    21301 atgaggaaaa actagcggaa gttgggcgta atcttgatat tcaatttttg ccagaaaatg
    21361 gcgagattca ggtgattttg gaaggagaaa atgtaggcga cttaatccga acggcagaag
    21421 caggtcaaaa tgcctctaaa gtcgctgctt tccctttggt acgtgaagca cttttagaac
    21481 gccaaagagc ctttagtagc gaaaaaggtc tgattgcaga tggacgagat atggggacag
    21541 ttgtgtttcc tgatgcacaa attaagctgt ttttagatgc aagtgccgaa gaacgcacaa
    21601 aaagacgtgt aaaacagttg caagaaaagg gatttaatgc taactttgac gagattttag
    21661 ccgagataaa agaacgtgat tttcgcgata gaaatcgagc agtcgcgcca cttgttccgg
    21721 ctaaagatgc gttgctgtta gattcaacgc atttatcaat tgaagatgtg attaaccaag
    21781 cattaaatca tatcgctcaa tacattaaat tttagtttgt tgcttcacat aggatttgtg
    21841 tagcttttat tatcaacccc gtttaacagg acgttagatg gatgtttatt tttaagaaga
    21901 tttattatgt cagaattatc gtttgctcaa ctacttgaag aatcatttgc agctacacca
    21961 cgtttaggtg atgttgttaa aggtactgtt gttgctatcc aaaaaggctt agttattgtt
    22021 gatacaggtt tcaaatctga atctgcaatc gatgcctcag aatttactaa cgcacaaggt
    22081 gaattagaag ttcaagtggg tgatgaagta gatgttgttc ttaaagctgt agaagacggt
    22141 tttggtgcaa cagtagtttc tcgtggtgat gcaaaacgta acgaagcttg gattgcttta
    22201 gagaaagcat ttgaagaaca agctacagtt atcggtttcg taaatggtaa agtaaaaggc
    22261 ggtttcactg ttgaattaaa cggtgttcgt gcattcttac ctggttcatt agttgatact
    22321 cgtccggttc gtgattcttt aaacctagaa ggtaaagagt acgaattcaa agtaatcaaa
    22381 ttagatcaaa aacgtaacaa cgtagtagta tctcgtcgtg ctgttatcga aacagaaagc
    22441 aaccaagatc gtgatgcggt attagcaaac ttagttgaag gttctgaagt taaaggtaca
    22501 gttaaaaact taactgacta cggtgcattc gtagatttag gtggcgttga cggtttatta
    22561 catatcacag atatggcttg gaaacgtgtt aaacacccaa gtgaagtggt taatgtaggc
    22621 gatgaagtta ctgttaaagt attaaaattt gacaaagaca aaactcgtgt atcattaggc
    22681 ttaaaacaat taggtcaaga tccttgggct gtaattgctg aaaaccaccc ggtaaacagc
    22741 aaattaactg gtaaagtaac taacttaact gactacggtt gcttcgttga gattttagac
    22801 ggtgttgaag gtttagttca cgtttcagaa atggattgga caaacaaaaa cattcaccca
    22861 tctaaagtgg ttaacgtagg cgatgtagtt gaagtaatgg tacttgaagt tgatgaagaa
    22921 cgtcgtcgta tctcattagg tttaaaacaa tgtaaagcaa acccttggga acaatttgcg
    22981 gcaactcaca gcaaaaatga taaagtatca ggtaaaatca aatcaattac tgacttcggt
    23041 attttcatcg gtttagaagg tggtattgac ggtttagttc acttatctga catttcttgg
    23101 aatgtttcag gtgaagaagc agttcgtaac tacaaaaaag gtgatgaagt agaagctgtt
    23161 gttctacaag ttgatgcagt gaaagagcgt atctctttag gtatcaaaca attagaatct
    23221 gatcctttca caaactttgt agatggtact aaaaaaggtg caatcttaac aggtaaagtt
    23281 gtagaagttg atgctaaagg cgttaaagtt gaattggaag gtggcgttga agcatttatt
    23341 cgtgcaaacg aagcaactgc tgagcgtgta gaagacatca cttctgttat ttctgttggc
    23401 gattcaatcg aagcgaaata caccggtgtt gatcgtaaat ctcgcgtagt taacttatct
    23461 gttcgtgcaa aaaacgaagc tgaagaatca gcagcaattg cacaagtgaa caaagaggaa
    23521 gttgttgtac ctaatgcatt tgctgaagct ttcaaagcag ctaaaggcga ataattagta
    23581 tagactaatc taaaatgggt atgttttgaa catacccatt taaacttaga aatgccattt
    23641 tgaatactta ttttcataat aaatgattat tcaaaataga attaattttt ggtagaaata
    23701 atatgactaa atctgaatta attgagagtt tagcatctaa aaatccaagt ttaccaatta
    23761 aaatggtgga gcattgtgta aaagagcttt tagagcagtt aacggctact ttagaagaag
    23821 gtgaaaggat agaagtaaga ggatttggta gtttttctct acattatcgg cagccgcgtt
    23881 taggtcgcaa tcccaaaaca ggagaaagtg ttcttttagg cgcgaaatat gtaccacatt
    23941 ttaaagctgg taaagatcta aaagagagag ttgatctgct ttaagtctta ttaattcaat
    24001 aatcgtagta aggagattat atgattaagt atattttagg tattttaatt gtgattgcag
    24061 ttattattgt cgctgttacg attggtgcca ataatgatca acttatcacc tttaactata
    24121 tttttgctca gagtgaatta cgcttatcaa cgttagttgc gattttattt ggcttaggat
    24181 tgattcttgg ttggttggta acgggtattt tttacttaaa aattaagttt caaaacgttg
    24241 ctttaaatcg tcgtgtaaag cgccaagctc agcaaataac tgaattaacg actccaaagg
    24301 ctgaataatc aatgcttgaa ttattgttcc ttttattgcc tattgcagca ctatatggct
    24361 ggtatatggg acaacgtagt gcaaagaagg atcaagaaac cgctagtaat aagttctctc
    24421 gtgagtatgt tacggggtta aattttcttt tatctaatca gcaggaaaag gcggtggacc
    24481 ttttcctctc tatgttacaa aaacaagaat ccgaaaatca tattgcatct gaatcccaat
    24541 ttgaagcgga gttaacccta ggtaatcttt tccgttcaag aggtgaggta gatcgtgcct
    24601 tacgtattca ccaaacatta gtgaatagcc ctcattattc atttgatcaa aaattattag
    24661 caaaacagca attagctaaa gactttatgg ttgcaggttt ctatgaccgt gcagaaaatt
    24721 attacatttc attagttgat gaacctgagt ttgctaaata ttctttatct cagcttgctg
    24781 ttatttacca aaaaaccaga gaatggaaaa aagcgattaa tgttacagaa aaacggcttc
    24841 gaatagagcc ggatgcagat aaaataccgc tttcacattt ttattgtgaa tatgcgcaag
    24901 gggttaaatt tgatgataaa gcggcattta ttgaatcgtt aaataaagcg ttagaatatt
    24961 atcctcaatc cacacgcgct tctataatat taggtgattt ctatttagaa gagggagaat
    25021 atctaagagc attaagctat ttcgaacaga ttcttgtgca agaccctgat tatattagcg
    25081 aagtgcttgg acgaattaag caaagctata tggctcttga ggatttaaat ggctatgagt
    25141 tatttctcat tcgagcaaac cgagtaaaac ataatagtag tgtagatatt gctttaactg
    25201 aatttattga agaaaaagat ggtatcaatg ctgcacattc taaactatac cagcagttga
    25261 gtacttatcc gaatttaatt acttttcacc gctttattcg ctatcaagca gattttgcgg
    25321 aggaaggaaa tggtaaggaa agcttagttt tattacataa tatggtggga aatcagataa
    25381 aacgcagctt ccaatatcgc tgtttaaatt gtggttatca aagttatcgc ttaatgtggc
    25441 aatgtccttc ttgtaatcaa tgggaaaaaa ttaagccggt acaaagtatc gaaggtatta
    25501 ttcaataaag aaagaatctg aggaattgaa aatgaacaat aaagtaattg ttgcgttaga
    25561 ctatgaaact gaacgtgaag cattagaatt tgtggatttg gtcgatccgt cgctttgtcg
    25621 tttaaaagta ggcaaagaaa tgtttacaac cttaggcaca aattttgtca aacaattaca
    25681 cgaacgtaaa tttgatgtat tccttgattt aaaatatcat gatattccaa atacggttgc
    25741 tcgagcagta cgttctgctg ctgatcttgg tgtgtggatg gttgacttgc atgcttctgg
    25801 tggtttagcc atgatggaag aggcaaagaa aattttggag ccttatggca aagaggcacc
    25861 attattaatt gcagtgacgg ttctaaccag tatggaagat ttggatttgc ttcaaatagg
    25921 tattaatgct tcgccgatgg aacaagttat tcgtttagca catttagcac aacgggctgg
    25981 tttagatggc gtggtatgtt ctccacaaga agttgaggta ttacgtaccc attgtggtca
    26041 agattttaaa ttagtgaccc caggaatccg gccaaaagga acagattttg gggatcaacg
    26101 ccgagtgatg acacccaaac aagcagtaga aacaggctca gattttttgg ttattggtcg
    26161 tcctattacc caagctcaag atccgcttgc ggtattaaaa tctattaacc aatctatcag
    26221 ttaattcatt gcatttttaa tgaaggcgga aaaatttccg ccttttttaa tcaaacaagc
    26281 ggttattttt gaggaaaaat ttgtaaatga cattagtcta ttcaacggaa acaggcagaa
    26341 taaagtccga agtaataaaa gaagagcgcc ctaaagggga tggtattgtg cgtattcaac
    26401 gtcaaacaag cggtagaaag gggaaaggtg tttgccttat caccggtctt gatttagagg
    26461 ataaagcctt aaattctctt gcttcagagc ttaaacgtaa attgggttgt ggtggctcat
    26521 ttaaagatgg agtaattgaa atccagggcg ataatcgagg attcattaag caaatcttag
    26581 aacagaaagg tttcaaagtg aaactggcag gtggttgagt tttaaacaat tatcagctgg
    26641 cagtttttaa ataacttcat tttatttgaa acttcattcc tacttttctg cctaaaactc
    26701 acgattaagg agaaaaacta atgtggaaga aaacgttgat tgctgtattt ttagcaagcc
    26761 aatatgcttt tgcggtagga accgggaaag aaccatcaaa aagttatact gaaacagaat
    26821 taaaacaatt aaaaaatgat tggttagcga tttatcaaaa agagctacag tatgcagaaa
    26881 atttgcgttt aaaacagaga gaaaattttc agcaaattaa ttatttggca gatgcagcga
    26941 ttagtcagaa gaatttatca ctttcaacgg agcagataat tcagcgttta ttactaacct
    27001 tagcgggtta tccgttggag atgaaagctg aatggaagct gctaaatgct aaaataacgt
    27061 taaaacaagc tacagaaagt gaagttaaag catttatttc aaaatatcct gattctttgt
    27121 atcaaaggca gttacagaac ttgatgtttg agctgctcta tcgtgaacag aaatttccgg
    27181 agttaatgga atatgctcaa aatgtaaaac cgagtagtct tgccgaccaa tgccgtttat
    27241 tttctgctcg ttatgaactg gcagcaagta aagctcaaat caatccggaa gtttctcaag
    27301 taactgagca aaaagcagaa aatgcagaac tgcttgcttt ggtgcaagag tttgatcgct
    27361 tttggcttgc tacgccgaaa ttaacttctg attgtgcgaa tttagaaagt tattggcggg
    27421 atcaggggtt taaaaccgat gaaaaagtgc gtttgaaagc ggtcgaatta attaaacaaa
    27481 atgcaaatgc ggaattggcg aatttagcgg taaatgtaaa caatgagaat ttgaaaatct
    27541 ggctatcttc cgttgaaaaa gtagcgaata atccggcaga tttgcaaaat tttatcgaaa
    27601 atcaaccgct tgattctccg ttttatgctg agaataaagc gattattcag caactctttg
    27661 ctaaatatgt gcgaacttta tcggaaaata tggagaaccc aagtttccag cagtaccaag
    27721 cgtgggcgga gaagtatcaa ttttccaatg aggaaatgaa tagctggaaa aataccttta
    27781 tcactcggct ttttgataat ccagaaccaa cattccagct ttggcgtgat gagcaaatta
    27841 aactgctcaa tacagacaat ttaaccgaac gccgtttacg aatggcaatt tggcagcaaa
    27901 cagcgttaaa tgagtggttg gatttactct caactgaggc gaaaggtaaa acagaatggc
    27961 gttattgggc agcgaaagag gagaaaaatg aagcgaagcg taaagctgat tttgaaaact
    28021 tagctaaaga gcgaggcttt tatgctatgt tggctgctca acaattaggc attatttacc
    28081 aaatcccaat gctcgaggta cctgaattga cgcagactca gcttgaattg tatgaaaacc
    28141 aattagcgcg tattcaagag ttacgagagc tagggcagtt tgagcaggca agaagagtat
    28201 ggattgactg gctaaaaaac attaaagcgg aagcgggtga aaatacacca acgaaaatcc
    28261 aattagccct gataaaatac gctaaggata aaaattggta tgatttggcg gtggaaggta
    28321 cgattcaaat aaaagccttt gattatcttg atttgcgatt accaaatgcc tattcggatt
    28381 ggttcgattt acatctgcaa gataaaaaaa tcagcaaaac gtttgctcag gcgattgctc
    28441 gtcaagaaag tgcgtggaat gctcaggctc aatcgcacgc taatgcgaga gggctaatgc
    28501 aactgttacc gacaacagca caaaaaaccg ctaaagataa cggtttaacc ttcaaagatg
    28561 gcaatgatct ctttcaaccg tttaacaata ttatgttagg cacaacccat ttaatggagt
    28621 taaacgaaaa atatccgaat aatcggattc tgattgcctc tgcttataat gcaggagcag
    28681 ggcgtgttga gcagtggctg aaacgtagta acgggcagtt agcaatggat gaatttattg
    28741 ccagtatccc gttttatgaa accagaggct atgtacaaaa tgtgctggcg tatgactact
    28801 attatcaaat gcgctatagc gatataaatg ataaaaatgg attaaaaatg ttctatcaag
    28861 aggaattaac aagaaaatat taaaaaatgc ttgtgttttt gtgtagaacc gctataatgc
    28921 accctatcag
//
LOCUS       DANKBV010000015        27630 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.015, whole genome shotgun sequence.
ACCESSION   DANKBV010000015 DANKBV010000000
VERSION     DANKBV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27630
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.015"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..615)
                     /locus_tag="PWY43_000528"
     CDS             complement(<1..615)
                     /locus_tag="PWY43_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2135436.1"
                     /translation="MALLDIRHLSIEIDTPKGRVKMVDNINLTVDEGEICGLVGESGS
                     GKSLIAKVICGMIKDEWIVSADRFRFDDIELLKLSPAERRKLVGDHISMVFQDPLTSL
                     DPSQQIGKQLMQTIQFRGKWWQRFGWKKKKAIELLHRVGIRDHQDIMKSYPSDITEGE
                     AQKVMIAMAVANQPRLLVADEPTNIMESTTQLQVYRLLSSMNQNL"
     gene            complement(686..1579)
                     /locus_tag="PWY43_000529"
     CDS             complement(686..1579)
                     /locus_tag="PWY43_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2135437.1"
                     /translation="MAVIKEPEQFRETNAFKAFWFHFRQDKLALGSFYLFLLLLALIF
                     VGSLLAPYNYNEQFVGLELMPPSWDDKGQISHFLGTDDLGRDILSRIMYGFYYTLGSA
                     LMITLLIAIIGGMIGIYAGLKKGRSFWLISHLFDTFLFMPILLIAIIIATLMEASLVN
                     AMLAIFLAMLPHFIHKIYQATEKQLKKEYIITLKLDGASHWYLIREVIIPNLTGVAIK
                     ALSHIFVIAILDISALSFIMLGAKSPTPEWGAMIRESMELIYIAPWTVILPGLAIIFS
                     IFTVTILGTRLSVIFEKYRKE"
     gene            complement(1566..2531)
                     /locus_tag="PWY43_000530"
     CDS             complement(1566..2531)
                     /locus_tag="PWY43_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2135438.1"
                     /translation="MLIVFFRKIFLTLITLIFLSLISFNILLRDPLNHLAELGWFEAY
                     CYYVKSLLNGDFGISFSNGEPLTAQILQVFLATITLCLSALIVSLIIGLPLGFFAATQ
                     QKNMVGRLLNTLGSLSLAVPVFWLALVLMYYASLNQWEVSAIGEIHSLYPKQMVTGFL
                     LLDIWLSDVPYKLKMMQSALHHLALPTLVLAVPATLEVMHITQARAGYVMKQNYIKIA
                     KTRGWTPFRVWRTHIICNTLPALLPMIARTFILIFAFGMLIENIFSWGGIGRWLINAL
                     AIQDYNAISAGVVAIGVFVLLVDMVTGFVRVMLDPSDKKDWYGSY"
     gene            complement(2534..4285)
                     /locus_tag="PWY43_000531"
     CDS             complement(2534..4285)
                     /locus_tag="PWY43_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2135439.1"
                     /translation="MNKLAVIFSVCFANLGLNSTAYAAPSIPQELLENSLVYCTTSSG
                     FSFNPQKSDVSSNMNVVTEQIYDKLVEFNPKKNRLEPALAESYSISDDGLVITLRLRK
                     NVAFHTTPWFTPSRKLNAEDVVFSLKRMIGKESDLPEVKSEPESVLHHQRQSYIYQKT
                     AGQIYFPYFESVDLANKIASIHSPSNDIVKIELVRPDSALLAHLASQYAVIMSKEYAL
                     QLNADDNLVQLDLLPVGTGVYQLESYENNDYVRLKPNMNYWGKKAYIQNMVVDFSTEA
                     TGRMAKFLNNECDVVAFPEPSQLSVLNNRQLISSKGANLVYLAFNMQRGIGQDLALRQ
                     RIARGINRKRIAEKLFYGTGEVADNLLPTALWSKKNNGSYPYQPHQNSGEQAVEMHDS
                     IINRSLDSKTETEEVNQKQRLKLWVIDEKRVYNQHPLKMAEMIRADLATQNLEVEIRA
                     VSRAYLVQQLANKSADYDLILGGWLANNLDPNSFLEALLSCKAAETVTNLSNWCNEDF
                     DFWLQTARLSDDPASANLLYEFAQKLLEEQLPILPLMNANRVLVIKDGVKQADISPFG
                     QVKLSELRLGLGHKKGN"
     gene            complement(4278..5237)
                     /locus_tag="PWY43_000532"
     CDS             complement(4278..5237)
                     /locus_tag="PWY43_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2135440.1"
                     /translation="MVVNPESFEQIIGQSVKIKEVVEQAKKFANLDAPLLIQGETGTG
                     KDLFAKSCHHFGSRRMQKFIAVNCAGLPAEEAESEMFGHRGNGVESVGFFEYAHGGTV
                     LLDSISELSLEMQAKLLRFLNDGSFRRVGEDQEIQVNVRVICTSLQPLSLLVEKGKVR
                     EDLFHRLNVLTLNLPPLRDRKEDIPLLADYFIAQISNQLGVSKLEYDNVFLQALQNYH
                     WRGNLRELYNALYRACTLSHSYRLAVKDLHLPRDMEAMIEVREEATLEEMMSQYEAQL
                     LHQFYAEYPSTRKLAQRLGLSHTAVANKLRQYGLTKNGNSKDE"
     gene            5546..6127
                     /gene="lpcA"
                     /locus_tag="PWY43_000533"
     CDS             5546..6127
                     /gene="lpcA"
                     /locus_tag="PWY43_000533"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDL2135441.1"
                     /translation="MYLEQIKAELQEAADVLDKFMSDEKNIKLIQDAAMLIANSFKQG
                     GKVISCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHLSCVSNDFGYEYVFS
                     RYLEAVGQKGDVLFGLSTSGNSKNVLNAIKVAKEKGMKVIAMTGKDGGQMAGLADVEI
                     RVPHFRYADRIQEIHIKVIHILMMLIEFEMEKA"
     gene            complement(6168..6620)
                     /locus_tag="PWY43_000534"
     CDS             complement(6168..6620)
                     /locus_tag="PWY43_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDL2135442.1"
                     /translation="MQFEQHFEANFWQNKSLLEMNDAEWEALCDGCGKCCYRKYIQGR
                     GRRERLYYTSVACNLLDVNTGRCMNYSNRFKIESDCTKLTKKNLPDFGWLPKTCAYRL
                     LYEGKPLFDWHPLISKDPDSVKNAGMLIANGIHEKDVIDWFEFVIDEV"
     gene            complement(6624..6944)
                     /locus_tag="PWY43_000535"
     CDS             complement(6624..6944)
                     /locus_tag="PWY43_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HDL2135443.1"
                     /translation="MIITTTPTIEGKQIVEYKGLVFGEVVSGANFIRDFLAGITDIIG
                     GRSGAYESKLNGARKEALAELAKEAQKVGANAVVGVSMEYQSMGGDKGMFIVVAIGTA
                     VVIR"
     gene            complement(6941..7540)
                     /gene="rdgB"
                     /locus_tag="PWY43_000536"
     CDS             complement(6941..7540)
                     /gene="rdgB"
                     /locus_tag="PWY43_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDL2135444.1"
                     /translation="MEKTKIVLATSNADKVKEMADVLSQFGFEVVAQSEFGIESPEET
                     GLTFVENALIKARFAAKMTSLSAIADDSGLSVMALGGEPGLYSARYAGEQATDADNRQ
                     KLLAKMQGQENRLAKFVSCIVFLKHETDPTPYIASGECFGEILTEERGENGFGYDSLF
                     FYPPKACTFAELETKEKKAISHRAIALEQLKQQLAGENK"
     gene            complement(7583..8101)
                     /locus_tag="PWY43_000537"
     CDS             complement(7583..8101)
                     /locus_tag="PWY43_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2135445.1"
                     /translation="MQFRKAVEADFETILAIYNQAIPTHQITADLELATTQNRRKWFD
                     FHLETAQYPIWVVEDEQGIAGWFSFSPFYERPAFLHTSEISIYLDRYSKGKGYGSKII
                     EFMQAEMLNHNIHTLMAYVFELNTVSQNLMRKHGFEQWGRFPNIANMGKDEKGEDKWR
                     TLLMMSYQKGIE"
     gene            complement(8152..9843)
                     /gene="narQ"
                     /locus_tag="PWY43_000538"
     CDS             complement(8152..9843)
                     /gene="narQ"
                     /locus_tag="PWY43_000538"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDL2135446.1"
                     /translation="MIKAKYSIARRIGLYFLIMIGFASIISGISLGIMWSNKSDASLI
                     NVSGSLRMQSYRLLSEMDSKKALLPERLLEYHRTLHATELSSLKQSVLLSDSVIESYQ
                     QLLKDWYEMQSYVAQRDKVNYASHIEDYVQRVDDFVFRLQEFAELKLKIATGVIAIAM
                     LLIIALAYIGVWYTRKKIIGPLRQLVKASWQIQNKDFDHVKLAVNEPNELGLLSSTFT
                     EMSNELLKLYTSLEDKAQDKTRRLLAVNRSLLVLYQCSQLLTAKPINKEVLNQVLKTV
                     FDNEHLLGIDLQVYGAEYWNVSIDNKPSLDWVSAEIAIENEKLGILSWKPSLVCPDGR
                     LIQNVSEMIGRSLYVVQMQKQQQQLVLMEERAIIARELHDSLAQSLTFFKIQVSLLKL
                     AKTDEKRNEILTVFEKALNDAYSQLRELLTTFRLTIQEANLQQALEKILESLRPKTSA
                     QIRLSCKLPSHMFNAQQQVHVLQIVREAVINAIKHSQATEINVIAETNDDGEHCLTIQ
                     DNGVGLAAHTQPEGHYGLTIMQERAAELKAEFSIENVESGGVKVQVLLPSMISKR"
     gene            complement(9846..10664)
                     /locus_tag="PWY43_000539"
     CDS             complement(9846..10664)
                     /locus_tag="PWY43_000539"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HDL2135447.1"
                     /translation="MNYQAIAFDLDGTLLSSNATILESSKEAIKKVREKGIKVYFVTG
                     RHHTAVRPYYVELGLDTPVVCCNGTYVYDFKNEKVLSGNPLSAEIASKLVNDAQAQGI
                     HTAVYFEDAMTYESLNIHFTKFQKWVESCAPEVRPNVYQVENFQQEIDKGVTVWKVLI
                     SDPDLAKMQKFVEKLPPDQVSPEWSWVDRVDITSVGNSKGARLAEVLKTEGIDLQKVI
                     AFGDNFNDVSMLETVGLGIAMGNSEPDIQQKAKKTIGSNNEDSIANELKAQFNL"
     gene            complement(10752..11558)
                     /locus_tag="PWY43_000540"
     CDS             complement(10752..11558)
                     /locus_tag="PWY43_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005726415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL2135448.1"
                     /translation="MALAVAACGNLSKVSDEGTTENPVFPKISESEFNHDGSQFGSWP
                     NWENVRQIEKGMNKDQLYNLIGRLHFEEGLYGVREWDYAFNYRENGVHKICQFKILFD
                     KNMNAQSFFWYPNGCNGNSSFTLTGDFLFDFDKATLTTKGKEVVDNVAQQLKASKAQQ
                     VKVAGYTDRLGSAAYNLDLSQRRSNTVKARLVQQGVTAQIEAVGYGKANQVKACDGET
                     GQELKDCLRPNRRVEISANGGVMKQNEGGNVAGPNGPAPLYQTPAYDGSK"
     gene            complement(11727..13448)
                     /gene="recJ"
                     /locus_tag="PWY43_000541"
     CDS             complement(11727..13448)
                     /gene="recJ"
                     /locus_tag="PWY43_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL2135449.1"
                     /translation="MEKIIKRRPLLTTEIVSTNPLLNRIYQSRGIKNTQELEHNLKNL
                     HRPQQLANIEQAVDLLLQAFREKSRIIIVGDFDADGATSTSLALIALRQLGFDKVDYL
                     IPDRFSQGYGLSVAVAEMVLAKGADLVLTVDNGISSTEGIALLKSHQIQVLVTDHHLP
                     PESLPNADALVNPNLAFCEFPSKSLAGVGVIFYVMMALRSRMREQHLFENKPEPNFAE
                     LLDLVALGTVADVVPLDQNNRILVHQGLQRIRSGHCRLGIKALAEVGKRDLSTLQAAD
                     LGFAIAPRLNAAGRLENMSLGVELLICENMELARRLALDLDSLNQTRKEFEQEMKAEA
                     LSICANLPSLAQSEQAHGIVLYQADWHQGVIGILASRIKDQFNRPVIAFAQESEESEY
                     LKGSARSINGVHMRDLLEHIDMRHPDLIEKFGGHAMAAGLTIHQSKLDLFKQIFDQSI
                     NAIIEPEQLQGIIYTDGELNAQEFTLANAEMIRQGGPWGQHFPEPSFEGEFSILQQKL
                     LAGKHLKLMLQSEKGQLFDAIWFNVDVRAFPDLSIKKARLVYRLDINEFRGEKSLQLK
                     VEYLKYI"
     gene            complement(13533..14216)
                     /gene="dsbC"
                     /locus_tag="PWY43_000542"
     CDS             complement(13533..14216)
                     /gene="dsbC"
                     /locus_tag="PWY43_000542"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDL2135450.1"
                     /translation="MKKNLITLGLMCLSTITFASDAKLKSQLEKIGATDVKISDSPLT
                     GFKMATSNEGVVYISQDGRYVIQGKVFELKNGKAIDITNQGLLAELNSYTNEMIVYPA
                     KNEKHVITVFMDITCHYCHLLHTKIKEYNDLGITIRYLAFPRGGMNTQTAKQMEAIWT
                     SADKVKALNDAEDGKLPTQIKTPDIVKKHYQLGIKFGVTGTPNMVTSEGELIGGYVEP
                     KELAKMLSE"
     gene            complement(14226..15755)
                     /gene="mepM"
                     /locus_tag="PWY43_000543"
     CDS             complement(14226..15755)
                     /gene="mepM"
                     /locus_tag="PWY43_000543"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDL2135451.1"
                     /translation="MQHVILARDRKRHKKHFKIAVFLLAVFSLFVGIVLALKDQSSIN
                     NTSSQEDIIAIDKQEKYQSINTNLAENNTLHQEQHSSFINYVTDLLTQSALVIEHDDE
                     IVDDQTATSYEDELVGQDDEVEEGIETVENQVPGEKLPEEAEKALEDLLDVADQALRI
                     QNQFSYTVARGDKLKDVLEQSGLTASVAKNLIKQHSALAQLEPGQQFYWVLDNDGELE
                     YMNWLVSEKEERVYERKENGKFSFQRFEKKGEWKLDVVRGTIQNSFAVSLKAVGLSDR
                     QINQLAVGLQSQIATNKLKKGDRFAILIKREYIDGKVTDLGNVEGIHVISSKKSYYAI
                     QADNGRYYSRYGETLSGGFARHPLLFAARVTSSFNPKRRHPITGRISPHKGVDYGVPI
                     GTPIIAPSDGVVEHVAYQARGAGRYIRIKHGHITTVYMHLSKTLVKPGQSVKKGERIA
                     LSGNTGGSTGPHLHYEFHINGRPVNPITVKLPGSNSGMGNKERQAFLNKAKNVESRLK
                     M"
     gene            15980..16726
                     /gene="znuC"
                     /locus_tag="PWY43_000544"
     CDS             15980..16726
                     /gene="znuC"
                     /locus_tag="PWY43_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDL2135452.1"
                     /translation="MVSLENIEVSFNGQKALQNINLSIYPNSITTIVGPNGGGKSTLL
                     KILLKLLSPNKGKVIHQKGLKIGYVPQKLHLEPSMPITVYRFLALKPHTTKEMINQAL
                     DLFSITHLTENSMQKLSGGELQRVLLARAILDRPQLLVLDEPMQGVDIIGQTELYQLL
                     NKTRDLLNCAILMVSHDLNIVMANTDEVLCINKHICCAGSPEVITHDPSFIHFFGDQF
                     ARNVAFYSHKHNHHHNLHGDICLGECSCEH"
     gene            16891..19704
                     /gene="sucA"
                     /locus_tag="PWY43_000545"
     CDS             16891..19704
                     /gene="sucA"
                     /locus_tag="PWY43_000545"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDL2135453.1"
                     /translation="MQYTHFDDWLASSALGGANQSYIEEIYEQYLENPANVDESWRNI
                     FDSFPKTPQFEQAHTPIRDYFRKLAREQQPQSVTIIDPEAGAKQVRLLQWINAHRFRG
                     YLEAKLDPINYYRWKTSSVPELDYKYHGFTDEDLNETFTVGRYVYDKDTMKLSELDKA
                     LKETYLGPIGLEFMHVQDIEQRNWLQAKMESVLNKPLFTKAEKLNLLSELTAADGLER
                     YLGAKFPGAKRFSLEGSDAFIPMMKEIIRHASRQGMTDVVLGMAHRGRLNMLVNVFGK
                     RPAELFDEFAGKHADESRTGDVKYHQGFSSNFAVDDKNVHLTLAFNPSHLEIVSPVVI
                     GSVRARQERMRDTEHEKVLAVTVHGDSAVAGQGVVQETLNMANARGYKVGGTIRIVIN
                     NQIGFTTSNPNDTRSTEFCTDIAKMIQAPIIHVNGDDPEAVAFAARMAVEYRTKFKRD
                     IFIDLISYRRHGHNEADEPLATQPMMYSIIKKHPTPRKVYADRLITEGVLTNEQEIEM
                     LNLYRDALDNGECVVPEWREMDMAAVDWLQYLNYDWTALYESKFPEERFLTIAKRVCE
                     YPASLKAHSRVEKIYTDRKEMYEGKKLFDWGMAETMAYATLLDEGTHVRLSGEDAGRG
                     TFFHRHAVIHNQNDGTGYVPLTQLHANQGRFQVWDSVLSEEAVLAFEYGFATTDPKTL
                     TIWEAQFGDFANGAQIVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARVERY
                     LQLCAQQNIQVCVPSTPAQVYHMLRRQAIRKMRRPLVAITPKSLLRHPLAVSSLDELI
                     NGSFQNVIGELDNIDPKQVKRVVMCSGKVYYDLIEKRRENNQTDVAIIRIEQLYPFPL
                     EEMQQILEPYAHVKDFVWCQEEPLNQGAWYCSKHNFEASLPEDAKLTYAGRPASASPA
                     VGYPSLHAKQQKQLVEDALTIAE"
     gene            19807..21036
                     /gene="odhB"
                     /locus_tag="PWY43_000546"
     CDS             19807..21036
                     /gene="odhB"
                     /locus_tag="PWY43_000546"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDL2135454.1"
                     /translation="MTIEILTPDLPESVADATVATWHKKVGDNVKRDEILVEIETDKV
                     VLEVPASSDGILAEITQEQGATVVSKQSLGKLVVAKAGDISSATIEQKTESTPSDRKH
                     AAIENSHANADDQGPAIRRLLAEHDLQAADIQGSGVGGRITREDIEKEIAKRVQINQV
                     KGSATSQNTVSTVAFNSRSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNELDMQPIM
                     NLRKTYGEKFEKQHGVRLGFMSFYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIA
                     VSTPRGLVTPVLRDCDKMSMADIEKKIKELAEKGRDGKLTVEDLTGGNFTITNGGVFG
                     SLMSTPIINPPQSAILGMHAIKDRPVAIDGQVVIRPMMYLALSYDHRLIDGKESVGFL
                     VAVKELLEDPTRLLLEI"
     gene            21329..22486
                     /gene="sucC"
                     /locus_tag="PWY43_000547"
     CDS             21329..22486
                     /gene="sucC"
                     /locus_tag="PWY43_000547"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDL2135455.1"
                     /translation="MNLHEYQAKQIFKQYGLPVSQGIACKTAEEAVEAIKQLQGEQWA
                     IKCQIHAGGRGKAGGVKLVRNEAEVREFADKWLGKRLVTFQTDANGQPVNTLYLEETC
                     NIDRELYLGAVVDRSAQKVIFMASSAGGMNIEEVAEKTPELIHKAVIDPLTGGQGYQG
                     RELAFKLGLKGDEVKQFADIFVKLANLFVEKDLALLEVNPLVITKEGQLLCLDAKMSI
                     DSNALYRHPELKELQDPSQDDEREAEAEKWNLNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLYGGKPANFLDVGGGATKERVAAAFKLILSDPNVKAVLVNIFGGIVRCDLIADGVIA
                     AVNEVVNVPVIVRLEGTNAAQGREILANSGLSILAASSLKQAAELAVKSAN"
     gene            22495..23364
                     /gene="sucD"
                     /locus_tag="PWY43_000548"
     CDS             22495..23364
                     /gene="sucD"
                     /locus_tag="PWY43_000548"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDL2135456.1"
                     /translation="MSILIDKNTKVICQGFTGGQGTFHSEQALAYGTQLVGGVSPNKG
                     GTTHLGLPVFNTVREAVEATGATATVIYVPAPGCKDAILEAIDAGIQLIVCITEGIPT
                     LDMLQVKQRLNQTGVRMIGPNCPGVITPDECKIGIMPGNIHKKGNIGIVSRSGTLTYE
                     AVKQTTDEGFGQSTCVGIGGDPIPGSNFIDILKLFQDDPETEAIVMIGEIGGSAEEEA
                     AAFIKENVTKPVVSYIAGVTAPKGKRMGHAGAIISGGKGTADEKFKILEDAGVTTVRS
                     LAEIGSALRKILG"
     gene            complement(23435..24880)
                     /locus_tag="PWY43_000549"
     CDS             complement(23435..24880)
                     /locus_tag="PWY43_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL2135457.1"
                     /translation="MDSIILFFESVLTWIVNTIDGPLWDVTIILLLGAGLFFTLTTGF
                     VQLRLLPQSLREMWGGRSTEGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAV
                     FWMWITALMGMSSAFAESSLAQLYKTKDPNGMFRGGPAYYITRGLKLPSLAIAFAVTL
                     IFTFGFAFNAVQSNSIVEATKNAWDWNPVYVGMTLVVITGLIISGGVKRIGKVSAQVV
                     PMMALFYLIIAVIILGMNIEKVPSIISLIIESAFDFSAAAGGMFGALVSKTMLMGIKR
                     GLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTIVVCTCTAVIILMSNNYGG
                     ETLRGISLTQSALQFHLGEFGLHFLAFVILLFCFTSIIGNYAYAETNIRYIKNSPWFV
                     WGFRAIVLFFVYFGAVRDGGIVWAFADTVMASMAVINLVAILILAPIVWIILKDYLRQ
                     VKSGKEPVFRIEEHQDLIHKGVDPLIWKSKE"
     gene            complement(24985..25971)
                     /locus_tag="PWY43_000550"
     CDS             complement(24985..25971)
                     /locus_tag="PWY43_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HDL2135458.1"
                     /translation="MRLKATYWGMIVIGFLSLFALYQLYPMILFKVMEWQKNFNLQLS
                     ASLNELAENQSKAGITLVFISFLYGVFHAVGPGHGKFILSSYLSFEKTKLNQAMKITF
                     ASALVQGLVAISLVTVIVVIFTLSRQYFNLALKWVERGSFGLMILFGIYWCYQVYRTV
                     NRARKPIIKSIRISQKKAGKLPLVTQKGHIHTENCSCRHKHLPTSNEMQQVRDWKAQL
                     MLVLSIGSRPCSGAILVLFLSYTLNLYAWGVISALAMAVGTGLTLSLFAYLVIIARNK
                     AVKVSQWYFSAQINRNIVWILKFTLGLALILFGMVLFHSSFIDAGTGVFFKR"
     gene            complement(25962..26600)
                     /locus_tag="PWY43_000551"
     CDS             complement(25962..26600)
                     /locus_tag="PWY43_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HDL2135459.1"
                     /translation="MKFIKTIITLIALYSMPVFSHPHSFVDLKTNVIVEGSMLKSFQM
                     EWMLDEIASSELIYEVKNSQDKEKTQQNITAEMVQTALQNHYFSTLYDSQNQPIKFTT
                     KPTATRFEIKNNRVIFYITFHLAKPHELKQSKVRFYTYEPSYYIAMEYNRPADVNISN
                     ASCKATLVQPQVDSKLRLYASGLDKNQSPDMPENGDYSLGVQFAQKVEIICD"
     gene            complement(26661..27039)
                     /gene="rnpB"
                     /locus_tag="PWY43_000552"
     ncRNA           complement(26661..27039)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWY43_000552"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         8498 a         5929 c         4885 g         8318 t
ORIGIN      
        1 caaattctga ttcatactgg aaagcaagcg ataaacctgc aactgcgtgg ttgattccat
       61 aatattagtc ggctcatctg ccaccaataa tcggggctga tttgccaccg ccattgcaat
      121 catcactttc tgagcttcgc cttcggtaat atcgcttggg taacttttca taatatcttg
      181 atgatcacga ataccaacac ggtgtagtag ctcgattgct tttttctttt tccacccaaa
      241 tcgttgccac cacttccctc taaattgaat ggtttgcatc agttgtttac caatttgctg
      301 gcttgggtca aggctagtaa gcggatcttg gaataccatt gaaatatgat cgcctaccaa
      361 ttttcggcgt tccgctggcg aaagtttcag taattcaata tcgtcaaagc gaaagcgatc
      421 tgcactcaca atccattcat ctttaatcat accgcaaatc actttggcta tgaggctttt
      481 tcccgaacca gattctccca ccaagccaca aatttcgccc tcatcaacag tcaggttgat
      541 gttatccacc atttttactc tgccttttgg cgtatcaatt tcaatactta aatggcgaat
      601 atcaagtaat gccatactat tccttaatct gtaaaatttt acttaaaaat gaccgcttgt
      661 tatgtgtcag tatcctattt aaatactatt ctttacggta tttttcaaaa atcacgctta
      721 agcgtgtacc aagtattgta acggtaaaaa tactaaaaat aattgctaag ccaggtaata
      781 tcaccgtcca aggagctata taaatcagtt ccatggattc acgaatcatc gctccccatt
      841 caggggtcgg gcttttagcc cccagcataa taaaacttaa cgcactaata tctaatatgg
      901 cgatcacaaa aatatgtgaa agtgctttaa ttgccacgcc ggtaagatta ggaataatca
      961 cttctcgaat taaataccaa tggcttgcac cgtctagctt taaggtaatg atgtactctt
     1021 ttttgagttg tttttcggtt gcttgataaa ttttatggat aaaatgcggc aacatcgcta
     1081 agaaaatcgc caacattgca ttgaccaaac tcgcttccat taaggttgca atgatgatcg
     1141 caatcaataa aatcggcata aataaaaagg tatcgaacaa atggctaatc aaccaaaatg
     1201 aacgtccttt tttcagcccg gcataaatcc cgatcatacc gccgataatc gcaattaata
     1261 aggtaatcat cagtgcggaa ccaagcgtgt agtaaaaacc atacataata cggcttaaaa
     1321 tatcccgtcc taaatcgtca gtgcctaaaa aatggctgat ttgacctttg tcgtcccaag
     1381 agggaggcat taattctaaa ccaacaaatt gttcattata attgtaagga gcaagcaaac
     1441 taccaacaaa aatgagagcc agtaatagca aaaaaagata aaaactgcca agtgcaagtt
     1501 tatcttgtcg gaaatgaaac caaaatgcct taaatgcgtt ggtttctcta aattgctcgg
     1561 gttctttaat aactgccata ccaatccttt ttatctgatg ggtctaacat cacccgcaca
     1621 aagcctgtaa ccatatctac taataacaca aacaccccga ttgcaactac tcccgctgaa
     1681 atcgcattat agtcttgaat agcaagggca tttatcagcc aacgtccaat tcctccccag
     1741 ctaaatatgt tctcaattaa cataccgaag gcaaaaatta aaataaaggt acgggcaatc
     1801 ataggtaata atgcaggtaa agtattacaa ataatatgcg ttctccaaac tctaaacggt
     1861 gtccagccac gcgttttcgc tattttaatg taattttgtt tcattacata acctgctcta
     1921 gcttgagtaa tatgcataac ttctaatgta gctggcacag ccaagactaa tgtcggcaaa
     1981 gctaagtgat gtaaagcact ctgcatcatt tttaatttat aaggtacatc agataaccaa
     2041 atatctaata gcaaaaagcc ggttaccatc tgtttcggat aaagcgaatg gatttctcca
     2101 attgctgaca cttcccactg attgagagaa gcgtaataca ttagcactaa tgccagccaa
     2161 aataccggca cggctaaact caatgaacct agtgtattta ataatcgccc aaccatattt
     2221 ttttgctgag tagccgcaaa aaaaccaaga ggcaaaccaa taattaaaga aacaatgaga
     2281 gccgataaac aaagcgtaat tgtagccagg aacacctgta aaatttgcgc tgtgagcggt
     2341 tctccattac tgaaactaat accaaaatca ccatttagca gagattttac ataataacaa
     2401 taagcctcaa accagcctaa ctcggctaag tggtttaatg gatctcgtaa cagaatatta
     2461 aaactgataa gtgacaaaaa aatcaacgta atcaaggtta gaaaaatttt tcgaaaaaag
     2521 acaatcagca taattagttt ccttttttat gccccaagcc caaacgtaac tctgataatt
     2581 ttacctggcc gaaaggggaa atatctgcct gtttcactcc atcttttatg actaatacac
     2641 gattagcatt cattaaaggg agaataggca attgctcttc taataatttt tgtgcaaatt
     2701 catacaataa attagctgat gccggatcat cacttagacg ggctgtttgt aaccaaaaat
     2761 caaaatcttc attgcaccaa tttgataaat tagtcaccgt ttccgcagct ttacaactga
     2821 gtaaggcctc caaaaaactg ttaggatcaa gattatttgc taaccaacca cctaaaatca
     2881 aatcataatc cgcactttta ttagcaagct gttgcaccaa ataagctcga cttactgcgc
     2941 gaatttcgac ctctaaattt tgcgtagcta aatcagcacg tatcatttct gccattttta
     3001 atgggtgttg attataaact cgtttttcat caattaccca cagttttaag cgctgttttt
     3061 ggtttacttc ttcggtctct gttttgctat ctaaagaccg atttataata ctgtcatgca
     3121 tttcaaccgc ttgctcacct gaattttggt gtggttgata agggtagctt ccattatttt
     3181 ttttcgacca caaagctgtt ggtaataggt tatccgctac ttcacctgta ccataaaata
     3241 atttctctgc aatacgtttg cgatttatgc cgcgtgcaat acgttggcgt aatgccaaat
     3301 cctgcccaat accccgttgc atattgaatg ctaaataaac caaattagca cctttactac
     3361 taataagctg ccgattattt aacactgata attggctagg ttccggaaaa gcaaccacat
     3421 cacattcatt atttaaaaat tttgccatcc tgccggtcgc ttctgtggaa aaatctacga
     3481 ccatattttg aatatatgct tttttgcccc agtaattcat atttggtttt aaacgcacat
     3541 aatcattatt ttcataactt tcaagttgat atacgcctgt tcctaccggt aataaatcca
     3601 gttgaactaa gttatcatct gcattgagct gtaaagcata ttctttagac ataataactg
     3661 catattggct ggctaaatgc gccaacaatg ctgaatcagg acggactaat tcaattttta
     3721 caatgtcatt tgacggagaa tgaatgctgg caattttatt cgctaaatca acactttcaa
     3781 aataaggaaa ataaatctgc cccgctgttt tttggtaaat atagctttgt cgctggtggt
     3841 gtaatacact ttcaggctca cttttcactt caggtaaatc actttcttta ccaatcattc
     3901 gttttagcga aaacaccaca tcttctgcgt ttaatttacg actcggagta aaccatggag
     3961 ttgtatgaaa agcgacattt tttcttaaac gtaatgtaat cactaaacca tcgtcgctga
     4021 ttgaataact ttcagctaaa gcaggctcta gtcgattttt tttcggatta aactcaacta
     4081 atttatcgta gatctgctct gttaccacat tcatattcga actcacatca gatttttgtg
     4141 ggttaaaaga gaatcccgat gaagtagtgc aatacacaag gctattttca agtagctctt
     4201 gaggaatact tggcgctgcg taagcggtcg aatttagccc taaatttgca aaacatactg
     4261 aaaaaatgac cgctagttta ttcatcttta ctgtttccat tttttgttaa cccatattga
     4321 cgcaatttgt ttgccaccgc agtatgtgaa agtccgagcc gttgtgctaa ttttcgggta
     4381 ctcggatatt ctgcataaaa ttggtgcaat aattgggctt catactggct catcatttcc
     4441 tcaagtgtcg cctcctcacg tacttcaatc atcgcctcca tatctctggg taaatgaaga
     4501 tcttttaccg ctaaacggta gctatgcgaa agtgtacaag ctcgataaag tgcgttataa
     4561 agctctctta agttgcctcg ccagtgataa ttctgcaatg cttgcaaaaa aacattatca
     4621 tactctaatt tgctcacccc caactgattg ctaatttggg cgataaagta atcagctaat
     4681 aatggaatat cctcttttcg atctcgcaaa ggtggtaaat ttaaagtgag aacatttaaa
     4741 cgatgaaaca gatcttctct tactttgcct ttttcaacta ataatgacaa aggctgtaat
     4801 gaagtacaga tcaccctaac attgacttga atctcttgat cttcgcccac tcgacgaaaa
     4861 gagccatcat ttaaaaagcg caataatttg gcttgcattt ccagcgataa ctcagaaata
     4921 gagtctaata atactgtacc accgtgagca tattcaaaga aaccgacgga ctcaacgcca
     4981 ttgccacgat gtccgaacat ttccgattct gcttcttcag ccggtaaacc ggcacagttc
     5041 acggcaataa atttttgcat tcgacgagat ccaaaatggt gacaagattt ggcaaataaa
     5101 tcttttcctg ttcccgtctc accttgaatc aataatggtg catcaaggtt ggcaaatttt
     5161 tttgcttgtt caacaacctc ttttatcttt acagattgtc caattatctg ttcaaaagat
     5221 tctggattta cgaccatata gtctcctata aatagcaaat ttgtgcattg tttaatacgc
     5281 ttaaagcctg atattagcta ctagagttaa caatgcgaga gggattgtga ggcgatcata
     5341 ccgctatttt tctcttttgg aaacttttgt ttacaaccct attcagatta tttaagttat
     5401 taggtttaca atacagcata gatcttctaa acttattgca ctttttatgc ctcaagcggt
     5461 cataaaaaac gaatttttta ctcgctaccc ccattggtaa agccgatgtt taaaggtaag
     5521 cgttttgcaa acaaaggagc aaatgatgta tttagaacaa attaaagctg agcttcaaga
     5581 agctgcagat gtattagaca aatttatgtc tgatgaaaaa aatattaaat taattcaaga
     5641 tgccgccatg ctgattgcaa atagtttcaa gcaaggtggt aaggtgattt cttgcggtaa
     5701 tggcggttcg cactgtgatg cgatgcactt tgccgaagaa ttaacaggtc gttaccgtga
     5761 aaaccgtccc ggatacccgg caatcgctat ttccgatgtc agccatttaa gctgtgtaag
     5821 taatgacttc ggttatgaat atgtcttctc tcgttatcta gaggccgttg ggcaaaaagg
     5881 cgatgtactc tttggtctat caacctctgg taactctaaa aatgtgttaa atgcgatcaa
     5941 agttgccaaa gaaaaaggca tgaaagtgat tgcaatgacc ggcaaagatg gcgggcaaat
     6001 ggcaggatta gccgatgtgg aaatccgagt acctcatttc cgttacgcag accgcatcca
     6061 agaaattcac attaaagtga ttcatatttt gatgatgtta attgaatttg agatggaaaa
     6121 agcctaatct ctataacaaa aaggcgtttg caccaaacgc ctttttctca tacttcatca
     6181 ataacaaact caaaccaatc aatcacatct ttttcgtgaa taccgttagc aatcagcatt
     6241 ccggcatttt ttacagaatc aggatcttta gagattaaag ggtgccaatc aaaaagaggc
     6301 tttccttcat aaagtaaacg ataagcacag gtttttggca accagccaaa atcaggtaag
     6361 ttctttttag ttaattttgt acaatccgac tcaattttaa aacggttcga gtaattcata
     6421 caacggcctg tattcacatc taataaatta caggcaaccg aggtatagta aagccgttca
     6481 cgccgccctc ttccctgaat atatttccgg tagcagcact taccacaacc gtcacataat
     6541 gcttcccatt cagcatcatt catttcaagt agcgatttgt tctgccagaa atttgcttca
     6601 aagtgttgtt caaactgcat aaattaacgt attacaaccg cagtaccaat cgctaccacg
     6661 atgaacattc ctttatcgcc gcccattgat tgatactcca tagaaacgcc aaccaccgca
     6721 tttgccccca ctttttgtgc ctccttagct aattcagcta atgcctcttt tcttgcaccg
     6781 tttaatttgc tttcataagc acctgaacgt ccgccaataa tatcggtgat accggctaag
     6841 aaatcacgga taaaattagc cccagaaact acttcgccaa atactaaccc tttgtattct
     6901 acaatttgtt taccctctat cgtaggtgta gttgtaataa tcatttattc tctcctgcta
     6961 attgctgttt taattgctct agtgcgattg cacggtgtga aatcgctttt ttctctttgg
     7021 tttctaattc ggcaaaagtg caagctttcg gtgggtagaa aaacagggaa tcgtagccga
     7081 aaccattttc acctctttct tccgtcaaaa tttcaccgaa acattcaccc gacgcaatat
     7141 aaggtgttgg gtcagtttcg tgttttaaaa acacgataca actgacaaat ttcgccagac
     7201 gattctcttg cccttgcatt ttggctagta atttttggcg attatcggca tcggttgcct
     7261 gctctccggc ataacgtgca gaatatagcc ccggttcacc accaagtgcc ataaccgata
     7321 aacctgaatc atcggcaatc gccgataaac ttgtcatttt agcagcaaat cgtgctttaa
     7381 tcagcgcatt ttcaacaaaa gtcaaaccgg tttcttccgg tgattcaatc ccaaattcac
     7441 tttgagccac cacctcaaaa ccaaattgtg agagtacatc tgccatttct ttaactttat
     7501 ctgcattact tgttgcaagt acaatttttg tcttttccat attgtcctta tttgcaaaaa
     7561 tattctaaaa aatcaccgct tgttattcga tacctttttg gtaagacatc atcagcaacg
     7621 ttcgccattt atcctcacct ttctcatctt ttcccatatt agcaatattc ggaaatcttc
     7681 cccattgttc aaagccgtgt tttctcatta gattttggct cactgtattt aattcaaaca
     7741 catacgccat caacgtatga atattgtgat taagcatttc agcttgcata aactcaataa
     7801 tttttgagcc atagcctttt cctttggaat agcggtctaa atagatactg atttccgaag
     7861 tatgtaaaaa tgccggtctt tcataaaatg gtgaaaaact aaaccagcca gcaatgcctt
     7921 gttcatcttc gaccacccaa ataggatatt gggctgtttc aaggtgaaaa tcgaaccact
     7981 tccgccgatt ttgcgtagtt gctagttcta aatcggcggt aatttggtga gtgggaattg
     8041 cttgattata aattgccaat attgtctcaa aatccgcttc tactgctttt cggaattgca
     8101 tttgttatcc ttttattcgt tgccaataat acgcaaaaaa ttaatatttt attaccgctt
     8161 gctaatcata ctcggcaata aaacctgtac ttttactccg ccactttcca cattttcaat
     8221 cgaaaattcg gctttgagtt cagccgcccg ttcttgcata atcgtcagtc cataatgccc
     8281 ttctggttgc gtatgggcag caagccctac gccattatct tgaatagtca aacaatgttc
     8341 accatcatca tttgtttcag caatcacatt aatttcagtg gcttgcgaat gcttaatagc
     8401 attaatcaca gcttctcgta caatttgcaa tacgtgaact tgctgctgag cattaaacat
     8461 atgcgaaggt aatttgcaac ttaagcgaat ctgtgctgag gttttcggtc ttaagctttc
     8521 taaaattttt tctaacgctt gttgtaaatt cgcttcttga atcgttagcc taaacgtcgt
     8581 caacagctcc cttaattggc tgtaagcatc atttaacgct ttttcaaata cagttaaaat
     8641 ttcattacgc ttttcatctg ttttagcaag cttaagcagg ctaacctgaa ttttgaaaaa
     8701 cgtaagagac tgggctaagg aatcgtgtaa ctcacgagca ataattgcac gctcctccat
     8761 gagtaccaat tgttgctgtt gcttttgcat ctgcactaca taaaggctac gaccaatcat
     8821 ttccgacaca ttttggatca atcgtccatc aggacaaacc agtgagggtt tccagcttaa
     8881 gatacccaat ttttcatttt ctattgcaat ttcagcagaa acccaatcta aacttggttt
     8941 attgtctatt gatacattcc aatattcagc cccatatact tgtaaatcaa tccctagcaa
     9001 atgttcatta tcaaataccg ttttaagtac ttgatttaac acttctttat taatcggctt
     9061 agcagttaat aattgagagc attgatataa taccagtaaa gaacgattta ccgccaataa
     9121 acgacgtgtt ttatcctgag ctttatcttc taatgaagta tataatttta ataattcatt
     9181 agacatctca gtaaaagtag aggaaagtag ccctaattca ttgggttcat taaccgctaa
     9241 ttttacgtgg tcaaaatctt tattttgaat ttgccaactc gcttttacca attgccttaa
     9301 aggtccaata atcttttttc gagtatacca aacgccaata taagcaaggg caataatcag
     9361 taacattgca atcgcaatca cgcctgttgc aatctttaat tttaactcag cgaactcttg
     9421 taagcggaaa acaaaatcat caactctctg cacataatct tcaatgtgcg aagcataatt
     9481 aactttatct ctctgagcga cataagattg catttcatac caatctttaa gtaattgttg
     9541 atagctttct atcacgctat ctgacaataa aacactttgc tttaatgaac taagctctgt
     9601 agcatgtagc gttcgatgat attccaacaa tcgctcaggt agcagcgctt tttttgaatc
     9661 catttctgat aacaatcgat aactttgcat acgaagtgag cctgaaacat taatcaggct
     9721 cgcatcagat ttattgctcc acataatacc aagtgagatt ccactgataa tcgaagcaaa
     9781 tccgatcata attaaaaaat agagtcctat ccgcctagca atagagtatt ttgcttttat
     9841 cataattaaa gattaaattg agctttcaat tcattagcaa tactatcttc attatttgaa
     9901 cctatagttt tcttcgcttt ttgctgaata tctggttctg aatttcccat tgcaatgcct
     9961 aatccaacgg tttctagcat agaaacatca ttgaaattat cgccaaaggc aatcaccttt
    10021 tgtaaatcaa taccttctgt ttttaatact tccgctaatc ttgccccttt gctgttacca
    10081 acactggtaa tatcaaccct atcaacccaa gaccattccg ggcttacttg atcaggcggt
    10141 aatttttcaa caaatttttg cattttggca agatctggat cgctaataag cactttccat
    10201 acggttacac ctttatcaat ttcttgttgg aaattttcta cctgataaac attcggacga
    10261 acttctggtg cgcaagactc aacccatttt tggaatttag tgaagtgaat attcaaagac
    10321 tcataagtca ttgcatcttc aaaataaacc gcagtatgaa tcccttgagc ttgtgcatca
    10381 ttaactaatt ttgaagcaat ttctgctgat aacggattac cgcttaatac tttttcattt
    10441 ttaaaatcat acacataagt gccattgcaa cacacaactg gcgtatccag ccctagttct
    10501 acataataag ggcgaaccgc agtatggtgc ctaccggtaa caaagtagac tttaattccc
    10561 ttttctctta cctttttaat agcctcttta ctggattcta aaattgttgc gttcgatgaa
    10621 agtagtgttc cgtctaaatc aaatgcaata gcttggtagt tcatagtatt cccctataaa
    10681 taaatagata aaagaaaagg caaccatttg gttgccttaa tattctactc taagttagtc
    10741 aaaactagaa attatttact gccatcatat gctggtgttt gataaagtgg agccggacca
    10801 ttaggacctg caacattgcc accttcattt tgtttcatta caccaccatt tgcagaaatt
    10861 tcaacacgac ggttagggcg taaacaatct tttaattctt gaccagtttc accatcacaa
    10921 gcttttactt gattcgcttt accataacct accgcttcaa tttgtgctgt aacgccttgt
    10981 tgtactaaac gagctttaac tgtattagaa cgacgctgtg ataagtctaa gttataagca
    11041 gcagagccta aacggtctgt atagcctgct actttaactt gttgtgcttt agatgctttt
    11101 aattgttgag caacattatc aacaacttct ttaccttttg tagttaaagt cgctttatcg
    11161 aaatcgaata agaaatcgcc agttaaagta aatgatgagt ttccattaca tccatttgga
    11221 taccagaaga aactttgtgc attcatattt ttatcgaata aaattttaaa ttggcaaatt
    11281 ttatgtacgc cattttcacg gtaattgaat gcatagtccc attcacgaac accatataag
    11341 ccttcttcaa aatgaagacg accaattaga ttatataatt gatctttgtt catccctttt
    11401 tcaatttgac gaacgttttc ccagtttggc catgaaccaa attgtgaacc atcgtggtta
    11461 aattcagatt ctgagatttt cgggaatacc gggttttcag ttgttccttc atcactcact
    11521 ttacttaagt taccacaagc agccactgct aatgccactg tcgataataa aaatccacgg
    11581 aaaagggctt tttttccatt ttcatattaa gtttccttat agtaatctat ataaaaaaat
    11641 agcttcacaa cctattaaag tcgcattcgg acggattcta caatacaaat ataattaatt
    11701 ctattaaatt tacgtaaaat aacatttcaa atatatttaa gatactccac ttttaattgc
    11761 aaagatttct ctcctcgaaa ttcattaata tccaacctat aaactaatct tgcctttttg
    11821 atcgataagt caggaaacgc ccttacatcc acattaaacc aaattgcatc aaatagttgc
    11881 cctttctctg attgcaacat taattttaaa tgcttacctg cgagtaattt ttgttggaga
    11941 atgctaaact ccccttcaaa actaggttct ggaaaatgtt gtccccaagg cccaccttgc
    12001 ctaatcatct cggcattggc taaagtaaac tcttgtgcat ttaactcacc atcggtataa
    12061 atgatgcctt gtaattgctc aggctcgata attgcattaa tagattggtc aaatatctgt
    12121 ttaaataaat ctaattttga ttgatggata gtcaaaccag ccgccattgc gtgtccacca
    12181 aatttttcaa tcaagtctgg atgtcgcata tcgatatgtt ccagtaaatc tcgcatatgt
    12241 actccgttga tcgagcgagc agaacctttt agatattcac tttcctcact ttcttgtgca
    12301 aaagcaatga caggacggtt aaattgatct ttaatgcgag aagctaagat cccaattaca
    12361 ccttggtgcc aatctgcttg atataacact attccgtgtg cctgttcaga ctgcgcaaga
    12421 cttggcaaat tcgcacaaat cgacagtgct tctgctttca tttcttgttc aaactctttt
    12481 cgggtttgat taagagaatc aagatcaagc gcaagccggc gagctaattc catattttca
    12541 caaattagta actcaacccc taaagacata ttttctaacc gaccagctgc attcaaacgt
    12601 ggtgcgatag caaaacccaa atctgctgct tgcaaagtag aaagatctcg cttgcccacc
    12661 tccgctaaag cttttatccc taaacggcaa tgccctgaac ggattcgctg taacccttga
    12721 tgcaccaata tccgattatt ttgatctaac ggcactacat cagccactgt gcctaaagcg
    12781 actaaatcca gtaattcagc aaaatttggc tctggtttat tttcaaataa atgttgttct
    12841 ctcatacgag aacgcaatgc catcattaca tagaagatca cgcctacccc ggctaaagat
    12901 ttagacggaa attcacaaaa agctaaattg gggtttacca gtgcatctgc attgggtagg
    12961 ctctctggcg gtaaatgatg atcggtaacc aaaacttgaa tctgatggct ttttagcaga
    13021 gcaatgcctt cagtcgaaga aattccatta tctaccgtta agactaaatc cgcccctttt
    13081 gctaatacca tttcagcaac cgctacactc aatccgtacc cctgagaaaa acgatccgga
    13141 attaaataat ctaccttatc gaaacccaat tggcgtaacg caataagggc aagcgatgta
    13201 ctggttgcac catctgcatc aaaatcaccc acaataatga tccggctttt ttcacgaaag
    13261 gcttgtaata gcaagtcaac ggcttgctca atattggcaa gttgctgtgg tctatgcaaa
    13321 ttctttaaat tatgctctag ttcttgcgta ttctttatgc ctcgactttg atagattcga
    13381 tttaataacg ggtttgtaga gactatttcc gttgttaata atgggcgcct tttaattatt
    13441 ttttccacaa tactcttctt taatgaaaca acaagcggtc ttcttttgca aaaattttac
    13501 aaaaaaagac cgcttgtatt caagttaatg aattactcac ttaacatttt cgctaattct
    13561 ttaggctcta cataaccacc aatcaactca ccttctgaag ttaccatatt tggggtacct
    13621 gtcacaccaa atttaatccc taattggtaa tgttttttca caatatccgg cgtttttatt
    13681 tgggttggta atttaccatc ttcagcatca ttcagtgctt ttactttgtc tgctgatgtc
    13741 caaatagctt ccatttgttt tgctgtttgg gtattcattc caccacgcgg gaatgccaaa
    13801 tagcgaatgg taatacctaa atcattatat tctttgattt tagtatgtag taaatggcaa
    13861 taatggcaag taatatccat aaacaccgta atcacatgtt tctcattttt cgctggatat
    13921 acgatcattt cattagtgta tgaattcaat tctgccaaca atccttgatt agtaatatca
    13981 atcgctttac cattttttag ctcaaatact ttaccttgaa ttacataacg accgtcttgg
    14041 ctgatatata caacaccttc atttgaagtc gccattttaa agcctgttaa tggagaatct
    14101 gaaattttaa catctgtggc tccaattttt tcaagctggc tttttagttt tgcatctgaa
    14161 gcaaacgtga tggttgataa acacattaat cctaaagtga ttagattttt tttcattgag
    14221 aatccttaca tttttaatct actttctaca ttcttcgctt tatttaagaa tgcttgacgt
    14281 tctttattac ccatacctga atttgagcca ggcaatttta ccgtaatcgg gttgaccggt
    14341 cgaccattaa tatggaattc atagtgtaaa tgaggcccgg ttgaaccgcc tgtattaccc
    14401 gataatgcta tccgctcccc ttttttaaca ctctgccctg gttttaccaa cgttttgctg
    14461 aggtgcatat aaacagtcgt aatatgtccg tgttttatcc gaatataacg tcctgctcca
    14521 cgggcttgat atgccacatg ctctacgaca ccatcacttg gtgcgatgat cggagtacct
    14581 attggcacgc catagtcaac ccctttatgc ggactaattc tccctgtaat agggtgtctg
    14641 cgtttggggt tgaaagaaga agttactctt gctgcaaata ataatggatg acgagcaaat
    14701 cctccactta atgtttcacc atatcgacta taatagcgtc cattatctgc ttgaatagca
    14761 taataacttt ttttactgct aattacatgg attccttcca catttcctaa atccgttact
    14821 ttgccgtcaa tatattcccg cttaatcaga attgcaaacc tatccccttt ttttaattta
    14881 ttcgtcgcaa tttgtgactg caatccaacc gctagctggt taatttggcg gtctgataag
    14941 ccaacggctt ttaagctaac cgcaaaacta ttctgaatag ttcctcttac aacatctaac
    15001 ttccattccc cttttttctc aaatctttga aaagaaaatt taccattttc ttttcgctca
    15061 taaacccgtt cctctttttc ggaaaccagc cagttcatat attctaattc accgtcatta
    15121 tctaataccc aataaaattg ttgacccggt tctaattgtg ctaatgccga atgttgctta
    15181 attaaatttt tcgcaacgga tgcagttaag cctgattgct ctaatacatc tttaagttta
    15241 tcgcctcgag ctacggtata actgaattgg ttttgaattc gtagtgcttg atcagccaca
    15301 tcaagtaaat cttctaatgc cttttcagct tcttctggga gtttttcccc aggaacttga
    15361 ttttcaaccg tttcaatccc ttcttctact tcatcgtctt gcccaactaa ttcatcttca
    15421 taagaagtcg ctgtttgatc atcaacaatt tcgtcgtcat gttcgattac taatgccgac
    15481 tgagtcagta aatcagtcac ataattaata aaactggaat gttgctcctg atggagagta
    15541 ttattttccg ccaaattagt gttgatactc tgatattttt cctgtttatc aatggcaata
    15601 atatcttcct gtgaagatgt attattgatt gaggattgat cttttagtgc gagaacaata
    15661 ccaacaaata gactaaatac cgctaataag aaaaccgcaa tcttaaaatg ttttttatgt
    15721 cgcttacgat ctctcgctaa aattacatgt tgcaaaatat atcccgctat ttttaaaatt
    15781 tactaaaatt tacatatttt tctgagtaga ctattttacc aagaaaaaat tcatttaatc
    15841 tattgtcaca acacgaaaaa tcagctaatt ttaactcaat acccctaatt tttttatatc
    15901 aatgaaaatc catcatctta aaccgaatct aaataccact acatttgaac tattcaaaca
    15961 gaatttatcc gcgacatcac tggtttctct tgagaatatt gaagtgtctt tcaatggaca
    16021 aaaagcatta caaaatatta atttatctat ttatcctaac tctatcacga cgatagtcgg
    16081 gccaaatggt ggaggaaaat ctaccctgct taaaattttg ctaaaattac ttagcccaaa
    16141 taaaggaaag gttattcatc aaaaaggact caaaattggc tatgtaccac aaaaattgca
    16201 tttagaaccc tcgatgccca ttactgttta tcgatttctt gcgctaaaac cacatactac
    16261 caaagaaatg attaaccaag cactagatct gttttctatt acccatctca ctgaaaatag
    16321 tatgcaaaaa ttatcgggtg gtgaacttca acgtgtattg ctggctcggg caattttaga
    16381 tcgtccgcaa cttttagtgt tagatgaacc aatgcaaggc gttgatatta taggccaaac
    16441 cgaactctat caattactta ataaaacaag ggatctcctt aactgtgcta ttttaatggt
    16501 ctcacacgac ttaaatattg tgatggcgaa tacagatgaa gtgctttgca tcaataaaca
    16561 tatttgttgt gccggaagcc ccgaagtcat tacacacgat ccgagcttta tccatttttt
    16621 tggcgaccaa tttgcacgta atgttgcatt ttattcacat aaacataacc atcaccataa
    16681 tttacatggc gatatctgcc ttggtgaatg tagttgtgag cactgatagg cgtccttttt
    16741 accaaatgtt taaaatttgt aaaaataatg ctaaaaataa ccgcttgcag cacaagattt
    16801 atctaaaaat cagacttact tcacatttta tttgcttaaa ttggagtata ataagggcgt
    16861 gatttcatac cacaaacagg tcaggtcttt atgcaatata cacattttga tgattggctt
    16921 gcatcatctg cattaggcgg tgcaaaccaa tcttatatag aagaaattta tgaacagtat
    16981 ctagaaaatc cagcaaatgt tgatgaaagc tggagaaaca tctttgattc atttcctaaa
    17041 actccccaat ttgaacaagc tcacacccct attcgtgact atttccgtaa attagcccgt
    17101 gaacagcaac ctcaatccgt cactattatt gacccagaag caggggcgaa acaagttcgc
    17161 ttactgcaat ggattaacgc tcaccgtttc cgtggctatt tagaggcaaa actagaccca
    17221 attaactatt accgttggaa aacctccagc gtgcctgaat tggactacaa atatcacggc
    17281 ttcaccgatg aagatctcaa cgaaactttt acggtcggtc gctatgtcta tgataaagac
    17341 acaatgaagc tcagcgaact ggataaagcc ctaaaagaaa cttatttagg gcctattggt
    17401 ttagaattta tgcacgttca agatattgaa caacgcaact ggcttcaagc caaaatggaa
    17461 agcgtgctaa acaaaccgct gttcaccaaa gcagaaaaac tgaatttatt aagcgaattg
    17521 accgctgccg atggtttaga gcgttactta ggggcaaaat tcccgggggc aaaacggttc
    17581 tcattggaag gcagtgatgc ctttattccg atgatgaaag aaattatccg ccacgcttcc
    17641 cgtcaaggta tgaccgatgt ggttttaggt atggcacacc gtggtcgttt gaatatgtta
    17701 gtgaacgtat ttggtaaacg cccggcagaa ttgtttgatg aatttgccgg caaacacgct
    17761 gatgaaagcc gcacgggcga tgtgaaatac caccaaggtt tctcctcaaa ctttgccgta
    17821 gatgataaaa acgttcacct tacccttgct ttcaacccat cgcatttaga gattgttagc
    17881 ccggtggtaa tcggctcagt gcgtgcaaga caagaacgta tgcgtgatac cgaacacgaa
    17941 aaggtgcttg cggtgaccgt tcacggtgac tctgcagtgg cagggcaagg tgtagtgcaa
    18001 gaaaccttaa atatggcaaa tgctcgtggc tataaagtag gaggcactat ccgtattgtg
    18061 atcaacaacc aaatcggatt taccacctct aacccaaatg acacccgctc caccgaattt
    18121 tgtaccgaca ttgccaaaat gattcaggca ccgattattc acgttaatgg tgatgatccg
    18181 gaagctgttg cctttgcagc gagaatggct gtggaatatc gtactaaatt taaacgtgat
    18241 atttttattg atttaatctc ttaccgtcgt cacggtcata atgaagccga tgagccttta
    18301 gcaactcagc cgatgatgta tagcatcatc aaaaaacacc cgacacctcg caaagtctat
    18361 gcggatcgtt taattaccga aggtgtttta accaacgagc aagaaatcga gatgttaaac
    18421 ctgtatcgtg atgccttaga caacggcgaa tgcgttgtgc cggaatggcg tgaaatggat
    18481 atggcagcgg tcgattggct acaatatctc aattacgact ggactgccct atacgaaagt
    18541 aaattccctg aagaacgatt cttaaccatt gccaaacggg tgtgcgaata tccggcttcg
    18601 cttaaagctc attctcgggt tgaaaagatc tacactgatc gcaaagagat gtatgaaggc
    18661 aagaaattat tcgactgggg tatggctgaa acaatggcat acgctacttt gttagacgaa
    18721 ggcacacatg ttcgcctttc aggagaagat gccggacgtg gcactttctt ccatcgccat
    18781 gcggtaatcc ataaccaaaa tgatggaaca ggctatgtgc cacttactca actgcacgcc
    18841 aatcaagggc gtttccaagt atgggattcc gtactctctg aagaagcggt actcgccttt
    18901 gaatatggct ttgccactac cgacccgaaa acactgacca tttgggaagc tcaatttgga
    18961 gatttcgcta acggagctca aattgttatc gaccaattta tcagctcagg cgaacaaaaa
    19021 tggggcagaa tgtgcggttt agtgatgtta ttaccacacg gctatgaagg tcaagggccg
    19081 gaacactcct ctgcccgagt ggaacgctat ttacagcttt gtgctcagca aaatattcaa
    19141 gtttgcgtgc catcaacacc ggcacaggtt taccatatgt tacgccgtca agcgatccgc
    19201 aaaatgcgtc gcccattggt tgcaatcaca ccaaaatcat tgctacgcca cccacttgcg
    19261 gtgtcatctc ttgatgaatt gattaacggt tcattccaaa atgtgattgg cgagctcgat
    19321 aatatcgatc ctaaacaagt caaacgtgtg gtgatgtgtt cgggtaaagt gtactacgac
    19381 ttaattgaaa aacgccgtga aaataaccaa acggacgttg ccattattcg tatcgaacag
    19441 ctctacccgt tcccacttga agaaatgcaa caaattctcg agccttacgc tcacgttaaa
    19501 gactttgtct ggtgtcaaga agagccacta aaccaaggtg cgtggtattg cagcaagcac
    19561 aattttgagg cttctctgcc ggaagatgca aaattaactt acgcaggcag accggcttct
    19621 gcttcaccgg cggtgggtta tccatcgtta cacgcaaaac aacaaaaaca acttgttgaa
    19681 gatgctttaa cgatcgctga ataataaaat gggcgtatat acgccctaca agcggttcac
    19741 ttttaaccaa attttacaat ttacgctgtc ggagttttaa aactctctcg aaaaaaggaa
    19801 taagttatga ccattgaaat tttaaccccc gatttacccg aatctgttgc cgatgcgacc
    19861 gttgccactt ggcataaaaa agtgggcgac aatgtaaaac gtgatgaaat tttagtcgaa
    19921 attgaaacag acaaagtcgt tttagaagta ccggcttcaa gcgatggtat tcttgcagaa
    19981 attacccaag aacaaggtgc tactgttgta tccaaacaat ctctgggtaa attagtagta
    20041 gctaaagccg gtgatattag ctctgcaact attgagcaaa aaacagaatc cacgccatca
    20101 gaccgcaaac acgcagcaat tgaaaacagc cacgcgaacg ctgatgatca agggcctgcg
    20161 attcgccgtt tattagccga acacgactta caagccgctg atattcaagg cagtggtgta
    20221 ggcggcagaa tcacccgcga agatattgaa aaagagattg ccaaacgagt gcaaatcaac
    20281 caagtaaaag ggtctgcaac cagccaaaac accgttagca ctgtagcatt taacagccgt
    20341 agcgaaaaac gtgtgccgat gacacgactc cgcaaacgta ttgccgaacg cttactagag
    20401 gcaaaaaaca ccactgcaat gctcactact tttaacgagc tggatatgca accaattatg
    20461 aatttacgca aaacctatgg cgaaaaattc gaaaaacaac acggtgtgcg tttaggcttt
    20521 atgtcgttct acattaaagc cgtggttgaa gcattaaaac gctaccctga aatcaatgcc
    20581 tccattgatg gcgatgatgt ggtttatcat aactatttcg acatcagcat tgcggtttct
    20641 accccacgag gcttagtcac acctgtgttg cgtgattgcg acaaaatgag tatggcagat
    20701 attgagaaga aaattaaaga acttgccgaa aaagggcgtg acggcaaatt aaccgttgaa
    20761 gacttaaccg gcggtaactt taccatcacc aacggcgggg tattcggttc attaatgtcc
    20821 accccaatta tcaacccgcc acaaagtgcg attttgggta tgcacgctat taaagaccgc
    20881 cctgtcgcca ttgacggaca agtagtgatc cgcccgatga tgtacctagc cttaagctat
    20941 gaccaccgtt taattgacgg taaagaatct gtcggtttct tagtggcagt taaagagtta
    21001 ttagaagacc caacccgtct tcttttggaa atttaatcga aaatatgacc gcttgtactt
    21061 ttagctacaa gcggtctgtt tttaataaaa tttgacaaaa tccgataaac gctctatttg
    21121 agttcccttc tgagccacct gagcgacttt gaatttgtag gaaaaattta gtgtttgagc
    21181 ttacaaggca agcgagttat aaatttttcc gtcaagcaaa ttcaaactaa gcgaagaaag
    21241 tggcgaagca gggtgtgctt tcttttggtt acttttcttt gcacaagcaa agaaaagtaa
    21301 cataaaaaaa accaaacgag gaaactctat gaatctacac gaatatcaag caaaacaaat
    21361 attcaaacaa tacgggcttc ccgtaagcca agggattgct tgcaaaacgg cagaggaagc
    21421 cgttgaggca attaaacaac tacaaggtga acaatgggcg ataaagtgcc aaattcacgc
    21481 tggtgggcgt ggcaaagccg gcggggtgaa actggttcgc aatgaagccg aagtgcgtga
    21541 atttgccgat aaatggctcg gcaaacgctt agttaccttc caaaccgatg ctaatgggca
    21601 accggttaat accttatatt tagaagaaac ctgcaatatc gaccgtgaat tgtacttagg
    21661 tgcagtggtt gatcgctccg ctcaaaaagt gatttttatg gcatcaagtg ccggtggtat
    21721 gaatattgaa gaagtagcgg agaaaacccc tgaattaatt cacaaagcgg taattgaccc
    21781 actcaccggt gggcagggtt atcaagggcg tgaattagca tttaaattag gcttgaaagg
    21841 tgatgaagtt aaacaatttg cggatatttt cgtgaaactg gctaacctat ttgttgaaaa
    21901 agatttagct ttattagagg taaatccact tgtcatcact aaagaagggc aactactctg
    21961 cttagatgca aaaatgtcga ttgacagcaa tgctctctac cgccacccag aacttaaaga
    22021 gttgcaagac ccatcgcaag atgacgaacg tgaagctgaa gcggaaaaat ggaatctcaa
    22081 ctacgttgca ctagatggca atatcggctg tatggtaaac ggtgcggggc tggcaatggg
    22141 gacgatggat atcgtcaaac tttatggtgg taaaccggct aacttcttag acgttggcgg
    22201 tggtgcaacc aaagagcgtg tagcagcagc ttttaaattg attttatctg atccgaatgt
    22261 gaaagccgtg ttagtcaata tttttggtgg tatcgtccgc tgcgatttga ttgctgatgg
    22321 cgtcattgct gctgtaaacg aagtcgtgaa tgtgcctgtt attgtgcgtt tagaaggcac
    22381 taatgccgca caggggcgtg agattttagc caatagcggg ctgagtattc tagcggcatc
    22441 atcattaaaa caagcggctg aattagcggt aaaatctgca aattaggagg aaaaatgtcg
    22501 attttaattg ataaaaacac aaaagtaatt tgccaaggtt tcaccggcgg acaaggtact
    22561 ttccacagcg aacaagccct tgcttatggc acacaattag tcggcggagt ttcgccgaat
    22621 aaaggtggca caacccattt aggcttaccg gttttcaaca ccgtacgcga agcggtagaa
    22681 gcaaccggtg caactgcaac cgttatctac gttccggctc ccggctgtaa agatgctatt
    22741 ttagaagcca ttgatgccgg catccaacta attgtctgta ttactgaagg cattccaacc
    22801 ttggatatgc tacaagttaa acaacgtcta aaccaaaccg gcgtgcgaat gatcggtccg
    22861 aactgcccgg gtgtgattac ccctgatgag tgcaaaatcg gcattatgcc gggcaatatc
    22921 cacaaaaaag gcaatatcgg tattgtttct cgttcgggta ctttaaccta tgaagcagtt
    22981 aaacaaacaa ctgacgaagg ctttggtcaa tctacttgtg tcggtatcgg tggcgatccg
    23041 attccgggat ctaactttat tgacatttta aaactgttcc aagacgatcc tgaaactgaa
    23101 gccattgtga tgattggtga aatcggcggc tctgccgaag aagaagcggc agctttcatc
    23161 aaagaaaatg tcaccaagcc tgtagtcagc tatattgccg gtgtcaccgc accaaaaggc
    23221 aaacgaatgg gacacgccgg tgcgattatc agtggcggca aaggaaccgc cgatgaaaaa
    23281 ttcaaaatcc ttgaagatgc cggtgtgaca accgtcagaa gtttagccga aatcggttca
    23341 gctcttagaa aaattttagg ctaaactaaa agttcaagcg gtcatattta cccatttttt
    23401 tgtaaatatg accgcttgtt tttttgctat taaattactc tttcgatttc caaattaatg
    23461 ggtcaacacc tttatggata aggtcttgat gttcttcaat tctaaacact ggctctttgc
    23521 cacttttcac ttggcgaaga tagtctttta aaataatcca gacaatcgga gcaagaatta
    23581 agatagccac caagttaatt accgccattg atgccatcac tgtatcggca aatgcccata
    23641 caatccctcc gtctcttact gcaccgaagt acacaaagaa taaaacgatg gcacggaatc
    23701 cccacacaaa ccacggactg tttttaatgt agcggatatt agtttctgca taagcatagt
    23761 taccgataat ggatgtgaag caaaacagta aaatcacaaa tgctaaaaag tgtaaaccaa
    23821 attcacctaa atggaattgt aacgcacttt gagtaagtga aatacctctt aaagtttcac
    23881 caccatagtt atttgacata agaatgatca ctgcagtaca agtacagact acaattgtat
    23941 ctacaaatac gcccaacatt tgaattaagc cttgacttgc cgggtgtttc gcatcagaag
    24001 tcgctgcaga atttggtgca gatcccatac cagcttcgtt tgagaacaag ccacgtttaa
    24061 tccccattag catagttttg gataccaatg ctccaaacat tccacctgca gcagcagaaa
    24121 aatcaaaggc gctttcaata attaaagaga ttatacttgg cactttttca atattcatac
    24181 ctaaaataat tactgcaata atcaagtaga ataatgccat cattggaaca acttgagcag
    24241 aaactttacc aatacgcttg acccctccag aaataattaa ccctgtaatc accactaatg
    24301 tcatgcctac gtagactggg ttccaatccc acgcattttt ggttgcttcc acaatagagt
    24361 tagattgcac agcattaaaa gcaaaaccaa aggtgaagat aagcgtcact gcaaaagcaa
    24421 tagcaagaga aggcagtttt aaaccacgag tgatgtaata ggctggccca ccacgaaaca
    24481 ttccgtttgg atctttggtt ttatacaact gagccagtga agactcagca aaggcggaac
    24541 tcattcccat taaggcggta atccacatcc agaataccgc acccggccca cccaaagcga
    24601 ttgcagtagc taccccacca atattaccaa ccccaacacg gctggctaat ccagtagtaa
    24661 aggcttggaa cggagttaat gatttccctt ccgtagaacg tcctccccac atttcacgta
    24721 aactttgcgg aagaagacgc agctggacaa aacctgttgt aagagtgaaa aataaccctg
    24781 caccgagcaa taggataatc gttacatccc aaagtggacc gtcgattgtg ttcacaatcc
    24841 aagtcagtac actctcaaaa aatagaataa ttgaatccat aaagacctct taattgaaaa
    24901 taaaacttcc acattgtaac cgatttaata tttttaaaaa gtttttttaa tacttttaat
    24961 aaaaattttt aataacttat taatttagcg tttgaaaaaa acgcctgtac cagcatcaat
    25021 gaaactactg tgaaataata ccattccaaa tagaattaat gcgagtccaa gtgtaaattt
    25081 taatatccaa acgatatttc gattaatttg agctgaaaaa taccattggc taaccttaac
    25141 tgccttattt ctcgcaatga taacaagata agcaaataag cttaaagtca gccctgttcc
    25201 tactgccata gctaatgctg aaataacccc ccaagcatag agattgagcg tataagaaag
    25261 aaataaaaca agaatagcgc cagagcaagg ccttgagcca atacttaata ccaacatcag
    25321 ctgtgctttc caatctctca cttgttgcat ttcatttgaa gtcggtaaat gtttatgcct
    25381 acagctacaa ttttcggtat gaatatgccc tttctgcgtg acaagcggta attttccagc
    25441 ttttttttgc gaaatacgaa tggatttaat gataggcttt cgcgctctgt tcaccgttcg
    25501 atacacctgg taacaccagt aaatgccgaa caagatcatc aaaccaaagc tcccccgctc
    25561 aacccatttt agggctaaat taaaatattg gcgagagaga gtaaaaatca ccacaatcac
    25621 cgtcaccaat gaaattgcca ctaagccctg caccaatgct gaggcaaaag tgattttcat
    25681 cgcctgattt aattttgttt tttcaaaaga aagataactg ctcagaataa attttccgtg
    25741 accaggccca actgcgtgaa atactccata taaaaaacta ataaatacta aggtaatacc
    25801 ggctttactt tgattttcag ctaattcatt gagcgaagcg gaaagctgta aattaaagtt
    25861 tttttgccat tccatcactt taaacaatat cattggataa agctgataaa gagcaaatag
    25921 gctcaaaaat cctatcacta tcattcccca atatgttgct tttaatcgca tataatctcc
    25981 actttttgag caaattggac accaagagag taatctccat tttcaggcat atccggtgat
    26041 tggtttttat ccaaaccgga agcatataag cgtaacttac tatctacttg aggttgcacc
    26101 aatgttgctt tacaacttgc attactgata ttgacatcag cagggcgatt atattccatc
    26161 gcaatataat aactcggttc ataagtatag aatctcactt tactctgctt taattcgtga
    26221 ggtttagcta aatgaaaagt aatataaaaa atcactctgt tattcttgat ttcaaagcga
    26281 gtcgctgtgg gctttgtcgt aaattttatc ggctgatttt gactatcata taaggtgcta
    26341 aaataatgat tttgcaatgc ggtttgcacc atttctgcag tgatattctg ctgagttttt
    26401 tctttatctt ggctattttt cacttcataa attaactcag aagaagcaat ttcatctagc
    26461 atccattcca tttggaaaga tttcaacatt gagccttcca caataacatt cgttttcaaa
    26521 tcaacaaatg aatgtgggtg agaaaaaact ggcattgaat ataatgcaat taacgtgata
    26581 atcgtcttaa taaatttcat atttactttc actataaaat aaaaaagcga acaagttccc
    26641 cctgttcgca tttcaagaat ttagtgagtt ggtcgataag ccgagttctg tcgtggacaa
    26701 tcattcctct agacgtacat ttacacatac gctcaagcaa cctacccgaa ctctcagcgg
    26761 gccaccgatt gagttcctat ttggtcttgc tacgagtgga gtttaccctg ctgtggaatg
    26821 ttaccaccca cacggtgcgc tcttaccgca ccctttcacc cttaccgaca aagccggcgg
    26881 tctgctctct gttgcactgg tcgtaggctc acgccccccg gacgttatcc ggcactctgc
    26941 cctgtgtagc tcggactttc ctctcgttta cttgtccccc agtttacgtt ttcacgaatt
    27001 tagaggcttc aataaaccag cgattgccta accaactcag cactagagta taatgctttc
    27061 gcaaaaaatt gcaaggaaat aaccgcttgt agttattatg gcaaaataaa aaaggattaa
    27121 gttcatttaa aaacttaatc ctcttcgctt taattcactc gtaaaaatta cgcacgtttg
    27181 aagtctaagt gaaccaatta aaaagcacct aaaattttcc aataaaatca atgtgttatt
    27241 taatcagttt attttatttt tccgaaacca cctatatttt tccgaaactc aaattttgac
    27301 cagcctattt tttagacctc caaattaccc tccaaaatat ttgggtgccc aaatggttgc
    27361 cgcagaaacc tagctgaatt ggttagagtt aagcaaacca acttatcatt gattttaagt
    27421 aagatgaccg aaacaaataa ataaaattta cgccctttaa atcatcatta aagggcgttt
    27481 aaattttaga atttataaac catattatcc tcatactgac cgctataact tgtaggtatt
    27541 aacaactatt cgctctgctc cctcaaatgc ttacttgctg tttagtttgg gttgctgaag
    27601 ccaaaaaagc aacagctaat gccactaaag
//
LOCUS       DANKBV010000016        27281 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.016, whole genome shotgun sequence.
ACCESSION   DANKBV010000016 DANKBV010000000
VERSION     DANKBV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27281
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.016"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(94..1029)
                     /gene="ftsX"
                     /locus_tag="PWY43_000553"
     CDS             complement(94..1029)
                     /gene="ftsX"
                     /locus_tag="PWY43_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDL2135460.1"
                     /translation="MIRSFSKLGAQTNYSLRAVWQDLMQRKLGTFLTVLVIAVSLTIP
                     TVSYLLWKNTHEAATQFYPEPQLTVYLHKNLAEHDVNTVVDRIRAFETDKIESFNYIS
                     RQQSLDEFRSWSGFGEALDILDENPLPAVVTLKPKANFNNADTMVELRNGLQQIKGVQ
                     EVRLDNGWLEKLTALTWLIARIAVVCTLLMLLAVFLVIGNSVRTDVANSKAAIEVQQI
                     LGATDHFIARPFLYTGMIYGLFGSLLAVLFSAVTISYFTGVVKYVTDMFTVQFELNGL
                     DFSEAFFLVVLCVFIGWLSAKIATMRNIFRIGSRG"
     gene            complement(1039..1692)
                     /gene="ftsE"
                     /locus_tag="PWY43_000554"
     CDS             complement(1039..1692)
                     /gene="ftsE"
                     /locus_tag="PWY43_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL2135461.1"
                     /translation="MIKFTNVNKVYKGGKPALQGIDFHLPAGGMAYITGHSGAGKSTL
                     LKLIMGIERANGGQILFNGHDITRLAHHELPFLRRQIGMVHQDYRLLTDRTILDNVAL
                     PLIILGMNQAIVEREARIALERVGLADKANYLPLHLSGGEQQRVDIARAIVHRPTLLL
                     ADEPTGNLDEKLSFEIFRLFEEFNQAGTTVLIATHDTNIISKRPKPCLVLEQGHLKF"
     gene            complement(1685..3181)
                     /gene="ftsY"
                     /locus_tag="PWY43_000555"
     CDS             complement(1685..3181)
                     /gene="ftsY"
                     /locus_tag="PWY43_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL2135462.1"
                     /translation="MSEKKKGFWSWLGFGKKEEAEQKEIQQEQESIQPEQPVIVEPET
                     EKSFSEKLDELEDKIEEKVDQLEDYFEEKEEKIEQFAEQVEDTIEQKVEDFKEFVEEK
                     VEEAKAFFDDVEDKIEQTFEEQKGEQAVENLQNIATEQLDLNEQEQVTYQVEEAVAEE
                     VAETELVNNVQDTVEESTIQDEYQEKPSQGGFFSRLIQGLIKTKQNIGSGFRNFFRGK
                     KIDDELFEELEEQLLVADLGMPTTQKIINNLTQHATKQQLKDADLLYQQLKVELGEVL
                     KPVAQPLVLEAKKPYVILMVGVNGVGKTTTIGKLARKFQQEGKSVMLAAGDTFRAAAV
                     EQLQVWGERNNIPVVAQSTGSDSASVIFDAMQSAAAKGIDVLIADTAGRLQNKNNLMD
                     ELKKIVRVMKKYDETAPHEIMLTLDAGTGQNAISQAKLFNEAVGLTGITLTKLDGTAK
                     GGVIFAIADQFNIPIRFIGVGEKIEDLRPFNAEEFIEALFSHEDEQND"
     gene            3260..3850
                     /gene="rsmD"
                     /locus_tag="PWY43_000556"
     CDS             3260..3850
                     /gene="rsmD"
                     /locus_tag="PWY43_000556"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL2135463.1"
                     /translation="MKKNRNSPQSVQQMGEVRVIAGLWRGRKLPVLNAEGLRPTTDRV
                     KETLFNWLMHDIAHSRCLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANQLKKNLQA
                     LKSEQGSVIHTDTLAYLAQKNNGEPFNIVFIDPPFHQGFVPQVLNLLAENNWLAPNAI
                     LYVETEKNHPPLELPQGWQILKEKAAGMVVSRLIQL"
     gene            3934..4245
                     /locus_tag="PWY43_000557"
     CDS             3934..4245
                     /locus_tag="PWY43_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDL2135464.1"
                     /translation="MKKLTPDEAIDIAYDIFLEMAGENLDPADILLFNLQFEERGAVE
                     MVETSEDWDQEIGTLIDPDAFAEVWVGLVNEQDEMDDVFARFLISHDEENREYHVVWK
                     E"
     gene            4267..4779
                     /gene="hpaC"
                     /locus_tag="PWY43_000558"
     CDS             4267..4779
                     /gene="hpaC"
                     /locus_tag="PWY43_000558"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HDL2135465.1"
                     /translation="MANIDTNQPIEASLKFREAMAHLSSAVSIVTTNGVAGKAGLTVS
                     SVCSVTDSPPTLLFCINKNSHVHDIIQQNGKVCVNVLSHEQEDLAKHFAAILESTMEE
                     RFGWDIWNNGFDDQPVLRSAISALQGNIVNTHAVGTHTIFVVEVSHIDVEPNHSLVYF
                     ARQFRSVNID"
     gene            4897..5769
                     /gene="tehB"
                     /locus_tag="PWY43_000559"
     CDS             4897..5769
                     /gene="tehB"
                     /locus_tag="PWY43_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HDL2135466.1"
                     /translation="MENLICYKQMPVWTKATLPQMFQEKHNTKVGTWAKLTILKGSLK
                     YYELTEEGEIIKEDIFDANHQRPMVEPQLWHKVEALSDDLECQLAFYCKPEDYFAKKY
                     NLTATHSEVLNAVNYTKGGKALDLGCGRGRNALYLNLLGFDVTAVDHNQESIDFLNYM
                     IEKEQAQNITTAVYDINEATIGSQNGEYDLIISTVVMMFLNRERIPAIIENMQKNTKV
                     GGYNLIVCAMSTDDYPCPMPFSFTFKEGELADYYQGWELVKYNEDLGHLHKTDENGNR
                     IQLRFATMLAKKIS"
     gene            5907..6944
                     /gene="purM"
                     /locus_tag="PWY43_000560"
     CDS             5907..6944
                     /gene="purM"
                     /locus_tag="PWY43_000560"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL2135467.1"
                     /translation="MSNTQLSYKDAGVDIHAGNELVERIKPDVKRTRRPEVMGGLGGF
                     GALCALPTKYKEPILVSGTDGVGTKLRLAIDLNKHDTIGQDLVAMCVNDLVVQGAEPL
                     FFLDYYATGKLEVDVATNVVKGIADGCVISGCALVGGETAEMPGMYHEGDYDLAGFCV
                     GVVEKSKIIDGSTVKTGDALIALASSGPHSNGYSLIRKVLEVSRSNPATDLLEDKPLS
                     EHLLAPTKIYVKSILKLVEEVDVHAIAHLTGGGFWENIPRVLPESAKAVIDESSWQWP
                     AIFSWLQEKGNISRYEMYRTFNCGVGMVIALPERDVEKALTLLKESGENAWLIGKIED
                     LGTGSEQVEIR"
     gene            7022..7300
                     /locus_tag="PWY43_000561"
     CDS             7022..7300
                     /locus_tag="PWY43_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL2135468.1"
                     /translation="MQLFWPIYFVLMNLAALYLMYSDKQKAIKKEWRVPEITLLSFCL
                     LGGAIGVYLGMKYFRHKTQHWYFHVAVVLSTFAWLIGLPYFYLALSSY"
     gene            7443..8870
                     /gene="aspA"
                     /locus_tag="PWY43_000562"
     CDS             7443..8870
                     /gene="aspA"
                     /locus_tag="PWY43_000562"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDL2135469.1"
                     /translation="MSDVRIEHDLLGEREVPNSAYWGIHTLRAIENFNISNVTISDVP
                     EFVRGMVMVKKATALANGELGAIPKKIAKAIVAACDEVLVKGRCLDQFPSDVYQGGAG
                     TSVNMNTNEVIANLALEILGEAKGKYDIVNPMDHVNASQSTNDAYPTGFRIAVYNSIT
                     KLINKITYLQTAFENKATEFSDVLKMGRTQLQDAVPMTLGQEFKAFAVLLDEEVRNLK
                     RTAELLLEVNLGATAIGTGLNTPEGYSTLAVKYLAEVTQLPCSLSENLIEATSDCGAY
                     VMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEV
                     VNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIELLSNACINLRDKCIDG
                     ITANRDICQNYVFSSIGIVTYLNPFIGHHNGDIVGKICATTGKSVREVVLERGLLTEE
                     QLDDILSIENLMNPTYKAKRFTEEE"
     gene            complement(8945..10963)
                     /gene="rep"
                     /locus_tag="PWY43_000563"
     CDS             complement(8945..10963)
                     /gene="rep"
                     /locus_tag="PWY43_000563"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDL2135470.1"
                     /translation="MKLNSQQQQAVEYTKGACLVLAGAGSGKTRVIINKIAHLIAHCG
                     YSPKQIAAVTFTNKAAREMRERVAHSIGKENSKGLTISTFHTLGFEILKREYKLLGFK
                     SGMTLFDEQDQIALLKHLLPENVTEDKDLLKALIATISNWKNDLLSPERVLTRVRDER
                     ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKSRWQQKIRYLLVDEYQD
                     TNTSQYELIKLIVGNEPNFTVVGDDDQSIYSWRGAKPENMQRLREDFDLQVIKLEQNY
                     RSSQRILHCANILIDNNDHLFSKRLFSELGQGEKLQVIEAKNEEHEAERIVGELIAHR
                     FSKKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLV
                     VNQDDDAAFLRIVNTPKREIGAATLEKLGILANEKQVSLFEAIFDFELIQRLTPKPYQ
                     ALQDFARWIVEIADQADRSDPIEAVKDLLAKIQYEAYLYEMAANPKVAEMQSRNVQTL
                     FEWVQAMLEGDEIEPPMNLVQVVNRLTLRDMLERGEEDDEADQVQLMTLHASKGLEFP
                     HVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKP
                     EPSRFLLELPQDDLEWESEKPRMTEQEKEAKSSANIANLMAMLKANKK"
     gene            11211..12326
                     /gene="potA"
                     /locus_tag="PWY43_000564"
     CDS             11211..12326
                     /gene="potA"
                     /locus_tag="PWY43_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDL2135471.1"
                     /translation="MEKVEQKAIIELKNVTKSYGSKTILKNLNLTINDGEFLTILGPS
                     GCGKTTALRLIAGFEDLTEGSIILDGQDVSNVPAEQRPVNTVFQSYALFPHMTIFENV
                     AFGLRMQKVPNAEIEPRVMEALRMVRLEDRAQQKPAQLSGGQQQRIAIARAVVNKPKV
                     LLLDESLSALDYKLRKEMQNELKALQRQLGITFIFVTHDQEEALTMSDRIIVMNAGKI
                     AQDGTPREIYEEPSNLFVARFIGEINVFDATVIERVDATNVKANVEGRICNIKVEDLE
                     VQPNQKLKVLLRPEDIVIEELDENESSKAIIGHIIDRNYKGMTLESSVRLDHNGMNVL
                     VSEFFNEDDPNIDHEVGQKVALTWYEGWEVVLNDEGE"
     gene            12313..13176
                     /gene="potB"
                     /locus_tag="PWY43_000565"
     CDS             12313..13176
                     /gene="potB"
                     /locus_tag="PWY43_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDL2135472.1"
                     /translation="MKANNKFQNGAVATIFGWLLLFVLVPNVLVLIISFLTENRQSQY
                     FVDFAFSFDAYKSLFNDTYAAVLWNSLYMAGIATFFCLIIGYPFAFFIAKMPERIRPF
                     LLFLVVLPFWTNSLIRIYGIKIFLGVKGFLNESLMALGIISEPMRLLNSEIAIIIGLV
                     YILLPFMILPLYSSIEKIDNRLLEAAKDLGANAFQRFIKVTIPLTMPGIVAGCLLVLL
                     PAMGMFYVADLLGGGKTPLVGNIIKSLFLNTNQFALGSAVSIALTILMALMLYVYYRA
                     NKLLNKKVELE"
     gene            13176..13943
                     /gene="potC"
                     /locus_tag="PWY43_000566"
     CDS             13176..13943
                     /gene="potC"
                     /locus_tag="PWY43_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDL2135473.1"
                     /translation="MKKMFRNLFMLVVFAYLYIPIIILVINSFNEDRFGLNWKGFTWN
                     WYERLFSNDTLVQATLNSLTIAFFAATFSTILGALTSIALYRYRFRGKKLVGGMLFVV
                     MMSPDIVMAVSMLVLFMILGIKLGFISLLIAHITFCLPYVVIAISSRLSDFDGKMLEA
                     AKDLGASEVTILRKIILPLALPSIISGWLLSFTISLDDVIVSSFVTSPSYEVLPLKIF
                     SLVKTGVTPEVNALATIMIIFSLTLVVLSQLVVKKKV"
     gene            14134..15057
                     /gene="oppB"
                     /locus_tag="PWY43_000567"
     CDS             14134..15057
                     /gene="oppB"
                     /locus_tag="PWY43_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDL2135474.1"
                     /translation="MLKFIFKRLLEAIPTLLILITFSFFLMRLAPGSPFTSEKAYPPK
                     VMANIEAKYHLNEPLYKQYFLYMENLSKGDFGPSFKYKDQSVNNLIASAFPVSIKLGI
                     TAFIFAVLIGLTAGTIAALKQNSKWDYLVMGFSMTGIIMPSFVFAPLLVLMFAIYLKW
                     LPAGGWNGGQLYYMVLPVLSLTISYVAGIARITRGSMIEVLHSNFIRTAKAKGLPMRK
                     IIFKHALRPALLPVITYLGPAFVGIITGSMVIESVFGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGTLTILFNAIVDILYAVIDPKIRYS"
     gene            15072..15971
                     /gene="oppC"
                     /locus_tag="PWY43_000568"
     CDS             15072..15971
                     /gene="oppC"
                     /locus_tag="PWY43_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDL2135475.1"
                     /translation="MQNSSKNQPLVEVTSTIQGRSLWQDARRRFFRNKAAVASLIILC
                     CIILFITFAPMLMPFSYEDTDWNMMGIAPDFESQHYFGTDSSGRDLLVRVAIGGRISL
                     MVGIAGALIAVLIGTIYGAISGYFGGKVDMVMMRFLEILSSFPFMFFVILLVTFFGQN
                     IFLIFIAIGMVAWLNLARIVRGQTLSLKNKEFVEAAIVCGVSRRQIIWRHIVPNVLGL
                     VVVYASLEVPSLILFESFLSFLGLGTQEPMSSWGALLSDGAAQMETSPWLLAFPAFFL
                     CLTLFCFNFIGDGLRDALDPKDK"
     gene            15981..17576
                     /locus_tag="PWY43_000569"
     CDS             15981..17576
                     /locus_tag="PWY43_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2135476.1"
                     /translation="MELLAVNNLDVYLKTDEELVHAVRNVSFSIEKGQTLAIVGESGS
                     GKSVTSMAIMQLLPKNIVSFGERSSIIFEEQNLLTLSEKAMQTVRGDRIGMIFQEPMT
                     SLNPYMPIGKQVAETVQTHNPQISNAEAEKMVLETLQKVKIPDAEKKMICYPHEFSGG
                     QLQRIMIAMAIINKPDLLIADEPTTALDVTTQAEILDLMHDLQRDMGMAIILISHDLR
                     LVHKYSDLVCVMQNGEIIERGETETVFTNPQHPYTVELLTPIPTNLKGELPPNAPTLL
                     QGEHIKVDYILKRSLFGKPKKVFNALKDISLHLKTGETLGIVGESGSGKSTLGRAIMQ
                     ILDYRGNITFAGKTLANLNKAERQALKKDMQMVFQDPFNSLSPRLTVGEIIAEGLTVH
                     CPQMSKTERREKVMKMLEEVNLNPAMINRYPHEFSGGQRQRIAIARAIILEPKFVLLD
                     EPTSALDRSTQITVIELLNRLQKKYGLSYIFISHDLAVIKALSDRVIVMSQGEIVESG
                     DVTQIFENPQQEYTKRLILASNL"
     gene            17756..18151
                     /locus_tag="PWY43_000570"
     CDS             17756..18151
                     /locus_tag="PWY43_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL2135477.1"
                     /translation="MKKLMIFATTAMIVSNLAHAAGEQSDAREAHESTVKTSTVKYSC
                     QNGKKLSVKYGFNKQGIPTYAEAKLSGKKRFMPINLYTTDATGTNFGDENNFSLYGDP
                     MEFTNHRKASVNIQSPASEILYKGCTPQK"
     gene            complement(18361..20520)
                     /locus_tag="PWY43_000571"
     CDS             complement(18361..20520)
                     /locus_tag="PWY43_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL2135478.1"
                     /translation="MQIKQTVLAIAITPFISQLSLAQETKISSIELDTIEVNEQQENL
                     LKQQAVMSNEDLVRYSTDMGVPQTGRFGPQGFNIRGVEGNQVAVTVDGISQAASNNYA
                     TFSRYSYYNKSRPEIDTELMSDVTIEKGSSTKVGNGALGGSVNYKTKTVSDILMKGRQ
                     FGGMLKYTYNGQNKENIETAGIAADGQYADALVMYSHRQGHETQTTSKGPDIMGSGRG
                     LPNPYKNNQYSYLAKLGLKWNDENRLELKTSKQRSKNAGQELSYSTVTLRDFLDVQEI
                     ETYGVTYLYTPKNSILENIKVAVTKQDTEISGINDQITIARGTKTDNTKRAFNTKNVE
                     GSLGVTFKPVDLGNTEHKLSTELGYGVSDFSFDMQTYTAGITPTDQNMQSPAKTKSFH
                     ILMGDSILLTQNLSAHLNARYEQSKLHSDDNSLSDKTFNNWAGDIGLNYQLSPVWQIG
                     YKLSRGFRTPTASEMFFNREVQGRGASQTFLANTNLKPETSLGNEISLVGNGSLGSLS
                     VAAYHTHYRNLIDLATIGNNIYQSQNVNRAKIKGVDINGTLDLNTAWNAIPQGFELNG
                     GFGYARGKRNDGTDLLTVQPLKALFGIGYRAVNNDWAIHLNGTYTQGKKAKYAQQYAI
                     LANTGYLSYDKSLTTYPYLSNSATVFDLVAHKKFGERAIVKVGVYNLFNKKYWTWDSL
                     RTIGITSPGVANSITGEGLNRYLAPERYVMASVEFKF"
     gene            20638..21504
                     /locus_tag="PWY43_000572"
     CDS             20638..21504
                     /locus_tag="PWY43_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2135479.1"
                     /translation="MEYKMRTLLFSFIALSMCLNTTKAAEKIVSTAGYASEIVAALGK
                     ADKLVGVDTTSVKPQTIMEKKPKIGYRRQLSAEGILSLTPDLIILAPDAGPNAVIEQI
                     KASGIPLLHLGEKPSLAGIREDIQQIAKAIGAESEAKQLTETILADEKKLADIRSQHS
                     TDKEVLVLIDTASQGIFGLGKKSVGDHFLTLLNLNNSFNAEGNKPLANEALAASNADV
                     ILLASRNQAKESATISKLAESSPQYAQLTNTQAGKKGCVFAINILDALGFGPYTAQYA
                     HQILTTIQPCLK"
     gene            21515..22501
                     /locus_tag="PWY43_000573"
     CDS             21515..22501
                     /locus_tag="PWY43_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2135480.1"
                     /translation="MKLHLTLIFTLIISLVVCTHLGAMSLPLSAWHCNNAECESAAYI
                     LWQIRLPRLLGTLFIGAALAISGATMQGLFRNPLVDPGIIGVSSGAALAAALYIVVIQ
                     PLLPESISYYALPIAAFLGGWLVTFMLYLLSQRNGQLHIAIMLLIGIALAAFSGALTG
                     ILIYIADDNQLRSLTFWSMGSLAGMNWQMVAMMAAVVFICSPLIWREHRALNALTLGE
                     EDAQYIGFDIAKVKRRLVFYVALLTGTSVACVGAIGFVGLVIPHLMRLLGGSNHRHLL
                     LNSLVCGAILLMIADTLARTLAAPAEIPIGIFTALFGAPFLAVMVWKSGKHI"
     gene            22501..23307
                     /locus_tag="PWY43_000574"
     CDS             22501..23307
                     /locus_tag="PWY43_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2135481.1"
                     /translation="MSNLLTCQNLSIVHQQKKLLEIQEIAFEQGKFTAIIGPNGAGKT
                     TLLKALSGNNAQTNSAVFYQHQRLTDLNGYALAQKRAMLSQQSQIAFPLKVEELVQLG
                     REPYRQSEFAKFNEKITAWAMETMSLTALKERSSIQLSGGEQHRAHIARVLAQLVPEP
                     NADLSGSWLLLDEPTNHLDIHHQYQLFSLLQKLKQQGLTIIAILHDPALAINQADHIL
                     MLKNGEKFAEMSAKAIAESCLLDELYEMKMGCRYCPHSGQYYLCPALAES"
     gene            complement(23328..23966)
                     /gene="purN"
                     /locus_tag="PWY43_000575"
     CDS             complement(23328..23966)
                     /gene="purN"
                     /locus_tag="PWY43_000575"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2135482.1"
                     /translation="MKKFVVLISGNGSNLQAMIDAQKSADTSGQICGVICNKADAYGL
                     IRAKQAGIPTFVFSRKDYQSNVEMDLAIAEQIEQLGAELIVLAGYMKILTPEFTQHFA
                     GKILNIHPSLLPKYPGLNTYQRAIEAGESEHGTTIHFVNEEVDAGAVVLQAKVPIYPE
                     DEIEDVMARVVEQEHRYYPLVIEWFCSGRLVSQHGKAYLDGKLLPACGYAED"
     gene            complement(24073..26271)
                     /locus_tag="PWY43_000576"
                     /pseudo
     CDS             complement(24073..26271)
                     /locus_tag="PWY43_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484758.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(26325..27161)
                     /locus_tag="PWY43_000577"
     CDS             complement(26325..27161)
                     /locus_tag="PWY43_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDL2135483.1"
                     /translation="MLPRISSFLASLFMAFFLLGCSVSTQSLNEQNVDSIGQIIDLNT
                     NQTISFTELLSRLSSQDYVLVGEEHTQLIHHQIELYLFNQLTKKAKLHLIALEMLNVD
                     QQPAIDRIQFNRPVNLSATDLREAIQWQKWDWKMYQDLVVESLGSNSRVIATNLTDKE
                     VEILLNGAEPLKGSVSTSSEVKQKIAQLLLSMNHGMPYPMENMVAVQQFRDRRMAEKL
                     VKNALSTSLLIAGNHHVRKDLGVPLHIAEYDRTKKVAVLMLKTEREEITSSQADYLWV
                     TQ"
BASE COUNT         7996 a         5657 c         5284 g         8344 t
ORIGIN      
        1 ctccacagca ataatatatt gcttatagta aaaaaccagt tcctaggaac tggtttttat
       61 ctgcaattat ttctctaaaa acaaccgctt gtattagcct ctactaccaa tacggaaaat
      121 atttcgcatt gtcgcaattt ttgccgacag ccagccaata aacacacaaa gcaccactaa
      181 gaagaaggct tcgctgaaat ctaatccgtt cagttcaaat tgtacggtaa acatatcagt
      241 cacatacttc actacaccgg tgaaatagct aatcgttacc gcactaaaca gcaccgctaa
      301 caggctgcca aacaagccgt aaatcatacc tgtgtataaa aacggacggg caataaagtg
      361 atcagttgcc cctaaaattt gttggacttc aatcgctgct ttgctattgg caacatcggt
      421 tcgcacactg tttccaatca ctaaaaaaac tgccaacagc attaataagg tacacaccac
      481 cgcaatacga gcaatcagcc aagttagagc agttaatttt tccagccagc cgttatctaa
      541 acgaacctcc tgcacccctt taatttgttg caaaccgttg cgtagctcca ccatcgtatc
      601 agcattatta aaatttgctt tcggttttaa ggtaacgacc gcaggcaacg ggttttcatc
      661 taaaatatct aacgcttcac caaagcctga ccaagaacga aattcgtcta aactttgttg
      721 gcgggaaata tagttaaatg actcaatttt atccgtctca aaagcacgaa tgcgatcaac
      781 aacggtattc acatcgtgtt ctgccaaatt tttgtgcaga taaacggtta attgtggctc
      841 agggtaaaat tgtgttgctg cctcgtgggt attcttccaa agtaaataac tcacggttgg
      901 aatcgtaagt gagaccgcaa tcaccagcac cgtcagaaaa gtgccgagct tgcgttgcat
      961 taaatcttgc caaaccgctc ttaaagagta gttggtttga gcacctaatt tactaaatga
     1021 acgaatcata atatttcctt aaaatttaag atgcccttgc tccagcacta aacacggctt
     1081 tggacgcttt gaaataatat tggtatcgtg ggtggcaatc agcaccgttg tgccggcttg
     1141 attaaattcc tcaaacaaac ggaaaatttc aaacgagagc ttttcatcta agtttccggt
     1201 tggctcatcg gctaataaca gcgttgggcg atgaacaatg gccctggcaa tatcgacacg
     1261 ttgttgctca ccgccggaaa ggtgcaacgg cagatagttc gctttatctg ccaagccaac
     1321 acgctcaagg gcaatgcggg cttcacgctc cacaatcgct tggttcattc ccaaaataat
     1381 caatggtaat gcgacattat ccaaaatggt tcggtcggtt aataagcgat aatcttgatg
     1441 caccatcccg atttggcggc gtaaaaacgg caattcatga tgagcaagtc gggtaatatc
     1501 gtgtccgtta aacaaaattt gcccgccatt ggcacgctca atccccataa ttaattttaa
     1561 tagcgtgctt ttccccgcac cggaatgccc ggtaatatat gccattccac cggcaggtag
     1621 gtggaaatca atgccctgta aggctggctt tccgccctta taaaccttgt tcacattagt
     1681 gaatttaatc attctgttcg tcctcgtggc taaataaggc ttcaataaat tcttctgcat
     1741 taaacgggcg tagatcttca attttctcgc ccaccccaat aaagcggatc ggaatattga
     1801 attgatcggc aatcgcaaaa atcacgccac cttttgccgt accgtctaat ttagttaagg
     1861 tgataccggt taaacccact gcctcattaa acaatttcgc ttggctgatc gcattttgcc
     1921 cagtgccggc atcaagcgta agcataattt cgtgcggtgc ggtttcatca tattttttca
     1981 tcacccgcac aattttcttc aactcgtcca tcaggttatt tttgttttgc aaacgccctg
     2041 cagtatcagc gattagcacg tcaatccctt ttgccgcagc ggattgcatc gcatcaaaaa
     2101 tcaccgaggc agaatccgaa ccggtgcttt gtgccaccac cggaatgtta tttcgctctc
     2161 cccaaacctg tagctgttca actgccgccg ctcggaacgt gtctcctgct gctaacatta
     2221 ccgatttgcc ttcttgttgg aatttacggg caagtttgcc aatggtagtg gttttcccca
     2281 cgccattcac accaaccatt aaaatgacat acggtttttt cgcttccaac actaacggtt
     2341 gagccaccgg ttttagcact tcacccagtt ccactttcaa ctgttgatac aacaaatctg
     2401 catcttttaa ttgttgcttg gtagcatgtt gtgttaaatt gttgatgatt ttttgtgtgg
     2461 tcggcatacc taaatccgcc acaagcaact gctcttccag ctcttcaaat aactcatcat
     2521 caattttctt gccacggaag aaattacgga agcccgaacc aatattctgc ttggttttaa
     2581 tcaagccttg aattaaacgg ctgaagaaac cgccttgact cggtttttct tgatattcat
     2641 cttggatagt agattcttca actgtgtctt gaacattatt tactaattca gtttcagcaa
     2701 cttcttcggc cacagcctct tcgacttgat aagtaacttg ctcttgttcg ttgagatcta
     2761 actgctcagt tgcaatattt tgaagatttt cgaccgcttg ttcgcctttt tgttcttcaa
     2821 atgtttgttc gattttatct tcaacatcat caaagaacgc tttcgcctct tcaacttttt
     2881 cttcaacaaa ctctttgaaa tcttcgactt tttgttcaat ggtatcttct acttgctcag
     2941 caaattgctc aattttttct tctttttcct caaaatagtc ttctaactga tctacctttt
     3001 cttcaatttt atcttccagc tcatctaatt tttcagaaaa cgacttctca gtttccggtt
     3061 caacaataac aggctgttcc ggttgaatac tttcttgttc ttgttgaatc tctttctgtt
     3121 cagcttcttc tttcttacca aatccaagcc aagaccaaaa cccttttttc ttctctgaca
     3181 tatttttctc tcatttctcg taaaaataga ctaaacttac atagtctatc aaaaaatccc
     3241 ttattttcct ttagtgagta tgaaaaaaaa tcgaaattcc ccccaatctg ttcagcaaat
     3301 gggcgaagta cgggttatcg ccggactgtg gcgaggcaga aaattacctg tactaaatgc
     3361 cgaggggctt cgcccgacaa ccgatagagt aaaagaaacg ctgtttaact ggttaatgca
     3421 cgacattgct cactcacgct gtcttgattg ctttgcaggc agtggctctc tgggcattga
     3481 agccctttcc cgccaagcac aagcggtcgt ttttttggaa aaatttgcaa atgctgccaa
     3541 ccagttaaag aaaaatctcc aagcattaaa atccgaacaa ggtagcgtta ttcacactga
     3601 caccctcgcc tatttagcac aaaaaaataa cggtgagcca tttaacatcg tatttatcga
     3661 tccgcctttt catcaagggt ttgtacctca agtgctaaac ttgctggcag aaaataactg
     3721 gctcgccccg aatgcgattt tgtatgtgga aaccgagaaa aatcacccgc cattagagct
     3781 accgcaaggc tggcaaattt taaaggaaaa agcagccgga atggtggtaa gccgtctgat
     3841 tcagctataa tctttttgtt ttttataact aaaaaatata aaataaaact tatctatcta
     3901 acattttatt aacactcaaa ttagaataac gaaatgaaaa aattaacccc tgatgaagcc
     3961 attgatattg cctacgatat tttcttagaa atggcaggcg aaaatttaga tcctgccgat
     4021 attttactgt tcaacctcca atttgaagag cgtggtgcag tggaaatggt cgaaacttct
     4081 gaagactggg atcaagaaat cgggacttta attgaccctg atgcctttgc cgaagtttgg
     4141 gttggcttag tcaatgaaca agatgaaatg gacgatgtat tcgcccgttt cttaatttcc
     4201 cacgatgaag aaaaccgtga atatcacgtt gtttggaaag aataagaaaa ataaaaagga
     4261 gaaaaaatgg ctaatatcga cactaatcaa ccaatagaag cctcgcttaa atttcgtgaa
     4321 gcaatggctc acttatcttc tgcggtgagc attgtgacaa ccaatggcgt agccggcaaa
     4381 gcagggctga cggtttcgtc tgtttgttcc gttaccgatt ccccaccaac gctcttattt
     4441 tgcattaata aaaacagcca cgttcacgat attatccaac aaaacggcaa agtatgcgtt
     4501 aatgtgttaa gccacgaaca agaagactta gcaaaacatt ttgccgccat tttagaaagt
     4561 acaatggaag aacgctttgg ttgggatatt tggaacaacg gctttgacga tcagcctgtg
     4621 ttacgcagtg ctatttctgc tttacaaggc aatattgtta atacgcacgc agtgggtacg
     4681 catactattt ttgtggtaga agtgagccat attgacgtag agccgaacca cagtttagtt
     4741 tattttgccc gccaatttag aagcgtgaac attgactaaa atatatttaa cagaaatttg
     4801 atcaaatccg acttttttag tcggattttt tatttcctgt ttttaaaaca gtgctaaaat
     4861 tttgccgttt atgtttgact gacaaaagga aacaaaatgg aaaatttaat ttgctacaaa
     4921 caaatgccgg tgtggacaaa agcaacctta ccacaaatgt tccaagaaaa gcacaacacc
     4981 aaagtcggaa cttgggcaaa actcaccatt ctcaaaggtt ctctcaaata ttatgaatta
     5041 accgaagagg gagaaattat caaggaagat atttttgatg caaaccacca acgcccaatg
     5101 gttgagcctc aactttggca taaggttgaa gctctttctg acgatttaga atgccagttg
     5161 gctttctatt gcaagccgga agattatttt gctaaaaaat ataatctgac cgctacccat
     5221 tcagaagtgc taaatgccgt caattatacc aaaggtggca aagcgttaga tttaggttgc
     5281 gggcgaggac gcaatgcact ttatctgaat ttactgggtt ttgatgtcac tgctgttgat
     5341 cataatcaag aaagcattga ttttctcaac tatatgatcg aaaaagaaca agctcaaaac
     5401 attactaccg ctgtttatga tattaacgag gcaactatcg gctcgcaaaa cggtgaatat
     5461 gacctgatta tttccactgt tgtaatgatg ttcttaaacc gtgaacgcat tcctgccatt
     5521 attgagaata tgcagaaaaa cactaaagtc ggcggttata acttaatcgt atgtgcaatg
     5581 agtaccgatg actacccttg cccgatgccg ttctccttta ccttcaaaga aggcgagctc
     5641 gccgattatt atcaaggttg ggaattagtc aaatataacg aagatttagg acatttacac
     5701 aaaactgatg aaaacggcaa ccgcattcaa ctacgttttg cgacaatgtt ggcgaaaaaa
     5761 atcagctaaa cttaaccgct tgcaggggga attagcttcc ccctaattca tcaaatccat
     5821 tctatttatt ttcacttttt cattccgcaa acgtttgctt gtgctataat ggcttcgttt
     5881 tttagtcatt tactgttgag gttaaagtga gcaacacaca actaagttac aaagatgccg
     5941 gcgttgatat tcacgcagga aacgagttag tcgaacgaat caagcctgat gttaaacgca
     6001 cccgtcgccc tgaagtaatg ggcggattag gtggttttgg tgctttatgt gctttaccga
     6061 caaaatataa agagccgatt ttagtttccg gtaccgatgg cgtcggcaca aaattacgct
     6121 tagccattga cttaaacaaa cacgatacta tcgggcaaga tttggtagcg atgtgtgtga
     6181 atgaccttgt cgtgcaaggt gctgaaccgc ttttcttctt agattattac gccaccggta
     6241 aattagaggt tgatgtagcc accaatgtcg tcaaaggcat tgcagacggt tgcgtgattt
     6301 caggttgtgc tttagtggga ggtgaaacgg ctgaaatgcc gggaatgtac cacgaaggcg
     6361 attacgattt agccggattc tgtgtcggtg tggtagaaaa atctaaaatc attgacggtt
     6421 caacggtgaa aaccggtgat gccttaattg cattagcgtc cagtggcccg cattccaacg
     6481 gctattcgtt aattcgtaaa gtattggaag tcagccgatc caatcctgca accgatttat
     6541 tggaagacaa acccttaagc gaacacttat tagccccaac taaaatttat gtaaaatcaa
     6601 tcctaaaatt agttgaagaa gttgatgttc acgctatcgc tcacttaacc ggtggcggtt
     6661 tctgggaaaa tattccacgc gtattgccgg aatctgccaa agcggtgatt gatgaatcga
     6721 gctggcaatg gccggcgata tttagctggt tacaggaaaa aggcaatatc agccgttatg
     6781 aaatgtaccg tactttcaac tgcggtgtcg gaatggtgat agccttacct gagagagatg
     6841 tcgaaaaagc ccttactctt cttaaagaga gtggtgaaaa tgcgtggctt atcggtaaaa
     6901 ttgaagactt aggcaccggt agcgagcaag ttgaaatccg ctaatttgca aaataaatgc
     6961 taaaattgac cgcttgtagt aactgatctg caagcggtta aatttttatt gaggaatgca
     7021 aatgcaacta ttttggccta tctactttgt cttaatgaac ctagctgcct tatatttaat
     7081 gtatagtgac aaacaaaaag cgatcaaaaa agaatggcga gtgccagaaa ttactctcct
     7141 ctctttttgc ctcttaggtg gggctatcgg tgtttaccta ggtatgaaat actttcgcca
     7201 taaaacccaa cattggtatt tccacgtcgc tgttgtgctt tctacttttg cttggctgat
     7261 tggcttgcct tacttttatt tagctttatc atcatattaa tttatcatta ctttttttaa
     7321 tccgaaagcc caatttatat cctagtccgc tcacaaaaaa tttgatttag atcgcattat
     7381 ttgtctatta aaatcgttat tatctaccaa aatcgattgt catttatttt aaggagatga
     7441 atatgtcaga tgtgcgtatt gaacacgatt tacttggtga aagagaagta cctaatagtg
     7501 cttattgggg cattcataca ttaagagcga ttgagaattt taatatctca aatgtcacca
     7561 tttctgatgt acctgaattt gtgcgtggca tggtaatggt aaaaaaagct actgcgttgg
     7621 caaatggtga attgggtgca atcccgaaaa aaattgccaa agctattgtg gcagcctgtg
     7681 atgaagtatt agtgaaagga cgctgtctcg atcaattccc ttcagatgtt tatcaaggtg
     7741 gagccggcac atcggtgaat atgaatacca atgaagtgat tgccaacctt gcattagaaa
     7801 ttttgggcga ggcaaaaggt aaatatgata ttgtcaatcc aatggatcac gttaatgcaa
     7861 gccaatccac caatgatgcc tatccaaccg gtttccgcat cgcggtttat aacagtatca
     7921 ctaaattaat caataaaatc acttacctac aaactgcatt tgaaaataaa gccactgagt
     7981 tttccgatgt actaaaaatg ggaagaacgc agttacaaga tgccgtgcca atgacgctcg
     8041 gtcaggaatt taaagctttt gccgtattat tagatgaaga ggttcgtaac cttaaacgga
     8101 cggcagaatt gcttcttgaa gtgaatttag gcgcaaccgc tatcggtact ggtctgaata
     8161 cgccagaggg ttactctact cttgcagtca aatatttagc cgaagtcacg caactgcctt
     8221 gctcactgtc tgaaaatctc attgaggcaa catctgactg tggtgcttat gtaatggttc
     8281 acggtgcatt aaaacgcacc gccgtcaaac tttctaaagt gtgtaatgac ttacgattac
     8341 tttcatcagg cccacgtgcc ggcttaaatg aaattaatct tccggaatta caagccggct
     8401 catctattat gccggcaaaa gtaaaccctg ttgtacctga agtcgtaaac caagtgtgct
     8461 ttaaagtgat tggtaacgac accaccgtta cttttgcagc agaggccggt caattacagc
     8521 ttaacgtaat ggagccggtt atcgggcaag caatgtttga atctatcgag ctgctttcca
     8581 atgcatgtat taatctacgt gataaatgta ttgacggtat taccgctaac cgtgacatct
     8641 gccaaaacta tgtgttcagc tctattggta ttgtgaccta cctcaatcca tttatcggcc
     8701 accataatgg tgatattgtc ggcaaaattt gtgcaaccac cggcaaaagt gtacgagaag
     8761 ttgtgcttga gcgaggctta ctcaccgaag aacaactcga tgatattctc tcgattgaga
     8821 acttaatgaa tccgacttat aaagccaaac gctttaccga agaggaataa tcattgttta
     8881 caagcggtct gattctggca aaaaattgca aatttttacc aaaatcagac cgcttgttat
     8941 cccattattt cttatttgct ttcagcatcg ccattaaatt tgcaatattt gcagaagatt
     9001 ttgcctcttt ttcctgttcg gtcattcttg gcttttcact ttcccactcc aaatcatctt
     9061 gtggcaattc cagtaaaaac cggctcggct caggtttgat gatttcgcca tattggcgac
     9121 gttctttaca cagtgaaaac gtgagcgttt gttgggctcg ggtaatgccg acataagcca
     9181 aacgccgctc ttcttctaca ttgtcctcat caaggctggt ttggtgtggc agaatcccct
     9241 cttccatacc gattaagaaa acgtgcggaa attccaaccc tttcgaggcg tgaagagtca
     9301 ttagttgaac ttgatcggct tcatcgtcct cttcgccacg ttcgagcata tctcgcaagg
     9361 ttaaacggtt taccacttgc accaaattca tcggaggctc tatttcatcg ccctctaaca
     9421 tcgcttgcac ccattcaaac aaggtctgca cattacggct ttgcatttcc gctacttttg
     9481 ggttcgccgc catttcatac aaataggctt catactgaat tttcgccaac agatctttta
     9541 ctgcttcaat cggatcggat ctatcggctt gatcggcaat ttccacaatc cagcgggcaa
     9601 aatcctgtaa ggcttggtaa ggctttggcg tgaggcgttg tatgagttca aaatcaaaaa
     9661 tcgcctcaaa caaactgacc tgcttttcat tagcaagtat gcctaatttt tctagcgttg
     9721 ccgccccaat ttcccgtttc ggcgtattca caatacgcaa gaaagctgca tcatcgtctt
     9781 gattcactac caaccgcaga tacgccatca tatctttgat ttccgctcga gcaaaaaatg
     9841 acgtaccacc cgaaatttta tacggaatgc ggttttgcat tagcaatttt tccaacaagc
     9901 gggattgatg gttgcctcga tacaaaattg cataatcttt gtatttggtc tttttcgaga
     9961 aacgatgggc aatcagctcg cccacaatgc gttccgcttc gtgttcctca ttttttgcct
    10021 caatgacctg taatttttcc ccttgcccca gttctgaaaa cagccgcttg ctaaacaagt
    10081 gatcgttatt atcaatcaga atattggcac aatgcagaat ccgctggctg gaacggtaat
    10141 tttgctcaag tttaatcact tgcaaatcaa aatcttcccg taaccgttgc atattttcag
    10201 gcttcgcccc acgccaagag taaattgatt ggtcatcatc gcccacgacc gtaaagtttg
    10261 gctcgttgcc gacaatcagc ttaattaact catattggct ggtgttggtg tcttgatatt
    10321 catcaactaa caaatagcga attttctgtt gccaacgaga ttttgcttcg ggaaattggc
    10381 ggaacagcaa caccggcaac ataatcaaat catcaaaatc caacgcatta taagccttaa
    10441 gttgattctg gtagagctga taaaaatgtg aaaacactcg ctcacgttca tcacgcacac
    10501 gagtaagtac cctttcaggc gaaagcaaat cgtttttcca gttagaaata gtggcaatta
    10561 aggctttcag cagatcctta tcttccgtca cattttcagg caataaatgt ttcagcaaag
    10621 caatttgatc ctgctcatca aacaaggtca tacccgattt aaaaccgagc aatttatatt
    10681 cgcgttttaa aatttcaaac ccaagtgtgt gaaaagtcga aatggttaaa cctttgctgt
    10741 tttctttgcc gatagaatga gccacccgtt cacgcatttc ccgagcagct ttgttggtaa
    10801 aggttactgc tgcaatctgc ttaggcgaat agccacaatg tgcaattaaa tgtgcgattt
    10861 tattaataat cacccgagtc ttacccgagc cggcaccagc cagcacaagg caagcccctt
    10921 tggtatattc tactgcttgt tgttgttgag aattgagttt cattgaatct attcaaaagt
    10981 aattttgaca actattttaa cggaaagaaa acaagcggtc gaattttcag gaaaatttgc
    11041 aaaaaacttt gaaaatccga ccgcttgttc atcagagaaa aatccccaaa acgcaaattt
    11101 ttcctttcaa tcttgcccct ttagctatat cattataagt tgattttttg ggggcaaacg
    11161 cctgatttta taactccttt tctttttaat ttaagggaaa aaaacagtag atggaaaaag
    11221 tagaacagaa agctatcatt gagcttaaaa atgtaacaaa aagctacggt agtaaaacga
    11281 ttcttaaaaa tttaaattta accattaacg atggtgaatt tttaacaatt ttaggccctt
    11341 ccggttgtgg taaaacaact gcgttacgtt taattgccgg ttttgaagat ttaactgaag
    11401 gttcaattat tcttgatggg caagatgttt ctaacgttcc ggcggagcaa cgcccggtaa
    11461 acaccgtatt ccaaagctat gcccttttcc cacatatgac gatttttgaa aacgtggctt
    11521 tcgggctgcg tatgcaaaaa gtgccaaatg ctgaaattga gccacgtgtg atggaagcat
    11581 tacgaatggt gcgtttagaa gaccgcgctc aacaaaaacc ggctcagctt tccggtggtc
    11641 agcaacaacg tattgcaatt gcccgtgcgg tggtaaataa accgaaagta ttgttgttag
    11701 acgaatcgct ttctgcgtta gattataaat tgcgtaaaga gatgcaaaat gaattaaaag
    11761 cattgcaacg tcaattaggc attaccttta tttttgtgac ccacgatcag gaagaagcct
    11821 taaccatgtc tgatcgtatt atcgtaatga acgctggtaa aatcgctcaa gacggtacac
    11881 cacgtgagat ttacgaagag ccaagtaact tatttgtagc tcgtttcatc ggcgaaatta
    11941 acgtttttga tgcaactgta attgagcgtg ttgatgcaac taatgtaaaa gcaaacgtag
    12001 aaggtcgcat ttgcaatatc aaagtggaag atttagaggt tcagccgaac caaaaattaa
    12061 aagtattgtt acgccctgaa gatattgtga ttgaggagtt agacgaaaac gaaagctcga
    12121 aagcaattat cggtcatatt atcgaccgta actacaaagg tatgacatta gagtctagcg
    12181 tgagattaga tcataacggt atgaatgtat tggttagcga gttcttcaat gaagacgatc
    12241 cgaacattga ccacgaagtg ggacaaaaag ttgctcttac ttggtatgaa ggctgggagg
    12301 ttgtgttaaa cgatgaaggc gaataacaaa ttccaaaatg gtgcggtcgc aactattttc
    12361 ggttggttat tacttttcgt attagtgccg aacgtactcg ttttaatcat cagcttctta
    12421 acagaaaacc gtcaaagtca gtattttgtg gactttgcat ttagttttga tgcttataaa
    12481 tcactgttta atgacaccta tgcagcagta ttgtggaact cgctatatat ggcgggcatt
    12541 gccacctttt tctgtttgat catcggctat ccctttgcat tttttattgc caaaatgccg
    12601 gaaagaattc gtccgttttt actgttttta gtagttctac cgttttggac taactcactg
    12661 attcgtattt atggcattaa gattttctta ggcgtaaaag gctttttaaa tgaaagcttg
    12721 atggctttag gtatcatcag cgagccaatg cgtttattaa actcagaaat tgcgattatt
    12781 attggtttag tttacattct attacctttt atgattctac cgctttattc atcaatcgag
    12841 aaaattgata accgcttact agaagctgca aaagacttag gtgcaaacgc cttccagcgt
    12901 tttattaaag tgactattcc attaacaatg ccgggcattg tcgcaggttg tttattagta
    12961 ttacttccgg caatgggtat gttctatgtg gcagatttat taggcggcgg taaaacaccg
    13021 ttagtaggta acattattaa gagcttgttc ttaaatacca accaatttgc attaggttca
    13081 gcagtaagta ttgcattaac tatcttaatg gcattaatgc tttatgttta ctatcgtgca
    13141 aacaaattat taaataaaaa ggtggagctt gaataatgaa aaagatgttt agaaaccttt
    13201 ttatgttggt tgtttttgca tatctctata ttccaattat cattttagtg atcaactcct
    13261 ttaatgaaga ccgctttgga ttaaattgga aaggctttac ttggaattgg tatgagcgtt
    13321 tattcagtaa tgatacgctt gtacaagcaa cattgaactc attaactatt gcgttctttg
    13381 cggcaacatt ctcaaccatt ttaggggcat taacctctat tgcgctttac cgttaccgtt
    13441 tccgtggtaa aaagctagtg ggcggaatgt tattcgtggt aatgatgtcg cctgatatcg
    13501 taatggcggt atctatgctc gtattattta tgatcttagg tatcaagcta ggctttattt
    13561 cgctattaat tgcacacatt actttctgtt tgccttatgt ggtaattgca atttcctcac
    13621 gcctcagcga ttttgacggc aaaatgctag aagctgcaaa agacctaggt gcaagtgaag
    13681 tgactatttt gcgtaaaatt attttaccac tggcattacc ttcgattatt tcaggctggt
    13741 tgttaagctt tacgatttcg ttagatgacg ttattgtttc atctttcgtg acaagcccaa
    13801 gctacgaagt tttaccactg aaaattttct cattggttaa aacaggtgta acacctgaag
    13861 tgaatgcact agcaactatt atgattattt tctcattaac attagtggta ttaagccagt
    13921 tagttgtgaa gaaaaaagtc taaaaatcgt atacataaat cggcacttta aagagtatta
    13981 aagtgccgat ttttttacaa aaataagcta aaaaaacaac cgcttaagaa taattaattc
    14041 ttataaaaat agccataact aatcaatatc tttacaaagc cgttaattta cgctattaat
    14101 tgcctaccaa tataactaaa aagagtggtg cttatgctga agtttatttt taaacgtctt
    14161 cttgaggcga ttccaaccct actaattctg attacctttt cctttttcct gatgcggctt
    14221 gccccgggta gcccatttac cagtgagaaa gcctatccgc cgaaagtaat ggcaaatatc
    14281 gaggcaaaat atcacctgaa tgaaccgctg tataagcagt attttcttta tatggaaaat
    14341 ctgtcaaaag gcgatttcgg tccatctttt aaatataaag atcaatcggt aaataatctg
    14401 attgcttctg cgtttcctgt ttcgataaaa ctgggcatta ctgcctttat ttttgcggtg
    14461 cttatcggtt taaccgcagg aactatcgct gcacttaagc aaaatagtaa atgggattat
    14521 ttagtgatgg gcttttcaat gacgggtatt attatgccga gctttgtctt tgctccgctg
    14581 ttggtactca tgtttgcgat ttatctcaaa tggttgcctg ccggtggttg gaacggtggt
    14641 cagctctact atatggtttt acctgttctc tctttaacca ttagctatgt tgccggcatt
    14701 gcccgtatta ctcgtggttc aatgattgag gtgttgcact ctaattttat tcgcaccgct
    14761 aaggcaaaag gcttgccaat gcgtaaaatt attttcaaac acgctttgcg tccggcattg
    14821 ttgcctgtga tcacttattt aggcccggct tttgttggca ttattaccgg ctcgatggta
    14881 attgaaagcg tgtttggctt accgggaatc gggcagttgt tcgtaaacgg agcattaaat
    14941 cgtgactatt cattagtatt aagcctaacc attttggtag gtacgctcac cattttattt
    15001 aatgcgattg ttgatatttt atatgcggtc atcgacccga aaattcgtta ttcataggag
    15061 taaagcggaa aatgcaaaat tcttcaaaaa atcaaccgct tgttgaggtt acctcaacca
    15121 ttcaaggacg aagtttatgg caagatgcac gccgccgttt tttccgaaat aaggcggctg
    15181 tggcaagttt gattattctt tgttgtatta ttctttttat tacctttgcc ccaatgctaa
    15241 tgccgtttag ctatgaagat accgattgga atatgatggg gattgctccg gattttgaat
    15301 ctcaacacta tttcggtact gattcttcag ggcgtgattt gctggttcga gttgcaatcg
    15361 gtggacgcat ttcgttaatg gtagggattg ccggtgcatt aattgcggtg cttatcggta
    15421 caatctatgg agcaatttcc ggttatttcg gcggtaaagt cgatatggtg atgatgcgtt
    15481 ttctggaaat tttaagctca ttcccattta tgttcttcgt aattttgctg gttacgttct
    15541 tcgggcagaa tattttcctg attttcattg caatcggtat ggtagcgtgg ctgaaccttg
    15601 cccgtattgt acgtggtcaa actctttcgc ttaaaaataa agagtttgtg gaagcagcga
    15661 ttgtgtgtgg cgtttcccgc cgtcaaatta tttggcgaca cattgtgcct aacgttttag
    15721 gcttagtggt ggtttatgcc tcccttgaag taccatctct gattttgttt gaatcgttct
    15781 taagtttctt agggcttggt acacaagagc cgatgagcag ttggggggca ttattaagcg
    15841 acggagcggc acaaatggaa acctcgcctt ggctactggc tttccctgcc tttttcttat
    15901 gtttaacact gttttgtttt aattttattg gtgatggctt gcgtgatgcc ctcgatccaa
    15961 aagataaata gaggtttaga atggaattat tagcagtaaa taatctcgat gtttatttaa
    16021 aaacggacga agaattagtt cacgcagtgc gtaatgtctc gttctcgatt gaaaaagggc
    16081 aaacccttgc gattgtgggg gaatcgggtt cgggtaaatc cgttacttca atggcaatta
    16141 tgcagttatt gcctaaaaat atcgtctcat tcggtgaacg ctcaagcatc atttttgaag
    16201 aacaaaactt gcttactcta tctgaaaaag caatgcaaac agttcgaggt gatcgcatcg
    16261 gtatgatttt ccaagagcca atgacctcac tcaatcctta tatgccgatt ggcaagcagg
    16321 ttgctgagac ggttcaaacc cataatccgc aaatcagcaa tgccgaagcg gaaaaaatgg
    16381 tgttggaaac tttgcaaaaa gtaaaaattc ccgatgcgga aaagaaaatg atttgctatc
    16441 cgcacgagtt ttccggcggt cagctacaac gaattatgat tgcaatggca attatcaaca
    16501 aacccgattt attgattgcc gatgagccga ccaccgccct ggatgtcaca acccaagcgg
    16561 aaattttgga tttaatgcac gacttacagc gtgatatggg aatggcgatt atcttaattt
    16621 cccacgattt acgcttggtg cataaataca gtgacttagt ctgcgtaatg caaaacggcg
    16681 aaattattga gcgaggcgaa acggaaactg tgttcaccaa tccgcaacat ccttatacgg
    16741 tggagctact taccccgatt ccaactaatt taaaagggga attaccgcca aatgcaccaa
    16801 ccttgcttca gggtgagcat attaaggtag attatatttt aaaacgctcg ctattcggta
    16861 agccgaaaaa ggtgtttaat gcgttaaaag atatttcatt gcacttgaaa accggcgaaa
    16921 cgctcggcat tgtaggcgaa tccggctccg ggaaatctac ccttgggcgt gccattatgc
    16981 agattttaga ttatcgcggc aatattacat ttgcaggtaa gacactggct aatctgaata
    17041 aagccgaacg tcaagccctg aaaaaagata tgcaaatggt gttccaagac ccgttcaact
    17101 cactttctcc tcgcttaaca gttggggaaa ttattgctga gggcttaacc gtacattgcc
    17161 ctcaaatgag caaaaccgaa cggcgggaaa aagtaatgaa aatgttggaa gaggttaatc
    17221 tcaaccctgc aatgattaac cgctatccgc acgaattttc gggcggacaa cgccagcgga
    17281 ttgcgattgc aagggcaatc attttagagc cgaaatttgt attgcttgat gagccaacct
    17341 cagcacttga ccgctctact cagatcactg taattgaatt gcttaaccgc ttacagaaaa
    17401 aatacggctt aagttatatt ttcatcagcc acgatttggc ggtcatcaag gcacttagcg
    17461 accgagtgat tgtaatgagc caaggtgaga ttgtagaaag cggtgatgtc actcaaattt
    17521 ttgaaaatcc acaacaagaa tacaccaagc ggttgatttt agcctctaat ttgtaacccc
    17581 ttccacaagg tcttattcta ccaaaaaaca agaccttgtc attttatgct agcaaaacat
    17641 tgtaattttg atattttatt tcaatttttt gaaaaaatat ggaactttat ataaaactca
    17701 ctttctaaac aatacgttat ctgaactaag ataacgctta tttaaaggag aatatatgaa
    17761 aaaattgatg atattcgcaa caactgccat gattgtaagc aatttagccc atgcagcagg
    17821 tgaacaaagt gatgcaagag aagcccacga aagcaccgta aaaacctcaa ctgttaagta
    17881 tagctgccaa aacggcaaaa aattaagcgt gaaatacggc ttcaataaac aaggcattcc
    17941 aacctatgct gaggcaaaat taagcggtaa aaaacgtttt atgccgatca acctatacac
    18001 gacagatgca accggcacta actttggtga cgaaaacaat ttcagccttt acggtgatcc
    18061 aatggaattt accaaccacc gtaaagcatc agtaaatatc caaagccccg caagtgaaat
    18121 tttatataaa ggttgcacac ctcaaaaata gataaaaagt aaaatgagtc cgctctgata
    18181 agtggctttt taatagctgt ttattgcaga taggaaagca ttttgttttt atctactatc
    18241 cagaaacaag cggttaaatt tgtttaattt tttacaaaaa atgggccaaa attaaccgct
    18301 tgtttattac tataaagaaa taagtacgta aaaattaact cttacattta atgcttatct
    18361 ctaaaattta aactccactg atgccattac atagcgctct ggagcaaggt aacgatttaa
    18421 gccttcgcca gttatactgt tggcaactcc aggactagtt ataccaatag ttcttaaact
    18481 atcccaagtc caatattttt tattaaagag attataaacc ccaactttaa caatcgctct
    18541 ttctccaaac tttttgtgtg caactaagtc aaatacggtg gctgaattac ttaagtatgg
    18601 gtaagtggtt aatgatttat cgtaacttaa atacccagta ttggctaaaa tagcatactg
    18661 ttgtgcatat tttgcttttt tgccttgtgt gtatgtgccg tttaggtgta tcgcccagtc
    18721 attgttcaca gctcgataac ctatgccgaa caatgctttt aacggttgaa ccgtgagtaa
    18781 gtcggtacca tcattacgtt tgccacgagc ataaccaaat ccaccattaa gctcaaagcc
    18841 ttgtggaatt gcattccacg ctgtgtttaa gtctaaagtt ccgttaatat caacaccttt
    18901 gattttagct cggttaacat tttgcgattg gtaaatattg ttaccaatag tggcaagatc
    18961 aatcagattt ctatagtggg tatggtaagc ggctacgctc aagctaccta atgaaccatt
    19021 acctaccagt gaaatttcat tacctaagct tgtttccggt tttaagttag tatttgctaa
    19081 gaaagtttgg cttgctccac gcccttgaac ttcacggtta aagaacattt cacttgcggt
    19141 aggtgttcta aaaccacggc tgagtttata gccaatttgc catacaggag agagctgata
    19201 atttaaacca atatctcccg cccaattgtt aaaggtttta tctgacaaac tattatcatc
    19261 agaatgtaat ttcgactgct catatcgagc atttaaatga gcagatagat tttgtgtcag
    19321 taaaatagag tctcccatca aaatatggaa agacttggtt tttgccggag actgcatatt
    19381 ttgatcggtt ggcgtaatac ctgctgtata ggtttgcata tcaaaactga aatcagatac
    19441 accatagcct aattccgttg acaatttatg ttcggtatta cctagatcaa caggcttgaa
    19501 tgtaacacct aagctacctt caacattttt agtattaaaa gcccgtttgg tattatctgt
    19561 ttttgtacca cgggcaatag tgatttgatc gttaatacct gaaatttcag tatcctgttt
    19621 ggttaccgca actttaatat tttcaagaat actgtttttt ggagtgtata aataagtaac
    19681 gccataagtt tcaatttcct gcacatccaa aaaatcacgt aatgtgacgg tagaataaga
    19741 aagctcttga ccggcgtttt tactgcgttg cttagaagtt tttaattcaa ggcgattttc
    19801 atcattccat tttagcccta attttgccaa gtagctgtat tgattatttt tgtaaggatt
    19861 tggtaaacca cgaccacttc ccataatatc cggaccttta gacgttgttt gtgtttcgtg
    19921 tccttgacgg tggctataca taaccaaagc atccgcatat tgaccatccg cagcaatacc
    19981 tgccgtctca atattctctt tattttgacc gttataggta tatttcaaca tcccaccaaa
    20041 ttggcgacct ttcattaaaa tatcacttac ggttttagtt ttatagttca cagagccgcc
    20101 taatgcccca ttgcctactt ttgtgcttga gcctttttca atggttacat cgctcattaa
    20161 ttcagtatcg atttcagggc gagatttatt gtagtaacta tagcgactaa aggtggcata
    20221 gttatttgat gctgcttgcg atatgccatc aactgttact gccacttgat taccttcaac
    20281 accacggata ttaaaccctt gtgggccaaa gcggcctgtt tgcggaacgc ccatatcagt
    20341 actataacgt actaaatctt cattgctcat caccgcttgc tgtttaagta ggttctcctg
    20401 ctgctcattt acttcaatag tatctagttc aatgctacta atctttgttt cttgagctaa
    20461 ggagagttga gaaataaagg gagtaatcgc tattgctaat acagtttgct ttatttgcat
    20521 attttagatc ctctagttag tttaatccgt ttaatgttaa aaaaatgtta acactatatc
    20581 attttaattg aaaattgaaa taataattat tattatttaa gtttagataa aaatcaaatg
    20641 gagtataaaa tgagaacctt acttttttct ttcattgccc tatcaatgtg tttaaatacc
    20701 acaaaggcgg cagaaaaaat tgtttcaact gcgggctatg cttccgaaat tgtcgcagca
    20761 ctaggaaaag ctgataagtt agtcggtgtg gataccactt cagttaaacc tcaaacgata
    20821 atggagaaaa aacctaaaat cggttatcgt cgccagcttt ctgctgaagg tattctctct
    20881 ttaaccccag atttgattat tttagcccct gatgccggcc ctaacgcagt tattgagcaa
    20941 attaaagcca gcggtattcc attacttcac ttaggagaga aaccgagctt agcagggatt
    21001 cgtgaggata ttcagcaaat tgccaaggcg attggtgcag aatcagaggc aaaacaatta
    21061 acagaaacca ttctggcaga tgaaaaaaaa ttagccgaca ttcgtagcca acattctact
    21121 gacaaagaag tcttagtgct gattgacact gcatcgcaag gtatctttgg gctagggaaa
    21181 aagagtgtgg gtgatcattt cttaaccttg ttaaatctaa acaatagttt caatgctgag
    21241 ggcaataagc cactcgccaa tgaggcttta gctgcaagca atgccgatgt tattttatta
    21301 gcctcccgta atcaggcaaa agaatctgca acgatcagta aattagcaga atccagccca
    21361 caatacgccc aactcacaaa tacacaagcg ggcaaaaaag gctgtgtttt tgcaatcaat
    21421 attttagatg ccttgggatt tgggccttac actgctcaat atgcacacca aattctcacc
    21481 actattcaac cttgtttaaa ataacggata cgctttgaaa ctgcatttaa ccttaatttt
    21541 cactttaatt atcagcttag tggtttgtac ccatttaggg gcgatgtcat tacccctatc
    21601 cgcttggcac tgcaacaacg ctgaatgcga aagtgcagcc tatattttat ggcaaatccg
    21661 cctgcctcga ttactaggga cattattcat cggggcagct ctcgctatta gcggagcaac
    21721 aatgcaaggg ctgtttcgca atccgttagt agatccgggg attattggtg tctcttcagg
    21781 tgctgcattg gctgcggcat tatatatcgt ggtgattcag ccactactgc cggaatcaat
    21841 cagctattat gctctaccta ttgccgcctt tttagggggc tggctggtga cttttatgct
    21901 ctatttactt tctcaacgta acgggcaact acatatcgcc attatgctat taattggtat
    21961 tgctctagct gcatttagtg gcgctttaac cggcatactg atttatattg ccgatgataa
    22021 ccaacttcgt agtttaacct tttggagtat gggatcttta gcaggtatga attggcaaat
    22081 ggtggcaatg atggctgcgg ttgtcttcat ttgtagccca cttatttggc gggaacaccg
    22141 tgcgttaaac gccttaacat taggtgaaga agatgcacaa tacattggtt ttgacattgc
    22201 aaaagtcaaa cgccgtcttg tgttttacgt tgccttatta accggcacaa gcgttgcctg
    22261 tgttggggct atcggctttg tcgggctggt tattccacat ttaatgcgtt tactgggagg
    22321 cagtaaccac cgccatttat tactcaatag tttagtttgc ggtgcaatct tattaatgat
    22381 tgcagatact cttgccagaa ccctagccgc cccggcagaa atcccgattg gtatttttac
    22441 cgccttattt ggtgcgccat tccttgcagt aatggtatgg aaaagtggga aacatatcta
    22501 atgagcaatt tattaacttg ccaaaattta agcatcgttc atcaacaaaa aaagttgctg
    22561 gagattcaag aaattgcctt tgaacaagga aaatttaccg ccatcatcgg tccaaacgga
    22621 gccggcaaaa caaccttgct caaagcctta agcggtaata atgcacaaac taacagtgcc
    22681 gtattttatc aacatcaacg cttaacagac ttaaacggat atgctctggc tcaaaaacgg
    22741 gcaatgctca gccaacagag ccaaattgcc tttcccttaa aagtagaaga attggtacaa
    22801 ctcggaagag agccatatcg ccaaagtgaa tttgcaaaat ttaatgaaaa aataaccgct
    22861 tgggctatgg aaacaatgtc tttaaccgca ttaaaagaaa gaagtagcat acaactttcc
    22921 ggtggagaac aacatcgagc gcatattgct cgagtgctgg ctcagctcgt tcctgaaccg
    22981 aatgccgatt taagcggaag ctggcttttg cttgatgaac ccaccaacca tttggatatt
    23041 catcaccaat atcagctttt ttccttacta caaaagctga aacaacaagg cttaactatc
    23101 attgcgattt tgcacgatcc tgcattggca ataaatcaag ccgatcatat cttaatgcta
    23161 aaaaatggag aaaaatttgc tgaaatgtcc gctaaagcca ttgcagaaag ttgtttatta
    23221 gatgagctgt atgaaatgaa aatgggctgt cgctattgcc ctcacagcgg gcaatattat
    23281 ctttgccctg cattagctga aagttagggt gcgatgcacc ctatcgttta atcttctgca
    23341 tatccacaag caggcagtag tttaccgtct aaatacgctt tgccgtgctg agaaactaat
    23401 ctacccgaac agaaccactc aatcaccaac ggataatatc gatgttcttg ctcaaccact
    23461 cgagccatta catcttcaat ctcatcttca ggataaatcg gcactttagc ttgtaatacc
    23521 accgcaccag catccacttc ttcattcacg aaatgaatag tcgtgccgtg ttcactttcc
    23581 ccagcctcta tcgctctttg ataagtgttt agccctgggt atttaggtaa taaggaaggg
    23641 tgaatattga gtattttgcc agcaaaatgt tgcgtaaatt caggcgttaa aattttcata
    23701 tagccggcta atacaattaa ctcagctcct aactgctcaa tttgttcggc aatcgcaaga
    23761 tccatttcca cattgctttg gtaatcttta cgagaaaata caaatgtcgg gattcccgcc
    23821 tgtttagctc gaatcagccc ataggcatct gccttattgc aaattacacc gcaaatttga
    23881 ccgcttgtat ccgcagattt ttgcgcatca atcattgctt gtaggttaga accattccct
    23941 gaaattaaaa ccacaaattt tttcatatta cacaccggct gaatattcat cgttctggat
    24001 attatgcctt gaatctcaac atcagtacag gaaagaaaaa gggcttattg gttaaaataa
    24061 gccactttca agctagaaac gcacctctgc ccctagtgtg taagttcgac ctctggcact
    24121 atttgtgaaa cgataggtgt ctgtatcata gtcatactct tctgcggtgg aagagttata
    24181 cgcatttaag gcatcaagat aatttcggtt catcacattt tgtaccccgg cttttaaggt
    24241 taaatgttta ttcactttgt aatttgcata taaatcgata atcaccggaa tattcggtaa
    24301 ctgctcgtga ataatgttgc catcttcgtc taaatcaata ccgacacgtt tcgctttgcc
    24361 tgtatattta aaaatgctag acagtgttaa ttttttatcc agcaatttag cccctagctc
    24421 caaagtcgca taatcttttg gtagctgggt ataagcttca gtgccaaatc cataaagaat
    24481 agtcgcacct tttgaagtgg gtgctgtgct ttcagttcgg gtataactac cattgacaaa
    24541 aaagctatcg gaatcgtaag ccagctcaac ttcccaacct cgatttttaa ccttttcagg
    24601 gtgattcaca taaatttgga actgtatggc attggtagca tcacttgaat ttcgacatac
    24661 tgttggccca caaccataca acgagaggtt atacaaatag tctttaatgc gtgagttaaa
    24721 atataccgct tttagcccca agatatcatt attgagtaat acattatctt gatgaatatt
    24781 aaacccaatc tcataggttt tcgcactttc tggtttcaaa aacgggttca cactcaagcc
    24841 atttcgactg gtattaaaca tttcttgcac attaggcgaa cgggaggttt gtttgtaact
    24901 gacaaaaggt tgaaaccaat cagcaacctg aaatgctaaa gtggcagaag gattcaactt
    24961 ataatgtttt ttattaacat caaacccaaa agaagggaaa caataatcca tatcatcgct
    25021 atcgcattct ggcttatatc cttttaactg atagcgtgaa tagtgaagac ttgaatttaa
    25081 agtgacaatg ccgtatttcc attcggtgtc taggtaaagg gaatcaatat tttgcttacc
    25141 tgccggccca aaagcggcat tgctaatcgc tccatcatta ttcttacttt ccggtaaatc
    25201 acgggaatat ttatttcgca ttaaatttat accataagtc aataatgctg ataatttccc
    25261 tgactcaaaa cggctggtgt tattcaaatc aaacatccac attttgtttt tggttgaggc
    25321 atcttctaat gtccatagtt ttgcatcatc gtggaaatct tgcttagttt ctgttactgc
    25381 tgttaggaat ttcagatcaa tccaatcaga ttgaggctgg taactggatt ctaaggaata
    25441 ggtcacacta tcaatattac gccccactaa gctattttta tattgacgtc cggacaattt
    25501 aaactctgaa tcttgatttg gcttaaaggc catttttccc atataagatt cagggtgatt
    25561 ttttaaccct tgcatataag cagaatccgt tgccattgtg ccattacctc ggcgataatt
    25621 ttgactgata ctacttttgc taaaagccaa taaactgcca agagaaccac tttccagcac
    25681 atcaactttt ccggctagtg caagcattcc gttttttcac agatcattat tgccataatt
    25741 aaaacggctt aatacgccga ctttatgttg ttctagtaga atatcttcga catcaatcgt
    25801 tttaaaatta gcactaccgg ctaaagcatt caatccggct gcaccattgt tgtagccttt
    25861 cgcaatatca actcgggtta aaaaattttg atcaattaaa gtgctgaatt ggctcattgg
    25921 cccgttacca tcagtgtgga acttaccacc gccgtttgct gatgtaccaa gctgtgtttg
    25981 tggcacgcca tcaactaagg tatttacccg accaagacca ctcatattac gaatattcac
    26041 actcacgccc ccttgccccg ggtcaatttg ggtatagcca cctgcgacag aacggacaat
    26101 actatctaaa ctttgcattg cattcacatt cattgctcga gaactcaccg agccggcttt
    26161 actgaaattc tcaaaattag ggttttccac agtgccataa accgtcacag tatcaagctc
    26221 cgcaatattt ttcgcctttt cctctgccaa tgcagtttgc gatcctccca tacacaaaac
    26281 cggaaacaaa atgcgtgtga ttatattaaa tttcataatt ctccttattg agtgacccaa
    26341 agataatctg cctgtgaaga agtaatttct tctctctctg ttttaagcat tagcaccgct
    26401 actttttttg ttcggtcata ttccgcaata tgcaacggaa cgccaagatc tttgcgaacg
    26461 tggtggttac ctgcaatcag caagctagtc gataacgcat tttttactaa tttttctgcc
    26521 attctgcgat cacggaattg ttgtactgcc accatatttt ccatcgggta aggcattccg
    26581 tgattcattg acagcaacag ttgtgcgatc ttttgcttaa cttcagagga agtggataca
    26641 gaacctttta atggctccgc cccatttaat aaaatttcaa cctctttatc tgttaagtta
    26701 gtggctataa cacgactatt tgaacctaag ctctctacca ctaaatcttg atacatcttc
    26761 caatcccatt tttgccattg aattgcctct cttaaatccg tggctgataa attaaccggt
    26821 cggttaaact gaatgcgatc aatcgcaggc tgctgatcca catttaacat ctccaacgca
    26881 attaaatgta acttggcttt cttcgtaagt tgattaaata aatacaattc aatttggtgg
    26941 tgaattaact gagtatgctc ctcaccaact aaaacgtaat cttgagaaga tagacgggaa
    27001 agtagttcag taaatgaaat cgtttgattg gtattcagat caataatttg gccgatagag
    27061 tccacgtttt gttcattaag ggattgcgtt gaaaccgagc agcctaaaag aaaaaaagcc
    27121 ataaaaaggc tagctaaaaa agaggaaata cgaggtaaca taacaaaaaa taaattaatg
    27181 agaattatta tcattatgtt atgtaaaaaa agaaaaatca atccattttc agaaaacaga
    27241 taaagaaaat gattgataaa acaaaaagcg gtaatatttc t
//
LOCUS       DANKBV010000017        26965 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.017, whole genome shotgun sequence.
ACCESSION   DANKBV010000017 DANKBV010000000
VERSION     DANKBV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 26965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26965
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.017"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1242
                     /locus_tag="PWY43_000578"
     CDS             <1..1242
                     /locus_tag="PWY43_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2135484.1"
                     /translation="QVGAGNPISAEEVAAADLVFVAADIDVDLEKFKGKPMYRTSTGL
                     ALKKTTQEFEKAFAQAKIYEGGVAKADNAEVPTSEKKGLYKHLMTGVSHMLPLVVAGG
                     LLIAISFMFGIEAFKDETIAGGLPKALMDIGGGAAFHLMIAVFAGYVAFSIADRPGLA
                     VGLIGGMLATTAGAGILGGIVAGFLAGYTVKFLNGAIQLPASLTSLKPILILPLLGSA
                     IVGLLMIYVINPPVKALMDALVEWLNTMGQTNAIILGIILGAMMCTDMGGPVNKAAYT
                     FGVGLIASQQYMPMAAVMAAGMVPPIGMAIATLIARSKFNTNQRDAGKASFVLGLCFI
                     SEGALPFVAADPVRVIASSILGGATAGAISMALGITLQAPHGGLFVIPFVSQPLMYLA
                     AIAIGSLVTGVVYAAIKQKAE"
     gene            1409..1927
                     /locus_tag="PWY43_000579"
     CDS             1409..1927
                     /locus_tag="PWY43_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HDL2135485.1"
                     /translation="MILLRGDTMQFIEFTDPDSGISYQYSEFTIANVAFIINFCSDAD
                     VISTLSALGKDITNYINTYSCCTIKFMAKEHLENSGSNIDIYAPAANHQFKRKEIIAL
                     QETLERLLFEHYVRFTPESYLFIAERDSLNRMYQHMCVPRCDFMQSFQVVYPLGVNQD
                     CFILITPKGNLK"
     gene            complement(2080..2943)
                     /gene="rarD"
                     /locus_tag="PWY43_000580"
     CDS             complement(2080..2943)
                     /gene="rarD"
                     /locus_tag="PWY43_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL2135486.1"
                     /translation="MLRGILASLAANVLFGAVYYFAILLRPLEGEAMFGFRIVVVIPF
                     ILLAIILFKQQSAFADLLQKIRQNPPLVLVILLLALNTGVQLWLFLWAPNHGQALQVS
                     FGYLLLPIVAVALGKMVFKEHFTRLKWLSLLFAVIGVGSSLFFNSTISWATWLAGLGY
                     PLYITLRRYFQINTLATFFVELLLVLPVALYYLSQVEMTPIVAENPQIYFYLGLLGLV
                     SGSAFILYILSSNLLPINVLGLLGYVEPLVMLLISFAIGEKLDANSYILMVCLLVSIG
                     LLSWDSFRSKV"
     gene            3116..3700
                     /locus_tag="PWY43_000581"
     CDS             3116..3700
                     /locus_tag="PWY43_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDL2135487.1"
                     /translation="MKKIFWILLIAVLAVGGYSLTKYNDLMRAEEDINSVWGNVESAY
                     QRRADLIPNLVNTVKGQANFEKETLTSVIEARAKATAVTIDPSNATEEQMAKFQEAQQ
                     GVNSALSRLLVSVEKYPELKAHEAFLNLQAQLEGTENRINVERNNFNEKVKEYNKQVR
                     EFPTKLAAMIMGFKAKPQFKSEAGSDKAPTVNFN"
     gene            3782..4240
                     /locus_tag="PWY43_000582"
     CDS             3782..4240
                     /locus_tag="PWY43_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2135488.1"
                     /translation="MGLFSFLSNKLPIDTQRIEQAIADLEQKTSAELRVVVERKAKIG
                     KTDNAAMLRASELFDELKMRETAEHNGVLIYLSFKPHYAAIIGDEGIHQKVDNDFWQK
                     IYATMCLDCQQGDYTQAICDAIKMVEAPLAHHFPYQADDKNELSNEVVVK"
     gene            4237..5073
                     /locus_tag="PWY43_000583"
     CDS             4237..5073
                     /locus_tag="PWY43_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2135489.1"
                     /translation="MMKSLCKFPSIFNRLLLVCLALFSLNATAVTYPEPPNPFYYITD
                     YTKNTLSQQEWRTLEDALIANRAKTSSQIAVVIIPSTEGEAVSTYAHTLFNKWGIGRS
                     KNNENNGILLLIAKNDRKLFIATGRGLEGALPDAIASSIIRNDITPFFKQEQYAAGIA
                     RGLSAIMAAINGEYAPYASYGEEMQEFDDIDGLLFFLFAGIVIFLIFRPRGNSTYISP
                     SSMDQLGQILRESQRRGGGFGGGLGGGFGGGFGGGFGGSRGSGDSFGGGSSGGGGAGG
                     SW"
     gene            complement(5188..5667)
                     /gene="ispF"
                     /locus_tag="PWY43_000584"
     CDS             complement(5188..5667)
                     /gene="ispF"
                     /locus_tag="PWY43_000584"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDL2135490.1"
                     /translation="MIRIGHGFDVHAFGEDRPLMIAGVEVPYHTGFIAHSDGDVALHA
                     LTDALLGAAALGDIGKLFPDTDMQYKNADSRGLLIEAYRQVQEQGYQVGNVDVTIIAQ
                     APKMRPHIDKMRQVIADDLQCDISQVNVKATTTEKLGFTGRGEGIACEAVALLLKRA"
     gene            complement(5669..6352)
                     /gene="ispD"
                     /locus_tag="PWY43_000585"
     CDS             complement(5669..6352)
                     /gene="ispD"
                     /locus_tag="PWY43_000585"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL2135491.1"
                     /translation="MKRKIIAVIPASGVGSRMQAEKPKQYLQLKGKTILEHTVSIFLE
                     HPHVEKVIVAVARKDPFYSTLELLKSPKIQLVFGGETRADSVLNALQEINTENAWALV
                     HDAARPCLKRSDLDKLLQINDPQGGILAIPAVDTMKRANGTKIAHTEDRSTLWHALTP
                     QFFPAQALKQALSSAFANKLQVTDEASAMELAGYQPQLVSGRSDNIKITRPEDLALAE
                     FYLTHNLEN"
     gene            complement(6370..6648)
                     /gene="ftsB"
                     /locus_tag="PWY43_000586"
     CDS             complement(6370..6648)
                     /gene="ftsB"
                     /locus_tag="PWY43_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDL2135492.1"
                     /translation="MRLLITFFALLLGFFQYSFWFGKNGWSDYQEVQEEIALLKVENT
                     KLTSRNKLIEAEIYDLKNGVDALEERARLEREMVKENEIFYRIVPKNK"
     gene            6923..8530
                     /gene="pckA"
                     /locus_tag="PWY43_000587"
     CDS             6923..8530
                     /gene="pckA"
                     /locus_tag="PWY43_000587"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL2135493.1"
                     /translation="MLNRIEQELADLGITNVKEIVRNPSYEQLFEEEMKSDLKGYEKG
                     VLTNLGAVAVDTGIFTGRSPKDKYIVLDETTKDTVWWTSDAAKNDNKPMTQSTWQSLK
                     TLVTDELSNKRLFIIDAFCGASPEHRINVRIITQVAWQAHFVKNMFIRPTEEELKDFK
                     PDFVVMNGSQVTNPNWKEQGLNSENFVAFNITEGVQIIGGTWYGGEMKKGMFSMMNYF
                     LPLKGVASMHCSANVGEEGDVAVFFGLSGTGKTTLSTDPKRKLIGDDEHGWDENGVFN
                     YEGGCYAKTINLSIENEPDIYRAIRRDALLENVVVREDGTIDFDDKSKTENTRVSYPI
                     YHIDNIAVPSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERG
                     ITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDT
                     RGIIDAILDGSIEKAEMSTLPIFNLAIPTALPGVETHILDPRNTYEDKAQWQAKAEDL
                     AGRFVKNFEKYATNEEGKALIAAGPKL"
     gene            8623..9624
                     /gene="bioB"
                     /locus_tag="PWY43_000588"
     CDS             8623..9624
                     /gene="bioB"
                     /locus_tag="PWY43_000588"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDL2135494.1"
                     /translation="MTTFQLRLKAKDELTPHPTAQYWRKCQVEELFDLPFMDLVFRAA
                     QVHRENFDANAIQLSTLLSIKTGGCPEDCEYCPQSARYDTGIEKQTMLDVETIVEKAK
                     IAKERGASRFCMGAAWRGPRPKDIKVVSEIINAVKSLGLETCGTFGMLEEGMAEELKQ
                     AGLDYYNHNLDTDPERYNKIIHTRNHEDRMDTLGKVRNAGLKVCCGGIVGMNETRPER
                     AGLIASLANLDPQPESVPINQLIKVEGTPLTNAEDLDWTEFVRTIAVARITMPQSFVR
                     LSAGRTTMPEAMQALCFMAGANSIFYGDKLLTTENPEEDHDRQLMDKLDLYPLQYIA"
     gene            9780..9877
                     /gene="ffs"
                     /locus_tag="PWY43_000589"
     ncRNA           9780..9877
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWY43_000589"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            9912..10619
                     /gene="gloB"
                     /locus_tag="PWY43_000590"
     CDS             9912..10619
                     /gene="gloB"
                     /locus_tag="PWY43_000590"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDL2135495.1"
                     /translation="MLQITPIPALKDNYIWAIRNGQDVIIIDPSEHQPALNFIAKNAL
                     NLTAILITHNHHDHTDGVSGIVEQYPNIPVYGPQEVAEFANVIVSPEQHISLFGYDVR
                     IIESGGHTAGHISYLFGYDYLFCGDALFSGGCGRVFTGDYQAQFDALQRFKNLPNSVK
                     AYPAHEYTKSNLKFAEAVLPPSCVLSEYQEQVDLLRSQNKPTLPTTIGLEMQVNPFMQ
                     AVDLAEFIALRQQKDNF"
     gene            10778..11491
                     /gene="uppS"
                     /locus_tag="PWY43_000591"
     CDS             10778..11491
                     /gene="uppS"
                     /locus_tag="PWY43_000591"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDL2135496.1"
                     /translation="MNFDPQNMPKHVAIIMDGNGRWAKQKGKLRIFGHQNGVKAVRSA
                     VSFAAKHHIQVLTLYAFSSENWSRPETEVSALMSLFMKALDSEVKKLHKNNIRLKIIG
                     DKSAFSENLQKRIAESEKLTENNTGLILNIAANYGGYWDIVQAAQQLAVHVKSEQLAV
                     EQITPALFQQHLVTENQPQVDLLIRTSGEQRISNFLLWQIAYAELFFSPVLWPDFNDE
                     AFSDAIYAYQQRERRFGGC"
     gene            11515..12384
                     /locus_tag="PWY43_000592"
     CDS             11515..12384
                     /locus_tag="PWY43_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL2135497.1"
                     /translation="MLKERVLSAIIMIIAVLAAIFWLSPLPLTLVLSAIVVAAMWEWA
                     QFAGFKHPVPRAIVAMVTICLLLFAIVANTDYIRATRFITDETTPILFIGCIWWLIAL
                     VLVATYPNSAKLWEKSVAAKFLFGLATLVPFLIGTLALRFHNYAFDPYQGTYLFLYVC
                     LLVWGADSGAYFAGRAFGKRKLAPKVSPGKSWEGVIGGVLTSSLIAFTFLQLTPANVF
                     GKALATTPFILVSVGTVAISVLGDLAESMFKRQAGIKDSSQLIPGHGGILDRIDSLTA
                     AVPFFATFFFFVL"
     gene            12388..13698
                     /gene="rseP"
                     /locus_tag="PWY43_000593"
     CDS             12388..13698
                     /gene="rseP"
                     /locus_tag="PWY43_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL2135498.1"
                     /translation="MTSIIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVL
                     FKKKDKQGTEFVFSLIPLGGYVQMWNGETDINAPAQQALNTKSVLQRAFIIIAGPAAN
                     FIFAILAYWVVFIAGIPTVKPVIGEILPNTIASQARIPTELEITKIGHQSIQDWESAS
                     LALVGFVGNKNVPLEGNINGNSTQKYELDLSNWSIDSAKENPLTALGIRPKSAKVTAE
                     IKQVVENSPASQMGIQAGDRILQINQQPFNWFNLVELVQAGKPIELKIEQRGQIKDLV
                     VQPEKKDERYIIGVIPSYEPLADKYRTELKYDILTAFYKSIEKVWSLTQTILQFIGNL
                     ISGDLSIKNLGGPISMAKGAGATAEIGLVYYLSFMALISVNLGVMNLFPLLPLDGGQL
                     VLLAVEAIRGKALSEKIQLKFQQIGFAFVLSLMLFAFANDIIHF"
     gene            13813..16194
                     /gene="bamA"
                     /locus_tag="PWY43_000594"
     CDS             13813..16194
                     /gene="bamA"
                     /locus_tag="PWY43_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDL2135499.1"
                     /translation="MKKLLLSSLLIANGVVAAPFVVKDIRVEGVQPTTGESIISSIPV
                     RVGQTATDTDVSNVVRHLFSQQRFADVRATREGNTLVIKVAEKPIIGKVEIEGNQAIP
                     KDALEQNLKANLINQGEIFDAAKLEAFKEGLIEHYHSTSRYEAKIDTSVANNTEGGVN
                     IKLAINEGEVAKAKTIKFEGNNAFSDKKLLENLDIQPDVSWWNIFESSKFEQTAYQKD
                     LETLRDFYLNRGYAKFAIQDTDVQFNDNKTEVALTYKLDEGSQYHVSEMRIIGNTANM
                     DSQLNALLKEFKPGQLFRKSDLTQIEEEIKSILGDAGYGSAKVDIYPQFNEENKTVKI
                     SFVVDAGRRLYVRKIRFEGNGVTADSTLRREMRQQEGAWLSTNKASLGKARLERTGFY
                     ESVDMSMPNVEGTADQVDVVYKIKERNTGSINFGIGYGTENGISYQAGIKQDNFLGMG
                     STISLNGTRNNYGTSVNLGYTEPYFTKDGVSLGGNIFYEDYDNSDNDSVASYKRQTYG
                     INGTLGFPVNENNSYYLGLGYTHDKIKNAAREYTREKYVKSMNFEFDPATDYYKKIKA
                     DDFDFSLGWNYNSLNRGYFPTEGTTANIGGKITIPGSDNKYYRVSADFRNYYPLNREH
                     KWVISTKASIAYTNGFGGKEVPFYQLYTAGGIGTLRGFSYGAIGPQAIYYNTKSGKFD
                     SMKNDIIGGNALAAASLELITPTPFVSEKYQHNVRTSLFVDAASAWHTKWKKDEYTNL
                     PDYGDFKRFRASAGIAFQWMSPIGPLSFSYAKPIRKYDNDDIEQFQFNIGSSF"
     gene            16274..17065
                     /locus_tag="PWY43_000595"
     CDS             16274..17065
                     /locus_tag="PWY43_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDL2135500.1"
                     /translation="MKKLFKMTGLAVALASTTGIANAADTIGFVDPSYVLQNHPVLLD
                     ASAKFDKFMKESQEKFAADEKKLADENKTLTAERNKINEDAQKLQNEQKNVEASIKKK
                     VAALEKDAPRLRSKEIQARQNAIQKEANAFQAKVTAIQKREADFAKKAEAFQKRADEF
                     QKRIEQANKENGGINPQEAQKQAVDEVNATIKEIAKSKGYTLVLQPQVALYAEDEGKD
                     ITEAVLDAIVKKHPEIKIEKPATEAQPATEKETKAEETKPEEAKK"
     gene            17065..18090
                     /gene="lpxD"
                     /locus_tag="PWY43_000596"
     CDS             17065..18090
                     /gene="lpxD"
                     /locus_tag="PWY43_000596"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDL2135501.1"
                     /translation="MANFRLIDLAEHIGGTLRGNADLAIQSIAALDKADSSQITFISN
                     AKYRENLTACNAGAIIVSPADVEFCRADQNLIIVANPYVAYAQLAQYMDSTPKAASEI
                     HPSAVISPEAKLGNNVSVGANAVIESGVELGDDAVIGAGCFVGKNSKIGARTQLWANV
                     SVYHNVQIGADCLIQSSTVIGSDGFGYANDKGQWIKIPQTGGVIIGNRVEIGACTCID
                     RGALDPTVIEDNVIIDNLCQIAHNVHIGFGTAVAGGVIMAGSLKVGHFCQIGGASVIN
                     GHMEICDGAIITGMGMVMRPITEKGIYSSGIPLQTNKEWRKTAALVMNIDEMNKRLKS
                     LEKRFND"
     gene            18119..18577
                     /gene="fabZ"
                     /locus_tag="PWY43_000597"
     CDS             18119..18577
                     /gene="fabZ"
                     /locus_tag="PWY43_000597"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2135502.1"
                     /translation="MTTENREPNIIEVTEIMDMLPHRYPFLLVDRVTDYEEGKWLKAI
                     KNVTFNEPCFTGHFPGAPIFPGVLILEAMAQATGVLAVATYGKLSEDELYYFAAIDNA
                     RFKRPVVPGDQLQLEVEFLKEVRGITKFTGKAYVDGKLVCEADLMCARRK"
     gene            18611..19405
                     /gene="lpxA"
                     /locus_tag="PWY43_000598"
     CDS             18611..19405
                     /gene="lpxA"
                     /locus_tag="PWY43_000598"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDL2135503.1"
                     /translation="MRLIDSTAKISPLAIIEEGAEIGAHVEIGPFSVIGKDVKIGART
                     KIHSNVVINGMTEIGEDNHIFQFASIGEINQDLKYQGEPTKVVIGNRNRIRESVTIHR
                     GTVQGGGVTKIGDDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
                     IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHARPFGINFEGLKRRGFDKPTMHAIR
                     KVYKLIYSSGKTLEECLVEIEQIAATEPAIAIFKQFFKRSTRGIIR"
     gene            complement(19443..21443)
                     /gene="mnmC"
                     /locus_tag="PWY43_000599"
     CDS             complement(19443..21443)
                     /gene="mnmC"
                     /locus_tag="PWY43_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDL2135504.1"
                     /translation="MGKLTFATLTFNDADTPVSEQFDDIYFSTQDGLAESYYVFQEGN
                     QLWEKWQSHPKEAFVIAETGFGTGLNFLAVADKFQQFRAAFPQSKLKRLYFISFEKFP
                     LTASQLADIHQRYPQFATLSENLTACWQPRQIGCQRYHFEQVYLDLWFGEIAENLPQL
                     GDLYSGTIDAWFLDGFSPDKNLEMWNEQLYLQMFRLSANGGSFATFTASSMVRRGLEA
                     AGFAVKKRKGFGKKREMLWGEKPLDSQVSEHNYPYFYAKNETADDIAIIGGGVASFFV
                     ALSLLEKGKQVTLYCKDETLAINASGNLQGAIYPQLSDDDERNVQFYIHSFDYALQRL
                     QQLKSNIEFEHNLSGVVLYAYNEKTAKKLEKMVEQTADDTLLKSCSQAELSEKLGLSV
                     PNSGAFIPQAGWLSPVQLVRNGFTWLEQKGLKIVLNHKVEHLTFSNGMWQWQTRGETV
                     RHQAVVLANGHTLNEFSQANGIPLYPVRGQVSQIPTTSELQKLKCVLCYDGYLTPMSA
                     YGTHCIGASHIRDNAETEFSLSEHWENVAKLQQNVTVCEWTQGIDLSENKAKVGIRAA
                     FRDRVPMVGAVHHFEQQKSQYANLYNQLIRKQAVENAEIFTNLYMVNGLSSRGLTTAP
                     LLGELLASLIVNEPVPISEDIWHNLSPNRAWIRKLLKGSTVA"
     gene            complement(21668..23104)
                     /gene="trpCF"
                     /locus_tag="PWY43_000600"
     CDS             complement(21668..23104)
                     /gene="trpCF"
                     /locus_tag="PWY43_000600"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDL2135505.1"
                     /translation="MQNIVQDNAQPNQPTILQKIVQDKAIWVAQKEQAFPLSEFQHKI
                     VKSDHDFYAALAKGTHQEPAYILECKKASPSKGLIRAEFDLDSIAQVYKNYAAAISVL
                     TDEQYFQGDFRYIDQVKRQTTQPILCKDFMISAYQVYLARFYNADAILLMLSVVNDET
                     YRELATLAHELGMGVLTETSTEAEFERALALGAKVIGVNNRDLHTLTIDMNRIVRLVE
                     KYQAQIPADVCLISESGINDHQQVKNIKPYVHAFLIGSSLMGSADLNNAVRAVIFGEN
                     KVCGLTRVQDVKAVYEQGALYGGLIFAEGSPRQLSLRQAQELVVNAPLRYVGVFQNQA
                     VEFVEKMAKQLELFAVQLHGSEDEQYIAELAEKLGSKTQIWKALSVDVEVENLEFNDN
                     PQIARYVLDSKVGNQQGGTGKTFNWALIPETLKQKALLAGGIGTENIEQALAQGCVGL
                     DLNSGVESAKGVKDLQKLTACFGKIIQA"
     gene            23192..23503
                     /locus_tag="PWY43_000601"
     CDS             23192..23503
                     /locus_tag="PWY43_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDL2135506.1"
                     /translation="MKSQVIQHLSYLEIALRVNQLWEATPPSPEALANEQPFCVETLT
                     PTQWLQWIFIPRMRAILDANADLPRNFAITPYLEEALKNEGYLQAICEPLLGLEKLLK
                     D"
     gene            23657..24394
                     /gene="truC"
                     /locus_tag="PWY43_000602"
     CDS             23657..24394
                     /gene="truC"
                     /locus_tag="PWY43_000602"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDL2135507.1"
                     /translation="MTLDILYQDNSLIAINKPAGVLVHRSWLDKHETVFAMQTLRDQI
                     GQHVFPIHRLDRPTSGVLLFALNSETARIMSEQFEQHLVHLVQKTYLAVVRGYLQGSA
                     QIDYPLKVILDKIADKFSQEKEAQAAITDYKGLATVEMPYPVGKHQTSRYSLVELSPK
                     TGRKHQLRRHLKHLFHPIMGDSKYGDLHQNRALVEKSGVSRLMLHAHKLQFQHPKNLQ
                     KIEIIASLDEQWQRLFAFFDWNFTQYY"
     gene            24412..24576
                     /locus_tag="PWY43_000603"
     CDS             24412..24576
                     /locus_tag="PWY43_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDL2135508.1"
                     /translation="MDNSLLSLTHEQQQAAVEEIQKLMAQGISAGEAIQIIANQLRDK
                     YQILEKSNNE"
     gene            24569..25045
                     /locus_tag="PWY43_000604"
     CDS             24569..25045
                     /locus_tag="PWY43_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135509.1"
                     /translation="MNKLWTVLALTITSHYSFAAAEKIVTKDLPAEGKPAVVLQVFDE
                     SEKIAKPIKGNKLFISKKQTRLCWHSINLPVQNKVTIAEAFYAPAAMKLASPGSKINS
                     SADKKNHTIITEINLANNQVLNRCWGFDKTDPVGKYKMEIQINDHIFKGLEFEIVK"
     gene            complement(25082..25933)
                     /locus_tag="PWY43_000605"
     CDS             complement(25082..25933)
                     /locus_tag="PWY43_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL2135510.1"
                     /translation="MENQKQAEIELKIMLEQQNVALVENYLNQTSPFPVLAHQTELLG
                     NTYYDTPEQFFATQKMGLRVRSVNQQYEITLKTQGEIVGGLHIRPEYNLALPNSQPDF
                     QTLVEKYQLPFENSEQIAESLQAIFSTDFTRKTWLVQVGMSDIEVALDQGIIKNHFGE
                     EPICELEFEIKQGNLGDLFTLVEAMPKADGMWLSNLSKAQRGYLLGQAVKWEQKIATA
                     MDGENSEKLTQLLADFICVGNESLEVLVRFNQCTQQQFSNWQQARAFVKSKDYLLHNL
                     TNLKTMI"
     gene            25994..>26965
                     /locus_tag="PWY43_000606"
     CDS             25994..>26965
                     /locus_tag="PWY43_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDL2135511.1"
                     /translation="MFSKIHHKLNNFVQRGLDNHLRLAVTGLSRSGKTAFITSLVDQL
                     LHINKGGNSHLNLFAPARHGQILSVKRVEQGDLTIPRFEYDKNRQSLENQPPQWPEST
                     TGISEIRLAIRYQRQHSLLRHIKETGTLYLDIFDYPGEWLLDLPLLSLSYKEWSQTQQ
                     TIHKDERAELATPWLQAVKKLDLFAKTDENLLAEFSQQYTDYLLACKAQGMQYIQPGR
                     FVLPTENAKGAPVFQFFPLLDLSEAEWEKLEQSDRNSIFHTLKKRYEHYQKKIVKPFY
                     EDYFSQFDRQVILADCLTPLNYGYNEFMEMKLGLQQLFKHFHYGNRSL"
BASE COUNT         8321 a         5534 c         5354 g         7756 t
ORIGIN      
        1 caagtaggag caggaaatcc aatttccgcc gaagaagtcg cagcggcaga tttagtcttt
       61 gtagctgccg atattgatgt cgatttggaa aaattcaaag gcaagccgat gtatcgcacc
      121 tcaaccggtt tagcattgaa gaaaaccact caagaatttg aaaaagcctt tgctcaagcg
      181 aaaatttacg aaggtggtgt ggcaaaagcg gataatgctg aagttccaac ttctgagaaa
      241 aaagggttat ataaacactt gatgaccggt gtatcacata tgttaccgct ggtggtagca
      301 ggtggcttgc tcatcgctat ttcctttatg ttcggaattg aagcctttaa agatgaaacc
      361 atcgcaggcg gcttgccgaa agccttaatg gatattggtg gcggtgcggc attccactta
      421 atgattgcgg tattcgcagg ctatgtagcg ttctcaattg cagaccgtcc ggggttagct
      481 gtcggcctta tcggcggtat gcttgctacc accgcaggag cagggatttt aggtggtatc
      541 gtagccggtt tcttagccgg ttataccgta aaattcttaa atggtgcaat tcagctaccg
      601 gcaagtttaa cctcacttaa acctattttg attttaccac tcttaggctc agcgattgtc
      661 ggcttactga tgatttatgt catcaaccca ccggtgaaag cattaatgga tgccttagtg
      721 gaatggttaa acacaatggg gcaaaccaat gcaattattt taggtattat tctcggtgcg
      781 atgatgtgta ccgatatggg tggtccggtg aataaagcgg cttatacctt tggtgtgggt
      841 ttaattgctt cacaacaata tatgccaatg gcagcggtga tggcggcagg tatggttcca
      901 ccaattggta tggcgattgc gaccctgatt gctcgtagca aattcaacac caaccaacgt
      961 gatgcaggta aagcttcatt cgtgttaggt ttatgcttta tttcagaagg ggcattaccg
     1021 ttcgtggcgg cagaccctgt gcgtgtaatt gccagctcaa tcttaggcgg tgcaaccgcc
     1081 ggtgcaattt caatggcact aggcattacc ctacaagccc cacacggcgg tttattcgtg
     1141 attccattcg tttcacaacc gttaatgtac ttagctgcta ttgccatcgg ctcattagtc
     1201 accggcgtag tctatgccgc tatcaaacaa aaagcagaat aaagcacaaa cacaaataca
     1261 aacaaaaacg gagcgaaacg ctccgttttt atatcatatt ggtacttact gaattctatt
     1321 tcatcgaaat atcaagtcta aaatattatt tttgcgatcg agatcgaaaa aatcattttc
     1381 agcactttac gaataatatc aactcgttat gattttgcta cgaggagata ctatgcagtt
     1441 tattgaattt accgatcctg atagtggaat ttcttatcaa tacagtgaat ttactattgc
     1501 taatgtcgca tttatcatta atttctgctc agatgccgat gttatatcga ctttgtccgc
     1561 attaggcaaa gacatcacaa actatataaa cacctattcc tgttgcacaa tcaaatttat
     1621 ggcgaaagag catctagaaa atagcggttc taatattgac atttatgccc ccgcagcgaa
     1681 tcatcagttc aaacgtaaag aaataattgc tttacaagaa acccttgagc gtttattatt
     1741 tgagcattat gtacgtttta caccagaaag ctatctcttc attgccgaga gagattcact
     1801 taaccgaatg tatcagcata tgtgtgtgcc acgatgtgat tttatgcaat ctttccaagt
     1861 ggtttatccc ttgggcgtta atcaagattg tttcatttta attacgccaa aaggaaatct
     1921 aaaatgaatc aacaacgtcc agtttccgtt cgtttaaagc aagaaggctt aatcgctttt
     1981 aagcaagcct atcagcgagc aaaaaaagca ggtctattga aaccggtcaa ataatcttta
     2041 aaccgcttgc tctacaagcg atttattttt ccttaattat cacaccttgc tacgaaaact
     2101 atcccaactc aataatccaa tcgaaaccag caaacagacc atcaaaatat aggaattggc
     2161 atcgagtttt tcgccgatgg caaaagaaat cagcaacatc accaacggtt caacatagcc
     2221 cagtaaacct aatacattaa tcggtaataa attgctcgag aggatataaa gaataaaagc
     2281 actgccactt accagcccca gcaatccgag gtagaaataa atttgcggat tttctgccac
     2341 tattggcgtc atttccactt ggctgagata ataaagtgca acgggtaata ccagtaacaa
     2401 ctccacaaaa aaagtagcta acgtgttgat ttgaaaataa cggcgaaggg taatataaag
     2461 cggataaccc aacccagcta accaagtcgc ccacgaaatc gtgctgttaa aaaacaaact
     2521 actgccaaca ccgatgactg cgaaaagtag agaaagccat ttcaggcggg taaaatgttc
     2581 tttaaacacc atttttccta gagccaccgc cacaatcggt aaaagcaagt aaccaaacga
     2641 cacctgcaac gcctgcccgt gattaggagc ccataaaaac agccaaagct gcacgccggt
     2701 gttgagagca aggagcaaaa tgaccaatac aagcggtgga ttttgtcgaa ttttttgcaa
     2761 aagatccgca aaagccgatt gctgtttaaa caatataatc gccagcaaaa taaacggaat
     2821 gactaccact atccgaaagc cgaacatcgc ttctccttct aacgggcgaa gtaatatcgc
     2881 aaaatagtaa actgctccaa acagcacatt ggctgcaagc gaagctaaaa ttcctctgag
     2941 catttggctt tcctcttatc tacaatcaaa caacaagcgg tcgactttga acaaaaattt
     3001 acaaatctta tctacgcccc atagtatgaa aaaatttgta agtacgctag ctatatccgc
     3061 tttttcccga tacaatgcac ccaatttttc actcttttat ttataggatt tcataatgaa
     3121 aaagattttt tggattttac tgatcgccgt gcttgcagtt ggcggttatt ctttaacaaa
     3181 atacaacgat ttaatgcgtg cagaagaaga catcaactcc gtatggggga atgttgaatc
     3241 tgcttaccaa cgccgagcgg atttaattcc aaacttagtg aataccgtaa aaggtcaggc
     3301 taactttgag aaagaaacct taacatctgt aattgaagct cgtgcaaaag cgactgcggt
     3361 aactattgac ccaagcaatg ccaccgaaga gcaaatggct aaattccaag aagcccaaca
     3421 aggtgtaaac tctgcccttt cacgtttatt ggtttccgtt gagaaatacc ctgaattaaa
     3481 agctcacgaa gcgtttttaa atctgcaagc tcaattagaa ggcacggaaa accgcattaa
     3541 cgttgaacgc aataacttta acgaaaaagt gaaagagtac aacaaacaag tgcgtgaatt
     3601 cccgactaaa ttagcggcaa tgattatggg ctttaaagca aaaccgcaat ttaaatctga
     3661 agcaggttct gataaagcac caaccgtaaa cttcaactaa ttttagtttt aatttattca
     3721 tattaaaagc aataagctct ttcaaattcg gtgcttattg ctttcttttt aaggggaaca
     3781 aatgggatta ttttcttttc tctccaacaa attaccgata gatacgcaac gcattgaaca
     3841 agcaattgcc gatttagagc aaaaaacctc tgccgaattg cgtgtggtgg tggaacgaaa
     3901 agccaaaatc ggcaaaaccg acaacgccgc tatgttacga gcaagtgagc ttttcgatga
     3961 attaaaaatg cgtgaaacgg ctgaacataa cggtgtgttg atctaccttt cattcaagcc
     4021 tcactacgct gcgattattg gcgatgaagg cattcatcaa aaagtcgata acgatttttg
     4081 gcaaaaaatc tatgccacaa tgtgtttgga ctgccaacaa ggcgattaca cccaagccat
     4141 ttgtgatgca atcaaaatgg tggaagctcc gctcgctcac catttccctt atcaggctga
     4201 tgataaaaac gagctttcaa acgaggtggt agtcaaatga tgaaatccct ttgcaaattt
     4261 ccatctattt tcaaccgctt gttattggtg tgcttggctc tgtttagcct aaatgccaca
     4321 gcggtgacct acccagagcc gcctaatccg ttttattata ttacggacta caccaaaaac
     4381 accttgagcc aacaagaatg gcggacatta gaagatgcct taattgctaa ccgtgccaag
     4441 acaagttcgc aaattgccgt ggtgattatt ccaagcacgg aaggcgaggc agtttcaacc
     4501 tatgctcata ccctgtttaa caaatgggga atcggacgga gcaaaaataa cgagaacaat
     4561 ggtatattgt tattaattgc caaaaatgat cgcaaactct ttattgccac cggacgtggg
     4621 cttgaaggag cgttacctga tgcgattgca tcgagcatta tccgcaatga catcacgcca
     4681 ttcttcaaac aagagcaata tgcagccggt atcgcccgtg gcttatccgc cattatggca
     4741 gccattaacg gggaatatgc cccttatgca agttatggcg aagaaatgca agaatttgat
     4801 gacatagacg gcttactctt tttcttgttt gccggcattg tgattttcct aattttccgc
     4861 ccacgaggaa acagtaccta tattagtccc agctcaatgg atcaactagg acaaatacta
     4921 agagaaagcc aacgcagagg tgggggcttt ggcggtggtc tcggaggtgg atttggcgga
     4981 ggcttcggtg gtggttttgg cggaagcaga ggcagtggcg acagcttcgg tggtggttca
     5041 tcaggcggtg gaggcgctgg aggaagctgg tagtttccgc ccaatttaaa acaatacaag
     5101 cggtcatatt tctcaaaaaa attgcgtatt tttcgctaaa cgcacaaaac gctaatgaaa
     5161 ataagaccgc ttcctcactt ctctaagcta tgcccttttt agtaacaatg ctacggcttc
     5221 acaagctatg ccttcacctc gcccggtaaa ccctaatttt tctgttgtag tggcttttac
     5281 attcacttgt gaaatatcgc attgtagatc gtctgcgatc acctgtctca ttttatcaat
     5341 atgcggtcgc atcttcggag cttgagcaat aatggttaca tcgacgttac cgacttgata
     5401 gccctgttct tgcacttgtc ggtaagcttc aatcaacaaa ccacgactat cggcattttt
     5461 gtattgcata tcagtatcag ggaataattt gccaatatca cctaaagctg ctgcgccaag
     5521 caacgcatct gttaaagcgt gcagcgctac atctccgtca ctatgcgcaa tgaagcctgt
     5581 atgataaggc acttcaaccc cagcaatcat cagtggtcta tcttcgccaa aagcgtgaac
     5641 atcaaaaccg tgtccaatac gtatcatttt agttctccaa attatgtgta aggtaaaatt
     5701 ccgctaaggc taaatcttca gggcgagtga ttttgatatt atcacttcta cctgaaacca
     5761 attgtggctg gtagcctgca agctccattg cagatgcttc gtctgttacc tgaagcttat
     5821 tggcaaaagc agaggataat gcttgtttta atgcttgagc aggaaaaaat tgtggggtta
     5881 aagcgtgcca taaggttgag cgatcttctg tgtgtgcgat tttcgtgccg tttgctcttt
     5941 tcatcgtatc taccgctgga atggcgagaa tgccaccttg cggatcgtta atttgcaaaa
     6001 gtttatctaa atctgaccgc ttgaggcaag ggcgagctgc atcgtgaact aatgcccaag
     6061 cattttcagt gttgatttcc tgtaaggcat ttagcactga atctgctctc gtttctcctc
     6121 caaaaacaag ttggatctta ggggatttta ggagttccaa tgtggaataa aaagggtctt
     6181 ttcttgccac cgctacaatc actttttcaa catgagggtg ttcaagaaaa atagacactg
     6241 tatgctctaa gatagtctta ccctttaatt gtaaatattg ttttggtttt tctgcttgca
     6301 tccggctacc aactcctgac gccggaatta ctgcaataat ttttcgcttc ataatatatc
     6361 aattatgtat tatttgtttt ttggtacaat tcggtagaaa atctcgttct cttttaccat
     6421 ttctcgttca agtcttgctc tctcttcaag tgcatctacg ccgttcttta aatcgtaaat
     6481 ttcggcttca attagtttat tgcgtgaagt aagctttgtg ttttctactt ttaataaagc
     6541 aatttcttct tgcacttctt gataatcact ccaaccattt tttccaaacc aaaagctata
     6601 ttggaaaaag ccgagtaata gggcaaaaaa agtaattaat aaacgcattt attttctctt
     6661 atatgaaata gtaatgaact aagcgggttg attttacctt gaatttgcaa aaaattctaa
     6721 aaatcttacc gcttgttact taaacaggcg atggatagtg ctaaatgtat tcgaaaaatt
     6781 gatataaaac aaacaaatgc gtattttaaa gcggtttttt taaactaaat gtcaaaaatt
     6841 tgaagtatgt aacatttttt atttttcaat tttcgtataa tcaacccagt tttaaattta
     6901 taactattat gaggtaacca ctatgttaaa tcgtattgaa caagaacttg cagacttagg
     6961 cattacaaat gtaaaagaaa tcgtgcgtaa ccctagctat gagcaactgt ttgaagaaga
     7021 gatgaaatcg gatcttaaag gttatgaaaa aggcgtccta acaaatttag gcgcagtagc
     7081 ggtagatacc ggcattttca cggggcgttc gccgaaagat aaatatatcg tattagatga
     7141 aacgacaaaa gataccgtct ggtggacatc tgatgcagct aaaaatgata ataaaccgat
     7201 gactcaatct acttggcaaa gtttaaaaac cttagtgacc gatgagttat ctaataagcg
     7261 tttatttatt attgatgctt tctgtggtgc aagccctgag catcgcatca atgtgcgtat
     7321 tattacccaa gttgcgtggc aagctcactt tgtgaaaaat atgtttattc gtccaacaga
     7381 agaagaatta aaagatttca aaccggattt tgtggtaatg aacggttccc aagtaactaa
     7441 cccgaactgg aaagagcaag gcttaaattc agaaaacttt gtggcattta atatcacaga
     7501 aggtgttcag atcattggcg gtacttggta cggtggtgag atgaaaaaag gtatgttctc
     7561 tatgatgaac tacttcttac cattaaaagg tgtcgcttca atgcactgtt cagccaatgt
     7621 gggcgaagaa ggtgatgtgg cggttttctt tggtttatca ggaaccggta aaacgacact
     7681 atcaaccgat ccgaaacgca aattgattgg tgacgatgaa cacggttggg acgaaaacgg
     7741 cgtatttaac tatgaaggtg gttgctatgc gaaaaccatc aacctttcta ttgaaaacga
     7801 gccggatatt tatcgtgcaa tccgccgtga tgcgttatta gaaaacgtgg ttgtgcgtga
     7861 agatggcact attgattttg atgataaatc aaaaacagaa aatacccgtg tgagctatcc
     7921 gatttatcat attgataata ttgccgtgcc gtcaaaagca ggacacgcta aaaaagtgat
     7981 tttcttaacg gcagatgcat tcggcgtatt gccaccggtg tctaaattaa cgcctgaaca
     8041 aactaaatac tacttcttat caggctttac cgcaaaatta gcaggtacag agcgtggtat
     8101 tactgagcca accccaactt tctctgcttg tttcggtgcg gcgttcttat cactccaccc
     8161 gacacaatat gctgaagtgt tagtgaaacg tatggaagca gccggtgcgg aagcctattt
     8221 agtgaatacc ggttggaacg gcacaggtaa acgtatttcg attaaagata cacggggtat
     8281 tattgatgcc atcttagacg gctcaattga gaaagcggaa atgagtacat taccaatctt
     8341 caatttagca attccaactg cgttaccggg tgtagaaacc cacattttag accctcgtaa
     8401 cacttatgaa gacaaagcac aatggcaagc taaagcagaa gatttagctg gcagatttgt
     8461 gaaaaacttt gaaaaatatg cgaccaacga ggaaggcaaa gcattaattg ctgctggtcc
     8521 gaagttataa gttgtaatta taagttataa tcgctaaaaa attagtttag gactaaacct
     8581 tgaatgaggt ttagtccttt tgtataaagg agaagtacaa taatgacaac attccaatta
     8641 aggctgaaag ccaaagatga gcttacccca cacccaacag cacaatattg gcgaaaatgc
     8701 caagtggaag aactatttga tctgccgttt atggatttag tgtttagagc ggcacaagtt
     8761 caccgtgaaa attttgatgc taatgcgatc cagctttcaa cgttactctc aattaaaaca
     8821 ggaggctgtc cggaagattg cgaatattgc ccccaatctg cccgctatga tacgggaata
     8881 gaaaagcaaa caatgcttga tgtagagacg attgtagaaa aagctaaaat tgccaaagag
     8941 cgtggagcaa gccgcttttg tatgggggct gcgtggcgag gacctaggcc taaagatatt
     9001 aaagtagtct cagaaatcat caatgcggtg aaatcattgg ggctagaaac ctgtggtact
     9061 ttcggtatgc ttgaagaggg aatggcagaa gaattaaagc aagcaggact ggattactac
     9121 aaccataatt tagataccga ccctgaacgc tataacaaaa ttatccatac ccgaaatcac
     9181 gaagatcgaa tggacacttt aggtaaagtt cgcaatgccg gcttgaaagt ttgctgtggc
     9241 ggtattgtgg gaatgaacga aacccgcccc gagcgagcag gcctaattgc aagcctcgct
     9301 aacctcgatc ctcaacctga aagcgtaccg attaatcagt tgattaaagt agaaggtaca
     9361 ccgcttacca atgccgaaga tttagactgg acagaatttg ttcgtaccat tgcggtagca
     9421 agaattacta tgcctcaaag ctttgtccga ctttctgctg gcagaactac tatgccggag
     9481 gcaatgcaag ccctctgttt tatggcagga gcaaattcta ttttctatgg cgataaatta
     9541 cttactacag aaaatccgga agaagatcat gatcgccaat taatggataa attagattta
     9601 tatccgctac aatatatagc ctgagtgcca atgattttcg cctgaaaatt aaagaatcaa
     9661 gcgttatttt acgcaaattt ctaacataaa ataaccgctt gcaaaattcg ttcttgtctt
     9721 atttttaaat ttatctataa tgtgaggcct tacgcaagct tgcgtaattc gatggggcgt
     9781 tattggttcc tcgcaatggt gttcagctta cttgatcagg ttcggaagaa agcagtctgg
     9841 gttgaaattt ccgtgtgccg agggtggctg gtaatgcccc accctttttt cagttctatc
     9901 aggagaacgt tatgctccag atcactccca ttccggcatt aaaagataat tacatttggg
     9961 caatccgtaa cgggcaagat gtgattatta ttgatccatc agaacatcag ccagcattaa
    10021 actttattgc aaaaaatgcg ttaaatttaa ccgctatctt gattacccat aatcaccacg
    10081 accatactga tggggtgagt ggtattgttg agcaatatcc gaatatccct gtttatggtc
    10141 cgcaagaagt ggctgaattt gccaatgtga ttgtctcgcc tgaacaacat atcagcctgt
    10201 ttggttatga tgtaaggatt attgaaagtg gtgggcatac cgcagggcat atcagttatt
    10261 tatttggcta tgattatctg ttttgtggcg atgcgttatt ctccggcggt tgcgggcggg
    10321 ttttcaccgg tgattaccaa gcccaatttg atgcgttaca acgctttaaa aatctgccta
    10381 actcggttaa agcctacccg gctcacgaat acaccaagag caatttgaaa tttgcagaag
    10441 cggttctacc accaagctgt gtattatcgg aatatcaaga acaggttgat cttttacgtt
    10501 cgcaaaacaa accgactttg ccaaccacta ttggcttgga aatgcaggtg aacccgttta
    10561 tgcaagcggt cgatttagcc gaatttattg cacttcgtca gcaaaaagac aatttttaac
    10621 ctcatatttc taattaagca ttaaagcctc tcactttaat gctttttggt taaataaaac
    10681 ctttgcaaac atcgcttgtc aattccactc tggcttatcc tatggtatag ttacgactat
    10741 ttttagctta atttattatt ttagtgagta aaattctgtg aacttcgatc ctcaaaatat
    10801 gcctaagcac gttgccatta ttatggatgg caatggcaga tgggctaagc agaaaggaaa
    10861 attacgcatt tttggtcatc agaatggtgt aaaggcagtt cgttctgcag ttagttttgc
    10921 agcaaagcat catatccaag ttttaaccct ctacgcattt agcagtgaga attggagcag
    10981 accagaaact gaagtttcag ctttaatgtc tctgtttatg aaagctttag attctgaagt
    11041 taaaaaatta cataaaaata atattcgctt aaagattata ggcgataaat ccgcctttag
    11101 cgaaaattta caaaaacgca ttgccgaatc ggaaaaatta accgaaaaca atactgggct
    11161 aatcttaaac attgctgcca actatggtgg ttactgggat attgttcaag ctgctcaaca
    11221 actagcagta cacgttaaat ctgaacaact tgctgtggag cagattacac cagcattgtt
    11281 tcaacaacat ttagtgacag aaaaccagcc gcaagtggat ttattaattc gaaccagtgg
    11341 cgagcagcgg atcagcaatt ttctattatg gcaaattgct tatgccgagc tgttttttag
    11401 cccggtttta tggcctgatt ttaatgatga ggcttttagt gatgcaattt atgcttatca
    11461 gcaacgtgaa cgccgattcg gtggttgtta atataaaaag aataaggaaa tacaatgctt
    11521 aaagaacgtg tactttcagc gattattatg attattgccg tgctggctgc catattttgg
    11581 ctctcaccac ttcctttaac tttagtgtta tcggcaattg ttgtggctgc aatgtgggaa
    11641 tgggctcaat ttgccggctt taaacaccct gtgccacgtg caattgtggc aatggtgaca
    11701 atctgcttat tactttttgc gatcgttgcg aatactgatt atatccgagc cacccgtttt
    11761 attactgatg agactacccc tatcctcttt attggctgta tttggtggtt aattgccctt
    11821 gtcctagtgg caacttaccc caactcggca aaactgtggg aaaaatctgt tgcagctaaa
    11881 ttcctatttg gacttgctac cttagtgccg tttttaattg gcacgctcgc attgcgtttc
    11941 cataattatg catttgatcc ataccaagga acttatttat tcctttacgt ctgtttatta
    12001 gtttggggag ctgattcagg tgcttatttt gctggcagag cctttggcaa aagaaaactt
    12061 gccccaaaag tatctccggg taaatcgtgg gaaggcgtaa ttggtggcgt actcacctct
    12121 agtttgattg cctttacctt tttacaactt acaccggcaa atgtattcgg caaagcatta
    12181 gccactaccc catttatttt agtttcggtc ggtacggttg ctatttcagt cttaggcgat
    12241 ttggcagaaa gtatgtttaa acgccaagca ggcattaaag acagtagcca gttaattcct
    12301 gggcatggag gtattttaga tcgtattgat agtttaaccg ctgccgttcc gttttttgct
    12361 acattcttct tttttgtact atagaatatg acgtccatca ttgcattttt tattttaatt
    12421 tgcgtactgg tttttgtgca tgaatacggg cacttttggg ctgctcgcaa atgcggggta
    12481 aaggtgatcc gtttttcgat cggatttggt aaagtcttat ttaagaaaaa agataaacaa
    12541 ggaaccgaat ttgttttttc acttattcct ctgggcggct atgtacaaat gtggaatggt
    12601 gaaacggata tcaacgctcc agctcaacaa gctcttaaca caaaatctgt attgcaacgt
    12661 gcatttatta ttatagccgg tcctgctgca aattttattt ttgcgatttt ggcatactgg
    12721 gtcgttttta ttgccggtat tccaactgtt aaacctgtta ttggggaaat tttaccaaac
    12781 actatcgcct cccaagcaag aattcctacc gaattggaaa ttacaaaaat cggtcatcaa
    12841 tctatccaag attgggaaag tgcatcttta gcactagttg gttttgtggg caataaaaat
    12901 gtaccgttag agggaaatat caacggcaac tcaacacaaa aatatgaact cgatttatct
    12961 aactggagta ttgactcagc caaagaaaac ccattaaccg cattaggtat tcgccccaaa
    13021 agtgcaaaag tcaccgctga aattaaacaa gttgtcgaaa attctcctgc aagccaaatg
    13081 ggtattcaag caggtgatcg tattcttcaa attaaccagc agccatttaa ttggttcaat
    13141 ttagttgaac tggtacaagc aggtaaacct attgaactta aaattgaaca acgaggtcag
    13201 attaaagacc ttgtcgtcca accagaaaaa aaagacgaac gctatattat cggggtaata
    13261 ccaagttatg agcctcttgc agataaatat cgtaccgaat taaaatatga tattcttacc
    13321 gcattttata aaagtatcga aaaagtgtgg tctcttaccc aaaccatact ccaatttatc
    13381 ggtaatttaa taagcggtga tttgtccatt aaaaatttag gtgggccaat ttcaatggca
    13441 aaaggagccg gtgcaacagc tgaaatcggc ttagtttatt accttagttt tatggctcta
    13501 atcagtgtca atttgggggt aatgaactta tttccacttt tacccttaga tggtgggcaa
    13561 cttgttttat tggctgttga agcaattcgt ggaaaagcat tatcagaaaa aatacaactc
    13621 aaattccaac aaattggatt tgcattcgtg ctgagtttaa tgttattcgc ctttgccaac
    13681 gatataattc acttttaaca agcggttaaa aaaggtgact tttttgcaaa aagcaccctg
    13741 acatcaaaat ccataatatg gatttacact atattcttaa taattttgca aaacgatcat
    13801 aggataattt caatgaaaaa attattactt tcctctcttt taattgcaaa cggtgtagtt
    13861 gctgcacctt tcgtagtaaa agacattcgt gttgaaggtg tgcaacctac aacaggcgaa
    13921 tctatcattt catctattcc ggttagagta ggtcaaacag caacggatac tgacgtatct
    13981 aatgtcgtcc gccatctatt cagccaacaa cgttttgcag atgttcgtgc aacacgagaa
    14041 ggcaatacgc ttgtcatcaa agtcgctgaa aaaccgatta ttggcaaggt agagatcgaa
    14101 ggtaaccaag cgattcctaa agatgcctta gagcaaaatt taaaggcaaa cttaattaac
    14161 caaggtgaaa tttttgatgc tgccaagctt gaagctttta aagaaggact aatcgaacat
    14221 tatcactcta ccagccgtta tgaagccaaa attgacacat ctgttgcgaa taacaccgaa
    14281 ggtggcgtta atattaagtt agcgattaat gaaggtgaag ttgctaaagc caaaacaatt
    14341 aagtttgaag gtaataatgc cttctctgat aaaaaattat tagaaaactt agatattcaa
    14401 cctgatgttt cttggtggaa tatttttgaa agtagcaaat tcgaacaaac cgcctatcaa
    14461 aaagacttag aaacattaag agatttttat ttaaatcgtg gctatgcaaa atttgctatc
    14521 caagataccg atgttcaatt caatgataat aaaacggaag ttgcattgac ctataaatta
    14581 gatgaagggt cacagtacca tgtcagtgaa atgcgcatta tcggcaatac agccaatatg
    14641 gatagccagc tcaacgccct actaaaagaa ttcaagcctg gtcaattatt ccgtaaatca
    14701 gaccttacac aaattgaaga agagattaaa tctattttag gggacgcagg ttatggttca
    14761 gctaaagtag atatttatcc tcaatttaat gaagaaaata aaaccgtaaa aatcagtttt
    14821 gttgtggatg ccggtcgtcg tctttatgtt cgtaaaatcc gttttgaagg taatggtgtc
    14881 actgcagatt caacattacg tcgtgaaatg cgccaacaag aaggtgcttg gctttccaca
    14941 aacaaagctt ctctaggtaa agctcgttta gaaagaaccg gtttctatga aagtgttgat
    15001 atgtcaatgc caaatgtaga aggtacagcc gatcaagttg atgtagtgta taaaattaaa
    15061 gaacgcaata ccggtagtat taactttggt atcgggtacg gcacggaaaa cggtatcagc
    15121 tatcaagcag gaattaaaca agataatttc ttaggtatgg ggtcaactat cagtctaaat
    15181 ggtacccgta ataactacgg taccagcgtt aacctaggct ataccgaacc ttactttact
    15241 aaagatggtg taagtttagg cggtaatatt ttctatgaag attatgataa ctcagacaat
    15301 gattcagtgg cttcatataa acgccaaacc tatggtatta acggcacatt aggcttcccg
    15361 gtaaatgaaa ataactcata ctacttaggt ttaggctata cgcacgataa aattaaaaat
    15421 gcagcacgtg agtacactcg tgagaaatat gtaaaatcaa tgaattttga gttcgatcca
    15481 gctaccgatt attacaaaaa aatcaaagcg gacgattttg atttttctct cggttggaac
    15541 tacaacagct tgaatagagg ctatttcccg actgaaggaa ctactgcaaa tattggcggt
    15601 aaaattacaa ttccaggctc tgacaataaa tattaccgag tcagtgcaga ctttagaaat
    15661 tattacccgt taaaccgtga gcacaaatgg gtaatttcaa cgaaagctag cattgcttat
    15721 actaatggat ttggtggtaa agaagttcca ttctatcagc tttatactgc cggaggtatt
    15781 ggtacactaa gaggtttctc ttacggtgct attgggcctc aagcaattta ctataataca
    15841 aaatccggta aatttgatag catgaagaat gatattatcg gtggtaatgc gttagctgcg
    15901 gcaagcttgg aattaatcac accaacgcca tttgtgagcg aaaaatatca gcacaatgta
    15961 agaacatctc tctttgttga tgctgcaagt gcatggcata caaaatggaa gaaagatgaa
    16021 tatacgaact tgcctgacta tggtgatttc aaacgtttcc gtgcttccgc cggtattgca
    16081 ttccaatgga tgtctccaat cggaccatta tcattctctt atgcgaaacc aattcgtaaa
    16141 tatgataatg atgatattga acaattccag ttcaacattg gtagttcatt ctaattaacc
    16201 acaagcggtt aataaatgta aaaaatttgc aacttagccg cttatttcaa aactgttcat
    16261 acaaggaaaa caaatgaaaa aattatttaa aatgactggc ttagccgtag ctttagcctc
    16321 tacaaccgga atcgcgaatg ctgcagatac tattggtttc gtagatccaa gctatgtatt
    16381 acaaaaccac cctgtattat tagatgcttc ggcaaaattt gataaattca tgaaagaaag
    16441 ccaagaaaaa tttgctgctg atgaaaaaaa attagcagat gaaaataaaa ccttaacggc
    16501 tgagcgcaat aaaattaatg aagatgcgca aaaattacaa aatgaacaaa aaaatgtaga
    16561 agcttctatc aagaaaaaag tggcagcatt agagaaagat gcgcctcgct tacgttcaaa
    16621 agagattcaa gcacgtcaaa atgcgattca aaaagaagca aacgcattcc aagctaaagt
    16681 aacagcaatt caaaaacgtg aagctgattt tgcaaaaaaa gcagaagcct tccagaaacg
    16741 tgcagatgaa ttccaaaaac gtattgaaca agcaaataaa gaaaatggtg gcattaaccc
    16801 acaagaagcg caaaaacaag cggttgatga agtaaatgca accattaaag agatcgcaaa
    16861 atcgaaaggt tacactttag tattacaacc acaagtagca ctttatgctg aagatgaagg
    16921 taaagatatt actgaagcag tacttgatgc tattgttaaa aaacatccgg aaattaaaat
    16981 agaaaaacct gcaactgaag ctcaaccagc tacagaaaaa gaaaccaaag ctgaagaaac
    17041 taaaccagaa gaagcgaaaa aataatggca aatttccgtt taattgattt agcggagcat
    17101 atcggcggta ctcttagggg taacgccgat ttggctattc aaagtattgc tgctttagat
    17161 aaagccgaca gctcgcaaat tacctttatc tcaaatgcaa aatatcggga aaatttgacc
    17221 gcttgcaatg ctggagctat tatcgtcagc ccggcagatg tagaattttg ccgagcagat
    17281 caaaatctta tcatcgttgc caatccgtat gttgcttatg ctcaactggc tcaatatatg
    17341 gattctactc cgaaagcagc gagcgaaatc cacccaagtg cggtgatttc acctgaagct
    17401 aaactgggta ataacgtatc ggtaggggca aatgccgtga ttgaaagtgg cgtagaattg
    17461 ggcgatgatg ccgtgatcgg tgcaggctgt tttgtgggaa aaaacagtaa aatcggggca
    17521 agaacccaac tttgggcaaa tgtttcggtg taccacaatg tacaaatcgg agctgattgc
    17581 ttgattcaat cctcaacggt tatcgggagt gatgggtttg gctatgcgaa tgacaaaggg
    17641 caatggatta aaatcccaca aaccggtggt gtgattatcg gtaatcgtgt tgaaattggt
    17701 gcttgcactt gtattgaccg aggtgcatta gatccaaccg taatcgaaga taatgtgatt
    17761 attgataacc tttgccaaat cgcccataat gtacacattg gttttggcac tgccgttgcc
    17821 ggcggcgtca ttatggcagg cagtctaaaa gttggtcact tttgccaaat tggtggagca
    17881 agtgttatca acggtcatat ggaaatctgt gatggtgcca taattaccgg aatgggtatg
    17941 gttatgcgcc caattacaga aaaaggaatt tactcatcag gtattccgct acaaaccaat
    18001 aaagagtggc gtaaaactgc cgcattagtg atgaatattg atgaaatgaa taaacgttta
    18061 aaatcacttg aaaaacgttt taacgattaa ttttattaga ggtcttaaag tgacagaaat
    18121 gacaactgaa aatcgtgagc caaatattat tgaagtaacc gaaattatgg atatgttacc
    18181 gcatcgctac cctttcctgt tagttgatcg cgtaaccgat tatgaagaag ggaaatggtt
    18241 aaaagccatt aaaaatgtaa ccttcaatga accttgtttt accgggcatt ttcctggcgc
    18301 accaatcttc ccaggagtat tgattcttga agcaatggct caagcaactg gcgtattagc
    18361 cgttgccact tatggtaagt taagtgaaga cgaactctac tattttgccg caattgacaa
    18421 tgctcgcttt aagcgaccgg ttgtgccggg cgatcaactt caacttgagg ttgaatttct
    18481 taaagaagta cgtggtatta ctaaatttac cggtaaagca tacgttgatg gcaaattagt
    18541 ctgtgaagca gatttaatgt gcgctcgccg taaataattc gatataagcc caaactagag
    18601 ggaacaaact atgcgtctaa ttgattcaac agcaaaaatt agcccgcttg ccatcattga
    18661 agaaggtgct gaaattggcg ctcacgttga aattggtcca ttttcagtca tcggtaaaga
    18721 tgtcaaaatc ggggctagaa cgaaaattca ctcaaatgtt gtcattaacg gcatgactga
    18781 aattggtgaa gataaccata ttttccaatt tgccagcatt ggggaaatca accaagatct
    18841 aaaataccaa ggtgaaccaa ccaaagtggt gatcggtaat cgtaaccgta ttcgtgaaag
    18901 tgttactatt caccgaggta cggtacaagg cggtggcgtg acgaaaatcg gtgatgataa
    18961 cttatttatg atcaacaccc acatcgcaca cgactgctct atcggtaacc gttgtattat
    19021 cgccaataat ggtacgctag caggtcacgt taccctagat gatttcgtga ttgtgggagg
    19081 aatgtccgcc attcaccaat tcgttgtgat tggttcacac gttatgcttg gaggcggttc
    19141 aatggtcagc caagacgtac cgccttatgt catggcacaa ggtaaccacg cacgcccatt
    19201 tggtattaac tttgaagggt taaaacgtcg tggctttgat aaaccaacta tgcacgctat
    19261 tcgcaaagtc tataaactga tttactccag cggaaaaaca ttggaagagt gcctagtaga
    19321 aattgagcaa attgcagcaa cagaacctgc gattgctatt tttaaacaat tcttcaaacg
    19381 ttctacccgt ggtattattc gttaataaac gaaaccccga aatcaattcg gggtttgttt
    19441 tatcaagcga ccgtcgaacc tttcaacaat tttcgaatcc aagctcgatt tgggctaagg
    19501 ttatgccaaa tatcctcact gattggcaca ggctcattca caatcaggct tgctaacaat
    19561 tcccctagaa gtggtgcagt cgtcaaacct cgagaagaca ggccgtttac catatacaga
    19621 ttggtaaaaa tttcagcatt ttctaccgct tgtttccgta tcaactggtt gtataaattg
    19681 gcatattggc ttttttgctg ctcaaaatga tgcactgccc caaccatcgg tacacgatca
    19741 cgaaacgccg cacgaatacc gactttggct ttattttcag ataaatcaat tccttgcgtc
    19801 cactcacaaa cggttacatt ttgctgaagt tttgcaacat tctcccaatg ctccgataaa
    19861 ctaaactcgg tttctgcatt atcccgaata tggcttgctc caatgcaatg cgtgccgtag
    19921 gctgacattg gcgttaaata accatcataa caaagcacac acttcagttt ttgtaattcg
    19981 cttgtagtcg gaatttggct cacttgccct cgtaccggat acaacggaat gccgttcgct
    20041 tggctaaatt cattcaacgt atgtccattt gccagcacaa ccgcttgatg tcgaaccgtt
    20101 tctcccctcg tctgccattg ccacattcca ttagaaaagg ttaaatgctc aactttgtga
    20161 ttcagtacaa tcttcaaccc tttctgctcc agccaagtaa agccatttct aacaagttgt
    20221 accggtgaaa gccaaccagc ttgcggaata aatgccccgc tgtttggcac gcttaaacct
    20281 aatttttcac ttaattctgc ctgtgaacaa gattttaata aggtgtcatc agccgtctgc
    20341 tccaccattt tttccagttt tttagccgtt ttctcgttat aggcgtaaag cactaccccg
    20401 cttaaattat gctcaaactc aatgttggat ttcagttgtt gcaagcgttg caatgcataa
    20461 tcaaaactat ggatataaaa ctgtacattt cgctcgtcat catcgcttaa ctgtgggtaa
    20521 atcgcccctt gtaaatttcc tgaagcattt attgccaagg tttcatcttt gcaatacagc
    20581 gtgacttgct tgcctttttc taacagcgat aacgcaacga agaaactcgc tactccgcca
    20641 ccaataattg caatgtcatc agcggtttca tttttggcat aaaaataggg gtaattatgc
    20701 tcggaaactt ggctatctaa cggtttctcg ccccaaagca tttctcgttt tttgccaaat
    20761 cctttgcgtt ttttcactgc aaaacctgcg gcttccagcc ctcgtctgac catacttgaa
    20821 gcggtaaaag tagcaaaact gccgccattg gcacttagtc gaaacatttg cagatataac
    20881 tgttcgttcc acatttctag gtttttatca ggcgaaaagc catctaaaaa ccaagcgtca
    20941 atcgtgccgc tataaagatc gcctaattgg ggtaagtttt cagcaatctc accgaaccat
    21001 aaatctaaat aaacctgctc aaaatgataa cgttggcaac ctatttggcg aggctgccaa
    21061 caagcggtta aattttcaga taatgttgca aattgcggat agcgttggtg aatatcagct
    21121 aattggctgg ccgtcagagg aaatttctca aatgagataa aatagagtcg ttttagttta
    21181 ctttgcggaa atgctgcccg aaactgttgg aatttgtcag caacggctaa aaaattcaag
    21241 cccgtgccaa agccggtttc cgcaataaca aaagcctctt tcgggtgaga ttgccacttt
    21301 tcccacagtt ggttaccttc ttgaaacaca tagtaacttt ccgccaaacc atcttgggta
    21361 gaaaaataaa tatcatcaaa ttgctccgac accggtgtat ctgcatcatt aaatgtaagt
    21421 gtcgcaaaag tcagtttacc catcatatcc ccttaaaaac aaaacacgga aaaccccaag
    21481 attttccgtg tttcaaccag ttcaatagta ccaatattag aaaatgttgc tggcaaaaat
    21541 aacgatcata ccaagtgcgc ctacaaccgt tagcattgaa taaccaaaag tacccatttt
    21601 acaatcctca ttgttaatta tgattaaaac ggctttattc tagcataatt tccatcgctt
    21661 tgaatgccta tgcttgaata attttgccaa aacaagcggt caatttttgt aaatctttta
    21721 cgcctttggc actttccacg ccagaattta aatctaaccc gacacagcct tgagcaaggg
    21781 cttgctcaat attctcagtg ccaatgccac ctgccaataa cgctttttgt tttaaggttt
    21841 ctggaatcaa cgcccaatta aaggttttgc ctgtgccgcc ttgctgattg cctactttgc
    21901 tgtctaatac ataacgagca atctgtggat tatcgttaaa ttctagattt tcaacctcaa
    21961 catcaaccga taaggctttc cagatttgag ttttactacc taatttctct gccagttctg
    22021 caatgtattg ctcatcttcc gagccgtgca attgaactgc aaataactca agctgttttg
    22081 ccattttttc cacaaattcg accgcttgat tttggaacac ccccacataa cgcaacggtg
    22141 cattcaccac caattcttgg gcttggcgaa gtgaaagctg gcgaggcgag ccttcggcaa
    22201 aaattaaacc gccatataat gccccttgct cataaaccgc tttcacatct tgcacacggg
    22261 ttaagccaca cactttgttt tcaccaaaaa tcaccgcccg caccgcatta ttgagatcgg
    22321 cacttcccat taaactgctg ccgattaaaa aagcatgaac atacggctta atgtttttca
    22381 cttgctggtg atcgttaata cccgattctg aaatcaaaca gacatcagcc ggaatttgag
    22441 cttggtattt ttccaccaaa cgcacaatgc ggttcatatc aatcgtgagc gtgtgcaaat
    22501 cacggttatt aacgccaatt accttcgccc ctaaggctaa ggcacgttca aattccgcct
    22561 ccgtgctggt ttcagtcaaa acgcccattc ctaactcgtg agccagtgtg gcaagttcac
    22621 ggtaggtttc atcatttacc accgataaca tcaataaaat cgcatcagca ttataaaaac
    22681 gggcaaggta aacctggtat gccgaaatca taaaatcttt acacaaaatc ggctgtgtgg
    22741 tttggcgttt gacttgatcg atataacgga aatcgccttg aaaatattgc tcatcggtta
    22801 aaaccgaaat cgccgcagcg taatttttat acacttgggc aatactgtct aaatcgaact
    22861 cagcacgaat taaccctttt gacggtgaag cttttttaca ttctaaaata taagctggtt
    22921 cttggtgtgt gcctttggca agagccgcat agaaatcatg gtcgcttttt acaattttgt
    22981 gctgaaattc acttaacgga aaggcttgct ctttttgtgc cacccaaatg gctttatctt
    23041 gtacgatctt ttgtaaaatc gtcggttggt ttggttgagc gttatcttga acgatgtttt
    23101 gcatataaat tcctagagcg aaatcggttt gaatggtaaa atctgccctt atttttaccc
    23161 gaaaaagtct catttcacaa gcaaaactaa aatgaaatca caagttatac agcatttatc
    23221 ttatttagaa atcgccctga gagttaatca actttgggag gcgacaccac caagccctga
    23281 agccttagcc aatgagcaac ctttctgcgt ggaaacctta accccaaccc aatggctaca
    23341 atggattttt attcccagaa tgcgggccat tttagatgca aatgccgatt tacctcgcaa
    23401 ttttgcaatc accccttatt tagaagaggc attgaaaaac gaaggttatt tacaagcgat
    23461 ttgtgaaccg ctattaggtt tggaaaagtt gttgaaagat taacttctct ctcagttctt
    23521 ttattaatct gacaaattcg ttgatagtgc cagcgtccac gctggtacta attttaacgg
    23581 ttaattaatc aaaatttggc acaagcgagg acgcttgcgc catcatagag atttgataaa
    23641 ttatacttaa tgtattatga ccttagatat tttataccaa gacaactcgc ttatcgccat
    23701 caacaaacct gccggagtgt tggtgcatcg ttcttggtta gataaacacg aaacggtgtt
    23761 tgcaatgcaa accctgaggg atcaaatcgg gcaacacgtt tttccgattc accgccttga
    23821 ccgcccaacc tcaggtgtat tgctgtttgc tctcaacagc gaaaccgccc gaataatgag
    23881 cgagcagttt gaacaacacc tagtacacct agtacaaaaa acttatttgg cggtggtgcg
    23941 tggctattta caaggttcgg cacaaattga ttatccgctc aaagtgattt tagataaaat
    24001 tgcggataaa ttttctcagg aaaaagaggc tcaagcggcg attactgatt ataaaggctt
    24061 agcaacggtt gaaatgcctt accctgtcgg caagcaccaa acctctcgtt atagtttggt
    24121 tgaacttagc cctaaaaccg ggcggaaaca tcagcttcgc cgccacctga aacacctgtt
    24181 ccacccgatt atgggagaca gcaaatatgg cgacttacac caaaaccgtg ctttagttga
    24241 aaaatcaggc gtaagtcgtt taatgttaca cgcccataaa ttgcaatttc agcacccgaa
    24301 aaatttgcaa aaaatagaga taattgcatc gcttgatgag caatggcaac gcctctttgc
    24361 attttttgac tggaacttta ctcaatatta ttagacaaaa aagaggattg aatggataat
    24421 tcacttttat cattgacaca cgagcaacaa caagccgcag tagaagaaat tcaaaaactg
    24481 atggcacaag gcatcagtgc cggagaggca attcaaatta ttgctaatca attacgtgac
    24541 aaatatcaaa tattggagaa atcaaacaat gaataaatta tggacagtgc ttgccctaac
    24601 aataacttcc cactatagct ttgctgccgc tgaaaaaata gtcactaaag atttaccggc
    24661 agaaggaaaa cctgcagtcg tgctacaggt ttttgatgaa agtgaaaaaa ttgcaaaacc
    24721 tattaaggga aataagctct ttattagcaa aaaacaaaca aggctttgct ggcacagtat
    24781 taatcttcct gtacaaaata aagttactat tgcagaagct ttctatgccc ctgctgcaat
    24841 gaaattagct tctcccggct ctaaaattaa ttcttctgca gacaagaaaa atcatactat
    24901 cattactgaa ataaaccttg caaataatca agtattaaat cgttgctggg gttttgataa
    24961 aactgaccct gtcggtaaat acaaaatgga aattcaaatt aacgatcaca ttttcaaagg
    25021 tttagaattt gaaattgtaa aataacctaa aagccacaga gtaacaccct gtggcttttt
    25081 gctaaatcat tgtttttaaa ttggttaaat tgtgcagcaa ataatcttta ctcttcacaa
    25141 aggctctcgc ctgttgccag ttgctaaatt gttgctgagt gcattgatta aaacgtacca
    25201 gcacttccaa gctttcattg ccgacacaaa taaaatcagc taataattgg gttaattttt
    25261 cactgttttc accatccatt gcagttgcaa ttttctgctc ccatttaacc gcttgtccga
    25321 gcaaataacc ccgctgggct ttgcttaaat tactcaacca catcccatcc gcttttggca
    25381 ttgcctcaac caaagtaaat aagtcgccta gatttccttg cttgatttca aactccaatt
    25441 cacaaatcgg ctcttcacca aaatgatttt taatgatgcc ctgatctaac gctacctcaa
    25501 tgtcagacat ccccacttgc accaaccaag ttttacgagt gaaatcggtg ctgaatatcg
    25561 cttgcaagct ttctgcaatc tgctctgaat tttcaaacgg caactggtat ttttccacta
    25621 atgtttgaaa atccggctgg ctattgggta aagctaaatt atattccgga cgaatatgca
    25681 aaccgcctac aatttcccct tgtgttttta acgtgatttc atattgttga ttcacagaac
    25741 gcacccgcag ccccattttt tgcgtggcaa aaaattgctc gggcgtatca taataggtgt
    25801 tgcctaacaa ttccgtttga tgagcaagca cagggaatgg cgaggtttga ttcaagtagt
    25861 tttccactaa cgccacattc tgctgttcca acattatttt cagttcgatt tctgcttgtt
    25921 tttggttttc catttcacac gcccctgcaa cgttgtataa tcaagcggtt attttatcaa
    25981 taaattttgc aaaatgtttt caaaaattca ccataaactg aataactttg tccagcgtgg
    26041 actcgacaac catttacgcc tcgccgttac cggcttaagc cgtagtggca aaacggcatt
    26101 tattaccagc ttggttgatc agcttttgca tatcaacaaa ggcggtaatt cgcatttaaa
    26161 cctgtttgcg cctgcacgcc acgggcaaat tctatcggta aaacgcgttg agcaagggga
    26221 tttaactatt ccccgctttg aatatgataa aaaccgccaa agccttgaaa atcaaccacc
    26281 tcaatggcct gaatccacca ctggcattag cgaaattcgc cttgcgattc gctaccagcg
    26341 gcaacattct ctgctacgtc atattaaaga aaccggcacg ctttatttgg atattttcga
    26401 ttacccgggc gaatggctgt tagatttacc cctgctctcc ctttcctata aagaatggtc
    26461 gcaaacgcaa caaaccatac ataaagatga gcgtgcagag cttgccacac cttggttaca
    26521 agcggttaaa aaactcgatc tttttgcaaa aaccgatgaa aatttactgg cagaattcag
    26581 ccaacaatac accgattatt tgctcgcctg caaagctcaa ggaatgcaat atattcagcc
    26641 ggggcgattt gtgttgccaa ctgaaaatgc caaaggtgca ccggtattcc aattttttcc
    26701 gttattagat ttaagcgaag ctgagtggga aaagttggaa caaagcgaca gaaacagtat
    26761 tttccatacc ctcaaaaaac gctatgagca ttaccagaaa aaaatcgtca aaccgtttta
    26821 tgaagattac ttctctcaat ttgaccgcca agtgattttg gcagactgcc tcacaccgtt
    26881 aaattatggc tataacgaat ttatggaaat gaaattaggg ttacagcaac tgtttaaaca
    26941 tttccattat ggcaatcgtt cgcta
//
LOCUS       DANKBV010000018        26714 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.018, whole genome shotgun sequence.
ACCESSION   DANKBV010000018 DANKBV010000000
VERSION     DANKBV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 26714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26714
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.018"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(193..1068)
                     /gene="ubiA"
                     /locus_tag="PWY43_000607"
     CDS             complement(193..1068)
                     /gene="ubiA"
                     /locus_tag="PWY43_000607"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDL2135512.1"
                     /translation="MNNETLFSKQKWIAYAQLMRFDKPIGTLLLLHPTLWALFIAAEG
                     MPPVAILAIFTLGVIVMRAAGCVINDYADREIDGHVKRTSQRPLATGRVTTTEAKVIF
                     AILLLFAFLLDLMLNRYAFLLSFVAVFLAVIYPFMKRFTHLPQVVLGMAFGWSIPMAF
                     GAVTETLPLECWLLFFANLAWTVAYDTQYAMVDRDDDLRIGVKSTAILFAQYDNKIIA
                     LLQFITLILLGLLGVVKDYHIGYFVVLALSATLFIYQCWLTKKRERSECFKAFLNNHY
                     FGLGVFVAILVGIYF"
     gene            complement(1061..1822)
                     /locus_tag="PWY43_000608"
     CDS             complement(1061..1822)
                     /locus_tag="PWY43_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDL2135513.1"
                     /translation="MKQANRHKKIIELVNQLGYVSTEQLVAELNVSPQTIRRDLNEMA
                     ENNLIRRHHGGAAAPSNTENSDYTHRKQFFSHEKNAIAQQVAKLIPNGASLFLDIGTT
                     SEAVALALLSHKNLKVVTNNLNAAHILMQNEDCQITVAGGGLRSDGGLIGEETVRFIN
                     QFRLDFGILGISAVDMDGSMLDYDYHEVQVKRALMECSRQVVLVTDHSKFTRTAIARL
                     GNVKEVNYLFTDQDLPLELQTHLSQSDVIVKICNE"
     gene            complement(1889..3256)
                     /gene="purB"
                     /locus_tag="PWY43_000609"
     CDS             complement(1889..3256)
                     /gene="purB"
                     /locus_tag="PWY43_000609"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL2135514.1"
                     /translation="MELTALTALSPIDGRYQDKVASLRPIFSEFGLLKFRVMVEVRWL
                     QKLASHTQINEVPVFSEKANDYLNQIVADFSLEDANRIKTIERTTNHDVKAVEYFLKE
                     KCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKVIDAVAELAKC
                     YQHIPLLSRTHGQPASPTTMGKEMANVAYRLQRQYKQLENLEILAKINGAVGNYNAHL
                     SAYPEIDWHTFSQEFVESLGVTWNPYTTQIEPHDYIAEFFDCVARFNTILIDFDRDMW
                     GYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAVMAHLGQKLPISRWQ
                     RDLTDSTVLRNLGVGLGYALIAYASTLKGISKLEVNEQHLRDELNQNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDETAMREFIDKLEIPENEKNRLKEMTPASYIGYAVE
                     LVDKL"
     gene            complement(3290..3919)
                     /gene="hflD"
                     /locus_tag="PWY43_000610"
     CDS             complement(3290..3919)
                     /gene="hflD"
                     /locus_tag="PWY43_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDL2135515.1"
                     /translation="MADYHDITLAYAGVCQAATLVQQFAHKGMADREAFKCALESLLI
                     TQPESTLSVFGDDLSHLKMGLETALAQTGGGNGKLDTEVGRYWISLLALSQKLNKNPE
                     AKQQLAQRLQQVERQLALYEDNMMADQMIANLAAIYSDIISPLGTKIHVMGMQDYLVR
                     PDIQNKIRASLLAGIRAGILWQQVGGSRWQFLFSRKKIFNQAQKFYRQI"
     gene            complement(4027..5715)
                     /gene="cydC"
                     /locus_tag="PWY43_000611"
     CDS             complement(4027..5715)
                     /gene="cydC"
                     /locus_tag="PWY43_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDL2135516.1"
                     /translation="MKSLFPFFALYQTHFGRLLLGVVLAILGLAASIGLLSLSGWFLA
                     ASFLAGNALIFNFFYPSSGVRGLAVGRTISRYFERLVTHDATFRVLANLRASVFKKLI
                     PLSPSGLNRFRNSELLNRLVADVDTLDTLYLNLVSPFVSAVMIIAFMAIGLSFVSVPL
                     MLIICGTLLVLLAVIPTVFYRLGLKLGRNAIQSRANYRSQFIEWVQLNAEFLLFGNLN
                     QMSEKLQQTEHQWLNAQSKESRLSGLSNSLIMLSNGILTCVVIYLVSTSINVPTAKYP
                     EALIALVIFCVMASAEILSPIGIAFLHLGQVITAAERISEITEQQPNVTFGSKAEWQN
                     LSQNQPLVRFENVSFAYYGEQNVLNNLSFEVLKGQKVAILGKTGSGKSTIFQLLNRNY
                     DPTSGQIWLNNCKIDEYSEPMLRSKLVTLSQRVHIFSQTLKDNLLMGNAQATDEQMQE
                     VVRKVGLGHLLEIDGLNLWLGEGGRPLSGGEQRRLGLARLLLSSAELVLLDEPTEGLD
                     RETEQQIINLILTHCCDRTLIMITHRQSGLDKFDVVYRIDNGRLVGWFLQQIAY"
     gene            complement(5715..7481)
                     /gene="cydD"
                     /locus_tag="PWY43_000612"
     CDS             complement(5715..7481)
                     /gene="cydD"
                     /locus_tag="PWY43_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDL2135517.1"
                     /translation="MEKQRQRELQKWLKSQQKVIKKYMHLNILLGSFSSLCMIGQMWL
                     IATMLDKMLIGKQSPNSFLVEIMLLFCCFAARAFFIFLRERVGFKAGQTLRLHLRRQI
                     LAKMEAVGPMSIQQKPAGSWATLMLEQVENLHNFYARYLPQQFLSLIVPLLILCFVFP
                     INWAAGAILFATMPLLPLFMILAGMKAVEANQRNIGILSRISGQFLDKLKGLETIRLF
                     GQAEKQTEQIYQSTEDFRISTMDVLKMAFLSSAVLEFFTAVSIAVTAVYFGFIFLGEL
                     DFGYYGTGVTLFIGFFCLMMAPEFYQPMRELGVFYHDKAAAISAADNIETFLKEKVKT
                     QGGNLQKNVENQPLVIQAKDCVIFSPQGKALTQALNFELNAHQHIALVGQSGAGKSSL
                     MNMLLGFLPYEGSVTINGTELRDLNLSQWRAKLAWVGQNPQLMHGSLKENILIGNPNA
                     TDSELAKALQLSKADEFVARLGLEHQVQDSNIGISGGQAQRIAIARALLRPYELLLLD
                     EPTASLDMDSEQQVLAALHNLSREQTTLMITHRVEDLTQCDEIWVMKQGQIIQKGRFT
                     ELEHTGFFAELLHNELVHQGEI"
     gene            complement(7596..11696)
                     /locus_tag="PWY43_000613"
     CDS             complement(7596..11696)
                     /locus_tag="PWY43_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HDL2135518.1"
                     /translation="MTDENKQTATNQTDSAQEPQATQPKKSKWRFLKHGFCLTFILLL
                     LAILFLCTGYGQRKVLSWVDKFVEPLQLGQVQGSLQDGLQLSDARFVMDGVNVTVGQA
                     DLHIGFSCLLHYEMCLNNLSLKDTEVLIDTTKLPPSQPKEETEPFTELNLPLGISAQN
                     INLENIKVKVDDMDIHLNHFHTALSGKGRAITVHPTQLEGLNLLLALKLDETDTEQAV
                     DFAQKNAKTEALSATNPQEIADKVIQEAVVEKTSEKVVEGFHQLQRKTVAQAEQVDNK
                     VDWAAIKAQLAQPLLDKNIRFSLPLDLNIEQIDIANVAIAQKAKDEKGNLIEPISLLN
                     VASLQLQAQAKDQHINLKSLDFKSDQGDLTASGSLSLQDNYPLSWTLSGHEAKTAKVK
                     LPFNQIEASLSGELYDKTSLKVKTAGAISASLEGNVALAQAKTPFDLSLKSESMRYPF
                     APQQNGDVLSLENIAMRLNGNLLDYALEAKVSAKGMGVPASSASLTGKGELTHFNIQD
                     LSLNTLEGTAKIDGLVDWSEGVAWRSNLKLQHINTKSLLADWPAVLSGSLSSEGYAGR
                     GNSASGWKADVSNIDIKGSLLQKNLHLSGNLKADHQQLLDVPGLSLVYGENKIDLKGV
                     LGEKSDFYADIKAPNLQGLVPNLKASVNGNAKLSGKVSEPNIDLDLVASNVGYEQFKL
                     QNLTAKGKITTEKTIQGDLTLGLRQFAYNEIKVENATLLLKGNEENHSLKLSAKGNPV
                     GADLQISGKFDRLKEIWEGQLSQVSIQSTEFGQFQADKAVNVKYDNKAINANISAHCW
                     HNRKINLCFPTAFNAGVEGKVPFEIRNFDLAVLQQFLEKNSQLSGIVNAKGDAAWFKN
                     KQPQVNLDLTSNAIKFIQKMEGGKSFPLTVSPLKIDLKMSDNNLTLKSDLRVENNGRL
                     STDLVMKDLSKARALSGNIHLDQLSLKLIRPLLERGDSVDGNVNARLTVSGTATSPLL
                     HGNLNVTELKARSVTMPFDITGGNLAMNFHGATSTLSGKLQTKQSELRLEGDADWRDL
                     NAWKTRVHAQANRFQVDIPNIAKVAVSPDIQVTATPTLLTLSGNVDIPWARIEVEQLP
                     ESAVSVSGDEVIMDGSVKNKVPFSQRNIPAQTSGGMAINADIKINIGNDVKLKAYGLN
                     SNVNGLLSVRQGKQGLGLYGQVHLREGRFAAYGQDLLIRKGDISFAGSPSQPTLDIEA
                     IRNPEAMEDPTITAGVRVTGLADNPTVKVFSDPAMSQNEALSYILTGRSLESSGDTSS
                     SNAVAAALLSMSLSKSSKLVGDVGSTFGLKDLSVTTAGIGDNTKVEVSASLAPKFRVK
                     YGVGIFAPLTELTLRYNLTPRLYLQWVSSINQAVDLMYRFEFDELF"
     gene            complement(11777..13612)
                     /locus_tag="PWY43_000614"
     CDS             complement(11777..13612)
                     /locus_tag="PWY43_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HDL2135519.1"
                     /translation="MNTKLHFLTFCCLVAFSSMAQANQEQRLSDVDRERVENLQEKLA
                     EDSDDEPEIEPLVTLEVKGIQDKDAKKNVELYLDQIANEYADGSERYQYLVQTTVDKA
                     LRALGYYNSRYQFVQLPQEGKKPLLVLNVELDKETVKIDETDIVLQGDAKTDEDFAHL
                     VSKAPKQGSRLNHKTYDNFKGDIESTAFSKGYFDGHWLYHRLEVYPTDHKADWRLGYD
                     SGKRYHYGQIRFVDSQIKEEYLQNILKIHSGEPYLANDLSILTQDYLSSNWFSSVMVE
                     PHLNEQDKLVDLNVLFQPKKRNEVEIGIGYETEVGPRLQLNWKKPWLNSRGHSIESRT
                     YVSKPEQTFEFGYNIPLREDPLHYYYQLSGTLENEDQNDTKSTAATLGLQRFWTHETG
                     WSFSVGLKARYDSFTQANDRFKTLLVYPTASLNRTRTDGKPYPLWGDTQKLTVNWGSR
                     AVGSDVNFYSYKASTSWARTYFDNHRFYLRAELGYLKANQFERIPPALRYFAGGDMSV
                     RGFGYKDISPRDPSNGKRVGGSHLATGTVEYQYQVYPNWWGAIFYDAGLASNKFKTQD
                     LYYGTGLGVRWASPIGAIKFDVATPVKSPDNSKGIQIYIGLGSDL"
     gene            complement(13624..14469)
                     /locus_tag="PWY43_000615"
                     /pseudo
     CDS             complement(13624..14469)
                     /locus_tag="PWY43_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248088.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
     gene            complement(14470..15633)
                     /locus_tag="PWY43_000616"
     CDS             complement(14470..15633)
                     /locus_tag="PWY43_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2135520.1"
                     /translation="MLVKRINSYCWTAIFSGVISFSTPFSFANNLNQIQQKIQQQTSK
                     INEQKQKRNALQSTLKNQEVEMGKVLDKLQKTEMSLTEVRQTIKATETEIARLEKLEK
                     QQKEKLKEQLDSAYRSGVNPSVLERLMSEEAKNAERMTAYYQHMNNIRIDAIVEIRKT
                     QAALKARRDELKGQQKDHQTQLNEQKKQEKDLKKVQSQRESTLRLIDKTLEQDQSRLE
                     NLRSNEQALKQQLEQAQREAQQAEKREQQREKQAKSGQSKTTENVERTPIKAGRYTMP
                     VSGNIITPFGNNWHGVVIGASAGTSVRAMASGRVIMAQWLAGYGNMVAIDHGNGDISL
                     YGYNQSISVSKGSRVQGGQVIAKVGNSGGQSRSALYFGVTRKGKPINPLNLVK"
     gene            15801..16484
                     /locus_tag="PWY43_000617"
     CDS             15801..16484
                     /locus_tag="PWY43_000617"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2135521.1"
                     /translation="MQLVFIRHGFSEWNAKNLFTGWRDVNLTERGIEEAKSAGQKLKA
                     AGFEFDIAFTSVLTRAIKTCNIVLEESDQLWIPQVKNWRLNERHYGALQGLDKKETAE
                     KYGDEQVHIWRRSYDISPPDLDPQDPNSAHNDRRYANLPKDLIPNAENLKITLERVLP
                     FWEDHIAPALISGKRVLVTAHGNSLRALAKHIEGIFDADIMDLEIPTGQPLVYELDDN
                     LKVISKRYL"
     gene            complement(16621..17178)
                     /gene="frr"
                     /locus_tag="PWY43_000618"
     CDS             complement(16621..17178)
                     /gene="frr"
                     /locus_tag="PWY43_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL2135522.1"
                     /translation="MINEIKKDTQDRMEKSLEALKGHIAKIRTGRAQPSLLDGIQVEY
                     YGSATPLRQVANVVAEDARTLAVNVFDKSLISAVEKAILTSDLGLNPSSAGTTIRVPL
                     PPLTEERRRDLIKIVKAEGEQGKVAIRNVRRDANDQIKALLKDKEISENEQHKAEEDI
                     QKITDTFIKKVDEVLAAKEKELLDF"
     gene            complement(17230..17946)
                     /gene="pyrH"
                     /locus_tag="PWY43_000619"
     CDS             complement(17230..17946)
                     /gene="pyrH"
                     /locus_tag="PWY43_000619"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL2135523.1"
                     /translation="MSKPIYKRILLKLSGEALQGSEGFGIDPSILDRMALEIKELLAE
                     GVEVGVVLGGGNLFRGAKLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAEVNAKLM
                     SAFQLNGICDTYNWSEAIKMLREKRVVIFSAGTGSPFFTTDSAACLRGIEIKADVVLK
                     ATKVDGVYDKDPAKYADAKLYNKLTYAQVIEQELQVMDLAAFTLARDHGMPIRVFNMG
                     KPGALRNVVFGTEEGTTISE"
     gene            18038..19600
                     /locus_tag="PWY43_000620"
     CDS             18038..19600
                     /locus_tag="PWY43_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL2135524.1"
                     /translation="MKSKQLFGILVAISLATAASCLMLIGSGFFPNPSIFEVILGVFL
                     IIALASSKWTYYLLLLPISIAYAIYTPVGIKFGKPTYEYIASIFATDMMESKEFIAQL
                     PILNIAAGIAIVLAVIFYRKITNRYQIRFLRNKTFVISAFVLMLFSLAPFKFFQEFYN
                     SSLKVKKELEVLNNLTLDSQWGQSTLSSTSNYDDYVLIMGESARKDYHHAYGYPAANT
                     PFMSSAKGTLIDGLTSGGTNTIASLRLMLTKPDTEKWEANYGLTLIDLIKSTGIKTYW
                     ISNQGYLGNYDTPISSIGNKSDVKIFTKAGDSLNTNVSDFELLPHFAKVIEEPAQGKR
                     FIFLHLYGSHPITCDRLTDYPKMFDDDKLPKRYFNVNCYVSSIKKTDELLKRVYDLLV
                     KNQENHQRSFSMVYLSDHGLSHEVSKENIVIHNGKDRSKLHYDIPLFKISSDDTQRNE
                     YKAFKSGLNFTNSIANWIGIENPLLDKEINLFNEQNDKSDYGLKKIIETQSTVEDPAV
                     PIPESYLYKATN"
     gene            complement(19665..20516)
                     /gene="tsf"
                     /locus_tag="PWY43_000621"
     CDS             complement(19665..20516)
                     /gene="tsf"
                     /locus_tag="PWY43_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL2135525.1"
                     /translation="MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKS
                     GQAKAAKKAGRVAAEGVILARVASGFGVLVEMNCETDFVAKDAGFLELANEVADFAVA
                     NKGTTIEALQAQFEEKRAALVAKIGENMNIRRVQYLEGEVIAQYLHGAKIGVLVAGAG
                     SEDELKKVAMHVAASKPEFVNPEDVSADVVAKEREIQIEIAMNSGKPKEIAEKMVEGR
                     MAKFTGEVSLTGQPFVMDPSQSVGQYLKSVNTSVSNFIRLEVGEGIEKVEEDFAAEVA
                     KITGGNA"
     gene            complement(20612..21331)
                     /gene="rpsB"
                     /locus_tag="PWY43_000622"
     CDS             complement(20612..21331)
                     /gene="rpsB"
                     /locus_tag="PWY43_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL2135526.1"
                     /translation="MAQVSMRDMLNAGVHYGHQTRYWNPKMKPFIFGARNGVHVINLE
                     KTLPLFNEALAELTRIASNNGKVLFVGTKRAASEAVKLAAVDSQQFYVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQTQDGTFDKITKKEALMRTRELEKLELSLGGIKDMNGLPDA
                     IFVIGADHEHIAIKEANNLGIPVFAIVDTNSTPDGVNYIIPGNDDATRAIQLYLDSAV
                     AAIKEGRGVETVEVAEEASAE"
     gene            21710..22738
                     /locus_tag="PWY43_000623"
     CDS             21710..22738
                     /locus_tag="PWY43_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2135527.1"
                     /translation="MKKTLSALAIAAATFTSTSTLAANEVNVYSYRQPYLIEPMLKNF
                     EKDTGIKVNIIFADKGLVDRVKQEGELSPADVLLTVDISRVMEIVNADLAQKIDSKVL
                     EKNIPAQFRDSNDQWFGLTTRARVIYTSKDRVGKLPAGFDYLDLAKPEYKGKVCVRSG
                     KNSYNVSLFAAMIEHYGIEKTKAFLEGLKANLAQKPQGGDRDQVKAIKEGICDYSIGN
                     SYYYGKMLDDEKQKSWAEAAIINFPSGEYGTHKNISGVVIAKHSPNKANAVKLIEYLS
                     GEKAQGLYAELNHEYPVKEGIEPSKLVKGWGTFTADTIKLEDIAKHYEAALKLVDEVK
                     FDDFSEKK"
     gene            22861..24507
                     /locus_tag="PWY43_000624"
     CDS             22861..24507
                     /locus_tag="PWY43_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2135528.1"
                     /translation="MRSQFCWKSIAALTTFIVLLPLIAICYHALQGDNENLAHLWQTM
                     LPIYLKNSTLLVLGTVFLALIFALPTAWIVANYRFWGQRTLQWLLCLPLAMPAYLIAY
                     LYTDLLDYSGAFQGWLRSIFGWTHPQDYWFPQIRTLYGACFVLALVLYPYIFLLVRVA
                     LLEQSENLTHSAKMLGASPSQLFRRITLPLIRPSVAVGIALVAMETLGDFGTVAYFAV
                     PTLTTAIYDSWLGYHDLGTASQIAIFMLLMIFLFLSLEQYSRRKQKSYQRGYEKKSAF
                     KFLTGWKLALALAWCWGLLALAFFIPFGRLLYWAYDYFEQAWNLDFVQFSLNSLKVSI
                     IASVITVLIALLLHFSKRLNTRKILVKNDRLSLQLASLGYAVPGTVLAIGLLSPLTFA
                     DHQLHAWLKSLELSPVGLIFSGSLFALVLAYTIRFLAMAIGSLETSLNKISPSLDMAS
                     QTLGKNGSAMLVKIHIPLIYKGILTAGLMVFIESMKELNASLLLRPFNFDTLATYVFT
                     FTSDEQLERAALPAIVLVLVGLLPVIWLTRSLISQSEKER"
     gene            24517..25527
                     /locus_tag="PWY43_000625"
     CDS             24517..25527
                     /locus_tag="PWY43_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2135529.1"
                     /translation="MSVLTINHLNLNFGSTKVLQNLHLAVNQNEIICLLGASGCGKTT
                     LLKAIAGLLPIEQGDIHLAGECLKSKAVEERQIGLIFQDYALFPHLTVADNIQFGLSK
                     LPKVQQKAITEKMLSVVHLQGFESRFPHELSGGQQQRVAIARALACQPELLLLDEPFS
                     NIDSQTRYAMIQEIKQILKSQNVPAIFVTHSKEEAFAFADKIAVMDQGKIIQFGKPNE
                     LYHSPVNPFVADFLGGTNYLNCNVTEDNVLCSPIGEFRLFPEMNVQKGRYRWLLRPEQ
                     IILKPNELGLGKVVSKLFLGLYYRYQVEIKGIEIVTYQSQELKIGEQVEVGFQVREFI
                     LF"
     gene            25537..26163
                     /locus_tag="PWY43_000626"
     CDS             25537..26163
                     /locus_tag="PWY43_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HDL2135530.1"
                     /translation="MKIEVWSDYACPFCYIGKRHLEQALAQFEGEVEVVFRAFELDPH
                     ANGEPEGDIQQRLMRKYQKTAEQADEMIRYVEQAGKQAGLDLRYRTTQYTRTFEAHRL
                     AKFAKSKGLGEVMIERLFKAYFSDNLILAKRTQLIDLALELGLDRDEVAQLLTGDDFG
                     HEVREDERMAHRYGINSVPFFVIDEKLGVSGAQPPKVLLDAIKQALQK"
     gene            complement(26225..>26714)
                     /locus_tag="PWY43_000627"
     CDS             complement(26225..>26714)
                     /locus_tag="PWY43_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HDL2135531.1"
                     /translation="KSAAETFRANKEVNVVEAITQLGVGQALVSVLDEKGMPTPVEIA
                     YIYPPKSQLAPLAMGERDAIVKQDELYHHYSQYVNNQSAFDLLNAKAEAIKAEQAAAE
                     EANNDFFGGILSSIFGSKKKKDQSITEQVVSQVAHSVGRNLRNQVTKQIMRGILGAIT
                     KK"
BASE COUNT         7665 a         5799 c         4991 g         8259 t
ORIGIN      
        1 tggcattgta atgtcccttt tttgaatgtc tactacaaaa tgggatattt tacaaaccga
       61 ttgactaatg aagcaagaat ataattaact acaactggat atattttaca aatttcccga
      121 taaatttcac cgcttgtaga taaaaaccat agataaaaaa taggagctaa acatttttag
      181 cccctaccgt catcagaaat aaatcccgac taaaatcgcc acgaacacac ctaagccgaa
      241 atagtggtta tttagaaacg ctttaaagca ttcgctacgt tctctttttt tggtcagcca
      301 acattggtag ataaacagcg tggcacttaa ggctaatacg acaaaatagc cgatgtgata
      361 atcttttacc acgcccaata atcccaataa aatcaaggtg ataaactgta ataacgcaat
      421 aattttatta tcgtattgag cgaataaaat tgcagtggat ttaactccaa ttcgtaaatc
      481 atcgtcacga tcaaccattg catattgggt gtcgtaggcg actgtccacg ctagattggc
      541 aaaaaacagc agccagcatt caagtggaag agtttccgtt accgcaccaa atgccatcgg
      601 aatcgaccaa ccgaatgcca ttccaagcac tacttgtggc aagtgggtaa aacgcttcat
      661 aaagggataa atcaccgcca gaaaaactgc cacaaaggaa agcagaaacg catagcggtt
      721 tagcattaaa tcgagcagaa aagcgaacaa cagcaagatg gcaaaaatga cttttgcctc
      781 agtggtggtc actctgccgg tagctaatgg gcgttgagag gtgcgtttaa cgtgtccgtc
      841 aatttctcta tcggcataat cattaatcac acaacctgcc gcacgcataa cgattacccc
      901 aagggtaaaa atggctaaaa tcgcaacagg cggcattccc tctgcggcaa taaataacgc
      961 ccatagtgtg gggtgtaata ataataacgt gccgatgggt ttatcgaaac gcattaactg
     1021 ggcgtaggca atccattttt gtttagaaaa aagagtttcg ttattcatta caaattttga
     1081 cgataacgtc cgattggctt aaatgagttt gtaattctaa cggtaaatct tgatctgtaa
     1141 acaaataatt tacttcttta acatttccta aacgtgcaat agctgtacgg gtaaactttg
     1201 aatgatcggt cactaaaacc acttgtcggg aacattccat taatgcacgc tttacctgca
     1261 cttcgtggta atcgtaatcg agcatagagc cgtccatatc caccgcacta atgcctaaaa
     1321 tcccaaagtc caaacggaat tggttaataa agcgaaccgt ttcttctccg attagcccgc
     1381 catcacttct tagccctccg cctgctaccg taatttggca atcttcattt tgcatcagaa
     1441 tatgagccgc atttaaatta ttggtaacta cttttaaatt tttatgcgaa agtaaggcta
     1501 atgccactgc ttctgaagtc gtgccaatat ctaaaaataa tgatgctcca tttggaatca
     1561 gttttgccac ctgttgggca atcgcatttt tctcgtgtga gaaaaattgc ttgcggtggg
     1621 tgtaatcact attctccgta ttggaaggag cggctgcccc accgtgatga cggcgaatca
     1681 gattattttc tgccatttca ttcagatcac ggcgaatggt ttgcggactc acattcaatt
     1741 cagccactaa ttgctcggta ctcacatagc caagctgatt aaccagctcg atgatctttt
     1801 tgtgtctgtt tgcctgtttc atatttcctc ttttgcaaat aatttgcaga aaaagaccgc
     1861 ttgcaagcgg tcttttttca gttaaatttt acaatttatc aacaagttca accgcatagc
     1921 cgatatagct tgccggtgtc atctctttca aacgattttt ttcattttcc ggaatttcca
     1981 acttatcgat aaactcacgc attgcggttt catcaacccg tttgcctcga gtcagctctt
     2041 taagtttttc atacggtttt tcaatgccat aacgacgcat aacagtttga attggctctg
     2101 ccaatacttc ccagttttgg ttgagttcat cacgcaagtg ttgttcattc acttctaatt
     2161 tgctaatacc ttttaaggtg gacgcataag caattaacgc ataacctaaa cctacaccta
     2221 aattacgcaa cacggtggag tcagttaagt cacgctgcca gcgagaaatc ggcaattttt
     2281 gccccaaatg agccattacc gcatttgcca agccaagatt gccttcggag ttttcaaaat
     2341 caatcgggtt cactttatgt ggcattgtgc tagagccgat ttcaccggca atagtgcgtt
     2401 gtttgaagtg atttaaggca atgtagcccc acatatcacg atcaaaatca attaaaatcg
     2461 tattaaaacg agccacgcaa tcaaagaact ccgcaatata atcgtgcggt tcaatttggg
     2521 tggtgtatgg gttccacgtc acacctaacg attccacaaa ttcttggctg aaagtgtgcc
     2581 aatcaatctc cggatatgcc gataaatgtg cgttatagtt gcccactgct ccattgattt
     2641 tagcgagaat ttctaaattt tctaactgtt tatattggcg ttgtaaacga tatgccacat
     2701 ttgccatctc tttacccatt gtggtcgggc ttgccggctg accgtgtgtg cgagaaagca
     2761 acggaatatg ttggtaacat ttcgccagct ctgctacagc atcaatcact tttttccatt
     2821 caggtaataa tacttcttca cgagcggttt taagcattaa agcgtgcgaa gtattattga
     2881 tatcttcaga agtacaagca aaatgaataa attcattcac cgcttgtaat tcaggcaacg
     2941 cttcacattt ctcttttagg aaatactcca ccgcttttac atcgtggttg gtggtacgtt
     3001 caatcgtttt aatccgattg gcatcttcta acgaaaaatc agccacaatt tggtttaggt
     3061 aatcgtttgc tttttcagaa aacactggaa cttcattaat ttgtgtatgc gatgccaatt
     3121 tttgtaacca gcgaacttcc accatcacac ggaattttaa taagccaaat tcactaaaaa
     3181 tcggacgaag gctcgcaact ttgtcttgat aacgtccatc aatcggggat aacgcagtta
     3241 aagcggtaag ttccattgtt ttatattctc caaataggta aatgttcggt tagatttgac
     3301 gataaaattt ctgtgcttgg ttaaaaattt tcttgcgtga aaataaaaat tgccaacggc
     3361 ttccgccgac ttgctgccac aaaataccgg ctcgaatacc ggctagtagc gaggctcgaa
     3421 ttttattctg tatatccggg cgaaccaagt aatcttgcat tcccatcacg tgaattttcg
     3481 tacctaacgg gctgataata tcgctataaa tagcggctaa attggcaatc atttgatctg
     3541 ccatcatatt atcttcatac agggctaact ggcgttccac ttgctgtaaa cgctgggcta
     3601 actgttgttt tgcttcaggg tttttattga gtttttggct aagggcgagc aggcttatcc
     3661 aataacgtcc gacttcggta tctaatttgc cgttaccgcc gccagtttga gctaatgcgg
     3721 tttctaagcc catttttaaa tgggaaagat catcgccaaa tacacttaac gttgattccg
     3781 gctgagtgat taacaggctt tctaaagcac acttaaaagc ctctctgtct gccatgcctt
     3841 tatgtgcaaa ttgttgcact aacgtcgccg cttggcaaac gccggcataa gcaagggtaa
     3901 tatcgtggta atccgccatc tctgtaaaat tcaatatgat aaaacattag tgggaatata
     3961 gcattttttc agcttctttt gaatggcaaa aggcatttat tctattgaaa aataaatgcc
     4021 ttttcattaa tacgcaattt gctgcaaaaa ccatcctacc aatcgcccat tatctattcg
     4081 ataaaccaca tcaaatttat ccagtccgct ttggcggtga gtgatcataa ttaaggtgcg
     4141 atcacagcag tgagtcaaaa ttaggtttat aatctgctgt tcggtttctc tgtccagtcc
     4201 ttctgttggc tcatcaagta agactaattc agcagaactc aggagcaaac gggcaagccc
     4261 taaacggcgt tgttcgccac cggaaagcgg tctgccgccc tcgcctaacc ataaattcaa
     4321 gccgtctatt tcaagcaaat gccctaagcc tacttttctt actacttctt gcatttgctc
     4381 atctgtagct tgagcattcc ccattaataa attatctttt agggtttggc taaaaatatg
     4441 cactcgttgg cttaaggtaa ctaattttga gcgtaacatc ggctctgaat attcgtcaat
     4501 tttacaatta ttcagccaaa tttgaccgct tgtagggtcg tagtttcggt ttaacagttg
     4561 gaatatggtc gattttccgc tccctgtttt acctaagatt gccactttct gcccttttaa
     4621 tacctcaaag gaaagattat ttagtacgtt ttgctcgccg taataagcaa agctcacgtt
     4681 ttcaaagcgt acaagcggtt gattttggct caaattttgc cattctgcct tgctaccaaa
     4741 tgttacatta ggctgttgtt cggtaatttc actaatccgc tcggcggcgg taatcacttg
     4801 ccctaaatgc aaaaaggcga taccaatcgg ggaaagaatc tccgccgatg ccattacaca
     4861 gaaaatcact aacgcaatta aggcttcagg gtattttgca gtgggcacat taatcgaagt
     4921 tgaaaccaaa tagatcacca cacaagtcaa aatgccgttg gataacatta tcaagctatt
     4981 tgataagccg gacagtcggc tttctttgct ttgagcgttc agccattggt gttcagtttg
     5041 ctgtagtttt tcgctcattt ggttcaagtt gccaaagagt aaaaattctg catttaactg
     5101 tacccactca ataaattggc tgcggtaatt tgctcggctt tgtattgcat tacgccctaa
     5161 tttcaaaccc aatcgataaa atacggtagg aataacggca agcaatacca atagtgtgcc
     5221 gcaaatgatc agcattaatg gcacggaaac aaaagaaagc ccgattgcca taaatgcaat
     5281 aatcatcact gcactgacaa aaggcgagac taaattcaga tatagggtgt ctaacgtatc
     5341 cacatctgcc accaagcggt ttaacaattc gctgtttcta aagcgattta gcccgcttgg
     5401 actcagtgga atcaattttt taaagacgct agcacgcaaa ttcgccaata cccgaaaagt
     5461 cgcatcgtgg gtgactaaac gttcaaagta acgtgaaatt gtccgcccta ctgccaaccc
     5521 tctcaccccc gatgaagggt agaaaaagtt aaaaataagc gcattgccgg ctagaaatga
     5581 ggcagctaaa aaccaaccgg aaaggctgag caaaccgata cttgccgcta atcctaaaat
     5641 tgccagcaca acacctaata atagtcgccc aaaatgggtt tggtagagag caaaaaacgg
     5701 gaacaacgat ttcattagat ttctccctga tgcactaatt cattatgtaa taattccgca
     5761 aaaaagccgg tgtgttctaa ttcagtgaaa cgacctttct gaatgatttg cccttgtttc
     5821 atcacccaaa tttcatcgca ttgggttaaa tcttccactc gatgagtaat cattagcgtt
     5881 gtttgctctc ggctgagatt atgcagagcg gctaacactt gctgttccga atccatatca
     5941 aggcttgcag tcggctcgtc taacagtaat aattcgtacg ggcgaagcaa cgctcgtgca
     6001 atcgcaatcc gctgtgcttg cccgccagag ataccgatat tactgtcttg cacttgatgc
     6061 tccaacccta aacgtgccac aaattcatcg gcttttgaaa gctgtaatgc cttggctaat
     6121 tcagaatctg tcgcattagg gttgcctatt aaaatattct ctttcaaact gccatgcatt
     6181 aattgcggat tttgccccac ccacgctaat ttcgctcgcc attggcttaa atttaaatct
     6241 cgcagttcgg tgccgttaat cgtaaccgac ccttcataag gcaagaaacc aagtaacata
     6301 ttcattaatg aactttttcc tgcaccactt tgcccaacca aagcgatatg ttggtgtgca
     6361 ttcaactcga aatttaacgc ttgggttaag gctttgcctt gtggtgagaa gatcacgcaa
     6421 tctttggctt gaatcacaag cggttgattt tcaacatttt tttgtaaatt gccaccttgc
     6481 gttttcacct tttcttttaa gaaagtttcg atattatctg cggcactaat cgctgctgcc
     6541 ttgtcgtgat aaaacacgcc aagttcacgc atcggttggt aaaactcagg tgccatcatt
     6601 agacaaaaga agccgataaa taaggtcacg cccgtaccat aatagccaaa atccagctca
     6661 cctaaaaaaa taaagccaaa atagaccgcc gtcaccgcaa tcgataccgc cgtgaaaaac
     6721 tccaataccg cagaggataa aaacgccatt tttagtacgt ccatcgtact gattcggaaa
     6781 tcttcggtgc tttggtagat ttgttcagtc tgcttttccg cttgcccaaa taagcgaatg
     6841 gtttctaaac cttttaattt atctaagaac tgtccgctaa tacgggaaag aatgccgata
     6901 ttacgctgat ttgcctccac tgctttcatt ccggctaaaa tcataaataa aggtaataat
     6961 ggcattgttg caaataaaat agcccctgct gcccagttaa tcgggaatac aaaacagagg
     7021 atcagtaatg gcactatcag cgataaaaat tgctgtggca aatagcgtgc gtagaaatta
     7081 tgtagatttt ccacctgctc gagcattaag gttgcccagc tgcccgccgg tttttgttga
     7141 atgctcatcg gcccgactgc ttccattttg gctagaattt ggcggcgaag atgtaaacgc
     7201 aaggtttgcc ctgctttgaa accgacacgc tcacgcagaa aaatgaagaa tgctctggca
     7261 gcaaaacaac agaataagag cataatttca accagaaagc tattcggtga ctgctttccg
     7321 ataagcattt tgtctaacat tgtggcaatc agccacatct gtccaatcat acataaacta
     7381 ctgaaactcc ctaacagtat gtttaagtgc atatattttt tgatgacttt ttgctgactt
     7441 ttaagccatt tttgtaattc gcgttggcgt tgtttttcca taattttgtc aatttttttg
     7501 ttttttcaac cgcttgtaaa tcgggtttgc cattctacta taaagcaaaa aggttgttaa
     7561 tggcgaaaca ttaacaacct ttcattcagc cttaactaga atagctcgtc aaattcaaag
     7621 cgatacatta aatctaccgc ttggttaata cttgagaccc attgtaagta caatcttggc
     7681 gttaaattat agcgtaaggt taattcggta agcggtgcaa aaatgcccac gccatacttc
     7741 actctgaatt taggggcaag gctggcactt acttctactt tggtgttatc gccaatacct
     7801 gcggtggtca cgcttaaatc tttgagtccg aaagtgctac caacatcacc caccaattta
     7861 ctgctttttg ataagctcat actgagtaat gccgctgcga ctgcattact ggagctggta
     7921 tcgccactgc tttccaatga acgaccagtt aaaatatagg aaagtgcttc attttgcgac
     7981 attgccggat cagagaatac ttttaccgtt ggattgtctg ccaaaccggt cactcttacc
     8041 ccagctgtaa tggtcggatc ttccattgcc tccggattgc gtatagcttc aatatctaag
     8101 gttggctgag acggtgagcc ggcaaaacta atatcacctt tacgaatcag caagtcttga
     8161 ccataagcgg caaatcgtcc ttcacgcaaa tgaacttgcc cgtaaagccc taaaccttgt
     8221 ttgccttgtc tgacagacaa taagccattc acgttactgt ttaaaccata ggctttgagt
     8281 ttgacatcat taccgatgtt aatcttaata tctgcattaa ttgccattcc acctgaggtt
     8341 tgagccggaa tattacgttg gctaaacggc actttatttt tcacagagcc gtccataatc
     8401 acctcatctc cacttacact gaccgcactt tcaggtaact gctcgacttc aattcttgcc
     8461 caagggatat ctacatttcc acttaaggtt aaaagagttg gcgttgccgt cacttgaata
     8521 tccgggctaa ctgccacttt tgcgatatta ggaatatcca cttggaaacg gttagcttga
     8581 gcgtgaaccc gtgttttcca cgcatttaga tctcgccagt ctgcatcacc ctccaaacgc
     8641 agttcgcttt gtttggtttg taatttaccg cttaaagtcg atgttgcccc gtggaaattc
     8701 attgctaaat tcccacctgt aatatcaaac ggcattgtaa cagagcgagc ttttaactct
     8761 gtaacgttta gatttccgtg caataacgga gaggttgcgg tgccgctcac ggtaagacga
     8821 gcattcacat tgccatcaac cgaatcgccc cgttcaagta gcggtcgaat cagctttaag
     8881 cttaattggt caagatgaat attgccggat aacgcacgag ccttacttaa atctttcatc
     8941 actaaatcag tcgaaagtct gccgttattt tcgaccctta aatcagattt aagggttaaa
     9001 ttattgtcag acattttcag atcaatttta agcggtgaaa cggtgagtgg gaaacttttt
     9061 cccccctcca ttttttgaat aaatttaatc gcattagagg ttaaatccag attcacttga
     9121 ggctgtttgt ttttaaacca agctgcatcg cctttggcat tcacaatccc tgaaagctgg
     9181 ctgtttttct ccaaaaattg ttgcaatacc gctaaatcaa agttacggat ttcaaaaggc
     9241 actttaccct caacaccggc attaaatgca gtcgggaaac aaagattaat tttccggtta
     9301 tgccaacaat gtgcggaaat attggcgtta atggctttat tgtcatattt cacattcact
     9361 gctttatctg cttggaattg accaaactcg gtggattgaa tcgaaacttg gcttaattgc
     9421 ccttcccaaa tttctttcaa acggtcaaat tttccagaaa tttgcaaatc agcaccaacc
     9481 ggattgcctt ttgcacttaa tttcaagcta tgattttctt catttccttt tagcaataaa
     9541 gtggcatttt ccactttgat ttcattatag gcaaattgac gtaaccccaa ggttaaatcc
     9601 ccttgaatgg ttttctcagt ggtaattttg ccttttgcgg ttaaattttg cagtttaaat
     9661 tgctcatagc ctacatttga ggccactaaa tctaaatcaa tgttaggttc tgaaacctta
     9721 cccgataatt tggcattacc attcacgctg gcttttaaat tcggcactaa cccttgcaaa
     9781 ttaggggctt taatatccgc atagaaatca gatttttcgc ctaacacgcc ttttaaatcg
     9841 atcttatttt caccatacac cagcgataag cccggaacat ctaaaagttg ttgatgatcg
     9901 gcttttaaat taccgcttaa gtgtagattt ttctgtaata acgaaccttt aatatcaata
     9961 ttcgagacgt ccgctttcca tccgctagct gaattgcccc gtcctgcata accttcggaa
    10021 gataaagaac cggacaatac cgccggccag tctgccagta gggatttagt gttaatatgc
    10081 tgaagtttca aatttgaacg ccacgcaacg ccctcgctcc aatccactaa accgtcgatc
    10141 ttcgccgtac cttccagcgt attgagactt aaatcttgta tattaaaatg ggttaattcc
    10201 cctttgccag ttaaactagc cgagcttgca ggcacgccca ttcctttggc actgacttta
    10261 gcctccaaag cgtaatccag gagattgcca ttaagcctca tggcgatatt ctctaaactc
    10321 agcacatcac cattttgctg aggagcaaac ggataacgca ttgactcact cttcagcgat
    10381 aaatcaaagg gagttttcgc ctgagccagt gcaacattgc cctccaacga ggcgctaatt
    10441 gcaccagccg tcttcacttt taggctggtt ttgtcatata gctcaccgga aagagaggct
    10501 tcaatttgat tgaacggcaa ttttactttc gccgtttttg cctcgtgacc acttagcgtc
    10561 caacttagcg gataattatc ttgtaacgat aaagaaccgc ttgcggttag atccccttgg
    10621 tcgcttttga aatctaaact ttttaggttg atatgctgat cttttgcttg agcctgaagc
    10681 tgtaaagagg caacatttaa caatgagatc ggctcaatca aattcccttt ttcatcttta
    10741 gctttttgag caatcgctac attcgcaata tcaatctgtt caatattgag atcaagaggc
    10801 aaactaaagc gaatattttt atccaataac ggctgtgcta attgtgcttt aatcgctgcc
    10861 caatccactt tattatccac ctgttcggcc tgtgcaacag tttttctctg taattggtga
    10921 aagccctcta cgactttttc actggttttt tccaccaccg cctcttgaat cactttatca
    10981 gcaatctctt gtggatttgt cgctgataac gcctctgttt ttgcattttt ttgtgcaaaa
    11041 tcgaccgctt gttcagtatc agtttcgtct aactttaagg ctaaaagcag atttagcccc
    11101 tctaactgtg tcgggtgaac ggtaatagcc cgccctttac cagaaagtgc agtgtggaaa
    11161 tgattaagat gaatatccat atcatcaact ttgaccttga tattctctaa attgatattt
    11221 tgagctgaaa tccccaatgg caaatttaat tcggtgaaag gttcggtctc ttcttttggc
    11281 tgagaagggg gtaatttagt cgtatcaatc aacacctctg tatctttaag gctgagatta
    11341 ttcaaacaca tttcatagtg gagcaagcag ctaaaaccaa tatgcagatc tgcttgccca
    11401 accgttacat tcacaccgtc catcacaaat cgggcatcag aaagctgtaa cccatcttgc
    11461 aggctacctt gcacttgccc aagttgtaat ggctctacga atttatctac ccagcttaac
    11521 actttacgtt gcccgtaacc tgtgcataga aagagaatgg cgagtaaaag tagaataaac
    11581 gtcagacaaa agccgtgctt caaaaaccgc cactttgatt ttttaggctg tgttgcttgt
    11641 ggctcttgtg ctgagtccgt ttgatttgtg gctgtttgtt tattttcgtc agtcataatc
    11701 gtgcatattt atgaggtgat aaaaagtaaa caagcggtat gcgaaatttt actgaaattc
    11761 gaccgcttgt tttcatttac agatcggaac ctaaaccaat ataaatttgt attcctttgg
    11821 aattatctgg tgattttacc ggcgtggcga catcaaattt aatcgccccg ataggtgatg
    11881 cccaacgcac ccctaagccc gtaccgtagt agagatcctg agttttgaat ttattcgatg
    11941 ccaagcctgc atcataaaaa attgcacccc accaattagg gtaaacctga tactgatatt
    12001 ctactgtgcc tgtggctaaa tgagagcctc cgacacgttt gccattactc ggatcacgag
    12061 gggaaatatc cttatagcca aagccccgca cactcatatc gccaccggca aaatagcgta
    12121 acgccggtgg aatacgttca aattgattgg ctttcaaata ccccaactcc gcccttaaat
    12181 agaaccgatg gttatcaaag taagttctcg cccaagatgt cgaggcttta taactataaa
    12241 agtttacatc tgaacctact gcacgactcc cccagtttac cgttaatttt tgtgtatccc
    12301 cccataatgg gtaaggcttg ccatctgttc tggttcgatt caatgatgcc gttgggtaaa
    12361 ctaataaggt tttaaatcga tcattagctt gagtaaaaga atcgtaacgg gcttttaaac
    12421 caacagaaaa tgaccaaccg gtttcgtgag tccagaaacg ttgtaagcca agggtggcag
    12481 cggttgattt tgtatcgttt tgatcttcgt tttctaaagt gccggataat tggtaataat
    12541 aatgtagcgg atcttctcta agtggaatat tataaccaaa ttcaaatgtc tgctccggct
    12601 tagaaacgta cgttcggctt tcaatactat gacctcggct atttaaccaa ggtttcttcc
    12661 agttaagctg taaccttggg ccaacttcag tttcgtagcc tatcccaatt tccacttcat
    12721 tgcgtttttt aggctgaaat aacacattta aatccactaa tttatcctgt tcatttaaat
    12781 gtggctcaac catcactgat gaaaaccaat tactggaaag gtaatcttgg gttaaaatcg
    12841 aaagatcgtt agctaaatat ggctcaccgg aatgaatttt aagaatattt tgcaaatatt
    12901 cttctttgat ttggctatca acaaaccgga tttgtccata atggtatcgt tttccgctat
    12961 catagcctaa acgccaatcc gctttatgat cggtcggata aacttccaaa cggtgataaa
    13021 gccaatgacc gtcaaaatac cctttactaa aagcggtact ttcaatatca cctttaaaat
    13081 tatcataggt tttatggttt aaacgactac cttgttttgg ggctttactg actagatgag
    13141 cgaaatcttc gtccgttttt gcatcgcctt gcaaaacaat atcggtttca tcaattttta
    13201 ccgtctcttt atctagctca acatttaata ctaagagcgg tttctttccc tcctgaggta
    13261 gctgcacaaa ttggtatcgg ctattataat agcccaaggc tctcagagct ttatctactg
    13321 tggtttgcac caaatattga taacgctctg aaccatctgc atattcattg gcaatttgat
    13381 ctaaatatag ctccacattt ttcttggcat ctttatcctg aatgcctttg acttctaaag
    13441 tgactaatgg ctctatttct ggctcatcat cactatcttc cgctaatttt tcttgcagat
    13501 tttcaactcg ctcacgatct acgtcagaca gcctttgctc ttgatttgct tgtgccattg
    13561 aagaaaaagc cactaaacag cagaatgtta aaaaatgtag ttttgtattc attagtaacc
    13621 tttttattct tttggaatgc cacgcaaaag cggaatactg tcataaggcg gaacagaact
    13681 taacgctggc tcttgctcaa agagcataat gaatggtttg tcaggcactg ttttttcatt
    13741 acgccacaag tttccaacac tcaccaattc aatatcttga ggtagttgtg ctaaattttg
    13801 cttcagtaca ctaatcgtat tcttgcgagg gtgtcctatc accacctcta ccccgttttt
    13861 tcgtgcgtga ttaattgcat gtgcaaactg tttttgtaca tcagcaagca atcgctatca
    13921 tctaaaaaaa tgtctctctc cagcgtatta ataccaaatt gacgagccgt tttagccgcc
    13981 acactactgc caatagtttt actgtctaaa aagaaaagcg tattttcttt taacactttc
    14041 atcaaatgtt gcattgtagc agagtctgaa gttgcgccac tgcccatatg gttatttaaa
    14101 ccaatagcat atggtacttg accacgagaa gtatgaataa gtttccgaat tttctcttcg
    14161 ttatcaccaa tatgaatacc ccccctcttc aattggctgt ttcgatttag gttccattgg
    14221 caaatgaatc aaaatatccc gtttttggtt ataagcgtcc tttgcccgtt ctgttgcata
    14281 gggtgcaaca ggaataatcg ccacgcttac cgccttgggt aaggctaaga tttctcgatc
    14341 ttcttttatt cgatagccca catcatcaat tacaatcgcc atttgagctg cttgtacaag
    14401 cggtgtaaaa agcacaaaca tttgcaaaaa aatgttaaaa ttcaaccgct tgtcaatttg
    14461 tttaaacatc tatttcacca aatttagagg attaatcggc ttgcctttac gggtaacacc
    14521 aaaatagagt gcagaacggc tttgtccccc actgttacca accttcgcaa taacctgtcc
    14581 gccttgcact cggctaccct tgctgactga aatagattgg ttatagccat aaagggaaat
    14641 atcaccattg ccgtggtcaa tcgcaaccat attgccatac cctgcaagcc attgagccat
    14701 tatgactcgc ccgctcgcca ttgctctaac cgaagtaccg gcagaagcac caatcactac
    14761 accgtgccaa ttattaccaa aaggagtgat aatattgcca gacaccggca tcgtatatcg
    14821 accagccttg atcggtgttc tctcaacatt ctcagttgtt ttggactgac cggattttgc
    14881 ctgtttttcc cgctgttgtt cacgtttttc tgcttgctga gcctctcttt gcgcttgttc
    14941 taattgttgt tttagtgcct gttcattgct gcgtaaattc tctaatcggc tttgatcctg
    15001 ctcaagcgtt ttatcgatta aacgtaaggt actttcacgc tggctttgta ctttctttaa
    15061 atctttctct tgtttctttt gttcattaag ttgcgtttga tgatcttttt gctggccttt
    15121 caattcatca cgtctggctt taagagcagc ttgggtttta cgaatttcaa caatcgcatc
    15181 aatgcgaata ttattcatat gctgataata agcggtcatt ctttccgcat tttttgcctc
    15241 ttcagacatt aaacgctcta agaccgaagg gttcacccca gaacggtagg cagagtccaa
    15301 ctgctctttt aatttttctt tctgctgttt ttccaacttt tctaaacggg caatttcagt
    15361 ctcagtcgct ttaatggtct ggcgaacttc cgttaaagac atttcggttt tttgtaattt
    15421 atccaacacc ttccccattt ctacttcttg gttttttagg gtagattgca acgcattacg
    15481 tttttgcttt tgttcattaa tttttgaggt ttgctgttgg attttttgct gaatctgatt
    15541 aagattattt gcaaaagaaa aaggggtaga aaagcttatt accccactga aaatagcagt
    15601 ccaacagtaa gaatttattc gtttcaccag cataccttta tttcttcaaa atattattaa
    15661 ggttatattc taatcggtta tttgcttaag atcagctttt tagtgaaaga atttaactat
    15721 ctatttcaaa atataaagaa ttttgctata aatagcacaa tttcaaatcc attaatttta
    15781 ctcaaagcta aggagtcatt atgcaattag tatttatccg tcacggtttc agtgaatgga
    15841 atgctaaaaa cttatttacc ggttggcgtg atgttaattt aacagaacgt gggattgaag
    15901 aagcaaaatc tgccggtcaa aaattaaaag cagccggttt tgaatttgat attgcgttta
    15961 cttcggtttt aacccgtgcg atcaaaactt gtaacatcgt attagaagaa tcagaccaat
    16021 tatggatccc tcaagtgaaa aactggcgtt taaacgagcg tcactacggt gcattacaag
    16081 gtttagacaa aaaagaaact gctgaaaaat atggtgatga acaggttcac atctggcgtc
    16141 gttcttatga catctctcca ccggatttag acccacagga tccaaactca gcacataacg
    16201 accgccgtta tgctaactta ccaaaagatt taattccaaa tgcagaaaac ctcaaaatca
    16261 cccttgagcg tgtgttacca ttctgggaag atcatattgc acctgcatta atttcaggta
    16321 aacgcgtatt agttactgct cacggtaact cattacgtgc attagcaaaa cacatcgaag
    16381 gcatttttga tgcagacatt atggacttag aaatcccaac cggtcagcca ttagtgtatg
    16441 aactagatga caacttaaaa gtaatcagca aacgttactt ataattggta aatttccgat
    16501 aaaaaataac cgcttgtagt gcaacacatt acaagcggtt attttttgtt aattttttgc
    16561 aaatggcaaa gaacaaaaat aaaggcgaat catacattcg ccttattgtt ttaaagtaga
    16621 ttagaaatct aacaactctt tctcttttgc tgctaatact tcatcaactt ttttaatgaa
    16681 ggtatcggtg attttttgaa tatcttcttc agctttgtgt tgttcgtttt cgctgatttc
    16741 tttatctttt aataacgctt tgatttggtc attagcatca cgacgaacat tacgaattgc
    16801 cactttgcct tgctcacctt ctgctttaac gattttgatt aaatcacgac gacgttcttc
    16861 cgttaatggt ggaagcggaa cacgaatggt tgtgccggca gaagatgggt ttaaacctaa
    16921 atctgaggtt aaaattgctt tttctaccgc agagattaaa gatttatcaa atacgtttac
    16981 cgctaaagta cgggcatctt cagctaccac gttcgctact tgacgtaatg gagtcgcaga
    17041 gccgtaatat tccacttgaa taccgtctaa taaagaaggt tgggcacgac ctgtacgaat
    17101 tttcgcaata tgccctttta aagcctcaag gcttttttcc atacgatctt gggtatcttt
    17161 cttgatttcg ttaatcattg attttttatc cttttattta gtaactgaga gttaaacaga
    17221 gaaaattaat tattcagaaa tggttgtacc ttcttcagta ccgaatacca cattgcgtaa
    17281 cgcccccggt ttgcccatat taaagacacg aattggcatt ccgtggtcac gagcaagcgt
    17341 gaatgctgct aagtccatta cttgtaattc ttgctcaata acttgcgcat acgttaattt
    17401 attatatagt tttgcatctg cgtattttgc tgggtcttta tcataaacgc catcaacttt
    17461 agttgctttt aataccacat cagctttaat ttcaatacca cgcaagcaag cagctgaatc
    17521 ggtggtaaag aacgggctac ctgtaccggc agagaaaatc accacacgtt tttcacgcaa
    17581 cattttaatt gcttcagacc agttgtaagt gtcgcaaatc ccatttaatt ggaacgcaga
    17641 cattaattta gcatttacct ctgcacggtg taaggcatca cgcatagcca aaccgttcat
    17701 tacggtggca agcataccca tatggtcgcc cactacacgg ttcattcctg ctttggctaa
    17761 ttttgcacca cggaataagt taccgccgcc aagtaccaca ccaacctcaa cgccttcagc
    17821 aagtaattct ttaatctcta acgccatacg atccaaaata gacgggtcga taccaaaacc
    17881 ttcactgcct tgtagggctt caccacttaa tttgagaaga attcgcttat aaataggttt
    17941 gctcatttta attgcctctt aactgtatgg actaaacaca aaattacgtt attatagaac
    18001 aatccaatat aacgataaac taaaaatatt cactacaatg aaatccaaac aacttttcgg
    18061 cattcttgtc gctatttcgc tggcaactgc tgctagctgt ttaatgctaa tcggatcagg
    18121 cttttttcca aatccgagta tctttgaagt cattttgggc gtattcctca tcatcgctct
    18181 tgccagctct aaatggactt attatttact cttactgcca atcagcatcg cttatgcaat
    18241 ttataccccg gttgggataa aattcggtaa accgacttac gagtatatcg cctctatttt
    18301 tgctaccgat atgatggaaa gcaaagagtt tattgctcaa ctgccgattt taaatattgc
    18361 ggcgggcatt gcgattgtac tagcggtcat tttttatcga aaaattacca atcgctatca
    18421 aatccgtttt ttacgcaata aaacattcgt gatttcggct tttgtattaa tgctgttttc
    18481 ccttgcaccg tttaaatttt ttcaggagtt ctacaattcg agcctaaagg taaagaaaga
    18541 attggaagtg ctgaacaatt taacgctaga ttcacaatgg ggacaatcaa ccctcagctc
    18601 aacttcaaac tacgatgatt atgtactgat tatgggcgaa agtgcgagaa aggattatca
    18661 ccacgcctat ggctatccgg ctgccaatac gccttttatg agttcggcaa aaggaacctt
    18721 gattgacggt ttaacctccg gtggcacaaa cactatcgcc tcattacgct taatgctcac
    18781 taagcccgat acggaaaaat gggaagcaaa ttatgggtta accttaattg atttaatcaa
    18841 atctaccggt atcaagacct attggatctc aaatcaaggc tatttgggca attacgatac
    18901 gccgatttcc tctatcggta ataaaagtga tgtcaaaatt ttcactaaag ccggcgattc
    18961 attaaatacc aatgtgagcg attttgaatt gttaccgcat tttgctaaag tgattgaaga
    19021 acctgctcaa gggaagcgtt tcatctttct gcatttatat ggctcgcatc ctattacctg
    19081 cgatcgtctc accgattacc cgaaaatgtt tgacgatgac aaactgccaa aacgctactt
    19141 taatgtaaat tgctacgtgt catctatcaa gaaaaccgat gaattgctga aacgggtata
    19201 tgatttgcta gtgaaaaacc aagaaaatca ccagcgttca ttctcaatgg tttatttgtc
    19261 tgatcacggt ttatcgcacg aggtttcaaa agagaacatt gttatccaca atggaaagga
    19321 tagaagtaaa ttacactacg atattccgct gtttaaaatt tcaagcgatg atacccaacg
    19381 taatgagtat aaagccttta aatccggctt aaacttcact aatagtattg cgaattggat
    19441 tggtatcgaa aatccgttat tagataaaga aattaatctt tttaacgaac aaaacgataa
    19501 aagtgattat gggctaaaga aaatcattga aacgcagtct actgtagaag atccagctgt
    19561 tcctattcct gaaagctact tatataaagc gactaattag caaaagtaag taacaaaaaa
    19621 agcaggcttt aagcctgctt ttttagtttt tagtatcaga aaaattaagc gttaccgcca
    19681 gtgattttcg ctacttctgc tgcgaaatct tcttctactt tctcaatacc ttcacctact
    19741 tctaaacgaa taaagttaga aactgaagta tttactgatt ttaaatattg acctacagat
    19801 tgagatggat ccattacaaa cggctgaccg gttaatgaaa cttcaccggt gaatttagcc
    19861 atacggcctt caaccatttt ttctgcgatt tcttttggtt taccagaatt catcgcaatt
    19921 tcaatttgaa tttcacgttc ttttgctact acatcagcag aaacatcttc tgggtttaca
    19981 aattcaggtt tgcttgcagc aacgtgcatt gctactttct taagctcatc ttcagagcct
    20041 gcacctgcta ctaatacacc aattttcgca ccgtgtaagt attgagcaat aacttcgcct
    20101 tctaagtatt gaacacggcg aatgttcata ttctcaccga ttttcgcaac taatgccgca
    20161 cgtttttctt caaattgtgc ttgtaatgct tcgatagttg tacctttatt tgcaactgca
    20221 aaatcagcaa cttcgttcgc taattctaag aaaccggcat cttttgcaac gaagtcagtt
    20281 tcacagttca tttcaactaa tacgccaaaa cctgaagcga cacgagcaag gattacacct
    20341 tcagctgcaa cacgacctgc ttttttagcc gctttcgctt gaccagattt acgcatatta
    20401 tcgattgcta attcaatatc accgttcgct tcaactaatg cttttttaca ttccatcata
    20461 cctgcgccgg tacgttcacg aagttcttta actaatgatg ctgtgatttc agccatttta
    20521 aattcccctt atttgatatg gtaaaacagg ctctcacctg cctagaaatt tcattgttta
    20581 gggcaggtta aaacctgccc cctcaactga attattccgc agaagcttct tctgctactt
    20641 caacagtttc aacaccgcga ccttctttga ttgcagcaac tgctgaatct aagtaaagtt
    20701 ggatagcacg tgttgcatca tcgttaccag gaatgatgta gttaacgcca tctggagttg
    20761 agttagtatc aacaatcgca aataccggaa tacctaagtt gtttgcttct ttaattgcga
    20821 tatgttcgtg atcagcaccg ataacaaaga ttgcatctgg taaaccgttc atatctttaa
    20881 tgccgcctaa gcttaactct aatttctcta actcacgcgt acgcattaac gcttcttttt
    20941 tagtgatttt gtcaaaagta ccgtcttgag tttgagtttc taaatctttt aaacgcttga
    21001 ttgattgacg aactgttttc cagttagtca acataccacc taaccaacgg tgattaacgt
    21061 agaattgttg gctatctaca gcagctagct taactgcttc tgacgctgca cgttttgtac
    21121 ctacgaataa aactttaccg ttgttagagg caatacgagt taattccgct aatgcttcgt
    21181 taaataaagg taatgttttt tctaagttaa tcacgtgaac accattacga gcaccgaaaa
    21241 tgaatggttt catttttggg ttccaataac gagtttggtg accatagtga acgcctgcgt
    21301 taagcatatc gcgcatagaa acttgtgcca taagaatttt ccttcaaagt tgtttgtctt
    21361 accacttatg taaggcaaga cggggtttag cctccacata tcctagaaaa tcgatctttt
    21421 tataaagaca ccccaattcc tagcttgcaa tatgtgtgtg ttttagtgtt aaaaaataaa
    21481 tgtttatgtt ttctatctta acgaagacaa aaaacggcgt gtattatgcc aaaaaacaaa
    21541 cagcttgcca aatattttct ccattttgcg agcttatcat tttaagaaat gctaaaagcc
    21601 atcatttaaa aaatcaataa caaaaaccgt tctcattatt gactaaaaaa atggggaggt
    21661 ttagaatgtc ttccattctg ttatgaaaac ctgtctgaag gagactatca tgaaaaaaac
    21721 actttctgca cttgctattg ctgcagccac ttttacatca acttcaacgc ttgctgcaaa
    21781 tgaagtgaat gtttattctt accgccagcc ttacttaatt gagccaatgc taaaaaactt
    21841 tgaaaaagat actggcatta aagtaaacat catcttcgct gataaagggt tggttgatcg
    21901 tgtaaaacaa gaaggtgaat taagtcctgc tgatgtgctt ttaacagtag atatttcacg
    21961 cgtaatggaa attgttaacg ctgatcttgc acaaaaaatt gactctaaag tgttagaaaa
    22021 aaatattcct gcacaattcc gtgactctaa cgaccaatgg tttggtttaa ccacacgtgc
    22081 tcgtgttatc tacacgtcaa aagatcgtgt aggtaaatta ccagcaggct ttgactacct
    22141 agatttagct aaaccagagt ataaaggtaa agtctgcgtg cgttcaggca aaaactctta
    22201 caatgtctca ctattcgcgg cgatgatcga acattacggc attgaaaaaa ccaaagcatt
    22261 cttagaaggc ttaaaagcga acttagcaca aaaaccacaa ggtggtgacc gtgatcaagt
    22321 aaaagcaatt aaagaaggca tctgtgatta ctctatcggt aacagctact actacggcaa
    22381 aatgttagat gatgagaaac aaaaatcgtg ggctgaagca gcgattatta atttcccgag
    22441 cggtgaatac ggcacgcaca aaaacatcag cggcgttgtt attgcaaaac actcaccaaa
    22501 caaagcgaat gcagtgaaat taattgagta cctcagcggc gaaaaagccc aaggtttata
    22561 tgcggaactt aaccacgaat atccggtaaa agaaggcatt gagccgtcta agctggtgaa
    22621 aggctgggga acatttaccg ctgataccat caaattagaa gacatcgcca aacactacga
    22681 agcggcatta aaattggtcg atgaagtgaa attcgacgat ttctctgaga aaaaataatc
    22741 aaccgataag cggtcatttt tccacaaaaa tttgcaaaat tttgttaaaa tacaaccgct
    22801 tgcaaaagga cgcattttgc gtccttttat ttttttatga aataacaggc ttaaagaaaa
    22861 atgcgatcac aattttgctg gaaaagtatt gctgccctaa caacttttat cgttttgctt
    22921 cctcttattg caatttgcta tcacgcattg caaggggata atgaaaacct tgctcacctt
    22981 tggcaaacaa tgttgccaat ctatcttaaa aactcaactt tattagtatt aggaaccgtt
    23041 ttccttgcac tgatttttgc tctgcctacc gcttggattg tggcaaatta ccgtttttgg
    23101 gggcaaagaa ccttacaatg gttactttgt ttgcctctcg ctatgcctgc ttatttaatt
    23161 gcctatttat ataccgattt attagattac tcaggtgctt ttcaaggctg gctgcgtagt
    23221 atttttggct ggactcatcc gcaagattac tggtttcctc aaattcgtac attgtatggt
    23281 gcttgctttg tgcttgcgtt agtactatat ccatacattt ttttattagt gcgagttgcc
    23341 ttattagaac aatctgaaaa cttaacacac agtgcaaaaa tgctcggggc ttcgccctct
    23401 cagttgttcc gtcgaattac gctgccatta attcgacctt ctgttgccgt aggcattgca
    23461 ttagtggcaa tggaaacgct tggcgatttc ggtactgtgg cctattttgc cgtgccaaca
    23521 cttactacgg caatttatga ttcgtggcta ggctatcacg atcttggtac tgccagccag
    23581 attgcgatct ttatgttgct aatgattttc ctgtttttaa gtttagagca atatagtcgc
    23641 cgtaaacaaa aaagctatca acgtggctat gaaaagaaat cagcctttaa atttttaact
    23701 ggttggaaat tagcattagc ccttgcttgg tgctggggtt tattggcgtt agcctttttc
    23761 attccgtttg gcaggttgct ctattgggca tacgactatt tcgagcaagc gtggaattta
    23821 gactttgtcc aattttcgct taatagtttg aaagtatcga tcatcgcctc cgtgataacg
    23881 gtactgattg ctctacttct gcatttttct aaacgtttga atacccgcaa aattttagtc
    23941 aaaaatgacc gcttgtcatt acaacttgcc tcactgggct atgcggttcc tggcacagta
    24001 ttagcgatcg gtttactttc gcctttaaca tttgccgatc atcaactcca tgcttggcta
    24061 aaaagccttg aattatcacc cgttggattg attttttcag gctcactctt tgcattagta
    24121 ttagcttata ccatccgatt tttagcaatg gcaattggca gccttgaaac ttcgctaaat
    24181 aaaatctctc catcgctgga tatggcgagc caaacgcttg gtaaaaatgg ctcagcaatg
    24241 ttagtaaaaa ttcatatccc tttaatttat aaaggtattt taactgcagg attaatggtc
    24301 tttattgaga gtatgaaaga gctaaatgct tctctgctac ttcgcccatt taattttgat
    24361 acactcgcaa cctatgtgtt tacctttaca tcagacgagc agcttgagcg tgctgctctg
    24421 cctgccatcg tattggtgtt agtcggttta ctgcctgtta tttggcttac cagatccctt
    24481 atttcgcaat ctgaaaaaga aagataaagg aattttatgt ctgttttaac tattaatcat
    24541 ctcaacctca atttcggcag tacaaaggtt ttacaaaatc tccatcttgc tgtgaatcaa
    24601 aatgaaatta tctgtttgct aggtgcaagt ggctgtggga aaactacact gctcaaagcc
    24661 attgcaggtt tattgccaat tgagcagggc gatattcatt tagcgggtga atgcctaaaa
    24721 agcaaagccg tggaagagcg acaaatcggg ctgattttcc aagattacgc tctttttcca
    24781 cacttgacgg tagccgataa tattcaattt ggcttatcca aattgccaaa agtacaacaa
    24841 aaagcaatta ccgaaaaaat gctttcagtt gtgcatttac aaggctttga aagccgtttt
    24901 ccacacgaac tttcgggcgg gcaacaacag cgtgtcgcta ttgcccgagc attagcctgc
    24961 cagccagaat tgctactgtt agatgagccg ttttccaata ttgacagcca aactcgctat
    25021 gcaatgattc aagagatcaa gcaaatctta aaaagccaaa atgtgcccgc tatttttgta
    25081 acccatagca aagaagaagc ctttgcattt gccgataaaa ttgccgtgat ggatcaaggg
    25141 aaaattattc aatttggtaa gccaaacgag ctataccatt ctccagttaa tccatttgtg
    25201 gcagattttt taggtggcac aaactatctt aactgtaatg taacggaaga caacgtgtta
    25261 tgcagcccta ttggcgagtt ccgactcttc cctgaaatga acgttcagaa agggcgttac
    25321 cgttggcttc ttcgcccaga acagattatc ttaaaaccca atgagctagg tttaggtaaa
    25381 gtggtaagca aactgttttt aggcctatac taccgctatc aggttgaaat taaaggcatt
    25441 gagatagtaa cttaccaatc tcaagagctg aaaattggcg aacaagtgga agtgggcttc
    25501 caagtaagag agtttatcct attttaagga gaatatatga aaattgaagt ttggtcagat
    25561 tatgcctgcc cgttttgtta catcggcaaa cgccacttag agcaagccct agcccaattt
    25621 gaaggcgagg ttgaagtggt tttccgagcc tttgagcttg atccgcacgc taacggcgaa
    25681 ccagagggcg atattcagca acgcctaatg cggaaatatc aaaaaaccgc agaacaagct
    25741 gatgaaatga ttcgttatgt ggaacaagcc ggcaaacaag ctgggctaga tttgcgttac
    25801 cgcaccaccc aatacactcg cacctttgaa gctcatcgct tggcgaaatt tgcgaaaagc
    25861 aaaggtttgg gcgaagtgat gattgaacgt ttattcaaag cctatttttc cgataatctc
    25921 attttggcga aacgcactca acttatcgac ttagcgttag agctcggttt agatcgtgat
    25981 gaagtggctc aattacttac aggtgatgat ttcggccacg aagtacggga agatgaacgt
    26041 atggctcacc gctatggcat taatagcgtg ccattttttg tgattgatga aaaactcggt
    26101 gtgtcaggag ctcaaccgcc aaaggtgttg ctagatgcta tcaaacaggc attgcaaaaa
    26161 taatccgaaa aaagaccgct tgttaagcta aacaagcagt cttattcttc ttataattta
    26221 caatttattt tttggtgatc gcacctaaaa taccacgcat aatttgtttg gtgacttggt
    26281 tgcgtaagtt tctgccaact gaatgcgcca cttggctaac cacttgttcg gtaatcgatt
    26341 gatctttttt cttcttcgag ccaaaaatgg aagagagaat accgccaaag aagtcattgt
    26401 ttgcttcttc tgccgcagcc tgctctgctt taattgcttc tgctttcgca ttgagtaaat
    26461 caaaggcaga ttgattattc acatattggc tgtagtgatg atataactca tcttgtttca
    26521 ctatagcatc acgctcgccc atcgctaatg gtgctaattg gctttttggc ggataaatat
    26581 aggcaatttc tacaggcgtt ggcatacctt tttcatctag caccgaaacc aacgcttgcc
    26641 ccacaccaag ttgcgtgatg gcttctacca catttacctc tttattggct cggaaagttt
    26701 ccgctgctga tttt
//
LOCUS       DANKBV010000019        25988 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.019, whole genome shotgun sequence.
ACCESSION   DANKBV010000019 DANKBV010000000
VERSION     DANKBV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25988
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.019"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(162..1400)
                     /gene="pepT"
                     /locus_tag="PWY43_000628"
     CDS             complement(162..1400)
                     /gene="pepT"
                     /locus_tag="PWY43_000628"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL2135532.1"
                     /translation="MKQSLLNRLLRYTQIHTTSDPKSQTVPSSQQQWDLLHLLKTEVE
                     AMGLSEIYLDPNGYLFASLPANIDKTLPVLGLIAHVDTSPDFNGENVKPQVIEAYQGG
                     NILLNGSGDYLSPTDFPNLNKLIGDTLVTTDGTSLLGADDKSGIAIILSAIEYLQANP
                     HIPHGKIRIGFTTDEEIGRGADHFDVNAFGADVAFTLDGGPLGELQYENFNADAATVT
                     FFGRSVHPGTAKGKMINALERACEFQHRLPPHLTPATSEGREGFIMLHGIEGGIDKAE
                     IHYILRSFERDELAAFGKMLNDTLAEMQQIYGEQCGKVEIVEQYRNMREVLDDYGYLV
                     EIAENAMREQGIEPNLEPIRGGTDGSRLSFMGLPCPNLFTGGENFHGRFEFISVDTME
                     KATKVVIDICRKFADYDFNK"
     gene            complement(1420..1809)
                     /locus_tag="PWY43_000629"
     CDS             complement(1420..1809)
                     /locus_tag="PWY43_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL2135533.1"
                     /translation="MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYG
                     VAKRNNGQLSQFLGYFLSSVKVLDWNYRCAELYGKLRAEMEKNGKVMGVQDQMIASHA
                     LAEECILVSSDQAFKMVPDLKLENWLH"
     gene            complement(1810..2070)
                     /locus_tag="PWY43_000630"
     CDS             complement(1810..2070)
                     /locus_tag="PWY43_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL2135534.1"
                     /translation="MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKQENGDILLSLR
                     SRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQRDPFEVA"
     gene            complement(2186..2398)
                     /locus_tag="PWY43_000631"
     CDS             complement(2186..2398)
                     /locus_tag="PWY43_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL2135535.1"
                     /translation="MRDEYDFSNAVKNPYAKGLKSKSVVTIRIEDDVISYFKQLAEEV
                     NLPYQTLINLYLKDCVEQKRKPTISW"
     gene            complement(2399..2686)
                     /locus_tag="PWY43_000632"
     CDS             complement(2399..2686)
                     /locus_tag="PWY43_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL2135536.1"
                     /translation="MEEINGFEWDSEKALRNQQKHGVSFIEAVSVFYDNNAMLIPDPD
                     HSSREERFLLLGRSNKDNLLVVVHCERNTNIRIISARLAAKRESGQYKGVH"
     gene            complement(2849..6382)
                     /gene="mfd"
                     /locus_tag="PWY43_000633"
     CDS             complement(2849..6382)
                     /gene="mfd"
                     /locus_tag="PWY43_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL2135537.1"
                     /translation="MSLISLNLPKSDGNFQDHQTLGNLVGYSDTLAISQAARAFYGLT
                     VVVTPDTRTALRLEKALPQFAGLPVQLFPDWETLPYDNFSPHQDIISARLSALFELQQ
                     GRQQILLLPINTLMQKVCPPNYLANNVLLIKKGDRFSIQKLRLQLENAGYRAVEQVLE
                     YGEYAVRGSILDLFPMGAEEPFRLDFFDDEIDSIRTFDVDNQRTKTEIAEINLLPAHE
                     FPTDSNGIEHFRVKFREKFGEIRREPEHIYQQVSKGILNAGIEYWQPLFFEEMASLFD
                     YFPAQTLFITFADIQQKAEQFHQDTQNRYESRRVDPMRPLLPPSDLWFAIDEVNRWLK
                     GYPRLTITSEKIRKSAAKMNANVANLPDIAIQSSAKEPFSAFQQFRQKFDGNILFSVE
                     SEGRRETLLDLLAPLGVKPKQVNSLDEAQGLPFSLIISPLDQGFVVETGGHGTRPYDD
                     ELNTNGENDNNYVGAGPVPARNLAIICETDLLGERVQTRREKNRKAVNPDTLIRNLAE
                     LKIGQAVVHLENGVGRYGGLTVLDAGGIKAEYLVLHYANDAKLYVPVASLHLISRYIG
                     GADETAPLHKLGSEAWAKTRQKAAEKIRDVAAELLDVYAKRESQTGFAFAYDKAEFDQ
                     FSATFPYEETDDQKIAINAVISDMCQAKAMDRLVCGDVGFGKTEVAIRATFLAVMNHK
                     QVVILAPTTLLAQQHYENFKDRFANYPVNVEVLSRFKTAKEQKTILQNVAEGKVDILV
                     GTHKLLQDDVAFRDLGLLVIDEEHRFGVRQKEKIKQLRANVDILTLTATPIPRTLNMA
                     LNGMRDLSIIASPPARRLLIKTFVRQSDEMVIREAILREILRGGQVYYLHNDVATIEN
                     CAEKLAELVPEARIVIGHGQMRERELERVMSDFYHQRFNLLVCSTIIETGIDVPTANT
                     IIIERADKFGLAQLHQLRGRVGRSHHQAYAYMLTPHPKTLTKDAEQRLEAMSTIDNLG
                     AGFALATHDLEIRGAGELLGSEQSGQIESIGFSLYMDLLENAVKALQEGREPSLDEIT
                     QHQAEIELRVPALLPDDYLPDVNMRLSFYKRIASSENLQSLNDLKVELIDRFGLLPEA
                     TKNLFAITQLRLSAQTLGIKKIDAGIHGGYLEFKPTAQPDPMKFIALIQKQPAVYSFE
                     GAVKFKFRLALEDNRKRLEFVENLLREVVGG"
     gene            6543..7760
                     /locus_tag="PWY43_000634"
     CDS             6543..7760
                     /locus_tag="PWY43_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter"
                     /protein_id="HDL2135538.1"
                     /translation="MKNKILGSALMIAGTTIGAGMLAMPLTSAGMGFGATALLLVGLW
                     ALLAYTGLLFMEVYQTAPQKDVGVASLAEQYFGLIGRVLATFSLLVLLYALLAAYITG
                     GGSLLAGVMPEMADADMKLKISILIFTAVLGTFVVVGVKSVDGLTRVLFLGKIVAFIA
                     VLLMMLPKAKLENLTAIPLDNLLIISAIPIFFTAFGFHVIMGTINSYLDADIGKIRKS
                     IYIGTAIPLVAYLLWQLATHGVLSQNEFTTILTQDPTLNGLVKATSQITGSTILGEMV
                     RLFSVLALITSFLGVSMGIFEGVGDLLKRLNLPTNRLWLTIATFTPPILFALFYPNGF
                     IKALGYAGLLFAFYGMILPIGLAWKARRQYPNLPYRVIGGNLALVVALIAGIIIMVIP
                     FLIQAGYLPQTVG"
     gene            complement(7840..8346)
                     /gene="hutX"
                     /locus_tag="PWY43_000635"
     CDS             complement(7840..8346)
                     /gene="hutX"
                     /locus_tag="PWY43_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDL2135539.1"
                     /translation="MISIEQQTALRNELAENPGQILEMLAAKYQCTLEEVMLNLPAEM
                     LKVTEGERFAEVLQQIHEWDDAITLISHTEDTIIEFVGKLPSGSISRGFYNFEHKEGG
                     GLQGHLRYENCAKIYLLDRPFMGKRTVSLIFINKKGNAMFKIFVGREKIGGALKEHQI
                     KALYQLIG"
     gene            complement(8401..10479)
                     /locus_tag="PWY43_000636"
                     /pseudo
     CDS             complement(8401..10479)
                     /locus_tag="PWY43_000636"
                     /inference="COORDINATES: protein motif:HMM:TIGR01786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            complement(10446..10829)
                     /locus_tag="PWY43_000637"
     CDS             complement(10446..10829)
                     /locus_tag="PWY43_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL2135540.1"
                     /translation="MVFTSYSIKSKKLCPTILALSVSSILSSQATYAEETTAELSEVN
                     VVAKEDVKASIEKKNKEIIQQELIQNNRDLVRYSPDVGIVNQGRHQKGFAIRGVEDNR
                     VGISIDGVSLFLIQKKIPFINAMVI"
     gene            10983..11501
                     /gene="hutZ"
                     /locus_tag="PWY43_000638"
     CDS             10983..11501
                     /gene="hutZ"
                     /locus_tag="PWY43_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein HutZ"
                     /protein_id="HDL2135541.1"
                     /translation="MTTNRQEVLQNRLGPEIEELKQQIKTIMLATIDKDGVPNVSYAP
                     FVINNGEYQVLITTIARHARNLQEVPKVSLMLIEDESKSRQIFARRRLTFDATVRMIE
                     RTDEEWNNSIAALKARHGDLVDELSNMQDFKLFSFKPTAGLFVKGFGKAFDVGVDDLI
                     NIVHLDQGHQTA"
     gene            11651..14263
                     /gene="pepN"
                     /locus_tag="PWY43_000639"
     CDS             11651..14263
                     /gene="pepN"
                     /locus_tag="PWY43_000639"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDL2135542.1"
                     /translation="MQPKAKLRKDYRAPDFTITDIYLDFQLDPDRTFVTSKLTVVRKN
                     PEATTLRLDGHSFDFLSLKYNGEPFSAFQKDDESLTLNLADVPAERFELEIETALNPA
                     QNTSLQGLYKSGDGFCTQCEAEGFRQITYMLDRPDVLAKYTTKITASKSKYPYLLSNG
                     NHIAQGDLHDGRHWVEWQDPFFKPSYLFALVAGDFDLLQDKFITKSGREVTLEIYVDR
                     GNLDRAPWAMESLKRSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKF
                     VLAKPETATDTDYLDIEAVIAHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFTSD
                     LWSRSAKRIEDVRLLRTVQFAEDASPMAHPIRPEKVIEMNNFYTVTVYEKGAEIIRMI
                     HTLLGEEKFQQGMQLYVARHDGSAATCEDFVKAMEDASGVNLEQFRRWYSQSGTPELT
                     ISDEYDETRKAYRLHISQMTPATHDQMDKLNLHIPLKVALYGEQDGKRIPLQYELLTV
                     SDVLDVTAEHQTFEFHNVTQRPVPALLCDFSAPVRLDYNYTTEQLLTLLKHAENDFVR
                     WDAAQMLYTNELRENLSRYQQHQPLSFSEGLVNALTFVLDNSASLPELTALTLTLPKE
                     TEFAESFKTIDPQGIAVVREFMQHSIAHALQDKLLAVYHQNQCGEYQVVAEDIAKRAL
                     RNACLSYLAFTDLGNALVHKHYNNADNMTDTLAALQAVTKAQLSCRDALLADFEQKWK
                     HDGLVMDKWFMLQATRPDDDVLSIVQNLMEHPSFNFNNPNRLRSLVGAFCGQNPKAFH
                     AIDGSGYRFLVDTLIKLNESNPQVAARLIEPLIKLSRYDNQRQTLMRRGLERLRELDN
                     LARDLFEKIEKALD"
     gene            complement(14323..14595)
                     /locus_tag="PWY43_000640"
     CDS             complement(14323..14595)
                     /locus_tag="PWY43_000640"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2135543.1"
                     /translation="MVAKLFSVYGRVQGVAFRFFTLQEANRLGIKGYVRNRDDGSVEV
                     VAEADEQTIAQLENWLQQGSPAAKVERVIVSDYLGSERFKRFEVRY"
     gene            complement(14649..16283)
                     /locus_tag="PWY43_000641"
     CDS             complement(14649..16283)
                     /locus_tag="PWY43_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700110.1"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDL2135544.1"
                     /translation="MQSKFSLSLLTTSLVLGLSANVFAAKVPEGTTLAEKQEITINNG
                     SEPSSFDPHKIEGVPESQVAYQLFEGLVTKDSDGNIQPGVAESWENTPDYKTWTFKLR
                     QDAKWSNGDAVTAHDFVFAWQRLVNPLTASPYSSYLTYLNVENAQDIIGGKKKPEELA
                     VKAIDDHTFQVQLSQPVPYAVELATHSSLLPVHKATVEKHGDAWVKKGNLVGNGAYTL
                     ADHVINEKIVFERNKNYWNDKETVINKATFLAIANAATDVSRYRAGDLDMTNYALPPE
                     QFATLKKEIPNEVFTARTLSTYYYEINHKRAPFDNVKVREALNLALDRTIITDKVLGQ
                     GQTPTYVFTPPYIHEGEKIQQPAYSKDSMADRKAKALKLLEEAGYSKANPLKFTILYN
                     TNDNHKKIAIAVSSLWKQGTDGIVDVKLENQEWKTFLDTRRNANYDVSRAGWSADYNQ
                     ATTFVNYFLSNSSNNTAFYKSQAYDDAVAESYKATDAAGRAAAYAKAEEQLAKDYAII
                     PIYNYVNPRLVKPYVKGYEGKDPQDDILLRNLYIVK"
     gene            16710..18128
                     /gene="leuC"
                     /locus_tag="PWY43_000642"
     CDS             16710..18128
                     /gene="leuC"
                     /locus_tag="PWY43_000642"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDL2135545.1"
                     /translation="MQQAKTLYQKLFDSHVVYEAEGETPIIYINRHLIHEVTSPQAFD
                     GLRVAGRQVRQVSKTFGTMDHSISTQVRDVQQLEGQAKIQVLELEKNCEETGIPLFDI
                     TKKQQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     KQARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGYVVEFCGEAIRDLSMEGR
                     MTVCNMAIEFGAKAGIVAPDETTFAYLKDRPNAPKGQDWEDAIEYWKTLKTDEGAVYD
                     AEVILEAKDIAPQVTWGTNPGQVIGIDDVIPNPEDMADPVTRASAEKALAYIGLEAHT
                     DMKDVPVDQVFIGSCTNARIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKE
                     GLDKIFIEAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSP
                     AMAAAAAVFGKFMDIRKVSLNA"
     gene            18141..18743
                     /gene="leuD"
                     /locus_tag="PWY43_000643"
     CDS             18141..18743
                     /gene="leuD"
                     /locus_tag="PWY43_000643"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDL2135546.1"
                     /translation="MAGIKQHSGLVVPLDAANVDTDAIIPKQFLQAITRVGFGKHLFH
                     EWRYLDAEETQINPDFVLNFPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIA
                     PSFADIFYNNSLNNHMLPIKLTEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGEKVYT
                     FELDEFRRHCLLNGLDNIGLTLQHEAAIAAYEKNIPAFLR"
     gene            complement(18815..19279)
                     /locus_tag="PWY43_000644"
     CDS             complement(18815..19279)
                     /locus_tag="PWY43_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135547.1"
                     /translation="MNKFKIALMATVAIGLFACTPSPQSNSTKQQAQVGYLKQNISQA
                     ELNNPKDFKRYSYYCKNFATGSSSYLATYFPLWSESRKQENFGIYFQLDGGKVYPFDH
                     LANISLNARATKFEVRYRSYQPIEGSFVELVAREFTSTYYKNNVPWLECREG"
     gene            complement(19303..19809)
                     /locus_tag="PWY43_000645"
     CDS             complement(19303..19809)
                     /locus_tag="PWY43_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135548.1"
                     /translation="MADPHIQSPMDFWDCLTVIIYRSGFVLATLMLLLLPYYPQTAQY
                     GLLVSGTMLASSLHLYAKSFRLIFQFSAWIGLLGAVFNFPLLAFGAMLLVIGGLSYKE
                     YFCFRVFGLNFQPLLVAVLWIAFALEWSLLVQILSLVCGLLLLVLSIQKWRMPLHFDI
                     GDKSKYQI"
     gene            complement(19832..20809)
                     /gene="dusA"
                     /locus_tag="PWY43_000646"
     CDS             complement(19832..20809)
                     /gene="dusA"
                     /locus_tag="PWY43_000646"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDL2135549.1"
                     /translation="MTNEIQPFYRGRFSVAPMLDWTTRHCRYFHRQFSRQALLYTEMI
                     TAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAKLVAERGYAEINLNVGCPSD
                     RVQNGMFGACLMAKADLVADCIKAMQDAVEIPVTVKHRIGIDDLDSYEFLCDFIEKVQ
                     PYSNDFIVHARKAWLTGLSPKENREIPPLDYDRVYQLKRDFPHLKISINGGIKTIEEI
                     KQHLQFVDGVMVGREAYQNPSMLGEIDSQIFNENRPLVTAKEAVEKMFPYIERELAKG
                     VYLNHIVRHMLGAFQHCKGARQWRRHLSENATKPGAGVEVVEQALAFIG"
     gene            21160..22137
                     /locus_tag="PWY43_000647"
     CDS             21160..22137
                     /locus_tag="PWY43_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL2135550.1"
                     /translation="MSRNLFQKRLNIKPYEYPELLEFKDAIRHSYWLHTEFNFTGDIQ
                     DYRTTINDHERHVLTRAMLAISQVEVNVKRFWGELYRYFPKPEMDDVGGTFAESEVRH
                     KDAYSFLLEKLGLNEMFSQIHEIEPLMNRIRYMESFMKDKDAGKGEFVLSLVLFSLFV
                     EHISLFGQFIIMMSFNKHKNLFKGISNAVEATSKEEEIHGKFGIALYEILRDEHSELF
                     TPEFFEDLQTLANQAFEAERGILDWIFEEGDLSFITKATVENYIKNRYNNSLSILGLE
                     PPHDIDPEFLKETEWFDIEILSTKETDFFNKRSTDYSKKMKQITADDLF"
     gene            22124..23791
                     /locus_tag="PWY43_000648"
     CDS             22124..23791
                     /locus_tag="PWY43_000648"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL2135551.1"
                     /translation="MTYFNTDRPDFHWLNEDSRLFLQRGYLLEGTTALDRIRFIAEHA
                     ERKLGIEGYADKFYHYMARGYFSLSSPIWSNFGLDRGLPISCFGSYIGDSIHEIMVTT
                     AEVGMMSKIGGGTSAYFGDIRPRGSLIKNNGKSDGSFNFSKLFDTVIDVISQGTSRKG
                     QFAGYIDIEHGDIDEWLDIHTEGNPIQLMYYGVCVGHDWLESMKAGDPYKRQLWAKLL
                     QRKTETGIPYLFFKDNANAGRPDVYKDKNMTIHASNLCTEIMLPSSNEESFVCCLSSM
                     NLLYFDEWKDTDAPETLTYFLDVVMSEFIEKSQSIPFLDRANRFARRHRALGLGVLGW
                     HSYLQANNIAFDSFQAMQKNNEIFKTLQAKTLKASQELAQRFGEPEILEGYGRRNTTL
                     MSIAPTKSSSFILGSVSPSVEPFKSNYYVKDLAKIKTVYKNPFLEKLLQEKGLDTEEI
                     WTSILQNDGSVQHLEQLSEHEKEVFKTFAEISQLSVIQQAAQRQKYIDQGQSINVMIH
                     PATPARDLNQLYLTAEELGLKSIYYQNSMSAAQVFNRNLLSCSSCEG"
     gene            23795..24214
                     /locus_tag="PWY43_000649"
     CDS             23795..24214
                     /locus_tag="PWY43_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135552.1"
                     /translation="MGNLSHTSPNTIVEVVGEFPEITSSGYTFDKGPIQLRIGKHTAP
                     NKGFGVKHIEAEHMQEILNLGFTSVNEFVKAIIVENAKIFCEFSDLKGNHRPIILKAS
                     IGIVVLERKFLESGTIYSVVTAYRRTNPHGIQIGTMK"
     gene            complement(24449..25210)
                     /gene="nudC"
                     /locus_tag="PWY43_000650"
     CDS             complement(24449..25210)
                     /gene="nudC"
                     /locus_tag="PWY43_000650"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDL2135553.1"
                     /translation="MNKGYWIIVSDFDIALEAGEIPFGSAEELGLSGREKLTIGQYQG
                     SPAYLVKALESDNQREFAYLRSQINRPPELSHLLHRAVSLNHFFQTHQFCGKCGEKTI
                     LASDEIAIYCNACGNRAYPTISPSIIVAVRKGRQILLANHLRHKGTIYTTLAGFVEAG
                     EAVETAVHREVFEETGIKVKNVRYFGSQPWAFPNSLMLGFLADYESGEIQLQEEEICD
                     AKWFDCEESLPEIPQQGTIAFELIQATLKLCRESK"
     gene            complement(25203..25886)
                     /locus_tag="PWY43_000651"
     CDS             complement(25203..25886)
                     /locus_tag="PWY43_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL2135554.1"
                     /translation="MKQPTFKRMTNKRSNFAKNLQKNPQKRPLTFAETTLVLLNKPYD
                     VLTQFTDEQGRKTLKDFITMPNIYPVGRLDRDSEGLLLLTNNGEIQHRLAHPKFDKQK
                     TYWVQVEGVPQPEDLAKLAKGVELKDGLTKPAKVRAIATPDFAWHIAPQIRERKTIPT
                     SWIELIISEGKNRQVRRMTAHIGFPTLRLIRVGIGQFRLGNLGCGEFQVLSENEKRKL
                     FTQLGLKDE"
     gene            complement(25883..>25988)
                     /locus_tag="PWY43_000652"
     CDS             complement(25883..>25988)
                     /locus_tag="PWY43_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL2135555.1"
                     /translation="IVIAWLCFNEVPTLKMIIGGLIILLSVAWANLKK"
BASE COUNT         7537 a         5700 c         5151 g         7600 t
ORIGIN      
        1 aaaacgctac gaacctcgta gaggtctcaa caggtgcata ttctatggat ttcagattta
       61 atgtcaaata aatttgtagg aaatctgtaa aaaatcgttt aattggtgaa aaataacgct
      121 ttttgcaaat ttttcatcaa atttgaccgc ttgcagccgc cttatttgtt gaaatcataa
      181 tcagcaaatt tccgacaaat atcaattaca acctttgtgg ctttttccat cgtatcaacc
      241 gaaataaact caaatcgtcc gtggaaattt tcgccgccgg taaataaatt cgggcaaggc
      301 aagcccataa atgaaaggcg tgagccatca gtaccgccac ggatcggttc taaattcggc
      361 tcaatgcctt gttcacgcat tgcgttttcg gcaatttcaa ccaagtagcc gtaatcgtcc
      421 agcacttcac gcatattgcg atattgctcg acaatctcga cttttccgca ctgttcgccg
      481 taaatctgtt gcatctctgc cagcgtatca ttcagcattt tgccgaatgc agctaattca
      541 tcacgctcaa agctacgtaa aatatagtga atttccgctt tatcaattcc accctcaatg
      601 ccgtgcaaca taataaagcc ttcacgccct tcgctggtgg caggggttaa atggggaggc
      661 agacggtgtt ggaactcgca agctcgctct aaggcgttga tcatcttgcc ctttgccgtg
      721 ccggggtgaa cgctccgccc gaagaacgta acggtcgctg catcggcatt gaaattttca
      781 tattgcaatt cgcccagagg cccgccatca agggtaaagg caacatcggc accgaatgcg
      841 tttacatcaa aatgatctgc gcctcgaccg atctcttcat cggtggtaaa gccgatgcgg
      901 atcttgccgt gcggaatgtg cggattggct tgtaaatact cgatagccga gagaataatc
      961 gcaatgccgg atttgtcgtc tgcgccaagc aaagaggtgc cgtcagtggt gactagggtg
     1021 tcgccaatca gcttgtttaa attcgggaaa tccgttggcg aaagataatc gccactgccg
     1081 tttagcaaaa tattgccgcc ttgataagcc tcaatcacct gtggtttcac gttttcacca
     1141 ttaaaatcgg gcgaagtatc aacgtgggca atcagcccaa gcaccggcag ggttttgtca
     1201 atattggcag gcaagctggc gaacagatag ccgttggggt caagataaat ctcgcttagc
     1261 cccatcgcct ctacttccgt tttcagcaga tgaagcaaat cccactgctg ttgcgagctt
     1321 ggcacagttt ggcttttcgg atctgaagtt gtgtgaattt gggtgtaacg tagtaaacgg
     1381 ttaagaaggg attgtttcat tgtttttcct tactatagtt tagtgtaacc aattctccaa
     1441 tttcagatca ggtaccattt taaatgcctg atcgctagaa actaaaatac actcttcagc
     1501 aagagcgtga ctagcaatca tttgatcctg cacgcccatt actttgccat ttttctccat
     1561 ctctgcccgt aactttccat aaagttcagc acaacgataa ttccaatcta aaacctttac
     1621 actcgataaa aaataaccta aaaattgaga aagctgccca ttatttcttt tcgccacacc
     1681 ataaaccaat tcagcagcag ttacagatga aatacaaagc ctctcaggat taatggactt
     1741 caatttcgcc accacatttg catttccacg aaaaaaatag cttacagtat tcgtatctaa
     1801 catatacatt taagctacct caaaaggatc ccgttgctca aaggattggt ttctctcttt
     1861 tgtatctaaa aatgtttcat cacatacaac agcaggcaat agctcaaaca atcgttgcca
     1921 atgctcggta tttctcgatc tcaatgataa taaaatatcg ccattttctt gtttagaaac
     1981 atagacttgc tctgcatcaa actcaaattc aaccggtaaa cgcactgctt ggttccgccc
     2041 gttacggaat aatctcgcaa ttttttccat cctactttac cctccgatag atagtgtata
     2101 tgccaaagta tatgcctatt ttatacaact ggcaagataa cccattctca aatactcaat
     2161 aacatactga tttcaaatca taatattacc aactaatagt cggtttacgt ttctgctcaa
     2221 cgcaatcttt caaataaaga ttgatcaacg tttggtaagg caaattcacc tcttcggcaa
     2281 gttgtttaaa atagctgata acatcgtctt caatccgaat agtaaccaca cttttgcttt
     2341 ttagcccttt tgcgtaaggg tttttcactg cattagaaaa atcgtattca tctctcattt
     2401 aatgtactcc tttatattgc ccactttcgc gttttgctgc caaacgtgct gaaataatcc
     2461 gaatgttggt atttctctcg caatgcacaa ccacaagtag attatcttta ttgctacgcc
     2521 cgagtagcaa aaaacgctct tccctagatg agtgatctgg atccgggatt aacatcgcat
     2581 tattatcata aaatacgctt accgcctcga taaaactaac tccgtgtttt tgttgattac
     2641 gaagagcttt ctcactatcc cattcaaaac cattaatttc ttccattgcc agtttactcc
     2701 tttttgattg taaataaaat atatttacat ctattttttt aagttataaa cagcctagat
     2761 gaaattactg gattcgcaat aaacaaaaac gatttttacg atacagatct caaaaatcgt
     2821 ttttcaagat tgacaacctt aggaggcttt atccgcccac aacctccctt agcaaatttt
     2881 ccacaaattc taaccgcttg cggttgtctt cgagtgctaa gcggaatttg aacttcaccg
     2941 ccccttcaaa actgtacacc gctggttgtt tttggattaa ggcgataaac ttcattgggt
     3001 cgggctgggc tgtcggttta aattcaagat aaccgccgtg aatgccggca tcgattttct
     3061 taatcccgag cgtttgtgcc gacaagcgaa gttgggtaat cgcaaacagg tttttggttg
     3121 cctctgggag caagccgaag cgatcgatca gctccacttt caagtcgttc aggctttgca
     3181 gattttccga gcttgcgatc cgcttgtaga acgacagacg catattcacg tcaggcagat
     3241 aatcgtccgg caataaggcc ggtacacgca attcaatctc cgcttggtgt tgggtgattt
     3301 catcgaggct cggttctcgc ccttcttgca aagctttgac cgcattttcg agcaagtcca
     3361 tatagagcga aaaaccgatg ctttcaatct gtccgctctg ctccgaaccg agcaattcgc
     3421 ctgcaccacg aatttctaaa tcgtgggtcg ccaacgcaaa accggccccg agattatcaa
     3481 tggtactcat cgcctccaag cgttgctcgg catctttggt gagcgttttc gggtgcggtg
     3541 tgagcatata ggcgtaggct tggtggtgcg agcgtccgac ccgtccacgc agttggtgca
     3601 gttgtgccag cccgaatttg tccgcccgtt cgataataat ggtattggca gtcggcacgt
     3661 caatccccgt ttcaataatg gtggagcaaa ccagcaggtt aaagcgttga tgatagaaat
     3721 cgctcatcac ccgctccaat tcacgctcac gcatctgccc gtgtccgatc acaattcttg
     3781 cctctggcac gagttcagcc aatttctcgg cacaattttc aatggttgcc acatcgttat
     3841 ggaggtagta aacctgccca ccacgcagaa tttcacgcaa aattgcctca cggattacca
     3901 tttcatcact ttgtcgcaca aaggttttga ttaaaagtcg gcgagccggc gggctggcga
     3961 taatcgagag atcacgcatt ccgttgagtg ccatattcag cgttcttgga attggcgtgg
     4021 cggtgagggt cagaatatcc acattggcac gcagttgttt gattttctct ttttggcgta
     4081 cgccgaaacg gtgttcttca tcaatcacta aaagcccaag atcacggaac gctacatcgt
     4141 cttgcaacag tttgtgcgtg ccgaccaaaa tatccacttt gccctccgcc acattttgca
     4201 aaatcgtttt ctgctctttg gcggttttaa agcgagacag cacttccaca ttcacagggt
     4261 aattggcgaa acggtctttg aaattctcat aatgctgttg ggcgagcaag gtagttgggg
     4321 caagaataac cacctgttta tggttcatca ctgccaagaa agtcgctcga atggccactt
     4381 cggttttgcc gaagccaaca tcgccacaca ccaatctatc catcgctttg gcttggcaca
     4441 tatcgctaat tacggcattg atggcaattt tctgatcgtc cgtttcctca taagggaaag
     4501 tggcactaaa ctgatcaaat tccgctttat cgtaggcaaa cgcaaaaccg gtttggcttt
     4561 ctcgtttggc ataaacatca agcaattctg ccgccacatc acggattttt tccgctgctt
     4621 tttggcgggt tttcgcccac gcttcactgc ctaatttatg tagcggcgca gtttcatccg
     4681 ccccaccgat ataacggctg attaaatgga gcgaggcaac cggcacatag agtttggcat
     4741 cgttagcata atgtagcacg agatattcgg ctttgatccc accagcgtcc agcacggtta
     4801 agccaccgta acgccccacg ccgttttcca aatgcaccac cgcctgtccg attttgagtt
     4861 ctgctaaatt acggattaag gtatcaggat tgacggcttt gcggtttttc tcacgccgtg
     4921 tttgcacacg ttcgcccagc aggtcggttt cgcagatgat ggcaaggttg cgggcgggca
     4981 caggacccgc ccctacgtag ttattatcat tttcgccatt tgtatttaat tcatcatcgt
     5041 aggggcgggt cccgtgcccg cccgtttcca ccacaaaccc ttgatcaagc ggtgaaataa
     5101 tcagggaaaa tggcaaacct tgtgcttcat ctaatgaatt gacctgcttc ggcttcaccc
     5161 caagcggagc aagcaaatcc agcaaggttt ctcttcgccc ttcgctctcc accgaaaaca
     5221 gaatgttgcc gtcaaatttt tggcggaatt gctggaaagc agaaaacggc tctttggcag
     5281 aggattgaat agcaatatcc ggcaagttag ccacatttgc attcattttc gctgctgatt
     5341 tacggatttt ctccgaagtt atcgttaaac ggggatagcc tttcagccag cgatttacct
     5401 catcaatcgc aaaccagaga tcgctcggcg gcagtagcgg tcgcatcgga tccactcggc
     5461 ggctttcata gcggttttgg gtatcttggt gaaattgttc agctttttgc tgaatatcgg
     5521 cgaaagtgat aaacagcgtt tgggcaggga aatagtcaaa cagacttgcc atttcctcaa
     5581 aaaacaacgg ctgccaatac tcaatccctg catttaaaat gcctttgctg acttgctgat
     5641 aaatatgctc tggctcacgg cggatttcac cgaatttttc acggaatttg actctaaaat
     5701 gctcgatccc gttgctatca gtcgggaact cgtgtgccgg cagtaaatta atttcggcaa
     5761 tttccgtttt ggtgcgttgg ttatccacat caaaagtgcg gatactgtcg atctcatcat
     5821 cgaaaaaatc gaggcgaaac ggctcttctg ctcccatcgg gaataagtct aaaatcgagc
     5881 cacgcacggc gtattcacca tattccaaca cctgttccac cgcccgatag ccggcatttt
     5941 ccagttgtaa ccgcagtttt tgaatcgaaa aacggtcgcc ttttttaatc aacagcacat
     6001 tgttagcgag ataattcggc gggcagacct tctgcattaa ggtgttgatc ggcaacagca
     6061 aaatctgctg tcgcccttgt tgcagttcaa acagggcaga aagtcgggcg gagataatgt
     6121 cttggtgcgg cgaaaaattg tcataaggca aggtttccca atcggggaac agttgcactg
     6181 gcagccctgc aaactgaggc aaggcttttt ccaagcgtaa cgccgtacgg gtgtcgggtg
     6241 tgacaaccac cgtcaagccg taaaaggctc gggcagcttg gctgatcgca agggtatcgg
     6301 aatacccgac aaggttaccg agggtttgat gatcttgaaa attgccgtca gatttcggca
     6361 gattaagtga aattaaagac atacaagcgg tctgttttaa ataaaaaatt gcaaattgtt
     6421 gcttagttta ctaaagcacg gctgattgtg caaaataagc ggagaaatta tgttatctag
     6481 atcgcattgc gaaaaaattc tgataaaatg ttcccctttt ttaactctta ctcacacata
     6541 ttatgaaaaa taaaatttta ggaagtgcat taatgattgc aggcactacc attggtgcag
     6601 gaatgcttgc aatgccactc acctctgccg gaatgggttt tggggcaacc gcattactct
     6661 tagtcggatt atgggcttta ttggcctaca ccggtttatt atttatggaa gtttatcaaa
     6721 ccgctccgca aaaagatgtt ggagtggcaa gccttgccga gcaatacttt ggcttaatcg
     6781 ggcgtgtatt agccactttc agtttgctcg tactacttta tgccctactt gcggcttata
     6841 ttaccggtgg tggttcactg ttagccggtg taatgcctga aatggcagat gctgatatga
     6901 aactgaaaat cagcatttta atcttcactg cggtattagg tacatttgtg gtagtcggcg
     6961 tgaaatcggt tgatggttta acccgagtac tgtttttggg aaaaattgtt gcttttattg
     7021 ccgtgttgct gatgatgctg cctaaagcga agctggaaaa cttgacagca attccattag
     7081 ataacttact cattatttct gctataccca ttttcttcac tgcgttcggt ttccacgtta
     7141 ttatgggaac aattaatagc tatttagacg cagatatcgg caaaattcgt aaatctattt
     7201 atatcgggac ggcaattccg ctagtggctt atttattgtg gcagctcgct acacacgggg
     7261 tattaagcca aaatgaattc accacaattt tgacacaaga tccgacctta aacggcttgg
     7321 tgaaagccac tagccagatt accggtagta cgattttagg cgaaatggtt cgcttattct
     7381 cagtgcttgc attgattacc tcatttttag gggtttcgat gggaattttt gaaggcgtgg
     7441 gcgatttgtt aaaacgctta aatctaccga ctaaccgttt atggctgacc atcgccacct
     7501 ttacaccgcc aattctgttc gctttattct atccgaacgg ctttattaag gcattaggtt
     7561 acgccggttt actctttgcg ttctatggta tgattttacc tatcggctta gcgtggaaag
     7621 cacgtcgcca atatccgaat ttaccttacc gtgtgattgg tggtaatttg gcattagtgg
     7681 ttgcgttaat tgccggtatt atcattatgg tcattccgtt tttaattcag gctggctatt
     7741 taccgcaaac ggtaggctaa tattgaaaag ggctacgatt caatatcgta gccctttcta
     7801 taagttacaa gcggtcattt ttcgatgact ttttacagat tagccaatca actgatacag
     7861 cgccttaatt tgatgctctt ttaatgcccc gccaattttc tctctgccga caaaaatctt
     7921 aaacattgcg ttgccttttt tattaatgaa aatcagtgaa acagtacgtt ttcccataaa
     7981 aggacgatcc aacaaataaa ttttggcaca attttcgtag cgtagatgcc cttgtaagcc
     8041 tccgccctct ttatgttcga aattgtaaaa tccacgagaa attgaaccgc ttgggagctt
     8101 tcccacaaac tcaataatgg tatcttccgt atgcgaaatc agcgtaatcg catcgtccca
     8161 ttcgtggatt tgctgtagca cttcggcaaa acgttcccct tcggttactt ttagcatttc
     8221 agccggtaaa ttcagcatta cctcttccag cgtgcattga tattttgccg ctaacatttc
     8281 taaaatttgc cccggatttt cggccaattc attgcgtaag gcggtttgtt gttcgattga
     8341 tatcattaaa tgttccttta agtaaagaga gtttcttagc tctctaagta aatttgtaaa
     8401 ttagaaacgg tattcaaaat tagccgagaa gttacgtcca ggttcataat agcgttgtaa
     8461 tccaggatag ccaccgtaag ttgcattttc acgtactcca acagaattaa cgattccacc
     8521 tgttgtattt aaaccacgta aagtatccca agtatgatat ttacggttaa acaaattata
     8581 aataccggca cgtaatgtaa tattctcagt tggtttatag aaaccgtata aatccacaac
     8641 aaaggcttta gcatttaagt gcttccaggt atctaaacct gtctcgtgag aatattcagt
     8701 acaacgaatg tcatattcat ccccccaata agggtaatac ggatttggcg ttctatcctc
     8761 tttaaggcat ttttccggta atgtttttaa atattttgca tctttcgcct ttttagcccc
     8821 caagaatgtg aagcgagaaa atactcccca tttaccttca ggttgttcat aatctaagcc
     8881 aactacggct ttaatcggct gaatagaaag taaatctgcc ccgttagaca tactaccttt
     8941 gctgtaaccg aaggctccaa atagcttcca gccttcaggt attggcatag ctgaatttaa
     9001 attccattta ccactcactt ctagcccgta gattttcgca gaatcaatat tttgcatttg
     9061 ctgtaccggg cgccaaaact ctctaccata tgcttggtaa acatcccagg tttcttgctc
     9121 ccataaaaaa tctttatatt tagtatggtg gaaattaact gagagcagcc caacattgcc
     9181 actaccttgc aaacttaaat tttggcttaa actacgttcc gcttttaggt ttggattgga
     9241 catccaagct cctgcagcat tatcacggta atcaaaatac atttctgatg catttggaat
     9301 acggaagcct gtcccaatgt tatatcctac tttccatgcc tcagtcagat gtttttctaa
     9361 accggtaacc caagttagtt gattaaattt cgcattgtca gtttttacgc agttacgaca
     9421 aacaagacca ttgagttctt gttgttcata cttcgcccaa tcataacgaa tgcctagcgt
     9481 tgcaatccaa tcatgcgtta aatcaatctt atcgtgcaat gaaaaagtgt gctgtttcgt
     9541 tttaattggg tacatcatcg tagaatcggg agaatttacc caaacaccgg ataaataaac
     9601 cgagtcctta tgtaatacat cgaaatctct ctctgcagta gaggctttga aagatagctg
     9661 atgagtccat ttaccaaatt ctaatgcttg gctgtctaaa cggaaattta agcgtttcaa
     9721 ttcagtatta aaaatacgaa tattattgtc acttggtttt ctttgtttaa cagggctcca
     9781 gtctgttgca tcttcagcgc gtgtaccttc atagttataa gcagatgttt cagtatcttg
     9841 ataatctgca tctatcttcg ctagagatat ccattttgac tctgggaaat actcataaaa
     9901 aacatttact gtatcacgtt tactacggtc ttccgcttca cgccaaaaag aagtaatagt
     9961 aacggctgaa tcttcgataa tataattttt attattttgc ccactgtaag aaacaccaac
    10021 gcggtgatta tcattaaatc gataagcaaa tttggctaaa taattatgat gattatgagt
    10081 agaatcatca ggagtttgtt tactactgcg taaagagcga gtttgtagag aatcttcagg
    10141 aagtgtataa cctcctgcac ttttcatttc atgcccatgg cgatttgaat aaaccaatat
    10201 cgcctcagct ttctcaccta aatatccaac accgaatgta ttcaaccatt cattattacg
    10261 agtaccatat cctgtgcgat aaaaagcacc aaatttatta ccgttacgaa caatatcata
    10321 aggctcaagc gtccgataat taactccgtc gcctaaatta ccactacctt gattgaaaga
    10381 atctgcacct ttagatactt caatagtacg agctaattcc ggatctatag attggcgaga
    10441 tgtatttaaa ttaccatagc gtttataaag ggaattttct tctgaatcag gaaaagcgag
    10501 acaccatcaa tacttattcc tacacggttg tcttccactc cacgaatagc aaatcctttt
    10561 tgatgtctac cttggttaac aatgccaaca tcagggctat aacgaactaa atctcgatta
    10621 ttttgaatca actcttgctg aattatctct ttatttttct tttctataga agccttcaca
    10681 tcttctttag caacaacatt tacttcagat aattccgctg ttgtttcttc cgcataggta
    10741 gcttgtgaag acaaaatgct actaacgctt aaagccaata tagtcgggca caatttttta
    10801 gactttattg aataagaggt aaacaccatg attctcccta gatagatttt taatatactt
    10861 tattattaat aatatgtatt atcagttaca tcacatttat agtcaataat gattctcata
    10921 tggattgaaa gttttcactc tttatagtaa tctaaaaaac actatttaaa ttgaggtatt
    10981 ttatgacaac caaccgtcaa gaggtcttac aaaaccgttt aggtccagaa attgaagaat
    11041 taaaacaaca aatcaaaact ataatgcttg caaccataga taaagatggt gtaccgaatg
    11101 tgagctatgc cccttttgta attaataacg gcgaatatca agtcttaatc actactattg
    11161 cccgtcacgc tcgtaattta caagaggtgc caaaagtctc tttaatgcta attgaggatg
    11221 aaagtaagag ccgtcaaatt tttgcccgtc gtcgcttaac ctttgacgca acagtgcgaa
    11281 tgattgaacg tactgatgag gaatggaata atagcattgc agcacttaaa gctcgtcacg
    11341 gcgatttagt tgatgaactg tcaaatatgc aagatttcaa gctctttagc tttaagccaa
    11401 cggcaggctt atttgtaaaa ggatttggta aagcctttga tgttggtgtt gatgacttaa
    11461 ttaatatcgt tcatttagac caaggccacc aaactgccta attgcgaaat aaactcacgg
    11521 ctataaataa tcctatttat agccgttttt attgcgacaa gcggtcaaat tttttggaaa
    11581 atttaccgct attttagtac aataacgaca ctattctatg agctaaacgc aactaaaaaa
    11641 aggaaatcct atgcaaccta aagcgaaact cagaaaagat tatcgagctc ccgatttcac
    11701 tattactgat atttatcttg acttccaact tgacccagat cgcacttttg tcacctcaaa
    11761 actaactgtc gtgcgtaaaa atcctgaggc gacaacactt cgtttagacg gtcatagttt
    11821 cgatttcctt tcgcttaaat ataacggcga gccgttttcc gcctttcaaa aagatgatga
    11881 atctttaacc ttaaatttag ccgatgtgcc tgccgagcgg ttcgagcttg aaattgaaac
    11941 cgcattaaat ccggcacaaa acacctcgtt acaaggtttg tataaatcgg gcgacgggtt
    12001 ttgcacccag tgtgaggctg aaggtttccg ccagattacc tatatgctcg accgccctga
    12061 tgtacttgca aaatatacga caaaaatcac cgctagtaag agcaaatacc cgtatctgct
    12121 ttctaacggc aaccacattg cccaaggcga cttgcacgat ggcagacact gggtggaatg
    12181 gcaagatccg ttctttaaac caagctattt atttgccttg gttgcggggg attttgactt
    12241 actccaagat aaattcatca ccaaaagcgg acgtgaagtc acgcttgaaa tttatgtcga
    12301 tcgtggaaat ttagaccgtg caccttgggc gatggagagc ttaaaacgtt caatgaaatg
    12361 ggatgaagag cgtttcggtt tggaatacga tttagatatt tatatgattg ttgccgttga
    12421 tttcttcaat atgggagcaa tggaaaacaa agggttaaat attttcaatt ccaaatttgt
    12481 attggcaaaa ccggaaaccg caaccgatac ggattattta gatattgaag cggtgattgc
    12541 ccacgaatac ttccataatt ggacgggcaa ccgcattacc tgccgtgatt ggttccaact
    12601 aagcttaaaa gagggcttaa ccgtcttccg tgatcaggaa tttacctccg atttatggtc
    12661 tcgttcggca aaacggattg aagatgtgcg tttactgcgt accgtacaat ttgccgaaga
    12721 tgccagcccg atggctcacc cgattcgccc ggaaaaagtg attgaaatga ataatttcta
    12781 caccgtgact gtgtatgaaa aaggggcgga aattattcgt atgattcaca ccctgttagg
    12841 tgaagaaaaa ttccagcaag gtatgcaact ttatgtggca agacacgatg gttctgcggc
    12901 aacttgtgaa gatttcgtta aagcaatgga agatgcttca ggcgttaatc ttgaacaatt
    12961 ccgccgttgg tatagccaat ccggcacgcc tgagctcacc atttccgatg aatatgatga
    13021 aacccgcaag gcataccgct tgcatatttc acaaatgaca ccggcaaccc acgatcagat
    13081 ggataaactc aatctgcata ttccgcttaa agtagcgtta tatggtgaac aggacggtaa
    13141 acgcattccg ttacaatatg aattattgac cgtaagcgat gtgttagatg taaccgcaga
    13201 acaccaaacc tttgagtttc ataatgtaac acaacgcccg gtgcctgcat tactgtgtga
    13261 tttctctgca ccggtacggt tagattacaa ctataccacc gagcagcttt taaccttact
    13321 caaacacgct gaaaatgatt ttgtacgttg ggatgcagct caaatgctct ataccaatga
    13381 actgcgtgaa aatttaagcc gttatcagca acatcaacca ctcagcttct cagaagggtt
    13441 ggtgaatgcc ctaacgttcg tgttggataa ctcagcaagc ctgccggaac taactgcatt
    13501 aaccctcaca ttgccaaaag aaaccgagtt tgcagaatcg ttcaaaacca ttgacccaca
    13561 aggtattgcg gttgtgcgtg aatttatgca gcattccatt gctcacgcct tgcaagataa
    13621 gttattagcg gtatatcacc aaaaccaatg cggggaatat caagtcgtgg ctgaagatat
    13681 tgctaaacga gccttgcgta atgcttgctt aagctattta gcctttactg atttaggcaa
    13741 cgcgttggtg cataagcatt acaataatgc cgataatatg actgatacgc ttgctgcatt
    13801 acaagccgta accaaagccc agctaagttg ccgtgatgca ttattagccg attttgagca
    13861 aaaatggaaa catgatggtt tagtgatgga taaatggttt atgctacaag ccacccgacc
    13921 ggacgacgat gtgttatcca tcgtgcaaaa tttaatggaa cacccaagtt ttaactttaa
    13981 taacccgaac cgtttacgtt cattagtagg tgcattctgc ggtcaaaacc cgaaagcctt
    14041 ccacgccatt gacggttcag gctatcgttt cttggtagat acgctgatta aacttaacga
    14101 aagtaaccca caagttgctg ctcgacttat tgagccgtta attaaactct cacgctacga
    14161 taaccaacgc caaaccttga tgcgtcgtgg tctagaacgc ctgcgtgagc tggataattt
    14221 agcacgagat ctgtttgaga aaattgaaaa agcgttagat taaaccaagc aatacaagcg
    14281 gtgtcgtttg caaatttttt tacaaatgac accgcttgtt agttaatacc ttacctcaaa
    14341 ccttttaaac ctttctgaac caagataatc gctcacaatt acccgttcca cctttgcagc
    14401 aggtgagcct tgttgtaacc aattttccag ttgagcgatt gtctgttcat ccgcttcagc
    14461 taccacttct accgagccgt catcacgatt tctgacatag ccttttatgc ctaatcgatt
    14521 cgcttcctgc aaggtaaaaa accgaaaagc gacaccttga acccgtccgt aaaccgagaa
    14581 taattttgcg accactcttc ccccataaaa ataggctgct tatttttcaa taaacagccc
    14641 ataatgtctt atttcacgat gtagaggtta cgcagtaaaa tatcatcttg cggatcttta
    14701 ccctcatagc ctttcacgta aggtttcacc aaacgtgggt ttacatagtt ataaatcgga
    14761 ataatcgcat aatctttggc taactgctcc tctgcttttg cataagcagc tgcacgacca
    14821 gctgcgtctg tcgctttgta agattctgct accgcatcat cataagcttg gctcttataa
    14881 aacgcagtat tgttgctgga gttcgataag aaatagttca caaaggtggt tgcttggtta
    14941 tagtctgccg accaacctgc acgagaaaca tcataatttg cattgcggcg ggtatctaag
    15001 aatgttttcc actcttggtt ttctaatttc acatcaacaa tgccgtctgt gccttgtttc
    15061 cataatgatg aaactgcaat cgcaattttc ttgtggttgt cgttggtatt ataaagaata
    15121 gtaaatttta acgggttcgc tttactgtaa ccggcttctt ccaataattt caacgctttg
    15181 gcttttctat ctgccattga atctttggaa taagcaggtt gctggatttt ttcaccctcg
    15241 tgaatataag gcggtgtaaa tacataagtt ggtgtttgac cttgtcctag cactttatcg
    15301 gtaataatgg tgcgatctaa agctaagttt aacgcctcac gcactttcac attatcaaac
    15361 ggtgctcgtt tatggttaat ttcatagtaa taagtagaaa gggttcttgc cgtaaacact
    15421 tcgtttggaa tctctttttt caaggttgca aactgctccg gtggaagagc atagttggtc
    15481 atatccaaat caccggcacg gtaacgtgaa acatcagttg cggcattagc aatggctaag
    15541 aaagtcgctt tattaatcac ggtttcttta tcattccaat aatttttatt acgctcaaac
    15601 acgatttttt cattaataac gtgatcggct aaggtataag ccccgtttcc aaccagattg
    15661 ccttttttca cccacgcatc gccgtgtttc tcaacggttg ctttgtgtac aggtaataaa
    15721 gaagagtgtg ttgccagctc aactgcatac gggacaggtt ggcttaactg cacctggaaa
    15781 gtatgatcat caatcgcttt aactgccagt tcctccggtt ttttcttgcc gccaataata
    15841 tcttgagcat tttctacatt gagataagtt aaatagcttg aataaggtga agccgtaagt
    15901 ggattaacca aacgctgcca agcaaatacg aaatcgtgag cagttacggc atcaccgttt
    15961 gaccatttag cgtcttggcg taatttgaac gtccacgttt tgtaatccgg tgtattttcc
    16021 caactttctg ccacacccgg ctgaatattg ccgtcagaat ctttcgttac caatccttca
    16081 aataactgat aagcaacttg cgattccggc acgccctcaa ttttatgtgg atcaaaactt
    16141 gatggctcag aaccattatt aatcgtaatt tcttgttttt ctgcaagcgt tgttccttca
    16201 ggcactttgg ctgcaaacac atttgccgat aagcctaaca ctaatgaggt tgttaataag
    16261 gaaagcgaga attttgactg catatttgat ctccattgat gaatattggc ttgcaaccag
    16321 ccgatttgtt ctaattaatt tttttaagtc taggcattat tattttgatt gcaaggaatt
    16381 ttttaaaaaa atgtgataga tatcaccttt caatgcaaaa attgcataaa aaaatcccga
    16441 agaaaatagc tctataaact ctgataatgg ctagataact tgctgtaact ttctaattat
    16501 tcagaataat attctgcaca ttattaacga atactatttt taggcttatt tagcagaata
    16561 aaaaccctta tttagcataa aaatactgtt ttactttcat ttttttaatc gttatattca
    16621 ctccatatca gcaagcggtc atttttgcaa aattttttac aaatttaacc gcttgtatta
    16681 aaagttaccc ttttgagttg gaacatttta tgcaacaagc caaaacccta tatcaaaaac
    16741 tattcgactc acacgttgtg tatgaagccg aaggcgaaac gccgattatt tatattaacc
    16801 gccacttaat tcacgaagtt accagcccgc aagcgtttga cggcttgcgt gttgccggtc
    16861 gccaagttcg ccaagtcagt aaaaccttcg gcacaatgga ccacagtatt tcaacccaag
    16921 tgcgtgatgt acagcaactt gaagggcaag cgaaaattca agtattagaa ttagaaaaaa
    16981 actgcgaaga aaccggtatc ccactgtttg acatcactaa aaaacaacaa ggtatcgttc
    17041 acgtaatggg gccggagcaa ggcttaactt tacccggtat gaccattgta tgtggtgact
    17101 cgcacaccgc cactcacggg gcctttggtg cgttagcctt cggtatcggt acttccgaag
    17161 tggaacacgt tttagccacc caaaccttaa aacaggcccg tgcaaaaagt atgaaagtgg
    17221 aagtccgtgg caaagtaaac ccgggcatta ccgcaaaaga tattgtgctt gcgatcatcg
    17281 gtaaaacaac tatggctggc ggtacaggct atgtagtgga attttgtggc gaagcaattc
    17341 gtgatctttc aatggaaggg cgaatgacag tgtgtaatat ggcgattgag tttggtgcga
    17401 aagccggtat tgttgccccg gatgaaacca cgttcgctta cttgaaagac cgcccaaatg
    17461 caccaaaagg tcaagattgg gaagatgcca ttgaatattg gaaaaccctc aaaaccgatg
    17521 agggtgcagt ttacgatgcg gaagtgatct tagaagcaaa agacattgct ccacaagtga
    17581 cttggggaac caacccgggg caggtgatcg gtattgatga tgtaattcca aatccggaag
    17641 atatggccga tcctgttact cgtgcctctg cggaaaaagc cttagcttac atcggcttag
    17701 aagcccatac agatatgaaa gatgtaccgg ttgatcaagt ctttattggt tcttgtacta
    17761 atgctcgtat tgaagatttg cgtgctgcgg cagcagtaat gaaagggcgt aaaaaagccg
    17821 acaatgtaaa acgtattctc gttgtgccag gttcaggttt ggtaaaagag caagcagaaa
    17881 aagagggctt ggataaaatc ttcattgaag ccggtgcaga atggcgtaac ccgggttgct
    17941 caatgtgttt gggaatgaac gatgaccgct tgggcgaatg ggaacgttgt gcctctacgt
    18001 ctaaccgtaa ctttgagggc agacaaggac gtaacggtcg tacccactta gtcagcccgg
    18061 caatggcggc tgcggcggcg gtgttcggta aatttatgga tattcgcaaa gtgtcattaa
    18121 acgcataata ggaggaaaaa atggcaggta ttaaacaaca ttcaggcttg gtcgttcctc
    18181 ttgatgcagc gaacgtagac accgacgcaa tcattccaaa acaatttttg caagccatta
    18241 ctcgagtcgg ctttggtaag caccttttcc acgaatggcg ttatttagat gcggaagaga
    18301 ctcaaatcaa ccccgatttc gtactgaact tcccacaata tcaaggtgca accattttac
    18361 tcgcccgtaa aaatttaggc tgtggctctt ctcgtgagca cgccccttgg gcattggcag
    18421 attacggttt taaagtaatg atcgccccaa gttttgcgga tattttctac aataacagct
    18481 taaataacca tatgttgccg attaaattaa ccgaagagga agtcgaagag atcttccaat
    18541 gggtgtgggc aaatgaagga aaacagatcc acgttgattt agaagcgatg actgtgactg
    18601 ttggcgagaa agtttatacc tttgagttag acgaattccg ccgccactgc ttattaaacg
    18661 gtttggataa tatcggctta accctacaac acgaagctgc gattgctgct tatgagaaaa
    18721 acattccggc atttttacgc taattaaaat acaaaaaaac ggctaacatc tttaaggcgt
    18781 tagtcgtttt ctttttagcc attttctttg gcaattagcc ttctcggcac tctaaccaag
    18841 gcacattatt tttgtaataa gttgaagtaa actctcgtgc cactaactct acaaaactgc
    18901 cttcaatcgg ctgataagaa cgataacgca cttcaaattt tgttgctctt gcattcaaac
    18961 tgatgttggc aagatggtcg aacggataaa cctttccgcc atcaagttga aaatagatac
    19021 caaaattctc ttgcttacgg ctttcagacc aaagtggaaa ataggttgcc aaatatgaac
    19081 tgctgcctgt tgcaaaattc ttacaataat agctataacg cttaaaatct ttcggattat
    19141 tcaattccgc ttggctgata ttctgtttca aatatccaac ctgtgcttgt tgcttagttg
    19201 agtttgattg tggtgagggc gtacaagcaa ataagccaat tgccactgtt gccattaaag
    19261 caattttgaa tttattcata aaaatcccga taaaatatta aattaaatct gatatttgct
    19321 tttatcgcca atatcaaaat gcagcggcat tcgccatttt tgaatactca gcacaagaag
    19381 caacaagcca cagacaaggc ttaaaatttg caccaataaa gaccattcta gtgcaaaagc
    19441 aatccacaat accgccacta aaagcggttg gaaatttaag ccgaatactc taaaacagaa
    19501 atattcttta tagcttaatc cgccgatcac cagtagcatt gccccgaacg caagcaaagg
    19561 gaaattaaac acagcaccaa gtaagccaat ccaagcagaa aattggaaaa tcaggcgaaa
    19621 cgatttggca taaagatgca aagaagatgc caacattgtg ccagatacta ataaaccata
    19681 ttgtgccgtt tgcggataat aaggcaataa aagcaacatc aatgtagcta acacaaaacc
    19741 gctgcggtaa atgatgacgg ttaaacaatc ccaaaaatcc attggcgatt gaatatgtgg
    19801 atctgccata actgcctcct taaacaataa attagccaat aaacgcaagg gcttgttcca
    19861 ctacttctac accggctccc ggcttagtgg cattttcact taaatgacga cgccactgcc
    19921 tcgctccttt gcaatgttga aacgcaccaa gcatatggcg aacgatatga tttaaataga
    19981 cacctttggc aagctcacgt tcaatataag ggaacatctt ttccaccgct tcttttgccg
    20041 ttacaagcgg ccgattttca ttaaaaattt gcgaatcgat ctcgcctagc attgacgggt
    20101 tttgataggc ctcacgccct accattaccc catcaacaaa ctgcaaatgc tgtttaattt
    20161 cctcaatggt tttaatgccg ccattgatgc taatctttaa atgcggaaaa tctcgcttga
    20221 gctgataaac ccgatcataa tctaaaggcg gaatttcacg gttttccttc gggcttaacc
    20281 ctgtcagcca cgccttgcgg gcgtgtacga taaaatcatt gctataaggt tgcacttttt
    20341 cgataaaatc acacaaaaat tcatagctgt ctaaatcatc aatcccaata cgatgtttaa
    20401 ccgtgaccgg aatttccacc gcatcttgca ttgctttaat acaatccgcc actaaatctg
    20461 cttttgccat caaacaagcc ccaaacatac cgttttgcac tcggtctgac gggcaaccga
    20521 catttagatt aatctcggca tagcctcgct ctgccaccaa tttggcacaa tgagccaact
    20581 gtgccgggtc gcttccaccc agttgcaaag ccaccggatt ctcgctcgga tcatattcca
    20641 acaaatcata tttggcgtga ataatcgccg gggcggtgat catttcggta taaagcaagg
    20701 cttgacgact aaattggcga tgaaaatagc ggcagtgacg ggttgtccaa tctaacattg
    20761 gagcaaccga aaaacgtcca cggtaaaaag gttgaatttc gttagtcata aaaatatcaa
    20821 aataaagcgg taaaaaatca gtaaaaaata accgcttgta agcgattcaa aacaagcagc
    20881 catcatactc gaaaaagcca gataaacata gtttcctcaa ggcttcccca tctatctgtg
    20941 cacttttaaa acaaccttaa aacacaatat atagtgtttc aacatcaaat cacacacaac
    21001 atattgaagc aaaaatttac aaaattattt ttctttataa atcaataagc taccaaagac
    21061 caataaaaaa gagtgtttgt caagacttgt cttgaaactt tttttgcata caatcatcta
    21121 ccagttccaa acataaactt taagatagag cctacgaata tgtctagaaa cctcttccaa
    21181 aaacgcttaa atattaagcc gtatgagtat cctgagcttc ttgaatttaa agatgcaatt
    21241 cgccattctt actggttaca caccgaattt aacttcaccg gcgatattca agactatcgc
    21301 accaccatta acgatcacga acgtcacgtg ttaacccgag caatgcttgc gatttcacaa
    21361 gtagaagtga atgtaaaacg tttctggggc gagctatacc gctatttccc aaaaccggag
    21421 atggacgatg taggcggtac ttttgctgaa agtgaagttc gccataaaga tgcttactcg
    21481 ttcctattgg aaaaattagg cctaaatgaa atgttcagtc aaattcatga aattgaacca
    21541 ttaatgaacc gcattcgtta tatggaaagt tttatgaaag ataaagatgc tggcaaaggc
    21601 gaatttgttt tatcgttagt gctgttctct ttatttgttg agcatatttc attgtttgga
    21661 cagtttatta tcatgatgtc gttcaacaag cataaaaacc tgtttaaagg gatttccaat
    21721 gcggttgaag ccacctcaaa agaggaagaa attcacggta aattcggaat tgcgttatac
    21781 gaaattttac gtgacgaaca cagtgaacta ttcacaccag aattttttga agatttgcaa
    21841 accttggcaa atcaagcgtt tgaagcagaa cgtggcatct tagactggat tttcgaagaa
    21901 ggtgatttaa gctttatcac caaagcgacg gtagaaaatt acattaaaaa ccgttacaac
    21961 aattctttaa gcattttagg gttagagccg ccacacgaca tcgaccctga attcttaaaa
    22021 gagactgagt ggtttgatat tgaaattctt tcaaccaaag aaaccgattt ctttaacaaa
    22081 cgtagcaccg attattctaa aaaaatgaaa cagattaccg cagatgactt attttaatac
    22141 agaccgcccg gacttccact ggcttaatga agacagccgt ttattcttac aacgtggcta
    22201 tttattggaa ggaacaaccg cattagaccg cattcgtttt attgccgaac acgcagagcg
    22261 aaaactcggc attgaaggct atgccgataa attctatcac tatatggcaa gaggctactt
    22321 ctcgctttct tcgccaattt ggtcaaactt tggcttagat cgtggcttac cgatttcttg
    22381 tttcggtagc tacatcggcg actctatcca tgaaattatg gtgactaccg ctgaagtggg
    22441 catgatgagt aaaattggcg gtggtacttc ggcctacttt ggcgatattc gtccgcgtgg
    22501 ctcgttaatt aaaaacaacg gtaaatcaga cggctcattt aacttcagta aattatttga
    22561 cactgtgatt gacgtgattt cacaaggaac atcacgcaaa ggtcagtttg ccggttatat
    22621 tgatattgag cacggcgata ttgacgaatg gctggatatt cacaccgaag gcaacccaat
    22681 tcaattaatg tattacggtg tgtgtgtcgg tcacgattgg ttagagtcaa tgaaagccgg
    22741 cgatccatac aaacgtcagc tttgggcaaa attgttacaa cgtaaaaccg aaaccggtat
    22801 cccttattta ttctttaaag acaatgccaa cgcaggtcgt cctgatgtat ataaagataa
    22861 aaatatgacg attcacgcct ctaacttatg tactgaaatt atgttgcctt ccagcaacga
    22921 agaaagtttc gtatgctgct tatcgtctat gaacctactc tactttgatg agtggaaaga
    22981 taccgatgca ccagaaacat taacctattt cctcgatgtt gtaatgagtg aatttattga
    23041 gaaaagtcaa tcaattccat tcttagaccg tgcaaaccgc tttgcccgtc gccatcgtgc
    23101 attaggttta ggcgtgttgg gctggcacag ctatctacag gcaaataata ttgcctttga
    23161 cagcttccaa gcgatgcaaa aaaacaacga gattttcaaa acgcttcaag caaaaacctt
    23221 gaaagcctcg caagaattgg ctcaacgctt cggcgagccg gaaattttag agggctatgg
    23281 tcgccgtaat actacattaa tgagtattgc tccgactaaa tccagtagct ttattttagg
    23341 cagtgtgtcg ccatcggtag agccgtttaa atccaactac tatgtaaaag atttagcgaa
    23401 gatcaaaacc gtgtataaaa acccgttctt agagaaatta ctccaagaaa aagggctaga
    23461 caccgaagaa atttggacgt ctattttaca aaatgatggc tcggttcagc atttggaaca
    23521 gctttccgag cacgaaaaag aagtatttaa aacctttgcc gaaatcagcc aattaagcgt
    23581 gattcagcaa gctgctcaac gtcaaaaata tattgaccaa gggcaaagta ttaatgtgat
    23641 gatccaccca gccacaccgg ctcgtgatct taaccaatta tacttaacag cagaagagct
    23701 gggcttaaaa tccatctact accaaaactc aatgagtgca gcacaggtat ttaaccgtaa
    23761 cctattaagc tgctcaagtt gcgaagggta aataatggga aatttatcgc atacttcacc
    23821 taatactatt gttgaagttg ttggtgaatt tcctgaaata acatctagtg ggtatacctt
    23881 tgataaaggt cctattcaat taagaatcgg caaacatact gctccaaata aaggctttgg
    23941 tgtaaagcat attgaagccg agcatatgca agaaattctt aatctaggat ttacatcagt
    24001 taatgaattt gtcaaagcta tcatagtaga aaacgctaaa attttttgcg aattctcaga
    24061 tttaaaaggc aatcatcgcc ctattattct gaaagcaagt attggcattg tggttctaga
    24121 gcgtaagttt ttggaaagcg gtacgatata ttcagttgta accgcttata gaagaacaaa
    24181 tccccacgga atacaaatag gcactatgaa ataaagtacg ttagtgttat aatcagttcc
    24241 acgcaagagc atttgtagaa aataacaggc ggatttggtt ttctactgtg ggcggcaggt
    24301 ttccgctaac ctgtaatagc tatttaattt ttatattaaa tcctgaactt gaaacagact
    24361 aagggattgc tgcggtagct aagaatacaa acctctctat aatcatatag agaggttttt
    24421 tcttattgat gagcacaagt taagataatt atttactctc ccgacataac ttcaaggtag
    24481 cttgaatcag ctcaaaagct attgtgcctt gttgtgggat ttcaggtaag ctttcctcac
    24541 agtcgaacca ttttgcatcg cagatttctt cttcctgtag ctgaatttca ccgctttcat
    24601 aatccgctaa aaaacctagc attaatgaat tgggaaacgc ccaaggttgg cttccaaaat
    24661 aacgcacatt tttcacttta atgcccgttt cttcaaacac ttcacgatgc accgcagttt
    24721 ctaccgcctc accggcttcg acaaaaccgg ctaaagtggt ataaattgtg cctttatggc
    24781 gtagatgatt ggctagtagg atttgtctgc cttttcgcac cgccacaata attgacgggc
    24841 taatcgttgg gtaagctcga ttgccacacg cattgcagta aattgcaatt tcatcgcttg
    24901 ccagtatggt tttctcgcca cattttccgc aaaattggtg agtctgaaag aaatggttaa
    24961 gcgaaacagc tcgatgcaac aaatgagaca gttcgggtgg tcggttaatt tggcttctta
    25021 aataagcaaa ctctcgctga ttatccgatt ctaacgcctt caccaaataa gccggcgagc
    25081 cttgatattg cccgatggtt aatttctcac gtccacttaa gcccagctct tccgcacttc
    25141 cgaatggaat ttccccagcc tccaaggcaa tatcaaaatc gctgacgata atccaatatc
    25201 ctttattcat ctttcaatcc taattgtgta aacaacttac gtttttcatt ttcagatagc
    25261 acttgaaact caccacagcc taaattgcct aatctaaact ggcctatccc aacacgaatt
    25321 aaccgtaaag tcgggaaacc gatatgtgca gtcattcttc tgacttggcg atttttccct
    25381 tcagaaataa tcaattctat ccagctggtc ggaatggttt tccgctcacg aatttgcggg
    25441 gcgatatgcc aagcaaaatc gggcgttgct attgctctga ctttcgcggg cttggtcaaa
    25501 ccatctttta actccacccc ttttgctaat ttggcaagat cttccggttg tggaacaccc
    25561 tccacttgta cccaataggt tttctgcttg tcaaatttcg ggtgagctaa acggtgctgg
    25621 atttcgccat tattagtcaa aagcaacaat ccttcactgt ctctatctag ccgcccgacc
    25681 gggtaaatat tgggcatcgt aataaaatct tttagcgttt ttcgcccctg ttcatcggta
    25741 aattgggtta gtacatcata aggcttattg agtaacacaa gggtggtttc cgcaaaggta
    25801 agcggtcgtt tttgcggatt tttttgcaaa tttttagcga aatttgaccg cttgtttgtc
    25861 attcttttaa aggttggttg tttcattttt tcaggttcgc ccacgcaaca gaaagtaaaa
    25921 taattaagcc accgataatc attttaaggg ttggcacttc attaaagcaa agccacgcga
    25981 tcacaatc
//
LOCUS       DANKBV010000020        25937 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.020, whole genome shotgun sequence.
ACCESSION   DANKBV010000020 DANKBV010000000
VERSION     DANKBV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25937
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.020"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..677
                     /gene="trkA"
                     /locus_tag="PWY43_000653"
     CDS             <1..677
                     /gene="trkA"
                     /locus_tag="PWY43_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDL2135556.1"
                     /translation="IMIVGGGSIGSSLARDLEDQYRVKIIERNPVRAEKLAEKLSKTM
                     VLTGDASDEELLFEEHIENIDLFLAVTSDDEANIMSALLAKRLGAKKVIILVQRPAYL
                     HLIQGGTIDIAISPQQATISALASYVRKGDILQVSSLKLGVAGAVEIIAHGDATTSKV
                     VGRQIRELKLPQGAIVGAVVRGEEVIIAHKSTMIAENDRVIIFVNDKKQIDEIEKLFQ
                     LGVFFL"
     gene            complement(810..1457)
                     /locus_tag="PWY43_000654"
     CDS             complement(810..1457)
                     /locus_tag="PWY43_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HDL2135557.1"
                     /translation="MSIFQQTNPNRRRYGLAVFIGIIAGIISAFVKWGAEHPFPPRSP
                     IDLFTAACPQPVLDAMNAAADQVAAKTIALQECSRAFLNPPHVFLRDYIGIDPTEAAF
                     TFADHAFNWIGVTHMIFSLVFAIGYCIVAEIFPKIKFWQGIGAGIIANICVHYITFPA
                     LGLTPPVAEWPLYEHISELVGHIFWFWTIEVIRRDLRNRITREPDAEVPLDQPFR"
     gene            complement(1713..3158)
                     /gene="aldA"
                     /locus_tag="PWY43_000655"
     CDS             complement(1713..3158)
                     /gene="aldA"
                     /locus_tag="PWY43_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDL2135558.1"
                     /translation="MMKQHKMYINGEFVPALDGRTLAVYNPATEKAISEVPRGGADDA
                     KIAIDAAEAAQEAWERLPAIERGKYLRQIAVGIRERADEIARTISEEMGKTLSLATVE
                     VHFTADYLDYMAEWARRYEGEIIQSDRPNEHIFLYKKAIGVTTGILPWNFPFFLIARK
                     AAPALVTGNTIVIKPSEDAPNNAILFSEIVHQVGLPKGVFNVVTGYGNEVGPELSGNE
                     KIGMVSFTGSQFAGQKVMEAASKNIIKVNLELGGKAPAIVLDDADLELAATAVVNSRI
                     INSGQVCNCAERIYVQSSIKEQFVQKLVEKMQAVKYGNPLEQQDIDMGPMVNEKGLHK
                     VQEMVTHALHQGGNLVLGGNVVEGTGYYFEPTIIDNVDNSMDIMRHEIFGPVIPVATF
                     DTIDEAIRLANDCEYGLTSSVYTQNINKAMKVISRLKFGETYVNRENFEAMQGYHAGW
                     RKSGIGGADGKHGLEEYLQTQVVYLQFDDKV"
     gene            complement(3425..4576)
                     /gene="fucO"
                     /locus_tag="PWY43_000656"
     CDS             complement(3425..4576)
                     /gene="fucO"
                     /locus_tag="PWY43_000656"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL2135559.1"
                     /translation="MANRLILNETSYHGKGAIQNIVHEVTSRSFKKALVVTDKDLIKF
                     NVAQKVTALLDAAGLPYEIFDEVKANPAVDVIKAGVAKFKASSADYLIAIGGGSPIDS
                     AKAIGIIINNPEFSDVLSLEGVAPTHQKCVPIIAIPTTAGTAAEVTINYVITDEAKKR
                     KFVCVDVHDVPAVALVDPDMMESMPKSLAAATGMDALTHAIEGYITKAAWELTDALHL
                     KAIELIGRSLRGAVEKNPKDVEAMALGQYVAGMGFSNVGLGAVHSMAHPLSAYYDTPH
                     GIANAVLLPYVMEFNKNYTGEKYREIARAMGVKGVDEMSQEEYRNAAIAAAQQLSQDV
                     GIPPKLHQIGVKEADLAALSVDAFNDVCTGGNPRDCTAEELLEVYRIAY"
     gene            complement(4705..4956)
                     /locus_tag="PWY43_000657"
     CDS             complement(4705..4956)
                     /locus_tag="PWY43_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL2135560.1"
                     /translation="MSNTLTDKALVVIAALGGRENIVDVYACITRLRVEVKNTDIVEW
                     DKIKSVGALGVVTSGSVIQSIFGSDAEAYRDEIKRILNI"
     gene            complement(5012..5959)
                     /locus_tag="PWY43_000658"
     CDS             complement(5012..5959)
                     /locus_tag="PWY43_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2135561.1"
                     /translation="MKKSALFTATLFGLSTLFAGSAMAKSDEIAVIVKSANSTFWQNV
                     RKGAETAGKDIGGQYKVTFQGPEAETAIDAQVNMVDNAVNRGVAGIVLAASDPVALVP
                     AVKKAYESGIPVVLIDSGINSDGKYYQSFLATDNRAAGKLAAEKLLAKVKGGKVAVMS
                     FTQGAASAIERTGGFIDEIKAKADYKLVGPYYSDSDMTKALNQTEDVLGSNPDLAAIF
                     GANEPTAVGMARAVKQKGFAGKIVAVGFDGNSDLQNFVRDGTLDGIVVQSSYQMGYKG
                     VDTIGKLIKGEKVEKVIDTGVVYVTKENIDSEEAKGVLY"
     gene            complement(5983..6981)
                     /locus_tag="PWY43_000659"
     CDS             complement(5983..6981)
                     /locus_tag="PWY43_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2135562.1"
                     /translation="MNEKQKEMLRKMAALAGLVLLIIFFSVTNEYFFTSNNIMTVGLQ
                     TSTIALIGIGATCVILTGGIDLSTGSVVALSGVAAAMIVNAGVPVPLGMILGILVGGI
                     CGLINGILVTRMKLPPFIATLGMMMVARGLALYVTNAAPVSGMPESFANLGNGALFKI
                     VEEGANGLPKVVFAGIPYPVIIMIFITVLFTFALSKLKVGRYLYAIGSNEEAARLSGI
                     KTNIVKIYAYVASGLLSGLTGVILASRLVTAQPNGGVSYELDAIASAVVGGTSLMGGV
                     GTIPGTLIGSFIIGVLRNGLNMNGVSSFVQMIVIGLVIIVAVSLDQLRQSKKAGKK"
     gene            complement(6978..8507)
                     /locus_tag="PWY43_000660"
     CDS             complement(6978..8507)
                     /locus_tag="PWY43_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2135563.1"
                     /translation="MQNEVILTMKNISKSFAGVNALKGVELTLRKGEVHALMGENGAG
                     KSTLMKCLLGVYQADEGEIIYKGQPVRFESVLEAQKAGISMIFQELNLIPHLTVAENI
                     FFAREPLKHGLVDKDKMVEESAKLLNFFEIDVDPNDEVRMLSVAKQQMVEIAKALSFD
                     VEVLIMDEPTSALTEKEIDKLFELVDRLRAKGVSIVYISHRMEELKRICNHITIFRDG
                     TYVSNHKFNEITMDEIITKMVGRSLDNHFPPKTAVVTNDIILSIMNAERKGVFEPLNF
                     DLRKGEILGITGLVGAKRTELARAIFGADPLDSGEIFVHENKVSIKDPSDAIKAGIAY
                     LSEDRKLNGVAVRMSIRENITMASMDKVANQIGVISYDEEEKASKTFIDKMEIKTPTI
                     EQKVQNLSGGNQQKVVIGKWLFREAKVMIFDEPTRGIDVGAKYAIYQLLDELAANGVG
                     VIVISSELPEVLGVSDRIIVMREGRMTGMLETKKTNQEEIMHYATGVKNMFAREYGVE
                     K"
     gene            complement(8569..9222)
                     /locus_tag="PWY43_000661"
     CDS             complement(8569..9222)
                     /locus_tag="PWY43_000661"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDL2135564.1"
                     /translation="MDRKILARQIIDTCLEMTRIGLNQGTAGNVSVRYKDGMLITPTG
                     TPYEQMTEDSIVFVDGNGKHEEGKLPSSEWQFHLAVYEARPELNAVVHNHAKNCAAVS
                     ILGEPIPAIHYMIACTGTNHIPCVPYAPFGTHQLADYVREGIKHSKAILLAHHGLITA
                     DKTLEKALGIAHEVEVIAEWYLKLLATGKPIPTLNKEQMDIVLGKFKSYGSWVESEQ"
     gene            complement(9282..9713)
                     /gene="fucU"
                     /locus_tag="PWY43_000662"
     CDS             complement(9282..9713)
                     /gene="fucU"
                     /locus_tag="PWY43_000662"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDL2135565.1"
                     /translation="MLKGIHPAISPDLLKVLAEMGHGDELVLSDAHFPAHSIHQNVIR
                     ADGLSVATLLEGISALFEFDQYVEAPLAMMQAVKGDTLDPSVEERYLNAIKKVNGTAP
                     KIERVERFAFYDRAKNAYAVVITGELAKYGNIIIKKGVTPV"
     gene            complement(9788..11221)
                     /gene="fucK"
                     /locus_tag="PWY43_000663"
     CDS             complement(9788..11221)
                     /gene="fucK"
                     /locus_tag="PWY43_000663"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDL2135566.1"
                     /translation="MPISLIFDCGATNLRTIAINQAGEIVAAHHLPNNTQPDVENPEF
                     HIWDIEEIWSKLMTCAKQTLSQLSEQQRQEIVGISVTTFGVDGALFDKNGKQLYPIIS
                     WKCPRTLPIMENLANYLDVEALYQRNGVGHYSFNTLFKLLWLKENKPEIYQQAESFLF
                     ISSILTYRLTGVQTTDRTMAGTSMMTNIENDSWDKTVLDLLGLTENHFPPMKSAGEVV
                     GTLKGEISQELGLSGQIPVISCGHDTQFAIFGSGAGYNQPVLSSGTWEILMVRTPKAK
                     PQWQFVQNGLTIEFDSQSGYFNPGVQWVASGVMEWFGKRFFSDIANTEHYYRTMIEEA
                     AEVPAGSNGVKLVGNFDGTTAKTGSIIGLSMHITRGEIYRAGLEYMAHRLKEGLDVLQ
                     EVGHFNAESLICVGGGSKNALWNQIRADVLNRPIDVVDFPESTVLGAAMFTFTGAGVF
                     ENPNAAQQAMKPNVKRVEPSSNSQFYK"
     gene            complement(11415..13181)
                     /gene="fucI"
                     /locus_tag="PWY43_000664"
     CDS             complement(11415..13181)
                     /gene="fucI"
                     /locus_tag="PWY43_000664"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDL2135567.1"
                     /translation="MTTLKSTPVKIGIRPTIDGRRLGVRESLEDQTMNMAKAVAQLIQ
                     SEVRHPNGDFVECVIADSTIGGVAEAAACAEKFKRENVGAVITVTPCWCYGSETIDMD
                     PHMPKAIWGFNGTERPGAVYLAAALAGHSQLGLPAFSIYGTEVQEADDQSIPDDVREK
                     LLRFARAGLAVATLRGKSYLSIGSVSMGIAGSIVNQQFFQEYLGMRNEYVDMTEIKRR
                     LDRHIYDAEEFKLAMSWVKTYCKEGIDVNAPENQRSPEEREKLWEDVVKMTIIARDLM
                     VGNPRLAELGYSEEALGHNAIAAGFQGQRQWTDHLPNGDFMEATLNSTYDWNGVRAPY
                     IMATENDSLNGVNMLFGNLLTGQAQIFADVRTYWSEDQVERVTGWRPESGFIHLINSG
                     SAALDGTGQHTDRAGKPTIKPAWEVTEEDGKRCLENTCWCPAVHEYFRGGGLSSQFLT
                     KGGMPFTMHRINLIKGIGPVLQIAEGWSIDLPENVHDILNKRTNETWPTTWFVPRLTG
                     KGAFTDVYSVMANWGANHCVATFGHVGADLITLASMLRIPVCMHNVEDKDVFRPSAWN
                     GFGQDKEGQDYRACANFGPLYK"
     gene            complement(13377..14123)
                     /locus_tag="PWY43_000665"
     CDS             complement(13377..14123)
                     /locus_tag="PWY43_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2135568.1"
                     /translation="MQQRQDKIIAYLAQFDEANVQELAEHCGVSIETIRRDLNKLDKD
                     GLLHRTHGGAVSHKKRDIGRSFTTRQRLNSEAKRSIAENVLEYLQPESVIALDASSTS
                     WNVAQRIPNIPCTVVSSSMRIIYSLSHKPHIKTIATGGIYSEKYDAFYGPLSEQLLTR
                     LKIDLAILSCTGIADGAIWESNEINISLKRKLIANSKQVFLLADHSKFERKDLFRIED
                     LSCIDKLFINQPPPDFLKNYCLAHQIEIII"
     gene            14403..16499
                     /locus_tag="PWY43_000666"
     CDS             14403..16499
                     /locus_tag="PWY43_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL2135569.1"
                     /translation="MKNFKLLPVSAAVLSVLAANAFAETVATDAAVLDTVTVTDNQGL
                     KVQTNVVTTQKKEESTQTDLRGLLKDEPAISLGGGNGTSQYLYIRGMGQNSIDVKVDN
                     TYSDSQILYHQGRHMLDPALVGTVTVQKGAGSASAGIGQTNGAVIAKTVDALDLLKNS
                     DKNFGAKLGTGFSTNHAHNFNATLYGKGEIFDALVSGNLVRDRDYKGGKDYVNQFGTN
                     RVPYSALDKSSFLAKLGATLGDHRFVLSHSNERNEGERLVREEFDAAPVGTKGSRVTL
                     DRQAPANRRMTVQRTNLEWTGKNLGFAQEATANVYQLVQGRWSENETRNGYAGGVDYA
                     TKTKIVTHGANVNFDSALHENVLLKYGVNYRHQETKPYAKFSHQLVNQEKTDTGVYLE
                     AITAPVDKVTLTTGVRYDHFKFKAMDGKKRSEGAFNPSVGIIYEPIQHLSFSASHNYA
                     TRSPRMHDALLSHGRRGVVSIANNTKAEQARSTEIGFNYNDGTFSFDGSYFWQNIKDA
                     LGTSTGRNNHLCPELTPNTNNKECYSEIINAGTIKNRGYELNAGYHNNGFTARVGVAH
                     SKPRFQGDRLSTNPEYASAIGRTWTASLSYRFDQPNLEVGVHHRIIEKVKAEDNYFVM
                     NNNGIPQFGRGGPTGKDGYNVTDITLNWKPLNDDSVNVNFAVDNVANKKYNAHGQRGQ
                     LSARGREFRAGVNYTF"
     gene            complement(16615..16902)
                     /locus_tag="PWY43_000667"
     CDS             complement(16615..16902)
                     /locus_tag="PWY43_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2135570.1"
                     /translation="MAKTLRISSRAQNDIEEILASVIEYTGFESSGIRLQEDIYQKFE
                     TIAYMPSAAGRLREDGTREAFTRRYSIVYTELENEVWIITIIHSSRLYPRI"
     gene            complement(16895..17074)
                     /locus_tag="PWY43_000668"
     CDS             complement(16895..17074)
                     /locus_tag="PWY43_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135571.1"
                     /translation="MFKEKGYDEFLAEKIRLGLEDMQSGNGLSLDESKARTKQLIERK
                     ARELANFEQENIIYG"
     gene            17336..21235
                     /gene="purL"
                     /locus_tag="PWY43_000669"
                     /gene_synonym="purI"
     CDS             17336..21235
                     /gene="purL"
                     /locus_tag="PWY43_000669"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDL2135572.1"
                     /translation="MTIQTFRGSPALSAFRIQQLLQKFQQNQLPVKSVYAEFLHFVAL
                     NRPLVADEENKIKELLHYGPTLEEDEPTGFCLIVTPRIGTISSWSSKATDIAHNCGLA
                     AVERIERGTAYYLEFSETPNESQLTTLKGLLHDRMMETVLDNESQAEQLFAQQEPKPF
                     TSVDILGGGREALEEANVNLGLALAEDEIDYLVENFTALGRNPNDIELYMFAQANSEH
                     CRHKIFNADWTIDGVKQEKSLFKMIKNTFEKTPDYVLSAYKDNAAVMEGSTVGRFFPD
                     QDGQYRYHQEDAHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLT
                     GFSVSNLVIPNFEQPWENPLSKPSRIASALDIMIEGPLGGAAFNNEFGRPALLGYFRT
                     YEEKVNSFAGEEVRGYHKPIMLAGGIGNIRAEHVQKGEIPVGAKLIVLGGPAMNIGLG
                     GGAASSMASGKSKENLDFASVQRENPEMERRCQEVIDRCWQMGAENPILFIHDVGAGG
                     LSNAMPELVHDGERGGKFDLRNILCDEKGMSPLEIWCNESQERYVLAVSPEKLDIFTA
                     LCERERAPFAVIGEATKEKHLTLADTHFNNKPIDLPMNVLLGKTPKMQRDASSKTVKN
                     PPLVTADIDPKEAFHRVLRLPAVAEKTFLITIGDRSVTGMVAQDQMVGPWQIPVADCA
                     VTTASLDSYHGEAMSIGERSPVALLDFAASARLAVAESITNIAATNIGDIKRIKLSAN
                     WMSAAGHEGEDAGLYEAVKAVGEELCPALGITIPVGKDSMSMRTTWEENGEQKSVTAP
                     LSLVISAFARVEDVRKTVTPQLRTDKGDTRLLLIDLGEGNNRLGATALAQVYKQIGDK
                     PADVVNVERLKGFFNAIQILVAQEKLLAYHDKSDGGLITTLAEMAFAGNCKIRAEIDR
                     LGTDDLGVLFNEELGAVIQVKTSDLESVQAVLKAENLANLTYDIGSVAQGDGFEITRN
                     GEMVFSQKRSELRGIWAELTHQMQRLRDNPECADQEFEAKKDPNNKGLSAFLTYDVNE
                     DIALPFINKGTKPTIAILREQGVNSHYEMAAAFDRAGFNAIDLHMSDLISGRRNLNDF
                     NAMVACGGFSYGDVLGAGGGWAKSILFNPMLRDQFSQFFVNPNTLALGVCNGCQMVSN
                     LAEIIPGTENWPRFVRNKSERFEARAAMVKINDTNSLWFNGIAGSHMPIAVSHGEGQV
                     EFKRSEQLENLQKQNLVIAQYVDSHLNPTEQYPANPNGSALGITAIGNTDGRVAIMMP
                     HPERVFRAVSNSWCPEDWTEDGAWMRLFRNARVALKY"
     gene            21595..21777
                     /locus_tag="PWY43_000670"
                     /pseudo
     CDS             21595..21777
                     /locus_tag="PWY43_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            22227..22604
                     /locus_tag="PWY43_000671"
     CDS             22227..22604
                     /locus_tag="PWY43_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL2135573.1"
                     /translation="MDKSQGVVSVNIRGGSGFGRANNAIGPNYMGMLAGKYQFNDSTW
                     IGALFGHSWRKISQNYKIGGGRKVTDSSIDLSKIDDLDKESSTTSPFNAAHLKQKPMS
                     RMAKLEYGDKYQTITLLPKSGSG"
     gene            22756..23448
                     /locus_tag="PWY43_000672"
     CDS             22756..23448
                     /locus_tag="PWY43_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL2135574.1"
                     /translation="MAINNKATTLNLSNTFKVSPFKDGEWEAILGFNRLKNRYSKNRH
                     PAELNYNLENGETSENNDSVGMTAVRKSLYTSTFQPDGEQKFDTFYLDNHFNYGIWGL
                     DINANINTARFIGQRFKYFPFAIRELESDIRQASRNRQFEHLRSLQSQLDNLQKNIVH
                     IFQMRIFLMILNWESLTVLMSIFLIQQQENINELIIQQPCLLIFTIYSRPLLVILKHI
                     EPLILKKYSSLI"
     gene            23334..24110
                     /locus_tag="PWY43_000673"
     CDS             23334..24110
                     /locus_tag="PWY43_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL2135575.1"
                     /translation="MNYSATLSAYIHDLFTPFISYSQTHRAPNIKEIFFSNLGDYGVN
                     TALQPEQAKTTQFGINGFKERVFSEEDKLGFKVLFYNTHLKNYIYNVQHTAVGSAPSL
                     FIFHKNYEKPVNIKGIELEFSYDIGSFYGNLAYARQNTNQPVSFTDASSRVDAASHYG
                     FKTQGFGASKISALPRDYGSLELGTRWFDQRLQIGGVAKYYGKSKRIAIDEFNYTYYP
                     GTHIVKETHRLEETVNKQPIIVDFYVSYEPIKSLILKLEL"
     gene            24714..25208
                     /locus_tag="PWY43_000674"
     CDS             24714..25208
                     /locus_tag="PWY43_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2135576.1"
                     /translation="MSRKYKFHEKSGAYFVSFATVYWVGVFVREIYFQVIVDALDYCR
                     KHKGMILYAYCIMPTHIHLLFQAENQNPSDLIRDFKTFTAKILIQRIKDNLQENRREW
                     LLWMFSRAAERRCNVKQYQFWQHHNQPIEIYSQKFFDEKLNYIHQNPVVSGFVSEPWE
                     WKYN"
     gene            25463..25702
                     /locus_tag="PWY43_000675"
                     /pseudo
     CDS             25463..25702
                     /locus_tag="PWY43_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
BASE COUNT         7500 a         5372 c         5408 g         7657 t
ORIGIN      
        1 ggattatgat tgtcggtgga gggagcatcg gctcttcgtt agctcgggat ttagaagatc
       61 agtatcgggt aaaaattatc gaacgcaatc cggttcgagc cgaaaaatta gcagaaaaac
      121 tatctaaaac gatggtgctt accggtgatg cctccgatga agagttgctg tttgaggagc
      181 atattgagaa tattgattta ttccttgccg tcaccagtga tgatgaagcg aatattatgt
      241 ccgccctgct ggcaaaacgg cttggggcga aaaaagtgat tattttagtg caacgccctg
      301 cctatttaca cctgattcaa ggtggcacta ttgatattgc catttcccca caacaagcta
      361 ctatttctgc tctcgctagt tacgttagaa aaggcgatat tttacaagtt tcttcactca
      421 aacttggggt tgcaggagcg gtagaaatta tcgctcacgg cgatgcaacc acttcaaaag
      481 tggttggacg acaaatccgt gaattaaaac taccgcaagg ggcaattgtt ggggcggtgg
      541 tgcgaggcga agaagttatt atcgcccaca aatccaccat gattgcagaa aatgaccgtg
      601 tgattatttt tgtgaacgat aagaaacaga tcgatgaaat tgaaaaactg ttccaacttg
      661 gtgtattttt cttataaagc caagctccgc attgctacat gaaacaatgt gctttttgtt
      721 tggtaaattt tcatgaaaat ttgaccgctt gtaaaataag aaagccgcaa cgtgattgcg
      781 gcttttgtat aaatcgagct aattttaaat taacggaacg gttgatctaa cggcacttcg
      841 gcatcaggtt cacgcgtaat acggttacgt aaatcgcgac gaatcacctc aatggtccag
      901 aaccagaaaa tatgaccaac taattcagaa atgtgttcat ataacggcca ttcagcaacc
      961 ggtggtgtta aacctaatgc cgggaaggtg atgtagtgaa cacaaatatt agcgatgata
     1021 ccggcaccga taccttgcca gaatttaatt tttgggaaga tttccgccac aatgcagtaa
     1081 ccaatcgcaa agactaacga gaaaatcata tgggtgacac caatccagtt gaacgcgtgg
     1141 tcagcaaagg taaatgctgc ttcagttgga tcgataccaa tataatcacg taagaaaacg
     1201 tgtggtgggt ttaaaaatgc acgtgaacat tcttgtaatg cgatggtttt ggcagccact
     1261 tggtctgctg cggcattcat tgcatctaac acaggttgcg ggcaagcagc ggtaaacaga
     1321 tcgatagggc tacgaggtgg gaaaggatgt tctgcccccc attttacgaa tgctgaaata
     1381 ataccggcaa taatcccgat aaatacagct agcccataac gacggcggtt tggattggtt
     1441 tgttggaaaa tactcataat gaaaatccta atttgagtga taattgtgtt gtgttaatca
     1501 agccatttta tggaatgtga tttcaaaaaa ggctaatggc attctgcact caattaattt
     1561 aaattgcaaa ttaattagcc aaaaagagtt aaccataaat aattattaaa catttactta
     1621 acattagaat atttttgttt gcaattttga tacaaggtta acctaaaaga aaactcccgc
     1681 ttattttgat aagtgggagt ttttagaaga atctatactt tatcatcaaa ttgcagataa
     1741 accacctgag tttgtaggta ttcctctaag ccgtgcttac cgtcagcacc gccaataccc
     1801 gatttacgcc aaccggcgtg gtagccttgc attgcttcaa agttttcacg gtttacataa
     1861 gtttctccga attttaagcg ggaaattact ttcatcgctt tattgatatt ttgcgtgtaa
     1921 accgaagagg ttaagccata ttcacaatca ttggctaagc gaatcgcttc atcaatggta
     1981 tcaaatgtcg caaccggaat gaccggacca aaaatttcgt gacgcataat atccatagag
     2041 ttatctacat tgtcgataat agttggctca aagtagtagc ctgttccttc aaccacgtta
     2101 ccacctaata ccagattacc tccttgatgc agtgcgtgtg tcaccatttc ctgcacttta
     2161 tgcaagcctt tttcatttac catcggcccc atatcaatgt cttgttgttc taatggatta
     2221 ccatatttca ccgcttgcat tttctctacc aatttttgga caaattgctc tttaattgaa
     2281 ctctgtacat aaatgcgttc tgcacagtta caaacctgtc ctgaattgat gattcgagaa
     2341 tttactacag cggtagcagc aagctcaaga tctgcatcgt ctaatacgat agccggtgct
     2401 tttccgccca attccaaatt gactttaatg atattcttgc tggcggcttc catcactttt
     2461 tgtccggcaa actggctacc ggtaaaggaa accataccga ttttttcatt gcctgaaagt
     2521 tccggcccga cttcattgcc atagccggtg acgacattaa ataccccctt aggtaaaccg
     2581 acttgatgca cgatttccga aaagagaatc gcattattag gtgcatcttc actcggttta
     2641 atcacaatag tattacctgt tactaatgcc ggagccgctt tacgggcaat gaggaagaac
     2701 gggaagttcc aaggtaaaat gccggtggtg acgccaatcg cttttttgta taggaaaata
     2761 tgttcatttg ggcgatcact ttggataatt tccccttcat aacgtctcgc ccattctgcc
     2821 atatagtcta agtagtcagc ggtaaaatgt acctcaacgg ttgctaagga cagagtttta
     2881 cccatttctt cggaaatagt gcgagcaatt tcatcggcac gctcacgaat ccccacagca
     2941 atttgacgta aatattttcc tcgttcaatc gctggtaaac gttcccaagc ttcttgggcg
     3001 gcttcagcag catcgatagc gattttggca tcatcagctc cgccacgagg gacttcggag
     3061 atagcttttt cggtggcagg gttatagact gctaaagtgc gaccatctaa cgcaggcaca
     3121 aattcaccat taatatacat tttatgttgt ttcatcatga gttgctcctt ttttatccac
     3181 agtggaagtt gatgactatc attcggtaac tgaaccgcct ttgcaaacaa attgttttat
     3241 ttttgttaat ttatagatat aaatgtgaac aaaatcacat ttcttggcga taaaaaagca
     3301 acgaaaatgc ccgatttttc cataaatatg agaaatcggg cattatgact atcagtattt
     3361 ttttaacaga aattgcaaat ttttattaaa aaatcaccgc ttgtctattt aacaaagata
     3421 gaacttagta tgcgatccga tatacctcca acagctcctc agccgtacaa tctctcgggt
     3481 tgccaccggt gcagacatca ttaaaggcat cgaccgataa tgccgctaaa tcggcttctt
     3541 taacgccgat ttgatgcagt tttggcggaa tgccgacatc ttgtgaaagc tgttgtgcgg
     3601 cagcaatggc ggcgttgcgg tactcttctt ggctcatttc atctacgcct tttaccccca
     3661 ttgctcgggc aatttcacgg tatttttcgc cggtgtaatt tttattaaat tccatcacat
     3721 acggcagtaa tactgcgttg gcaatgccgt gtggggtgtc gtaataggca gaaagcgggt
     3781 gtgccatact gtgaaccgcg ccaagcccaa cgttggagaa gcccattcca gccacatatt
     3841 gtcccaatgc cattgcctcg acatctttcg gatttttctc cactgcacca cgcaatgaac
     3901 ggccgatcag ctcaatcgct ttaaggtgta gagcatcggt taattcccac gccgctttgg
     3961 taatgtagcc ttcaatggcg tgggtgagag cgtccatacc ggttgcggca gccaagcttt
     4021 tcggcataga ttccatcata tctggatcga ctaacgccac tgccggcaca tcgtgaacgt
     4081 ccacgcacac aaatttgcgt tttttcgctt catcggtaat gacatagtta atcgtgactt
     4141 ctgcggctgt acctgcggta gttggaatgg cgataatcgg cacacatttt tggtgggttg
     4201 gggcgacacc ttccaatgaa agtacatcgc taaactcagg gttattgatg ataataccaa
     4261 tcgctttggc actatcaatc ggtgagccgc caccaatcgc aattaaataa tcggcactgg
     4321 aggctttgaa ttttgcgacc cccgctttga tcacatcgac tgccgggttg gctttgacct
     4381 catcgaaaat ctcataaggc aagcctgcgg catcaagcaa ggcggtgact ttttgggcaa
     4441 cgttaaattt gatcaggtct ttgtctgtta ccacaagggc ttttttgaaa cttcggctgg
     4501 tcacttcgtg aacaatattt tggattgctc ctttgccgtg gtaactggtt tcattgagga
     4561 taagacgatt tgccatacta ttttccttct taaaagttaa acaattcgat taacttgcaa
     4621 gcggtcaaat tacgtcattt ttttgcaaat tttgataaaa atttcaccgc ttgttgcgat
     4681 tcttttgttg tgagtgtgct taggctaaat attcaaaata cgtttgattt catcacggta
     4741 ggcttcggca tcgctaccaa aaatagattg aataactgag cctgaggtca caacgcctaa
     4801 cgcaccgaca gatttaattt tatcccattc cacaatgtcg gtatttttca cctccacacg
     4861 taggcgggta atacaggcgt aaacatcgac aatattttct cgaccgccaa gtgcggcaat
     4921 caccactaag gctttatcgg taagggtatt tgacatattg gcttcctttc ggctgaaagg
     4981 gtgggcaacc ccaccccgtt taacttgaat gttagtaaag aacacctttc gcttcttcgc
     5041 tatcaatgtt ttctttcgtg acataaacca ccccggtatc aatcactttt tcgactttct
     5101 cgcctttaat cagcttgccg atggtgtcca cgcctttata gcccatttgg taggaagatt
     5161 gcaccacgat gccgtctagt gttccgtcac gcacgaagtt ttgtaggtcg gagtttccat
     5221 caaagcccac cgctacaatt ttgccggcaa agcctttttg tttcaccgct cgtgccattc
     5281 ctaccgcagt cggttcgttc gccccgaaga tcgctgcaag atcagggtta gaacctaata
     5341 cgtcttcggt ttggtttaac gctttggtca tatccgaatc ggagtagtac gggccgacca
     5401 gtttgtaatc ggcttttgct ttgatttcgt caataaaacc accggtacgc tcaatcgctg
     5461 atgctgcccc ttgggtaaag ctcatcactg ccactttgcc gcctttgact ttggcaagca
     5521 gtttttccgc tgctaattta cctgctgcac ggttgtcggt ggcaaggaaa gattggtagt
     5581 atttgccgtc actgttgatg ccggaatcga taagcaccac cggaatgcca ctctcataag
     5641 cttttttcac cgccggcact aatgctaccg ggtctgaggc agccagtacg atgcctgcca
     5701 cgccacggtt tacggcgttg tccaccatat tcacttgggc gtcaatcgct gtttcggctt
     5761 ccggcccttg gaaggtgact ttgtattgtc ctccaatatc tttgccggca gtttcagccc
     5821 ctttacgcac gttttgccag aaggtagagt tggcagattt cacaatcaca gcgatttcat
     5881 cagattttgc cattgcactg cctgcaaata aggtcgataa accaaagagg gtcgcagtaa
     5941 ataatgctga ttttttcatt ttaattctcc tttaagagtg gattattttt taccggcttt
     6001 tttggattgg cggagctggt cgagagagac cgccacgata atcacaagcc cgatgacaat
     6061 catttgaaca aatgaagaaa cgccattcat attcaaacca ttacggagta ccccgataat
     6121 gaatgacccg atgagtgtgc cggggatagt acccacaccg cccataagcg atgtgccgcc
     6181 caccacagca ctggcaatcg catccagctc gtaagagacc ccaccgtttg gctgggcggt
     6241 gacgaggcgg gaggcaagga taacgccagt taaaccagag agtaagccac tggcaacata
     6301 ggcatagatt ttgacaatat tggttttaat gccggataag cgtgcggctt cttcgttaga
     6361 accaattgcg taaagatagc gtccgacttt cagtttagag agagcgaaag tgaatagtac
     6421 ggtaataaag atcataataa tcacagggta aggaatacct gcaaatacca ctttcggcaa
     6481 gccgtttgcc ccttcttcca caattttaaa taatgctccg ttaccgagat tggcaaagga
     6541 ttcaggcatt ccggaaactg gtgcggcatt ggtaacatac aatgctaaac cacgggcaac
     6601 catcatcata ccgagggtag caataaacgg aggcaatttc attctggtga ctaaaatgcc
     6661 gttgattaag ccacaaatac cgccgaccaa aatccctaaa atcattccta gtggtacggg
     6721 tacaccggca tttacaatca ttgcagctgc cacaccggag agtgcaacta ctgaaccggt
     6781 acttaaatca attccaccgg ttaagatcac acaggttgcc ccaataccaa ttaacgcaat
     6841 ggtagaggtt tgtagcccta ctgtcataat gttattggag gtaaagaagt attcattggt
     6901 tacgctgaaa aagatgatta atagtactaa gccagccaat gcagccattt ttcttaacat
     6961 ttctttttgt ttttcattca tttttccacc ccatattcac gagcaaacat atttttaaca
     7021 ccagttgcat aatgcataat ttcttcttgg ttggtttttt tagtctcgag catacctgtc
     7081 attctacctt cacgcattac aatgatgcgg tcggaaactc ccaagacttc aggcaattcg
     7141 gaagagatca cgataacacc cacgccattg gcagcaagct catcgagcag ttgatagata
     7201 gcatattttg ctccgacatc aattccccga gtcggctcat caaaaatcat cacttttgct
     7261 tcacggaata accatttacc aattaccact ttttgttggt taccaccact taagttttga
     7321 actttctgct caatcgttgg ggttttgatt tccattttgt cgatgaaagt ttttgaggct
     7381 ttttcttctt catcgtaaga aatcacacct atttgattgg caactttatc catagatgcc
     7441 atcgtgatat tctcacgaat cgacattctt actgcaacac cattgagctt acggtcttcc
     7501 gacaaatagg ctataccggc tttaattgca tcactcggat ctttaatgct gactttgttc
     7561 tcgtgcacaa aaatctcacc gctatcaagt gggtctgctc cgaagatggc tcgagccagt
     7621 tcagtacgtt ttgctccaac caagccggta atgcccagaa tttctccttt gcgtaagtca
     7681 aagttcaatg gttcaaacac tcccttacgt tcagcattca taatcgagag gataatgtcg
     7741 tttgtcacca cagcggtttt tggtgggaaa tggttatcca atgaacgccc aaccattttg
     7801 gtaatgatct catccatcgt gatttcattg aatttatggt tagatacata ggtgccatca
     7861 cggaaaatcg taatatggtt acagatacgt tttaattctt ccattcggtg agaaatatag
     7921 acaatgctca cccctttggc acgcagtcta tcgaccaatt caaataattt atcgatctct
     7981 ttttcggtaa gagcggaggt cggctcgtcc ataatcagta cttccacatc aaatgaaagg
     8041 gctttggcaa tttctaccat ctgttgtttg gctaccgaca acattctcac ttcatcgttt
     8101 ggatcaacat caatctcaaa gaaattcagc aatttagccg attcttccac cattttgtct
     8161 ttatccacta aaccgtgttt gagtggctca cgggcgaaga aaatattctc tgccaccgtt
     8221 aaatggggaa tgaggttcag ttcttggaaa atcatactaa tgcccgcttt ttgtgcctct
     8281 aataccgact caaagcgaac aggctgacct ttatatatga tttcaccttc atcggcttga
     8341 tatacgccca gcaagcattt catcaaggtg gatttacctg caccgttttc gcccattaat
     8401 gcgtgaactt cgcccttacg cagcgtaagc tcaacacctt ttaacgcatt gacccccgca
     8461 aaggatttac tgatgttttt catcgtgagt attacttcat tttgcatact gcactccgtt
     8521 tgtaaaaatt ttaagaaata tcaccgcttg tatgttattt atggcaggct attgttcgct
     8581 ttccacccaa gaaccatagg acttaaattt tccgagtacg atatccattt gttctttgtt
     8641 tagcgttggg ataggttttc ctgtagcgag cagtttcaaa taccattctg cgatcacttc
     8701 cacctcgtgg gcgatgccga gggctttttc gagggtttta tctgcggtga ttaagccgtg
     8761 gtgagcgagt aaaattgctt tgctgtgttt aatgccttca cgtacatagt cggctaattg
     8821 gtgagtgccg aacggggcat aaggtacaca aggaatgtga ttagtgccgg tgcaggcgat
     8881 catatagtga atggcaggga taggttcacc taaaatagac actgcggcac agtttttagc
     8941 gtggttgtga actaccgcat ttaattcagg gcgagcctca taaaccgcta aatggaactg
     9001 ccactcactt gaagggagtt ttccctcttc gtgtttgccg ttgccatcaa caaagacgat
     9061 agaatcttcg gtcatttgtt cataaggtgt acctgttggc gtaatgagca taccgtcttt
     9121 ataacgtacg ctcacattgc ctgctgtacc ttgatttaac ccaattcggg tcatttcaag
     9181 acaagtatca atgatttgtc tcgccagtat ttttctgtcc attgttgaac tccttttaat
     9241 tttatatttg taaaaaattc tgctttttta accgcttgta attaaaccgg tgttacgcct
     9301 tttttgataa taatgttgcc gtatttggct aactcgcctg taatgacaac ggcataagcg
     9361 tttttggctc tatcatagaa agcgaagcgt tctacacgct ctattttcgg ggctgttccg
     9421 tttacttttt taattgcatt gaggtaacgc tcttcaacgc ttggatcgag cgtgtcgcct
     9481 tttactgctt gcatcattgc aagtggggct tcaacgtatt ggtcaaattc aaacagagca
     9541 gaaatgcctt ctaataaggt ggcaacactt aagccatctg cacgaatgac gttttgatga
     9601 atagagtgag cagggaagtg tgcatcagaa agcactaatt catcgccgtg ccccatttcg
     9661 gctaagactt ttaataaatc tggagaaatt gccgggtgaa taccttttaa cattttgaac
     9721 tcctttttta ccctctctct gatttgaggt tctgaatgaa cctcaaatca gagagagggg
     9781 agaaacttta tttataaaat tgactatttg acgatggctc aacccgtttc acatttggct
     9841 tcattgcctg ttgagccgca tttggatttt caaatacacc agcacctgta aaagtaaaca
     9901 ttgccgcgcc taatacggta ctttcaggga aatccactac atcaatcggt ctatttaaga
     9961 catcggctcg aatttgattc cataaagcgt ttttagaacc acctcccaca caaatcaaac
    10021 tctcagcatt aaaatgaccg acttcttgta atacatctaa cccttctttt agacgatgag
    10081 ccatatattc caagcctgca cggtaaattt cgccacgagt gatgtgcata gataagccga
    10141 taattgaacc tgttttagct gttgtgccat caaaattgcc gactaatttc acaccgtttg
    10201 agccagcagg cacttctgcc gcttcttcaa tcattgtgcg gtagtaatgt tcagtattgg
    10261 ctatatcaga gaagaaacgt ttgccaaacc actccatcac acctgaagct acccactgca
    10321 cacctgggtt gaaataacca gattggctat caaactcaat ggttaagccg ttttgaacga
    10381 attgccattg tggttttgct tttggggtac gaaccattaa aatttcccaa gtgcctgaac
    10441 ttaacacagg ttggttatag ccagctcctg agccaaaaat ggcaaattga gtatcgtgtc
    10501 cgcaagaaat tactggaatt tgaccgctta aacctaactc ttgggaaatt tctcctttta
    10561 atgttcccac aacttctcct gcacttttca ttggaggaaa atggttttcg gttaagccta
    10621 ataaatctaa aactgtttta tcccacgaat cgttttcgat gtttgtcatc attgacgttc
    10681 ccgccattgt gcggtcggtt gtttgcacgc ctgtaagacg gtaagtcaaa atagatgaga
    10741 tgaataagaa actctcagct tgttgataaa tttctggttt gttctctttt aaccacaata
    10801 atttgaataa ggtgttgaaa ctataatgtc caacgccatt acgttgatat aacgcctcaa
    10861 catcaagata attggcaagg ttttccataa tcggtagggt tctcgggcat ttccaagaaa
    10921 tgatcggata aagctgcttg ccgtttttgt caaataatgc accgtccacg ccaaaggtgg
    10981 ttactgaaat cccaacaatc tcttgtcgtt gttgttctga aagttgagaa agcgtctgtt
    11041 tggcacaggt cattaatttc gaccagatct cttcaatatc ccaaatatga aattctgggt
    11101 tttccacatc tggttgtgtg ttattcggta gatgatgggc tgccacaatt tcacccgctt
    11161 gattaatcgc aatcgtacgg aggttggtgg caccacagtc gaaaataaga gaaattggca
    11221 ttgttatctc ctttacttat cctaatatct cccttttaga gagatagatt aaattgcgtt
    11281 tagcaattta atcagagaga gggatttgta aattttgaga gaaaagcgac cgcttgtagc
    11341 cctctccctg ccattcaatt ctgaacgaat tgcttggctg tccctctccc acaaggggag
    11401 agggtgattg tgtattattt atataatggt ccgaagttag cacaagcacg gtagtcttga
    11461 ccttctttgt cttgaccgaa accgttccac gcacttgggc ggaatacgtc tttatcttcc
    11521 acattgtgca tacaaaccgg aatgcggagc attgaggcga gggtaattaa gtctgcccca
    11581 acgtgtccga aggttgccac acagtggttt gcaccccagt tcgccattac gctatacaca
    11641 tcggtaaatg cgccttttcc ggttaagcga ggtacgaacc aagtggtcgg ccaggtttca
    11701 ttggtgcgtt tgtttaaaat gtcgtgtaca ttttccggca agtcgataga ccaaccttcg
    11761 gcaatttgta aaaccggccc gatacctttg atcaggttga tacggtgcat cgtaaacggc
    11821 attccacctt tggttaagaa ttgggaagat aaaccaccac cacggaaata ttcgtgaact
    11881 gccggacacc aacaggtgtt ttctaaacaa cgtttaccgt cttcttcagt cacttcccaa
    11941 gccggtttaa tagttggttt gcctgctcta tcggtatgct gacctgttcc gtctaacgcc
    12001 gcagagccgg agttgattaa gtggataaaa ccgctttccg gacgccagcc ggtgacacgt
    12061 tctacctggt cttcgctcca gtatgtccgc acatcggcaa agatttgtgc ctgaccggtt
    12121 aataagttac cgaacaacat attcacgcca tttaagctgt cgttttcagt tgccataatg
    12181 tatggtgcgc gaacgccgtt ccagtcatag gtcgaattca aggtagcttc cataaaatcg
    12241 ccgtttggca aatggtctgt ccattggcgt tgcccttgga aaccggcagc aatcgcatta
    12301 tgccctaagg cttcttcact atagcctagc tctgctaagc gaggattacc caccattaaa
    12361 tcacgggcaa taatggtcat tttcaccacg tcttcccaga gtttttcacg ctcttccggg
    12421 ctgcgttgat tttccggtgc gttgacatca ataccttctt tgcaataggt tttcacccag
    12481 ctcattgcta atttaaattc ttcggcatcg taaatatggc gatctaaacg gcgtttaatt
    12541 tcggtcatat ccacatattc gttacgcata cccaaatatt cttggaagaa ttgttggttt
    12601 acgatagaac cggcaatacc cattgaaaca gagccgatag ataaatatga ttttcctcgt
    12661 aaggttgcca ctgctaagcc ggcacgggca aagcggagta atttttcacg cacatcgtct
    12721 ggaatagatt gatcgtcagc ttcttgtact tctgtgccat aaatggagaa tgccggtaaa
    12781 cctaattggc tatgacctgc taaagcagca gctaaataaa ctgcacctgg gcgttctgtt
    12841 ccgttaaagc cccaaatggc tttcggcata tgtgggtcca tatcaatggt ttcagaaccg
    12901 tagcaccagc aaggggttac ggtaatcacc gctccgacat tttcacgttt gaatttttct
    12961 gcacaagccg cagcttcggc aacaccaccg atggtggaat cagcaatcac acattcaacg
    13021 aaatcaccat ttgggtggcg gacttcagat tgaattaatt gtgcaacggc ttttgccata
    13081 ttcattgttt gatcttcaag tgattcacgc actcctaaac ggcgaccgtc aatggttggg
    13141 cggataccaa tttttacagg ggtagatttc aacgttgtca taaatagttc tcccgattgg
    13201 ttaaaaaaat ttgcgattga atttattatg catacgcaat atgagttatg aagaaaaatg
    13261 cgaatttagt ttaaattttg tgagtgcgat cacaaaaaat gggggatttt ggtaaaaatg
    13321 agtgaaaaaa gaatgcccga tttgttttat ttctgaataa aatcgggcag aaatcactaa
    13381 ataatgattt caatttgatg tgctaagcaa taatttttga gaaagtccgg tgggggttgg
    13441 ttgataaata atttatcaat gcaagataaa tcttcgatac gaaacaagtc tttacgctca
    13501 aatttactgt gatcggctaa taaaaaaacc tgtttactgt tcgcaattaa tttacgttta
    13561 agggaaatat taatttcatt tgattcccaa atcgccccat cggcaatgcc tgtacaagaa
    13621 agaatggcaa gatcaatttt taaacgggtc agtaactgct cggataatgg accatagaag
    13681 gcatcgtatt tttcggaata aataccaccg gtggcaatcg ttttaatgtg cggtttgtgt
    13741 gaaaggctgt agataatccg cattgaactg ctgacaacgg tgcaaggaat atttggaata
    13801 cgctgtgcca cattccaaga ggttgagctg gcatctaaag caatcaccga ttctggttgt
    13861 aaatattcca acacattttc tgcgatactg cgtttcgcct cactgttcag ccgttgtcgg
    13921 gtggtaaatg agcgaccaat atctcgcttt ttatggctca cagctccacc gtgagtgcga
    13981 tgtaataatc catctttatc tagcttattt aaatctcggc ggatagtttc aatcgaaacc
    14041 ccacaatgtt ccgccaattc ttgaacgtta gcttcatcaa attgggcgag ataagcgatg
    14101 attttatctt gtcgttgctg cataaatagt ccttagtaaa tttagacata aatttaactg
    14161 cttgtaggtc ggtctgtcga gtggataaat cggattttta gagcgtgatc acatttgttt
    14221 tacaagcggt catatttaga gagtttttta caaaatctac cttattttgt gaaatttaat
    14281 ttcagactaa ttgacaacca attttaactc tgtaaaattc gccttgtttt gatgttgaga
    14341 attgttttta atatcaaaat tttggtaatc ctcttaaata atttacctaa ggaaataaac
    14401 agatgaaaaa cttcaaatta cttccggttt ctgctgcggt attaagcgtg cttgcagcga
    14461 acgcatttgc tgaaacagta gcaacggatg cagcggtttt agatacggtt acagtaactg
    14521 acaaccaagg tttaaaagta caaaccaatg tagttacaac tcagaaaaaa gaagaaagta
    14581 ctcaaacaga cttacgtggt ttattaaaag atgagccggc aattagctta ggtggtggta
    14641 acggtacatc acaatatctc tatattcgtg gtatgggtca aaactcaatt gatgtaaaag
    14701 tagataacac ttactcagat agccaaattt tgtatcacca aggtcgccat atgttagatc
    14761 ctgcacttgt aggtacggtt acagtacaaa aaggtgcggg tagtgcttca gccggtattg
    14821 gtcaaactaa tggtgcagta attgctaaaa ccgttgatgc attagattta ttaaaaaaca
    14881 gcgataaaaa cttcggtgca aaattaggta ctggcttcag cacaaaccac gctcacaatt
    14941 tcaatgcaac cttatatggt aaaggcgaaa tctttgatgc tttagtctca ggtaatcttg
    15001 tgagagatcg cgactataaa ggcggtaaag attacgtaaa ccaattcggc acaaaccgtg
    15061 taccatacag tgcgttagat aaaagtagct tcttagcgaa attaggtgca actcttggtg
    15121 atcatcgctt tgtattaagt cattctaatg agcgaaatga aggggagcgt ttggttcgtg
    15181 aagagtttga tgcagcacct gtaggtacta aaggtagccg tgtaacttta gatcgtcaag
    15241 caccggcaaa tcgtagaatg acagtacaaa gaactaactt agagtggact ggtaaaaact
    15301 taggttttgc tcaagaagca acagctaacg tgtatcagtt agtacaaggt cgttggtctg
    15361 agaatgaaac acgtaatggt tatgctggtg gtgtagatta tgcaaccaaa actaaaatag
    15421 taacccacgg tgcaaacgta aactttgatt ctgcccttca cgaaaatgta ttattaaaat
    15481 acggtgtgaa ctatcgccac caagagacta aaccatatgc taaatttagt catcaattag
    15541 tgaaccaaga gaaaaccgac accggtgtat atcttgaagc gattactgct ccggttgata
    15601 aagtgacttt aaccactggt gtacgttacg accacttcaa attcaaagca atggacggta
    15661 aaaaacgtag tgagggtgca tttaacccaa gtgtaggcat tatttatgag ccaattcagc
    15721 atttaagttt tagtgcaagc cataactacg caactcgtag ccctcgtatg cacgatgcgt
    15781 tactatctca cggacgtcgt ggtgttgtat caattgctaa caatactaaa gcagaacaag
    15841 ctcgtagtac ggagattggt tttaactata atgacggcac cttcagcttt gacggtagct
    15901 atttctggca aaacatcaaa gatgctttag gtacatcaac cggtcgtaac aaccatctat
    15961 gtcctgaact gacacctaat actaataata aagagtgcta ctcagaaatt atcaatgcag
    16021 gtactattaa aaaccgtggt tacgagttaa acgcaggcta ccacaacaac ggttttactg
    16081 ctcgtgttgg tgttgcacat agcaagcctc gtttccaagg tgatagatta agtactaacc
    16141 ctgagtatgc atctgcaatc ggtcgtactt ggacagcatc tttaagctat cgctttgacc
    16201 aaccaaactt agaagttgga gtacatcacc gcattattga aaaagttaaa gctgaagata
    16261 actactttgt tatgaataat aatggtattc ctcaatttgg acgtggtggt ccaacaggta
    16321 aagacggtta taacgtaact gatattacct taaactggaa accgttgaat gatgatagcg
    16381 tgaatgtaaa ctttgctgta gataacgtag caaataaaaa atacaacgca cacggtcagc
    16441 gtggtcaatt atcagctcgt ggacgtgaat tccgtgcagg tgtgaactac acattctaat
    16501 ctaatcccat ttagtcaaaa cgcccgaatt atcgggcgtt ttttatggct tacaagcggt
    16561 tatatttcag tgatttttgc aaataaaaaa ccgactattt tagtcgtttg ttatttaaat
    16621 gcgtggataa agtctgctac tatgaataat ggtaataatc cacacttcat tttctaattc
    16681 ggtataaaca atactataac gcctagtgaa tgcttctctt gtgccatctt ctcttaaacg
    16741 tccagccgca gagggcatat aagcgatagt ctcaaatttt tggtaaatat cttcttgtaa
    16801 gcgaattcca ctactttcaa aaccagtata ttcaattacg ctcgctaaaa tttcttcaat
    16861 atcattttga gctctactgc taattcgtaa tgttttagcc ataaatgata ttctcttgtt
    16921 caaagtttgc gagttctctt gctttacgtt caatcagttg tttggttcgt gcttttgatt
    16981 catcgagtga aagaccatta cctgattgca tatcttctaa gccaaggcga attttttctg
    17041 ctaggaattc atcatagcct ttctctttaa acataatctg ctcctattaa gatataatta
    17101 aaagcattgt ctgcttttaa ttttgagttg tcaaattaac ttatttttaa attatctttt
    17161 aaaacagatt gatagagggg accttctttg aaaggtattc attagtattg aacaaattta
    17221 tttgatacta atgaataaat gtatctaaca gcaaacgtct gacaaatata gcaatcgttt
    17281 gcgcaatcgt ttataatcgg tttagttttt catcttaaat aggaaaatcc aaagaatgac
    17341 aatccaaacc ttccgtggct cgcctgcctt atctgctttt cgcattcaac aacttctgca
    17401 aaaattccaa caaaatcaat tacctgtaaa atcagtttat gcggagtttt tgcattttgt
    17461 ggcattaaat cgaccgcttg tagccgatga agaaaacaaa atcaaagagt tattgcacta
    17521 cggtccaacg ctggaagaag acgagccgac agggttttgc ctgattgtta cccctcgtat
    17581 cggcacgatt tcctcgtggt cgtctaaggc aaccgacatc gcccataact gcggtttggc
    17641 agcagtagag cgaattgagc gtggcacggc atattattta gaattttctg aaacgccgaa
    17701 cgaaagtcaa cttacgacgt tgaaaggctt attacacgac cgtatgatgg aaacggtgct
    17761 ggataacgaa agccaagccg aacagctttt cgctcagcaa gagccgaagc cgtttacttc
    17821 ggtggatatt ttaggcggtg gtcgtgaggc tcttgaagag gcgaacgtga atttaggttt
    17881 ggcattggcg gaagatgaaa ttgattattt ggtagaaaat ttcaccgcac ttggacgcaa
    17941 cccgaacgat attgaactgt atatgttcgc ccaagccaac tccgagcact gtcgccacaa
    18001 aatttttaac gcagactgga cgattgacgg cgtgaaacag gaaaaatccc tgttcaaaat
    18061 gatcaaaaac accttcgaga aaaccccaga ctatgtgctt tccgcttata aagacaatgc
    18121 cgccgtgatg gaaggttcaa cggtcggccg tttctttcca gatcaagacg ggcaatatcg
    18181 ttaccatcaa gaagatgcgc atattttaat gaaagtggaa acccataatc atccgactgc
    18241 tatctcgcca ttccctggtg cagcgacagg ttcgggcggt gaaattcgtg atgaaggggc
    18301 aactgggcgt ggagcgaagc cgaaagcagg tttgacaggc ttctcggtct caaatttggt
    18361 tattccaaac tttgaacagc cgtgggaaaa cccgctctct aaaccgagcc gtattgcttc
    18421 tgcgttagat attatgatcg aaggcccact tggcggtgct gcatttaata atgagttcgg
    18481 tcgcccagca ttgcttggct acttccgcac ttacgaagaa aaagtaaata gttttgcagg
    18541 tgaggaagtg cggggttacc ataaaccaat tatgcttgct ggcggtattg gcaacattcg
    18601 tgctgaacac gtgcaaaaag gcgaaatccc agtgggagca aaattaattg tgcttggcgg
    18661 ccctgcgatg aatattggct tgggtggcgg tgcggcttcg tctatggctt cgggcaaatc
    18721 gaaagagaat ttggattttg cctcggttca gcgtgaaaac ccagaaatgg aacgtcgctg
    18781 ccaagaagtg atcgaccgtt gctggcagat gggggcggag aacccaattt tatttattca
    18841 cgacgtgggg gctggcggtt tatcaaatgc gatgcctgaa ttggttcacg acggcgaacg
    18901 tggcggtaag tttgatttgc gtaacatctt gtgcgatgaa aaaggaatgt cgccacttga
    18961 aatttggtgt aacgaatcgc aagagcgtta tgtgcttgcc gtttcccctg aaaaactcga
    19021 cattttcaca gcattgtgcg aaagggaaag agccccattt gcggtgattg gcgaggcaac
    19081 gaaagagaag catttaaccc ttgctgacac acatttcaac aataagccga tcgatttgcc
    19141 gatgaatgta ctgcttggta aaaccccgaa aatgcaacga gatgcttcgt caaaaaccgt
    19201 aaaaaatcca ccgcttgtca ccgctgatat tgatcccaaa gaggcatttc atcgtgtatt
    19261 acgtttgcct gcggtggcgg aaaaaacctt cctgattact atcggagacc gttcggtaac
    19321 cggtatggtt gcacaagatc agatggtcgg tccgtggcaa attccagtgg cggattgtgc
    19381 tgtcaccacc gcaagtttag acagctatca cggtgaagca atgtcaattg gcgagcgatc
    19441 acctgttgct ttgcttgact ttgctgcctc agctcgcctc gccgtggcag aaagcattac
    19501 taacattgct gcaaccaaca ttggtgatat taaacggatt aaactttcag caaactggat
    19561 gtcagcagca ggtcacgagg gggaagatgc agggctttat gaagccgtaa aagccgtggg
    19621 cgaagagctt tgccctgcac ttggcattac aatcccagtg ggtaaagact caatgtcgat
    19681 gcgaaccact tgggaagaga acggcgaaca aaaatcggta actgctcctc tttctctcgt
    19741 gatttccgca ttcgcccgtg tggaagatgt gcgtaaaacc gttaccccac aacttcgtac
    19801 cgacaaaggc gacactcgct tgctgttaat cgacttaggc gaaggtaaca accgcttagg
    19861 tgcgaccgct ctggctcaag tgtataaaca gatcggcgac aaaccagctg atgtggtcaa
    19921 tgttgaacgc ttaaaaggct tcttcaatgc aatacaaatt cttgtcgcac aagaaaaact
    19981 actcgcctac cacgataaat cagatggcgg tttaattacc actcttgcgg aaatggcctt
    20041 tgccggaaat tgcaaaattc gtgctgaaat cgaccgcttg ggaacagacg acttaggcgt
    20101 gctgttcaac gaagaacttg gggcagttat tcaagttaaa acctctgatt tagagagcgt
    20161 acaagcggta ttaaaagcgg aaaatcttgc aaatcttacc tacgatatcg gttcagtcgc
    20221 acagggtgat ggctttgaaa ttacccgaaa tggcgaaatg gtattcagcc aaaaacgctc
    20281 cgaactgcga ggcatttggg cggaattgac ccaccaaatg cagcgtttgc gtgataatcc
    20341 tgaatgtgca gaccaagagt ttgaagcgaa aaaagacccg aataacaaag gcttatcggc
    20401 gtttttaacc tatgatgtga atgaagacat tgccctgcct ttcatcaaca aaggcacgaa
    20461 accaaccatc gctatcttgc gtgagcaagg cgtaaacagc cactacgaaa tggcggcagc
    20521 gttcgaccgt gctggtttta atgcaattga cttgcatatg tccgacttga tctctggtag
    20581 acgtaaccta aacgacttca acgcaatggt ggcgtgcggt ggtttttctt acggcgacgt
    20641 actgggtgcg ggcggcggct gggcgaaatc aatcctattc aacccaatgt tgcgtgatca
    20701 attcagccaa ttcttcgtta acccgaacac gctggcatta ggggtatgta atggttgcca
    20761 aatggtctcc aatctagcgg aaattattcc aggcacagaa aactggccac gcttcgtgcg
    20821 taataaatcc gaacgttttg aagctcgtgc cgcaatggtg aaaatcaacg acaccaactc
    20881 gctctggttc aatggaatag ctggcagcca tatgccgatt gcagtcagcc acggtgaagg
    20941 acaggtggaa ttcaagcggt cggaacagct cgaaaacttg caaaaacaga acttggtcat
    21001 tgcccaatat gtcgatagcc atctcaaccc aaccgaacaa tacccagcca acccgaacgg
    21061 atcggcgttg ggtatcaccg ctatcggtaa tacagacgga cgagtcgcta ttatgatgcc
    21121 acaccctgaa cgtgtgttcc gtgcggtcag taactcttgg tgcccagaag attggacgga
    21181 agatggagcg tggatgcggt tgtttagaaa tgcgagagtg gcgttgaagt attaaaaatc
    21241 taccttcgta gtgatctgcc cccatagggg gcgattaaaa ttcgaattca tagaacccga
    21301 atttttatat atctgataaa cgaagttgtt tatcctctaa ttcgtgcttt tcctgatatt
    21361 tcaagtactt tcttatcatt tctgcattga catctacggt gtctacgcag tagcctcttg
    21421 ccacaaaagt gggttgcccc acaatttatg cttacgtaaa tagggaaact tattgaaaaa
    21481 tcttattgga ccctgttcac ccttttaaat gccccattac gtcttataca gaaaatgaac
    21541 gtgatttatt tgaacggtca attctacctt aatagaaagt ggtttttagt tagcaatcaa
    21601 aatcacatta acggaattga gaatttggag agtcaggcaa aatgctattt gcgtaaattt
    21661 aacggcgttc ctaaagtaca tgaattctat ttgaaagaat gcagatggcg ttttaatctc
    21721 agtaacttaa aatatcaagt tttcttttta aaacaattgg ttaaattgag tttctaggat
    21781 agccccttat ttttataatc ctctatttaa gtgcatattt ctatttatgc taatcgtcta
    21841 acgtatcctt ttattataat attgaacatg attactattt tcgtcttgtg ctatactaga
    21901 ctcgcgcaat aaaggcacac ttctttcttc agtgtcttta gcgttgcgat ttaaatttaa
    21961 ttacattttt tataggaaag acaatagaaa tgaagatcaa aaaatatgta tttcacctga
    22021 cttgtacaac acttatctgc cccttaccta gggtattcgc agaggaaggt tatatgttag
    22081 atacggttga ggttaaagcc caagcaataa aaaaaacaga tgaagttttc attaaaacag
    22141 gggcaataag cacccgagat aaaatcttgg aatcaaacca aagcttagag actattgttc
    22201 gttctatgcc tggcgcattt acacaaatgg ataaatctca aggtgtggtt tctgttaata
    22261 ttcgaggagg ttctggattt gggcgagcta ataatgcaat aggcccaaat tatatgggaa
    22321 tgctcgctgg caaataccaa ttcaatgact ctacttggat aggagcatta tttgggcata
    22381 gttggcgcaa aatctctcaa aattataaaa taggaggcgg acgaaaagtt acggatagct
    22441 cgattgacct tagtaaaatt gatgatctag ataaagaaag ttctaccacc tcaccgttca
    22501 atgcagctca tttaaaacaa aaaccaatga gtcgtatggc taaattggaa tatggcgata
    22561 aataccaaac catcacacta ttaccaaagt caggtagcgg gtagacattt aaaaaaccaa
    22621 aacaaccagt taaattatcg ttttcacttg cccgagtcac cttggttaga tgttcaagtg
    22681 gcctatgcgg aaaatcaagg caaacaaaac tacaacaaag ggagctgaat taataataaa
    22741 tatttaattc aaggtttggc aattaacaac aaagcgacaa ccttaaattt gagtaacacc
    22801 tttaaagtgt ctccgtttaa agatggagaa tgggaagcaa tattaggttt taatcgttta
    22861 aagaaccgct atagtaaaaa tagacatcct gcggaattaa actataactt agagaatggc
    22921 gaaaccagcg aaaacaacga ttctgtagga atgacagccg ttcgtaaatc gctttatact
    22981 tcaactttcc agcctgatgg cgaacagaag ttcgatactt tttatctcga caatcacttt
    23041 aactatggta tatggggatt ggatataaac gcaaatatta ataccgctcg gtttattggt
    23101 cagcgtttta aatattttcc ttttgcgatt agagaattgg agtcagatat tagacaggca
    23161 agtagaaata ggcagtttga gcatttaagg tcattacaat ctcaattaga caacttgcaa
    23221 aaaaacattg tacatatatt ccagatgagg attttcctaa tgatcctgaa ttgggagagt
    23281 ttaactgtcc tgatgtcaat tttcctcata cagcaacagg aaaacataaa cgaattaatt
    23341 attcagcaac cttgtctgct tatattcacg atttattcac gccctttatt agttattctc
    23401 aaacacatcg agcccctaat attaaagaaa tattcttctc taatttaggt gactatggtg
    23461 taaataccgc actacaacca gagcaagcga aaacaacaca gtttggtatt aatggattca
    23521 aagaacgggt atttagtgaa gaagataaat taggatttaa agtgctattt tataacactc
    23581 acttaaaaaa ctatatttat aatgtacaac ataccgctgt tggtagtgct cctagtttat
    23641 tcattttcca caaaaactat gaaaaaccag taaatatcaa agggatagaa ttagaattta
    23701 gttatgatat aggctctttc tatggcaact tagcttatgc tcgtcaaaat acgaatcaac
    23761 ccgtatcctt tacagatgca agttcaaggg tagatgccgc ttctcattat ggttttaaaa
    23821 ctcaaggttt tggtgccagc aaaatttcag cgttaccaag agactatggc tcattggagt
    23881 tagggacaag atggtttgat caaagactac aaattggcgg agtggcaaaa tattacggaa
    23941 aaagtaaacg tattgcgata gatgagttta actatactta ttatcctgga acacatattg
    24001 tgaaagaaac tcatcggctt gaagaaactg taaataaaca acctattatt gttgattttt
    24061 acgtgagcta cgaaccaatc aagtcgctta tattaaagtt agaactataa aatgtctttg
    24121 acaaaaagta cattgatcct cttgatgcta ataatgatgc agcaagtcag agggtttaca
    24181 attctgatat cagtgatgaa cctatttata cgctcaataa ttatgcaaga gggagaacta
    24241 tagtgtttag cacaagttat aaattctaaa tggggttgtc ctagataagc tagcttaaat
    24301 ttcctttaac taattatttg gtatagtgac taaaattgag tctgcacagt aggttaaaac
    24361 cttataatat aaggatttaa cccagatggt gcaagcgttc acgcttgtgc cagttaattc
    24421 ctgttctgaa agtatttcag tcataagata aaagcctaaa ttagccaagt aaaatttggt
    24481 ttatttgggc ttttgttttt ggcacaagcg tggacgcttg cgtcatcatt ttatttatcc
    24541 atccattgct atcatttcag tatttctttg gcttatcaca agcggttaaa ttttatcaac
    24601 actttgcaag ttttaatgat ttttgcgatc tagatcgaaa aagttatatt ggcttgtgga
    24661 atttaatttt gaatagggta tttttcttat tcctttttaa gttgagaaag gctatgagcc
    24721 gaaaatataa attccacgaa aagagcggtg cttattttgt cagttttgca actgtttatt
    24781 gggttggtgt gtttgtgaga gagatttatt ttcaggtaat tgttgatgcc ttagattact
    24841 gcagaaaaca taagggaatg attttgtatg cctattgtat tatgccaact catatccatt
    24901 tattatttca agcagaaaat caaaatcctt ctgatttaat acgagatttc aaaacattca
    24961 ccgctaaaat acttattcag agaattaaag ataatttgca agaaaaccgt cgggaatggt
    25021 tgctgtggat gttttctcgg gcggctgaac gacgttgtaa tgtaaaacaa tatcaattct
    25081 ggcaacatca taaccaacct attgaaatct attcgcagaa attctttgat gaaaagttaa
    25141 attatattca tcaaaatcca gtggtaagtg gttttgtgtc tgagccttgg gaatggaaat
    25201 ataattgaaa taaactgctt gtatttttgc gatctctgtc gaaaaattgt gaatttttac
    25261 ttgtctgtcg gagaattttg cgtaaattct gcttattatg ttttagttaa aggtattcta
    25321 tgagtagata acccaaatga aaatagcgat ataacctatt tctacccagt aattaattcc
    25381 cgcttttatg tatcagatca aataatttac actgatttat cggtattatc tgtctttagt
    25441 ttttttaatt aaggagcctt atatgaaatt caaaaaatta gcattagcag caatgttagg
    25501 tggcttattt gtaacagcta atgcggcaga attacctaat atcactattt tagcgacagg
    25561 cggaacgatt gctggtagtc agcagaatgc aaatagtagt gcttatcaag cagggcaatt
    25621 gaatatcgaa accttaattg aagcagtgcc agaaatgaaa acattggcga atatcaaagg
    25681 tgagcagatt gtgaaaattg gcccactaaa gccttgttag atgtaggcta tcaaggtatt
    25741 gtcgtagcag gtgttggtaa cggcaatatg aatgcggaaa atcttgcatt atttagagga
    25801 agccaacaat atttttaaga tttttaataa acgttaaagc atataaaaaa tggcataagt
    25861 gagtcactta tgccattatt tttggggctg tcctagataa ctaacctaac ccccctttaa
    25921 ccaattgttt taaaagg
//
LOCUS       DANKBV010000021        25132 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.021, whole genome shotgun sequence.
ACCESSION   DANKBV010000021 DANKBV010000000
VERSION     DANKBV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25132
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.021"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            109..690
                     /locus_tag="PWY43_000676"
     CDS             109..690
                     /locus_tag="PWY43_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2135577.1"
                     /translation="MSQPALRQISPSQLEDIRLQMVKFAFLQLKNNELAEDVVQEAFV
                     SAYKYADSFKGESALKTWIFAILKNKIVDLVKAKGKLVAVSELMEEDDHDLADKLFNE
                     NGGWDKDAYQSNEWKNTESHTYSAQFWQVFEFCLNNLPANQARVFMMRSHLEMETEEI
                     CAECEISPANVHTLLYRARLQLQVCLSQKWFGG"
     gene            691..861
                     /locus_tag="PWY43_000677"
     CDS             691..861
                     /locus_tag="PWY43_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL2135578.1"
                     /translation="MLKCKQITELVSLSQEQKLTLKQRVNVQVHLMLCSRCRAFSQNC
                     KTVDKLTKEFNS"
     gene            complement(910..2127)
                     /locus_tag="PWY43_000678"
     CDS             complement(910..2127)
                     /locus_tag="PWY43_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL2135579.1"
                     /translation="MGASLIGALTITQAIIAMSFGCIVIAIGLVLIGNGGHKYGIPFP
                     IQLRSSFGTTGVKIPGILRGVPAIVWFGFQSWVGAGAINMCLKLLFGFDNLPVVYVLF
                     TVLQVGLSINGFKGIKWLENVSCVFLIGILAYMLYVVNTKFSVEIGERFANIEGTWGM
                     PFWAVTTAFLGIYSTMILNASDYSRHVKKGTTPTATGVIYVLSILPVTLFMGLIGLLV
                     TAATGNSDPIEVFATAMDNKFLTVITLLFIAFAQVTTNVLNNIVPPSYILMDSFKMKW
                     SHATILVGILSICVMPWKLVTAESADGLSLFIKIYSAFLGPIFAVMVVDYYILRKRTL
                     NVNDLYNKEGIFKGINWAAIIAITIGSLCSLIVVDLSWYVSLLPSGLSYYFLMKVLKS
                     SASFRKGTVLEHQ"
     gene            complement(2112..2267)
                     /locus_tag="PWY43_000679"
     CDS             complement(2112..2267)
                     /locus_tag="PWY43_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135580.1"
                     /translation="MQHNTQLNPELAKEVNVDLQPTKERIMDNFSYWASFLVAVFLLV
                     HFLWGQA"
     gene            complement(2270..3649)
                     /gene="allB"
                     /locus_tag="PWY43_000680"
     CDS             complement(2270..3649)
                     /gene="allB"
                     /locus_tag="PWY43_000680"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDL2135581.1"
                     /translation="MYDLIIKNGKIVLSDTVFNGNIAVNSGKIAAILEEGTEPDAKDV
                     IDVKGNYVFPGAIDTHTHLNDPGYEWREDYAHGTAAAVGGYTTIIDMPVQNEPALTNG
                     KIFDAKMAKVSPNAYVDYCFWGGLVPSNFEHLSELNDKGCVALKSFIGPVSPDYASLN
                     YGQTVEAMEIIQKFGGRAGFHCEDFSTIKWQEQRMKNEGRLDWRAFLDSRPFIAEMLV
                     TINIIEIAKATGCKAHICHVSHPDVAQKIKDAQEEGYDITAETCAHYLCFSEDDVIEK
                     GALFKCAPPLRSKSDVDRLWNYVVDGTFSGISSDHSPCSYDEKYNEILGQKINNVFDV
                     WGGISGIQSCVQVAFYEGCIKRGISPTVLANVMAKLPAKAFGIYGQKGDIQVGFDADF
                     TIIDPNKEWEIKAEDLQYVNKISAYVGKKGQGLPVCTIVRGKVIAQDGKLTDSKGFGK
                     FITKTKQGA"
     gene            complement(3749..4984)
                     /locus_tag="PWY43_000681"
     CDS             complement(3749..4984)
                     /locus_tag="PWY43_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDL2135582.1"
                     /translation="MERITQRITQNLETLKQFTATPGNGCTRLPFSPEARSAVNYLRD
                     LIVEVGLRVKEDEAGNIFGILEGEEPNLPCVMSGSHYDSVLHGGDYDGIAGVITALET
                     ARLLKESGKKFKRSFVVAGFNDEEGMRFGTGYFGSGAMLGLRDAEYCKKFLDKDGISI
                     YEAMRNYGLDPEKVQNAKWEEGSIGHFIETHIEQGPVLENQGLEIGLVTGIVGLHRHM
                     FTVHGRADHAGTTPMDMRLDAIDVATKVIAKIADWAREKNDGTVATVGYMNVLPGGIN
                     IVAEKVIFSVDIRSLNMANIEDITAKIDAALAEETKKIGSRFDKETKLVIQPVKLSEK
                     MLNILEEATKARQFSYKRLPSGAGHDSLEIGQKIPTVMIFVPSKEGRSHTPVEFTEYH
                     YFSKAAVLQQELAEQLLDE"
     gene            complement(5025..5240)
                     /locus_tag="PWY43_000682"
     CDS             complement(5025..5240)
                     /locus_tag="PWY43_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135583.1"
                     /translation="MTVKSTLESSFFKTKAGKMTLAFIVIMLAFALIMLGIYQVNNVF
                     IHIGFVLMLVAMLYSPVDVFILNRKKN"
     gene            complement(5250..6854)
                     /locus_tag="PWY43_000683"
     CDS             complement(5250..6854)
                     /locus_tag="PWY43_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL2135584.1"
                     /translation="MDIPVNFFMWFMALLPIITLVILMVKFQWGATEAAPVGLLITII
                     SGIFLYKADVTLIAIESAKGIWYALIILLIVWTAVLMYQVSDAAKAFLVIRSEMSKLF
                     PNELLLVLAMGWIFESFLQGITGFGVPVAVGAPLLIGIGVRPLWAVVLPLLGQAWGNT
                     FGTLAAAWDALAGSAGLEIGNEKYFLTALWSSAFLWFWIVITGLAIAWFYGRGKALKK
                     GLVATLIIATIQGGGELLLSQVNTTISAFIPACLSILAIVLISKMSMYKEVWRVEESP
                     IMDRTKTTETEEALPNMNLLQAFIPYIVLSVMTLAILVITPVNQFLSQFKFGFAFPET
                     VTGYGFLNKAYTSYSPIAIFTHASMFLFISSIIGLIYYQRKGWIAKGGTRAIFAKSIS
                     MTMPSGIAVVGLVIMSKIMGGTGQTAVLAEGMAAVLGKTYVILSPFIGLLGTFMTGSN
                     MSSNILFGDFQMTTSKLLNANSAAVLGAQTAGGAIGSAISPSKIILGTTTANILGKEG
                     DVLKTIIWITLLPILLIGVVAYFASL"
     gene            complement(6915..7847)
                     /gene="arcC"
                     /locus_tag="PWY43_000684"
     CDS             complement(6915..7847)
                     /gene="arcC"
                     /locus_tag="PWY43_000684"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL2135585.1"
                     /translation="MSKQTIVLALGGNALGNNLVEQREAVAKTAKVIVDILQQGKNIV
                     ITHGNGPQVGMIQRAMDDFAKSSGDEIPLPTSVAMSQGYIGIDLQNAIKYELVTRGIE
                     QKVSTILSQIEVDPKDAAFQNPDKPIGRFLTKEEADELEAKGIRTMEDAGRGYRIVVP
                     SPKPKRICELETIKTLVEAGHIVITCGGGGIPVVADENGRLVGVNAVIDKDNASSLLA
                     EKLNADYLVILTAVEKVAINWGKPNQEWLSELSTEQARQYIAEEQFAKGSMLPKVEAA
                     LKFAESGEGRRALITLLDKAAEGIAGETGTVIYS"
     gene            complement(7982..10981)
                     /gene="fdrA"
                     /locus_tag="PWY43_000685"
     CDS             complement(7982..10981)
                     /gene="fdrA"
                     /locus_tag="PWY43_000685"
                     /inference="COORDINATES: protein
                     motif:HMM:NF004760.0,HMM:NF018275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDL2135586.1"
                     /translation="MLKTVVKKGSYQDSVVLMLLTNAISGLEGVNKVSVMMATPANKD
                     IFAQSGLDTAELLEATANDMVVVADVDQEDVINVVMEKVEAFLKQKSQASSSHSGTEV
                     VKSWNKATASLPGANLAVISIPGAYAALEANRALDEGLNVFMFSDNVSVEDEKALKEK
                     AHRKGLVVMGPDCGTGIIQGVPIAFTNSVTPGSIGIIGASGTGIQELTTIIDRLGEGV
                     ENAIGTGGRDLYEDIGGITMLDAIEAMEQNDKVKVLIVISKPPAKAVREKISARLSRY
                     SKPVITLFLGEKPTFHEENFYHAYTLDEAARLAVALVRQEPIPTFAKNSTNSTACGKT
                     LKAYYSGGTLAGEAAMLLKDALRIEGSGSKADGFMFKQDGHIVVDLGDDIYTQGKPHP
                     MIDPAKRIESMRDAVDDATTGVILFDIVLGYGSHEDMATALIPTIQELQQKAKAQSRD
                     VAFVATVCGTRSDYQGYDETVRKLVEAGVEVCETNKAAVEKSLDLLGLPFDEPTKPIQ
                     PKTVVQGENSPASEQLLRLLSEKPKIINIGLKSFADVAEKFGCQVVQFNWQPPAGGNI
                     QLIKALNFLNEYEAVDIDEANRKVIAKVVAATPIIRDNVLAKTVIKELNDGKVILHAG
                     PPIQYKDMPNTVQGSCVGAVLFEKWATDEASARALLENGDITFMPCHHVNAVGPMGGI
                     TTANMPVWVVENATDGNFAYCTMNEGIGKVLRFGAYSDEVVKRLEWMRDVLGPTLGKA
                     IRSMENGLAVNPLVAKAIAMGDEFHQRNIAASMSFFKEVAPRITAMSDLAENDKYDVI
                     KFLADTDQFFLNIMMASCKAIMDGARTITEGTVVTAMCRNGVHFGIRIAGMGDEWFVG
                     PVNTPQGLYFTGYDGEDACPDIGDSAITETLGVGGLAMIAAPAVTRFVGAGGYEDALR
                     TSNDMMEICIDRNPNYIVPNWNFQGACLGVDARLVVEKGITPVINTGIAHKVAGFGQI
                     GAGTVRPPLACFEKAVLAYARKLGFNE"
     gene            complement(11063..12115)
                     /gene="allD"
                     /locus_tag="PWY43_000686"
     CDS             complement(11063..12115)
                     /gene="allD"
                     /locus_tag="PWY43_000686"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDL2135587.1"
                     /translation="MKLSREELKGLMKAKLMKAGLNDQHADITAEILTWSDERGYHSH
                     GAVRMEYYCERIFKGGITHNPKFEWKETGPASAIFEGDNGCGYVAATFAMEKAIEMAK
                     KSGIAIVGIRNISHSGAIGYYTEMAAKEGLLSISFCQSDPMAVPYGGSEPYYGTNPIS
                     FAAPADEGRNVVFDMATTVQAWGKILDKRSRGESIPPDWAVDVDGNPVTDPNKVNALV
                     PISGAKGYGLMMMVDIFSGILLGVPFGKHVSSMYHDLSEGRRLGQMHIVIDPACFCGA
                     EQFRENMAQSINELNEMSPAPGNDKVYYPGQRAEMRRDKYAATGGIEIVDKVYEYLIS
                     DDIHFDRYDHKNRFAD"
     gene            complement(12137..12922)
                     /locus_tag="PWY43_000687"
     CDS             complement(12137..12922)
                     /locus_tag="PWY43_000687"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDL2135588.1"
                     /translation="MGYLNGQTGYRQGLLETRSVIRKHNFVILETDGLVRNSIPNYHN
                     CDISILSSPALGASFVDYIAVVHPNGGCTQLGGNGIEVFVYLISGQLAVKNGDTDVVL
                     ESGGYIFSPADQVLSFINKSEHATKIYIHRRRYTALEGHQAITYVGNVNEIDYVEYEG
                     MKTCLIKDLLPSATDFGFDMNMHILLFKPGASHGYIETHFQEHGMLFLSGKGMYRLDD
                     EWIPVKKDDYVFMDSYCPQACYAVGDEDFVYIYSKECNRDVEL"
     gene            12996..13850
                     /locus_tag="PWY43_000688"
     CDS             12996..13850
                     /locus_tag="PWY43_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2135589.1"
                     /translation="MLKATQITLNTVQVQDTALVIEATHYLDDNIAPKANLIYLHGGG
                     LLYGTKEDLPQRHLETFTQAGLQIFAINYPLAPQAQIEEIISSIQQSIKHLQNQLHLE
                     LPYFLFGRSAGAYLALLTAVRLSQQIEIEKPKGVISYYGYGFLKDGWFDSPSPHYLKL
                     PLVIENTVQSLIATPSQEADLNTHFSCYVHARQTGKWKSLIYQGRDKFFYLDYSLRLA
                     ELNLPIFAAHATNDPDVPFAEFQALCEKFNPTKFVIAAELHDFDRDEQSKDTEELIQA
                     TIKFIQST"
     gene            complement(13955..15283)
                     /locus_tag="PWY43_000689"
     CDS             complement(13955..15283)
                     /locus_tag="PWY43_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL2135590.1"
                     /translation="MSENTNLQPIPANKRIMDLLSYLFLWLGGCVSIGTFALGSGQLE
                     KGLNLTQAFVAMLIGSLLLIICLCLNDQMSYKSGVPYAVQLKSAFGTKGGILPAMLRG
                     VPAIVWYGVQSWLGGSAINQISILLVGYDNIVLYFLLFQALQIWISTTGFHGLKWLEN
                     IGAVVIIASLAYMFYVCMTKFGGRIEENLINKEGTWGLPFVSAIVAFFGVNVLVMLNV
                     GDYVRELKAGYSTTKRGVVYFLAMVPTTVFMGIIGLMISSATGIANPINAFAQAADNK
                     FLVVITLVFILFAQMTTNLASNVVPPAYALMDAFKIKHKTSVVLIGLLAIATCPWLIT
                     SDASSKGVDIFVLTYTVFFGPIFAVLLVDYYILRKQKVDIAELYDENGNRRGVNWAAI
                     LAISVGAVIGLFNADISFLTATIPTGLVYYFGMKYMASSARFRKGTILEK"
     gene            complement(15379..16785)
                     /gene="allB"
                     /locus_tag="PWY43_000690"
     CDS             complement(15379..16785)
                     /gene="allB"
                     /locus_tag="PWY43_000690"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDL2135591.1"
                     /translation="MYDLIVKNGQLVTPDRIYPATIAVKEGKFAGFLAPDIEIQAKEV
                     VDAKGHLVYPGIIDCHAHLNEPGFDYREDFETGSRAAVAAGCTTLIDMPLNNDPSLIN
                     KDVFKLKHDNVSKHSFIDFALWGGIVGDYDDSPNSIKSNSADLVELEACGVAAFKGFT
                     CPNGDLFPTVNLGNVRKALEILKPFNALSGFHCEEYGQVKERVKEAKAKIGLTNQEKI
                     REFLDAHDVWVEFVATKNIIDMSRATGGRVHICHVTHPMVAQLVKDAIYEGLPVSGET
                     CPHYLGFTEDFVFEKGGPAKCTPPLRKKEDMEKLWDYVIDGTLSCVGSDHSPAADEEK
                     NNDTHDIWEAWGGLNGIQFFLPMMFDMVVHQRKLSPSLIAKVMGVNPAKLFGLYGRKG
                     AFEIGFDADIVIVDPEKPWKVEQEKLFTKGHVTCFNGLEGKGAATHTFIRGRLVAENG
                     MYKQDAIGYGEFIRPVKR"
     gene            16910..17773
                     /locus_tag="PWY43_000691"
     CDS             16910..17773
                     /locus_tag="PWY43_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDL2135592.1"
                     /translation="MTIKEQIINIPILKTTLYAQNAIEAAPIVMLHSRYNKTINLLIN
                     NQIFTLQPKESELSPISLITDFSITKFQQLSFQPNQPYRLNLHYSNDAIFSSKLTACT
                     SFSPNNVVWYANLAQKILQKSNAKGLNLLLQNSINDILLRAFKQYLVQAEDLYQLGEL
                     EKSTEYFTKLIGLGIGLTPSGDDFLCGFIATFQYFTQTQTAFYQTLTQKLRQHLDNTN
                     AISRRFLDCALQQQFSAPILSFFNHTQQRQEDFCLLAQRFENIGHSSGMDTLFGIYHA
                     CKWLLKKSIIT"
     gene            complement(17932..18369)
                     /locus_tag="PWY43_000692"
     CDS             complement(17932..18369)
                     /locus_tag="PWY43_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2135593.1"
                     /translation="MKSFIEDGGNRMKQIYALGLAFIVASVSLLSTKVQANQAYQTTK
                     QGLQYKELKAGSGKKPTLNDEVEVRFTSYNDKGEVFEGTFNQVPVILPIREMFSGLQQ
                     GLLLMPVGSVYEFNIPAHLGYQEEGSRAKKAALYRIELLRINP"
     gene            18506..21145
                     /gene="ppc"
                     /locus_tag="PWY43_000693"
     CDS             18506..21145
                     /gene="ppc"
                     /locus_tag="PWY43_000693"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDL2135594.1"
                     /translation="MNQQYSTIQHNIHMLGEFLGETIRDAQGNYILELIESIRVLSRN
                     SRAGDENAREQLLDKLANISNDDVLPVARAFSHFLNLTNIAEQHQTVSRHHIDESLGS
                     RSLDSLFLRLKSQNIPVEKVIKTVESLMIDLVLTAHPTEVTRRSLGHKHVEINKCLNL
                     LEHDDLTEAESYKIKRRLMQLIALAWHTNEIRTQRPTPVDEAKGGMAVIENSLWKAVP
                     EFCRQLNFYLEKHFGVQYPVNLAPVRFSSWMGGDRDGNPFVTAETTRRVLRMNRWKAT
                     ELFLQDIKNLAEELSITQCTPAFRAKYGNHLEPYRVLLKVLRQKLINTEIYYAELLEG
                     KISTIDESDILTSDQQLWEPLFDCYESLQACGMRIIANGALLDCLRRINCFGLGLSRL
                     DIRQESTRHEMAIAEITRYIGLGDYAHWTEDDKQAFLVRELSSRRPLIPTNWTPSPET
                     KEVLDTCKIVAEQPEGVISAYVISMAREASDVLSVHLLLKEAGCKFTIPVAPLFETLG
                     DLDHSEKVMTDLFNIGWYRGVINNYQMIMIGYSDSAKDAGMLAAGWAQYRAQEALVNL
                     CEKYNIELTLFHGRGGTIGRGGAPAHAALLSQPPRSLKNGLRVTEQGEMIRFKLGLPA
                     VAVESLELYASAILEANIFPPPEPKQQWRDVLAKASDISYQIYRDMVRGELDFVPYFR
                     SATPEQELSRLPLGSRPAKRNPKGGVDSLRAIPWIFAWMQNRLMLPAWLGAGQALQQL
                     IDEGNETLLKEMCNEWPFFSTRIGMLEMVFSKADIWLAEYYDQRLVEPKLHRLGNMLR
                     TQLQADLKTVLALAHDGQLMADLPWIAESIALRNIYTDPLNLLQVELLDRIRKQGENA
                     DPVLEQALMITITGIAAGMRNTG"
     gene            complement(21287..23620)
                     /gene="lptD"
                     /locus_tag="PWY43_000694"
     CDS             complement(21287..23620)
                     /gene="lptD"
                     /locus_tag="PWY43_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDL2135595.1"
                     /translation="MKQRYSLLSLAVMAACYSNSAAADLKQQCLLGVPHFQGEVVEGD
                     QLRMPVTIESDSAVINQPRDATYTGDVTIKQGNRSIWAEQVRVEQDGENARMAYLQGN
                     FNYQDNLIQATGADATINLLSKDANLTNANYQLVGRQGRGTAESGEFGNSKRTLKNAS
                     FTSCLPNDNSWQIEANEMIQHVDEEYAEMWHARFKVLGMPVFYSPYLQFPIGDRRRSG
                     LLIPSFERSSKNGWTYSQPFYWNIAPNMDSTITPTYYSRRGWQISPEFRYLTKIGQGL
                     VATEYMAKDRLSEYQPGNDDRKRYLFHWRHAMSFLTDWRFSVDYTKVSDRRYFSDFDS
                     DYGSSTDGYATQNFKLGYYQPNYNISISGKKFQTFDELDIGPYRVLPQIDFNYYKNDL
                     IKGGDFKFFAQAARFENDSKLMPKAWRFHAEPTLNFPLANRYGSLNLETKLYATYYQQ
                     TKGSESTEEIKKSVSRVLPQIKLDFKTVLESDKQLFSGYTQTLEPRVQYIYRPYKDQS
                     DIGSKAHRSVSLGYDSALLQNDYFSLFNDRRYSGLDRISSANLVTAGGTTRFFKEATG
                     EEVFNFSLGQTYYISPSKIDDTSDNSTKGHTTSWAAESNWKFHKNWNWHGSYQYDTRL
                     NQTALANMSLQYKPQSDKLIQLNYRYASKDYINQNLNTNKYGQSIKQVGGVLGWELTD
                     KVSVMTSYYHDLALKKPVEGQISLNYNTCCWAANVYVARRLVSTPVGSSDTINDFYYD
                     NKFGINFELRFGTNYSNGVTRMLKRGIIPYTEPYGIN"
     gene            complement(23694..25061)
                     /gene="hemN"
                     /locus_tag="PWY43_000695"
     CDS             complement(23694..25061)
                     /gene="hemN"
                     /locus_tag="PWY43_000695"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL2135596.1"
                     /translation="MSEIIWDLALIQKYNHSGPRYTSYPTALEFNENYTNEDFIRAAA
                     RYPERPLSLYVHIPFCHKLCYFCGCNKVITRHRHKVEIYLDYLEREIKTRAPLFQNRL
                     VTQIHWGGGTPTYLDEDQSARLMQMLRANLDVSDDAEISIEMDPREIELSMLDHLRAI
                     GFNRLSMGIQDFDKEVQRLVNREQDEEFIFALMKRAKELGFVSTNVDLIYGLPKQNVE
                     SFMYTLQRVVELNPDRMSVFNYAHLPSRFAAQVKIKEELLPAPETKLTILQNSIEFLG
                     KNGYKFIGMDHFAKPDDELAIAQEKGILHRNFQGYTTQEECDLLGMGVSAISLLGDTY
                     AQNRKELQQYYADVETNGIALHKGIALSQDDCIRRDVIKALICNFKLDFATIEKQYGI
                     DFKRYFAEDLAFLAPLAEDELLTMNDDSIVVSPRGRLLIRNICLCFDVYSRQLARRQQ
                     FSRII"
BASE COUNT         7605 a         5606 c         4440 g         7481 t
ORIGIN      
        1 gttaggttta aaaatacttc ataccaatat cccctaagtt gggtaaaacc ttatgaaata
       61 ggatacaagc ggttagtttc tctcaatttt ttaccaaagg atcacgcaat gagccaaccg
      121 gctttacggc aaatatcccc ctcgcagcta gaagatattc gtctgcaaat ggtgaagttt
      181 gcctttttac agttaaaaaa taacgaactt gccgaagatg tggtgcaaga ggcgtttgtc
      241 agcgcttata aatatgccga cagcttcaaa ggtgaaagtg cgttaaaaac gtggattttt
      301 gcaattttaa aaaacaagat cgtagattta gtgaaagcca aaggcaaatt ggtggcagta
      361 tctgagctga tggaagaaga cgatcacgat cttgccgata aattgtttaa cgaaaatggc
      421 ggttgggata aagatgctta tcaatctaat gagtggaaga acaccgaaag ccatacctat
      481 tccgctcaat tctggcaagt atttgaattt tgcctaaata atctgccggc aaatcaagca
      541 agggtgttta tgatgcgttc ccatttggaa atggaaaccg aagaaatttg cgcagaatgt
      601 gagatttcac cagcaaatgt gcatacctta ctgtatcggg caagattgca gttacaggtc
      661 tgtttaagtc aaaaatggtt tggaggttaa atgttaaaat gtaagcaaat taccgaatta
      721 gtttctctgt cccaagaaca aaaactgaca ttaaaacaac gtgttaatgt gcaagttcat
      781 ttaatgcttt gttctcgttg cagagccttt agccaaaact gcaaaaccgt agataagcta
      841 acgaaagaat ttaactctta gcgtaaaagc aagcggtaaa atttttaata aattttaccg
      901 cttgtcatct tattgatgtt ctagcactgt ccctttacgg aaagatgctg aacttttcaa
      961 tactttcata agaaagtagt agcttaaacc agaaggaagt aaactcacat accaagaaag
     1021 atcaaccaca atcaatgagc aaagtgaacc aattgtaata gctatgattg cagcccagtt
     1081 aatccctttg aaaatacctt ctttattata taagtcattt acgtttaaag tacgttttct
     1141 taagatgtag taatcgacga ccattaccgc aaaaataggg cctaagaatg ccgagtaaat
     1201 tttaataaac aaagataatc catctgctga ttcagcagtt accaacttcc aaggcattac
     1261 acatatagaa agaatgccca ctaaaatggt ggcatgcgac catttcattt taaatgaatc
     1321 cattaaaatg taagatggcg gaacaatatt attcaacacg tttgtcgtaa cctgtgcaaa
     1381 agcaatgaat aataacgtta ttactgttaa aaatttatta tccattgcag ttgcaaatac
     1441 ttcaattggg tcactattgc ctgtagctgc cgtaactaac aaccctatta aacccataaa
     1501 taaggttaca gggaggattg ataatacata aatcacacct gttgctgttg gggttgttcc
     1561 ttttttaacg tggcgagaat aatcggaagc atttaaaatc attgttgaat aaatacccaa
     1621 gaatgctgta gttacagccc agaatggcat tccccaagtg ccttcaatat tggcaaagcg
     1681 ctcgcctatt tctacagaga attttgtatt cacaacataa agcatataag caagaatacc
     1741 gatgaggaac acacaagaaa cattttccaa ccatttaata cctttaaaac cattaatgga
     1801 taagccaact tgaagaacag tgaatagcac ataaacaaca ggcaagttat caaaaccgaa
     1861 caacagtttt aaacacatat tgatagcacc tgcccccacc caactttgga atccaaacca
     1921 aacgattgca gggacaccac gtaaaatacc aggaattttt acacctgttg taccaaaaga
     1981 gcttcgtagt tggattggga atggaatacc atatttatgc ccgccattgc caattagcac
     2041 aaggccgata gcaatcacaa tacaaccaaa cgacattgca ataatcgctt gtgttatcgt
     2101 taacgctcca atcaggcttg cccccataga aaatgtacca atagaaacac agccaccaaa
     2161 aaacttgccc aataggaaaa gttatccata atgcgttctt tggttggctg taagtcaaca
     2221 tttacttctt ttgctaactc agggttaagt tgtgtgttgt gttgcattgt tatgctcctt
     2281 gttttgtttt agtaataaat tttccaaaac ctttgctgtc ggtgagtttc ccatcttgag
     2341 caatcacttt accacgtacg atggtgcaaa ctggtaaacc ttgacctttt ttgcccacat
     2401 aagcggagat tttattcaca tattgcagat cttccgcttt gatttcccac tccttgtttg
     2461 ggtcaataat cgtaaaatct gcatcaaatc caacttgaat atcgcctttc tgaccataaa
     2521 taccgaacgc tttagctggt aattttgcca ttacatttgc caatacagtt ggagatattc
     2581 cacgctttat acaaccctca tagaaagcca cttgtacaca actttgaatg ccactaatcc
     2641 cgccccatac atcaaataca ttgttaattt tttgacctaa aatttcattg tacttttcat
     2701 cataagaaca aggagaatgg tcgctactaa tgccgctaaa tgtcccatct accacataat
     2761 tccatagacg atcaacatca gattttgagc gaagtggcgg agcacatttg aacaacgccc
     2821 ctttttcaat cacatcatct tcactaaagc agagataatg tgcacaggtt tcagcggtaa
     2881 tatcatagcc ctcttcttga gcgtctttaa ttttttgagc aacgtctggg tgactaacgt
     2941 ggcaaatatg cgccttacag cccgtcgctt tagcaatttc aataatatta atggtgacaa
     3001 gcatttctgc aataaacgga cgggaatcta aaaatgctcg ccaatctaaa cgcccttcgt
     3061 ttttcattct ttgctcttgc catttgatgg tggaaaaatc ttcgcaatga aaacctgcac
     3121 gaccaccaaa tttttggata atttccattg cctcaacagt ttgaccatag ttaagtgaag
     3181 cataatcagg agaaacaggc ccaataaagg actttaacgc gacacagcct ttatcattta
     3241 attcacttaa atgctcaaaa ttacttggta ctaaaccacc ccagaaacag taatcgacat
     3301 acgcatttgg cgatacttta gccattttgg catcaaatat tttgccatta gttaaagcag
     3361 gctcattttg cacaggcata tcaataatgg tggtatatcc gccaactgcg gcggctgtac
     3421 cgtgtgcata atcttcacgc cattcataac ctgggtcatt aagatgggtg tgagtatcta
     3481 ttgcccctgg aaaaacataa tttcctttaa catcaataac gtcttttgca tctggctctg
     3541 tgccttcttc taaaatagca gcaatcttac ctgaattaac ggcaatatta ccgttgaaaa
     3601 cggtatcgga taagactatc ttcccatttt ttatgatgag atcatacata gtcaaatact
     3661 ccttatagtt gtgtttgtat taaaatttat aggcaattta caagcggtca aatttaaaaa
     3721 aaactttgca aatcccctca tttgaatatt attcgtctaa taattgctca gctaattctt
     3781 gttgtaatac cgctgctttt gagaaatagt gatactcagt aaactctact ggagtatggc
     3841 tacgaccctc tttacttgga acaaaaatca tcacagttgg aattttttgc ccaatttcta
     3901 atgaatcgtg cccagctcca cttggcaagc gtttatagct gaattgacga gctttagtag
     3961 cctcttctaa aatgtttaac attttttcag atagcttaac gggttgaata accaattttg
     4021 tctctttatc aaatctcgaa ccaatttttt tagtttcctc cgctaatgct gcatcaattt
     4081 ttgccgtaat atcttcaata tttgccatat ttaaagaacg aatatcaaca ctaaagatca
     4141 ctttttctgc cacaatgtta atcccaccag gtagaacatt catatagcca accgtcgcta
     4201 cagtgccatc atttttctct cttgcccagt ctgcaatttt agcaatcact ttagttgcca
     4261 catcaatggc atctaaacgc atatccattg gggttgtgcc tgcatgatct gctcgcccat
     4321 gaaccgtaaa catatgacga tgtaagccaa caatacccgt tactaaacca atttcaagcc
     4381 cttgattttc taacaccggt ccttgctcaa tatgggtttc aataaagtgg ccgattgaac
     4441 cctcttccca ttttgcattt tgaacttttt ctggatctaa accataatta cgcattgctt
     4501 cataaatgga aatgccatct ttatctaaga attttttgca atactctgca tcacgaagcc
     4561 ctaacattgc acccgatcca aaataacctg tgccaaaacg cataccttct tcgtcattaa
     4621 agcctgcaac gacaaagcta cgtttaaatt tttttccgct ttctttcaat aaacgagccg
     4681 tttccagtgc cgtaatcacg cctgcgatac catcataatc gccaccatga agcacactgt
     4741 catagtgaga accagacatc acacagggta aattgggctc ttcgccttct aaaataccga
     4801 aaatatttcc agcttcatct tctttcacac gaagaccaac ttccacgatt aaatctctta
     4861 aataatttac cgcacttctc gcctctggtg agaatggtaa acgtgtacaa ccattacctg
     4921 gtgtcgcagt aaattgtttt aaagtttcta aattttgcgt aatacgctga gtaattcgtt
     4981 ccatttttta ttccttttta taaacgaaaa tatttcgcca tacgttaatt tttcttacga
     5041 tttagaataa atacatcaac tggcgaatat aacattgcca ccaacattag cacaaatcct
     5101 atatgaataa agacattatt cacttgataa atgccaagca taatcaatgc aaacgctaac
     5161 ataatcacaa tgaaagcaag agtcattttg cctgctttag ttttaaaaaa actactttct
     5221 aatgtagatt ttactgtcat aaactcctcc taaagtgaag caaaataagc cacaacgcca
     5281 attaataaaa taggaagtaa cgttatccaa ataattgttt ttaacacatc gccttcttta
     5341 cctaaaatat tagccgttgt cgtacctaaa attatcttac ttgggctaat tgcactgcca
     5401 atagcgccgc ctgcagtttg ggcaccaagc acagcagctg aattcgcatt caggagttta
     5461 gaggtcgtca tttgaaagtc accaaataaa atattagaac tcatattact acccgtcata
     5521 aaagtaccta acaaaccaat aaatggtgat aaaatcacat acgttttacc taataccgca
     5581 gccattcctt cagccaacac ggcggtttgc cctgtccccc ccataatttt cgacataatc
     5641 actaagccta ctaccgcaat gcctgatggc attgtcatcg agatagattt tgcaaaaatt
     5701 gctcttgttc ctcctttggc tatccagccc ttacgttggt aataaatcaa gccaataatt
     5761 gaagagataa ataagaacat acttgcgtga gtaaaaatgg caataggcga ataagaggta
     5821 taagccttat tcaaaaagcc atagcctgtt actgtttcag ggaatgcaaa gccaaattta
     5881 aattgtgata agaactgatt cactggcgta ataacaagaa ttgccaatgt cattactgat
     5941 agcacaatat acggaataaa ggcttgcagt aaattcatat ttggcaaggc ttcttccgtt
     6001 tcggtggttt tagttctatc cataattgga ctctcttcca cacgccaaac ctctttatac
     6061 atactcattt tgctgataag cacaatcgct aaaattgaca aacaagcagg aataaacgct
     6121 gaaattgttg tatttacctg gctaagtaac aattcgccac caccttgaat agtggcaata
     6181 attaaggtgg ctactaagcc ttttttcagc gctttgcctc taccataaaa ccacgcaatc
     6241 gctaagcctg taatgacaat ccaaaaccat aagaatgcag aagaccaaag tgctgttaaa
     6301 aagtattttt cattaccaat ttcaagccca gctgagcctg ctaaagcatc ccaagcagcc
     6361 gctaatgtac cgaaagtatt accccaagcc tgaccgagca atggcaatac taccgcccat
     6421 aatggacgaa caccaatccc aattaatagt ggagcaccca ccgcaacagg cacaccaaaa
     6481 cctgtgatac cttgtaagaa actttcaaaa atccagccca ttgccaatac cagtaataat
     6541 tcgtttggga ataatttact catttccgaa cgaatgacta aaaaggcttt tgcagcgtca
     6601 ctgacttgat acattaatac cgcagtccaa acaattaaca aaataattaa tgcataccaa
     6661 atcccttttg ctgattcaat tgcaattaat gtaacatctg ctttgtataa aaaaatgcct
     6721 gaaataatgg taattaataa tccaacagga gcagcttctg ttgctcccca ttggaattta
     6781 accattaaaa taaccaatgt gataatcggc aataacgcca taaaccacat aaaaaaattc
     6841 actggaatat ccattttgtt taaaacaacc taattacaaa aaattgaaaa aaatgaccgc
     6901 ttgtgctaga tattttacga ataaattacc gtacctgttt cgcctgcaat accttctgcc
     6961 gctttatcta acaatgtgat taatgctcta cgcccttcgc cactttctgc gaatttcaat
     7021 gcagcttcca ccttcggcag cattgagcct ttcgcaaatt gctcttcggc aatatattgg
     7081 cgagcctgtt cggtgcttaa ttcagaaagc cactcttggt taggcttacc ccagttaatg
     7141 gcaacctttt ctactgctgt taagataact aaatagtcgg cattaagttt ttcagccaat
     7201 agcgagctgg cgttgtcttt atcaataaca gcatttacac ccactaatcg accattttca
     7261 tcagcaacca caggaatacc tccaccgcca caagtaatta caatatgccc tgcttccact
     7321 aatgttttaa tagtttccag ctcacagata cgttttggtt ttggcgaagg caccacaata
     7381 cgatagccac gcccagcatc ttccattgtg cgaatacctt ttgcttctag ctcgtcagct
     7441 tcttctttag ttaaaaatct gcctattggt ttgtctggat tttggaatgc agcatctttt
     7501 ggatcaacct caatttgcga caaaattgta ctgactttct gctcaatacc acgagtaacc
     7561 aattcatact taatcgcatt ttgtaaatca atgccaatgt aaccttgact cattgcaaca
     7621 ctggtgggta atggaatttc atcgcctgaa ctcttggcga aatcatccat tgctcgctgg
     7681 atcatcccca cttgcggtcc attaccgtgg gtaatcacaa tatttttacc ctgttgtaag
     7741 atatccacaa tcactttcgc tgtttttgca acggcttcac gctgctctac taaattattg
     7801 cctaatgcat taccgcctaa ggctaatacg atggtttgtt ttgacattat ttctcctttt
     7861 tatccctact accccatacg agccgtatgg ggttctatta tttcaaccac gccgtggctc
     7921 ataaagccct aggacttggt ttattcaacc tagcacgcct tggctaggtt aatgcaggat
     7981 tttactcgtt aaaccctaac tttctcgcat acgcaagcac agctttttca aaacaagcta
     8041 gtggtgggcg tactgtgcct gcaccaattt gaccgaaacc agccactttg tgggcaatac
     8101 ctgtgttgat tacaggggta atgccttttt caaccactaa tcttgcatca acacctaagc
     8161 aagccccttg gaagttccag ttcggcacaa tgtagttagg gttacggtcg atacaaattt
     8221 ccatcatatc gttactggtg cgaagagcat cttcataacc gccagccccc acaaaacgag
     8281 ttaccgctgg tgcggcgatc atagccaaac cacccacacc taaggtttca gtaatggctg
     8341 agtcgccaat gtcagggcaa gcatcttcgc cgtcatagcc agtaaaatac aagccttgtg
     8401 gtgtattcac agggccaaca aaccattcat cgcccatacc cgcaatacga ataccaaaat
     8461 gcaccccatt gcgacacatt gcagtcacaa ccgtaccctc tgtaatggtt ctcgccccgt
     8521 ccataatcgc tttacagctt gccatcataa tgtttaagaa gaattgatcg gtatccgcta
     8581 aaaatttaat cacatcgtat ttatcatttt ctgccaaatc actcattgca gtaatgcgag
     8641 gagcaacttc tttgaagaaa gacattgagg ctgcaatatt tctttggtgg aactcatccc
     8701 ccatcgcaat cgctttcgcc accagtggat tgactgccaa accgttttcc attgaacgga
     8761 ttgccttgcc aagtgttgga cctaatacat cacgcatcca ttctaaacgt ttcaccactt
     8821 catctgaata cgcaccaaaa cgcaatacct taccgatacc ttcattcatt gtacagtaag
     8881 cgaaattgcc atctgtagca ttttctacta cccaaacagg catatttgct gtcgtgattc
     8941 cgcccatcgg acccaccgca ttcacatggt ggcacggcat aaatgtaatg tcgccgtttt
     9001 ctaataaagc acgagccgag gcttcatctg tcgcccattt ttcaaataat actgccccaa
     9061 cgcaagaacc ttgcaccgtg ttcggcatat ctttgtattg aattggtgga ccagcgtgta
     9121 agatcacttt gccatcgttt agctctttga tcaccgtttt tgccaacaca ttatcacgaa
     9181 taattggtgt ggctgccaca accttagcaa tcactttacg atttgcctca tcaatatcca
     9241 ccgcttcata ttcgtttaag aaattcagtg ctttgatcaa ctgaatgtta ccacctgctg
     9301 gaggttgcca gttaaattgc actacttggc agccaaattt ttctgctaca tctgcaaaac
     9361 ttttcaaacc aatattaatg attttaggtt tttcagataa taagcgtaat aactgctcag
     9421 aagctgggct attttcgcct tgcactacgg ttttcggctg gattggtttg gttggctcat
     9481 caaatggtaa acctaataaa tctaatgatt tttctactgc agctttattg gtttcgcata
     9541 cttcaacgcc tgcttctacc aatttacgca cggtttcatc atagccttgg taatcgctgc
     9601 gagtaccgca tacggttgcc acaaaagcaa catcacggct ttgagcttta gctttttgtt
     9661 gcagttcttg aatggtcgga attaaggctg ttgccatatc ttcgtgcgag ccgtatccta
     9721 acacaatgtc gaataaaatc acgccagtag tcgcatcatc aaccgcatca cgcatagatt
     9781 caatacgctt ggcggggtca atcatcgggt gcggtttgcc ttgagtataa atatcatcgc
     9841 ctaaatccac cacgatgtgt ccatcttgtt tgaacataaa cccatcggct ttcgagccgc
     9901 tgccttcaat gcgtaacgca tctttcagca gcattgccgc ctcgccagcc aacgtaccgc
     9961 ctgaatagta ggcttttaag gttttaccgc aagcggtcga atttgtcgaa ttttttgcaa
    10021 atgttggaat tggctcttga cgcactaatg ccaccgctaa acgagccgct tcgtctaggg
    10081 tgtaagcgtg atagaagttt tcttcatgga atgttggttt ttcgcccaag aataaggtaa
    10141 taacaggctt gctataacgg cttaagcgag cagagatttt ctcacgtacc gcttttgctg
    10201 gtggtttaga gatcacgatc agcactttta ctttgtcgtt ttgttccatc gcttcaatag
    10261 catctaacat tgtgatacca ccgatgtctt cgtataaatc acgccccccc gtaccaatcg
    10321 cattttccac gccttcgcct aagcggtcga taatcgtggt taattcttgg atacccgtac
    10381 cagacgcacc aataataccg attgaacctg gcgttacgct gttagtaaat gcaatcggca
    10441 caccttggat aatccctgtt ccacaatctg gccccattac cactaaacct ttacgatgtg
    10501 ctttttcttt taacgctttc tcatcttcca cacttacgtt atcgctgaac ataaaaacgt
    10561 ttaatccctc atccaacgca cggttcgctt ccaatgcagc ataagcacct ggaattgaaa
    10621 tcaccgccaa gtttgcacct ggcaaagaag cggtcgcttt attccaagat tttaccactt
    10681 ccgtaccgct gtgagacgag cttgcttgag atttctgttt taaaaatgct tctacttttt
    10741 ccattaccac attgatcaca tcttcttgat caacatctgc gactaccacc atatcgttgg
    10801 cggtcgcttc taataattcc gcggtgtcta agccactttg agcaaaaata tctttgtttg
    10861 ctggggttgc catcattaca gatactttat ttaccccttc taagcctgaa atggcattag
    10921 tgagcaacat taacactacc gagtcttggt agctgccttt ttttactact gtttttaaca
    10981 tattattctt cctaatttta taagcctata caagctgtat agggttcttt gaattcagcc
    11041 acactatggc tatatgataa gattagtccg caaaacggtt tttatgatcg tagcggtcaa
    11101 aatgaatatc gtcactgatt aagtattcat agactttatc cacaatttca ataccacctg
    11161 ttgcagcgta tttatcacga cgcatttcag cacgttgccc tgggtaatac accttatcgt
    11221 tacctggtgc tggagacatt tcatttagtt cattaattga ttgtgccata ttttcacgga
    11281 attgttctgc accacagaaa caagcaggat caatcacaat atgcatttga cctaaacgac
    11341 gaccttccga taaatcgtgg tacattgaag aaacgtgttt gccaaatggt acccctaata
    11401 aaatacctga gaaaatatct accatcatca ttaaaccata acctttcgct ccagaaattg
    11461 gtactagagc attcactttg ttcggatcgg ttacaggatt gccatcaaca tcaactgccc
    11521 aatctggtgg aatagattcg ccacgagaac gcttatctaa aattttaccc caagcttgaa
    11581 cggtcgttgc catatcaaac acgacatttc tgccttcatc tgcaggtgct gcaaaagaaa
    11641 ttgggtttgt gccgtaataa ggttcagaac cgccataagg caccgccata ggatctgatt
    11701 gacaaaatga aatagataac aagccttctt tcgctgccat ttctgtgtaa tatcctatcg
    11761 caccactatg agaaatgtta cggataccta caattgcaat gcccgatttt ttcgccattt
    11821 caatcgcttt ttccatcgca aaggttgcag cgacataacc acagccgtta tcgccttcaa
    11881 aaatcgctga ggcaggccct gtttctttcc attcaaattt aggattatgt gtgatgccac
    11941 ctttaaaaat acgctcgcaa taatactcca tacgaacggc accgtgagag tggtagccac
    12001 gttcatcaga ccacgtcaga atttctgctg taatatctgc gtgctgatcg tttaaacccg
    12061 ctttcattaa tttagctttc attaagcctt ttaattcttc tcttgatagt ttcattgtca
    12121 attcctcaac gttaaattaa agttctacat cacggttaca ctctttcgag tagatataga
    12181 caaaatcctc atcacctacc gcataacagg cttgtgggca gtaggaatcc ataaaaacat
    12241 aatcatcttt tttcacagga atccattcat catctaaacg gtacattcct ttacctgaaa
    12301 gaaacagcat tccgtgttct tggaagtgcg tttcaatata accatgcgac gcaccaggct
    12361 taaataacaa aatgtgcata ttcatatcaa agccaaaatc agttgcagaa ggaagtaaat
    12421 ctttaattaa acaggttttc attccttcat attcaacata gtcgatttca ttcacattac
    12481 ctacataagt gattgcctgg tgaccttcta acgcggtata acggcgacgg tgaatataga
    12541 ttttagtagc gtgttccgat ttattgataa aactcagcac ttgatcggca ggcgaaaaga
    12601 tataaccacc gctttctaga accacatcag tatcaccatt tttaacagcg agctgcccgc
    12661 taatcaaata aacaaaaacc tcaataccgt taccacctaa ttgtgtgcag ccaccattcg
    12721 ggtgaactac agcaatataa tcaacaaaag atgcaccgag tgcaggagag gataaaatac
    12781 taatatcgca gttatggtaa tttggaatac tatttctcac taaaccatct gtttccaaaa
    12841 tcacaaaatt atgcttacga attaccgaac gtgtttcaag taatccttgt cgataacctg
    12901 tttgaccatt caaatatccc ataatattct ccttcttttt atataagtta agttgaatta
    12961 ttttgtacta taatatacaa aaatagaggt tcattatgct aaaagcaaca caaatcacat
    13021 taaatacggt tcaagtccaa gacacagctc ttgtaattga agcaacccat tatctagatg
    13081 ataatatcgc acctaaagcg aatcttattt atctacacgg cggcggccta ctttatggta
    13141 caaaagagga tttgcctcaa cgacatcttg aaacatttac tcaagctggt ttgcaaattt
    13201 ttgcaataaa ttacccgctt gcaccacagg ctcagattga ggaaattata agctccattc
    13261 aacaatctat aaaacattta cagaaccagc tacacctaga actcccttat tttttattcg
    13321 gacgttctgc tggagcttat cttgcattac tgactgctgt cagacttagc cagcaaatag
    13381 aaattgaaaa gccaaaaggt gttatctctt attatggtta tggctttttg aaagatggtt
    13441 ggtttgatag cccaagccca cactatttaa aattaccatt agtcattgaa aataccgttc
    13501 aatcactaat agccactcca agccaagaag ctgatttaaa cacacatttc agttgttatg
    13561 ttcacgctcg ccaaactggc aagtggaaat cattaattta tcagggcaga gataaatttt
    13621 tctacctcga ttacagctta cgtttggcgg aattaaatct accgattttc gctgctcacg
    13681 ccacaaacga ccctgatgtg ccgtttgcag agtttcaagc cctgtgcgaa aaattcaacc
    13741 caaccaaatt tgtcatcgcc gccgaattac acgatttcga ccgagatgaa caatcgaaag
    13801 atacagagga attgattcaa gcgacgataa aatttattca aagcacctag cacgagaagt
    13861 gctaggttac ataataagtc cctctgggat tttacaaagt cacagcgtgt gctcagctta
    13921 ttgaacccca tacggctcgt atggggttat gagcttattt ctccaaaatc gtgccttttc
    13981 tgaaacgtgc acttgatgcc atatatttca taccaaagta ataaaccaat cctgttggga
    14041 ttgtcgctgt taggaatgaa atatcagcat taaataaacc gataaccgca ccaacagaga
    14101 tagctaaaat cgcagcccag tttacccctc tgcgattgcc gttttcatca taaagttcag
    14161 caatatctac tttctgtttg cgtaaaatat agtaatccac caataacacc gcaaagatcg
    14221 gaccgaagaa taccgtgtaa gttaatacga aaatatctac gccttttgat gaagcatctg
    14281 aggtaattaa ccaagggcaa gtcgcaatcg ctaataagcc gataagcact accgaagttt
    14341 tgtgcttgat tttgaatgca tccattaacg catatgcagg tggtacaacg ttacttgcaa
    14401 ggttggtcgt catttgagcg aataagataa atactaatgt aataaccact aagaatttat
    14461 tatcggctgc ttgtgcgaac gcattgattg ggtttgcgat acctgttgct gaggagatca
    14521 ttaaaccgat aatccccata aataccgttg ttggtaccat cgctaagaaa taaacgacac
    14581 cacgtttagt agttgaataa ccggctttta actcacgcac atagtcacca acattaagca
    14641 ttactaatac gtttacgcca aagaatgcta caatcgcact cacaaatggt aagccccatg
    14701 taccttcttt attaataagg ttttcttcaa tacgcccacc gaatttggtc atacaaacgt
    14761 aaaacatata agcaagagag gcaataatca ctaccgcacc aatgttttcc aaccatttca
    14821 agccgtggaa gccagtggta gaaatccaaa tttgcagtgc ttggaatagc aagaagtaaa
    14881 gcacgatatt gtcataacca actaataaaa tagaaatttg gttgattgca gaaccgccta
    14941 accaactttg cacaccatac caaacaatag ccggcacacc acgcagcata gcaggtaaaa
    15001 taccgccttt tgtcccaaat gcacttttta attgaacggc ataaggcaca ccacttttat
    15061 agctcatttg gtcgtttaaa cataaacaaa taatcaacaa caatgaacca attagcattg
    15121 caacaaaagc ttgggttaag ttcagccctt tctctaattg tcctgaaccg agggcaaagg
    15181 tgccaataga tacacagccc cctagccaaa gaaagagata agaaagtaaa tccataatcc
    15241 gcttatttgc cggaattggt tgtaagttgg tattttcaga cattgtgtct ctcctgtaat
    15301 aacattgttt tatttttatg ataataaaag agcggtcatt ttttcagata tttttgaaaa
    15361 attgaccgca cttaaattct agcgtttgac tggacgaata aactctccat agcctatcgc
    15421 atcttgtttg tacataccgt tttccgcaac taaacgacca cgaatgaaag tatgcgttgc
    15481 cgcacctttg ccctctaagc cgttgaagca ggtaacgtgc cccttggtaa agagtttttc
    15541 ttgttctact ttccacggtt tttctggatc aacaatcaca atatccgcat caaaaccgat
    15601 ttcaaaagcc cctttacgac catataaacc aaatagtttt gctggattca cacccattac
    15661 tttggcaatt aaacttggac tgagtttacg ttgatgcacg accatatcaa acatcatcgg
    15721 caggaaaaac tgaataccat ttaaaccacc ccaagcctcc caaatatcgt gagtgtcatt
    15781 atttttttct tcatcggctg ctggagagtg atcgctaccc acgcaagaaa gcgtaccatc
    15841 aatcacataa tcccacagtt tttccatatc ctctttttta cgaagcggcg gcgtacattt
    15901 cgcagggcca cctttttcaa acacaaaatc ttcggtaaaa cctaaataat gtgggcaagt
    15961 ttcaccagac actggcaaac cttcgtaaat cgcatcttta accaactgag ccaccattgg
    16021 gtgtgtaacg tggcaaatat gcactcgtcc acctgtggca cgggacatat caatgatatt
    16081 ttttgttgcc acaaactcta cccacacatc gtgagcatct aaaaattcac ggattttttc
    16141 ctgattggtt aagcctattt tcgcttttgc ctctttcaca cgctctttta cttgaccata
    16201 ttcttcacag tgaaagcccg ataaggcatt aaacggcttt aagatctcta acgctttgcg
    16261 tacgttccct aaattaacag ttggaaataa atccccattt gggcaggtaa atcctttaaa
    16321 agcagctacg ccgcaagctt caagctcaac aagatcagcg gaattacttt taatggagtt
    16381 cgggctatca tcataatccc ccacaattcc gccccaaagg gcaaaatcaa taaaggaatg
    16441 cttactcacg ttgtcgtgtt taagtttaaa aacgtctttg ttaattaaag aaggatcatt
    16501 attaagtggc atatcaatta aggttgtaca acctgctgct acagctgcac gagagcctgt
    16561 ttcaaaatct tcacgataat caaaacccgg ttcatttaag tgagcgtggc aatcgataat
    16621 acccggataa accaaatgcc ccttggcatc aaccacttct ttcgcttgga tttcaatgtc
    16681 cggcgctaaa aaaccggcaa atttaccctc ttttaccgca atagtggcag gataaattct
    16741 atcaggtgta actaattgac cgtttttaac gattaaatca tacataataa accttctttt
    16801 tattgacttt taatggtttg aaaggaaaat aaaaaatgtg gaatcgtatt tcaactttgc
    16861 aagtatacta atcaataaag ttactacaaa tcaataggta ttttacagaa tgaccataaa
    16921 agagcaaata ataaacatcc ctattttaaa aaccactctg tacgctcaaa atgcaataga
    16981 agctgcacct attgtaatgc ttcactctcg ttacaataaa actattaatt tattgattaa
    17041 taatcagatt tttacattac agccgaaaga aagtgagctt tcaccgatca gtctaatcac
    17101 agatttctca ataacaaaat ttcaacaact ctcattccaa cctaaccaac cttatcggct
    17161 caatttacat tattcaaatg atgcaatttt ttcttcaaaa ttaaccgctt gcacttcttt
    17221 ttcaccaaac aatgtggttt ggtatgcaaa tcttgcgcaa aaaatcttgc aaaagtcgaa
    17281 tgccaaagga ctgaatttat tattacaaaa cagtattaac gatatactac tccgtgcatt
    17341 caagcaatat ttagttcaag ctgaggatct ttatcaacta ggcgaattag aaaaatcaac
    17401 ggaatatttc accaaactta ttgggcttgg tattggtctc acgccaagtg gcgatgattt
    17461 tctatgcggt tttattgcca catttcaata ttttactcaa actcagacag cattttacca
    17521 aacactaacc caaaaactcc gacaacattt agataatact aatgcgatta gtcgccgttt
    17581 tttagattgt gcattgcaac aacaattttc cgcccctatt ttgagcttct ttaatcacac
    17641 acaacaacgt caagaggatt tctgcctatt agcacaacgt tttgaaaata tagggcattc
    17701 ttccggaatg gatacacttt tcgggatata ccatgcttgt aaatggctat taaaaaaatc
    17761 catcatcacc tgaatgtgtt tggctttttg acgtaataaa gcagttgaat ttaaaaatgt
    17821 gacgagcaac tagcgttatt taggcagaaa aataccgctt gtagtaatat gcaagcggtt
    17881 gaatttaggg aaaaatttgt aaaatgcttt ttctatatag gttttctgag tttaaggatt
    17941 aattctaagt agttctatcc gatataacgc agctttctta gctctactgc cctcttcttg
    18001 gtaacctagg tgagccggaa tattaaactc atatacagag cctaccggca taagcaataa
    18061 tccttgctgt aagccggaaa acatttctct gataggcaaa attactggca cttgattaaa
    18121 cgtcccttca aacacttccc ctttatcatt ataagaagta aaacgcacct ccacttcatc
    18181 atttagtgtt ggttttttgc cgctgcctgc tttaagctcc ttatattgca agccttgctt
    18241 agtagtttga tacgcttgat ttgcctgtac tttagtagat aataaactta cactagcgac
    18301 aatgaatgcc aaacctaaag cataaatctg cttcattcta tttcccccat cttctataaa
    18361 agatttcata ttatccctac catagacagc tattttttgc gttttatcat ttaattcaaa
    18421 aattaaatta aatctatttg ctattttcag cgattttcgg catatttaga cctaatttaa
    18481 tttatgttta ccataggaat tttttatgaa ccagcaatat tcaacaattc agcataatat
    18541 ccatatgtta ggtgaatttt tgggagaaac gattcgtgat gcgcaaggaa attacattct
    18601 agaactcatc gaaagcattc gtgtgttatc tcgcaattct cgtgccggcg atgaaaacgc
    18661 ccgtgaacag ctattagata aattagccaa tatttctaac gatgatgtct tacccgttgc
    18721 acgtgcattt agccattttc taaatctaac aaatattgct gaacaacatc aaactgtttc
    18781 tcgccatcat attgatgaat cacttggtag tcgctcatta gactctctgt tccttcgtct
    18841 aaaatcacaa aatatccccg ttgaaaaagt gattaaaacc gtggaaagcc taatgattga
    18901 tttagtgcta acagctcacc caacggaagt aacccgccgt tcgctggggc ataaacacgt
    18961 tgaaatcaat aaatgtttga atttgttgga acatgatgat ttaactgagg cagaaagcta
    19021 taaaatcaag cgccgtttaa tgcaactgat tgcccttgct tggcatacta atgaaatcag
    19081 aacccaacgc ccaacccctg ttgatgaagc caaaggcggt atggcagtca ttgaaaatag
    19141 tttatggaaa gccgtgccgg aattttgccg tcagcttaac ttctatttag aaaaacactt
    19201 tggtgtgcaa tatccggtaa atttagcccc tgtccgtttt tcttcttgga tgggcggaga
    19261 ccgtgacggt aacccgttcg tcacggcaga aacaactcgc cgtgtattaa gaatgaaccg
    19321 ttggaaagcc acggaattgt tcttacaaga tattaaaaat cttgctgaag aattatcgat
    19381 tacacagtgt acccctgcat ttcgtgctaa atatggcaac catttagagc catatcgcgt
    19441 attactaaaa gtattacgcc agaaattaat taataccgaa atttactatg cggaattatt
    19501 ggaaggtaaa atttcaacta ttgatgaaag tgacatttta acttcagatc agcaattgtg
    19561 ggaaccgctt tttgattgct atgagtcatt acaagcctgt ggtatgcgaa ttattgcaaa
    19621 tggtgcatta ctagattgct tacgtcgtat aaactgtttt ggtttaggtt tatcacgctt
    19681 agatattcgc caagaaagta cccgccacga aatggcgatt gcagaaatta cccgctatat
    19741 cggcttaggc gattatgctc actggacgga agatgataaa caggcattct tagtgcgtga
    19801 actcagttct cgccgcccat tgattccaac caactggact ccaagccctg aaactaaaga
    19861 agtgttagac acttgtaaaa ttgttgccga gcaacctgaa ggtgtcattt cagcctatgt
    19921 catttcaatg gcaagagagg cctctgatgt actctcggtt caccttttac tcaaagaggc
    19981 ggggtgcaaa tttactattc cggttgcccc attatttgaa acgctaggcg atttagatca
    20041 tagtgaaaaa gtgatgaccg atttatttaa tatcggctgg tatcgtggcg tcatcaacaa
    20101 ttaccaaatg attatgatcg gctactctga ttccgccaaa gatgccggaa tgcttgctgc
    20161 cggctgggcg caatatcggg cgcaagaagc tctggttaat ttatgtgaaa aatataatat
    20221 tgaattaacc ttattccacg gtcgtggtgg tactatcggg cgtggtggcg caccggctca
    20281 cgcagcctta ctttctcagc cacctcgctc actcaaaaat ggcttacgag tgaccgaaca
    20341 aggagaaatg atccgcttta aattaggctt acctgccgtg gcagttgaaa gtttagaact
    20401 ctatgccagt gcgattttag aagcgaatat tttcccacca ccggagccaa agcagcaatg
    20461 gcgagatgtg ctagcaaaag cctccgatat ttcttaccaa atttaccgtg atatggtgcg
    20521 tggcgagcta gacttcgtac cgtatttccg ctctgctaca ccagagcaag aactttcccg
    20581 tctgccgctt ggctcacgcc ctgcaaaacg taatccaaaa ggcggagtgg atagtttgcg
    20641 tgcaattcct tggattttcg cttggatgca aaaccgctta atgctacctg cttggttagg
    20701 tgcagggcaa gcattgcaac agttgattga tgaaggtaat gaaaccttac taaaagaaat
    20761 gtgtaacgaa tggccgtttt tctcgacccg tatcggaatg ctggaaatgg tatttagtaa
    20821 agctgatatt tggctggcgg aatattacga ccaacgccta gtcgagccaa aactacaccg
    20881 tttaggcaat atgttgcgta cgcaactaca ggctgatctt aaaaccgtac ttgcactggc
    20941 tcacgatgga caattaatgg ccgatttacc ttggatcgca gaatcgattg cactacgcaa
    21001 tatttatacc gatccgctca atctacttca ggtagaattg ttagatagga ttcgcaaaca
    21061 aggtgaaaat gctgatcctg tactagagca agcattgatg atcaccatca caggtattgc
    21121 cgcaggtatg cgaaatacag gttaagtttc gatggcaagc ggttgaattt agcggaagat
    21181 ttgcaaaatt ccaacagaat ttaaccgctt atcagccaaa tataaaaaaa tcggggcaaa
    21241 tatttttcgc cccgatttgt atttttactt atttgttttt actttgttaa ttaatgccat
    21301 acggctcggt gtaaggaata atacctcgtt ttagcatacg ggttacacca ttgctgtagt
    21361 ttgtgccaaa gcgtagctca aagttaatgc cgaatttatt gtcatagtag aaatcattga
    21421 tcgtatcaga tgaacctacc ggcgttgaaa ctaagcggcg agccacatac acatttgccg
    21481 cccaacagca agtgttgtag ttaagcgaaa tttgcccttc caccggtttt ttcaatgcaa
    21541 ggtcgtggta ataactggtc attaccgaca ctttatcggt taattcccaa cctagcacac
    21601 cgcccacttg tttaatggat tgcccatact tattcgtgtt caagttttga ttgatgtaat
    21661 ctttacttgc atagcggtaa ttaagctgaa tcaatttgtc tgactgtggt ttgtattgca
    21721 atgacatatt cgctaacgcc gtttggttta aacgagtatc atattgatag ctaccatgcc
    21781 aattccaatt cttatggaat ttccagttag attccgctgc ccatgaagtc gtatgacctt
    21841 tagtgctgtt atctgaggta tcatcaattt tagacgggct tatataataa gtttgtccta
    21901 agctgaaatt aaacacctct tcaccggttg cctctttaaa gaaacgggtt gtgccacctg
    21961 cagtgactaa atttgctgac gaaatacgat ctaaaccgct ataacgtctg tcattaaaca
    22021 gtgagaaata gtcgttttgc aataatgcag agtcatagcc taagctcaca cttcgatgtg
    22081 ctttcgagcc aatatcgctt tgatccttat atgggcgata aatatattgc acccgaggct
    22141 caagcgtttg agtatagcca gagaaaagct gtttatcgga ttccaacacg gttttaaaat
    22201 cgagcttaat ctgtggcagt acacgactta cacttttctt aatttcttcc gtactctcac
    22261 tacctttcgt ttgttgatag taggttgcat agagcttggt ttctaaattt aagctaccat
    22321 agcggttcgc taatgggaaa ttcagtgttg gctcagcgtg gaaacgccac gcttttggca
    22381 ttaatttact atcattttca aaacgggctg cttgggcaaa gaatttgaaa tctccgcctt
    22441 taattagatc gtttttataa tagttaaaat caatttgtgg cagaacgcga taaggcccga
    22501 tatccaactc atcgaaggtt tggaattttt tacctgaaat tgagatatta taattcggct
    22561 gatagtagcc taatttaaaa ttctgcgtcg catagccatc agtactgcta ccataatccg
    22621 aatcaaaatc agagaaataa cgtctgtcgc tcactttggt gtaatcgacc gagaaacgcc
    22681 aatcggttaa aaaactcatt gcgtgacgcc agtggaataa atagcgttta cgatcatcat
    22741 ttcccggttg gtactcgctt aaacgatctt tcgccatata ttcggttgcc actaagcctt
    22801 gcccgatttt cgttaaataa cgaaattccg ggctgatttg ccaaccacgg cgtgaatagt
    22861 aagttggagt gatggtcgag tccatattcg gtgcgatatt ccaataaaac ggttgcgaat
    22921 atgtccagcc gtttttgctt gaacgctcaa aacttggaat taataaccca ctacggcggc
    22981 ggtcgccaat cggaaattgt aagtaaggcg aataaaatac cggcattccc agcactttaa
    23041 aacgggcgtg ccacatttca gcatattctt catcaacgtg ctgaatcatt tcattcgctt
    23101 caatttgcca cgaattatca tttggtaagc acgaagtgaa actggcgttt ttcaacgtac
    23161 gtttgctgtt gccaaactcg ccggattctg ccgtgccacg cccttgtctg ccgactaatt
    23221 ggtagttggc gttagttaaa ttcgcatctt tagatagcaa attaatagtc gcatccgcac
    23281 cggttgcctg aatcaaatta tcttggtagt taaaattacc ctgcaaatac gccatacggg
    23341 cattttcgcc atcttgctca acccgcacct gctctgccca aatcgagcgg ttgccttgct
    23401 tgatggtcac atcaccggtg taggtggcat cacgcggttg gttaatcacc gcactgtctg
    23461 actcaatggt aacaggcatt ctaagttgat caccttctac cacttcgccc tgaaaatgcg
    23521 gcacgccaag taaacattgt tgctttaaat cagctgcggc actgttgcta taacaagctg
    23581 ccatcacagc aagagaaagt aagctataac gctgtttcat catttttcct ttataagccg
    23641 ggaacaagcg gtctgttttt gcaaattttt taccaaaatc gaccgcttgc tcattaaata
    23701 atacgggaaa actgctgtct tctcgctaat tgtcttgaat aaacatcaaa acataagcaa
    23761 atattacgga tcaataaacg ccctcgtgga gaaaccacaa tgctgtcgtc attcatcgtt
    23821 aataattcat cttccgccaa cggcgctaag aaagctaaat cttcagcaaa atagcgttta
    23881 aaatcaatgc catattgctt ttcgatagta gcaaaatcta atttgaaatt acaaatcaag
    23941 gcttttatca catcacggcg aatgcaatca tcttggctta aagcaatgcc tttatgcaac
    24001 gcaatgccgt tggtttcaac atcagcatag tattgttgta attctttgcg gttttgagcg
    24061 taagtatcgc cgagtaagct gattgccgat acgcccattc ccaataaatc acactcttct
    24121 tgagtggtat agccttggaa gttacggtgt aaaatgcctt tttcttgtgc aatggctaac
    24181 tcgtcatcag gtttggcaaa gtgatccata ccgataaact tatagccatt tttgcctaaa
    24241 aattcgatgg aattttgcaa aatggtcaat ttagtttccg gagccggcaa caactcttct
    24301 ttaattttca cttgtgcagc aaaacggctt ggcaagtgag catagttaaa tacgctcata
    24361 cgatcagggt ttaactctac tacacgttgc aaggtgtaca taaagctttc cacattctgt
    24421 tttggtaagc cgtaaatcaa atccacatta gttgaaacaa aaccaagttc ttttgcccgt
    24481 ttcatcaagg caaaaataaa ctcttcatct tgctcacgat tcaccaaacg ttgcacttct
    24541 ttatcgaaat cctgaatacc catactcaga cggttaaaac cgatagcacg caggtgatcc
    24601 aacatactta attcgatttc acgcggatcc atttcaatgc tgatttccgc atcatcgctc
    24661 acatccaaat tggctcgtag catttgcatt aaacgtgccg attgatcttc gtctaaataa
    24721 gtcggcgtac cgccgcccca gtgtatttgg gtcactaaac gattttgaaa cagaggtgcg
    24781 cgagttttaa tttcacgctc taaataatct aaataaattt caactttatg gcgatgacga
    24841 gtaatcactt tattacagcc acagaaatag caaagtttat ggcaaaacgg aatatgcaca
    24901 taaagcgaaa gcggacgctc agggtaacga gctgcagcac gaataaaatc ttcattggtg
    24961 taattttcgt taaactccaa cgctgttggg taagaggtat aacgaggccc tgaatggtta
    25021 tatttttgaa taagggctaa atcccaaata atttctgaca ttagattgcc tttctcattt
    25081 cttcaatatc ttttaataat tggtaacact catttttgat gtctgattca ag
//
LOCUS       DANKBV010000022        24475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.022, whole genome shotgun sequence.
ACCESSION   DANKBV010000022 DANKBV010000000
VERSION     DANKBV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24475
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.022"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(128..889)
                     /locus_tag="PWY43_000696"
     CDS             complement(128..889)
                     /locus_tag="PWY43_000696"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL2135597.1"
                     /translation="MKLNQAQIQNALICGQAVVFDEIDSTNEYLLTHHQRLENGTVCL
                     AEKQTAGRGRRGRTWYSPESENLYFSILWHYPMEQAADLPPLSLVVALIIAESLTAQN
                     VKDIQIKWPNDIYYQGKKMGGILLETQANRNGLDVVIGIGLNLGMAKVDENIVTQAWA
                     DLSQYHFNRNKLVCRLAYELQKNLKIYPLVGFAHYAERWQSFDLFRHKAVKLITEAEE
                     IHGISQGINEQGELILKQGDNLRYFGIGEISLRAD"
     gene            complement(901..1932)
                     /gene="murB"
                     /locus_tag="PWY43_000697"
     CDS             complement(901..1932)
                     /gene="murB"
                     /locus_tag="PWY43_000697"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL2135598.1"
                     /translation="MMKHSLTPFHTFHLPAKATQIIEFTTVEQLLSEWQKAFNAQLPI
                     LILGQGSNVLFLEDFDGVVLVNKLKGITHHEDEHFHYLHVQGGENWHELVKWTLAHNI
                     AGIENLALIPGVAGSAPIQNIGAYGVEFEQLCDFVEVLNLRSGERFKLSKSECEFGYR
                     ESVFKHQYRDEFAIISVGLKLAKAWKPVLSYGSLAQLNPETVTPKQIFDEVCAVRSSK
                     LPNPDEFGNAGSFFKNPVISNEQFAPIQTKFPTIPHYPQANGSVKLAAGWLIDQTGLK
                     GLQIGGAAVHTQQALVLINKENATGQDVLALAKTVRQRVREKFGVEIQPEVRFIGKNG
                     EVDSEKITR"
     gene            complement(2036..2710)
                     /gene="crp"
                     /locus_tag="PWY43_000698"
     CDS             complement(2036..2710)
                     /gene="crp"
                     /locus_tag="PWY43_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDL2135599.1"
                     /translation="MQDMTLSTAPLENVTPPPSIHDPAVEWFLTHCHIHRYPAKYTLI
                     HAGEDAESLFYIVNGSVSVYIKDDETKEMLLTNLGTGEFVGELSLFEEKVQQRTAYVR
                     TKDPCEIAEISYKKFKQLVHLNPDILMYLSAQMARRLRQTSRQVGNLAFLDVAGRIAQ
                     TLLNLAKMPDAMTHPEGMQIKITRQEIGQMVGCSRETVGRIMKMLEDEGLISAHGKTI
                     VVYGAR"
     gene            complement(2772..2990)
                     /locus_tag="PWY43_000699"
     CDS             complement(2772..2990)
                     /locus_tag="PWY43_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDL2135600.1"
                     /translation="MIIPWQELEESTLHNILDSFILREGTDYGERELSLAEKRERLLT
                     QLKADKVVIVWSELHESLDIKDKKSFLG"
     gene            complement(2993..3598)
                     /gene="slmA"
                     /locus_tag="PWY43_000700"
     CDS             complement(2993..3598)
                     /gene="slmA"
                     /locus_tag="PWY43_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDL2135601.1"
                     /translation="MTEPKIKMPKKSVVERRQQVLEVLISLLNSEEGMQRITTERLAT
                     AVGVSEGALYRYFPSKTKMFEALIEKIEQTLTSYIHANKRQDSSANSVRAILYVILEF
                     ARKNPGVTRILTGHALMFEEELLKARVAKFFDNLELQFANILQLSKLRERKSFEDERA
                     LAGYLVNFCEGQFLRLVRSNFSYNQHQHFEKQWAFIKPLFD"
     gene            complement(3610..4065)
                     /gene="dut"
                     /locus_tag="PWY43_000701"
     CDS             complement(3610..4065)
                     /gene="dut"
                     /locus_tag="PWY43_000701"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL2135602.1"
                     /translation="MKQIDLKILDSRIGNEFPLPAYATEGSAGLDLRAMLNEPLTVKA
                     GETVLIPTGISIYIADPNLAAVILPRSGLGHKNGIVLGNLIGLIDSDYQGELKVSLWN
                     RSQADFTVNVGDRIAQLVFVPVVQASFNIVQEFEQTDRGEGGFGHSGKQ"
     gene            complement(4099..4938)
                     /locus_tag="PWY43_000702"
     CDS             complement(4099..4938)
                     /locus_tag="PWY43_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2135603.1"
                     /translation="MKKYNIVFSADKNYLPYLEIALKSLLAHHNHLSIFILSTGDISH
                     HWIETLRPYLENRYSTLRLGYLDSNNLANFNSNGYITNSTYLRYYIEELFEHSDSPYW
                     IYLDCDLVVNGNITDPFTNLDFSFGSIAAIADPYVNKLKNHPYIYKNYFNAGVFYLNA
                     KHFSKQPFIQLTERFKENLPFGDQDILNYCFQYNWIELDRKYNYQTEHLLDDIAQQKI
                     SPNYIPHIIHFTGGIKPLEGKFNSSYVENVASLFRFYHNTSWEILTELPLGTIQLKPF
                     ITE"
     gene            complement(4925..5107)
                     /locus_tag="PWY43_000703"
     CDS             complement(4925..5107)
                     /locus_tag="PWY43_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL2135604.1"
                     /translation="MKIMQLTSNQYINDIKHILLSARQKAYQAVNSAMVEAYWKIGER
                     IVQEEKNMVKDQNEKV"
     gene            complement(5104..5421)
                     /locus_tag="PWY43_000704"
     CDS             complement(5104..5421)
                     /locus_tag="PWY43_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135605.1"
                     /translation="MKLLVSHFHQGEGKFPTFSAGTAVRLGSECVNYPNWFACEIDGW
                     KTFVPSHFISEDKLICDYNPTELVAAQGERVEFLELHYQWAIVKRENEIGWLPCEILR
                     VIG"
     gene            complement(5421..6623)
                     /gene="coaBC"
                     /locus_tag="PWY43_000705"
     CDS             complement(5421..6623)
                     /gene="coaBC"
                     /locus_tag="PWY43_000705"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDL2135606.1"
                     /translation="MLQNKKILVGISGGIAAYKTIEFIRHLKKANAEVRVVLTPAAEA
                     FVTPLTLQAISGNAVSTSLLDPQAELAMGHIELAKWADLIVIAPASADFIARLRMGMG
                     NDLLSTLCLATASPILLAPAMNQQMYKQIAVQENLSVLVNRGVQLIGPNSGFQACGDV
                     GAGRMSEPEEIYQAVLAHFSNNQDLEGLNVTITAGPTREAIDPVRYISNHSSGKMGYA
                     IANAFAERGAKVTLISGPVNLPTPKNVERINVESAVEMEQQAVKFAQKSAIFIGCAAV
                     ADYRMAAVSDQKIKKTDANDELTLKLVKNPDIIANVANLAENRPFVVGFAAETQNVAE
                     YAKSKLERKNLDMICANDVSGGQVFGQDQNALHLFWKNGEKTLPLADKSKLAESLVQE
                     IVKHYREK"
     gene            6773..7510
                     /gene="radC"
                     /locus_tag="PWY43_000706"
     CDS             6773..7510
                     /gene="radC"
                     /locus_tag="PWY43_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL2135607.1"
                     /translation="MEEKMNDFFDEDSQEPTMMPREKLLTQGAASLTDAELLAIFLRT
                     GTKAVPVLALAESVLMAFGSLRQLLNANIDDFCKIYGLGKTKYIQLQASKEMTKRYLA
                     QQMEFSEMIQAPYMAIMYFQTELEEEEREVFMVLFLDNQNRLIYKEKMFFGTINQTAV
                     HPREIIKRALKYNAAAIIVAHNHPSGSCLPSESDRSLTKKIEMACELVDIRFVDHIIV
                     GKGDYFSFEEEKLEMKEHSFLQISDRK"
     gene            7741..7977
                     /gene="rpmB"
                     /locus_tag="PWY43_000707"
     CDS             7741..7977
                     /gene="rpmB"
                     /locus_tag="PWY43_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL2135608.1"
                     /translation="MSRVCQVTGKRPAVGNNRSHAMNATKRRFLPNLHTHRFWVESEN
                     RFVTLRLTAKGMRIIDKKGIDAVLAEIRARGEKI"
     gene            7989..8159
                     /gene="rpmG"
                     /locus_tag="PWY43_000708"
     CDS             7989..8159
                     /gene="rpmG"
                     /locus_tag="PWY43_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2135609.1"
                     /translation="MAAKGAREKIRLVSTAETGHFYTTDKNKRNMPEKMEIKKFDPVV
                     RKHVIYKEAKIK"
     gene            complement(8240..8995)
                     /locus_tag="PWY43_000709"
     CDS             complement(8240..8995)
                     /locus_tag="PWY43_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2135610.1"
                     /translation="MSKNRLEQILLFLKNHNIATVEQLVKVTEASPATIRRDLIKLDE
                     EGVVIRTHGGVSLNQFIPFQPTTNEKQFKQIVEKERIAEYAVSLISPGNSILLDAGTT
                     TLCIAKKIAHMPLRVITTDLHIALLLSEYKQIELIITGGRVDNSSQSCIGNHSLTLLE
                     NINPDIAFVSCNSWSLEKGVTAPTEEKANLKRMLQQNAKQKVLVADSSKYGKYSLFRA
                     TQLDNFTQIITDKGLPIEYSQALSSNDFKLSLV"
     gene            complement(9103..10309)
                     /locus_tag="PWY43_000710"
                     /pseudo
     CDS             complement(9103..10309)
                     /locus_tag="PWY43_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(10306..11304)
                     /locus_tag="PWY43_000711"
     CDS             complement(10306..11304)
                     /locus_tag="PWY43_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDL2135611.1"
                     /translation="MSTQTSKSIVPIYDGMNKIPGGAMVIPLILGSIIGTFFPEFLNL
                     GSFTTALFKSSAGPLIALLIFATGMQITVRSSGPVLAHTGVVLLFKTIIPALLVVILG
                     LWVGNDGVMGVSLLAMLTVVANSNGGIWLAFTGKYGDYRDKGAYVASAVNDGPFFVLL
                     FLGVSGLADIPLSLMLAAVIPLIIGMVISNLDPKWTELMKPTGAIVIPFFAFALGTGI
                     NLQSIITGGATGIILGIVASLFTGFLVFIGYKTILRRGNQSGIGFAAGTTAGNAIITP
                     EIVAQADPTLAPFVQTATAQIAAAVLVSAILAPLFAAWVLKRQGGLEQDTIKGENL"
     gene            complement(11315..12316)
                     /gene="pdxA"
                     /locus_tag="PWY43_000712"
     CDS             complement(11315..12316)
                     /gene="pdxA"
                     /locus_tag="PWY43_000712"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDL2135612.1"
                     /translation="MKPILGITMGDAAGIGPEVIIKALANQRIYELAHPVVIGDRKII
                     QRALPIVHSELKLRILNNLDNFVAEYGVIDILDLNNLPEDLPFAQVDPRAGKAAYEYI
                     EEAVNLAMGGKIHAIVTAPLNKEALHAGGKPFPGHTEILATLSNTKDYSMMLTSEKLN
                     VIHVTTHVQLRKACDLITKERVATVIDLAEENARILGFKEPRIAVAGLNPHSGENGMF
                     GDEDQKEIVPAIQEAQAKGFNVTGPVPPDTLFHRAANLNEFDIVVVMYHDQGHIPIKL
                     LGFDTGVNVTVGLPFIRTSVDHGTAFPIAGKGTADSRSMTEALYLGAKMANIKYATH"
     gene            12750..14114
                     /locus_tag="PWY43_000713"
     CDS             12750..14114
                     /locus_tag="PWY43_000713"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDL2135613.1"
                     /translation="MTIKTPIQAVLASNQYFLDRQDAMESNVRSYPRKLPFAYQKAQG
                     VWVTDVEGNEYLDFLAGAGTLALGHNHPKLIQAVKDVLDSGLPLHSLDITTPLKDAFT
                     EELLSFFPKDKYILQFTGPSGADANEAAIKLAKTYTGRGNVIAFSGGFHGMTHGALSL
                     TGNLSAKNAVQGLMPGVQFMPYPHEYRCPFGIGGEAGAKAVEHYFENFIEDVESGVVK
                     PAAVILEAIQGEGGVVPAPISFLQKVREVTQKHGILMIVDEVQAGFCRSGKMFAFEHA
                     GIEPDIVVMSKAIGGSLPLAVLAIKKEFDAWQPAGHTGTFRGNQLAMATGYVSLKIMC
                     EENLAKNAQERGEYLTNALRELSKELPCIGNVRGKGLMMGIDIVDERQPKDATGAYPR
                     DCDLAVAIQKYCFKNKLLLERGGRGGNVVRVLCAVNITQAECEEFIKRFKQSVAEAVK
                     AVRG"
     gene            14204..15739
                     /gene="ddc"
                     /locus_tag="PWY43_000714"
     CDS             14204..15739
                     /gene="ddc"
                     /locus_tag="PWY43_000714"
                     /EC_number="4.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,4-diaminobutyrate decarboxylase"
                     /protein_id="HDL2135614.1"
                     /translation="MSNLEKHRQSLFCSDPKSIADYESAMNNAVKAVSDWLKEEKMYT
                     GGSIKELRAKINGFNPTQAGVGVQKSQEHLVEIFLKPSLKVHHPHSLAHLHCPTMVTS
                     QIAEVLINATNQSMDSWDQSPAGSIMEEHLIDWLRQKVGYGKGTSGVFTSGGTQSNLM
                     GVLLARDWAIANHWKKADGSEWSVQADGIPADAMQKVKVVCSENAHFSVQKNMAMMGM
                     GFQSVVTVPSNANAQMDVEALAKTLADLKAEGKIVACIVATAGTTDAGAIDDLKAIRK
                     LADEHQAWVHVDAAWGGALLLSKDFRHFLDGIELTDSITLDFHKHFFQSISCGAFLLK
                     DEANYRFIDYKADYLNSEYDEEHGVPNLVSKSLQTTRRFDALKLWFTVEALGEELYAS
                     MIDHGVYLSKEVEAYINATDGLEMLVPAQFASVLFRVVPQGYPAEFVDTLNQNVADEL
                     FARGEANIGVTKVGEHQSLKMTTLSPIATLENVRALLAQVLAEADRIKDSIVNGTYVP
                     PID"
     gene            15842..16210
                     /locus_tag="PWY43_000715"
     CDS             15842..16210
                     /locus_tag="PWY43_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HDL2135615.1"
                     /translation="MKKVLGLTLMAMALSACNSTASNGSSQANTGVAPANVISVKQAL
                     AAADDSYVTVEGTLLNKVGDETYLFKDATGQIRVEIDDEVWAGQHVGSKGNKVRLYGE
                     IDKGFNKTELDVKTLTVIKP"
     gene            complement(16285..16872)
                     /locus_tag="PWY43_000716"
     CDS             complement(16285..16872)
                     /locus_tag="PWY43_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2135616.1"
                     /translation="MNHLIIYAHPNPSSFNHAILEKTIAASVPNKVVVRDLYQLNFKP
                     ILDWAEFQGTLSKHYEADVAIEHQYWLEADVITLIYPLWWMGFPAILKGYLDRVLSYD
                     FAYRNGEIESIGLLTGKKMQQFVTLGNSNEKYERKGFLAAFEHTLGKGLFEFCGIRDV
                     KMHYFGNIGLKETDYTAILQQITASCSTILKEQAV"
     gene            17048..17596
                     /gene="napF"
                     /locus_tag="PWY43_000717"
     CDS             17048..17596
                     /gene="napF"
                     /locus_tag="PWY43_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDL2135617.1"
                     /translation="MTDYPNQSLPRRSFLRGQFLDSLKSQKVSQQGYEPIRPPWANLA
                     NFLEKCTACGNCIQACETQIIVKGAAGYPEIDFSRGECTFCEKCVHSCEAEVFRAVSE
                     QPWSHKIEIQAGCLLKLGTECRSCGDSCEMRVIRFRPSLGGIAEMVLNLESCNGCGAC
                     LSVCPTSAIKIQPLQTQANRDE"
     gene            17589..17855
                     /locus_tag="PWY43_000718"
     CDS             17589..17855
                     /locus_tag="PWY43_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDL2135618.1"
                     /translation="MSEKLSEQENWYVCSLVVQAKPEKLEEVKAEILKIPHTEIHGEK
                     TDEGKLVVTIESNVHLALSDRIDQVRDIKGVIVVSLISNYIDEQ"
     gene            17894..20392
                     /gene="napA"
                     /locus_tag="PWY43_000719"
     CDS             17894..20392
                     /gene="napA"
                     /locus_tag="PWY43_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDL2135619.1"
                     /translation="MELNRRDFMKANAAVAAAAAAGIMIPVKNVQADDTGIRWDKAPC
                     RYCGTGCSVLVGTKDGKVVATQGDPDAEVNRGLNCIKGYFLSKMMYGADRMQEPMLRM
                     KDGKFDKNGDFTPVSWDQAFSIMAEKIKTTIKNDGPNAVGMFSSGQTTIFEGYAKVKL
                     WKGGFRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMH
                     PILWSRISDRRLSDNKVKVVVMSTYEHRSFELADTPIIFKPHSDLAILNYIANYIIQN
                     DKVNWDFVNKHTKFKRGETNIGYGLRPEHKLQQNTNAKTAGKMYDSDFEEFKKIVAPY
                     TLEEVHRISGVPKDQLETLAKLYADPNQKLVSFWTMGFNQHTRGVWVNHMMYNVHLLT
                     GKIALEGCGPFSLTGQPSACGTAREVGTFIHRLPADMVVTNPKHREIVEKSWKLPAGA
                     IPDVPGFHATAQSRALKDGKMKFLWQMCTNNMQGGPNINEEIFPGWRNPETFIVVSDP
                     YPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPANARSDTWQLVEFSKY
                     FKVEEVWPEELLAKAPELRGKTLYEILYRNGKVDSYQVPTNIPGYMNDEAEHFGYYIQ
                     KGLFEEYAEFGRGHGHDLADFDTYHKVRGLRWPVVKDEKTGEYKETLWRYREGYDPYV
                     KAGEEVAFYGNADKKAVILGVPYEPPAEAPDEEYDLWFCTGRVLEHWHTGTMTRRVPE
                     LHKAFPTNLVWMHPNDAKKRGLRHGDKVKVISRRGEYVSNLDTRGRNKCPEGLIYSTF
                     FDAGQLINKVTLDATDPISFETDFKKCAVKVVKA"
     gene            20481..21386
                     /gene="napG"
                     /locus_tag="PWY43_000720"
     CDS             20481..21386
                     /gene="napG"
                     /locus_tag="PWY43_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDL2135620.1"
                     /translation="MKMDPNRRQFLKNATRTAAGVCGVGIILGLQQQQSFARQGVALR
                     PPGALPEQDFLSACTRCGQCVQACPYDMLHLASLISPVEAGTPYFIARDKPCEMCVDI
                     PCMNACPTGALSEELKDIDDARMGLAVLLDHETCLNWQGLRCDVCYRVCPLIDKAITL
                     EKVHNDRTSIHAKLIPTVHSDACTGCGKCEQACVLEEAAIKVLPMDLAKGLLGRHYRL
                     GWQEKQNAGKSLIEDVHPEGLRPAYDARMPEGLHEPVYQHMEVKPDVKVATPSRATQN
                     YVPNSTTVEAPEHFPDLDLNLKGVK"
     gene            21386..22264
                     /gene="napH"
                     /locus_tag="PWY43_000721"
     CDS             21386..22264
                     /gene="napH"
                     /locus_tag="PWY43_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDL2135621.1"
                     /translation="MAANSPKNAGLEARQKLGWWHAYRFLILRRLSQLSIILMFLSGP
                     LWNVWILKGNYSGSLLLDTVPMTDPLMTAEVLATGYRPEWTVLVGALIMVAFYGIVGS
                     KLFCSWVCPMNMVTDTAAWLRRKLGIRQTAKIPRNLRYVILAVILVGSAISGTLLWEW
                     INPVAALGRVFVFGLGAMLWLVLVVFLFDLLVAEHGWCGHLCPIGATYALIGAKSLIK
                     VKVVDRNRCDNCMDCYNVCPEPQVLRVPLHGKPEESTIILSKDCITCGRCIDVCAENV
                     FAFGSRFQGSIDVKNI"
     gene            22310..22738
                     /locus_tag="PWY43_000722"
     CDS             22310..22738
                     /locus_tag="PWY43_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDL2135622.1"
                     /translation="MRKYLALILAVMSGFAMAEAPKLSGTIDETTESVAPGFHNPQKD
                     VGTIPTTFPFQPPLVPHSIRGLQVSKNVNHCLGCHAIEPSKTTGATRLPPSHFTDRDG
                     KVYENESPRRYFCLQCHVQQTDVNPIIQNKFESVRESQAK"
     gene            22749..23357
                     /locus_tag="PWY43_000723"
     CDS             22749..23357
                     /locus_tag="PWY43_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL2135623.1"
                     /translation="MLRRFCNWLRSPSKMAIGAVILISALGGIFAWSGFNAGLAMTNT
                     EEFCSDCHMNDVVPEYRASAHYSNRSGVKAICSDCHVPHEFFPKWQRKIIAAKEVYAH
                     FIGKVDTKEKFEAHRGEMAEREWARMKANGSQECRNCHNFDDMDFTQQKNIAQQMHAL
                     AQEQGKTCIDCHKGIAHNLPHMEAIQKNFIPDDLLKKAEEKK"
     gene            complement(23419..24261)
                     /gene="cpdA"
                     /locus_tag="PWY43_000724"
     CDS             complement(23419..24261)
                     /gene="cpdA"
                     /locus_tag="PWY43_000724"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDL2135624.1"
                     /translation="MDAKRMHEYIKLPVTGVIKLLQITDPHLFSNPEETLLNVKTVKS
                     FSAVIEQINKQAKQYFDLVLATGDLIQDHNIAGYHYFAQITNSLNSPIVWLEGNHDVQ
                     PSMSEILAQYKHILPHKQILLGEQWQLLMLNTQVVGMPYGELSYSQLKWLEEKLAQYP
                     QRFTLVALHHNILPTNSAWLDQHSLKNSNELSEIFAKFKNVKAIVHGHIHQQVDDIWH
                     GIPIYATPSTCIQFKPNCDQFTLDLQPQGWREIELHENGTIETVVKRLNSNDFLPNVN
                     AIGY"
BASE COUNT         7178 a         4874 c         5175 g         7248 t
ORIGIN      
        1 gggcaatatc tctcaagcct gcaatgtggt gggtatgagc ctagatacat tctattgcta
       61 tcaacaagcg gttatttttg gcaactaatt tgcaaaaaaa caataaaatc cgaccgcttg
      121 tagagggtta atccgccctt agtgaaattt caccgatgcc gaaatagcgg aggttgtcgc
      181 cctgttttag aatgagttcg ccctgctcgt taatgccttg cgaaatgccg tggatttctt
      241 cggcttcggt aatgagctta accgctttat ggcggaaaag gtcgaaactt tgccagcgtt
      301 cggcataatg agcgaagccg acaagcggat aaattttgag atttttttgc aattcatagg
      361 ctaaacggca aaccaatttg ttgcggttga aatgatattg cgataaatct gcccacgctt
      421 gcgtcacaat attttcatcc acttttgcca ttcctaggtt cagcccaatg ccgataacca
      481 catctaaacc attgcgattg gcttgggttt ccaacaaaat gccccccatt tttttgcctt
      541 ggtaataaat gtcattcggc catttaattt gaatatcttt tacattttga gcagtaaggc
      601 tttccgcaat gatcaaggct accactaaac ttaagggcgg taaatctgcc gcctgctcca
      661 tcgggtaatg ccataaaatc gagaaataga gattttcact ctcgggcgaa taccaagttc
      721 gacctcgtct gccacgtccg gcagtttgtt tttcagcgag gcaaaccgtg ccgttttcca
      781 aacgttggtg atgagtgagc aaatattcat tggttgaatc aatttcatcg aacacaacgg
      841 cttgcccgca aattaacgca ttttggattt gagcttggtt aagtttcatt atgtccccac
      901 ttagcgagtg attttttcgc tatcgacctc gccgttcttg ccgataaaac gcacttcagg
      961 ctgaatttct accccaaatt tttctctcac tctctggcga acggttttgg ctaacgccaa
     1021 aacatcttgc cctgttgcat tttctttgtt aatcagcact aaggcttgct gggtatgtac
     1081 cgccgctccg ccaatttgta agcctttcag cccggtttgg tcaatcaacc aacctgcggc
     1141 taattttacc gaaccattag cttgaggata gtgaggaatg gttgggaatt tggtttgtat
     1201 tggtgcaaat tgttcgtttg aaatcaccgg atttttaaaa aagctgccgg cattgccaaa
     1261 ttcatcaggg ttaggcaatt ttgaggagcg aaccgcacaa acttcatcaa aaatctgttt
     1321 tggcgtaacg gtttccggat tgagttgggc taatgatccg taacttagca ccggtttcca
     1381 cgctttggct agctttaagc ctaccgagat aatcgcaaat tcatcacggt attggtgttt
     1441 aaacacactt tcacggtagc caaattcgca ctcggatttg gatagcttaa agcgttcgcc
     1501 ggaacgtaaa tttaacactt ccacgaaatc acacagctgt tcaaattcca ccccgtaagc
     1561 cccaatattt tgaattggag cagagccggc gacacccgga attaacgcca gattttcaat
     1621 gccggcaata ttgtgtgcca acgtccattt aaccaattcg tgccagtttt ccccaccttg
     1681 aacgtgcaaa tagtggaaat gttcgtcttc gtggtgcgta atccctttga gtttatttac
     1741 taacaccaca ccgtcaaaat cctctaaaaa caaaacgtta gagccttgcc cgagaatcaa
     1801 gattggcagt tgagcgttaa aggctttttg ccattcgctc aatagctgtt cgactgtggt
     1861 aaattcaata atttgggtcg cttttgcagg caaatggaaa gtgtgaaaag gggttaagct
     1921 atgtttcatc atcaaattag ttagttaaga agtggaaata acaagcggtg atatttttac
     1981 aaaattttgc aattaagatt ttgggcaggc acaggatctg cccctacatt cagaattacc
     2041 ttgccccata taccacaatg gttttgccgt gagcagaaat taagccttcg tcttccaaca
     2101 ttttcataat gcgtccaacg gtttcacggg aacaaccgac catttgcccg atttcttggc
     2161 gggtaatttt gatctgcata ccttccgggt gagtcattgc atccggcatt ttagctaagt
     2221 tgagcaaggt ttgagcaatg cgaccggcaa cgtctaaaaa ggcaagatta ccgacttggc
     2281 gtgaagtttg acgtaaacgg cgagccattt gtgccgataa atacattaaa atatcggggt
     2341 ttaaatggac tagttgtttg aattttttat aggaaatttc cgcaatttca caaggatctt
     2401 tagtgcggac gtaagcggta cgttgttgga ctttttcttc aaataaactt aattcaccga
     2461 caaactcacc tgtgcctaag ttagttaaga gcatctcttt ggtttcatca tctttgatgt
     2521 aaaccgacac tgagccattc acgatgtaga aaagtgattc tgcatcttca ccggcgtgaa
     2581 tgagtgtata tttcgccgga taacgatgga tatgacaatg ggttaaaaac cactccaccg
     2641 cagggtcgtg aatagagggc ggaggagtta cattttccag aggagctgtt gaaagtgtca
     2701 tatcttgcat agtatttcct ataactctta tactaggtta aatttttaga attttaacac
     2761 gaaattaaag gttagcctaa aaaagatttt ttgtctttaa tatcaaggct ttcgtgtaat
     2821 tccgaccaaa caatcaccac tttatctgcc ttaagttgtg tgagtaatcg ttctcttttt
     2881 tctgccagag aaagttccct ttccccgtag tctgtgcctt ctcgtaaaat gaaggaatct
     2941 aaaatattat gtaaggttga ttcttcgagt tcttgccaag gtataatcat aattaatcaa
     3001 ataaaggctt aataaatgcc cactgttttt caaaatgttg gtgttggtta tagctgaaat
     3061 tagaacgaac caagcgtaaa aattgccctt cgcagaaatt cactaaataa ccggctaagg
     3121 ctctttcgtc ttcaaagctc ttgcgttcac gcagtttact aagttgcaaa atattggcaa
     3181 attgtaactc caagttatca aagaattttg ccactcgagc tttgagcagc tcctcttcaa
     3241 acatcaaggc gtgtccggtc aggatgcggg taacgcccgg gtttttgcga gcaaattcta
     3301 aaatcacata taaaattgct cgcaccgaat tggcactgct gtcttggcgt ttattggcgt
     3361 gaatatagct ggtgagggtc tgctcgattt tctcgattaa cgcttcaaac atcttggttt
     3421 tacttgggaa atagcgatac aacgccccct ccgacacccc caccgcagtg gctaaacgct
     3481 cggtagtgat acgctgcatt ccctcttcag aatttaacaa gctgattaac acttccaaca
     3541 cttgttggcg gcgttccacc actgattttt tcggcatttt aattttaggt tctgtcattg
     3601 ccaatcctat tattgcttac ctgaatgccc aaaaccgcct tcgccacggt cggtctgctc
     3661 aaactcttgc acaatgttaa aacttgcctg caccaccggc acaaagacta actgagcaat
     3721 acggtcgccc acgttcacgg taaaatcagc ttggctacgg ttccaaagcg acactttcag
     3781 ctcgccttgg taatcgctat cgatcaagcc gattaagttg cctagcacga tcccgttttt
     3841 atgccctaaa cctgatcttg gtaagatcac cgccgccaaa ttcggatcgg cgatataaat
     3901 tgaaatcccc gttgggatca acaccgtttc acccgctttt accgtcagcg gctcgttgag
     3961 catcgcccgt agatccaagc ctgccgagcc ttcggtcgca tacgcaggta atggaaattc
     4021 attgccgata cggctatcta aaatttttaa atcaatttgt ttcattcttt ttcctttttg
     4081 taaattttta aaactatatt attcagttat aaaaggtttt aattgtatcg ttccaagtgg
     4141 aagttcagtc aatatttccc agcttgtgtt gtggtaaaat cgaaataaac ttgcaacgtt
     4201 ttcaacgtag cttgaattaa atttaccttc taatggcttt attcctccag taaaatgaat
     4261 tatatgtgga atataatttg gcgaaatttt ttgctgggca atatcatcta ataaatgttc
     4321 tgtctgataa ttatattttc gatctaactc aatccaatta tattgaaagc agtaattaag
     4381 aatatcttga tccccaaatg gtaaattttc tttaaatctc tcagtaagct gtataaatgg
     4441 ttgcttacta aaatgctttg catttagata aaatacacca gcattgaaat agtttttgta
     4501 aatataggga tgatttttta gtttgtttac ataaggatca gcaattgcag caatacttcc
     4561 aaaagaaaaa tctaggtttg taaatggatc cgtaatattt ccattaacca caagatcgca
     4621 atctaaataa atccaatatg ggctatcgct gtgttcaaat agttcttcaa tataatatcg
     4681 taggtaagtt gaattagtaa tatagccgtt ggagttaaaa tttgctaaat tatttgaatc
     4741 taaatagcca agtcttaatg tggaatatct attttctaaa taggggcgta atgtttctat
     4801 ccagtggtga gaaatatcgc cagtactcaa tataaaaata gataaatgat tatgatgagc
     4861 taatagtgat tttaaggcaa tctctaaata tggcaggtag tttttatctg ctgaaaatac
     4921 aatattatac tttttcattt tgatctttga ccatattttt ctcttcttgc acaattcgtt
     4981 caccgatttt ccaataggct tcgaccattg ccgaattcac ggcttgatac gccttttggc
     5041 gagcagataa aagaatatgt ttaatgtcgt tgatatactg atttgacgta agttgcataa
     5101 ttttcatcct attaccctta agatttcaca aggtagccag ccaatttcgt tttctctttt
     5161 gacaattgcc cattggtaat gtagttcaag gaattctact ctctcgcctt gtgctgcaac
     5221 aagttccgtt gggttataat cgcatatcaa tttatcttcg ctaataaaat ggcttggcac
     5281 aaaggttttc caaccgtcaa tttcacaggc aaaccagttt gggtagttta cacattcact
     5341 ccctaatcga acggcagtac cagctgagaa tgtaggaaat ttcccttcgc cttgatgaaa
     5401 atggctaacc agtaatttca ttatttttct cgataatgtt ttacaatttc ctgtaccaaa
     5461 ctttctgcca atttgctttt atccgccaga ggaagcgttt tttcaccatt tttccagaac
     5521 aaatgcaacg cattttgatc ctgcccgaac acttgtccgc ctgagacatc attggcacaa
     5581 atcatatcca gatttttgcg ttcgagtttc gatttagcgt actccgccac attttgcgtt
     5641 tctgcggcaa aaccaaccac aaaagggcga ttttcggcaa gattggcgac gttagcgata
     5701 atatcggggt tttttaccaa ttttagcgtg agttcatcat tggcatcggt tttcttaatt
     5761 ttttggtcag agactgccgc cattcggtaa tcggcaaccg ctgcacagcc aataaaaatc
     5821 gcagattttt gggcaaattt gaccgcttgt tgctccatct caaccgcaga ttccacatta
     5881 attcgttcca catttttcgg cgttggtaaa ttcaccggac cgctaatcaa cgtaaccttt
     5941 gctccacgtt cggcaaaggc attagcaatc gcgtagccca ttttgccgga gctgtggttg
     6001 ctgatataac gcacagggtc aattgcctca cgagtcggtc cggcggtaat ggttacattt
     6061 aagccctcta aatcttgatt gttggagaaa tgagccaaca ccgcttggta aatttcttca
     6121 ggctcagaca ttctgccagc ccccacatca ccacaggctt ggaagccact attcgggccg
     6181 atgagctgaa cgccacgatt gactaaaaca gacagatttt cttgcaccgc aatctgtttg
     6241 tacatttgtt gattcattgc cggtgcaagc aaaatcgggc ttgcagtcgc aaggcagaga
     6301 gtggaaagta aatcgttgcc cattcccata cgcaggcgag caataaaatc ggcacttgcc
     6361 ggggcgataa cgattaaatc cgcccatttt gccagctcaa tatgccccat cgccagttcg
     6421 gcttgtgggt cgagtagtga agtggaaacc gcattgccgg aaatcgcttg taaggtcaaa
     6481 ggcgttacaa aggcttctgc tgctggggtt agcactacac gcacttccgc atttgccttt
     6541 tttaagtggc ggataaattc aatggttttg taggctgcaa tcccaccgga aatgccgact
     6601 aaaatttttt tgttttgtaa catctttact atccttaaag cgtggattta ttttgcatat
     6661 tttagcggaa taaggagttg ctcacaactt cactattttt gcgatcttgc tcgaaaaatt
     6721 gaataaatcc ctttgtctgc aaaggttggc tcttttatgc ttaactaaat tgatggagga
     6781 aaaaatgaac gatttttttg atgaagattc tcaagagcca acaatgatgc cgcgtgagaa
     6841 attattaaca cagggggcag cctcattaac cgatgccgaa ttattagcga tatttttacg
     6901 gactggtacg aaagctgtgc ctgtgctggc attggcagag agtgtgttga tggcatttgg
     6961 ctcgttacgc caattactga atgcaaatat tgatgatttt tgcaaaattt acggtttagg
     7021 caaaaccaaa tacattcagt tacaagcctc taaagagatg accaaacgct atctggctca
     7081 acaaatggaa tttagcgaga tgattcaagc cccttatatg gcgattatgt atttccaaac
     7141 cgagttggag gaagaagaac gggaagtttt tatggtgctg tttttggaca accaaaaccg
     7201 attgatttat aaagaaaaaa tgttcttcgg caccattaat caaactgctg ttcacccccg
     7261 agaaattatt aaaagagcgt taaaatataa tgccgcggcg attattgtgg ctcataatca
     7321 cccctcaggt tcttgtctgc cgagtgagtc ggacagaagt ttaactaaaa agatcgaaat
     7381 ggcgtgcgaa ttggttgata ttcgctttgt ggatcatatt attgtcggca aaggggacta
     7441 tttttcgttc gaggaagaga aattagagat gaaggagcat tcatttttgc aaatttctga
     7501 tagaaaatga ccgcttgctt tatgtaacct ttaacatttc tcctgaaaca gtaaaagata
     7561 cgcatataaa ttctacttga atataacaaa tgaaatcggt ataatcggca gtctttatta
     7621 catagactgg gtcgggttaa tgcctgacga ggcaatcaaa atcggagggg ttggttgagc
     7681 aacgcctttt gtgagatctc ccgaaccatt tttaagctta aaattattgg agaaattcaa
     7741 atgtcaagag tttgccaagt aaccggcaag cgtccagcag ttggtaacaa ccgctcacac
     7801 gcaatgaatg cgactaaacg tcgtttctta ccaaaccttc acactcaccg tttctgggtt
     7861 gagagcgaaa accgtttcgt aaccttacgt ttaactgcga aaggtatgcg tattattgat
     7921 aaaaaaggca ttgatgcagt attagctgaa atccgtgctc gtggcgaaaa aatctaagga
     7981 gctaaaaaat ggcagctaaa ggcgctcgtg agaaaatccg tttagtttct acagcagaaa
     8041 ctggtcactt ctacacaaca gataaaaaca aacgtaatat gcctgaaaaa atggaaatca
     8101 aaaaatttga tccagttgtg cgtaaacacg ttatctacaa agaagcaaaa attaaataat
     8161 tttgttttaa gatttaaaac ccagcgaatg ctgggttttt catatacaaa aaattagtga
     8221 aaaaagaccg cttgtatgat taaactaatg atagcttaaa atcattactt gacaaggctt
     8281 ggctatattc aataggtagt cctttatctg tgataatttg tgtaaagtta tcgagttgag
     8341 ttgcacgaaa taaagagtat tttccatatt tgctgctatc ggcaaccagt actttttgtt
     8401 tggcgttttg ttggagcatt ctttttaaat tagctttctc ttctgtaggt gctgtaactc
     8461 ctttttctaa gctccaagaa ttacagctca caaaagcaat atcaggatta atattttcta
     8521 ataaagttaa actatgatta ccaatacaag attgactact attatctact cgtccccctg
     8581 taataattaa ttctatttgc ttatattcag agagtaataa agcaatatgt agatcagttg
     8641 taatcactcg aagtggcata tgagcgattt ttttcgcaat gcatagcgtt gtagtgccag
     8701 catctaataa aatagaattt cccggtgaaa taagcgaaac ggcatattca gctatgcgtt
     8761 ctttttcgac aatttgctta aattgcttct catttgttgt tggttgaaac ggtataaatt
     8821 ggtttaagct gacccctccg tgtgtgcgaa tcacgacgcc ttcttcatct aattttatta
     8881 aatcacggcg gatagttgca ggagaagcct cagtcacttt taccagttgc tctacggtag
     8941 caatattgtg gttttttagg aatagtaaaa tttgttctag acggttttta gacatataaa
     9001 ctctctaatt tttaactgct tttactataa gtaatttttt aaaaataaga tagttaaaat
     9061 taaagaacct aaaacattgt tgctttaggt tctttgttgt tactagttca ttatattgct
     9121 taagagtgtg ttgtttccaa atcctccaga tttagtcatt acagtgatgc ctgagtagtt
     9181 gcccttttca atggagcata aaggaacgcc cggtactacc gcatattgta cattcatctc
     9241 tgttacattc atttgatcta ataatgctgc tgcaccatct ccaccaaata atataaggct
     9301 atcaaatttt tcattatcaa ggcagaattt acttaaatca gctaaatgtt gagaaatttc
     9361 tttagaagtt tccgtgatat tggcagattt tgcatttaca acttttgcag ggtttgcccg
     9421 aataataaca tgatctctac taaaacgtat tagagcgttt aattgtagtt tcagtgaatg
     9481 ttctgattgc gagtagttaa tcaactgtga aggcgaagga ttaaatacaa tagattttcc
     9541 tcctaaagtt gaaatatatt catcaacttg cagttggctt gtttcgtgaa ttgaggttac
     9601 gacaattaat gctctaccta atttaagatt tcgctctggt tgttgttgat tttggatatt
     9661 tttcaacttc tgtgccaatc cggctgagcc aacaggaata attttattac ctatgtgatt
     9721 tatgacttgg gcaatcattg agagatcttc gtgcgtttta gcatcaataa cgatctgttt
     9781 atattcacta ttttcaatag atgcctgcaa attatcgacc gtatcacacg ggattacttg
     9841 agcatcaggc aatagttttt gcatagaaga tgaatgcaca gggcagatag gatcttttcc
     9901 tgaagcagtt tcatttacag gaacaccatt aacatataaa tgaccagctt ctatagttct
     9961 tcccatttct ggatatgcag gacaaataat tgctttaaca tcgggatata aaccagccca
    10021 agctttaata gcaccttcaa tctgatattt aacagagcct ctcattgtgg aatcaatttt
    10081 aaggtaaatt tgcccgatac tattttgaat agcattgcta attgcaattc tctccaattg
    10141 ctcttgaatc tgtagaaaca gaaattactt gggctttatc aggatgaatt tgggctaatg
    10201 ccgaaacttt agtttttaag acagtgtcaa atcctgattc agcaaacata accgcggtat
    10261 catttgcacc agtaaggcca tcagcaataa ctaagagtgt ctctttcata gattttctcc
    10321 ttttatagtg tcttgttcta agccaccttg acgttttaaa acccacgctg caaataatgg
    10381 agctaagatt gctgaaacta atacagctgc ggcaatctgt gcagtcgcag tttgaacaaa
    10441 aggtgctaat gtagggtctg cttgagcaac aatttcaggt gtaataatgg catttccagc
    10501 agtagttcct gctgcaaacc cgattcctga ttgattacca cgtcttaaaa ttgttttgta
    10561 accgataaat acaagaaaac ctgtaaagag agaagctacg atacctaaaa taattcctgt
    10621 tgctcctcca gtaataatag attgcagatt gattcctgtt cctaatgcaa aagcaaagaa
    10681 aggaatgact attgcacctg ttggtttcat taactctgtc catttaggat ctaagttact
    10741 aataaccata ccaataataa gaggaataac agctgcaagc attaatgaaa gaggaatatc
    10801 tgctaatcct gatacaccta aaaatagtaa aacaaagaaa ggtccatcat taacagccga
    10861 agcgacataa gctcctttat ctctataatc accatatttc cctgtaaacg ctaaccaaat
    10921 accaccattt gaatttgcaa caacagttag catagctaat aaagaaacgc ccataacacc
    10981 atcattacct acccataatc ctaaaatcac aactaaaaga gcgggaataa tagttttaaa
    11041 tagtaataca acaccggtat gggcaagtac aggtccagaa cttctcacgg taatttgcat
    11101 acctgtagcg aaaattaata gtgcaataag tggtcctgct gatgatttga ataaagccgt
    11161 cgtaaatgaa cctaaattta aaaattcagg aaagaatgta ccaataatag aaccaagaat
    11221 tagcggtatt accattgcac cgccaggaat tttattcata ccatcatata tcgggacgat
    11281 ggatttagat gtttgagtag acatattaat ctccttagtg tgtcgcatat ttgatatttg
    11341 ccatttttgc acccaaatag agtgcttcag tcatactacg actatctgct gttcctttgc
    11401 ctgctattgg gaaagcagtt ccgtgatcga cagatgttcg aataaatgga agaccgacag
    11461 taacgtttac acctgtatca aagcctaata atttgatagg gatatgccct tgatcgtgat
    11521 acatcacaac aacaatatcg aattcattta ggttagctgc acggtgaaat aaagtatccg
    11581 gagggacagg ccctgtaaca ttaaaccctt tggcttgagc ctcttgaatt gcgggaacaa
    11641 tttctttttg atcttcatcg ccaaacattc cattttcacc tgaatgagga tttaaaccag
    11701 cgactgcaat acgaggttct ttaaacccaa gaatgcgagc attttcttct gctaaatcaa
    11761 taactgtagc aactcgttct ttggtaatta aatcacaggc tttacgaagt tggacgtgag
    11821 tagtaacgtg aataacattg agtttttcac tggttagcat cattgagtaa tctttagtat
    11881 tactgagggt tgcaaggatc tctgtatggc caggaaatgg tttaccacct gcgtgtaggg
    11941 cttctttatt cagtggtgct gtaacaatag cgtggatttt tcctcccatt gctaaattaa
    12001 ctgcctcttc aatatattca taagctgctt ttccagcacg cggatcaact tgagcaaaag
    12061 gcaaatcttc aggcaggtta ttcaaatcta aaatatcaat aacgccatat tcggctacaa
    12121 agttatctag attattgaga attcttaatt tcaattctga atgaacaata ggtaaggctc
    12181 gttgtataat ttttcgatca ccgatcacaa cagggtgtgc taactcataa atccgttgat
    12241 tagctaatgc tttaataata acttctggtc cgataccggc ggcatctccc atcgtaattc
    12301 ccaatatagg tttcatattt actccttgat tgaaattaat cattttgaac gaagtatagc
    12361 ttatataaaa tcataaaagt gtgataaaca tcacaaaagt gatctttttt aatcttattg
    12421 atttaattta atcaatttta aggtggtaaa ttggataggg tatgaaagtg aaaggcattt
    12481 attgctagat aaaagcttcc cttttcaata aaaaagagta tcatacgctc tcttaaacgt
    12541 aaccctacat ttaagtctgt ttccaaccta tcccaatttc tttattcctt aggggcatat
    12601 agggtaaaaa caaaaaagct gaaacgcttt ttttaataag tagcgagtca aatgccccat
    12661 ggcatctatt cttttaggaa tagaaagctt aggctttccg caaggcaccc gacttatttt
    12721 tttcacctct tgtagaaggt ttattttcaa tgacaattaa aactcctatt caagccgttc
    12781 ttgcttcaaa ccaatatttt ttagatcgcc aagatgcaat ggaatcaaac gtgcgtagct
    12841 acccacgtaa attacctttt gcttaccaaa aagcacaagg cgtttgggta actgatgttg
    12901 aaggcaatga atacctcgat ttcctagcag gtgcaggaac gcttgcatta ggtcataacc
    12961 accctaaatt aatccaagca gttaaagatg tattagacag tggtttacct ttacactcgt
    13021 tagatattac gactccgtta aaagatgctt ttacggaaga attactctct ttctttccaa
    13081 aagataaata tatcctccaa tttactggcc cgtctggtgc agatgctaac gaagcggcaa
    13141 ttaaattagc caaaacctac actggtcgtg gcaacgtaat tgctttctcc ggtggtttcc
    13201 acggaatgac ccatggtgcg ttatcgctca ccggtaattt aagtgcgaaa aatgcagtac
    13261 aaggcttaat gccgggcgta cagtttatgc cttatccgca cgaataccgc tgtccgtttg
    13321 gcattggtgg cgaagccggt gcaaaagcag ttgaacatta ttttgagaac tttattgaag
    13381 atgttgaaag tggtgtggta aaaccggcag cggtgatcct tgaagccatt caaggtgaag
    13441 gcggtgtggt tccggctcca atcagcttct tacaaaaagt gcgtgaagtc actcaaaaac
    13501 acggcatctt aatgattgtg gacgaagttc aagctggttt ctgccgttca gggaaaatgt
    13561 ttgcctttga acacgcaggc attgagccgg atatcgtagt aatgtcaaaa gcgattggtg
    13621 gtagcttacc gcttgcggta ttagcgatca aaaaagagtt tgacgcttgg caaccggcag
    13681 gtcataccgg tacattccgt ggtaaccaat tagcaatggc aaccggctat gtatcgctta
    13741 aaattatgtg tgaagagaat ttagcgaaaa acgcacaaga gcgtggcgaa tacttaacta
    13801 acgcattacg tgaattaagc aaagaattac cttgtattgg caatgtgcgt ggtaaaggct
    13861 taatgatggg aattgatatt gttgatgaac gtcagccaaa agatgcaaca ggtgcatacc
    13921 cgcgtgattg cgatttagcc gttgcaattc aaaaatactg ctttaaaaac aaattgttat
    13981 tagagcgtgg cggacgtggc ggtaatgtgg tgcgtgttct ttgtgccgtg aatattaccc
    14041 aagcagaatg tgaagagttt attaaacgct ttaaacaatc tgttgctgaa gcagtgaaag
    14101 cggtgcgtgg ttaatagtta gaatggtagg ggcgaaacat ttttcgccct aatgtaaaaa
    14161 ggggcgaata tttattcgcc cctacaccaa taggatagaa ataatgtcaa acctagaaaa
    14221 acacagacaa tccctgtttt gtagcgatcc aaaatcgatt gcggattatg aatcggcaat
    14281 gaataacgct gtgaaagcgg tttcagactg gctgaaagaa gaaaaaatgt acaccggtgg
    14341 ctcaattaaa gagttgcgtg ccaaaatcaa cggttttaac ccaacccaag ctggtgtggg
    14401 cgtgcaaaaa tcacaggagc atttggtgga aattttctta aagccaagcc tcaaagttca
    14461 ccaccctcat tctttggccc acttacattg cccgacaatg gtaaccagcc aaattgcaga
    14521 agtgttaatt aatgccacca accaatcaat ggactcttgg gatcaaagcc cggcaggttc
    14581 gattatggaa gagcatttaa ttgactggtt acgtcaaaaa gtcggctatg gcaaaggaac
    14641 ttcaggagtc tttacctcag gtggtacgca atcaaacctg atgggcgtat tattggctcg
    14701 tgactgggcg atagcgaacc attggaaaaa ggcagacggt tcagagtggt cggtgcaagc
    14761 cgacggtatt cctgccgatg caatgcaaaa agtgaaagtg gtttgttccg aaaacgctca
    14821 cttctcggtg cagaaaaata tggcaatgat gggaatgggt ttccaatcgg tagtgactgt
    14881 accgtcaaat gccaatgcac aaatggacgt tgaagctctt gcgaaaacgt tggcggattt
    14941 aaaagccgaa ggcaaaattg tggcttgtat cgtcgcaacc gcagggacga ccgatgccgg
    15001 tgcgattgat gatttaaaag cgatccgtaa attagccgat gaacatcagg cttgggttca
    15061 cgttgatgct gcttggggtg gtgcattatt actgtcgaaa gatttccgcc acttcttaga
    15121 cggcattgaa ttaaccgatt ccattaccct tgatttccac aagcatttct tccaatcaat
    15181 ttcgtgtggg gcattcttgc taaaagatga agcgaattac cgctttattg attacaaggc
    15241 agactacctg aattcagaat atgacgaaga acacggcgtg ccgaatttag tgtcaaaatc
    15301 attgcaaact acccgccgtt ttgatgcgtt aaaattatgg tttaccgttg aagccttggg
    15361 tgaggagcta tacgcttcaa tgatcgacca cggggtttat ctttctaaag aagtggaagc
    15421 ctacattaat gccaccgatg gcttggaaat gttagtgccg gcacaatttg cctcagtctt
    15481 attccgtgtc gtaccacaag gttacccggc agaatttgtg gataccttaa accaaaatgt
    15541 ggcagatgag ttatttgctc gtggcgaagc taacattggt gtgaccaaag tcggtgagca
    15601 tcaatcactg aaaatgacta ctttaagccc gattgcaacg cttgaaaacg taagagcatt
    15661 attagctcag gtgttggcag aagcagatcg gattaaagat tcgattgtaa atggaactta
    15721 tgttccgcca attgattaaa ctgcgttaaa aataaaaagg tgttttcgac atttatttgt
    15781 gttttaatgt ttgcattcgg tagtctttat cgaatgcaaa tttttttaac aaggagtttt
    15841 tatgaaaaaa gttctgggtt taacattgat ggcaatggcg ttatcggcgt gtaattcaac
    15901 tgcttccaac ggttcttcac aagcaaatac aggtgttgca ccggcgaatg tgattagcgt
    15961 gaaacaggcg ttagcggcag cagatgacag ctacgtgacg gtagaaggca cactcttaaa
    16021 caaagtcggt gacgaaactt acttatttaa agatgcgaca gggcaaatcc gtgttgagat
    16081 tgacgatgaa gtatgggcag gtcagcacgt tggtagcaaa ggcaataaag tccgtttata
    16141 cggagagatt gacaaaggtt tcaataaaac cgagttagat gttaaaacac tcactgtaat
    16201 caaaccttaa tttggttccc cgccttgttt ggcggggttt ttacgttaat taatcaaacc
    16261 atccttttgc aaaaaaacgc aaaattatac cgcttgttcc ttcaatattg tgctacaaga
    16321 tgctgtgatt tgctgcaaaa tagcggtgta atcagtctct tttaaaccaa tattaccgaa
    16381 atagtgcatt tttacatcac gaatcccaca gaactcaaac agcccttttc ccagcgtatg
    16441 ttcaaatgct gctaaaaagc cttttcgttc atatttttca ttggaattac cgagcgttac
    16501 aaattgttgc atttttttgc cggtcagcaa gccgatactt tcaatttccc cgttacgata
    16561 ggcaaaatca taacttaaca ccctgtctaa atagcctttt aaaattgccg gaaagcccat
    16621 ccaccaaagc ggataaatta acgtaatcac atcagcttca agccaatatt gatgctcaat
    16681 tgccacatct gcttcatagt gtttactgag tgtgccttga aattccgccc agtctaatat
    16741 cggcttgaag ttgagttggt agagatctcg tacaaccacc ttatttggaa cggaagctgc
    16801 tatcgttttt tccaaaatcg catggttaaa gctggaggga ttcgggtgtg cgtaaataat
    16861 caggtgattc atagcgtttt cctttactgt gtcgaggcag ctactagaag attttagcga
    16921 aactttcttt gatgaaaatc aatattttgg cgaatatacc ggcaaatacc ccgtttgagg
    16981 aatgaacggt tttaagtgaa cattagataa tttagcctat tatagtgatg agtaaaaagg
    17041 tgttttcttg actgattatc ctaatcaatc acttcctcgc cgtagctttt tgcgagggca
    17101 atttttagac tcgttaaaaa gccaaaaagt cagtcagcaa gggtatgagc cgattcgtcc
    17161 gccttgggct aatttagcaa attttttaga aaaatgcacc gcttgcggta actgcattca
    17221 ggcttgcgaa acccagatta ttgtaaaagg tgctgcgggc taccctgaaa ttgattttag
    17281 ccgtggtgaa tgcacttttt gtgaaaaatg tgtgcattct tgcgaggcag aggtattccg
    17341 agcagtttca gaacagcctt ggagccataa aattgagatt caagccggct gtttattaaa
    17401 gctcggaacg gaatgccgaa gctgtggcga tagctgtgag atgagagtga ttcgtttccg
    17461 cccgagttta ggcggcattg ccgaaatggt attaaatttg gaaagctgta atggctgtgg
    17521 ggcttgtttg agcgtttgtc cgacaagtgc gataaaaatt cagccattac aaacacaggc
    17581 aaacagagat gagtgaaaaa ttatccgaac aggaaaactg gtatgtgtgc agtctcgtgg
    17641 tgcaagcaaa accggaaaaa ttggaagaag taaaagccga gattctgaag attcctcata
    17701 ccgaaattca cggtgagaaa acagatgaag gcaaattggt cgtgacgatt gaaagcaatg
    17761 tgcatttagc cctttccgat cgaatcgatc aagtaagaga tattaaaggc gtgattgtgg
    17821 tgtctttaat ttctaattac attgatgaac agtgattaat gattattttt gaagccaaac
    17881 gtgggacata gttatggaac tcaatcgtag agattttatg aaagcgaacg ccgctgtggc
    17941 agctgctgca gccgcaggaa ttatgattcc tgtgaaaaac gttcaggcag atgacaccgg
    18001 tattcgttgg gataaagccc cttgtcgtta ctgtggtaca ggttgtagcg tgctagtcgg
    18061 tactaaagac ggtaaagtag tggcaaccca aggtgacccg gatgcagaag taaaccgtgg
    18121 tttaaactgt attaaaggtt atttcttatc caaaatgatg tacggtgcag accgtatgca
    18181 agagccaatg ttgcgtatga aagacggtaa gttcgacaag aacggcgatt tcacgccggt
    18241 ttcttgggat caagccttta gcattatggc ggagaagatt aaaaccacta ttaaaaatga
    18301 cggcccgaat gctgtaggta tgttctcatc aggtcaaaca accatttttg aaggttatgc
    18361 gaaagtgaaa ctttggaaag gtggtttccg ttcaaatacg attgacccga atgcccgcca
    18421 ctgtatggcg tccgctgcgg ttgcctttat gcgtaccttt ggtatggacg agccgatggg
    18481 ctgctataac gacatcgaaa aaaccgatgc gttcgtgctt tggggttcaa atatggcaga
    18541 aatgcacccg attttatggt ctcgtatttc tgatcgccgt ttatcagaca ataaagtgaa
    18601 agtggtggta atgtctactt acgaacaccg ttcgtttgaa ttagccgata caccgattat
    18661 cttcaaacca cactcagatt tagcaatctt aaactacatt gctaactaca ttatccaaaa
    18721 cgataaagta aactgggatt ttgttaacaa acacaccaaa tttaaacgtg gtgaaaccaa
    18781 tatcggttac ggtttacgcc ctgaacacaa attacaacaa aataccaatg cgaaaaccgc
    18841 cggtaaaatg tatgacagcg attttgaaga gtttaagaaa atcgttgccc cttatacatt
    18901 agaagaagta caccgtattt ccggtgtgcc gaaagatcag cttgaaacat tagcaaaatt
    18961 atatgcagat cctaaccaaa aattagtgtc gttctggacg atgggcttta accaacatac
    19021 ccgtggtgtg tgggtaaacc atatgatgta caacgtccat ttattaaccg gtaagattgc
    19081 gttagaaggt tgcggaccat tctcactcac cggccagccg tcagcttgtg gtactgcccg
    19141 tgaagtgggt acatttattc accgtttacc ggcagatatg gtggttacga atccgaaaca
    19201 ccgtgagatt gtagagaaat cgtggaaatt accggctgga gcaattcctg atgtaccggg
    19261 cttccacgct acagcacaaa gccgtgcctt aaaagacggt aaaatgaaat tcttatggca
    19321 gatgtgtacc aacaatatgc agggtggccc gaatatcaat gaagagattt tcccgggctg
    19381 gcgtaaccct gaaaccttta ttgtggtttc agatccgtac ccatctgttt cagcagtggc
    19441 agccgactta attcttccaa cctgcatgtg ggtggaaaaa gaaggggctt acggtaacgc
    19501 agaacgccgt acccaattct ggcgtcagca agtgaaagga ccggcaaatg cccgctcgga
    19561 tacttggcag ttagtggagt tctctaaata ctttaaagta gaagaagtat ggccggaaga
    19621 gttattagcc aaagcacctg aattgcgtgg taaaacacta tatgaaatcc tttatcgcaa
    19681 cggcaaagta gatagctacc aagtgccaac caacattccg ggctatatga atgatgaagc
    19741 cgaacacttc ggttactaca ttcaaaaagg cttatttgaa gagtatgcgg aatttggacg
    19801 tggtcacggt cacgatttag ccgactttga cacctaccac aaagtacgtg gtttacgttg
    19861 gccggtagta aaagacgaga aaaccggcga atacaaagaa actttatggc gttaccgtga
    19921 aggctatgac ccatacgtta aagcgggtga agaagtggca ttctatggca atgcggacaa
    19981 aaaagcagtg attttaggtg tgccatacga gccacctgcg gaagcaccgg acgaagaata
    20041 tgacttatgg ttctgtaccg gtcgagtgtt agagcattgg cacaccggta ctatgacccg
    20101 tcgagtaccg gagttacaca aagcattccc aaccaactta gtttggatgc acccaaatga
    20161 tgcgaaaaaa cgtggcttac gtcatggtga taaagtgaaa gtgatttcac gccgtggtga
    20221 atatgtttct aacttagata cccgtggacg caataaatgt ccggaaggtt tgatttactc
    20281 gactttcttc gatgccggtc agttaatcaa caaagtgaca cttgatgcga ctgatccaat
    20341 ttcatttgaa accgacttca aaaaatgtgc ggttaaagtg gtgaaagctt aattgtaggg
    20401 gcaaattatt tgccccagac aagcggtcaa atttgcaaat ttttttacaa aaacgaccgc
    20461 ttacctaaga ggaacccaca atgaaaatgg atccaaatcg tcgccagttt ttaaaaaatg
    20521 caacccgtac cgcagctggt gtttgcggtg tgggaatcat tttagggcta cagcaacaac
    20581 aaagttttgc ccgccaaggg gtggcgctgc gtccaccggg tgcattgcca gagcaagatt
    20641 tcctgagtgc ttgcacccgt tgcggacaat gtgtgcaggc ttgtccttac gatatgttgc
    20701 atttggcatc gcttatttca ccagtggaag ccggcacgcc atactttatt gcacgggata
    20761 aaccttgcga aatgtgcgtg gatattccct gtatgaatgc ttgcccaacc ggggcattaa
    20821 gcgaagagct aaaagacatt gacgatgcac gaatggggct tgcggtgttg ctcgaccacg
    20881 aaacttgtct taactggcaa ggcttgcgtt gtgacgtgtg ttaccgtgtt tgtccgttaa
    20941 tcgacaaagc gattactctt gagaaagtgc ataacgaccg tacctcgatt cacgccaaac
    21001 tgattccgac cgtgcattct gatgcttgca ccggttgcgg taaatgtgaa caggcttgcg
    21061 tattggaaga ggcagcgatc aaagtgttac cgatggattt agccaaaggc ttgctcggtc
    21121 gtcactatcg tttaggctgg caagagaaac aaaatgccgg taaatcgctg attgaagatg
    21181 ttcacccaga gggtttacgc ccagcttatg atgccagaat gccggaaggc ttacacgagc
    21241 cggtgtatca gcatatggaa gtaaaacctg atgtgaaagt ggctacgcca agtcgggcga
    21301 cacaaaacta tgtgccgaac tctacgacgg tggaagctcc ggagcatttc cctgatttag
    21361 acttgaatct caagggggtg aaataatggc agccaattct ccaaaaaatg cgggcttaga
    21421 ggctcgtcag aaactaggtt ggtggcacgc ttaccgcttt ttgattttaa gacgcttaag
    21481 ccaactcagc attattttga tgtttttaag cggcccactt tggaatgtat ggattttaaa
    21541 aggaaactac agcggtagct tattactaga taccgtgccg atgacagacc cattaatgac
    21601 cgctgaagtg ctggcaaccg gctatcgccc tgaatggacg gtattggttg gggcgttaat
    21661 tatggtggcg ttttacggca ttgtcggcag caagctgttt tgtagttggg tttgcccgat
    21721 gaatatggta acagatactg ctgcttggtt aagacgcaaa ctcggtattc gccaaactgc
    21781 taaaattcca cgcaatttac gctatgtgat tttggcagtc attttagtcg gtagtgcaat
    21841 ttccggcacg ctattgtggg agtggatcaa ccctgttgcg gctctcggtc gggtgtttgt
    21901 attcggctta ggggcaatgc tctggctggt tttagtggtg ttcttgtttg acttattggt
    21961 agcagagcac ggctggtgtg gtcatctttg cccgattggt gcaacttatg ccttaattgg
    22021 ggcgaaaagc ctgattaaag tcaaagtcgt ggatcgtaac cgttgtgata attgtatgga
    22081 ttgttacaac gtttgcccgg aaccccaagt gttgcgtgtg ccgctacacg gtaaaccgga
    22141 ggaaagcacg attatcttat ctaaagattg cattacctgc ggacgttgta ttgatgtttg
    22201 tgccgaaaat gtgtttgcct tcggaagtcg ttttcaaggc agtattgatg tgaaaaatat
    22261 ttaattttta tctttttgaa aacaagaatt ttaatggagt gaacctcaaa tgagaaaata
    22321 tctcgcctta attttagctg tgatgtccgg ctttgcaatg gcggaagccc cgaagttatc
    22381 cggcactatt gatgagacaa ccgaaagtgt tgcaccggga tttcataacc cacaaaaaga
    22441 tgtgggcacg attccaacca ctttcccttt ccaaccacca cttgttccac atagtattcg
    22501 tggcttacaa gtgagcaaaa acgtaaacca ttgtttaggt tgccacgcta ttgaaccatc
    22561 taaaaccaca ggtgcgaccc gtttaccgcc aagccatttt accgaccgtg atggcaaggt
    22621 gtacgaaaac gaatctcctc gtcgttattt ctgtttgcaa tgccacgtgc aacaaactga
    22681 tgtaaacccg attattcaaa ataaatttga atctgtacgt gaatctcaag ctaaataagg
    22741 agtaagtaat gttgagaaga ttttgtaact ggttacgtag cccaagcaaa atggcaattg
    22801 gtgctgtaat ccttatttct gcattaggtg gtattttcgc ttggagtggt tttaatgcag
    22861 ggttagcaat gacaaatact gaagagtttt gttcagattg ccatatgaac gatgttgtgc
    22921 cggaataccg tgcctctgct cactattcaa accgcagcgg tgtgaaagcg atttgttcag
    22981 attgccacgt accgcacgaa ttctttccaa aatggcaacg taaaatcata gccgcaaaag
    23041 aagtttatgc acactttatc ggtaaagtag ataccaaaga gaaatttgaa gctcatcgtg
    23101 gcgaaatggc agaacgtgaa tgggcacgta tgaaagcgaa cggctctcaa gagtgccgta
    23161 actgtcataa ttttgatgat atggacttca ctcaacaaaa aaacattgcg cagcaaatgc
    23221 acgcattggc acaagagcaa ggtaaaacct gtattgactg ccacaaaggg attgcacaca
    23281 atctcccaca tatggaagcg atccagaaaa actttatccc tgatgatttg ctgaaaaaag
    23341 cagaagagaa gaaataactt aacgtgtttc aatagaaaac cctcaatttc cattgagggt
    23401 ttctttttag tagccaagtt agtaaccaat ggcattgaca tttggtaaaa aatcgttgga
    23461 atttaaccgc ttgacgaccg tttctatcgt gccgttttcg tgcaattcaa tttcacgcca
    23521 gccttgtggt tgtaagtcta aggtaaactg gtcgcaattc ggcttaaatt gaatgcaagt
    23581 agatggggtg gcataaatcg gaataccatg ccaaatatca tcaacctgct gatgaatatg
    23641 accatgcaca atcgctttta cattcttaaa ttttgcgaaa atctcggata attcattgct
    23701 gttttttaag ctatgctgat ccagccacgc tgagtttgtc ggcaaaatat tgtggtgcag
    23761 agcaaccaag gtaaaccgtt gcggatattg tgccaatttt tcttctaacc atttaagctg
    23821 tgagtaagag agttcgccat aaggcatacc taccacctgg gtatttagca tcagaagctg
    23881 ccattgctca ccgagtaaaa tttgtttatg gggaaggata tgtttatatt gcgccaaaat
    23941 ttctgacata ctcggctgaa catcgtgatt cccctctagc cacactatcg gcgagtttag
    24001 cgaattagtt atctgtgcaa aatagtgata accagcaata ttgtgatctt gaatcaaatc
    24061 acctgtggca agaactagat caaaatattg cttcgcttgt ttgtttattt gctcaataac
    24121 tgccgaaaaa ctttttactg ttttgacatt aagtagagtt tcctccggat tggagaaaag
    24181 gtgagggtca gttatttgta ataacttaat tacccctgtt acgggtaact taatatattc
    24241 gtgcattcgt tttgcatcca ttcaaattct agtaattttt aactggtgag attctatcag
    24301 taagctaaaa gtaaaaatgt ggactatgcc gcatttttga aaaaaagtga gattaatcaa
    24361 aaaaatttat tattttcgat agctaataca atgataagta ttgattaata ttttcatatt
    24421 gtaatagatg atttttatac tgtttggctg tttctagaac aacttctcta tattt
//
LOCUS       DANKBV010000023        24405 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.023, whole genome shotgun sequence.
ACCESSION   DANKBV010000023 DANKBV010000000
VERSION     DANKBV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24405
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.023"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(130..1533)
                     /locus_tag="PWY43_000725"
     CDS             complement(130..1533)
                     /locus_tag="PWY43_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2135625.1"
                     /translation="MLTYTFNTQEGTALYEQLYRFLKNDIESNILIAGDKLPSKRAFA
                     KHLGISVMTVETAYQQLVAEGYVNTQPKKGFFVNALNLPEKKEAISVQLQKISSEQYE
                     KNKKWQADLTNRQTLAENFPFSVWTKLVREVLKTHQSELMERAESGGVWTLKFAIANH
                     LRAFRGMIVNPTQIIIGAGTEYLYGLLIQLLGLDKHYALTEPSYDKLRRIYHSYRLST
                     ATVSMPFSIDELNSKNVDVIHTSPSHHFPTGHVMPIAKRYELLAWVAQKAERYIIEDD
                     YDSEFRFVGQPIPALKSIDMLGRVIYMNTFSKTLSSTVRIAYMVLPPELLNRFQQELG
                     FYASTVSNFEQYILAEFIQQGYFEKHINRMRAYYQKKRDKLLKAFKQGNLAEKIAIKE
                     EHSGLHFIVQLNTTLTERQILQAAEEQGIKFVALSAYYQDKSQIQQNAFIIGYSNLKD
                     DQINYVAESLKKILDNE"
     gene            1744..2616
                     /gene="pdxS"
                     /locus_tag="PWY43_000726"
     CDS             1744..2616
                     /gene="pdxS"
                     /locus_tag="PWY43_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDL2135626.1"
                     /translation="MTNRYDLNRQLAQMLKGGVIMDVTTPEQACIAEEAGACAVMALE
                     RIPADIRAAGGVSRMSDPKMIKSIQAAVSIPVMAKVRIGHVTEAQILQAIEIDYIDES
                     EVLSPADDTFHINKRKFEVPFVCGAKNLGEALRRINEGAAMIRSKGEPGTGNVVQAVH
                     HLRKMKQEIARLVSHTTDELYHVAKELQVPFDLVQYVHQYQKLPVVTFAAGGIATPAD
                     AALMMQLGAEGVFVGSGIFKSGDPAKRARAIVQAVTNYNDPKLLAELSENLGEAMVGI
                     NEQEIELLMAARGV"
     gene            2616..3188
                     /gene="pdxT"
                     /locus_tag="PWY43_000727"
     CDS             2616..3188
                     /gene="pdxT"
                     /locus_tag="PWY43_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDL2135627.1"
                     /translation="MKIAILALQGAFSEHTDKLQQLGVESMEIRQLADLNQDFDGLIL
                     PGGESTVQGKLLRELGLLEPLRQKILNGLPTLGTCAGLILLAENLVNDDKKHLATLPV
                     TVKRNAYGRQLGSFLVEEEVKYLGKIPLTFIRAPYIESTGKDVEILAKISGKIVGVKY
                     QNQIGISFHPEVNNDLSFHRYFVEMCRKNK"
     gene            3337..4287
                     /gene="tal"
                     /locus_tag="PWY43_000728"
     CDS             3337..4287
                     /gene="tal"
                     /locus_tag="PWY43_000728"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDL2135628.1"
                     /translation="MSQLDTLSKMTVIVADTGDIEAIKLYKPKDATTNPSLILSASAL
                     PQYAPLIEEAINYAKAQSSDKDQQLVDAEDKLAVNIGLEILKLVPGRISTEVDARFSY
                     DTEKTIAKARKLIGLYNDAGISNDRILIKIASTWQGIKAAEQLEKEGINCNLTLLFSQ
                     AQARACAEAGVYLISPFVGRILDWYKAHEGKEYAPAEDPGVISVTNIYNYYKQHGYDT
                     IVMGASFRNVGEIIELAGCDRLTISPPLLKELQESDEKLVCKLDYKGEIKPRPEKMTE
                     AEFYWEHNQEPMAVEKLAEGIRKFAIDQEKLEAMLAEKLQ"
     gene            complement(4349..4624)
                     /locus_tag="PWY43_000729"
     CDS             complement(4349..4624)
                     /locus_tag="PWY43_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2135629.1"
                     /translation="MKKLSLAVLASLMLAACTADVYSDKGNATVLSSKAVSNDVVELT
                     VQRDNGETVTLTREYDAHAAVGARVHLADEIKNEDSDLKTIRRYEFK"
     gene            complement(4655..5119)
                     /locus_tag="PWY43_000730"
     CDS             complement(4655..5119)
                     /locus_tag="PWY43_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL2135630.1"
                     /translation="MKKLALAVALMSSFALVGCANTDVFSGSVYSGEQAKEARSIAYG
                     TIVSVRDVKIQARSEGVIGTVGGGVLGGVAGNAIGGGTGQAIATAVGAVAGAVIGNQV
                     EQKASQVSSLEMVIRKDDGKEIVVVQKKEDGFVPGKRVRIVGSNSDLNVSLL"
     gene            complement(5140..5739)
                     /locus_tag="PWY43_000731"
     CDS             complement(5140..5739)
                     /locus_tag="PWY43_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2135631.1"
                     /translation="MDKIIAENAKFFEKMTACFGDLSQFEQKIRQIAEIAGIDLAAYE
                     IDHLAVRMNQTETAEQWKILLLDGATLLKESEVNGRPIGLFALIQAVEFCGQFVKIVE
                     LPFPKNKVYLEEGWEHIELVFPMLDNESSEQWVSRALSHFRLADDSEIKLKISQPQVE
                     GERLPNPSIAISLRNVTFCNPYCLKLHPYDIKSVIMSEN"
     gene            5773..7500
                     /gene="argS"
                     /locus_tag="PWY43_000732"
     CDS             5773..7500
                     /gene="argS"
                     /locus_tag="PWY43_000732"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL2135632.1"
                     /translation="MNIQQILSDKIKQAMVASGADSNVEPLVRQSGKPEFGDYQANGA
                     MGAAKKLGMNPREFAQKILENADLNGIADKLEIAGPGFINIFLNPEWLAENTNSSLQA
                     VNFGIKTANPQTIVIDYSSPNVAKEMHVGHLRSTIIGDAVVRTLAFLGNNVIRANHVG
                     DWGTQFGMLIAYLEKMENEHVSEMELSDLEAFYRAAKEHYDSDEAFAEKARNYVVKLQ
                     SSDKYCLTMWKKLVDITMHHNQENYNRLNVTLTEKDVMGESLYNPMLPEIVADLKAKG
                     LAVEDDGALVVFLDEFKNKDGDPMGVIVQKKDGGFLYTTTDIAAAKYRYETLKADRAL
                     VFSDSRQAQHMQQAWLITRKAGYVPDSFSLEHPFFGMMLGKDGKPFKTRSGGTVKLKD
                     LLDEAVERADKLIAERSTELTAEEKAAVVEAVAIGSVKYSDLSKNRTTDYVFDWDNML
                     TFEGNTAPYMQYAYTRIRSIFARANIDEDALSGSIQLNEPKERALAVKLLQFEEALNG
                     VAKEGMPHILCQYLYELAGSFSSFYEACPILNAEETTKNSRLKLAALTAKTLKQGLDL
                     LGIKTVEKM"
     gene            7690..8856
                     /gene="cgtA"
                     /locus_tag="PWY43_000733"
     CDS             7690..8856
                     /gene="cgtA"
                     /locus_tag="PWY43_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDL2135633.1"
                     /translation="MKFIDEALIRVEAGDGGNGCVSFRREKFIPKGGPDGGDGGDGGD
                     VYLIADENLNTLIDYRFEKRYAAGRGENGRSAGCTGHRGQDITLRVPVGTRAIDNDTQ
                     EIIGDLTRHGMKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLKLELLLLA
                     DVGMLGLPNAGKSTFIRSVSAAKPKVADYPFTTLVPSLGVARVGADRSFVVADIPGLI
                     EGAADGAGLGIRFLKHLERCRILIHLVDIMPIDESDPAHNISVIESELYQYSEKLAEK
                     PIWLVFNKIDTIGEEEAAERAKDIAEQIGWEGDYYLISAATGQNVQALTRDIMDFIEA
                     NPREVEEENKAPEEVKFKWEDYHNEAMQNAVEEDWDDDWSEEDDEGIEFLYERR"
     gene            8941..9558
                     /locus_tag="PWY43_000734"
     CDS             8941..9558
                     /locus_tag="PWY43_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL2135634.1"
                     /translation="MLSSTSCDLFNIPFFQFAQMKKYCPEEIPRIKAEYKTQWEIWKK
                     LHQEVSRQLGQPFAPPHIEKWCNGWQVRAHFFAYYKYEFNRNSAAILSVILNRRRLQV
                     SLDWHCYRADRSQINVHQYNQWWENLDREKFADFEIWHGSESEYDDFRTVSQMSEQAF
                     TLTNEEDFWCIGKNVEKDDLDNIDVVEFIYQTIRALLPLYEKCHQ"
     gene            complement(9605..11563)
                     /locus_tag="PWY43_000735"
     CDS             complement(9605..11563)
                     /locus_tag="PWY43_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDL2135635.1"
                     /translation="MIAELGNYTLALALAISILLAVLPLWGAEKQNSTLIALARPMTW
                     AMFLALTVSFGSLFYLFAVNDFTVQYVVNNSNSRLPLQYRLSAVWGAHEGSLLLWVWL
                     LSLWSVGVATFTRKMPEDAVARVLGLMGVIGIGFLMFVIFTSNPFDRTFPNFPIDGKE
                     LNPMLQDVGLIFHPPLLYMGYVGFSVAFAFAIASLMTGRLDTAWARWSRPWTMAAWVF
                     LTLGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLAGTALIHSLAVTEKRGTFKAWT
                     VLLAILAFSLCLVGTFLVRSGILVSVHAFASDPTRGLYILAYLVLVVGGSLLLYAFKG
                     SEIKSLDNYERYSRETMLLINNILLMAFLSVVFLGTLLPLIHKQIGLGTISIGAPFFD
                     QMFLILMVPFSFVLGIGPLVKWRRDQVSAIRKPVIIATILMALLGFGLPYLFRNTITV
                     SVVLGTMMSVFIVLLSLYELHQRATHRHSFLSGIFKLSRSHWGMVLAHLGVAMTVFGI
                     AFSQNFSIERDVRMNVGDKAEILDYQFEFKGIKITDGANYQGGTAELEITRNGKYEAT
                     LNAEKRFYTVSRMGMTEAAIDWGFTRDLYAALGEKLEDNSWAVRLYYKPFIRWIWIGG
                     LFMALGGLLCMLDKRYRLRIENKKEVIA"
     gene            complement(11560..12117)
                     /gene="ccmE"
                     /locus_tag="PWY43_000736"
     CDS             complement(11560..12117)
                     /gene="ccmE"
                     /locus_tag="PWY43_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDL2135636.1"
                     /translation="MNPRRKSRLKVVVSILLGLSVAAGLTLYALSQNIDLFYTPSEIV
                     NGKNDDPKQKPEVGQRIRVGGMVVEDTVKRDDKSLKVVFDLNDIGPSITVEYEGILPD
                     LFREGQGIVAQGVLIELTRLKATEVLAKHDENYMPPELGDKLKEQHKKSGVSEQDLKG
                     ESETDRKMKAEAEAEKANNVQGESK"
     gene            complement(12303..12500)
                     /gene="ccmD"
                     /locus_tag="PWY43_000737"
     CDS             complement(12303..12500)
                     /gene="ccmD"
                     /locus_tag="PWY43_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDL2135637.1"
                     /translation="MTFQFESISDFFAMGGYGFYVWLSYGVSFIAMIGLIWLSRREQK
                     AILAQAKKEIVREEKLKQTSQ"
     gene            complement(12515..13252)
                     /locus_tag="PWY43_000738"
     CDS             complement(12515..13252)
                     /locus_tag="PWY43_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HDL2135638.1"
                     /translation="MWKWLHPYAKSETQYHLLGKIQPFLGWLSLIMLAAAFVWGLAFA
                     PKDYQQGDSYRIIFIHVPAAIWSMGVYGSMAVAGLVALVWQIRQANLAMISMAPIGLV
                     FAFISLATGAIWGKPMWGTWWVWDARLTSALVLFFLYIGVMALYSAFQDRQTGAKAAA
                     VLSVVGVINLPIIHFSVEWWNTLHQGATITKFDKPSMAVEMLIPLLLAIFGSLIFTAW
                     FSIWRYRIALLKDERNRPWVKQLAKSV"
     gene            complement(13452..14114)
                     /gene="ccmB"
                     /locus_tag="PWY43_000739"
     CDS             complement(13452..14114)
                     /gene="ccmB"
                     /locus_tag="PWY43_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDL2135639.1"
                     /translation="MILLTIIQRELTIAFRKPSEILNPLWFFLMVITVFPILMGPNPE
                     LLGKIAGGIAWVAALLSALLSFERLFRDDYLDGSLEQLMLLPLALPQVALAKVIAHWL
                     LTGLPLILLSPIAAILLSLETHVWWALVLTLLLGTPILSCLGAIGVALTVGLRKGGTL
                     LSLLILPLFLPVLIFAAAVLEAATLNMGYSGQLAIIGAILAVTLTFSPFAIAGALRIS
                     LN"
     gene            complement(14111..14752)
                     /gene="ccmA"
                     /locus_tag="PWY43_000740"
     CDS             complement(14111..14752)
                     /gene="ccmA"
                     /locus_tag="PWY43_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDL2135640.1"
                     /translation="MNQTAQYQLKLNQIACERGETRLFEGCSFTVQSGEWVQIEGHNG
                     IGKTSLLRILAGLSLPAEGEVLWNDIAIQKQRDHYYSELFYLGHYSGVKPELTPWENL
                     RFYQKIQQLPQDDDALWHALTKVSLIGREDLPCSHLSAGQQRRVALAKLWLTNAKLWI
                     LDEPFTAIDKKGVADLIAHIEQHCEQGRLVIFTSHQAAESDKVRIISLDQFKV"
     gene            complement(14896..15606)
                     /gene="hda"
                     /locus_tag="PWY43_000741"
     CDS             complement(14896..15606)
                     /gene="hda"
                     /locus_tag="PWY43_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HDL2135641.1"
                     /translation="MQLPLPIHQIDEDTFDNFYSQNSEVLLESLKRNFTDVQQPFFYI
                     WGGKSCGKSHLLKAVSNHFLLNNQTSSYIPLTKANYFSPLVLDNAELLNVICLDDIQA
                     IAGDAEWELAIFNLFNQIREQQGLFNEGQKTLLLISADCPPHQLNIQLPDLRSRLTWG
                     EVYQLNDLTDEQKCQILQLNAHQKGVELSDEVAHFLLKKAGTDLEQLSKILDKLDKAS
                     LQAQRKLTIPFVKEILSL"
     gene            15876..17477
                     /locus_tag="PWY43_000742"
     CDS             15876..17477
                     /locus_tag="PWY43_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2135642.1"
                     /translation="MKNMTKLSLITLALAASSSVFAAPKTFVYCLEASPSALSPVLST
                     DGASFDVNALPIYNKLTDFEDGTTNVIPSLAEKWDISEDGKTYTFHLRKGVKFHDNKE
                     FKPSRELNADDVVFSINRQFDPNHPFHKVSGGNYEYFIGMDMQNIIDKVEKVDDHTVK
                     ISLKVPNAPFLANLAMDFNAVYSAEYAEQSLKAGKPERIDTNPIGSGPFQFVDYQKDA
                     TVRYKAFENYWQGKAKIDRLVFAITPDASVRLAKLQKGECHAMPYPNPADIENLKKDP
                     NIELMSQSGMNIGYLALNSSIKPLDNQKVRQALNHAVNKQAIVDAVYQGAGQVAKNPI
                     PPTMWSYNEAVQDYDYNPEKAKALLKEAGFENGFDTELWAMPVSRPYNPNARRMAELI
                     QEDWKKVGVNAKIVSYEWGEYLKRLRQGEAATSMIGWTGDNGDPDNFLNTLLSCSAVE
                     AGSNYAKFCHKPFEEVVLGAAQESDKAKRTELYKQAQVIFKEQAPWITIAHSTVYFPV
                     RKEVKGYVMSPFSLHNFYKVDLADK"
     gene            17749..18753
                     /locus_tag="PWY43_000743"
     CDS             17749..18753
                     /locus_tag="PWY43_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2135643.1"
                     /translation="MFSFILKRVLMVIPTFIAITLITFALVHFIPGDPIEIRMGERGL
                     SPEVHQQMMEQLGLNRPLFEQYISYIKGVLHGDLGNSFRNNEPVLKEFFTLFPATVEL
                     AFFALLWSLIFGIILGVIAAVKKESWISHTVTTLSLTGYSMPIFWWGLILILYFSTPL
                     GLPASGRLPSEYWIEAETGFMLIDTWNSDEPGAFLAAIKSLILPSIVLGTIPLAVVTR
                     MTRSSMLEVLGEDYIRTAKAKGLNTTRIVIVHALRNALIPVVTVVGLIVGQLLSGAVL
                     TENIFSWPGIGKWIIDAINSRDYPVLQGSVLIIATIIILVNLTVDLLYGIVNPRIRHS
                     "
     gene            18855..19748
                     /locus_tag="PWY43_000744"
     CDS             18855..19748
                     /locus_tag="PWY43_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2135644.1"
                     /translation="MSGITAVNPPAPKTPLQEFWYYFCQNRGALIGLTFIAIVFVACV
                     FAPWVAPFDPIEQNRSALLLPPAWLEGGNSAHLLGTDDIGRDILSRIIYGSRLSVFIG
                     LVIVLLSCVFGVILGLLAGYYGGTLDIIIMRFVDIMLAIPSLLLTIGVVTILGPSLIN
                     AAIAIAIVSIPSYVRLTRASVLSEKNRDYVTASRVAGASVLRLMFVVILPNCLAPLIV
                     QMTMGISNAILELAALGFLGIGAQPPTPELGTMLAESRGFMQSANWLVTIPGLAILSL
                     VLAFNLMGDGLRDALDPKLKQ"
     gene            19759..20748
                     /gene="dppD"
                     /locus_tag="PWY43_000745"
     CDS             19759..20748
                     /gene="dppD"
                     /locus_tag="PWY43_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDL2135645.1"
                     /translation="MALLTVDQLSVHFGETKTPFKAVDRISYEVNEGEVLGIVGESGS
                     GKSVSSLAIMGLIDFPGRVMAEKLYFNGNDLLALKPKEKQQIVGADVSMIFQDAMTSL
                     NPSYTVGYQIMEALKVHQGGSKASRRERAIELLTMVGIPDPESRLEVYPHQLSGGMSQ
                     RVMIAIAIACRPKLLIADEPTTALDVTIQAQIIDLLLELQRKENMALILITHDLALVA
                     ESAHRIIVMYAGQVVEEGKAEQIFKAPLHPYTQALLKALPEFAEGKSRLQSLPGVVPG
                     KYDRPQGCLLNPRCPYATEKCRTIEPAMQMINGRQVKCHTPLNAQGEPIRIAV"
     gene            20876..21865
                     /locus_tag="PWY43_000746"
     CDS             20876..21865
                     /locus_tag="PWY43_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2135646.1"
                     /translation="MKENNSLPPLLKAVDLKKYYPVKKGMFAKPKTVKALDGVSFTLE
                     RGKTLAIVGESGCGKSTLGRMLTMIESPTEGELFYNGQNFLVNDKETAQLRRKKIQIV
                     FQNPYSSLNPRKKVGSILEEPLLINTNLSAKERKEKVLEMMAKVGLKPEFYDRYPHMF
                     SGGQRQRIAIARGLMLQPDIVVADEPVSALDVSVRAQVLNLMMDLQEEMGLSYVFISH
                     DLSVVEHIAAEVMVMYLGRCVEQGDTKTIFSNPRHPYTQALLSATPRLNPEQRRERIK
                     LTGELPSPLNPPKGCAFNARCRFATDRCYVERPELKTYQDGTRMACFVVEENN"
     gene            complement(22103..22675)
                     /locus_tag="PWY43_000747"
                     /pseudo
     CDS             complement(22103..22675)
                     /locus_tag="PWY43_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            22845..23888
                     /gene="msrB"
                     /locus_tag="PWY43_000748"
     CDS             22845..23888
                     /gene="msrB"
                     /locus_tag="PWY43_000748"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2135647.1"
                     /translation="MKYNQLLLCLVSAIFTTSSIANITTPNEMRSENLQDIYLAGGCF
                     WGIEAYMEKIYGVKEATSGYANGKTEQTAYRIIGQTDHAETVHVTYDANKISLEKLLQ
                     YYFKVIDPTSKNKQGNDIGRQYRTGIYYQNEQDKQIILKEIQAQQAKYKQKIQVQVEP
                     LKHYILAEEYHQDYLKKNPNGYCHIDLNQANSIIIDPKDYPKPSDATLKMKLTPLQYS
                     VTQRKNTERSFSNEYWDNTAPGIYVDITTGEPLFSSLDKYDSGCGWPSFTKPIINEVV
                     NYTEDNSFNMTRTEVLSRSGNAHLGHVFTDGPKDKGGLRYCINSASIKFIPLEQMEKE
                     GYGYLINMIKGNK"
     gene            23891..24373
                     /locus_tag="PWY43_000749"
     CDS             23891..24373
                     /locus_tag="PWY43_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL2135648.1"
                     /translation="MKKIISLLIMMLSLNSYAQENSHLGHIPLKDLNQNQITLDKYQG
                     KKIYIKAWASWCSICLAGLAEIEEISQKANNDIEIITIVSPGHKGEKTTNQFIEWYKG
                     IESDYPNITVLLDEQGDFIKQTEIRGYPHNLLLDSSLNLKKSIPGHLNSEQILHSITN
                     "
BASE COUNT         7543 a         5112 c         4940 g         6810 t
ORIGIN      
        1 atgcattata cgcaaaacaa aatgccttgc aaggagaatt tggcaatttt ttgcaaaaat
       61 cactaaaata attaccactt gcgtgttttt aaggcaattt gctaaaaatt aaagcagttt
      121 tgatacttgc tattcgttgt ctaaaatttt tttcaggctc tctgctacat aattaatctg
      181 atcatctttt aaattagagt acccaattat aaaagcattt tgctgaattt gagatttatc
      241 ttgataataa gcagacaatg cgacaaattt tataccttgt tcttctgctg cctgcaaaat
      301 ttgcctttct gttaatgttg tatttagctg tacaatgaaa tgcaagcctg aatgttcttc
      361 ttttattgca atcttttctg ctaaattgcc ttgcttgaaa gcttttagta atttatccct
      421 ctttttttga taatacgctc gcattcgatt gatatgcttt tcaaaatagc cttgttgaat
      481 aaattctgcc aaaatatatt gctcaaaatt ggaaacggta gaagcataaa accctaattc
      541 ctgctgaaaa cgattcagta attctggtgg caaaaccata taggcaattc gtacagttga
      601 agagagcgtt ttagaaaaag tattcatata gataactcgc ccgagcatat caatagattt
      661 caatgcagga attggttgtc ctacaaaacg aaattcgctg tcgtagtcgt cttctataat
      721 atatctttca gctttttgtg caacccaagc taacaactca taacgtttgg caatcggcat
      781 tacgtgtcct gtcggaaaat ggtgtgatgg cgaggtatgt atcacgtcca cattctttga
      841 atttaactca tcgattgaaa atggcatcga tactgtggct gttgataagc gataactgtg
      901 ataaattctg cgcaatttat cgtaactggg ctcagttagt gcataatgtt tatctaagcc
      961 tagaagctga atgagcaaac cgtaaagata ctccgtaccc gctccaataa taatctgtgt
     1021 tggattaacg atcattcctc taaaggctcg aagatgattg gctatcgcaa attttaatgt
     1081 ccatacaccc ccactctcag cacgctccat tagctcactt tggtgtgttt tcagcacttc
     1141 gcgtactaat tttgtccaaa ccgaaaacgg aaaattttct gctaaagtct gtcgattagt
     1201 taaatctgcc tgccattttt tatttttctc atactgttct gaactgattt tttgtagctg
     1261 aactgatatg gcttcctttt tttctggtaa atttaaagca ttcacaaaaa agcctttttt
     1321 aggctgagta ttcacataac cttctgctac taactgttgg taagctgttt ccactgtcat
     1381 tactgaaatg cctaaatgct ttgcaaaagc ccgtttagac ggcagtttat cccctgctat
     1441 aagtatgttg ctttcaatgt cgttttttag aaaacggtaa agttgctcat agagagcggt
     1501 gccttcttgt gtgttaaaag tgtaggtaag cataatctga catgataaga aaaacataaa
     1561 atggtcattt taaacagttc agattaaatt acaatacatg ctgtttcctc actcaaattg
     1621 gctgagtgta tttaaattgg tagatgaaca ctcgcatcca gcgaatataa aaataaactt
     1681 aatgccgagc taatcgaaac tacgaaaagt tcaattattc aacaattaga aggaaaaagt
     1741 acaatgacta atcgctatga tttaaacaga caacttgctc aaatgttaaa aggcggtgta
     1801 attatggatg taactacccc tgagcaagcc tgcattgcgg aagaagctgg agcttgtgca
     1861 gtaatggcac tagagagaat tcctgcagat attcgtgcag ctggtggcgt ttctcgtatg
     1921 agtgatccca agatgataaa gagtatccaa gcagcggtat ccattcctgt aatggcgaaa
     1981 gtacgtattg gacacgttac agaagcacaa atcctgcaag cgatcgaaat tgactatatt
     2041 gacgaaagtg aggtgctttc tcctgcagat gataccttcc acattaacaa gcgtaaattt
     2101 gaagtgccat ttgtatgtgg agcaaaaaat ttaggcgaag ccctacgtcg tattaatgaa
     2161 ggcgctgcta tgatccgtag taagggcgag ccaggaacag gcaatgtggt gcaagcagta
     2221 catcatcttc gcaaaatgaa acaagaaatt gcccgcttgg tttcacatac cacagatgaa
     2281 ctttatcacg tagctaaaga gctccaagta ccatttgatt tagtacaata tgtacatcag
     2341 taccaaaaat tgcctgttgt cacctttgct gctggaggta ttgcaacccc tgccgatgct
     2401 gcattgatga tgcagttagg tgcagaagga gtcttcgttg gctcaggtat tttcaagtca
     2461 ggcgatcctg ctaaacgagc gcgggccatt gtacaagctg ttaccaacta caacgatcct
     2521 aaattacttg cggagctttc tgaaaattta ggtgaagcaa tggtaggaat taatgagcaa
     2581 gagattgaat tgctaatggc agcaagaggt gtctaatgaa aattgctatt cttgctctgc
     2641 aaggtgcatt tagcgagcat actgataaac tccagcaact aggggttgaa agtatggaaa
     2701 ttcgtcaact tgctgattta aaccaagatt ttgatggtct gattttacct ggtggcgaat
     2761 cgactgttca aggtaaatta ttacgtgaat taggcttgct tgagccgctt cgtcaaaaaa
     2821 ttttaaatgg cttgccaacg cttggaactt gtgcgggttt aattttgctt gcggaaaatt
     2881 tggtaaacga cgataaaaaa catttggcta ctttgcctgt aacagtgaaa cgtaatgctt
     2941 acggaaggca attaggctcg tttttagtag aagaagaagt taaatatctc ggtaaaattc
     3001 ctctcacatt cattcgtgcg ccttatatag aaagtactgg aaaagatgta gaaattttag
     3061 ccaagataag cggtaagatt gttggagtaa aataccaaaa tcagataggt atatccttcc
     3121 atcccgaagt gaataatgat ttaagttttc accgctattt tgtggaaatg tgccgaaaaa
     3181 ataagtaaaa gaaaaggggc tattgcctct tattttttat acttttttag aaaaagtagt
     3241 tttgaataaa aaaatactaa aactctattt cccttatgtt attattatag taacttttac
     3301 tactatttat ttcacaatca aaaaaggaat cagactatga gccaacttga caccttaagc
     3361 aaaatgaccg ttatcgttgc tgacactggt gatattgaag caatcaaact ttataagcca
     3421 aaagatgcaa caacaaaccc gtctttaatt ttaagtgcat cggcattacc gcaatatgct
     3481 ccattaattg aagaagcgat taattacgca aaagcacaaa gtagcgataa agatcaacag
     3541 ttagttgatg ctgaagataa attagcggta aatattggtt tagaaatctt aaaacttgta
     3601 cctggacgta tttctacaga agttgatgct cgtttttctt atgatactga aaaaactatc
     3661 gctaaagcac gtaaattaat tggattatat aacgatgcag gcatcagtaa tgatcgcatt
     3721 ttaattaaaa ttgcctctac ttggcaaggt ataaaggcag cagaacagtt agaaaaagaa
     3781 ggcattaact gtaacttaac gttattattc tctcaagctc aagctcgtgc gtgtgcagaa
     3841 gcaggggttt acttaatctc tccattcgtt ggtcgtattt tagactggta caaagctcac
     3901 gaaggtaaag aatatgctcc agctgaagat ccaggtgtaa tttcagtaac caatatatac
     3961 aattactaca aacaacacgg ttatgacact attgtaatgg gtgcaagttt ccgtaatgta
     4021 ggagagatca ttgagttagc tggttgcgac cgtttaacca tatcaccacc gttattaaaa
     4081 gagctacaag agagcgatga aaaattagtt tgcaagctag attacaaagg tgaaatcaag
     4141 cctcgtccag aaaaaatgac agaagcagaa ttctactggg agcataacca agagccaatg
     4201 gcagtagaaa aattagctga aggcattcgt aaatttgcga ttgaccaaga gaagttagaa
     4261 gcaatgcttg ctgaaaaact tcaatagaca acgcataaaa aaccggcatt aacgataatt
     4321 aatgccggtt ttacatttct ataaatgatt atttaaactc ataacgtcta atcgttttta
     4381 gatcgctatc ttcattttta atttcatcgg caagatgaac acgagcacca accgctgcat
     4441 gtgcatcata ctcacgagtt agagtcacag tttcaccatt atctcgttga actgtaagtt
     4501 caacaacatc gtttgatact gctttgctag ataaaactgt tgcattacct ttatcagaat
     4561 aaacatctgc agtacaagct gctaacataa gtgaagctaa tacagctaaa gaaagttttt
     4621 tcattaataa tatatcctct aggaaaaact aaaattaaag cagagaaaca ttcaggtctg
     4681 aattagaccc tacgattctc acacgtttgc caggcacaaa accgtcttct ttcttttgaa
     4741 cgaccacgat ttctttacca tcatctttgc gaataaccat ttccaatgaa gaaacttgac
     4801 tcgctttttg ttctacttga ttaccaatta ccgcaccggc aactgccccc actgcggttg
     4861 caatcgcttg ccctgtacca ccgccaatcg cattaccggc aacgccacct aatacaccac
     4921 cgccgacagt accgatcaca ccttcactgc gtgcttgaat tttcacatca cgcactgaaa
     4981 caatagtgcc ataagcgata gaacgagctt cttttgcctg ctcaccggaa taaacgcttc
     5041 cgctaaacac gtctgtattt gcacagccaa ctaaagcaaa acttgacatt aatgcgacag
     5101 ctaaagctaa ttttttcatt ttgtttgttc tccgaaacgt taattttctg acataattac
     5161 actttttata tcataagggt ggagctttag acaataagga ttacaaaaag ttacatttct
     5221 caagctgatc gcaatgcttg gatttggcaa tctctcccct tctacctgag gttggctgat
     5281 ttttaattta atctcagaat catcagcaag tctaaagtgc gataatgccc ttgacaccca
     5341 ttgttccgaa ctttcgttgt ctaacatcgg aaaaaccagc tcaatatgtt cccagccttc
     5401 ttctaaataa accttatttt tcggaaaagg caactcgaca atctttacaa attgtccgca
     5461 aaattcaacc gcttgtatta atgcaaacag ccctatcggt ctgccgttta cctcgctctc
     5521 tttaagtaag gttgccccat caagcaaaag gatcttccac tgttctgcgg tttcggtttg
     5581 gttcattctt accgcaagat gatcgatttc atacgctgct aaatcaatgc cggcgatttc
     5641 agcaatttgt cggattttct gttcaaattg cgataaatcg ccaaaacaag cggtcatttt
     5701 ctcgaaaaat tttgcatttt ctgctataat tttgtccatt tttttgtaaa tagaatttaa
     5761 ataggttaaa cagtgaatat tcaacaaatt ttatcagata aaattaaaca ggcaatggta
     5821 gcaagcggtg cagacagcaa tgtcgagcct ttagtccgcc aatcgggcaa gccggaattt
     5881 ggcgattacc aagccaatgg tgcgatgggt gcagcgaaaa aattggggat gaatccacgt
     5941 gaatttgccc aaaaaatctt agagaatgcc gatttgaacg gtattgccga taaattagaa
     6001 attgccggtc cgggctttat caacattttc ttaaacccag aatggctggc ggagaacact
     6061 aacagttcat tgcaagcggt caattttggc ataaaaactg caaatccgca gacgattgtg
     6121 atcgactact cctcgccaaa cgtagcaaaa gaaatgcacg tcgggcattt gcgttccacc
     6181 attattggcg atgcggtagt tcgtaccctt gcgtttttag gcaataatgt gattcgtgcc
     6241 aaccacgttg gcgactgggg tactcaattc ggtatgctga tcgcttacct tgaaaaaatg
     6301 gaaaatgagc acgtcagcga aatggagcta agcgatttag aagccttcta tcgtgcggca
     6361 aaagagcatt atgattcaga cgaagcattc gccgaaaaag cccgtaacta tgtggtgaaa
     6421 ttacaaagca gcgataaata ttgcttaaca atgtggaaaa aattggtcga tattacaatg
     6481 caccacaatc aagagaacta caatcgctta aatgttacgc tcaccgaaaa agatgtaatg
     6541 ggcgaaagcc tctacaatcc aatgctgcct gaaattgtgg cggatttaaa agccaaaggc
     6601 ttagcggtgg aagatgacgg tgctttagtg gttttcctcg atgaatttaa aaataaagat
     6661 ggcgacccaa tgggcgtaat cgtgcagaaa aaagatggcg gtttcttata taccactacc
     6721 gatattgcgg cagcaaaata tcgctacgag accttaaaag ctgaccgagc cttagtcttt
     6781 tctgacagcc gccaagctca acatatgcaa caggcatggc tcattacccg caaagcgggc
     6841 tatgtgccgg acagtttcag ccttgaacac ccgttcttcg gaatgatgct aggcaaagac
     6901 ggcaaaccgt tcaaaacccg tagcggtggc acagtcaaac tcaaagattt attagatgaa
     6961 gcggtagagc gtgccgataa actgattgcc gaacgttcaa ccgagctaac tgcggaagaa
     7021 aaagccgccg tggttgaagc ggttgcgatt ggttcggtga aatattctga cttatcgaaa
     7081 aaccgcacca ccgattatgt gttcgattgg gacaatatgc tgacctttga aggcaatacc
     7141 gccccgtata tgcagtatgc ctacacccgc attcgctcca tctttgcccg tgcgaacatt
     7201 gacgaagatg cattgagcgg ttcaatccaa ctcaatgagc caaaagagcg tgcattagca
     7261 gtgaaactat tgcaatttga agaggcatta aacggcgtgg caaaagaagg tatgccacat
     7321 attttatgcc aatatttata cgagcttgca ggtagtttct ccagtttcta cgaggcttgc
     7381 ccgattctca atgccgaaga aacaaccaaa aacagccgtt tgaaactcgc tgccttaacg
     7441 gcgaaaacct tgaaacaagg gttggattta ctcggtatta aaactgtaga gaagatgtaa
     7501 tacaaactct ctccccttgt gggagagaga ccgcttatga gcgttcagcg aaataagcag
     7561 agagagggta caagcggtaa tttttattaa aatttttaca cattttcccc tctccctgat
     7621 ttttcttctg aacgaagaaa aatctgtccc tctcccacaa ggggagaggg catataaaag
     7681 gaaacaaata tgaaatttat tgacgaagcc ctgattcgtg tagaagcagg cgatggcggt
     7741 aatggctgtg tgagcttccg ccgtgaaaaa tttatcccga aaggcggacc tgacggtggt
     7801 gatggtggcg atggtggtga tgtgtattta attgccgatg aaaacctaaa cacccttatc
     7861 gactaccgct ttgaaaaacg ttatgctgct gggcgtggcg agaatggtcg cagtgccggc
     7921 tgtaccggac atcgtgggca agatattacc ctacgagtgc cggttggaac ccgtgcgatt
     7981 gataacgaca cccaagaaat tattggcgat ttaacccgcc acggaatgaa aatgctggtg
     8041 gcaaaaggtg gttatcacgg tttaggtaat acccgcttta aatcctcagt aaaccgagct
     8101 ccacgccaaa aaaccaacgg tacgcctggc gaaaaacgtg atttgaaatt agagctgtta
     8161 ctgcttgccg atgtgggaat gcttggctta ccaaacgccg gtaaatccac ctttatccgc
     8221 agtgtctctg ctgccaaacc aaaagtggcg gattacccgt tcacgacgct ggtgccaagt
     8281 ttaggggtag cccgtgtggg ggccgaccgc agttttgtgg ttgccgatat tccggggtta
     8341 attgaaggtg cagcagatgg tgcagggctg gggattcgtt tcctaaaaca cttggaacgt
     8401 tgccgtattt tgattcactt agtcgatatt atgccgattg atgaaagcga tccggctcat
     8461 aatatttcgg tgattgaatc cgagctttac caatacagcg aaaaactcgc cgaaaaaccg
     8521 atctggttgg tgtttaataa aattgacacc atcggcgaag aagaagctgc tgagcgagca
     8581 aaagacattg ccgagcaaat cggctgggaa ggcgattact acttgatttc tgccgctacc
     8641 ggtcaaaacg tgcaagctct cactcgtgat attatggact ttattgaagc caacccacgc
     8701 gaagtggagg aagaaaataa agcacctgaa gaagtcaaat tcaaatggga agactaccac
     8761 aacgaagcaa tgcaaaatgc ggtagaagaa gattgggacg acgattggag cgaagaagat
     8821 gacgaaggca tagagtttct ttacgaacgc cgttagtgca ataatacaag cggttgtttt
     8881 taaacggaga tttgcaaatt tccggagaaa aacaaccgct tgtaggttta aggagagttt
     8941 atgcttagct caacatcgtg cgatttattc aatattccgt tcttccaatt tgctcagatg
     9001 aagaaatatt gccctgagga gatccctcga attaaggcgg aatataaaac tcaatgggaa
     9061 atttggaaaa agctacatca ggaagtttct cgtcagcttg ggcagccgtt tgctccacca
     9121 catattgaga agtggtgcaa cggttggcaa gttcgagccc atttttttgc ctattacaag
     9181 tatgaattta atcgaaattc tgcggctatt ctttccgtga ttttaaatcg tcgccgtttg
     9241 caagtgagcc ttgattggca ttgttatcga gccgatcgct cgcaaattaa cgtgcatcaa
     9301 tacaatcaat ggtgggaaaa tttagatcgg gaaaagtttg ctgattttga gatttggcac
     9361 ggaagtgaga gtgaatatga tgattttaga accgttagtc agatgtcaga gcaagcgttc
     9421 accctaacta acgaggaaga tttttggtgt atcggcaaaa atgtggaaaa ggacgatttg
     9481 gataacattg atgtagtaga atttatctac caaaccattc gggcattatt gccactttat
     9541 gagaaatgtc atcaataaac actaaaaaga gaggcgaaca tcatttgttc gcccctttca
     9601 aaatttaagc tatcacttct tttttatttt caatgcgtaa acggtagcgt ttatccaaca
     9661 tacagagtaa accaccgaga gccataaaca gcccgccaat ccaaatccag cgaatgaacg
     9721 gtttataata caaacgcact gcccaagagt tatcttctaa tttttcgcca agtgcagcgt
     9781 ataaatctcg ggtaaagccc cagtcaatcg ccgcttcggt cattcccatt cggctcaccg
     9841 tatagaaacg tttttcagca ttaagtgtgg cttcatattt accgtttcga gtaatttcaa
     9901 gctcggcagt gccgccttga tagtttgccc catcggtgat tttaatacct ttaaattcaa
     9961 attggtaatc taaaatctca gctttatcac ccacattcat tctgacatct cgttcaatgc
    10021 tgaaattttg gctaaatgca atgccgaaca ctgtcatcgc aacccctaag tgagccagca
    10081 ccattcccca atgtgaacgg gacagtttga aaatgccact taagaacgag tggcgatggg
    10141 ttgcacgttg gtgtagctca tacaggctaa gtaacacaat aaagacagac atcatcgtgc
    10201 ctaacaccac actcacggta atggtgttac ggaataagta tggcaatccg aatccaagca
    10261 atgccattaa aatagttgca ataattaccg gtttacgaat agcagaaact tggtctctac
    10321 gccatttcac taatggtcca atccctaata caaatgagaa aggaaccatt aaaattaaga
    10381 acatttggtc aaaaaatggt gccccgattg aaatcgtccc taagccgatt tgtttgtgaa
    10441 ttaatggcag taaggtccct aaaaacacca ccgataaaaa tgccattaat aaaatattat
    10501 taatgagtag catcgtttct cttgaatagc gttcatagtt atctaagctt ttaatttccg
    10561 agcctttgaa tgcatacagc agtaaagaac cacctactac taacaccaag taagctaaga
    10621 tgtataaacc acgagttgga tctgaagcaa aggcgtgaac tgacaccaaa atgcctgaac
    10681 gcactaagaa agtccccact aaacagagcg agaaagcaag aatcgctaat aagaccgtcc
    10741 acgctttaaa tgtgcctcgt ttttcagtca ctgctaatga atgaatcagt gctgttcctg
    10801 cgagccacgg cattaatgat gcattctcga caggatccca aaaccaccag ccaccccaac
    10861 caagttcgta gtatgcccac caagaaccaa gcacgatacc gagcgttaaa aatacccacg
    10921 ctgccattgt ccacggacga gaccatcttg cccacgcagt atctaaacgc cctgtcatca
    10981 gtgaggcaat cgcaaaagca aaggcaacgg agaaacccac atagcccata taaagcaacg
    11041 gtgggtggaa gatcaagccc acatcttgca gcattgggtt taattctttg ccatcaatcg
    11101 gaaaatttgg gaaagttcta tcaaacgggt tggaggtaaa aatcacaaac atcaaaaagc
    11161 cgatgccaat gacccccatt agtcctaaca ctcgagcaac ggcatcttcc ggcattttac
    11221 gggtaaaggt tgctaccccg acagaccaaa gcgacaacaa ccaaacccaa agcaataatg
    11281 agccttcgtg agcgccccac accgcagata aacgatattg caacggtaaa cggctattgg
    11341 agttgttcac cacatattgc acggtaaaat cattcaccgc aaacagataa aacagcgaac
    11401 caaaagagac agtcaatgcc aaaaacattg cccaagtcat cgggcgggca agagcaatta
    11461 aggtgctatt ttgcttttct gctccccaaa gcggtaaaac tgccaacaag atagaaatcg
    11521 ctagggctaa tgccaacgta taattgccta attctgcaat catttgcttt ccccttgcac
    11581 attgttggct ttttctgctt ccgcctcggc tttcattttg cggtcggttt ccgattcacc
    11641 tttcagatct tgttcagaca cacctgattt tttatgctgt tcttttaatt tatcgcctaa
    11701 ttccggtggc atatagtttt catcgtgttt tgccagaact tccgtagctt ttaaacgggt
    11761 tagttcaatc aacacgcctt gtgccacaat gccttgccct tcacggaata aatccggcaa
    11821 aattccttcg tattccacgg taatcgaagg cccgatatca ttgagatcaa atactacttt
    11881 taaactttta tcatcacgct ttaccgtatc ttccactacc attccgccca cacggatacg
    11941 ctgaccaact tcaggctttt gtttcggatc atcattttta ccgtttacaa tttcagacgg
    12001 cgtgtaaaac agatcgatat tttggcttaa cgcataaagt gtcaagcctg ctgcgacaga
    12061 aagcccaagt aatatcgaaa ccaccacttt taatcttgat ttgcgtcttg gattcatcgt
    12121 aatcaccctt caaaataaat gcaaaattgc ataaaaactc aaaaataaat tagtgtaaga
    12181 atagcaaaaa accgcaaaac attcccgata tttcaaggaa gttttgcgat ttctcaaatc
    12241 aaatacctat aaagaagtat gaggctatcg ttgcaaaaaa taaagttatt tcgaccgctt
    12301 gtttattgtg atgtttgctt taatttttct tcacgcacaa tctctttttt tgcttgagcc
    12361 aaaatcgctt tctgttcacg gcgactaagc caaatcagcc caatcattgc aatgaaactg
    12421 acaccataag agagccatac ataaaagccg tagccaccca ttgcaaaaaa atcgctgatt
    12481 gattcaaatt gaaatgtcat tgttctctcc tactctaaac tgattttgcc agttgtttta
    12541 cccaagggcg attacgttcg tcctttaata aggcaatgcg ataacgccaa atgctaaacc
    12601 aagcggtaaa aattaagcta ccgaaaatcg ccaatagcaa cggaattaac atttccaccg
    12661 ccatagacgg tttatcgaat ttagtgatgg tcgccccttg atgcaaggtg ttccaccatt
    12721 ccaccgaaaa gtgaataatc ggcaagttaa tcacgccaac aactgacagc acagccgccg
    12781 ctttggctcc ggtttgtctg tcttggaatg cggaataaag agccatcacg ccgatataaa
    12841 ggaaaaataa cactaaagcg gaggttaaac gagcgtccca cacccaccaa gtcccccaca
    12901 ttggtttgcc ccaaattgca ccggttgcaa gtgaaataaa agcgaatact aagccgatag
    12961 gtgccattga aatcatcgcc aagttagctt ggcgaatttg ccaaaccaat gccaccaagc
    13021 ctgccaccgc cattgagcca tatacgccca tcgaccaaat tgctgccggc acgtgaataa
    13081 agataatgcg atagctatcg ccctgttggt aatctttcgg ggcaaaagcc aagccccaaa
    13141 caaaagcagc cgctaacata atcaaactta gccaacctaa aaacggttgg attttcccta
    13201 gcaaatggta ctgcgtttcc gattttgcat aagggtgtag ccatttccac atatatttct
    13261 ccaaagtaaa taattcccct cacccgttta caggagaggg acgatttgag cttcgttcag
    13321 aagcgaaaat cagggagagg ggaaaaagtt gcaaattttc ataataattc gaccgcttcc
    13381 cctctctctg aaaaacacct ctgaacgagg tgtttttcgg tctctctccc tcaagaggga
    13441 gagaggaagt tttagttcaa actaattctt aatgcaccgg caatcgcaaa cggcgaaaaa
    13501 gttaaagtaa ccgctaaaat tgctccgata atcgccagtt gtccgctata acccatattt
    13561 aaggtggcgg cttctaatac cgcagcggca aaaatcagta ccggcaagaa taacgggagg
    13621 attaataaac tcagcaacgt accgcccttg cgtaagccca cagtgagtgc cacgccaatc
    13681 gctcctaagc agcttaaaat cggggtgccg agtaataatg tcagcactaa cgcccaccaa
    13741 acgtgggttt caagtgaaag caaaatggca gcaatcggtg aaagtaaaat caacggtaag
    13801 ccggtcagca gccagtgagc aatcactttc gctaacgcaa cctgcggcaa ggcaagcggc
    13861 agtaacatta attgctcgag cgaaccgtct aaataatcat cacggaataa ccgctcaaag
    13921 gaaagcaatg cagaaagcaa agcagctacc cacgcaatgc cgccggcaat tttgcctaat
    13981 aattcggggt taggtcccat taaaatcggg aatacggtaa tcaccattaa aaaaaaccac
    14041 aacggattta aaatctccga gggtttacga aaggcaatgg tgagttcccg ctgaataata
    14101 gtcaataaaa tcatacttta aattgatcta acgaaataat acgcactttg tcgctttcag
    14161 cagcctggtg gctggtaaaa atcactaatc tgccttgctc gcaatgctgt tcaatatgag
    14221 caatcagatc cgccacaccc tttttatcaa tagcggtaaa gggttcatct aaaatccaaa
    14281 gtttggcgtt agtgagccac aattttgcca acgccacacg gcgttgctga ccggctgaca
    14341 aatgcgaaca aggcaaatct tcacgcccga tgagcgacac tttcgtcaat gcgtgccaaa
    14401 gggcatcatc atcttgcggt aactgttgga ttttctggta gaaacgcaaa ttttcccacg
    14461 gtgttaattc cggttttacc cccgaataat gccctaaata aaatagttca gaataatagt
    14521 gatcacgctg tttttgaatt gcaatatcgt tccacagcac ctcaccttcc gcaggtaaag
    14581 aaagcccggc taaaatacgt aacaggctgg tttttccaat gccgttatgc ccttcaatct
    14641 gcacccattc accactttgc acggtaaaac tgcacccttc aaacaaacgg gtttcgcccc
    14701 gttcgcaggc aatctgatta agtttaagct gatattgggc cgtttggttc attatttttc
    14761 atctggttga acattttagc cgaagtgtat cataagcctt tgatttaacg tagcaaataa
    14821 attacttctt tagaggtagc aagcggtcaa attttcaaaa agttttgcaa attttgataa
    14881 gaatcaaacc gcttgttaca gagagaggat ttccttcaca aaaggaatgg ttaatttacg
    14941 ctgggcttgg agagacgctt tatccaactt atccaatatt ttagagagtt gctctaaatc
    15001 cgttcccgct ttttttaaca gaaaatgagc gacctcatcg gacaattcca cccctttttg
    15061 gtgagcattt aactgtaaaa tttggcattt ttgttcatcg gttaaatcgt ttagctggta
    15121 cacttcgccc caagttaagc gggaacgtaa atccggcaat tggatattga gttggtgtgg
    15181 tgggcaatcg gcactgatta acagcaaggt tttttgccct tcgttaaaca agccttgttg
    15241 ttctcgaatt tggttaaaca ggttaaaaat agcaagttcc cattcagcat ctcctgcaat
    15301 cgcttgaata tcatctaggc aaatcacatt cagcagttcg gcattatcta acaccaacgg
    15361 cgagaaataa ttggctttag tgagcggaat ataactagaa gtttggttat ttaaaagaaa
    15421 gtggttactt actgctttca acaaatgact tttaccgcag ctcttaccgc cccaaatgta
    15481 aaaaaacggc tgttgaacat ccgtaaaatt tcgtttgagg gattcaagca acacttcgct
    15541 gttttggcta taaaaattgt cgaaagtgtc ttcatcaatt tgatgaatgg gtagaggtaa
    15601 ctgcaaggct ttccttaaat ctctaagttt gatatttttc taagaatagc ggaaaagcag
    15661 gggtttgaga agtaaaaata gtgagaaaat atccactcat ttttctaatg aagagatgac
    15721 aagcggttga atttccacat aattttgtca atctgaccga aaacccaccg ttttgtgtta
    15781 aaaaaagatt gcagttagct caaaaaattg tatattcaaa gctttctact taaaaagtag
    15841 agtaaaacaa ctattacaaa cacaacggag tgaatatgaa aaacatgact aaattatcgc
    15901 taataacatt agcgctagcc gcttcaagta gcgtctttgc tgcaccgaaa acctttgttt
    15961 actgcttaga ggcttcgcct tcagcattaa gcccagtgct atcaacagac ggtgcaagct
    16021 ttgatgtgaa cgccctcccg atttataaca aattaaccga ctttgaagac ggtacaacta
    16081 atgttattcc ctctcttgcc gaaaaatggg acatttcgga agacggcaaa acttacacct
    16141 tccacctgcg taaaggcgta aaattccacg acaataaaga atttaaacca agccgtgagt
    16201 taaatgcaga cgatgtagtg ttctccatta accgtcagtt tgatccaaac catccattcc
    16261 acaaagtttc cggcggtaac tacgaatact ttatcggtat ggatatgcaa aatattatcg
    16321 ataaagtgga aaaagtggac gatcacaccg ttaaaatcag cttaaaagtg ccaaatgcgc
    16381 cgttcttagc taacttagcg atggatttca acgccgttta ctcagcggaa tatgcagagc
    16441 aatccttaaa ggccggcaag cctgagcgta ttgataccaa cccaatcggc agcggcccgt
    16501 tccagtttgt ggattaccaa aaagatgcca ccgttcgtta caaagccttt gaaaactact
    16561 ggcaaggcaa agcgaaaatt gaccgcttag tgtttgcaat tactccggat gcatcggtac
    16621 gtttagccaa gttacaaaaa ggcgagtgcc acgcaatgcc ttaccctaac ccggctgata
    16681 tcgaaaatct gaaaaaagat ccaaatattg aattaatgag ccaatctgga atgaatatcg
    16741 gctatttggc attaaattca tcgattaaac cattagataa ccaaaaggta cgtcaagcat
    16801 taaatcacgc tgtaaacaaa caagcgattg tagatgcggt atatcaggga gccggtcaag
    16861 tagcgaaaaa cccaattcca ccaacaatgt ggagctataa tgaagcggtt caagactatg
    16921 actacaaccc tgaaaaagcc aaagcattgt taaaagaagc cggttttgaa aatggttttg
    16981 atactgaact ttgggcaatg ccggtatcac gtccatataa cccgaatgct cgccgtatgg
    17041 ctgaacttat ccaagaagac tggaaaaaag tgggcgtgaa tgcgaaaatc gtcagctatg
    17101 aatggggcga atatctcaaa cgcttacgcc aaggtgaagc ggcaacctca atgattggct
    17161 ggacaggcga taacggtgac ccagataact tcctcaacac cttattaagc tgctcagcgg
    17221 tggaagccgg ttcaaactac gccaaattct gccacaaacc gtttgaagaa gtcgtgcttg
    17281 gtgctgccca agaaagcgac aaagcaaaac gtaccgagct ttataaacaa gctcaagtga
    17341 tcttcaaaga gcaagctcct tggattacga ttgctcactc caccgtttac ttcccggtgc
    17401 gtaaagaagt aaaaggttat gtgatgagtc cgttcagctt acataacttc tacaaagtag
    17461 atttagcgga taaataatcc gccaacaagc ggtagaattt gcagattttt ttacaaattt
    17521 gaccgcttgt cctcccctct ttgaactacg aggggcaggg gggagatttg gcagaagcga
    17581 ttgccgaagt gagaggattg taaaagagga aataaatttt ctgtcttatt gattattttc
    17641 ttcgttaatt actactgtca aatctcccct accccctctt tgctaaagag gggaatcaga
    17701 tcgagagatc agacaacttt gatgaaaatc caacctaaga gattttaaat gttttcattt
    17761 attttaaaac gtgtgctaat ggtgatccct acctttatag cgattacgtt aattactttt
    17821 gcccttgtgc attttatccc cggtgatcca attgagatcc gaatggggga acgtggatta
    17881 tcgcctgagg ttcatcaaca aatgatggaa caactcgggc tgaaccgacc gttgtttgaa
    17941 caatacatca gctacatcaa aggtgtattg cacggtgatc ttggtaactc atttcgtaac
    18001 aacgaaccgg tgctgaagga attttttacc ctattcccag ccacggttga gttggcattt
    18061 tttgccctgc tctggtcgtt gattttcggc attattctcg gtgtgattgc tgcggtgaaa
    18121 aaagaaagtt ggatttcaca cactgtaacc acgctttcac tgaccggcta ctcaatgcca
    18181 attttctggt ggggcttgat tttaattctt tatttctcca ccccgctcgg cttaccggct
    18241 tcaggtcgtt taccatctga atactggatt gaagccgaaa ccggttttat gctgattgat
    18301 acttggaatt cggacgaacc aggggcattt ttagcggcaa ttaaatctct gattttgccg
    18361 tctatcgtac tggggacaat ccctcttgca gttgtgaccc gaatgactcg ctcttcaatg
    18421 ttggaagtgt tgggtgaaga ctatatccgc accgccaaag ccaaaggctt aaacactacc
    18481 cgaattgtga ttgttcacgc attaagaaat gccttgattc cggtggtaac ggtcgttggc
    18541 ttaattgtcg ggcaactgct ttcaggagcc gttttaacgg aaaacatctt ctcgtggccg
    18601 ggtatcggca aatggattat tgatgcaatc aacagccgtg attaccctgt actacaaggc
    18661 tcagtgctga ttattgctac tattattatt ttagtgaacc ttacggtaga tctactctat
    18721 ggcattgtca atccgaggat tcgacatagt tagaaaaatt ccttcgattt ttcccctctt
    18781 tagcaacgag ggagttacac aagcggttca aattccgcaa ttttttacaa gattattttt
    18841 agttggagtt aatgatgtca ggaataacgg cggtaaatcc acccgcaccc aaaacaccgt
    18901 tacaggagtt ttggtactat ttttgtcaaa atcggggggc attaatagga cttaccttta
    18961 ttgcaatcgt ctttgttgcg tgcgtattcg caccttgggt tgcccctttt gatccaatcg
    19021 aacaaaatcg tagtgcgtta ttgctccctc ctgcttggct tgaagggggg aattccgctc
    19081 atctgctggg tactgacgat attgggcgtg atattctttc ccgtattatt tatggttcaa
    19141 gattgtccgt ctttatcggc ttagtcattg tgctactttc ttgcgtattc ggcgtgattt
    19201 taggtctact tgctggctac tatggtggca cactcgacat catcattatg cgttttgttg
    19261 atattatgct ggcgattcca agcctgcttt taactatcgg cgtggtcact attttaggcc
    19321 cttcattgat aaatgccgct attgcgattg cgattgtctc cattccaagt tatgttcgct
    19381 tgacccgtgc ttctgtatta agcgagaaaa accgtgatta tgttactgct tctcgtgttg
    19441 ctggtgcgag cgtgcttcgc ttaatgtttg tggtgatttt acctaactgt ttagctccgc
    19501 ttatcgttca gatgacgatg gggatctcaa acgctatttt agaacttgcc gccctcggct
    19561 tcttaggcat tggtgcacag ccgccaacac cggagcttgg cacaatgctt gccgaatctc
    19621 gtggctttat gcaatcggca aactggctag tgaccattcc gggtctagcg attttatcgt
    19681 tagtattagc tttcaactta atgggtgacg gcttgcgtga cgccctcgat ccaaaactga
    19741 aacaatagga gatctaaaat ggcattatta acggttgatc aactttccgt ccattttggt
    19801 gaaactaaaa caccatttaa agcggtggat cgtatcagtt acgaagtcaa tgaaggcgaa
    19861 gtattaggca ttgtgggcga atccggttca ggaaaatcgg taagttctct cgctattatg
    19921 ggcttaatcg atttccccgg cagggttatg gctgaaaaat tgtattttaa cggcaatgat
    19981 ttattggcat taaaaccaaa agaaaaacaa cagattgtag gggctgatgt ctcaatgatc
    20041 ttccaagatg caatgaccag cttaaatccg agctatactg tgggctatca gattatggaa
    20101 gccctaaaag tgcatcaagg tggctcaaaa gccagccgtc gtgagcgagc gattgagttg
    20161 ctgacgatgg tcggtattcc cgatcctgaa tcccgtttag aagtttatcc gcaccagctt
    20221 tcgggtggta tgagccagag ggtgatgatt gcgatagcga ttgcctgtcg cccgaaatta
    20281 ttgattgccg atgagccgac tactgcgtta gatgtgacga ttcaagctca aatcattgat
    20341 ttattacttg aattacaacg caaagaaaat atggcgttga ttttaatcac gcacgattta
    20401 gcgttagttg ccgaatctgc ccatcgcatt atcgtgatgt atgcagggca agtcgtggaa
    20461 gagggcaaag ccgagcagat attcaaagca ccactgcacc cttacaccca agcattgctc
    20521 aaagccttac cggaatttgc cgaagggaaa tcccgcctgc aatcactacc gggcgtagtg
    20581 ccgggcaaat atgaccgccc acaaggttgc ttactcaacc ctcgctgccc gtatgcaacc
    20641 gaaaaatgcc gaaccattga accggcaatg cagatgatca acggcagaca ggtgaaatgc
    20701 cacacgccac tgaatgcaca gggtgagccg ataaggatag ctgtataacg tcataaagag
    20761 tagggacgca atgcttcgcg tccgttacaa gcggtgattt ttaaccgatt tttgcaaaat
    20821 aacgatagtg gacgcaaagc attgcgtccc tacattttcc gagaaggaaa acagaatgaa
    20881 agaaaataat tctctcccac cactcctcaa agcagtggac ttaaaaaaat actacccagt
    20941 taaaaaggga atgttcgcta aaccgaaaac ggtaaaagcg ctggatggtg tctcttttac
    21001 ccttgaacgt ggcaaaaccc ttgcgattgt tggcgaatcc ggctgtggaa aatcaacttt
    21061 ggggcgaatg ctcactatga ttgaaagccc aaccgaaggt gaactgttct acaacgggca
    21121 gaatttctta gtcaatgaca aagaaacggc tcaactacgc cgtaaaaaaa tccaaattgt
    21181 gtttcagaac ccgtatagct cgctcaatcc acgcaaaaaa gtaggtagca ttttagaaga
    21241 accattattg attaacacca acttatccgc aaaagaacgc aaagaaaaag tgttggaaat
    21301 gatggcgaaa gtcggcttaa aaccggaatt ttacgaccgt tatccgcata tgttttcggg
    21361 cggacaacgc cagcgaattg cgattgctcg gggcttaatg ttgcagcctg atattgtggt
    21421 tgccgatgaa cccgtttccg cattagatgt ttcggttcgg gcgcaagtgt tgaatttgat
    21481 gatggatttg caagaagaaa tgggcttgtc ttacgttttc atctcgcacg atttatccgt
    21541 agtggaacat attgccgctg aagtgatggt gatgtattta gggcgttgcg tagaacaagg
    21601 ggataccaaa actatcttca gcaatccacg tcacccttat acacaagcgt tactctccgc
    21661 aacccctcgt ttgaacccgg agcaacgccg tgagcggatt aaactgactg gcgaactacc
    21721 aagcccgctt aatccaccaa aaggctgtgc ctttaacgcc cgttgcagat ttgcaaccga
    21781 tcgttgctat gtggagcgac cagagctgaa aacctatcaa gacggtactc gcatggcttg
    21841 ttttgtggtg gaggaaaata attaggcaac gctcgctatt taaaaggtta aagtttttca
    21901 actttaacct ttatttttca tacttaatgc tcctcatctt ttgtagcaaa atctttcatt
    21961 aatttgctga tatgctcaca atggtgttta aattgacgac aatacgggca aataatcaaa
    22021 tgcgttttaa cgccaatctt ctcttttaaa gtgagttttc gtttctttga gtcagaaatt
    22081 aaccttgtta cttgtcggca attcatctat ttctctccgt taatttcctt gaaagacatt
    22141 gttgtaactg cagccttgct cgatagatta atgtatataa attatctgat gtaatattta
    22201 ctactcgaca aatttcatct gaggttgcct ctaaatattc cctcatcata aataccctag
    22261 cttgttttgc aggtaaattg cttaagcaaa cttcaaataa tatccaaaat tctttggaat
    22321 aaacagcttc ttcctaccag ctagaaggat aatattcttc tttccaatgc ccataattat
    22381 caaaaaagtg attctcttcg tcatcatcag ctagatcact ttctagtact atacgatctt
    22441 ttttacgtaa ataatcaata attttatttt ttagaatagc aaatatccag gttttcaatg
    22501 ctgctttacg ctggaaatta tcaatatttt ttattgcatt aacgaatgct tcttgaacca
    22561 tatcttcagc aagagcaata tcttgtaatt gtaactgagc aaatttcaac atttgctgac
    22621 gaatatcggc aagctgttcc gatgaaatat ctaaagaaat cttgttatga atcatagtgt
    22681 tatattgaga aaattttttg tgagaatatt aaacaatatt cgactaatcc tatagagagt
    22741 aaaaccaatt ttgatcttag caaaaaagta agaaattatg aatgtttaag gttaaaccct
    22801 cgtccttaaa aaataaaatt acataagtta taaaggagaa ctgaatgaaa tacaatcaac
    22861 tgttactatg tttggtgtct gctatcttta ccacctcctc aatagcaaac atcacaaccc
    22921 ctaacgagat gcgatctgaa aacttacagg atatttactt ggcaggagga tgtttctggg
    22981 gcattgaagc ctatatggaa aaaatatatg gcgtaaaaga ggctacatcg ggctatgcca
    23041 atggcaaaac tgagcaaact gcttaccgaa ttatcgggca aaccgatcat gcagaaacag
    23101 ttcacgtgac ctatgatgct aataaaattt ctttagaaaa gctgttgcaa tattacttta
    23161 aagtaattga tccaaccagt aaaaataaac aaggtaatga tattggccgc caatatcgga
    23221 caggtattta ttatcagaat gagcaagata aacaaattat tttaaaagaa atacaagctc
    23281 aacaagcaaa atacaaacaa aaaatacaag tgcaagtaga accattaaaa cattatattt
    23341 tggcagaaga gtatcatcag gattacctaa agaaaaatcc aaatggctac tgccatattg
    23401 atttaaacca agccaacagc attattatcg atccgaaaga ttatcctaaa cctagtgatg
    23461 caacattaaa aatgaaatta acgcctttgc aatatagcgt tacccaacgt aaaaatactg
    23521 agcgttcatt cagcaatgaa tattgggata ataccgcacc gggcatttat gttgatataa
    23581 ctacaggaga gcctcttttt tcatctctcg ataaatatga ctccggctgt ggctggccaa
    23641 gttttaccaa accaattatc aatgaggttg ttaactatac cgaagataat agttttaata
    23701 tgacaagaac ggaagtacta agtcgaagtg gtaacgctca tttaggacac gttttcactg
    23761 atggcccgaa agataaaggc ggattacgtt attgtatcaa cagtgcctca atcaaattta
    23821 ttccattaga gcaaatggaa aaagaaggtt acggctattt aataaatatg attaaaggaa
    23881 ataaataatg atgaagaaaa taattagttt actcataatg atgttgagtc ttaacagcta
    23941 tgcacaagaa aactctcatc ttgggcatat tcctcttaaa gatctcaatc aaaatcaaat
    24001 tactcttgat aaatatcaag gtaaaaaaat ctatataaaa gcatgggcat cttggtgttc
    24061 tatttgtcta gccggattgg cggaaattga ggaaataagc caaaaagcaa ataacgatat
    24121 tgaaattatt actattgttt caccagggca taaaggcgag aaaacaacca atcaatttat
    24181 tgaatggtat aaaggaatag aatctgatta tcctaatatt actgttttat tggatgaaca
    24241 aggagacttt attaaacaaa ccgaaataag aggatatcca cataatttat tgttagattc
    24301 ttcactcaat ttaaagaaaa gtattccagg tcatttaaat agtgaacaaa tacttcattc
    24361 tattactaac tgataaaata aggggctata taaacgatag ccctt
//
LOCUS       DANKBV010000024        24044 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.024, whole genome shotgun sequence.
ACCESSION   DANKBV010000024 DANKBV010000000
VERSION     DANKBV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24044
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.024"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            352..1542
                     /gene="malE"
                     /locus_tag="PWY43_000750"
     CDS             352..1542
                     /gene="malE"
                     /locus_tag="PWY43_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDL2135649.1"
                     /translation="MKNKCVKFTLATLATLVLSGTVMAKMTEGKLVIWINGDKGYNGL
                     AEVGKKFEKDTGVQVLVEHPDRLEEKFAQVASTGDGPDIMFWAHDRFGGYAQSGLLAE
                     VNVSKEFKDKFVDFAWDAETYNGKIIGYPVAIEAISLIYNKDLVKEAPKSWEEIIELD
                     KKLKKEGKNAIMWNLSEPYFTWPIAASNGAYAFKFANGKYDPKDIGVNNEGAVKALQF
                     VVDMVKNKHISADMDYAVAEASFNKGQSALTINGPWSWGNIDKSGIKYGVTLLPTLNG
                     QASKPFVGVLSAGVNSSSPNKDLAKEFLENYLLTDDGLDAVNKDKPLGAVALKSYQEK
                     LAADPRIAATMANAKNGEIMPNIPQMSSFWYAEKSAINNAVTGRQSVKEALDDAHSRI
                     QKQQ"
     gene            1661..3199
                     /gene="malF"
                     /locus_tag="PWY43_000751"
     CDS             1661..3199
                     /gene="malF"
                     /locus_tag="PWY43_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDL2135650.1"
                     /translation="MLTQTQSKSTHWLKYLLAGLVLLLDFYLVVLMYSQGEYLFAILT
                     LIILTSGVYIFTNKNTYAWRYVYPGITGMAIFILFPLVATIAIAFTNYSGSNQLSFER
                     AVSVLTEQRYFAGDKYQFTLYPQADNKYQIALTNPTTEQTFVSEPISLATGTNVVVTE
                     QNRPTGEVAPLKVITQNRAALQSMKVVLPNNNELTMSSLRQFSEQKPRYQFDEENKIL
                     LNNETGKRYQANDETGFFQSIDENGHWQSETLEPGYTVTSGFNNFIKIFTDEGIQKPF
                     VQIFIWTVIFSLLTVVFTVILGMVLACLVQWEALKGKAVYRVLLILPYAVPSFISILI
                     FKGLFNQSFGEINMILNQLFGISPEWFNDPLLAKVMILIVNTWLGYPYMMILCMGLLK
                     AIPADLYEASAMDGASTWQNFSKITFPLLLKPLTPLMIASFAFNFNNFVLIQLLTNGR
                     PDMIGTTTPAGYTDLLVSYTYRIAFEGSGTQDFGLAAAIATIIFLLVGGLALLNIKAT
                     KMEL"
     gene            3252..4142
                     /gene="malG"
                     /locus_tag="PWY43_000752"
     CDS             3252..4142
                     /gene="malG"
                     /locus_tag="PWY43_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDL2135651.1"
                     /translation="MAIVQSKSVRYRVWATHFILICFLALIIFPLLMVIGISLRPGNL
                     AIGDIIPSQISWEHWQAALGFDVTHADGTVTLPPFPVLRWLWNSIKVATITSIGIVTL
                     STTCAYAFARMKFKGKKTILQGMLIFQMFPAVLSLVALYALFDRLGQYVPFLGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDGSLEEAASLDGATPWQAFRLILLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLYPQNYLWGDFAAAAVLSAIPITL
                     VFLLAQRWLIGGLTAGGVKG"
     gene            4217..6274
                     /locus_tag="PWY43_000753"
     CDS             4217..6274
                     /locus_tag="PWY43_000753"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDL2135652.1"
                     /translation="MKNALPYLCLFYTSSLFAQSWQHSHFSDFNDNLEKRLYQSQVQL
                     EKGSYPLRFKFGEQCYQPQQAIKLNQSVALVPCINEAPQLRLFRQGNYLAQIDLRSGT
                     PTLTISVEQQLQHNEAMFQSCPNWDKKPIEIDVSSTFAEGESVSDFYSGNTAIVKNGK
                     VTLMPNENASGLILLEKSNTENTATFDWKNATVYFVLTDRFYNGDPTNDHSYHRQKDG
                     MQEIGTFHGGDLKGLTEKLDYLQQLGVNVLWISSPLEQMHGWVGGGDKGDFPHYGYHG
                     YYHLDWTKLDANMGTEDDLSRFVQQAHQRGIRVLFDVVMNHTGYATLADMQEFNFGGF
                     YLTPDEISRTLGEKWTHWKPKSGQNWHSFNDFIRFNDNQAWQNWWGKEWVRADIADYD
                     SPKFDDLKMSLAALPDLKTESEQAVKLPEFFANKNTNAKTLPNAKVRDYLIGWLSDWV
                     RKYGIDGFRVDTAKHVEKSTWLVLKQSAQQALNEWQKANPNAGFNDRFWMTGEAWGHG
                     VFKSDYYQNGFDAMINFDFQDQAKQGLNCFAHIEPIYAEMNRKLSDFNVLSYLSSHDT
                     RLFFHSDSEQNIEKHKTAANLLLLSPGAVQIYYGDESGRTFGATGSDLIQGTRSDMNW
                     QELQQDPQKQALHQHWQKLTQFRQRHPAIGAGVHQLIKNDSYFAFKRVLNEDKVMVVW
                     AGN"
     gene            complement(6388..9114)
                     /gene="malT"
                     /locus_tag="PWY43_000754"
     CDS             complement(6388..9114)
                     /gene="malT"
                     /locus_tag="PWY43_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDL2135653.1"
                     /translation="MLIPSKLVCSFRLQNSIPRKRLIQELDKSCSYPVVLINAPAGYG
                     KTTLVSQWIENKKNIGWYALDEGDNNSDRFATYFSAALHSAINEEIDVLLEENRKANL
                     LALFNQLLIKAAAFSEHFYLAIDDYHIIENDEIHEALKYWIKHQPSNMTLILISRSIP
                     PLSIASLRVQEQLLEIDINQLMFDHQESTAFFQARLGSELNQQDVIALCNEVEGWPTA
                     LQLISLFAKNKSHTLQVPLQDIAKRLAKSNHFHINEYLADEVLNKVDEETRLFILRCS
                     VLHSINESLVQAVTGEQNSRTKLESLEKQGLFLQQMANSKWQSSDDSWWKFHPLFASF
                     LNLCCQNELYAELPQLHQKAANAWLQLGYVTEALHHAMQLSDTGLLLNILSKHAWTVF
                     HQGELKLLEESLNCLDYAHLTEHTNLVLLKAWLVQSQHRHIEVGGILADFSRALTENK
                     IELSKTANAEFNVLRAQVAINSGDENTALQLASEALNDLSENAYYAHIVATSIIGEAH
                     HCHGNLAEALAMLQKAERMARQHHTYHNILWSQLQQSEILLAQGFSQAAYEMLDKASE
                     FVKENHLQKVPMYEFLLRSKGKILWEWYNLDKAESMAVAGMNALSKFEDKLQCLTLLT
                     KISLARGNLDNTARLLGEVEQLEHSHSYHHDWIANADQVRIFYWQMTNDVSAVRNWLI
                     QNPSPVSDKNHFTQVQWRNIARSRILLEEYHEAKAILDNLIETAEKFSLISDLNRALI
                     VRNRLYFLQGEKELAQQDLIQALKLTRQTNFISAFVIEGDVMAQQIRHLLQLNVLDEL
                     VLHKAQFILRNINQFYRQKFAHFDENFVNQLLKNPKVPELLKISPLTQREWQVLGLIY
                     SGYSNEQISDELQVAATTIKTHIRNLYQKIGVTNRNEAISYTKELLMLMGYH"
     gene            9278..11647
                     /gene="malP"
                     /locus_tag="PWY43_000755"
     CDS             9278..11647
                     /gene="malP"
                     /locus_tag="PWY43_000755"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDL2135654.1"
                     /translation="MTFNSIVEKYCRYFDVTDPKHFTLQQWYQVVAEGSLELIYSQPA
                     TKPTESRHVNYLSMEFLIGRLTGNNLMNLGYYEQIRDYLKQYQVELVDVLEQERDPAL
                     GNGGLGRLAACFLDSMATVGQNATGYGLHYQYGLFKQSFKEGMQKEAADTWARDSYPW
                     HRFNPSKTRHIGFGGKIKHIQADQYEWQPKLTIQGKAFDLPIVGYKNNIIQPLRLWQA
                     DSDQSFDFDAFNEGKFLSADKTIVNAAALTQVLYPNDNHQAGQKLRLMQQYFHCACSV
                     ADILDRHISEGYSLEDLAKRQVIQLNDTHPTLAIPELMRVLLDEHQFTWEQAWDICSN
                     TFAYTNHTLLPEALEQWDQRLFKQLLPRHYQIVEKINDIFHDKVQAEFGDDAKIWEKL
                     AVLFDYRVRMANLCVVTCFRVNGVAEIHSDLLVTDLFPEYHKLFPTKFCNVTNGITPR
                     RWIRQANPQLSDLLDRTLKQDWTHNLELLKGVEQYADDTGFREEYQRIKRNNKVVLAN
                     EIHKTLGLNVNPNAIFDVQIKRFHEYKRQHLNLLNIIATYQSLKANPNQDYTPRLFLF
                     SGKAAPGYYLAKNIIHAINNVADIINNDKQVNDRLQVAFLPDYRVSLAEKIIPAADVS
                     EQISMAGKEASGTGNMKLALNGALTLGTLDGANVEIADMVGEENVFIFGHTVESVREL
                     LAKGYQPKAYYNQDPILKNAVDFLATGKASNGDKEAFKLMLDSLLERDPFLVFADFDS
                     YRQAQEKIGVAYLDQETWLRSAILNTARLGAFSSDRSIKDYQQRIWLNR"
     gene            11729..13807
                     /gene="malQ"
                     /locus_tag="PWY43_000756"
     CDS             11729..13807
                     /gene="malQ"
                     /locus_tag="PWY43_000756"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL2135655.1"
                     /translation="MKITAKQYYRAGIMPYFFDERGVKKTASEKIKHSLFKAFNGNTQ
                     ARETPIPTVNIFYQHQPCFLPFHLADKKHPLTGTWQLQLENSNQIISGKIKARGITLP
                     QDLPLGYHQLQLKTVDKTFNCTIIIAPERCYQPQAVIEQKRLWGTFLQLYTLKSAHNW
                     GIGDFGDLKDFIQQIAPYQADFIGLNPIHSLFPANPNSASPYSPSSRRWLNIAYIDIN
                     QLAEFQQSDEAQTWFHSAEIQQQLSELRTQDWLNYEQIIPLKLKGLRFAFAKFQQNMT
                     ACAAPAFIEFVQKGGESLQVQATFDALHQHLSAQFADQWGWDFWEKAYQDYHSAAVQQ
                     FRETHKTDIEFYLWLQFVADQQLAECDAICRQQNMRIGMYRDLAVGVSPNGAETWNDK
                     QLYCLNASVGAPPDVLGPQGQNWGLTPMNPHVLKQQAYRPFIELVRANMSHCGALRLD
                     HIMSLLRLWWIPKGDSAVNGAYIRYPVDDLIAILALESQRHQCLIIGEDLGTVPKEIV
                     SKLKNAGILSYKIFYFEFDEQGKSRDLQHYPYQAMTTLSTHDLPTINGYWRGYDFELG
                     EKFGVYPNSDILAILKQDRVNAKAKILQRLQEHNVEIAPEISEQLNSPVNTAFIHCLQ
                     TYVANVSSGLFGFQPEDWLGMTEPVNIPGTSTQYANWRRRLSVNLEEIFTDKDIQALL
                     KEVNLIRKNQ"
     gene            complement(13872..15533)
                     /locus_tag="PWY43_000757"
     CDS             complement(13872..15533)
                     /locus_tag="PWY43_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDL2135656.1"
                     /translation="MSEIALTVSLLSLVAVLGLWIGHIKIRGVGLGIGGVLFGGLFVS
                     HFMAQFDVKLDAHTLHFIQEFGLILFVYSIGIQVGPGFFASLKHSGLKLNGFAVLIVL
                     ISGILVILIHKFFNVPLPVILGIFSGAVTNTPSLGAGQQVLAELSAESVTEIMGMSYA
                     IAYPFGIIGILLSMWLIRILFRINIEAEAEAFNKQQNRGKDQLSTLNVRLTNPNIHGL
                     ALKQIPDFNLHEVAYSRLKRGNELSIPRVDTILQIGDILHLVGEESSLRKMQIILGEA
                     VDVSVSTKGTIYRSERAVVTNEKVFGKQIRQLMLKGKYDVVISRLNRAGVELVPTGDM
                     TLQFGDVLNLVGRQADIEMVMTIIGNAHSKLQQVQMLPIFIGIGLGVLLGSVPIYLPG
                     FPVALKLGLAGGPLVVALILARIGSFSKLYWFMPPSANLALREIGIVLFLAVVGLKSG
                     GRFLETLLSSEGVEWICYGAIITFVPLMIAGLIARMYNKVNYLTICGLLAGSMTDPPA
                     LAFANEIKEGNGAAALSYATVYPLVMFLRIMLPQVLAIMLWVSGG"
     gene            15751..17127
                     /locus_tag="PWY43_000758"
     CDS             15751..17127
                     /locus_tag="PWY43_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDL2135657.1"
                     /translation="MLSNEVFISIIVLLILSLLRINVVIALIISALTCGLIGNLGVEE
                     AGFTEALEKTIKSFTGGLGGGAETAMNYAVLGAFAVALSKSGVTDLLAYKVIKSFGHS
                     PSGRSVTWFKYIVLFVLVAFAMSSQNLIPIHIAFIPILIPPLLSVMNKLQIDRRAVAC
                     ALTFGLTATYMLIPAGFGQIFIESILVKNINQAGEPFQLVTTTAEMTKAMLIPVSGMI
                     LGLLFAFFVSYRKPRVYIEDKPEPTSDDIEARVANVKPFHIGISILAIIATCSVQLAT
                     NSTIIGGLVGLAIFALGGVFRLKESNDVFQAGLRLMAMIGFVMIAASGFAGVVNSTGG
                     VPALVESLRDVISSKEMAALLMLVVGLFITMGIGSSFSTVPIITSIYVPLCVSFGFSP
                     LATMAIVGVAAALGDAGSPASDSTLGPTSGLNADGKHDHIWDSVVPTFIHFNLPLLVF
                     GWIAAMTL"
     gene            17228..18031
                     /gene="xthA"
                     /locus_tag="PWY43_000759"
     CDS             17228..18031
                     /gene="xthA"
                     /locus_tag="PWY43_000759"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL2135658.1"
                     /translation="MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPY
                     DLVNHLGYHVFHHGQKGHYGVALLTKKEPIAVRKGFPTDVLDAQKRMIMADIETDFGV
                     LTVLNGYFPQGENRSHETKFPAKQKFYADLQNYLESELKPENPIIIMGDMNISPTDLD
                     IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLVDTFRAGNPDVNDKFSWFDYRSK
                     GFDDNRGLRIDLILASKSLAERCVETGIDLDIRAMEKPSDHSPVWATFK"
     gene            18185..18769
                     /gene="lptC"
                     /locus_tag="PWY43_000760"
     CDS             18185..18769
                     /gene="lptC"
                     /locus_tag="PWY43_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDL2135659.1"
                     /translation="MNKRLTVILLIIVAVLGGWYYSLQEKENTGLDQLIKKEGQPEYV
                     GNKMSTTVYDLKGNPQYFAQATEIKRYESTERTEFFKPFIELFAKESTQKQWKVSADY
                     AEITKEKMLNLKGNIKLVALDPASRLQQITTDTLTVDLNTQDIFTESTVKSIGSGFTT
                     TGVGLKGNLKQQVATLQKDVKSYIEPTIIQETKE"
     gene            18793..19299
                     /gene="lptA"
                     /locus_tag="PWY43_000761"
     CDS             18793..19299
                     /gene="lptA"
                     /locus_tag="PWY43_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HDL2135660.1"
                     /translation="MKLMFKSILVSVLLGSSFSAYALKGDTDQPINIDSGSQSLDMTN
                     NIVTFSDNVVITQGSIKVTAANVKITRQEGKKETIDATGSPVTFQQTLDNGKPVNGKG
                     NSVHYDLNSEFLTLIGNAELKQQGSFIKANKITYDVKKQQLKATSGGKTRVKTVLIPN
                     ELQDNKKK"
     gene            19311..20036
                     /gene="lptB"
                     /locus_tag="PWY43_000762"
     CDS             19311..20036
                     /gene="lptB"
                     /locus_tag="PWY43_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005727816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDL2135661.1"
                     /translation="MSVLYAEHLAKSYKQRQVVKDVSLNVKSGEIVGLLGPNGAGKTT
                     TFYMVVGLVRHDHGTIRIDDQDISTLPMHDRAKQGIGYLPQEASIFRRLSVYDNLMSV
                     LQVRKNINDKQRKERAEELITEFHIEHIRNSLGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKKIIVNLKERGLGVLITDHNVRETLDVCERAYIVGSGEMIAS
                     GTPQQVLENPDVKRVYLGDQFKL"
     gene            20078..20590
                     /locus_tag="PWY43_000763"
     CDS             20078..20590
                     /locus_tag="PWY43_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2135662.1"
                     /translation="MTKLTEFLSPENVRQGVLISSKKRALELVGKIVAESINQTEQQS
                     EALCPIECFGNLFKREKLGTTGLNQGIALPHAKLPACGDIELTKPIAVFLQLEEAIDY
                     EAQDNKEVDLIYAMMFPENSCEQYKSCLPAIAQKLTDKSLLKQLRAAESAEDIWQILV
                     YADNHLKDEE"
     gene            20603..21466
                     /gene="rapZ"
                     /locus_tag="PWY43_000764"
     CDS             20603..21466
                     /gene="rapZ"
                     /locus_tag="PWY43_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL2135663.1"
                     /translation="MELIIISGRSGSGKSVALRALEDAGYYCVDNIPLPLIPTLTYYL
                     TQENRSAVISLDIRNLPNTPNAIEELLTQLEPLNAKLIFLDCERNTLIRRYSDSRRIH
                     PLSTQDDLSLEGALDLEQKLLEPLVQNANYIIDTSHLSSHELAENLRQILQGSTEKEL
                     KIIFQSFGFKYGLPADSDYVFDVRFLPNPHWNPELRPMTGLEQPVIDFLERQTEVHNF
                     IYQTRNYLEMWLPMLEQNNRSYLTIAIGCTGGKHRSVFIAEQLAKYFQAKGKNVQVRH
                     KSLEKHHKKTS"
     gene            21549..22733
                     /locus_tag="PWY43_000765"
     CDS             21549..22733
                     /locus_tag="PWY43_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL2135664.1"
                     /translation="MKSADIVIVGGGMVGLALAGLLKDVDCQIKIIEKNTPKFDADTI
                     YHRVSAINASSQKILEQIGAFQHIPAQRLSPYTEMKVWEKDSFANIHFDNNDNEIKQL
                     GLSQLGFILENAVIQHALWQQVSQQANVEYIIAAPQTLGVSENGAFLTLDNGEMLSAK
                     LVVGADGANSWVRNQMKMPLTSKDYQHTALVCNVKTTEKHGKTARQIFAEDSIFAFLP
                     MADEHLCSIVWSLPPNKANELKNASEAEFNKALTIAFDNQLGLCEVQSLREIYPLAAR
                     FCRDFAQPRIALIGDAAHTIHPLAGLGVNLGFADARTLANEIQKQLVNNQDIGEYRHL
                     RQFERERKAQAMKLLATMEGLKQLFDGNHPLKKLFRGIGLSLTNQLPFVKKQLIQQAI
                     YL"
     gene            22865..23449
                     /locus_tag="PWY43_000766"
     CDS             22865..23449
                     /locus_tag="PWY43_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein HlpB"
                     /protein_id="HDL2135665.1"
                     /translation="MNKLTKISATALVALFLAACDKPAEKAPATEPTKTTEAAPTTTA
                     PAAEAAKPAETAVAAPSEAAKADYQKLVDWNTAQESTMATAQAELQKKLATQDPKQIQ
                     EGLSAFNKKVEETVKSLDAIEVSDAQIKTFKEKTKSVLTLSSEVISEQVKTISTPNDQ
                     AALQAVQQKAQSLIEAGNELQKLNVELQQRFSAK"
     gene            23516..>24044
                     /locus_tag="PWY43_000767"
     CDS             23516..>24044
                     /locus_tag="PWY43_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein Hlp"
                     /protein_id="HDL2135666.1"
                     /translation="MNTLAKISLSVLFTLFLTACEQPNSTKTQSSAESPVQVKEESKE
                     EVKPADTGAQDYKMLREWQDTQEKALNDAIKAATETLTDKQKADSTLMQETVNNALLA
                     QIDHIKVSAETLNIQNNEVKALKDKTLEVLTLGSQMIVEGAKMEKNPTPEAHKAFGEL
                     QTKLNQLAEEGQQLEN"
BASE COUNT         7533 a         4909 c         4671 g         6931 t
ORIGIN      
        1 acctcttttc tgcttttgct tattggtttt atttaggctg aatagtcgcc tttttatcta
       61 aagaagaaat catcctaaaa tacttttttt tagggttaga gccataatta tttaaaaaac
      121 gccaattcag aaaaattaaa taaattttgt gatcaacctc tcatttttaa tgcctatttt
      181 tgtgaagcag atcacaaaat gataaaaaat gttacttaga tcatactttt agcttggggg
      241 gcgtagagca aagggtgatc tcaaatagat tgtatagccc ataatttgcc ataatttaat
      301 aaatgggttg tttcatatcc gtttctttta tttaacttca aggagttttt tatgaaaaac
      361 aaatgtgtca aattcacctt agctactctt gctaccttag ttctttcagg aacagtaatg
      421 gcaaaaatga cggaaggtaa actcgttatc tggattaacg gtgataaagg gtacaatggg
      481 cttgccgaag tcggtaagaa atttgaaaaa gataccggtg ttcaagtttt agttgaacac
      541 ccggatagac ttgaagaaaa atttgcacag gttgcctcaa ctggtgacgg tccggatatt
      601 atgttctggg ctcacgaccg ctttggtggt tatgcacaat ccggtttatt ggctgaagtg
      661 aatgtaagca aagaatttaa agataaattt gtcgattttg cttgggatgc tgaaacttac
      721 aacggcaaaa ttatcggtta tccggttgcg attgaagcga tttcattaat ttataataaa
      781 gatttagtga aagaagcacc aaaatcttgg gaagaaatta ttgagttaga taaaaaactc
      841 aaaaaagagg gcaaaaatgc cattatgtgg aacttatccg agccctattt cacgtggccg
      901 attgcggctt caaacggagc ttatgcattc aaatttgcta acggcaaata cgatccaaaa
      961 gatattggtg tgaacaatga aggggcagta aaagcactac aatttgtagt cgatatggtg
     1021 aaaaataaac atattagtgc ggatatggac tatgccgttg ccgaagcctc ctttaataaa
     1081 ggacaaagtg ccttaaccat caatggtcct tggtcttggg gcaatattga taaaagcggt
     1141 attaaatatg gtgtaacttt actcccaaca ttaaacggtc aagcttctaa accattcgta
     1201 ggggtgttaa gcgccggtgt aaacagctca agcccaaata aagatttagc caaagaattc
     1261 ttagagaatt atttactcac cgacgacggt ttagatgcag tcaataaaga taaaccttta
     1321 ggtgcggtgg cgttaaaatc ttaccaagag aaattagcgg ctgatcctcg tattgctgca
     1381 acgatggcaa atgcgaaaaa cggtgaaatt atgccaaata ttccgcaaat gtcttctttc
     1441 tggtatgccg aaaaatctgc tatcaataat gcagtgaccg gtcgtcaatc ggtgaaagaa
     1501 gcattagatg atgcacattc acgcattcaa aaacaacaat aattcttcat cattctgagt
     1561 gggttattat gacccactct atttttagat tcaccctaaa tacaataaaa gtacagtaaa
     1621 agtaccgtaa caaaatgcag taaaaattcg aggtgctgtt atgctaactc aaactcaatc
     1681 caaatccact cattggctca aatatctctt agccggatta gtcttgctcc ttgacttcta
     1741 tcttgttgtc ttaatgtact cacaaggtga atatcttttt gctattttaa cgctgattat
     1801 tctaacttcc ggtgtctata tttttaccaa taaaaatacc tatgcttggc ggtatgttta
     1861 ccctggcatt accggaatgg caatttttat tttgtttccg ctggttgcga ctattgcaat
     1921 tgcgtttacc aactatagtg gttctaatca attatccttt gaacgagcgg tatccgtctt
     1981 aactgaacaa cgctattttg ccggagataa atatcaattc accctttatc cgcaagcaga
     2041 taataaatat caaattgcat taaccaatcc gacgacagag caaacttttg tgtcagagcc
     2101 gatttcattg gctaccggaa cgaatgtggt tgtaaccgag caaaacagac caaccggcga
     2161 agtcgctccc cttaaagtga ttacgcaaaa tcgggccgca cttcaatcaa tgaaagtggt
     2221 tttaccgaat aataatgaac tgacaatgag ctcattacgt caattctccg agcaaaaacc
     2281 acgctatcaa tttgatgaag aaaataaaat attactcaat aatgagactg gcaaacgtta
     2341 tcaagcaaat gacgaaaccg gtttcttcca atcgattgat gaaaacggtc attggcaaag
     2401 cgaaacactt gaaccaggtt atactgtcac atcaggcttt aataacttca ttaaaatttt
     2461 taccgatgaa ggtattcaaa aaccttttgt ccaaatattt atttggacgg tgatcttctc
     2521 tttattaacc gttgtcttta ccgttattct cggtatggtt cttgcctgtt tggtacaatg
     2581 ggaggcatta aaaggtaaag cagtctatcg tgtcttattg attttacctt atgccgtacc
     2641 atcatttatt tcgattctga tttttaaagg actatttaac caaagttttg gtgagatcaa
     2701 tatgatttta aaccaactat tcggcatcag ccctgaatgg tttaacgatc cgctccttgc
     2761 caaagtgatg atcttaattg tcaatacgtg gttaggttat ccttatatga tgattttatg
     2821 tatggggtta ctcaaagcta ttccggctga tttatatgaa gcctccgcaa tggacggtgc
     2881 ttccacttgg caaaacttca gtaaaatcac tttcccatta ctgttaaaac cacttactcc
     2941 gttaatgatt gcttcgtttg catttaactt taataacttt gtattaattc aactattaac
     3001 aaacggtcgc ccggatatga ttggaacaac cactccagcc ggttataccg atttattagt
     3061 cagctacacc tatcgtattg cctttgaagg aagtggtacg caagatttcg gcttagcagc
     3121 cgccatcgca accattattt tcttattagt cggtggcttg gcactcttaa atatcaaagc
     3181 aaccaaaatg gaattataaa agcgggaggg atttaacaat cccgacccca aactaacttt
     3241 aggagttata tatggcaatt gttcaatcta aatctgtgcg ttatcgggta tgggcaaccc
     3301 atttcatctt aatctgtttc cttgccttaa ttattttccc tttattaatg gtgattggca
     3361 tttcgttacg accgggaaat ctggctatcg gtgatattat tccaagccaa atttcgtggg
     3421 aacattggca agccgctctt ggttttgatg ttacacacgc cgatggtaca gtcacactgc
     3481 cgccattccc ggtgctacgc tggttatgga actccatcaa agtggcaaca attacctcta
     3541 tcggcattgt aaccttatct acgacctgtg cttatgcatt tgcccgaatg aaatttaaag
     3601 gcaaaaaaac cattctacaa ggtatgctga ttttccaaat gttcccggca gtgctttcct
     3661 tagttgcctt atatgcgttg tttgatcgtc ttgggcagta tgtaccgttt cttggtttaa
     3721 acacacacgg tggtgttatt tttgcctact taggcggaat tgccttacac gtttggacaa
     3781 tcaaaggcta ctttgaaacg attgatggtt cattggaaga agccgcttcc cttgatggtg
     3841 caaccccgtg gcaagcgttc cgattaatct tattaccgct ttcagtgccg attttggcgg
     3901 tagttttcat tctttcattt attgccgcca ttaccgaagt accggttgcc tcactcctat
     3961 tgcgtgatgt aaacagctac accctagccg ttggtatgca acaatacctc tatccgcaaa
     4021 actatttatg gggtgacttc gcagcagcgg cggtcttatc cgcaattccg attacgctgg
     4081 tcttcttatt ggcacaacgc tggttaatcg gtggtttaac tgccggcggc gtgaaaggct
     4141 agtttgcaaa aaattctcta aaaacgaccg cttgtcgagc tgatactgcg gtcgtcaatc
     4201 tttattaggg ctgtctatga aaaatgcgtt accttatctc tgcttattct atacctcaag
     4261 cctttttgcc caatcttggc aacattctca tttttctgat tttaacgata atctcgaaaa
     4321 acggttgtat caatctcaag ttcaattgga aaaagggagc tatccgctta ggttcaaatt
     4381 tggcgaacaa tgttatcaac cccaacaagc gataaaatta aatcaatcgg ttgcgttagt
     4441 cccttgtata aatgaagctc cacaactgcg gttgtttcgt caaggcaact atctggcaca
     4501 aattgatctg cgaagcggca ctccgacact cacaatcagc gtagaacagc agctacagca
     4561 caatgaagcg atgtttcaaa gttgcccgaa ttgggataaa aaaccgattg aaattgatgt
     4621 ttcttccact tttgccgaag gcgaatcggt tagcgatttt tattcaggca acaccgctat
     4681 cgttaaaaac ggcaaagtca ccttaatgcc caatgaaaac gcaagcggtc tgattttgct
     4741 ggaaaaaagc aataccgaaa atacggcaac gtttgattgg aaaaatgcca ccgtctattt
     4801 tgtgctgacc gaccgctttt ataacggcga tccgaccaat gaccacagct accaccgcca
     4861 aaaagacgga atgcaagaaa tcggcacctt tcacggtggt gatttaaaag gattaaccga
     4921 aaagctcgat tacctacaac aacttggggt aaacgtattg tggattagct ctccgttaga
     4981 acaaatgcac ggctgggttg gcggcggtga taagggggat tttccgcatt atggctatca
     5041 cggttactat catttagatt ggacgaaatt agatgccaat atgggtactg aagacgattt
     5101 aagtcgcttt gttcaacaag cccaccagcg tggtattcgg gtgctatttg atgtggtgat
     5161 gaaccatacc ggctacgcga cgcttgccga tatgcaggag tttaactttg gcggatttta
     5221 cttaacaccg gacgaaatta gccgcacatt aggcgaaaaa tggacacact ggaagccgaa
     5281 atcaggacaa aattggcaca gtttcaatga tttcattcgc tttaacgata atcaggcttg
     5341 gcaaaattgg tggggaaaag aatgggttag agctgatatt gccgattacg acagccctaa
     5401 atttgatgat ttaaaaatgt cgctcgctgc ccttcctgat ctaaaaaccg agagcgaaca
     5461 agcggtcaaa ttacctgaat tttttgcaaa taaaaatacc aatgccaaaa cattaccaaa
     5521 cgccaaagtg cgtgattatt taattggttg gttatccgat tgggtcagaa aatatggcat
     5581 tgacggtttt cgggttgata ccgccaaaca cgttgaaaaa tccacgtggc tggtattaaa
     5641 acaatcggca caacaagcct taaacgaatg gcaaaaagcc aatccgaacg ccggttttaa
     5701 cgatcgcttt tggatgacag gagaagcgtg gggacacggc gtgttcaaaa gtgactatta
     5761 tcaaaacggt tttgatgcaa tgatcaattt cgattttcaa gatcaggcta aacaagggtt
     5821 aaattgcttt gcccatattg agccgattta tgccgaaatg aatcgcaaat taagcgactt
     5881 caatgtgttg agctatttat cttcacacga tactcgatta tttttccatt cggatagtga
     5941 acaaaatatc gaaaaacaca aaaccgccgc aaatttgctg ttgctttcac ccggtgcagt
     6001 gcaaatctat tatggtgatg aaagtgggcg gacatttggt gcgaccggtt cagacctgat
     6061 acaaggcacc cggtcggata tgaattggca agagttgcag caagatcctc agaaacaggc
     6121 attgcatcaa cattggcaaa aattgacaca attccgccaa cgccatccgg ctattggcgc
     6181 aggtgtccat caactcatca aaaatgatag ctactttgca tttaagcgtg tgttgaatga
     6241 agataaagtg atggtggttt gggccggaaa ttaaatctac tttggcttca agttttctct
     6301 cttctgggga aaacttgaag ccggttcggg gaaacaagtt gcaaaaaatt actaaaaata
     6361 gaccgcttgt tccccatgag cttgtgatca atgatacccc atcaacatca gcagttcttt
     6421 cgtatagctg attgcttcgt tacgattcgt cacaccgatt ttctgataaa gatttcggat
     6481 atgggtttta atcgtggttg cagccacttg caattcatct gaaatttgtt cattgctata
     6541 tccggagtaa atcaaaccca atacttgcca ttcccgctga gtgagcggac taatttttaa
     6601 gagttccggc actttcggat ttttcaataa ttgattcaca aagttttcat caaaatgggc
     6661 aaatttttgg cgataaaatt gattgatgtt gcgtaaaata aactgtgctt tgtgcaatac
     6721 cagttcatct agcacattaa gttgcaataa atggcggatc tgctgtgcca ttacatcccc
     6781 ttcaatcaca aacgcactga taaaattcgt ttggcgggtg agttttagtg cctgaattaa
     6841 atcttgctga gccaattctt tctcgccttg taaaaaataa agccggttac gaacaattaa
     6901 tgcgcgattt aaatcgctaa tcagagaaaa tttttcagcc gtttcaatta aattatctaa
     6961 aatggctttc gcctcgtgat attcctcaag caagattcga gaccgtgcaa tatttcgcca
     7021 ttgcacttgg gtaaaatgat ttttatctga aaccggtgag gggttttgaa ttaaccagtt
     7081 cctcaccgca gaaacgtcat tggtcatctg ccaataaaat atacgtacct gatcggcgtt
     7141 ggcaatccaa tcgtggtgat aagaatgaga atgttctaat tgttcgacct cgcctaataa
     7201 tcgggcagtg ttatccaaat tgcctcgagc caacgagatc ttcgtgagta aagttaagca
     7261 ttgtaactta tcttcaaatt tagataaggc attcattccg gcaactgcca ttgattcagc
     7321 tttatctaag ttataccatt cccataaaat cttgccttta gaacgtagca aaaattcata
     7381 catcggcact ttttgtagat ggttttcttt cacaaactcg cttgctttat ctaacatttc
     7441 ataggctgct tgtgaaaacc cttgggcaag caaaatttca gattgttgaa gctgtgacca
     7501 caaaatattg tggtaagtat gatgctgacg tgccatacgc tctgcttttt gcaacattgc
     7561 taatgcttct gccagattgc cgtggcaatg atgagcttcg ccaataatag acgttgcaac
     7621 aatatgagca taataggcat tttctgaaag atcgtttaac gcttcagacg ctaattgaag
     7681 tgcggtattc tcatcaccgg aattgatagc aacttgtgcc cgtagcacat taaattccgc
     7741 attggcagtt ttgctcaatt caattttatt ttcagttaat gcacgagaaa aatccgctaa
     7801 aataccacct acttcaatat ggcggtgctg gctttgtacc aaccaggctt tgagtaacac
     7861 caaattagta tgttcggtta aatgggcgta atctaagcaa tttaagctct cttccaataa
     7921 tttgagttct ccttgatgaa acaccgtcca agcgtgtttg ctcaaaatat tgagtagtaa
     7981 tccggtatcc gataactgca ttgcgtgatg taaggcttct gtaacatagc ctaattgtaa
     8041 ccacgcattc gccgcctttt gatgcagttg cggtaattca gcatataact cattttggca
     8101 acataaattt aaaaaggaag caaaaagcgg atggaatttc caccagctat catcactact
     8161 ctgccactta ctgtttgcca tttgttgtaa aaacaaacct tgcttttcga gagactctaa
     8221 tttggtgcga ctgttttgct cccctgtaac agcctgcact aaagactcat ttattgaatg
     8281 caaaaccgaa caacgcaaaa taaacaaacg agtctcttca tcgactttat taagcacttc
     8341 atcggctaaa tattcgttaa tatgaaaatg gtttgatttg gctaaacgtt tggcaatatc
     8401 ttgcagtggt acttgtagcg tgtgtgattt attttttgca aaaaggctaa ttagctgaag
     8461 ggctgtgggc cacccttcaa cttcgttaca cagtgcgata acatcttgct gatttaactc
     8521 cgaacctaat cttgcttgaa aaaaagcggt actttcttgg tgatcaaaca ttaactggtt
     8581 aatatcaatt tccaataatt gttcttgcac tcttaaactg gcaatactta acgggggaat
     8641 tgatcgagaa atcaagataa gcgtcatatt agacggttga tgtttaatcc aatattttaa
     8701 tgcttcgtga atttcatcat tttcaataat atgataatca tcaatagcaa gataaaaatg
     8761 ctctgaaaat gccgcggctt taatcaataa ttgattaaat aaagctaata aattagcttt
     8821 acgattttcc tctaacaata cgtctatttc ttcattgatt gctgaatgca atgccgcact
     8881 aaaataagtg gcaaaacgat ctgaattatt atcgccttca tctaacgcat accagccgat
     8941 attcttttta ttttcaatcc attgggaaac cagagtggtt ttgccataac ctgccggtgc
     9001 atttatcaat acgaccggat aactgcaaga tttgtctaat tcttgaatta aacgttttcg
     9061 aggaatgctg ttttgtaatc gaaaagaaca aacaagtttt gatggtatta acataataaa
     9121 ttcaattttc tctcataaca aaatagggat gatatatcca ccatttgtgt tctcactact
     9181 tccttataat cataccatac aagatgatta ttgggatgat ttgattttat ttggatttta
     9241 cataatagcg acattcatct ttaaccgggg gtttcctatg acatttaatt ctattgtaga
     9301 aaaatactgt cgctactttg atgtgacaga tccaaaacac tttaccttac agcagtggta
     9361 tcaagttgtt gctgaaggtt cccttgagct aatttatagc cagcctgcaa ccaaaccgac
     9421 agaatcacgc cacgtgaact acctctcaat ggagttctta atcggtcggt taaccggcaa
     9481 taatttaatg aatttaggtt attacgaaca aattcgtgac tatcttaaac aatatcaagt
     9541 tgaattagta gatgttttag aacaagagcg tgatccggca ctgggtaatg gcggattagg
     9601 tcgtcttgct gcctgctttt tagattcgat ggcaactgtc ggacaaaatg caaccggcta
     9661 tgggcttcat tatcaatatg gtctgtttaa acaaagcttc aaagagggaa tgcagaaaga
     9721 agcagcggat acttgggctc gtgacagcta cccgtggcat cgttttaatc cgtctaaaac
     9781 ccgccatatc gggtttggtg gaaaaatcaa acatatccaa gccgatcaat atgaatggca
     9841 gcctaaattg accattcaag gcaaagcctt tgacttgccg attgtaggtt ataaaaacaa
     9901 catcattcaa ccattacgtt tatggcaagc agatagtgac caatcttttg attttgatgc
     9961 ctttaatgag ggtaaatttt taagtgccga taaaacgatt gtcaatgcag ccgcactaac
    10021 tcaagtgctg tatccaaacg acaaccatca agccggacaa aaattacgct taatgcagca
    10081 atacttccat tgtgcctgct cggttgccga tattttagat cgtcacatta gcgaaggtta
    10141 ttcgttagaa gatttagcaa aacgccaagt tattcaactc aatgataccc atccaacgct
    10201 cgctatacct gaattgatgc gtgtactgtt agatgaacat caattcactt gggagcaggc
    10261 ttgggatatt tgcagcaata cctttgctta cactaaccat accttattac ctgaagcact
    10321 tgaacaatgg gatcaacgcc tatttaagca gctattacct cgccattatc aaattgtgga
    10381 aaaaatcaat gatattttcc acgacaaagt acaggcagaa tttggtgatg atgcaaaaat
    10441 ttgggaaaaa ttagccgtgc tatttgatta ccgtgtgcgt atggcaaacc tctgcgttgt
    10501 cacctgcttc cgtgtaaacg gcgttgcgga aattcactcc gatctcttag tgaccgatct
    10561 attcccggaa taccacaaat tattcccaac aaaattctgc aatgttacta acggcatcac
    10621 acctcgccgc tggattcgac aagctaatcc acaattaagt gatttattgg atcgtacctt
    10681 aaaacaagat tggacacaca atctggaatt attaaaaggc gttgaacaat atgcggatga
    10741 caccggtttc cgtgaagaat atcaacgcat taaacgcaat aacaaagtgg ttttagccaa
    10801 tgaaatccat aaaacattag ggttaaatgt caatcctaat gcgatttttg atgtgcaaat
    10861 taagcgtttc cacgaatata aacgccaaca tttgaatttg ttaaacatta ttgcgaccta
    10921 tcaatcgcta aaagctaacc cgaaccaaga ctacacacca cgtctattcc tattctcggg
    10981 caaagctgca ccgggttatt atttagcaaa aaatattatt cacgctatta ataatgtggc
    11041 agatattatt aataacgaca agcaggtaaa tgatcgacta caagtggcat tcttaccgga
    11101 ttatcgagtc agtcttgcgg aaaaaattat tccagcagct gatgtatcag aacagatttc
    11161 aatggcaggt aaagaagcct ccggtaccgg taatatgaaa ttggcactca acggcgcatt
    11221 aaccttaggt acgctagacg gtgccaatgt tgaaattgct gatatggtcg gtgaggagaa
    11281 tgtctttatc ttcggtcaca ccgtagaaag cgtgagggaa ttattagcaa aaggctacca
    11341 accgaaagcc tactataacc aagatccgat tctgaaaaat gcggttgatt tcttagccac
    11401 aggaaaagcc tctaacggag ataaagaggc attcaagctg atgttagata gcctgttgga
    11461 acgagatcct ttcctagtct ttgccgactt tgatagctat cgccaagcac aagagaaaat
    11521 tggtgtagcc tatttagatc aagaaacttg gctgcgtagt gcaattttaa atactgctcg
    11581 tttgggtgca tttagctcag accgctcaat taaagattat caacaacgta tttggttgaa
    11641 tagataataa attcgggcga cttctacaat aaagaaagtc gcctattttc tgctgtattt
    11701 catatgtatt tatatatgga gagaaatgat gaaaataact gccaaacaat attatcgggc
    11761 tggaattatg ccctattttt tcgatgagcg tggtgtaaaa aaaacagcat ctgaaaaaat
    11821 aaaacactcg ttattcaaag cctttaacgg caatactcaa gcaagagaaa cacctatccc
    11881 tacggtcaac attttttacc agcaccaacc ctgtttttta ccttttcatt tagcggataa
    11941 aaaacatccg ttaaccggta cttggcagct acaactagaa aattcaaacc aaattatttc
    12001 cggcaaaatt aaagcaaggg gaattacatt acctcaagat ttgcccttag gttatcacca
    12061 attacagcta aaaacggtag ataaaacctt taattgcacc attattatcg ctccggaacg
    12121 ctgctaccaa ccacaagccg tcattgaaca aaaaagattg tggggaacct tcctacaact
    12181 atacacctta aaatcagccc acaactgggg gattggcgat tttggcgatt taaaagactt
    12241 cattcaacag attgcccctt accaagcaga ttttatcggc ttgaacccga ttcactcact
    12301 ttttccggca aacccgaatt ctgccagccc atacagtcca tcctctcgcc gatggctgaa
    12361 tattgcttac attgatatca atcagctagc ggaatttcaa caaagtgatg aagcacaaac
    12421 ttggtttcat tctgctgaaa tccaacaaca attatcagaa ttacgcactc aggattggtt
    12481 gaattacgag caaatcattc cgttgaaatt gaaagggcta cgctttgctt tcgcaaaatt
    12541 tcagcaaaat atgaccgctt gcgctgcccc tgcttttatc gaatttgtac aaaaaggtgg
    12601 tgaaagctta caagttcaag ccacatttga tgcccttcat cagcatctat cggcacaatt
    12661 tgccgaccaa tggggctggg atttctggga gaaagcctac caagactacc actccgcagc
    12721 cgtgcagcaa tttagagaaa cgcataaaac cgacatagaa ttttaccttt ggttacaatt
    12781 cgttgccgat caacagctag cagaatgtga tgctatctgc cgtcagcaaa atatgcgtat
    12841 tggtatgtat cgcgatctcg ccgttggcgt cagcccgaat ggtgcagaaa cgtggaatga
    12901 caaacaactt tattgcttaa atgcctctgt cggcgctccg cctgatgtat taggtccgca
    12961 aggacaaaac tggggcttaa ccccaatgaa tccgcatgta ttaaagcaac aagcctaccg
    13021 tccgtttatt gagttagtca gagctaatat gagtcattgc ggtgcattac gccttgacca
    13081 tattatgtct ttactccgtt tatggtggat ccccaaaggc gatagtgcgg ttaatggtgc
    13141 ttatattcgt tatccagttg atgatttaat cgcaattttg gcacttgaaa gccaacgcca
    13201 ccaatgccta attatcggtg aagatttagg tactgtgcca aaagaaattg tgagcaagct
    13261 caaaaatgcg ggaattttat cttacaaaat tttctatttt gaatttgatg aacaagggaa
    13321 gagtcgagac ttacagcatt acccttacca agcaatgacc acattaagca cccacgattt
    13381 accgactatc aatggttatt ggcgtggcta tgattttgaa ttaggcgaaa aattcggcgt
    13441 ctatcctaat tcggatattt tggcaatcct taagcaagat cgagtcaatg ccaaagccaa
    13501 aatcttacaa cgcttacaag aacataacgt agagatcgcc cctgaaatta gcgagcaact
    13561 taattctccg gtgaataccg catttattca ctgtttacaa acctatgttg caaatgtaag
    13621 cagcggatta ttcggcttcc aacccgaaga ctggttgggt atgactgagc cggttaacat
    13681 tccgggaacc agtacacaat atgcaaactg gcgacgtcgg cttagtgtta acctcgagga
    13741 gatcttcaca gataaagata ttcaagcctt attaaaagaa gttaatttaa tccgtaaaaa
    13801 tcaataaaag ttagataaga aaaaaggcga ctttccgtcg ccttttgggc ttatatgctt
    13861 gatgtctatc tttatccacc tgaaacccac aacataatcg ctagcacttg aggtagcata
    13921 atacgcaaaa acattactag tgggtaaaca gttgcatagg ataacgctgc agcgccattt
    13981 ccctctttaa tttcattggc aaaagcaagc gctggaggat cagtcattga gccggcaagt
    14041 aatccacaaa tcgttaaata gtttactttg ttatacattc tggcaatcaa tcctgctatc
    14101 atcaatggaa caaaagtaat aatcgcccca tagcatatcc actcaacgcc ctcactgctt
    14161 aatagcgttt cgagaaatct accgccagat tttaaaccaa ctaccgctaa aaacagtaca
    14221 ataccgattt cacgcaatgc aagattggca cttggtggca taaaccagta gagtttactg
    14281 aaactaccaa ttcttgcaag aataagagca accaccagtg gtccaccagc caagcccagc
    14341 tttaacgcaa cagggaaacc cggtaaataa atcggtacag aaccaagtaa tactcccaaa
    14401 ccgatcccga taaatattgg caacatttgg acttgttgca actttgagtg cgcattgccg
    14461 ataatcgtca ttaccatttc aatatcagct tgccgcccta ctaaattgag aacatcacca
    14521 aattgtaacg tcatatcacc tgttggtact aattctacac cggctcgatt taaacgagaa
    14581 atgacaacat cgtatttgcc tttaagcatt agctgacgaa tttgtttccc gaagactttt
    14641 tcattcgtca ctacagcacg ttcagaacgg taaatcgtgc ctttggttga gacagataca
    14701 tctactgcct cacctaaaat aatctgcatt tttcgtaatg aggattcttc acctactaag
    14761 tgtaaaatat ctcctatctg tagaatcgta tctacacgtg gaatagaaag ctcattcccc
    14821 cgttttaagc gtgaataagc tacttcgtgc aaattaaaat caggaatttg tttcaacgct
    14881 aaaccgtgaa tatttggatt agtcagacgg acatttaagg tactaagttg atctttacct
    14941 ctattttgct gtttgttaaa agcctctgct tccgcctcaa tattgattcg aaataaaatc
    15001 cgaatcagcc acattgaaag tagaattccg ataatgccga acgggtaagc aatcgcataa
    15061 ctcattccca taatttcagt tacactctca gcgcttaact cagccaatac ctgttgacct
    15121 gctcctaaag aaggggtatt ggttacagcc ccggagaaaa tgcctaaaat cacaggcaaa
    15181 ggaacattga agaatttatg aataagaata actaaaatac cgctaatcag tacaatcagt
    15241 accgcaaaac cattgagttt taagccagaa tgttttagtg aggcaaagaa tccaggtcct
    15301 acttgaattc caattgaata aacaaataaa atcagcccga actcttgaat aaaatgaagc
    15361 gtatgggcat cgagcttcac atcgaattgc gccataaaat gggagacaaa cagcccacca
    15421 aataacactc cccctatacc tagccccacg cctctgattt taatatggcc aatccataag
    15481 cctagaacgg ccactaaaga taataaactg acagtaagtg caatttcgct cattacagcc
    15541 tctctttaat atgaaatacc tcaaaaatgg tatccaattc taccaaatct caaaaacgaa
    15601 aaatgagatc tatgtcaaaa aaataaaata ttgatagtat tactccaaga tgaacttcaa
    15661 aaaagttcct gaatttaaaa aaaattctcg acaatacttt tttttacagt aaattaaaca
    15721 attatttaaa taacctaagg atcaaacaca atgttatcaa acgaagtctt tatttcgatt
    15781 atcgtgctgt taatcctaag cttgcttaga attaatgtag taatcgcact tattatttct
    15841 gcccttactt gcggtctaat cggtaatctt ggtgtagaag aggctggatt taccgaagca
    15901 ttggaaaaaa caataaaaag ttttactggt ggtttaggtg ggggtgcgga aactgctatg
    15961 aactatgccg ttcttggtgc gtttgccgta gctctatcaa aatcaggcgt gaccgattta
    16021 ttagcctata aagtgatcaa atcctttggt cacagcccgt ctggtcgctc tgtcacttgg
    16081 tttaaataca tcgtattatt tgtgttagtg gcgtttgcga tgtcatcaca aaacttaatc
    16141 ccgattcata ttgcctttat tcctatttta attccgccac tattaagcgt aatgaataaa
    16201 ttgcaaattg accgccgtgc agtagcctgt gctttaacct ttggtttaac tgctacttat
    16261 atgttaattc cggcgggttt cgggcagatc tttattgaaa gcatcttagt taaaaatatc
    16321 aaccaagcag gtgaaccgtt ccaattagtg actaccacag cggaaatgac caaagcgatg
    16381 ttaatccctg tatcaggtat gatcttaggt ctattatttg ctttctttgt gtcgtatcgc
    16441 aaacctcgtg tctatattga agataaacct gagccaacct cagatgatat tgaagctcgt
    16501 gtcgcaaatg tcaaaccatt ccatatcggc attagtattt tagccattat tgcaacctgt
    16561 tcagtgcaat tggcaacaaa ttcgaccatt attggtggtt tagtcgggct agctattttt
    16621 gccttaggtg gtgtgtttag attgaaagaa agtaacgatg tcttccaagc tggtttacgt
    16681 ttaatggcga tgattggttt tgtaatgatt gctgcttctg gctttgccgg tgtcgtcaat
    16741 tcaaccggtg gcgtgccagc gttggttgaa agcctgcgtg atgtgatttc atcaaaagaa
    16801 atggctgcat tgttaatgtt agtcgttggt ttatttatta cgatggggat cggctcttca
    16861 ttctccacag tgccgatcat tacctcaatt tatgtaccac tttgtgtttc atttggtttt
    16921 tcgccactag caacaatggc gattgtcggt gttgctgctg cacttggtga tgcaggttct
    16981 ccggcatcag attccacact aggtccaacc tccggtttga atgcagatgg taaacacgat
    17041 cacatttggg attctgtagt accaaccttt atccacttta atcttccatt attagtgttc
    17101 ggttggattg cagcaatgac actttaagct ataatatgca aaaattttgc taaaaatgac
    17161 cgcttgtagc cattacaagc ggtctatttt cttctttatt ttgcaaaccc tacgattagg
    17221 attatcaatg aaatttattt cttttaatat caacggatta cgagccagac cacatcaact
    17281 ggaagctatt atcgaaaaac accagcctga tgtactcggt ttacaagaaa tcaaagtcgc
    17341 tgatgaagac ttcccttacg atttagtcaa tcacttgggc taccacgttt tccaccacgg
    17401 gcagaaaggt cattacggcg tggcattact gactaaaaaa gagcctatag cggtgcgtaa
    17461 aggcttcccg acagatgtgc ttgatgctca aaaacgaatg attatggcgg atatcgagac
    17521 cgatttcggc gtgctaaccg tattaaacgg ctatttccca cagggggaaa atcgcagcca
    17581 cgaaaccaaa ttcccggcaa aacaaaaatt ctatgctgat ttgcagaact acttggaaag
    17641 cgaattaaag ccggaaaatc cgattattat tatgggcgat atgaacatca gcccgactga
    17701 tttggatatc ggcatcggcg agccaaaccg caaacgctgg ctgcgtgacg gtaaatgttc
    17761 attcttaccc gaagagcgtg aatggctaga tcgcttatac agctacggct tggttgatac
    17821 tttccgtgca ggcaacccag acgtgaacga taagttctct tggtttgact accgttcaaa
    17881 aggttttgat gataatcgtg gcttgcgtat tgatttaatt cttgcttcaa aatcacttgc
    17941 tgagcgttgc gtggaaacag gcattgattt agacattcga gcaatggaaa aaccgtctga
    18001 ccactcccct gtttgggcaa cctttaaata aagttgcaaa aaaatctgag ttttcaaccg
    18061 cttgtcagtt tttagcccta caagcggtta ttttttctca cttttttgca ctttttacgc
    18121 tgtactatat ccctattcaa atctgtgcta caatcaaaca ctatttttag attatttttg
    18181 gattatgaac aaacgtttaa ccgttatttt actgattatt gttgccgtac ttggtggctg
    18241 gtattacagc ttacaagaga aagagaatac ggggcttgat cagcttatta aaaaggaagg
    18301 acagcctgaa tatgtgggga ataaaatgtc caccacggtc tatgatctaa aaggaaaccc
    18361 acaatatttc gctcaagcta ctgaaattaa acgctatgag tctactgagc gtactgagtt
    18421 ttttaaacct tttattgagc tttttgccaa agagtcgacg caaaaacagt ggaaagtcag
    18481 tgccgattat gcagaaatca ccaaagaaaa aatgttaaat ttaaaaggta atatcaaact
    18541 tgttgcatta gatcctgcat cacgtttgca acaaatcact accgatacgt taactgttga
    18601 tttaaataca caggatattt ttaccgaaag tacggtcaaa tcgataggtt ccggttttac
    18661 tacaacgggg gttggattaa aaggtaactt aaaacaacaa gttgcgactt tgcaaaaaga
    18721 tgtaaaatct tacattgaac cgaccattat tcaagaaacc aaagaataat ttgcttggaa
    18781 caaggaataa caatgaaatt aatgtttaaa tctattttag tgagcgtgtt actgggtagc
    18841 agcttttccg cttatgctct aaaaggcgat accgatcagc cgattaatat tgattcaggt
    18901 agccaatcgt tagatatgac taacaatatc gtgaccttta gcgataatgt cgttatcact
    18961 caagggtcga ttaaagtgac cgcagcaaac gtcaaaatta cccgtcaaga aggtaaaaaa
    19021 gaaactattg acgctacagg ctcacctgta acattccaac aaaccttaga taacggtaaa
    19081 ccggtaaacg gtaaaggcaa cagcgttcat tatgatttaa actctgaatt tttaacgctg
    19141 attggcaatg cggaactcaa acaacaaggt agctttatta aagccaataa aattacctat
    19201 gatgtgaaaa aacaacaact taaagctacc agtggcggta aaacccgagt gaaaaccgtg
    19261 ctaattccaa atgaacttca agataacaag aaaaaataag gttaaatgat atgtctgtgc
    19321 tttatgccga acatttagca aaaagctata aacaacgcca agtggtaaaa gacgtcagcc
    19381 tgaatgtcaa atcaggtgaa atcgtgggct tgctcggtcc gaacggcgca ggtaaaacca
    19441 ccactttcta tatggttgtg ggtttggttc gccacgacca cggcacaatt cgtattgacg
    19501 accaagatat cagcacacta ccaatgcacg acagagcaaa acaagggatt ggctatctac
    19561 cgcaagaagc atctattttc cgccgtttaa gtgtgtacga taacttaatg tcggtattac
    19621 aagtgcgtaa aaatatcaat gataaacagc gtaaagagcg tgccgaagag ctgattacag
    19681 aattccatat tgagcatatt cgcaatagtt taggtcaatc actttcaggt ggtgaacgcc
    19741 gccgtgtgga aattgcccga gcattagcag cgaacccgaa atttattctg ttagatgaac
    19801 cttttgctgg ggttgaccca atctcggtaa ttgatattaa aaaaattatc gtcaatttaa
    19861 aagagcgtgg tttaggggta ttgattaccg accataacgt acgtgaaact ctagatgtat
    19921 gtgaaagagc ctacattgtc ggtagcggcg aaatgattgc cagcggcaca ccacagcaag
    19981 tgcttgaaaa ccctgatgta aaacgtgttt atttaggcga tcaattcaaa ctctaaaaaa
    20041 tcccctcttt ggggatttca tctttagatg aaaaattatg acaaaactaa ccgaatttct
    20101 aagccctgaa aatgttcgcc aaggtgtact gatttcaagt aaaaaacgag cgttagaact
    20161 cgtgggtaaa atcgtggctg aatcaatcaa ccaaaccgaa cagcaatctg aagcactctg
    20221 ccctattgaa tgctttggca atttatttaa acgtgaaaag ttaggcacaa ccggcttaaa
    20281 tcaaggcatt gcacttcctc atgcaaaatt gcccgcttgc ggtgatattg agctgactaa
    20341 accgattgcg gtctttttac agctggaaga agcgattgac tacgaagccc aagacaacaa
    20401 agaagtggat ttaatttatg caatgatgtt tccggaaaat agctgcgaac agtataaaag
    20461 ttgcctacct gcaattgcac agaaacttac cgataaatcg ttattaaaac agctccgagc
    20521 agctgaatcc gcagaagata tttggcaaat tttggtgtat gctgataatc acctaaaaga
    20581 tgaagaataa tactgaggaa atatggaatt aatcattata agcggacgct caggctctgg
    20641 taaatcggta gcattaagag cattagaaga tgcaggttat tactgcgtgg ataatatccc
    20701 tttaccttta attccaacct taacctacta ccttactcaa gaaaatcgtt cggcagtcat
    20761 cagcttagat attcgtaatt taccgaatac gcctaatgcg attgaagaat tgcttaccca
    20821 gcttgagcct ttaaatgcta agctgatttt tttagattgc gaacgcaata ccttaatccg
    20881 ccgttatagc gattcccgcc gaatccaccc gctttccacc caagacgatc tctcgttaga
    20941 aggtgcattg gatttggaac aaaaattgct tgagcctttg gtgcaaaatg caaattatat
    21001 tattgacacc agtcatcttt cttcgcacga attagcggaa aatcttcgcc aaatactgca
    21061 aggctcaacc gaaaaagagc ttaaaattat tttccaatca ttcggtttta aatacggctt
    21121 acctgccgat tccgattacg tttttgatgt tcgcttttta ccgaacccac attggaatcc
    21181 ggagcttcgc ccaatgacag gcttagagca accggtgatt gatttcttag aacgccaaac
    21241 cgaagtgcat aattttattt accaaacccg caattattta gaaatgtggc tgccgatgtt
    21301 ggaacaaaat aaccgtagct acctgactat tgcgattggt tgcactggtg gaaaacatcg
    21361 ttccgtattt attgccgagc aactggcaaa atatttccaa gcaaaaggca aaaatgtgca
    21421 agttcggcat aaaagtttgg aaaaacacca taaaaaaaca tcttaaataa gtaggggctg
    21481 gtcctgtgcc agccctcatt tcaactattt tagggcgggc acagggcacc gcccctacat
    21541 ttgattttat gaaatccgca gatattgtta ttgttggcgg cggtatggtt gggctggctc
    21601 ttgccggact gctcaaagat gtcgattgcc aaattaaaat tattgagaaa aacacgccca
    21661 aatttgatgc cgatacgatt taccaccgtg taagtgcgat taatgcttca agccaaaaaa
    21721 tactggaaca gattggtgct tttcaacaca ttccggctca acgcctttct ccttacaccg
    21781 aaatgaaagt gtgggaaaaa gacagctttg ccaatatcca ttttgataat aatgataatg
    21841 aaatcaagca attaggactt tcacaacttg gctttatttt ggaaaatgcg gtgattcaac
    21901 acgcattgtg gcaacaggtt tctcaacaag ctaatgtaga atatattatc gccgcccccc
    21961 aaactctggg cgtgagcgaa aacggggcat ttttaacctt agataacggc gaaatgctgt
    22021 ctgctaaatt ggttgtcggg gcagacggtg caaattcgtg ggtgcgtaac cagatgaaaa
    22081 tgccgcttac cagtaaagat tatcaacata ccgccttagt ctgtaatgtc aaaacaaccg
    22141 aaaagcacgg caaaaccgct cgtcaaatct ttgctgaaga tagcattttt gcttttctgc
    22201 caatggcaga tgagcatctc tgctcgattg tttggtcgct gccaccaaac aaagccaatg
    22261 aattaaaaaa tgcaagcgaa gccgaattta ataaagcctt aacgattgcc ttcgataatc
    22321 agcttggctt atgtgaagtg caaagtttgc gtgaaattta cccgcttgca gcacgcttct
    22381 gccgtgactt tgcccaacct cgtattgcgt tgattggtga tgccgctcat accattcacc
    22441 cattggcggg acttggtgtc aatttaggct ttgccgatgc cagaacgctg gcaaacgaaa
    22501 tccaaaaaca gttggtaaac aaccaagata ttggtgaata ccgccattta cgccaatttg
    22561 aacgtgagcg taaagcccaa gcgatgaaat tattagccac aatggaagga ttaaaacagc
    22621 tatttgacgg caatcacccg cttaaaaaat tattcagagg aattggttta agcctaacca
    22681 accagctgcc ttttgtgaaa aagcagctta ttcagcaagc tatttactta taaaatgtaa
    22741 attttttcga aataacgtaa agatgaacga ttttatgaac taatacggag attacttgtc
    22801 tatttattgg tacaataacc agcctttatt ggcttttatt tttatattct tgaggaattt
    22861 tttaatgaac aaattaacaa aaatcagtgc aaccgcttta gtcgcattat tcttagcagc
    22921 ttgtgataaa ccagcagaaa aagcaccggc gacagaacct acaaaaacaa ctgaagctgc
    22981 accaactaca acagctcctg ctgcagaagc agccaaacct gctgaaacag ctgtagccgc
    23041 tccaagcgaa gctgcaaaag cagattacca aaaattagtt gattggaata cggcacaaga
    23101 aagcacaatg gcaacagcac aagcagaact acaaaagaaa ttagctacac aagatcctaa
    23161 acaaattcaa gaaggattaa gtgcatttaa taaaaaagta gaagaaactg taaaaagctt
    23221 agatgcgatt gaagtatctg atgcacaaat caaaacattt aaagaaaaaa caaaatcagt
    23281 tttaacactt tcaagtgaag tgatttctga acaagtaaaa acgatctcta caccaaatga
    23341 ccaagcagca ttacaagccg tacaacaaaa agctcagtct ttaattgaag ctggtaatga
    23401 gttacaaaaa ttaaacgtag aattacagca aagattctca gctaaataat ttaccattca
    23461 atgaaaagct agagtatttt gctctagctt ttttttgcaa ttactaagga ttactatgaa
    23521 cacgctcgct aaaattagcc tatctgtatt atttaccctt tttttaaccg cttgcgaaca
    23581 acctaattct accaaaacac agagctcagc agaatcgcca gttcaagtga aagaagagtc
    23641 aaaagaagaa gttaaacctg ctgataccgg cgctcaagat tataaaatgt tacgggaatg
    23701 gcaagacact caagaaaaag ccttaaatga tgcaattaaa gcggcaacag aaacacttac
    23761 tgacaaacaa aaagccgatt ctaccttaat gcaagagaca gttaataacg cattattagc
    23821 tcaaattgat catattaagg taagtgctga aaccctgaat attcaaaata atgaagtcaa
    23881 agcactaaaa gacaaaacct tagaagtcct gactttaggg tctcagatga tcgtagaagg
    23941 tgctaaaatg gagaaaaatc ctacaccgga agcacataaa gcatttggcg agttacaaac
    24001 aaaattgaat caattagctg aagaagggca acagttagaa aata
//
LOCUS       DANKBV010000025        23742 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.025, whole genome shotgun sequence.
ACCESSION   DANKBV010000025 DANKBV010000000
VERSION     DANKBV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23742
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.025"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(497..1507)
                     /gene="iolG"
                     /locus_tag="PWY43_000768"
     CDS             complement(497..1507)
                     /gene="iolG"
                     /locus_tag="PWY43_000768"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL2135667.1"
                     /translation="MLKVGIIGAGRIGRVHSESISKYVKGAEIKAISDVKITEELTAW
                     AKSMGIPEVYDDYKKILQDPEIDAVLVCSSTNTHAPISIEAAQAGKHVFCEKPVDADP
                     AKIREVLSAVEKAGVKFQVGFNRRFDHNFKAIKDRVAAGDIGEPHVIRVTSRDPEAPP
                     IEYVKVSGGMFFDMTIHDFDMIRYLSGSEVEEVFAVGTVLVNPEIGKAGDIDTAVITL
                     KLKNGAIGVIDNSRKAAYGYDQRAEVFGSKGAIHITNDTGSTAVFSGENGVVAEKPKY
                     FFLERYMQSFADEISCFVDSVVNDKPTLVNGNDGLQPVLIALAAKKSLEENRPVKLSE
                     VA"
     gene            complement(1509..2405)
                     /gene="iolE"
                     /locus_tag="PWY43_000769"
     CDS             complement(1509..2405)
                     /gene="iolE"
                     /locus_tag="PWY43_000769"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011817366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL2135668.1"
                     /translation="MKAENIQLGIAPIGWTNDDLPELGAENTFEQCVSEMALAGYTGC
                     EVGNKYPRDVAVLKHKLDVRGIQICNAWFSTFFVDGKREETIAEFIKHRDFLHAMGAK
                     VIGCSEQSRSIQGTTKSVFKEKPIFNDEDWKRMAEGYNELAKLAAEKGMKVCLHHHMG
                     TGIQTPEEVDRYMAEVNDDVYLLFDSGHLYYSEGCQKAMLGVLEKYIDRIIHVHLKDV
                     RDEVVAEVKAKDLSFLEGVKKGTFTVPGDGVIDFRPIFDILEKHDYKGWMVVEAEQDP
                     ALANPFEYALKGRKYIRETAGV"
     gene            complement(2532..4466)
                     /gene="iolD"
                     /locus_tag="PWY43_000770"
     CDS             complement(2532..4466)
                     /gene="iolD"
                     /locus_tag="PWY43_000770"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL2135669.1"
                     /translation="MQTTRLTVAQALVQFLDNQYLDVDGKEIKFVHGVAAIFGHGNVL
                     GIGQALAQNAGGLTLYQGRNEQGMAHLAMGFAKQNLRQRIIACTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDVFATRQPDPVLQQIEQPYDLSISTNDAFRAVSKYWDRVQRPE
                     QLMSACINAFRVLTDPAETGAVTIALPQDVQAESFDFPNSFLQKRVHRLERTPPTTAM
                     IKQAVTLIQTKKKPLIVCGGGVRYSQAGQILQKFAKQFGIPFAETQAGKSAVVSDHAL
                     NVGGVGETGCLAANRLAKEADLVIGIGTRYTDFTTSSKWIFQNPNVQFLNINVARFDA
                     YKLDGVQLVADAKTALEALSQAIPTDYQAQWENEISQAKADFADEIQRLRAVQYHPDF
                     TPEINDNLDHKAVHNEFIQLTGSQLTQTQVLGIVNETIDNNAIIVAAAGSLPGDLQRT
                     WLSKGEETYHLEYGYSCMGYEVSAAVGVKIARPEQEVYALLGDGSYLMAHTELVTSIQ
                     EGKKINVVLFDNMANGCINNLQIGNGMDSFGTEFRFRNGETNQLNGGLVPIDFAKNAE
                     AYGCKTYRVTNEAELRAALDDAKKQTVSTLIDIKVLPKTMAHGYGCWWHVGVAEVSES
                     ERIQQARQRSEEQLAKARKY"
     gene            4734..6650
                     /gene="iolC"
                     /locus_tag="PWY43_000771"
     CDS             4734..6650
                     /gene="iolC"
                     /locus_tag="PWY43_000771"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL2135670.1"
                     /translation="MATVNHQDRPLDLICLGRVAVDFYGQQIGSRLEEMTTFSKYLGG
                     SSGNVAYGTAVQGLRSSMLARVGDEHMGRFLREELQSVGVDTSHLITDKERLTALVIL
                     GIKDKETFPLIFYRDNCADMAITKDDFSEAYIASSRCLAITGTHLSNPKTREAVLTAL
                     EYAGRNEVKRALDIDYRPVLWGLTSLGDGETRYIDSAEVTKSLQEVLHHFDLIVGTEE
                     EFHIAGGSTDTLTALKNVRKVSNAVLVCKRGALGCSVFEGELPNDLDGGLNVYGVRVE
                     VLNVLGAGDAFMSGLLRGYLNNEGWEQACHYANACGALVVSRHGCSPAMPTKRELDDY
                     LTRAESVPRPDLDERLNHLHRVTTRKKQWENLCIFAFDHRKQLVDMANKVGADLARIP
                     KLKQLLLKAMEQTATQSGLPQNQAGILADTTFGQDVLNEITGKDYWIARPIEEPSSRP
                     LELEYGDLGSQLASFPRDHVIKCLAFYHPNDESGLKARQDRKLKEVYQACCRTGHELL
                     LEIILPADMPQSEAFYNQAIAHFYQLGIKPEWWKLPDVSRAQWQAISATIEANDPDCR
                     GILILGLDAPLEVFKDTFEQAKGCEKVKGFAVGRTIFGEPSEKWLAGELDDNTLISAV
                     SEKYRTLIDLWQQR"
     gene            6939..8279
                     /locus_tag="PWY43_000772"
     CDS             6939..8279
                     /locus_tag="PWY43_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HDL2135671.1"
                     /translation="MSCNTSPKRFHASWESIKQHQVPEWYEDAKFGIFIHWGIYSVPA
                     FAPPTCQLGEIDIDEQWFCNNPYAEWYFNSINVKKGPTYEHHIKTYGESFAYNDFLPL
                     WKAEKWQPAEWAKLFAKAGAKYVVLTTKHHDGFCLYPSKYTDFNCMQAGPKRDLMGEL
                     TENVREQGLKMGAYYSGIIDWTYSSAPIFTESQNFSNACPTYEYADYAYKQVVELIDR
                     YKPDVLWNDIGWPKVGEHMLPHLFAHYYNSVQEGVVDDRWNKLWCDFTSKEYKHGVAS
                     RDKKWEMCRGMGLSFGYNQVEDESHLISVKDLISLLVETVADNGNLLLNIGPKADGTI
                     PQEQVERLLALGSWLEINGEGIYKSRCSQHQTEQIGDVTYRYTKVGADLYLYIDGLTE
                     AQISLPLSVYGELQALNTDLKFTIENTAQGRVLHIQNYQPDWYVLGFKIADGEK"
     gene            8282..9622
                     /locus_tag="PWY43_000773"
     CDS             8282..9622
                     /locus_tag="PWY43_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2135672.1"
                     /translation="MSVSQRFINFMNTHVAPVARRMENQPHISAIRDGFIVVLPFLIV
                     GSFIMILLIPPFDENTQNAFGQAWWRFANWASPYGWNFFQMSFNAISLFTSASIAYNL
                     AKAYKREPLPAAFLSVMAFLLVAAPVKDGMMDIKFFGGIGLFSAIFIAIYSVEMTRLL
                     EFLKIKIRLPKEVPHAVAESLNIVIPILAILLTLYPFAIWVESATGRNIPQLIMDFMA
                     PLIAVSDSLGAICLFVIATHLLWFLGINGSLVLMQLWTPFLLQNMAANLAAFQAGEPL
                     PFIITNSFWDFYIVHGASGGVIALAFLLVRSKSAHLRSIGKIGLVPSFFSIGEPIVYG
                     VPMVVNPLFFIPLIFAPLANAVIAYLILDFNLIHRVYLMAPWTTPAPIGAYLVSAGDI
                     WAPILSIALIILDIVIYYPFFKMYEKVCVEKEKAQNLDEKAQQELIEKARMAAQ"
     gene            9760..10584
                     /locus_tag="PWY43_000774"
     CDS             9760..10584
                     /locus_tag="PWY43_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2135673.1"
                     /translation="MNGPTQLAHFQEQIRKEYDGLSKRLKQVAQYVLDNGNSVVFDTV
                     STIAERADVPPSTLIRFANAFGFSGFNEIKQLYRQDMMEGTSRYTERVQLFRQIEPLD
                     EQNSELNHILDVFVQGNTQALQQLSTQISEEQLAQTVDILHNAKRIFIVGLKRSFSIA
                     NYLNYALHHLDYDVFMIDGSGGMFDEQLSRIREGDAVIAVSFSPYANETLNVVTATAR
                     PGVKHIAITDSQLSPLLSFSDVSFIIKEAQVSGFRSQCATMTLAQTIAVSLALKKA"
     gene            complement(10628..10870)
                     /locus_tag="PWY43_000775"
     CDS             complement(10628..10870)
                     /locus_tag="PWY43_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2135674.1"
                     /translation="MSDKSIILEIINALGNKENIKQVEACLTRLRVVLHNNGLLNKSA
                     LKKLGAVDVIKVGDTQQIIFGAKAGQYRDEINALIG"
     gene            11135..11383
                     /gene="rpsP"
                     /locus_tag="PWY43_000776"
     CDS             11135..11383
                     /gene="rpsP"
                     /locus_tag="PWY43_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL2135675.1"
                     /translation="MVTIRLTRGGAKKRPFYQIVVADSRSPRDGRFIERIGFFNPLAA
                     GQAERLRLDVAKVDAWVAKGADLSDRVASLVKEARKAA"
     gene            11419..11946
                     /gene="rimM"
                     /locus_tag="PWY43_000777"
     CDS             11419..11946
                     /gene="rimM"
                     /locus_tag="PWY43_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL2135676.1"
                     /translation="MSEQKIEVVGKLGSTYGIRGWLRLYSSTEQSESIFDYQPWFLKI
                     KGNWQPVELESWRYHSNDLIVKLKGTEDREAAQLLTNAEIGVDLSVFPELEEGDYYWH
                     DLIGCTVVNLEGYTMGTVTEMMETGSNDVLVVRANSKDAFGKQERLIPFLYEQVVKRV
                     DLATKAITVEWDAGF"
     gene            11991..12746
                     /gene="trmD"
                     /locus_tag="PWY43_000778"
     CDS             11991..12746
                     /gene="trmD"
                     /locus_tag="PWY43_000778"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL2135677.1"
                     /translation="MWIGIISLFPEMFKAITDFGVTGRAVKQNLLQIECWNPRDFTLD
                     KHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKKAASAVDGGEAKVIYLSPQGRKLDQTG
                     VQTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
                     PGVLGKQASALEDSFAEGLLDCPHYTRPEVLDGMPVPEVLMSGHHEQIRKWRLEQSLE
                     RTWLRRPELLDSLALTDEQRVLLAKIKKQHKIS"
     gene            12772..13122
                     /gene="rplS"
                     /locus_tag="PWY43_000779"
     CDS             12772..13122
                     /gene="rplS"
                     /locus_tag="PWY43_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL2135678.1"
                     /translation="MSNIIKQLEQEQLKQNLPSFRPGDTLEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTLRKVSNGVGVERVFQTHSPVIDSISVKRKGAVRKAKLYYLRE
                     RSGKSARIKERLGD"
     gene            13278..13634
                     /locus_tag="PWY43_000780"
     CDS             13278..13634
                     /locus_tag="PWY43_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135679.1"
                     /translation="MKQLSIRLPDDEFTFIKTFASAHKLTLQNFVMSAIQAKVAEMSA
                     KNKISEESNIHQPLSKDEKIMLNEAKVFYSARSHLKAENVILPSDFMEQIKSGCGAEK
                     MAEMMGYTKEDWESMK"
     gene            13636..13959
                     /locus_tag="PWY43_000781"
     CDS             13636..13959
                     /locus_tag="PWY43_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135680.1"
                     /translation="MGQITFSNIFTKVIFENVLSASTKEKIKQMLFQSVVALPPLHSE
                     RKMILQLKKQKITIFYQVIEEQSVQILAYQFGGTDKLTGVSKAHFKTLDAIFQMTDEE
                     KEGKF"
     gene            complement(13969..14301)
                     /locus_tag="PWY43_000782"
     CDS             complement(13969..14301)
                     /locus_tag="PWY43_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2135681.1"
                     /translation="MQIEQYQQWVRQFYQQQGWYERSPFMRVTYLAEEIGEVACAVRA
                     IEIGRERPDETEASQQQKRDNLIEELGDVMDNLFVLADKYGISMTEVLERHQAKFEKR
                     YLQKNMEK"
     gene            complement(14394..15590)
                     /locus_tag="PWY43_000783"
     CDS             complement(14394..15590)
                     /locus_tag="PWY43_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL2135682.1"
                     /translation="MNIPFCLPENISPETFLREYWQKRPLLIRNGLPQIVGLFEPEDI
                     LELALEEEVTARLIQCENEKWSVKSSPLVEEDLEELPVQWSVMVQNLEQWSPELGELW
                     QAFGFIPQWQRDDIMVSCSPLNGTVGKHYDEYDVFLVQGYGQRRWQLGKWCDPSTEFK
                     PNQPIRIFDDMGELVMDEVMNPGDVLYVPSRMAHYGVSESEQGLTFSFGLRYPNATDL
                     LENLCKTIEQQADNINSSEFHLPFRLTPNEQPNALLDPKMVKMFKHQLIDLLQNSEQF
                     DELLTHSVATAASSRRYELLQTDNEYYPDEVQEILEAGGWLQQDANVKILYTENPQRV
                     YVNGEWIDELNEAEMALLIRIANGDSISWNKLASKVQDQAELELMLDTICDWLDSGWI
                     ILSEAE"
     gene            complement(15739..17082)
                     /gene="accC"
                     /locus_tag="PWY43_000784"
     CDS             complement(15739..17082)
                     /gene="accC"
                     /locus_tag="PWY43_000784"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL2135683.1"
                     /translation="MLEKVVIANRGEIALRILRACKELGIKTVAVHSTADRELKHVKL
                     ADETICIGPAPSVKSYLNIPMIIAAAEVTNADAIHPGYGFLSENANFAEQVEKSGFIF
                     IGPTADVIRLMGDKVSAINAMKKAGVPCVPGSDGPVGNDPKKNKEIAKRIGYPVIIKA
                     SGGGGGRGMRVVHHEKDLEESIAMTKAEAKAAFNNDMVYMEKYLENPRHIEIQVLADT
                     HGNAVYLAERDCSMQRRHQKVVEEAPAPGITPELRQFIGERCANACREIGYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHPVTEMITGVDLVKEQLRVAAGLPLSITQDDIKVKGHA
                     IECRINAEDPNTFLPSPGKITRLHVPGGLGVRWDSHIYSGYSVPPHYDSMIGKLITFA
                     ENRDIAIRRMENALSETIVEGIKTNIPLHTQILADKEFRKGGTNIHYLEKKLGLK"
     gene            complement(17154..17621)
                     /locus_tag="PWY43_000785"
     CDS             complement(17154..17621)
                     /locus_tag="PWY43_000785"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL2135684.1"
                     /translation="MDIRKIKKLIELVEESGITELEVSEEEGTVRISRAAPAVAPAAV
                     QYAAAPVVAPAAPQVASTAPVAAPAEAPKAAETSGHAVLSPMVGTFYRSPSPDAKPFV
                     EVGQTVKIGDALCIVEAMKMMNRIESDKAGVVKEILVNDGEPVEFDQKLFIIE"
     gene            complement(17874..18656)
                     /locus_tag="PWY43_000786"
     CDS             complement(17874..18656)
                     /locus_tag="PWY43_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2135685.1"
                     /translation="MAVKNEQFIVICNGVFRLFGNIVKILSYPFHALFPKKRFTIPEY
                     SPAKIQSSIPSKITKTIWQTNYSNKVTLPMYANYLFTRLMSLSYDYRYVSTEEREAYI
                     KANADERTFNAYSKLTDGAAQADFWRIFTLLREGGIYIDIDGHLVYPLSKIIGENDSE
                     VLIKRRDKYTNFFLASEAGNWILKDTLELIISNIENRRIEGGVFVMTGPDTLNNAIGD
                     KEVNFRRDKVTCAQGTFTNEYFQYIDKPGSKWNYKKNEDLLK"
     gene            complement(18666..19103)
                     /gene="aroQ"
                     /locus_tag="PWY43_000787"
     CDS             complement(18666..19103)
                     /gene="aroQ"
                     /locus_tag="PWY43_000787"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HDL2135686.1"
                     /translation="MKKILLLNGPNLNMLGKREPQIYGSQTLADIENHLKKLAAAQDV
                     ELESFQANGEEPIINRIHQAFQHTDFIIINPGAFTHTSVAIRDALLSVSIPFVEVHLS
                     NVHSREPFRHHSYLSDIAKGVICGLGAKGYEYALDFAISHLKA"
     gene            complement(19110..19568)
                     /locus_tag="PWY43_000788"
     CDS             complement(19110..19568)
                     /locus_tag="PWY43_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2135687.1"
                     /translation="MAFTIRKMVEQDTHQVFPLMQKLAVFEHYIDSFAITPEVVMESG
                     FRKSPPDFYCLVAENASNIIGILVYYFLPYTAQNRPAIYMKELYVDETARNQKVGEAL
                     MQALKNEAKAHHCKTIKWTVAPWNDGGIRFYQRLGAKENRKWLNYELQVE"
     gene            complement(19628..20452)
                     /gene="dapD"
                     /locus_tag="PWY43_000789"
     CDS             complement(19628..20452)
                     /gene="dapD"
                     /locus_tag="PWY43_000789"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDL2135688.1"
                     /translation="MSLQAIIEAAFERRAEITPKTVDAETKAAVEEVIAGLDNGSLRV
                     AEKKDGEWVVNQWVKKAVLLSFRIADNEIIDGAETKFYDKVPTKYGNYTEEQFKADGI
                     RAVPGAVVRKGSHIEKNVVLMPSFVNIGAYVGEGTMVDTWVTVGSCAQIGKNVHLSGG
                     VGIGGVLEPLQANPTIIGDNCFIGARSEIVEGVIVEDNCVISMGVFIGQSTKIYDRET
                     GEVHYGRVPAGSVVVSGSLPSKDGSHNLYCAVIVKKVDEKTLSKVGLNDLLRSIDE"
     gene            20600..21262
                     /locus_tag="PWY43_000790"
     CDS             20600..21262
                     /locus_tag="PWY43_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL2135689.1"
                     /translation="MENFANLLTGKTREHLVPLPNALSDKHYLQPQVVEAFLTLQHAA
                     KQAGFNLQPASTFRDFERQKGIWNAKFRGERKVHDDNGVAIDMAALSDLEKCKAMLRW
                     SAVPGASRHHWGTEIDIFDPDLLPENTPLLLEPWEYLEGGYFADLTKWLHVNAETFGF
                     YFPFDGKHSRVGFEPWHISYRPISNEYEQRFNDEILKAAWQDEEVAGKECLLEHFEEL
                     LK"
     gene            21259..22011
                     /locus_tag="PWY43_000791"
     CDS             21259..22011
                     /locus_tag="PWY43_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2135690.1"
                     /translation="MIQLINETQQHDKFRQICEKWQLVEDKSSPLALVLTEERLELRK
                     LDEPKLGAIAVNFVDGTMAHRRKFGGGRGEAVAKAVGIKGDYLPTVIDATAGLGRDAF
                     VLAAVGCKVLLVERNPIVAALLEDGLNRAYQDVEIGSFMQERMILADVRDISQLDPQS
                     QMAEVVYLDPMYPHKQKSALVKKEMRVFQHLVGADLDSDSFFLPAKGLAKKRVVVKRP
                     DYAPFLAEQKPDFSHTTKNHRFDVYLSHLKND"
     gene            22142..22978
                     /gene="ftsN"
                     /locus_tag="PWY43_000792"
     CDS             22142..22978
                     /gene="ftsN"
                     /locus_tag="PWY43_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDL2135691.1"
                     /translation="MPQRDYVARSNEKKSKKNQKESNVKKILMRIIGVILFFGIGLWF
                     LKSSAPVPTVPSAPQPANSQTKSELPSRPEETWSYIRDLETRTVITDNSQTSQERLAQ
                     LTAEQKQQIEERRKQEEARLAAEQAQQKPATDVSATELAPKTEEVKSLTEEELAAKKA
                     QEQALAQKQAEEKRRQAELKKQQEAKKEQPAKIVASAATLADKAANQAGKFGLQCGAF
                     KNKAQAENMQARLAMAGFNARIASSADWNRVFVGPIGDRAATSRAQSNARSVAECVIV
                     AM"
     gene            complement(23020..>23742)
                     /locus_tag="PWY43_000793"
     CDS             complement(23020..>23742)
                     /locus_tag="PWY43_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL2135692.1"
                     /translation="FAAPLLIAADGANSQVRQWAGIGLTAWQYRQHCLLATVTTEKPA
                     PSGTWQQFYPTGPRAFLPLSGNHGCVVWYDSPQKIAELKMLPAEKLGVAIEQHFPSRL
                     NAELGNIQVQASGAFPLTRQHAQHYVQNGVVLVGDAAHTINPLAGQGVNLGFKDVKTL
                     LEVIEQAVKKGKNFASEQVLQRYEQKRKPDNLLMQTGMDVFYKAFKTDLLPLKIARNV
                     GLVLADKVSPIKKQALKYAIGI"
BASE COUNT         6706 a         4996 c         5174 g         6866 t
ORIGIN      
        1 tgagctgcct aaaattgaag atatacacaa atacgaaatg ctttcaatat taaaaataca
       61 agcagctaag gaaaataatg cagagttgag atactatgtg aaaaagaaga aaaaatgaga
      121 gtcagaatat caatatctcg acgtttttta ggtattatct tctttttgat tattcatatc
      181 tgtttggcaa aagaatgggg gaattaccct cttgggatta gattcacttt tgaatatatt
      241 ggaatgcaaa cagaaaattt taaatatttt attggtattg tattaactct tttgataatt
      301 ccagccttct atttatttaa acaatcaata aaaattgagc acgattatat ttatttccca
      361 aaagataata aataaaaact caaacggtca gttttacaaa tttttctgca aaatgcttgc
      421 gtttgaacat cggctatgcc gatggcacga agtgcgagtg tagcgagtaa aaccgaccgc
      481 ttgtatttat gatcaattac gccacttcac tcaatttcac tggtcggttt tcttccagtg
      541 attttttcgc agctaaggca ataagtaccg gttgtaagcc gtcattgccg tttactaacg
      601 ttggtttgtc gtttaccaca gaatctacga aacagctgat ttcatcggcg aaagattgca
      661 tataacgttc taagaagaag tattttggtt tttccgccac cacgccgttt tcacctgaga
      721 atacggcagt tgagccggta tcattagtga tatggattgc ccctttcgag ccgaacactt
      781 ccgcacgttg gtcgtagccg taggcagctt tgcgactgtt gtcgattacg ccgattgctc
      841 cgtttttcag tttcaacgtg ataacggcag tatcaatatc gcccgctttg ccgatttcag
      901 ggtttactaa tacggtgccg actgcaaaca cttcttccac ttcgctgccg gataaataac
      961 gaatcatatc gaagtcgtga atggtcatat caaagaacat tccgccggag actttcacat
     1021 attcgattgg cggggcttcc ggatcacgag aggttactcg gataacgtgc ggttcgccga
     1081 tgtcgcctgc cgccacacgg tctttaatcg ccttgaagtt gtggtcgaag cggcggttaa
     1141 agcccacttg gaattttaca ccggcttttt cgactgcact caatacttca cggattttag
     1201 ctgggtctgc atctaccggt ttttcacaga aaacgtgttt gccagcctgt gctgcctcaa
     1261 tcgaaatcgg agcgtgagtg ttggtggaag agcagaccaa taccgcatca atttccggat
     1321 cttgcaggat ttttttatag tcatcataaa cttccggaat acccatcgat tttgcccaag
     1381 cggtcaattc ttcggtaatt tttacatcag agattgcttt gatttcagca cctttgacat
     1441 atttgctaat actttcagaa tgcacacgtc caatacggcc tgcaccaata atacctactt
     1501 ttaacatatt aaactcctgc ggtttcacgg atatattttc tgcctttgag ggcgtattca
     1561 aacgggttgg caagtgccgg atcttgttcg gcttctacaa ccatccagcc tttgtagtcg
     1621 tgtttttcta aaatatcgaa aatcggacgg aaatcaatga caccatcgcc cggtacagta
     1681 aaggtgcctt ttttcacgcc ttctaaaaag cttaaatctt ttgcttttac ttccgccacg
     1741 acttcatcac gcacatcttt taagtgaacg tggataatac ggtcgatata tttttccagt
     1801 acgccgagca tcgctttttg gcagccttcg gaatagtaca aatgccctga atcgaacaac
     1861 agatagacat cgtcattcac ttctgccata tagcgatcga cctcttcggg tgtttgaata
     1921 ccggttccca tatggtgatg caggcagact ttcattcctt tttcggcggc aagtttcgcc
     1981 aattcgttat agccttctgc catacgtttc caatcttcat cgttgaaaat cggtttttct
     2041 ttgaacacag atttggttgt gccttgaata ctacggctct gttcggaaca gccgatcact
     2101 tttgccccca ttgcatgcaa gaaatcacga tgtttgatga actcggcaat ggtctcttcc
     2161 cgtttaccgt ccacaaagaa ggtgctgaac cacgcattac agatttgaat gccacgcacg
     2221 tccagtttgt gttttaacac ggcaacatca cgcggatatt tattacccac ttcacagccc
     2281 gtgtagccgg caagtgccat ctcactcaca cactgctcga aagtgttttc cgctcccagt
     2341 tccggcaggt catcgttcgt ccaaccgatt ggggcaatgc ctaattgaat gttttcagct
     2401 ttcatagaga aacctcgttt gatttataga gataaatttg gatgcaagcg gttattttta
     2461 tcaaaaattt tgcaaatttg accgcttgat attctcccct cgtagctcaa agtggggagt
     2521 tgttttttta actaatactt ccgagcctta gctaactgct cttcggagcg ttggcgggct
     2581 tgttgaatac gctcgctctc tgacacttcc gcgacaccaa cgtgccacca gcagccatag
     2641 ccgtgagcca ttgttttcgg caacacttta atatcgatta aggtagaaac ggtctgtttt
     2701 ttcgcatcat caagggcggc acgcaattcg gcttcgttag ttacccgata ggttttgcag
     2761 ccgtatgctt cggcgttttt ggcaaaatca atcggcacta aaccgccgtt gagttggtta
     2821 gtttcgccat tgcggaagcg gaattccgta ccgaaactgt ccataccgtt gccgatttgc
     2881 aggttgttga tacagccgtt tgccatattg tcgaacagca ccacgttgat ttttttgccc
     2941 tcttgaattg aggtcactaa ttcggtgtgt gccattaagt aagagccgtc ccccagcaag
     3001 gcatagacct cttgttcggg gcgggcaatt ttcacgccga ctgccgcact cacttcatag
     3061 cccatacagg agtagccata ttcaaggtgg taagtctctt cacctttact taaccaagtg
     3121 cgttgtaaat cacccggcaa acttccggcg gcggcaacaa taatcgcatt gttatcaatg
     3181 gtttcattca caatacccag cacttgggtt tgggtgagct gggaaccggt gagctgaata
     3241 aattcattgt gaaccgcttt atgatcaaga ttatcgttaa tttcaggggt gaaatcaggg
     3301 tgatattgca cagcgcgcag gcgttgaatt tcatcagcaa aatccgcttt cgcctgtgaa
     3361 atttcattct cccattgagc ttgataatca gtaggaatgg cttggcttaa cgcctctaat
     3421 gcagtttttg catcggcaac cagttgcacg ccgtctaatt tgtacgcatc aaaacgtgcc
     3481 acattaatgt tcaaaaactg cacgtttggg ttttggaaaa tccatttgga agaagtcgtg
     3541 aagtcggtgt agcgtgtacc aatgccaatc actaaatccg cttcttttgc cagacggttt
     3601 gccgctaaac aaccggtttc tcccactccg cctacattca atgcgtgatc ggacaccacc
     3661 gcacttttac ccgcttgggt ttcagcaaat ggaatgccga actgtttagc aaatttttgc
     3721 agaatttgac ccgcttgcga gtagcgaaca ccgccaccgc agacaatcag cggttttttc
     3781 ttggtttgaa ttaatgttac cgcttgctta atcatcgctg ttgtcggcgg tgtacgctct
     3841 aagcgatgca ctcgtttttg caggaagctg ttcgggaaat caaaactctc cgcttgtaca
     3901 tcttgcggca gagcaatggt gacagcaccg gtttcagcag ggtcggtcag cacacgaaac
     3961 gcattgatac aagcactcat taactgctca gggcgttgca ctctatccca atatttactg
     4021 acggcacgga acgcatcgtt agtgctaatg cttaaatcgt aaggttgttc gatttgttgc
     4081 agtaccggat ccggctggcg ggtcgcaaac acatcgcccg gtaataaaag aagaggaata
     4141 cggttcgctg ttgccgttgc cgcagccgta accatatttg ccgcccccgg cccgaccgag
     4201 gaagtacagg caataattct ttgacgcaga ttttgcttag caaagcccat cgccaaatgt
     4261 gccatacctt gctcatttct gccttggtag agcgttaaac caccggcatt ttgagcaagc
     4321 gcctgaccta ttcccagcac attgccgtgg ccgaaaatag cggcaacacc gtgaacgaat
     4381 ttaatctctt tgccgtccac gtctaaatat tgattatcca aaaattgcac taacgcctgt
     4441 gcaaccgtta aacgagtagt ctgcataaag cctcctctgg cttacatcgc ttttgcaagc
     4501 ggtcggtttt ctactgattt ttacaaatga aaaatttatt tcatttataa tagcaaggaa
     4561 atcgaaaaaa agatgtgatc cagttctcat ttctgcgaag ttattgcctt atttttcaaa
     4621 gtgagagcta gatcacagaa atgataattt aaatagaata aatttgaaat aaaaatttca
     4681 tttttgtatg atgtatagtg tgtattcaaa tttacttgtt gtaaggagcg gttatggcaa
     4741 cagttaatca tcaagatcgt ccattggatc ttatctgttt agggcgtgtg gcggtggact
     4801 tttatgggca acaaatcggt tcccgtttgg aagagatgac cacattttca aaatacctcg
     4861 gcggctcgtc aggcaatgtg gcttacggta cggcggtgca aggtttacgt tcttcaatgc
     4921 tggcaagggt cggtgacgaa catatggggc gttttctacg tgaagagcta caaagcgtag
     4981 gcgtggacac cagccattta attaccgata aagaacgctt aactgcactg gttattctcg
     5041 gcattaaaga taaagagact tttccgctga ttttctatcg tgataactgt gcggatatgg
     5101 caatcaccaa agacgatttt agcgaagcct atattgcttc cagccgttgc ttagcaatta
     5161 ccggcacgca cctgtcaaac cctaaaaccc gtgaagctgt gctgaccgca ctcgaatatg
     5221 cgggtagaaa tgaggtaaaa cgagcgttag atattgatta tcgccctgtg ttatggggat
     5281 taacctcact cggtgatggc gaaacccgtt atatagacag tgccgaagtc accaaatcgc
     5341 tacaagaagt attgcaccat ttcgatctga ttgtcggcac agaggaagaa tttcatatcg
     5401 caggcggttc gaccgatact ctcactgcac tcaaaaatgt gcgtaaagtc tctaatgccg
     5461 tattagtgtg caaacgtggg gcattagggt gttcagtatt tgaaggcgag ctgccgaatg
     5521 atttagacgg tggcttgaat gtgtacggcg tgcgtgtaga agtgctgaat gtgctgggtg
     5581 caggcgatgc ttttatgtcg ggcttactgc gtggctactt aaataacgaa ggttgggaac
     5641 aagcgtgcca ctatgccaat gcgtgcggag cgttagtggt atctcgtcac ggctgttcac
     5701 cggcaatgcc gaccaaacgt gaacttgacg attatttaac ccgtgcagag agtgtaccac
     5761 gtccggattt agatgagcgt ttaaaccact tacaccgtgt caccacccgc aaaaaacagt
     5821 gggaaaatct ctgtattttt gcttttgatc accgcaaaca gttggtggat atggcaaata
     5881 aagtcggtgc agatcttgct cgcataccga aactgaaaca gctgctatta aaagcgatgg
     5941 aacaaaccgc aactcaatcc ggcttaccac aaaatcaagc agggatccta gccgatacca
     6001 ctttcgggca agatgtactt aatgagatta ccggcaaaga ttactggatt gcccgcccga
     6061 ttgaagagcc gtcttcccgc ccgctggaat tagagtatgg agatttaggc tctcagctag
     6121 catcattccc acgcgatcac gtcatcaaat gcttggcatt ttaccacccg aatgatgaaa
     6181 gcggtttaaa agcccgccaa gatcgcaagt taaaagaggt gtatcaagcc tgttgccgta
     6241 ccggtcacga gctattgttg gaaattattc tgcctgctga tatgccacag agtgaggctt
     6301 tctataacca agcgatagca catttctacc aacttggcat taagccggaa tggtggaaat
     6361 tgccggatgt ttctcgcgca caatggcaag cgatttccgc caccattgag gcaaacgatc
     6421 cggactgtcg tggtatttta attttaggtt tagatgcccc gttagaggtg tttaaagaca
     6481 cctttgaaca ggcaaaaggc tgtgagaaag tgaaaggctt tgcagtcggc cgcaccattt
     6541 tcggtgaacc gtccgaaaaa tggcttgccg gtgaattgga cgacaatacc ttgatttctg
     6601 cggtatcgga gaaatatcgc actttaatcg atctctggca acaacgttaa tcatattgct
     6661 ttaagtgaaa accccttagc ttatgatatt aagcaagggg ttttgttttg caaaaaatcc
     6721 ggctaaacag accgcttgct agccgtaaaa atgcccgaat tttcctaaaa aagcgaatat
     6781 cgggcattcc tcaatctgct tatcacaaaa taagtgtgat gaagttcaca tttcatacat
     6841 aaaccccccc ctttttatcg aaacaaattg ttttattttt atcaaaatgg aaaatgtatt
     6901 tcagtttagt tctatttaga ttcaatagga gtctgtgtat gagttgcaac acttcaccaa
     6961 aacgtttcca cgccagttgg gagtcgatca aacagcacca agtaccagag tggtatgaag
     7021 atgctaagtt tgggattttt attcactggg ggatttactc cgtacccgcc tttgccccac
     7081 cgacctgtca gttaggtgaa attgatattg atgaacagtg gttctgtaat aacccttatg
     7141 cagaatggta ttttaattcc atcaatgtca aaaaaggccc gacctacgaa catcatatca
     7201 aaacttatgg cgaatccttt gcctataacg acttcctgcc actatggaaa gcggaaaaat
     7261 ggcaaccagc agaatgggcg aaactgtttg ccaaagctgg cgccaaatat gtggtattaa
     7321 ccaccaaaca tcacgatggt ttctgtctct atccaagtaa atataccgat ttcaactgca
     7381 tgcaagccgg accgaagcgg gatttaatgg gcgagctgac tgaaaatgtg cgggagcaag
     7441 gcttaaaaat gggcgcttac tactcgggca ttatcgattg gacttattcc tctgccccga
     7501 ttttcaccga aagtcaaaac ttctcaaacg cttgcccgac ttacgaatac gctgactacg
     7561 cctataaaca agtggtagag ctgatcgacc gctataaacc agatgtattg tggaatgata
     7621 ttggctggcc aaaagtgggc gagcatatgt taccacatct atttgctcat tattacaaca
     7681 gcgttcaaga gggcgtagtg gacgaccgct ggaacaaact atggtgcgat ttcacctcca
     7741 aagaatacaa acacggcgtg gcttcccgtg ataagaaatg ggaaatgtgc cgtggtatgg
     7801 gcttatcgtt cggttataac caggtagaag acgaaagcca tttgatttcg gtgaaagact
     7861 tgatttccct attggtagaa accgtagccg acaacggcaa tttattgctg aatatcggcc
     7921 cgaaagcaga tggcactatt ccgcaagagc aagttgaacg cttattagca ctggggagct
     7981 ggctggaaat aaatggagag ggaatctata aatcccgttg tagccaacac caaaccgagc
     8041 aaatcggtga tgtaacctat cgttatacca aagttggggc ggatctctat ctttacattg
     8101 atggcttaac cgaagcccaa atcagcttgc cactgtcagt ttatggtgag ctacaagcac
     8161 tcaataccga cctgaaattt accattgaaa acactgcaca aggcagagtg ttacatattc
     8221 aaaactacca accggattgg tatgtattag gctttaaaat tgcagatggg gagaaatagg
     8281 tatgtctgtt tcacaacgct ttattaactt tatgaatacc cacgttgccc cagtggcacg
     8341 ccgaatggaa aatcaaccgc atatttccgc tattcgagac ggttttattg tagtgttacc
     8401 attcttaatt gtcggcagct ttattatgat tttgctaatt ccgccatttg atgaaaatac
     8461 ccaaaatgct ttcggtcaag cttggtggcg ttttgctaac tgggcaagtc cttacggttg
     8521 gaatttcttc caaatgtcgt ttaatgcgat ttcattattt acctctgcca gtatcgccta
     8581 caaccttgcc aaagcctata aaagagagcc cctgccagcg gcatttctct cggtaatggc
     8641 atttttgtta gtggctgcac cggtcaaaga cggaatgatg gatattaaat tctttggcgg
     8701 tattgggcta ttctctgcga tttttattgc catttattcg gtagaaatga cccgcttgtt
     8761 ggagttctta aaaattaaaa tccgcttacc gaaagaagtg ccgcacgctg tagcagaatc
     8821 tctgaatatc gtgattccaa ttttagcgat tttactgact ctctatccgt ttgccatttg
     8881 ggtggaaagt gccacaggtc gtaatattcc acaactaatt atggacttta tggcgccatt
     8941 gattgctgtg agtgactcgc taggggcaat ttgcttgttc gttattgcta cccatttgct
     9001 ctggttctta ggcattaacg gctcattggt actaatgcaa ctttggacac cattcctact
     9061 gcaaaatatg gcggcaaact tagcagcatt ccaagctggt gaaccgctcc cctttatcat
     9121 caccaattcc ttctgggatt tctatatcgt acacggggca agtggtggtg taatagcctt
     9181 ggcattcctg ctggtacgca gtaaatcggc acacttacgc tctatcggta aaatcggctt
     9241 ggttccgtcc ttcttctcta tcggtgaacc aattgtgtat ggtgtgccaa tggtggttaa
     9301 tccgctgttc tttatcccgc tgattttcgc accactagcg aatgcggtta ttgcctacct
     9361 gattttagat tttaacctta ttcaccgtgt ttacttgatg gcaccgtgga caacaccggc
     9421 accgattggg gcatatctgg tttctgccgg tgatatttgg gcaccaattt taagtattgc
     9481 actgattatt ttggatattg tgatctacta tccattcttc aaaatgtatg aaaaagtctg
     9541 cgtcgagaaa gaaaaagcac aaaatctaga tgaaaaagca caacaggagt tgatagaaaa
     9601 agcacgaatg gcagcacaat aacacttatt tctagcgtaa ttgagagggg ttaatcgaaa
     9661 gattagccct tatttttgca aaaaattgac aaaatttatc cgcttgtaaa caagaaaagc
     9721 gtataatgaa ataaaaaatt caagcagaga taatttgaaa tgaatggacc tactcaatta
     9781 gcacattttc aagaacaaat ccgaaaagag tatgacggac taagtaaacg tttaaaacaa
     9841 gtggcacagt atgtgctgga taatggcaac agcgtggtgt ttgatacggt ttctaccatt
     9901 gctgaacggg ctgatgttcc tccttcaacc ttaatccgtt ttgcgaatgc atttggattt
     9961 agcggtttta atgaaattaa acagctttat cgccaagata tgatggaagg cacatcacgt
    10021 tacaccgaac gtgtgcaact ttttcgtcaa attgaaccgc ttgatgagca aaacagtgag
    10081 ctcaatcata ttttagatgt gttcgtacag ggtaatacgc aggcactgca acagttatcg
    10141 acacaaatca gcgaagagca gttagcacaa acggtggata ttttacacaa tgctaaacgc
    10201 atttttattg tgggcttaaa acgctcattc agcattgcga actacctaaa ctatgcgtta
    10261 caccatttgg attatgatgt ttttatgatt gatggttccg gcggtatgtt tgacgagcaa
    10321 ttaagccgaa ttcgtgaagg tgatgcggtg attgccgtga gcttttctcc gtatgcgaat
    10381 gaaacgctca atgtggtgac agcaaccgcc agaccggggg tgaaacacat tgcgatcacc
    10441 gatagccaac ttagcccgct cctttcattt agcgatgtgt cgtttattat taaggaagca
    10501 caggtgagtg gttttcgatc ccagtgtgct acaatgacgc ttgcacaaac gattgccgtt
    10561 tcactggcgt tgaaaaaagc ctagattaag aaagtgaaag acctgcgtaa atgcaggtct
    10621 ttggttatta gccgattaaa gcgttaattt catcacgata ctgcccggct tttgcaccaa
    10681 aaataatttg ctgcgtatca ccgactttaa tgacatcaac cgccccaagc tttttcagtg
    10741 ccgacttatt taataaaccg ttgttatgta acaccacacg taaacgggtt aaacaagctt
    10801 caacttgctt gatattttct ttgtttccta aagcattaat aatttctaga atgatagatt
    10861 tatcagacat atttcctcca tacctattta tatttaaaac tcttattatg atattttaaa
    10921 cattttctga tggttagaac aatttttatt agtaatttgt ataaaaaaac gctaaatgta
    10981 tctttcttta aaagagtcat atcaatgaat ctcccttgtc tttccgcaaa aaatctgtat
    11041 aatatgaccg cttgttttct cttttgaaac aagttcgtca ccctcacctc gaacgagggt
    11101 ttatttttta ctatcaacat ttagaggaaa ggttatggta accattcgtt taactcgtgg
    11161 cggagctaaa aaacgcccat tttaccaaat cgtggtagcg gatagtcgtt cacctcgtga
    11221 tggtcgcttt atcgagcgta tcggtttctt caatccatta gcagctggtc aagctgagcg
    11281 tttacgttta gatgtggcta aagtggatgc gtgggttgct aaaggtgctg acttatcaga
    11341 tcgtgttgct tctttagtaa aagaagctcg caaagcagct taatattaaa taagccaaaa
    11401 ttaaataagt aggtgagtat gagcgaacaa aaaattgaag ttgtcggtaa attaggttca
    11461 acctatggga tccgtggctg gttacgcctc tattcatcaa cagaacaatc tgaaagcatt
    11521 ttcgactatc agccgtggtt tttaaaaatt aaaggcaact ggcagccagt cgaattagaa
    11581 agttggcgtt atcacagcaa cgatttgatt gtgaagttaa aaggtacgga agatcgtgaa
    11641 gccgcacagt tattaacgaa tgccgaaatc ggtgtagatt tatctgtatt tcctgaattg
    11701 gaagaagggg attactattg gcacgattta atcggctgta cagtagtaaa cttggaaggc
    11761 tacacaatgg gaactgtaac cgaaatgatg gaaacaggtt caaacgatgt attagtggtg
    11821 cgtgcaaaca gcaaagatgc ttttggtaaa caagaacgat taattccgtt tttgtacgaa
    11881 caagtagtta aaagagtaga tctcgccacc aaagccataa cggtggagtg ggacgctggt
    11941 ttctaacctc aggtatgcgg tgagctttgg aaaacatttg aggcgttaag atgtggattg
    12001 gtattatttc actgtttcct gaaatgttta aagcaattac cgatttcggg gtgacaggac
    12061 gtgcagttaa gcaaaatcta ttgcaaatcg aatgttggaa tccacgtgat ttcacattag
    12121 ataaacataa aacggttgat gatcgtcctt atggcggtgg gccgggaatg ttaatgatgg
    12181 tgcagccgtt gcgtgatgcg attcacgcag caaaaaaggc agcatcagca gtagatggtg
    12241 gtgaagctaa agtgatttat ctttcaccgc aaggacgtaa actcgatcaa acaggcgtgc
    12301 aaacgctggc ttcaaatcag aaactgattt taatttgtgg acgatatgaa ggcgtggacg
    12361 agcgattgat tcaaacagaa gttgatgaag aatggtcaat cggtgattac gttctcacag
    12421 ggggggaatt acctgcaatg acattaattg atgctgttgc aagatttgtt ccgggcgtat
    12481 tgggtaagca ggcttcagcg ttagaagatt ctttcgcaga aggattacta gattgcccac
    12541 actatacccg ccctgaagta ttagacggta tgcctgttcc ggaggtgctg atgtctggac
    12601 atcacgaaca aattcgtaaa tggcgattgg aacaatcgct tgagcgaacg tggttaagac
    12661 gccctgagct attggatagc ctagctctga ctgacgaaca aagagtgttg ttagctaaaa
    12721 ttaaaaagca acataaaatc agttaattct aggatttaga aggttttcaa aatgagtaac
    12781 atcatcaaac aacttgaaca agaacagtta aaacaaaact tacctagctt ccgtccaggt
    12841 gacacattag aagttaaggt atgggtagta gaaggtagta aaaaacgttt gcaagcgttc
    12901 gaaggcgtgg ttattgcaat tcgtaaccgt ggcttgcact ctgcattcac tctacgtaaa
    12961 gtttcaaatg gcgtaggcgt tgagcgtgta ttccaaactc actcacctgt aattgacagc
    13021 atttctgtta aacgtaaagg tgcggtacgt aaagctaaac tttactactt acgtgagcgt
    13081 tctggtaaat ctgcacgtat caaagagcgt cttggcgact aattgctaca aaacagtgaa
    13141 atgcacaaaa gccttgagga aactcaaggc ttttgctttt tgtatttaca agcagttatt
    13201 tttcctcaat gatttacaaa tcttgctttc ttttttgtta taatgttaac aagataacat
    13261 taatgatagg tgtcgatatg aaacaattaa gtattagatt gccagacgat gaatttactt
    13321 ttattaaaac atttgcttct gctcataaac tgactttaca aaattttgtg atgtccgcaa
    13381 tccaagctaa agttgcagaa atgtctgcta aaaataaaat atctgaagaa agtaacattc
    13441 atcaacctct ttctaaagat gaaaaaataa tgctaaatga ggcaaaagta ttttattctg
    13501 ctcgttcgca tttaaaggca gaaaatgtga ttcttccgag tgattttatg gagcaaatta
    13561 aatcaggctg tggagcagaa aaaatggcag aaatgatggg atataccaaa gaagattggg
    13621 agtcaatgaa gtaatatggg gcagattaca tttagtaata ttttcacaaa agtgattttt
    13681 gagaatgtgc tttctgcttc aactaaagag aaaataaaac aaatgctttt tcagtctgtg
    13741 gttgcattac ctcctctcca ttcagaaaga aaaatgattt tacagttgaa aaagcaaaaa
    13801 attaccattt tttatcaagt tattgaggaa caatctgtgc aaatcctcgc ttatcaattt
    13861 ggtggaactg ataaattgac aggtgtgtct aaagctcact ttaaaacact agatgccatt
    13921 tttcaaatga cggatgaaga gaaagaagga aaattctaat gtaagcggtt atttttccat
    13981 atttttttgc aaataacgtt tctcaaattt tgcttgatgg cgttcaagca cttccgtcat
    14041 cgaaatgcca tatttatcgg ctaatacaaa aagattatcc atcacatcgc ctaactcttc
    14101 gattaagtta tctcgttttt gctgttgtga agcttcggtt tcgtccggtc gttcacgccc
    14161 gatttcaatc gctcttactg cacaagccac ttcgccgatt tcttctgcta gataagtcac
    14221 ccgcataaaa gggcttcgtt cataccaacc ttgttgttgg taaaactggc gaacccattg
    14281 ttgatattgt tcaatttgca ttttatttct gattcaaaaa gtagggacga aaaatttttc
    14341 gcccttttat aagaaaatgt atcagggcaa atgatgattt gcccctagga gtgttactct
    14401 gcctcagaga gaataatcca gccactatcc agccagtcgc aaatggtgtc tagcattagc
    14461 tccagctccg cttggtcttg cactttggag gcaagtttgt tccacgaaat cgaatctcca
    14521 ttggcgatac gaatcagtaa tgccatttct gcttcgttta actcatcaat ccactcgcca
    14581 tttacataaa cccgttgagg gttctcggtg tagaggattt ttacatttgc atcttgctgt
    14641 aaccagccgc cggcttcgag aatttcttgc acttcgtcag ggtaatattc attgtcagtt
    14701 tgcaataatt cataacggcg ggagcttgcc gcagtggcaa cactgtgagt gagtaactca
    14761 tcgaactgtt cggaattttg gagcaagtcg attaactgat gtttaaacat tttcaccatt
    14821 ttcggatcaa gcaacgcatt cggctgttca ttcggcgtta agcggaacgg aagatgaaat
    14881 tccgatgaat taatgttgtc ggcttgttgt tcgatggttt tgcagagatt ttccagtaaa
    14941 tcggtcgcat ttggataacg taaaccaaaa gagaaagtta aaccttgctc gctttcggaa
    15001 acaccatagt gagccattcg agacggaaca taaagcacat cacccgggtt cattacctcg
    15061 tccattacca gttcgcccat atcatcaaaa atgcggatcg gttggtttgg tttgaattcg
    15121 gtgcttggat cgcaccattt gcctaattgc caacgacgtt gcccatagcc ttgcaccaag
    15181 aacacatcgt actcatcata atgtttgccg acagtgccgt tgagcggtga gcaagaaacc
    15241 ataatgtcat cacgttgcca ttgcgggata aaaccaaaag cctgccaaag ctcgcctagc
    15301 tcaggagacc attgctcgag gttttgcacc attacagacc attgtacagg taactcttcc
    15361 aaatcttcct caacaagcgg gctggattta acagaccatt tctcgttttc gcattgaatc
    15421 aggcgtgcag tgacttcttc ctctaatgcc agttctaaaa tatcttccgg ctcaaacaat
    15481 ccgacaattt ggggtaagcc gttacgaatt aatagcggtc gtttttgcca atattctcgc
    15541 aaaaaggttt cagggctgat attttcaggt aagcagaatg gaatgttcat aatggctcct
    15601 tgctacgtgc ttctttgaga ttactgatat tctacccgag aaatcaggaa aaacgggaaa
    15661 ttttttatga tttagcgaca tcattcacat tttgaagagg aaataatgaa aaaggcgaac
    15721 attgttcgcc tttgaaattt attttaaacc gagttttttc tcgagatagt ggatattggt
    15781 gccacctttg cggaattctt tatccgcaag aatttgagta tgtaaaggaa tattggtttt
    15841 aatgccttcc acaatggttt cggaaagggc attttccatc cggcgaatcg caatatcacg
    15901 attttcagca aaggtaatca atttaccaat cattgaatcg taatgtggcg gcacgctata
    15961 gcctgagtaa atatgtgaat cccaacgcac acctaaacca cccggaacgt gtaaacgggt
    16021 aattttaccc ggcgatggca agaaggtgtt tggatcttct gcgttgatac ggcactcaat
    16081 ggcgtgacct ttcactttaa tatcatcttg tgtgatcgaa agtggtaagc cggctgccac
    16141 acgcagttgc tcttttacca agtccacacc ggtaatcatt tcggttacag ggtgttctac
    16201 ctgtaagcgg gtgttcattt caatgaagta aaactcgccg ttttcatata agaactcaaa
    16261 agtacctgca ccacggtagc caatttcacg gcaggcattg gcacaacgct ccccaataaa
    16321 ttgacgcaat tcaggtgtaa tacccggagc aggtgcttct tccaccactt tttggtggcg
    16381 acgttgcatt gaacagtcac gctctgctaa ataaaccgca ttaccgtgag tatcggcaag
    16441 tacttggatt tcaatatggc gtggattttc taaatatttc tccatataca ccatatcgtt
    16501 attaaatgcc gctttcgctt cggctttcgt cattgcgata gattcttcca aatctttctc
    16561 gtgatgaacc acacgcatac cacgaccgcc accaccacct gatgctttga tgatcaccgg
    16621 gtagccgata cgtttagcga tttctttatt tttcttcgga tcgttaccaa ccggaccgtc
    16681 agaacccggt acgcaaggca cgccggcttt tttcattgca ttgatagcag agactttatc
    16741 gcccattaag cgaatcacat cagccgttgg accaataaag ataaagcctg atttctcaac
    16801 ttgttcggca aagtttgcgt tttcagataa gaaaccgtag cccggatgaa tagcatcggc
    16861 attcgttact tcggcagcgg caataatcat cgggatattt aaatagcttt ttaccgatgg
    16921 agcaggaccg atacaaatgg tttcatcggc taatttaacg tgttttaatt cacggtcggc
    16981 agttgaatgc actgcaaccg ttttaatgcc caattcttta caagcacgca aaatacgcag
    17041 ggcaatttca ccacggtttg cgataacaac tttttctaac atagggaaat tccttgtgtt
    17101 ctagaaaatg tagggcgtgt tgcacacgcc cgttggataa tttaaggaag agcttattca
    17161 atgatgaata atttttggtc gaattctacc ggttcgccat cattaactaa gatttctttt
    17221 accacaccgg ctttgtctga ctcgatacgg ttcatcattt tcattgcttc tacaatacat
    17281 aacgcatcac cgattttcac ggtttgaccc acttcaacga acggttttgc atctgggctt
    17341 gggctacggt agaaagtccc caccattggt gaaagcactg cgtggccact cgtttctgcg
    17401 gcttttggtg cttcagccgg agctgcaaca ggtgctgtcg aagctacttg aggtgccgct
    17461 ggtgcaacaa caggagctgc tgcatattga actgcagctg gtgcaacagc tggagcagca
    17521 cggctaatac gcaccgtacc ttcttcttca gacacttcta actcggtaat gccagattct
    17581 tcaactaatt caataagttt tttgattttg cgaatatcca taataagatt ccgtatgtga
    17641 ttaaataaaa taaaaaagag aactccacat ttggggtata aattcaaatg tagagttaaa
    17701 aaatgctggg cgtagaatac ctgaatttcg ctaattttgc gaataaaatc tgataatttt
    17761 ggcgaaaatg gacgatttta gcattcatcg taccagttag gatttttgga aaatggtgac
    17821 tcacaagcga ttgaaaattg ccaatttttt gtaaaaaaca accgcttgtc agattatttc
    17881 aataaatctt catttttctt gtaattccac ttgctaccgg gcttgtcgat atactgaaaa
    17941 tattcgttgg taaacgtacc ttgagcacaa gttactttgt ctcggcggaa atttacttct
    18001 ttatcaccaa tggcattatt tagggtgtca ggaccggtca taacaaacac gccaccttca
    18061 atccgacgat tctcaatatt gctgataatc agctcaaggg tgtcttttag aatccaattt
    18121 ccagcttccg aagccaagaa aaagttggtg tatttatcac ggcgtttaat taacacttcg
    18181 ctatcatttt cgccaataat tttagagagt gggtaaacca aatgcccgtc aatatcaata
    18241 taaatgccac cttctcggag caaagtaaaa attcgccaaa aatcagcttg tgccgcacca
    18301 tcggttaatt tgctataggc gttaaaggtg cgttcatcgg cgttggcctt gatgtatgcc
    18361 tctcgttctt ctgtgctaac gtagcgataa tcgtagctca atgacattag gcgggtaaac
    18421 agataattcg catacattgg caaggtgact ttgttagaat aattcgtttg ccaaatggtt
    18481 ttggtaattt tgctcggaat gcttgattgg atttttgccg ggctatattc cggaatggtg
    18541 aaacgttttt tcggaaaaag tgcatggaac ggataactca atattttgac gatattaccg
    18601 aataaacgga agactccgtt acagatcaca ataaattgtt catttttaac agccatttta
    18661 ttaccttatg cttttaagtg tgaaattgca aaatccaacg catattcata gccttttgcc
    18721 cctaaaccgc aaataacgcc ttttgcaata tcacttagat aggagtgatg gcgaaaaggt
    18781 tcacggctat gaacgttaga caaatgcacc tcaacaaacg gaatcgacac actcaataac
    18841 gcatcacgaa ttgccacgct ggtgtgagta aatgccccgg ggttaataat aataaaatcg
    18901 gtatgctgaa aggcttggtg aatacggttg ataatcggct cttcaccgtt ggcttgaaag
    18961 ctctccagct caacatcttg ggccgcagcc aattttttta agtggttttc aatatccgcc
    19021 aaagtttgcg aaccgtaaat ttgtggctca cgtttgccca acatatttaa attcgggccg
    19081 ttaaggagaa ggattttttt catatttact tattcaactt gtaattcata attaagccat
    19141 ttacggtttt ctttcgcccc caagcgttga taaaaacgaa taccaccgtc attccaagga
    19201 gcaactgtcc attttattgt cttacaatga tgtgccttcg cctcattttt caaggcttgc
    19261 attaacgcct ctcccacttt ctgattccga gcagtttcat caacataaag ctctttcata
    19321 taaatagcgg ggcggttttg ggcagtgtaa ggaagaaaat aataaaccaa tatgccgata
    19381 atgttagacg cattttcagc cactaaacag taaaaatcgg gtgggctttt gcgaaagccg
    19441 gattccatta caacttcagg agtaatcgca aaagaatcga tatagtgctc aaacacagcc
    19501 agtttttgca tcagagggaa cacttggtgg gtatcttgtt cgaccatttt gcggattgta
    19561 aatgccataa aattccttac aagcggtaaa aaaatccctc acttttgcaa agcgagggag
    19621 gttttaatta ttcgtcaata gaacgtaata agtcgtttaa gcccactttg cttaaggttt
    19681 tctcatccac ttttttcaca atcaccgcac agtagaggtt gtgtgaacca tcttttgatg
    19741 gaagcgagcc tgaaaccaca acagagcctg ccggcacacg gccgtaatgt acttcgccgg
    19801 tttcacggtc gtaaattttg gtagattgac cgataaacac gcccattgag atcacgcagt
    19861 tatcttcaac aatcacgcct tctacgattt cagaacgtgc accgataaag cagttgtcgc
    19921 cgatgatggt tgggttcgct tgtaatggct ctaacacacc accgatgccc acgccaccag
    19981 aaaggtgaac atttttaccg atttgcgcac aagaacccac tgttacccaa gtgtccacca
    20041 ttgtgccttc gccaacgtat gcaccgatgt tcacgaaaga tggcattaac accacgtttt
    20101 tctcgatatg tgaaccttta cgcaccaccg cacccggcac ggcacgaatg ccatctgctt
    20161 tgaattgctc ttcggtgtag ttgccgtatt tggttggcac tttgtcgtag aatttggttt
    20221 ctgcaccgtc gatgatttca ttgtcggcaa tacggaatga aagtaatacc gcttttttca
    20281 cccattggtt tactacccac tcgccatctt ttttctcggc aacacgcagt gaaccgttgt
    20341 ctaatcctgc gatcacctct tctactgctg ctttggtttc agcatcaacg gttttcggag
    20401 taatttccgc acggcgttca aatgccgctt caataatagc ttgtaaagac attgttgtgt
    20461 tccttcaata tataaattaa aacagaaaat tcggctcact ttttaacata gaaaggcgtt
    20521 tatttccaca agaaaaaaca tatcgcagaa atttagttta taattccaag ccacttattt
    20581 gatacaagcg gtgatttttg tggaaaattt tgcaaatctg cttaccggta aaacgcgtga
    20641 gcatttagtg ccgttgccga acgctctttc ggataaacat taccttcagc ctcaggtggt
    20701 tgaggctttt ttgacgttgc agcatgctgc aaaacaagcc ggctttaatt tacagcctgc
    20761 cagtactttt cgtgattttg aacgccaaaa agggatttgg aatgcgaagt ttaggggtga
    20821 gcgtaaggtt cacgatgata acggggttgc gattgatatg gcggcgttat cggatcttga
    20881 aaagtgcaaa gcaatgttga gatggtctgc ggtgccgggg gcaagtcggc atcattgggg
    20941 aacggaaatt gatatttttg accctgattt attgccggaa aatacgcctt tattgcttga
    21001 gccgtgggaa tatttggaag gtggctattt tgccgattta accaagtggt tacacgttaa
    21061 tgcggaaaca tttggctttt atttcccgtt tgacggtaaa cattctcgag tgggctttga
    21121 gccgtggcac attagctatc gcccgatttc aaatgaatat gagcaacgct ttaacgatga
    21181 aatcttaaaa gctgcgtggc aagatgaaga agtggcgggt aaggaatgct tacttgaaca
    21241 ttttgaagaa ttactaaaat gatccaatta attaacgaaa cccaacaaca cgacaaattc
    21301 cgccaaattt gcgaaaaatg gcagctggtt gaagataaat ccagcccgtt agccttagtg
    21361 ctaacggagg aacgattgga actacgcaaa ttagacgaac ccaagctcgg ggcgattgca
    21421 gtgaattttg tggacggtac gatggcacat cggcgtaaat tcggtggtgg gcgaggtgaa
    21481 gccgttgcga aagcggtcgg cattaaaggg gattatttgc caactgtgat tgatgcgaca
    21541 gccggtttag ggcgagatgc ttttgtatta gcagcggttg gttgtaaagt gctgttagtg
    21601 gaacgcaatc caattgtcgc tgccttgttg gaagatggtt taaaccgagc ttatcaagat
    21661 gtggaaatcg gcagttttat gcaagaaaga atgatcttag ccgatgttcg agacatttct
    21721 cagctagatc cacaatccca aatggctgag gtggtgtatt tagatccgat gtatccgcac
    21781 aaacaaaaaa gtgcgttggt gaaaaaagaa atgcgggtgt ttcaacattt agttggggca
    21841 gatttagatt cggacagctt tttcttaccg gcaaaaggat tggcaaaaaa acgtgtggta
    21901 gtgaagcgtc cggactatgc cccgttttta gcggagcaaa agcctgattt ttcacacacc
    21961 accaaaaatc accgttttga tgtttatctt tctcacctga aaaatgatta aaggtgggtc
    22021 aggatcggtt tttaaaaaac tttaacttga aaagctgcct atgcgatagg caagcggtta
    22081 tttttacagt aaaatttaca aattatgtta tcgtttattt atatttaaaa aggcggttat
    22141 cgtgccacaa cgtgattatg tcgctcgttc caacgagaaa aaaagtaaaa aaaatcaaaa
    22201 agaaagtaat gttaaaaaga ttttaatgcg gattatcggg gtaattttat tcttcggtat
    22261 tggtttatgg tttttgaaat ccagtgcacc tgtaccaaca gttccgtcag caccgcagcc
    22321 tgctaattca cagactaaaa gcgaattacc aagtcgaccg gaagagacgt ggagctatat
    22381 tcgtgattta gaaacccgta cggtaattac cgataatagc caaacttcac aagaacgttt
    22441 ggcacaatta acggcggaac aaaagcagca aattgaagaa agacgcaaac aagaagaagc
    22501 acgcttagcc gctgagcaag ctcaacaaaa gcctgcgacc gatgtatctg ctacagaatt
    22561 ggctccgaaa acagaggaag ttaagtcgtt aacggaagaa gaattagcag caaaaaaagc
    22621 acaggagcaa gctttagcac aaaagcaagc ggaagagaaa cgcagacaag cagagcttaa
    22681 aaaacagcag gaagcgaaaa aagagcagcc ggctaaaatt gtagcaagtg ctgcaacact
    22741 ggcagataaa gctgcaaatc aggcgggtaa atttgggtta cagtgcggtg catttaaaaa
    22801 taaagcccaa gccgaaaata tgcaagcaag attggcaatg gcaggtttta acgcccgtat
    22861 cgcaagcagt gccgattgga accgtgtttt tgtcggcccg ataggtgaca gagcggcaac
    22921 ttccagagct caatccaatg cacgcagtgt agcggaatgt gtgattgtgg cgatgtaaaa
    22981 ttgattgaaa tattgaagag agctataggc tctctttttt tatatcccaa tcgcatattt
    23041 caaggcttgc tttttgatgg gtgacacttt atcagccaat actaaaccga cattgcgggc
    23101 aattttgagc ggtaataaat cggtcttaaa cgctttataa aacacgtcca tgcctgtttg
    23161 catcagtaaa ttatccggtt tacgtttttg ctcatagcgt tgaagcacct gctcacttgc
    23221 aaaatttttg ccttttttga ccgcttgttc gatcacctca agcaaggttt taacatcttt
    23281 aaagcctaaa tttacacctt gtccggcaag cggattaatg gtgtgagccg catcgccgac
    23341 taataccacc ccgttttgca catagtgctg agcgtgctgg cgggttagcg ggaaagcccc
    23401 tgaagcctgc acctgaatgt tgcctaattc tgcgttgagc cggcttggga aatgttgctc
    23461 aatcgcaact cccagctttt ctgctggaag cattttcagt tcggcaattt tttgtggcga
    23521 atcgtaccaa acaacgcagc cgtgattgcc ggaaagcggt aaaaaggctc gagggccggt
    23581 tggatagaat tgttgccaag tgccgcttgg agcaggtttt tcggtggtaa cggtggcgag
    23641 taaacagtgt tggcgatatt gccaagcggt taaaccaatg cccgcccatt ggcgaacctg
    23701 tgaatttgca ccgtctgcgg caatcagcag tggggcagca aa
//
LOCUS       DANKBV010000026        23692 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.026, whole genome shotgun sequence.
ACCESSION   DANKBV010000026 DANKBV010000000
VERSION     DANKBV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23692
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.026"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            9..1436
                     /gene="hldE"
                     /locus_tag="PWY43_000794"
     CDS             9..1436
                     /gene="hldE"
                     /locus_tag="PWY43_000794"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDL2135693.1"
                     /translation="MMMQYSPKFNNAKVLVLGDVMLDRYWFGATNRISPEAPVPVVKV
                     QDIEERAGGAANVAMNIASLDVPVTLHGLIGDDDAGRALDKLLNSHQIQNHCVAINSH
                     PTITKLRILSRHQQLLRLDFEEGFHNVDSTALLAKLSQEITAYGALILSDYGKGTLES
                     VQQMIQIARNAKVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGHCKDEDEIVSKGL
                     KLIADYDLSALLVTRSEKGMTLLRPNHEPYHLPTQAKEVYDVTGAGDTVISVLATAIA
                     DGRAYEEACYLANAAAGVVVGKLGTSTVSPVELENAIHQRTATGFGLVSEAELKAIVE
                     EAKKRGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNTDASVKRLKGEARPIN
                     DLAARMAVLAGLASVDWVVPFDEDTPQRLISEILPDLLVKGGDYKPEEIAGSQEVWAN
                     GGDVRVLNFENGCSTTNVIKKIQAL"
     gene            1450..2004
                     /gene="pyrR"
                     /locus_tag="PWY43_000795"
     CDS             1450..2004
                     /gene="pyrR"
                     /locus_tag="PWY43_000795"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL2135694.1"
                     /translation="MTREKIIIDTEQFQRTISRISHQIIEKHGDLQDVVIVGIKRRGA
                     EIAELIQKRIEDLAQTRLPYMALDITFYRDDLDLIYQDPVFMGADNQLNLNGKKVILV
                     DDVLFTGRTIRAALDALLDFGRAARIELVIFVDRGHRELPIRADYVGKNIPTAKNEQI
                     QVQTLSYDGINQVVLVPAANKESA"
     gene            2077..3030
                     /locus_tag="PWY43_000796"
     CDS             2077..3030
                     /locus_tag="PWY43_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2135695.1"
                     /translation="MKMKLVKSLFAVAVATMSLTQTAHAFEERVVALVNDTPIMQSQV
                     QRVLGKKKATETAQRAAIDQVIDDMLVQQAMKEAGVKVSPAAVNQAVENVAIQNGITY
                     GQLLDALDYQGITLEQFKRNIAQQMAMEQVRHISISKSIQVAPQQVQALAKELMAKDK
                     AAGKLKTVSGKEHRISHILLKTNPILNDAQAKAKLSSLTADINSGKISFEEAAKAHSV
                     DYASGTDGGDLGFNFLEIYDPAFANVASKIKPNQISAPFKSQFGWHILKVTDTRNGDR
                     TEDAYHQRAYQQLVNKQAEEASKDWVKALRKTANIQLSVNN"
     gene            3088..3954
                     /gene="rsmA"
                     /locus_tag="PWY43_000797"
     CDS             3088..3954
                     /gene="rsmA"
                     /locus_tag="PWY43_000797"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL2135696.1"
                     /translation="MSSTNSKKHLGHTARKRFGQNFLHDMNIIHSIVSAINPKNGQFL
                     LEIGPGLGALTEPVAELVDKLTVVEIDRDLAERLRHHPFLNQKLTIIEQDALRFNVRD
                     YFNQLNLDEDSVRVFGNLPYNISTPLIFHLLTFHDLIQDMHFMLQKEVVKRLCAAPNS
                     KAYGRLTIMAQYYCQVIPVLEVPPTAFKPAPKVDSAVVRLVPYNQLPYPAKDVYWLNR
                     VTTQAFNQRRKTLRNALSTLFSAEQLEALGVDLTARAENLTIADYVRLANWLHDNPPA
                     IDTTEELVDEDC"
     gene            complement(4065..4694)
                     /gene="upp"
                     /locus_tag="PWY43_000798"
     CDS             complement(4065..4694)
                     /gene="upp"
                     /locus_tag="PWY43_000798"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL2135697.1"
                     /translation="MKIVEVKHPLVKHKLGLMREAEISTKDFRELANEIGSLLTYEAT
                     KDLEMESVEINGWSGEKIAVERIKGKKVTVVPILRAGLGMMDGVLEHIPSARISVVGI
                     YRDEETLKPVPYFSKLANDVDERLAIITDPMLATGGSMVATIDLLKKAGCKQIKVLVL
                     VAAPEGIKVLEAAHPDVELYTASIDSHLNENGYIIPGLGDAGDKIFGTK"
     gene            complement(4719..6404)
                     /gene="fadD"
                     /locus_tag="PWY43_000799"
     CDS             complement(4719..6404)
                     /gene="fadD"
                     /locus_tag="PWY43_000799"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDL2135698.1"
                     /translation="MEKIWLNNYPPYSPKTIETDQYESLVEMFEAAIQRHPDIPAYIN
                     MGQVLTFRKLEERSRSFAAYLQNELRLKKGDRIALMMPNLLQYPIALFGALRAGLVVV
                     NVNPLYTPRELEHQLNDSGAKAIVIVSNFATTLEKIVFNTEIKHVILTRMGDQLSFGK
                     RTLVNFVVKYIKKLVPKYKLPHAVSFREALSIGKQRQYVKPNIVSQDLAFLQYTGGTT
                     GVAKGAMLTHQNLIANMMQAKWIVEPLLGNSANMIGLVPLPLYHVLALSMNCLLFIEL
                     GLTGLLITNPRDIPGFIKELKKYKVSVITGVNTLFNALLNHESFKEVDFSHLKLSVGG
                     GAAIQSSVAKRWHDLTGVHIIEGYGMTECSPLISATRGDSVEYSGSIGVPVPNTDIRI
                     VDDEGKEVPLGERGELVVKGPQVMQGYWNRPDETAQVLQDGWMATGDIVVMGDDLNLR
                     IVDRKKDMIIVSGFNVYPTEIEEVISQNPKVNEVVAIGVPSEASGESIKIFVTKKDES
                     LTRDELRTYCRQFLTGYKIPRDIEFRDELPKSNVGKILRRVLRDEELAKVNKA"
     gene            complement(6382..6846)
                     /locus_tag="PWY43_000800"
     CDS             complement(6382..6846)
                     /locus_tag="PWY43_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDL2135699.1"
                     /translation="MMIEQATVVEYQNGVAIVQCYAKSSCGGCAAQSACGSKALSALA
                     GEKIDPRFRIEVDEPLKVGDKVELGLAENILLKSVFLIYGIPLLVLVMTAVGFSQFFA
                     NELIVLFIMLLSTGITFWAVRKLIHKHSKQANFSPIFLGKVLGDYNGKNMAK"
     gene            complement(6843..7826)
                     /locus_tag="PWY43_000801"
     CDS             complement(6843..7826)
                     /locus_tag="PWY43_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HDL2135700.1"
                     /translation="MLFKIKKYLLLFWVISACYTFAWAESTIKTPLAYLTAMSEAHKK
                     STYELVYILQQGSDTESFRLRHAFTHNKEYAQLLNLDHSREEIILKNNKVSYLGYNFR
                     PFSLNSSQILDNLPNVLYTDYANLKGYAFLDAGKDRISDRITKVIRMKPNDTLRYAYI
                     LWIDEETHLLLKSQVLNMDNLVLEEFRVLQLYQSPELDRIANVIESLMLPTLTTVKKE
                     EFPKSDGWQVKWLPTGFRLIQQQTVTGATYQVESEYVDSRVYSDGLSTLTIYIMPSQG
                     VSFNEYAWQQGKLTILNQTINDRDIVIIGDVPLQSAKQIMNNIGFKEGAQP"
     gene            complement(7984..8607)
                     /locus_tag="PWY43_000802"
     CDS             complement(7984..8607)
                     /locus_tag="PWY43_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2135701.1"
                     /translation="MQERITQQNERLSAYMDGQNVDTAFVETLTNSPELQQKWASYHT
                     IRSVMQGDEIILGTDFSAKMEALLENEEIESQANVEKSKGLLLKLKRWGTPIMQAGIA
                     ASVCLVAVLGVNSMNASDEMAQTQQPVLQTLPFSNSVEAVSYNAPVQDQPTEEQLELQ
                     QRKINALLENHELQRRTNNVKNLTLSEEEKQKAQTSSNTESEPNIQK"
     gene            complement(8653..9225)
                     /gene="rpoE"
                     /locus_tag="PWY43_000803"
     CDS             complement(8653..9225)
                     /gene="rpoE"
                     /locus_tag="PWY43_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDL2135702.1"
                     /translation="MSEQITDQELVEKAQKGDKKAFNLLVVRYQNRVAGLLTRYVARD
                     DIPDIVQESFIKAYRSLESFRGESAFYTWLYRIAVNTAKNHLTSLGRRPPKEDILAED
                     AESYDVGAQLRESDTPENLILSEELKKVVFDTIENLPDELKTAIRLRELEGLSYEEIA
                     EVMECPVGTVRSRIFRAREAIDAKIKPLMQ"
     gene            complement(9447..10991)
                     /gene="leuA"
                     /locus_tag="PWY43_000804"
     CDS             complement(9447..10991)
                     /gene="leuA"
                     /locus_tag="PWY43_000804"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL2135703.1"
                     /translation="MSNNNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVM
                     EVGFPVSSAGDFESVQTIARHIKNSRVAALSRAVAKDIDAAYEALKVAEAFRIHTFIA
                     SSALHVEAKLKRSFDDVVEMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAI
                     NAGATTVNIPDTVGFCLPNEYGNIIHQVMNRVPNIDKAIVSVHCHNDLGMATANSLTA
                     VQNGARQIECTINGIGERAGNTALEEVVMAIKTRQEFMGVDTRINTQEIHRVSQMVSQ
                     LCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEIMSPETIGLKKEKLNLTARSG
                     RAAVKGHMADMGYTETDYDLDKLYEAFLQLADKKGQVFDYDLEALAFIDMQQGDEDRL
                     KLDVITTQLISGLPASAFVQVELDGVRQSATSQGGNGPVEATYNAIMNIVGMELKMSH
                     YNLTAKGEGAEALGQVDIVVEYEGRKFHGVGLATDIVESSARALIHAINAIYRSQKVA
                     DLKAKK"
     gene            11475..12290
                     /locus_tag="PWY43_000805"
     CDS             11475..12290
                     /locus_tag="PWY43_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2135704.1"
                     /translation="MTLPFRAIISDLDGTLLNANHKIGDFTIDTLEQLSKKGVDIFFA
                     TGRNHPDVSQIIGKVNVKNAMLVTSNGARANNLEGKKILNHYLPEDLAFELMNIEFDD
                     QNVCLNSYQGDEWFINKEIAELKKFHQDSGFSYQVVNFAHHHGRNTEKVFFIGKTAEA
                     LVPIEHYIRDTYGDRVQMTYSALLCLEVMAKGVCKANALAELVKLRGYTLADCIAFGD
                     GMNDVEMLSLAGKGCLMGNADPRLKAVLPNNEVIGFNKDEAVASYLRATFGIV"
     gene            12356..13519
                     /locus_tag="PWY43_000806"
     CDS             12356..13519
                     /locus_tag="PWY43_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL2135705.1"
                     /translation="MKRKTLATLVLIAALGIGGYFYYQQQTNKPQIHYITQAVTRAEI
                     NKNVLATGSVRANKRTEVGAQVSGKIQKLYVELGQSVKQGDLIADIDSSNQSNTLSTA
                     QARLSSYKAQLTSAQVALEVAQSNYNRLNKLYRANSASLNDLETAKNTLASAKATVDN
                     IKAQLKSAEISVNDAKTNLTYTQIISPMDGVIVSVPVSVGQTVNSNQTSPTIVQVADL
                     SKMLIKLEISEGDIAQVKVGQKVKFSTLAEPNREYSATIDSVDPALTTLTDNNYTEQS
                     GNTEAVYYYANVSVDNADNSLRIGMTTQAQITIDKREQVLVVPTSVIKKRGKENYVLV
                     LENEQSVERKIQIGISDTQNTEVLAGLNEGDQVIVTQRADDEKISTARGPRMF"
     gene            complement(13632..14576)
                     /locus_tag="PWY43_000807"
     CDS             complement(13632..14576)
                     /locus_tag="PWY43_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HDL2135706.1"
                     /translation="MQTLLKFTQFISKTFAIWVVFFAFIAAQFPETFKQFLPWIPYLL
                     GIVMLGMGLTLTFKDFAEVTKNPKSVIIGVIAQFVVMPSVAYGLAKAFNLPADLAIGV
                     ILVGCCPGGTSSNVMTYLARGNTALSVACTTISTLLAPVLTPAIFYLFASQWLEINAT
                     AMFISVLQMVLLPIFVGVVIRTIFKQKVEQFSQTMPLISVIAIVLIVTAVVSVSRDRI
                     IESGLLIFAVVALHNGLGYLIGYLASRLLKLPIADSKAVAIEVGMQNSGLGAALAALH
                     FKANPIIAVPSAVFSFWHNISGPILAMIFAKMKNDNEK"
     gene            14724..15545
                     /locus_tag="PWY43_000808"
     CDS             14724..15545
                     /locus_tag="PWY43_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDL2135707.1"
                     /translation="MKNIKLSAMSLTALFVLTACSHTHKNSDEHHQMVLQEQATLGIN
                     WMQQSGEYQALAHQAFNAAKVAFDQAKVKKGKKKAVVVDLDETMVDNSLYAGWKVKNH
                     KPFDGESWTRWVNARQTGAIAGAVEFNNYVNSHKGTMFYVSNRKDSSEKAGTIDDMNK
                     LGFSGVSEQTLLLKKDKSNKTPRFEEIEKQGYEIVLYIGDNLNDFGDATYRKSNAERR
                     AFVAENSQQFGKKFIMLPNPNYGDWEGGLDKDYYKGNAESRVKIRHNAIKAWDGK"
     gene            15684..16964
                     /gene="pepB"
                     /locus_tag="PWY43_000809"
     CDS             15684..16964
                     /gene="pepB"
                     /locus_tag="PWY43_000809"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDL2135708.1"
                     /translation="MTAMKISLSTQNAPEIWGKNAILSANDAGFTVHNPNNDLATIQK
                     AARKIKNQGISTVELTGDSWDLKSCWAFHQGFHSVKNQGSVQYPDLDEQQAEFDNRVK
                     CATFVRELINKPSDKLTPEKLAEQAVEFIQKMAKPNTVSYQIIRGEALQEQGYHGIWT
                     VGKGSANPPAFLVLDFNPTNDENAQVLACLVGKGITFDTGGYSLKPSDSMSTMRTDMG
                     GAALLTGSLAFAIARGLNKRVKLYLCCAENMVSHNAFKLGDIITYRNGVTVEVLNTDA
                     EGRLVLADGLIDACKQNPQFIIDAATLTGAAKVAVGNDYHSVLSMDDNLTEALFKSAK
                     TENEPFWRLPFEEFHRGQIASSFANIANIGSVPVGAGASTATAFLSYFVENYQQNWLH
                     IDASATFRKTASDLWAAGATGLGIRTVANLLLSK"
     gene            17015..17497
                     /gene="greB"
                     /locus_tag="PWY43_000810"
     CDS             17015..17497
                     /gene="greB"
                     /locus_tag="PWY43_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDL2135709.1"
                     /translation="MAKSNYITRSGWQALDQELKFLWREERPKVTQAVSDAAALGDRS
                     ENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEEGEAK
                     QYRIVGCDEFDPAKNWISIDSPVARALIGKQVDDEIRVETPLGKVLLYINKIWYEQAS
                     "
     gene            complement(17539..21063)
                     /gene="recB"
                     /locus_tag="PWY43_000811"
     CDS             complement(17539..21063)
                     /gene="recB"
                     /locus_tag="PWY43_000811"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDL2135710.1"
                     /translation="MKNLNPIQLPLNKSVLIEASAGTGKTFTIANLYLRLLLGIGCNP
                     LTVEQILVVTFTKAATEELKNRIRKNIQQCADFLKDQADGLEVESTKSYRNNLDFLAQ
                     IYPLIPNIHEALLRLSIAEREIDTASVFTIHGFCQKMLVQFAFESGVRFDLDLQPNQS
                     DLLKKLSEEVWREQFYPQDLAITYAVAEQLGTPEYALNAVRRYLSTELPEPNASLNQD
                     IAEHLAQYQQFINEVKSYWKTHSDELVETIRHDLKHKITLHGGWYRATTFEAGVEVMN
                     QWANSQHNHFPAGLYLFTTEKIAAGTKKSCEPIDSPHLAKNQAFLTAYKERFENSLSA
                     MLQYQFLIALKAKLAEYKQSHPQRSFDDLLTMLNQALKNLHTGEALAQKIRAMYPFAM
                     IDEFQDTDLTQYEIFSQIFMAENAENGFIMIGDPKQSIYKFRGADIFTYLRAAKQADE
                     QFTLGKNWRSLPEVVQAVNNLFSFANDNSPFLYEDIGFHPVEADSQKGQLVSEQGHFV
                     GYITENSKLDDLAEMCAYQIHQQLEAMAQGKFGVEKNGEFQSFAAKDIAILVRKGSQA
                     KRIKKALAKRNIKSVYLAELASVFGSEIATELCWLLQAALNPYHYKALLSALGSTLWG
                     LTAAEIHRYKENEQLWDLKVNQFVEYSNIWKEQGVLPMLHKIYLEQGIIERLKGLENA
                     DRLITDLLHLTEILQETSQALESEAELLRWYEKQVNSPDFNDEQQLRLESEEELIKIV
                     TIHGSKGLQYPIVWLPFVSQKSEGDTQTALSLYRNSQGALDWCLGSEEASFSLQYEAY
                     AEDLRLLYVALTRAESQLNLVLPKEISGWSPLHYLLADGEIPTGKVPVSDYFSQKRIA
                     ANLVEVAGELPFSLWEGEQSSDDYQAKTFSGNIRVVGQITSFTSLYAQHERFSEGKSE
                     RVMDLAQDRDVHIETIEAGYSSETAAFSPFQFPHGIKVGTVLHHFFEHCQFNEQIDRE
                     AVAKVCEQLGLSEEWIEPTALWFERILTTPLAEANFCLKAIDETKRLNEWQFYLRLKN
                     DKALHQLNALLKQHSPLAKTLPELQLPQLEGFVRGFVDCIVQVEEKFYLIDYKSNFLG
                     YLPQNYAKEHIQREMGRQRYDLQYLLYTLALHRYLTARLGEKYDYERDFGGVAYLFLR
                     AMNGENDNGVYFDKPTKQLIEEMDRLFG"
     gene            complement(21122..22513)
                     /locus_tag="PWY43_000812"
     CDS             complement(21122..22513)
                     /locus_tag="PWY43_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL2135711.1"
                     /translation="MNLRWQEYPENARKLFKLSLPIFISQLSASGMGLADIVMAGLVS
                     DDDVLAIAVSNSIYFPLFLFVLGLLNAITPTVSYLNGSSNRHLIANQVRQGYWIVFAM
                     SIPLMAIFLNSHLILDLMQTPAEFSIKSQQYLAVMAVGLIPALLAVSLRCMNDGLSNP
                     KPAMRITFFGLLLNIPLNYIFIFGKFGLPEMGAVGCGVATAIVNWIMFFMLLHYCYTN
                     SAQKDIRLFEKWIDAPSGQILLKLCKLGLPIAFAIFTEVMLFSTSSLFLSPLGSQVVA
                     SHQAALQTSSLFFMIPMSFGIATTIMIGQTLGQKNVEGAKILSYHALITATALAIIAA
                     IIIISLKNFIPFAFTNNPISIAITAHLLIFAAVYQIPDAIQAVANGILRGYKHTQPIL
                     YVTLFCYWGVSMPLGYILARTDWIVQPMAAQGFWLVFCIGLSLAGGLLLWQMRKIQAV
                     PKAQLIAKLERIK"
     gene            complement(22649..>23692)
                     /locus_tag="PWY43_000813"
     CDS             complement(22649..>23692)
                     /locus_tag="PWY43_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2135712.1"
                     /translation="TACNEEKAAEPKATAAEAKPQATNQTVHLYTWTEYVPEGLLDEF
                     TKETGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYFVSKMAKEGMLAELDHAQ
                     LPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNTADYKGETFTSWGDLWKPEFAG
                     KVQLLDDAREVFNIALLKLGKNPNTTNPDEIKEAFEELKKLRPNVLSFTSDNPANSFI
                     AGEVSVGQLWNGSVRIAKKENAPVDMVFPKEGPVLWVDTLAIPANAKNKENAHKLINY
                     LLSAKVAEKLTLAIGYPTSNVEALKVLPKEITEDTAIYPSAEVLQKSQWQDDVGDAIE
                     LYEKYYQELKAAK"
BASE COUNT         7098 a         5047 c         4570 g         6977 t
ORIGIN      
        1 tattaactat gatgatgcaa tactctccaa aatttaataa tgctaaagtg ctggtgcttg
       61 gcgatgtaat gttagaccgt tactggttcg gggcaaccaa tcgaatttcg cctgaagccc
      121 ctgtgccggt ggtgaaagta caagatattg aagagcgtgc cggcggtgcg gcaaacgtgg
      181 cgatgaacat cgccagtctt gacgtaccgg taacattaca cggcttaatt ggcgatgatg
      241 atgccgggcg tgctttagat aaattactta attcccacca gatccaaaat cattgtgtgg
      301 cgattaattc gcacccaacc atcacaaaat tgcggattct ttcccgccat cagcaattat
      361 tgcggttgga ttttgaagag ggttttcaca atgtagattc taccgcccta cttgcaaaac
      421 tttcgcaaga aattaccgct tacggagcat taattctctc ggattacggc aaaggcacat
      481 tggaatcggt gcaacaaatg atccaaattg cccgcaatgc taaggtgccg gtgctgatcg
      541 acccgaaagg cacagacttt gaacgctatc gtggggcaac attgcttaca ccaaatatgt
      601 cggaatttga agcggtggtt gggcattgta aagatgagga tgagattgtc agcaaagggt
      661 taaaactgat tgccgactat gatttatctg cactattagt tacccgttcg gaaaaaggaa
      721 tgaccttact tcgcccgaac cacgagcctt atcatctgcc gacccaagcc aaagaggttt
      781 atgatgtaac cggtgcaggc gataccgtaa tcagtgtatt agccactgcg attgccgatg
      841 gcagagccta tgaagaagcc tgctatttag ccaatgctgc cgccggtgta gtcgtaggta
      901 aattagggac ttcaaccgtc agcccggttg agctggaaaa tgcgattcac caacgcactg
      961 ccaccggttt tggcttggtt tctgaagccg aattaaaagc gattgtggaa gaagcgaaaa
     1021 aacgtggcga aaaaatcgtg atgacgaacg gttgctttga tattcttcac cccggtcacg
     1081 tttcctattt ggaaaacgcc cgtaaattag gcgaccgatt aattgtagcc gtcaataccg
     1141 atgcttcggt aaaacgccta aaaggcgaag ctcgcccgat taatgattta gctgcccgta
     1201 tggcagtttt agctggttta gcctctgtag attgggttgt gccttttgat gaagatacac
     1261 cacaacgttt aatcagtgaa atcctacctg atttattggt aaaaggtggt gattacaagc
     1321 cggaagagat tgccggcagc caagaagtat gggcaaacgg cggcgatgtg cgagtattga
     1381 actttgaaaa cggctgttct accactaatg tgatcaagaa aattcaagca ctataatggt
     1441 aataagcgaa tgacgagaga aaaaatcatt attgataccg agcaatttca gcgcacaata
     1501 tctcgtattt cacatcaaat tattgaaaaa cacggtgatt tgcaagatgt agtgattgtc
     1561 ggcatcaaac gtcgaggagc tgaaattgcc gagttaatcc aaaaacgcat tgaagattta
     1621 gctcaaacac gcttgcctta tatggcgtta gatattacgt tctaccgtga tgacctagac
     1681 ttaatttatc aagatcccgt atttatggga gccgataatc agctaaatct gaacggtaaa
     1741 aaagtgattt tggttgatga tgttctgttt accggcagaa ccattcgggc ggcacttgat
     1801 gcactcttag attttggcag agctgccaga attgaactgg tgatttttgt cgatagaggt
     1861 catcgggaat tgcctatccg agccgattat gtcggaaaaa atattccaac ggctaaaaac
     1921 gagcaaattc aggtgcaaac gctctcgtat gatgggatta accaagtggt cttagttcct
     1981 gctgccaata aagaaagtgc ttaaattaag tgaacctttt cgctaaactt atctctaagg
     2041 aaaagttact gcttaattac ctccattagg attgttataa aaatgaaatt agtcaaatca
     2101 ttatttgctg ttgccgttgc aacaatgagt cttacccaaa ccgcccacgc atttgaagaa
     2161 agagtggtgg cgttagtgaa tgatacccct attatgcaaa gccaggttca acgtgtgctt
     2221 ggcaagaaaa aggcgacaga aactgctcaa cgtgcggcta ttgatcaagt gattgatgat
     2281 atgttggtgc aacaggcaat gaaagaagcc ggcgttaaag tcagcccagc tgcggtaaac
     2341 caagcggttg aaaatgtggc aattcaaaac ggtattactt acggtcagtt attagatgcg
     2401 ttagattatc aaggtattac acttgaacaa ttcaaacgta atattgctca acaaatggca
     2461 atggaacaag ttcgccatat cagcattagc aaatcgattc aggttgcacc acagcaagtg
     2521 caagctctgg cgaaagagtt aatggcgaaa gataaagcag caggcaaatt gaaaaccgtt
     2581 tccggcaaag agcatcgaat tagtcatatt ttgctaaaaa ccaacccgat tttaaatgat
     2641 gctcaagcca aagcaaaact tagcagctta actgctgata ttaactcagg caaaatcagc
     2701 tttgaagaag cggcaaaagc ccattctgtt gattacgcct cagggacaga cggtggagat
     2761 ttaggtttta acttcttaga aatttacgat ccggcatttg cgaatgttgc atcaaaaatc
     2821 aaaccaaacc aaatttctgc tccgtttaaa tcgcaatttg gttggcatat tttaaaagtc
     2881 accgacactc gtaacggaga cagaaccgaa gatgcttacc accaacgtgc ttatcagcaa
     2941 ctggtgaaca agcaagccga agaagcctct aaagattggg taaaagcgct tagaaaaacc
     3001 gccaatattc aattgtcggt caataattaa aatttttaag aacctcgcat aatgcggggt
     3061 tcttttctta tttcaaaaag gataacaatg agttctacaa attccaaaaa acatttaggt
     3121 cataccgctc gtaagcgttt cgggcaaaac tttttgcacg atatgaacat tattcacagc
     3181 atagtttcag ccattaaccc gaaaaacgga caatttttat tagaaatcgg tccgggttta
     3241 ggagcattaa ccgagccggt tgccgaattg gtagataaac tcacggtggt tgaaattgac
     3301 cgtgatttag cagagcgttt acgccaccac ccgtttttaa accaaaaact caccattatt
     3361 gagcaagatg ccctacgctt taatgtccgt gactatttta accaactcaa cttagacgaa
     3421 gactcggtgc gtgtgtttgg taacttacct tacaacattt ctacgccctt gatattccac
     3481 ctgctgacct ttcacgactt aattcaagat atgcacttta tgttgcaaaa agaggtggtg
     3541 aaacgtttat gtgccgcgcc aaacagtaaa gcatacgggc gtttaaccat tatggctcaa
     3601 tactattgcc aagtaattcc ggtgctggaa gtgccgccaa ccgcctttaa accggcacca
     3661 aaagtcgatt ctgcggtagt gcgtttagtg ccttataacc aactgcctta tccggcaaaa
     3721 gatgtgtatt ggctcaatcg ggtcacgact caagccttta accaacgccg taaaacctta
     3781 cgcaatgctc tttcaaccct gtttagtgcg gagcaactgg aagccttagg cgttgattta
     3841 accgcccgag cggaaaacct taccattgcc gattatgtgc gtttggctaa ctggctacac
     3901 gacaacccgc cggcaattga tacaaccgaa gaattggttg atgaagattg ctaatcaata
     3961 aattacaagc ggtcattttt ctaccaagat ttgcaaatct ttagtgaaaa atggccgctt
     4021 gtttaaatca gctttacttg ttttgctgtt acttagtttt gatgttactt ggtgccgaaa
     4081 attttatccc ctgcatcgcc taagcctgga atgatatagc cgttttcgtt taagtgacta
     4141 tcgattgagg cggtgtagag ttcaacatct gggtgggcgg cttctaatac tttaatcccc
     4201 tctggagcgg caactaacac tagcacttta atctgcttac agcctgcttt tttcaggagg
     4261 tcaatggttg ctaccattga gccaccggtt gcaagcattg ggtcggtgat aatggctaaa
     4321 cgctcatcaa catcgttggc tagtttggag aaatacggca cgggctttaa ggtttcttcg
     4381 tcacgataaa tcccaaccac actaattctg gcacttggaa tatgctctaa cacaccgtcc
     4441 atcataccta agccagcacg cagaatcggc acaacagtca cttttttgcc tttaattcgc
     4501 tcaaccgcaa ttttctctcc gctccagcca ttgatctcca cactttccat ttccagatct
     4561 ttggttgctt cgtaagtaag cagactacca atttcattcg ctagctcacg gaaatctttg
     4621 gtgctgattt ccgcttcacg cattaagccc aatttatgct ttacaagcgg gtgttttact
     4681 tcaacaattt tcattttaag ctccttaatt agggtaattt aagccttatt cacctttgct
     4741 aattcttcgt ctcttaacac tcggcgaaga attttaccaa cattactttt tggtaattcg
     4801 tcacggaatt cgatgtctct tggaattttg tagccggtta agaattgacg acaataggtt
     4861 ctcaattcat ctcgggttaa actttcatct tttttggtta caaaaatttt aatgctttcg
     4921 ccggaagcct cactcggcac accaatcgcc actacttcat tcacttttgg gttttgggaa
     4981 atcacttctt caatttcggt tgggtacaca ttaaaaccgg agacaataat catatctttt
     5041 ttacgatcaa caatgcgtaa gttcagatca tcccccatta ccacaatgtc accggttgcc
     5101 atccaaccat cttgtagcac ttgtgccgtt tcatcaggac ggttccaata gccttgcatt
     5161 acttgcggcc ctttcaccac cagctcgccc cgttcgccga gtggtacttc tttgccttca
     5221 tcatccacaa tacgaatgtc agtatttggc actggcacgc caatagagcc ggaatattcc
     5281 accgaatcgc cacgggttgc actaatcagc ggagaacatt ctgtcattcc ataaccttcg
     5341 ataatatgta cgccggttaa atcgtgccag cgttttgcca cgctagattg gatcgctgca
     5401 ccgccaccta cacttaattt taggtgtgaa aaatcaacct ctttaaaact ttcgtgatta
     5461 agtaatgcat taaacaatgt atttacccca gtaatcactg aaactttgta cttttttaat
     5521 tctttaataa agcctggaat atcgcgcgga ttagtaatta ataatccggt aagtcctaat
     5581 tcgataaaga gcaagcaatt catcgaaagt gctaagacgt gatacagcgg taaaggtacc
     5641 aatccaatca tattggcaga attacccaac aatggctcaa caatccattt tgcctgcatc
     5701 atattagcaa ttaagttttg atgagtcagc attgcccctt tcgctacacc tgtagtaccg
     5761 ccagtgtatt gtaaaaaggc gagatcttgg ctgacaatat tcggttttac atattgacgc
     5821 tgtttgccaa tgcttaaggc ttcacggaag ctgaccgcat gtggcaattt atatttcggc
     5881 actaattttt tgatgtattt caccacaaaa ttgactaagg tgcgtttgcc gaatgaaagt
     5941 tggtcgccca ttcgagtcaa aataacgtgc ttaatctcag tattaaagac aattttttct
     6001 aaggtagtcg cgaagttcga gactatcaca atcgctttcg caccactgtc atttaattga
     6061 tgttctaatt cacgcggcgt ataaagcggg ttgacattca ccaccacaag cccggcacgt
     6121 aatgcgccaa ataaagcgat cggatattgc aataaattcg gcatcatcaa tgcgattcga
     6181 tcaccttttt tcaggcgtaa ttcattttgc aaataagcgg caaacgagcg gcttcgttct
     6241 tccaatttac ggaacgttaa aacctgcccc atattaatat aagctggaat atcagggtgg
     6301 cgttgtatcg ctgcttcaaa catttccact aaagattcat attgatctgt ttcaatcgtt
     6361 tttggtgaat aaggcggata attatttagc catatttttt ccattatagt ctcctaaaac
     6421 cttacctaaa aagataggtg aaaaatttgc ttgtttgctg tgtttatgaa tcaattttct
     6481 tacagcccag aatgttatac ctgttgaaag caacattata aataaaacaa taagctcatt
     6541 tgcaaaaaat tgcgaaaatc cgaccgctgt catcacaaga accagcagag gaatgccata
     6601 aattaaaaac acgcttttga gcaagatatt ttcagctaac cctagttcaa ccttatcgcc
     6661 tacttttagg ggttcatcta cctcaatacg aaaacgaggg tcaatttttt cacctgctaa
     6721 tgctgataat gctttgctgc cacaagcaga ctgagctgca cagccaccac agctactttt
     6781 agcataacat tgtacaatag ccacaccatt ctgatattcc actaccgtag cctgttcgat
     6841 catcatggct gagctccttc cttaaaacca atattgttca taatttgctt agccgattgc
     6901 aacggcacat caccaataat cacaatatct ctatcattaa tcgtttgatt taaaatcgtt
     6961 aatttacctt gttgccaagc gtactcatta aaactcacgc cttggctagg cataatataa
     7021 atagttaatg tagataaacc atcactatat actctgctat ctacatactc actttccact
     7081 tgatatgtcg cacctgtgac tgtttgttgc tgaattaacc taaaccctgt cggcaaccac
     7141 ttcacctgcc aaccatcaga cttaggaaat tcttcttttt tcactgtcgt taatgttggt
     7201 aacattagtg attctattac atttgcaatc ctatccaatt ctggagattg ataaagttgc
     7261 aaaactctga attcttctaa taccaaatta tccatattca agacttggct tttaagtaat
     7321 aaatgtgttt cttcatcaat ccatagtata taggcatatc ttaaagtatc atttggcttc
     7381 atacgaatta cctttgtaat tcggtcagaa atacgatctt taccagcatc taaaaaggca
     7441 taacctttta aatttgcata atctgtgtaa agtacattag ggaggttatc taaaatctgt
     7501 gaactattta aactaaaagg acgaaaatta taccccaaat agctgacctt gttatttttt
     7561 aaaataattt cttctcggct gtgatctaaa ttcagtaatt gggcatattc tttattatgg
     7621 gtaaaagcat gacgtaaacg gaaagattct gtatcacttc cctgttgaag aatataaact
     7681 aattcataag tggatttttt atgtgcttct gacatagccg ttaaatatgc taatggtgtt
     7741 ttaatagttg attctgccca agcaaatgta tagcaggcag aaataaccca aaacaacaat
     7801 aagtattttt ttatcttaaa aagcatattt attctcaagc caaattaact taggttattt
     7861 tagcaaaaaa ttttcactat ttcgctactt gttggttatt tcttattttt tcgctacatt
     7921 tacatacaaa aagccacata attatggata aattatgtgg ctttcagtaa cttattttac
     7981 ctattatttt tgtatatttg gctctgattc tgtattggaa gaagtttgtg ctttttgctt
     8041 ttcttcctca gataaggtta aatttttaac gttattagta cggcgttgta actcatggtt
     8101 ttctaataac gcattaatct tacgctgttg aagctctaac tgttcttctg ttggttgatc
     8161 ttgaactggc gcattataac ttaccgcttc aactgaattg ctaaatggca atgtttgtaa
     8221 tactggttgt tgcgtttgag ccatttcatc actcgcattc attgaattaa cccctaatac
     8281 cgcaactaag cacactgaag ctgcaatacc ggcttgcata attggagttc cccaacgctt
     8341 gagcttgagc aataagcctt tagatttctc tacatttgct tgagattcaa tttcttcatt
     8401 ttccagtaat gcttccattt tagcagagaa atctgtccct aaaatgatct catcaccttg
     8461 catcacacta cgaatagtgt gataacttgc ccatttttgc tgtaattcag ggctgttagt
     8521 taatgtttct acaaatgctg tatcaacatt ttgcccatcc atataagctg aaaggcgttc
     8581 attttgttga gtgatgcgtt cttgcataat tgactccaat gatttaaatc tcttctaaaa
     8641 taatctttct atctattgca taagtggctt gattttagca tcaatcgctt cccttgctcg
     8701 gaaaatacgg gaacgtacag taccaaccgg acattccatc acttctgcga tttcttcgta
     8761 acttagccct tccaattctc ttaaacgaat ggcggtttta agctcatcag gtaaattttc
     8821 aatcgtatca aacacgactt tctttaattc ctcggataat atcaaattct ctggcgtatc
     8881 actttcacga agttgcgcgc ctacatcata actttcagcg tcttcggcaa gaatatcttc
     8941 ttttggcggt cttctgccta aagaggttaa atgatttttc gcagtattca ctgcaatgcg
     9001 ataaagccac gtataaaaag cactttcgcc acggaaagac tctaaagaac gataggcttt
     9061 aataaaagat tcttgcacaa tgtccggaat atcatctctt gcaacataac gagtcagcaa
     9121 accggctact ctattttgat aacgtacaac aagtaaatta aatgcttttt tatcaccttt
     9181 ttgtgctttt tcgactaatt cttggtcggt gatctgttcg ctcaactgcg gactctccta
     9241 aatatcaata tgtcattttc cattgcacac tttgttagtg tgtgctttat gacaaaagtt
     9301 caaaaaattt gaaaaattgt gtaatttttt acattcctag ataaaaccta cttaataaac
     9361 taagcaagcg gtcggatttt agcgaaattt tgcaaaaatt tcaaaaaatc cgaccgcttg
     9421 tctatgttat gctttcgctc tataagttat ttcttcgctt ttaaatctgc cactttttgt
     9481 gaacggtaaa tcgcattaat ggcgtgaatt aaagctcggg ctgaagattc cacaatatcg
     9541 gttgccaaac ccacaccgtg gaattttcga ccttcatatt ccactacaat atccacttgc
     9601 cctaatgctt ctgctccttc gcctttcgca gtcagattat agtgcgacat ttttagctcc
     9661 attcccacaa tgttcataat ggcattgtaa gttgcctcaa ctggaccatt tcccccttga
     9721 gatgtagcac tttgacgaac gccgtctaat tccacctgta caaaagccga agccggtaaa
     9781 ccgctgatta gttgggtggt aattacatct aattttaagc ggtcttcatc gccttgctgc
     9841 atatcaatga aggcaagagc ttctaagtca taatcaaaca cttgcccttt tttatcagca
     9901 agttgcaaga aggcctcata aagtttatct aaatcgtaat ccgtttccgt ataacccata
     9961 tcagccatat gccctttcac cgctgctcta cctgaacggg cagttaaatt cagcttctct
    10021 tttttcaagc cgatggtttc cggcgacata atttcataag tatttttatt tttcagcata
    10081 ccgtcttggt gaatacctga agagtgagca aaggcattgc taccgacaat cgctttgttc
    10141 ggctgaatcg gcatattgca aagctggctg accatttggc tgacacggtg aatttcttgg
    10201 gtattaatgc gggtatcaac ccccataaat tcttggcggg ttttaattgc cattaccact
    10261 tcttcaagag cggtgttacc ggcacgttcg ccaataccgt tgatagtaca ctcaatctgt
    10321 ctcgctccgt tttgcaccgc tgtcaatgag ttggcggtag ccatacctaa atcgttatgg
    10381 cagtgaacag agacaatcgc tttatcgata ttcggaacac ggttcatcac ttggtggata
    10441 atattgccgt attcattcgg taaacagaaa ccgactgtat ccggaatatt cactgtggtt
    10501 gcaccggcat taatggcagc ttctacaatg cggcagatgt tatcaattcc agtacgccca
    10561 gcatcttcac aggaaaactc aacatcatca gtataatttc tggcgcgttt taccgctgca
    10621 accgccattt cgactacatc atcaaatgaa cgttttaatt tagcctcaac gtgtaatgcc
    10681 gagctggcaa taaaggtatg gatacggaat gcttctgcta cttttaaggc ttcataagct
    10741 gcatcaatgt cttttgcaac cgctcgggaa agcgccgcca cccggctgtt tttgatatga
    10801 cgagcgatgg tttgtactga ttcaaaatcg cccgctgaag aaactggaaa accgacttcc
    10861 atcacatcaa cccctaaacg ttctaaagca agggcaattt gcaatttttc ttttacggtt
    10921 aaacttgctt ttaatgattg ctcaccatca cgtaaagtcg tatcaaaaat aataatgtta
    10981 ttgttgctca tagaagcctc tttttaggtg tttttatttg ataaaaaaag cccgcatttt
    11041 aaggtgcggg caagaatgtg gatagtccat ttactttttc caatcaatct ttatcctcgc
    11101 acaaaatacg ataacagaga taatcggagt aaggtaaatt tttccatgtt gtgttgtgtg
    11161 tttatgtttg cattgttatt cagtagttga ggtgtatatt atccttatcc aaatcaatgt
    11221 caatagcatt tttctcttca cacaaccgtt taccagccca ttaattcaag tcataccaga
    11281 ttattatatt gaataaatcc aacttattag taaaaaaaac tatttcattt aagcaaaatg
    11341 atacaaagaa ttaattttaa taataaaaca aatatttaga atttaatgat atttttgtaa
    11401 aaaaatttgt tttctttgaa gtttcagatc aaatttggta gcctttgata ttattttatt
    11461 gttaggaata gattatgact ctcccatttc gtgcaattat ttccgatctt gatggcacac
    11521 tattaaatgc caatcataaa attggtgatt tcactattga tacattagaa caactatcaa
    11581 aaaaaggtgt tgatattttt ttcgccaccg gtcgcaatca tccagatgtg tctcagatta
    11641 ttgggaaagt aaacgttaaa aatgcaatgt tagtcacttc aaacggggca agagccaaca
    11701 atttagaggg taaaaaaatt ctcaaccatt atttaccgga agatcttgcc tttgaattaa
    11761 tgaacattga atttgacgac caaaatgtgt gcttaaacag ctatcaaggc gatgaatggt
    11821 ttatcaataa agagattgca gagttaaaaa aattccatca agattcagga tttagctatc
    11881 aagtagtaaa ttttgctcat catcacggtc gaaataccga gaaagtgttt tttatcggca
    11941 aaactgctga agctttagta ccgattgaac attatattcg ggatacctat ggcgaccgtg
    12001 tgcaaatgac ctactctgcc ctactttgcc ttgaagtgat ggcaaaagga gtgtgtaaag
    12061 ccaatgctct cgccgaattg gtcaaattac ggggctacac cttagccgat tgtatcgctt
    12121 ttggtgacgg tatgaatgat gtggaaatgc taagtttagc cggcaaaggt tgtttaatgg
    12181 gtaatgccga tccacgttta aaagccgtat taccgaataa tgaagtgatt ggctttaaca
    12241 aagatgaagc ggtggcaagc tatcttcgag ccacatttgg cattgtttag ttgagagcaa
    12301 cttttttgca aatttttgtc taaatttgac cgcttgtata tcatcaagga acagtatgaa
    12361 acgtaaaaca ttagccactc ttgtgctaat tgccgctctc ggaataggcg gctattttta
    12421 ttatcagcaa caaaccaata aaccgcaaat ccattacatt acccaagctg tcacacgggc
    12481 agaaattaac aaaaatgtgc tggcaaccgg ttcggttcga gcgaataaac gcaccgaagt
    12541 gggggctcaa gtttcgggca aaatccaaaa actctatgtc gagctcgggc aatcggtgaa
    12601 gcaaggagat ttaattgcag atattgattc cagcaaccag agcaacacct taagcaccgc
    12661 tcaagcccgg ctttcttctt ataaagcaca gctcacctct gcacaggttg cacttgaagt
    12721 agctcaatcg aactacaacc gcttaaataa actctatcgg gcaaatagtg cctctttaaa
    12781 cgatcttgaa acggctaaaa ataccttagc ttcagccaaa gccacggtgg ataacattaa
    12841 agcacaatta aaatcggctg aaatttcggt aaacgatgct aaaaccaact taacttatac
    12901 gcaaattatc tcgccaatgg acggcgtgat tgtgtctgta ccggtttctg tcgggcaaac
    12961 ggtcaattcc aatcaaactt cgccaactat tgtacaggta gcagatttat ctaagatgct
    13021 gattaaactt gaaatttcgg agggcgatat tgcacaggtt aaagtcgggc aaaaagttaa
    13081 gttttccacc cttgctgagc caaatcgaga atatagtgct accattgatt cagttgatcc
    13141 ggcactcacc acgctgaccg ataataacta caccgaacaa tcgggtaata ccgaagcggt
    13201 ttattactac gctaatgttt ccgttgataa tgccgataac agtttacgca tcggaatgac
    13261 tactcaagcc caaattacta ttgataaacg ggaacaggtt ttagttgtac ccaccagcgt
    13321 aatcaaaaaa cgtggtaaag agaattatgt gttggtgctg gaaaatgagc agtcggttga
    13381 aagaaaaatc caaattggca tttcggatac gcaaaatacc gaagtattag caggcttaaa
    13441 tgagggtgat caagtgattg ttacccaaag agcggatgat gaaaaaatca gcactgcacg
    13501 tggaccaaga atgttctaaa ttctaaacaa caagcggtta aattttctga acattttgca
    13561 aaaaattaga aaaatttaac cgtttgtcta ttctttatta aaacattaaa acgtctgtat
    13621 taaaacgaat tttatttttc gttatcgttt ttcattttag cgaaaatcat cgccaaaatc
    13681 gggcctgaaa tattgtgcca gaagctaaat accgcacttg gcaccgcaat gatagggttg
    13741 gctttaaagt gtaatgcggc aagtgctgcc cccaatcctg agttttgcat ccccacttca
    13801 atcgccaccg ctttgctgtc tgcaatcggt aattttaata aacggctcgc caaataaccg
    13861 attaaataac ctaaaccgtt gtgtaatgct actaccgcaa aaattaataa accggattca
    13921 ataatacgat ctcggctaac tgacaccacc gccgtgacaa ttaatacgat tgcaatcact
    13981 gaaattaatg gcattgtttg gctaaattgc tctacttttt gtttgaaaat agtacgaatc
    14041 accacgccca caaaaattgg caatagcacc atttgcaaca ccgaaataaa cattgccgtt
    14101 gcattaattt ctaaccattg gctggcaaat aaatagaaaa ttgccggcgt aagcactggg
    14161 gcaagtaaag ttgaaatagt ggtacaagct accgataacg ctgtgttgcc acgagccaaa
    14221 taggtcatca cgtttgaaga agtaccgccc gggcaacaac caactaaaat cacaccaatg
    14281 gctaaatctg cgggtaagtt aaatgctttt gccaatccat aagctacact cggcattacc
    14341 acgaattgag cgatcacgcc gataatcact gatttcggat ttttcgttac ctcggcaaaa
    14401 tctttgaaag ttaaggttaa ccccattccc aacatcacaa tccctaacag ataaggaatc
    14461 caaggcagga attgcttaaa ggtttccgga aattgtgctg cgataaaagc aaaaaacacc
    14521 acccaaatgg cgaaggtttt gctgataaat tgtgtaaatt ttaataatgt ttgcatatag
    14581 cctctgtgta attgttataa aaaggttact aggctacctt agtttgcttt aaaagtgaat
    14641 aggagcaata aactattagc cataagcaac tctttttagg tataatcgct ctgtttatca
    14701 acaataaaac taaggatata cgtatgaaaa acatcaaact ttctgctatg agcctcacag
    14761 ctttatttgt attaactgct tgttcgcaca cgcataaaaa cagcgatgag catcatcaaa
    14821 tggtgttgca agagcaagct acattaggga taaactggat gcaacaatcc ggcgaatacc
    14881 aagctcttgc ccatcaagca ttcaatgcag cgaaagtggc attcgaccaa gccaaagtga
    14941 aaaaaggcaa gaaaaaagcc gtggtagtag atttagatga aactatggtg gacaacagcc
    15001 tttatgccgg ctggaaagtt aaaaaccaca agccgtttga tggtgaaagc tggactcgtt
    15061 gggtaaatgc ccgccaaacc ggagcgattg ccggtgcagt ggaatttaac aactacgtta
    15121 atagccacaa aggaactatg ttctatgttt caaaccgtaa agacagcagt gaaaaagccg
    15181 gtactattga tgatatgaac aaattaggct ttagtggtgt gagcgaacaa acactcttgt
    15241 tgaaaaaaga caaatcgaac aaaacccctc gctttgaaga aatcgaaaaa caaggctatg
    15301 aaatcgtgct ctatattggc gacaacttaa acgatttcgg cgatgcgact taccgtaaat
    15361 caaacgcgga acgccgtgca ttcgtagccg aaaacagcca acaattcggt aaaaaattca
    15421 ttatgttacc gaacccgaac tatggcgact gggaaggcgg tttagataaa gattactaca
    15481 aaggcaatgc tgaaagccgt gttaaaatcc gccacaatgc aattaaagct tgggacggca
    15541 aataatccgt ccttaatcag gttgtaaaaa cgcgaaacaa tgaccgcttg tcaaggctat
    15601 gctctgcaag cggtcatttt tgtataaaac tttgcaaaag tttgttagga taaagcaaaa
    15661 tccattttct aaggaaaccg ataatgacag caatgaaaat ttcactttct actcaaaacg
    15721 cccctgaaat ttggggtaaa aatgctattc tttccgcaaa tgatgcgggg tttaccgtac
    15781 ataatccgaa caacgattta gccactattc aaaaagccgc ccgcaaaatt aaaaatcaag
    15841 gtatttcaac cgtggagctg accggcgata gttgggattt aaaatcctgc tgggcgtttc
    15901 atcaaggatt tcattcggta aaaaatcaag gctcggtaca atacccagac ttagacgaac
    15961 agcaagccga atttgataac cgtgtaaaat gtgccacttt cgtgcgtgaa ttaattaata
    16021 agccatcaga taaattaacc cctgaaaaat tagccgaaca agcggtcgag tttatccaaa
    16081 aaatggcaaa accgaatacg gtttcttacc agatcattcg tggcgaagca ttacaagaac
    16141 aaggctatca cggcatttgg acagttggaa aaggctctgc caatccgcct gcattcttag
    16201 tattagactt caacccaacc aacgatgaaa acgcacaggt actggcttgc ttggtcggca
    16261 aaggcattac ctttgacacc ggcggttata gtctaaaacc aagcgatagt atgagcacaa
    16321 tgcgaaccga tatgggcgga gcggcacttt taaccggctc acttgctttt gccattgctc
    16381 gaggcttgaa taaacgagtg aaactctacc tctgctgtgc ggaaaatatg gtcagccaca
    16441 atgcgttcaa attaggcgat attatcactt accgcaatgg cgtaactgtt gaagttttaa
    16501 acaccgatgc tgaagggcgt ttagtgcttg ccgatggttt aattgatgcg tgcaagcaaa
    16561 atccacaatt tattattgat gctgcaaccc tcacaggagc agcaaaagtc gcagtcggca
    16621 atgactacca ctcggtgctt tcaatggacg acaacctaac cgaagcacta tttaagtctg
    16681 ccaaaacgga aaatgaaccg ttctggcgtt taccgtttga agaatttcac cgtgggcaaa
    16741 ttgcttcttc ctttgcaaac atcgccaata tcggctctgt gccggtgggg gctggggcaa
    16801 gtaccgccac cgcattttta tcttactttg tagaaaacta ccagcaaaac tggctacata
    16861 ttgacgcctc tgccacattc cgcaaaacgg cgagcgattt atgggcggca ggagcaaccg
    16921 gactgggaat aagaacagta gcgaatttat tgctttcaaa atagaaactt cgtaggggca
    16981 aatcactatt tgctccataa acaattaaat aactatggct aaatcaaatt acattacccg
    17041 ttcaggctgg caagccttag atcaggaatt aaaatttcta tggcgggaag aacgcccgaa
    17101 agtgactcaa gcggtgtctg atgctgccgc attgggtgat cgaagcgaaa atgccgaata
    17161 tatttacggc aaacgccgtt tgagagaaat cgaccgccgt gtgcgttttt tatctaagcg
    17221 gctagaggtg ctacaaattg ttgattatca ccctaaacaa gaaggcaaag tctttttcgg
    17281 cgcttgggtg gaattagaaa atgaagaggg cgaggcaaaa cagtaccgta ttgttggttg
    17341 cgatgagttc gacccagcaa aaaactggat ttcgattgac tcccctgtcg cccgagcctt
    17401 aatcggcaaa caggttgatg atgaaattag ggtagaaact ccgcttggta aagtgttgct
    17461 ttatatcaat aagatttggt atgagcaagc gagttaatta cccataaagg ggcgaaaaat
    17521 tttcgcccct acacatattt acccaaacaa ccgatccatt tcttcaatca actgcttagt
    17581 cggcttgtca aaataaacgc cgttatcatt ttcgccgttc attgccctta agaacaagta
    17641 agccactccg ccgaagtctc gttcataatc gtatttttca cctaaacggg cagttagata
    17701 acggtgtaag gcgagagtgt aaagcagata ttgcaaatca tagcgttgtc tgcccatttc
    17761 ccgttgaatg tgttctttcg cataattttg tggcagatag cctaagaaat tcgatttgta
    17821 atcaattaag tagaatttct cctccacttg cacgatacaa tctacaaagc ctcgcacaaa
    17881 accttctaat tgcggtaatt gcaattcagg taaagttttt gctaaagggc tatgttgctt
    17941 taataacgca tttaattgat gtaaggcttt atcatttttt aagcgtaaat agaattgcca
    18001 ctcgtttaat cgtttggttt catcaatcgc ttttaagcag aaatttgcct ctgccaaagg
    18061 ggtagttaga atacgctcaa accataatgc tgttggttca atccattcct cacttagccc
    18121 taattgctcg catactttag caacagcctc tctatcaatt tgttcgttaa attggcaatg
    18181 ctcaaaaaag tgatgtaaca ccgtgcccac tttaataccg tgtgggaatt ggaatggcga
    18241 gaaagcggca gtttcacttg aataacctgc ctcaatggtt tctatatgta catctcgatc
    18301 ttgggctaaa tccattaccc tttctgattt cccttcacta aaacgctcgt gttgggcata
    18361 aagtgaagta aagctggtaa tttgaccaac cacacggata ttgccactaa aggtttttgc
    18421 ttggtaatcg tctgaagatt gttctccttc ccacagtgag aaaggtaatt ccccggctac
    18481 ttcaaccaaa ttagcagcaa tccgtttttg gctaaaataa tctgaaacag gcacttttcc
    18541 tgtaggtatt tccccgtctg ctaacaagta atggagtgga gaccagccgg aaatctcttt
    18601 cggcaacact aagttaagct gagattccgc gcgggttaat gccacataca gcaaacgtaa
    18661 atcttccgca taagcctcat actgtagcga aaaacttgcc tcttctgagc ctaaacacca
    18721 atccagcgca ccttgagaat tgcgatataa acttaatgct gtctgcgtgt ctccctcact
    18781 tttttgtgaa acaaaaggta accaaacaat tggatattgc aagcctttcg agccgtgaat
    18841 cgtcacaatt ttgattagct cctcttcact ttccaaccga agttgttgct cgtcattaaa
    18901 atcgggcgaa ttaacttgct tttcatacca ccgcaacagt tcagcttcag actctaacgc
    18961 ttgcgaggtt tcctgcaaaa tttcggttaa atgcaataaa tccgtaatca agcgatccgc
    19021 attttccaaa cctttcaaac gttcgataat gccctgctct aagtagattt tatgcaacat
    19081 cggcagaacg ccctgctctt tccaaatgtt gctgtattcc acaaactgat tgactttcag
    19141 atcccaaagt tgctcatttt ccttatagcg gtggatctct gccgccgtta agccccacaa
    19201 ggttgagcct aacgccgata ataatgcctt gtaatgatac ggattcagtg cggcttgcaa
    19261 taaccaacac aattctgttg caatttccga accaaataca gaagctaatt cagctaaata
    19321 gacggatttg atattacgtt ttgccaacgc ctttttgatt cgcttagctt gtgaaccttt
    19381 tcggactaaa atcgcaatat cttttgcagc aaaggattga aattcgccgt ttttttcaac
    19441 tccgaatttg ccttgagcca tcgcctcaag ctgttgatga atttgataag cacacatttc
    19501 agccaaatca tctaatttac tgttttccgt gatgtagcct acaaaatgcc cttgctcact
    19561 aactaactga cctttttggc tatcagcctc cactgggtga aagccaatat cctcataaag
    19621 aaacggagag ttatcatttg caaaagaaaa caaattattg accgcttgca ccacttcagg
    19681 taacgaacgc cagtttttgc ccaatgtgaa ttgttcatca gcctgctttg ctgctcgcaa
    19741 atacgtgaaa atatccgccc cacggaattt atagatcgac tgtttcgggt cgccaatcat
    19801 aataaagccg ttctctgcat tctctgccat aaaaatttgg ctgaaaattt catattgggt
    19861 taaatcggta tcttggaact catcgatcat cgcaaaagga tacatcgccc gaattttctg
    19921 agccaaggct tcgcccgtgt gtagattttt caacgcttgg tttagcattg tgagtaaatc
    19981 atcaaaactg cgttgtgggt gcgattgttt gtactctgcc aattttgctt tgagagcaat
    20041 taaaaattga tactgcaaca ttgctgacaa gctgttttca aaacgttcct tgtaagcggt
    20101 caaaaaagcc tgattttttg caagatgagg ggaatctatc ggctcacagc tctttttcgt
    20161 gccagccgca attttctctg tagtgaataa ataaagccct gccgggaaat ggttatgttg
    20221 cgaattagcc cactggttca tcacttccac accggcttca aaagtggtcg ctcgatacca
    20281 acctccgtgc agcgtaattt tatgctttaa atcgtggcgg atagtttcaa ccagttcatc
    20341 tgaatgcgtt ttccaatagg atttcacctc attaataaac tgctgatatt gagccaaatg
    20401 ctcagcaata tcttgattta aagaggcatt tggttcaggt aattcagtag ataaataacg
    20461 ccttaccgca ttgagggcat attccggcgt accaagttgt tcggcaaccg cataagtgat
    20521 ggctaaatct tgcggataga attgctctcg ccagacttct tcacttaatt ttttaagtag
    20581 atcactttgg tttggctgta aatccaagtc aaaccgcacg cccgactcaa acgcaaactg
    20641 aaccagcatt ttttgacaaa agccgtgaat ggtaaacacg ctggcggtat caatttcccg
    20701 ttcggcaata cttaaacgta acaatgcttc gtgaatattc ggaatgagcg gataaatctg
    20761 tgccaaaaaa tcaaggttgt ttcgatagga ttttgtactt tccacttcca aaccatcagc
    20821 ttgatctttt aaaaagtcgg cacattgctg aatattttta cggatacggt ttttcaactc
    20881 ttcggttgct gctttggtaa atgtcaccac caaaatttgt tccaccgtta acgggttgca
    20941 accaatgccg agcaataaac gcaaatacag gttggcaatg gtaaaggttt tccctgtacc
    21001 tgccgatgct tcgattaaaa cggatttatt caaaggtagt tggataggat ttaggttttt
    21061 catcagcaaa gttatcttct aaaagtaaaa agcactgcta tgttccatag cagagaatct
    21121 gttatttaat ccgttctaat tttgcaatta attgtgcttt tggcaccgct tgtatttttc
    21181 gcatttgcca taataacaag ccaccagcca aacttaagcc gatacaaaag actaaccaaa
    21241 aaccttgagc agccattggt tgtacaatcc aatcggttct tgccaaaatg tagcccagcg
    21301 gcatactcac cccccaataa caaaacagag taacgtataa aatcggttgg gtatgtttat
    21361 aaccacgcag aatgccgttt gctaccgctt gaatggcatc cggtatttga taaaccgccg
    21421 caaaaatcaa taaatgagcg gtaatcgcaa tcgaaatcgg gttgttggta aacgcaaaag
    21481 ggataaaatt tttcaacgaa ataataatga tggcggcaat aatagctaaa gcggttgcgg
    21541 taatcagtgc gtggtaactt aagattttcg ccccctccac atttttctgc ccaagtgttt
    21601 gtccgatcat aatggtggtt gcaatgccaa acgacatcgg aatcataaaa aatagcgaac
    21661 tggtttgcaa tgcagcttgg tggctagcca ccacctgtga accaagtggt gacagaaaca
    21721 gcgatgaggt cgaaaacagc atcacttcgg taaaaatcgc aaaggcaatc ggcagcccta
    21781 atttgcaaag ttttagcaaa atttgaccgc ttggagcatc aatccatttc tcaaacaaac
    21841 gaatatcttt ttgggcagaa ttggtgtagc aataatgcaa caacataaaa aacataatcc
    21901 agttcacaat tgcggttgct acaccacagc ctaccgctcc catttcaggc aagccaaatt
    21961 tgccgaaaat gaaaatatag tttagcggaa tgttcagcaa taaaccgaaa aaggtaatcc
    22021 gcatcgcagg tttagggtta gataatccat cgttcataca acgtaaactc accgccagca
    22081 aagcaggaat aagcccaact gccattactg ctaaatattg ttgcgatttg attgaaaact
    22141 cagccggtgt ttgcattaag tctaaaatca gatggctgtt taggaaaatt gccattaatg
    22201 gaatagacat tgcaaacaca atccaatagc cctgccttac ttggttagca attaaatggc
    22261 gattgctcga gccgtttaag taagaaacgg ttggggtaat tgcattcagc aaccctaata
    22321 caaacagaaa gagcggaaag taaatcgaat tacttaccgc aatcgccaag acatcatcat
    22381 cactcaccaa gccagccatt acaatatccg ccagccccat tccggaagct gaaagctggg
    22441 aaataaaaat gggtagggat agtttaaata atttgcgggc attttcagga tattcttgcc
    22501 atcttaagtt catggtttaa tctctcaaaa tctaagtatg tttttatgat tgtaccttag
    22561 aaagcggtcg tttttgccaa attttttgca actcaaaaga gaaagcctct aaagattgct
    22621 ccctagaggc ttcagaatta atcagctatt attttgctgc ttttaactct tggtagtatt
    22681 tttcgtaaag ctcaattgca tcaccaacat catcttgcca ttgagatttt tgtaaaacct
    22741 ctgcagatgg gtaaattgct gtatcttcag taatttcttt cggtaatact tttaatgctt
    22801 ctacgtttga agttgggtaa ccaatcgcta acgttaattt ttcagctact tttgcactta
    22861 ataagtagtt gattaattta tgtgcatttt ctttgttttt cgcatttgcc ggaatggcta
    22921 aagtatcgac ccaaagcaca ggaccttctt tcgggaatac catatcaacc ggtgcgtttt
    22981 ctttcttcgc aatacgtaca gaaccgttcc ataactgacc tacagaaacc tcaccagcaa
    23041 taaatgagtt tgccgggtta tcagaggtaa atgaaagcac atttggacgt aattttttca
    23101 actcttcaaa ggcttctttg atttcatccg gattagttgt atttgggttt ttacctaatt
    23161 ttaataacgc aatgttgaat acttcacgag catcatctaa taattgtact ttacctgcaa
    23221 attcaggttt ccacaaatca ccccaagaag tgaatgtttc acctttgtaa tctgccgtat
    23281 taaatgcaat acccggcgca cctaataatt gaggtaatga atatttgttt ccttgatcgt
    23341 aaggtttatt taaccaatct tggtttaatt ctttaataac cggaagttgt gcgtgatcta
    23401 attccgctaa cattccttct ttcgccattt tagatacaaa gtagtttgat ggagcaatta
    23461 catcataacc gccatctttg ccttgtaatt ttaatttagc gtacattgtt tcgttagatt
    23521 caagacttga aacttcaact ttaatgccgg tttctttcgt aaattcatct aacaaacctt
    23581 caggcacata ttcagtccaa gtataaaggt gaactgtttg atttgttgct tgaggttttg
    23641 cttcagccgc agttgctttt ggctctgccg ctttttcttc attacacgct gt
//
LOCUS       DANKBV010000027        23411 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.027, whole genome shotgun sequence.
ACCESSION   DANKBV010000027 DANKBV010000000
VERSION     DANKBV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23411
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.027"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            216..1982
                     /gene="aspS"
                     /locus_tag="PWY43_000814"
     CDS             216..1982
                     /gene="aspS"
                     /locus_tag="PWY43_000814"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL2135713.1"
                     /translation="MMRSHYCGALNRSHLGQTVTLSGWVHRVRNLGRFIFMQIRDREG
                     IVQVFFDEKDEALFKQASALRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNLVV
                     YNNAEVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRFMDDNGFLD
                     IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFMTAEEVRALMEAMIHGLWKKTLGVDLGKFPMMTWNEA
                     MTRFGSDKPDLRNPLEMVDVADILKSVDFKVFSGPANDPKGRVVALRVPNGAALTRKN
                     IDDYTQFVGIYGAKGLAWLKVNDMNTGLEGIQSPVAKFLNAEVLKALVKRLNLQKDDI
                     VFFGADNWHVATNAIGALRLKVGNDLGLTDTTAWKPLWVIDFPMFERDDEGNLSAMHH
                     PFTSPKELSPEELVQDPENAVANAYDMVINGYEVGGGSVRIFSQQMQQTVFSILGINE
                     EEQKEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTDNIRDVIAFPKTTAAACLMT
                     EAPSYANPKALEELAIRTIPQE"
     gene            2302..6666
                     /locus_tag="PWY43_000815"
     CDS             2302..6666
                     /locus_tag="PWY43_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL2135714.1"
                     /translation="MKHTFKLSLLATSIAISLSPTLLASIVRGDVDYQYFRDLAENKG
                     QFFVGATNIPVIDKTGKNIGTFLQVTPTKQVEVESNISPKPNVSLSECFDNESLPGCY
                     EFLYGGGDEDSNDLPVFETVTGETMSIPMIDFSSITRQTGIATLVNPQYVISVNHNQG
                     YRDIQFGDSSSEKADDHHYNYSVVRRNNYMPDPNKERTDLGKGIEKRERNDEGEKLIL
                     DGSGNPTPRWDYHAPRLSKLVTEVAPANEIERTTENVQDFYSVFSDPFIFPMFIRAGS
                     GRQAVINNERDRLNNGRIEVGNGPFLTGGSVLPVTNGDPSVPGSDFNSRILVAKTENN
                     TINDVFKDHGYGPLTTLGLPGDSGSALFGYDVRTKKWVVLGVYSDYFSENNTPGGDVY
                     KSYWNYHHPHYVRALEKENNAGAINANGARLTWTPSGNTSSIVGGNAPLTVNLADTSL
                     PTLQGHNGNTGDNPWNPQLQHGKTFHILGENNTLSLTENINQGAGAIHFHGNTTVEGM
                     KAGITWLGAGVDIDKDKNVIWKISNPAGDRLSKIGQGTLTINGKGENKGSVSVGDGTV
                     ILNQQADENNKKSAFSELGIVSGRPTVILNSSDQMDPNNIYFGYRGGRLDVNGNDLTF
                     RRIQNSDEGAMVVNHNTAQTANITLTGIVSPTIDQVKVQAITSPDQVSRHLDKDIFTY
                     QRFPHLPTNFIRLKKPLSVAEITAIFNARKVFIDLDFATGLIRENEYFEVLKVTDESA
                     AKQQILAEKIKAAEGTVQGFNGYFGETDPSKTNGRLNITMNSNEVANGKLLITGGTNL
                     NGTLSAEGTSEIILSGRPTPHAYDKVANKEVLIEGDWINRSFNATIFAAKDNGKLEIS
                     RNVANINGNFNLTDNATAQIGFTQGTSQACIRSDRTGVATCNINATLSETDLNSWERT
                     KVAGNVSLADNSTFSLGSKADLTGSITAQESTKVQLNDGSIANLTGLSTTGIFNGANG
                     STVNLSGVWTTLTNTVVDVLNVANGSQIHLNTPTATKQNTLTANTLTGNGIFKFVADL
                     TKTASDIVTIKDTASGQFSIEISTNGSAHSAQKIKLFETTNEEDFSLNLANEVRSGEY
                     RYHLLKEGNVYYLNPEKEEEPKITQKGPSETADPLPEYTGDIEYSEKGPGETADPLPE
                     YTGDIEHSEKGSGETAEPLPKYNGNAHLIASELTAQTNAVLGVTTVLDHVIHSEGTQN
                     WKVWSKTDYQTTEHRSAGYGFKQDANVIHLGLEKALDNQVTLGMILSQSKARNNFDHY
                     YSGKGRLTMLSMYAKKTWQNGVFVAIDTGFGKASNRLTYQANTVKLDRSVFVTGLSIG
                     KAWESANVNIIPSFSARYHHLSSAGNQLVDAKIETNAVDLLALQAGLSINKTLELNGL
                     QIKPEIGSYFVDASHGKLRTRFNNLQIEQQMGRYFKQEAGISANYRNINAGIQAGFLT
                     GNTLNKQRYISFKVTYEW"
     gene            complement(6741..7577)
                     /locus_tag="PWY43_000816"
     CDS             complement(6741..7577)
                     /locus_tag="PWY43_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL2135715.1"
                     /translation="MLPHIYIGTGGYADTDLIGTLYPFGTAKEDFLFVYSQHYDTLEI
                     NSTFHAPIGQKAFEGMVQKAEGRLNFSVKLHQDFSHQRTATLDQAQLFLNALAPFTAE
                     GLLANLFVQFPTQFERTPLNRRYLAELCQWFADYPLAIEFRHPSWHIPAVFDTFAKSS
                     NLIWCNVDYPQNIGLPAFHFFANQRTAYLRLHGRNPNWWKGQSAAECHDYRYSDEELK
                     GLAELLFNRRTEFDRLYLYFENTTKSHAFYNIQTLKGFLAEKGFQIKPVPENLLGQQS
                     LF"
     gene            7685..8560
                     /locus_tag="PWY43_000817"
     CDS             7685..8560
                     /locus_tag="PWY43_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL2135716.1"
                     /translation="MWIWFTLLAAVAQAGRNAFQKQLSVNVPVLGVTLARFFYAVPLA
                     SGYLYFLYQYDDNLNIPHFPPLFFVYAIGAGLAQILATSLMVQLFHLKNYAIGVGLAK
                     SEAVWAALLGVLFFGVTISGIGWLGVVLGGVAIFLMTGSGNLRQLSWKTLFLGIASGL
                     CFALTSLLVREASLQLNSHYLLSAAWVLFSVISFEAVILVIYLALLQKETLIKFFRFP
                     KLGMLTSLFSFLGSFGWFNAMSLNHVAFVKTLGQVEIFFILGISYFVFKERLKIQDFI
                     GLILVVIAAILVVLG"
     gene            8696..9136
                     /gene="nudB"
                     /locus_tag="PWY43_000818"
     CDS             8696..9136
                     /gene="nudB"
                     /locus_tag="PWY43_000818"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDL2135717.1"
                     /translation="MNYKNPHSILVVIYAKNTQRVLMLQRKDDPDFWQSVTGSINIGE
                     TPLEAAEREVSEETGLQTFEQKQPLVDCQKQIEFEIFPHFRYKYAPNITHCVEHWFLL
                     PLDSEQEPILTEHLAYRWVSVEDAVRLTKSPNNAQAIQEYLTSK"
     gene            9149..9889
                     /locus_tag="PWY43_000819"
     CDS             9149..9889
                     /locus_tag="PWY43_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2135718.1"
                     /translation="MAGHSKWANIKHRKAAQDAQRGKIFTKLIRELVTAAKLGGGDTS
                     SNPRLRAAVDKALSSNMTRDTINRAIERGVGGGDDTNMETKIYEGYGPGGTAVMVECL
                     SDNANRTISQVRPSFTKCGGNLGTEGSVGYLFSKKGLILVANADEDALTEAAIEAGAD
                     DIQPQEDGSFEIYTAWEDLGSVKDGIEAAGFKIENAEVTMIPSTTVELDAETAPKLLD
                     LINRLEDCDDVQNVYHNGEISDEVAALL"
     gene            10019..10546
                     /locus_tag="PWY43_000820"
     CDS             10019..10546
                     /locus_tag="PWY43_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135719.1"
                     /translation="MKLVKFVPALAATLVLAACSNANTQSAPQQAQDAVTKAAEQATQ
                     QVNEIAGNAPRASGAVKVEKATDGAKSIVYRCQSGKRVTANYVFQGEEAKAVNLLVQS
                     GGKTQKIPTLTRDASNQDSTSFKTDKYSWDLDTGFTLSSNGQSGMLTKYGAKTDTIVA
                     KLCDVDKALTSKLNK"
     gene            10613..11191
                     /gene="ruvC"
                     /locus_tag="PWY43_000821"
     CDS             10613..11191
                     /gene="ruvC"
                     /locus_tag="PWY43_000821"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDL2135720.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQNGRQLEYLGSGAIRTTAEDLPTR
                     LKRIYAGVSEIITQFQPNMFAIEQVFMAKNPDSALKLGQARGTAIVAAVNHDLPVFEY
                     AARLVKQTVTGIGSADKVQVQDMVTRMLKLSAKPQADAADALAIAITHAHSIQHSLIV
                     AKQSQQNVSSEKEQILALMKTRYSRGRFRLKG"
     gene            complement(11257..12537)
                     /gene="murA"
                     /locus_tag="PWY43_000822"
     CDS             complement(11257..12537)
                     /gene="murA"
                     /locus_tag="PWY43_000822"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2135721.1"
                     /translation="MEKFRVYGPFSLNGTVDISGAKNAALPILFAAILAEKTVTLKNV
                     PDLKDVDTTFKILRQLGVTVEKGSDKGVVHIDASKINNYVAPYELVKTMRASIWALAP
                     LVARFQLGQVSLPGGCTIGARPVDMHISGLEKMGAKIELDEGYVKATSNGRLNGARIY
                     MDKVSVGATLSVMMAGTLAKGTTVIENAAREPEIVDTADFLNAMGAKISGAGTDMITI
                     EGVEHLGGCEHSVVPDRIETGTFLVAAAISGGRITCRNTKADTLDAVIEKLREAGMQV
                     DVTENTITLDSLGRRPKAVNIRTMPHPGFPTDMQAQFTLLNTVAEGTSRITETIFENR
                     FMHIPELIRMGAKAEIEGNTAICYGVEQLKSAEVMATDLRASISLVLAGCIATGETIV
                     DRIYHIDRGYEHIEEKLRQIGARIERFSAPFEGE"
     gene            complement(12647..12907)
                     /locus_tag="PWY43_000823"
     CDS             complement(12647..12907)
                     /locus_tag="PWY43_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL2135722.1"
                     /translation="MNPSQIEEILKQALSDATEVHAQGENAHFGVIVVSDSIASLSKV
                     KQQQAVYAPLAEYFTTNAIHALTIKVFSVEKWKTERLLNMVG"
     gene            complement(12940..13263)
                     /locus_tag="PWY43_000824"
     CDS             complement(12940..13263)
                     /locus_tag="PWY43_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDL2135723.1"
                     /translation="MPQKTLQWDIQQNNESLFVKLSGELTRNTLLPLWKQRASFLSPT
                     GKQSLYWDLKEITRIDSAGFTLLAELLNHYHKIIPNSLINTPDSVRTLAALYDLDGWF
                     EQFII"
     gene            complement(13275..13910)
                     /gene="mlaC"
                     /locus_tag="PWY43_000825"
     CDS             complement(13275..13910)
                     /gene="mlaC"
                     /locus_tag="PWY43_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDL2135724.1"
                     /translation="MLKNIKKIIVTGLVAVSALFSTTAFAETSPYVLMQQTADKLFGD
                     IKANQAKIKSNPEYLRTIVRNDLMPHVHVKYAGQLVLGKNLSAATDAQKEAFFNAFGQ
                     FVEQSYAQVLTQYQDQSVQIESPKSVDGKSIVSIRVNVSGKGAAQPIKLDFKWRKNSK
                     TGEWQAYDMAAEGVSMVATKQNEWAGVIRQKGIDALIAQVAQSAKQPITLK"
     gene            complement(13956..14480)
                     /gene="mlaD"
                     /locus_tag="PWY43_000826"
     CDS             complement(13956..14480)
                     /gene="mlaD"
                     /locus_tag="PWY43_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDL2135725.1"
                     /translation="MRQSIKYEFWVGLFVLLGLAALVFLGLRVANVQGFSNEKTYTLY
                     ATFDNIGGLKVRAPIKVGGVVVGRVADISLDNKTYTPKVALAVNESFNQIPDTSSLSI
                     KTSGLLGEQYVALNVGFVIEGETAMMKEGDTFVDTNSAMVLEDLIGQFLYGDKKSDKT
                     EDEKASDSAEPKAE"
     gene            complement(14501..15277)
                     /gene="mlaE"
                     /locus_tag="PWY43_000827"
     CDS             complement(14501..15277)
                     /gene="mlaE"
                     /locus_tag="PWY43_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDL2135726.1"
                     /translation="MVNFISSIGAVVINFIRTLGRSAFMLWGALIGKPEFRKHTPLLI
                     KQLYVLGVQSLLIIMLSGLFIGMVLGLQGYVVLVDFAAESSLGTLVSLSLLRELGPVV
                     TALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLRRIIAPRFWAGVISMPVLAAI
                     FTAIGIWGGSLVGVDWKGVDGGSFWSVMQNSVNTSDLINGFIKSVIFAFAVVWIALFN
                     GYDCVPTSEGISQATTRTVVNASLVILGLDFILTAIMFGG"
     gene            complement(15270..16073)
                     /gene="mlaF"
                     /locus_tag="PWY43_000828"
     CDS             complement(15270..16073)
                     /gene="mlaF"
                     /locus_tag="PWY43_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDL2135727.1"
                     /translation="MTKTENLVEVKNLTFKRGERVIYDNLNLQVQKGKVTAIMGSSGI
                     GKTTLLRLIGGQILPESGEILFDGRDICKANNKELYEIRQRMGMLFQSGALFTDISTF
                     DNVAFPIRERTRLPEELIRKLVLLKLEAVGLRGAANLMPSELSGGMARRAALARAIAL
                     DPDLIMYDEPFTGQDPISMGVIVELIKELNQALNLTSIVVSHDVKEVLSIADYAYIVA
                     DKRVIAEGTPEILQASEDPQVVQFLAGKADGPVRFHYPAKDYTEELFNG"
     gene            16298..17692
                     /locus_tag="PWY43_000829"
     CDS             16298..17692
                     /locus_tag="PWY43_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HDL2135728.1"
                     /translation="MNKTMKKSFLTAFILGSTIAAMPAYAEATLPTAVNGQAVPSLAP
                     MLEKVRPAVVSIAVEGKTKGDSRRSREIPEEFEFFFGPHADLFGNRSSAPRNFRGVGS
                     GAVINAEKGYVITNNHVIDNADKITVKLEDGREFKAKLVGADPLSDVALIQVEDPKNL
                     TEIKFADSDKLQVGDFTVAIGNPFGLGQTVTSGIISALGRSTGQSDEGYENYIQTDAA
                     VNQGNSGGPLINLNGELIGINTAIISPSGGNAGIAFAIPSNMANSLVTQIIEFGEVKR
                     GMLGIKGGELNADLAKEFNISTQQGAFVSEVLPDSAAAKAGFKAGDVITEINGQKIRS
                     FSELRAKVATSGVGKEIELTYLRDGKEAKAKVTLQSDSEAKVTASNLIPALKGADFNN
                     YNAKGIKGVEISNVEKGSIAEMRSLKKGDIIIGVNRHSVENIADLRKILETKPSVVAL
                     NIVRDGANFYVILQ"
     gene            complement(17744..18505)
                     /locus_tag="PWY43_000830"
     CDS             complement(17744..18505)
                     /locus_tag="PWY43_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDL2135729.1"
                     /translation="MKTREQLSQENYQQAVKFAQHFADDVRQEFEREAEQRYQFERTQ
                     FEQSVLQNNAQNRPLAKIASLSCVAILLLAMTYYGLSGRYQVVQSGLQQHQDFQQQMS
                     DAHGTSKNERYILSLQNQLRENPNNGDLWYELGQAYALSNDFDSALVCYDNAEKLLGK
                     RAAILGAIATARYYDNGQKMTVEIQAIIEQALQLDKNENASLLLLASDSFLNNNFQQA
                     LDNWRKVLDSNNDSINRRAIIQSMEMARQMLNSQQ"
     gene            complement(18492..18944)
                     /gene="nrfF"
                     /locus_tag="PWY43_000831"
     CDS             complement(18492..18944)
                     /gene="nrfF"
                     /locus_tag="PWY43_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDL2135730.1"
                     /translation="MRNSIISIGFLQKIRKILPLVFGLLLFVGTVQAEMVDTFQFQNE
                     ADRVRAISLARSLRCLQCQNQNLVESNATAAYNMRIEVYEMVNQGKSNEEIIRIMTER
                     FGSFVNYDPPFTPQTWVLWGLPVGLFSLLFLAILWRTSKNKGNKNENA"
     gene            complement(18934..19467)
                     /locus_tag="PWY43_000832"
     CDS             complement(18934..19467)
                     /locus_tag="PWY43_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL2135731.1"
                     /translation="MKKILLLIPLLLLVVIVGFLTVPLMNKDALAPTEDWQGKPFPEF
                     VGKNLLDSNAHLNNNSLPKEPYILNVWASWCTWCIKEFPILLELKQKGVPIVGLTYSD
                     RPNDALNALKNWGNPFSLIIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
                     DFAQDFLPKLEALRNEK"
     gene            complement(19464..21368)
                     /gene="nrfE"
                     /locus_tag="PWY43_000833"
     CDS             complement(19464..21368)
                     /gene="nrfE"
                     /locus_tag="PWY43_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HDL2135732.1"
                     /translation="MLPELGYFSLLLAAISAIFQVGFALLGEVRKQYHWLALAPLFTY
                     LQAAFTTFSFSTLAYAFLTDDFSVIYVAAHSNSQLPDFFKFAATWGGHEGSMLFWLTA
                     LVIWSAVFCKFSQKIDRLFANRTLAMLGLIALGFLLFILLVSSPFERGFPPPPEGRDL
                     NPMLQDIGLIFHPPLLYLGYVGFAVSFAMIVAALVSGGMDAAIMRWLRPWTMISWGFL
                     TAGIILGAWWAYYELGWGGWWFWDPVENASLMPWLLGTALVHSLIVSQQRGIFYYWTI
                     LLAIFAFALSLLGTFIVRSGVLTSVHAFAVDPDRGMALLILFFGLSFIALALFAFRAN
                     LWQGKVRFNLLSKETAFLLINGLFSVAASVVLLGTFYPMIFTVMNWGSISVGAPYFNS
                     VFTPLALLLMIVMGFAVVLGWKQISNKQILVKLWLLPIAVGLALGLIYASISQREHFA
                     LQPLPTIFISLAIWIILTHLPYSQKMWQIRPLAMRLAHIGFAVCVIGAMMNSYYGDEI
                     GVRLKPNQAAELAGYTFKYEDYHDLIGPNYTAEQAVFSISRDEQLLATVTPERRYYDV
                     RTMTMAEVGLFHHNLDDIYIVMGDKFGNLEYAFRLHYKPYVQALWLGGMMMVMAALLA
                     LFGYRRENKK"
     gene            complement(21638..22585)
                     /gene="nrfD"
                     /locus_tag="PWY43_000834"
     CDS             complement(21638..22585)
                     /gene="nrfD"
                     /locus_tag="PWY43_000834"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDL2135733.1"
                     /translation="MNEYVPFQTPNLVWDGTIAIYLFLLGISSGAVQLAIAYRNSGVK
                     MEKPSQNWIIRSAAILGTLPTIIGLLLLIFHLTKPWTFWKLMFNYNFTSVMSMGVMLF
                     QLYMAVLVVWIAIMFKDWIVRYLPVVSGLIDFAEAKLLKPIEFILFVLAAVLGAYTGF
                     LLSALVSYPMLNNPVLPALFLASGASSGIAATFLVILLAGKLSGESHEVHFMHKFEVP
                     IMVTELGLIVAFFVGLHFGGADKSLALTNALNGFWGNVFWIGVMLIGIAIPLVANIFG
                     SHRLKHNVKFIILISVFDLIGVLCLRYFILYAGQLTVAS"
     gene            complement(22585..23262)
                     /gene="nrfC"
                     /locus_tag="PWY43_000835"
     CDS             complement(22585..23262)
                     /gene="nrfC"
                     /locus_tag="PWY43_000835"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDL2135734.1"
                     /translation="MTCTRRDFVSGAGAIAVVASTGLATSITLATEKEQKKIRYAMVH
                     DETACIGCTACMDACRTTNKVPEGVSRLEIIRSEPFGEFPNVQYEFFRQSCQHCTNAP
                     CVSVCPTGASFIDPETGIVDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
                     TNLAEGKQPACVEACPTKALTFGDMNDPSSDIFHKVKNNPVYRTKTALGTEPNLYHIP
                     FGKGEHR"
     gene            complement(23262..>23411)
                     /locus_tag="PWY43_000836"
     CDS             complement(23262..>23411)
                     /locus_tag="PWY43_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDL2135735.1"
                     /translation="CHEIHPEKDPMKDIPEKDRIKLCTDCHTKIHSGEFKNPEMKATE
                     AKGTK"
BASE COUNT         7179 a         4983 c         4763 g         6486 t
ORIGIN      
        1 ttttattgtt aatttagccg ctccattgtt attagccgtt tttattgggc tggaaggata
       61 aatcacaagc ggtgattttt atcccacttt ttgcaaaaaa tggaataaaa atcaccgctt
      121 gtttgctttt atttatccat cttcgctgaa ttttccgcta taatccttaa gttttccccg
      181 aaacttggga tatttatttt caaaaggata attttatgat gcgttctcac tactgcggtg
      241 cattaaaccg ctcacacctt ggacaaacag ttaccttaag cggttgggtt catcgtgttc
      301 gtaaccttgg tcgttttatt tttatgcaaa ttcgtgatcg tgaaggcatt gtgcaggtgt
      361 ttttcgatga gaaagatgaa gcacttttca agcaagcatc ggccttacgt tctgaggcgt
      421 gcgtgcaaat tcaaggtgaa gtgattgccc gtgatgaatc gcaaatcaat aaagagatgg
      481 caaccggtga aattgaagtg ttagtaaaaa atttagtggt gtataacaac gctgaagtat
      541 tgccgttaga cttcaatcaa aacaataccg aagaacagcg tttaaaatac cgctatttgg
      601 atttacgccg ccctgaaatg gcagaaaagc tcaaaacccg tgctaaaatc accagttttg
      661 tccgccgttt tatggacgat aacggtttct tagacattga aaccccgatg ctgaccaaag
      721 ctactcctga aggtgcgaga gattacctcg tgccaagccg tgtgcataaa ggcaaattct
      781 atgctctgcc acaatcacct cagctattta aacagttgct aatgatgtcg ggcttcgacc
      841 gttattacca aatcgtgaaa tgtttccgtg atgaagattt gcgtgccgac cgccagccgg
      901 aatttaccca aatagacgtg gaaaccagct ttatgactgc cgaagaagtg cgtgcattaa
      961 tggaagcgat gatccacggc ttatggaaaa agaccttagg cgttgatctc ggtaaattcc
     1021 caatgatgac gtggaatgaa gcaatgaccc gtttcggttc agacaaaccg gatttgcgta
     1081 acccactgga aatggttgat gtggcagata ttttaaaatc ggttgatttc aaagtattta
     1141 gcggtccggc gaatgatccg aaagggcgtg tagtggcact tcgtgtgcca aacggtgcag
     1201 ctctaacccg taaaaatatt gatgactaca cccaatttgt cggcatttat ggggcaaaag
     1261 gcttggcttg gttaaaagtg aatgatatga acacaggttt agaaggcatt caaagcccgg
     1321 ttgcaaaatt cttaaatgca gaagtgctaa aagccttagt taaacgctta aacttgcaaa
     1381 aagacgacat tgtattcttc ggtgcggata actggcacgt tgcgactaac gccatcggtg
     1441 cgttacgctt aaaagtgggg aacgatttag gcttaaccga taccacagcg tggaaaccgt
     1501 tatgggtgat tgatttccca atgtttgaac gtgatgatga aggcaactta tcggcaatgc
     1561 accacccgtt cacttcaccg aaagaattat cgccggaaga attagtgcaa gatccggaaa
     1621 atgcggtggc aaacgcttac gatatggtaa ttaacggcta cgaagtgggc ggtggctcgg
     1681 tacgtatttt cagccagcag atgcaacaaa ccgtgttcag cattttaggc attaacgaag
     1741 aagagcaaaa agagaaattc ggtttcttat tagatgcgtt aaaattcggt actccgccac
     1801 acgcaggctt agccttcggt ttagaccgct taacgatgtt gattacagga actgataaca
     1861 ttcgtgatgt catcgctttc ccgaaaacca ccgctgcagc gtgtttaatg acagaagcac
     1921 caagttatgc gaatccgaaa gcgttggaag agttagcaat ccgtacaatt ccacaagagt
     1981 aatcggtagg ggcgaaatat ttttcgccct ttttatttaa caagcggtcg aaaaagtgaa
     2041 aaactttgca aaagtaataa tcgcaaaatt attctctaaa taacataata aagactcttt
     2101 tctcttctct atgttactac taaatatccc ctattatttt aattattacc ttatttgggt
     2161 attgagcaga tttatgccga acaagataat agcgctgaat aattgtgtgg tatttaaatg
     2221 aatatttaat cgatttaaat aatcttttta atagcgctca aatctgatct acttacatat
     2281 ttatacaggt ttcaaataat aatgaagcat actttcaagc tctctcttct agcaacatca
     2341 attgctattt ctctttcacc aacattactg gcatctattg tacgtggtga tgtcgattat
     2401 caatattttc gtgatttagc agaaaataaa ggccaatttt ttgttggggc aaccaatatt
     2461 ccagttattg ataaaacggg caaaaacatc ggtacttttt tacaggttac accaactaag
     2521 caggttgaag tggaatcaaa tatttcacct aagccgaatg taagcctatc tgaatgcttt
     2581 gataatgaaa gtttacccgg ttgttatgaa tttctctatg gtggaggtga tgaagactct
     2641 aatgatctac ctgtttttga aacagtaaca ggtgagacta tgtctattcc aatgattgac
     2701 ttctcttcca ttactcgtca aacagggatt gcaacattag ttaatccaca atacgttatt
     2761 agtgtaaacc acaaccaagg ctatcgggac attcaatttg gtgattcatc tagcgaaaaa
     2821 gccgacgacc atcattacaa ttatagtgtg gtgcgccgta ataactatat gcctgatcca
     2881 aataaagaaa gaacagatct cgggaaaggc atagaaaaac gtgaacgtaa tgacgaagga
     2941 gaaaaactga ttttagatgg ttcaggtaac ccaacgcccc gctgggacta ccacgcacct
     3001 aggttatcga aattagtaac tgaagtcgct ccagctaatg agattgaacg cactaccgaa
     3061 aatgtacaag atttttatag tgtatttagc gaccctttta ttttccccat gtttattcgt
     3121 gccggttctg gtagacaagc agtgattaac aacgaaagag atcgcttaaa caacgggcgt
     3181 attgaagtcg gtaacggtcc ttttttgaca ggaggtagcg ttctgcctgt tactaatggc
     3241 gatccaagcg ttccaggcag cgattttaac agccgtattt tagtagcaaa aactgaaaat
     3301 aatactataa acgatgtatt taaagaccac ggctacggtc ccttaacaac tctcggttta
     3361 cctggcgata gtggctccgc tctttttggt tatgatgttc gtaccaaaaa atgggttgtg
     3421 ttaggtgtat atagtgacta tttctcagag aataatacgc ctgggggaga tgtttataaa
     3481 tcttactgga actatcacca cccacattat gtacgtgctt tagaaaaaga aaataatgca
     3541 ggcgctatta atgcaaatgg tgctagactc acttggacac cttctggcaa taccagctca
     3601 atcgtaggag ggaatgctcc tcttacagtt aatttagcag atacttcatt accaacactt
     3661 caaggtcata atggtaatac aggcgataac ccttggaatc ctcaactcca acacggtaaa
     3721 acattccata ttttgggtga aaacaatacg ctaagtctca cagagaatat caaccaaggt
     3781 gcaggagcaa tccattttca tggtaatacc actgtggaag gtatgaaagc aggcattact
     3841 tggttaggag ccggtgttga tattgataaa gataaaaatg tgatctggaa aatcagtaac
     3901 cctgctggtg acagattatc aaaaatcgga caagggacac taactatcaa cggcaaagga
     3961 gaaaacaaag gctccgttag cgttggtgac ggcactgtta tattaaatca acaagcggat
     4021 gaaaataaca aaaaatctgc attttcagag ctaggtattg tgagtggcag acctaccgta
     4081 atcctcaatt catcagatca aatggatcct aacaatatct attttggtta tcgtggtggg
     4141 cgcttagatg tcaacggcaa tgatctcacg ttccgccgta ttcagaatag cgatgaagga
     4201 gcgatggttg tcaatcataa tacagcccaa acagcaaaca ttacactaac cggcatagtt
     4261 agtccaacaa ttgaccaagt gaaagttcaa gcaattacta gtccagatca ggtttctcgt
     4321 catttggaca aagatatttt cacatatcaa cgttttccac acttgccaac taattttatc
     4381 cgtttgaaaa aacctttaag cgttgcagag atcacagcta tttttaatgc gcgtaaagtt
     4441 ttcatcgatt tagactttgc tactggtctc attcgtgaaa acgaatactt tgaagtactc
     4501 aaggtaacag atgaaagtgc tgcaaagcaa caaatacttg ctgaaaaaat caaagcagca
     4561 gaaggcaccg tccaaggttt taacggttat tttggtgaaa ctgatcctag caaaaccaat
     4621 ggtcgattaa acattacaat gaacagtaat gaagtagcaa acggcaaatt gcttatcact
     4681 gggggaacga acctcaacgg tacgctttct gcagaaggta caagcgaaat cattttatcg
     4741 ggccgtccaa cacctcatgc ctatgataaa gtagcgaata aagaagtcct gattgaaggc
     4801 gactggatca accgatcgtt caacgcaaca atctttgcag ctaaagacaa tggcaagctt
     4861 gaaatcagcc gtaatgttgc caatattaac ggtaatttta atttaacaga taatgctacc
     4921 gctcaaattg gttttaccca aggtacctca caagcatgta ttcgctctga ccgcacaggc
     4981 gtagcaacct gtaatattaa cgctacttta tcagagactg atttaaacag ctgggagaga
     5041 accaaagtgg ctggcaatgt ctctttagct gacaattcaa cattttcgtt aggcagtaaa
     5101 gcagacttaa ctggctcaat tacagctcag gagtccacta aggttcaact taatgatgga
     5161 agtattgcta atttaaccgg cttgagtaca acaggaatat ttaatggcgc caatggttca
     5221 accgttaatc tttctggggt atggacaacc ttaaccaaca ctgttgtaga tgtcttgaat
     5281 gtcgcaaatg gtagccaaat ccatttaaac actccaactg caacaaaaca gaatacgctt
     5341 accgctaata ccctcacagg gaacggcata tttaaatttg tcgctgatct gacgaaaaca
     5401 gcgagcgata tagtcacaat taaagacacc gcaagcggtc aattttcgat agaaattagc
     5461 acaaatggta gcgcccattc agcccaaaag attaaattat ttgaaacaac aaatgaagaa
     5521 gacttttctc ttaatttagc aaatgaagtg cgctcaggcg aatatcgcta ccatttactc
     5581 aaagaaggga atgtgtatta cctcaatcca gagaaagagg aagaacctaa aattactcaa
     5641 aaagggccaa gtgaaaccgc tgacccatta cctgaataca ccggggatat tgagtattct
     5701 gaaaaagggc cgggtgaaac tgctgatccg ttacctgaat acacaggaga cattgagcat
     5761 tcagaaaaag gatcgggtga aaccgctgag ccattaccta aatacaatgg aaatgcacat
     5821 ttgatcgcat ccgagttaac tgcgcaaact aatgctgtat taggtgtaac tacagtatta
     5881 gatcatgtaa ttcattcgga agggacccaa aattggaaag tatggtctaa aacagattat
     5941 caaacgacag agcatcgctc tgccggatat ggatttaaac aagatgcgaa cgttatccat
     6001 ctaggattag agaaagcctt agataatcag gtcacacttg gtatgatatt atctcaatca
     6061 aaagcgagaa ataattttga ccattattat agcggtaaag gccgcttaac aatgctatca
     6121 atgtatgcga agaaaacatg gcaaaatggt gtttttgttg cgatagatac cggttttggg
     6181 aaagcaagca atcgcttaac ttatcaagca aatacagtaa aattagatcg ttcagtattt
     6241 gtaacagggc ttagtatcgg taaagcatgg gaatctgcaa acgtgaatat aatcccatca
     6301 tttagcgccc gttatcatca tttatcttca gcaggtaatc aacttgtaga tgcaaaaatc
     6361 gaaacaaacg ctgttgatct tctcgctctt caagctggtc tgtcaattaa taaaacgctt
     6421 gaactaaatg gcctgcaaat aaaaccggaa ataggtagct attttgttga tgcaagccat
     6481 gggaaattaa gaacacgttt caataattta caaatagagc agcaaatggg gcgttatttt
     6541 aaacaagaag caggtatatc cgctaattat cgcaatataa acgcaggaat tcaggcaggt
     6601 ttcttaacgg ggaatacgct aaataaacag cgttatatct cgttcaaagt tacatatgaa
     6661 tggtaaatat tgagattaag ggctatccat tactaaatgg catagccctt tatttactcc
     6721 tcaacaatgt aaattaccca tcaaaacaaa ctctgctgcc caagcaaatt ctccggcaca
     6781 ggtttgattt gaaatccttt ttccgccaaa aagcctttta gggtttgaat attataaaag
     6841 gcgtggcttt tagtggtgtt ttcaaagtag agatagagcc tgtcaaattc cgttctgcgg
     6901 ttaaaaagca attctgccaa gccttttaat tcctcgtctg aatagcggta atcgtggcat
     6961 tctgccgcac tctgcccttt ccaccaattc gggttacgac catgtaagcg taaataagca
     7021 gtacgttggt tggcaaaaaa gtgaaatgcc ggtaagccga tattttgtgg gtaatccaca
     7081 ttacaccaaa tcagattaga gcttttcgca aaggtatcaa acaccgccgg aatatgccag
     7141 cttgggtggc gaaattcaat cgctaacgga taatccgcaa accattggca caactcggca
     7201 aggtaacggc ggttaagtgg cgtacgttca aattgagttg ggaactgaac aaataaattt
     7261 gccaataacc cttcagcggt gaacggagct aacgcattaa gaaataattg tgcttgatct
     7321 aaagtcgccg tgcgttgatg gctgaaatct tgatgcagtt tcaccgaaaa attcagccga
     7381 ccttccgctt tctgcaccat tccttcaaag gctttctgcc cgatgggggc gtggaatgtg
     7441 ctgttaattt ccagcgtgtc gtaatgttgg ctatagacaa agagaaaatc ctcttttgca
     7501 gtgccgaatg ggtaaagcgt gccgattaaa tctgtgtcgg catagccgcc ggtgccaata
     7561 taaatatgag ggagcattgt gtttattctt tcaatttttt gcaaatttta atggtttttt
     7621 aaccgcttgc aaactgagaa taaaagagta agatttggct atcttttctt atttcatcat
     7681 cattatgtgg atttggttta ctctgctggc agccgtggct caagccggtc gcaatgcctt
     7741 tcaaaagcaa ttaagcgtca atgtgcctgt attgggggta acgctcgccc gctttttcta
     7801 tgctgtgccg ttggcaagcg gttatctcta ttttttatac caatatgacg ataacctcaa
     7861 cattccccat tttccaccgc tgttttttgt atatgctatc ggggctgggt tggctcaaat
     7921 tctggcaact tcgctaatgg tgcaactttt tcacttaaaa aattatgcaa taggtgtcgg
     7981 gctggcgaaa agcgaggcag tatgggcggc tttactgggc gtgttatttt ttggtgtaac
     8041 catcagtggc attggctggc ttggcgtggt actaggtgga gtggcgattt tcttaatgac
     8101 cggcagcggt aatctacgcc aactttcgtg gaaaacactc tttctcggca tagccagcgg
     8161 tttatgtttc gccctcacat cacttttggt gcgagaggca agcctgcaac tcaacagcca
     8221 ctatttgctt tcagcggcgt gggtactttt tagcgtgatt agctttgagg cagtgatatt
     8281 ggttatatac ctagctcttc tccaaaaaga gacactgatt aaattctttc gttttccaaa
     8341 attaggtatg ttgaccagcc tgttttcatt tctcggctca ttcggttggt tcaatgcaat
     8401 gagcctgaac cacgttgcct ttgtaaaaac gcttggacaa gtggaaattt tctttattct
     8461 cggtatttcc tattttgtgt ttaaagaacg gcttaaaatt caagatttta tagggctgat
     8521 tttagtagtg attgccgcaa tcttggtggt gctgggctag atcggatttt tacgtttttt
     8581 atctagcttt gtagggtggg cataagccca cgcaaaacca ataaatcatt tatccgtggg
     8641 cttacgccca ccctacgata cctaaaaaaa ttaaatttca catcttagta ataaaatgaa
     8701 ctataaaaat cctcatagta tccttgtggt gatttatgct aaaaataccc aacgagtatt
     8761 gatgctacaa cgcaaggacg atcctgattt ttggcaatcg gtgaccggtt cgatcaatat
     8821 tggagaaaca ccgctggaag cagcagaacg ggaagtgagc gaagaaaccg gtttgcaaac
     8881 ttttgagcaa aaacaaccgc ttgttgattg ccaaaagcag atagaatttg aaattttccc
     8941 gcattttcgg tataaatacg cacctaatat cacacattgt gtcgaacatt ggtttttgct
     9001 gccacttgat agtgagcaag agcctatttt aaccgaacat ttggcatatc gttgggtttc
     9061 agtggaagat gcggttcgac tgactaaatc tcccaacaat gcccaagcaa tacaagaata
     9121 tttaacatca aaataaggaa acaacagaat ggcaggccat agtaagtggg caaatattaa
     9181 acaccgtaaa gcggcacaag atgcacaacg cggtaaaatt tttactaaat taattcgtga
     9241 attggtgact gccgccaaac tcggcggtgg tgatacctct tccaaccctc gtttgcgtgc
     9301 ggcggtggat aaagcgttat ccagcaatat gacccgtgat accatcaacc gtgcaatcga
     9361 gcgtggtgta ggcggtggcg atgataccaa tatggaaacc aaaatctacg aaggttacgg
     9421 cccgggtggt acagcagtga tggtggaatg tttaagtgat aacgctaacc gtacgatttc
     9481 ccaagttcgt ccaagtttca caaaatgtgg cggtaaccta ggtactgagg gttcggtagg
     9541 ttatttattt agcaaaaaag gcttaatttt agtggcaaat gccgatgaag atgccttaac
     9601 tgaagctgct attgaggccg gtgcggacga tattcaacca caagaagatg gtagctttga
     9661 aatttatacc gcttgggaag atttaggctc agtgaaagac ggcattgagg ctgcaggctt
     9721 taaaattgag aatgcagaag taacaatgat tccatcaact accgttgaac ttgacgcaga
     9781 aactgcacca aaacttcttg atttaatcaa ccgccttgaa gattgtgatg atgtacaaaa
     9841 cgtttatcac aatggtgaaa tcagtgacga agtcgctgca ttactgtaat aaaaaaatca
     9901 cgcaaaataa gcataattta ggttatgctt attttttatg aacttttttc ctatattact
     9961 gactaaccta atgtagttta tatgctacta ctttactata acattttaag gagagtctat
    10021 gaaattagtg aaatttgtcc ctgccctagc agcaacttta gtattagccg cttgctctaa
    10081 tgcaaacaca caatctgctc ctcagcaagc tcaggacgct gtgactaaag cggctgaaca
    10141 agcaacacag caagtgaatg aaattgcagg taatgcacct cgtgcaagtg gtgcagtaaa
    10201 agtagaaaaa gcaactgatg gtgcaaaatc aattgtatat cgttgccaaa gtggtaaacg
    10261 tgtaactgcg aattatgtct tccaaggaga agaggcaaaa gcagtaaatc tattagtcca
    10321 aagcggcggt aaaacacaaa agatcccaac attaactcgt gatgcctcaa accaagattc
    10381 tacttcattc aaaacggata aatattcttg ggatttagac acaggcttta ccttatcatc
    10441 taatggccag tcaggtatgc taaccaaata tggtgctaaa acagatacca tcgttgctaa
    10501 attatgtgat gtagataaag cattaacctc aaaattaaat aaataatctt tcttgatttt
    10561 actactcata agcgagaaga taatatcttc tcgcttattt tattgcacaa agatggctat
    10621 tattttagga attgaccccg gctcaagggt aacaggttac ggtgtgattc gccaaaacgg
    10681 gcgacagctg gaatatttag gaagtggggc tattcgaacc acggctgaag atctgcctac
    10741 ccgcttaaaa cggatttatg caggcgtgag tgaaatcatc actcaatttc aaccaaatat
    10801 gttcgccata gagcaggtat ttatggcaaa aaaccctgat tctgccttaa agctcgggca
    10861 ggctcgagga acagcgattg tagcagcggt taatcacgat ttacctgtgt ttgaatatgc
    10921 cgcccgatta gtcaaacaaa ccgttacggg aattggttct gctgataaag tacaagtgca
    10981 agatatggta acccgtatgc ttaaactctc cgccaaacca caagccgatg cggctgatgc
    11041 attagccatt gcgattaccc acgctcattc aatccaacat tcactgattg ttgctaagca
    11101 aagccagcag aatgtgagta gtgagaaaga gcaaatttta gcactaatga aaacccgtta
    11161 tagccgggga aggtttaggt taaaaggcta actcaatata aaatgaccgc ttggaactaa
    11221 tgtttcaagc ggtcattttt tgagaaaatt ttacaattac tcgccttcaa aaggagctga
    11281 gaaacgttca attctcgccc caatctgacg taatttttct tcgatgtgtt catagccacg
    11341 gtctatgtgg taaattctat ccacaatcgt ttcacccgtt gcaatacagc ctgccaacac
    11401 taagctgatg gaagcacgca agtcagttgc cattacttct gctgatttta actgctctac
    11461 accatagcaa attgcagtat taccttcaat ttccgctttg gcacccatac gaatcagctc
    11521 aggaatatgc ataaaacggt tttcaaaaat cgtttctgta atacggcttg tgccttctgc
    11581 aacggtattt aataaggtaa attgagcttg catatcagtt gggaaacctg ggtgtggcat
    11641 agtgcgaatg ttcactgctt tcgggcgacg acctagagaa tcaagcgtga tagtattttc
    11701 cgtcacatca acctgcattc cagcttcacg taatttttcg atcactgcat caagagtgtc
    11761 tgctttggtg ttacggcaag taattcgacc accggaaata gccgctgcca ctaaaaatgt
    11821 accggtttca atacgatccg gcactacgct gtgttcgcaa ccgcctaaat gctcaacccc
    11881 ttcaatggta atcatatcgg tacctgcacc agaaatttta gctcccatcg catttaagaa
    11941 atcggcagta tctacaattt caggctcacg agcagcattt tcaataacag tagtaccttt
    12001 tgctagagta cctgccatca tcaccgataa agttgctcct acgctcactt tatccatata
    12061 gattcttgca ccgtttaaac gaccgtttga agttgctttg acatagcctt catcaagctc
    12121 gattttcgcc cccatttttt caagaccgga aatgtgcata tctaccggtc ttgccccgat
    12181 agtacaaccg cccggtaaag aaacttgccc aagttggaac cgggcaacta atggagctaa
    12241 cgcccaaatt gaagcacgca ttgttttcac taattcgtaa ggtgcaacgt aattattgat
    12301 cttactcgca tcaatatgta ccacgccttt atcgctacct ttttcaaccg ttacccctaa
    12361 ttggcgtaag attttaaaag tggtgtccac atcttttaaa tcaggcacat tttttaacgt
    12421 aactgttttt tcagccaaaa tagcggcaaa taaaataggg agtgcggcat ttttcgcacc
    12481 tgaaatatcg acagtaccat ttaaagaaaa tggaccatac acacgaaatt tttccatttg
    12541 ggagatccta aaaaataata atttgtaggg gcgaaaaatt tttcgcccaa ttttccaacg
    12601 tttacaaaag agtttgggtg aatagtgatt cgcccctaca tttcatctat ccaaccatat
    12661 ttagcaaacg ctcagttttc catttttcca cgctgaatac tttaatggtt aaagcgtgaa
    12721 tcgcattagt agtgaaatac tcagctaaag gggcataaac agcttgctgt tgttttactt
    12781 ttgaaagaga agcaatgctg tcacttacta caataacgcc aaagtgggca ttctcgcctt
    12841 gagcgtggac ttctgttgca tcagataatg cttgttttaa aatttcttca atttgtgatg
    12901 gattcattct gacttcctaa aatatgattg gtaactcact caaataataa attgctcaaa
    12961 ccaaccgtct aaatcgtata atgcagccaa agttctgaca gaatctggcg tattaatcaa
    13021 actgtttggt atgattttat ggtaatgatt cagtaattca gcaagtaagg taaaaccggc
    13081 agaatcaatc cgagtgattt cttttaaatc ccaatatagg ctttgtttcc ctgttggaga
    13141 taaaaaagaa gcacgttgct tccataaagg gagcaacgtg ttacgagtta attcgcctga
    13201 tagtttcaca aaaagacttt cgttattttg ctgaatgtcc cattgtaatg ttttttgagg
    13261 cataactaaa ctacttattt taaagtgata ggctgttttg cagattgtgc cacttgtgca
    13321 attaaggcat caatcccttt ttgacgaatc acacccgccc attcgttttg cttggttgca
    13381 accatactta ctccttcagc ggccatatcg tatgcttgcc attcacctgt tttgctgttt
    13441 ttacgccatt taaaatctaa tttgatcggc tgtgcagcac ctttacctga cacgtttaca
    13501 cgaatgctta cgatagattt accatccacc gatttcgggc tttcaatttg cacactttga
    13561 tcttgatact gggttaacac ctgagcataa gactgctcaa caaattgacc aaaagcatta
    13621 aagaatgcct ctttttgtgc atcagtcgca gccgataagt ttttccctaa cactaattgg
    13681 cctgcatatt tcacgtgaac gtgtggcatt aaatcgttac gcacaatggt acgcaaatat
    13741 tcagggtttg atttaatttt cgcttgattt gctttaatat cgccaaataa tttatctgcg
    13801 gtttgttgca ttaatacata agggctagtt tcagcaaatg cggtggttga aaataatgca
    13861 gaaactgcca ctaagcctgt tacgataatt tttttaatgt tttttaacat tattgatgct
    13921 ctccaaaact ctaaattaga ggttgttgta cttaattact ctgcttttgg ttcagcagaa
    13981 tcacttgctt tttcatcttc agttttatct gactttttat caccatataa gaactgaccg
    14041 attaaatctt ctaacaccat tgcagaattg gtatctacaa aagtatcacc ttctttcatc
    14101 attgcagttt caccttcgat tacgaagcca acatttaacg caacgtattg ctcgcctaat
    14161 aagcctgagg ttttaattga gagcgagctg gtatcgggaa tttgattaaa actttcattt
    14221 accgctaatg ccactttcgg ggtgtaggtt ttgttatcaa gtgaaatatc agccactcgg
    14281 ccaactacca caccgccaac tttaataggc gcacgaacct ttagtccacc aatattatca
    14341 aaagttgcat acagggtgta agttttctca tttgagaagc cctgcacatt tgctactctt
    14401 aagcctaaaa agacaagagc tgcaagccca agtagtacaa ataagcctac ccaaaattca
    14461 tatttaattg attgacgcat tgtctgttcc tttcaaaaaa tcaaccgccg aacattatcg
    14521 cagttaaaat aaaatctaat cctaaaatca ccagtgaggc atttaccacc gttcttgtgg
    14581 tcgcttggct gattccttct gaagtcggta cgcaatcgta gccgttaaat aaggcaatcc
    14641 agaccaccgc aaaggcaaaa atcacacttt taataaagcc gttaattaaa tcgctggtat
    14701 ttaccgagtt ttgcattacc gaccaaaaac tgccaccatc aacgcctttc caatctaccc
    14761 cgactaatga gccgccccaa attccgatag cggtaaaaat agcagctaac accggcattg
    14821 aaatcacgcc tgcccaaaag cgaggagcga tgatacgtct aagcggatca accgccatca
    14881 tttcaagact ggaaagttgc tctgtggctt tcatcagccc gatttctgcg gtcaatgccg
    14941 aaccggctcg ccctgcaaat aataatgcag taactacagg accaagttct cgtaaaagcg
    15001 agagtgaaac caaagttccc aagctacttt cggcggcaaa atcgaccaat accacatagc
    15061 cttgtaagcc taacaccatt ccgataaata agcccgacag cataataatt aagagcgatt
    15121 gcactcctaa cacataaagc tgtttaatca gtaacggggt atgttttcta aattcaggtt
    15181 taccgattaa tgctccccat aacataaaag cggaacgccc taatgtacgg ataaaattaa
    15241 tcaccaccgc cccgatagag ctaataaaat taaccattaa atagctcctc cgtataatct
    15301 tttgctggat aatggaaccg caccggacca tcagctttac cggctaagaa ttgtaccact
    15361 tgcggatctt cgcttgcctg taaaatttcc ggtgtacctt cagcaatcac tcgtttatct
    15421 gccacaatat aagcataatc ggcaatactt aatacttctt tcacatcgtg agacaccaca
    15481 atggaggtca aattcaatgc ctgatttaac tctttaatca gctcgacaat cacacccata
    15541 ctaatcggat cttgcccggt aaacggctca tcatacataa tcagatccgg atctaacgca
    15601 atcgctcgag caagggcggc acgtcttgcc ataccgccgg aaagttcgga cggcattaaa
    15661 tttgccgcac ctcgcaaccc aaccgcctcg agcttcaata acaccaattt gcgaattagc
    15721 tcttcgggta aacgggtacg ctcccgaatc gggaaagcca cattatcgaa ggtagaaata
    15781 tcggtaaata acgcccccga ttgaaacaac atccccattc gttggcgaat ttcatacaac
    15841 tctttgttat tggctttgca aatatctctg ccatcaaata aaatttcgcc cgattcaggt
    15901 aaaatctgcc caccgattag cctcaacagg gtggttttac caatcccaga agaccccata
    15961 atggcagtca ctttaccttt ctgcacttgc agattcaggt tatcgtaaat cactcgctcc
    16021 cctcgcttga aggtcaagtt ttttacttca accaaatttt ccgtttttgt catcaaatag
    16081 gactcaaatt aataaaagtg atatttatgg tctatcaaat ttgccagaat gtcaaaaaac
    16141 ttttaataat ctgacaaaaa tctgccttta caagcggtca gatttgctaa attttttgca
    16201 aatcggctga actgaaaaag aaaggtcttg tcttagccaa tacgagcgtt ttcactcgta
    16261 gattataaaa acataggata tgaggaacat tcacaatatg aacaaaacaa tgaaaaaatc
    16321 atttttaact gcattcattt taggctcaac cattgccgca atgccggcgt atgcagaagc
    16381 aacattaccc acagccgtaa atgggcaagc tgtgccaagc cttgcaccaa tgctggaaaa
    16441 agttcgcccg gcggtggtga gtattgcagt agaaggtaaa accaaaggcg atagccgccg
    16501 cagtcgtgaa attccggaag aatttgaatt tttcttcggt cctcacgccg atttattcgg
    16561 caaccgctca tcagctccac gcaatttccg tggcgttgga tcaggtgcag tcatcaatgc
    16621 cgaaaaaggt tatgtgatta ccaataacca cgtgatcgac aatgccgata aaattaccgt
    16681 aaaacttgaa gacggacgtg aatttaaagc taaattagtt ggtgctgatc ccctttcaga
    16741 tgtggcgtta attcaagttg aagatccaaa aaacttaact gaaattaaat tcgccgattc
    16801 cgataaatta caagtgggcg attttacagt tgcaatcggt aacccgtttg gcttaggaca
    16861 aactgttact tcaggcatca tctcggcact tggtcgttca accggtcaat ccgatgaagg
    16921 ctatgaaaac tatattcaaa ccgatgcggc agttaaccaa ggtaactctg gcggcccgtt
    16981 aattaactta aacggcgaat taatcggaat taacaccgct attatttctc caagtggtgg
    17041 caatgccggt atcgcctttg cgattccaag taatatggca aacagcttgg tgactcaaat
    17101 tattgagttt ggtgaagtaa aacgtggaat gttaggcatt aaaggcggtg aattgaatgc
    17161 tgaccttgct aaagagttca atatttccac ccaacaaggg gcatttgtaa gcgaagtctt
    17221 accggattct gctgctgcta aagccggctt taaagcaggt gatgtgatta ctgaaatcaa
    17281 cggtcaaaaa atccgtagtt tcagcgaatt acgagcaaaa gtagctactt caggtgtagg
    17341 taaagagatt gaacttactt acctgcgtga cggcaaagaa gccaaagcaa aagtgacctt
    17401 acaatcggat tctgaagcca aagttactgc ttcaaacctc attccggcat taaaaggggc
    17461 agattttaac aactacaatg ccaaaggcat taaaggggtt gaaatttcta acgtagaaaa
    17521 aggctcgatt gctgaaatgc gaagcctcaa aaaaggcgat attatcatcg gcgtgaaccg
    17581 ccactcggta gaaaacattg ccgatttacg caaaatatta gaaaccaagc catctgtggt
    17641 tgcactcaat attgtgcgtg acggtgcaaa tttctatgtg attttgcaat aaaccttttg
    17701 atccactcaa acaaaagccg atgacaaaaa tcatcggctt ttgttattgc tgggaattta
    17761 acatctgtcg tgccatttcc atactttgga taatcgctct gcggttgata gaatcattat
    17821 tgctatctaa cactttacgc caattatcga gagcttgttg aaaattgtta tttaaaaagc
    17881 tgtcggaggc tagcaggagt aaactggcat tttcattttt atccaactgt agggcttgct
    17941 cgataatggc ttgaatttca acggtcattt tctgcccgtt atcgtaataa cgagcggtgg
    18001 caatcgcccc caaaatagcc gctcgttttc cgagtagctt ctcggcatta tcgtagcaaa
    18061 ccagtgccga atcaaaatca ttgcttaacg cataagcctg ccctagttcg taccataaat
    18121 caccgttatt cgggttttct cgaagctggt tttgcagact caaaatatag cgttcatttt
    18181 tcgatgtgcc gtgtgcatcg ctcatctgtt gttgaaaatc ttgatgttgt tgcaaacctg
    18241 attgcaccac ttgataacgt ccggaaagcc catagtaagt cattgctaac aacaaaatcg
    18301 ccacgcaact caggcttgcg attttagcaa gcggtcgatt ttgagcatta ttttgcaaaa
    18361 cgctctgttc aaattgcgtt cgttcaaatt ggtaacgttg ctctgcctca cgctcaaact
    18421 cttgtctgac gtcatctgca aaatgttggg caaatttaac cgcttgttgg tagttttctt
    18481 ggcttaattg ctcacgcgtt ttcatttttg ttgcctttat ttttgctagt tcgccacaaa
    18541 atagctagaa ataacaggct aaacaagcca accggcaagc cccataatac ccatgtctgt
    18601 ggcgtgaatg gtggatcgta attcacaaaa ctgccgaaac gctcggtcat aatgcggata
    18661 atttcctcat tacttttccc ttgattcacc atttcataca cttcaattcg catattgtaa
    18721 gccgccgtcg cattggattc gaccaaattt tggttctgac attgcaaaca tcgcaacgaa
    18781 cgagccagtg aaatcgcccg tacccgatcc gcctcatttt gaaattgaaa ggtgtccacc
    18841 atttcagctt gaaccgtgcc gacaaatagc aacaagccaa atacaagcgg taatattttg
    18901 cggatttttt gcaaaaagcc gatggaaatt atgctatttc tcatttctga gagcctctaa
    18961 tttgggtagg aaatcctgtg caaaatcagg gttgtaaccc ttttgttggt aacgcactac
    19021 accgtgttta tcaatcaaat aactggaagg tgcagaagag actttcaggg tttggatcaa
    19081 aaagcctttt tcgtaatcat caataattaa actaaacgga tttccccagt ttttcaatgc
    19141 gttgagggca tcatttgggc ggtcgctata agttaagccg acaatcggca cgcctttttg
    19201 ttttagctcc aacaaaatcg ggaactcttt aatgcaccaa gtacaccagc tcgcccacac
    19261 attgagaata taaggctctt tcggcaagct gttgttattt aaatgcgcgt tactatcaag
    19321 taagttcttg cctacaaact ctggaaacgg cttgccctgc caatcttcag tgggagcaag
    19381 ggcatcttta ttcattaacg gcacagtcaa aaagccaaca ataactacaa gtaacaggag
    19441 cggaataagt aacaggattt ttttcatttt ttgttttctc tacgataacc gaataatgcc
    19501 aacagggcgg ccattaccat catcatacca cctaaccata aggcttgcac ataaggctta
    19561 tagtgcaaac ggaaggcata ttctaaattg ccgaacttat cccccatcac aatataaata
    19621 tcgtctaaat tgtggtggaa aagccctact tctgccattg tcatcgttct tacatcataa
    19681 tagcgtcttt ccggtgtgac ggttgccagc aactgttcat ctcggctgat agaaaaaacg
    19741 gcttgctcgg cggtgtagtt cggaccgatg agatcgtgat aatcttcata tttaaaggta
    19801 taaccggcaa gttccgctgc ttgattcggc tttaaccgca cgccgatttc atcgccgtaa
    19861 taactgttca tcattgcccc aatcacgcaa accgcaaagc cgatatgggc taaccgcatc
    19921 gcaagcggtc gaatttgcca cattttttgc gaatagggca aatgagtgag aataatccag
    19981 atcgcaaggc tgataaatat ggtgggtaac ggctgcaaag caaaatgctc tctttgcgag
    20041 atggaggcat aaatcaaccc taacgctaaa ccgactgcaa tcggcagcaa ccaaagtttt
    20101 accaatatct gcttatttga aatctgtttc cagcccagca ccaccgcaaa gcccattact
    20161 atcattaaca gtaacgctag tggcgtgaat acgctattga aatagggggc ccccactgaa
    20221 atcgatcccc aattcatcac tgtgaagatc atcgggtaga aagtccccag caataccaca
    20281 gaagcagcca cactaaataa gccgttaatc aataaaaatg ccgtttcttt agaaagtaag
    20341 ttgaaacgca ctttgccttg ccagagattc gctcggaatg caaacagagc aagggcaatg
    20401 aaacttaagc cgaagaataa gatcagcagt gccattcctc tgtcgggatc gaccgcaaag
    20461 gcatgaaccg aggtcagtac acccgaacga acaataaacg tgcctaatag gcttaaagca
    20521 aaggcgaaaa tggcaagtag aatcgtccaa taatagaaaa tgccacgctg ttggctcaca
    20581 attaaactat gcaccaatgc ggtgccaagt agccaaggca ttagggaggc attttccacc
    20641 ggatcccaaa accaccagcc accccagccg agttcgtaat atgcccacca cgcccctaaa
    20701 ataatgccgg cggtgagaaa tccccacgaa atcatcgtcc acggacgaag ccaacgcata
    20761 atcgcagcgt ccattccgcc tgaaacgagg gcggcgacaa tcattgcaaa gctcaccgca
    20821 aagccgacat agcccaagta gagcaaaggc gggtgaaaga tgagcccaat atcttgcaac
    20881 attgggttga gatctcgccc ttcaggtggt ggtggaaagc cacgttcaaa tgggctggaa
    20941 accaatagaa tgaaaagtag aaaacctagt gcaattagcc ctaacatcgc taaggttcgg
    21001 ttggcaaaca agcggtcaat tttttgcgaa aatttgcaaa ataccgcaga ccaaatcaca
    21061 agagcggtga gccaaaacag cattgaacct tcgtgtccgc cccaagtggc ggcaaattta
    21121 aaaaaatcgg ggagctggct gttggaatga gcggcaacgt agataaccga aaaatcatcg
    21181 gttagaaaag cataagctaa ggtagaaaag gaaaatgtgg taaaagcagc ttgcaaataa
    21241 gtgaaaagcg gagccaatgc cagccaatga tattgcttgc gaacttcgcc tagaagtgca
    21301 aaaccaactt gaaaaatggc actaattgca gccaataaaa gtgaaaaata gccgagttcc
    21361 ggtagcataa aaaatccaaa aaatgaccgc ttgtgaatgt aaaaaaagcc ctctccactt
    21421 gtgggagagg gacagatttt tcttcgttca gaagaaaaat cagggagagg ggaattaaat
    21481 aattttgcta aaaataacaa tactccctct ctctgaaaaa tatttctgaa cgaaatattt
    21541 ttcggtctca atactcgcta cgctcgctcc ttcggagccg ttggcaaagc caacgttcaa
    21601 atgtaaacat ttgtcccaca aggggagaga gtatatccta acttgcaacc gttagttgcc
    21661 ctgcataaag gataaagtaa cgcaagcaga gtacaccgat taagtcgaaa acggaaatta
    21721 agataataaa tttcacgtta tgtttcaagc ggtggctacc aaaaatattg gcaacaagcg
    21781 gaatcgcaat accgattaac attacgccta tccagaacac attcccccag aagccgttaa
    21841 gtgcattggt taaagcgagc gatttatctg ctccaccgaa gtgtaagccc acaaagaacg
    21901 ctacaattaa gcctaattcg gttaccataa tcggcacttc aaatttgtgc ataaagtgga
    21961 cttcgtggct ttcaccggat aacttgcctg cgagtaaaat cactaggaaa gttgctgcaa
    22021 tacctgacga tgcccctgat gccaagaaca atgccggtaa caccgggttg ttgagcattg
    22081 gataactcac taacgccgag agtaagaaac cggtgtaagc accaagtaca gcagccaata
    22141 cgaataagat aaattcaatt ggttttaaca gcttcgcttc ggcaaaatcg atcaaaccag
    22201 agaccacagg tagataccga acaatccaat ctttaaacat tatggcgatc caaaccacca
    22261 atactgccat ataaagttgg aataacatta cccccatcga cattactgag gtaaagttat
    22321 agttgaacat taatttccag aatgtccaag gtttggttaa gtggaaaatc agtaacagta
    22381 agccgataat ggttggtaat gtgccaagaa tagccgcact acgaataatc cagttttggc
    22441 ttggtttttc cattttgaca ccgctattac ggtaagcaat cgctaactga accgcacctg
    22501 atgaaatacc aagtaggaat aagtaaatag cgatagtgcc atcccaaaca aggtttgggg
    22561 tttggaaagg tacatattca ttcattatct atgttctcct ttaccaaatg gaatgtgata
    22621 taaattcggc tccgtgccta atgccgtttt ggtgcgataa accggattat ttttcacttt
    22681 atggaaaata tcgctacttg gatcattcat atcaccaaag gttaaagctt tagtcggaca
    22741 agcctctaca caagcaggct gtttgccttc tgcaagattg gtatctcggc aaaagttaca
    22801 tttatccgcc gattttttct cagggtgaat aaaacgtaca cgatacgggc aaaccgcaat
    22861 acagtattgg caaccgacac acaaatctga gtgtacatca acaatcccgg tttcaggatc
    22921 gataaatgaa gcaccggtcg ggcaaacact cacacaaggt gcattggtac agtgttggca
    22981 agattggcgg aaaaactcat attgtacgtt cggaaactca ccgaaaggct cactacggat
    23041 aatttctaaa cgagaaaccc cttccggcac tttgttggtg gtgcgacaag cgtccataca
    23101 agccgtacag ccgatacagg cagtttcatc gtgaaccatc gcatagcgga ttttcttttg
    23161 ttctttctca gtagcaagag ttatggaggt ggcaagccct gtacttgcca caactgcgat
    23221 agcacctgcc cctgagacga aatctctacg ggtgcaagtc attattttgt tccttttgct
    23281 tctgtggctt tcatttcagg atttttaaat tcgcctgaat gaattttggt atgacaatcc
    23341 gtacaaagtt taatgcgatc tttttcagga atgtctttca tcggatcttt ttccgggtga
    23401 atttcatgac a
//
LOCUS       DANKBV010000028        23151 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.028, whole genome shotgun sequence.
ACCESSION   DANKBV010000028 DANKBV010000000
VERSION     DANKBV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23151
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.028"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..200)
                     /locus_tag="PWY43_000837"
     CDS             complement(<1..200)
                     /locus_tag="PWY43_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDL2135736.1"
                     /translation="MTATKKTLSDTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIY
                     SYISPKTGKRSTKNRIGSYPQM"
     gene            complement(403..478)
                     /locus_tag="PWY43_000838"
     tRNA            complement(403..478)
                     /locus_tag="PWY43_000838"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(443..445),aa:Ile,seq:cat)
     gene            complement(572..1309)
                     /gene="pgeF"
                     /locus_tag="PWY43_000839"
     CDS             complement(572..1309)
                     /gene="pgeF"
                     /locus_tag="PWY43_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDL2135737.1"
                     /translation="MDKIIPNWNVPKHIHALTTLRTGGVSKPPFDSFNLGDHVNDEPQ
                     DIAQNRALLVEKFQLPQAPLFLTQTHSTRVIELPYSGDNIKADAIYTNQPNQVCLVMT
                     ADCLPVLFASQDGSEIAAAHAGWRGLCDGILEATVEKFNCPPHEISAWLGPAIGPNAF
                     QVGSEVADQFCAFDPRAKEAFIEDSTTSGKFLGNLYQIATQRLNKLGITAISGGEYCT
                     YSQPELFFSYRRDKQTGRMATLIWRTE"
     gene            complement(1341..1727)
                     /locus_tag="PWY43_000840"
     CDS             complement(1341..1727)
                     /locus_tag="PWY43_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL2135738.1"
                     /translation="MTVIHTENAPAAIGPYVQAVDLGNMVLTSGQIPVNPETGEIPSD
                     IVQQTRQSLNNVKAIIEQAGLTVADIVKTTVFVKDLNDFAKVNAEYQAFFQENEHPNF
                     PARSCVEVARLPKDVGIEIEAIAVRR"
     gene            1808..2809
                     /gene="yejK"
                     /locus_tag="PWY43_000841"
     CDS             1808..2809
                     /gene="yejK"
                     /locus_tag="PWY43_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDL2135739.1"
                     /translation="MSINVSEIVLHQLQKTEGENPELNTFLRENLLAISPEVDQMMLQ
                     LHQAYQSKAKAYGIFKPESVFAQQLNRLLEQETDFLPFSHSCAKMLAAELIKYPFADG
                     GTFILCRYTFLATEYLFIALIDSRNSMLVDEQLEIKRTQYLEIAQYDIACRINLTELK
                     LDATSNRYLTFVKGRVGRKIADFFMDFLSAEEGFNPQLQNQTLLQAVSDYCEQGELSA
                     PQTREVKKQVFEYCKGQINSGEEIALNELSNALPTLNEIDFAQFTQEQEYGLEENIPP
                     MRNALKTLTKFSGSGKGVTISFNAELLGERLIWDELNDTLTIKGLPANLRDQLERNK"
     gene            2876..3217
                     /gene="bamE"
                     /locus_tag="PWY43_000842"
     CDS             2876..3217
                     /gene="bamE"
                     /locus_tag="PWY43_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDL2135740.1"
                     /translation="MKMKSLLAAMLLALGVTACSTVEKVVYRIDVPQGNYLEQDKIAQ
                     LKVGMTAEQVEYLLGTPMLKNIFETHRWDYVFIKREGYKDPEQRNLFIYFDAKGLVSK
                     IDDSNANAEQK"
     gene            3410..4750
                     /gene="gdhA"
                     /locus_tag="PWY43_000843"
     CDS             3410..4750
                     /gene="gdhA"
                     /locus_tag="PWY43_000843"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL2135741.1"
                     /translation="MSDLNALFQKIKQRDPNQAPFHQAVEEVFGSLAPFLAKNPKYTQ
                     QGLLGRIVEPERVISFRVTWVDDKGQVQVNRGYRVQMNSAIGPYKGGIRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADTD
                     VPAGDIGVGGREIGYMFGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFAES
                     MLATKGQQIEGKSVVISGSGNVAQYAAEKVIQKGGKVLTVSDSNGYVLFPESGMTEAQ
                     LQALLELKNERRERLSVYATEQGLKYFDGQKPWGVKCDIALPCATQNELDTEDAKELV
                     KNGCICVAEGANMPTTLGGVEVFIGAKILYAPGKAANAGGVATSGLEMSQNAIRLSWS
                     REEVDQRLFNIMKAIHENCVENGTEADGFVNYVNGANIAGFKKVANAMLEQGIV"
     gene            4912..7041
                     /locus_tag="PWY43_000844"
     CDS             4912..7041
                     /locus_tag="PWY43_000844"
                     /inference="COORDINATES: protein motif:HMM:NF024808.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2135742.1"
                     /translation="MIITMKDIAKEANVSLGTVSNVLNGKSNVSLAKIEKVQAAVEKL
                     GYRKNIQASTLKSGTSNKIAIILPNITELKYACLYENLDLICTQNDLSLILYLTHNRE
                     DREKQIIQNIKQDNYLRIILDSCLNNADYYYDEVKDQDKFIFVYRYLKNAKHFIGFDY
                     LPVIGDLIKHMKAHHLENIAFIKDNNCDWGGTVCDYLASYSQKNNIKILFFTQKESIH
                     KLSFELAQSIHRFDGIIVPSFNSAKILYSSFCLSNKIPPKIYTCYKNSFNNDDRFFQY
                     RLDYDFISNQILSILREDNAIIKEHHFGFPNIIESSNNTLPHQEINLLAVQTPSTESL
                     RKLLPYFEMLTGIQVNIDTYSFEQIPAVLENDQVEKKYDLVRIDMESLPYFAEKYFLP
                     LNSIGREHLELCFSASIIERFCLFQDKLYAIPFDPSIQMLFYRDDIFNDVKVKRLFYE
                     KNKKELSLPNNFEQFNQLSDFFKNHLPEEFGVKYGSALIVNDEGTLASEFLMRYYALS
                     DSIFMENVLKLEMQSAKKALKQLKAFYQSAKLLEQGWWKDAVELFNLGEIPMLIVYMN
                     HFLPLSHKGLMLPLGVSTLPNNRALMGGGSLAIMKQTQKIAACETFFNWFLDNTTQEQ
                     YVQLGGSSAKRDIVENQNITKYLPWLPLALKGDFSGVRENSNSRQEAINLRQAENIIG
                     GVVNVYLTTDMNEVEVVNLINARLSQL"
     gene            complement(7087..8253)
                     /locus_tag="PWY43_000845"
     CDS             complement(7087..8253)
                     /locus_tag="PWY43_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2135743.1"
                     /translation="MFNVSRLLIMMFLQYFTQGAWNMTIGAVLADYGMKNSIGSTYAL
                     LGLATLISPLFIGMIADRFLAAQKTIAILHLLNSVILYQIPSTILHNEYSQFFMLIFI
                     VGLLYYPTTALSNSITFQHLSDSRNFPFIRIFGNIGFITAGLIMGYFNIFDQVIVFEI
                     AAFSSLICGLYCFTLPNTPPAARHSVINWKTLLCLDAFILFRDKFFTIFMAVTMLLML
                     TKTSYSAYISVYLQDFQLNAANMMQIAVISEVIFMLLLSLSIKKFGFKWVMGMGIIAW
                     FIRTYLLSIAATATTPLTYIVISLALQGVCWTFFFATGDVYINTRQMIIFERKHKVYA
                     SCFLTILECWLPLILSALSIIMLSRSINCLSLLSNGQNFGYIQVRLHWLPVFYF"
     gene            complement(8329..9387)
                     /locus_tag="PWY43_000846"
     CDS             complement(8329..9387)
                     /locus_tag="PWY43_000846"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2135744.1"
                     /translation="MFMQFDINEHPHRRYNPLKDEWILVSPHRAKRPWSGQVDKAPTD
                     ELPSYDPTCFLCPNNNRVSGEQNPDYKGTFVFSNDFAALMPDSPNSPETSHPLLKIQG
                     VQGLARVICFSPDHSKTLPELPITAIKGIVDTWNEQLEELSKDYVWVQVFENKGAMMG
                     CSQPHPHGQIWANSFLPNEIEKKDKNLVRYYQKNGSNLLLDYAQLEMNDGQRTVIETE
                     HWIAVVPYWATWSFETMLMPKKRHIKRMNELTESEKQDLTYAIKLLTTRYDNLFECSF
                     PYSMGWHFAPFFKDGRDIEHWQLHATFYPPLLRSATVRKFMVGYEMLAETQRDLTPEQ
                     AAQRLASLSDVHYKQKEN"
     gene            complement(9458..10876)
                     /gene="uhpT"
                     /locus_tag="PWY43_000847"
     CDS             complement(9458..10876)
                     /gene="uhpT"
                     /locus_tag="PWY43_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDL2135745.1"
                     /translation="MAGFFDIKKIPPIGLTTAQQRKLWLRQFLKAFMVVFLAYMAMYL
                     IRNNFKAAQPFLKEQYGMSTLELGYIGLAFSIVYGIGKTALGYFIDGKNTKRIISALL
                     IGSSIAVMLMGFILSAFGSVVGVFIVLWGLNGVFQSAGGPASYSTIYRWVPRTRRATA
                     MGLRNSSHNIGGAIAGGLALWGANTFFAGNIAGMFIFPAVIALIIGIVGLYWGKDDPE
                     ELGWNRSEEIFEEPIEEENAAAETMTKWEIFKKYLLSNPWVWVLCVANVFGYILRIGI
                     DNWAPLYVRETLHFNDFDAVNTILWFEAGAFIGSLFWGTLADFFKGRTAAVGTVCLAI
                     VFFVIDFYRDGTSVMAVNIALFALGAMIFGTLTLIPLSIINLAPKRGVSVANGMAGTF
                     GYLFGDSTAKVGLALIADPKREGLTVFGHNLHGWRDVFIVFNISCVIAVVLLFLVALV
                     EERKIRSIQKAKELGLKVAKVD"
     gene            complement(10903..11874)
                     /locus_tag="PWY43_000848"
     CDS             complement(10903..11874)
                     /locus_tag="PWY43_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HDL2135746.1"
                     /translation="MKVIKTVATTLLLSLFSLSSFADSATQKILDNFQQYPFSQITVT
                     HRAQAGVESRIFPENSLAAINLAINRGVGIIEIDPRLTADGHYVLLHDETLDRTTNVE
                     EVFPQYKEKDGQYAGQVLLSKLTLEQVKQLKLTDGFDGQIHQVPTLKEVLAAAKGRVF
                     LDLDLKEIDVEKLSALIKEFGNDNLLAYNSNAELLKQVTDKTGVIPLPINTPITKDGD
                     PIADFHKFQKMWGDDFKIMHTFMSDVTPELVALADKHKVRLWINTLNDADGAAERFDY
                     SLWKSYLGSGANVFQSDFSIELTRFIELRNSCLTLKNVQGCEVFTEH"
     gene            complement(12186..13856)
                     /gene="glnS"
                     /locus_tag="PWY43_000849"
     CDS             complement(12186..13856)
                     /gene="glnS"
                     /locus_tag="PWY43_000849"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDL2135747.1"
                     /translation="MSEEILTAETGTRANFITHIIDEDLASGKHNNVYTRFPPEPNGY
                     LHIGHAKSICLNFGIAQDYNGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEP
                     RYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRSVEENL
                     ALFEKMKNGEFAEGKACLRAKIDMASPFIVMRDPVLYRVKFAHHHQTGDKWCIYPMYD
                     FTHCISDAIERITHSLCTLEFQDNRRLYDWVLDNISIERPLPHQYEFSRLNLEGTLTS
                     KRKLLKLVTDGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSAL
                     EACIREDLNENAPRAMAVINPVRVVIENFGEKEILKAPNHPNRPELGERDLPFTREIY
                     IDEADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGRISTIFCTYDPDTLGK
                     NPADGRKVKGVIHWVSAEDNKPAEFRIYDRLFTVPNPGAEEDINAVLNPESLVVKHGF
                     VESSLAHAKPEQGYQFEREGYYCLDSKDGSADNLVFNLTVSLKESVAF"
     gene            complement(14017..14460)
                     /locus_tag="PWY43_000850"
     CDS             complement(14017..14460)
                     /locus_tag="PWY43_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL2135748.1"
                     /translation="MNLQQIQDRLALKDLVDTFSNLADEKKVAEQMPLFTQDAVVNTY
                     IGGKLVFEMHGVAEIEQVFTTFLTPFHSVYHLNGQHTVTFIDENNATAINYCAVKLVE
                     TKEGKEILHDHSVRYADTYVKQEGKWRIAKRIANFMISESREIRQ"
     gene            complement(14511..15086)
                     /locus_tag="PWY43_000851"
     CDS             complement(14511..15086)
                     /locus_tag="PWY43_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL2135749.1"
                     /translation="MKQILILGASGSLANVVIPELLKDNNVQLTLFARRPQSVAKFET
                     ERVKIVQGDVLDFHALTQAMQGVDAVYAGLAGDLKAMAGNVVNAMNEAQVKRLVWISS
                     MGIYGETGEDHGAILEPYRQSAAVIEASELDYTVVRPGWFTNGSEVQYQLTHKGEPFK
                     GHSVSRKSIADFVARILRENSEIRESVGIAA"
     gene            15193..16083
                     /locus_tag="PWY43_000852"
     CDS             15193..16083
                     /locus_tag="PWY43_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2135750.1"
                     /translation="MKENLNDLRAFLLVAQTSSFTKAAAQMGVSQSALSHSIRGIEER
                     LQIKLFHRTTRSISTTEAGERLYQRLLPLFDEIDQEVNGLSEFRNALKGGLRINGNEH
                     VFRDVLADKFAEFVKRYPEVALELVAEDRFVDIVAERFDAGIRLGADVAKDMIALRIS
                     PDVKMTAAASPNYLAQHGTPKTPFDLTEHHCLKHRLATLGGILAWEFRDPITKKIVKF
                     QPQGNLTANNGFLLQHYAKQGLGILWSPQDSIQTELDNGSLVPILTDWAMQYEGYHLY
                     YPNRRQNSPLLKALIDVLRG"
     gene            complement(16150..16716)
                     /gene="efp"
                     /locus_tag="PWY43_000853"
     CDS             complement(16150..16716)
                     /gene="efp"
                     /locus_tag="PWY43_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL2135751.1"
                     /translation="MASYSTNDFKKGLKFIQDGEPCVIVENEFVKPGKGQAFTRTKIR
                     KLISGKVLEINFKSGTSVEAADVVDSNYNYSYSDGDFWYFMHPETFEQISVDEKALGD
                     NVKWLVDNAECIVTLWNGSAIAVTPPNFVELEIVETDPGLKGDTAGTGGKPATLSTGA
                     VVNVPLFVQIGEVIRVDTRSGEYVSRVK"
     gene            16883..17452
                     /locus_tag="PWY43_000854"
     CDS             16883..17452
                     /locus_tag="PWY43_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135752.1"
                     /translation="MKKLITLSAILAVSVATVAEAKRGGGSFRSVSPSKAVATKPVAS
                     QKSTTNQQTQQKDATFANTPNSATAQPQVRQGSTMANVAMGAAAGYILGDMMSSNAHA
                     NQPSQPADANAQAVNSAQNVATGAVPAIAEFKSIGGQIDPFLVEKTDGYRRYCISGVQ
                     YLVAAQGVQTSPTVMVNPNGTPLQCNLIP"
     gene            17506..18498
                     /gene="epmB"
                     /locus_tag="PWY43_000855"
     CDS             17506..18498
                     /gene="epmB"
                     /locus_tag="PWY43_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDL2135753.1"
                     /translation="MQPIQIKPRWLTELAQAFNNPIDLLQFLELNPNDFEGDIAARKL
                     FALRVPRMFAEKMEKGNPNDPLFLQAMSLQAEFIEAEGFVVDPLEEQQSPAPNILHKY
                     HNRLLFMLKNSCAINCRYCFRRHFPYEEVKSGKAVWQQGLTYIAEHPELEEVILSGGD
                     PLMAKDQDLDWILTQLEQISHIKTLRIHSRLPVVIPNRITTELCERLSKSRLNVVLVT
                     HINHANEIDAVFANKMAQLKKAGVVLLNQSVMLKGVNDNAQTLKRLSDKLFEYGILPY
                     YLHLFDKVAGASHFYIEDEQAGEIYRELQRITSGYLVPKLAREIAKEPNKILKG"
     gene            complement(18598..20001)
                     /gene="asnS"
                     /locus_tag="PWY43_000856"
     CDS             complement(18598..20001)
                     /gene="asnS"
                     /locus_tag="PWY43_000856"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL2135754.1"
                     /translation="MSKFPTVSEILSGKIAVGEEATVRGWVRTRRDSKAGLSFLSVYD
                     GSCFDPIQAIINNDLPNYNAEVLRLTAGCSVVVTGKVVESPAEGQAVELQASKVEVLG
                     WVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHCLAQAIHRFFHEQGFYW
                     VATPVITASDTEGAGEMFRVSTLDLENLPRGENGKVDFNQDFFGKESFLTVSGQLNAE
                     TYACALSKVYTFGPTFRAENSNTTRHLAEFWMMEPEVAFADLNDNAKLAEDMLKYVFK
                     AVLAERKDDMEFFVKHVDKEAISRLENFINSPFAQVDYTDAIEILLKSGKEFEFPVSW
                     GIDLSSEHERFLAEEHFKSPVVVKSYPKDIKAFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEVLDQRMNEMGLNPEDYWWYRDLRKYGTVPHSGFGLGFERLIVYVTGLQ
                     NIRDVIPFPRAPRNANF"
     gene            20404..21951
                     /gene="sbcB"
                     /locus_tag="PWY43_000857"
     CDS             20404..21951
                     /gene="sbcB"
                     /locus_tag="PWY43_000857"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDL2135755.1"
                     /translation="MSDKFSFFFYDYESFGVSPALDRPAQFAGIRTDADFNIIGEPVM
                     FFCKQTPDYLPSPEAVMVTGITPQQCNAEGVSEPEFAARIHAEFSRANTCTIGYNNIR
                     YDDEMTRYTFFRNFYDPYEYSWKNGNSRWDLLDLVRACYALRPDGIEWPTDETGMPSF
                     KLENLTKANGIAHENAHDAMSDVYATIAMAKLIKQKQPKLFDFFFNLRSKKEVEKLID
                     TGEMTPLVHVSGMLGNYRGNTAWVVPLAWHSTNQNAVIVCDLSGDMNGLLTENAEVLR
                     QRLYTKRDELAENELSVPLKLVHINKCPILAPAKTLLPENAERLGIDRALCLENLKTL
                     KAQKSLIREKVIEIFNEERTFEPSTNVETTLYDGFFSPADKNNMAILRTLPPEDLANH
                     GLKFEDPRVEALLFHYRARHYPQTLSRAEQIKWQKHCNQQIEAKAEQFAQSIDDLFQQ
                     HHDNPEKVKLLENLTAYAEQISQQQAVIYRQNVAKDEKLLSELNRVAEQPLDKTEKLK
                     MLKELIK"
     gene            21948..22547
                     /locus_tag="PWY43_000858"
     CDS             21948..22547
                     /locus_tag="PWY43_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDL2135756.1"
                     /translation="MTMDNLPLEQHPFDPILPEQATVVMVGTFPPTSEKRCMEFHYPN
                     FQNDMWRIMGNIFYADPNYFRVGEEKRFDPIRIEEFLREKGIALCSSAKTAIRLKGNA
                     SDKDLKIVDPIDMDELLLKLPKLKWLFTTGGLATDTLLGLLPEKHKAPKTNEWVVYPY
                     SATRELYLYRLPSTSRAYPLSLANKTEAYRQFFRTAGLI"
     gene            22559..>23151
                     /locus_tag="PWY43_000859"
     CDS             22559..>23151
                     /locus_tag="PWY43_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL2135757.1"
                     /translation="MKYDLHAHSTASDGVLSPTELVQRAVEQGVTMLALTDHDTISGI
                     QEAKNFAQNQPLELISGVEISILWQEKSIHLAALNIDENNEELVKFLDNQAQLREERA
                     VEIGEKLAKVGIANAYEGAKALATGEVTRAHYGRFLYEQGYVRNIEHAFKRYLGMGKP
                     AYVKPRWSSLEDAVNVTHTSGGVISIAHPLRYKMTAR"
BASE COUNT         7013 a         4548 c         4765 g         6825 t
ORIGIN      
        1 ctcatttgcg gatagctacc gattctattt tttgtgcttc gcttgcctgt tttggggctg
       61 atatagctat aaatccatac tttcgctttt ttctgtgcaa gaataaatag cccgttactg
      121 tctgcgatag gcttcgcatt tggggaatat ttaagatttt ttagtaatgt gtctgaaagt
      181 gtttttttag ttgcagtcat ttcttctaat ccttgatata taagggttta tcgaagttta
      241 tgatccctaa attttcccgt tttcaaaata gggatcattt tagggatcat aaacgctaag
      301 atacaatgca actaaagaag acaataagag actataaaat aatctaagtt cttgatttta
      361 tagtaaattt gagacaatga aagacaatat gcaacgctta aatggtcccc ccagccggac
      421 ttgaaccagc gaccaagcga ttatgagtcg cctgctctaa ccactgagct atggggggat
      481 ttgaaaagtg gttggaatta tagagaaaaa ccgagtagtc gtctagaggc agaagattac
      541 tcggttaatt attgaacgag agaaaatagg gttattctgt tcgccaaatt aaggttgcca
      601 ttcgcccggt ttgtttgtct cggcggtagg aaaaaaacag ttctggttgg ctgtaggtgc
      661 aatattcacc gcctgaaatg gcggtaatgc ctaatttatt cagacgttgg gtggcaattt
      721 ggtaaagatt tcctaaaaat ttaccgcttg tagtgctgtc ttcaataaag gcttctttcg
      781 ctcttgggtc aaaagcacag aattggtctg ccacttcact cccgacttga aaagcattcg
      841 gtccgatagc cggccctagc caagctgata tttcgtgtgg cgggcagttg aatttctcta
      901 ccgtcgcctc taatatgcca tcgcataacc cacgccagcc ggcgtgtgcc gccgcaattt
      961 ccgacccgtc ttggctggca aataataccg gcaaacaatc tgcagtcatc actaagcaga
     1021 cttggtttgg ttgattggtg taaatcgcat cggctttaat attatcacct gaatagggaa
     1081 gttcaatcac tcgggtgctg tgagtttggg ttaaaaacag cggagcttgt ggtaattgaa
     1141 atttctcaac caataaagca cgattttgag cgatatcttg tggctcgtca ttaacgtgat
     1201 cacctaagtt gaaactgtca aaaggcggtt tgcttactcc gcctgttctt aaggtcgtca
     1261 gtgcgtgaat atgtttaggc acattccaat ttggaataat tttatccatt tgcaaaaaat
     1321 ctcaagaaaa tgaccgcttg ttatcggcgt actgcaatcg cttcgatctc aatgccaaca
     1381 tcttttggta aacgagccac ttctacgcaa gaacgagccg gaaaattagg gtgttcgttt
     1441 tcttggaaga aggcttggta ttccgcattt acctttgcga agtcgttaag atctttgaca
     1501 aatacggtgg tctttacaat atcggcaacg gttaagccgg cttgttcgat aatggctttc
     1561 acgttgttca gagattggcg ggtttgttgc acaatatcac tcgggatttc gccggtttca
     1621 ggattcacgg gaatttgccc agaagttaaa accatattgc ctaaatcaac tgcttgcaca
     1681 taaggcccaa tcgctgccgg tgcattttct gtgtggataa ctgtcatctt gtgttcctct
     1741 taatgaaaaa taaacggatt aatgataata tcttttctta ttatttggaa taaagagaag
     1801 tgaattaatg agcattaatg tttctgaaat cgtgttacat caattacaaa aaacagaggg
     1861 cgagaacccg gagcttaata cgtttttgcg agaaaattta ttggcaattt cccctgaagt
     1921 cgatcaaatg atgttgcaat tacatcaagc ctatcaaagt aaagcaaagg cttacggtat
     1981 atttaaaccg gagtcggttt tcgctcaaca gctcaaccgc ttgttggagc aagaaaccga
     2041 ttttttacct tttagccata gctgtgccaa aatgcttgca gcagagctga ttaaatatcc
     2101 gtttgccgat ggtggcactt ttattttgtg tcgttacact tttttagcta ccgaatattt
     2161 gtttattgcg ttgattgata gccgtaattc aatgctggtt gatgagcagt tagagattaa
     2221 acgcacgcaa tatctcgaaa ttgcacaata tgatattgcc tgccgcatta atttaactga
     2281 actgaaactt gacgcaacgt caaatcgcta tttaactttt gtgaaaggca gagtagggcg
     2341 taaaattgcc gatttcttta tggatttttt aagtgcggaa gaagggttta atccacaatt
     2401 gcaaaatcaa accttgttgc aagcggtgag cgattattgt gagcaagggg agttatctgc
     2461 ccctcaaact cgagaagtga aaaagcaggt gtttgaatat tgtaaagggc agataaatag
     2521 tggagaggaa attgcgttaa atgagctttc caacgcattg ccaacactga atgaaatcga
     2581 ttttgcccaa tttacgcaag agcaagaata cggcttggaa gagaatattc cgcctatgcg
     2641 aaatgccctt aaaaccctga cgaaattttc cggttcgggc aaaggagtaa cgattagctt
     2701 taacgcagaa ttattaggag agcgtttaat ttgggatgaa ctaaacgaca cactgacaat
     2761 caaaggatta ccggcaaatc tgcgtgacca gctggaacgt aacaaataat ggaaaatttt
     2821 tatcagttta ggtattatct cgttatctga attttggatt atcaaaaggt caattatgaa
     2881 aatgaaatct cttttagctg caatgttgct tgcactgggt gtaacggctt gttcaaccgt
     2941 tgagaaagtg gtgtaccgta ttgatgtacc gcaaggcaat tacttagaac aagataaaat
     3001 tgctcaactt aaagtcggta tgacagcgga acaagttgaa tatttgcttg gtacaccaat
     3061 gttaaagaac atttttgaaa cccaccgttg ggattatgtg tttattaaac gtgaaggtta
     3121 taaagatcct gaacaacgca atctgtttat ttattttgat gctaaagggt tagtatcaaa
     3181 aattgatgac agcaatgcga atgcagaaca aaaataggac gatttgctgg ttcgaaaagc
     3241 ccacttttaa aagtgggctt atttttttga ataaaaaata agcatttaaa cttaattttt
     3301 cttattttaa tgataaattt ttctaatcta tcttttcaaa gctaaaattg cttgctagac
     3361 ttcgctcatc ttaaatacac aacatcatca atttattcag gagtataata tgagcgattt
     3421 aaacgccctt ttccaaaaaa tcaaacaacg tgatccgaac caagcccctt tccatcaagc
     3481 ggttgaagaa gtgttcggta gccttgcccc tttcttagct aaaaatccga aatataccca
     3541 gcaaggttta ttagggcgta ttgttgagcc tgagcgtgta attagcttcc gtgtcacttg
     3601 ggttgatgat aaaggtcaag tgcaagtaaa ccgtggttac cgtgtacaaa tgaactcagc
     3661 aatcggtcca tacaaaggcg gtattcgttt ccacccaacc gttgatttag gcgtattaaa
     3721 attcttagcc tttgagcaag tattcaaaaa tgcgttaacg acattgccaa tgggcggtgg
     3781 taaaggtggt tcagactttg acccgaaagg caaatctgat gccgaagtaa tgcgtttctg
     3841 ccaagcgttt atgagcgaat tatatcgcca cattggtgct gatactgacg ttcctgcagg
     3901 cgatatcggc gtgggaggtc gtgaaatcgg ctatatgttc ggtcaataca aaaaattacg
     3961 caatgaattt acttcagtat taaccggtaa atcgctgact tggggtggta gcttaattcg
     4021 tccggaagca actggttatg gtgcggtgta ttttgcagag tctatgttag cgactaaagg
     4081 tcagcagatc gaaggaaaat cagtggtaat ttccggttcg ggtaacgtgg cacaatatgc
     4141 agctgaaaaa gtgattcaaa aaggcggtaa agtattaacg gtttcagatt ctaacggtta
     4201 tgtcttattc cctgaatccg gtatgacaga ggcacaatta caagcattat tagagttgaa
     4261 aaacgaacgc cgtgaacgct tatctgtgta tgcaacagaa caaggcttaa aatactttga
     4321 cggtcaaaaa ccgtggggtg tgaaatgtga catcgcttta ccttgtgcaa cccaaaacga
     4381 attagatacc gaagatgcta aagaattagt gaaaaacggc tgtatttgtg tggcagaagg
     4441 tgcaaatatg ccaaccacat taggtggtgt tgaagtcttt atcggtgcga aaatccttta
     4501 tgctccgggt aaagcggcta acgcaggtgg tgtggcaacc tcaggtttag aaatgagcca
     4561 aaatgcaatt cgtctatctt ggagtcgtga agaagtagat cagcgtttat tcaacattat
     4621 gaaagcaatt cacgaaaact gcgtagaaaa tggtactgaa gcagatggtt tcgtaaacta
     4681 tgtaaacggt gcgaatattg ctggtttcaa aaaagtggca aatgcgatgt tagaacaggg
     4741 tattgtttaa tttattatcc ctattattca agaactgcct cgatatatcg aggcagtttt
     4801 tttattttag gttgtagcta gagacaagct aaattgaaat cgtatttcat ttgccaatta
     4861 tggtaaaatc tatgccaatc tcagttttag cagtatgtga aagggcagta tatgatcatt
     4921 acgatgaaag atatcgcaaa ggaagcaaat gtttcgttag gaacagtatc gaatgtatta
     4981 aacggaaaaa gtaatgttag cttagctaaa atagaaaaag tacaagctgc cgttgaaaag
     5041 ttgggttatc gtaaaaatat tcaagcctct acgttaaaaa gtggcacaag taataaaatt
     5101 gctattatat tgcccaatat tacagagctg aaatatgcct gtttatatga aaatcttgat
     5161 ttaatttgta cgcaaaatga tttatctctc attctttatt taacgcataa tcgagaagat
     5221 agagaaaaac aaattattca aaatataaag caagataact atttgcgtat tattttagat
     5281 agttgcctaa ataatgcaga ctattattac gatgaagtaa aagaccaaga taaatttatt
     5341 tttgtttatc gttaccttaa aaatgcaaaa cattttattg gttttgatta tcttcctgtg
     5401 attggtgatc ttataaagca tatgaaagct catcatctag aaaatattgc cttcattaaa
     5461 gataataatt gtgattgggg tggaacagta tgcgattatt tggcaagtta tagtcaaaaa
     5521 aataatatta aaatattgtt tttcacccaa aaggaatcca ttcataaatt atcgtttgaa
     5581 ttggctcaaa gcattcatcg gtttgatggc attattgtgc cttcatttaa ttctgctaaa
     5641 atcttatata gttcattttg tttatcaaat aagataccac ctaaaatata cacctgctat
     5701 aaaaatagct ttaataatga tgaccgattt tttcaatata gattagatta cgattttatt
     5761 tctaatcaga tcttatctat tcttcgagaa gacaatgcta tcataaaaga gcatcacttt
     5821 gggtttccaa atattataga aagctcaaat aatacactac ctcatcaaga aattaattta
     5881 cttgcagtcc agacaccatc aacagaatct cttagaaaat tattacctta ttttgaaatg
     5941 cttaccggga ttcaagtcaa tattgataca tactcatttg agcaaattcc tgcggtgctg
     6001 gaaaatgatc aagtagagaa aaaatatgat ctggtgcgaa ttgatatgga aagtctgcct
     6061 tattttgcgg aaaaatattt cttacccctt aatagtatag gaagagaaca cctagaatta
     6121 tgtttttccg caagtattat tgaacgcttt tgtctgtttc aagataaact ctatgctatt
     6181 ccgtttgacc caagcataca gatgttattc tatcgagacg atatttttaa tgatgttaaa
     6241 gtaaaaagat tgttttatga aaaaaacaaa aaagaattat ctttgccaaa taatttcgag
     6301 caatttaacc aactgtctga tttctttaaa aatcatttgc ctgaagagtt cggcgtaaaa
     6361 tatggctctg cgttaattgt caatgatgag ggaacattag cctctgaatt tttaatgcgt
     6421 tattatgcgt tatcagattc tatttttatg gaaaatgtac tgaagttaga aatgcagtcg
     6481 gctaaaaaag cgttaaaaca acttaaagcc ttttatcagt cagcgaaatt attggagcaa
     6541 ggctggtgga aagatgcggt tgaattgttt aatttaggcg aaattccaat gcttattgtg
     6601 tatatgaacc atttcttacc attatcacat aaagggttaa tgttgccact tggcgtttct
     6661 acattaccga ataatcgagc attaatgggg ggcggctcat tagccataat gaaacaaaca
     6721 cagaaaatag cagcctgtga aacatttttt aattggtttt tagacaatac cacccaagaa
     6781 caatatgttc agttaggggg cagttcagct aaaagagata ttgtagaaaa tcagaatata
     6841 actaagtatt tgccttggtt acctttagca ttaaaaggcg atttcagcgg tgtaagagag
     6901 aatagtaata gtcgtcaaga agcgattaat ttaagacaag ctgaaaatat tataggtggt
     6961 gttgttaatg tgtatttaac cactgatatg aatgaagttg aggtggtgaa cttgattaat
     7021 gcaaggttaa gccagctatg aataaaacag cttgctatta ggtcgccttt tcatttttaa
     7081 atagcgttaa aaataaaata ccggcaacca atgcaatcgc acttggatat aaccaaaatt
     7141 ctgaccattg ctcagcaatg agaggcagtt gatgctgcgt gataacatta ttatagataa
     7201 tgccgataat ataagaggca accaacactc caatatcgtt agaaagcatg aagcgtaaac
     7261 tttgtgcttg cgttcgaata tgatcatttg ccttgtatta atatagacat cgcctgtggc
     7321 aaagaagaat gtccaacata ctccttgtaa tgccaagctt ataacgatgt aagttagcgg
     7381 tgttgtagca gttgcggcaa tcgacagtaa ataagtgcga ataaaccaag caataatgcc
     7441 catccccatt acccatttaa agccaaattt cttgatactt aagctaagca atagcataaa
     7501 aataacttct gatataactg ctatttgcat catattggcg gcattgagct gaaagtcttg
     7561 cagataaacc gaaatataag cactataaga ggttttagtc agcattaata acatcgttac
     7621 cgccataaaa atggtaaaga acttatctcg gaatagaata aatgcgtcta gacataataa
     7681 ggttttccag tttattacgc tatgacgggc agccggcggt gtattcggta aggtgaaaca
     7741 atataagcca catatcaacg atgagaatgc tgctatttca aaaacgatca cttggtcaaa
     7801 aatattgaaa tagcccataa taagtccggc agtaataaag ccaatattgc caaaaatacg
     7861 aatgaatgga aaatttctac tgtcagataa atgctgaaat gtaatgctat tagaaagtgc
     7921 ggttgtgggg taatagagca atcccactat aaaaatcagc ataaagaatt gagaatattc
     7981 attatgtaat atagttgagg gaatttgata caaaatcaca ctgttcagta ggtgtagaat
     8041 agcgatagtt ttctgtgctg ccaaaaatct atcggcgatc attccaataa atagcggtga
     8101 aataagggtt gcaagtccaa gtaaagcata ggtagaacca atgctgtttt tcattccata
     8161 atctgctaat accgccccaa tggtcatatt ccacgcccct tgggtgaagt attgcagaaa
     8221 catcataatt aaaagtcttg aaacattgaa catatcaacc gtattttcta aaggaggaat
     8281 atatttagtg gctaaaattg ttatctaaat atattccgtg ttgaaaaatt aattttcttt
     8341 ttgcttatag tgaacatcgc tcaaagaggc taaacgctgt gcagcttgct caggtgtgag
     8401 gtctctttgg gtttcggcta acatttcata acctaccata aatttacgca ctgtcgcaga
     8461 gcggagcagt ggcggataga atgtagcgtg cagttgccaa tgttcaatat ctcgtccatc
     8521 tttaaagaat ggagcaaaat gccaccccat agaataaggg aaagagcatt caaataagtt
     8581 atcataacga gtcgtgagta atttaatcgc ataagttaaa tcctgttttt ctgactcggt
     8641 aagttcattc attcttttaa tatggcgttt tttcggcatt agcattgttt caaacgacca
     8701 cgtagcccaa tagggtacaa ctgcgatcca atgttctgtt tcgatcaccg ttctttgacc
     8761 atcattcatc tcaagctgag cataatctag caaaagattt gagccatttt tctgataata
     8821 gcgaactaaa tttttatctt ttttctcaat ttcgttaggt aaaaaagaat ttgcccaaat
     8881 ttgcccgtgt gggtgaggct gggaacagcc catcattgcc cctttatttt caaatacttg
     8941 cacccataca taatctttgc ttaattcttc aagctgctca ttccaagtat caacaatgcc
     9001 tttaattgcg gtaataggta attcaggcaa ggttttgctg tgatcaggcg aaaagcagat
     9061 gactctcgcc agcccttgaa caccttgaat cttaaggagt gggtgagatg tttccggcga
     9121 gttgggcgaa tccggcatta aggcggcaaa atcattgctg aatacaaagg tgcctttata
     9181 gtccggattt tgctcccctg aaactcggtt gttattcggg cataaaaaac aagtgggatc
     9241 ataactcggt aattcatcag tcggtgcttt atccacctgc ccgctccaag ggcgtttggc
     9301 tcggtgtgga gagactaaaa tccactcatc tttcaacgga ttataacggc gatgagggtg
     9361 ttcattaata tcgaattgca taaacatatc ctgattataa tagacggaac agaccgcttg
     9421 cctaaacaag cggtcatttt tcattgaaaa tttgcaatta atctactttt gcgactttta
     9481 atcctaattc ttttgctttt tgaatactac ggattttacg ttcttcaaca agagcaacaa
     9541 ggaataaaag taccacggca atcacgcaag agatgttaaa gacgatgaat acatcacgcc
     9601 aaccgtgtaa attatgtccg aatacggtta aaccttcacg ttttggatct gcaattaacg
     9661 ctaaaccgac ttttgcggtg gaatcgccaa ataagtaacc aaatgtacct gccataccgt
     9721 ttgcaacgct tacccctctt ttcggtgcta agttaataat tgaaagtggg attaaggtta
     9781 aagtaccgaa gatcatcgca cctaaggcaa ataatgcgat atttacagcc attacacttg
     9841 tgccgtctcg gtagaaatcg atcacaaaga acacaatcgc taggcagact gtacctactg
     9901 cggcagtacg acctttgaag aagtcggcta atgttcccca gaataagcta ccgatgaatg
     9961 ctccggcttc aaaccataag atagtgttta ctgcatcgaa atcattgaag tgtaaggttt
    10021 cacgaacgta gagtggtgcc cagttatcaa tcccgattcg taaaatataa ccgaaaacat
    10081 ttgccacaca aagcacccaa acccaagggt tagacaacag gtatttttta aagatttccc
    10141 attttgtcat tgtttcggct gccgcatttt cttcttcgat tggctcttca aagatttctt
    10201 cactgcgatt ccagcctaac tcttccgggt catctttacc ccaatacaag ccaacaatac
    10261 caatgataag tgcgatcacc gcagggaaaa taaacatacc tgcaatgtta ccggcaaaga
    10321 aagtattggc tccccatagg gctaaaccac cggcaatagc accaccgata ttatgcgagc
    10381 tattccgtaa acccattgcg gttgcacgtc ttgttcttgg tacccaacga taaattgtgc
    10441 tataggaagc agggcctccg gcagattgga acaccccgtt caatccccat aacacaatga
    10501 atacacctac aaccgaacca aaggcactta agataaaacc cattagcata acagcaatag
    10561 atgatccaat taataaggca gaaataatgc gtttggtatt tttgccgtca ataaagtagc
    10621 ctaacgctgt tttaccaatc ccatatacaa tcgagaaagc caagccgata tagcccaact
    10681 caagagtgga cataccatat tgttctttta agaaaggttg tgcagcttta aagttattgc
    10741 gaattaaata catcgccata taagctaaga ataccaccat aaaggctttc aagaactgac
    10801 gtagccatag cttacgttgt tgtgctgtgg tcaaacctat tggtgggatt tttttaatat
    10861 caaagaagcc agccatataa actccaaatt atcggttaaa gattaatgtt cggtaaatac
    10921 ttcacaacct tgtacatttt tcaaggttaa gcaagaatta cgcaattcaa taaaacgggt
    10981 taattctata gaaaagtcgg attggaatac gtttgctcca gagcctagat aacttttcca
    11041 taaggaataa tcaaatcttt cggcagcccc atcggcatca ttgagtgtat tgatccaaag
    11101 acgcactttg tgcttatcag ctaatgccac taattcaggg gttacatcag acataaaagt
    11161 atgcataatt ttgaaatcgt ccccccacat tttttggaat ttgtggaagt ctgcaatcgg
    11221 atcgccatct ttggtaatcg gggtattaat tggcagagga attactccgg ttttatctgt
    11281 aacttgtttt aaaagctcag cattgctgtt gtaagccagt aaattatcat tgccgaattc
    11341 cttaattaac gcagacagtt tctctacatc aatttctttc aaatccaaat caagaaatac
    11401 tctgcctttt gcagcggcaa gcacttcttt tagggttggc acttgatgaa tttgcccatc
    11461 aaaaccgtct gtcaatttta attgttttac ctgctctaac gtgagcttag aaagcaaaac
    11521 ctgaccggca tattgaccgt ctttctcttt atattgtgga aagacctctt ctacgttagt
    11581 ggttctatcc agcgtttcat cgtgtaataa aacgtagtga ccatcagcgg ttaaacgagg
    11641 atcgatttca atgatgccta cgccccgatt aatcgctaaa ttaatggcag ctagagagtt
    11701 ctctgggaaa atacggcttt ctacaccggc ttgcgctcgg tgggtgacgg taatttgaga
    11761 gaaagggtat tgttggaaat tatccaaaat cttctgtgtt gcggagtccg caaaactaga
    11821 tagtgagaat aaagatagta gcaaggtggt tgctactgtt ttgattactt tcatagtaac
    11881 tccttaatat agtttggcga ccatctcaaa ataattgaaa cgtttcaatt agagaaggtg
    11941 gattttgtga ctgagatcac aaatttaaac attggtctgt atttttgaga gattttgttt
    12001 gaaactgccg gcaaattcgc ttaaccttaa ggcaacaaga ataagtgttg tacctagcaa
    12061 cctaacacat aaatttgctt tccgagtggc cccaagaatt ggggcttttt ttatgcttga
    12121 tgagtcgttt tgagagtaaa aaaaatacca gcatatttgc tggtatttga atgaagagag
    12181 ttagattaga acgctacgct ttcttttaag ctgacagtta agttaaatac aaggttgtct
    12241 gccgaaccat ctttgctgtc taagcagtag taaccttcac gctcgaattg gtagccttgt
    12301 tccggtttgg cgtgagctaa acttgactca acaaaaccat gcttaaccac taatgattca
    12361 ggatttagca ccgcattgat gtcttcttcc gcccccgggt ttggtactgt aaataggcgg
    12421 tcgtaaatgc ggaactctgc cggtttgtta tcttcagcag atacccaatg aatcacgcct
    12481 tttactttgc gaccatcagc cggatttttg cccaatgtat ccgggtcgta tgtgcaaaaa
    12541 atagttgaaa ttcgaccgct tgcatctttt tccacacgct cagctttaat cacataggca
    12601 ttgcgtaaac gaacttcttt gcctaagact aaacgtttat attgcttatt ggcttcttca
    12661 cggaagtcgg cttcatcaat ataaatctca cgggtgaacg gtaaatcacg ctcgccaagc
    12721 tctggacgat ttgggtgatt aggggctttt aatatttcct tttcaccgaa gttttcaatc
    12781 accactcgaa ccgggttaat caccgccatc gcgcgtggtg cgttttcatt taagtcttca
    12841 cgaatgcacg cttcaagggc agagtattcc accacgttat cttgcttggt taccccaata
    12901 cggcgacaaa attcacgtaa agacgctggg gtataaccac ggcgacgtaa gccggaaatg
    12961 gttggcatac gagggtcatt ccaaccatca acaatgccgt ctgtgactaa ttttaataat
    13021 ttacgcttag aggttaaagt accttctaaa tttaaacgtg aaaattcgta ttggtgcggt
    13081 aacggtcttt caatcgaaat attgtccagc acccaatcat ataagcggcg gttatcttgg
    13141 aactctagcg tacatagcga gtgagtgatt cgctcaatcg catcggaaat gcagtgggta
    13201 aaatcgtaca tcgggtagat gcaccattta tcaccggttt gatgatggtg agcaaatttc
    13261 acacggtaaa gcaccggatc tcgcatcaca ataaacgggg acgccatatc aatttttgct
    13321 ctcaagcagg ctttcccttc cgcaaactct ccgtttttca ttttttcgaa taacgctaag
    13381 ttttcttcca cgctacgatc acggtaaggg ctgtttttgc ccggctcggt taaagtacca
    13441 cggtattcac gcatttcatc aggcgataat tcatcaacat aggctaatcc tttgttgatt
    13501 aactcaatcg catagccata aagctggtca aaatagtctg aggcataacg aggctcaccc
    13561 tcccatttaa aacctagcca ctccacatct tgcttaattg aatctacata ttccacgtct
    13621 tctttaaccg ggttggtatc atcaaaacgc aagttacata aaccgttata atcttgagcg
    13681 ataccaaaat ttaaacagat agatttagcg tgtccgatat gcaaataacc gtttggttca
    13741 ggtggaaaac gagtgtaaac attgttgtgc tttccgcttg ctaaatcttc atcaataata
    13801 tgggtaataa aattggctcg agtgccagtt tctgccgtta aaatttcttc gctcatagtt
    13861 ctctctaaat gtatcaataa aaataacgga ttattctaca cttaattgaa acagacttaa
    13921 agactaaagc aaaattcctc ggtttcctcc aaagaatttt gctgttttag taagatgcaa
    13981 tggaattgca aatttttagc taaatttaac cgcttgttat tgcctgattt cacggctttc
    14041 gctgatcata aaattcgcaa tccgtttggc aatccgccat ttaccctctt gtttaacata
    14101 ggtatcggca taacgcacgc tgtgatcgtg caagatctct ttgccctctt tggtttctac
    14161 cagtttcacc gcacaatagt tgatcgctgt ggcgttattt tcatcaataa aagtcacggt
    14221 atgctgaccg tttaaatgat aaacgctgtg aaacggggtt aaaaaggtgg taaacacctg
    14281 ttcgatttct gccacgccgt gcatttcaaa caccaatttt ccgccgatgt aggtgtttac
    14341 gacggcatct tgcgtgaaaa gcggcatctg ctcggcaacc tttttttcat cggcaaggtt
    14401 agagaatgta tctactaaat cttttagtgc taaacgatct tggatttgtt gtaagttcat
    14461 tgtgttttcc ttctgatttt tgcaaatttt ctttggtttt tcaccgcttg ttaggctgca
    14521 ataccaacac tttcacggat ttcgctgttt tcacgcaaaa tgcgagccac aaaatcggca
    14581 atggatttgc gggaaacgct gtgtcctttg aacggttcgc ctttgtgagt cagctgatat
    14641 tgcacttccg aaccgttggt aaaccagccc gggcggacaa cggtataatc caactcgctg
    14701 gcttcaatca cggctgccga ttggcggtaa ggctctaaaa ttgccccgtg atcttcgccg
    14761 gtttcgccat aaatccccat tgagctaatc caaaccaagc gttttacctg agcttcattc
    14821 attgcgttca ccacatttcc cgccatcgct tttaagtcgc cggcaagtcc agcgtaaacg
    14881 gcatcaacgc cttgcattgc ttgggttaat gcgtggaaat ccaacacatc gccttgcaca
    14941 attttgacgc gttcagtttc aaatttcgct actgattgcg gacgacgggc aaaaagggtt
    15001 aattgcacat tattgtcttt tagcagttca ggaatcacga cattcgctag gctaccgctt
    15061 gctcctaaaa ttaagatctg tttcattgct gttctccatt gaatgaagtc agtatagcgg
    15121 tgtagattgc atttgattag tagggtaaaa tcgcttgaag taatgagaaa aattcattaa
    15181 taaggtggtt aaatgaaaga aaacctcaac gatttaagag cttttttgtt ggtagcacaa
    15241 acgagcagtt ttaccaaagc agcagcacag atgggcgtgt cgcagtcggc gttgtcgcac
    15301 agcattcggg ggattgaaga acggttgcaa atcaagctgt ttcatcgcac cactcgtagc
    15361 atttcaacca ccgaggcagg ggagaggctg tatcaacggc tgttaccgct gtttgatgaa
    15421 atcgaccaag aagtaaatgg gctgagtgag tttcgcaatg cgttaaaagg cgggctgaga
    15481 attaacggta atgaacacgt tttccgtgat gtgctggcag ataaattcgc tgagtttgtc
    15541 aaacgttacc ctgaagtagc attggagcta gtggcggaag atcgctttgt agatattgtc
    15601 gccgagcgtt ttgatgccgg tattcggctt ggggcagatg tggcgaaaga tatgattgca
    15661 ctacgcattt cgcccgatgt gaaaatgacc gcagctgcca gccccaacta tttggcacag
    15721 cacggcacgc cgaaaacgcc ctttgattta accgaacatc attgtctcaa acatcgtctt
    15781 gcgacattgg gcggcattct ggcgtgggaa tttcgggatc ccatcaccaa gaaaatcgtt
    15841 aaatttcaac cgcagggcaa tcttactgcc aataacggct tcttgctgca acactatgcc
    15901 aaacaaggtt taggcattct ttggagtccg caagacagca ttcaaaccga gctggataac
    15961 ggcagcctcg tgccgatttt aaccgactgg gcaatgcagt atgagggcta tcatctctat
    16021 taccccaacc gtcgccaaaa ttcgcctttg ctgaaagcgt taattgatgt gctgagagga
    16081 taggtaaaaa tgagcggtcg tttttagcaa aaaattgacc aaaaaacgac cacttgtaga
    16141 gaagctaaat tatttcacac gagaaacata ttcgcctgaa cgggtatcca cacggataac
    16201 ttcaccgatt tgaacgaata aaggcacgtt taccacagca ccggtgctta atgttgccgg
    16261 tttaccgcct gtacctgcag tatcgccttt taagcccggg tcggtttcga cgatttctaa
    16321 ctcaacgaag tttggtggtg tgactgcaat tgctgaaccg ttccataaag tcacgataca
    16381 ttctgcatta tcaactaacc attttacgtt gtcgcctaac gctttttcat ctaccgagat
    16441 ttgttcaaag gtttcagggt gcataaagta ccagaaatca ccatcgctgt aagagtagtt
    16501 gtagttagaa tctactacat ctgctgcttc aaccgaagtg cctgatttga agttgatctc
    16561 taatactttg ccagagatta atttacggat tttagtacgg gtaaatgctt gacctttacc
    16621 cggttttacg aattcgtttt caacaattac acaaggttcg ccatcttgaa taaattttaa
    16681 acctttttta aagtcgttag tgctgtagct agccattatt tcctcaatta tttaaaaaat
    16741 aacaataaat taacaaattt tgaacccaac cctattgggt ttgtcaaaaa gagccatata
    16801 ataacctaaa ttcacaaaat tagcgaaaga tctcttgcga tttttgctat aatttttcaa
    16861 actttattga taaggaatgc gaatgaaaaa actgattact ctttctgcga ttttagccgt
    16921 ttctgttgca accgtggcag aggcaaaacg tggcggtggg agcttccgtt cagttagccc
    16981 gtcaaaagcg gtagcaacta agccggtagc ttctcaaaaa tcaaccacaa atcagcaaac
    17041 gcaacaaaaa gatgctactt ttgccaatac gccaaacagt gcaactgccc agccacaagt
    17101 gcgtcaaggc agcacgatgg caaatgtagc gatgggggca gcagccggtt atattttagg
    17161 tgatatgatg tcatcaaatg cacatgcaaa tcagcctagc cagcctgcgg atgcaaatgc
    17221 acaagcggtc aattctgcac aaaatgttgc aactggagca gttcctgcta ttgcagaatt
    17281 taagagtatt ggcggtcaaa tcgatccgtt tttagtagaa aaaaccgatg gttatcgccg
    17341 ttattgtatt tcaggcgtgc aatacctagt cgctgcacaa ggtgtacaaa cctcaccaac
    17401 tgtaatggtt aatccaaatg gcacgccatt acaatgtaac ttaattccat aattattttt
    17461 atttgcgggc aggcaaaaac ctgccccttg tatctacaat attcaatgca accaattcaa
    17521 atcaaaccgc gttggctaac cgagttggct caagccttta ataaccctat cgacttatta
    17581 caatttcttg aactcaatcc aaatgatttt gagggcgata tagctgcccg taaattattt
    17641 gctcttcgag tgccaagaat gttcgccgaa aaaatggaaa agggtaaccc aaacgatccg
    17701 ctttttttgc aagcaatgtc tttacaggcg gaatttattg aggcagaagg gtttgtggta
    17761 gatccattgg aagagcaaca aagcccagca ccgaatattt tacataaata ccataaccgt
    17821 ttgttattta tgctaaaaaa cagttgtgcg attaactgcc gctactgttt tcgccgtcat
    17881 ttcccttatg aggaagtgaa aagcggtaaa gctgtgtggc aacagggact aacttatatt
    17941 gctgagcatc ccgagctgga ggaagtgatt ctgtccggtg gtgatccgct gatggcaaaa
    18001 gatcaggact tagattggat attaacgcaa ttagagcaga tttcgcatat caaaaccctg
    18061 cgtattcatt cacgcttgcc ggtcgttatt cccaatcgaa ttacgacaga attatgtgaa
    18121 cggttatcaa aatctcgctt gaatgtcgtg ttggttaccc atattaatca cgccaatgaa
    18181 attgatgctg tttttgcaaa caaaatggct caactgaaaa aagccggcgt tgtattgctg
    18241 aatcaatcgg tgatgttaaa aggtgtaaac gataatgctc aaaccttaaa aaggttaagt
    18301 gataaacttt ttgaatatgg gattttgcct tactatttgc atttatttga taaagtggca
    18361 ggggcgagcc atttctatat tgaagatgaa caggctggtg agatttaccg agaattacag
    18421 cgtatcactt caggctatct tgtgccaaag ctggcaagag aaatagcgaa agagccaaat
    18481 aaaattttga aaggttagct taaacagaaa tcaagaaaca aaaggcgaaa atggtaaaat
    18541 tttcgccttt tttaaccgct tgttgcaagc ggttaaattc tcttactttt ttgcaaatta
    18601 gaagttcgca ttgcgtggag cacgtgggaa tgggattaca tcacgaatgt tttgtaaccc
    18661 ggttacatac acgattaaac gctcgaaacc taaaccgaaa cctgagtgtg gcactgtacc
    18721 gtatttacgc aaatcacgat accaccagta atcttcaggg tttaagccca tttcattcat
    18781 acgctgatct aacacttcta aacgttcttc acgttgagaa ccaccgataa tttcaccgat
    18841 tcccggagct aaaacgtcca ttgcggcaac cgttttgccg tcatcattta aacgcatata
    18901 gaaggcttta atgtcttttg gatagctttt cacgaccacc ggcgatttaa agtgttcttc
    18961 ggctaagaaa cgctcgtgtt cagaggaaag atcgattccc caagacaccg ggaattcaaa
    19021 ttctttgccg gatttcaata agatctcaat cgcatcggtg taatctactt gagcaaatgg
    19081 cgaattgata aagttttcta aacgactaat cgcttcttta tcaacgtgtt taacgaaaaa
    19141 ttccatatca tctttacgtt ctgcaagcac cgctttaaac acatatttca gcatatcttc
    19201 ggctaatttt gcattgtcgt ttaagtcggc aaaggcaact tccggctcca tcatccagaa
    19261 ttctgcaaga tggcgagttg tgtttgagtt ttctgcacgg aatgtcggac cgaaagtata
    19321 aactttactt aaggcacacg cataggtttc cgcatttaac tggccggata cggttaagaa
    19381 cgattcttta ccgaagaaat cttggttaaa atccactttg ccgttttcgc cacgaggtag
    19441 attttctaaa tccagcgtag aaacacggaa catttcacct gcaccttcag tatctgaggc
    19501 ggtaattaca ggtgttgcta cccaatagaa accttgctcg tggaagaaac ggtggatagc
    19561 ttgagctaaa cagtgacgaa cacgagcaac tgcaccgatt aagttcgtac gagggcgtaa
    19621 gtgagcgact tcacgcagat attcgataga gtggcgtttc gctgccattg ggtaggtatc
    19681 aggatcttcc acccaaccaa gcacttccac tttacttgct tgtaattcaa cggcttgacc
    19741 ttcagcaggg gattccacaa ctttaccggt caccaccaca gagcaacctg cggttaaacg
    19801 taatacttcg gcattataat ttggtagatc gttattaata atcgcttgaa ttgggtcaaa
    19861 gcaagaacca tcgtaaacgc tcaagaaaga taaaccggct tttgaatcac ggcgggtacg
    19921 cacccaacca cgcactgtcg cttcttcacc taccgctatc ttgccggata ggatttccga
    19981 aactgttgga aatttagaca ttgaatacct caaattcttt attgtgagtt ttaatataag
    20041 aaacgttccg tattctacaa gaaaataggg ggaaatataa taattttacg gaattatttg
    20101 caaatttgtg agtctatata agcattgcaa gtagttaaat ctgattttgc ttggtgttaa
    20161 aaaatttttt attttttaat gaactttcgt tcagttaggc agtctgagta tatgtaacag
    20221 atttatgcaa gtttttttca tatttcctta ggtaagacct ccgccccttt tcgctttttt
    20281 tgattagggg ctttttttta ttttagcatt tgcaaatttc taacggaaat tgaccgcttg
    20341 tattccctcc tttctgcctt actgtgctac aatcagccca attttttcta acaaattgaa
    20401 cgaatgagcg ataaattctc ttttttcttt tacgattatg aaagtttcgg cgtgagtccg
    20461 gcgttagatc gtccggcaca atttgccggt attcgaaccg atgcggattt taatattatc
    20521 ggtgaaccgg taatgttttt ttgtaagcaa acgccggatt atttacccag ccccgaagcg
    20581 gtgatggtta ccgggattac accgcaacaa tgtaacgctg aaggtgtatc tgagccggaa
    20641 tttgcagccc gaattcacgc tgaatttagc cgagcgaata cttgtactat cggttataac
    20701 aatattcgtt atgatgatga gatgacccgt tataccttct tccgcaattt ttacgatcct
    20761 tacgaatata gctggaaaaa cggaaattct cgttgggact tgttagattt agtccgtgcc
    20821 tgttatgcgt taagacctga tggtattgaa tggccaacag atgaaaccgg tatgccaagt
    20881 tttaaattgg aaaacctcac caaagccaat ggcattgccc atgaaaatgc acacgatgcg
    20941 atgtctgatg tgtatgccac catcgcaatg gcaaaattaa ttaagcaaaa acagccaaag
    21001 ttgttcgatt ttttctttaa tttacgcagt aaaaaagaag tagagaagtt aattgacact
    21061 ggtgaaatga caccgcttgt gcacgtttcc ggtatgctgg gcaactatcg aggaaatacc
    21121 gcttgggttg tgccactggc ttggcactca actaatcaaa atgccgtaat tgtgtgtgat
    21181 ttatcgggcg atatgaatgg cttattgact gaaaatgcag aggttttacg tcagcgttta
    21241 tacaccaaac gagatgaatt agccgaaaat gagttatctg tgccgttaaa attagtgcat
    21301 attaataaat gcccgatttt agcaccggca aaaaccttac tgcctgaaaa tgcagaacgt
    21361 ttaggcatag acagagcttt atgtttggaa aatttgaaaa ctttaaaagc acagaaatcg
    21421 ctgattcggg aaaaagtgat tgaaattttt aatgaagaac gcacgtttga gccttcgacc
    21481 aatgtagaaa ccactttgta tgacggcttt ttctcgcctg cggataaaaa taatatggcg
    21541 attttacgca cgctaccgcc tgaagattta gccaaccacg gcttgaaatt tgaagaccct
    21601 cgagtagagg cgttactatt ccattaccga gcaaggcact atccacaaac actcagtcga
    21661 gccgagcaga tcaaatggca gaaacattgc aaccagcaaa ttgaggcaaa agcagagcag
    21721 ttcgcccaat cgattgacga cttgttccaa caacatcacg acaacccgga aaaggtaaaa
    21781 ttattagaaa atttgaccgc ttacgccgag cagatttcgc aacaacaagc ggtcatttat
    21841 cgtcaaaatg ttgcaaaaga tgaaaaattg ctgagcgagc ttaacagagt agcggaacag
    21901 cccctagata aaaccgaaaa gctcaagatg ttaaaagagt tgattaaatg acaatggata
    21961 atttaccgct agaacagcac ccgtttgacc ctattttacc tgaacaagca acggtggtaa
    22021 tggtcggcac ttttccacct acatcggaaa agcgttgtat ggaatttcat tacccgaatt
    22081 ttcaaaatga tatgtggcga attatgggca atatttttta tgctgaccct aactattttc
    22141 gagtcggtga agaaaaacgc tttgatccga ttcggattga ggagtttttg cgagaaaagg
    22201 gcattgcgct ctgttcttcg gcaaaaacag cgattcgttt aaaaggaaac gcttcagata
    22261 aagatttaaa aattgttgat cctatcgata tggacgaact gttgctcaaa ttacccaaac
    22321 tgaaatggct atttaccaca ggtggtttgg caaccgatac gttgctcggt ttattgccgg
    22381 aaaaacacaa agctccgaaa accaacgaat gggtggtcta tccttattcc gccacaagag
    22441 aactttattt atatcgcctg ccttcgacgt cgagggctta tccactcagc ttagccaaca
    22501 aaaccgaagc ctatcgccaa tttttcagga ctgccggctt aatttaagga taaataacat
    22561 gaaatatgat ttacacgccc acagtaccgc ctctgatggc gtgctttctc ccactgaatt
    22621 ggtacaacgt gcggtagagc aaggggtaac aatgctagct ttaactgatc acgatacgat
    22681 ttcgggcatt caagaagcga aaaattttgc ccaaaatcaa ccgcttgagc ttatctctgg
    22741 ggtggaaatt tcgattttat ggcaagaaaa aagtattcat cttgcggcgt tgaatattga
    22801 tgaaaacaac gaggagctgg tgaagttttt ggataaccaa gcccaattaa gggaagagag
    22861 agcggtcgaa attggtgaaa aattagcaaa agtcgggatt gcaaatgcct atgagggggc
    22921 aaaagcgttg gcaacagggg aagtaactcg ggcacattat gggcgttttt tgtatgaaca
    22981 aggctatgtc cgcaatattg aacacgcctt taagcgttat ctgggaatgg ggaaacctgc
    23041 ctatgtgaaa ccgagatgga gtagtttaga agacgcggtg aatgttaccc acacctcagg
    23101 cggtgtgatt tcgattgctc acccgctacg ctataaaatg actgctcgtt g
//
LOCUS       DANKBV010000029        22921 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.029, whole genome shotgun sequence.
ACCESSION   DANKBV010000029 DANKBV010000000
VERSION     DANKBV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22921
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.029"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..327
                     /locus_tag="PWY43_000860"
     CDS             <1..327
                     /locus_tag="PWY43_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135758.1"
                     /translation="ILDLMFMNEVNLDDLISKLNPSRNGAVIILHETRTPELLECYKS
                     ILSVKLKGEQLDIDLLNNNFKSDFFFNSLFLAIIKRFDKKAFEGNRYIESILLHYKKY
                     LTQETK"
     gene            324..917
                     /gene="hisH"
                     /locus_tag="PWY43_000861"
     CDS             324..917
                     /gene="hisH"
                     /locus_tag="PWY43_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDL2135759.1"
                     /translation="MTNLTIIDTGCANLSSVKFAFDRLGIRAEISRDLNKIQSADKLL
                     LPGVGTAAAAMAILHDRRLIDTIRNATQPMLGICLGMQLMTEFSHEGDVATLNLMSGK
                     TERLPECGLPLPHMGWNKVQYQADHPLFHDIEQGNHFYFVHSYGVLPNENTIATCDYG
                     VPFSAAIASQNFYGVQFHPERSGEAGAKLLRNFVENV"
     gene            1016..1861
                     /locus_tag="PWY43_000862"
     CDS             1016..1861
                     /locus_tag="PWY43_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HDL2135760.1"
                     /translation="MNEIKLFENSQIRSVWDSEKEEWFFSVVDIIEVLTQSSNPRRYW
                     SDLKRKIKDEEGGIELYEKIVQLKLKAPDGKMRETDATDLQGIFRIIQSVPSPKAEPL
                     KMWLAEVGKERIDEIIDPELTIDRALATYLKKGYSREWINQRLQAIQVRKELTDTWQD
                     HGVQAGREFAILTNEITQAWSGMTTRQYKDFKGLKKESLRDNMTTTELVLNMLAEAAT
                     KDIAQISHPQGLEENKTVAKRGGNVAKVARESLEAETGKPVITQQNAIDFAAVIEQVA
                     KQVKK"
     gene            2031..2780
                     /gene="hisA"
                     /locus_tag="PWY43_000863"
     CDS             2031..2780
                     /gene="hisA"
                     /locus_tag="PWY43_000863"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDL2135761.1"
                     /translation="MQKSIIIPALDLIDGQVVRLHQGDYAKQTTYADDPIAQFADYLA
                     QGAEQLHLVDLTGAKNPAQRQTALIGKIIRETHCKIQVGGGIRTEQDVADLLAVGANR
                     VVIGSTAVKERETVKGWFKKYGAEKFVLALDVNIDAIGQKIIAISGWQEASGVSLEEL
                     IEDYQTVGLQHVLCTDISRDGTLAGSNVDLYREICAKYPDIQFQSSGGIGSLADIAAL
                     KGTGVAGVIVGRALLEGKFNVAEAIECWQNG"
     gene            2762..3535
                     /gene="hisF"
                     /locus_tag="PWY43_000864"
     CDS             2762..3535
                     /gene="hisF"
                     /locus_tag="PWY43_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDL2135762.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGA
                     DELVFYDITASSDGRTVDKSWVERVAQVIDIPFCVAGGIKTIADAEQIFAFGADKISI
                     NSPALANPELITELAKRFGVQAVVVGIDSWFEAETGKYWVNQYTGDEKRTRQTEWQLL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLVQLKKVREVCQVPLIASGGAGEMVHFRD
                     AFIEANVDGALAASVFHKQIINIGELKEYLAKEGVEVRR"
     gene            3532..3918
                     /locus_tag="PWY43_000865"
     CDS             3532..3918
                     /locus_tag="PWY43_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2135763.1"
                     /translation="MIKITALDHLVLTVADMQKTIDFYTTILGMQEIAFGDNRKALLF
                     GTQKINLHQKGAEILPNAQNADCGTADLCLLTVTPLQEVIYVLQRHQVEILEGGIVPR
                     TGAVGKIESVYCRDPDGNLIEISRYV"
     gene            3945..4583
                     /locus_tag="PWY43_000866"
     CDS             3945..4583
                     /locus_tag="PWY43_000866"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDL2135764.1"
                     /translation="MQNKIDWQKVDGLLPVIVQNAQTCEVLMLGYMNQQALEKTLAEK
                     RVTFYSRTKQRLWTKGETSGNFLNVVDMSLDCDNDTLLILANPIGETCHTGAESCFHQ
                     FEQEQPDWIFFSKLERMIAERKNADPESSYTAKLYAKGTPKIVQKVGEEAVESVIAAM
                     KNDREELVGEVADLMYHLTVLLHNADLSWAEINAKLKERHKGIGLHPEGSNK"
     gene            complement(4657..5550)
                     /gene="hslO"
                     /locus_tag="PWY43_000867"
     CDS             complement(4657..5550)
                     /gene="hslO"
                     /locus_tag="PWY43_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL2135765.1"
                     /translation="MNYTKDNDKLYRYLFKDRAVRGEWVRLNNTFTETLNTHHYPKAV
                     QNLLGEMLVATSLLTATMKFNGNITVQIQGDGPLKLAVVNGNDQQQLRALARVQADIA
                     DTASLAEMIGNGVLVISIIPTDGERYQGVIALDKPTIRECLEDYFARSEQLQTHLVIR
                     TGEFEGKAVAGGMLLQIMPDGTGSRDDFEHLATLTETVKDEELFGLEAEELLYRLYHE
                     EQVEVYPPQDTEFKCGCSRERSGSALLLVPAEEIEEMLEEKQGVIDMQCECCGTQYFF
                     DKAAIEGFKEETEKLSKLGLN"
     gene            complement(5795..6211)
                     /gene="hslR"
                     /locus_tag="PWY43_000868"
     CDS             complement(5795..6211)
                     /gene="hslR"
                     /locus_tag="PWY43_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDL2135766.1"
                     /translation="MKNKHEKEQNDAEVRLDKWLWAARFYKTRSIAKAMIEGGKVHYN
                     GQRAKVSKAVEIGAIIKLRQGNEEKEVEVLALSDQRRGAPEAQLLYSETEKSIKHREA
                     MVFARKANALSMPHPDRRPNKKERRDLIKFKEQEFS"
     gene            6299..6841
                     /gene="nudE"
                     /locus_tag="PWY43_000869"
     CDS             6299..6841
                     /gene="nudE"
                     /locus_tag="PWY43_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDL2135767.1"
                     /translation="MIKQLPEILNIKTVAKSRIFEVQAVDLRFSNGEERTYERLTPNR
                     RSSVMVIPIQNNELIFVKEYAVGSERYELGFPKGIVDAGEEPIESANRELQEEIGFGA
                     KKLDFLRSLYSSPSHMYGLMHLFLAQDLYPSKLEGDEPEPLEIVRYPLDKIDQLLADP
                     DFAEARNLSALFMLREYLKK"
     gene            6855..7673
                     /gene="cysQ"
                     /locus_tag="PWY43_000870"
     CDS             6855..7673
                     /gene="cysQ"
                     /locus_tag="PWY43_000870"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDL2135768.1"
                     /translation="MQTLTQPLLENIINIAKAAGEQLKQLYARSVAIKIKADCTPVTE
                     ADLIISQFLTERLKQLTPDIPVLSEEFCDIPFAERQQWQEYWIIDPIDGTQQFINRTG
                     QFSVVIALVQKREGRNQPVLGVIHAPILGKTYFSMEGSGSFLQENGEIRPLVKPKRAE
                     NALLIVTVGSDLHAEQAKNAISAPQIECVKYGSGSLKAGLVAEGKADCYIRFGDTGEW
                     DTAVAEVLLSEMGGKIFDFNFSPLTYNQRESLINPYFVMVADGSIDWDKIFLFK"
     gene            7748..9235
                     /locus_tag="PWY43_000871"
     CDS             7748..9235
                     /locus_tag="PWY43_000871"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL2135769.1"
                     /translation="MNAENSCIVIFGASGDLTFRKLIPALYNLYKIGRLGEHFSVLGV
                     SRSELTDESFRQKMRDALVKFEKASGEELDKFCEHLYYQAVNTSDAVDYAKLLPRLDE
                     LHDKYQTAGNTLYYLSTPPSLYGVIPECLAAHGLTTEEFGWKRIIVEKPFGYDIETAK
                     KLDVQIHKCFEEHQIYRIDHYLGKETVQNLLVLRFSNGLFEPLWNRNFIDYVEITGAE
                     SIGVEDRGGYYDGSGAMRDMFQNHLLQVLAMVAMEPPAIINANSMRDEVAKVLYCLHP
                     LTEEDVKHNVVLGQYARGTVDGKEVPAYLEEKGVPADSNTETFMAVKCEIDNWRWAGV
                     PFYVRTGKRLPTRVTEIVIHFKTTPHPVFSQNAPENKLIIRVQPDEGISMRFGLKKPG
                     AGFEAKEVSMDFRYSDLSSSSSLLTAYERLLLDALKGDATLFARTDAVHACWKFVQPI
                     LGYKANRGRVYEYESGTWGPTEADKLIARHGKVWRKPSGTMKKKV"
     gene            9285..9983
                     /gene="pgl"
                     /locus_tag="PWY43_000872"
     CDS             9285..9983
                     /gene="pgl"
                     /locus_tag="PWY43_000872"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDL2135770.1"
                     /translation="MNKVIFPTAQAAVEQIAKEFIEYSHQNRPVHISLSGGSTPKLLF
                     KTLAQAPFNTEIQWQNLHFWWGDDRMVAPTDPESNYGEVQKLLFDHIDIPSENIHRIR
                     GEENVEQELARFSAELTACVPNLAFDWIILGMGTDGHTASLFPHQTDFNDQNVAVIAK
                     HPETGQIRISKTASLIEKAKRITYLVTGASKAEILKEIQTTPAENLPYPAAKIQAENG
                     VTEWYLDSEAANLL"
     gene            10159..11046
                     /locus_tag="PWY43_000873"
                     /pseudo
     CDS             10159..11046
                     /locus_tag="PWY43_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            11056..11745
                     /locus_tag="PWY43_000874"
     CDS             11056..11745
                     /locus_tag="PWY43_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDL2135771.1"
                     /translation="MKTLGILGGMSAESTVSYYLDINRSVNKVLGGNSSAKILISSVN
                     FEEIVQCQKQGNWQAAAEILSEQARLLEQAGAEGILLATNTMHKIADQISSHISVPFL
                     HILDCVAEAISAQGLQKVALLGTKFTMSDNFYKDGLLERGITSIVPDEETQQEIHRII
                     FEELCLGKITESAKAFYLQTIQQLSEQGAEGVILGCTEIGLLINQNDNPLPFFDTAAL
                     HSQMAVEFILG"
     gene            11828..13282
                     /gene="gnd"
                     /locus_tag="PWY43_000875"
     CDS             11828..13282
                     /gene="gnd"
                     /locus_tag="PWY43_000875"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HDL2135772.1"
                     /translation="MSQKGDIGVIGLAVMGQNLILNMNDNGFKVVAYNRTTSKVDEFL
                     EGAAKGTNIIGAYSLEDLAAKLEKPRKVMLMVRAGEVVDQFIEALLPHLEEGDIIIDG
                     GNSNYPDTNRRVKALAEKGIRFIGSGVSGGEEGARHGPSIMPGGDESAWQYVKPIFQA
                     ISAKTDKGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFLKDGLGLSYDEMH
                     EIFKEWKNTELDSYLVDITTDILAYKDEDGEPLVEKILDTAGQKGTGKWTGINALDFG
                     IPLTLITESVFARCVSAFKDQRVAASRLFNKEIGKVEGDKKVWVEAVRRALLASKIIS
                     YAQGFMLIREASEQFGWDINYGNTALLWREGCIIRSRFLGNIRDAYEANPYLVFLGSD
                     PYFKDILENCLADWRKVVAKSVEIGLPVPCMASAITFLDGYTSARLPANLLQAQRDYF
                     GAHTYERTDKARGEFFHTNWTGRGGNTASTTYDV"
     gene            13417..14010
                     /locus_tag="PWY43_000876"
     CDS             13417..14010
                     /locus_tag="PWY43_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL2135773.1"
                     /translation="MLTDQDIFNRYQTKKSEFEKQADISLIGHSLFDMWGDMENGTPN
                     LAGKTVANLGLSGTSTRQYLDVITRQERIQHVGDNVFIFLGVNDIVKEPDYSPKQVLE
                     WIGEIVDSLRKINPNANFFLLEATPVNNITTTNNAAIKELNAYFEANIPSDLTYVKTW
                     EYFADKEGNLDLTLCHDGLHFTQAGYDRLEAILTKHL"
     gene            complement(14081..16945)
                     /locus_tag="PWY43_000877"
     CDS             complement(14081..16945)
                     /locus_tag="PWY43_000877"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2135774.1"
                     /translation="MTKQFTMEDRFNPSAVEQALYQHWESQGYFKPSEDINAPSYCIA
                     IPPPNVTGSLHMGHAFQQTLMDTLIRFNRMEGNNTLWQTGTDHAGIATQMVVERKIAA
                     EEGKTRHDYGREAFINKIWDWKAYSGGTISQQMRRLGNSIDWDRERFTMDEGLSNAVK
                     EVFVRLHEEGLIYRGKRLVNWDPKLHTAISDLEVENKESKGSLWHFRYPLANGAKTAD
                     GLDYLVVATTRPETMLGDTAVAVHPEDERYQALIGKSVILPLANREIPIIADEYVDRE
                     FGTGVVKITPAHDFNDYEVGKRHGLPMVNVMTLNADIRAEAEIIGTDGKPLSDYTAPI
                     PADYQSLERFAARKKIVADFEALGLLDQIKPHDLKVPYGDRGGVPIEPMLTDQWYVSV
                     KPLAEVATKAVEDGEIQFVPKQYENLYFSWMRDIQDWCISRQLWWGHRIPAWYDEQGN
                     VYVARDEAEVRAKHNLPADLPLKQDEDVLDTWFSSGLWTFSTLGWPEQTKELKMFHPT
                     DVLITGFDIIFFWVARMIMFTMHFIKDENGKPQVPFKTVYVTGLIRDEQGQKMSKSKG
                     NVLDPIDMIDGISLDDLLEKRTGNMMQPQLAEKIAKATRKEFAEGIVAHGTDALRFTL
                     TALASNGRDINWDMKRLEGYRNFCNKLWNASRYVLTNDKLDLSEGDVEFSLADRWIES
                     QFNRTVETFRTALSQYRFDLVANAIYEFTWDQFCDWYLELTKPIFFKGTDVQRRGASR
                     TLVNVLEKLLRLIHPVMPFITEEIWQKVKGFVGIEADTIMLQKFPQFDPLAIDETAES
                     QINFIKEVIVAVRNIRAESNIAPSKGLDLIARNFSADEVSILNANEVLLKSMAKLDSV
                     KVLENGENAPLSVAKLVANGEILIPMAGFINKEAELARLTKEMDKLKGEVARIEGKLS
                     NEAFVAKAPEQVIAKEREKMQEYLSGLEKLQVQYQEIEAL"
     gene            complement(17062..17760)
                     /locus_tag="PWY43_000878"
     CDS             complement(17062..17760)
                     /locus_tag="PWY43_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135775.1"
                     /translation="MKQNRKKSKKVIGTHKKENGLKQFIKNNKIPIILISAIWSIFTT
                     DILERIANMPIIWKNFEENYLYNKKLDGLWSTNTNYIIDYKLLNIPEEQPNVIIEFMV
                     NKDDNSILGEMTSTALCELIPLTWSFRLESPKPTLLNLIFDRELKLSYLVSGGYTELS
                     TFKIKEINSNLGTLTLTTQEDKTGIFPPTIKLINFSSNIEDDIKEIEQSCLNSRKQLW
                     EKRRKMQEEQKIPN"
     gene            complement(18005..18598)
                     /locus_tag="PWY43_000879"
     CDS             complement(18005..18598)
                     /locus_tag="PWY43_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135776.1"
                     /translation="MHNNLAIFYCGTNGAGKSTLRSFNQDKVEIVIDSDNIAMQINPL
                     NPRLADIEAGRKAIELFQFAVKNQVAFSMESTLSGKSILKRMENAKAGYFKTRLNYIG
                     VDSYKINIARVKARVKAGGHFIDEETIRKRYHISRENLIEAIFINDETLIYDNSETKP
                     NLILQIADKKVIKISDKIPNWCNQLCDELVELGFKTT"
     gene            complement(18588..18758)
                     /locus_tag="PWY43_000880"
     CDS             complement(18588..18758)
                     /locus_tag="PWY43_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135777.1"
                     /translation="MTSREELLKKQRELDILFTAWFEEKKKHEVLTYRRENGDLIQHY
                     PDGTEKVIEYAQ"
     gene            complement(18760..19506)
                     /locus_tag="PWY43_000881"
     CDS             complement(18760..19506)
                     /locus_tag="PWY43_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL2135778.1"
                     /translation="MNKLAKIEQLKTALSQRRPLSESEVQRLREEFIIESSYNSNAIE
                     GNTITLRETVLILKEGMTIAEKPIWEHLDIIGFKDAFNYIYELVANKEPLSERAIKDI
                     HSLVLMGNAESRGVYRKIPVRILGAENEPPPPYLIAEEMQLLIEKYQQKLAALHPIEA
                     ISWLHLAFESIHPFIDGNGRTGRLLINFELLKQGYLPIDIKFTNRAQYYQCFDDFHKN
                     QQDISSLVELISQYELAELERYLAILKQRT"
     gene            complement(19503..19946)
                     /locus_tag="PWY43_000882"
     CDS             complement(19503..19946)
                     /locus_tag="PWY43_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2135779.1"
                     /translation="MLKNLSWHAKRFDELSTQELFEIYHARTAVFVVEQNCPYQEVDN
                     KDLQAVHFFAKNAKNLTAYCRLIPANDGVHIGRVLVVKENRGSGLVRELVQKAMDYCG
                     EHFPAQPIHAQAQSYLQGFYESFGFKAVSEVYLEDGIAHLDMVLE"
     gene            complement(19940..20377)
                     /locus_tag="PWY43_000883"
     CDS             complement(19940..20377)
                     /locus_tag="PWY43_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDL2135780.1"
                     /translation="MKQVQFYLLSQADAAELSAAEMQACELAAQAWRLSKRVLIACET
                     EQQALNIDEALWARDPDSFVPHNLSGEITTYPTPIEISWKGKRNAQRRDLLISLQTQL
                     PEFIGSFTQIIDFVPVEETAKAQARDRYKQYRQLGWQLETIQC"
     gene            20616..21059
                     /gene="lysM"
                     /locus_tag="PWY43_000884"
     CDS             20616..21059
                     /gene="lysM"
                     /locus_tag="PWY43_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL2135781.1"
                     /translation="MGLFDFVGDIGRKLFNKEEDASKAVTEHLAEDNPGVENVNVTVE
                     NGVAKISGIASTAAAVEKAVLMAGNVAGITEVNIDEVTLPDDVQLASDDELYVIQKGD
                     TLWEIATKAYGNGAKYKAIVEANKEVIKDENKIFPGQKIRIPKGL"
     gene            complement(21113..21964)
                     /locus_tag="PWY43_000885"
     CDS             complement(21113..21964)
                     /locus_tag="PWY43_000885"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2135782.1"
                     /translation="MKQYLDLCNRIVSEGKWVANERTGKHCLTVINADLTYDVEKGEF
                     PLVTTRRSYWKAAIAELLGYIRGYSNAADFRQLGTKSWDANANENAQWLANPYRKGED
                     DMGLVYGAVGRNFPKPDGGSVDLLRQIVDDLKKGIDNRGEIYTFYHPGAFHMGCLRPC
                     LHSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIV
                     NAHIYEDQLELMRDVQLKREPLNAPKLIINPDIKSLEDLETWVSLDDFKVEGYEYHPA
                     IQYPFSV"
     gene            22051..22608
                     /locus_tag="PWY43_000886"
     CDS             22051..22608
                     /locus_tag="PWY43_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135783.1"
                     /translation="MDMKGIMKSILGFSTAVFLAACSSNKSGWTRLNDNDVDQKSYAI
                     AYGATAQTYADRVNESYDINSFMNGVNDWYTKKVTLPIEQIRAITINRMLDHNVYAYY
                     SGVIYAADLQANFNHLDPNCWGLVQPASMTQGIHDAMLDLQKNKVRGDEYIKNGVEQI
                     LHLCVKTIAEDENKSQSKGKTKKHK"
     gene            22817..22911
                     /locus_tag="PWY43_000887"
     tRNA            22817..22911
                     /locus_tag="PWY43_000887"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22852..22854,aa:Ser,seq:gct)
BASE COUNT         6579 a         4624 c         5074 g         6644 t
ORIGIN      
        1 attttagatc tgatgtttat gaatgaagta aatttagatg acttaatttc caagttgaat
       61 ccttctagga atggagctgt aattatattg catgaaacaa gaactccaga gttattggaa
      121 tgttataaat ctattttaag tgtaaaacta aaaggtgagc aactagatat agatttacta
      181 aataataatt ttaaatctga ttttttcttt aatagtttat ttttggcaat tatcaaaaga
      241 ttcgataaga aagcttttga aggaaacaga tatattgagt caattttact tcactataag
      301 aaatacttaa ctcaggaaac taaatgacca acctcaccat catcgacaca ggctgtgcca
      361 acctgtcttc ggtcaaattt gcttttgacc gtcttggcat tcgggctgag atctcgcgag
      421 atctcaacaa aatccaatct gccgataaat tgctactccc cggtgtggga acagcagcgg
      481 cggcgatggc gatcttgcac gatcgccgac ttatcgacac cattcgcaac gccacccagc
      541 cgatgcttgg catctgctta ggaatgcagt taatgacgga attttcccac gaaggcgatg
      601 tggcaacact gaacttaatg agtggcaaaa ccgaacgctt gcctgaatgc ggtttgccgt
      661 tgccgcatat gggctggaac aaggtgcaat accaagccga ccatccgcta tttcacgaca
      721 ttgagcaagg caatcatttc tattttgtgc atagctacgg tgtgctaccg aatgagaaca
      781 ccatcgccac ctgcgactac ggcgtgccgt tttccgctgc cattgccagc caaaacttct
      841 acggcgtaca gttccacccc gagcgttcgg gcgaggctgg ggcgaagttg ctcaggaatt
      901 ttgtggagaa tgtctgattt tgtgatctcg atcgaaaagt ttgaaaatag ggcttgattt
      961 ctcgctttat ttcgacaaaa ttactgttaa tttaaccagt gaaagaaggt gagaaatgaa
     1021 cgaaattaaa ctatttgaaa acagccaaat ccgctcggtg tgggatagcg aaaaagaaga
     1081 gtggtttttc tcggtggtgg acattatcga ggtgctgacg caaagtagca atccacgccg
     1141 ttattggagt gatttaaagc ggaaaatcaa ggacgaagag ggcggaattg aactgtacga
     1201 aaaaatcgta cagttgaaat taaaagcccc agacggaaaa atgcgggaaa ccgatgccac
     1261 ggatttacaa ggcattttcc gtattattca gtctgtgcca agcccgaaag ccgagccgtt
     1321 gaaaatgtgg ctggcagagg taggcaaaga gcggattgat gagattatcg atcctgaatt
     1381 gaccattgac cgagctttgg ctacttactt gaaaaaggga tattcccgtg agtggattaa
     1441 ccaacgcttg caagccattc aggtgcgaaa agaactgacg gatacttggc aagatcacgg
     1501 tgtgcaagcg ggcagagaat ttgcgattct caccaatgaa atcacacagg cgtggagcgg
     1561 tatgactact cgccaatata aggatttcaa agggctgaaa aaggaaagtc tgcgagacaa
     1621 tatgacgacc accgagttgg tgctgaatat gcttgctgag gctgccacca aagacatcgc
     1681 tcaaatcagc cacccacagg gcttggaaga aaataaaacc gttgccaaac ggggcggaaa
     1741 tgttgcaaaa gtggctcggg aaagtttgga ggcagagacc ggcaaaccgg tgattacgca
     1801 acaaaatgcg attgatttcg ctgcggtgat tgaacaagtg gcgaaacagg tgaagaaata
     1861 agtgcggtcg gttttttgca agtttttgga agaatttgac cgcttgtacc ccatacgagc
     1921 cgtatggggc tagataaacc aagctactcc gtagcttaga gaggcagtcg cagcgcgact
     1981 gggattattc agcctagcac ggctcgtgct aggcacacag gaacaataat atgcaaaaat
     2041 ccatcattat ccccgccctc gaccttatcg atgggcaagt agttcgcctg catcagggcg
     2101 attatgcgaa acagacgact tatgccgatg acccgattgc ccaatttgcc gactacttgg
     2161 cacaaggggc ggagcagtta catttggtcg atcttacagg ggcgaaaaat ccggctcaaa
     2221 gacagaccgc acttatcggc aaaattattc gtgaaaccca ctgcaaaatt caagtgggcg
     2281 gcggtatccg caccgagcag gatgtggcgg atttacttgc tgttggagca aaccgtgtgg
     2341 tgattggttc gactgcggtg aaagagcgtg aaacggttaa aggctggttc aaaaagtacg
     2401 gtgcggagaa atttgtgctg gcactggatg tgaacattga tgcaatcggt caaaaaatca
     2461 ttgcgattag cggctggcaa gaggcaagtg gtgtgtcgct cgaggagttg attgaagatt
     2521 atcaaaccgt gggcttgcaa cacgttttat gcaccgatat ttcacgagac ggcacactgg
     2581 caggttcaaa tgtggatctc tatcgagaaa tctgtgccaa atacccagat attcaatttc
     2641 agtcctcagg cggtatcggc tcacttgcag atattgctgc tttaaaaggc acgggcgtgg
     2701 ctggcgtaat tgtcggtcgt gcgttgttgg aaggtaaatt taatgtagcg gaggcaatcg
     2761 aatgttggca aaacggataa ttccttgttt agacgtgcgt gacgggcaag ttgtgaaagg
     2821 cgtgcagttc cgcaaccacg aaatcatcgg cgacattgtg ccactcgccc agcgttatgc
     2881 ccaagagggg gcggatgagt tggtgtttta cgatattacc gcctcaagtg acggcagaac
     2941 ggtggataaa agctgggtgg aacgtgtggc acaggtgatt gatattccgt tctgtgtggc
     3001 aggcggaatc aaaaccattg ccgatgcgga gcagattttc gctttcggag cggacaaaat
     3061 ctccatcaat tccccagccc ttgccaaccc cgagctgatt accgaactgg caaagcgctt
     3121 tggcgtgcag gcggtggtgg ttggtattga tagctggttt gaggcagaaa ccggcaaata
     3181 ttgggtcaat caatataccg gcgatgaaaa acgcacccgc caaaccgaat ggcaattgtt
     3241 ggattgggtg gaggaagtgc aaaagcgagg agcaggcgaa atcgtgctga atatgatgaa
     3301 ccaagacggc gtacgcaacg gctacgattt ggtgcaactg aaaaaagtgc gtgaagtttg
     3361 ccaagtccca ctcatcgcct caggcggtgc aggcgaaatg gtgcatttcc gtgatgcttt
     3421 tattgaggca aatgtggatg gtgcgttggc agcgagtgtg tttcataaac aaattatcaa
     3481 tattggggag ttgaaggaat atttggcgaa ggaaggggtt gaggtgcgta gatgattaag
     3541 attactgcct tagatcatct ggttttaacc gttgccgata tgcaaaaaac cattgatttt
     3601 tatacgacca ttttgggtat gcaagaaatc gcctttggcg ataatcgcaa agcgcttttg
     3661 tttggcacac agaaaatcaa cctccaccaa aaaggtgcag aaatcctacc caatgcccaa
     3721 aatgccgatt gtggcacggc agatttatgc ctcttaacgg taactccact tcaggaggtt
     3781 atctatgtgt tacagcgaca tcaggtggag attttggaag gtgggattgt gcctcgtacg
     3841 ggggcagtgg gaaaaattga gtcggtttat tgccgtgacc ctgatggtaa tttgattgaa
     3901 ataagccgat atgtctaaac aagcggtcaa aaaaggaaga aaaaatgcaa aataaaatag
     3961 actggcaaaa agtggacggc ttactgcccg ttatcgtcca aaacgcccaa acctgcgaag
     4021 tactgatgtt aggatatatg aaccaacaag ccctcgaaaa aacgctggct gaaaagcgag
     4081 tgacttttta ctcccgtact aaacaacggc tgtggacgaa aggcgaaacc tcgggcaatt
     4141 tcctaaacgt ggtggatatg agtttggatt gtgataacga caccctgtta atcctcgcta
     4201 accctatcgg cgaaacctgc catacggggg cggagagctg ttttcatcag tttgaacaag
     4261 aacaaccaga ttggatcttt ttcagcaaat tagaacgaat gatagccgaa cgtaaaaatg
     4321 ctgatccgga aagttcttat accgctaaac tttacgccaa aggcacgcca aaaatcgtcc
     4381 aaaaagtagg ggaagaggca gtagaaagcg tgattgcggc aatgaaaaat gatcgggaag
     4441 agttggtcgg tgaagtggct gatctgatgt atcacttaac ggtgttattg cacaatgccg
     4501 atctctcgtg ggcagagatc aatgctaaac taaaagaacg ccacaaaggg attgggttac
     4561 acccagaagg ctcaaataag taaaatgcaa gcggtcaaat ttcaccgttt ttttacaaat
     4621 tgcaaaaaag acaagaaact tgaccgcttg tttagcttaa tttaacccta acttacttaa
     4681 tttttcagtt tcttccttaa agccttcaat cgccgctttg tcaaagaaat attgtgtccc
     4741 gcagcattca cattgcatat cgatcacgcc ttgtttttcc tccaacattt cttcgatttc
     4801 ttctgccggc accagtaata atgcggagcc tgaacgctct cttgagcagc cacatttaaa
     4861 ttcagtatct tgtggtgggt aaacttctac ttgctcttcg tggtagaggc gatagagcaa
     4921 ttcttctgcc tctaaaccaa ataactcttc atcttttacg gtttcagtga gcgtggctaa
     4981 atgctcaaaa tcatctcttg agcctgtgcc gtccggcata atttgtagca acataccgcc
     5041 tgccactgct ttgccttcaa attcgcctgt gcggataacg aggtgggttt gtaattgctc
     5101 ggagcgggcg aagtaatctt ctaaacattc acgaatggtt ggtttatcga gagcaatcac
     5161 cccttgataa cgttcaccat cggttgggat aatagaaatc actaatacac cattgccgat
     5221 catttccgct aacgaggctg tgtctgcaat atctgcctga acacgggcta aagcacggag
     5281 ttgctgttgg tcgttaccgt tcacaaccgc caattttaac gggccgtcac cttgaatctg
     5341 cacagtaatg ttgccgttaa atttcattgt tgcagtcagc aaactggttg cgactaacat
     5401 ttctccgagt aaattttgta ctgctttagg gtagtgatgg gtgtttaagg tctcggtaaa
     5461 ggtgttgttt aaacgcaccc attcgccccg taccgctcgg tctttgaata aatagcggta
     5521 gagtttgtca ttgtctttgg tgtaattcat tttgattcct cgttgcaagc ggtcgattat
     5581 tactcacaaa attatttcta attttgttcg ctcctacgga gccgttggca aagccaacgt
     5641 tcaaaatgca atgcattttg tggctaaaaa tgtgcaaatc gaacgctgta ggggcgaatc
     5701 aatattcgcc cgaaataatt atataggggc gaaaaatttt tcgcccctac attaggatat
     5761 tcaatatagg gattattttt ctgattgcaa gggcttagct aaattcttgc tctttgaatt
     5821 tgattaaatc ccgccgttct tttttgtttg gacggcgatc agggtgtggc attgaaagag
     5881 cattcgcttt gcgggcaaat accattgcct cacggtgttt aatgcttttt tcggtttcac
     5941 tgtagagcag ttgggcttcc ggagcccctc ggcgttggtc actgagggct aacacttcca
     6001 cctctttttc ttcattgcct tggcgaagtt tgatgatggc accgatctcc accgctttgc
     6061 taactttagc tctttgccca ttataatgga ctttaccccc ttcaatcatt gctttggcaa
     6121 tcgaacgggt tttataaaaa cgagccgccc aaagccattt atctagccga acttcggcgt
     6181 cgttttgttc tttttcgtgt ttatttttca ttttctgcct taaaatcaaa acaatagtcg
     6241 aaatattaga gcgaaagtga gatttttcaa ctaaaataac tcaaaaaggt gagataaaat
     6301 gataaaacaa cttcctgaaa ttttgaacat taaaacagta gcgaaatctc gcatttttga
     6361 ggtgcaagcg gtcgatttac gcttttctaa tggcgaagag cgaacttacg aacgacttac
     6421 gccgaatcgc cgttcttcgg taatggtgat tccgattcaa aacaacgagc tgatttttgt
     6481 gaaagagtat gcggtcggct cggagcgtta cgagctgggt tttccgaaag gcattgtgga
     6541 tgcaggtgaa gagccgattg agagtgcgaa ccgtgaatta caagaagaaa ttggttttgg
     6601 ggcgaaaaaa ttagattttc tgcggtcgct ttacagttcg ccaagccata tgtacggctt
     6661 aatgcacctg tttttggcac aagatctgta cccatcaaaa ttggaaggcg atgagcctga
     6721 accgctggaa attgtgcgtt atccgttgga taaaattgat caattattgg cagatcctga
     6781 ttttgccgaa gcacggaatt tgtccgctct atttatgcta cgagaatact taaaaaaata
     6841 ggcaaggagc tattatgcaa acgctaactc aacccctact cgagaatatt atcaatattg
     6901 ccaaagcagc cggtgagcag ttaaagcagc tttatgcccg ctcggtggca attaaaatca
     6961 aagccgattg cacaccggtg acggaagcgg atctgattat cagccaattt ttaactgaac
     7021 gcttgaagca actcacacca gatattccgg tattatcgga agagttttgc gatattccgt
     7081 ttgccgaacg ccaacaatgg caagaatatt ggattatcga cccgattgac ggcacacaac
     7141 agtttattaa ccgcaccggt caattttcag tggtgattgc gttagtgcaa aaacgtgagg
     7201 gtagaaatca gccggtgctg ggcgttattc atgctccgat attaggaaaa acctatttta
     7261 gtatggaagg tagcggtagc tttttgcaag aaaatggaga aattcgaccg cttgtcaaac
     7321 cgaaacgtgc ggagaatgcc cttttgattg ttaccgtagg cagcgattta catgcagaac
     7381 aagctaaaaa tgccatttca gccccacaaa ttgagtgtgt aaaatatggt tcgggcagtc
     7441 taaaagccgg tttagtggca gaagggaaag ccgattgtta tatccgtttt ggtgacaccg
     7501 gcgaatggga tactgctgtg gcagaagtgc tgttaagtga aatgggcggc aaaattttcg
     7561 actttaattt ttcgccgctg acttataatc agcgagaaag tttgattaat ccttattttg
     7621 tgatggtggc tgacggctcg atagattggg ataaaatatt tttattcaaa taggtagttc
     7681 cgattagcaa tttttatgat aatcttgcac ccaacaaaat tttaacaatt agcaatagga
     7741 taaataaatg aatgctgaaa atagctgtat cgtcattttt ggtgcttccg gtgacttaac
     7801 ttttcgtaaa ttaattcctg ctctttataa cttatataag attggcagat tgggcgagca
     7861 tttttccgtc ctaggtgttt ctcgttcaga attaaccgac gaatctttcc gccaaaaaat
     7921 gcgtgatgcg ttagtcaaat ttgaaaaagc aagcggtgaa gaattagata aattttgcga
     7981 acatctttat taccaagctg taaatacctc tgatgcagtg gattatgcca agttgctgcc
     8041 tcgtcttgat gagctacacg ataaatatca aacagccggt aacacgcttt attacctctc
     8101 cacgccacca agtttatatg gcgtgattcc ggaatgttta gccgctcacg gcttaactac
     8161 cgaagagttt ggctggaaac ggattattgt tgaaaaacca ttcggctacg atatcgaaac
     8221 cgccaaaaaa ttagacgtac aaatccacaa atgctttgaa gagcatcaaa tctaccgtat
     8281 cgaccactat ttaggtaaag aaacggtaca aaacttactc gttttacgtt tttctaacgg
     8341 cttatttgaa ccgctttgga accgtaattt tattgattat gtggaaatta ccggggcaga
     8401 atccatcggc gttgaagatc gtggcggtta ctatgatggc tcgggggcaa tgcgtgatat
     8461 gttccaaaac cacttattgc aagtattagc catggttgca atggagccac cggcaattat
     8521 taatgctaat tcaatgcgtg atgaagtcgc aaaagtgcta tattgcttgc atccgctaac
     8581 tgaagaagac gttaaacaca atgtggtgtt aggtcagtat gctcgtggca cggtagacgg
     8641 caaagaagtg ccggcttact tggaagaaaa aggcgtgccg gctgattcca acactgaaac
     8701 ctttatggcg gtaaaatgtg aaatcgataa ctggcgttgg gcaggtgtgc cgttctatgt
     8761 gcgaaccggt aaacgcttac caacccgtgt gacggaaatc gtaattcatt ttaaaactac
     8821 accacacccg gtatttagcc aaaatgctcc ggaaaataaa ttgattattc gtgtgcaacc
     8881 ggacgaaggc atttcaatgc gtttcggctt gaaaaaaccg ggagcaggct ttgaagcgaa
     8941 agaagtctca atggatttcc gctattctga tttaagttca tcatcaagct tactcaccgc
     9001 ttatgaacgc ttactcttag acgcacttaa aggtgatgca accttatttg cccgtactga
     9061 tgctgtacac gcttgttgga aatttgtgca accgatttta ggctacaaag ctaatcgtgg
     9121 gcgtgtgtat gaatatgaat ccggcacttg ggggccaacc gaggcagata aattaatcgc
     9181 cagacacggc aaagtatggc gaaaaccgtc cggtacaatg aagaagaaag tgtaaatttc
     9241 agttcccctc ttttctaaag aggggtgatc taacgaggca aattatgaat aaagttattt
     9301 tccccaccgc ccaagctgcg gttgaacaaa ttgctaaaga gtttattgaa tacagccatc
     9361 aaaaccgccc ggtgcatatt tccctttccg gtggttcaac ccccaaatta ttattcaaaa
     9421 ccttagctca agcaccgttt aataccgaaa ttcaatggca aaacctgcat ttctggtggg
     9481 gggatgatcg aatggttgcc ccaaccgatc ccgaaagtaa ctacggcgaa gtgcagaaat
     9541 tactgttcga tcacatcgac attccaagcg aaaatattca ccgcattcgt ggtgaagaga
     9601 atgtagaaca agaacttgca agattttcgg cagaattgac cgcttgtgtg ccgaacttgg
     9661 cgtttgattg gattatttta ggaatgggaa cagacgggca taccgcctca ctgttcccac
     9721 atcaaacaga ttttaatgat cagaacgttg ccgtgatcgc caaacaccca gaaacagggc
     9781 agatccgcat ttccaaaacg gcatctttaa ttgaaaaagc caaacggatc acttacttgg
     9841 tcacaggagc aagcaaagcg gaaattttaa aagaaatcca aaccactccg gcagaaaatt
     9901 taccttatcc tgccgccaaa attcaggcgg aaaatggggt aacggaatgg tatttggata
     9961 gtgaggcggc taacttactc taggtcacaa tcagtgacct ttacaagcgg ttaaatttta
    10021 gaaaaatgtt gcaaagtatt tttgtgatat tcatcgaaaa agcagaaaaa tctgctcgtg
    10081 gtataggcca aaaattggca tagtctttct tattttgaga tcacaaaagt gatcttcact
    10141 gaagaataag gaagaaagat gaatccaatt caattaattg aaccaaacat taactctaaa
    10201 atttttacta ttcgtggcga gcaagtaatg ctagatgttc atttggcaga gctttatcag
    10261 gtagatactc gaacctttaa ccaagcagta aaacggaatg aagagcgatt tcctgacata
    10321 tttcgttttc aattaacaga aaacgaatgg ctatctttaa gatcacaatt tgtgatctta
    10381 aacgaaaata gtgggcgtgg ccaacatagg aaatatcttc cttatgtctt taccgagcaa
    10441 ggcattgcaa tgctttcggc agtattgcgt agtgaagttg cagtgcaagt tagcattaag
    10501 attatgcaag cctttgtgca aatgcgtcat cttttgcaaa caaatagtgg tttggtacaa
    10561 cgcttagaaa gtgtagagcg taaacagttg gagatgtctg ggtagttaga gcaggtgttt
    10621 caatctcttg aaggcaataa gcctaaaatg caaggggttt tctttgaagg tgaaaccttt
    10681 aacgcttatc agtttgttac ggaattagtg agaggtgcaa aatcatcgct gattttaatt
    10741 gataactatg tagatgatac cgttttaact ttatttacga aacgatcgca taacgtatca
    10801 gttacgattt atacgaaaca agtatctaag caacttgcat tagatttgga aaaatataac
    10861 agccaatatg ctcacattaa tgtgaaaata tttgataaat ctcacgaccg atttttgatt
    10921 attgatgaaa aagagcttta tcatatcggt gcatcactta aagatttagg taagaaatgg
    10981 ttcgcttttt ctaaaatgga tttatcaatg agccgcattt tagaaaagtt agcggaaaat
    11041 caataaggta tattaatgaa aacactcggc attttaggtg gaatgagtgc ggaaagcacg
    11101 gtttcttatt atttagatat taatcgctcg gtgaataaag tattaggtgg aaatagcagt
    11161 gccaagattt taatttcgag tgtcaatttt gaagagattg tacaatgcca aaagcaggga
    11221 aattggcaag cagccgctga aattttatct gaacaagcta gattgctaga acaagccgga
    11281 gcagaaggca ttttattggc aaccaacaca atgcacaaaa ttgccgatca aattagtagc
    11341 catatctctg tgccgttttt gcatattttg gactgtgtgg ctgaggcgat ttcagctcaa
    11401 ggtctgcaga aagtagcact ccttggcaca aaatttacta tgagtgataa tttttacaaa
    11461 gacggtttat tggagcgtgg tatcacatca atagtaccag atgaggaaac gcaacaagaa
    11521 attcatcgca ttattttcga ggaactctgt ttaggcaaga ttactgaaag tgccaaagcg
    11581 ttttatcttc agactattca acaacttagt gaacaaggtg cagaaggtgt gattttaggt
    11641 tgtaccgaaa tcgggttgct gattaaccaa aacgacaatc cgttaccgtt tttcgataca
    11701 gcagcattac acagccaaat ggcggtggag tttattttag gttaataaat gttacaagcg
    11761 gtcaaatttg caaaaaattt taccaattcg accgcttgca tagaatcaaa aacaatagga
    11821 gcaaacaatg tcacaaaaag gcgatatcgg cgtgattggg ctcgccgtaa tgggtcaaaa
    11881 tttaattttg aatatgaatg acaacggctt taaagtggtg gcgtacaacc gcactacttc
    11941 aaaagtggat gagttcttag aaggtgcggc gaaaggcacg aacattatcg gtgcttactc
    12001 gttagaagat ttagcggcga agttggaaaa accgcgtaaa gtgatgctaa tggtgcgtgc
    12061 cggtgaggtg gtggatcagt ttatcgaggc tcttctgcca catttagaag aaggcgacat
    12121 catcattgat ggcggtaact caaactatcc ggacaccaac cgccgtgtga aagcgttagc
    12181 ggaaaaaggc attcgcttta tcggctcagg tgtatcgggc ggcgaagagg gtgctcgtca
    12241 cggaccgtca attatgccgg gcggtgatga atctgcgtgg caatatgtga agccgatttt
    12301 ccaagcaatt tcagccaaaa ccgacaaagg tgagccttgt tgtgactggg ttggtcgtga
    12361 tggagcaggg cattttgtga aaatggttca caacggtatc gaatatggcg atatgcagtt
    12421 aatttgtgaa gcctaccaat tcttaaaaga tggtttaggt ttaagctatg atgaaatgca
    12481 cgaaatcttt aaagagtgga aaaacaccga attagacagc tatttggtgg atattaccac
    12541 tgatattctt gcctacaaag acgaagacgg cgagccattg gtagagaaaa ttctcgacac
    12601 cgcaggtcaa aaaggaacgg gtaaatggac tggtattaac gcccttgatt tcggcattcc
    12661 attaacactg attaccgagt cggtattcgc ccgttgcgta tctgcgttta aagatcaacg
    12721 tgttgcggct tcaagattgt ttaacaaaga aattggtaaa gttgaaggcg acaaaaaagt
    12781 atgggtagaa gcggtacgcc gtgcgttact tgcttccaaa atcatctctt atgcacaagg
    12841 ctttatgttg attcgtgagg cttctgagca attcggttgg gacatcaact acggcaatac
    12901 cgccctatta tggcgtgaag gttgtattat ccgcagccgt ttcttaggca acattcgtga
    12961 tgcgtatgaa gccaacccat atttagtgtt cttaggctca gacccatact tcaaagatat
    13021 tttggaaaac tgcttggcag attggcgtaa agtagtggca aaatcggtgg aaatcggttt
    13081 acctgtacct tgtatggcgt cagccattac cttcttagat ggctacacct cggcacgttt
    13141 accggcaaac ttactccaag cacaacgtga ctactttggt gctcatactt atgagcgtac
    13201 agacaaagct cgcggtgagt tcttccacac caactggaca ggacgtggcg gtaataccgc
    13261 atcgaccact tatgatgtat aattaatctt tttgagccac ttaattcgat attaagtggc
    13321 ttttttattg gatttttctt caaaatatct tggagtgaat gtttacaagt ttgtataata
    13381 atggagcgat ttttcattta tatttaagaa gaacctatgc ttaccgatca agatattttt
    13441 aatcgctacc aaacaaaaaa atcagaattt gaaaaacagg cagatatttc ccttattggg
    13501 cattccctgt ttgatatgtg gggcgatatg gaaaacggca cgccgaattt agcaggaaaa
    13561 acagttgcca atttgggctt atcaggcacc agcacccgcc aatatttgga tgtgattacc
    13621 cgacaagagc gaattcaaca cgttggcgat aatgtattta tttttctagg cgttaatgac
    13681 attgtaaaag agccggatta ctcgccaaaa caagtgttag agtggattgg cgaaattgtg
    13741 gattctcttc gcaaaattaa tccaaatgcc aacttttttc tattggaagc cacaccggtt
    13801 aataatatta ccaccaccaa taatgctgca ataaaagagc tcaatgcgta ttttgaggca
    13861 aatattccat ctgatttgac ttatgtaaaa acgtgggaat attttgctga caaagaaggg
    13921 aatttagatc taactttatg ccacgatgga ttacacttca cccaagcagg ctatgaccgc
    13981 ctagaggcga ttttaaccaa acatttataa tgaaaagctt caataagcaa gcggtctgaa
    14041 ttagcaaaaa atttccaaat cctgaccgct tgttagttaa ttacagtgct tcaatctctt
    14101 gatactgcac ctgcaatttc tctaatccac tcaggtactc ttgcattttt tcacgctctt
    14161 tggcgatgac ttgctctggg gctttggcga caaaggcttc gtttgaaagt ttgccttcaa
    14221 tgcgagccac ttcaccttta agtttatcca tctctttggt gaggcgagcc agctcagctt
    14281 ctttgttgat aaagcctgcc attgggatca agatctcacc atttgcgacc agtttcgcta
    14341 ccgatagcgg tgcattttca ccattttcca gcactttcac gctatcaagt tttgccatcg
    14401 atttgagcaa cacttcgtta gcgttgagaa tagagacttc gtccgcagag aaattacgag
    14461 cgattaaatc taaacctttg cttggtgcga tgttgctttc cgcacgaatg ttacgcaccg
    14521 ccacaatcac ttctttgata aagttgattt gactttcggc ggtttcatca atggcaagcg
    14581 ggtcaaattg tgggaatttt tgcaacataa tggtatcggc ttcaatacct acaaagcctt
    14641 tcactttctg ccagatttct tcggtgatga acggcattac tgggtggatt aaacgcagga
    14701 gtttttccaa tacattcacc agcgtgcgac ttgcgccacg gcgttgtaca tctgtgcctt
    14761 tgaagaaaat aggtttggtc agctctaaat accagtcgca gaattgatcc caagtaaatt
    14821 cgtagatcgc atttgccact aagtcgaaac ggtattggct gagtgcggtg cggaaagttt
    14881 ccaccgtgcg gttgaattgg ctttcaatcc aacgatcggc aaggctaaat tccacgtcgc
    14941 cttcgcttaa atccaacttg tcattggtca gcacataacg gctcgcattc cacagtttgt
    15001 tgcagaagtt gcggtagcct tctaaacgtt tcatatccca gttgatgtct cgaccgttgc
    15061 tggcaagggc ggtgagggtg aaacgcagag cgtctgtgcc gtgtgccaca atcccttcgg
    15121 caaattcttt gcgggttgcc ttagcgattt tttctgccag ttgtggctgc atcatattac
    15181 ctgtacgttt ttcgagcaaa tcgtcgagac tgatgccgtc aatcatatca atcgggtcta
    15241 acacgttccc tttcgatttc gacattttct gcccttgctc gtcacggatc aagcctgtta
    15301 catacacggt tttgaacggc acttgcggtt tgccgttttc atctttgatg aagtgcatcg
    15361 tgaacataat catacgagcc acccagaaga agatgatgtc aaaaccggta atcagcacat
    15421 cggtcgggtg gaacattttc agttctttgg tttgttctgg ccagcctaac gttgagaacg
    15481 tccatagacc agacgagaac caagtatcca gtacgtcttc atcttgtttt aatggtaagt
    15541 ccgcaggtaa gttgtgtttc gcacgcactt ccgcttcatc tcgtgctaca taaacattgc
    15601 cctgctcgtc ataccacgct ggaatgcggt gtccccacca taattggcga gagatacacc
    15661 agtcttgaat atcacgcatc caagagaaat acaggttttc gtactgtttc ggcacgaatt
    15721 ggatttcgcc gtcttccacc gctttggtcg cgacttccgc caaaggtttg acagacacat
    15781 accattggtc ggtcagcatc ggctcaatcg gcacgccacc acggtcgcca tacggcactt
    15841 ttaagtcgtg tggtttgatt tgatctaaca gcccaagagc ttcaaaatct gccacaattt
    15901 tcttacgcgc ggcaaaacgc tctaagcttt gatagtcggc aggaattggg gcggtgtagt
    15961 cggaaagcgg tttaccgtct gtgccgataa tttcggcctc tgcacgaata tcggcattta
    16021 aggtcatcac attcaccatc ggcaagccgt ggcgtttgcc cacttcgtag tcgttgaaat
    16081 cgtgggcagg ggtgattttt accacgcccg taccgaactc acgatccaca tactcatcgg
    16141 caataatcgg gatttcacgg ttggcaagcg gtaggattac gcttttgcca atcagagctt
    16201 gataacgctc atcttcaggg tgaaccgcca ccgcagtatc gcccaacatc gtttcaggac
    16261 gggtcgttgc caccactaag taatccaagc cgtctgcggt ttttgcacca ttagccagcg
    16321 gatagcggaa atgccacagc gagcctttgc tttctttgtt ttccacttcc aagtcagaaa
    16381 tcgcagtatg cagtttcgga tcccagttta cgaggcgttt gccacggtaa atcaagcctt
    16441 cttcgtgcaa gcgaacgaac acctctttta ccgcattgga taagccttcg tccatcgtaa
    16501 aacgctcacg atcccagtca atcgagttgc ctaagcgacg catttgctgg ctgatcgtgc
    16561 cgcccgaata ggctttccaa tcccagattt tgttgataaa ggcttcacgc ccataatcgt
    16621 ggcgggtttt accttcttcc gctgcgattt tacgctccac caccatctga gtggcaatcc
    16681 ctgcgtggtc agttcctgtt tgccaaaggg tattgttgcc ctccatacgg ttaaaacgaa
    16741 tcagcgtatc catcaaggtt tgctggaaag cgtgccccat atgcaaactc cccgtcacat
    16801 tcggcggcgg aatagcgata cagtagctcg gtgcattaat atcttcactc ggcttaaaat
    16861 agccttgcga ttcccagtgt tggtagagcg cttgctctac agcggaaggg ttaaaacggt
    16921 cttccattgt gaattgtttt gtcattggtt ttgttttctc ttgtttaata attttttcac
    16981 tagcttaatc ttataaaaaa gatctttgcc gtaaatgctt ttataggtaa ttagttattt
    17041 aaattaaggt caactagaag attaattggg aattttttgt tcctcttgca tttttcttcg
    17101 tttttcccat aattgctttc ggctatttaa acagctttgc tcaatctctt taatatcatc
    17161 ttctatattg gatgaaaaat tgattaattt gattgtcgga ggaaatatac cggttttatc
    17221 ttcttgcgta gttaatgtta gtgttcctag attactgtta atttctttaa ttttaaatgt
    17281 tgatagctcg gtatatcccc cactaacaag ataactcaat ttcaactctc tatcgaaaat
    17341 aagatttaat aatgttggct tagggctttc caatctaaaa ctccaagtta gaggtattaa
    17401 ctcacacaga gctgttgaag tcatttcccc taaaatcgaa ttgtcatctt tattaaccat
    17461 aaattcaatg ataacatttg gttgctcttc aggtatgttc aataatttat agtcgataat
    17521 gtaatttgta tttgtagacc ataaaccatc cagtttttta ttatataggt aattctcctc
    17581 gaaatttttc caaataatag gcatgtttgc gatgcgttct aaaatatcag ttgtgaagat
    17641 actccatatg gcagatataa gaataatagg aattttgtta ttttttatga actgttttaa
    17701 gccattctct tttttatgag taccaataac ttttttagac tttttcctat tctgtttcat
    17761 ctttgtgttt ttgttccaat atccccgatg gtgatcgtct tcgacgagag cttatttatt
    17821 taagaaccta tcagagatag gcataagcgg tctgtttcga gaagtttttt gcaaagtttt
    17881 tctttaatcc gacagcttgt cacccacaca caagccgtgt ggggttacgc atagctgagc
    17941 cactttgtgg ctctaagtta cagctccaaa ggagctggga ttgtcgagcc tagcacggct
    18001 cgtgctaggt ggttttaaac cctaattcca ctaattcatc acaaagctga ttacaccaat
    18061 tcggtatttt gtctgaaatt tttatcactt tcttatcagc aatctgcaaa atcaaattcg
    18121 gcttggtttc tgaattatca taaatcagtg tttcatcatt gataaaaatc gcttcaatta
    18181 aattttcacg gctaatgtga tagcgttttc taatggtttc ttcatcaata aaatgtccgc
    18241 cggctttcac tcttgctttt acccgtgcga tgttaatttt atagctatcg acaccaatat
    18301 aatttaatcg agttttgaaa tatcctgctt tggcattttc cattcgtttt agaatagatt
    18361 tacccgagag ggttgattcc attgaaaatg caacctgatt ttttactgca aactgaaata
    18421 gttcgattgc ttttctgccc gcctcaatat cggccaatct tggattgagt gggttaatct
    18481 gcatcgcaat attatcggaa tcaatcacaa tctcgacttt atcttgattg aaagaacgta
    18541 aagtagattt gccggctccg tttgtgccac agtaaaaaat tgctaggtta ttgtgcatat
    18601 tcaatcactt tttccgtacc gtccggataa tgctgaatta aatccccatt ttctcggcga
    18661 tacgtcagca cttcgtgctt tttcttttct tcaaaccatg ctgtaaagag aatatccagt
    18721 tcacgttgtt ttttcagtaa ttcttcacga cttgtcattt tatgtcctct gtttcagaat
    18781 cgccaaatac ctttccaact cagctaattc atactgacta atcaattcaa ccaaactact
    18841 aatatcttgt tgatttttat ggaaatcatc aaaacattgg taatattggg ctcgattggt
    18901 aaatttaata tcaatcggta aatagccctg ttttaataat tcaaaattaa tcagtaatcg
    18961 ccctgtgcgc ccgtttccat caataaaagg gtgaatgctt tcaaaggcta aatgcagcca
    19021 actgattgcc tctatgggat gaagtgcggc cagtttttgt tgatattttt caatcaataa
    19081 ttgcatttct tcggcaatta aataaggggg gggtggttca ttttctgccc ctaaaattcg
    19141 tactggaatt ttgcgataaa cccctcggct ttccgcattg cccattaata ccagagaatg
    19201 aatatcttta atcgctcgtt cgcttaatgg ttctttattg gcgactaatt cataaatata
    19261 gttgaatgca tccttaaaac cgataatatc taaatgctcc caaatcggtt tttcggcaat
    19321 ggtcattccc tcttttaaaa tgagcaccgt ttcacgcaaa gtaatggtat tgccttcaat
    19381 tgcattggaa ttatagctgc tttcaataat aaactcttcc cgcagtcgtt gaacttcgct
    19441 ttcagataat ggacggcgtt gtgaaagtgc tgtttttaat tgttcgattt tagctaattt
    19501 attcattcta acaccatatc caaatgcgca atcccatctt ccaaatacac ttctgaaacc
    19561 gctttaaacc caaaactttc ataaaaccct tgcaggtaac tttgggcttg ggcgtgaatg
    19621 ggttgagcag gaaaatgttc gccacaataa tccatcgctt tttgtaccaa ttcccttact
    19681 aagcctgagc cacgattttc tttcaccacc aacactctac ctatatgcac accatcattg
    19741 gctggaatta aacggcagta agcggtcaga ttttttgcat tttttgcaaa gaaatgcacc
    19801 gcttgcaggt ctttattatc gacttcttgg taagggcagt tttgctcaac cacaaacacc
    19861 gctgtacgtg cgtggtaaat ctcaaacaac tcttgggtgg atagttcatc aaagcgtttt
    19921 gcgtgccaag agaggttttt tagcattgta tcgtttccag ttgccaacca agttggcggt
    19981 attgtttata gcggtcacga gcctgtgctt tggcggtttc ttccaccggc acgaaatcaa
    20041 taatctgggt aaaactgccg ataaattcgg ggagttgggt ttggagtgaa atcaacaaat
    20101 cccgacgttg agcattgcgt ttacctttcc acgaaatttc gataggcgtt gggtaagtgg
    20161 tgatttctcc cgataaattg tgcggcacga acgaatccgg atctcttgcc caaagtgctt
    20221 cgtcaatatt taacgcttgt tgctcggttt cgcatgcaat cagtacccgt ttacttaatc
    20281 gccacgcttg agccgcaagt tcgcaggctt gcatttcagc agcggaaagc tcggctgcat
    20341 cggcttggct taatagataa aattgtactt gtttcacaga atgttcccag aaattcaatc
    20401 agatatttaa acttaatatt ctagctgaaa atgagtaaaa tataaaatct aagcgtttgt
    20461 ttttgcaaaa aattgagaaa aaatgaccac ttgtgttgcg ttgccttgag gaactaatta
    20521 gcttttagac gttctaagag aacaatgcac tatttcaata gttggaaatt gtgtagaata
    20581 cccacttcat ttagctattt tataaggaac ttactatggg cttatttgat tttgttggcg
    20641 acattggtcg taaattattt aataaagaag aagatgcttc aaaagcggtt acagagcatt
    20701 tggcggaaga caatccgggc gttgaaaatg taaacgtaac ggttgaaaac ggggtagcga
    20761 aaatttcagg cattgcttca accgcagcag ctgttgagaa agcggtattg atggcgggta
    20821 atgtggctgg tatcactgag gtaaatattg atgaagtgac cttgcctgat gacgtgcaac
    20881 ttgcaagtga tgatgagctt tatgttatcc aaaaagggga tacgttgtgg gaaatcgcca
    20941 caaaagctta tggtaacggt gcaaaatata aagcgattgt agaagcaaat aaagaagtta
    21001 ttaaagacga aaataaaatc ttcccagggc aaaaaattcg tatcccgaaa ggtttataat
    21061 caaattgaaa gagctaagct ggtcgaaagt ttagctcttt ttgtaggtgg tgttagactg
    21121 aaaatggata ttgaatggca gggtggtatt cataaccttc aactttgaaa tcatctaagc
    21181 tcacccaagt ttctaaatct tctaaggatt ttatatcagg attaatgatt aatttaggtg
    21241 cattgagtgg ttctcgctta agttgaacat cacgcattaa ttctagttga tcttcataaa
    21301 tatgcgcatt gacaatttta tggaacgctt gtcctgcctt atgccctgta atttgagcca
    21361 ttaaagctaa aaaggtatag cattgaatca tattccaatt taacccaagc ggtacatctg
    21421 ccgaacgctg agtactattc aggtaaagcg tgccatcaag tagagaaaaa tgatggctgt
    21481 gtaaacaagg acgtaagcag cccatatgga aagcgccggg gtggtagaag gtataaattt
    21541 caccacggtt atcaattcct tttttcaaat cgtccacaat ttgtcgcaat aaatcgactg
    21601 agccaccgtc tggtttcggg aaattacgcc ctactgcgcc gtaaaccaag cccatatcat
    21661 cttccccttt tcgataggga ttagcaagcc attgtgcatt ttcatttgca ttagcgtccc
    21721 acgattttgt gcctaattga cggaaatcag cagcattact atagcctcga atatagccta
    21781 aaagttcggc aatcgcagct ttccaataac tacggcgagt ggtaactaaa ggaaattctc
    21841 ctttttctac gtcataagtt aagtcagcat taatgactgt gaggcagtgt ttgcctgtac
    21901 gttcatttgc aacccatttg ccttcgctaa cgatgcggtt acataagtct aaatactgtt
    21961 tcattattta cctcataaaa aattttaggt aattttgcca tttattcggt aaaatagcta
    22021 aaactttatg acacaacaca ataaggagtt atggatatga aaggtataat gaaatccatt
    22081 ctgggttttt ccaccgctgt ttttttagca gcttgttcat cgaataaaag cggttggaca
    22141 cgtttaaatg ataatgatgt tgatcagaaa tcttatgcta ttgcttatgg tgcaacggct
    22201 cagacttatg cggatagagt aaatgaaagc tatgatatca attcctttat gaatggggta
    22261 aatgattggt acactaaaaa ggttacattg ccaattgagc aaattagagc aataacgatt
    22321 aatcgaatgt tagaccataa tgtgtatgct tattatagtg gtgtgattta tgccgcagat
    22381 ttgcaggcaa atttcaatca tttagatcct aattgttggg ggcttgttca accagcgagt
    22441 atgacccagg gcattcacga tgcgatgtta gatttacaga agaacaaagt tcgtggagat
    22501 gagtatatca aaaatggggt tgagcaaata ttgcaccttt gtgtaaaaac gattgctgaa
    22561 gatgaaaata aatcgcaatc taaaggtaaa accaaaaagc ataaataagt taagctatga
    22621 caagcggtaa aatttttaaa aaattttacc gcttttttat aaccgtatga aatgtttaat
    22681 atttattcta tgaaacttaa aaaaacagcg ttttgtttat tcaataggca aacactcggg
    22741 atttttcaat tttcaggaaa aagaatttga catcttttta aattctagta atatggcgcc
    22801 cacagacaca gtgagtggtg agatggccga gcaggctgaa ggcgctcccc tgctaaggga
    22861 gtatagggtt aaaaagctct atcgagggtt cgaatccctc tctcaccgcc atttacttta
    22921 t
//
LOCUS       DANKBV010000030        21374 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.030, whole genome shotgun sequence.
ACCESSION   DANKBV010000030 DANKBV010000000
VERSION     DANKBV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21374
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.030"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(52..1587)
                     /gene="der"
                     /locus_tag="PWY43_000888"
     CDS             complement(52..1587)
                     /gene="der"
                     /locus_tag="PWY43_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL2135784.1"
                     /translation="MTPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGH
                     ANIAGYDFIVIDTGGIDGTEEGVEEKMAEQSLLAIEEADVVLFLVDARAGLLPADVGI
                     AQYLRQREKTTVVVANKTDGIDADSHCAEFYQLGLGEVEQIAAAQGRGVTQLIEQVLA
                     PLGEQLNAEQFNQEQAGENAKNVANEETDEWDTEFDFENEEDAALLDEALAEEAEESI
                     EDKNIKIAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGQQYTIID
                     TAGVRKRGKVNLAVEKFSVIKTLQAIQDANVVLLTIDAREGISDQDLSLLGFILNAGR
                     SLVIVVNKWDGLSQDIKDQVKSELDRRLDFIDFARVHFISALHGSGVGNLFDSVKEAY
                     ACATQKTSTSMLTRILRMAADEYQPPLVNGRRVKLKYAHPGGYNPPIIVIHGNQVEKL
                     ADSYKRYLSNYFRKSLKIIGSPIRILFQEGNNPFAGKKNKLTPSQLRKRKRLMKFIKK
                     SKK"
     gene            complement(1772..3079)
                     /locus_tag="PWY43_000889"
     CDS             complement(1772..3079)
                     /locus_tag="PWY43_000889"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDL2135785.1"
                     /translation="MTILALGINHKTASVGLREKVAFVDDKRKLALEQIQTSGLAESV
                     VILSTCNRTELYFHQPNISPREESEENIQWREQCFRWFAQIHQLDESELRECLYFKQN
                     LEAANHLMKVASGLDSLILGEPQILGQVKQAYQYSEDFYQHQQKAMFTKLSRLFQRTF
                     ATAKRVRSETEIGSQAVSVAYAACGLAHQIFDNFGKLRFLLVGAGETIELVARYLIQH
                     GAKNIMLANRTHIRAEMLAEKLNAPMQILSLSALQLGLNQADVVISSTGSPDTLISKE
                     MVEIAQKQRQFDPLLLIDIAVPRDIDEKAGDLEGVYAYSVDDLQNIIQRNMAQREEAA
                     KQAAEIVKEESKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLEKALQAISQGQDSQKV
                     LSELSYKLTNQLLHAPTQALQAMAKSGNSTGLQSFSRALRLEE"
     gene            3333..5531
                     /gene="relA"
                     /locus_tag="PWY43_000890"
     CDS             3333..5531
                     /gene="relA"
                     /locus_tag="PWY43_000890"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDL2135786.1"
                     /translation="MVAIRYSHQLEPNNFELSSWCASLNMSPVTFEQIHNAWSYAQEK
                     LDTDYSLMWYGIEMVEVLHGLNMDDDSLVAAMLFPLVKHNVIDLVQLKEDFSNDIKNL
                     VKGVLEMENIRQLSANNVSDLQIDNIRRMLLAMVDDFRCVVIKLAERIAYLRDSNHHS
                     EEDTVLAAKECSHVYAPLANRLGIGQLKWELEDYCFRILHPVSYTQIAKFELGERRVD
                     RERYIANFVADLTACLSPEIKNVQIYGRPKHIYSIWKKMQKKRLRFNQLFDIRAVRVI
                     VPTLEDCYTALGIIHTKYTHLPEHFDDYIANPKPNGYQSLHTVVLGEGDKTIEVQIRT
                     QQMHDDAELGVAAHWKYKEGATAGRSGYEEKIVWLRKLLEWQNDLADSGDMVAEMRSQ
                     IFDDRVYVFTPRGEVIDLPQKATPLDFAYAIHSEIGHRCIGAKVAGRIVPFTYHLQMG
                     DQVEIITQKEPNPSRDWLNPNTGFVNTGKARSKIIAWFKKLDREKNIPIGKEALEAEM
                     EKFGFTLKQIEEYALPRYNLKQFDDLYAGIGGGDIRLHQLMHFLQSKLIKPTAEQEDE
                     AVLKQVNKNTNQPKPSKGYVIVEGVGNLMHSIARCCQPIPGDEIVGYITQGRGISIHR
                     ADCEQLFELQSLNPERVVEADWGEGYTSGLSLTIRVIANDRNGLLRDVSAIMANEKVN
                     VLGVSSRTDVKRSLATIDMEIQLNNVEILNKLLARIAQLDDVIEAKRLSS"
     gene            5650..6840
                     /gene="ispC"
                     /locus_tag="PWY43_000891"
     CDS             5650..6840
                     /gene="ispC"
                     /locus_tag="PWY43_000891"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDL2135787.1"
                     /translation="MKKLVILGSTGSIGKSTLSVVEHNPNQYEVFALVGGKNVELMLE
                     QCRQYQPTFAAMDDEKSAQALKTALATLGSKTEVLAGQAAICELAAHSEVDMVMAAIV
                     GAAGLLPTLSAVKAGKTVLLANKESLVTCGQIFIDEARKSGAKLLPVDSEHNAIFQSL
                     PPEAQQKVGFCPLAELGVSKIILTGSGGPFCTKPLNEFDAITPAQAVAHPNWSMGKKI
                     SVDSATMMNKGLEYIEARWLFNATAEEMDIIIHPQSIIHSMVRYIDGSVIAQMGNPDM
                     CTPIAHTMAYPNRINAGVPPLDFFKLKELTFIEPDFARYPNLKLAIEAFAEGQYATTA
                     MNAANEIAVEAFLNEQIRFTDIVNVNRTVVENIAPIQVSEIADVLHIDKLARELAKQA
                     VLQL"
     gene            complement(6883..8265)
                     /gene="nhaC"
                     /locus_tag="PWY43_000892"
     CDS             complement(6883..8265)
                     /gene="nhaC"
                     /locus_tag="PWY43_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2135788.1"
                     /translation="MNNYLLKLKPMTAVILSLLTVFILGFTMIGLGWVPQISILAVIV
                     MLLLFGLINKIPFKNMQEGMASGLMSGIGAVYLFFFIGLLVSALMMSGAIPTLMYYGL
                     SVISPELFYISAFALSSVVGIALGSSLTTTATIGVAFIGMASAFGANPAIVAGAVVSG
                     AFFGDKTSPLSDTTTIAASVVGIDMFAHIRNMLYTTIPAWILTALFLWFLTEPTDAQN
                     LANIENLKTNLQETGLIHGYSLLPFVTLILMAIKRVNSIYIIVTTVTLSLIITYIHSS
                     PTLEQLGNYFFTSYKPVEGVDLGEVASLVSRGGINSMFFTITIVLLALSLGGLLQILG
                     ILPALLNGISGFLTSTGRVTLVVASTAVGINVLIGEQYLSLLLSGNTFRSTYERLGLE
                     VKNLSRTIEDAGTVINPLVPWGVCGVFISSVLNVPVLDYLPYAFFCYICLILTLIFGF
                     TGVTISKKAN"
     gene            complement(8311..9897)
                     /locus_tag="PWY43_000893"
     CDS             complement(8311..9897)
                     /locus_tag="PWY43_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDL2135789.1"
                     /translation="MIQITQTINIDLLIVGSGIAGLSAAVEAKEKGINTLLISKSTIG
                     SGASYFPLKATLGIQVTGDEADKIHFKEDIENIGKGKINPNVVQAYIEDSPQAIELLH
                     RIGFQPWKRNDNRPACFAKYPRPIYLIKDWRESALRAKQILQEQHIDFYENATLLHIV
                     TDNNQVQGAVFSLTTSGQISYIFCKTKHLILAAGGIAGLYKDNLYPADVIGSLHYVAQ
                     QAGATLVNLAFIQFIPAFVEPKYKVLFGEHTLKYVTQITDSKGNNLFPHLNAEQFAKM
                     VLERSHYAPFSLDFAGVEFDLVMMKHLLENPHEKGIYLKYSPKLYEDTEEFYTVYLNW
                     LKNEVGIDLVQDKIAIKPFAHSCNGGIQINQWGESAVKGLFAIGEISHCIEGANRMGG
                     NSVGGGLVFAKRAVSEIVRSLEQNDLQKNSEISPLAMAEKALNQLANSTANLELTASE
                     VLSTIREKMALYANVYRTRSNLKLLLEELNRLEQEFDPIYHAQYQGIEIYNALKTAQL
                     VVNQMLNEQSLGAHYLDEQN"
     gene            complement(9961..10767)
                     /locus_tag="PWY43_000894"
     CDS             complement(9961..10767)
                     /locus_tag="PWY43_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2135790.1"
                     /translation="MSMKFIKEQPIIELSNIVVQFASRDGTLFNPKKFTAVNDVSLAI
                     HAGETVGLVGQSGCGKSTLASVMIGLQKPTSGTVKFNGLEMKYGSAEARKYFGSQVSV
                     IFQDPATALNPRMRVLDILKDPMDIHNILQPQEREKRVYDLLSRVGLPRSAALVEPTR
                     LSGGQKQRVAIARALALNPKLIVADEPTSALDVSVRAQVLNLLADLKKELNLAMVFIS
                     HDLQTVHQVSDRIVVMNGGKIVEMGDASDVFERPTQEYTRTLIEAAPSLL"
     gene            complement(10782..12752)
                     /locus_tag="PWY43_000895"
     CDS             complement(10782..12752)
                     /locus_tag="PWY43_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDL2135791.1"
                     /translation="MFRQGLAARLASGGARFRALSTSSKIALLFIVFIALVAIFAPLV
                     APYDPLQTLRPVQAPSGDYLFGTDRLGRDIFSRMVWGARTSLFIGLGAVGVAILFGSI
                     LGATAATADKFGNEVIMRFMDILMAFPGIALAAVLLATFGNSVPVIIVTIAVVYTPQL
                     ARVVRANVVSQWEEDYVRAERVIGGSRTYILLKHVVRNTAAPVLVFATVMVADAIVFE
                     ASLSFLGAGVQPPFPSWGNILSEGRNLVLSGFWWATTFAGIMILLTVLALNILAEGLT
                     DALVNPKLKRSTQSKEDVAKPLATDVQEAMSETLALKRYLLKLHEKETTRTDRMQLNS
                     TAKPILQVKNLSIRFPNRYGETPLVDNISFTVHEGETMGLVGESGCGKSITAFSIMGL
                     LPKTAKITGEILFTDRSGKQHNLLESADLNALRGHEISMIYQDALSALNPSMRIKDQM
                     AQLISRGGKQSAETLLQWVKLDPEKTLNRYPHELSGGQRQRVLIAMALAREPKLLIAD
                     EPTTALDVTVQAEVIKLLNELREKLGFAMVFVSHDLALVAQIAHHITVMYAGQVVETA
                     PTTPLLANPTHEYTRGLLGSVLSTELRAERLYQIPGSVPSPFDFAKGDRFASRSLRPD
                     ANPEQHLKLVPTGDHPQHFWASHLEEPQSKEA"
     gene            complement(12754..13707)
                     /locus_tag="PWY43_000896"
     CDS             complement(12754..13707)
                     /locus_tag="PWY43_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2135792.1"
                     /translation="MEIILRLLWRRLMALPVMILGVTALVFIVLQFTPGDPATVALGE
                     SASEAAKEIYREQHGLNDPLLVQYFRFLGNLFVLDFGMTTPPEQPIVDMIAKAFPLTL
                     QLTFIGVFLAAIVSFTLGVTAALYRDTWIDQLIRLISVAAVATPSFWLGILLIQYFSL
                     KLDWLPSGGFIPFSEDPKGYVNSMVLPSLALAVPVCASLIRVVRTTMVEEMDKDYVRT
                     AIGNGVPYSTVIRHNVLRNALITPVTVLGLRVGYLLGGAVVIEQIFDLPGMGKLIFNG
                     IVNHDLNLVQGVVLTIAFTFVLVNIIVDILYLLINPKIRSL"
     gene            complement(13919..15502)
                     /locus_tag="PWY43_000897"
     CDS             complement(13919..15502)
                     /locus_tag="PWY43_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2135793.1"
                     /translation="MKKQFEHNESRRGFMKLIAGVGAGMAFSGTLGTFTPKAFAAPAA
                     GSTIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLVDLHPATRKPYLALAAAEPEK
                     IDDVTYRITLRDGAKFHNGNPVTTEDVVYSFERVLDPAKASLFAQFIPFIDTVKKVDD
                     KVVEFKLKYPFALFKERLTIVKIVPKAVVEAGQAAFDANPVGTGPYKFVSATKDDRIV
                     FEAFADYNGGYPAQVEKMTWFLLSDDAARVTAQESGRVQAIESVPYLDAERLKRKGTV
                     ESVQSFGLLFLMFNCEKAPFNNPKVRQALHYGLDTQKLIDVVFLGNAKAATSYVQDTH
                     PDYVKANSQYDFDKAKAEALLAEAGVTELKFELLATDHSWVKECAPLILESWNSLKGV
                     KVSLKHLQSGALYGTHVDKGAYEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRW
                     SETAEYAEVQKLLDEAAKNPAASKEAWAKAINIIAEQVPLYPIIHRKLPTAWNDKALT
                     GFQPLPTTGMSFIGVGRAK"
     gene            15725..16882
                     /gene="hemW"
                     /locus_tag="PWY43_000898"
     CDS             15725..16882
                     /gene="hemW"
                     /locus_tag="PWY43_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDL2135794.1"
                     /translation="MNLILPPLSLYIHIPWCVQKCPYCDFNSHAQKGLIPEQEYIEHL
                     LADLSQDLTAYRVAIGDRKLYSIFIGGGTPSLFSAEGIAYLLSEVEKRIAFEPNIEIT
                     LEANPGTAEAERFLGYAQGGVTRISMGIQSFEPEKLLKLGRIHDANEAKQAIKFAQNS
                     AKSGLQSFNIDLMHGLPNQSVKQALVDLETGISLNPPHLSWYQLTIEPNTMFYYRKPT
                     LPDDDELWDIFEQGHQLLTNAGYEQYETSAYAKKGYQCRHNLNYWRFGDYLAIGCGAH
                     GKISYPTGEIYRFSKTKHPKGYMRGEYRYSQDLIELDDRPFEFFMNRFRLLEATPKQE
                     FEWFTGLDREIVRPTIDWALNQNYISESSTHWQITQHGKLFLNELLEGFLD"
     gene            17024..17672
                     /locus_tag="PWY43_000899"
                     /pseudo
     CDS             17024..17672
                     /locus_tag="PWY43_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            17759..18226
                     /gene="bcp"
                     /locus_tag="PWY43_000900"
     CDS             17759..18226
                     /gene="bcp"
                     /locus_tag="PWY43_000900"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDL2135795.1"
                     /translation="MNILQAGDKAPQFTLQNQADESVSLSQFAGKKVLVYFYPKALTP
                     GCTTQACGLRDSKSELDELNVVVLGISTDLPKKLAQFVEKKALNFTLLSDPEHQVAEA
                     FGVWGEKKFMGKTYDGIHRISFLIDEQGVVEQVFDKFKTGEHHQMIVDYLRGR"
     gene            18226..18774
                     /gene="gmhB"
                     /locus_tag="PWY43_000901"
     CDS             18226..18774
                     /gene="gmhB"
                     /locus_tag="PWY43_000901"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDL2135796.1"
                     /translation="MGKKTIFLDRDGTINIDHGYVHQIDDFQFIDGVGKALKQLQDKG
                     YLLVLVTNQSGIARGYFSEAQFMQLTEWMDWSLDEDYGVVLDGIYYCPHHPEGKGEYR
                     EDCDCRKPKAGMFTQAIQDLNIDPSQSYMVGDKLEDILAAEAAGVKTKVLVRTGKAVT
                     EEGQAKADLVLDSLVDLVKYIK"
     gene            18779..19618
                     /gene="queF"
                     /locus_tag="PWY43_000902"
     CDS             18779..19618
                     /gene="queF"
                     /locus_tag="PWY43_000902"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDL2135797.1"
                     /translation="MNYNDKSLSALKLGQKTEYKSEYDASLLQPVPRKLNRDGLGITQ
                     EQPFNRGADVWTCYELSWLNQNGLPQVAIADVAIDFKSENLIESKSFKLYLNSFNQTK
                     FSSIEEVEKTIQADLARCASGQVSVKIYQLSHYTQQPIVDFAGDCIDEQDIQIDSYAF
                     STQHLENIAENEPVEEILVSHLLKSNCLITSQPDWGSVQIRYKGKKLNCEKLLRYLVS
                     FREHNEFHEQCVERIFCDLMQFAQPEKLTVYARYTRRGGLDINPFRSNFEEVPENLRM
                     ARQ"
     gene            19810..20943
                     /locus_tag="PWY43_000903"
     CDS             19810..20943
                     /locus_tag="PWY43_000903"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDL2135798.1"
                     /translation="MLIFMMNEYSPNYYIGVMSGTSLDGVDIALLDFAKNPPKMTACD
                     FFPMPEELRADISALLKTGETNLQKLGEIDHRLGLLYAETINAFLAKNQLKAEDIQAI
                     GCHGQTVWHSPDSEYPFTTQIGDMNLVAEQTGITTIADFRRKDMAVGGQGAPLVPAFH
                     QAIFSDPNRLTVVLNIGGISNISVLNPNSPTVGYDVGAGNTLMDSWIEQHQGKRYDKN
                     GEWARTGKLNTELLATLLDESFFAKAPPKSTGRELFNLTWLAKKLENLTACQPQDVQR
                     TLAEFTAQSIANELLRFESSLPRLLLVGGGGARNPLLMERISALLPEWEVTTTTEFGL
                     DIDYVEAAAFAWLAYQRIHNLPSNMPSVTGAKKAVSLGVIYPA"
     gene            complement(20940..>21374)
                     /locus_tag="PWY43_000904"
     CDS             complement(20940..>21374)
                     /locus_tag="PWY43_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2135799.1"
                     /translation="LFIPLGLGSAFYIIFPYIFTNLLDKPELVSVSFFVNNLIAVLLQ
                     SDFSRKLNLTVHKLIYIAPILIILQIMPWFYALQWLSTITAFLYLVIFATITVYANTA
                     LSNMLVKLDNGKNQGLMFGSSKLILAITTLGVMNVLPYIFLI"
BASE COUNT         6358 a         4279 c         4613 g         6124 t
ORIGIN      
        1 tgccattatt agttttagca caagcgtgga cgcttgcgct atcgttagat attatttctt
       61 acttttctta ataaacttca tcaaacgttt acgtttgcgt aattggcttg gtgtcagttt
      121 gtttttctta ccggcaaaag gattattgcc ttcttggaat aaaatccgaa ttggcgagcc
      181 gataattttt aaacttttac ggaaataatt acttaaataa cgtttgtaag aatccgctaa
      241 tttttcaacc tgattgccgt gaatcacaat aattggtggg ttgtagccac ccgggtgagc
      301 atattttaat ttcactcggc gaccattaac caatggtggt tggtattcat cagcagccat
      361 acgcaaaatg cgagtcagca ttgaggtcga ggttttttgc gttgcacaag cgtaggcttc
      421 ttttactgaa tcaaacaaat tccccacgcc tgagccgtgc aatgccgaga taaaatgcac
      481 tctggcaaaa tcaataaaat ctaaacggcg atctaactct gatttcactt gatctttaat
      541 atcttgcgac aagccgtccc atttgtttac cacaatcacc aatgaacgac cagcatttag
      601 aataaagccg agtagtgata aatcctgatc ggaaatacct tctcgggcat caatagttaa
      661 tagcaccacg tttgcatctt gaatggcttg caaggtttta attaccgaga atttctcaac
      721 cgctaaattc actttgccac gtttacgcac accggcggta tcaatgatgg tatattgctg
      781 accgtcacgc tccattggaa tataaataga atcacgggtt gttcctggca tatcataaac
      841 taccacacgc tcttcgccta aaatgcggtt ggtcagtgtt gatttaccaa cattcggacg
      901 accgacaatc gcaattttga tatttttatc ttcaatcgac tcttctgctt cttctgccaa
      961 ggcttcatct aataatgccg catcttcttc gttctcaaag tcgaattcgg tatcccattc
     1021 gtctgtttct tcatttgcaa catttttagc attttcgccc gcttgttctt ggttaaattg
     1081 ctcggcattt agctgttcgc ctaaaggagc taacacttgc tcgataagct gcgtaacccc
     1141 acgcccttgt gctgcagcaa tttgttctac ttcgcctaag cctaattgat agaattcggc
     1201 acagtgagag tcggcatcaa taccatcggt tttattggca accaccaccg tggttttttc
     1261 acgttggcgt aaatattgag caatgccgac atcggcaggt aataatccgg ctcgggcatc
     1321 aaccaagaaa agcaccacat cggcttcttc aatcgccaat aaagattgtt ccgccatttt
     1381 ttcttccacg ccttcctctg taccatcaat accaccggtg tcgattacaa tgaaatcgta
     1441 gccggcaata ttagcgtgac catatttacg gtctcgggtt aagccgggga aatctgctac
     1501 taaggcatct cgagtacgag ttaaacggtt aaataaagtc gatttgccga cattaggtct
     1561 gcccactagg gcaacaacag gagtcatttt gtatcctcta tatccggtta tttgcaaaat
     1621 ggttgcaaaa attggcttat tatagcggaa atttggggaa gttgaaatta gaagaagatc
     1681 gggaacacgg ggagggacgc acggcgtgca tccgcaagcg gttaaaaatg ttcaattttt
     1741 tgcaaatatg cctaaaagaa tgtgctgagc atcattcttc tagccgtaat gctcttgaaa
     1801 aactttgtaa gccggtggag ttgccacttt ttgccattgc ttgtaacgct tgggttgggg
     1861 cgtgtagtaa ctggttggtg agtttgtagc ttagctcact taatactttt tggctgtctt
     1921 gcccttgact gattgcttgt agggctttct ccaacaattc ttggcgggtt tgctccgcat
     1981 cttgacgata gcgtttgatt aaatcgctag actgctgttg ttttaaccat tcaaaaaatg
     2041 ctttgctttc ctctttgacg atttcagcgg cttgtttggc ggcttcttca cgctgagcca
     2101 tattgcgttg gataatattt tgtaaatcat caacactata agcgtaaacg ccctctaagt
     2161 cgccggcttt ttcgtcaata tcacgaggaa cggcaatatc aatcagcaaa agtgggtcaa
     2221 attggcgttg tttttgcgca atttccacca tttctttact gattagggta tcagggctac
     2281 cggtggagct aataactaca tcagcttggt ttaaaccgag ttgcaaagca cttaacgaca
     2341 gaatttgcat tggagcgttt agtttttccg ccaacatttc cgcccgaata tgggtgcggt
     2401 ttgccaacat tatatttttc gccccgtgtt gaattaaata gcgggcgact aattcaatgg
     2461 tttcgcctgc ccctaccagc aaaaagcgta acttgccaaa attatcgaaa atttgatggg
     2521 ctaaaccgca agctgcataa gcaactgaca ccgcttggct gccgatttcg gtttcggaac
     2581 gtactcgttt tgcggtggca aaggtgcgtt ggaacaagcg tgatagcttg gtgaacatcg
     2641 ctttttgctg atgttggtag aaatcttcgc tatattgata ggcttgttta acctgcccta
     2701 aaatttgtgg ttcgcctaaa attagtgaat ctaatccgct tgccactttc attaagtggt
     2761 tagcggcttc taaattttgt ttaaaatata aacattcacg caactcgctt tcatcaagtt
     2821 gatgaatttg ggcaaaccag cggaaacatt gttcacgcca ttggatattt tcctcgctct
     2881 cttcacgggg agaaatatta ggctggtgga agtagagctc ggtgcggttg caggtagaga
     2941 gaatgaccac gctttcagcc aatccgctcg tttggatttg ttcaagagcc agtttacgtt
     3001 tgtcatctac aaacgccact ttctctctta aacccacaga ggcggtttta tggttgattc
     3061 ctaaagctaa aattgtcatt gctacccaca aaaataaaga tcaaaataaa aacgcaagta
     3121 ttttaatcaa gagcgtgaag tcgtgcaaat gattgtgttg ataggtttga tttatcacaa
     3181 ggttgctacc tcttaaggag taaagaggat aagataactt ggattaaatt ttcgattagg
     3241 ggtaaatttt ttctgaattt ccacgtataa taaaactcca ttttaagctg tacaagcggt
     3301 ctaatttcca caaaaattta caaggaggcc ttatggtagc aattcgttat tcccatcaac
     3361 ttgaaccgaa taattttgag ttgtcgagtt ggtgtgcaag cctgaatatg tctcctgtga
     3421 cttttgagca gattcacaat gcgtggagtt atgcacagga aaaactggat accgactata
     3481 gtctgatgtg gtatggcatt gagatggttg aagtattgca cggcttgaat atggacgatg
     3541 acagcttagt tgccgcaatg ctttttcctc tggtaaaaca caatgtgatc gacttggtgc
     3601 aattaaaaga agatttcagt aacgacatca aaaacttagt taaaggcgta ctcgaaatgg
     3661 agaatatccg ccagcttagt gccaataatg tgtcggatct gcaaatcgac aatatccgtc
     3721 gcatgttgtt ggcgatggtg gacgatttcc gctgtgtggt gattaaactt gccgaacgaa
     3781 ttgcctacct gcgtgatagt aaccaccatt ccgaagaaga taccgttctc gccgctaaag
     3841 aatgttctca cgtttatgca ccgcttgcca atcgattggg tatcgggcag ctcaaatggg
     3901 agctggaaga ttactgtttc cgcattttac accctgtctc ttacactcaa attgctaaat
     3961 ttgaattggg cgaacgccgt gtggatcgtg agcgttatat tgcaaatttt gtagcagatc
     4021 tgaccgcttg tttaagtcct gaaatcaaga atgtgcagat ttatggacgc ccgaaacata
     4081 tctacagcat ttggaagaaa atgcagaaaa aacggttgag atttaatcag cttttcgata
     4141 ttcgggcagt gcgggtaata gtgccaacac tagaagattg ctacacggca ttaggcatta
     4201 ttcacaccaa atatacccat ttgccggaac attttgatga ttatattgct aacccgaaac
     4261 ctaacggcta tcaatcatta cacaccgttg tattaggcga gggcgataaa accatcgaag
     4321 tgcaaattcg cacccagcaa atgcacgatg atgccgagct tggggtggcg gctcactgga
     4381 aatacaaaga gggagcaacc gcagggcgtt cgggctacga agaaaaaatt gtctggttgc
     4441 gtaaactcct tgagtggcaa aatgatcttg ccgattcggg cgatatggtg gcggaaatgc
     4501 ggtcacaaat ttttgatgat cgtgtttatg tttttacccc gagaggtgag gtgatcgatt
     4561 taccgcaaaa agctacgccg cttgattttg cttatgcaat tcatagtgaa atcgggcatc
     4621 gttgcattgg agcaaaagtg gcagggcgga ttgtgccatt cacttatcat ctgcaaatgg
     4681 gcgatcaggt tgaaattatc acccaaaaag agccgaaccc aagccgagat tggctcaatc
     4741 cgaataccgg ttttgtgaat acaggcaaag ctcgctcaaa aattattgcg tggtttaaaa
     4801 aattagaccg agagaaaaat attccaatcg gcaaagaggc ccttgaagcc gaaatggaaa
     4861 aattcggttt tacgctcaag caaattgaag aatatgctct gcctcgctat aacttaaaac
     4921 agtttgatga tctctatgcc ggtattggcg gtggcgatat tcgtcttcat cagttaatgc
     4981 acttcttgca atcaaaatta attaaaccga ccgccgaaca agaagacgaa gcggtactta
     5041 aacaggtgaa taaaaacacc aatcagccga agccaagcaa aggctatgtg attgtagagg
     5101 gcgtagggaa cttaatgcat tccattgccc gttgctgcca gccgattccg ggtgatgaaa
     5161 tcgtgggtta catcacacaa ggcagaggga tttcgattca ccgtgcagat tgtgagcaac
     5221 tgtttgaatt acaaagtctc aacccagaac gagttgtgga ggcggattgg ggcgaaggct
     5281 atacctctgg tttaagctta accattcgag tgattgccaa tgaccgcaac ggcttactgc
     5341 gagatgtcag tgcaattatg gcaaatgaaa aagtaaacgt gttaggcgtt tccagccgaa
     5401 ccgatgtgaa acgtagcctt gccaccattg atatggaaat tcagcttaac aacgttgaaa
     5461 ttttgaataa attattggct cgtatcgctc aattagatga tgttatcgaa gcaaaacggc
     5521 tttcgagtta atctacaagc ggttgttttt gctaaacaat ttacaaacaa cgaatgagta
     5581 tccgcataat tacggcgtag gggcgaaaaa tctttcgccc ttatcagtca ttagataaag
     5641 agaaacaaaa tgaaaaaatt agtgatttta ggttcaacag gttctatcgg taaaagcact
     5701 ttatcggtag ttgagcataa cccaaaccaa tatgaggtgt ttgcgttagt tggtggaaag
     5761 aatgttgaat taatgctgga gcaatgtcgt caatatcagc cgacttttgc ggcaatggat
     5821 gatgaaaaat ccgcacaagc attaaaaacg gcattggcaa ctttggggtc taaaactgaa
     5881 gtgttagccg gtcaagcagc tatttgtgag cttgctgcac attccgaagt ggatatggta
     5941 atggctgcta ttgtaggagc agcagggtta ttaccgacac tttctgcggt gaaagcgggt
     6001 aaaaccgtat tgttggcaaa taaagaatcg ctggtaacct gtgggcaaat ttttattgat
     6061 gaggctcgta aatccggtgc gaagctctta ccggtagata gtgaacacaa tgcgattttc
     6121 caatcgcttc caccggaggc acagcaaaaa gtcgggtttt gtccactcgc tgaactgggt
     6181 gtcagtaaaa ttattttaac aggatcgggt ggaccgttct gtactaagcc attaaatgaa
     6241 tttgatgcga ttacaccggc tcaagcagtt gctcacccca attggtctat gggtaagaaa
     6301 atttcagtcg attcagccac aatgatgaac aaaggcttgg agtatattga agcccgctgg
     6361 ttgtttaatg caacagccga ggaaatggat atcatcattc acccacaatc aattattcat
     6421 tcgatggtgc gttatattga tggctcggtg attgctcaaa tgggcaaccc ggatatgtgt
     6481 acgccgattg ctcacacgat ggcatatcca aaccgaatta atgctggtgt gccaccatta
     6541 gatttcttta aattaaaaga gctaaccttt atcgagccgg attttgcccg ttacccgaac
     6601 ttaaaattag cgattgaggc ttttgcagaa ggacagtatg cgacaacagc gatgaatgcc
     6661 gccaatgaaa ttgccgttga ggcgttttta aacgagcaaa tccgctttac ggatattgtg
     6721 aacgtgaata gaacggtggt ggaaaatatt gctccaattc aagtcagtga aattgctgat
     6781 gtattgcata ttgataaact cgccagagag ttagccaaac aagctgttct tcagctctaa
     6841 tgaaaagaaa atgcctacaa atttgtaggc atgtttcaca tattagttag cttttttact
     6901 gattgttacc ccggtaaatc caaaaattaa ggtaaggatt aaacagatat aacagaagaa
     6961 tgcgtatggt agataatcta acaccggtac atttaatacg ctactgataa acacaccgca
     7021 cacgccccaa gggactaatg ggttgattac cgtgcctgca tcttcaatgg tgcgagataa
     7081 gtttttcact tctaggccta aacgctcata agttgagcgg aaagtattac ctgataacaa
     7141 taagctcaaa tattgctcac cgattaagac gttaattccc accgcagtgc tggcaactac
     7201 aagagtaaca cgtcctgtgc tggttaaaaa gccgctaatg ccgttaagta gtgccggtaa
     7261 aatgccgagg atttgtaaaa gaccacctaa acttaacgca agcaatacaa tagtgatggt
     7321 aaagaacata ctattaatgc caccacgaga gactaatgat gccacttcgc ctaaatctac
     7381 gccttcaaca ggtttatagc tggtaaagaa ataattccct agctgttcaa gggttggcga
     7441 actgtggata taggtaataa ttagcgagag tgttactgta gttacaataa tataaattga
     7501 attaacacgc ttaattgcca ttaaaattaa cgtaacaaaa ggtagcaaag agtagccgtg
     7561 tattaatcct gtttcttgca aattggtttt taggttctct atatttgcta aattttgtgc
     7621 atcagtaggt tcggttaaaa accaaaggaa taatgctgtt aaaatccacg cagggatggt
     7681 ggtataaagc atattacgga tatgagcgaa catatcaatg cccactacgg aagcagcaat
     7741 ggtagtagtg tctgaaagtg gagaggtttt atcgccaaaa aacgcccctg aaaccaccgc
     7801 acctgctaca atcgcagggt tcgcaccgaa agcagaagcc ataccgataa aagcaacacc
     7861 gatggtggcg gttgtggtta aactactgcc taacgcaata cctactacag aacttaatgc
     7921 gaatgcagaa atgtaaaata attcaggtga aatcacactt aagccataat acattaaggt
     7981 tggaatcgca ccagacatca tcaaagctga aaccaataat ccaataaaaa agaataggta
     8041 aaccgcacca ataccggaca tcagcccgct tgccataccc tcttgcatat ttttaaacgg
     8101 aattttattg attaagccga aaagtagcag cattacaatc acggctagaa ttgaaatttg
     8161 aggcacccag ccaagcccaa tcattgtaaa acctaagata aagaccgtca aaagcgaaag
     8221 tatgaccgct gtcattggtt ttaatttaag taggtagtta ttcatttata ctcctaattt
     8281 attattttta aataagttgg ctattataga ctaattttgt tcatctaagt agtgcgcgcc
     8341 taaactctgc tcatttagca tttgattgac cactaattga gcggttttta acgcattgta
     8401 aatttcaatg ccttgatatt gagcgtgata gattgggtca aattcttgtt ccaaacggtt
     8461 taattcctca agtagtagtt tgaggttgct ccgagtgcgg tacacattgg cgtataacgc
     8521 cattttctct cgaatagtag aaagcacttc actggcagtt agttctagat tagcagttga
     8581 attagcaagc tgatttaatg ctttttctgc cattgcaagc ggtgagattt cgctgttttt
     8641 ttgcaaatca ttctgttcta gcgaacgaac aatttccgat accgcacgtt tggcaaacac
     8701 taagccaccg ccgactgagt taccacccat acggtttgcc ccttcaatgc agtgagaaat
     8761 ctcaccaatc gcaaatagcc cttttactgc actttcgccc cattgattaa tttgaatgcc
     8821 gccgttacag ctatgagcaa acggcttgat tgcaatctta tcttgcacta aatcaatccc
     8881 gacttcattt ttgagccaat tcaaatagac ggtataaaac tcttccgtgt cttcatacaa
     8941 tttggggctg tatttaaggt aaatgccttt ttcgtgaggg ttttcaagca aatgtttcat
     9001 catcactaaa tcgaactcca cgccggcaaa atcaaggctg aaaggagcat aatggctacg
     9061 ttccagcacc atttttgcaa attgctcggc atttaagtgt ggaaatagat tgttaccctt
     9121 agaatcggtg atttgggtga catacttcaa tgtatgttcg ccaaatagca ccttgtattt
     9181 tggctcgaca aaggcaggaa tgaactgaat aaaggcaaga ttaaccaagg ttgccccggc
     9241 ttgttgtgcc acataatgaa gtgaaccgat aacatcagcc gggtagagat tatctttata
     9301 cagcccagca atcccgcctg cggctaaaat aagatgcttg gttttgcaaa aaatatagga
     9361 aatttgaccg cttgtagtga gcgaaaatac cgcaccttgc acttggttat tgtctgtgac
     9421 gatgtgtaaa agggtagcat tttcatagaa atcaatgtgt tgctcttgca aaatctgttt
     9481 cgctcgtagg gcagattccc gccaatcttt aattaaataa attgggcgag ggtatttggc
     9541 aaaacaagcc ggacggttgt cgttacgctt ccaaggctga aagccgatac ggtggagtag
     9601 ttcaatcgct tgtggtgaat cttcgatata ggcttgtacc acattagggt tgattttgcc
     9661 tttgccgatg ttttcaatat cttctttaaa gtgaatttta tcggcttcat cgccggttac
     9721 ttgaatgcca agtgttgcct taagcggaaa gtagctcgct cccgagccaa tcgtagattt
     9781 gctaatcagt aaggtattaa tgcctttttc ttttgcctca actgcagcag aaagcccagc
     9841 gatgccagag ccgacaatta ataaatcaat gttaatggtt tgggtaattt ggatcataaa
     9901 taaaaacaca agcgggtaaa atttgcgaaa aattttaccg cttgtaggca caaagcacaa
     9961 ttataataaa gatggtgctg cttcaattaa ggttcgggtg tactcttgcg ttgggcgttc
    10021 aaatacgtct gaagcatcgc ccatttccac aattttaccg ccgttcataa ccacaatgcg
    10081 gtctgaaact tggtgaacgg tttgtaagtc gtgagagata aataccatcg ctaaattgag
    10141 ttcttttttc aagtctgcca gcaggttcag tacttgagct cgaaccgata cgtcaagtgc
    10201 agaggttggt tcatcagcca caatcagctt cggatttaat gccaatgcac gagcaatcgc
    10261 tacacgttgc ttttgcccac cagataatcg ggttggctcg accaacgccg ctgaacgagg
    10321 tagaccgact cgagagagca aatcataaac ccgcttctca cgttcttgcg gttgtaagat
    10381 attatgaata tccatcggat cttttaaaat atccaacaca cgcatacgcg gatttagggc
    10441 ggttgccggg tcttggaaaa ttactgacac ttggctaccg aaatatttgc gagcttccgc
    10501 actgccatat ttcatctcta agccgttaaa ttttactgtg ccggaggttg gtttttgaag
    10561 cccgatcatc acactggcta aagtagattt gccacaacca gattgtccaa ccagaccgac
    10621 tgtttcaccg gcgtgaattg ctaggctgac atcatttacc gccgtgaatt ttttcgggtt
    10681 aaataaggtg ccgtcacgag aagcaaattg cacaacaata ttagacagtt caataattgg
    10741 ctgttctttg ataaatttca tactcatcgt attgctctcc tttatgcttc tttagattgt
    10801 ggttcttcta agtgtgaagc ccagaagtgt tgtgggtgat caccggttgg cactaatttc
    10861 aagtgctgtt cagggtttgc atctggtctt aatgaacgac tcgcaaaacg gtcacctttc
    10921 gcaaaatcaa atggtgaagg cacactgcct ggaatttggt ataggcgttc tgcacgcaat
    10981 tcggtagaaa gtaccgagcc taataatcca cgtgtgtatt cgtgtgttgg gtttgccaat
    11041 aatggcgtag ttggtgcagt ttcaaccact tgaccagcat acattaccgt gatgtggtga
    11101 gcgatttgtg ccactaaagc taagtcgtgg ctcacgaata ccattgcaaa tccaagtttc
    11161 tcacgtaatt cgtttaataa tttgatcact tcagcttgaa cggttacgtc taatgctgtt
    11221 gttggttcat cagcaatcaa tagtttcggt tcacgagcaa gtgccattgc aattaataca
    11281 cgctgacgtt gtcctccaga taactcgtga ggataacggt tgagcgtttt ttccgggtca
    11341 agttttaccc attgcaataa tgtttctgct gattgtttac caccacggct gattaattgt
    11401 gccatttggt ctttaatacg cattgatggg tttaacgcac ttaatgcatc ttggtaaatc
    11461 atcgagattt cgtgaccacg aagggcattt aaatcggctg attctaaaag attgtgttgt
    11521 ttacctgaac ggtcggtaaa gaggatttca ccagtgattt ttgcagtttt tggtaataaa
    11581 cccataattg agaatgcggt gattgattta ccacaaccag attcacccac taagcccatg
    11641 gtttcgcctt cgtgaacagt aaagctgata ttatcaacaa gcggagtttc accataacga
    11701 tttgggaaac ggattgataa gtttttcact tgtaaaatcg gtttggccgt tgagtttaat
    11761 tgcatacggt cagtacgggt cgtttctttc tcgtgtaatt tgagtaagta acgttttaac
    11821 gcaagagttt cagacattgc ttcttgaacg tctgttgcca atggttttgc gacatcttct
    11881 ttactttgag ttgaacgttt tagttttgga tttactaacg catcggttaa gccttcagca
    11941 aggatattta acgcaagtac ggttaataaa atcataatcc ctgcgaaagt tgtagcccac
    12001 cagaagccac ttaatacaag gttacgacct tcagataaga tatttcccca agatgggaat
    12061 ggaggctgga cacctgcacc taagaatgaa agtgatgctt caaatacgat ggcatcagca
    12121 accatcactg ttgcgaatac taaaaccggt gcagcagtat tacgcacaac gtgttttaat
    12181 agaatataag tacggctacc accaattaca cgctcggcac gcacataatc ttcttcccat
    12241 tgagatacta cgttagcacg caccactcgg gcaagttggg gagtgtaaac aaccgcaata
    12301 gtcacgataa tcacaggtac agagttaccg aaagtggcaa gtaatacagc agctaatgcg
    12361 atacccggga atgccattaa gatatccata aaacgcataa tcacttcgtt accgaattta
    12421 tcggcggttg ccgcggttgc ccctaaaata ctaccgaata gaatcgcaac gcctaccgca
    12481 cctaaaccga taaagagaga tgttcttgct ccccatacca tacgcgagaa aatatcacgt
    12541 cctaaacggt ctgtaccgaa taaataatca ccgcttggag cttgcactgg acgtagcgtt
    12601 tgtagtgggt cataaggcgc tactaatggt gcaaaaatag cgactaatgc gataaataca
    12661 ataaagagta aggcgatttt tgagctggtt gataatgctc taaatcttgc accgcctgaa
    12721 gcaagtcgag cagctaatcc ttggcgaaac atattataaa ctccgaattt ttggattaat
    12781 gagcaagtaa agaatatcaa caatgatatt caccaacacg aaagtaaatg cgatagttaa
    12841 tactacacct tgcactaagt tcaagtcgtg attgacgata ccgttgaaaa ttaatttacc
    12901 cattcccggt aagtcgaaga tctgttcaat cacaactgca ccacctaata aataaccaac
    12961 ccgtaaacct aatactgtta ccggagtaat taaggcatta cgaagcacgt tgtggcgaat
    13021 caccgttgag taaggtacac cattaccgat ggctgttcga acataatctt tatccatttc
    13081 ttctaccatt gttgtacgca cgacacggat caatgaggca catacaggaa cagctaaagc
    13141 gagtgaaggg agaaccattg agtttacata gccttttgga tcttcgctaa atggaataaa
    13201 accgcctgaa ggcaaccaat ctaattttaa ggagaagtat tgaatcaata aaatacccaa
    13261 ccagaaagaa ggtgtggcaa cagcggcaac cgagattaaa cggattaatt gatcaatcca
    13321 agtatcacgg taaagtgctg ccgttacacc taatgtgaaa gagacgatag cagctaagaa
    13381 gaccccaata aaggtaagct gtaatgttaa agggaatgct ttagcaatca tatcaacaat
    13441 aggctgttct ggaggggttg tcataccgaa atctaatacg aataaattcc ctaaaaagcg
    13501 aaaatattgt acaagcaatg gatcatttaa cccatgttgt tcacgataga tttctttagc
    13561 ggcttcgctg gcgctttcac cgagagcaac ggttgccgga tctccggggg tgaattgtaa
    13621 aacaataaat accaaggcag ttacgcctaa gatcattacc ggcagagcca ttaggcgacg
    13681 ccataataat cggagtataa tttccatttt cttctccttg agtgtagtta cactccaaaa
    13741 ttaaatctaa gtttacctac aagctaatat agtgatatgt aggattataa atgaaaatat
    13801 agaaatggta agaattctga gtgatataag acgaatatta catcattttg atttttattt
    13861 atgcaagcgg taaatttttt gtaaaatatt accgcttgca tttagctagc cgttacgact
    13921 atttagcacg accaacaccg ataaatgaca tacctgttgt tggtaatggt tggaaacctg
    13981 ttaatgcctt atcattccac gcagttggca atttacggtg aataattggg taaagaggca
    14041 cttgttcagc gataatattg attgctttcg cccaggcttc tttagaagca gcaggatttt
    14101 tggccgcttc atcaagcagt ttttgtactt ctgcatattc agctgtttca gaccagcgga
    14161 aacgacgttt cggccataca tcaccacgat accaccagct taataatagg tctaagtcat
    14221 taccgaaaac tgatgggtcg ccaggtgcaa tcacaacttc gtatgcacct ttatcaacgt
    14281 gtgtgccata taatgcacct gattgtaagt gtttgagtga aactttcacg ccttttaagc
    14341 tgttccacga ttcaagaatt aatggcgcac attctttcac ccaagagtga tcggttgcca
    14401 acaattcaaa ttttaactct gtcacacccg cttctgctaa taacgcttcg gctttcgctt
    14461 tatcgaaatc atattgcgaa tttgctttca cataatccgg gtgagtatct tgcacgtaag
    14521 atgtagcagc ttttgcattg cctaagaaca ccacatcaat taatttttgg gtatctaagc
    14581 cataatgtaa cgcttggcga actttcgggt tgttaaacgg tgctttttca cagttaaaca
    14641 ttaagaacag taagccgaat gattgtactg actcaacggt acctttacgt tttaagcgtt
    14701 ctgcatctaa gtaaggcaca gactcaattg cttgaacacg gccagattct tgagcggtta
    14761 cacgagcggc atcatcagaa agtaagaacc aagtcatttt ctcgacttgt gccgggtagc
    14821 caccgttata atctgcaaat gcttcgaaaa caatacgatc atctttagtt gcggatacaa
    14881 atttatatgg gccagtcccc actggattag catcaaatgc agcttgaccg gcttctacca
    14941 ctgcttttgg tacgatttta acaatggtta ggcgctcttt gaataacgca aatggatatt
    15001 tcaatttgaa ttcaaccact ttgtcatcaa cttttttcac agtgtcaata aatggaatga
    15061 attgtgcgaa aagtgatgct ttagctgggt ctaatacacg ttcaaatgag taaactacgt
    15121 cttcagtggt aaccggatta ccattgtgga atttagcccc gtcacgtaaa gtaattcgat
    15181 aggttacatc atcaattttt tcaggctctg ctgctgccaa tgctaaatat ggtttgcgag
    15241 tggctggatg taagtccacg aggccttcaa aaatatggag gtttgccgcc attgaggatg
    15301 caccgcttga ggtaagcggg tcaaatccgg ttgatatggg ataagctata ccggcctcaa
    15361 tcgttgagcc ggcggcaggg gctgcaaatg ctttaggtgt aaatgtacct aaagtgccac
    15421 taaatgccat accggcaccg acacctgcaa ttagtttcat aaaaccacgg cgggattcgt
    15481 tgtgttcaaa ctgttttttc atcttaaagc tcctttaagt ttttatgata ggggcggtat
    15541 tatttgctga agtgaattta tcaaaaaatg cacttttttt aaattgaatt tatcatatca
    15601 ttaatgtcaa agtggtaaag caagaattat tgaatttgtg atctagctca cgaaaatctt
    15661 tgcttttcac ttcacgtgct tttcgttatt attcgccaat ttaatcataa atgaaaaatt
    15721 gacagtgaac ctgattttac cgcctttaag cctttatatc catatccctt ggtgcgtgca
    15781 aaaatgccct tattgcgatt ttaactcgca cgctcaaaaa ggattaattc ccgagcaaga
    15841 atatatcgaa catttacttg cagatttatc ccaagatttg accgcttacc gagtggcaat
    15901 aggtgatcgt aaattatatt ctatttttat tggcggcggc accccgagct tattttcagc
    15961 ggaagggata gcttatttat taagtgaagt agaaaaacgg attgcattcg agccaaatat
    16021 cgaaattaca ctggaagcga acccgggaac cgcagaggca gagcgatttt taggttacgc
    16081 acaaggtggc gtaacccgca tttcaatggg aatccaaagt tttgagccgg aaaaattatt
    16141 gaaattagga cggattcacg atgcaaatga agcaaaacaa gcgattaaat ttgcccaaaa
    16201 ttctgcaaaa tcgggcttgc aaagtttcaa cattgattta atgcacggct taccaaacca
    16261 atcggttaaa caagccttgg tggatttaga aacagggatt tcactcaatc caccgcattt
    16321 atcgtggtat cagctcacga ttgagccgaa tacgatgttc tattatcgta agccaacctt
    16381 gcctgatgat gacgagttat gggatatttt cgagcaaggg catcaacttt taacgaatgc
    16441 cggctatgag cagtatgaaa cctcggctta tgccaaaaaa ggctaccaat gcagacacaa
    16501 tttaaattat tggcgatttg gggattattt ggcgattggt tgtggtgctc acggcaaaat
    16561 ttcgtatccg acaggggaaa tttatcgttt tagcaaaacc aaacacccga aaggttatat
    16621 gcgaggggag taccgctaca gccaagatct gattgagtta gacgaccgcc cgtttgagtt
    16681 ttttatgaac cgctttagat tgcttgaagc cacgccaaaa caagaatttg aatggtttac
    16741 cggtttagac agggaaattg ttcgcccgac aatcgattgg gcgttaaacc aaaactatat
    16801 ttccgaaagc tcaacccatt ggcaaattac ccaacacggt aaattgtttt taaatgaatt
    16861 actggaaggc tttctagact gatttgcaaa aaaacgacca aaacagaccg cttgtaatgc
    16921 taaattacag gcgttttttg tgatgtgcgt cgaaaaattc gtatttttta ctagacaaaa
    16981 aaacagtatt cagtagcata gctctcacaa ataaggagag cttatgaccg ataaacaaat
    17041 ccgtaatagt accgccgaat ttttgatttt tacccaacaa aatggagcag aaaatattac
    17101 tgttcgttat gaagataacg aaatttggct cacgcaaaaa ttaattgctc agctttttga
    17161 tgtttctatt ccaactatca atgagcattt aaagcatatt tttgaaagtg gagagctaga
    17221 agaaatgtca gttattcgga atttcctaat aactgccgca gatggtaaaa attatcgtac
    17281 caaacactac aaccttgatg ccgttatttc gataggctat agggtaaact cggtgcgggc
    17341 gacacaattt cgccaatggg caactaaagt attacgggat ttcgcccaaa aaggctatgt
    17401 gattgaccgc caaagaatgg aaaatggtac atttttaaat gaagattatt ttgagcagtt
    17461 gctggcagaa attcgtgaaa tttggttatc tgaacggcgt tcttgagggc aaaggcaaaa
    17521 tgcgttttga tgaagcgaaa ttgcacgcag aaagcgaatt tgagaaatac cgcattgttc
    17581 aagacagatt atttgaaagc gattttgaca aggtattgaa agaagtgttg cggtcggaaa
    17641 atcaataccg tccatcattt tcaacaacat aaaacaaccg cattttgcaa attttttgcc
    17701 aagtttgacc gcttgccgtg tataatgcaa gcggttattt tttatggaaa aattacaaat
    17761 gaatatctta caagcaggcg ataaagcccc tcaattcact cttcaaaatc aggctgatga
    17821 gtcggtttca ctctctcaat ttgccggtaa aaaagtgttg gtttatttct acccgaaagc
    17881 ccttacaccg ggttgcacta cgcaagcttg tggtttgcgt gacagcaaat cagaacttga
    17941 tgagctcaat gtggttgttc tcggaattag caccgattta ccgaaaaaat tagctcaatt
    18001 tgtagagaaa aaagccctta attttacctt attatctgac ccggaacacc aagttgcgga
    18061 agcctttggt gtatggggcg aaaagaaatt tatgggaaaa acctatgacg gcattcaccg
    18121 catcagcttt ttaattgatg aacagggcgt ggttgagcaa gtgtttgata aatttaaaac
    18181 cggcgaacat caccaaatga tcgtggatta tctacggggt cgctaatggg aaaaaaaacc
    18241 atttttttag accgtgacgg cacaattaat atcgatcacg gctatgtgca tcaaattgat
    18301 gattttcaat ttattgacgg tgtcggcaag gcgttaaaac agctacaaga caaaggttat
    18361 ttattggtgc tggtaaccaa tcaatctggc attgcccgag gctattttag cgaagcacaa
    18421 tttatgcaac taaccgaatg gatggactgg tcgctcgatg aagattatgg cgtggtacta
    18481 gacggtattt actactgccc acatcacccc gaaggcaaag gcgaataccg tgaagattgt
    18541 gattgccgta agcctaaagc aggtatgttt actcaagcga ttcaagattt aaacatcgac
    18601 ccaagccaat cttatatggt gggcgataaa ttagaagata tccttgccgc agaagctgcc
    18661 ggcgtgaaaa ccaaagtgct ggttcgcacc ggcaaagccg tgacggaaga aggtcaggct
    18721 aaagccgatt tggtgttaga tagtttagtt gatttagtaa aatatattaa ataaagcaat
    18781 gaattataac gataaatctc tttcagccct gaaattaggg caaaaaaccg agtacaaaag
    18841 cgaatatgac gcctcgcttt tgcaacctgt tcctcgtaaa ttaaaccgtg atggtttagg
    18901 cattacacaa gagcagcctt ttaaccgagg ggctgatgtg tggacttgct atgagctatc
    18961 ttggttgaac caaaatgggc taccgcaagt agcgattgca gatgtcgcca ttgattttaa
    19021 gagcgaaaac ttaattgaat cgaaaagttt caagctctat ttgaacagtt ttaaccaaac
    19081 caaatttagc tcaatagaag aagttgagaa aacgattcaa gcggatctgg ctcgctgtgc
    19141 aagcggtcag gtttcggtaa aaatttacca attatcgcac tacacgcaac agccgatagt
    19201 tgattttgca ggcgattgca ttgatgagca agatattcaa atcgacagtt atgcgttttc
    19261 gactcaacat ttagaaaaca ttgcagaaaa tgagccggtg gaagaaatct tagtcagcca
    19321 tttgctcaaa tctaactgct taattacttc acagcctgac tggggaagtg tgcaaattcg
    19381 ctacaaaggc aaaaagctaa actgtgaaaa actgttacgt tatttagtgt cgttccgtga
    19441 gcataatgag ttccacgaac aatgtgtgga acgcattttc tgcgatttaa tgcaattcgc
    19501 tcagccggaa aaattaacgg tttacgcacg ctacaccaga cgaggcgggc tagacatcaa
    19561 tccgtttaga tcgaattttg aggaagtgcc ggaaaatcta agaatggcac gccaatgatt
    19621 ttgttgtttt ttgttcccgt ctgaaccgcc tgagcgtgtt tgaattggta ggtaaattta
    19681 ctctgagcgg agcgaatgta aatttgccgt taagccaatt caaactaagc gaggatagcg
    19741 gtgaagctcg ggttgccttt cttttggtta cttttctttg gcaaagcaaa gaaaagtaac
    19801 aaatagcaga tgttgatttt tatgatgaat gaatatagcc caaattacta tataggcgta
    19861 atgtccggca cgagccttga tggggtggat attgctttgc tggattttgc aaaaaatcca
    19921 ccgaaaatga ccgcttgtga tttttttcca atgccggagg aattacgggc ggatatttct
    19981 gcgttactta aaaccggcga aaccaatctc caaaaactgg gggaaattga tcaccgttta
    20041 ggcttgcttt atgcagaaac gatcaatgcg tttttagcga aaaatcagct aaaagcagag
    20101 gatattcaag ccataggttg tcacgggcag acggtgtggc attcaccgga ttctgagtat
    20161 cccttcacca cccaaattgg cgatatgaat ttggtggcag agcaaaccgg tattaccact
    20221 atcgctgatt ttcggcgtaa agatatggca gtaggtgggc aaggggctcc gcttgtgccg
    20281 gcatttcatc aagctatttt tagcgatcca aatcgtctaa cagtggtgct gaatattggc
    20341 ggcatcagca atatttcggt attaaatccc aattccccta cagttggtta tgatgtagga
    20401 gccggaaata ctttgatgga tagttggatc gaacaacatc aaggcaaaag atacgataaa
    20461 aacggcgaat gggcgagaac gggcaagctc aatactgaac tgttagcgac gttattagac
    20521 gagtcgtttt ttgctaaagc cccgcctaaa agcactgggc gggagctgtt taatttaacg
    20581 tggcttgcaa aaaaattgga aaatttgacc gcttgtcagc cacaagatgt gcaacgcacg
    20641 ctggcagaat ttactgctca aagtattgcc aatgagttat tacgctttga aagctctctg
    20701 cctcgcttgc ttttagttgg tggtggtggg gcaagaaatc cgttattaat ggaacggatt
    20761 tctgctttac ttcccgagtg ggaggttaca accactacag aatttggttt agatattgat
    20821 tatgtagagg ctgccgcttt tgcttggtta gcttaccagc gaatccataa tttacccagt
    20881 aatatgccta gcgtgacagg agcaaaaaaa gccgtgagtt tgggcgtgat ttaccctgct
    20941 tagattaaga aaatgtaagg taatacattc attactccga gagtggtaat agctaaaatt
    21001 aatttagagc ttccaaacat taagccttgg tttttaccat tgtctaattt cactagcata
    21061 ttagatagtg ccgtattcgc atacacggta atagtggcaa aaatcactaa atagagaaat
    21121 gcagtgatgg ttgaaagcca ttggagtgca taaaaccaag gcattatttg aagtataatc
    21181 agtattggtg caatataaat aagtttatgt actgtcagat ttaattttcg tgaaaaatca
    21241 gattgcaata acactgcaat taaattattc acaaagaatg agacagagac taattctggt
    21301 ttatccagaa gattggtgaa tatatagggg aatataatat aaaatgcact tcctagacca
    21361 agcggaataa aaag
//
LOCUS       DANKBV010000031        21239 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.031, whole genome shotgun sequence.
ACCESSION   DANKBV010000031 DANKBV010000000
VERSION     DANKBV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21239
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.031"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            1..290
                     /locus_tag="PWY43_000905"
                     /pseudo
     CDS             1..290
                     /locus_tag="PWY43_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250010.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(375..1724)
                     /locus_tag="PWY43_000906"
     CDS             complement(375..1724)
                     /locus_tag="PWY43_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL2135800.1"
                     /translation="MLLFIMFAAIILLLVLIMKFKVHAFVALIIVSLLTALAAGIPVD
                     KILPTLLNGFGSTLASVALLVGLGAMIGRLLEITGGAKVLADTLINKFGKEKAPFTLG
                     VASLLFGFPIFFDAGLVVMLPIIFSVAKQFGGSVLRYAFPSAGAFAVMHAFLPPHPGP
                     VASGDLLGVNMGLLVLVGLVCAIPTWYIGTYLFSQFISKRIHVDLPKAFLNGLSANEM
                     AVQNPPSFGKVLTVLVLPIILILFDTGLNTLSVAKVIDGSEMWVQSLRLIGKTPVALL
                     ITLIVAIMLLRDNRSFDQIEKICNNALGPICSIVLVTGAGGMFGGVLRASGIGDVLSE
                     MMKDTGMPIVVAAFIIATVFRVAQGSATVALTTTAALIAPTVAAATDLSQFDLCFIVI
                     AIASGATVLSHVNDSGFWLMSRFLEMDEKTTLKTWTMLETSIGLTGFACALIGSWLL"
     gene            1911..2426
                     /locus_tag="PWY43_000907"
     CDS             1911..2426
                     /locus_tag="PWY43_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL2135801.1"
                     /translation="MANGKAFILMGVSSTGKTSVGTAVAQRLGIKLIDGDDLHPRANI
                     LKMASGTPLNDADRTPWLERINDAVFSFEQKSEKGIIVCSALKKKYRDQIRQGNADVT
                     FLFLHGSFDLVLARMQQRKGHFMKPEMLQSQFNTLEIPQADEPDVIFIDIDGSFDEVV
                     ERCIKAAEPLI"
     gene            complement(2480..3478)
                     /gene="gntR"
                     /locus_tag="PWY43_000908"
     CDS             complement(2480..3478)
                     /gene="gntR"
                     /locus_tag="PWY43_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDL2135802.1"
                     /translation="MSKPKRPTLQDIASHLGITKMTVSRFLRDPNTVAKETGERIAQA
                     IELFGYIPNRAPDILSNAKSRAIGVLVPSLTNQVFADVIKGIEQITDQAGYQTMLAHY
                     GYSEQKEEQRIESLLSYNIDGIILSENHHSPRTLKMLEVANIPVIEIMESGECGIQQA
                     VGFDNISAAQAMVETMIHRGCRQVVYFGARMDKRSQLKMQGYEQAMLKHGLSPHSITT
                     AEHSSFTLGASQLKQALEQIPQLDGIFCTNDDLAIGALFECQRLGINVPQQIKIAGFH
                     GHDVGQSLTPQLASVMTPRLEMGRVAAQELLDRIHGVPQQSPIINLGYHIHLGESV"
     gene            complement(3571..4731)
                     /locus_tag="PWY43_000909"
     CDS             complement(3571..4731)
                     /locus_tag="PWY43_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDL2135803.1"
                     /translation="MIEQSYKIALLFNANKIYDRGVMQGIGQFIQASQCTWDIFVEDD
                     FVYHKNSIESLLIDGIIADFDDLETVELLKDIDVPIVAVGGSYQNSDYYPNLPYVATD
                     NYALIEAAFLHLKQKGIDQFAFYGYPAETEKHWAIERQNAFIQLMEKYEHQPNCYIGD
                     QFHSDNWVELQAKLGEWLQTLPPHTGIIAVTDARARHLLQTCEHLKIAVPEQLCVIGI
                     DNEELIQYLSRVSLSSVTQGTSQIGYHAAKLLYRKLNNLPVSLKPILIPPVKVEERRS
                     TDYRSLQDPMVIQAMHFIRHRACQGIKVEQVLDHLKVSRSNLEQRFKEEMNKTIHQVI
                     HEEKLARAKYMLKFTEISTQEIANVCGYPSLQYFYAVFKKEFGKTPKEFRQE"
     gene            complement(4854..5240)
                     /gene="rplQ"
                     /locus_tag="PWY43_000910"
     CDS             complement(4854..5240)
                     /gene="rplQ"
                     /locus_tag="PWY43_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL2135804.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMASALVEHEIIKTTLPKAKEL
                     RRVVEPLITLAKADSVANRRLAFARTRNVDTVAKLFNELGPRFAQRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRPDVATEAAAE"
     gene            complement(5276..6265)
                     /gene="rpoA"
                     /locus_tag="PWY43_000911"
     CDS             complement(5276..6265)
                     /gene="rpoA"
                     /locus_tag="PWY43_000911"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL2135805.1"
                     /translation="MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVQNKDDIILTL
                     NKSGIGPVTAADITHDGDVEIVNPDHVICHLTDKNASINMRIRVQRGRGYVPASARLH
                     TQNDDRPIGRLLVDARFSPVDRIAYNVEAARVEQRTDLDKLVIEMETNGTLDPEEAIR
                     RAATILAEQLAAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAETIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIAED"
     gene            complement(6296..6922)
                     /gene="rpsD"
                     /locus_tag="PWY43_000912"
     CDS             complement(6296..6922)
                     /gene="rpsD"
                     /locus_tag="PWY43_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL2135806.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIESKCRNRLDVAPGQHG
                     ARKPRLSDYGSQLREKQKVRRIYGILERQFRNYYTEANRLKGNTGENLLVLLEGRLDN
                     VVYRMGFAATRAEARQLVSHKSIVVNGRVVNIPSFQVSVDDVVAVREKSKKQARIKAS
                     LELATQREKPTWLEVDATKMEGVFKRRPERSDLSADINEHLIVELYSK"
     gene            complement(6951..7340)
                     /gene="rpsK"
                     /locus_tag="PWY43_000913"
     CDS             complement(6951..7340)
                     /gene="rpsK"
                     /locus_tag="PWY43_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL2135807.1"
                     /translation="MAKTPVRARKRVKKQIADGVAHIHASFNNTIVTITDRQGNALAW
                     ATAGGSGFRGSRKSTPFAAQVAAERCAEMVKEFGLKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(7357..7713)
                     /gene="rpsM"
                     /locus_tag="PWY43_000914"
     CDS             complement(7357..7713)
                     /gene="rpsM"
                     /locus_tag="PWY43_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL2135808.1"
                     /translation="MARIAGINIPDQKHTVIALTAIYGIGKTRAKAICAATGIAEDVK
                     IRELSEEQIEKLREEVGKFTVEGDLRREVTLSIKRLLDLGCYRGLRHRRSLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(7851..7964)
                     /gene="rpmJ"
                     /locus_tag="PWY43_000915"
     CDS             complement(7851..7964)
                     /gene="rpmJ"
                     /locus_tag="PWY43_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL2135809.1"
                     /translation="MKVRASVKRMCRNCKVIKREGVVRVICSDPKHKQRQG"
     gene            complement(7989..9308)
                     /gene="secY"
                     /locus_tag="PWY43_000916"
     CDS             complement(7989..9308)
                     /gene="secY"
                     /locus_tag="PWY43_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL2135810.1"
                     /translation="MAKQPGYQGSTQKGTGELKSRLLFVLGALIVFRIGSFIPVPGID
                     ASVLSELIRQQQGTIIDMFNMFSGGALSRASIFALGIMPYISASIIIQLLTAIHPPLA
                     ELKKEGEAGRRKISKYTRYGTLLLAFIQSIGISLALPNMVHGLVPNPSILFYVTSVVS
                     LVTGTMFLMWLGEQITERGIGNGISLIIFAGIVADLPATIGQTIEQARQGELSFIVLL
                     LVAIIAFAVTYFVVFVERGQRRIVVNYASRQQGRMMAPARTSHLPLKVNMAGVIPAIF
                     ASSIILFPASITQWFGQSEGFEWLFNLSQLLQPGQPLYVVLFTMAVIFFAFFYTGMQY
                     NPRDTADNLKKSGAFVPGYRPGEQTSRYIDKVMTRLTLIGALYIAFVCLVPYIITSLW
                     KVPFQLGGTSLLIVVVVIMDFIAQVQSHLMSQQYDSVLKKANLKGLN"
     gene            complement(9311..9745)
                     /gene="rplO"
                     /locus_tag="PWY43_000917"
     CDS             complement(9311..9745)
                     /gene="rplO"
                     /locus_tag="PWY43_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL2135811.1"
                     /translation="MRLNSLSPAEGAKHSAKRLGRGIGSGLGKTGGRGHKGQKSRTGG
                     GVRRGFEGGQMPLYRRLPKFGFTSLKSFHVAEIRLNDLAKVDGNEVTLETLKAANIIT
                     KDILSAKVILAGKIERAVVVKGLRVTKGAKAAIEAAGGSIEE"
     gene            complement(9750..9926)
                     /gene="rpmD"
                     /locus_tag="PWY43_000918"
     CDS             complement(9750..9926)
                     /gene="rpmD"
                     /locus_tag="PWY43_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL2135812.1"
                     /translation="MAKIKVTQTRSSIARLPKHKATLKGLGLRHIRHTVELEDTPAVR
                     GMINQVSYMVKVEE"
     gene            complement(9933..10433)
                     /gene="rpsE"
                     /locus_tag="PWY43_000919"
     CDS             complement(9933..10433)
                     /gene="rpsE"
                     /locus_tag="PWY43_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL2135813.1"
                     /translation="MSNIEKQAGELQEKLIAVNRVSKTVKGGRIMSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNEGTLQHPVKGSHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVRNVLSKAYGSTNPINVVRATIDALENMKSPEMVAAKRGKT
                     VEEILG"
     gene            complement(10448..10801)
                     /gene="rplR"
                     /locus_tag="PWY43_000920"
     CDS             complement(10448..10801)
                     /gene="rplR"
                     /locus_tag="PWY43_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL2135814.1"
                     /translation="MDKKVARIRRASRARHLMREQGATRLVVHRTPRHIYAQVIAPNG
                     SEVLAAASTVEKVIKEQVKYTGNKEAAAVVGKIVAERAIAKGIKAVAFDRSGFKYHGR
                     VQSLADAAREAGLQF"
     gene            complement(10816..11349)
                     /gene="rplF"
                     /locus_tag="PWY43_000921"
     CDS             complement(10816..11349)
                     /gene="rplF"
                     /locus_tag="PWY43_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL2135815.1"
                     /translation="MSRVAKAPVNIPAGVEVKLNGQLLTVKGKNGELSREIHNAVEVK
                     QEADALTFAPREGIANADAQAGTARALVNNMVIGVTEGFTKKLQLVGVGYRAQMKGNA
                     VALSLGFSHPVEHTLPAGVTGECPSQTEIILKSADKQLIGQVAADIRAYRKPEPYKGK
                     GVRYSDEVVRTKEAKKK"
     gene            complement(11366..11758)
                     /gene="rpsH"
                     /locus_tag="PWY43_000922"
     CDS             complement(11366..11758)
                     /gene="rpsH"
                     /locus_tag="PWY43_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL2135816.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKVAISMPSSKLKVAIANVLAEEG
                     YIESVKVVEGVKPELEITLKYFQNKPVVESIQRVSRPGLRIYKRKDELPKVMGGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(11795..12100)
                     /gene="rpsN"
                     /locus_tag="PWY43_000923"
     CDS             complement(11795..12100)
                     /gene="rpsN"
                     /locus_tag="PWY43_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2135817.1"
                     /translation="MAKQSMIARDVKRAKLADKFYAKREELKKIISDVNASDEDRWAA
                     VLKLQTLPRDSSPVRQRNRCRQTGRPHGVLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(12112..12651)
                     /gene="rplE"
                     /locus_tag="PWY43_000924"
     CDS             complement(12112..12651)
                     /gene="rplE"
                     /locus_tag="PWY43_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL2135818.1"
                     /translation="MAKLHDYYRDTVVNELKAKFNYSSVMQVPRIEKITLNMGVGEAL
                     TDKKLLDNAVADLAAISGQKPLVTKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLNAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTSA
                     KTDEEGQALLAAFNFPFRK"
     gene            complement(12669..12980)
                     /gene="rplX"
                     /locus_tag="PWY43_000925"
     CDS             complement(12669..12980)
                     /gene="rplX"
                     /locus_tag="PWY43_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL2135819.1"
                     /translation="MAAKIRQNDEVIVLTGKDKGKRGKVTKVLPNGKVFVEGINIITK
                     HEKPVPALGKAGGLVKKEAAIDVSNVAIFNLETNKADRVGFRFEDGKKVRFFKSSEKT
                     I"
     gene            complement(12991..13362)
                     /gene="rplN"
                     /locus_tag="PWY43_000926"
     CDS             complement(12991..13362)
                     /gene="rplN"
                     /locus_tag="PWY43_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL2135820.1"
                     /translation="MIQEQTMLDVADNSGARSVMCIKVLGGSHRRYAAIGDIIKVTVK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGAVIRFDGNACVILNNNTEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(13585..15420)
                     /gene="glmS"
                     /locus_tag="PWY43_000927"
     CDS             complement(13585..15420)
                     /gene="glmS"
                     /locus_tag="PWY43_000927"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL2135821.1"
                     /translation="MCGIVGAVAQRDIAEILVDGLHRLEYRGYDSAGVAVLNADDKTM
                     QIVRRVGKVQALDEALDTKPLLGGTGIAHTRWATHGEPSETNAHPHRSGKIAVVHNGI
                     IENYEELKIVLQERGYVFQSQTDTEVIAHLVEWELRSASSLLEAVQKTVVQLRGAYGT
                     VVLNEEEPEHLIVARSGSPLVIGYGVGENFLASDPLALLSVTRRFAYLEEGDVAEITR
                     RTVDIYDREGNKVEREIHEGNFEADAADKGQYRHYMQKEIFEQPVAIMNTLDGRIKDG
                     KVDISAIAPNAAEILSKVEHVQIVACGTSYNAGMVSRYWFESIAGVSCDVEIASEFRY
                     RKFVTRPNSLLITLSQSGETADTLAALRLAKESGYMAAMTVCNVASSSLVRESDFAFL
                     TKAGVEIGVASTKAFTTQLTCLLLLNVAIGRLQGRMSEEQEHHIVQSLQRLPAQIESA
                     LVFDKQIEKLSEDFADKHHTLFLGRGEYYPIAMESALKLKEISYIHAEAYAAGELKHG
                     PLALIDSDMPVVVVAPENDLLEKVKSNIEEVRARGGQLYVFADHDAGFEEAEGFKTIV
                     MPKVDEVTAPIFYTVPLQLLSYHIALIKGTDVDQPRNLAKAVTVE"
     gene            complement(15468..16229)
                     /locus_tag="PWY43_000928"
     CDS             complement(15468..16229)
                     /locus_tag="PWY43_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL2135822.1"
                     /translation="MSKRNTQQRRHLIVQMVQEKNEVSVDELAHFFETSEVTIRKDLT
                     ALEESGFLLRKYGGAVKIPSEMMEEAFIEQLSIQKKMIAQAARDCIRDHNRIIIDSGS
                     TTGALVKALNQTGLVVMTNSLNLAAELTSLECEPTVLMTGGTWDSRSESFQGKVAEQV
                     LRSYDFDQLFIGADGLDLARGTTTFNELVGLSQVMAEVSREVIVLIESQKIGRKMPNI
                     ELEWQNITKIITDSQLSFETKEQIEKMGVEVVIAK"
     gene            complement(16386..16586)
                     /locus_tag="PWY43_000929"
     CDS             complement(16386..16586)
                     /locus_tag="PWY43_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL2135823.1"
                     /translation="MKSILKVVGAMVLMVAVSGCNNMTKTQRNTAIGAAIGGVAGHAI
                     GESTGATLGGAALGGVIGSQIK"
     gene            16811..17845
                     /locus_tag="PWY43_000930"
     CDS             16811..17845
                     /locus_tag="PWY43_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2135824.1"
                     /translation="MKLKKLSLALSTLLLGSAFSNSVLAEGRLTVYCSAANELCEQEV
                     QAFGKKYDVKVAFVRNGSGSTLAKIEAEKNNPQADVWYGGTLDPHSQAAEMGLLEPYK
                     SPNLEQIIEKFRDPAKLKGNYSSAVYMGILGYGVNLDRLKKLGIEKVPSTWEELLDPR
                     LAGEIQIADPQSSGTAYTAIATFVQLWGEEKAFDYFRKLHKNISQYTKSGITPARNTA
                     RGETTIGIGFLHDYAIEKKNGANIEMTAPTDGTGYELGGVSILKGARNLDNAKLFVDW
                     SLSKEAQELSWQKGNAFQVLTNTTAEASPYALDPKNLNLINYDFEKYGSSEERKRLID
                     KWVNEVKLAK"
     gene            17913..19949
                     /locus_tag="PWY43_000931"
     CDS             17913..19949
                     /locus_tag="PWY43_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2135825.1"
                     /translation="MQNFSFSRPLVWVFIGLLGFLLLPSKALDYGLFDSTSDELLAAM
                     GWSSINITWAWFLPLLVFLLPNSTACKTRAKLELVGLGVLFAFIFLSAIVLKISLGYA
                     VLPLIIAIMGLATQNLATLKVMQGDRFIIGSLLSIILLIFFFIVYPTIAIFVSMFYNG
                     SEFVPSQVLTILQQPYVVRVIGNSLSVAGTVGVLSTLFGLAFALYTTRIAKRSAFIGR
                     IFSILPIVTPPFVVGLGVTLMLGRSGYVTEFLVDYFGFANNWLYGFTGIVIAHTLALT
                     PMSFMILEGALKSIHPSVEEAAYTLRSNRYQAFFNIIFPLLKPALANSFLVVAIQSLA
                     DFSTPLVLGGSFDVIATQIYFYIAGSQLDYASASTLGAILLVFSLAIFVIQYLWIGNR
                     SYVTVSGKSYRGDVQDLPSGMKWLIICTLLFWGTFNLVLYGSIFYGSFTVNWGVDYTL
                     TLDNYIKLFGQGFSDGGFPSLIQTVIFAAAAAPITALFGLLIAYVTVRRDFKGKKTLE
                     FLTLLCFAVPGTVAGVSYILAFNDAPIYLTGTGMIIILSMVMRNMPVGMRAAIAGLRQ
                     LDKSLDEASLSLKAGSFKTMLFVVLPLLKPALLSALVTSFVRAMTTVSAIVFLVTADT
                     RVATSYILNRVEDGEYGIAIAYGSILIVVMMAIILIFDWIVGDTRIARSKAKKM"
     gene            20047..21096
                     /gene="fbpC"
                     /locus_tag="PWY43_000932"
     CDS             20047..21096
                     /gene="fbpC"
                     /locus_tag="PWY43_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDL2135826.1"
                     /translation="MNNDFLVLKNVTKSFGKVTVIDNLNLSIPQGKMVTLLGPSGCGK
                     TTVLRLVAGLENPTSGQIFIDGKDVTQSSIQHRDICIVFQSYALFPHMSIGDNVGYGL
                     KMQGVPKEKRLQRVKEALELVDLGGFEDRFVDQISGGQQQRVALARALILKPKVLLFD
                     EPLSNLDANLRRSMREKIRELQQRLNITSLYVTHDQSEAFAVSDEVIVMNKGKIMQKA
                     PAKELYLRPNSLFLANFMGESSIFEGTIAGNQININGYQFTLSNAEQFELPDGECLVG
                     IRPEAIHLAEQGNISQLCKIKNAVYMGNHWEIIAEWEGKDLLVNTKPENFYPHIKQAY
                     VHFIEQGVFLLKKEL"
BASE COUNT         6041 a         4752 c         4187 g         6259 t
ORIGIN      
        1 gaaagttagc taatacgatt caattaaaaa caaaatttat ttcatcaatg tcttaattat
       61 taaaaaatat ggtactaaat catcaaggca ttgcatggct ccctgaatat tctattagtg
      121 aagaattaca caataaaaaa gtaatcatac tagacaaaaa tgaattagtt attccaataa
      181 aaggttatat ttacagaatg aatacgagac taaataatgc agcagaaagg ttttggaata
      241 atttacaaaa tattcagttt ataaatgaag atatattaaa taaaccctaa cattctataa
      301 taagaaaaag cggtcgattc tctgccaaat tttgcaaatt ttagaagaaa acagaccgct
      361 tgtcagggtt ggaattagag taaccagctg ccaatcaatg cacaggcaaa accggttaaa
      421 ccgatggagg tttcaagcat tgtccaggtt ttgagggtgg ttttttcgtc catttcgagg
      481 aaacggctca tcagccagaa accggagtca ttgacgtggg aaagcacggt agcaccagag
      541 gcaatggcaa tcacgataaa gcagaggtcg aactggctta aatcggtggc tgctgcgaca
      601 gttggtgcaa ttaacgccgc tgtggtggtt aaggcaaccg ttgccgagcc ttgtgcaaca
      661 cggaaaacgg tggcaataat aaaggcagcc accacaatcg gcattccggt gtctttcatc
      721 atttccgata acacatcgcc aatcccgctg gcacgcaaca cgccaccaaa cattccgcca
      781 gcaccggtca ctaagacaat cgagcagatt gggccaaggg cattgttaca gattttttcg
      841 atttggtcga aactgcgatt atcccgcaac agcataatcg ccacaattaa ggtaatcagt
      901 aaagcaacag gggttttgcc gattaagcga aggctctgca cccacatttc actgccatca
      961 atcactttgg caaccgagag ggtattcaag ccggtatcaa acaaaatcag gataatcggc
     1021 agcaccaaca ccgtgagtac cttgccgaaa cttggcggat tttgcactgc catttcgttt
     1081 gccgataagc cgtttaaaaa ggctttcggt agatcaacgt gaatgcgttt gctgataaat
     1141 tggctgaaca gataagtgcc gatataccac gttgggattg cacaaaccaa gcccaccaag
     1201 actaacagcc ccatatttac accaagcaga tcgccggagg ccaccgggcc ggggtgtggc
     1261 ggtagaaaag cgtgcattac cgcaaacgca ccggcagacg gaaacgcata acgcaatacc
     1321 gagccaccga actgtttcgc cacactaaaa ataatcggca acatcaccac caagccggca
     1381 tcaaagaaaa tagggaagcc gaataacagc gaggcaacac ccaatgtaaa gggtgctttc
     1441 tctttaccga atttattgat aagcgtatcg gcaagcactt ttgcaccacc ggtgatttca
     1501 agcaagcgac caatcatcgc cccaagcccg accagtaacg ccactgaagc aagcgtgctg
     1561 ccaaagccgt ttaacaaagt tggcaggatt ttatccaccg gaataccggc agctaatgca
     1621 gtgagcaggc tcacaataat caatgccaca aaagcgtgga ctttaaattt cataatcagc
     1681 accagcaata aaatgatggc agcaaacata atgaacagta acataggatc tcctaaggtt
     1741 gaattgttat cggtaacata acccaaaagc agtttagagt aaacggttgc agggctttta
     1801 ttttaaaaat gtgatctact tcaagttttc tggaaatagc agatgatttt tcagcataac
     1861 ctgttacatt aacggctaag ttaacgttac cagtaacaca aggaaattca atggcaaacg
     1921 gcaaagcatt tattttgatg ggcgtgtcca gcaccggcaa aaccagcgtg ggaacggcag
     1981 tcgcacagcg gttgggaata aaattgattg atggggacga cttacaccct cgtgccaata
     2041 tactgaaaat ggcaagtggc acgccactca atgatgcaga tcgcacaccg tggttggagc
     2101 ggattaacga tgcagtgttc agttttgaac aaaaaagcga aaaagggatt atcgtctgct
     2161 cagcattgaa gaaaaagtac cgagaccaga tccgacaagg caatgccgat gtgacatttc
     2221 tattccttca cggctcattt gatctggtgc tggctcgaat gcaacaacgt aaagggcatt
     2281 ttatgaaacc ggaaatgctc caaagtcagt tcaatacact ggaaattcca caagcagacg
     2341 agccagatgt gatttttatt gatattgacg gcagttttga tgaggttgta gagcgttgca
     2401 taaaggctgc tgagccgttg atttaatcac aagctgttga ttttgcagat ttttttgcaa
     2461 atttaaccgc ttgcctttcc tacacacttt cccccaaatg aatgtgatag cccaagttaa
     2521 ttattgggct ttgttgcggt acgccgtgaa tgcggtcgag gagttcttgg gcggcaactc
     2581 tgcccatttc aaggcggggc gtcattacgc tggcaagctg tggcgtgagg gattgcccaa
     2641 catcgtggcc gtgaaagcct gcgattttaa tctgctgcgg cacattaatg cctaaccgtt
     2701 gacactcaaa taatgcaccg attgccaagt cgtcattagt gcaaaaaatg ccgtccagtt
     2761 gtggaatttg ctccaaagct tgttttagct ggcttgcgcc aagcgtgaaa gaagaatgct
     2821 ctgcagtagt aatgctgtgt gggcttaagc cgtgtttgag cattgcctgt tcgtagccct
     2881 gcattttcag ctgagagcgt ttatccattc tcgccccgaa atacaccacc tgtcggcagc
     2941 ctcgatgaat cattgtttct accatcgctt gagccgcaga aatattgtca aatccaaccg
     3001 cttgctgaat cccacattca ccactttcca ttatctctat taccggaata ttcgccacct
     3061 ccagcatttt caaggtgcgg ggcgagtggt gattttccga taaaataatg ccgtcaatat
     3121 tgtacgaaag cagcgactca atccgctgtt cttccttctg ctcgctgtaa ccatagtggg
     3181 caagcatagt ttggtagccg gcttggtcgg taatctgctc aatgccttta atcacatcag
     3241 caaacacttg gttggtaagc gagggaacca gcacgccaat cgctcgactt ttggcattag
     3301 agagaatatc cggtgcacgg tttgggatat agccaaacag ctcaatcgct tgtgcaatcc
     3361 gttcgccggt ttctttggca accgtgtttg gatctcgcaa aaaacggcta accgtcattt
     3421 tagtgatgcc gagatgagag gcgatgtctt gcaaagttgg gcgtttcggt ttactcattg
     3481 gaatagataa cgaaaaaatt taattttgct gattataact gaagataagg caataattgc
     3541 aaaatattcg gtgaaattaa ccgcttgtta ctactcttgc ctgaactctt tcggcgtttt
     3601 gccaaattct tttttaaaca cggcatagaa atattgtaaa gagggataac cacacacatt
     3661 ggcaatttct tgggtcgaaa tctcggtaaa tttgagcata tattttgctc ttgctaattt
     3721 ttcttcgtga atcacttggt gaatcgtctt attcatttcc tctttaaaac gttgttcgag
     3781 attagagcgg gaaactttga gatgatccag cacttgctct actttaatac cttgacatgc
     3841 tcgatgacga ataaagtgca tagcttgaat taccattggg tcttgtaaag atcggtaatc
     3901 ggtagaacgg cgctcctcaa cttttaccgg tggaattaaa atcggcttaa gagaaacggg
     3961 taaattattt aatttacgat acagtaattt cgcggcgtga taaccaattt gcgatgttcc
     4021 ctgtgtaact gaagaaagcg aaacccgaga gaggtattga atcagctctt cattatcaat
     4081 gccgatcacg caaagttgtt caggtacagc aatttttaaa tgctcgcagg tttgaagtag
     4141 atgtcttgcc cttgcatcag ttaccgcaat aataccagta tgaggtggta aagtttgcaa
     4201 ccactcgcct aatttagctt gtaattccac ccagttgtct gaatggaatt gatcgccaat
     4261 atagcaattc ggctgatgtt cgtatttttc catcaactga ataaaggcat tttggcgttc
     4321 tatcgcccag tgtttttccg tttctgccgg atagccgtaa aatgcaaatt gatctatacc
     4381 tttctgtttt agatgcaaaa atgcagcctc aattaaggcg taattatcgg tggcaacata
     4441 aggaagatta gggtaatagt cggagttttg gtaagaacca cctaccgcca caatcgggac
     4501 atcaatatct ttaagcaact cgaccgtttc taaatcgtca aaatcggcaa taataccgtc
     4561 aatcaacagg ctttcgatac tgtttttatg atagacaaaa tcgtcttcca caaaaatatc
     4621 ccaagtacat tgtgaggctt gaataaactg cccgatgcct tgcattactc ccctgtcata
     4681 aattttgtta gcattaaaaa gtaaggcaat tttataagat tgttctatca ttacttcctc
     4741 cagtacccac tttcaaaagt aactataaaa taaaattatt ttttgatcaa agcgaataaa
     4801 agaataaaac cccagtactt ctactggggt tttaagctca attttgatta agattattcc
     4861 gcagctgctt cagttgcaac atctgggcga tccactaact caatgtaagc cattggagca
     4921 ttatcacctg cacggaaacc acattttaag atgcgagtgt aaccacccgc acgttgtgca
     4981 aaacgtggac ctaattcatt gaataattta gcaactgtat caacgttgcg tgtacgggca
     5041 aaagctaaac ggcgatttgc aacgctgtct gctttagcta aagtaattaa cggctcaact
     5101 acacggcgta actctttcgc ttttggtaaa gttgtcttga tgatttcgtg ttcaacaagc
     5161 gcacttgcca tattacggaa catcgcttgg cgatggctgc tgttacggtt taattgacgt
     5221 ccactcttac gatggcgcat aacctattcc ttcttagaaa atctataccg aaaaactagt
     5281 cttctgcaat acttgcaggt ggccagtttt caaggcgcat accgagtgat aaaccacgag
     5341 aagcaagaac atccttaatc tctgtaagag attttttacc caagttcggg gttttaagaa
     5401 gttcaacttc tgtacgttgt actaaatcac cgatatagtg aattgtctct gctttcaaac
     5461 agttagcaga acgaactgtc agctctaggt catctactgg acgaagaaga ataggatcaa
     5521 attccggttt ttcttctttc acttcaggtt gacgaacatc acgcaaatca acgaatgcag
     5581 caagttgttc tgctaaaatc gttgcagcac gacggatagc ttcttcagga tctaacgttc
     5641 cgttagtttc catctcaata acgagtttat ctaagtctgt acgttgttca acacgagcag
     5701 cttcgacatt gtaagcaatg cgatctacag gtgaaaaacg tgcatctact aataaacgac
     5761 cgataggacg gtcatcgttt tgagtatgaa gacgtgctga tgcaggaaca taacctcgac
     5821 cacgttgaac acgaatgcgc atattaattg acgcattctt atctgttaaa tgacagatta
     5881 cgtgatcagg attcacaatt tctacatcgc catcgtgtgt gatgtcggct gcagttacag
     5941 ggccaattcc agatttattt agtgtcaaaa taatatcatc tttattttgc acttttaccg
     6001 ctagaccttt aaggtttaat aatacctcaa gaatatcttc ctgaacgcct tctttgctgc
     6061 tgtactcatg tagtacacca tcaatttcaa cttcagtaac tgcacaaccc ggcatagatg
     6121 aaagtaaaat gcggcgaagt gcgttaccta atgtatggcc aaaaccacgt tctaacggct
     6181 ctaatgtcac tttagcatga gttgaactaa cttgttcaat atttactaag tgcggcttta
     6241 aaaattctgt cacagaaccc tgcatttttt cctctctttg cggttaagct aactattatt
     6301 tagagtaaag ctcaacgatc agatgttcat taatatctgc tgatagatca gaacgctctg
     6361 gacgacgttt aaatacgcct tccattttag ttgcatcaac ttctaaccaa gttggttttt
     6421 cacgttgagt tgctaattct aatgatgctt taatacgtgc ttgttttttc gatttttcac
     6481 gaacagcaac aacatcatca acagaaactt ggaatgaagg aatgtttact acacgaccgt
     6541 ttactacgat tgatttatgg ctaacaagct gacgtgcttc tgcacgtgtt gctgcaaaac
     6601 ccatacgata aactacgtta tctaaacgac cttccaataa tactagtaag ttttcaccgg
     6661 tattaccttt taagcggttc gcttctgtat aataattacg gaattgacgc tctaagatac
     6721 cataaatacg gcgaactttt tgtttttcac gtaactgact accatagtca gataaacgtg
     6781 gcttgcgagc accatgttga ccaggcgcta catcaagtct atttctacat tttgattcaa
     6841 tcgcacgaac gcctgatttt aagaataaat cagtaccttc acgacggctt agcttgagct
     6901 taggacccaa atatcttgcc attttctttc tccaattctt ttaacgtcaa ttaaacacga
     6961 cgttttttcg gtggacgaca accgttatgt ggaatcggag tcacatcagt aatattcgtg
     7021 atacggaaac ctgctgcatt taatgcacgg attgttgact cacgacctgg acccggtcct
     7081 ttaaccataa cttccaaatt ctttaaacca aactctttaa ccatttctgc acaacgctct
     7141 gcagcaactt gagcagcgaa tggagtcgat ttacgagaac cacggaaacc tgaaccacct
     7201 gcagttgccc aagctagagc attaccttgg cggtcagtaa tagtaacgat tgtgttattg
     7261 aaagatgcgt ggatatgagc tacgccatct gcaatctgtt tttttacgcg cttacgtgcg
     7321 cgaactggtg ttttagccat tatttatcta ctccgactat tttttgatcg gtttgcgtgg
     7381 acctttacgg gtacgcgcat tagtcttagt acgttgacca cgtaccggta aactacgacg
     7441 atgacgtaaa ccacggtagc aacctaagtc taaaagacgt ttgatgctta aagttacttc
     7501 acgacgtaaa tccccttcga cagtaaattt accaacttct tcacgcagtt tttcaatctg
     7561 ctcttcagac aattctctga tctttacatc ttcggcaata cccgttgcag cacagatggc
     7621 ttttgcacga gttttaccga taccgtaaat tgcagttaat gcgattacag tgtgtttttg
     7681 atcaggaatg ttaatgcctg caatacgggc cactatgcac tcctattatt taagtttatt
     7741 gatatactga tcttgaaaag cccgtttcag gatactcaaa cagtatatca ggacgaatga
     7801 gctgagcagt ataactgctc agcgggttct ttgcaagaaa tatgcgtaaa ttaaccttga
     7861 cgttgtttgt gtttaggatc gctacaaatt acacgaacca caccttcacg tttgattact
     7921 ttacaattac ggcacattct ttttactgaa gcacgaactt tcattgctta tcctttatta
     7981 acggcctatt aatttagacc ttttaagtta gctttcttca acacagaatc atattgttga
     8041 gacattaaat gactctgaac ctgtgcgatg aaatccataa taacaaccac tacaatcaat
     8101 aatgatgtac cacctaattg gaatggcacc ttccataacg acgtaatgat gtaaggaacc
     8161 aaacaaacaa aagcaatata taacgcacca attaaggtta aacgtgtcat tactttatcg
     8221 atataacgag atgtttgttc gcctggtcgg taacctggta caaacgcacc tgatttctta
     8281 agattatccg ctgtatcacg aggattatac tgcattcctg tataaaagaa agcaaagaaa
     8341 atcactgcca ttgtgaataa tacaacatac agtggttgac ctggctgtaa tagttgtgaa
     8401 aggttaaata accactcaaa tccttcgctt tgaccaaacc attgagtaat acttgccggg
     8461 aataaaataa tacttgaagc aaaaattgcc ggtattaccc ctgccatatt cacttttaat
     8521 ggtaagtgag aagttcttgc aggcgccatc attcgacctt gttggcgact tgcatagttt
     8581 acaactattc ttctttgtcc acgttcaaca aaaacaacaa aataagttac tgcaaatgca
     8641 attattgcaa ctagtagtaa gacgataaat gataactcac cttgacgggc ttgttcaatt
     8701 gtctgcccta tcgttgctgg aagatctgca acaatacctg caaaaataat caatgagata
     8761 ccgtttccga taccacgctc agtgatttgt tcgcctagcc acattaggaa cattgttccc
     8821 gtcactaagc ttactacaga agttacatag aataatatac ttggattagg aactaatcca
     8881 tgtaccatat tcggtagggc tagtgaaata ccaatagatt gaataaatgc taataacagt
     8941 gtgccatagc gtgtatattt actgattttc cgacgacctg cttcaccttc tttctttaat
     9001 tcagctagag gtgggtgaat cgctgtcaat aattggataa taattgacgc tgaaatataa
     9061 ggcataatac ctagtgcaaa tatagatgca cggcttaaag caccaccaga gaacatatta
     9121 aacatatcaa tgatggtgcc ttgctgttgt cgaattaatt cggataatac agaagcatca
     9181 atgccaggaa caggtataaa agaaccgata cggaaaacga ttaacgcacc taaaacaaac
     9241 aatagtcttg atttaagctc gccagtccct ttttgtgtac taccttggta ccctggttgc
     9301 tttgccatct ttattcctcg atagaaccgc cagcagcttc aatagcagct ttagcgcctt
     9361 tagttacacg taagccttta acaactactg ctctttcaat tttgcctgct aaaattactt
     9421 tagcagataa aatatcttta gtaatgatgt ttgctgcttt taatgtctct aaagttactt
     9481 cgttgccatc tactttagct aagtcgttta aacgaatttc agccacatgg aatgatttta
     9541 atgaagtaaa accaaatttt ggcaaacgac gatataaagg catttgacca ccctcgaaac
     9601 cacgacgaac accgccgcct gtacgagatt tttgaccttt atgaccgcgc ccaccagttt
     9661 tacctaaacc agaaccaata ccacgaccta gacgttttgc actgtgctta gcaccttcag
     9721 ctggagaaag agaatttaaa cgcattctat tactcctcta ctttaaccat ataagacact
     9781 tgattgatca taccacgcac tgctggtgta tcttcaagtt ctacagtatg acgaatatga
     9841 cgaagaccta agccttttaa cgttgcttta tgtttcggta aacgagcgat agagctacga
     9901 gtttgagtta ctttaatttt tgccatatcc aattacccca aaatttcttc aactgttttg
     9961 ccacgttttg cagcaaccat ttcaggtgat ttcatgttct caagtgcatc gattgttgca
    10021 cgaacaacgt taattgggtt agttgaacca tacgctttag aaagaacgtt acgaacaccc
    10081 gcaacttcta atactgcacg cattgcacca ccagcgatga tacctgtacc ttcacttgct
    10141 ggttgcataa atacgcgtga acctgtatgt gaacctttaa ctggatgttg taatgtacct
    10201 tcatttaaag ctacattaat catattgcga cgagcttttt ccatcgcttt ttggattgct
    10261 gctggaactt cacgtgcttt accgtaacca aaacctacac gaccgttacc atcgcccact
    10321 actgttaaag cagtgaaact cataatacga ccacctttta cggtttttga aacacggtta
    10381 accgcgatta gcttttcctg cagttcacca gcttgttttt cgatgtttga catctcaaat
    10441 tcctctatta gaactgtaga ccagcttcac gagcagcatc tgctaatgat tgcacacgac
    10501 cgtggtattt aaaaccagaa cggtcaaaag caaccgcttt aatacctttg gcaattgcac
    10561 gctcagcaac gattttaccc actactgctg cggcttcttt attgccggta tatttaactt
    10621 gctctttaat tactttttca acagttgaag ctgctgctag tacttctgag ccattaggtg
    10681 caatcacctg cgcatagata tggcgaggtg tgcgatgcac aactaaacgt gttgcacctt
    10741 gctctctcat taaatgacgt gcacggcttg cacgacggat acgagctact ttcttatcca
    10801 tagtgttacc ttactttact ttttcttcgc ttcttttgta cgcactactt catcagagta
    10861 acgtacacct ttacctttat aaggttcagg tttgcgatat gcacgaatat ctgctgccac
    10921 ttgaccaatt aactgtttgt cagcactttt cagaataatt tctgtttgtg atggacattc
    10981 accagttacg cctgctggca acgtgtgttc tactgggtga gagaaaccta aacttaaagc
    11041 aacagcattg cctttcattt gtgctctata accaacaccc actaattgca atttcttagt
    11101 aaagccttca gtaaccccga ttaccatatt atttactagt gcacgagcag tacctgcttg
    11161 agcatctgca ttagcgatac cttcacgtgg agcgaaagtt aatgcatctg cttcttgttt
    11221 tacttcaact gcattgtgaa tttcacgaga taactcgccg tttttacctt taactgttaa
    11281 tagctgaccg ttaagtttta cctcaacacc ggcaggaata ttaacaggtg cttttgcaac
    11341 acgagacatt tttcctacct ctctattatg ctacgtaaca gataatttca ccgccgaggc
    11401 ccgcttgacg cgcagcacgg tcggtcatta cacctttaga tgtagaaaca actgcgatac
    11461 ctaaaccacc cattactttt ggtaattcgt ctttacgttt ataaatacgc agacccggac
    11521 ggctcacacg ttggatactt tctacaaccg gtttgttttg gaaatatttt aaagtaattt
    11581 ccagttcagg tttaacacct tcaacaactt taacgctttc gatataacct tcttcagcta
    11641 aaacatttgc aatagcaact tttaacttag atgaaggcat actgattgca actttgttcg
    11701 cagcttgacc gttacgaata cgggtcagca tatctgcgat tggatcttgc atgctcatgt
    11761 atttactccc atgattccaa attaaaagag gttattacca gcttgccttc ttaagacccg
    11821 gaatttcacc gcgcatagca gcttcacgaa ccttaatacg gcttaaacca aacttacgaa
    11881 gtacgccatg tggacgacca gtttggcggc aacgattacg ctgacggact gggctagaat
    11941 cacgaggaag tgtttgtaat tttaacactg ctgcccaacg gtcttcatca gatgcattca
    12001 catcagagat gattttcttt aattcttcac gttttgcgta gaatttatca gctaatttag
    12061 cacgttttac atcgcgtgcg atcattgatt gttttgccac gataacctac cttatttacg
    12121 gaatgggaaa ttgaaagcag ctaataaagc ttgaccttct tcatcagttt tcgctgaggt
    12181 ggtgatagta atatctaaac cacgaacacg atctacttta tcgtagtcga tttcagggaa
    12241 aatgatttgt tcacgaacac ccatactgta attaccacga ccatcgaatg acttagcgtt
    12301 taaaccgcgg aagtcacgaa tacgtggaac agcgatagta atcaatcttt caaagaattc
    12361 ccacatacgt tcaccacgta gggttacttt gcagccgatt ggataccctt gacggatttt
    12421 aaagcctgca acagatttgc gagctttagt tactaaaggt ttttgaccgc tgattgctgc
    12481 taaatccgct actgcgttat ctaacagttt tttgtcggtc aatgcttcac ccacacccat
    12541 gttaagggta atcttttcga ttcgtgggac ttgcatgaca gatgagtagt tgaattttgc
    12601 ttttaattca ttaactactg tatctctgta gtaatcatgc agtttcgcca tcacattact
    12661 ccagttaatt aaattgtttt ctcgctagat ttgaagaaac gcactttttt gccatcttcg
    12721 aatctaaatc ctacacggtc agctttgttg gtttcaaggt tgaagatcgc aacgtttgaa
    12781 acatcgattg cagcttcttt cttcactaaa ccaccagctt tgcctaatgc aggaactggt
    12841 ttttcgtgtt tagtgatgat gttgataccc tcaacaaaaa ctttaccatt tggtaacact
    12901 ttagttacct taccacgttt gcccttatct ttaccggtaa gaacaattac ttcatcgttt
    12961 tgacggattt ttgcagccat tacattctcc ttatagtact tctggagcta aagagatgat
    13021 tttcataaac ttctcagaac gaagttcacg agtcaccggt ccaaaaatac gagtaccgat
    13081 tggttgctca gtgttattgt ttaaaattac acaagcattg ccatcgaaac gaataactgc
    13141 gccatctggg cgacgaacac ccttcttggt gcgcacaaca actgctttta acacatcacc
    13201 ttttttcact ttaccgcgtg gaattgcttc ttttacagta actttaatga tatcgccaat
    13261 agcagcgtaa cgacggtgcg atccacctag aaccttgata cacattacgc tacgagcccc
    13321 tgagttatca gcaacatcca gcatagtctg ttcttggatc atattagtgc tccgttaaaa
    13381 taaaaaaaca ccctttcggg acgaacctat ttatacaaat ataaataggg tgcaaaatat
    13441 actacaaaaa ccactagtta atcaagtgaa tttttaagct aatttaatag tttaagtaag
    13501 cttatttata aattgtttca actatcaata aactgtccta taactcatat aaaaacccca
    13561 agatatcttg gggtttaact aacattactc tacagtcacc gcttttgcaa ggttacgagg
    13621 ttggtcaacg tccgttcctt tgattaaggc aatgtgatat gaaagtagtt gtaacggcac
    13681 ggtgtagaaa atcggtgcgg ttacttcatc aactttcggc attacaatcg ttttgaagcc
    13741 ttctgcttct tcaaaacctg catcgtgatc ggcaaacaca tataactgac cgcctcttgc
    13801 acgcacttct tcaatattgg atttcacttt ctcaagcaag tcattttccg gtgctaccac
    13861 cactaccggc atatcgctat caattaatgc taacgggccg tgttttaact cgcctgcggc
    13921 ataagcttct gcgtgaatat atgaaatctc ttttaatttc aatgcagatt ccatcgcaat
    13981 cgggtaatat tctccacgac ctaagaacaa tgtgtggtgt ttatccgcaa aatcttccga
    14041 taatttttcg atttgtttat cgaacactaa cgcactttca atttgtgctg gtaagcgttg
    14101 taaagattgc acgatatggt gttcttgctc ttcactcatt ctgccttgta agcgtccaat
    14161 cgccacattt aataacaata agcaagttaa ttgagtagtg aatgctttgg ttgatgctac
    14221 accaatttca acaccagctt tggttaagaa tgcaaaatcg gattcacgca ctaatgaaga
    14281 acttgccaca ttacacaccg tcattgccgc catatagcca gattctttcg ctaaacgcag
    14341 tgcagctaac gtatctgcgg tttcacctga ttgtgaaagc gtgattaata agctgttcgg
    14401 gcgagtaacg aatttacggt aacggaattc ggaggcaatt tccacatcac agctcacgcc
    14461 tgcaatggat tcaaaccaat aacgtgaaac cataccagca ttgtaagagg ttccacaagc
    14521 cacaatttgc acgtgttcta ctttcgataa aatctctgcc gcattcggtg caatggctga
    14581 aatatctact ttaccgtctt tgatacgacc atctaaggta ttcataatcg caaccggctg
    14641 ctcaaaaatc tctttttgca tatagtgacg atattgtcct ttatctgccg cgtctgcctc
    14701 aaaattgcct tcgtgaattt cacgctctac cttgttgcct tcacggtcgt aaatatccac
    14761 tgtgcgacgg gtaatttcgg ctacatcacc ttcttctaga taagcaaaac ggcgagttac
    14821 gcttaataat gcaagtgggt ctgaagcaag gaagttctcg cctacaccgt aaccaatcac
    14881 taacgggctg cctgagcgag ccacaattaa atgctccggc tcttcttcat ttaataccac
    14941 tgtgccgtaa gcaccacgca gttgcaccac ggttttttgt accgcttcta ataatgaaga
    15001 agcagaacgc aattcccatt caactaaatg agcaatgact tcggtatcgg tttgagattg
    15061 gaacacataa ccacgctctt gtaacactat ttttaactct tcgtagtttt cgataatccc
    15121 gttatgaact actgcaattt tgccgctgcg gtgtgggtga gcattagttt ctgacggctc
    15181 gccgtgcgtt gcccagcgag tgtgtgcgat cccagtgccg cctaatagtg gcttagtgtc
    15241 taacgcctca tctaaggctt ggactttacc gacacggcgt acaatttgca ttgttttatc
    15301 atctgcattt aatactgcta caccagcaga gtcatatcca cggtattcta agcgatgtaa
    15361 accatcaacc aagatttctg cgatatcacg ttgtgctact gcacctacaa ttccacacat
    15421 aaatttgctt tcctatgttt aagtttaaag attcatttga gctactgcta ttttgcaata
    15481 accacttcga cacccatttt ttcgatttgt tctttcgttt caaaacttaa ttgactgtcg
    15541 gtaataattt tggtaatatt ttgccattcc aattcaatat tcggcatttt ccgaccgatt
    15601 ttttgcgatt caattaacac aatcacttca cggctcactt ctgccatcac ctgacttaag
    15661 ccaactaatt cattaaaggt agtcgtgcct cgagccaaat ctaaaccgtc cgcaccaata
    15721 aaaagctgat cgaaatcata tgaacgcaat acctgttctg ctactttgcc ttgaaaggac
    15781 tcagaacggc tatcccacgt accaccggtc atcaacactg ttggttcgca ttcaagggaa
    15841 gtcaattccg ccgctaaatt cagcgaattt gtcatcacca ccaagccggt ttggttcaat
    15901 gcttttacca aagctccggt cgtgctgccg ctgtcaataa tgatgcgatt atgatcacga
    15961 atacaatcac gtgcggcttg agcaatcatt ttcttttgaa tcgaaagttg ctcaataaac
    16021 gcttcttcca tcatttccga tggaattttt accgccccac catacttacg caataaaaaa
    16081 ccactttctt ccaaagcggt taaatctttt ctaatcgtaa cttcagacgt ctcaaagaaa
    16141 tgagcgagtt catctacact cacttcattt ttctcttgta ccatttgaac aattaaatgg
    16201 cggcgttgtt gcgtgttacg ttttgacatt gtaatcttca cttaaatttc gtttcaaaac
    16261 ttttgcatta taaagaaagc tcgcgaaaag aaaagcagaa aatgcaaaat ttacaaaatc
    16321 tatacaaaat aaaaccgctt gtaatattga tttacaagcg gttgatttct tgaaattttt
    16381 tgcaattatt tgatttggct accaatcacg ccaccaagtg cggcaccacc taaagttgca
    16441 ccggtagatt caccaatcgc gtgaccagcc acaccgccaa ttgctgcacc gattgcagta
    16501 ttacgttgcg tttttgtcat attgttacag ccagacacag caaccattaa taccattgca
    16561 ccaacaactt tcaaaataga tttcataata atccctcata attaatcaat ttcaaataag
    16621 ccaatattct tggctatgct ataagattta atttatttag aaaggttcaa ctactaccac
    16681 ctatgaattg caaatttttt cacaatttag gccgcttgtt gattgaactg tgagctcgct
    16741 cacagaattt catgacattt cttgctatat tgtgtagcaa tctatgcgat aactcatcaa
    16801 ggaaataaaa atgaaactga aaaaattatc tttagcactc tcgactttat tactcggctc
    16861 agccttttcc aactctgtat tagcagaagg tcgcttaacc gtttattgta gtgcggccaa
    16921 cgaactttgt gagcaagaag tgcaagcatt cggcaagaaa tacgatgtta aagtggcttt
    16981 tgtgcgtaac ggttcaggca gcacactggc gaaaattgag gctgagaaaa acaatcccca
    17041 agccgatgta tggtatggtg gcacgctaga cccacattca caagcggcag aaatgggctt
    17101 gttagagccg tataaatcgc caaacctcga acaaattatc gaaaaattcc gtgatccggc
    17161 aaaactaaaa ggcaactact cctccgctgt gtatatgggg attttaggtt acggcgtgaa
    17221 tttagaccgt ttgaaaaaac tcggcattga aaaagtacct agcacttggg aagaattgtt
    17281 agaccctcgc ctagccggtg aaatccaaat tgccgatccg caaagctccg gcacagctta
    17341 tacagcaatt gcgactttcg tgcaactttg gggcgaagag aaagccttcg actatttccg
    17401 caaattgcat aaaaacattt cgcaatatac caaatccggc atcacaccgg ctcgcaacac
    17461 tgcccgtggt gaaaccacca tcggtatcgg tttcttacac gactatgcta tcgagaagaa
    17521 aaacggtgct aatattgaaa tgaccgcccc aacagacggt acaggctacg aattaggtgg
    17581 cgtgagtatc ctcaaaggag caagaaacct tgataatgcc aaactgttcg tggattggtc
    17641 gctttctaaa gaggctcaag aactctcttg gcaaaaaggc aatgcgttcc aagtattaac
    17701 caacaccacc gccgaagcct cgccttacgc cctcgatccg aaaaacttaa acttaatcaa
    17761 ttatgatttt gagaaatatg gctcaagcga agagcgtaaa cgcttgatcg ataaatgggt
    17821 aaatgaagta aaattagcga aatagggttg atctttccac tgaggggata agtgattatc
    17881 ccctctctca ttataaaaat ctgtgaggtg ttatgcagaa tttttccttt tcccgaccgc
    17941 ttgtttgggt gtttatcggg ttactcggct ttctcttatt accgtccaaa gccttagatt
    18001 acgggctgtt tgactcaacc tctgacgaac tgctcgccgc aatgggttgg tcgagtatca
    18061 acattacttg ggcgtggttt ttaccgcttt tggtattttt attgccaaat tcgaccgctt
    18121 gcaaaaccag agccaagctg gaattagtcg ggttgggcgt actattcgcc tttattttcc
    18181 tgtcggcgat tgtgctgaaa atcagtcttg gttatgcggt gttgccgttg attattgcca
    18241 ttatggggct tgcaacccag aatttagcca cgcttaaagt aatgcagggt gaccgtttta
    18301 ttatcggctc gttactgagc attattctgc tgattttctt ttttattgta tatccgacca
    18361 tcgccatttt tgtgtcgatg ttctacaacg gctcggaatt tgtgccaagc caagtgctaa
    18421 cgattttgca acaaccttat gtcgttcggg tcattggcaa ctcgctctcg gttgccggca
    18481 cagtcggcgt tctctccacc ctattcggct tggcgtttgc cctctacacc acccgaattg
    18541 ccaaacgctc agcgtttatc gggcggattt tctcgatttt gcccatcgtc acaccaccgt
    18601 ttgtagtcgg tttaggcgta accctaatgc ttgggcgttc cggttatgtg actgaatttc
    18661 tagtggacta tttcggcttt gccaacaact ggctgtacgg tttcaccggt attgtgatcg
    18721 cccataccct cgctctcacg ccaatgtcgt ttatgatttt ggaaggggca ttgaaatcca
    18781 ttcacccatc ggtggaagag gcggcttaca ccctgcgctc caatcgctat caagcctttt
    18841 ttaacatcat tttcccattg ttaaaaccgg cgttggcaaa ctcctttttg gtggtggcga
    18901 tccaatccct tgccgatttc agtacgccgt tggtgttggg cggcagtttt gatgtgattg
    18961 caacccaaat ctacttctac atcgccggct cccaactgga ttacgcctcc gccagcacac
    19021 tcggggcaat tttgctggta ttctccctag ccattttcgt gatccaatac ctctggatcg
    19081 gcaaccgctc ttatgtgacg gtgtcgggca aatcctatcg gggcgatgtg caagacttac
    19141 caagcggtat gaaatggctg attatttgca ctttattgtt ctggggaacg ttcaatttag
    19201 tgctatatgg cagtattttc tacggtagtt tcaccgtgaa ctggggcgtg gattatacct
    19261 taacccttga taactatatc aagctgttcg ggcaagggtt tagcgatggt ggtttccctt
    19321 cgttaattca aaccgtgatt ttcgctgctg ccgccgctcc aattaccgcc ctatttggct
    19381 tgctgatcgc ctatgtgacg gttcggcgtg atttcaaagg caagaaaacc ctagagtttt
    19441 taaccctgct ctgctttgcc gtgccgggaa cggtggcagg cgtctcttac attcttgcct
    19501 ttaacgatgc cccgatttac ttaaccggca ccggtatgat cattattctc tcaatggtaa
    19561 tgcggaatat gccggtgggt atgcgggcgg cgattgccgg cttgaggcag ttagataaat
    19621 cactggacga agcctcgctc tcgctgaaag ccggttcgtt caaaacaatg ctgtttgtgg
    19681 tgttaccgct gctcaaaccg gctctgctct ccgccctcgt caccagcttt gtgcgagcaa
    19741 tgaccaccgt gagtgcgatt gtgttcttag tcactgccga cacccgtgtg gcaacctcct
    19801 acattcttaa ccgtgtggaa gacggcgaat atggcattgc gattgcctac ggctctattt
    19861 taattgtggt gatgatggcg attatcctga tttttgactg gattgtcggc gacacccgta
    19921 tcgcccgttc taaggcgaaa aaaatgtaaa aagacatcta ctctctccct ttatgggaga
    19981 gagacagcaa aatacttcgt tcagaagtat ttttcagaga gagggatata attaaaagga
    20041 caatatatga acaacgattt cttagtttta aaaaacgtca ccaaatcttt tggcaaggta
    20101 acggtcattg acaacctcaa cctcagcatt ccgcaaggca aaatggtgac cttacttggg
    20161 ccgtctggtt gcggaaaaac cacggtgcta cgtttggtgg ctgggctgga aaacccaact
    20221 tcggggcaga tttttattga tggcaaagat gtgacccaaa gttcgatcca gcaccgtgat
    20281 atttgcattg tgttccagtc ttacgctctg tttccgcata tgtcgattgg cgataatgtc
    20341 ggctacggct tgaaaatgca gggcgtaccg aaagaaaaac ggctgcaacg agtgaaagag
    20401 gcgttggaat tggtggattt aggcggtttt gaagatcgct ttgtcgatca aatctccggc
    20461 ggacagcaac aacgtgtcgc cctcgcccgt gccttaattc tgaaaccgaa agtgctactg
    20521 ttcgatgaac cgctgagtaa ccttgacgct aacttacgcc gttcaatgcg ggagaaaatc
    20581 cgtgaattac agcaacgttt aaacattact tccctttatg tgactcacga tcagtccgaa
    20641 gcctttgcgg tttcagatga agtaattgtg atgaacaagg gaaaaattat gcaaaaagct
    20701 ccagccaaag agttatacct tcgccctaat tctttattct tagcaaattt tatgggtgaa
    20761 agcagcattt ttgaaggcac gatagctggc aaccaaatta atataaatgg atatcaattt
    20821 acgttatcaa atgctgaaca atttgagtta cccgatgggg aatgcttagt tggcattcgt
    20881 ccagaagcta ttcatttagc tgaacaagga aatataagtc aactctgtaa aattaaaaat
    20941 gcagtttata tgggaaatca ttgggaaatt atagctgaat gggaagggaa agatttatta
    21001 gtcaacacta aacctgaaaa tttttaccct catatcaaac aggcttatgt acattttatc
    21061 gaacaagggg tttttctttt aaagaaagag ctataacgaa tttatactaa attaagagga
    21121 aggtagttat aactatttag caattttgag aaaaataggc cgcatgcaaa atctcttgat
    21181 gacaagcggt cttttcttgc cgattttttg caaatgcaat gatatggctg gcttaatcg
//
LOCUS       DANKBV010000032        21225 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.032, whole genome shotgun sequence.
ACCESSION   DANKBV010000032 DANKBV010000000
VERSION     DANKBV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21225
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.032"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            210..1508
                     /gene="tig"
                     /locus_tag="PWY43_000933"
     CDS             210..1508
                     /gene="tig"
                     /locus_tag="PWY43_000933"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL2135827.1"
                     /translation="MSFSIETLEGLKRRVTINVPADKIEAAYKEQLKGYAKNARVDGF
                     RKGKVPHSIIEQRFGIAARQDVLSDEMQRAFFEAVIAEKINLAGRPTFTPNNYQPGQD
                     FSFTAEFEVFPEVELKGLENIEVEKPVVEIADADLDKMVDVLRKQQATWSESQEAAKA
                     ESRVTIDFVGSVDGEEFEGGKASDFVLFMGQGRMIPGFEDGIVGRKAGEQFDIDVTFP
                     EEYHAENLKGKAAKFAITLKKVEEMVLPELTEEFVKKFGNAKSVEELRAEIKKNMQRE
                     LKNAVTSRVKTQVINGLIAQNDVEVPAAAVEEEINVLRQQAVQRFGGKPEMAAQLPAE
                     LFEAEAKRRVQVGLLLSSIIGSNELKVDEKRVEEMIADIASAYEQPAEVVAHYAKNRQ
                     LTENIRNVVLEEQAVDAVLAKAKVTEKTMSFDEVMSQQAQ"
     gene            1624..2076
                     /locus_tag="PWY43_000934"
     CDS             1624..2076
                     /locus_tag="PWY43_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2135828.1"
                     /translation="MEQLTFAQQLEQFFANHTLMVFAWVALLIAVIFSFYKSATQKYK
                     IVDNAQATQLINKEEAVVVDLRSDEEFRAGHIIDSIHVLPSDIKAAKINQIDKYKARP
                     VIVVDTNGFTSGGVAADLAKQGFSRVYVLKEGIGGWRAASLPTIKKHK"
     gene            2097..2597
                     /gene="secB"
                     /locus_tag="PWY43_000935"
     CDS             2097..2597
                     /gene="secB"
                     /locus_tag="PWY43_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDL2135829.1"
                     /translation="MSEENQVASTEEATQTPFELQIQRIYVKDVSFEAPNLPTIFQQE
                     WKPQLGFELDTETKEIGEDLYEVTLHITVQTTLEDSNDVAFICEVKQAGVFTIKGLEG
                     IQLAHCLASQCPNVLYPYARELISSLVNRGTFPALNLSPVNFDALFMDYLQRQEESAE
                     EPALAN"
     gene            2704..3714
                     /gene="gpsA"
                     /locus_tag="PWY43_000936"
     CDS             2704..3714
                     /gene="gpsA"
                     /locus_tag="PWY43_000936"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2135830.1"
                     /translation="MSEIYSAPITVLGAGSYGTALAIALSRNGHKTYLWGHNPDKMAV
                     LASERMNQDFLPDIVFPEALEIVADLATAVQKSKDLLIVVPSHVFSEVLAQIKPFLSQ
                     DHRIMWATKGLERDTGRLLQTVAKEILGEIYPLAVLSGPTFAKELAAGLPTAISLAST
                     DPVFADQMQKRIHCSKAFRVYLNNDIIGVQLGGAIKNVIAIGAGISDGMGFGANARTA
                     LITRGLAEISRLGVSLGADPKTFTGMAGLGDLVLTCTDNQSRNRRFGLALGQGKSAEV
                     AIAEIGQVVEGFYNTKEAYLLAQTQGVEMPIVEQIYQMLFCGKQASDVVKTLLGRERK
                     GE"
     gene            3730..4545
                     /gene="cysE"
                     /locus_tag="PWY43_000937"
     CDS             3730..4545
                     /gene="cysE"
                     /locus_tag="PWY43_000937"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL2135831.1"
                     /translation="MQQSEINQIWQAIRDEAKELANCEPMLASFFHATILKHHNLGDA
                     LSYILANKLENPIMPAIALKEIIEEAYRAEPQIIASAACDINAVRTRDPAVDKWSTPL
                     LYLKGFHALQSYRVTHYLWNQGRKALAVYLQNEISVAFDVDIHPAAKVGCGIMFDHAT
                     GIVVGETSVIENDVSILQGVTLGGTGKENGDRHPKIREGVMIGAGAKILGNIEIGRYS
                     KIGANSVVLQPVPDHATAAGVPARIIGKSSEQKPAFDMNQYFEDEQGLFGDGI"
     gene            4594..6771
                     /locus_tag="PWY43_000938"
     CDS             4594..6771
                     /locus_tag="PWY43_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S6"
                     /protein_id="HDL2135832.1"
                     /translation="MQLTFIQMFCPLLSGLTIFTICYVFPIVVIECLFCHQLMLEFAK
                     FYFLDDKYMKPASFKLSLISAFVGLSLAQLSYSSTVRGDIDYQYFRDLAENKGKFYVG
                     AKNVEIFNKQGDKIGIALENIPMIDFSVITRGGGYATLTKPQYIVSVEHNRGYRTVQF
                     GDSGSENPDNHHFNYQLVSRNDYDPKGSKAIQGFTHNDYQTPRLHKLVTETAPAEQSQ
                     EKNYDLYRDASIFPMFIRAGSGTQYILAEKNAVKEKLADPYAYLIGGTTLKLTSNNAN
                     TFVFKGNVFDDSYSPLSSHPQQGDSGKTFTWTALGNSSRITGATAELVVDLADTSLPD
                     PTTGKPHKPSLHHGKTLKISGDGNTLNLANNINQGAGALYFSGNAVVTGANEMTTWLG
                     AGISVDKNKNVEWQVHNPVGDRLSKIGEGTLTVSGKGKNLGSISVGDGTVILNQQAGE
                     NGEKSAFSEVGIVSGRPTVILNSADQVDPNSIYFGYRGGRLDLNGNSLTFNRIQNVDD
                     GARIVNNNAGTAANISLVGQVFTANDVRTINFGEGYNADLFRSQGAYFVLENNAWKFI
                     SWNHDDAKKYVVEKKNKALENQLYAYNGYFGESDSSRENGKLNIHFKPINAGGKLLLT
                     GGTTLDGILSAENDAEIILSGRPIPYARQVLDENKKTINKEIIKEGEWINRTFSATTF
                     QAKDNGKIEVSRNVVEVNGNFNLSDNATAQVGFTQGKSQSCVR"
     gene            6784..7215
                     /locus_tag="PWY43_000939"
     CDS             6784..7215
                     /locus_tag="PWY43_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopropylmalate isomerase"
                     /protein_id="HDL2135833.1"
                     /translation="MASCNLSVLSDKDLHSWQRTTVNSDIKLTQNARFELGSKADLIG
                     TIESNGDSQINLRNGSSWVMTGNSNVNKLNVDNATITLDNNVGEPNTLNINSLSGSGV
                     INFITYFAQTISDLINVEHASGAFKAKISQIGTPTTQRGVL"
     gene            7212..7364
                     /locus_tag="PWY43_000940"
     CDS             7212..7364
                     /locus_tag="PWY43_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL2135834.1"
                     /translation="MIFYKYINLKKASHLNFKVAKYIDEQGKSRKMYLMTQDGFTLLV
                     MGFTHK"
     gene            7361..7600
                     /locus_tag="PWY43_000941"
     CDS             7361..7600
                     /locus_tag="PWY43_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135835.1"
                     /translation="MNRTFALNKIAVERCKQDFPDCYHFKKLMAEECTELADRLKMGL
                     VLMAELKTTSTLTSDQNRHFKAFRNGAKYLIKQLE"
     gene            complement(7662..8849)
                     /locus_tag="PWY43_000942"
     CDS             complement(7662..8849)
                     /locus_tag="PWY43_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2135836.1"
                     /translation="MARKITPLNDTQIRKAKPEDSPLRDGNGLLLVITSNSKLWRFRY
                     ERPFTKKRNDLSIGAYPDISLAQAREIREEYRALLAQNIDPQTHRQQQEQAKANEISN
                     TYENVAWAWLEHRKTKKNFSDNYQKDVIALIKNNLLPHFGRMPITQITAPMAIKAFKQ
                     YQDEGKLEKLKRAIQKHNEIMMFAVHREMIPFNPTANIAKEFDSPTVEHFKTIKPEDL
                     GEFLYTLQNAQIHLQTRYLILWQLLTMTRPNEAAKAKWADIDEKAKLWTIPAENMKRG
                     IEHKITLSRQAIALLREIKKLSGGKTYLFPSVKNPQTHVNTQTANGAIKRMGYKGKLV
                     AHGLRAIASTYLNEKGYNSDLIEVALSHLNSDRIKAAYDRGERLEQRFKLLQVWADFP
                     PLV"
     gene            complement(9047..9413)
                     /gene="ssrA"
                     /locus_tag="PWY43_000943"
     tmRNA           complement(9047..9413)
                     /gene="ssrA"
                     /locus_tag="PWY43_000943"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            9579..10538
                     /gene="rpoH"
                     /locus_tag="PWY43_000944"
     CDS             9579..10538
                     /gene="rpoH"
                     /locus_tag="PWY43_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDL2135837.1"
                     /translation="MKKFNENEIEDAEIIDAEPIDVDEIDEEETSVEAMHPALVSGSM
                     PVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDVEAAKQLILSHMRFVIHIARG
                     YLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVK
                     VATTKAQRKLFFNLRKNKNRLAWFNEEEIKKVADDLGVSVEEVREMESRMTGQDLGFD
                     LPAGDDDEDSYAPSMYIEDSSSNFADDLEDEQHNGQATAQLAYALATLDERSQDIIKT
                     RWLDDNKATLQDLADKYNISAERVRQLENAALKKIKEAISLDG"
     gene            10679..10987
                     /locus_tag="PWY43_000945"
     CDS             10679..10987
                     /locus_tag="PWY43_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HDL2135838.1"
                     /translation="MINITDKQIDPTFYQRADGFINVANAHLKNIAPNQVSNAMLFGC
                     ARFNAYVAASKAEYKQQLADSREEVIQYFVEQYKEMLTANLDEYIQNFERYIEGKKAD
                     "
     gene            complement(11005..11307)
                     /locus_tag="PWY43_000946"
     CDS             complement(11005..11307)
                     /locus_tag="PWY43_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL2135839.1"
                     /translation="MNTYNNTLNNKNLEKKQLLMAIQAIPASQDFIWDGKDDDERPVT
                     PSEFSIAMKKRGRPAGSKKELVSLRLDKSVLEAFRASGKGWQSRINQVLSDFVKQV"
     gene            complement(11279..11569)
                     /locus_tag="PWY43_000947"
     CDS             complement(11279..11569)
                     /locus_tag="PWY43_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL2135840.1"
                     /translation="MKIYTWDETKRKINLAKHGLDFADADEVLSSPYRFDVEVIRNNE
                     FRIQSFAYSFGRLLVLSLAYTEREDYIRVISFRRASKAEREAYYEYLQQYFE"
     gene            complement(11707..12246)
                     /locus_tag="PWY43_000948"
     CDS             complement(11707..12246)
                     /locus_tag="PWY43_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDL2135841.1"
                     /translation="MLDLTKIKLVITDVDGVLTDGGMYYTDQGEVMKRFHVHDGLGVK
                     MLQSCGIKVAVLSGGDTALLRKRLEVLKIDLALLGKMEKRSACFELMQQAGVTPEQTA
                     YIGDDTLDLPAFEVCGIAIATRNALDYIKEKADWVLQKAGGEGAFREVSDQILEAQGF
                     GEIFKTADGFFTVAGKMAQ"
     gene            12317..13000
                     /locus_tag="PWY43_000949"
     CDS             12317..13000
                     /locus_tag="PWY43_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL2135842.1"
                     /translation="MSIAENLSQIHQQIQQISQQHQRDDVRLLAVSKTKPVEAIQEAI
                     EGGQRAFGENYVQEAIEKIECFANRSDLEWHFIGPLQSNKTKLVAAHFDWIQTVDRLK
                     IAERLSEQRPIGKAPLNVLIQINISDEASKSGIEPDEMLSLAQAISQLPNLKLRGLMA
                     IPKPESEPEQQKIAFCKMQQLFHRLQAEFEGIDTLSMGMSDDMQAAIECGSTMVRIGT
                     AIFGARDYR"
     gene            13191..15038
                     /gene="typA"
                     /locus_tag="PWY43_000950"
     CDS             13191..15038
                     /gene="typA"
                     /locus_tag="PWY43_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL2135843.1"
                     /translation="MQNVDINKLRNIAIIAHVDHGKTTLVDKLLQQSGTFGDTRGDVD
                     ERVMDSNDLEKERGITILAKNTAINWNGYHINIVDTPGHADFGGEVERVLSMVDSVLL
                     VVDAFDGPMPQTRFVTQKAFAHGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLGATD
                     EQLDFPIIYASALNGVAGLEHEELAEDMTPLYEAIVKHVQPPQVELEAPFQMQISQLD
                     YNNYVGVIGIGRIKRGSVKPNQAVTVVGSDGKTRQGRIGQVLGHLGLQRFEATEAFAG
                     DIVAITGLGELNISDTICDINAVEALPALSVDEPTVTMFFCVNTSPFCGQEGKFVTSR
                     QILERLNKELVHNVALRVEETPNPDEFRVSGRGELHLSVLIENMRREGYELAVSRPKV
                     IYREEDGKKQEPFEQVTIDIEEQHQGSVMEALGIRKGEVRDMIPDGKGRTRLEYVIPS
                     RGLIGFRNEFMTMTSGTGLLYSTFSHYDDVKPGEIGQRKNGVLISNATGKALAYALFG
                     LQERGKLMIDHGVDVYEGQIIGIHSRSNDLTVNCLQGKKLTNMRASGKDDAIVLTTPV
                     RFTLEQALEFIDDDELVEVTPQSVRIRKRLLTENDRKRASRTTTSTSTH"
     gene            complement(15087..15524)
                     /gene="rimI"
                     /locus_tag="PWY43_000951"
     CDS             complement(15087..15524)
                     /gene="rimI"
                     /locus_tag="PWY43_000951"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2135844.1"
                     /translation="MIEQIQAEDFEQLFEIEQQAHLVPWSKGTLLNNQGEKYLNLKVV
                     EQNKIVAFAISHIVLDEATLFNIAVAPDFQGKGIGKRLLSELILQLQQRKLTTLWLEV
                     RESNHSARKLYDSLGFNEVTTRKNYYPTPNGSKENAVVMALYL"
     gene            complement(15517..15939)
                     /locus_tag="PWY43_000952"
     CDS             complement(15517..15939)
                     /locus_tag="PWY43_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDL2135845.1"
                     /translation="MNRRDLLLQEMGISQWVLTNPQVLKGDAQIRLNNAVKLVVVCEE
                     DHQHSRLFADILRALKLKNSEYQWLTGEQSQRLVFEHSPIFWLIQAEEQAVKMAKSFT
                     NQTAWKNESWQDLAKSTTKRQLWQQIDTFSAQFGEQRD"
     gene            16068..17057
                     /gene="rsmC"
                     /locus_tag="PWY43_000953"
     CDS             16068..17057
                     /gene="rsmC"
                     /locus_tag="PWY43_000953"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDL2135846.1"
                     /translation="MLSLESEVLERHLALFENKSVLLFGDVRDDFARQLQSRAEKVAV
                     FSSYFDYARHHSEVEFGLECGLSAELAVFYWTKNKQECQFQLLQWLSQCQAGQEMLII
                     GENRAGVRSVEKLLEPFGSIAKIDSARRCGLYHFELQTVPTFDCKKFWKSYRLQDLDV
                     FALPAVFSSAELDNGTKLLLSTFDKADRLKGKVLDLGCGAGVIGATLKQQFPKIKLTM
                     SDIHAMALESGRRTLAENQLEGEVVASDVFSHINERFDLIVSNPPFHDGVDTAYRAVE
                     ELIFQAKNHLTKGGELRIVANAFLPYPDLLDKAFGKHDVLAKSTKFKVYSART"
     misc_feature    17187..17309
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            17331..17804
                     /locus_tag="PWY43_000954"
     CDS             17331..17804
                     /locus_tag="PWY43_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL2135847.1"
                     /translation="MKNPIEIYQSQDGKTQVSVQFDKETVWLSLQQMADIFGRDKSVI
                     SRHLRNIYQEGELEREATVAKNATVQIEGAREVVRQVEFYNLDAIISVGYRVNSKAGT
                     QFRIWATARLKDYLVKGYALNERRLQERGIEFEQVIGLLSQYTGCLSLVGGRKFA"
     gene            17871..18311
                     /locus_tag="PWY43_000955"
     CDS             17871..18311
                     /locus_tag="PWY43_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2135848.1"
                     /translation="MSINISPLASKNLTEWKLLWQQYLDFYQVNLADDIIQSTWQKVS
                     ESENVKGFGAFVDNRMVGFVHIVIHPNTWNLTECCYLEDLFVLAEYRNQGIGKELIEY
                     IYRYAQEKGYNRVYWVTDKNNHQAQQLYNKLANDSGIIQYRKNF"
     gene            18341..19408
                     /gene="hisC"
                     /locus_tag="PWY43_000956"
     CDS             18341..19408
                     /gene="hisC"
                     /locus_tag="PWY43_000956"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL2135849.1"
                     /translation="MTISQLSRKNIQALTPYQSARRLGGSGDVWLNANEYALSPNFDL
                     TDRTFNRYPEPQPQAVIEGYARYAGVAPENVLVSRGGDESIELIIRAFCEANDSILYC
                     PPTYGMYAVSADTCGIATKTVPLTADFQLDLAQIKANLEGVKVVFVCSPNNPTGNLIK
                     RSDLFELLQITAGKAIVVVDEAYIEFCPEASLVSELANFPHLAIIRTLSKAFALAGLR
                     CGFTLANAELIGVLQKVIAPYPLPVPVSDIAAQALSPQGIEQMKTRVAEVITLRAELQ
                     KNLENLPLVEQVFDSEANYLLFKCQDGQKVFKALWEQGIILRDQHKALGLQNCIRITV
                     GAREENLRVVEAITALSQFQR"
     gene            19587..20675
                     /gene="hisB"
                     /locus_tag="PWY43_000957"
     CDS             19587..20675
                     /gene="hisB"
                     /locus_tag="PWY43_000957"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDL2135850.1"
                     /translation="MQPTLFIDRDGTLIDEPKTDFQIDSLEKLKFEKNVIPALLKLKG
                     KYRFVMVTNQDGLGTSSFPQADFDKPHNAMMALFNSQGIEFDDVLICPHKPEDGCDCR
                     KPHTKLLQKYIDRQLFDPANSFVIGDRATDVQLAENLGIRALQYHPEKLNWDLIAEKL
                     LGEAVTNIGERPKRFAEVIRKTKETDIKVQVWLDETGNNEISTGVGFFDHMLDQIATH
                     GGFRMNITTKGDLWIDEHHTVEDTALALGTALKQAIGDKRGIARFGFVLPMDESRAEC
                     AMDLSGRPFIKFKADFKRDKVGDFSTELTEHFFQSIAFTMLATLHIKAKGDNDHHKIE
                     SLFKVFGRTLRQCIRIEGNELPSSKGVL"
     gene            20770..>21225
                     /locus_tag="PWY43_000958"
     CDS             20770..>21225
                     /locus_tag="PWY43_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135851.1"
                     /translation="MIREVIDFIKNNKWYVLLVVLLLGLYFYQFHGGLSSDSEKWDHF
                     GSYIGGIFSAVTLLSVLHGMQLERKRFKEQKEEFENDKRVLEKRRRKEDFERTFFMML
                     EQHNLKISSIEGNIDNVYDMLITAYRFENARIYCEVGRLNKEYANINSYF"
BASE COUNT         6411 a         4141 c         4721 g         5952 t
ORIGIN      
        1 tgattaagct agccatatca tttcatttgc aaaaatcggt gaaaaaagac cgcttgtagg
       61 caagcccatt gacaagcggt cagattttag ggatttttta caaaactgtt tatttgcgaa
      121 tgagtttctt ctcgacagtt tcttgataga acgctacaat aaccgtctat ttgtatatct
      181 tttttatatc cctaaaccga ggtttaagaa tgtcattttc aattgaaaca ttagaaggct
      241 taaaacgccg tgttactatt aacgtaccag ctgataaaat cgaagcagca tacaaagaac
      301 aattaaaagg ctatgcgaaa aatgctcgtg tagatggctt ccgtaaaggt aaagtgccac
      361 actcaattat cgaacaacgt tttggtattg cagcccgtca agatgtgtta tctgatgaaa
      421 tgcaacgtgc attctttgaa gccgtcattg ctgaaaaaat caacttggca ggtcgcccta
      481 ctttcacgcc aaacaactac caaccaggtc aagacttcag ctttactgcg gaatttgaag
      541 tattcccaga agtggaatta aaaggtttag agaatatcga agtagaaaaa ccggttgttg
      601 aaatcgcaga cgctgattta gacaaaatgg tagatgtatt acgcaaacaa caagcgactt
      661 ggtcagaaag ccaagaggct gcaaaagccg aatctcgtgt aactattgat ttcgttggtt
      721 cagtagatgg tgaagaattc gaaggcggta aagcatcaga cttcgtgtta tttatggggc
      781 aaggtcgtat gattccgggg tttgaagacg gaatcgtagg tcgtaaagcg ggtgagcaat
      841 tcgacattga tgtgactttc ccagaagaat accacgctga aaacttaaaa ggcaaagcag
      901 cgaaatttgc aattaccttg aaaaaagtag aagagatggt attaccggaa ttaaccgaag
      961 agtttgtgaa aaaattcggt aatgcaaaat cagtggaaga attacgcgca gaaattaaga
     1021 aaaatatgca acgtgaactt aaaaatgcag tcacttcacg tgtgaaaact caagtaatca
     1081 acggcttaat tgctcaaaat gatgttgaag tgccggctgc tgcggttgaa gaagaaatca
     1141 acgtattacg ccaacaagcg gtgcaacgtt ttggtggtaa gcctgaaatg gcagctcaat
     1201 taccggctga attatttgaa gcagaagcta aacgccgtgt tcaagtgggc ttattattat
     1261 cttcaatcat cggttctaac gaattaaaag tagatgaaaa acgtgttgaa gagatgatcg
     1321 cagatatcgc ttcagcttac gaacaaccgg ctgaagtggt tgctcactat gcgaaaaacc
     1381 gtcaattaac tgaaaatatc cgcaatgtgg tattagaaga gcaagcggtt gatgcagtgc
     1441 tagcgaaagc gaaagtgact gaaaaaacaa tgtcatttga cgaagtaatg tcacaacaag
     1501 ctcaataatt tccttataag gtgggctttt gctcaccttt tattactttt acaagcggtt
     1561 aaatttagtg ataaatttgc atcaaaacga ccgcttattt tttgatgtta aggaaattga
     1621 attatggaac aactaacttt tgctcaacaa ttagagcaat tttttgccaa tcataccttg
     1681 atggtgtttg cttgggttgc attattgatt gcggtaattt ttagctttta taaaagtgca
     1741 acgcaaaaat ataaaattgt cgataacgca caggctacac agctgattaa taaagaagag
     1801 gcagtagtgg ttgatttacg ttctgatgaa gagttccgtg ccggtcatat tattgatagc
     1861 attcacgtgt taccgtcaga cattaaagca gctaaaatca atcagattga taaatacaaa
     1921 gctcgcccgg taattgtagt agatactaat ggcttcactt caggtggtgt tgcggcagat
     1981 cttgctaaac aaggctttag cagagtttat gtattaaaag aagggattgg cggctggcgt
     2041 gcagcaagcc taccaactat taaaaaacat aaataatcta ttattaagga aattttatgt
     2101 cagaagaaaa tcaagttgca tcaacagaag aagctacaca aacccctttt gagcttcaaa
     2161 ttcaacgcat ttatgtaaaa gatgtttcat ttgaagcacc aaatttaccg actattttcc
     2221 aacaagagtg gaaaccacaa ttaggctttg agttagatac tgaaaccaaa gaaattggtg
     2281 aagatttata cgaagttacc ttacacatta cggtgcaaac tacgttagaa gacagcaatg
     2341 atgtcgcttt catttgtgaa gtaaaacaag caggtgtgtt tactattaaa ggtttagaag
     2401 gcattcaact tgctcactgc ttagcttcac aatgtccgaa tgtgctttat ccttatgctc
     2461 gtgagctaat ctcaagcctt gtgaatcgtg gtacattccc ggcattaaat ctctcaccgg
     2521 taaactttga tgcgttattt atggattatc tccaacgcca agaagaaagt gcagaagagc
     2581 cggctttagc gaactaagca ttttagatag aaaataggta tgcaaggcat acctattttt
     2641 ttacaagcgg ttattttttc tactattttt tgcaattttc taaataagcc gaacagagga
     2701 aatatgagtg aaatctattc tgccccaatc acggtattag gtgcaggttc ttatggaaca
     2761 gctttagcca ttgccctttc tcgaaatggt cacaaaacct atttatgggg gcataatccc
     2821 gacaaaatgg ccgttttggc atcagagcga atgaaccaag attttctgcc tgatattgta
     2881 ttcccggaag ccttagaaat tgtcgcagat cttgctacag cggtacaaaa atcgaaagat
     2941 ctgctgattg tggtgccaag tcatgtattc tctgaagtat tggctcaaat caagcctttt
     3001 ttgtcccaag atcaccgtat aatgtgggca accaaagggc tggagcgaga taccgggcgt
     3061 ttgttgcaaa cggtagctaa ggaaattttg ggggaaattt acccgcttgc ggtgctttcc
     3121 ggtccgactt ttgccaaaga actggcggca ggcttgccaa cagcgatttc gcttgcctca
     3181 actgatcctg tttttgccga tcaaatgcaa aaacggattc actgttccaa agcgttccga
     3241 gtttacctca acaatgatat tatcggtgtg caactcggtg gggctattaa aaatgtgatt
     3301 gcaattggtg ccggtatttc agacggaatg ggattcgggg cgaatgctcg taccgccttg
     3361 attactcgag ggctggcgga gattagccgt ttgggtgttt cgctcggggc tgatccgaaa
     3421 acctttaccg gaatggcggg gcttggagat ttagtgctga cgtgtaccga taaccaatcc
     3481 cgaaaccgcc gatttggttt ggcgttaggg caaggaaaat cggcagaagt cgcgatagcg
     3541 gaaatcggtc aagtggtaga aggattttat aataccaaag aagcctattt gctcgctcaa
     3601 acacagggcg ttgaaatgcc gattgtagaa caaatttacc aaatgttatt ttgtggcaaa
     3661 caggcttcgg atgtggtgaa aaccttgttg gggcgtgaac gaaaaggcga ataaagaaag
     3721 gagaataaaa tgcagcagag tgaaattaat cagatttggc aagcaattcg agatgaagcc
     3781 aaagagctgg cgaattgtga acctatgtta gcaagttttt tccacgctac cattttaaaa
     3841 caccataatc taggcgatgc gttaagttat attttggcga ataagttaga aaacccgatt
     3901 atgccggcaa tcgccttaaa agaaattatt gaggaagctt atcgagcaga gccacaaatt
     3961 attgctagtg cagcttgtga tattaatgcg gtcagaacta gagatcctgc ggtggacaaa
     4021 tggagtacac ctctgctgta cttaaaaggc ttccacgctt tacaaagcta tcgggtgacg
     4081 cattatcttt ggaatcaagg caggaaagca ttagcggttt atttacaaaa tgaaatttct
     4141 gttgcctttg atgtggatat tcatcctgcg gcaaaggtcg gttgcggaat tatgtttgac
     4201 cacgccaccg gtattgtggt gggtgaaacc tctgtgatcg aaaatgatgt ctcgatttta
     4261 caaggtgtaa cactaggcgg tacagggaaa gaaaatggcg accgccaccc gaaaatccgt
     4321 gaaggcgtga tgattggagc cggtgcaaaa attctcggta atattgaaat cggacgctat
     4381 tctaaaatcg gggcaaattc agtggtgcta caacctgtgc ctgatcacgc cacagcagca
     4441 ggtgtaccgg ctcgcattat cggcaaatcc tctgaacaaa aaccggcttt tgatatgaat
     4501 caatactttg aagatgagca aggtttgttt ggtgatggga tttaggtaag agttattcct
     4561 ttaatttatg atttagacat tgctaagtac aagttgcaat taacctttat tcaaatgttt
     4621 tgcccgctct taagcgggct tactattttc actatttgct atgtatttcc tatcgttgta
     4681 attgaatgct tattttgtca tcaattaatg ctagaattcg ccaaattcta ttttttggat
     4741 gataaatata tgaaaccagc ctcttttaaa ctctctttaa tttctgcatt cgtagggctg
     4801 tcattggcac agcttagtta tagttctacg gttcgtggtg atatagatta ccaatatttc
     4861 cgagatcttg cggaaaataa aggaaaattt tatgtcggag cgaaaaatgt tgaaatattt
     4921 aataagcaag gtgacaagat aggtattgct ttagaaaata ttccaatgat tgacttctct
     4981 gtgattacac gaggaggagg ttatgcaacc ctgactaaac cacaatatat tgtgagtgta
     5041 gagcacaaca gaggctatcg aacagtgcag tttggtgatt ctggtagtga aaacccagat
     5101 aatcaccatt ttaattatca attggtttca cgcaatgatt atgatccaaa agggagtaaa
     5161 gccattcagg gatttacgca taatgactat caaacgcctc gtttgcataa actagtaaca
     5221 gaaacagcac cagcagagca atcccaagag aaaaattatg atctgtatcg tgatgcaagt
     5281 atttttccaa tgtttattcg tgcaggttca ggtacacaat acattttagc tgaaaagaat
     5341 gctgtaaagg aaaaacttgc ggatccgtat gcttacctaa ttggtggaac cacattaaag
     5401 ttaacgagca ataatgctaa tacctttgtt tttaaaggca atgtatttga tgatagttac
     5461 agccctctta gctctcatcc acagcaaggt gatagtggaa agactttcac ttggactgca
     5521 cttggaaata gtagcaggat tacgggggcc acggctgaat tagtagttga tttagctgat
     5581 acctcattgc ctgacccaac aacaggtaag ccacataagc catctttaca ccatgggaaa
     5641 acgcttaaaa tttccggaga tggcaatacc cttaatttag ctaataatat taatcaggga
     5701 gccggagcgc tatatttttc aggtaatgcg gttgtaaccg gtgctaatga aatgacaacg
     5761 tggcttgggg ctgggatatc tgtagataaa aataagaatg tagaatggca agttcataat
     5821 ccggtgggag atagattatc taaaattgga gaaggaacgc taactgtttc tggtaaaggc
     5881 aagaatttag gatcaattag tgttggagat ggcacagtga ttcttaacca acaagcgggc
     5941 gaaaatggtg aaaaatcggc atttagtgaa gttgggatcg ttagtggacg accaacagta
     6001 atcttaaata gcgctgatca agtagatcct aacagtattt attttggtta tcgtggcggt
     6061 cgtttagatt taaacggtaa cagcttaaca tttaatcgga tccagaatgt cgatgatgga
     6121 gcgagaattg ttaataataa tgcaggtact gctgcgaata tttccttagt aggccaagta
     6181 tttacagcta atgatgttcg tacaattaat tttggagaag gctataatgc tgatctgttt
     6241 cgtagccaag gcgcctattt tgtgcttgag aacaatgcgt ggaagtttat aagttggaat
     6301 catgatgatg ctaaaaagta tgtcgttgaa aagaaaaata aagcactaga aaatcaactt
     6361 tatgcttaca atggctattt tggcgaatct gactcaagca gagagaacgg aaagttaaac
     6421 attcacttca agcctattaa tgcagggggg aagctccttt taactggagg taccacacta
     6481 gatgggattt tgtcagcaga gaatgatgct gaaattattt tatccggtcg cccgattcca
     6541 tatgcacgcc aagttcttga tgaaaataaa aaaacaatta ataaggaaat tataaaagaa
     6601 ggtgaatgga ttaatcgaac ctttagcgca acaacctttc aagctaagga taatgggaaa
     6661 attgaagtta gccgtaatgt agttgaagta aacggtaatt ttaatttgag tgacaatgca
     6721 acagctcaag ttggtttcac acaggggaaa tctcaatcat gtgttcgtta ggatcgtacc
     6781 ggcatagcaa gttgtaattt atctgtgtta tctgataagg atttacatag ttggcaacgc
     6841 actacagtca atagtgatat taaattaact cagaatgcac gttttgaatt aggtagtaag
     6901 gctgatctta ttgggacaat tgaaagcaat ggcgatagcc aaataaactt gcgaaatggt
     6961 tctagctggg taatgacagg aaacagcaat gtcaataaat tgaatgtaga taatgcgaca
     7021 atcaccttag acaataatgt gggtgagcca aatactttaa atattaatag ccttagcgga
     7081 agtggggtta ttaactttat cacttacttt gctcaaacaa taagtgattt gataaatgta
     7141 gagcatgcaa gcggtgcttt taaggcaaaa atttcccaaa ttggcacacc cactacgcaa
     7201 aggggcgttt tatgatcttc tataaataca ttaatttgaa gaaggctagc cacctcaatt
     7261 ttaaggtggc taaatatatt gatgaacaag ggaaatcaag aaagatgtat ttaatgaccc
     7321 aagacggctt tacgctttta gtgatgggct ttacccacaa atgaaccgca cttttgccct
     7381 aaataaaatc gccgttgagc ggtgtaagca ggatttcccc gactgctacc actttaaaaa
     7441 gctgatggcg gaggaatgca ccgaactcgc cgaccgtttg aaaatggggc ttgtactgat
     7501 ggcggaatta aaaacaacaa gcaccctaac aagcgaccaa aaccgccatt ttaaagcgtt
     7561 cagaaatggg gctaagtatt taatcaaaca gcttgaataa acggctatac acgttgccaa
     7621 cgtggacaag ggcgaatttg agccgacacc atataacccg attaaacaag cggagggaaa
     7681 tccgcccaca cttggagcag tttaaaacgt tgctctaggc gttcgcctct atcataggcg
     7741 gctttgattc ggtcgctgtt taaatgcgat aaggcaacct cgattaaatc gctattgtag
     7801 cctttctcat taagataagt ggaggcgatg gcccgcaagc cgtgagccac taatttgccc
     7861 ttgtagccca ttcgtttgat cgcaccgttg gcggtttgag tattgacgtg cgtttgtggg
     7921 ttcttcacgc tagggaataa atacgtttta ccgccactta gctttttaat ctctcgcaaa
     7981 agtgcgattg cttggcgtga gagtgtgatt ttatgctcaa tgcctcgctt catattttcc
     8041 gccggtatcg tccacagttt ggctttttcg tctatatccg cccattttgc cttagccgct
     8101 tcattcgggc gggtcattgt gagtaactgc cacagaatta aatagcgtgt ctgtaaatga
     8161 atttgggcgt tttgtaaggt gtataaaaac tcgcctaaat cttccggctt aatggttttg
     8221 aaatgttcaa ccgttgggct atcaaactct ttcgcaatat tggcggtagg gttaaacggt
     8281 atcatttcac ggtgtacggc aaacatcata atttcattat gtttttgaat tgcccgtttt
     8341 aacttctcta gtttgccctc gtcttggtat tgcttgaatg ccttgattgc cattggtgcg
     8401 gttatttgtg tgataggcat tcttccaaag tgcggcaaga ggttattttt aattaaggcg
     8461 attacatctt tttggtagtt gtctgaaaaa ttcttcttgg tcttgcggtg ttccagccac
     8521 gcccacgcta cattttcata agtgttgctg atttcgttgg cctttgcctg ttcctgttgt
     8581 tggcggtggg tttgagggtc gatattttga gccaataatg ccctatattc ttctctaatt
     8641 tctctcgctt gtgctaaaga aatatcaggg taagccccaa tgcttaaatc attccgcttt
     8701 tttgtgaacg gtctttcata cctaaaccgc cacaatttag aatttgaggt aataacaagt
     8761 agtaagccgt taccgtctct taatgggctg tcttccggtt tcgcttttct tatttgggta
     8821 tcgtttaacg gtgtgatttt tctagccata gtaaaccttt aggagtataa agcctaaatc
     8881 ttaggagtac gaatttttgg gggtatttcg gcgtactcct aaatatactc ctaaaatctt
     8941 cggatttgat tgtatcgtat aggtacgcaa cggacaataa aaaagccttg aaacattgat
     9001 tttcaaggct ttttaggtat cggttgaacc gtataggatt ataatttggt ggagctgggg
     9061 ggatttgaac ccccgtccga aatttctcta ccttcagtac tacatgttta gtctagtctt
     9121 taatttcgct tattcacagc ggacagacac gcaatgaata aactagcttt atttaattta
     9181 gtgtttcgat cctaaagcgg cggcgtccac acgatctcgt tttgggtgac ttatctttgc
     9241 cctcgtctta cgagcggaag ctgaggagat aaggctttaa agcaggttat taagctgcta
     9301 aagcgtattg ttcgtcgttt gcgactatta ttttgcggtt tgtttacgag gcctaccgca
     9361 cctcgacatg caccttgggc ttcgccaatc ccgtcgaatc caaaatcagc cccaaaagta
     9421 gcggtattct aaaggaaagc ggtcgaattt gcaaaaagtt ttgcaagtgg cggaacttag
     9481 cggtattgaa tagctcgaaa taaaatcata caaatcccac accagataga tttaacgatg
     9541 ttataatgta aaccttgctg agagtgaaga gaaatagaat gaaaaaattt aacgagaacg
     9601 agattgaaga tgctgaaatt atagatgccg agccgattga tgttgatgag atagacgaag
     9661 aagaaacatc ggtagaagca atgcatccgg ctttggtttc cggctcaatg cctgtgccgc
     9721 agggcaattt ggatagctac atccgaatgg caaatcagta tccgattctc accgccgagc
     9781 aagaaaaaga actggcggag cgttattatt atgatgaaga cgttgaggcg gcaaaacagc
     9841 tgatcctttc ccatatgcgg tttgtgatcc atattgctcg tggctatttg ggctatggtt
     9901 taccgctggc agatctgatc caagagggta atatcggctt gatgaaagcg gtaaaacggt
     9961 ttgacccgaa tgtcggcgtg cggttggttt cttttgcggt tcattgggtg aaagcggaaa
    10021 ttcacgaata tgtgctgaaa aactggcgaa ttgtgaaagt ggcaaccacc aaagctcaac
    10081 gtaaattatt ctttaattta cgcaaaaata aaaaccgctt ggcgtggttt aatgaagaag
    10141 agatcaaaaa agttgccgat gatttagggg tttcagtgga agaagtacga gaaatggaat
    10201 ctcgaatgac cggacaagat ttaggttttg atctgcctgc cggtgatgac gatgaagaca
    10261 gctacgctcc ttctatgtat attgaagata gcagctccaa tttcgccgat gacttggaag
    10321 acgaacaaca taatgggcag gcaaccgctc aacttgccta tgcgttagcc acccttgatg
    10381 aacgtagcca agacatcatc aaaacccgct ggttagatga caataaagcc acactgcaag
    10441 atttagccga taaatataac atctcggcag agcgggtaag acagcttgaa aatgccgcat
    10501 tgaagaaaat caaggaagcg atttcattag acggctagaa atgcctacaa gcggtgattt
    10561 ttttactcgc tacgctcgca cttcgtgccg tcggcagagc caacgttcaa acgcaagcgt
    10621 ttgtgcaaaa ttttacgcaa aaagcaccgc ttgtattttg actaaaaatg aggatactat
    10681 gattaacatt accgataaac aaattgaccc aactttctac cagcgagcag acggctttat
    10741 taacgttgcc aacgctcatt taaaaaacat tgccccaaac caagtcagca atgcgatgtt
    10801 gtttggttgt gccagattta acgcctatgt tgcggcaagc aaggcggaat ataaacagca
    10861 gttggccgat tccagagaag aagttattca atattttgtg gagcaataca aagaaatgct
    10921 cactgcaaat ttagatgaat atatccaaaa cttcgagcga tatattgaag ggaaaaaggc
    10981 ggattaaccg cctttgtttt gtggtcaaac ttgttttaca aaatcactta aaacttgatt
    11041 aattctactt tgccaacctt tacctgaagc tctaaaggct tcaagaacgc ttttatcaag
    11101 acgtaacgaa accaattctt tctttgaacc agctggtctg ccacgttttt tcattgcaat
    11161 tgaaaactct gatggcgtaa ccgggcgttc atcgtcatct tttccgtccc aaataaaatc
    11221 ttggcttgcc ggaatagctt gaatagccat aagtagctgt tttttctcta aatttttatt
    11281 attcaaagta ttgttgtaag tattcataat atgcctctct ttctgcttta gatgctcgcc
    11341 gaaagctaat tactctgatg taatcttccc tttctgtata tgcaagtgat aatacgagta
    11401 aacgtccaaa tgaatatgca aaagattgta ttctaaattc attgttacga ataacttcaa
    11461 catcaaatcg atagggcgat gagagaactt catctgcatc ggcaaaatct aatccgtgct
    11521 tggctaaatt gattttgcgt ttagtttcgt cccaagtata aattttcata attattgtat
    11581 atacataaaa taaatctaac aagaactaaa tttaatttag caaacagtaa tttgaaaatc
    11641 agattagaaa tacatttttt cgatgcagat cgcaaaaata aaatgaaatt taactgcttg
    11701 aagagtctac tgagccattt tccccgctac cgtaaaaaaa ccatctgcgg ttttaaaaat
    11761 ttcaccgaat ccttgagcct ccaaaatttg gtcggagact tcacgaaacg ccccttcacc
    11821 accggctttt tgcaataccc aatctgcttt ttctttgatg taatcgaggg cgttacgggt
    11881 tgcaatcgca atgccacaca cttcaaaagc cggtaaatct aaggtgtcat caccaatgta
    11941 agccgtttgc tcaggggtaa caccggcttg ctgcattagc tcaaagcagg cggagcgttt
    12001 ttccattttg cctaacaaag cgagatcaat tttcagcacc tctaaccgtt tgcggagcaa
    12061 agccgtatcg ccgcccgaga gtactgccac tttaatgcca caactttgta acatcttaac
    12121 gcctaaacca tcgtgaacgt ggaaacgttt cattacttcg ccttggtcgg tgtagtacat
    12181 tccgccatcg gttagcacgc cgtccacatc ggtaatcact aatttaatct tggtgagatc
    12241 taacattttt ttgcctcaaa tatggtatga ttttgccatt atagcttttt tacctccgtt
    12301 taccaaagga accgaaatgt cgattgccga gaatttatcg caaatccatc aacaaattca
    12361 gcaaatttcc caacaacacc aacgtgatga cgtacggcta cttgccgtct cgaaaaccaa
    12421 gccggtagaa gccattcaag aggcgataga gggcggtcag cgtgcatttg gtgagaatta
    12481 tgtgcaggaa gcgatcgaaa aaatcgaatg ttttgcaaat cgttcagact tggaatggca
    12541 ctttattggt ccgttacaat cgaataaaac caagttagtt gcagctcatt ttgattggat
    12601 tcaaacggtg gataggctca aaattgccga gcgtttaagc gaacaacgcc cgataggaaa
    12661 agcaccgctt aatgtattga ttcaaattaa tatcagcgat gaagcctcaa aatcaggcat
    12721 tgagccggac gaaatgttat ctttagccca agcgatttct caattaccga atttgaaatt
    12781 acgtggttta atggcaatcc caaaaccgga aagtgagccg gagcaacaaa aaatcgcttt
    12841 ttgcaaaatg cagcaacttt ttcaccgctt gcaagcagaa tttgagggca ttgacaccct
    12901 ttcaatggga atgtctgacg atatgcaagc cgccattgaa tgtggttcta caatggtgag
    12961 aataggcacg gcgatttttg gggcgaggga ttatcgctaa aatttatcgg cataacaaag
    13021 gaatgaatct ggcaagtagt cttaaattgt aaaatttttg caaatttaga ccgcttgttt
    13081 tgtagccaaa ttctgaaaaa ctctgtataa ttcgacctcc aatttctaga ctctcgccgg
    13141 ttttgatggg cgagagtttt tattttcact caataaaaaa agatattcca atgcaaaatg
    13201 tagatattaa taaattgcgt aacattgcaa ttattgctca cgttgaccac ggtaaaacaa
    13261 cgctggttga taaactttta caacaatccg gcacctttgg tgacacccga ggcgacgtgg
    13321 acgagcgtgt gatggactcc aacgatttgg aaaaagagcg tggtattacc attcttgcaa
    13381 aaaatacggc aattaactgg aatggttatc acatcaatat cgtagatacc ccagggcacg
    13441 ctgacttcgg tggtgaggtt gagcgtgtac tttcaatggt agattcggtg cttttagtgg
    13501 tagatgcctt tgacggcccg atgccacaaa cccgtttcgt tacccaaaaa gcctttgctc
    13561 acggcttaaa gccaatcgta gttattaaca aagtggaccg ccctggcgct cgtcctgact
    13621 gggtggttga tcaagtgttc gatctcttcg taaatttagg tgcaacagac gagcaattag
    13681 atttccctat catttacgct tctgcgttaa acggcgtggc tggtttagaa cacgaagagt
    13741 tggcagaaga tatgaccccg ctttatgaag cgattgtgaa gcacgttcag ccgccacaag
    13801 tggaattaga ggctccgttc caaatgcaaa tctcgcaatt agactataac aactatgtag
    13861 gcgttatcgg tatcggtcgt atcaaacgtg gttcggtaaa acctaaccaa gcggtaacgg
    13921 tagtaggcag cgatggcaaa acccgccaag gtcgtatcgg tcaagtatta gggcatttag
    13981 gtttacaacg ctttgaagca actgaagcct tcgcaggcga tatcgtggca attacaggtt
    14041 taggtgaatt aaatatttca gatacgattt gtgatattaa tgcggtggaa gcgttacctg
    14101 cattaagcgt agatgagcca acggtaacca tgttcttctg tgtaaacacc tcgccattct
    14161 gtggtcaaga aggtaaattt gtaacttctc gccaaattct tgagcgttta aacaaagaat
    14221 tagtccacaa cgtagcactt cgtgtggaag aaacacccaa cccagacgaa ttccgtgtgt
    14281 caggtcgtgg cgaattacac ttatcggtat taatcgaaaa tatgcgtcgt gaaggttacg
    14341 agttagcagt gtctcgcccg aaagtaatct accgtgaaga agacggcaaa aaacaagagc
    14401 cgtttgagca agtgacgatt gatatcgaag agcaacacca aggttcagtg atggaagcac
    14461 ttggtatccg taaaggtgaa gtgcgtgata tgattccgga cggcaaagga cgcactcgct
    14521 tagaatatgt gattccaagc cgtggcttaa tcggcttccg taacgaattt atgacgatga
    14581 cctcaggtac aggtttactt tactcaacat tcagtcatta tgatgacgta aaaccgggcg
    14641 aaatcggcca gcgtaaaaac ggtgtgttaa tttctaacgc aacgggtaag gcattagcat
    14701 acgcattatt tggcttacaa gagcgtggaa aattaatgat cgaccacggt gttgatgtat
    14761 atgaagggca aattatcggg attcacagcc gttcaaacga tttaaccgta aactgcttac
    14821 agggtaagaa attaaccaat atgcgtgcat caggtaaaga cgatgccatc gttttaacca
    14881 caccggtgcg tttcactcta gaacaagcgt tagagtttat tgacgatgac gaattagtgg
    14941 aagtaacacc acaatcagtg cgtattcgta aacgtttatt aactgaaaac gaccggaaac
    15001 gtgcaagccg tactacaacc agtaccagca cgcactaact gataaaaaag agcgactgag
    15061 gtcgctcttt tggttttaaa ttctccctac aaatataacg ccattaccac cgcattttct
    15121 ttgctaccgt ttggcgtggg gtaatagttt ttgcgggtgg taacttcatt aaaaccgagt
    15181 gaatcgtaga gttttctggc agaatggttg gattctcgca cttccaacca cagggtggtg
    15241 agtttccgtt gttgcagttg caaaattaat tcggaaagta accgcttgcc aatgcctttg
    15301 ccttgaaaat caggagcaac tgcaatgtta aataaggttg cttcatctag cacgatatga
    15361 ctaatggcaa aagccacaat tttgttttgc tctaccactt ttaaattcag gtatttttca
    15421 ccctgattat ttaataacgt cccctttgac cacggcacta aatgggcttg ttgctcaatt
    15481 tcaaataact gctcgaaatc ttccgcttga atttgctcaa tcacgctgtt ctccaaattg
    15541 tgccgaaaaa gtatcaattt gttgccaaag ttggcgtttg gtcgtagatt tggctaaatc
    15601 ttgccaagac tcgtttttcc aagcggtctg attagtaaaa ctttttgcca ttttgaccgc
    15661 ttgttcttcc gcttgaatca gccaaaagat cggcgaatgc tcaaacacca aacgttgtga
    15721 ttgttcgcca gtcagccatt gatattcgct gttttttaac tttaaagcac gcagaatatc
    15781 ggcaaagagc cggctgtgtt ggtgatcttc ctcacacact accaccaatt ttaccgcatt
    15841 gtttaggcgg atttgagcat cgccttttag cacttgggga ttggttagca cccattgcga
    15901 aatacccatt tcctgtaaca gtaaatcacg acgattcatt taaaacctca tcaaattttg
    15961 caaatagtat caaaactccg cttgaacaga aacttttttc aaaaaattca agatttctgt
    16021 acttaaattc ggtatattca cgctaatttt ttagataagg taagaaaatg ctctctttag
    16081 aaagcgaagt acttgaacgc catttggcgt tatttgaaaa caagtcggtg ttattatttg
    16141 gcgatgtgcg agatgatttt gcccgccagc tgcaatctcg tgcggaaaag gttgcggtat
    16201 tcagtagcta ttttgattac gcacgccacc atagtgaggt tgaatttggc ttagaatgtg
    16261 gtttgagtgc cgagttagcg gtgttttatt ggacgaagaa caagcaggaa tgccaattcc
    16321 aactgctaca atggctgtcg caatgccaag cagggcaaga aatgttgatt atcggcgaaa
    16381 accgagctgg tgttcgttcg gtagaaaaat tgctcgaacc gttcggcagt attgccaaaa
    16441 tcgactctgc acgccgttgt ggtttatacc attttgagct acagaccgtg ccgacattcg
    16501 attgcaaaaa attctggaaa tcctaccgct tgcaagactt agatgtgttc gctctaccag
    16561 cggtttttag ctcggcggag ctggataacg gcaccaaatt attgctttca accttcgaca
    16621 aagcagacag gctaaaaggc aaagtgttag atttaggctg tggggctggc gtgattgggg
    16681 caacgctcaa acaacaattt ccgaaaatca aactgacgat gagcgatatt cacgcaatgg
    16741 cgttggaatc cggcagacgt actttggcgg aaaatcagtt agagggggaa gtggtagcaa
    16801 gtgatgtgtt ctctcacatt aatgaacggt ttgatttaat tgtctccaac ccaccattcc
    16861 acgacggggt cgataccgcc taccgagcgg tggaggagtt gattttccaa gctaaaaatc
    16921 acctaaccaa aggcggtgag ctgcgaattg tcgcaaacgc attcttgcct tacccagatt
    16981 tgctcgataa agccttcggc aaacacgacg tactggcaaa atccaccaag tttaaggtgt
    17041 attccgcaag aacataatag gcagaacaaa cggttataaa aaagccgata tttgcttaaa
    17101 aaagtgcttg atatttctgc cggcttgacg taaattctgt tcatactgtt ttggagtaat
    17161 gcaatgaatt taaactacac tttacatcac caccacactt taacctgata tctttcgggc
    17221 atttggtgtg tgcggaagat aactcttccg agcaagtgta gcaaatcaat aaagatctct
    17281 cggaaggcaa ctttcgagag atttttttat agataaaatt tgggaataat atgaaaaacc
    17341 cgattgaaat ttaccaaagc caagacggca aaacccaagt ttccgtgcag tttgataagg
    17401 aaacggtgtg gctaagttta cagcaaatgg cagatatttt tggacgagac aagtctgtta
    17461 tttctcggca cttacgaaat atctaccaag aaggtgaact ggagcgtgag gcaactgttg
    17521 caaaaaatgc aacagttcaa attgaaggtg ctagagaagt cgttcgccaa gtggaatttt
    17581 ataatctaga tgcgattatt tctgttggct atcgggtgaa ttccaaagct ggcactcagt
    17641 ttcgtatctg ggcaaccgct cgcctaaaag actatctggt aaaaggctat gcccttaacg
    17701 aacgccgttt gcaggaacga ggtatcgagt ttgagcaggt gattggtttg ctcagtcaat
    17761 acactggttg ccttagcctt gttggtggca gaaagtttgc ctgaacaaaa agaactgatg
    17821 attaaactga ttgaacactt tattttatta aaagcataac aggaaaaaat atgagtatta
    17881 atatttcgcc gttagcctcc aaaaatctca ctgaatggaa actattgtgg caacaatatt
    17941 tagattttta tcaagtgaat ttagcagatg atattattca atcaacttgg cagaaagttt
    18001 cagagagtga aaatgtaaag ggatttgggg cttttgttga taacagaatg gttggttttg
    18061 ttcatattgt gattcaccca aatacttgga atttaactga atgctgttat ttggaagatt
    18121 tatttgtttt agctgaatat cgcaaccaag gcataggaaa agaattaatt gaatatattt
    18181 atcgatatgc acaggaaaaa ggatataacc gagtatattg ggtaacagat aaaaacaatc
    18241 atcaagcaca acagctttat aataaattag ctaatgatag cggtattatt caatatcgaa
    18301 aaaatttcta aactatgtat aaattataca ggaacacaat atgacaattt cacaactttc
    18361 ccgtaaaaac attcaagctc tcacccctta ccaatcggca cggcgtttgg ggggtagtgg
    18421 cgatgtgtgg ctgaatgcca atgaatatgc cctttcgcca aactttgatt taaccgaccg
    18481 cacttttaac cgctaccctg aacctcaacc gcaggcggtg attgaaggtt acgcccgata
    18541 tgcaggcgtt gcacctgaaa acgtgctggt ttcccgtggg ggcgatgaga gcattgagct
    18601 gattatccga gccttttgcg aggcaaacga cagcattctc tactgcccac caacctacgg
    18661 aatgtacgcc gtgagtgccg acacctgtgg cattgcgacc aaaactgtgc ctttaaccgc
    18721 cgatttccag ttggatttag cccaaatcaa agctaacctc gagggcgtga aagtggtgtt
    18781 tgtgtgcagc ccgaacaacc cgacaggcaa cttaatcaag cggtcggatt tgttcgaact
    18841 tttgcaaatc accgcaggca aagcgattgt ggtggtggac gaagcctaca tcgaattttg
    18901 ccctgaagcg agcttggtaa gcgagctggc gaatttccca catttggcga ttatccgcac
    18961 cctatccaaa gcctttgcct tggcgggttt gcgttgtggc tttaccttgg caaacgctga
    19021 gctgatcggc gtgttacaaa aagtgattgc cccgtatccg ctacctgtgc ctgtttccga
    19081 catcgccgcc caagccctct ccccacaagg cattgagcag atgaaaaccc gtgtagcgga
    19141 agtcatcacc ctgcgtgccg aattgcaaaa aaatctggaa aacttaccgc ttgtagaaca
    19201 agtttttgac agcgaagcca actacctcct attcaaatgc caagacggac aaaaagtctt
    19261 caaagcactt tgggagcaag gcattatttt gcgtgaccaa cacaaagcgt taggattgca
    19321 aaattgcatt cggattacgg tgggggcgag agaggagaat ttgagggtgg ttgaggcgat
    19381 tacagcactg agccaatttc agagatagaa tttaacgagc agtattttaa gagacattta
    19441 tagcctagca cggcatagcc gtactaggtt acgcataagt cagtcccttt gggactgaat
    19501 acagagaggc aaagtctctc actaatgctt aaccccatac gccttggcgt gtggggagag
    19561 taatgaataa gatacaggag aaaccaatgc aacccaccct ttttatcgac cgtgacggca
    19621 cgttaattga tgagccaaaa accgattttc aaatcgacag cttggaaaag ctgaaattcg
    19681 agaaaaacgt gattcctgcc ctgctcaaac tcaagggcaa ataccgcttt gtgatggtta
    19741 cgaaccaaga cggcttaggc acgagttcgt tcccacaggc agatttcgac aaaccgcaca
    19801 atgcaatgat ggcgttgttc aactcgcaag ggattgaatt tgatgatgtg ctgatttgtc
    19861 cacacaagcc cgaagacggt tgcgattgcc gcaaaccgca caccaaactg ctacaaaaat
    19921 atatcgaccg ccaactgttc gacccagcca acagctttgt gattggcgac cgtgctaccg
    19981 atgtgcaact ggcggaaaac cttggcattc gggcgttgca atatcaccct gaaaaactga
    20041 actgggattt gattgctgaa aaactactgg gcgaagccgt caccaacatt ggcgagcgac
    20101 caaagcgctt tgccgaagtt atccgtaaaa ccaaagagac cgacatcaaa gtgcaagtgt
    20161 ggctggacga aacaggcaac aacgaaatca gcaccggcgt gggctttttc gaccatatgc
    20221 ttgaccaaat tgccacccac ggcggtttta gaatgaacat caccaccaaa ggcgacttgt
    20281 ggattgacga acatcacacg gtggaagaca ccgccctcgc cctcggcacc gcattaaaac
    20341 aagcgattgg cgacaaacgc ggcatcgccc gtttcggctt cgtgttgccg atggacgaaa
    20401 gccgtgcgga atgtgcgatg gacttatcag gcagaccttt catcaagttc aaagcggatt
    20461 tcaaacgcga caaagtgggc gatttcagca ccgagctcac cgaacacttc ttccaatcca
    20521 tcgccttcac gatgctcgcc accctgcaca tcaaggcaaa aggcgacaac gaccaccaca
    20581 aaatcgaaag cctgttcaaa gtcttcggca gaacactcag acagtgcatt cgtattgaag
    20641 ggaatgaact accaagcagt aaaggtgtat tataatcacc gtaggggcgg gttttaaccc
    20701 gcccgaaagt gcggttgaaa tttgcaaagt ttttggggat tttgaccgct tgttataaag
    20761 gatatattga tgattagaga agttattgac tttataaaga ataataaatg gtatgttctg
    20821 ttagttgtat tgttattagg attatatttt tatcagtttc acgggggatt atcctcagat
    20881 agtgaaaaat gggatcattt tggtagctat attggtggta tattttctgc tgttacttta
    20941 ttgagtgttt tgcatggaat gcagttggaa cgtaaaagat ttaaggagca aaaagaagaa
    21001 tttgaaaatg acaaaagagt attagaaaag agaagaagaa aagaagattt tgagcgtaca
    21061 ttttttatga tgttagagca gcataatctt aaaatttcat caatagaagg taatattgat
    21121 aatgtttatg atatgctaat tacagcatat agatttgaaa atgctaggat ttattgtgag
    21181 gtaggaagat taaataaaga atatgcgaat ataaattcct acttt
//
LOCUS       DANKBV010000033        20568 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.033, whole genome shotgun sequence.
ACCESSION   DANKBV010000033 DANKBV010000000
VERSION     DANKBV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20568
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.033"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            189..632
                     /locus_tag="PWY43_000959"
     CDS             189..632
                     /locus_tag="PWY43_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135852.1"
                     /translation="MLIGILCIIALAVLGLLFYFHKKKRKKVNKGVNMAEYGIQTFDT
                     ANVSTFSTNERLFKYIGTKNIKYGKFSFTVTEKYEGQLVFIISMLHSVHRKQATGYKT
                     NIPNLIHLENVSINDKTISGEVVCIFPFSLPADDSDYIQIVYGAY"
     gene            633..1232
                     /locus_tag="PWY43_000960"
     CDS             633..1232
                     /locus_tag="PWY43_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135853.1"
                     /translation="MSYGLSTFNINENFRSLSLQRKGRANFSNKIATIQAASDEIIVI
                     KYSDGQVCLLYRENGIATLATENATFAEYLIFSTNVPKSGGYGIEVYNSAGNVVFSSN
                     HKFLRPIEYVDLNPSKNVFRFTGQANRQYGIILSNFGFDFVINYDNGYSTSRSAKVSG
                     NFIEFGFCKYHYSGLYKTGMSYNSKAMYFNALVVDITGY"
     gene            1348..1641
                     /locus_tag="PWY43_000961"
     CDS             1348..1641
                     /locus_tag="PWY43_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135854.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGEESDGAVTK
                     FVTITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYNHLKETGQIVV"
     gene            complement(1926..2768)
                     /locus_tag="PWY43_000962"
     CDS             complement(1926..2768)
                     /locus_tag="PWY43_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HDL2135855.1"
                     /translation="MIKVHCLTTGWVQIKIHHQVARFFAHPARVLDVLTDKKWSPKLP
                     IGCWLIEHDDGLILVDTGESSSANDKGYQPWWHPFMQFCERRGVKKHEEVGEILKANG
                     FDPLRIDTVVMTHMHGDHAGGLPNFPNSRFVMSETEKQAINAPNAVMNGYLTMHYPDW
                     FNPCGIAFNDGAFESFEQSHKLTKDGKIRLVPTAGHTLGHLAVVVDMGEYDILIGGDA
                     SYSEQDMLAGNIDGVCVDGHLHQQSTAKMRQLCQRKPTITQFAHDEKSEYRLKNKVFT
                     VVKN"
     gene            2899..3489
                     /locus_tag="PWY43_000963"
     CDS             2899..3489
                     /locus_tag="PWY43_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2135856.1"
                     /translation="MNDLRVIKTHLAIQNALIELLDEKPFEKVQVQEIIEKALINRTT
                     FYRYYSGKSDLVGKLIKEVKAEYQDLLKKRFESDDLLRFMQTIGNRLLEKRRLILALW
                     QIKTPRHHLYDDMHTLLKNAFIAHAKRITSDDKNWADKNWAEKNWAEKNWDYQAHIFA
                     TIALESHKYYFEQGKLLPIPQVFEAWIEMVQVLKSV"
     gene            complement(3555..4433)
                     /locus_tag="PWY43_000964"
     CDS             complement(3555..4433)
                     /locus_tag="PWY43_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2135857.1"
                     /translation="MSKQIQFTKTGSPDVLQIVDVQIPAPKAFEVQIQIQAIGLNRAE
                     MMYREGAYVIDPVFPATLGYEGAGVVVAIGEGVNELAIGDKVSIIPSFMFTEYGTYGE
                     IVNMPKHAVVKHPDNLSMEQASASWMAFVTAYGGLIEFDKVQKGDVLLEKGADFVIAT
                     SEDDVTAKLFEITNGKGVNVVFDPVGGKEAAKIINAMAQDGRYIIYGALSHDDIAVPV
                     FPILGKHLTVRGYELFEITTVPEKLAQAKTFVYDGLASGQLRPEIDKIFAFDEMVKAH
                     EYMASNQQIGKIVVKV"
     gene            complement(4647..5564)
                     /locus_tag="PWY43_000965"
     CDS             complement(4647..5564)
                     /locus_tag="PWY43_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL2135858.1"
                     /translation="MTFIIGILAGVLIAFQSAINARLRMHVGAAFLTSFVSFSVGLLF
                     LLGLSLIMQQTPAFSLDNLGNVPLWAWTGGLLGMVGLTANVLIFPKLGGVQTAVMPIL
                     GQVLMGVLIDTFAWLNSPYIAFTASRLFGIGLVLFGVWIAVVLPHLSGLKRKENANLW
                     LWRAVGILGGTTLATQAAVNGEFGRQLGSSLGAATVSFGVGAIGLLLVVLFYEKSLPK
                     LCQPTEGKPFWIWSGSILGSLFILAGVWLVPQIGTGSVVMLVLSGLICGSLLVDKFGL
                     FGITQKPILPLQIIGVTLLLAGVAFIRLL"
     gene            complement(5700..6359)
                     /locus_tag="PWY43_000966"
     CDS             complement(5700..6359)
                     /locus_tag="PWY43_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2135859.1"
                     /translation="MQFFNKEDVINAFHYRASTRSYDGSRKIPAEDFNYILELGRLSP
                     SSVGSEPWQFLVLQNPELRQKIKPYCWGIPTMETSSHIVVILAKKNARYDSQYFSEIM
                     DRRGLQGEAREKAMAVYKKFQEKDIGILDSERSLFDWASKQTYIALGNMMTGAALIGV
                     DSCPIEGFAYQEVNDILAAEGLFDPSEWDVSVMCTFGYRDKEIRQKARKDFETVVKFI
                     E"
     gene            complement(6544..7608)
                     /locus_tag="PWY43_000967"
     CDS             complement(6544..7608)
                     /locus_tag="PWY43_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HDL2135860.1"
                     /translation="MAKFRKLFTPFNIKNLALKNRVVMPPMCQYSATNGVPNDWHFVH
                     YTSRAVGGVGLSIVEMTNVAPNGRITPNCLGLWNDEQQQAFKKLVDSVHAQGAKIGVQ
                     IAHAGRKTQDEPDAVAPSAIHYGELDYAGQNLITPRELTTDEVKALVQAFKDSVKRAV
                     EAGFDTIEIHGAHGYLIHQFYSPKSNQRSDEYGQDKMLFGEQVIQAAREVMPSEMPLI
                     VRISAQEYGENGFGLDYGLQVAKRFADAGADVLDISGGGDGVLHAGNHPEFYAGYQVD
                     FARQVKLATGKPVIAVGMLDNPAVGDHILGMGDADLVAVGRALLRDPYWVLNAQYDQN
                     AADSSEMQFVPKQYERGLFM"
     gene            7699..8447
                     /locus_tag="PWY43_000968"
                     /pseudo
     CDS             7699..8447
                     /locus_tag="PWY43_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248535.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(8517..9068)
                     /locus_tag="PWY43_000969"
     CDS             complement(8517..9068)
                     /locus_tag="PWY43_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2135861.1"
                     /translation="MAKQIAVLIGSGSKTSFSHLTVRHLQKMAPASIQLNIVDIADLP
                     LYDRDLDENSPAQYTRVREAVAVADGVLLVTPEHNASFSAMLKNAIDVVSRPMGQSKW
                     IGKPAGIVTVGASPAGGVRAADQVRTITSGSFINMPTLPFALNVGNLFAGVFDEQGEI
                     ASEALKSAMQNFINAYAQFVEKY"
     gene            9260..10621
                     /locus_tag="PWY43_000970"
     CDS             9260..10621
                     /locus_tag="PWY43_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL2135862.1"
                     /translation="MLFLNKIKQLFIFAFTLVLSACSLVVYKPVETIKQVNVERGYRQ
                     KNVIAESAKDGNIVVIMFSGGGTRAAALGYGVLEEFKKVRVKETNKGNTLLENIDIAF
                     GVSGGSILATYFALEGKDIIPKFADRFLKANFQKDIITEVFSLSNLPRLSSPQYGRGD
                     LLQERLNFALYHGKKFGDLVKNRKGPFAVISATDMNIGQKLTFTQETFDGLCLNLNDL
                     EISRAVAASSSVPLIFAPLTLNNNGGNCHFSMPQEFSAREKAIDGLITKNIEETNHIL
                     SLYQDSKERPFIHLVDGGLTDNLGLASVLEISDVMGIEEMYLYIHQLGVKNIVVINVN
                     AQNEVSSEIDKTANVPGVADVVNTIVNVPIDRTTQINLQRYRQFTDGWNEYANKQPSK
                     IEMHFVSLGLKELPDGDLKRDVLNIATSFYLPEDDVDKLREAARVLLQQSQEYNAALK
                     ILQ"
     gene            complement(10669..11166)
                     /gene="rraA"
                     /locus_tag="PWY43_000971"
     CDS             complement(10669..11166)
                     /gene="rraA"
                     /locus_tag="PWY43_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDL2135863.1"
                     /translation="MRIDTSALCDLYSDQVDVVEPIFSSFGGASSFFGKITTVKCFES
                     NGLIADVLEEEGEGRVLLVDGGGAVRRALIDAELAQLAVDNGWEGIIVYGAVRQLDIL
                     ERLDIGIHALAPIPVGADNTDIGEVDTPVNFGGVTFFPEDYIYADLTGIILSQELLDL
                     SDISE"
     gene            complement(11264..12167)
                     /locus_tag="PWY43_000972"
                     /pseudo
     CDS             complement(11264..12167)
                     /locus_tag="PWY43_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
     gene            12276..13238
                     /gene="rluC"
                     /locus_tag="PWY43_000973"
     CDS             12276..13238
                     /gene="rluC"
                     /locus_tag="PWY43_000973"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDL2135864.1"
                     /translation="MTTEKVISAGVQFFTISEDEAGQRLDNFLLAKLKGVPKSLIYRI
                     VRKGEVRVNKGRIKPEYKLQPDDLVRVPPVRVAEKNEAPISTKLNKVAELESQILYED
                     DSMLVINKPSGIAVHGGSGLSFGVIEALRALRPQARFLELVHRIDRDTSGILLVAKKR
                     SALRSLHEQLREKVVQKDYLALVRGQWQAHTKVIKAPLLKNELASGERIVRVSEEGKP
                     SETRFSIEERYANATLVKASPITGRTHQIRVHTQYAGHPIALDDKYGDVEFDSKMKEV
                     GLQRLFLHAHAIRFEHPKTGEEMVITAPLDKTLKGVLAKLRANY"
     gene            13347..14204
                     /gene="folP"
                     /locus_tag="PWY43_000974"
     CDS             13347..14204
                     /gene="folP"
                     /locus_tag="PWY43_000974"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDL2135865.1"
                     /translation="MQIHFKNFQKNDRLLDLTTPQIMGILNFTPDSFSDSGKFFSLDK
                     ALFQAEKMLADGASIIDIGGESTRPNAAIVSLEQELERVVPVVEAIRSRFDCLISVDS
                     SKAEVFKESAKVGMDIINDIRALTEPNALAAAVELGLPVCLMHMQGNPQTMQQNPHYD
                     DVLEDVADFLNQRIFECITAGIPKEHIILDPGFGFGKSVKHNYQLLKHLKAFVDSGFP
                     VLAGLSRKSMIGAVLDKPVEQRLLGSVVGALLAAQNGAKILRVHDVAETADALKIWQA
                     MLESDNEEK"
     gene            14204..14551
                     /locus_tag="PWY43_000975"
     CDS             14204..14551
                     /locus_tag="PWY43_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDL2135866.1"
                     /translation="MTTLVYGIKNCDTVKKALKWLEDHNQNPQLHDYRVDGLNADWLV
                     EMEAKFGWENLVNKRSTTWRNLDEEIKNNLNRESALKVLTEQPTLIKRPIVIKEDIAL
                     IGFNEKEYVAAFN"
     gene            14681..15829
                     /gene="dapE"
                     /locus_tag="PWY43_000976"
     CDS             14681..15829
                     /gene="dapE"
                     /locus_tag="PWY43_000976"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDL2135867.1"
                     /translation="MKNEIISLAQNLIRRDSISPQDKGCQQEIAQRLEKRGFNIEWLP
                     FGDTLNLWATHGSENGKTEPCIVFAGHTDVVPVGDESQWAYPPFSAEIVDGMLYGRGA
                     ADMKGSLSALVVAAETFVQHHPNHKGKVALLITSDEEAAAKDGTVKVVETLMARNEAV
                     HYAVVGEPSSSKVFGDVIKNGRRGSITADLYIEGIQGHVAYPHLAENPVHTSLGFLTD
                     LTTYQWDNGNEFFPPTSLQIANIKAGTGSNNVIPGELYIQFNLRYCTEVTDEIIKAKV
                     TEMLEKQGLKYRISWNLSGKPFLAGNGKLVEAALQAVENVAKITPRLDTGGGTSDGRF
                     IALMGAEVVEFGPLNTTIHKVNECVSVDDLANCGEIYYQILEKLLANA"
     gene            15940..17274
                     /locus_tag="PWY43_000977"
     CDS             15940..17274
                     /locus_tag="PWY43_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL2135868.1"
                     /translation="MQLYSYICLLFGLAMFISFFTRKLNENIQTTIAITASALVGSLL
                     ILTFGSLGWFHVDKLAISIFEQLDFKSFLLNGMLGFLLFAGSLGIKLPLMKEQRREIA
                     VYALLSTLISTFLIGALIYGVAKLCGLEIGFVYCLLFGSLISPTDPIAVLAIIKSLKA
                     PKRLSMHVEGESLFNDGIGLVIFTTIFAVAFGGQAPTLSGITGLFLQEAVGGIVFGLL
                     TGFVAHKFISATDDGSLEILITLTIPTVGFVIANYLHVSGALTMVVAGIMIGNWTRRS
                     GFSEQSQKYLDHFWEMIDHSLNYLLFLLIGLSTLLVDFTFIGGVLMLAAIPICLLARY
                     ISLWIPYQVLKRFRTYNPYTLRIMTWGGLRGGLALAMALSVPAGAAMVSSSEGELDVR
                     DLILLMTYAVVTFSILVQGSTVEPMIKKSKLVDPRRVALNKLGGPDDLIPHI"
     gene            17383..17979
                     /locus_tag="PWY43_000978"
     CDS             17383..17979
                     /locus_tag="PWY43_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL2135869.1"
                     /translation="MLITGLLCGFLLGFVMQRGRFCITGAFRDLYVTKNSRMFVAFLI
                     AITVQSIGIWLLYEAGSFSSPAEDLPLLAVIIGAFLFGIGIVYAGGCATGTWYRAGEG
                     LIGSWVALIVYGLFSASMRTGVLAPLNQELKSNVIEHRTIYETFGISPWVLVFILSVI
                     TFALTFYHLRKPRGKTITLKPRKTGLAHILFEKRWHPF"
     gene            17976..18410
                     /locus_tag="PWY43_000979"
     CDS             17976..18410
                     /locus_tag="PWY43_000979"
                     /inference="COORDINATES: protein motif:HMM:NF016064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL2135870.1"
                     /translation="MTAVLVGLIALLAWPLSSAAGRNFGLGITTPSANLVQYFVTGEV
                     KFINWAVFLVLGILIGSFVAAKFSNEFRFRVPDATTMLRSAGGGALMGIGASLAGGCS
                     IGNGLVETAFFSWQGWLSLPIMILGTFVGAYFTIIRPQRLKG"
     gene            18432..18653
                     /locus_tag="PWY43_000980"
     CDS             18432..18653
                     /locus_tag="PWY43_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL2135871.1"
                     /translation="MNVRLPASGLVCPFPLVEAKAAMAKLNKGDSLTIEFDCTQATEA
                     IPNWAEEEGYEVTDYQQLGDALWEITVVK"
     gene            complement(18716..20224)
                     /locus_tag="PWY43_000981"
     CDS             complement(18716..20224)
                     /locus_tag="PWY43_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDL2135872.1"
                     /translation="MSKCPVTHLTMNNGAPVVDNQNSLTAGPRGPLLAQDLWLNEKLA
                     NFVREVIPERRMHAKGSGAFGTFTVTNDITKYTRAAIFSQVGKKTEMFARFTTVAGER
                     GAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNMRS
                     ATNNWDFWTLLPEAFHQVTIVMSERGIPASYRHMHGYGSHTYSFINAANERFWVKFHF
                     HTQQGIKNLTNEEAAAIIANDRESHQRDLYEAIERGDFPKWKMFVQIMPETDADKVPY
                     HPFDLTKVWPKSDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGISVSPDRMLQ
                     ARLFNYADAQRYRLGVNHHQIPVNAPRCPVHSNARDGQGRVDGNYGSTIHYEPNSFGK
                     WQEQAQYAEPPLKINGDAAHWNYREDDADYFSQPRVLFNMMNDEQKQALFNNTAAGMG
                     DALDFIKYRHIRNCYACDPAYGEGVAKALGMTVEDAIAARATDPALGQPGLL"
BASE COUNT         5955 a         4147 c         4449 g         6017 t
ORIGIN      
        1 gcaatatcgg caaatctcgg agctattaca gctggttcta ttaacatcaa taatagattc
       61 aaggtaagta atgctggagt agtcgaaatg cgagccaata gtggaaatgt tggtatggta
      121 atgaataacg atagtattat tgtttatgat gaacaaggta gggttagagt aaaaatgggg
      181 aaattatgat tctaataggt attttatgta ttattgcatt agcagtctta gggctgcttt
      241 tttatttcca taaaaaaaaa cgaaaaaaag taaataaagg ggtaaatatg gctgaatatg
      301 gtattcaaac ttttgatacc gctaatgtct ctacatttag taccaatgag cgattattta
      361 aatacattgg aactaaaaat attaagtatg gtaaattttc attcacggta acagaaaagt
      421 atgaaggaca acttgtattt atcattagta tgttacattc agttcaccga aaacaagcaa
      481 caggttataa aacgaatatt cccaatctta ttcatttaga aaatgtttcg atcaacgata
      541 aaacgatttc aggagaagtt gtttgtattt ttccttttag tctaccggct gatgattcag
      601 attatattca gattgtatat ggagcatatt aaatgagtta cggactatct acttttaaca
      661 tcaatgaaaa tttcagaagt ttatccttac aaagaaaagg acgagctaac ttttctaata
      721 aaattgcaac tattcaggca gcgtcagatg aaattattgt cattaaatat tctgatgggc
      781 aagtatgttt gctatatcga gaaaatggta ttgccacatt agctacagaa aacgcaacat
      841 ttgctgaata tctcattttt agtacaaatg taccgaaaag cggaggttac ggcatagagg
      901 tttataactc agcaggaaat gttgtatttt catcaaacca caaattttta agacctattg
      961 aatatgtcga tctaaatcca tcaaaaaatg tttttagatt tacagggcaa gcaaataggc
     1021 aatatggcat tatcttatcc aattttggct ttgattttgt cattaactac gataacggat
     1081 attcaacatc tagatctgcc aaagtaagtg gtaattttat tgaatttggg ttctgtaaat
     1141 accattattc tggtttgtat aagacgggaa tgagttataa ttcaaaagca atgtatttta
     1201 atgctctggt tgtagatata acaggctatt aaacattaaa cgccttcatt taatagaggc
     1261 gaactaaaat tctgattaca cattcatcgc cgcttgtagc aatacaagcg gtcttttttt
     1321 atcaaaaatc cacaaaagga aaaccttatg acaaccttta acaaaatctt aaacccgatg
     1381 tactcaacta tcgcaagtta ctcaactcaa gatgacggct cactcaatgc taaatatgtc
     1441 gttggcacag gtgaggagag cgatggtgct gtgaccaaat ttgtgacaat taccagcgag
     1501 tacaaataca ttgatgcaca gtccgccaag gcgattactg atgcaccgct aacaaaagag
     1561 gatattggca aaacgccaac gcagattatg ttaggtcgta tttacaatca cttaaaagag
     1621 acggggcaga ttgtagtgta atagttctta tagattagaa ccgcttaggg aaaccaagcg
     1681 gtttttttgt actaggaggc acaatgttta cagcaacgct ttccattgat aatccgtatg
     1741 ttgcaaaata agcattaaaa aacgttccga aacaaatcgc atttatttga taaataaagg
     1801 aattttttaa tgaaattcaa tgttttttcg gaacgtttta tttataataa gccttatgct
     1861 cacttaagtt tgaaatattt ttaataaaaa tggccgcttg cctttcaaca agcggtcatt
     1921 cccccttaat tttttaccac cgtaaacacc ttatttttca ggcgatattc actcttttca
     1981 tcgtgagcaa attgggtaat ggtcggtttt ctttggcaaa gctgacgcat tttggcggtg
     2041 ctttgttggt gcagatgtcc gtcaacgcac acgccgtcaa tgttgccagc aagcatatct
     2101 tgttcagagt aagaggcatc tccgccaatc aaaatatcat actcgcccat atccaccacc
     2161 accgccaaat gccccagcgt atgtcctgcg gttggcacaa ggcggatttt gccgtctttg
     2221 gtgagtttat gactttgttc aaaactttca aacgccccat cattaaaggc gataccgcaa
     2281 ggattaaacc aatcaggata gtgcattgtc aaataaccgt tcatcaccgc atttggggcg
     2341 ttgattgcct gtttttcagt ctcactcatc acaaatcggc tgtttgggaa attgggcaat
     2401 ccgcctgcgt ggtcgccgtg catatgggtc attaccaccg tgtcaatgcg taaagggtca
     2461 aagccgtttg cctttaaaat ttcgcccact tcttcgtgtt ttttgacccc acgccgttcg
     2521 caaaactgca taaatggatg ccaccacggt tgataaccct tgtcgttggc ggaagaactt
     2581 tcgcctgtat ccaccaaaat caaaccgtca tcgtgttcaa tcagccaaca gccaatcggc
     2641 agtttgggcg accatttttt atcggtcagc acatcaagca cacgagcagg atgggcaaaa
     2701 aagcgtgcca cttgatgatg aattttgatt tgtacccaac ctgttgtcag acaatgtact
     2761 ttaatcattt tgtgttctca ttaaaaaatc gtgggcatat tgtagaaagt gcggtcactt
     2821 ttcacaaaca ttaaaaagct cgtatttagc aggatatgca gataaaatta taaaaatgta
     2881 tgcttatttt gaaaaaagat gaacgattta agagtaatca aaacccatct ggcaatccaa
     2941 aacgctttga ttgagttatt ggatgaaaag ccgtttgaaa aagtgcaagt ccaagaaatc
     3001 attgaaaaag ccttgattaa ccgcaccact ttttaccgtt attattcggg caaaagtgat
     3061 ttggtcggca aattgataaa agaggtgaag gcggaatatc aagacctgct caaaaagcgt
     3121 tttgaaagcg atgatttact gcgatttatg caaaccattg gcaaccgctt attggaaaaa
     3181 agacggctga ttttggcact ttggcaaatc aaaaccccac gccatcattt gtatgacgat
     3241 atgcacacgc tgttaaaaaa cgcctttatc gcccacgcca aacggataac atcagatgat
     3301 aaaaattggg ctgataaaaa ctgggctgag aaaaattggg ctgagaaaaa ttgggattat
     3361 caagcccata tttttgccac catcgccctt gaaagccata aatattattt tgaacaaggc
     3421 aaacttttgc ccattccaca ggtgtttgag gcgtggatag aaatggtgca ggtgttgaaa
     3481 agtgtttaga aataactggt aaaattttcc ataaaaaaca ccgctttcat cacaaaagcg
     3541 gtgctttcaa atcatcaaac cttaaccaca atcttaccaa tctgctgatt gcttgccata
     3601 tattcgtgag ctttaaccat ttcatcaaag gcaaagattt tgtcaatttc agggcgtaac
     3661 tgaccgcttg ccaaaccgtc atagacaaag gttttggctt gtgccaattt ttcaggtacg
     3721 gtggtaattt caaagagttc atacccccgc acggtcaaat gcttacccaa aatcgggaac
     3781 actggcacag caatatcatc atggcttaac gctccataaa tgatgtaacg accatcttgt
     3841 gccattgcat tgatgatttt tgccgcctct tttccgccca cagggtcaaa taccacattg
     3901 acgcctttgc cattggtgat ttcaaaaagt ttggcggtta catcgtcttc gcttgtggca
     3961 atcacaaaat ccgctccttt ttccaaaagt acatcgcctt tttgtacttt gtcaaactca
     4021 atcagtccgc cataagcggt aacaaatgcc atccaactgg cggacgcttg ttccattgat
     4081 aaattatcag ggtgcttaac gacggcgtgt ttgggcatat tgacgatttc gccataagtg
     4141 ccgtattcgg taaacataaa agatggaata atgctgacct tatcgccaat ggcaagctcg
     4201 ttcacaccct cgccaatcgc cacgaccacg cctgcccctt cataacccaa agtcgcaggg
     4261 aatacagggt caatcacata cgccccttca cggtacatca tttcggcacg gtttaagcca
     4321 atggcttgga tttggatttg tacctcaaac gctttggggg cggggatttg tacatcaacg
     4381 atttgcaaga catcaggcga acctgttttg gtaaattgga tttgtttgct cattgttttg
     4441 accttttgtg aaaataatgg gcgtatttta atgcaatgaa tttattttaa aaatcacatc
     4501 aatcgcaaaa cacttttgct aaatttacat aaatacattt tcacaatgtc tgaaaagttg
     4561 ttttgcaaat tttaaaaaga aaaccaccgc ttgccgatca caagcggtgg tttttttggg
     4621 ttattttgca agaggtgtca gcttggctac agtaagcgga taaatgcgac gccggctaag
     4681 agtaaggtaa cgccgatgat ctgcagcggt agaatgggtt tttgtgtaat gccgaacaga
     4741 ccgaatttat ctaccagcag gctgccgcaa atcaggccag agaggacgag catcaccacc
     4801 gagcctgtgc cgatttgggg gacgagccaa acgccagcga ggataaacag gctgcccagt
     4861 atgctgccgc tccatatcca aaacggtttg ccttcggttg gctggcagag tttcggcagg
     4921 cttttttcat aaaacagcac taccagtaat aagccgatgg caccgacacc gaaggagact
     4981 gttgctgcgc cgagtgatga gccgagctgt ctgccgaatt cgccattaac ggcggcttgg
     5041 gttgctaatg tggttccgcc taatatgccg actgcgcgcc acagccataa atttgcgttt
     5101 tctttgcgtt ttagaccgga cagatggggc agtaccacgg caatccatac gccgaataag
     5161 actaaaccaa tgccaaacag gcggctggcc gtgaaggcta tgtaaggcga gttaagccaa
     5221 gcgaaagtgt cgatcagcac gcccatcagc acttggccta aaatcggcat gaccgcagtt
     5281 tgtacgccgc cgagcttggg gaagatcagt acgtttgccg ttagccccac catgccgagt
     5341 aatccgcccg tccatgccca taaggggacg ttgcctaaat tgtcgaggct aaatgctggt
     5401 gtttgttgca tgatgagcga gagtccgagt agaaacagga ggcctacgct gaaagagaca
     5461 aacgaggtta aaaatgccgc acccacgtgc atacgcaaac gggcgttgat ggctgattga
     5521 aatgcaatca gcacgccggc taaaataccg ataataaatg tcatgactaa ctgggttgtg
     5581 ttgccgcctc aaaatgagtg gtggttgaaa agaaatgccg tctgaatgcg gattcagacg
     5641 gccttaaggc ttggtatcgg cgtttacctg atgttgaata gccgttttgc ctagccggtt
     5701 tattcgatga atttcaccac ggtttcgaaa tctttacgtg ctttttggcg gatttcttta
     5761 tcacggtaac cgaaggtaca catcaccgat acgtcccatt cgcttgggtc aaataatcct
     5821 tccgctgcca aaatatcgtt gacttcttgg taagcgaaac cttctatcgg acaagaatct
     5881 acgccaatta aagctgcacc ggtcatcata ttgcccaatg cgatgtaggt ttgtttgctt
     5941 gcccagtcaa acaggctgcg ttcgctgtct aaaatgccga tatctttctc ttggaatttt
     6001 ttatacacgg ccatggcttt ttcgcgcgct tcgccttgca aacctcgtct atccataatt
     6061 tcagaaaaat attggctgtc gtaacgggca tttttcttcg ctaaaatcac cacgatatgg
     6121 cttgaggttt ccatagttgg aatgccccag cagtaaggtt tgattttttg ccgtaattca
     6181 gggttttgca acactaaaaa ctgccaaggc tctgagccta ccgaacttgg acttaaacga
     6241 ccgagttcca gaatatagtt gaaatcttca gccggaattt ttctgctgcc gtcgtaagaa
     6301 cgggtagatg cacggtaatg aaaggcattg attacgtctt ctttgttgaa aaattgcatg
     6361 gaacactcct aaaaatgcaa aacagtcttt tcagacggtc tgaaaagact agatgaaaga
     6421 cggataagta taaaagattt aagttatttt taataattga taattttgat atttaggtat
     6481 aagttgacgt gatgagaaaa aacagaccct ctgaatgttc agacggtctt taacgttggt
     6541 tgtttacata aataatccgc gttcatattg cttcggtaca aactgcattt cactgctgtc
     6601 tgctgcattt tggtcatatt gcgcattcaa tacccagtac ggatcgcgca ataaggctct
     6661 tcccacagcc actaaatccg catcgcccat acctaagata tgatcgccta ccgccggatt
     6721 atccaacata cctactgcaa ttaccggttt gcccgtagcg agcttcactt gacgggcaaa
     6781 gtctacttgg taacctgcgt aaaattcggg gtgattacct gcatgcaata caccatcacc
     6841 accaccgctg atgtctaaga catctgcgcc tgcatcggca aaacgtttcg ctacctgcaa
     6901 accataatcc aagccaaagc cgttttctcc gtattcttgt gcagaaatgc ggacaattaa
     6961 cggcatttca gacggcatca cttcacgtgc tgcctgaatc acttgctcgc caaacaacat
     7021 tttgtcttgg ccgtactcat cactgcgctg attggatttc ggcgaataga attggtgaat
     7081 tagataaccg tgtgcgccgt ggatttcaat tgtatcaaag cctgcctcaa cggcacgttt
     7141 gaccgaatcc ttaaaggctt gaaccaatgc tttgacttcg tctgtcgtca gctcgcgagg
     7201 tgtaatcaga ttttgtccgg cataatccaa ctcaccgtag tgaattgcac ttggagccac
     7261 tgcatccggc tcgtcttggg ttttgcgtcc ggcatgggcg atttgcacac cgatttttgc
     7321 cccttgagca tgaacgctgt ctaccagttt tttgaatgcc tgttgttgtt catcgttcca
     7381 taagcctaag cagtttggcg taattctgcc gtttggcgcc acattggtca tctccacaat
     7441 actcaagccg acaccaccta ctgcacggct ggtgtaatgc acgaaatgcc aatcgttcgg
     7501 tacgccgttg gttgccgaat attgacacat tggtggcata accacacggt ttttcaatgc
     7561 taagttttta atgttaaacg gggtgaataa ttttctgaac ttcgccatgt tgccttcctt
     7621 aatgaaatag attaggtgga atgataattc agtgctatta tctattcaaa ttgattattg
     7681 tgatagttag gtataaaaat gagtgatata agaacactgg atttcaattt gctcaaagct
     7741 tttgtggtgc tgctggacga atgcaacgtt agccgggcag cacaacgatt atccatcacc
     7801 caaccggcaa tgagcggtat tttaaaccgc ttgcgcgaga gttttaacga tccgttattt
     7861 gtgcgagtgc aacacggtat gcagccaacc gaccgcgcgt tgcaactggg gcaaaccgca
     7921 cgcaacattc tgcaagatat cagctccatg ctgcaaccac ccatattgga accggaacat
     7981 ctgaatatga ccttgcgtat tgccgcaatg gattatgtac aacaaattat tgccttgccg
     8041 ctgattctgc gtttaagacg gcttgctccg aatgtaaaag tcgccttgct gccagtgcag
     8101 gggcaaaata tcaaaacctt gttcgagcaa aacaaaatcg atcttgcctt ggtcagccgc
     8161 caacattgga tctagcgtta caaaagatag gactacagcg caaagtaatg gtctcggtaa
     8221 accatatttc cctgctgccc cagctgctac aaggttccga cttagtcgca gtattgcccg
     8281 aacgcttagc aaagacctta cccaatgttt atttacaacc tccgcctata gaggtagaag
     8341 gcttcaccat gatgatggct tggcacgaaa gaaccgaaca agatacggca catcggtggt
     8401 taagggaggt actgcagggt gtggtaaaac ctaaacaggc tctttaatta tcctttcttt
     8461 tactacgaaa aaagccgttt atatttttag ataaacggct tttttgcatt ataagattag
     8521 tatttttcta caaactgagc ataggcatta atgaagtttt gcattgcact ttttaatgcc
     8581 tcagaggcaa tttcaccctg ctcatcaaat acgcctgcaa ataagttacc tacgtttaat
     8641 gcaaaaggta aagttggcat attgataaat gagcctgaag taatagtacg cacttgatca
     8701 gcggcacgca caccacctgc tgggcttgca ccaacggtta cgatacctgc aggtttgcca
     8761 atccatttgc tttgccccat tgggcgagaa accacgtcaa tcgcattttt aagcattgca
     8821 gaaaagcttg cattatgctc aggtgtgact aataacacgc catctgccac agcaaccgct
     8881 tcacgcactc gggtgtattg tgccgggctg ttttcatcta aatcacggtc gtaaagtggt
     8941 aagtctgcaa tatccacaat gtttaattga atgcttgcag gtgccatttt ttgtaagtga
     9001 cgaacagtca agtgactaaa tgaagttttg ctgccgctac caattaatac tgcaatttgt
     9061 tttgccattt tataattcct tagtacatag agaatatagg cattttatcc ctttcaatct
     9121 gtttcataaa gcgcttatga tgaaaagcac tatttactta tattaaataa ttttgcaaat
     9181 tttaggccaa atttgaccgc atttatcttt agctaaaaag gttataattt gtagaaatag
     9241 atcttaattt agggttttta tgctttttct aaacaaaata aaacagcttt tcatcttcgc
     9301 ctttacgcta gtgttatctg cttgcagttt ggttgtttat aagccagttg aaacaattaa
     9361 acaggtaaat gttgaaagag gttatcgaca aaaaaatgtg atcgctgaaa gtgcaaaaga
     9421 cggtaatatt gttgttatca tgttttcagg tggtggaaca agagcagcgg cgcttggtta
     9481 tggagtgttg gaagagttta aaaaggtaag ggtgaaagaa acaaataaag gtaatacttt
     9541 attagagaat atagatattg cctttggcgt atcaggtgga tcgatattag ccacttattt
     9601 tgcgttggaa ggaaaggata tcattcctaa atttgctgat cgttttttaa aagcaaactt
     9661 tcaaaaagat attattacag aggtgttttc gttatccaat cttcctcgtt taagttctcc
     9721 tcaatatggg cgtggtgatt tattgcaaga acgcttaaat tttgcccttt atcatggtaa
     9781 aaagtttggt gatttagtga aaaatcgtaa aggacctttt gctgtgatta gcgcaacaga
     9841 catgaatatt ggtcaaaaac tgacctttac gcaagaaacc tttgatgggc tttgtttaaa
     9901 tttgaatgat ttagaaattt cccgtgctgt tgctgcttct agttctgttc ctctgatttt
     9961 tgcgccgctt acactgaata acaatggtgg aaattgccat tttagtatgc cacaagaatt
    10021 tagtgctagg gaaaaagcaa ttgatggact aattacaaaa aatattgaag aaacgaatca
    10081 tatactcagc ctttatcaag atagtaaaga gcgccccttt atccatttag ttgatggtgg
    10141 attaacggat aatttagggc ttgccagtgt gctggaaata tctgatgtta tgggtattga
    10201 agagatgtac ctgtatattc atcagcttgg ggtgaaaaat attgtagtga ttaatgtaaa
    10261 tgcgcaaaat gaagtctcta gcgagatcga taagaccgca aatgtgccgg gtgtggctga
    10321 tgtagtgaat acaatagtta acgtgccaat tgatagaacg acacaaatta atttacagcg
    10381 ttatcgccag tttacggatg gttggaatga atatgcaaat aaacagccat cgaagatcga
    10441 aatgcatttt gttagtttag gtttaaaaga attacctgat ggtgatttaa aaagagatgt
    10501 gctaaatatt gcaacatcat tttatctgcc ggaagacgat gtagataaat tacgtgaagc
    10561 cgctcgagtt ttattacagc aatctcaaga atataatgcg gcattaaaaa tattgcagta
    10621 gtaaaaaacc gaccaagcgg tcggtttttg gtattttttt gcaaatattt attcgctaat
    10681 atcgcttaaa tccaacaact cttgcgaaag aataatgccg gttaaatcag cgtaaatgta
    10741 atcttcaggg aagaacgtta cgccaccgaa attgaccggt gtgtccactt ctccaatatc
    10801 ggtgttatct gctcccactg gaattggcgc taaagcatga ataccgatat ctaatctttc
    10861 caaaatatct aattggcgaa ccgcaccata aacgatgata ccttcccagc cgttatctac
    10921 agcaagttga gccagctctg cgtcaattaa ggcacgacga actgcaccgc caccatcgac
    10981 taacagcact cgcccttcac cctcttcttc caaaacatca gcaattaaac cgttactttc
    11041 aaagcattta accgtggtaa tcttcccaaa gaaggaagaa gcaccgccaa agcttgagaa
    11101 aattggttca accacatcaa cttggtcaga ataaagatcg caaagtgcag aggtatcaat
    11161 acgcataata tgctcctgtt cgtagattat ttagctaaag tataaacctt cgcccaaaat
    11221 ttgcaaaatt ttatcgaaaa aagaccgctt acaggctttt ttcttatgct ttattctcta
    11281 gtgaaccgct tttaatatgc aattccattt ggttgtaagg aatagatata cctgcttcac
    11341 caaaggctaa ttttacttgt tcgataattt cccaatatgc tgcccagtaa tttggcgtct
    11401 gaacccaagg gcgaacaaca agttgaacac tatgcggtgc taaagcgccg actgctacta
    11461 caggtgccgg atcttttaat acataagtat tggcgtctaa aatgcgttgg ataatctctt
    11521 tcgcctcttt taaagttaga ttcataagaa atatcaaagg tgaaatcaat acggcgtgtc
    11581 gggttgttag agtagttgat gatgctgtca gaaaacactt tgccgtttgg taataacacg
    11641 gttttgttat cgccggtgcg aagttccaac actaaaagcc ccatttgttc agccacgccg
    11701 gtcattccgc cggtttcgat taaatcccct ttgcgaaatg gcttgaagat taacaacatc
    11761 acgccggcag caaagttttg taatgagttt tgtaatgata aaccgatggc taaaccggca
    11821 gcaccgatta atgcaaccag cgagctggtg ttaatgccga tttgtgaaag cgaggcaatc
    11881 accacaatta acagcagtaa aaaatagctg attgagacaa taaaactttg cagcatttca
    11941 tctttggttg attttaatgc cgctttgccg agtaaacggc taatcaggcg ggcaagaaat
    12001 ttcccgacta caaaaatcgc aatcgctagt aaaattttgg tgccataagg gataatccac
    12061 tcgtttaata cggaattaaa atccattgtg ctgactttgt taattacttt ttcggtttcg
    12121 gcaaccacgt caatatttgg ggcggtttcg gtgacttttt cagtcatatt gtttccttaa
    12181 aattaaaatg aaaaataagc ctagcgaatt tgcctcactc gctaagcggt tttgtgctag
    12241 aatttagagc aaaattttct ctaaaagaca agataatgac aacagaaaaa gtaattagtg
    12301 ccggtgttca attttttacg atcagtgaag atgaagccgg acagcgttta gataattttt
    12361 tattggcaaa gctcaaaggc gtgccgaaaa gtttgattta ccgcattgtg cgaaaaggcg
    12421 aggtgcgggt gaataaaggg cgcataaagc ccgaatataa attacagccc gacgatttag
    12481 tgcgtgtgcc accggtgcgt gtagcggaaa aaaatgaagc cccaatttcg actaaattaa
    12541 acaaagtggc ggagctggaa agccaaattc tgtatgaaga tgactcaatg ctagtaatta
    12601 acaaaccatc aggtattgct gtacacggtg gcagtggttt aagttttggt gtgattgagg
    12661 ctttgcgtgc gttacgtcca caggctcgtt tcttggaatt ggtacaccgt atcgaccgtg
    12721 atacatcagg cattttgctg gtggcaaaaa agcgttcggc gttacgttcc ttgcacgaac
    12781 aactacgtga gaaagtggtg caaaaagact atcttgccct tgtgcgaggg caatggcagg
    12841 ctcacactaa agtgattaaa gccccgttgc tgaaaaacga acttgccagc ggtgaacgaa
    12901 ttgtacgagt gagtgaggag ggcaaaccgt ccgaaacccg tttcagcatt gaagagcgtt
    12961 atgccaatgc cactttggtg aaagcctcgc ctattaccgg cagaacccac caaatccgtg
    13021 tgcataccca atatgccgga cacccgattg cgttagatga caaatatggt gatgtggaat
    13081 ttgacagcaa aatgaaagaa gttggcttac agcgtttatt tctgcacgcc catgctattc
    13141 gttttgaaca ccccaaaacc ggcgaagaaa tggtcattac cgcacctttg gataaaacct
    13201 taaaaggggt gttggcaaaa ttaagggcta attactaaat aacgtaatga gagagtaaga
    13261 tagcgcaagc gtccacgctt gtgctgtaaa tcaacataga gaggtacaag cgtggacgct
    13321 tgcaccaaca aaatataagc aaaaatatgc aaatccattt caaaaatttt caaaaaaatg
    13381 accgcttgtt agatttaacc acaccgcaaa ttatggggat cttaaacttc acgccggatt
    13441 cattttccga ttcgggcaag tttttttccc tcgataaagc cttgttccaa gcggaaaaaa
    13501 tgttagctga tggggcaagc attattgata ttggcggtga atccacccgc cctaatgcgg
    13561 cgattgtgag cttagaacaa gaattagagc gtgttgtgcc ggtggtggag gcgatccgca
    13621 gccgttttga ttgcttgatt tcggttgata gctccaaagc agaagtattc aaagagtcgg
    13681 caaaagtcgg tatggatatc attaacgata ttcgagcctt aaccgagccg aatgcgttgg
    13741 cagcggcagt agaattgggc ttgccggttt gtttaatgca tatgcaaggc aacccgcaaa
    13801 caatgcaaca aaatccgcac tatgatgatg tgttggaaga tgtggcagat ttcttaaatc
    13861 aacggatttt cgagtgcatc accgcaggca ttcccaaaga acacattatt ttagaccccg
    13921 gttttggctt tggtaaaagc gtgaaacaca attaccaatt attgaaacac cttaaagcct
    13981 ttgtggattc aggcttccct gtgcttgccg gactttcccg taaatcaatg attggtgcgg
    14041 tgttagataa accggtagaa caacggctac ttggctctgt agtaggggcg ttattagccg
    14101 ctcaaaatgg ggcgaaaatt ttacgggtgc acgatgtggc agaaaccgcc gatgcgttaa
    14161 aaatctggca ggcaatgctg gaatcagata atgaggaaaa atgatgacaa ccttagttta
    14221 tggcattaaa aattgcgaca cagtaaaaaa agccttgaaa tggctcgaag atcataatca
    14281 aaacccacaa ctgcacgatt accgtgtgga cggtttaaat gccgattggt tagtggaaat
    14341 ggaagcaaaa ttcggttggg aaaacttagt taataaacgt agcaccactt ggcgaaacct
    14401 tgatgaggaa attaaaaaca accttaaccg tgagtcggca ttaaaagtgc taaccgaaca
    14461 gccgacccta attaaacgcc cgattgtgat taaagaggat attgctttga ttggttttaa
    14521 tgagaaagag tatgtggctg cgtttaatta attttttccc ctgcttgtag ggacaggtcc
    14581 tgtgcctgtc cgtagtcttt aggattgttc gggcgggcac aggacccgcc cctacaggat
    14641 atatagcaaa aaattcaccc aaaacaaccg cttgtaacct atgaaaaatg aaattatctc
    14701 ccttgcacag aacctaatcc gccgtgattc catcagccca caagacaaag gctgtcagca
    14761 agaaattgca caacgtcttg aaaaacgggg ctttaatatt gaatggttgc cgtttggcga
    14821 caccttgaat ttatgggcaa cgcacggctc ggaaaatggt aaaacagagc cttgcattgt
    14881 gtttgccggg catacagatg tggtgccggt aggcgatgaa agccaatggg cttatccgcc
    14941 gttttcggct gagattgtag atggaatgct ttatgggcga ggggcggcag atatgaaagg
    15001 ctcgctctcg gcgttagttg tggcggcaga aactttcgtg caacatcacc caaaccataa
    15061 gggcaaagtg gcattattga ttacctccga tgaagaggca gcagccaaag acggtacggt
    15121 aaaagtggtt gaaaccttaa tggctcgcaa tgaagcggta cattatgcgg tagtgggcga
    15181 accgtctagt agcaaggtgt ttggcgatgt gattaaaaac ggcagacgtg gttcaattac
    15241 ggcagatctc tatatcgagg gcattcaagg gcacgttgcc tatccacatt tggcggaaaa
    15301 tccggtgcat acgtcactgg gttttttaac cgatctgacc acttaccagt gggataacgg
    15361 taacgaattt ttcccgccta ccagtttgca aattgccaat atcaaagccg gcacaggtag
    15421 caataatgtg attccgggcg agctttatat ccagtttaac ttgcgttatt gcactgaagt
    15481 aaccgatgaa atcattaaag caaaagtgac ggaaatgctc gaaaaacagg ggctaaaata
    15541 ccgaataagt tggaatttgt cgggcaaacc gttccttgcc ggcaatggga agttggtaga
    15601 agctgcatta caagcggtcg aaaatgtggc aaaaattacg cctcggttag acaccggcgg
    15661 cggtacttca gacgggcgtt ttattgcgtt aatgggggca gaagtggtgg aatttggacc
    15721 acttaatacc actattcata aagtaaacga atgtgtcagt gtggatgatt tagcgaattg
    15781 cggtgaaatt tattatcaaa ttttagaaaa attattggca aacgcataac caaaaatagg
    15841 cagaatgtta tcaaattgta ttctgcctat tttttaggta gctattctgt taaacagagt
    15901 ttacactagc agcagttttt atatttatag gaaaatgtta tgcaactcta ctcgtatatc
    15961 tgcctgctgt ttgggcttgc gatgtttatt tcgtttttta cccgaaaatt aaacgaaaat
    16021 attcaaacca ccattgctat tacagcctcc gcccttgtcg gctcccttct tattcttacc
    16081 ttcggttctc ttggctggtt tcatgttgat aaacttgcca tttccatttt tgaacagctc
    16141 gattttaaaa gcttcctact aaacggaatg cttggtttct tactgtttgc tggttcactt
    16201 ggaattaaac taccgttaat gaaagaacaa cgccgagaaa ttgcggttta tgctttactt
    16261 tctaccttaa tttccacttt cttaatcggt gctttgattt atggcgtggc aaaattatgt
    16321 ggtttggaga tcggttttgt ttattgcttg ttgttcggct cactgatttc gccaaccgac
    16381 ccgattgccg tattggcaat tatcaaaagc ttgaaagctc caaaacgcct ttcaatgcat
    16441 gttgaagggg aatcgttgtt taatgatggt atcggcttag tgatttttac cactatcttt
    16501 gctgtggctt ttggcggaca agctccaaca cttagtggca ttaccggact gttcttgcaa
    16561 gaagcggtag gcggtattgt ttttggctta ttaaccggtt ttgtggcaca taaatttatt
    16621 tctgcaaccg atgacggaag ccttgagatc ttaatcacct taaccattcc aaccgttggc
    16681 tttgtgattg cgaactactt acacgtttct ggtgcattaa caatggtcgt tgccggtatt
    16741 atgattggca actggactcg ccgttccggt ttctccgaac agagccaaaa atatcttgat
    16801 cacttctggg agatgatcga ccattcatta aactatctgt tattcctgct tatcggttta
    16861 agcacactgt tagttgattt cacctttatt ggtggggtgt taatgttggc agcaattcca
    16921 atttgtttgc ttgcacgcta catcagcctg tggattccat accaagtgtt aaaacgcttc
    16981 cgcacttata atccttacac cttacgcatt atgacttggg gcggattacg cggtggatta
    17041 gcccttgcga tggctctttc cgtaccggca ggggctgcga tggtgtcaag ttcggaaggc
    17101 gagcttgatg tgcgagattt gattctatta atgacttatg cggtggtcac tttctcaatt
    17161 ttagtacaag gttcaacggt tgaaccgatg ataaagaaat ctaagttagt tgacccaaga
    17221 agagtggcgt tgaataaatt aggcgggcct gatgatctta ttccgcatat ttaatggcat
    17281 tgatataact gaataacata tcatttcgct aaagattatt agccatctag acggctttat
    17341 atttaaatac ctctcacatt tatttgttga gaggtatttt ttatgttaat aacaggattg
    17401 ttatgcggtt ttctgcttgg atttgtgatg caaagagggc gattttgcat caccggtgca
    17461 tttcgtgatc tctatgtcac caaaaacagc cgtatgtttg tcgcattttt aattgcgatt
    17521 acagtgcaat ctatcggcat ttggctgtta tatgaagccg gctcttttag ctcaccggca
    17581 gaagatttac cattgcttgc ggtaattatc ggggcatttt tatttggtat tggcattgtt
    17641 tacgcaggcg gttgtgctac cggtacttgg tatcgagccg gtgaaggttt aattggcagt
    17701 tgggttgctc tgattgttta tgggctgttt tctgcttcta tgcgaaccgg tgtgttagct
    17761 ccacttaatc aagagctaaa aagcaatgta attgaacacc gcacgattta tgaaacattt
    17821 ggtatttccc cttgggtgtt agtgtttatt ctgagtgtaa ttacattcgc attaaccttc
    17881 tatcatttaa gaaaaccgag aggtaaaacc atcacgttaa aaccacgcaa aaccggttta
    17941 gctcatattc tgtttgaaaa acgttggcac ccgttttgac ggcagtgttg gtaggcttaa
    18001 ttgcactgct tgcttggcct cttagcagtg ctgccggtag aaacttcggc ttaggcatta
    18061 ctacgccaag tgcaaatttg gttcaatatt ttgtaaccgg tgaagttaaa tttattaact
    18121 gggcggtatt tttggtgtta gggattttga tcggctcgtt tgtggctgca aagttctcga
    18181 atgaattccg tttcagagtg ccagatgcga ccacaatgtt acgcagtgca ggaggcggtg
    18241 cattaatggg aatcggtgca agccttgccg gtggttgttc aatcgggaac ggattagttg
    18301 aaacagcttt cttctcttgg caaggctggc tttcattacc aataatgatt ctcggtacat
    18361 ttgttggagc gtattttacg attatccgcc cacagcgttt aaaaggttaa tttttttaag
    18421 gagtaataaa gatgaacgta cgtttacctg cctcagggct tgtctgcccg tttccattag
    18481 tcgaagccaa agcggcgatg gcaaaattga ataaaggcga tagcttaacg attgaatttg
    18541 actgcactca agcaaccgaa gccattccaa attgggcgga agaggaaggc tatgaagtta
    18601 ccgattacca acaacttggc gatgccttgt gggaaattac ggtggttaaa taattagcag
    18661 taaaaaagct ctactttcgt agagcttttg ctttgagttt tgtattcact aaggattaga
    18721 gtaaacccgg ttgacctaat gctgggtctg ttgctcttgc agcaatagca tcttcaaccg
    18781 tcatacctaa tgcttttgcc acaccttcac cgtatgccgg atcacaggca tagcagttac
    18841 gaatatgacg gtatttaatg aagtctaatg catcgcccat acctgcagca gtattattga
    18901 ataatgcttg tttttgttca tcattcatca tattaaacaa tacacgtggt tggctgaagt
    18961 agtcagcatc atcttcacga tagttccagt gggctgcatc accattaatt ttcagtggtg
    19021 gctctgcata ttgagcttgt tcttgccatt tgccgaagct gtttggttcg tagtgaatgg
    19081 tactaccgta gttaccgtcc acacgacctt gaccgtcacg agcgttgcta tgaaccgggc
    19141 aacgtggagc gtttacaggg atttggtggt ggttcacacc taaacggtag cgttgagcat
    19201 ccgcatagtt gaataaacga gcttgtaaca tacggtccgg agacacgctg atacccggca
    19261 ctaagttgct tggtgcgaac gcagattgtt caacatcagc aaagaagttt tcagggttac
    19321 gatttaattc aaactcaccc acttcaatta atgggtagtc tgattttggc catactttgg
    19381 ttaagtcaaa cgggtggtat ggtactttat ctgcatcagt ttcaggcata atttgaacaa
    19441 acattttcca tttcgggaaa tcgccacgct caatcgcttc gtataaatca cgttggtgac
    19501 tttcacgatc gttagcgata atagcggctg cttcttcgtt agttaagttt ttaatgcctt
    19561 gttgtgtatg gaaatggaat ttcacccaga aacgctcatt agctgcattg atgaagctat
    19621 aagtatgtga gccataaccg tgcatatgac gatatgaagc cggaatacca cgttcactca
    19681 ttacaatagt cacttggtga aatgcttctg gtaataatgt ccagaaatcc cagttgttag
    19741 ttgcagaacg catattcgtg cgtgggtcac gtttaaccgc tttgtttaaa tccgggaatt
    19801 tacgtgggtc acgtaagaag aacacagggg tgttgttacc caccatatcc cagttacctt
    19861 cttcggtata gaattttaat gcgaaaccac gaatatcacg ctcagcatct gccgcaccac
    19921 gttcacctgc aacggtggtg aaacgagcaa acatttcagt ttttttaccg acttggctaa
    19981 aaattgccgc acgagtgtat ttagtgatgt cgttagttac agtgaaagta ccgaacgcac
    20041 cggaaccttt agcgtgcata cgacgctcag gaatgacttc acgcacgaag ttcgctaatt
    20101 tttcatttaa ccataaatct tgcgctaata atggaccacg tggacccgct gttaagctgt
    20161 tttggttatc aacaacaggt gcaccgttgt tcattgtcaa atgtgtaaca ggacatttag
    20221 acataataga atcctcataa attagaaata agataggtaa ttaatccgaa gagaattata
    20281 ggtgttgtag attttttctc aatctatcaa tttgataatt aatgactatt aaatattaat
    20341 tttaaatagt tgactttaat tgtcaagtat tctgagaaag atgataagcg gtcatttttc
    20401 cactattttt tgcagtatgt aggtgtaaaa actaacggcc gagaagtaga tttcaacaaa
    20461 gaatagataa taaaaaagta catatttaag aaatatgtac tttttataat tttatttgcc
    20521 taagttatca aagaatttct tcacaccatc aaagaaacct tcgtgttt
//
LOCUS       DANKBV010000034        20008 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.034, whole genome shotgun sequence.
ACCESSION   DANKBV010000034 DANKBV010000000
VERSION     DANKBV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20008
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.034"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..510)
                     /locus_tag="PWY43_000982"
     CDS             complement(<1..510)
                     /locus_tag="PWY43_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2135873.1"
                     /translation="MFKQLQQVGKAFMLPIAILPAAGLLLGIGGALSNKATVQAYPIL
                     DNPTLQGIFQIMASAGSVVFGNLALLLCIGLGIGLAKRDKGVAALAAVVGYLIMTGTI
                     SALIPLFSPETKGIDTGVIGSLVMGLITVKLHNKYHNIQLPQILGFFGGSRFVPIVTA
                     FAAIFVGAVF"
     gene            711..2558
                     /gene="ilvD"
                     /locus_tag="PWY43_000983"
     CDS             711..2558
                     /gene="ilvD"
                     /locus_tag="PWY43_000983"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDL2135874.1"
                     /translation="MPILRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFT
                     QFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
                     RLDLVDAMIESADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSMLATHADRKELFLRAGREIVELCRKYYQQDDESVLPRSIATFDAFENAMSLDIAMG
                     GSSNTVLHLLATAQEAGVDFKMEDIDRLSRVVPCLSKIAPNTNKYHMEDVHRAGGIMG
                     LLGELDRAGLIHRHVKTVLGLTIEEQLNQYDIIRNKDEDLHKFFRAGPAGIRTTKAFS
                     QDCRWDTVDDDRVNGCIRNVENAVTKEGGLAVLFGNIAEDGCIVKTAGVDESIWKFTG
                     TAIVFESQEDAVAGILGGKVKEGHIVIIRYEGPKGGPGMQEMLYPTSYLKSMGLGKKC
                     ALLTDGRFSGGTSGLSIGHASPEAASGGAIALINDGDTVAIDIPNRSINLMVSDEELA
                     ARRAEMEARGDKAWQPLKREREVSFALKVFGHFATSADKGAVRDKSLLK"
     gene            complement(2692..3042)
                     /locus_tag="PWY43_000984"
     CDS             complement(2692..3042)
                     /locus_tag="PWY43_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HDL2135875.1"
                     /translation="MWHFYLMTIELNNIQYPTDASGYLTNLNDWSVELAEKIAEKEQI
                     SLTAEHWEIIYLVRDFYQEYKTSPAIRMLVKTMAQKFGEEKGNSRYLQRLFPDGPAKQ
                     ATKIAGLPKPAKCL"
     gene            complement(3071..3736)
                     /locus_tag="PWY43_000985"
     CDS             complement(3071..3736)
                     /locus_tag="PWY43_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor protein"
                     /protein_id="HDL2135876.1"
                     /translation="MENRLAHSYSTGESLISTHKVLRNTYMLLAMTLTFSAVVAFVAI
                     QMNAPAMPWWGLLVGFYGLLFLTNATSNSSAGILSVFALTGFLGYTLGPILNRYIGAG
                     LGDIVALAFGATALVFFACSAYVLTTKKDMSFISGMMVALFVVLLVGIIANIFLAIPA
                     LSLALSSLFVIFSSGAILLATSNIIHGGETNYIRATVDLYVSIYNLFVSFLQIFGVLG
                     SDD"
     gene            3942..4490
                     /gene="pgsA"
                     /locus_tag="PWY43_000986"
     CDS             3942..4490
                     /gene="pgsA"
                     /locus_tag="PWY43_000986"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL2135877.1"
                     /translation="MKLNFPTYLTLFRVGLTPLFIVAFYLPIKYAPEISTLIFFIASI
                     TDAFDGYLARKWNQTTRLGAFLDPVADKVLVAVAFVCVAEYYHTWWITIPICIMIARE
                     IIISALREWMAELGERASVAVSIWGKVKTTAQMLALGGMLWRQNQLMEILAFILLYIA
                     VGLTIWSMMQYLNASKNSLLKS"
     gene            4568..6406
                     /gene="uvrC"
                     /locus_tag="PWY43_000987"
     CDS             4568..6406
                     /gene="uvrC"
                     /locus_tag="PWY43_000987"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL2135878.1"
                     /translation="MSEFNAKSFLLNVPHQPGVYRMYDSKGTIIYVGKAKDLKKRLAS
                     YFRSQLSSKKTEALVANLANIETTITHSETEALLLEHNYIKENQPKYNVLLRDDKSYP
                     YILLTKHKHPRLTAFRGSKKIAGEYFGPYPNVTAVRETLNLLQKIFPIRQCEDTYYKN
                     RSRPCLQYQIGRCLAPCVEGCYSPVEYDQQVNLVRLFLQGKDHQVVEHLVQKMEDAAE
                     ALDFEGAARFRDQIQSVRAVQEKQFVSNDRLDDLDIISIVYQNGIACVHILFVRQGKV
                     LGNRSYFPKVPSNTDLTELADTFVGQFYLQMNQHRTIPNQIVIDHQLSESKALSEILS
                     EQAGHKVSILDNAIRGDKQRYLALARTNAMAALALQLKQESHIQSRYQALKEMLKMSE
                     IKRMECFDISHTMGNQTVASCVVFDENGPLKSDYRRFNIEGITGGDDYAAMEQALLKR
                     YNRELPPEKIPDIIFIDGGKGQLNRALDVFAKLDVEWDKSKPLLIGVAKGVERKAGLE
                     TLIISKWDKEIHLPPDSLALHLIQHIRDESHQHAISGHRKKRQKAFTESGLESIAGVG
                     AKRRQALLKYLGGMQGVKSATLEEIQSVPGISKALAEVIFDTLKNS"
     gene            complement(6411..6686)
                     /locus_tag="PWY43_000988"
     CDS             complement(6411..6686)
                     /locus_tag="PWY43_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135879.1"
                     /translation="MKKLILLGLSVALTACSTPQQQSTVPMDMQAVQDYHQRVINKQT
                     VDKNTKQHDEPLNQSDKRSESINLPIHPIIYPSIGVGYHYGWKRYRY"
     gene            complement(6811..7164)
                     /gene="rplT"
                     /locus_tag="PWY43_000989"
     CDS             complement(6811..7164)
                     /gene="rplT"
                     /locus_tag="PWY43_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL2135880.1"
                     /translation="MARVKRGVIARARHKKVLKAAKGYYGARSRVYRVAFQAVVKAAQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGLSYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFAALVEKAKSAL"
     gene            complement(7273..7470)
                     /gene="rpmI"
                     /locus_tag="PWY43_000990"
     CDS             complement(7273..7470)
                     /gene="rpmI"
                     /locus_tag="PWY43_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL2135881.1"
                     /translation="MPKIKTVRGAAKRFKKTASGGFKRKQSHLRHILTKKTTKRKRQL
                     RHKSMVAKADQVLVVACLPYA"
     gene            complement(7734..8276)
                     /gene="infC"
                     /locus_tag="PWY43_000991"
     CDS             complement(7734..8276)
                     /gene="infC"
                     /locus_tag="PWY43_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL2135882.1"
                     /translation="MKPVKRVQSNRAHRLNDEIRGVKEVRLTDENGEQVGIVSLQDAL
                     NRAEEAELDLVEISPNAEPPVCRIMNYGKFIYEKEKAAKEQKKKQKVVQVKEIKFRPG
                     TDDGDYQVKLRSIIRFLEDGDKVKITVRFRGREMAHQEIGMDVLERVKADTAEIAMVE
                     SAPGKLEGRQVVMVIAPKKK"
     gene            complement(8435..10234)
                     /gene="recQ"
                     /locus_tag="PWY43_000992"
     CDS             complement(8435..10234)
                     /gene="recQ"
                     /locus_tag="PWY43_000992"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL2135883.1"
                     /translation="MNTTAISVLNNIFGYQKFRDGQEEVINAVLENRDCLVIMTTGGG
                     KSLCYQVPALCLDGITLVISPLISLMKDQVDQLITNGIEAAYLNSTQTLEEQQLVEQK
                     ALSGQLKLLYLSPEKVMTQGFFHFISLCKISFIAVDEAHCVSQWGHDFRPEYTLLGGL
                     RNTFPNVPLMALTATADLTTRSDILHHLRLNSPHIYLGSFDRPNIRYTVQEKFKPLEQ
                     LIKFISKQQGKSGIVYCNSRKKVEEITEKLSARKISVMGYHAGMSFEQRERVQNAFQR
                     DDIQIVVATIAFGMGINKSNVRFVVHFDLPRSIESYYQETGRAGRDDLPSEAVLFYDP
                     SDYAWLQKILLEEPESEQRNIKQHKLQAIGDFAESQTCRRLVLLNYFGEHRQEQCKNC
                     DICLDPPQQYDGLLDAQKVMSVIYRTGQIFGQHHIIAVLRGMNNLKIREHQHDQLSVY
                     GIGKEQSQHYWQNVVRQLIHLGLIIQNIANHSALQLTESARPFLRGEKQLQLALPRLG
                     ISATAFVQKQASHRYDKDLFARLRFLRKQIADLENIPPYIVFNDATLQEMAEFLPTSK
                     VEMLEINGVGERKFERFGQAFMSLIKEYQNNRK"
     gene            complement(10343..10648)
                     /gene="cyaY"
                     /locus_tag="PWY43_000993"
     CDS             complement(10343..10648)
                     /gene="cyaY"
                     /locus_tag="PWY43_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDL2135884.1"
                     /translation="MNTAQFHQAVEQVWQQIEEKIYDEGLSVDTEIQGAVCTLTFDDE
                     SQVIINKQEAMLELWLASKLGGFHFKNVEGEWVTDEGRSFWQHLEEAFARHGESVSF"
     gene            complement(10659..11450)
                     /locus_tag="PWY43_000994"
     CDS             complement(10659..11450)
                     /locus_tag="PWY43_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL2135885.1"
                     /translation="MNNYVISLTSAQERRKHIEAEFGKQNIPFQFFDAITPDLIKEKA
                     KAFNIDISNTNLTKGEIACALSHIALWHLAKQQNLDYICIFEDDIYLGNNAFELLKTN
                     YIPENTHIVKLETLPFDRINRFNKTEKYILNRRLFKLNSRHVGTAGYILTNKGAEFLI
                     NILKTLNIPIDDLIFDEYLKIKEYKVLQMSPALCVQDFILNSKTNFKSSLQDDRAIRC
                     NNEDKIKNQAKLKKPNYFLTKIQKELYRPFKQLKQKVFTTYITFK"
     gene            complement(11532..12563)
                     /gene="tsaD"
                     /locus_tag="PWY43_000995"
     CDS             complement(11532..12563)
                     /gene="tsaD"
                     /locus_tag="PWY43_000995"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL2135886.1"
                     /translation="MRILGIETSCDETGVAIYDEDKGLVANQLYSQIDMHADYGGVVP
                     ELASRDHIRKTLPLIQEALKEANLQPSDIDGIAYTAGPGLVGALLVGSTIARSLAYAW
                     NVPALGVHHMEGHLLAPMLEENAPEFPFVALLISGGHTQLVKVDGVGQYELLGESIDD
                     AAGEAFDKTGKLLGLDYPAGVAMSKLAESGTPNRFKFPRPMTDRPGLDFSFSGLKTFA
                     ANTIKANLNENGELDEQTKCDIAHAFQQAVVDTILIKCKRALEQTGYKRLVMAGGVSA
                     NKQLRADLAEMMKKLKGEVFYPRPQFCTDNGAMIAYTGFLRLKNDEQTDLSISVKPRW
                     AMTELPPIN"
     gene            complement(12636..13415)
                     /locus_tag="PWY43_000996"
     CDS             complement(12636..13415)
                     /locus_tag="PWY43_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDL2135887.1"
                     /translation="MRKFKSLATLVLAGLFVAGCSNSNKELEQSNVQDLYGKGQTYLQ
                     DGDYNSAIRYLEAVGTKGGQYSGLGEQTQLSLIYAQYKVGEYYKALDAAERFARSYPN
                     AANMDYVFYLAGLSNARLSDNFIQDFFGVNRASRAVDNVRNAYGNFQTIVQRYPQSQY
                     AQDAQNWMNYLFNRLAEHELAVVKFYDERNAYVAVVNRIEEMLRFYPNTLATKQALPY
                     LKEAYKQMNIPDAEQKTELLIQEYESKELPNPEKPKYGEQF"
     gene            13556..14533
                     /gene="rluD"
                     /locus_tag="PWY43_000997"
     CDS             13556..14533
                     /gene="rluD"
                     /locus_tag="PWY43_000997"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDL2135888.1"
                     /translation="MTQQKILTAEITADLLGARLDQALAQLFPDYSRSRIKVWIESDL
                     VKVNGNIVNKAREKVFGGETVEITAEIEEEIRFEPQDIPLNIVYEDDDIIVINKPKDL
                     VVHPGAGNPDGTVLNALLHYYPPIAEVPRAGIVHRLDKDTTGLMVVAKNIPAQTHLVT
                     ALQKRRITREYEAIASGIMTQGGKVDEPMARHPTKRTAMAVHPMGKPAVTHYRIMERF
                     RNYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQLYGGRPRPPKGASDSFLSVLRGFQR
                     QALHATMLRLEHPITGEMMEWHAPLPDDFIELIQALKADYELYKDQLDY"
     gene            14613..14735
                     /locus_tag="PWY43_000998"
     CDS             14613..14735
                     /locus_tag="PWY43_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HDL2135889.1"
                     /translation="MKKLVLTILAAATFLTACNTVDGVGKDVEKAGDKIQQAAQ"
     gene            complement(14911..15195)
                     /gene="raiA"
                     /locus_tag="PWY43_000999"
     CDS             complement(14911..15195)
                     /gene="raiA"
                     /locus_tag="PWY43_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL2135890.1"
                     /translation="MSLNISSKQMDITPAIRTHIEERLAKLEKYHTQLINPHFMLIKQ
                     PNSFQVEATIGTPLGDLVAKAEHEDLYTAIFDVKKKLEGRLIKLKEKNEH"
     gene            15375..16316
                     /locus_tag="PWY43_001000"
     CDS             15375..16316
                     /locus_tag="PWY43_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HDL2135891.1"
                     /translation="MSEKKQLPPFQTQFLHPKYWGLWLGLGVFKLILCLPYPILVKIG
                     LGLGKLFTKLGFGKRRMRIARRNLELCFPHYSAEKVEELVEENMKSVGMAIIETGMAW
                     FWSDKRILKWSKIEGLEHLTQRDQERGILLVGVHFLTLELGARIIGLHHQGVGVYRPN
                     DNPLLDWIQFRGRVRSNKAMLDRKDVRGMIRALKNGEVIWYAPDHDYGRRNSVFVPFF
                     AVEETCTTAGSYMLLRSAPKSLVIPFSPIRNLDYSGYTVKVSPAVDFSQCQNEVETAI
                     LMNRVVEREIMAALTQYMWLHRRFKTRPNEGDQSLYD"
     gene            16412..17299
                     /gene="dapA"
                     /locus_tag="PWY43_001001"
     CDS             16412..17299
                     /gene="dapA"
                     /locus_tag="PWY43_001001"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL2135892.1"
                     /translation="MATPLFHGSIVALVTPMTHGEVDYQDLKKLVEYHIQAGSHAIVS
                     VGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGTGSNATSEAITMTKLLANSGVAG
                     CLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPGRTGSDLKPETIGRLAKIENI
                     VAVKEATGDLSRLPLIKQLAGEDFIFLSGDDATGFESMKLGGQGVISVTNNVAAADMA
                     KMCELALAGQFTEAEEINNRLMALHHDLFVESNPIPVKWAAYKLGLISEPNLRLPLTT
                     LSESAQPVVLKALQQAGLI"
     gene            17569..18795
                     /locus_tag="PWY43_001002"
     CDS             17569..18795
                     /locus_tag="PWY43_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDL2135893.1"
                     /translation="MAMPSQRFSLTWVLNLFGTAVGAGVLFLPINAGMGGFYPLIIMT
                     LLVGPMTYLAHRGLTRFVLSSKYKGSDITAVVREHFGEQAGKLITLLYFFAIFPILLI
                     YGVGITNTVSSFMENQLHIAPPSRAVLSFMLIAAMIGVMLLSEQVMLKITTCLVYPLV
                     LILLGLSIYLIPQWNTAALQQMPTTGDFLTTLWLTIPVLVFAFNHSPAISSFALSQQK
                     YYQDDKKAEIESAKVLRSTAFILVLFVMFFVFSCVLTLTPEELAQAKVQNISILSYLA
                     NKFDNPIISYFGPLVAFLAIGSSFFGHYLGAREGLEGLVNQMRKEPIEPSKFRKITAI
                     TFLIILWIVATINPSILGFIESLGGPIIAMILFIMPGYAVYKVPALARFKGEFGHLFV
                     LVMGCIAISAIVYGLL"
     gene            18852..>20008
                     /locus_tag="PWY43_001003"
     CDS             18852..>20008
                     /locus_tag="PWY43_001003"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDL2135894.1"
                     /translation="MISVFDMFKVGIGPSSSHTVGPMKAAKEFVDELQNRKFLNKIDR
                     LRVDVYGSLSLTGRGHNTDIAIIMGLMGYLPHNVDIERIEQTIENLKQNKQLIIAESS
                     VEPKVIAFDFYEDMPFHRDFLPRHENGVKFSALSGEELLFQNTYYSIGGGFIVDEANF
                     EQVKNSDLSVPFPYQNAADLLAHCKNNGLPLSSLVWKNELALRSRVEISDYLAMIWNT
                     MEACIERGLNTEGLLPGPLKVVRRASGLRRMLEANSKFNTDPMQIIDWVNMFALAVNE
                     ENAAGGRVVTAPTNGACGIVPAVLAYYQKFIAQLNQDTIERYLLTTSVIGSLYKMNAS
                     ISGAEVGCQGEVGVACSMAAAGLTEIMGGSAEQVCMAAEIAMEHNLGLTCDP"
BASE COUNT         5799 a         3666 c         4200 g         6343 t
ORIGIN      
        1 gaacactgcc ccgacaaaaa tcgcagcaaa ggcggtcaca atcggcacaa accgagagcc
       61 gccaaagaag ccgagaattt gcggcagctg aatgttgtgg tatttgttat gcagtttcac
      121 cgtaatcaag cccatcacca gcgagccgat cacaccggta tcaatccctt tggtttcagg
      181 agaaaacagc ggaatgaggg cagaaatcgt gcctgtcata atcaaataac cgaccactgc
      241 tgccagtgcc gctacgcctt tatctcgttt tgctaatcca atgcctaaac cgatacacag
      301 taataatgca aggttgccaa acaccacact gcctgccgat gccataatct ggaaaatgcc
      361 ctgtaaagtc gggttatcca gtatcgggta ggcttgcacc gttgccttat tcgacaaagc
      421 tccgccaatc cctaacagca aaccggctgc cggtaaaatc gcaatcggca gcataaaagc
      481 ttttccgact tgttgcagtt gtttgaacat aaaatttctc cttttttatg gttgtatttg
      541 atggtttata gcatactgaa atttagccgt atgaggtgtg atataactca cagatttgca
      601 aaatttttag caaattcgac cgcttgtaaa aaaattaccg cttgcggaaa aattgaagta
      661 gaattaagca tttttttagc aaacacaaca taaaaaattt ggagaagatt atgcctattt
      721 tacgttcagc aacttccact cagggtcgta atatggcggg ggctcgtgca ttatggcgtg
      781 caaccggtat gaaagaaaat gattttggta agccgattat tgcagtggtg aactcattca
      841 cccaatttgt gccggggcac gtccacttaa aagatatggg gcagttggtt gcggcagaaa
      901 ttgaaaaagc cggtggggtg gcaaaagaat tcaacaccat tgcggttgat gacggcattg
      961 caatgggaca cggcggaatg ctttattctt taccaagccg tgatttaatt gcagacagtg
     1021 tggaatatat ggtgaatgct cactgtgctg atgcgatggt gtgtatctct aactgcgata
     1081 aaatcacccc gggaatgtta atggcagcaa tgcggttaaa tattccgact atttttgtct
     1141 ctggtggccc gatggaagcc ggaaaaacta aactttccga tcaattaatt cgcttggatt
     1201 tagtcgatgc aatgatcgaa agtgccgacc cgaatgtgtc tgatgaacgt attgatgcga
     1261 ttgaacgtag tgcttgcccg acttgtggtt cttgttcagg tatgttcacc gcaaactcaa
     1321 tgaactgttt aactgaagct ttaggcttaa gtttaccggg caatggttca atgttggcaa
     1381 ctcatgctga ccgtaaagag cttttcctaa gagcaggtcg tgaaattgtg gaactttgcc
     1441 gtaaatatta ccaacaagac gatgagagcg tgttacctcg ctcaatcgct accttcgatg
     1501 catttgaaaa tgcaatgagc cttgatattg caatgggtgg ttcaagtaat accgtgcttc
     1561 atttactagc gactgctcaa gaagccggcg ttgattttaa aatggaagat attgaccgcc
     1621 tctctcgtgt cgtgccgtgc ttaagtaaaa ttgccccgaa taccaataaa taccatatgg
     1681 aagacgtaca ccgtgccggt gggattatgg gcttgctggg cgaattagat cgtgcaggtt
     1741 taatccatcg ccacgttaaa acagtgctag gtttaacgat agaagagcaa ctgaatcaat
     1801 acgatattat tcgtaataaa gatgaagatt tacacaaatt cttccgtgca ggtccggcag
     1861 gaattcgtac tactaaggct ttctcgcaag attgccgttg ggacactgtt gatgatgacc
     1921 gtgtaaacgg ctgtattcgc aatgtggaaa atgctgtgac caaagagggc gggttagcgg
     1981 tattatttgg gaatattgca gaagatggat gtattgttaa aaccgcaggg gtagatgagt
     2041 ctatttggaa attcactggc accgcaatcg tgtttgaaag ccaagaagat gcggtagcag
     2101 gtattttagg cggaaaagtg aaagaagggc atattgtgat tatccgctat gaaggtccga
     2161 aaggcggccc gggtatgcaa gaaatgcttt atccaactag ttatttgaaa tcaatgggct
     2221 taggcaaaaa atgtgcttta ttaactgacg gtcgtttctc tggcggtacc tcagggttat
     2281 ctatcggaca cgcttcaccg gaagccgctt caggaggagc gattgcctta attaatgatg
     2341 gcgatacggt ggcgattgat attccgaatc gttcgattaa cttaatggtt tcagatgaag
     2401 aacttgctgc tcgccgtgca gaaatggaag cccgtggcga taaagcgtgg cagccgctaa
     2461 aacgtgagcg agaagtgtcg tttgcactaa aagtgtttgg acattttgcc acctctgctg
     2521 ataaaggtgc ggtgagggat aaatcgctgt taaagtaaat aaattagaaa aaagaagcat
     2581 ttcaataata tattgatctg ctctttattc agctgaatat ttaatctagc tgaatggaga
     2641 gcagatcgat agcctaattt acatcttttt gttttgatac agggaaatgt attacaagca
     2701 ttttgccggt tttggcagac ctgcaatttt agtggcttgt tttgcaggac catcaggaaa
     2761 caagcgttgt aaataacggc tgttgccttt ttcttcaccg aatttttgtg ccattgtctt
     2821 taccagcatt cgaattgctg gtgaagtttt gtattcttgg tagaaatccc gcaccagata
     2881 aataatttcc caatgttctg cggttaagct aatttgctct ttttcggcga ttttttctgc
     2941 taattctaca gaccaatcat ttaaattagt taaatagcct gaagcatcgg ttgggtattg
     3001 gatattattt aattcaatcg tcatcaaata aaaatgccac ataatgtggc attcctctta
     3061 aactaaaacg ctagtcgtca ctacctagca caccgaagat ttgtaagaag cttacaaata
     3121 ggttgtagat agacacatat aaatcaacgg ttgcacgaat gtagttggtt tcgccaccgt
     3181 gaataatgtt gctggttgcc agtaaaatcg caccgcttga gaaaatgaca aacaagctgc
     3241 ttagggctaa acttaatgcc ggaattgcta agaagatatt ggcaataata ccgactaata
     3301 ataccacaaa cagggcaacc atcattccgc tgataaagct catatctttt ttggtggtta
     3361 atacataagc tgaacaagcg aagaaaacca atgcggttgc accgaatgca agggcgacaa
     3421 tatcccctaa acccgcaccg atgtaacggt ttaaaatagg acctaaggta tagcctaaga
     3481 agccggttaa agcaaatacg cttaagatac ccgcactgct gtttgaggtg gcattcgtca
     3541 agaaaagtaa gccgtaaaag ccgaccaata atccccacca cggcatagcc ggtgcgttca
     3601 tttgaattgc aacaaaagca acaactgcgg agaaagtcag ggtcattgcc aataacatat
     3661 aggtattgcg aagcactttg tgggtgctaa ttagagattc accagttgaa tagctgtgag
     3721 ctaaacgatt ttccataaaa attccttctg tttttgtgtt taaatcggaa catccgatgg
     3781 attaatatct atggataaat ttccataaat caagaagtaa ggtagccgta atttattgta
     3841 aagtcaatgc gattttgtaa cagaagagta aaaaaaaaca tttagttgtg ttacacttaa
     3901 taccactcta ctttatattc cttttcatcg atgaattcgc tatgaagctc aatttcccaa
     3961 cttatttaac cttatttcgg gtaggattaa ccccactttt tattgtggct ttttatctgc
     4021 cgataaaata tgcgcctgaa atttcaacac ttattttttt tattgcatcg attaccgatg
     4081 cctttgacgg ctatttagca cgcaaatgga atcaaaccac ccgacttggt gcatttttag
     4141 atcctgttgc ggataaagtc ttggtagctg ttgcgtttgt ctgcgtggcg gagtattacc
     4201 atacttggtg gattactatt ccaatctgca ttatgattgc ccgtgaaatt attatttcgg
     4261 ctttgcgtga atggatggcg gagcttgggg aaagggccag tgttgccgtt tcgatttggg
     4321 ggaaagtaaa aacgactgct caaatgctcg cattaggtgg aatgttatgg agacagaacc
     4381 aactaatgga gattttagcc tttattctgc tttatattgc agtgggttta acgatctggt
     4441 ctatgatgca gtatttaaat gcttcaaaga atagtctatt gaagtcataa gcggtcatat
     4501 ttttattctt ttttgcaatt tgtagagtgg aaatatcgct ctacaatttt caattttatc
     4561 aactctaatg tctgaattta acgctaaatc cttcctgtta aatgtgcctc accaaccggg
     4621 tgtttatcgt atgtatgata gtaaaggcac gataatctat gtgggtaaag ccaaagattt
     4681 aaaaaagcgt ttagccagct attttcgtag ccaactttcc agtaaaaaaa cggaagcctt
     4741 ggtggcaaat cttgctaata ttgaaacgac gatcactcat tctgaaacag aggcgttact
     4801 ccttgaacat aattatatta aggaaaatca gccgaaatat aatgtgttgc tcagagatga
     4861 caaatcttat ccttatattc tgttaactaa acataagcat cctcgtttaa cagcatttcg
     4921 tggtagtaaa aagatcgccg gtgaatattt tgggccttat ccgaatgtta cggcagttcg
     4981 ggaaacactg aatttattgc aaaaaatttt tcctattcgt cagtgtgaag atacttatta
     5041 taaaaaccgt tcacgacctt gcttacagta tcagatcgga cgctgtttag ccccttgtgt
     5101 agagggctgt tattcacccg ttgaatacga tcagcaggtt aatttggttc gtttgttttt
     5161 acagggaaaa gatcaccaag tagtcgagca tctggtacag aaaatggaag atgcggcgga
     5221 ggctttggat tttgaaggtg cggcacgttt tcgggatcaa attcaatcag taagagcggt
     5281 gcaagaaaaa caattcgtct ctaatgatag gctagatgat cttgatatta tctcgatcgt
     5341 ttatcaaaat gggattgcct gtgtgcatat cttatttgtt aggcaaggta aggtactagg
     5401 caatcgttct tactttccaa aagtgccaag taatacagat ttaaccgagc tcgcagatac
     5461 ctttgtcggt cagttttatt tacaaatgaa ccagcaccgc accataccta accaaattgt
     5521 aattgatcat caattaagtg aatctaaggc actcagtgaa attttaagtg agcaagcagg
     5581 gcataaagtc agcattttag ataatgccat cagaggtgat aaacagcgtt atttagcttt
     5641 agcgagaact aatgcaatgg cagcattagc tttacaactt aagcaagaat cccatattca
     5701 aagccgttat caggcattga aagaaatgtt gaaaatgtca gagattaaaa gaatggagtg
     5761 ctttgacatc agtcatacaa tgggcaacca aacagtggct tcttgcgtgg tgtttgatga
     5821 aaatgggccg ctaaaatcgg attaccgccg ttttaacatt gaggggatta ccggaggtga
     5881 tgattatgca gcaatggagc aggctctctt gaaacgctat aatcgagaat taccgccaga
     5941 aaagatccct gatattattt ttattgatgg tggcaaaggg cagttgaacc gagcgttaga
     6001 tgtcttcgct aaacttgatg tggaatggga taaatcgaaa ccgcttttaa ttggtgtggc
     6061 aaaaggcgta gaacgtaaag ctgggctgga aactttaatt atcagcaaat gggataaaga
     6121 aattcactta ccgccagata gtcttgcctt gcatttaatt cagcatattc gagatgaatc
     6181 ccaccagcat gccattagtg ggcatcgtaa aaaacgccaa aaggccttta ccgaaagtgg
     6241 attagaatct attgccggtg tcggtgcaaa acgccgacaa gcgttgctaa aatatttagg
     6301 cggaatgcag ggggtgaagt ctgcaacctt ggaagagatt caatcagttc cgggaatttc
     6361 taaagcgttg gcagaggtta tttttgatac attaaaaaat agttaaatta tcaatagcga
     6421 tatcgcttcc agccgtaatg gtagcccact cctatgcttg ggtaaattat agggtgaatt
     6481 ggcagattta tgctttctga gcgtttatcg ctttggttta atggctcatc atgttgcttt
     6541 gtatttttat caacagtttg cttattgatt actcgttgat ggtaatcctg aactgcttgc
     6601 atatccattg gtactgtgct ttgttgttgg ggtgtcgaac aggcggttag tgcaacgctc
     6661 aatccgagta aaattaattt tttcatatca tttccttata ttgcaaaata ttttgctttt
     6721 ttgaccgctt attttgctat taattcttaa aaagattatt ttatccataa aaaaggacgc
     6781 actaggcgtc ctcatttcta ttctttaaaa ttaaagagca gattttgctt tttcaactaa
     6841 agctgcgaaa gcaactttgt caaatactgc gatgtcagct aagatcttac ggtcgatttc
     6901 aacagatgct tttttcaagc cgttgatgaa tttgctgtaa gataaaccgt tttgacgagc
     6961 tgctgcgtta atacgagcaa tccataattg acggaattga cgtttacgtt gacggcggtc
     7021 acggtaagca tattgagcag ctttaactac tgcttggaaa gcaacgcgat acacgcgtga
     7081 acgtgcacca taataacctt tagcagcctt aagaactttc ttatggcgtg ctcttgcaat
     7141 aacaccacgt tttacacgag ccattattta atctcctaat tgtctatttt gattggtggt
     7201 cgtcatcacg acctttacta agaattctat ccgtacgtcc gtactttact acactacgct
     7261 aacctaaaag gcttatgcgt atggtaagca cgctactact aaaacttggt cagctttcgc
     7321 taccattgat ttgtgacgta attgacgttt acgtttagtg gttttcttag tcaaaatgtg
     7381 acgtaagtga gattgtttac gtttaaaacc gccggaagct gtttttttga aacgcttagc
     7441 agcaccacgt actgttttaa ttttaggcat tgttaaaaaa ctccgcattg tttgttagat
     7501 attatgaaaa taaggcgaat tttgtgaaca aaattacttg gaagcactta ttctccgcta
     7561 tacgattttc atcgttaaaa agtcaagcag gctgctgagt ttgcctgttg agcattttct
     7621 ctttcttggc taggcaaaaa agcttgcgaa ttttagcgaa aatataaccg ctatgcaagc
     7681 caatttaagc tttatcaaga aaaaagcaca ttaaaacaat gtgcttatcg aaattatttc
     7741 ttcttaggtg cgatcaccat cacaacttgg cgaccttcca atttgcctgg ggctgactct
     7801 accattgcaa tttctgcagt atctgctttg acacgttcta aaacgtccat accgatttct
     7861 tggtgagcca tttcacgacc acggaaacgc actgtgattt ttactttatc cccatcttct
     7921 aagaaacgga taatgctacg aagtttaact tgataatcgc catcatcagt tcccggacgg
     7981 aatttgatct ctttaacttg aacaactttt tgttttttct tttgttcttt tgctgctttc
     8041 tctttttcgt agatgaactt accatagttc ataatacgac aaactggtgg ttcagcattt
     8101 gggctgattt ccactaaatc caattcagct tcttctgctc tatttagagc atcttgaagt
     8161 gatacaatac caacttgttc gccgttttca tcggttaagc gaacttcttt tacgccacga
     8221 atttcgtcat taagacgatg tgcacgattt gattgaacac gttttacagg tttaatagcc
     8281 ttattccttc tagtaaattt ttgcttaaat tttaccgctt gagagcgtat aaatatttta
     8341 gattgcttgt tttaagtgat taaaaaacag ctaaacgtgc ggattctata agattttcag
     8401 tgcttatgca ataataaagc ccttatttaa ctgcctattt tcggttattt tgatattctt
     8461 tgattaaact cataaatgct tgcccaaatc gttcaaattt tcgttcacca acaccattaa
     8521 tttctaacat ttccactttt gaagtcggga gaaattctgc catttcttgc aaggtggcat
     8581 cgttaaagac gatataaggt ggaatatttt cgaggtcagc tatctgcttg cgtaaaaaac
     8641 gtaagcgtgc gaataggtct ttgtcatagc gatgacttgc ttgtttttgc acaaatgcag
     8701 tcgcagatat acctaaacgt ggcaatgcaa gctgaagttg cttttctcca cgcaaaaacg
     8761 gtctggcact ttcggttaat tgcagagcgg aatggttagc aatattttga ataatcaacc
     8821 ctaaatgaat cagttgccgc accacatttt gccaataatg ttggctttgt tcttttccga
     8881 taccataaac ggaaagttga tcgtgttgat gttcacgaat tttgagattg ttcattcctc
     8941 gcaataccgc aataatatgg tgctgaccga aaatttgacc ggtgcggtaa atcaccgaca
     9001 tcactttttg ggcatcaagt aagccatcgt attgttgcgg tggatctaaa caaatatcgc
     9061 agtttttgca ttgctcttgg cgatgttccc caaagtagtt gagtaagact aaacggcggc
     9121 aagtctgtga ttcggcgaaa tcgccaattg cctgtaattt atgctgttta atatttctct
     9181 gttcgctttc cggctcttcc aataatattt tttgtaacca agcataatcg ctaggatcat
     9241 aaaaaagcac ggcttcagaa gggagatcat ctcgcccggc tcgtccggtt tcttgatagt
     9301 aagattcgat agagcgaggt aaatcgaaat gtaccacaaa acgtacgttg gatttgttga
     9361 tccccatacc aaacgcaata gtggcaacca caatttgaat atcatcacgc tgaaaggcat
     9421 tctgcactcg ttctctctgt tcaaaactca taccggcgtg atagcccatc acgctgattt
     9481 tacgggctga gagtttctcg gtgatttctt ccactttttt gcgactgttg cagtaaacaa
     9541 taccgctttt gccttgctgt ttgctgatga atttaatgag ctgttcaagc ggtttaaatt
     9601 tttcctgcac ggtgtagcga atattggggc gatcaaagct gcctaaatag atatgtgggc
     9661 tatttaagcg taaatgatgc agaatatcgg agcgggttgt tagatctgcc gtggcggtga
     9721 gtgccatcaa cggcacattc gggaaagtgt ttcgtaagcc acctagcagg gtatattcag
     9781 ggcggaaatc gtgcccccat tgggaaacac aatgggcttc atcaaccgca ataaagctga
     9841 ttttacatag cgaaataaag tggaaaaagc cttgagtcat taccttttca ggggaaagat
     9901 agagtaattt taattgaccg gataaggctt tttgttccac aagttgctgc tcttcgagcg
     9961 tttgagtgga gttaagataa gctgcttcaa tgccgttggt gataagttga tccacctggt
    10021 ctttcatcaa cgaaataagc ggcgaaatca ccaaagtgat gccgtctagg cacaaagccg
    10081 gcacttggta gcaaagggat ttgccaccgc ctgttgtcat aatcacgagg caatcacgat
    10141 tttccaacac ggcattgata acttcttctt gcccgtctcg gaatttttga tagccaaaaa
    10201 tattatttaa aacagaaatt gcggtggtgt tcattggtag ttggttgcta cttatgatag
    10261 tactcgtctt ctgagagtat cttttaaaat gaaaataaag cactcatcaa agacgagtgc
    10321 tgtcttagtt aacaattagg cattagaaag aaacgctttc tccgtgtcga gcaaaagctt
    10381 cttctaaatg ttgccagaac gagcgacctt catctgttac ccattcgcct tctacatttt
    10441 taaagtggaa accacccaat ttgctggcaa gccataattc cagcattgcc tcttgtttat
    10501 taatgataac ttgcgattca tcatcaaagg ttaaggtgca gaccgctcct tggatttcag
    10561 tatcgactga taatccttcg tcataaattt tttcttcaat ttgttgccat acttgctcta
    10621 cggcttggtg gaattgtgct gtgttcattg gtgtatcctt atttgaatgt tatataagta
    10681 gtaaatactt tttgttttag ctgtttaaat ggtctgtaaa gttctttttg tatttttgtt
    10741 aagaaataat taggcttttt aagtttagct tgatttttta ttttatcctc attattacaa
    10801 cgaatagctc tatcatcctg taatgagctt ttaaaattag ttttactatt taaaataaaa
    10861 tcttgtacac ataaagcagg agacatttgt aatactttat attctttaat ttttaaatat
    10921 tcatcaaaaa ttaagtcgtc aataggaatg ttaagtgttt ttaatatatt aattaaaaat
    10981 tctgctccct tgttcgttaa tatatagcca gccgtaccaa catgtctact gtttaattta
    11041 aaaagccttc tatttaatat gtatttctcg gttttgttaa aacgatttat tctatcaaat
    11101 ggtagtgtct ctaatttaac aatatgagta ttttctggta tataatttgt ttttagtaat
    11161 tcaaatgcat tattacctaa ataaatatca tcttcaaaaa tacaaatata atccaaattc
    11221 tgttgtttgg ctaaatgcca taatgcaata tgactcaaag cacaggctat ttcacctttt
    11281 gttaagtttg tatttgaaat gtcaatatta aatgctttgg ctttttcttt aattaaatcc
    11341 ggcgtaatcg catcgaaaaa ttgaaaagga atattttgtt tgccgaattc agcttcaata
    11401 tgttttctac gctcctgtgc ggaggttagg ctaattacat aattattcat aaatttaatc
    11461 tatcatatag caagcggtta tttttgatta aatttttgca aaattaacaa gaaatttcac
    11521 cgcttgaaag gttaattaat cggtggtaat tcggtcatag cccagcgggg ttttacgcta
    11581 atgcttaagt cggtttgttc atcgtttttt aagcgaagaa agccagtgta ggcaatcatt
    11641 gcgccgttgt cagtgcaaaa ttgtgggcga gggtagaata cttcgccttt taattttttc
    11701 atcatttccg caaggtctgc tcgtaattgt ttattggcac ttacgccgcc tgccattact
    11761 aagcgtttat agccggtttg ctctaacgct cgcttgcatt taattaaaat agtatcaacc
    11821 acggcttgtt ggaatgcgtg ggcaatatcg catttggttt gctcatcgag ttcaccattt
    11881 tcattaagat tggctttaat cgtattcgca gcaaaggttt ttaaaccgga gaaactgaaa
    11941 tccagtcccg gtctgtcggt cattggacga gggaatttaa aacgatttgg cgtgccggat
    12001 tcggctaatt ttgacatcgc tacaccggca gggtaatcca aaccgagtag tttgcctgtt
    12061 ttgtcaaagg cttcaccggc agcatcatca attgattccc cgagtagttc gtattgccca
    12121 acgccgtcaa cttttaccag ttgggtgtgt ccacctgaaa tcaataatgc cacaaacgga
    12181 aattcagggg cattttcttc caacattggg gcaagtaaat gcccttccat atggtgaacg
    12241 cccaatgccg gaacattcca agcataagcc agcgaacggg caatggttga gccgaccaat
    12301 aaagccccga ccaagcctgg gccggcagta taggcaatgc cgtcaatatc cgagggttgc
    12361 agattggcct cttttaaggc ttcttgaatt agtggcaacg ttttacggat atggtctcga
    12421 gaagccagtt cagggactac gccaccgtaa tcggcgtgca tatcaatttg gctataaagc
    12481 tggtttgcca ctaagccttt gtcttcatca taaatggcaa caccggtttc atcacaagag
    12541 gtttcaatac ctaaaattcg catagttgga gaagtcaaaa taagttagaa aaccaaaagg
    12601 ggcgaagaac gcccctcaca ttattctctg tacgactaga attgttcgcc atacttcggt
    12661 ttttcaggat tcggtagctc tttgctttca tattcttgga tcaagagttc cgttttttgc
    12721 tctgcatctg ggatattcat ttgcttatat gcttctttta agtatggcaa tgcttgtttg
    12781 gttgccagtg tattcgggta aaaacgcaac atttcttcga tacggtttac tacagccaca
    12841 taggcattac gttcatcata aaatttaaca acggctaatt cgtgttccgc taaacggtta
    12901 aataagtaat tcatccaatt ttgagcatct tgtgcatatt ggctttgagg gtagcgctgt
    12961 acaatagttt ggaagttacc gtaagcatta cgaacattat ctacggctcg agaggcgcga
    13021 tttacaccaa agaagtcttg aataaaatta tcacttagac gtgcatttga taatcctgct
    13081 agatagaata cgtaatccat attagctgca ttcgggtaag agcgagcaaa acgttctgcg
    13141 gcatcaagtg ctttatagta ttcgccaact ttatattgag cataaattaa acttagttgg
    13201 gtttgctcac caagtcctga atattggcct cctttggttc caaccgcttc taagtagcga
    13261 atggcagaat tataatctcc atcttgtaaa taggtctgac ctttgccata aagatcttga
    13321 acgttggatt gttctaattc tttatttgaa tttgaacaac ctgcaacaaa cagtccggct
    13381 aatactaatg tggcaagaga tttaaattta cgcatatgtt ttacctataa aaagtgtcta
    13441 ggacgaaaag ttaatgtaca atgtcctctg actaagaata acttatgaag ttcatctcgc
    13501 tattctatat aacatcacgc aagtaagcaa atgactaatt aggatttatg gtttaatgac
    13561 tcaacaaaag attttaacag cagaaattac tgcagattta ctaggtgctc gcctagatca
    13621 ggctctggca caactttttc cggactattc tcgttcacgc attaaagtgt ggattgaaag
    13681 tgatctagtg aaagttaatg gcaatatcgt gaataaagca agggaaaaag tatttggtgg
    13741 tgaaactgtt gaaattaccg ctgaaataga agaggaaatt cgttttgagc ctcaagatat
    13801 tcctctaaat attgtgtatg aagatgacga tattatcgtc attaataaac cgaaggattt
    13861 agtggttcac ccaggggctg gtaatccgga cggtacagtg ctgaatgctt tgctacatta
    13921 ttatccaccg attgcagaag tgcctcgagc agggattgtt caccgattgg ataaagacac
    13981 gacaggctta atggtagtgg ctaaaaacat tccggcacag actcacttag taaccgcatt
    14041 acaaaaacgt cgtattaccc gtgaatatga agcgattgcc agcggcatta tgacacaggg
    14101 cggaaaagtt gatgagccaa tggctcgcca cccaactaaa cgtacggcaa tggctgttca
    14161 cccaatggga aaaccggcgg ttacgcatta tcgtattatg gagcgttttc gtaattacac
    14221 acgtttacgt ttaagattag aaacagggcg tactcaccaa attcgggtac atatggcgca
    14281 tattgctcac ccattattag gtgatcagct ctatggcggt agacctcgcc cacctaaagg
    14341 tgcgagtgac tcgtttttat cagtgttacg aggcttccaa cgccaagcct tacacgccac
    14401 aatgttgaga ttagagcatc cgattactgg tgaaatgatg gagtggcacg ccccgttacc
    14461 tgatgatttt attgaattga ttcaggcact aaaagcggat tatgagcttt ataaagatca
    14521 attagattat taagtttagg tgttgcttaa ataatgtaaa gttgatatat tgaaatcgtt
    14581 ttcataagta aacaacaaaa aaggagcttt ctatgaaaaa attagtatta actattttag
    14641 cagctgcaac tttcttgact gcgtgtaata ccgttgatgg tgttggtaag gatgttgaaa
    14701 aagcgggcga taaaattcag caggctgcac aataacaaaa agcttcgaat tctaagaagc
    14761 taataatata tataccttat tgaacaattg ttcaataagg tatatttttg cgaaaaatac
    14821 ggtaaatttt accgcttact cttggaataa aaaatagggc taaatgatat ctctcattta
    14881 gcccttatta ttagatgtaa agtttaaaca tcaatgttca tttttctctt ttagtttaat
    14941 taaccgacct tctaatttct tcttaacatc aaaaattgca gtatataaat cttcatgttc
    15001 agctttagcg actaaatcac ctaaaggggt gccaattgtt gcttcaactt ggaatgagtt
    15061 aggttgtttt atcagcataa aatgtgggtt tatcaattgg gtgtggtatt tttctaactt
    15121 agctaaacgc tcctcaatat gagtacggat tgcaggggta atgtccattt gtttgcttga
    15181 aatatttaat gacataacat tttcctcttg tttagttaag gcttttgccg ttgatattct
    15241 aataaccaaa atatgctcat cactccaaaa attcaagtga aaaatgaaat aagtttccaa
    15301 aaatttgatc tacttaacac ttttagcagt ttttagctat aatgtaatca taatctagta
    15361 tgtgagaata attaatgagt gagaaaaaac agttacctcc attccagaca caatttttac
    15421 accctaaata ttggggattg tggctcggtt taggtgtttt taagttaatt ttatgtttgc
    15481 cttatcctat tttagtaaaa attggtctgg gactaggtaa attgtttact aaattgggtt
    15541 ttggtaaacg tcgtatgcgt attgctcgcc gtaatttaga gctctgtttt ccccattact
    15601 ctgctgagaa agtagaggaa ttagtcgaag aaaatatgaa atcggttgga atggcgatta
    15661 ttgaaaccgg tatggcttgg ttttggtcgg ataaacgtat tcttaaatgg tctaaaatcg
    15721 aagggctaga gcatttaaca cagcgtgatc aggaaagggg tattttactt gtaggggtgc
    15781 attttctgac tttagaactc ggtgcaagaa ttattgggct acatcatcaa ggagtagggg
    15841 tttatcgacc gaatgataat ccattattag attggataca gtttcgagga cgtgttcgct
    15901 caaataaagc gatgttagat cgtaaagatg tgcgtggaat gattcgagct ttaaaaaatg
    15961 gcgaggtgat atggtacgcg cctgatcacg attatggaag acgaaatagc gtttttgtac
    16021 cattttttgc agtggaggaa acttgtacta ctgcgggtag ttatatgtta cttcgttctg
    16081 ccccaaagag tttagtgatc ccgtttagcc cgattcgaaa tttggactat tcaggctaca
    16141 cagtaaaagt cagtcctgcc gttgatttta gtcaatgtca aaatgaagtt gaaacggcta
    16201 ttttaatgaa tcgggtggta gaacgtgaaa ttatggctgc gctaactcaa tatatgtggc
    16261 tacatcgccg ttttaaaact cgcccaaatg aaggtgatca aagcctatat gattaatttt
    16321 aaataatttc aaaaaagtgc ttgcatattt aagctgaatt ttgcaatcta gcaatgttat
    16381 caagcataat aaaaaaatac aggagatgat tatggcgacc cctttatttc acggtagtat
    16441 cgtagcatta gttacaccaa tgactcatgg tgaggtagat tatcaggatt taaaaaaatt
    16501 agttgagtat catattcaag caggttctca cgctattgta tctgttggta caacaggtga
    16561 atctaccaca ttaagcattg atgaaaatgt taaggtgatt aagaaaacgg ttgaatttgc
    16621 tgatggacgt attccaatta ttgcaggaac tggttctaat gcgaccagtg aagctatcac
    16681 tatgactaaa ttattagcaa acagtggtgt agctggttgt ttaagtgtag tgccttatta
    16741 caacaagcca acgcaagagg ggatgtactt acactacaaa gcgattgcag aaagtacaga
    16801 tttaccacaa attctgtata atgtgccggg gcgaacaggg agtgatttaa aacctgaaac
    16861 aatcggtcgc ttagcgaaaa ttgagaatat tgttgctgtg aaagaagcaa cgggtgattt
    16921 aagccgctta cctttaatta agcaattagc cggtgaagat tttattttct taagtggtga
    16981 tgatgctacc ggttttgagt ctatgaaatt aggtggtcaa ggggtgatct cggtcaccaa
    17041 taatgttgct gctgctgata tggcaaaaat gtgtgagtta gctcttgccg gtcaatttac
    17101 tgaagcagaa gaaattaata accgcttaat ggcgttacac catgacttat ttgtcgaatc
    17161 taacccgatt ccggtgaaat gggctgctta taaattaggt ttaattagtg agcctaattt
    17221 acgtttaccg cttaccacac tgtctgaatc agcacagcct gtagtgttaa aagcattaca
    17281 acaagcgggc ttaatttagt tattttttgc aaatcaaatt tgaaaccggt taagttaatt
    17341 ctaaccggtt ttttcatatc taaaagaaat ttccacataa aaataaaatt ctaaatttct
    17401 ttgatttaag taaaatttaa ttttgtttga tggattttta ctcgccaaaa acactatttt
    17461 ttctgctttt aatagtttga tcaaaaaata cgtaaaatct tgttgtgtta ctgtttccca
    17521 tcgctaatat agcatagctt gttagataat tctttaaagg agattactat ggctatgcca
    17581 tctcaacgtt tttctcttac ttgggtacta aatttatttg gtactgcggt aggagccggc
    17641 gttttatttt tgccaattaa tgcaggtatg ggtgggttct acccacttat tattatgact
    17701 ttgttagttg gaccaatgac atatttagct caccgtggct taacacgttt tgtgctttct
    17761 tcaaaataca aagggagcga tattacggct gttgttcgtg aacattttgg tgaacaggcc
    17821 ggtaaattga tcacgctact gtacttcttt gccatttttc cgattttact gatttatggt
    17881 gtaggtatta ccaataccgt gagttctttt atggagaatc agcttcatat cgcgccacca
    17941 tctagggctg tattatcgtt tatgctaata gctgctatga tcggagtaat gttattaagt
    18001 gagcaagtca tgttaaaaat cacaacttgt cttgtgtatc cattagtgtt gattttattg
    18061 ggcttatcta tttatttaat tccacagtgg aatacagctg ccttacagca aatgccaact
    18121 acaggagact tccttactac actttggctg actattcctg ttttagtctt tgcttttaac
    18181 cattcgccgg ctatttcttc ttttgcactt tctcaacaga aatattacca agatgacaag
    18241 aaggctgaaa ttgaatcagc taaagtatta cgttctaccg cctttatctt ggtgttattt
    18301 gtgatgttct ttgtatttag ctgtgtatta actttgacac ctgaagaatt agcacaagct
    18361 aaagtgcaaa atatttcgat tctttcttac cttgcgaata aatttgataa tccgattatt
    18421 tcctattttg gaccgttggt tgcttttttg gcgattggta gctctttctt tgggcattat
    18481 ttaggtgcgc gcgaggggtt agaaggattg gttaatcaaa tgcgtaaaga gccgattgag
    18541 ccaagcaaat tccgcaaaat taccgctatt acttttttga tcattttatg gattgttgcg
    18601 actatcaatc caagcatttt aggcttcatt gaaagccttg gtgggccgat tattgcgatg
    18661 atcttgttta ttatgcctgg ctatgccgta tataaagtac cggcacttgc acgttttaaa
    18721 ggtgaatttg gccatttatt tgtgcttgtt atgggttgta ttgctatttc agcaatagta
    18781 tatgggttac tgtaatcttt ctaaaatcaa tgtagaatac gcccttttat tttctatgtg
    18841 aatttgtgtt tatgattagt gtatttgata tgtttaaagt ggggattggg ccttccagtt
    18901 cccatactgt ggggccgatg aaagcggcaa aggaatttgt tgatgagcta caaaatcgca
    18961 aatttttaaa caaaattgac cgcttgcggg tagatgtcta tggttcatta tcactcaccg
    19021 ggcgaggaca taatacggat attgccatca taatgggatt aatggggtat ttgcctcata
    19081 atgtggatat tgaacgtatt gagcagacga ttgaaaatct aaaacaaaat aagcaactta
    19141 tcattgctga aagcagtgtt gagcctaaag tgattgcgtt cgatttttat gaggatatgc
    19201 catttcatcg agatttctta cctcggcacg aaaatggtgt gaaatttagt gccttgagcg
    19261 gtgaagagtt gctatttcaa aacacctatt attctattgg tggtggcttt attgttgatg
    19321 aagctaactt tgagcaagtt aaaaattcag atctttctgt tccattccca tatcagaatg
    19381 cagcagattt acttgcacat tgtaagaata acgggttacc gctttcttct ttagtctgga
    19441 aaaatgagct tgctttacgc agtagagtag aaatttcgga ttatttggca atgatttgga
    19501 atacaatgga agcctgtatt gagcgtggtt tgaatacgga agggttacta cccgggcctc
    19561 tgaaagtggt acgccgtgcc agtgggctac gcagaatgtt agaggcaaac agtaaattca
    19621 ataccgaccc aatgcaaatt atcgattggg tgaatatgtt tgcccttgcc gtgaatgaag
    19681 aaaatgcggc aggtgggcga gtagtcaccg cacctaccaa tggagcctgt ggtattgttc
    19741 cggctgtgtt ggcgtactac caaaaattta ttgcacagct taatcaagat actattgagc
    19801 gttatttatt gactaccagt gtgattggtt cgttatacaa aatgaatgcc tctatttccg
    19861 gtgctgaagt aggttgccaa ggggaagttg gtgttgcttg ttcaatggcg gcagcaggtt
    19921 taacggaaat tatgggtggc agtgctgaac aggtgtgtat ggctgcagaa attgcaatgg
    19981 agcacaattt aggcctaact tgtgaccc
//
LOCUS       DANKBV010000035        19939 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.035, whole genome shotgun sequence.
ACCESSION   DANKBV010000035 DANKBV010000000
VERSION     DANKBV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19939
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.035"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            24..521
                     /locus_tag="PWY43_001004"
     CDS             24..521
                     /locus_tag="PWY43_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2135895.1"
                     /translation="MAKKGEVKATSKSRGQPTKYKPEYVTQVEKLCLLGATDKDIADF
                     FEVAESTINNWKIEYPEFLESIKKGKLLADANVANSLYKRALGYEAPDIDIRVIDNQI
                     VETPLIKHYPPDPTSAIFWLKNRQPDKWRDKQIQEVSGTVTSQNINLSNDEFRAIAKE
                     LLEKV"
     gene            524..1903
                     /gene="terL"
                     /locus_tag="PWY43_001005"
     CDS             524..1903
                     /gene="terL"
                     /locus_tag="PWY43_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL2135896.1"
                     /translation="MFQFSAAQKIVAAELARTDLYFFTRYMFQQKRGYQWLQALHHEL
                     ICAALMRVFRGECKRLIINIPPRYSKTEIAIVNFVAWCFGQVPDCEFIHASYSSTLAV
                     NNSSNIRSLIQHEAYQEIFPNVTLDSEAKNHWTTSQGGAFYATGAGGTITGFGAGKER
                     AGFGGAILIDDPHKADEARSEVMRQNVIDWFQNTVESRKNSPENTPIIVIMQRLHEND
                     LAGWLLNGGNGEEWELLCLPAIQKDGSALWPEKHSIEQLRQMEQAAPYMFAGQYMQRP
                     APLDGGVFKPTQMPIIEALPVGKIKWVRGWDLGATVGGDPTAGVKLGKCEDGSLIIAD
                     IAHGDLGPDERDNMIRNTAALDGKSVRISLPQDPGQAGKTQALYLTRMLQGYPVKTSP
                     ESGDKLTRAEPFASQVNVGNVKMLKGAWNEGLINEMRLFPNGNHDDRIDACSRAYAEL
                     LAKGSSYFG"
     gene            1971..3482
                     /locus_tag="PWY43_001006"
     CDS             1971..3482
                     /locus_tag="PWY43_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDL2135897.1"
                     /translation="MFGWFKKEEPKQEIEEPKSYWANGKFYRGQPSKLFLDVLANIVQ
                     KNNEINTALSVGQDGDSESRLKMSVRNPEAISVALADWYASKAFIGYQMCAILSQNWL
                     VGKACAVPARDATRNGFDVVSINGDEISDETIKLLQRFDKKYRIRWHCEEFVRLGRVF
                     GMRIALFDIESSDPEFYEKPFNLDGVEPNSYKGIIQIDPYWCVPILVGGELNNPASQH
                     FYEPTYWQINGKKYHRSHLMIFRNDEVPDILKPVYMYGGISTPQKIMERVYSAERTTD
                     ESLGLVTSKRTTVWQTNMDAFMADFDGNREKIQAWIATRDNYGIKVGDKDDDQFSQYD
                     TTLSDLDEVIMTNYQIVAAAANVPVTKLLGTSPKGFSTGAEEAKNYHQELESIQEHDL
                     TEFVERHHQLVMKSFGNEVEDTTINWRPVDSPTAKELAELNKLKADTYSTLIMAGVID
                     GADARTVLVKDREAGFNDLGNVSEDLDLTEEEIALLNSVNVEQLQNAPEQHAD"
     gene            3457..4287
                     /locus_tag="PWY43_001007"
     CDS             3457..4287
                     /locus_tag="PWY43_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL2135898.1"
                     /translation="MHQNNTQIEGKPLIANASTGERYAKSIKRLMRAMHKEALNGIQS
                     CFEAYAEDDDLPKNGSIVAQLRILFSRLLSKYNPIFAILAKKTTARMVQETLKHSTAT
                     IKMSLKEVGKDLTVNPDFMDERLKEITQSGTLEAVSLIKTLPEKYLGDVQKVLTHSIS
                     TKGKGFAELKPFLQKLYKGNERKAELVALDQTRKVYHNVQAHKLQKLGVKKFKWLHSG
                     GGREPRALHVKLSGRVFSFDDPPYIGDMYGEKVYGLPGHLPNCACSMKPVFDFEVEND
                     "
     gene            4340..5530
                     /locus_tag="PWY43_001008"
     CDS             4340..5530
                     /locus_tag="PWY43_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135899.1"
                     /translation="MDDKWITVKPNGEKGKGSPVKIDDEGRIIAGMGGKFKGEKINEI
                     RKSFNGPKTPQRETLETKKQLEKNVQTVTRKTEEKIKSADVPELRSLSKTLENISRSA
                     VATLNNESVQQEYLKLKGKVDKRIAELENLSKPLSIAEKIKALSRTQAKVERVEKSKP
                     FSAQIKEIYSHQRNTEGARDFSKISETDTVNFAKALQKINKQGGYSAQKFNEIATFAW
                     QRESFDVRQLGLNKVADDYVKAGLMKAVDPDRHIYGLTEQGAYIANSVANIVEKEKAN
                     NQPKKKGKSIPALPAGLTPVKIVNEEQVSSWGISTNSNPSASGSNYVKSEATIKKSNA
                     TYLNVPYAEKDGAKALGAKWDKFKKKWYLPAGKEMPEGLKKYATDGFSISERLDILLQ
                     RMKQ"
     gene            5527..6783
                     /locus_tag="PWY43_001009"
     CDS             5527..6783
                     /locus_tag="PWY43_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDL2135900.1"
                     /translation="MTLAQDKKDIDTNGWFEVKDNPLSKEGVFLYRGSQIRLPDGTQP
                     PDLNQLYPVYRPAEELEKSIPSFRLIPWVDEHTMLGGEEMGMTPAEKKGVNGVIGEDV
                     YFKNGILFGNIKVFSERFARQIENGKKELSLGYRCSYEHSPGVWNGQKYDYIQRDLRG
                     NHLALVEKGRMGADVRVMDSSDVEPNHEQFIFTCDSIMEKKQMTLKELLENAAKQMNE
                     TSSLLSKAAELAEGGAEDDEPTPEQEQEPEMPSQESDTEPEFNEEGQDDEGDVLSKLL
                     DVVERLDARIAKLEGGSGQDDDDMPPEETGKDDDNKQSNEPNAMDAAEITSRAIKAIG
                     ERDALYKKVSAFTGSFNVSAMDSADAVAKYACSKLGLKAPKGQEMAMITGYMANRTPA
                     NKQHQVSVMDGLPASGKSFLDGQINQ"
     gene            6795..7304
                     /locus_tag="PWY43_001010"
     CDS             6795..7304
                     /locus_tag="PWY43_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135901.1"
                     /translation="MTFQQKINIEQGFGVPGDIHLDSPMRAESLVMNSEGSQKNIIGY
                     AFTKDAATNEAKVGGEIASGRVFAGILANSKEYPLHGTEKGTLEPTLALPDHSRGDFV
                     TMGDVVVRVKSACKIGDFVVYDATTGELSTVADKGNLGGKQLVPNAVIYRYPVTNSSG
                     GLTVVRLTN"
     gene            7316..8446
                     /locus_tag="PWY43_001011"
     CDS             7316..8446
                     /locus_tag="PWY43_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HDL2135902.1"
                     /translation="MTKSIQKGHISGRRFATQMAAQKPVIAMDSENVKEFAALAKIGI
                     GFSQDYLNKAPQAWAMDDVQGGVFTGSTGAPIQFLQAWLPGFVHAATAPRKIDELIGM
                     TTVGEWHDEEVVQGVLEATGNAVPYGDLTPIPLANWNVEFARRTVIRFEQGIAVGKLE
                     SARAGAMRLDSAAAKRVAATNALHIERNKVGFYGYNDGNSRTYGFLNDPALLPYNTVA
                     TGKAGGTKWATKTFLEITNDITIAFSQLAAQSNGIIEPKTTPTVLALPTGADVYLGVV
                     SDMGVSVASWLKDTYPNCRVATAPQLVKANGGVDVLYLYAEEVYGDSSDDDNRVWVQA
                     VPAQFMALGAYPEGKIVKEDYTNATAGVMCKRPYAVYRASGI"
     gene            8516..8890
                     /locus_tag="PWY43_001012"
     CDS             8516..8890
                     /locus_tag="PWY43_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135903.1"
                     /translation="MPYIYSTLTCDNAYTIYSDGANGLKVPQGSVIVKGGTGIANDRL
                     ITPLGIITSVSEEELKQLKANPIFKQHLENGFIVVQEDGKAQDAEKVAADMSLDNKDA
                     PVTPSDYQAENAGTKVKNNKGK"
     gene            8893..9324
                     /locus_tag="PWY43_001013"
     CDS             8893..9324
                     /locus_tag="PWY43_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HDL2135904.1"
                     /translation="MNITDFRTMFPVFDDLDDPTIELWAEVAEEHLKASWVLNGKRLE
                     IAMRLMTAHLLHTNNVVINADGTAQQNSSGGGIVASASEGSVSVSFATPPTKNAWQHW
                     LAGSPYGLQLWALLKQWSAGGFYIGGLPERKAVRKVGGVFV"
     gene            9321..9926
                     /locus_tag="PWY43_001014"
     CDS             9321..9926
                     /locus_tag="PWY43_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135905.1"
                     /translation="MSAKFKAALEARIKAVKDANKKVVKVGIVGHQRYDDGTPVAYVA
                     AIHEYGGIFTVPARQATIYRQIDKNGEWTQKNGSRFVSKKKSNFATEVTIPAHTITIK
                     PRPFFRPAFSNNRKKWQKEISNLLKQGHSVEEALELVGSVAAGDVFEALSKVETPKLA
                     ESTIKAKEKRYKSKSDYRPDKPLFASGLLSESISYVVVDKE"
     gene            9929..10633
                     /locus_tag="PWY43_001015"
     CDS             9929..10633
                     /locus_tag="PWY43_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135906.1"
                     /translation="MQLTQSHIERINRLWLAMSFDSKEWDESKHPRAANGQFGKDGSQ
                     SAMKSVKANIGRGRSAMNTAIAEKRTVHRAMYHNELGWIDFEWGDTGKIKASGKTKGA
                     MGISHIIESRMRKDNLSYEQATKMLTKNVVEAIAKGKTVDKFEKGNVTALKIDHEKCR
                     VSLRKSKGSNAWIITAFELFEDAGSKGDGKTTATEHQSYSARIDAGASSKNSITPRSI
                     SNQVKKRINSLFQRNR"
     gene            10641..11021
                     /locus_tag="PWY43_001016"
     CDS             10641..11021
                     /locus_tag="PWY43_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135907.1"
                     /translation="MNLRQIANRAITTVNPNIPAVLKLNAGTETDDTGKRVAKFDEIN
                     VKIQPQSLSTNDLQLFDSLAQQGQLLNVYISGQIHALRRLSQQGADKLVFKAFGEKTE
                     SEWLIKSVAESFPNWCKVVIWRQK"
     gene            11006..11512
                     /locus_tag="PWY43_001017"
     CDS             11006..11512
                     /locus_tag="PWY43_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135908.1"
                     /translation="MAAEVTLNTTHNDIYREVRAYLLGLFHLEGDQVIRGYSNNAPLP
                     NPPFILMNIIHEQALSTNAYDYSIDEGNVEVMQSLEIQMQLDFYGETSGEISRKFCTL
                     WRNLYACERLERCQPLHCDEPKYLPFTNEASEYEERYMVTAYLTYNPVITHEQDFITN
                     PRISIHSL"
     gene            11528..13006
                     /locus_tag="PWY43_001018"
     CDS             11528..13006
                     /locus_tag="PWY43_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HDL2135909.1"
                     /translation="MFQSIPASRIVNISPAVLSAGGSPLSMNAVFLSKNQNLPTAQAV
                     PFATADAVGEYFGFASEEYQAAAVYFKGFDNSTIKPGTLFFFAYNEAAEGAFLVGNSV
                     KGLSLEELKTVSGSLTINIDGVEKTASSINLRSSKSFSQAAETISSALGDVTVSFDPQ
                     LQSFKISSGTTGSSSAITFATGDVADKLGLSQSVGAVISQGSAETSPTETMQQVIKST
                     LNWATFTTVFEPSLDDKKAFAKWSNDQDQRFLYVAWGKEAGAVQAGNNTCFGAWLKES
                     AYNGTVAIYGGLDKAAFVCGATASIDFTERQGRITYKFKGQAGLTADVTDATVAQILE
                     DNGYNYYGAWATANDRFLFLSPGQIAGKWKWIDAYINQIRLNSQLQLALITLLTSAKS
                     VPYNALGVALQRAACQDPIQEALDFGSIQIGVALSEQQKALINKDTGMDTAAQIEAQG
                     YSLYIGKTSAQVRGKRQSMPMKLWYTDGGSVHAINLASINVQ"
     gene            13064..13507
                     /locus_tag="PWY43_001019"
     CDS             13064..13507
                     /locus_tag="PWY43_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135910.1"
                     /translation="MRTNIDKTLTAANSILLVRAKGYNKSWVKMEGYAADNAFDFGQG
                     AIGETVIGVDGVQSGGFTPYEVDLNIQLQANSPSRSFFDGVINHINNNQETVPFEFSC
                     EIPSIGKRYSATGFLVNIPGGTNAKKLLESATYSFKVVNNGAEDI"
     gene            13642..14163
                     /locus_tag="PWY43_001020"
     CDS             13642..14163
                     /locus_tag="PWY43_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HDL2135911.1"
                     /translation="MKALKAKFFGSDILVINQNDKPYVPMKQVAENIGLVWHAQFERL
                     QRNEVLSQGIRVIRIPSNGGEQEAVCLPLHYLNGWLFGVNPSRVNPEIKEKLIRYQTE
                     CYEVLWDYWTTGIAKWEDIRQQRENLEENESESKKRGSEAGRALQKRKVEKHTYEIGI
                     ERLDRMEQLLLEF"
     gene            14231..14635
                     /locus_tag="PWY43_001021"
     CDS             14231..14635
                     /locus_tag="PWY43_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135912.1"
                     /translation="MALKTKKITIEKGRDAGTTFLITEMPIAKADKWASRALLALASG
                     MEGIPSASEGMLGMARVALAAFKQIPEEKALPLLDELLDCVEIVMDSGSTRPIDLAIG
                     DVSDITTLWKLRKEAFALHIDFLDLELTPISE"
     gene            14662..14808
                     /locus_tag="PWY43_001022"
     CDS             14662..14808
                     /locus_tag="PWY43_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135913.1"
                     /translation="MNVSSVVGVVVSSKLATLHELQTIYGLEDALDLIEVFSVDTYNH
                     RDNK"
     gene            14808..17477
                     /locus_tag="PWY43_001023"
     CDS             14808..17477
                     /locus_tag="PWY43_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL2135914.1"
                     /translation="MSEVIDTLAIEIAANDSFTAVAKPLLSLLERLEGAVNKNTEALD
                     NHGKAAESAAKGTKKLNTEQGKGEKSTKKSTKALQEQEKQIKKNEKVAKNLLQAIGGF
                     TKAIGALGTMILAGVGLDRLVLDTAKANKELAMTSKNLGMTSQSLAAWRGAAELSGGS
                     AQGLTGYLNNLSAGLTRLTVQGDASVTQFFSQLGINLLDGSQKAKKLEDIMLELADKF
                     SSMDRVKAFGIAQQMGIDEGTFEMLAQGRQGLEEHLQKTAKIYKSNQQDLETARKLTA
                     ATAYLNQQFEGLKLMIANAAMPVLLKIADVTNAFFEYLQKNENLVKGVFYGIAGAISI
                     ALIPMLISGASAALAFIAPFLPMILIVAGLAAAIGLLYDDYEKWANGGTSLFNWGLFK
                     KWIFDADFTVGNLKNAFADLLTGYKSWEDAVNAAKSWLELKGFTKDGKMSIDSLATGF
                     KNVAKDIYNSLLPALDKVLNVINLLLDGEFVAAWDAAKELGKDAFNAAVDFVGDNIVG
                     DALKGVGGIIDAGVTNIEEAVNTGKKYWNGEITGSEALKEVTGNFAKNAEKAVDEVVN
                     FADNAITRVENGFVRFVNIWGGFDPDEVGPTGNENKLMDSKKGFSKGNFVFEGQQSVK
                     GLTQEETGALAAQMVKRESGGNLRAENQYGYLGLYQFGAAALVDAGLIDDAKYRAAVR
                     KHGKGLSNGSDADVHKAFLADSSNWTIKGGREAFLSNKAIQDNAIVALMNKNVGYLGS
                     TYQGSAEHKAGLLMAAHLKGAGGAKAFANSGIDSSDGNGTKISDYYNTGRKAIQLAKQ
                     GKSIQSKPINLNTPVGGYAVANALKANQHTFTTMQNLAQPQNISNNQKTEVAFNGGIH
                     INSTAGTLGGTAQDFVNGVQNRVNVMQFNTGLS"
     gene            17477..18139
                     /locus_tag="PWY43_001024"
     CDS             17477..18139
                     /locus_tag="PWY43_001024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135915.1"
                     /translation="MKRAFILLPIIFSSNAFADEMLFSCLTEKNNIISLINKGDMVEY
                     QVASAKTDKITFSFEQPIGNINLSSSVEQGVTQKVYQISNNGYRYDLVVWEKENNKLP
                     VGVEAKDPSGKTVSTNRCKEFIDFINSDTDNKAEENTSSNEPVSNQVYEEGMYRVGRD
                     IPAGEYKLTTTNKERGYFTIYSSSERDSVIAVDTFKNSTYLTVKEGQYLELRNSNAQL
                     VE"
     gene            18162..18833
                     /locus_tag="PWY43_001025"
     CDS             18162..18833
                     /locus_tag="PWY43_001025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL2135916.1"
                     /translation="MFKFKKQSNQSSSNQENNKISIADELDKLVSLKEKGILSELEFE
                     KEKDKLLNGAKYQAQQPTFIIQNSASSSSSASATAVVKRSGCLSSIIKALLALFVIFL
                     ILDFIAWSSGNKTASEVKAKQLISDKQCNQLIKDLIYHKHAQDICLVEAKLGEFNSNL
                     NGDSFAEYSTRNGCPQLPENVAYKLQEETSLNFINALKSSPSKVEFCKKELPYFKKLV
                     QKYAS"
     gene            18985..19164
                     /locus_tag="PWY43_001026"
     CDS             18985..19164
                     /locus_tag="PWY43_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135917.1"
                     /translation="MSNYSKTKLKIHLKKGLEMETNASPIMRGAIAISIVLIALGVFA
                     LCVTPLANVLIEMIK"
     gene            19449..>19939
                     /locus_tag="PWY43_001027"
     CDS             19449..>19939
                     /locus_tag="PWY43_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HDL2135918.1"
                     /translation="MSNQITTQTLSFYGSDLITLKVEDVIYTAVRPIVDALGLSWGSQ
                     QRKLENSKEKFSCTHMKTTGSDGKTYEMLCMPIKKLNGWLFSINPEKVRVDLKEKVIC
                     YQEECFEALYNYWHLGKAERLQNSAENQTALPEPTLTAEEQNMLQNAVKATHERTKLS
                     YGE"
BASE COUNT         6383 a         3706 c         4671 g         5179 t
ORIGIN      
        1 atgaagtaaa ggaatttaac ccaatggcta aaaaaggcga ggttaaagcc acgtctaagt
       61 cacgtggtca acctacaaaa tataaaccag agtacgttac tcaagttgaa aaattatgtc
      121 tgcttggcgc aacggacaaa gatatagccg atttttttga agtggctgaa tctactatta
      181 ataactggaa aattgaatat cctgaattct tggagtccat aaaaaaagga aagttattag
      241 ctgatgcgaa tgtagccaac agcctttata aaagagcatt gggctatgag gctccagata
      301 ttgatattcg agtgattgat aatcaaattg ttgaaacgcc tttgattaag cattatccgc
      361 cagatccaac atcagccatt ttttggctca aaaataggca gcctgataaa tggcgtgata
      421 agcaaattca agaggtttca gggactgtta ccagccaaaa catcaaccta tccaacgatg
      481 aatttagggc gattgccaaa gaattattag aaaaggtgta gttatgtttc agtttagtgc
      541 agcacaaaaa atcgtggcgg ctgaactggc aagaaccgat ctctattttt tcactcgcta
      601 tatgtttcag caaaagcggg gttatcaatg gctacaagca ctacatcacg aattgatttg
      661 tgccgcgtta atgcgagtgt ttcgtggcga gtgtaagcgg ttaatcatca acattccacc
      721 acgttattca aagacagaaa ttgcgattgt gaatttcgtg gcgtggtgct ttggacaagt
      781 gccggactgc gagtttattc acgctagtta ttcatctacg cttgcggtta ataatagttc
      841 aaatatccgc tcacttattc aacacgaagc ctatcaagaa atcttcccga atgtcacact
      901 tgatagtgaa gcaaaaaacc attggacaac ctcacaaggc ggcgcgttct atgccaccgg
      961 tgcaggcggt acgataaccg gttttggtgc aggtaaagag cgagccggtt ttggcggagc
     1021 aattctgata gacgatccgc ataaagctga tgaggctcgc tccgaagtaa tgcggcaaaa
     1081 tgtgattgat tggtttcaga ataccgttga gagccgtaag aacagtccgg aaaacacgcc
     1141 gattatcgtg attatgcaac gcctgcacga gaacgattta gccggttggt tattaaacgg
     1201 cggaaacggt gaagagtggg aattgttgtg tttgcctgct attcagaaag acggttcagc
     1261 attatggccg gagaagcaca gtattgagca acttcgccaa atggaacaag cagctcccta
     1321 tatgtttgcc ggtcaatata tgcaacgccc agcaccactt gacggcggtg tatttaagcc
     1381 aacgcaaatg ccgatcattg aagcgttgcc ggtaggtaaa atcaaatggg tgcgtggctg
     1441 ggacttgggg gcaacagtgg gcggagatcc gaccgccggt gttaagctcg gcaaatgcga
     1501 agatggttcg ctgatcattg cagatattgc acacggcgat ttagggccgg acgaacggga
     1561 taatatgatc cgcaacacgg cagccttaga cgggaaaagt gtgcgtatta gcttaccaca
     1621 agacccgggg caagctggga aaactcaagc cctgtattta acccgaatgc tacaaggcta
     1681 tcccgttaaa accagccctg agagtggcga caagctcacc cgagccgagc cgtttgcctc
     1741 acaagtgaat gtaggtaacg tgaaaatgct taaaggggcg tggaatgagg gattaatcaa
     1801 tgaaatgcgg ttattcccga acggcaatca cgatgaccgc atagacgctt gttctcgtgc
     1861 ttatgcagag ctgttggcga aagggtcttc atattttggt taattgcaaa attttattga
     1921 aatccaaccg cttgtagcaa tacaggcggt tttttattac agagagaaat atgtttggtt
     1981 ggtttaaaaa agaagagcca aaacaagaaa tagaagagcc taaatcctat tgggctaacg
     2041 gtaaattcta tcgtgggcaa ccgtctaaac tgtttcttga tgtgttggcg aatattgttc
     2101 agaaaaataa tgaaatcaat accgcacttt ctgtcggtca agatggcgat agtgaatcac
     2161 gcttgaagat gtcggtgcga aatcccgaag cgattagcgt tgccttggcg gattggtatg
     2221 cttcaaaggc atttatcggg tatcagatgt gtgcgatttt gtcgcaaaac tggcttgtag
     2281 gtaaagcctg tgctgtaccg gcaagagatg ctaccagaaa cggttttgac gtggtgtcta
     2341 tcaatggtga tgaaatctca gacgaaacaa tcaagctgct tcaacgcttt gataaaaaat
     2401 accgcatccg ctggcattgt gaagaatttg tacgcttagg gcgtgtgttt ggtatgcgga
     2461 ttgcattgtt tgacattgag tcaagcgatc ctgaatttta tgaaaaaccg tttaatttag
     2521 acggtgtaga accaaacagc tacaaaggga ttattcaaat cgatccttac tggtgtgtgc
     2581 ctatcttggt cggcggtgag ttgaataacc cggcaagtca gcatttctac gaaccgacct
     2641 attggcaaat caacggtaaa aaataccacc gttcgcactt gatgattttc cgcaatgatg
     2701 aagtgccgga tattttaaag cctgtctata tgtatggcgg tatttcaacg ccgcagaaga
     2761 ttatggaacg ggtttacagt gcggagcgaa caaccgatga atcgttggga ttagttacct
     2821 caaaacgcac aaccgtatgg caaaccaata tggacgcttt tatggcggac tttgacggca
     2881 atcgggaaaa aattcaagcg tggattgcaa ctcgtgataa ctacggtatt aaggttggcg
     2941 ataaagacga tgaccagttc agccagtacg acacgacatt gagcgacctt gatgaagtga
     3001 ttatgactaa ctatcaaata gtagccgctg ctgcaaatgt gccggtaaca aaattacttg
     3061 gcacatcacc gaaaggattt tcgactggtg cggaagaggc gaagaactat caccaagagt
     3121 tagagagtat tcaagaacac gatttgaccg agtttgtaga acgtcatcat cagcttgtga
     3181 tgaaatcatt tggtaatgag gtagaagata ccacaattaa ctggcgaccg gttgattctc
     3241 caacagcaaa agagctggca gagttgaata agctgaaagc cgatacttac tctacgctaa
     3301 ttatggctgg tgtaattgat ggtgctgacg ctcgaactgt gttagtaaaa gaccgagaag
     3361 ccggttttaa tgatttaggc aatgtcagcg aagatttaga tttaactgaa gaagaaatcg
     3421 cattacttaa ttcggtcaat gtggagcaac tgcaaaatgc accagaacaa cacgcagatt
     3481 gaaggcaagc cactaatagc caatgcttca acaggtgagc gttatgccaa aagcattaag
     3541 cggttaatgc gagcaatgca caaagaggcg ttaaatggta ttcaatcttg ctttgaggct
     3601 tatgccgaag atgatgacct gccaaaaaac ggctcgattg tggcgcagtt gcggattttg
     3661 tttagtcggc ttttaagtaa gtataatccg atttttgcaa ttttagccaa aaaaacaacc
     3721 gctagaatgg tgcaggagac gctcaaacac tctactgcaa ccattaagat gagcttgaaa
     3781 gaggtcggca aagatttaac ggttaatcct gattttatgg atgagcgact gaaagaaatt
     3841 acccaatccg gcacgcttga agcggtatcg ctaataaaaa ccttgcccga gaaatacttg
     3901 ggcgatgtac aaaaggtgct gacacactcg atttcaacaa aggggaaagg ctttgcggaa
     3961 ttaaaaccgt ttttgcaaaa actctacaaa gggaatgagc gaaaagcgga actggttgct
     4021 ctcgatcaaa ctcgtaaggt ttatcacaat gttcaggctc ataaattgca aaaattgggt
     4081 gtaaaaaaat tcaaatggct acattcaggc ggtgggcgtg agccgagggc cttacacgtt
     4141 aaactaagcg gtcgggtatt ttcgtttgat gacccgcctt atattggcga tatgtacggc
     4201 gaaaaggttt atggtttgcc cggtcatctg ccaaattgtg cgtgcagtat gaagcctgtc
     4261 ttcgattttg aggtagaaaa tgattaacat cacacaatca catagaaacc gtattgaaag
     4321 actgttttcg gttgtcgcaa tggacgataa atggatcaca gttaagccta acggcgaaaa
     4381 aggtaaaggc tcaccggtca aaattgacga cgaaggtcga atcattgccg gtatgggtgg
     4441 caaattcaaa ggcgagaaaa ttaacgaaat tcgtaagagt tttaatgggc caaaaacgcc
     4501 acagcgagaa acattagaaa caaagaaaca acttgaaaag aacgttcaga cagtaactcg
     4561 taaaactgag gaaaagatta agagtgcgga tgtgcctgaa ctacgaagtt tatctaagac
     4621 gttagaaaat atctctcgaa gtgccgtagc gactttaaac aatgaaagcg tacagcaaga
     4681 atacctgaag ttgaaaggca aggttgataa aagaattgct gagttagaaa atttatctaa
     4741 accactaagt atagcagaaa aaattaaggc tttatcacgt actcaagcaa aggtagaacg
     4801 ggtggaaaag tcaaaacctt tctctgctca aattaaagag atttattccc atcaacgaaa
     4861 tactgaagga gcaagagatt tttcaaaaat aagtgaaact gataccgtta attttgcaaa
     4921 agcattgcaa aagattaaca aacaaggtgg atattcagct cagaaattta atgaaatcgc
     4981 aacatttgcg tggcaaagag aaagttttga tgtaaggcaa ttaggcttaa ataaggttgc
     5041 tgatgattat gtcaaagcag gtttgatgaa agcagtcgat cctgataggc atatttatgg
     5101 actaacagag caaggtgcct atattgcaaa tagtgttgct aatattgttg aaaaggaaaa
     5161 agcgaacaat caacctaaga aaaaaggtaa aagcattccg gctcttcctg ctgggttaac
     5221 tcctgtgaaa attgtaaatg aggagcaggt aagctcttgg ggaatatcaa caaacagtaa
     5281 tccgtcagct tctgggagta attatgttaa atcagaagct acaataaaaa aatctaatgc
     5341 tacttactta aatgtcccat acgccgaaaa agacggggca aaagctcttg gtgcaaaatg
     5401 ggataagttt aagaagaaat ggtatttacc ggcaggtaaa gaaatgccgg aagggttaaa
     5461 gaaatatgcg actgatggtt tttcaatctc cgaacgctta gatattttgt tgcaaaggat
     5521 gaaacaatga cactcgcaca agacaaaaaa gatattgaca caaacggttg gtttgaagtt
     5581 aaagacaacc ccctcagcaa agagggggtt tttctttatc ggggttcgca aattcgatta
     5641 cctgacggca cacaaccacc ggatttaaac caactttacc ctgtttaccg ccctgctgag
     5701 gagttggaaa agtcaatccc tagctttcgc ttaattccgt gggtggacga acacacgatg
     5761 ttaggcggtg aggaaatggg aatgacaccg gctgaaaaga aaggcgtaaa tggggtaatt
     5821 ggggaagacg tttattttaa aaacggcata ttattcggaa acattaaggt gttttcagaa
     5881 cggtttgccc gacaaatcga gaacggcaag aaagagcttt ctctcggtta tcggtgcagt
     5941 tatgaacata gtcccggcgt ttggaatggg cagaaatatg actatatcca gcgggattta
     6001 aggggcaatc atttagcctt agtcgaaaaa ggcagaatgg gggcagatgt tcgagttatg
     6061 gatagcagtg atgttgagcc aaaccacgaa cagtttattt ttacttgtga ttccataatg
     6121 gagaaaaaac aaatgacatt aaaagaattg ctggaaaacg cagcaaaaca gatgaatgaa
     6181 acctcctcac ttttgagcaa agccgcagaa ttggcggaag gtggtgcgga agatgacgag
     6241 ccaacgcctg agcaggagca agagcctgaa atgccaagtc aagagtctga tactgaacct
     6301 gaatttaatg aagaggggca agacgatgaa ggcgatgtgt taagcaaatt gcttgatgta
     6361 gtcgagcgtt tagatgcccg tattgcgaaa ttagaaggtg gcagcggtca agatgatgac
     6421 gatatgccac cggaagaaac tggtaaggac gatgacaaca agcagtcaaa tgaaccaaat
     6481 gcaatggatg cggcggaaat taccagtcgt gctatcaaag ctattggtga acgagatgct
     6541 ctgtataaga aagtctctgc atttactggg tcgtttaatg tatcagcaat ggactcagcc
     6601 gatgcagtag ctaaatatgc ttgctcgaaa ttaggcttaa aagccccaaa agggcaagaa
     6661 atggcgatga ttaccggtta tatggcaaac cgtacgccgg caaataaaca gcaccaagtg
     6721 agtgtaatgg acggcttacc ggcaagcggt aaatcattcc ttgatggtca aatcaatcaa
     6781 taaggtaggt agaaatgaca tttcaacaaa aaatcaatat tgaacagggc tttggtgtac
     6841 cgggtgatat tcatcttgat tccccaatgc gtgcagaaag ccttgtaatg aactcagagg
     6901 gttcacaaaa aaacattatt ggttacgctt tcactaaaga cgctgcaacc aatgaggcaa
     6961 aagtaggcgg tgaaattgca agtggtcggg tgtttgcagg tattttggca aactccaaag
     7021 aatacccatt acacggtaca gaaaaaggca cattagagcc gacattagca ttgccagatc
     7081 acagtcgtgg tgatttcgtt acgatgggtg atgtcgtcgt tcgtgtgaaa tctgcgtgta
     7141 agatcggcga ttttgttgtg tacgatgcga ctaccggcga actttctacc gttgcagata
     7201 aaggcaattt aggtggtaaa cagttagtgc cgaatgcggt gatttatcgc tatccggtaa
     7261 ccaatagtag cggcggttta accgtagttc gtttaacaaa ttaaggaaat gatagatgac
     7321 taaatcaatt caaaaagggc atatcagcgg tcgccgtttt gcaactcaaa tggcggcaca
     7381 gaaaccggtg attgcaatgg actctgaaaa tgttaaggag tttgcagcac tggcaaaaat
     7441 cggtattggc tttagccaag actacttaaa taaagcgcca caagcctggg cgatggatga
     7501 tgtgcaaggc ggagtattta caggtagcac cggtgcaccg attcaattct tgcaagcgtg
     7561 gctaccgggt tttgttcacg ctgcaactgc accccgtaaa atcgatgagt taatcggtat
     7621 gacgaccgtg ggcgagtggc acgatgaaga agtcgttcaa ggtgtgttag aagccacagg
     7681 caatgcagta ccatacggcg acttaacgcc aattccgtta gcaaactgga acgtagaatt
     7741 tgcacgccgt accgtaattc gctttgaaca aggcattgcg gtaggtaaat tagaatctgc
     7801 ccgtgccggt gcaatgcgtt tagacagtgc agctgcaaaa cgtgtggcgg caaccaatgc
     7861 gttacatatt gagcgtaaca aagtcggttt ctacggctat aacgatggca actctcgcac
     7921 ctacggtttc ttaaacgatc cggcattatt gccatataac accgttgcaa ccggcaaagc
     7981 aggtggcaca aaatgggcaa ctaagacgtt cttagaaatt actaacgaca tcaccattgc
     8041 gttttcgcaa ttagccgccc aatcaaacgg cattattgag ccaaaaacta cgccaacggt
     8101 attagcatta ccaaccggtg cggatgtata tcttggtgtt gtgtctgata tgggcgtaag
     8161 cgtagcttcg tggttaaaag acacttaccc aaattgtcgt gtagcaactg ctccgcaatt
     8221 agtaaaagcg aatggtggcg ttgatgtgtt atatctctac gctgaagaag tttatggcga
     8281 cagctcggac gatgataatc gtgtttgggt gcaagccgta ccggcacagt ttatggctct
     8341 cggtgcttac cctgaaggca aaattgtgaa ggaagattac accaacgcaa ctgccggtgt
     8401 aatgtgtaaa cgcccttatg ctgtttatcg agcttccggc atttaatgca aaaaaacaac
     8461 aaaaaacgac cgctctttat gagcggtttt ttattaccta aacaggagaa acataatgcc
     8521 ttatatctat tcaaccttaa cttgtgataa tgcttatacc atttattcag acggtgcaaa
     8581 cggcttaaaa gtgccacaag gtagcgtaat tgttaaaggt ggcacaggga ttgcgaatga
     8641 ccgtttaatc acaccattag ggattatcac ttcggtatcg gaagaagaac ttaaacaact
     8701 caaagcaaac ccgattttta aacagcattt agaaaatggt tttattgtgg tgcaggaaga
     8761 cggcaaagca caagatgctg aaaaagtggc tgcggatatg tcgttagaca ataaagatgc
     8821 accggttacg ccgtctgact atcaagctga gaatgccggc accaaagtaa aaaacaacaa
     8881 aggtaagtaa caatgaacat caccgacttt cgcacaatgt ttcctgtatt tgatgattta
     8941 gacgatccaa caatcgagct ttgggcggag gtggcggaag aacatttaaa agcaagttgg
     9001 gtattaaacg gcaagcggtt agagattgca atgcgattaa tgaccgctca tttactccat
     9061 accaacaatg ttgtgataaa tgcagacggc acagcacaac aaaatagcag cggtggtggc
     9121 attgttgctt ctgcttccga gggtagcgta agcgtttcat tcgcaacacc gccgactaaa
     9181 aacgcttggc agcattggct tgccggttcg ccctatggct tgcagttgtg ggcgttgcta
     9241 aaacaatggt ctgcaggtgg tttttatatc ggcggtttgc cggaacgtaa agcagtgcgg
     9301 aaagtcggtg gggtgtttgt gtgagtgcaa aatttaaagc agcgttggag gcacggatta
     9361 aagccgtgaa agacgcaaat aaaaaggtgg ttaaagtcgg tattgttgga catcaacgct
     9421 atgatgatgg cacgcctgtt gcttatgttg cggcgattca cgagtacggc ggaatattta
     9481 ctgttcctgc cagacaggcg actatttatc gacaaataga taaaaatggc gaatggacac
     9541 agaaaaatgg tagtcgcttt gtcagcaaga aaaaatcgaa ttttgcgaca gaagtaacta
     9601 ttcctgctca tacaatcacc attaagccga gacctttttt tagacctgct ttctccaata
     9661 ataggaaaaa gtggcaaaaa gaaatttcaa acctattaaa gcaagggcat tctgtagaag
     9721 aggcattaga gcttgtcggt agtgttgccg ccggtgatgt atttgaggca ctaagcaaag
     9781 tagaaacacc caaattagct gagagtacaa ttaaagcgaa agaaaagcgt tataaatcta
     9841 aatcagatta ccgcccagac aaacctcttt ttgcaagtgg cttgctttca gaatccatct
     9901 catatgttgt tgtagataag gagtagttgt gcaactgaca caatcacaca ttgaacggat
     9961 taatcgctta tggctggcaa tgtcatttga cagcaaagag tgggacgaaa gcaaacaccc
    10021 gagggcggca aacgggcagt ttgggaaaga tggctcacaa tctgcaatga agtctgtgaa
    10081 agcgaatatc gggcgaggtc gctcagcgat gaataccgca atcgcagaaa aaagaactgt
    10141 tcatcgtgca atgtatcata acgaacttgg ctggattgat tttgagtggg gtgatactgg
    10201 aaaaattaaa gccagtggga aaaccaaagg tgctatgggt atatctcaca ttattgaatc
    10261 tcgtatgaga aaagataatt taagttatga acaagctacc aaaatgctga caaagaatgt
    10321 cgtggaagcc attgctaaag ggaagactgt tgataagttc gagaaaggaa acgtaacggc
    10381 acttaaaatc gaccacgaga aatgccgagt atctcttaga aaatcaaaag gcagtaatgc
    10441 ttggattatt actgcctttg agttatttga agatgcaggt agcaaggggg atggcaagac
    10501 taccgctact gaacatcaat cctattccgc tcgtattgat gcaggagctt caagcaaaaa
    10561 cagtataacc ccaagatcaa tttctaatca agtgaaaaaa cgaataaatt cgcttttcca
    10621 aagaaatagg taaatgaaca atgaacctac gccaaatcgc aaatcgtgcg atcaccacag
    10681 tcaatccgaa tattccggca gtattgaaac tcaatgccgg aacagaaacg gatgacaccg
    10741 gcaaaagagt ggcaaagttt gatgaaatca acgtcaaaat tcagcctcaa agcctttcaa
    10801 ccaatgattt acagcttttt gacagtctcg cccaacaggg tcagttgctt aatgtttata
    10861 tttcagggca gattcacgcc ctacgccgcc tttctcaaca gggagcggat aaattagtgt
    10921 ttaaggcttt tggcgaaaaa acggaatcag aatggctgat taaatccgtt gccgaaagtt
    10981 ttcctaactg gtgcaaggtg gtgatatggc ggcagaagta accttaaaca ccactcataa
    11041 tgatatttat cgagaagtcc gggcttattt gctcgggctt tttcatttag agggcgatca
    11101 ggttattcga ggctatagca ataacgcacc gctaccaaat ccgccgttta ttttgatgaa
    11161 tattattcac gaacaagcct tatccactaa tgcttatgat tacagcattg atgaaggcaa
    11221 tgtagaagtg atgcagagcc ttgaaattca gatgcaactt gacttttacg gcgagacaag
    11281 cggtgaaatt tcacggaaat tttgcacatt atggcggaat ttatacgcct gtgagcgatt
    11341 ggagcgttgc cagccgcttc attgtgacga accgaaatat ttgccgttta ccaatgaagc
    11401 cagcgaatac gaggagagat atatggtaac ggcatattta acctacaacc ctgtgatcac
    11461 tcacgaacaa gattttatta ccaacccaag aatttctatt cattctttat aggagaacaa
    11521 catagctatg tttcaatcta ttcctgcttc ccggattgtg aatatcagtc ctgcggtatt
    11581 aagtgccggt ggttcgccac tctcaatgaa tgccgtgttt ttaagcaaaa atcaaaatct
    11641 gccgactgct caagccgtac cgtttgcgac tgctgatgcg gtaggggaat acttcggctt
    11701 cgcctcagaa gagtatcaag cggcggcagt ttactttaaa ggctttgaca actcaaccat
    11761 caaacccggc acactgtttt tctttgccta caacgaggca gcagaagggg ctttcttggt
    11821 tggtaattca gtaaaagggt tgtcattgga agaattgaaa acggtttcag gctctcttac
    11881 tatcaatatt gatggtgtag agaagaccgc ttcgtcaatt aacctccgtt catcaaaaag
    11941 tttttcgcag gcggcagaaa ctatttcttc cgcactgggc gatgtaacgg tttcttttga
    12001 tccgcagttg caatcattca agatttcatc cggcaccaca ggttcatcat ctgctatcac
    12061 tttcgcaacc ggtgatgttg cagacaagtt aggcttatcg caatcagtcg gtgcggttat
    12121 ttcccaaggt agtgctgaaa catcgccgac tgaaacaatg cagcaggtga tcaaatccac
    12181 tttaaactgg gcgacattta ccaccgtatt tgagccaagt ttagacgaca aaaaagcctt
    12241 tgcgaaatgg agtaacgacc aagatcagcg tttcttgtat gttgcttggg gcaaagaagc
    12301 cggtgcggta caagccggta ataacacttg tttcggagcg tggttaaaag aatctgctta
    12361 taacggcaca gttgctattt atggcggact agataaagcc gcttttgtgt gtggtgcaac
    12421 cgcttctatt gatttcaccg aacgacaagg acgaattact tataaattca aagggcaagc
    12481 cggtttaact gccgatgtaa ctgatgccac tgttgcacag attttggaag ataacggcta
    12541 caactattat ggagcttggg caaccgcaaa cgaccgcttc ttgttcttgt cgccgggtca
    12601 gattgccggc aaatggaaat ggattgacgc ttacatcaat caaattcgcc tgaatagcca
    12661 attacaactt gccttgatta ccttgcttac ttcggcgaag tctgtgccat ataacgctct
    12721 cggcgtggct ttacaacgtg cggcttgtca agatccgatt caagaggcgt tagatttcgg
    12781 ctctatccaa attggtgtgg cattaagcga acagcaaaaa gccttaatca ataaagacac
    12841 cggaatggat accgccgccc aaattgaggc acaaggttat tcactctata ttggcaaaac
    12901 ttcggcacag gtgcgaggta aacgccagtc aatgccgatg aagttatggt acaccgacgg
    12961 cggcagtgtt cacgccatta accttgcttc tattaacgtt caataacagt aacaagcggt
    13021 caaattcgac cgcttttttg caaaaaaagg aaacgaataa attatgcgta caaatattga
    13081 taaaactctg accgccgcaa acagcattct acttgtgcgg gcaaagggtt ataacaaatc
    13141 gtgggtcaaa atggaaggat acgccgccga taatgccttt gacttcgggc aaggtgcaat
    13201 cggcgaaacc gtgatcggcg ttgatggtgt gcaatcaggc ggatttacgc cttatgaagt
    13261 cgatttaaat attcaacttc aagccaacag cccgagccgt tctttttttg atggtgtgat
    13321 taaccatatc aacaacaacc aagaaactgt gccgtttgaa ttctcgtgcg aaattccgtc
    13381 tatcggcaaa cgctattcgg caaccggctt tttagtgaac attccgggcg gtacgaatgc
    13441 gaagaaattg cttgaatctg cgacctattc attcaaagtc gtgaataacg gtgcagaaga
    13501 tatttaacct ttaatgttcc caaacagctt taaatcgtga gtttacgagc ctgtaataag
    13561 gtaggaatgt tgcggtaatt cacaacccga tcagaaatgg tcgggttttt tattatccaa
    13621 aattcataaa aggaaaaaat aatgaaagca ttaaaagcaa aattttttgg ttcagacatt
    13681 ttagttatca atcaaaatga taagccatat gtaccaatga agcaggtggc agaaaatatc
    13741 ggtttagttt ggcacgctca atttgagcga ttacaacgaa atgaagtact ttcacaaggt
    13801 attcgtgtta tacgaatacc ttcaaatggt ggtgagcaag aagcagtttg tttgccgtta
    13861 cattacctta atggctggct gtttggtgta aatccttctc gtgtaaatcc cgagatcaaa
    13921 gaaaagctga ttcgttatca aacagagtgt tacgaagtcc tatgggatta ttggacaacc
    13981 ggcattgcca aatgggagga catccgccaa caacgtgaaa acttagaaga aaatgaaagt
    14041 gaatcaaaaa agcgtggcag tgaggctggg cgagcgttgc aaaaacgaaa agtagaaaag
    14101 catacctatg aaattggcat agagagacta gatagaatgg aacagctgtt acttgaattt
    14161 taaagatata gggtaaattc agcaaatgga tctgttctta gatatttaac cacctcagag
    14221 gaataaaaaa atggctttaa aaacaaaaaa aatcacgatt gaaaagggac gagatgcagg
    14281 cacgacgttt ttgattacgg aaatgccgat tgccaaagcg gacaagtggg cttctcgtgc
    14341 attacttgcc ttggcgagtg gaatggaagg tatcccgtca gcttcggaag ggatgctggg
    14401 aatggcaaga gttgcactag cggcttttaa gcagattccg gaagaaaaag ccttaccgtt
    14461 attagatgaa ctattggatt gtgtagaaat cgtgatggat agcggctcaa ccagaccgat
    14521 tgatttagcg attggtgatg tgagtgacat cacaacgctt tggaaattgc gaaaagaggc
    14581 gtttgcgttg catattgatt ttttggatct cgaacttacc ccgatctcgg agtaagtaaa
    14641 gaaagcaatg ctgttgcgta tgtgaatgta agttcggttg ttggggttgt ggtaagtagt
    14701 aagctggcaa ccttgcacga attacagact atttatgggc tggaagatgc tctagatcta
    14761 attgaagtgt tcagcgtgga tacttataac catagggata ataaataatg tcagaagtta
    14821 ttgatacatt agcgatagaa attgcggcga atgatagttt tacggcagta gcaaagccgt
    14881 tattatccct attggaacgt ttagagggag cggtcaataa aaacaccgaa gccttagaca
    14941 accacgggaa agcggctgaa agtgccgcca aaggcactaa aaagctcaat actgagcagg
    15001 gtaaaggcga aaaatccact aaaaaatcga ctaaagccct gcaagagcag gaaaagcaga
    15061 tcaagaaaaa tgaaaaagtc gctaagaact tactgcaagc gattggcggt tttaccaaag
    15121 cgattggtgc attaggcaca atgattttgg ctggcgttgg acttgaccgc cttgtgcttg
    15181 atacagccaa agctaacaaa gaattggcta tgacatctaa gaacctggga atgaccagcc
    15241 aatcgttagc agcttggcga ggtgcagcag agctttcagg cggttctgca caggggctta
    15301 ccggttatct gaataacctt tctgccggtc ttacccgttt aactgtacaa ggtgatgcct
    15361 ctgttactca gtttttttct caactgggta tcaatctatt agacggctca caaaaagcca
    15421 aaaaacttga agacattatg ctggaattgg cagacaaatt cagctcaatg gatagagtaa
    15481 aagcctttgg cattgcccag caaatgggga ttgatgaagg tacgtttgaa atgctggcgc
    15541 agggcagaca aggtcttgaa gagcatttgc agaaaaccgc taaaatctac aaatccaatc
    15601 aacaggattt agaaaccgca cgcaaactga ccgcagcaac tgcctattta aaccagcaat
    15661 ttgaaggttt aaagctgatg attgccaatg ccgcaatgcc ggtattactc aaaattgccg
    15721 atgtaaccaa tgcgtttttt gaatatttgc agaaaaatga aaaccttgta aagggggtat
    15781 tttatggaat tgcgggggcg atctctattg ctctaatccc aatgcttatt agcggtgcaa
    15841 gtgctgcttt agcgtttatt gctccattcc tgccaatgat tttaattgtt gcggggttag
    15901 cagcagctat tggattgctc tacgatgatt atgaaaagtg ggcgaacgga ggtacttcgc
    15961 tatttaactg gggattattt aagaagtgga tttttgatgc agattttacc gttggcaatc
    16021 tgaaaaatgc gtttgccgat ttattaaccg gttataaatc ttgggaagac gcggttaatg
    16081 ctgctaagtc ttggctagaa cttaaaggct ttaccaaaga tgggaagatg tctatagata
    16141 gcttggcaac aggtttcaaa aatgtggcga aagatatata taactcgctt cttcctgcac
    16201 tagataaagt attaaatgtc atcaacctat tacttgatgg agaatttgtt gcagcgtggg
    16261 acgcagcaaa agagttaggg aaagacgcgt ttaatgccgc agtagatttt gttggtgata
    16321 atattgttgg tgatgcactg aaaggtgtcg gtggaataat agatgctggc gttactaata
    16381 tagaagaggc tgtcaatacg ggtaaaaaat attggaatgg cgagattaca ggctcagaag
    16441 ctcttaaaga agtaacaggg aattttgcca agaatgctga gaaagcagtc gatgaggttg
    16501 ttaattttgc agataacgcc attacaaggg tagaaaatgg atttgttcga tttgtgaata
    16561 tttggggcgg atttgaccca gatgaagttg gtcctaccgg taatgagaac aaactgatgg
    16621 atagcaagaa aggatttagt aaaggaaatt ttgtctttga ggggcaacaa tctgttaaag
    16681 gtcttacgca agaagaaaca ggggcattgg ctgctcaaat ggtgaagcgt gaaagtggtg
    16741 gaaacctcag agcggaaaat caatacggct atttaggatt atatcagttt ggtgcggccg
    16801 cattagttga tgcagggtta attgatgatg caaaatatag agcagcagta agaaaacacg
    16861 gtaaaggatt atctaatggt tctgatgctg atgttcacaa ggcattttta gcagattcaa
    16921 gcaactggac tattaaaggt ggtcgagaag ctttcttaag caataaagct attcaagata
    16981 atgcaatcgt agctcttatg aataagaatg ttggatattt aggctctact tatcaaggtt
    17041 ctgcggagca taaagctgga ttattgatgg cagctcactt aaaaggagct ggcggagcga
    17101 aagcttttgc aaattcaggt atagatagct cagatgggaa tggaacaaaa atatctgatt
    17161 actataatac agggcgaaag gcgattcagt tagctaaaca aggtaaatct attcagagca
    17221 agcctatcaa tttgaatacg ccagttggtg gctatgcagt agcaaatgca ctaaaagcta
    17281 atcagcatac ctttaccaca atgcaaaact tggctcaacc gcaaaatatt agcaacaacc
    17341 aaaaaacaga agttgcattt aatgggggaa ttcatataaa ctcaacagcg ggaacacttg
    17401 gcggaacagc tcaagatttt gttaatgggg tgcaaaaccg agtgaatgtg atgcaattta
    17461 atacgggatt gtcataatga aaagagcatt tatcctttta cctataattt tttcttccaa
    17521 tgcttttgct gatgagatgt tgttttcttg tttaacagaa aagaataata tcattagtct
    17581 catcaacaaa ggggatatgg ttgaatatca agttgctagc gctaaaacgg ataaaattac
    17641 attttcattt gaacagccaa tagggaatat caatctttct tcttctgttg agcaaggtgt
    17701 tactcaaaag gtgtatcaga tatcaaacaa tggatataga tacgatcttg ttgtgtggga
    17761 aaaagaaaat aataaactcc ctgttggggt agaggctaaa gatcctagcg gcaaaacagt
    17821 atcgactaat cgttgtaagg aatttataga tttcataaat agcgatactg ataataaagc
    17881 agaggaaaat acttcgagca atgaacctgt atccaatcag gtttatgaag aagggatgta
    17941 tcgtgtcggg cgagatattc cagcaggaga gtataaatta acaaccacaa ataaagagcg
    18001 tggttatttt acaatttatt ctagctctga gcgtgattct gtaattgctg ttgatacttt
    18061 taaaaattca acatatctca cggtgaaaga agggcagtat ttagaactta gaaatagtaa
    18121 tgcacagctt gtggaataat ccggttaatt tgaggtctat tatgtttaaa tttaaaaaac
    18181 aatctaatca aagtagcagt aaccaagaaa ataataaaat atctattgct gatgaattgg
    18241 acaaacttgt ttcgttaaaa gagaaaggta ttttatcaga actggaattt gaaaaagaaa
    18301 aagataagtt gttaaatgga gctaaatatc aagctcaaca acctacgttt attattcaaa
    18361 actcagcatc ttcatcttcg tcagcttctg caactgcggt ggttaagcgt tcgggttgtt
    18421 tatctagcat aatcaaggct cttttggctc tatttgtaat tttcttgata ttagatttta
    18481 tagcttggtc ttcagggaat aaaacagcgt cagaagtaaa agctaaacaa ctaatctctg
    18541 acaagcaatg caatcagcta ataaaagatc tgatttatca caaacacgct caagatattt
    18601 gtcttgttga ggcgaaactg ggagaattta attctaattt aaatggagat agttttgctg
    18661 aatactcaac tcgtaatggc tgtcctcaat tacctgaaaa tgtagcttat aaattacaag
    18721 aggaaacatc tttaaatttc ataaatgctc taaaatcttc accaagcaaa gttgagtttt
    18781 gtaaaaaaga actaccttat tttaaaaagc tagttcagaa atatgcttca taataaaatg
    18841 aaaaccataa aaaacgagta aaactactat agagtagagc ccattatgtt aaatgggctt
    18901 ttttattacc aaaaacaaac cccaagagcc gcaaactctt ggggtttttc atttaaccat
    18961 taatacggaa tgattaaata aattatgagc aattatagca aaacaaagtt aaaaatccac
    19021 ttaaaaaagg ggttagaaat ggaaacaaat gcaagtccta ttatgcgtgg tgcgatagct
    19081 atttcaatcg tacttatagc gttgggcgtt tttgccttat gtgtaacacc gttagcaaat
    19141 gtgttgattg agatgataaa atagctttac aaaccaaaat ttgagtagta ctatttcccc
    19201 ataggcgtcg aaacctaaac aaacagcggc aatccgcacc cgtcagacaa gcggtttttt
    19261 tgtgtctaaa ttttgcgatc tttttccagc cattagaaaa agattgtaca ggctcaatgt
    19321 cgagagggcg gagaatacaa tacccgaaag gggaataatc ccgaccgtct gtttgcggtt
    19381 ttcgaacctc tcggcaccct attgaatagg gaaaactaaa cttcgaaaaa acaaacagga
    19441 gcagacttat gtctaatcaa attactactc aaactctttc tttttacggt tcagatctta
    19501 ttactttaaa agttgaagat gttatttaca cggcagttcg cccgattgtt gatgcactag
    19561 gcttgtcttg gggttctcaa caaagaaaac ttgagaattc caaagaaaaa ttcagttgta
    19621 ctcatatgaa aacaacggga tcagacggca aaacttatga aatgctctgt atgccaatca
    19681 aaaaactcaa cggctggttg tttagcatta atcctgaaaa agtgcgggta gatttaaaag
    19741 aaaaagtgat ctgctaccaa gaagagtgtt ttgaggcact ctacaactac tggcacttag
    19801 gcaaagcgga gcgtttgcaa aattcagcgg aaaatcagac cgcacttccc gagccaaccc
    19861 tcacagcaga agagcaaaat atgctacaaa atgcggtaaa agccacgcac gaacgcacca
    19921 agctcagcta cggcgaaat
//
LOCUS       DANKBV010000036        19897 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.036, whole genome shotgun sequence.
ACCESSION   DANKBV010000036 DANKBV010000000
VERSION     DANKBV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19897
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.036"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            69..1241
                     /locus_tag="PWY43_001028"
     CDS             69..1241
                     /locus_tag="PWY43_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HDL2135919.1"
                     /translation="MNLTPFFIAFRYFRSKSADRFGRLVTNLASLGIVLGVMALVIVL
                     SVMNGLENMQKTNLLSTLPHAIITPQEGHFAKGEKLALPEFAKQSVVINRANVIIQSS
                     QGINAGQLIGVENATDDPLLVAENIPALLPSGEFKVVIGSRLANKLNVTVGDKLRLMI
                     TENSQYTPLGRVPVQRLFEISGVYQSNREASEFTLFANLADVGRLLRISEDQVQGVRL
                     YLQDPFQVTELSQYFDAERYQISDWREQKGEFFQAVKMEKNMMGLLISLIIVVAISNI
                     VTSLSLMVVDKQGEIAILQTQGLTKKQVMQIFVFQGAIVGVIGSIIGGLLGVLIAYNL
                     DQIIQQLNPSIHLPTLISGSQIAVIVGVSMLLSLLCTLYPAYRASKIEPAQALRYE"
     gene            1265..1948
                     /gene="lolD"
                     /locus_tag="PWY43_001029"
     CDS             1265..1948
                     /gene="lolD"
                     /locus_tag="PWY43_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDL2135920.1"
                     /translation="MTELLRCEHISKFYDEGEQKVQVLKEVSFSMNEGELVAIVGSSG
                     SGKSTLLHTLGGLDQPSTGEVWIRGQSLQQLSSDKLALLRNQNLGFVYQFHHLMADFT
                     ALENVMMPMLIGKQNKTEAVNRAEKMLQAVGLAHRMTHRPSALSGGERQRVAIARALV
                     NNPALVLADEPTGNLDQKTTESIFELIQQLNQEQNIAFLLVTHDLNLANKLSRSLVMR
                     DGVLSEGSL"
     gene            1945..3210
                     /gene="lolE"
                     /locus_tag="PWY43_001030"
     CDS             1945..3210
                     /gene="lolE"
                     /locus_tag="PWY43_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDL2135921.1"
                     /translation="MSTPFFISWRYQRGKQKNRLVSLIALFSNIGIALGVAVLIIGLS
                     AMNGFERELNNRVLSVVPHAELVSFQGNQPQPIFDSKKLEALVKKSQNVTASSPFVSF
                     TALIENGAQLKIAQVRGVDPKKQDQASQLSQFIPPEQWQAFNQQFQADEDGLILGAGI
                     AKALEVEAGDEVTLLIPQPTEDGKLAQPLRFNLNVTGILRLDGQLDHSYALIPLNKAQ
                     ELMEYQANEVSGLEISLANPFDVQQLEMPQLNGYTQPLYLNTWIDKFGYMYNDIQLIR
                     TVMYIAMVLVIGVACFNIISTLIMAVKDKQGDIAILRTLGANNGFIRRIFLWYGLISG
                     MKGALFGLILGVILSLNLTAIIKAIEAFFEVKLLSDGVYFVDFLPSELHWQDVVYVLA
                     ATIILSLFASLYPANRAAKLEPAKVLSGH"
     gene            complement(3285..5216)
                     /gene="thrS"
                     /locus_tag="PWY43_001031"
     CDS             complement(3285..5216)
                     /gene="thrS"
                     /locus_tag="PWY43_001031"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL2135922.1"
                     /translation="MPIITLPDGSQRSFDNPVSVMEVAQSIGAGLAKATIAGRVNGER
                     RDASDIISQDSALEIITAKDEDGLEIIRHSTAHLLGHAIKQLFPDVKMAIGPTIENGF
                     YYDIDLDRSLTQEDLDALEKRMLELAKTNYDVVKRPVSWQEARDTFEKRGEPYKMAIL
                     DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRI
                     YGTAWADKKQLAEYLQRLEEAAKRDHRRIGKALDLYHMQEEAPGMVFWHNDGWTIFRE
                     LETFVRTKLKEYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQSENREYAIKPMNC
                     PGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IESEVTSCIKMVYDIYSTFGFTNIAVKLSTRPENRIGDDAMWDRAEEGLANALRNNGL
                     EYEIQEGEGAFYGPKIEFALRDCLGREWQCGTVQLDFALPGRLEASYVAEDNGRRTPV
                     MIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQQVVKQLSDAG
                     IRVKADLRNEKVGFKVREHTLRRVPYMLVCGDKEIAEGKVSVRTRKGADLGTYLVSDL
                     VEILKNQIKARELKLLGEE"
     gene            complement(5383..6732)
                     /locus_tag="PWY43_001032"
     CDS             complement(5383..6732)
                     /locus_tag="PWY43_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2135923.1"
                     /translation="MNSTKFYSLYSGIFFLFFGYGLFLNSAGIKLAEMGITDIIIGLL
                     NAAFFIGAALSTIFAHRIVSSVGHIRSFSVFCAVFAIAALGHIMIDTLWIWGVLRIIL
                     GFCHYSLLLLVESWLSEKTNVDTRGKVLATYNIIFYLAFILGVSLLSLELSSRNIFIL
                     STILVVMSMIPIALTKMVQPDIPARERISIPKLFAISPLALATSFISGMLVNGFFTMA
                     SVFLLKFSFSLSEISLYLMISMIGGFISQMPMAILSNRIGRRNAILACSVIATLTALA
                     GLFAIFQENLTAWGQYIIAFFFGCSLFSLYALSIARANDELPNNMNTVEVSRGLLFCY
                     GVGALVAPPLIGFAMSLSAEYGFYALFGLFSLSLFICAFRTKSIPKAERAEMQYATPI
                     ATASPLEEIEIVEDALIPFDEEAVQEYQAQLEILEQDPQTENENIELDEEAAENSKV"
     gene            complement(6936..7502)
                     /locus_tag="PWY43_001033"
     CDS             complement(6936..7502)
                     /locus_tag="PWY43_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005711111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2135924.1"
                     /translation="MFTTQLIQTKRWLEANSITTFWLKTFIGATLLAIFAQISVPMYP
                     VPITGQTLALTVVGFAMGRKAATAAVLLYLFEGAIGLPVFAGGASGFHHFFGPTGGYL
                     LGYIPTAYFLGYYSDKGVLNSFWKSLFVALIASVITFAFGLAQLSFFVPEGTVLQAGL
                     YPFILGGVLKALLASVLVSPSYKFFSKL"
     gene            7583..8341
                     /locus_tag="PWY43_001034"
     CDS             7583..8341
                     /locus_tag="PWY43_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HDL2135925.1"
                     /translation="MVKPVYIYCDEGSSEIGVQSLLLAVKQHLQRPAYLINAKQIIQS
                     KFSDGCLLIMPGGADLPYCKKLNGEGNRRIRQFIEQGGAYLGICAGAYYGCAELNFVG
                     RDYSVNGNRELALFNGIAKGSLPQFTNGQLYDKNIGSKAIVEVTLAEGGKMPFYYHGG
                     CCFEPNSTTVIYNPLAYYPDGSVAMIEGELGRGYYLLSGVHFELQAVVYWQKIVNCCE
                     NLDIKTQEEAICSNFNVNYGSQIWQHLKQRLNQA"
     gene            complement(8332..9126)
                     /locus_tag="PWY43_001035"
     CDS             complement(8332..9126)
                     /locus_tag="PWY43_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2135926.1"
                     /translation="MRFFDTHTHLDYLAESLNLSIPELVENAEQHQVECILIVAVFAK
                     NFAKVTACAKQKPENLVYGLGLHPLYIQQHQQQDLILLENLLSQQDPQCTAIAEIGLE
                     KAVSEVSTPELWKKQCEFLEAQLSLAKKFNLPVNLHSRKSHDQLFVFLKKAQLTKTGV
                     VHGFAGSYEQAKRFVDLGYKIGVGGTITYERANKTREAIRKLPLESLLLETDSPDMPV
                     FGFQGQPNRPERLLVTFNSLCELRTESPNLIAETIWQNSERLFFYA"
     gene            complement(9187..10773)
                     /locus_tag="PWY43_001036"
     CDS             complement(9187..10773)
                     /locus_tag="PWY43_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDL2135927.1"
                     /translation="MLWFFFCVAVLVLGYFTYGKIIEKIFVINPKRSTPAYSMNDGVD
                     YMPMSKTKIWLIQLLNIAGTGPIFGPILGALYGPVAMLWIVIGCIFAGAVHDYFCGML
                     SIRNGGATMPNLAGKFLGRPVKLFINVMALILLLLVGVVFVASPAQLLSTITMDIAGA
                     TQSAMELGDVEAIKESVASGGVTVWGMDKATVISVWTLIIFAYYILATLLPVDKIIGR
                     IYPFFGALLLFMSVGMVYGLVSAHLSATDPIDFFRTVNADGQGLTWEKFTQNFQVKGD
                     VPIWPLLFLTISCGALSGFHATQTPLMARCAQNEKEGRFIFYGAMITEGVIALVWCAV
                     GLSFYENPQALQDAISAGSPSKVVYDSSLHFLGFIGGIFAVLGVVVLPITSGDTAFRA
                     ARLQLAEIFGLEQRTLVKRLYIAVPLFVLGFIVSKVDFSVLWRYFTWANQMTAMVMLW
                     TAAGYLYRYKKFHWVCSIPAWFITTVCATYLANNKIGFGLDYELSVYIGFAITVVCVI
                     CFFAFLKPSEARDEDAYVTN"
     gene            complement(10959..12812)
                     /gene="dxs"
                     /locus_tag="PWY43_001037"
     CDS             complement(10959..12812)
                     /gene="dxs"
                     /locus_tag="PWY43_001037"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL2135928.1"
                     /translation="MEKKYPLLSEINSPEDLRLLSKDQLQTVVDELRAYLLESVSQTS
                     GHLASGLGVVELTVALHYVYQTPFDQLIWDVGHQAYPHKILTGRRDQMHTIRQKDGIH
                     PFPWREESPYDVLSVGHSSTSISAGLGIAVAAEKENAGRKTVCVIGDGAITAGMAFEA
                     LNHAGALHTDMLVILNDNEMSISENVGALNNHLARIFSGSLYTSVRDGSKKILDKVPT
                     IKNFMKKSEEHMKGVIFSPESTLFEELGFNYIGPIDGHNIDELVKTLSNMRELKGPQF
                     LHIRTKKGKGYTPAENDPIGYHGVPKFDPTAAELPKSKAAPTYSNIFGDWLCEMAERD
                     PKIIGITPAMREGSGMVEFSKRFPEQYFDVAIAEQHAVTFGAGLAIAGYKPVVAIYSS
                     FLQRAYDQLIHDVAIQNLPVIFAIDRAGIVGADGQTHQGAFDLSFMRCIPNMTIMAPS
                     DENEMRQMLYTAYTMNTPSAIRYPRGNAKGVKLEPMQGLEVGKANIIREGKKVAILNF
                     GALLDEAKIVAEKHNYTLVDMRFIKPLDEALLQKVADSHELLVTLEENAIQGGAGSFV
                     NEYLQNIGKIKPLVMLGIPDFFVPQSTQAEAYADLGLDAQGIEAKIQKFDI"
     gene            complement(12857..13669)
                     /gene="suhB"
                     /locus_tag="PWY43_001038"
     CDS             complement(12857..13669)
                     /gene="suhB"
                     /locus_tag="PWY43_001038"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDL2135929.1"
                     /translation="MNPMLNIAIRAARKAGNIIAKGYEQSPQDTQVLQKNTNDYVTEI
                     DKAAEAAIIDVIRKSYPEHSIVTEESGVLNGSNENVQWVIDPLDGTTNFVKRLPHFAV
                     SIAVRENGRTTVGVVYDPIRNELFTAVRGEGAKLNEFRLRIDAERRDLKGTVIATGFP
                     FKVIRHRDAHLNMLEGLMNNGVADFRRTGSAALDLAYVAAGRVDGFFEIGLKPWDFAA
                     GELIAREAGAIVTNFVGGTDYLKTGNLVVGNGRVVKEILNSIQPTLTEELKA"
     gene            13850..14884
                     /locus_tag="PWY43_001039"
     CDS             13850..14884
                     /locus_tag="PWY43_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HDL2135930.1"
                     /translation="MQFKENNQPRFKHREERRSDERSPRFSDKGKMRGNEGKSDRPHK
                     LHFKRDEAYSRSEQIYPAEGKKASGEGSVQIWVKGGNSSVKEKKNGPLSPRAPEKIKK
                     NRLEEMKVYGENACISLFQQRPGSIVRLWATVEGAKKLGDLMSYLAQHKKAYHVIDRE
                     EMERVTGTEHHGDMCLLVKKTASFVLEGYLQVPKKQDCLVLLDGVNNAQNVGGIIRTC
                     AFYGVKGIITENVDSLNSSSAARVAEGGLEFVRALETKNKHIALVQLRQAGYQIVHLT
                     RHKQAPSLTKIKLAEKVVFVLSEIPASDVAYAEDTTIQLSFNNPLNSGLNVAVNAGIL
                     LNQWYQTHIL"
     gene            14894..15778
                     /locus_tag="PWY43_001040"
     CDS             14894..15778
                     /locus_tag="PWY43_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDL2135931.1"
                     /translation="MNQQKPFEVIAIVGTPRHDSAFETHLAVYNWLKDRDYQILVETE
                     VATQLQLKESYSLDQIGQRAELVIVIGGDGNMLGIARALAKYRVPLIGINRGNLGFLT
                     DIAPQTAFEQLYSCLVKNEYVIEERFLLEAFIERNGKIIAANNALNEIVVHPTQVARI
                     IEFEVYIDGKFAFSQRADGLIVATPTGSTAYSLSAGGPILTPNMNAMALVPMHPHTLS
                     SRPLVVDGDSQISLRFAKYNTPDLEVICDGQVNMHFTPEDRVLVRKSPDKLQLLHLKD
                     YNYFTVLGSKLGWASKLF"
     gene            15917..16894
                     /locus_tag="PWY43_001041"
     CDS             15917..16894
                     /locus_tag="PWY43_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1852 domain-containing protein"
                     /protein_id="HDL2135932.1"
                     /translation="MTTNFKCQVHTIEFNENYQPEQSTRLTTNFANLARGENRKQNLQ
                     NAIAMINNRFNDLANWDNPNADRYSVKLDIVSLDIDVEGKGEFFPAIEWLKTYVIDYK
                     TGKTIDGIIGNNFSSFVRDYDFSVLLLDHNKDKPQFSVPEGYGELHGKLFKHLLASPE
                     YTAKYKKPPVICLSVATTRTYTRTTNVHPILGVEYLQDSYSLTDAYFAKMGLKVRYFM
                     PPKSVAPYAFYFDESADLLNDYTNLELISTIATMEAFQKIYRPEIYNANSVAGQVYKG
                     SLTYQDYSVTKVEYDREERTQLAIKQGKYTEEHFIKRYQAILEAWATEE"
     gene            16982..18013
                     /locus_tag="PWY43_001042"
     CDS             16982..18013
                     /locus_tag="PWY43_001042"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2135933.1"
                     /translation="MSNILLPTSTAGSLPKPSWLAEPEKLWSPWKFEGEELLQAKRDA
                     LLVSLAEQIAAGIDIVSDGEQTRQHFVTTFIEHLEGVDFSKREIVRIRNRYDASVPSV
                     VGAVSRPKSVFVDDAKFLRAHTEKPIKWALPGPMTMIDTLYDGHYKSREKLAWEFAKI
                     LNEEAKELEAAGVDIIQFDEPAFNVFFDELNDWGVAALERAAEGLKCETAVHICYGYG
                     IKANTDWKQTLGNEWRQYEESFPKLQQSKIDIISLECQNSRVPMDLIELVRGKKVMLG
                     AIDVATNIIETPEQVADTLRKALQFVDADKLYPSTNCGMAPLSRQVAQGKLQALSQGA
                     AILRAEFAK"
     gene            18101..18322
                     /locus_tag="PWY43_001043"
     CDS             18101..18322
                     /locus_tag="PWY43_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135934.1"
                     /translation="MLNLFKKPIKKSSFEAWAKLMDDVAKVAILAIPVILYGEYSLLM
                     KFTHSITLFAVVYFLLATARLFRETAEEM"
     gene            18598..19860
                     /locus_tag="PWY43_001044"
     CDS             18598..19860
                     /locus_tag="PWY43_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL2135935.1"
                     /translation="MLKRDMNIADYDAVLWQAIQDEDRRQEEHIELIASENYASPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKALFGADYANVQPHSGSQAN
                     AAVYMALLNPGDTILGMSLAHGGHLTHGASVSFSGKIYKAEQYGITDEGLIDYEALRK
                     QAQEVKPKMIVAGFSAYSQVMDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPNPLP
                     HAHVVTTTTHKTLAGPRGGLILSASGDEEMYKKLQSAVFPAGQGGPLMHVIAAKAVCF
                     KEALEPEFKAYQQQVVKNAKAMVEVFKQRGFDVVSNGTENHLFLVSFVKQGLTGKAAD
                     AALGAANITVNKNSVPNDPQKPFVTSGIRVGTPSVTRRGFKEAEVTALAGWMCDVLDS
                     IGKDNHEQVIAETKAKVLDICKRLPVYA"
BASE COUNT         6005 a         4074 c         4263 g         5555 t
ORIGIN      
        1 agtggtaaac tccataggga cggaccgata tatccacccg tagtatctat tatttttaaa
       61 agaaacagat gaatttaacc ccttttttta ttgcttttcg ctattttaga tcgaaaagtg
      121 ctgaccgttt cgggcggtta gtcaccaatc ttgccagcct tggcattgtg ctgggcgtaa
      181 tggcattggt gattgtgctg tcggttatga acggtttgga aaatatgcag aaaaccaatc
      241 tgctatccac cttgccgcac gctatcatca cccctcaaga ggggcatttt gccaaaggtg
      301 aaaaactggc tctacctgaa tttgccaagc aaagtgtggt gattaaccgt gcgaatgtga
      361 ttatccaaag ttcacaaggc attaacgccg gtcagttaat cggcgtggaa aatgccacag
      421 acgatccgtt gttagtggcg gaaaatatcc ccgcattatt gcctagcggt gaatttaagg
      481 tagtaattgg ctctcgttta gccaataaat tgaacgttac ggtaggcgat aagctccgct
      541 taatgatcac tgaaaacagc caatatacgc cacttgggcg agtgccggta cagcgtttgt
      601 ttgaaatttc gggcgtttat caatctaatc gtgaagcgag cgaatttacc ctctttgcta
      661 atttagctga tgtcgggcgt ttgcttcgca tcagcgaaga tcaagtacaa ggcgtacgtt
      721 tatatttgca agatcctttc caagttaccg aacttagcca atatttcgat gcagaacgct
      781 accaaatcag tgattggcgt gagcaaaaag gcgaattttt ccaagcggtg aaaatggaaa
      841 aaaatatgat gggcttactc atcagcctga ttattgtggt tgcaatttcc aacattgtga
      901 cttcactcag tttgatggtg gtagacaaac agggcgaaat tgcaatttta caaacccaag
      961 gcttaaccaa aaaacaagtg atgcagattt ttgtgttcca aggggcgatt gtcggagtaa
     1021 ttggctcgat tattggtggt ttgctcggcg tattgatcgc ctacaattta gatcagatta
     1081 tccagcaatt aaacccgagc atacatctgc ctaccttaat ttcaggctcg caaattgcgg
     1141 ttattgttgg ggtgtcgatg cttctttcac tcctttgcac actttacccg gcataccgag
     1201 cctcaaaaat tgagccggca caggcattaa gatatgaata ataaataata agaacggaaa
     1261 taaaatgaca gaattacttc gctgtgaaca tatcagcaaa ttttacgatg agggcgaaca
     1321 gaaagttcag gtgctaaaag aggtgtcatt ttcgatgaac gaaggtgagc tggtggcgat
     1381 tgtcggtagt tcaggttccg gcaaaagcac cctattgcac acattaggcg ggctagatca
     1441 gccaagcacc ggtgaagtct ggattcgagg gcaatctctg caacagctca gttcagacaa
     1501 attagcgtta ttgcgtaacc aaaacttagg ctttgtgtac caatttcatc acttaatggc
     1561 agactttacc gcccttgaaa atgtgatgat gccgatgctg atcggcaaac agaacaaaac
     1621 tgaagctgtc aatcgtgccg agaaaatgct acaagcggtc ggattagctc atcgaatgac
     1681 acatcgccca tcagcacttt caggcggcga acgccaacgg gtggcgattg cccgagcgtt
     1741 ggttaataat ccggctctgg tacttgccga tgaaccgacc ggtaatttag accaaaaaac
     1801 cactgaaagc atttttgagc tgattcaaca gctcaaccaa gagcagaaca ttgcgttttt
     1861 attagtaacg catgatttaa atctagcgaa taaactttca cgtagtcttg taatgcgaga
     1921 tggcgtgtta agcgagggga gcttatgagt acgccgtttt tcattagctg gcgttaccaa
     1981 cggggcaaac agaaaaacag actggtttca ctaattgccc tcttctccaa catcgggatt
     2041 gcccttgggg ttgcggtatt aattatcggg ctaagtgcaa tgaacggctt tgagcgagag
     2101 ctgaataatc gtgtactttc ggttgtgccg cacgcagaat tagtttcgtt tcaaggcaat
     2161 cagcctcagc cgatttttga tagcaaaaaa ttagaggcat tggtaaaaaa atcgcaaaat
     2221 gtgaccgcta gttccccttt cgtgagcttt accgccttaa ttgaaaatgg ggctcagctt
     2281 aaaatcgccc aggtgcgagg ggttgatcct aaaaagcaag atcaggccag ccaattaagc
     2341 caatttattc cacccgagca atggcaagcg ttcaatcagc aatttcaagc cgatgaagat
     2401 ggcttgattt taggagccgg tattgccaaa gcgttagaag ttgaggcagg agacgaagtt
     2461 acgcttttaa ttcctcagcc aacagaagac ggcaaattag cccagccctt gcgttttaac
     2521 ctaaacgtaa ccggcatttt gcggctagat gggcagctgg atcacagtta tgccttaatt
     2581 ccactgaata aagctcaaga gctgatggaa taccaagcga atgaagtgtc aggacttgaa
     2641 atcagccttg ccaatccgtt tgatgtacaa cagcttgaaa tgccacaact gaacggctat
     2701 acccaaccgc tttatttgaa tacgtggatc gataaattcg gttatatgta taacgatatt
     2761 cagctgattc gcaccgtgat gtacattgca atggttctgg taattggcgt tgcctgtttt
     2821 aatattattt ccaccttgat tatggcggtg aaagacaaac agggcgatat tgcgatttta
     2881 cgaacacttg gtgcaaataa cggttttatc cgccgcattt tcttatggta tggcttaatt
     2941 tcaggtatga aaggggcgtt attcgggctg attttaggtg tgattttatc gctaaattta
     3001 accgctatta tcaaagcgat tgaagcattc tttgaggtga aattgctttc agacggagtt
     3061 tattttgttg atttcttacc aagtgaatta cattggcaag atgtcgtcta tgtattggcg
     3121 gccacgatta tcctaagcct ttttgccagc ttataccctg ccaaccgagc cgctaaatta
     3181 gaaccggcaa aagtgttaag tgggcattaa tcattttcct attctaataa aaaagcagct
     3241 acccctcgat tatgtcagaa aggtagctgc ttttatttat gttattactc ttcgcctaat
     3301 aatttcaact cacgagcctt aatttggttt tttagaattt caactaagtc tgaaacgagg
     3361 taagtaccta agtctgcacc tttacgggta cgcacagaca ctttaccttc ggcgatttct
     3421 ttatcaccgc aaaccaacat ataaggcaca cggcgtaagg tgtgctcacg cactttgaag
     3481 ccgacttttt cgttgcgtaa gtctgctttc acacgaatgc ctgcatcaga aagctgtttc
     3541 accacttgtt gcacataatc tgcttggctg tcggtaatgt tcattaccac tgcttgggtt
     3601 ggggcaagcc aagttgggaa gaaaccggca tattcttcgg tgataatccc gatgaaacgt
     3661 tcgatcgagc ctaaaatcgc acggtgaatc attaccggtg tgcggcgacc attatcttct
     3721 gctacataag aggcttctaa acgacccggt agagcgaagt caagttgcac cgtgccacat
     3781 tgccattcac gtcctaagca atcacgcaat gcaaactcaa ttttcggccc gtagaatgca
     3841 ccttcgcctt cttgaatttc atattcaagt ccgttgttgc gtaaggcgtt ggctaagcct
     3901 tcttctgctc tgtcccacat tgcatcatca ccgatacggt tttccggacg ggttgaaagt
     3961 tttactgcaa tgttagtaaa accgaaggtg ctgtaaatgt cgtacaccat tttgatacag
     4021 ctggttactt ctgactcgat ttgttcttca gtacagaaaa tatgtgcatc atcttgggta
     4081 aaaccacgca cacgcattaa gccgtgtaat gaaccggacg gctcgttacg gtgacacgaa
     4141 ccaaattccg ccatacggat cggtaaatca cggtaagatt tcaagccttg attaaagatt
     4201 tgaacgtgac ccgggcagtt catcggctta attgcatatt cacggttttc cgattgggta
     4261 gtgaacatta aatctgcgta gttttgccag tgtccggttt tttcccaaag cacacgatcc
     4321 atcataaacg gaccttttac ttcttggtaa tcgtactctt tcaatttggt acgcacaaaa
     4381 gtttcaagtt cacggaaaat cgtccagccg tcattgtgcc agaacaccat acccggtgct
     4441 tcttcttgca tatggtataa atctaacgct ttaccgatac ggcggtggtc acgttttgcc
     4501 gcttcttcca aacgttgtaa atattcggct aattgttttt tatcagccca agctgtaccg
     4561 taaatacgtt gtagcatttt atttttgctg tcgccacgcc agtaagcacc cgctactttt
     4621 tgtaatttga agtggtggca gaaacgcata tttggtacgt gtggaccacg gcacatatca
     4681 atatattctt cgtggtgata taacgcaggc gttgccgtac gttcaatatt ttcatctaaa
     4741 atcgccattt tgtatggctc accacgtttc tcaaaggtat ctcgagcttc ttgccagctt
     4801 accgggcgtt ttaccacgtc atagttggtt tttgccagct ctaacatacg cttttcaaga
     4861 gcgtctaaat cttcttgagt caaagagcgg tctaaatcga tatcgtagta gaaaccgttt
     4921 tcgatagtcg ggccgatagc cattttaacg tctgggaata attgcttgat ggcgtgacct
     4981 aacaagtgag cggttgagtg gcgaatgatc tctaaaccat cttcgtcttt ggcagtaatg
     5041 atttcaagtg cgctgtcttg gctgatgata tcagacgcat cacggcgttc accgtttaca
     5101 cgaccggcaa tggtggcttt cgctaaacct gccccgatag attgggcaac ttccattaca
     5161 gaaactgggt tgtcaaatga acgttgtgaa ccgtccggta aagtaataat tggcataata
     5221 aatccttaca gtggtaatcc atacgagaga ttacttgtgt tgtttgaatg aaaaaaccgc
     5281 ctacaagcgg tcttttttga ttaaaatttt gcaatttgct tattatttcc cttacaagtg
     5341 gggaataaaa gcactgaaat tctagcataa aacaaagaaa aattaaactt tgctgttttc
     5401 agcggcctct tcatcaagct caatattttc attttctgtt tgtggatctt gttcaagtat
     5461 ttcaagctga gcctggtact cttgtaccgc ttcttcatca aatggaataa gtgcgtcctc
     5521 aacaatttca atttcttcaa gtgggcttgc ggttgcaatt ggggtagcat attgcatctc
     5581 tgctcgttcc gctttcggaa ttgatttggt gcggaaagca caaataaata aactaagtga
     5641 gaacagtcca aaaagtgcat aaaagccata ttcagcggaa aggctcatcg caaaaccgat
     5701 aagcggtgga gcaactaacg cccccacacc atagcaaaac agtaaacccc tgcttacttc
     5761 aaccgtgttc atattattcg gcaattcatc gttggcacgg gcaatgctca aagcatataa
     5821 gctaaacaga ctacagccaa agaaaaaggc gataatatac tgtccccaag cggtcaaatt
     5881 ttcttggaaa attgcaaata atccggcaag tgcggttaaa gtggcaatca ctgaacaagc
     5941 taaaatggcg ttacgtctgc caatacgatt cgataatatc gccatcggca tttggctgat
     6001 aaaaccaccg atcatcgaga tcatcaaata aagcgagatt tcacttaatg aaaagctaaa
     6061 cttcagcaga aagactgacg ccattgtaaa aaagccgttt accaacattc cgctaataaa
     6121 gctggttgct aaggctaacg gtgaaatcgc aaatagttta ggaatactaa tgcgctctcg
     6181 agccggaata tcaggctgaa ccattttagt taaagcaatt gggatcatcg acatcacaac
     6241 caaaatggta gataagataa aaatgtttcg gctactaagt tctaaactta ataaacttac
     6301 gcctaaaata aaggcgagat agaagataat gttataagtg gcaagcactt tgcctctggt
     6361 atcgacattg gttttttcgc ttaaccaact ttcgactaaa agcagcaagc tataatgaca
     6421 gaatcccaaa ataattcgta atacgcccca gatccacaaa gtatcaatca taatatgacc
     6481 gagagcggcg atagcaaaaa cagcacagaa aacgctaaat gaacggatat gcccaacact
     6541 tgatacaatg cggtgagcaa aaatagtgct taatgccgcc ccaataaaga aagcagcgtt
     6601 aagtaaaccg atgatgatgt ccgttatccc catttctgcc agtttgatcc ctgctgaatt
     6661 tagaaataaa ccgtagccga aaaacagaaa aaagatacct gaataaaggg agtaaaattt
     6721 agtgctattc atcaatgctc cttcaaacat tctgccagta ttaaatcatt gtaccgattt
     6781 aatacctttt aggcaaaaaa gttattatta gcgttgagaa aataactttt ggctattttg
     6841 attaaaaaat caaatgaagg ggattttgaa atgtagtaaa aaatagagca atcagaattg
     6901 aaaaaggctt tgccgtaaca aagcctttag aaagattaga gtttcgagaa gaatttgtag
     6961 cttgggctaa ccaaaacact tgctaggagt gctttaagca caccacctaa aataaatgga
     7021 tataagcccg cttgtaatac tgtgccttct ggtacaaaga aagaaagttg agccaagccg
     7081 aaagcaaagg tgatcacact tgcaattaag gcgacaaata aacttttcca aaaagagttt
     7141 aatacgcctt tatcgctata atagcctaag aaataggcgg ttggaatata gcctaataag
     7201 tatccgcctg ttggaccaaa gaaatgatgg aaacctgaag caccgccggc aaagacaggt
     7261 aaaccaatcg ccccttcaaa aagataaagt aatactgcag cagttgcggc tttacgaccc
     7321 attgcaaaac caaccacagt taaagcaagt gtttgccctg taatgggtac agggtacatc
     7381 ggcacagaaa tttgtgcaaa aatagcaagc agtgttgctc caataaaagt tttcaaccaa
     7441 aatgtagtta tactattggc ttctagccaa cgtttagttt gaattaattg cgtagtgaac
     7501 ataatccttt cctttaatat ggttaaaatg ttctcaaccg attatatagg aagatgtctt
     7561 ttagggtaag agtgaattta aaatggtaaa accagtttat atttattgtg atgaaggtag
     7621 cagtgaaatt ggtgttcaat cactgttatt ggcagtaaaa cagcatttac agcgtccggc
     7681 ttatctcatc aatgccaaac aaattattca aagcaagttt tccgatggct gtttattaat
     7741 catgcccggt ggagccgatt taccttactg caaaaaatta aacggagaag gcaatcgtag
     7801 aatccgccaa tttattgaac aaggtggagc ttatctcggt atctgtgccg gagcttatta
     7861 tggctgtgct gaattgaatt ttgtagggcg tgactatagc gtaaatggta atcgagaact
     7921 agctttattt aatggcattg cgaaaggcag tctgccgcag tttaccaacg ggcaacttta
     7981 cgataaaaac attggtagca aagcgatagt tgaagtaaca cttgcagagg gtggaaaaat
     8041 gccgttttat tatcacggtg gttgctgttt tgaaccaaat tcaacaacag tgatttacaa
     8101 cccgttggct tattatcctg atggctcagt agcaatgata gagggagagc tagggcgagg
     8161 ttattatctg ttgtcgggtg tacattttga attacaagcg gtcgtttatt ggcaaaaaat
     8221 tgtaaattgc tgtgaaaatc ttgacataaa aacacaagaa gaagcaattt gctctaattt
     8281 taatgtaaat tatggctcgc aaatttggca acatctaaag caacgactaa atcaagcgta
     8341 aaaaaataac ctttcgctat tctgccaaat ggtttctgca atcagatttg gtgattctgt
     8401 tcttaattca catagactat tgaaagtcac taaaagccgt tccggtcggt tcggctggcc
     8461 ttgaaagcca aacaccggca tatcgggcga gtcggtttcc agtagtaaac tttcaagagg
     8521 tagtttgcgg atagcctcac gggttttgtt agcacgttca taggtaatag ttccccccac
     8581 accaatttta taaccgagat cgacaaaacg ttttgcctgt tcatagctgc cggcaaagcc
     8641 gtgaaccaca ccggttttgg ttagttgggc ttttttgagg aacacaaaaa gttgatcgtg
     8701 gcttttacgt gaatgaagat taacgggtaa attaaatttt ttcgcgaggg aaagttgtgc
     8761 ctctaaaaat tcacattgct ttttccataa ttcaggggta gaaacttcac ttaccgcttt
     8821 ttccaaaccg atttcggcaa tagcggtaca ctgtggatct tgttggctta gcaaattttc
     8881 caataagatt aaatcttgtt gttggtgctg ttgaatatac agtggatgca accctaatcc
     8941 atacaccaaa ttttccggtt tttgtttggc acaagcggtc acttttgcaa aattttttgc
     9001 aaatacggca acgataagaa tgcattcaac ctgatgctgt tcggcattct caaccaattc
     9061 cggaatgctt aaattcaggc tttctgcgag atagtcgagg tgagtatgag tgtcaaaaaa
     9121 tcgcataata aaaccaataa aaacggggac tagattgtcc ccatttttag cattttacgc
     9181 aagcaattag tttgttacat aagcatcttc atcacgagcc tcagatggct tgaggaaagc
     9241 aaagaagcaa ataacacaaa ctaccgtaat cgcaaaaccg atataaactg ataattcata
     9301 atctaaaccg aagccgattt tgttgttcgc aaggtaagtt gcacacacgg tagtgataaa
     9361 ccatgcagga atcgagcata cccagtggaa ttttttatag cggtataagt aacctgcggc
     9421 agtccataac ataaccattg cagtcatttg gtttgcccaa gtgaagtaac gccataatac
     9481 gctgaaatca acttttgaga caatgaaacc aagtacgaat aatggtacgg caatatacaa
     9541 acgttttact aaggtacgtt gttctaaacc aaagatttcc gctagttgta aacgagcggc
     9601 tcggaatgca gtgtcacctg aggtgattgg taatactaca acgcctaata cggcaaaaat
     9661 accaccgata aaccctaaga agtgtaacga gctgtcatat accactttag acggtgaacc
     9721 ggcagagatc gcatcttgta aggcttgtgg gttttcatag aatgaaagac ctactgcaca
     9781 ccatactaat gcaattaccc cttcagtaat cattgcaccg tagaaaataa aacgaccttc
     9841 tttctcattt tgcgcacagc gagccattaa tggggtttgg gttgcgtgga aaccggataa
     9901 tgctccgcaa gaaatagtta agaatagtaa tggccaaatt ggaacatcac ctttcacttg
     9961 gaagttttgg gtgaattttt cccacgttaa gccttgaccg tctgcattca ctgtacggaa
    10021 gaagtcgata ggatcagtcg cacttaagtg agcgctgact agaccatata ccatacctac
    10081 agacataaag agtaataatg ctccgaagaa aggataaatt cgaccgataa ttttatcaac
    10141 aggtagtaat gtagcaagaa tatagtaagc aaaaataatt aacgtccaca cagaaatcac
    10201 ggttgcttta tccatacccc aaacggttac gccacctgat gctacagatt ctttaattgc
    10261 ttcaacatca cctaattcca ttgcactttg ggttgcacct gcaatatcca tggtgatggt
    10321 agagagtaac tgagcagggc ttgcaacgaa aactacgcct actaataaaa gcaagatgag
    10381 cgccattacg ttgatgaata acttgactgg acgacccaag aatttccctg ccagatttgg
    10441 catcgttgcg ccaccgttgc ggatacttaa cataccacag aagtagtcgt gtactgcacc
    10501 ggcaaaaata caaccaatca caatccaaag cattgcaact ggtccgtata aagcaccaag
    10561 aatcgggccg aaaatagggc cggtgccggc aatatttaat aattgaatca gccaaatctt
    10621 ggttttagac attggcatat aatccacacc gtcattcata gagtaagccg gtgtcgatct
    10681 ttttgggtta atcacgaaga ttttttcaat gattttccca taagtgaaat agccaagcac
    10741 aagcacggct acacagaaaa agaaccacaa catagtagaa atcctataaa ttgagataaa
    10801 tgatcatttt tagcagaact gctaaaggcg tgccattgta agtgaggagt gaggataaag
    10861 taaaacgaat tgttagattt gtgtgaataa tttactgaaa atgtgaggtt catcaaaaaa
    10921 tccctttgtt ctgtatcaat caaagggact ttttaaagtt aaatatcgaa tttttgaatt
    10981 tttgcttcaa tgccttgggc atctaatcca agatcggcat aagcttcagc ttgagtagat
    11041 tggggaacga aaaagtcagg aataccaagc attacaagcg gtttgatttt gccgatattt
    11101 tgcaaatatt cattcacaaa actacctgca ccaccttgaa tagcattttc ttcaagcgta
    11161 accaacaact cgtgagaatc cgccactttt tgaagtaatg cttcatctaa tggtttaatg
    11221 aaacgcatat ctaccaaggt gtagttatgt ttttcagcca caattttggc ttcatcaagc
    11281 aaagcaccaa aattcaaaat cgcaactttt ttaccttcac gaataatgtt tgctttacct
    11341 acttcaagtc cttgcattgg ctcaagtttt actcctttag cattgcctcg agggtaacga
    11401 atggcacttg gcgtattcat cgtgtaagcg gtataaagca tttgtcgcat ttcattttca
    11461 tcgctcggtg ccataatggt catattggga atacaacgca taaaacttaa atcaaatgca
    11521 ccttggtggg tttgtccgtc tgcaccgaca attccggctc ggtcaatcgc aaaaatcacc
    11581 ggcaggtttt gaatagcaac atcgtgaatt aattggtcat aagctcgctg caagaagctg
    11641 gaataaattg cgactaccgg tttgtaacct gcgattgcta agcctgcccc gaaagtcact
    11701 gcgtgttgtt cggcaatcgc cacatcaaaa tattgttcag ggaaacgttt ggaaaattct
    11761 accattcccg agccttcacg cattgccggc gtaatgccga taattttcgg atcacgctct
    11821 gccatttcac atagccaatc gccaaaaata ttggaataag taggtgctgc ttttgatttt
    11881 ggtaactcgg ctgcggtagg gtcaaatttc ggaacgccat ggtagccaat cggatcattt
    11941 tccgcaggtg tgtagccttt gccttttttc gtgcgaatat gcagaaattg agggcctttc
    12001 agctcacgca tattgctcaa ggttttgact aactcgtcaa tattatgtcc atcaatcggg
    12061 ccgatataat taaagcctaa ctcttcaaac aaagtgcttt caggggagaa aatcacccct
    12121 ttcatatgct cttcgctttt tttcataaag tttttgatgg taggcacttt atctaaaatt
    12181 ttcttactgc cgtcacgcac tgaagtatag agcgaaccag aaaaaatgcg ggcaaggtgg
    12241 ttgtttaatg ccccgacatt ttcagagatt gacatttcat tatcatttaa gataaccaac
    12301 atatcggtat gcaatgcacc tgcgtggttt aatgcttcaa atgccattcc ggcagtgatt
    12361 gcaccatcac cgattacaca gacggtttta cggccggcat tttctttttc tgctgccacc
    12421 gcaatgccaa gtccggcact gatcgaagta gaagaatgcc caacgcttaa cacatcgtaa
    12481 gggctttctt cacgccaagg gaaggggtga ataccatctt tttggcggat agtgtgcatt
    12541 tgatctcggc gcccagtaag gattttatgt gggtaagctt gatgaccaac gtcccaaatt
    12601 aattggtcga aaggcgtttg ataaacataa tgcaaagcta ccgtaagctc tactacccct
    12661 aagccagagg ctaaatgtcc gctagtttga ctgactgatt ccaataaata ggctcgcagt
    12721 tcatcaacta cggtttgaag ctggtcttta gaaagtaaac gtaaatcttc cggtgagttg
    12781 atttcgctta acagaggata cttcttttcc ataatgtttt attacccaca accatcaaat
    12841 aatagatgat attaaattaa gctttgagtt cttcggtcaa agtaggctga atgctattta
    12901 aaatttcttt aaccacacgg ccgttaccaa caaccagatt acctgttttc aagtaatctg
    12961 ttccgccaac aaagttagtc acaattgcac cggcttcacg ggcaataagt tcaccggcgg
    13021 cgaaatccca cggttttaag ccgatttcaa agaaaccatc aacacgaccc gctgcaacat
    13081 aggcaagatc taaagcggca gaaccggtac ggcggaaatc agctactccg ttattcatta
    13141 agccttcaag catatttaaa tgtgcatcac gatgtctgat gactttgaaa ggaaaaccgg
    13201 ttgcaattac agtgcctttt aaatcacggc gttctgcatc aatgcgtaaa cggaactcgt
    13261 ttaatttagc cccttcgcca cgcaccgcag tgaaaagctc gttacggata ggatcgtaaa
    13321 ctacccctac tgttgtacgt ccgttttcac gcacggcaat cgacacagca aaatgaggta
    13381 agcgttttac gaaattggtt gtaccatcta atggatcgat cacccattgt acattttcat
    13441 tgctaccgtt taacacgccg gactcttcgg tcacgatcga gtgttcaggg taagatttac
    13501 ggatcacatc aataattgcc gcttccgctg ctttatcgat ttcagtcaca taatcattcg
    13561 tatttttttg taaaacttgt gtatcttgag gcgattgttc atagcctttg gcaataatat
    13621 tgcctgcttt tcgtgccgca cgaatagcaa tgtttaacat tgggttcata tttccaccag
    13681 attttaaaga acataataaa gcacgaatta taaagaatgt agcgaaataa agaaacaaca
    13741 agcggtctaa tctgtgaaaa attttgcaaa aaaatggctt tttctaaccg cttggagttt
    13801 ggattcgtta taatacctct cattcaagcc acccatttag gtactaaaaa tgcaatttaa
    13861 agaaaataac caacctcgtt ttaaacacag agaagaacgc cgttcagatg agcgttcccc
    13921 ccgtttttca gataaaggca aaatgcgagg aaatgaaggt aaaagtgaca gaccgcataa
    13981 gctacatttt aagcgtgacg aggcttattc acgctctgag cagatttatc cagcagaagg
    14041 caaaaaagcc agtggtgaag gtagtgtgca aatttgggta aaaggtggga atagctcagt
    14101 taaagagaaa aaaaacggcc cgctttcacc gagagcgcct gagaaaatta agaaaaatcg
    14161 cttggaagag atgaaagtct atggcgaaaa tgcctgtatc agtttgttcc aacaacgtcc
    14221 tggatctatt gtacgtttat gggcaacagt ggagggggca aaaaaattag gcgatttaat
    14281 gagctatttg gctcagcaca aaaaagccta tcacgttatt gaccgtgaag agatggagcg
    14341 agtcaccggt actgaacatc acggcgatat gtgcttattg gtgaaaaaaa cggcttcatt
    14401 tgtgctagag ggctatttac aagtgccgaa aaaacaagat tgcttggtac tgttagatgg
    14461 tgtaaacaat gctcagaatg tggggggaat catcagaacg tgtgcttttt atggtgtgaa
    14521 aggcattatt actgaaaatg tggatagctt aaactccagt tctgcagctc gtgtcgcaga
    14581 gggtggtttg gaatttgtgc gagcgttaga aactaaaaac aagcatattg ctttggtaca
    14641 actccgccaa gccggttatc aaattgtcca cctcacacgc cataagcaag ccccttcttt
    14701 aaccaaaatt aagctggcgg aaaaggtggt ttttgtatta agtgagattc ctgcaagtga
    14761 tgtggcttac gcagaagaca caacaataca gctttccttt aataatccgt taaatagtgg
    14821 tttaaatgtg gcggtaaacg caggtattct acttaatcaa tggtatcaaa cccacattct
    14881 ttaaggaatt gaaatgaatc agcaaaaacc gtttgaagtg attgcgattg ttggcacacc
    14941 tcgccacgac agtgcatttg aaacacatct tgccgtttat aattggttaa aagatcgtga
    15001 ttatcaaatt ttagtggaaa cagaggttgc cactcagtta cagcttaagg aaagctacag
    15061 tttagatcag attggtcagc gcgcagagtt ggtgattgtg ataggtggcg atggtaatat
    15121 gcttggcatc gcccgagctt tggctaaata ccgagtgcca ctgataggca ttaaccgtgg
    15181 caatctgggt tttttaaccg atatcgcacc tcaaacggct tttgaacagc tttatagttg
    15241 cttagtcaaa aatgagtatg tgattgaaga gcgattcctg ttagaggctt tcattgagcg
    15301 caacggtaaa attattgccg caaataatgc cttgaatgaa attgtggttc acccaaccca
    15361 agtggctcga attattgaat ttgaagttta tattgacgga aaattcgctt tttcacaacg
    15421 tgcagatggc ttgattgtgg caacgcctac aggctcaacc gcctattcac tttctgctgg
    15481 tggcccgatt ttaaccccga atatgaatgc aatggcatta gtgccgatgc accctcacac
    15541 tctttcgtct cgtcctttgg tggttgatgg cgatagtcaa atttcgcttc gttttgccaa
    15601 atataatacc cctgatttag aagtgatttg tgacgggcag gtgaatatgc actttacgcc
    15661 tgaagataga gtcttggtgc gtaaaagccc ggataaatta cagctgctcc atttaaaaga
    15721 ttataactac tttactgtat taggttctaa attaggctgg gcaagtaagt tgttttaaac
    15781 acaagcggtg ttttttctga ttaaatttgc aaatgcaata tgaattgatt tcatatacat
    15841 ttcaaaaata tatcattttt attcaataaa aatttgtggt tcaataagct atctgaaaaa
    15901 taaaggggac aataaaatga caacaaattt taaatgccaa gtgcatacta ttgaatttaa
    15961 tgaaaattac caaccggagc aaagcacccg tttaaccacc aactttgcca accttgcccg
    16021 tggcgaaaac cgcaaacaga acctgcaaaa tgcgattgcg atgatcaata accgttttaa
    16081 cgatttagcg aactgggata acccgaatgc ggatcgttat tcggtgaaat tggatattgt
    16141 ctcactggat attgatgtgg aaggcaaagg cgagtttttc cctgcgattg agtggctgaa
    16201 aacctatgtt atcgactaca aaaccggcaa aaccattgat ggcattatcg gtaataactt
    16261 ctcttctttt gtgcgtgatt acgatttcag cgtattgttg ttagatcaca ataaagacaa
    16321 accgcaattt agcgtgccgg aaggctatgg cgagttacac ggcaagctgt ttaagcattt
    16381 attggcatcg cctgaatata ccgcaaaata taaaaaacca ccggtgatct gtttaagcgt
    16441 ggcaaccacc cgtacctaca caagaaccac taacgttcac ccaattttag gtgtggaata
    16501 tctgcaagat agttattcat taactgatgc ctattttgcg aaaatgggct taaaagtacg
    16561 ttactttatg ccgccaaaat ctgttgcacc ctatgcgttc tactttgatg agagtgcaga
    16621 tttactcaat gattacacta acttagagct aatcagcacg attgccacaa tggaagcgtt
    16681 ccaaaaaatt taccgtccgg aaatttacaa tgccaattcg gtcgcagggc aggtctataa
    16741 aggcagcctg acttaccaag attattccgt caccaaagtg gaatatgacc gtgaagaacg
    16801 cacccagttg gcgatcaaac agggtaaata caccgaagag cattttatta aacgttacca
    16861 agcgatttta gaggcttggg cgacggagga ataaacgtag ggtgggcgta agcccaccat
    16921 aaaatgagat ataaccaatg gtgggcttac gcccacccta ctttttatag gaaaattcaa
    16981 aatgagcaac attcttctcc caacctctac cgccggcagc ctaccaaaac cttcgtggct
    17041 tgccgagccg gaaaaactgt ggtcaccttg gaaatttgaa ggcgaggaac tacttcaagc
    17101 caaacgtgat gccctccttg tttcccttgc cgaacaaatt gcggcaggga ttgatattgt
    17161 tagtgatggc gaacagaccc gccagcactt tgtgaccacc tttatcgagc atttggaagg
    17221 ggtagatttt agcaagcgtg aaattgtgcg cattcgtaac cgttacgatg ccagcgtgcc
    17281 gagcgtggtg ggggcagttt cccgtccgaa atcggtgttt gtggacgatg ccaaattctt
    17341 gcgtgcgcat accgaaaagc caattaaatg ggcattaccc ggcccgatga cgatgatcga
    17401 caccctctat gacggtcatt acaaaagccg tgaaaaactg gcgtgggaat ttgccaaaat
    17461 cctcaacgaa gaagccaaag agttggaagc ggcaggtgtc gatattattc aatttgacga
    17521 accggcgttt aacgtcttct tcgatgagct gaacgactgg ggcgtggcag cgttagagcg
    17581 tgcagcggaa ggcttaaaat gcgaaacggc ggtgcatatc tgctacggct acggtattaa
    17641 agccaatacc gactggaaac agaccctcgg caacgaatgg cgtcaatacg aagagagttt
    17701 cccgaaactg caacaatcaa aaatcgatat tatctcgtta gagtgccaaa actcccgtgt
    17761 gccaatggac ttaatcgaat tagtgcgtgg caaaaaagtg atgctggggg cgattgatgt
    17821 agcaactaac attatcgaaa caccggaaca agtggcagat acgttacgca aagcattaca
    17881 atttgttgat gcggataaac tctacccaag caccaactgc ggaatggcgc cactttcacg
    17941 ccaagtcgca caaggtaaat tacaggcact cagccaaggg gcagcgatat tgcgtgcgga
    18001 attcgcaaaa taattttgcg acctgcatcg aaaatatcaa ctttgctctt tgctcgtttc
    18061 cagattttct tccaaaataa caggaaatga cggaggcact atgctaaatc tatttaaaaa
    18121 gccaattaag aaaagctcct ttgaggcttg ggcaaaacta atggacgatg tggcaaaagt
    18181 tgcaatttta gcaattcctg ttattctcta tggagaatat tcactgctaa tgaaatttac
    18241 acactcaatt accttatttg cagtagttta ttttttgtta gcaacggcaa gattgtttcg
    18301 agaaacagca gaggagatgt aaatggacgg attgacaatc ggattatttc ttttgcttgc
    18361 attttcattt gctgcatacc aatgggcaaa gcatttaaaa gatcataacc attaattcaa
    18421 tgaataatac taagcggtca tatttggtaa attttttaca aaatgtgacc gcttgttttt
    18481 ctttcactcg aagttaattt ttatataaat aatactatct atgaaactaa aaaatgcgta
    18541 aaataacgtg aaatcaactt acgcaaacgt ttacttaagc ttattaggag tccaaaaatg
    18601 ttaaaacgtg atatgaatat cgctgattac gatgcagtat tatggcaagc cattcaagat
    18661 gaagatcgcc gtcaagaaga acatattgaa ttaattgcat ctgaaaacta tgcaagccca
    18721 cgtgtaatgc aggctcaagg ctcacaatta accaataaat atgcagaagg ttatccgggt
    18781 aaacgttact atgggggttg tgagtatgtg gatattgtag aacaacttgc gattgaccgt
    18841 gcgaaagcat tgttcggggc agattatgcc aacgttcaac cgcattcagg ctcacaagcg
    18901 aatgcagccg tatatatggc gttgcttaat ccgggtgaca ctattttagg tatgagttta
    18961 gcgcacggcg ggcatttaac ccacggtgct tcagttagct tctctggtaa aatttataaa
    19021 gcagaacaat acggcattac tgatgaaggc ttaattgatt atgaagcatt acgcaaacaa
    19081 gcccaagaag tgaaaccgaa aatgattgtt gctggtttct ctgcttattc tcaagttatg
    19141 gattgggcaa aaatgcgtga gattgccgat gaagttgggg catatttatt tgtggatatg
    19201 gctcacgttg caggcttaat tgcggcggga gtttatccaa atccgcttcc tcacgctcac
    19261 gtcgtgacga caacaaccca taaaacctta gccggccctc gaggtggttt gattttatct
    19321 gcatctggcg atgaagaaat gtataagaaa ctgcaatctg ccgtattccc tgctggtcaa
    19381 ggtggaccat tgatgcacgt tattgcggca aaagcggttt gttttaaaga agctttagag
    19441 ccggaattta aggcatatca gcaacaagtt gtaaaaaatg caaaagcaat ggttgaggta
    19501 tttaagcaac gtggttttga tgtggtttca aacggtacgg aaaaccattt attccttgtt
    19561 agctttgtaa aacaaggttt aaccggcaaa gcggcagatg ctgcattagg agctgcgaac
    19621 attaccgtga ataaaaactc ggtgccgaac gaccctcaaa aaccattcgt gacttcaggt
    19681 attcgtgtgg gtacgccttc tgtgacccgc cgtggtttta aagaagcaga ggtgacagca
    19741 ttagccggct ggatgtgcga tgtgctagat agcattggta aggataatca cgaacaggtg
    19801 attgcagaga ctaaagcgaa agttttagac atttgtaaac gtttaccagt gtacgcataa
    19861 tctcaacttt acactttttc aagcggtaat attttta
//
LOCUS       DANKBV010000037        19255 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.037, whole genome shotgun sequence.
ACCESSION   DANKBV010000037 DANKBV010000000
VERSION     DANKBV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19255
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.037"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..9464)
                     /locus_tag="PWY43_001045"
     CDS             complement(<1..9464)
                     /locus_tag="PWY43_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDL2135936.1"
                     /translation="MNKHCFRTIFSKTLQRIVVVSELAKSAGKSESESMTSGAILQKI
                     CKIRPLAFSLFCALGFITLSDNAMAETLIIQADSTAPKNQQPIILQTANGLPQINIQT
                     PNDKGLSHNKYSQFDVAEKGAILNNSRTNIATQQAGMVQGNPYLARGEAKVILNEVNS
                     SKPSVMKGYVEVAGKKAEVIIANPSGLHCDGCGIINSDRATFTTGKPQINNGNLDSFV
                     VEKGKVTVAGKGLDNSRVDYTDILAREAKINAGVWSKKETKVITGKNTVKRSNSDNNL
                     QIIHTNQPLAGEEKPQVAIDVGELGGMYAGKIHLIGTEQGVGVRNAGHIGASADTLTI
                     DSQGRIVNSGTFNAQRQVQLNAQHGIDNQGKIENKQGNIQLNSKADIQNSGSIVARGG
                     NIQKKAETKIQQSGETVAKGNITYTARQIHADKNSLIAAGVDVTDTAQGEVRKLEAQS
                     VAGKTIQLTAPEKTTVQGKNIASGKINVAAAEIDANQSQNNAHTVEYSAKSGDIQANS
                     AQFSANQIKLTTPKWLSTEGSQLTAEVIEAQQANLNARGSVWKQTGEQDFRLKAESIH
                     TDSARFSVGGHFEIDGNRLTNNQGLLSSGKRLGLNLNDAVDSVDGKLVAKGELSIRAP
                     LLNNQQGLILSGEKQAITVNTLKNQSGVITSQTEQTLDITSELQNQGGRISASGVAIN
                     AQSLDNREQGEISSVKELALTVSQQVDNRHGVIQSVGNLTLNSTLLDNQGGVLKSATD
                     ITLNVPTVLNNRISENGSLIEAGGSLTMNSFEVENQGTLARQPQPIQGIIASQFVLNS
                     TKQLNNEKGGIYIGSSARFTLSDSLNNRNGEILSWGDLAIFGGKSLTMTNTEGKVSAN
                     NGLEITAKALSGDGDVQAGDIRLNLQENFDITKDINANRSMIIQTEGDILNRKKLAAN
                     DRLQLTANQITNAQNARISSADTRLTATSTVYNEGLINSRSDNDNAQTVIKAQEIHNI
                     GTGNIYGDKVALGAERILNQDKDGTSATIAARQSLGLAAKVIENNTLVYEANKKSGSL
                     IYSGGEIAIGRQLNEQDTVIGNAEQLHNRSSIIEAEGRIQLNADKVANVNSHYRSELE
                     TTDRQTVNEHFILPRDKHNRGGSDVYDNGHLPTINVNQLRKGYYRKTWSYWQKYDEAN
                     LPVITDISQLNATTVLAKPYAQSCANDESVDCTLLPAGNYKKDNPVWTWFNLTPPAED
                     APEMTPEIIQAMNEEDLANQHLSEAEQEKLQKEQGQGIAPVVPARPIEPVRGSTETEA
                     QYQKRLQTYQAELKAYEAAEAAYQRYVLLKPLIDWEAKYGANIQALDEAIAKHNKANL
                     SQEYPHFWNLWIKERMVKENITKASLPAQILAGGDLTYQSDEFINDKSWVIAGKALNP
                     IGRSRIANLDDADAVRQDWELGRRQFSFTEYHGGTRRRHSRYDENDGPLARLKEVHKD
                     MNIFVELENTSPTAYTDYVDAKAANAVKPEGNQVERGNLPDAPNPPTMAIERIPSPNA
                     AAFEVRSMTVDTRLPNQGLYRINPQADSHVLIETDPDFTDKKRWLSSDYMFNALRHDH
                     NNVHKRLGDGFYEQRLIREQINRLTGRQFLGDYTDFDSQYKALMDAGITFAQKFNLRL
                     GITLSPEQVASLTSDIVWFETQTVRLPSGETVSVLVPKVYALARKGDIDGKGSLISAD
                     ALTINSKEIVNQGTIAGRRLAQFNAERLENSGTLSGGVLAAKVSGDLENIGGKIEADK
                     AILLDIAGNFNHTSTTRTTQIDLDGFKRAETNLDRKALLHVKDENGLLQVQAGNITSA
                     GADIINDGQGQTYLSAKNQLNLTALQVGFDEKMGGGNHYRNEALNDVVISRVQGKGDV
                     VVSGKNLYSEGADLDAKARLALLAENDMVLGSATRSRDYEEYHKTKSGGALAKKTKTT
                     YDNISETEHKGTELTGEDILVKAGHDIQGESLLAVSRSGDIDIIGGNNVTLSSATNQL
                     SEQHIRETKRSGFLNGGEIGFTIGSQKTRQENSLEGAIQSTARNTLGSEGGNIRIQAG
                     NKASVSNVDVLISNEKTAVISGKNGVRVESGKDVINSTDNYEFSQSGLSIALSTPVTD
                     AVQSARQSVQKAKATQNEKLKGVYAVKAAEDAVIAAQNAQKVAETLGNLGNALTENHA
                     AAENPAVKISVSLGTQKQQRESRSQSVTHSKSTLNGGNIALISGAGKVALEGVDTRVK
                     DTLLLDGKLGIESKGVADTYQNNTKNKNHSASVGVFIGLDRDSFGIGFEAGVSGGKGK
                     ENIETETWQNNQLQAGKIVTNSANGKLMLDATNVKANHWEGEVQDFEARSRQDISKYK
                     SEYTEGSVHGTLAVGTTGVNGHVAYNGAKLNTAQVENQTAIDIGKGGMDVKVKKNAHF
                     EGAVMTSQAEKENNRFQAGTLTTGDIENHSELKTRSVAISGGTSGVNPMSALSLLGNK
                     NESERSTTKAAIGENIAITLTEDLNAETTLNHLNRDTQNANQKVTKHDISEVKETQEL
                     VKGIGEIAEKAYQIYTHSEREKVTEAKLELGKAQAQKASKEEIGKLEANLDRLQKEFD
                     KNYGTGSKTKRAVEAVTAALQGLATKDVGQAAVGLASPYLNAEIKKYTEGDTQANLIA
                     HALLGAVEAAAAGNNVLAGAAAGAGSEAAADFIVKTLYKGKSTQDLTEAEKQNVALLS
                     QLASGLASGLIGDSTQSAAVGADIGKRAVENNYLSNWQEQKRSQELKSCHGDARCENQ
                     VRLYWGLVDIGQDVSFVSGAVAGVPENLAESVEGLISMFSSPIETIVAMKELIAREDT
                     LSYIANMTKQEYLAKLDKMQVYYEQAGAVGSFNAGKEFGNMMTDVVTALTGAGALVKT
                     GVGTTAKIVTKSGKELAIPKTRSSASLGTCSFHGDMEVKTDKGYRQISSIKVGDKVLA
                     KNERTGITTYQKVQAHYSNPYDYTVYVEVADSKGKYHTIVSNKIHPFFTQVLSGRVPI
                     SSEGHSYNGEIAKAQWVDAQHLQKGYRLLAESGEWQTVTKVEIKQERLKAYNMTVEKD
                     HTYFIRGTNADNEGVWVHNDCWHALPNDAKQVKNIDGYKAYQFTDPKDGKVVTVVQKD
                     LNRFETPNHQAGVDPHFNRLSQQIDAETGRYLSTDNPVDGLYNRAYLRSETLQKIYAN
                     YEKLPNGNYRDLDGNIIKGPIDIGHMYGWEHRRLSLA"
     gene            complement(9517..11292)
                     /locus_tag="PWY43_001046"
     CDS             complement(9517..11292)
                     /locus_tag="PWY43_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL2135937.1"
                     /translation="MKTPQNLILSTVLLSASFVAEANSSSLVQQARIDATQQQIDAAQ
                     QHRQQQYQQNQAEKFQPKADVRLETQITDTLTLSNHELPCYPIHQITLIDYSPDNSLT
                     TSQFQWAFEKAAKSLHLTLPHCFGGEGLAVLMKQIQNEIIGKGYVTTRVVTAEQDLRG
                     GKLVLTVIPGKIRNTLVVDSGKVPRFTKLHALTGFTFAEGDILNVREIEQSLENLKRV
                     PTAEANIEILPSSSEKAEVGDSDLKISYSQAFPFRLNLGLDDAGSKSTGKLQTSATLS
                     IDNIFSANDLFYTSFTHSMKQKGDDNGRRASKNLTLYYSVPFGYWQLAFSHTHSRYHQ
                     EVFGAFDKSYLYAGESDTDKLTLSYLLYRDAKRKTTLSGSLWSRQSQNYIDGAEIGVN
                     KRRMAGWEAGFSHKEYFPTATLELSANFKRGTAARGALSAPEEDYGEGTSRPKIITAS
                     ASLTKPFFWGKQPWQWQSSLNAQWNKTPLVPQDRFSIGGRYTIRGFDGELTLSGERGW
                     NWRNELSWQFNPSHAFYWAVDGGRVSGWATENQLGHHLMGTAIGFRGGVKGFSYDLFA
                     GRPMRKPEGFKTSDTVAGFNIGYNF"
     gene            11448..12533
                     /gene="queA"
                     /locus_tag="PWY43_001047"
     CDS             11448..12533
                     /gene="queA"
                     /locus_tag="PWY43_001047"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL2135938.1"
                     /translation="MLVSDFHFNLPDELIARYPTAERSASRLLHLNGETGEFADQQFV
                     DLYDHINEGDLLIFNNTRVIPARLYGRKPSGGKVEALVERVLDEHCCLAHVKASKAPK
                     EGAELIFGEDKLGEGKGFTATMVARHDTLFELVFNEDQPLFELLQQAGHMPLPPYIDR
                     PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNAMLEKLKAKGVDTAFVTLHVGAGTFQ
                     PVRVETIEDHKMHAEYAEVSQEVVDKILATKAKGKRVIAIGTTSVRSVESAAQAAEKD
                     GKLIAPFFSDTAIFLYPGKTFKVIDALVTNFHLPESTLIMLVSAFAGYRNTMNAYAHA
                     VETKYRFFSYGDAMFINKNPNALSDVP"
     gene            complement(12560..13098)
                     /locus_tag="PWY43_001048"
                     /pseudo
     CDS             complement(12560..13098)
                     /locus_tag="PWY43_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13111..15402)
                     /locus_tag="PWY43_001049"
     CDS             complement(13111..15402)
                     /locus_tag="PWY43_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL2135939.1"
                     /translation="MTLDRFCIALILFHLPILWLPKAFLFVGIVIGTLVLILSVYLRS
                     KLTAVLGLLVCIGYIQIIQVAKNAEKTTASKTPVQFEISKILKQTDYQTAIATLNTGE
                     KIYLNWQSEQPLLLNQFYQAELALRPISARSNIGNFDRQRWYFANHINMTATVRKVET
                     LAKTDYSLRTNWLNKVKAETDALPTQGFLLALAFGERAWLKTEHWEQFQQTATAHLIA
                     ISGLHIGLAMLFGVYFAKGVQWLCLKSELTALQAVGFSLYFARIVSFITAFGYSYLAG
                     FAGFAVPTARALLAIAFVLLCQVLRRHYTPWQYWGRIVALLILIDPLSLLSDSFWLSI
                     LAVASLILWYQYFPLSRFLNDEYRKKITKFNRLWLSLLHLQIGIWLIFSPVQLYFFEG
                     ISAFALLANLIIVPLYSFLLVPLILFTLLTDNLFSTWLLADYLAQFSLWLLDPFSNAW
                     FALSHWQQWQLLSLNLLVLVLLYCKLYRLSYKKWLSAVAVSLLFNLSFYLPKLFNQTQ
                     TEWIMFDVGQGLAMAFIYQQNKAVIYDTGSSWQANDGSRNSMAKMEILPYLKRNGIEV
                     EAIFLSHDDNDHSGGVVELLQAYPKARLISSSKVTYHNINPEPCIAGTSWQFGEWQLL
                     AIYPSHIAERAKNQDSCVILAKNDRLKILLTGDSGVEQERQFAHQLGKIDFLQVGHHG
                     SKSSTGETLLSITQPAFAVISAGRWNPWKLPNKQIIEKLNRRHINVLNTVETGMIRVK
                     FYPSSIEIEQARGAFSPWHKQFY"
     gene            complement(15544..16758)
                     /gene="sstT"
                     /locus_tag="PWY43_001050"
     CDS             complement(15544..16758)
                     /gene="sstT"
                     /locus_tag="PWY43_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL2135940.1"
                     /translation="MSQSLSSKILGGNLVLRIAIGLVLGVLLAFINKEWATSIGVLGQ
                     FFVKSLRAIAPILVFVLVLAAIANKEVGSDSKLKPILVLYVLGTFFAALTAVVLSFML
                     PTTLELTASPDGLTPPQGIGEILKTVIFNLVDNPLVALSNANFIGILAWSIGLGIAFR
                     HGSASSKAFLNDLANAVSFVVKVVIAFAPIGVFGLVAETVAINGIDAFEDYARLLVVL
                     VGAMLFVALVLNPLLVYWKIRRNPYPLTFTCLRESGVTAFFTRSSAANIPVNMNLAKR
                     LGVRDEIASVAIPLGASINMAGAAITVTVLTLAAAYTQGIQPDFATALLLSVVASICA
                     CGASGVAGGSLLLIPLACSLFNIPNDIAAQVIGVGFVIGVIQDSVETALNSSTDVLFT
                     AAVSYAEDQKSA"
     gene            17013..17375
                     /locus_tag="PWY43_001051"
     CDS             17013..17375
                     /locus_tag="PWY43_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2135941.1"
                     /translation="MKPTNKADFQHVINAAGYSMKGLKAAYINEAAFRQEIWCATILF
                     PLGLILGETNIEKALLVGTVLLVLVTELLNSAVESVVDRIGADFHELSGRAKDIGSAA
                     VFMAMVLLAITWILILFC"
     gene            17412..19055
                     /locus_tag="PWY43_001052"
     CDS             17412..19055
                     /locus_tag="PWY43_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDL2135942.1"
                     /translation="MRLKKTLLSVALLSATSAMAYETGKTYRFTVLHTNDIHGHFWQS
                     DKGEYGLAAQKTLVDNIRKEVAEKGGSVLMLNAGDANTGVPESDMQNARPDIEGMNAI
                     GYDAMVLGNHEFDYPLQILSMQEKWAKFPFISANVINKKTDKPIVKPYVMLERGGLKI
                     AVIGLTTEDTAKLGNPDVTENAFFKNPIETAKNTLAEINQKEKPDVRIALTHMGYYFD
                     GKHGTNAPGDVTMARTLEKGAFDLIIGGHTHDTVCIDEKGQFKAKYTPGEECKPDFQN
                     GTWIVQAGEWGKFVGRADFEFKDGKTTLVKYELIPVNLKKTIKKEDGTKEYRLYQPEI
                     KADEATYTLLKKYQDEGDKKLNIEVGRVKGLLDGKREHIRFQQTNLGRLIAQSQMERV
                     KADIGIMNSGGIRTSLEEGVITYKHILTVQPFGNMIATVDLKGQELLDTLTTIALKEV
                     DTGAYPQFAGISMVVDRTAKTISDVKIGGKPFDKEKTYKVSLPDYVAAGGDGYPLLKK
                     HPSYVNTGFIDAEMLKKFFEENKIIEADKYDPKNQIIFK"
BASE COUNT         5135 a         4410 c         3841 g         5869 t
ORIGIN      
        1 gctaatgata atcggcgatg ttcccagcca tacatatgac ctatatctat aggacctttg
       61 attatatttc catctaaatc tcgataatta ccatttggca atttttcata attagcataa
      121 attttctgta acgtttccga gcgtaaataa gctcggttat atagcccatc taccggatta
      181 tctgtcgata aatagcgtcc cgtttcggca tcaatttgct gagacaaacg gttaaaatgt
      241 ggatctaccc cggcttgatg gtttggcgtt tcaaaacgat tgagatcttt ttgaactact
      301 gtcaccactt tcccgtcttt cgggtctgtg aactgataag ctttatagcc atcaatattt
      361 tttacctgtt tggcatcatt cggtaaagcg tgccaacaat cattatgcac ccatacccct
      421 tcattatcgg catttgtacc tctaataaag taagtatgat ctttctctac cgtcatattg
      481 taggctttta atctttcctg tttgatttct accttagtga cagtctgcca ctcaccactt
      541 tcagcaagta agcgatagcc tttttgcaaa tgttgagcat caacccactg agcttttgct
      601 atctctccgt tgtaagaatg cccctccgaa gaaatcggca ctctgcccga taatacttgc
      661 gtaaagaacg ggtggatttt gtttgacaca atggtgtggt attttccttt gctgtctgcg
      721 acttctacat acaccgtata atcgtatgga ttactgtaat gagcttggac tttctgatag
      781 gtggttatac cggtgcgttc atttttcgcc agcaccttat cgcccacttt aatgctggaa
      841 atctgtcgat aacctttatc agttttgact tccatatcac cgtggaatga gcaagtgcct
      901 aaactagcgc tcgatctggt cttaggaatc gcaagttctt tacctgattt ggtgactatt
      961 ttcgccgttg tccctacacc tgttttaaca agagcaccag caccggttaa tgctgtcacc
     1021 acatcagtca tcatattgcc aaactctttt ccggcattaa aagaacctac agcccctgcc
     1081 tgttcatagt aaacctgcat tttatcgagt ttagctaaat actcctgttt agtcatatta
     1141 gcaatatagg agagcgtatc ttctcttgcg atcaattctt tcatcgccac aatagtttct
     1201 attgggcttg agaacatgga gatcaatcct tcaacgctct cagcgagatt ctcaggaaca
     1261 ccagcaacgg cgcctgatac aaaactaaca tcttgcccaa tatcaactaa tccccaatat
     1321 aatcgaactt ggttttcaca gcgggcatca ccatggcagg attttaattc ttgtgagcgt
     1381 ttctgctcct gccagttcga gaggtagtta ttctccactg cccttttccc aatatccgcc
     1441 ccaaccgctg ccgattgtgt actatcccct atcaagcccg aagccagtcc gcttgcaagc
     1501 tggcttagca acgccacatt ctgtttctcg gcttcggtta agtcttgcgt ggatttgccc
     1561 ttgtagaggg ttttaactat aaagtccgct gccgcttcgc tccctgcgcc tgccgcagcc
     1621 cctgccagta cattgttccc tgccgcagcc gcttccaccg cacctagcaa tgcgtgagca
     1681 atcaagttag cttgggtatc gccttcggtg tactttttga tttctgcatt taagtatggt
     1741 gaggctaaac ctactgccgc ttgtcccacg tctttcgtcg ctaaaccttg caaggctgcc
     1801 gtcaccgctt ccaccgctcg tttggtttta ctgcccgtgc cgtagttttt atcaaactct
     1861 ttttgcaagc ggtctaaatt ggcttctaat ttaccaatct cttccttgct cgctttttga
     1921 gcttgtgctt tgcccaattc cagcttcgct tccgtcactt tttcacgctc actatgcgtg
     1981 taaatctgat aagccttttc cgcaatctcg cctatgcctt tcaccagctc ctgtgtttct
     2041 ttcacctcac tgatatcgtg tttcgtcact ttttgattgg cgttttgcgt atctcggttg
     2101 agatgattaa gcgtagtttc cgcatttagg tcttcggtga gggtgatagc gatattttct
     2161 ccaatcgctg cctttgttgt gcttcgctcg ctttcgtttt tattgcccag taagctcagt
     2221 gccgacatcg ggttcacccc acttgtccct ccgctaatcg cgacacttcg ggttttcaat
     2281 tcactgtggt tttcaatatc gccggtggtc agcgtgccgg cttggaagcg gttgttttct
     2341 ttctccgctt ggctagtcat taccgctccc tcaaaatggg cgttcttttt cactttaacg
     2401 tccattccac ccttgcctat gtcgattgca gtttggtttt ccacctgagc agtgttcaac
     2461 ttcgctccat tatatgccac atgaccattt actcctgttg tccctaccgc caatgtgcca
     2521 tgtacgctgc cttctgtgta ttcgctttta tatttgctta tatcttgtcg acttcttgcc
     2581 tcaaaatctt gtacctcacc ttcccaatga tttgctttga cattggttgc atccagcatg
     2641 agtttaccat ttgcagaatt tgtaacaatt ttacccgctt gcagttggtt gttctgccac
     2701 gtttcagttt cgatattctc cttgccttta cctccactca ctcctgcctc aaaaccaata
     2761 ccaaaactat ctctatctaa accgataaat acgcccacac tcgcactgtg attcttattc
     2821 ttagtgttgt tttgataagt atccgcaaca cctttggatt caatacccag cttaccgtca
     2881 agcagcaggg tgtctttcac tcgggtatcc accccttcca gcgcaacttt gcccgctccc
     2941 gaaatcaagg caatattgcc gccgttcagg gtgcttttgc tgtgggtcac gctttgacta
     3001 cggctttctc tttgctgttt ttgcgtgccg agactgactg aaatcttcac cgccggattt
     3061 tctgccgcag catggttttc tgtgagtgca ttgccaagat tgcccaacgt ttctgccact
     3121 ttctgagcat tttgtgctgc aatcacggca tcctccgccg cttttaccgc atatacccct
     3181 ttgagtttct cgttttgtgt tgccttcgct ttttgcaccg attgccgtgc cgattgcacc
     3241 gcatccgtta ccggtgtact caacgcaatg cttaagcccg actggctaaa ttcataatta
     3301 tcggtgctgt taataacatc tttccctgat tcaacccgta ccccgttttt gcctgaaatc
     3361 acggcggttt tttcgttgga aatcagcaca tcgacattgc tcacgctcgc tttattgccg
     3421 gcttgaatcc gaatattgcc cccttcactg cccaatgtat tgcgtgccgt gctttggatt
     3481 gccccctcta agctgttctc ttggcgtgtt ttttggctac cgatagtaaa gccgatttca
     3541 ccaccgttca agaaaccgct gcgttttgtc tcacgaatat gctgttctga aagttggttt
     3601 gttgccgaac tgagcgttac attattaccg ccgataatat caatatcgcc actgcgtgag
     3661 acggcaagaa gactttcacc ttgaatatcg tgaccggctt tcactaaaat gtcttcaccg
     3721 gttagttcag tgcctttgtg ttcggtttca gaaatattgt cataagtcgt ttttgttttc
     3781 ttcgctagcg caccaccact ttttgttttg tgatactctt cataatcgcg agaacgtgtt
     3841 gcgctaccca acaccatatc attttcagcc agtaacgcta aacgcgcttt ggcatccaaa
     3901 tccgctcctt cactgtaaag gtttttccct gaaaccacca catcgccttt gccttgcacc
     3961 cgtgaaatga ccacatcatt caaggcttca ttacgatagt gattgccacc gcccattttt
     4021 tcatcaaaac caacctgtaa agcggtcaaa ttcaactgat tttttgcaga taagtaggtc
     4081 tgcccctgtc cgtcattgat gatgtctgcg cctgctgagg tgatgttgcc tgcctgtact
     4141 tgcagcagtc cattttcatc tttaacgtgc agtagggctt tgcggtcaag gttggtttca
     4201 gcgcgtttga aaccgtctag gtcaatctgt gtggttcgag tggtggaggt atggttaaaa
     4261 ttgcccgcaa tatccagcaa aatcgcttta tccgcttcaa tcttgccgcc gatattctct
     4321 agatcaccgc tcactttggc agcaagcacg ccaccactga gtgtgccact attctctaac
     4381 cgttcggcat taaactgtgc taaacggcgg cctgctatcg tgccttggtt tacaatctct
     4441 ttactgttga tggtaagcgc atcggctgaa atcaggctgc ctttgccgtc aatatcacct
     4501 ttgcgtgcaa gggcatacac tttcggcact aataccgata ccgtttcacc gcttggcaag
     4561 cgaaccgttt gggtttcaaa ccaaacaata tcgctggtca agcttgccac ttgctccggc
     4621 gataaggtaa tgcctaaccg aagattgaat ttctgcgcaa aggtgatgcc ggcatccatc
     4681 agggctttat attggctatc aaaatccgta tagtcgccca aaaattgtct gccggttaag
     4741 cggttgattt gctctcgaat taagcgctgt tcataaaaac catcgcccaa acgtttatgc
     4801 acattgttat ggtcgtggcg tagggcattg aacatataat cactgcttaa ccagcgtttt
     4861 ttatcggtga aatccggatc ggtttcgatc agaacgtggc tatccgcttg agggttaatg
     4921 cgataaagcc cttggtttgg caaacgggta tcgaccgtca ttgagcgaac ctcaaatgcg
     4981 gctgcattcg ggcttggtat gcgttcaatc gccattgttg gcggattagg ggcatcgggt
     5041 agattacccc gttcaacctg attgccttcc ggcttcacgg catttgctgc tttagcgtcc
     5101 acataatctg tataagcggt cggtgaagta ttttccagct cgacaaaaat attcatatcc
     5161 ttatggactt cttttaaacg agcgagagga ccatcatttt catcgtagcg ggagtggcga
     5221 cgtcttgtgc ccccgtggta ctcagtaaaa gagaattgtc gccttcccag ttcccaatct
     5281 tggcgaacag catctgcatc atcaaggttg gcaattctac tgcgaccaat cgggtttaac
     5341 gctttgcctg caataaccca acttttatcg ttgataaatt catcgctttg ataagtgaga
     5401 tcgccacctg ccaagatctg tgccggtaag ctggctttgg tgatattctc ttttaccata
     5461 cgctctttaa tccacaaatt ccagaaatgc gggtattctt ggcttaaatt cgctttattg
     5521 tgctttgcaa tcgcttcatc taatgcttga atattggcac catattttgc ttcccaatca
     5581 attaacggtt ttaatagcac ataacgctga taagccgctt ctgccgcttc ataggctttc
     5641 agttccgctt gatatgtttg tagtcgtttt tggtattggg cttccgtttc cgtagagcct
     5701 ctaacaggtt caatcggtct tgccggaacc accggtgcaa tgccttgccc ttgttccttc
     5761 tgtaattttt cctgctctgc ctcacttaaa tgctgattcg ctaaatcttc ttcattcatt
     5821 gcttgaataa tttcaggcgt catttccggt gcatcttcag ctggtggtgt taaattaaac
     5881 cacgtccata ctgggttatc ttttttataa ttacctgcag gtaatagagt acaatctaca
     5941 ctttcatcat tcgcacagct ttgagcatag ggttttgcca atacggtcgt ggcattcagc
     6001 tggctaatat cggtaattac cggcaagttt gcttcatcat acttttgcca atagctccac
     6061 gttttgcgat agtagccttt gcggagttga ttcacattaa ttgtcggcaa atgcccgtta
     6121 tcatatacat cacttccacc tcgattatgc ttatctcttg gcaaaataaa atgttcgttc
     6181 acagtttggc gatcagtggt ctccaattcc gaacgataat gactatttac attcgctacc
     6241 ttatccgcat tcaactgaat acgcccttca gcttcaataa tactgctgcg gttatgtagc
     6301 tgctctgcgt tgccaataac ggtatcttgc tcatttaatt gacgtccaat cgctatctct
     6361 cctccgctat atattaatga accgcttttc ttattcgctt cataaactag ggtattattt
     6421 tcaattactt tagccgctaa accgagagat tgacgagcag caatagtggc agaggtcccg
     6481 tctttatctt gatttagtat cctctccgca cctaacgcca ccttatcgcc ataaatattc
     6541 cctgtgccga tattgtggat ttcttgagct ttaatcacag tttgagcgtt atcgttgtcc
     6601 gagcggctat tgattaagcc ttcgttatag acggttgaag ttgcagtcaa acgggtatct
     6661 gccgagctta ttcgggcatt ttgtgcattg gttatttggt ttgccgtgag ctgcaagcgg
     6721 tcatttgcgg ctaatttttt gcgattgaga atatcgccct cagtctgaat aatcatcgac
     6781 ctattcgcat tgatatcttt ggtaatatca aagttctctt gcaaattcag gcgaatatcg
     6841 cctgcttgta catcaccatc accggataac gctttggcag tgatctccaa accattattt
     6901 gcagaaactt ttccctcggt attcgtcatt gtgagtgatt ttccgccgaa aatagccaga
     6961 tcgccccaac ttaaaatttc cccattacgg ttatttaagc tgtcgctgag ggtaaatcgt
     7021 gctgatgagc cgatataaat accgcctttt tcattattga gctgttttgt gctgttcagt
     7081 acaaactgcg atgcaataat cccctgtatt ggctgaggtt ggcgtgccaa ggtaccttga
     7141 ttttccacct cgaaactgtt catcgtaagt gagccacctg cttcaataag tgagccattt
     7201 tccgagatcc gattatttaa gacagtcggt acattgagcg tgatatctgt ggccgatttc
     7261 agtaccccac cttggttatc caaaagcgtg ctatttaggg tgagatttcc cacactttga
     7321 ataacaccgt gccgattatc tacttgttgg gaaaccgtga gcgctaactc ttttactgaa
     7381 ctaatttctc cctgctcacg gttatctaag gattgagcgt taatcgcaac accgctggcg
     7441 gaaattcgcc cgccttgatt ttgcagttcg ctagttatat caagggtttg ctccgtttga
     7501 gaggtgatca cccccgattg attttttaac gtattgaccg taatcgcctg tttttcgcca
     7561 ctcaaaatca gaccttgctg attattcagc aatggagcac gaatagataa ctcgcctttt
     7621 gcaaccaatt tgccatcgac tgaatccaca gcatcattta aatttagccc taaacgcttg
     7681 ccactactta atagcccttg gttatttgtt aaacgattac cgtcaatctc aaaatgtcca
     7741 cccactgaaa agcgagccga gtcagtatgg atactctctg cttttaggcg aaaatcctgc
     7801 tcaccagtct gcttccaaac cgagccacga gcatttaaat tcgcttgttg ggcctcaatc
     7861 acctctgcgg ttagctggct tccttcagtg gaaagccatt ttggggtggt gagcttgatc
     7921 tgatttgcac taaactgcgc actattcgcc tgaatatcgc ccgattttgc actgtattca
     7981 acggtatggg cattattttg gctctgattg gcatcaatct cagctgccgc cacgttaatt
     8041 ttccccgaag cgatattttt tccctgcaca gtggtttttt ctggggcagt gagttgaata
     8101 gttttccctg ccacagattg agcttccagc ttccgtactt caccttgtgc agtgtcggtt
     8161 acgtccaccc cagcggcaat cagtgagttt ttgtcggcgt gaatttggcg ggcggtgtag
     8221 gtgatattgc cttttgcgac cgtttcgcca ctttgctgga ttttagtttc ggcttttttc
     8281 tgaatattgc cgcccctcgc cacgatggaa ccgctatttt gaatatcggc tttcgagtta
     8341 agctgaatat tgccctgttt attttcaatt ttgccttggt tgtcaatccc gtgttgggca
     8401 ttgagttgca cttgtctttg agcgttgaac gtgccgctgt ttacaatccg cccttgactg
     8461 tcaatggtga gcgtatcggc acttgcaccg atatgcccag cgttacgcac gcccacgccc
     8521 tgttccgtac caatcaggtg gattttgccg gcatacattc cgcccagctc acccacatca
     8581 atggccacct gcggtttttc ttcccctgca agcggttgat ttgtatgaat aatttgcaaa
     8641 ttgttgtccg aatttgaccg cttgacggtg tttttgccgg taatgacttt agtctctttt
     8701 ttcgaccaga caccggcgtt gattttcgct tctcgggcga ggatatcggt gtaatccact
     8761 cggctgttgt ccagcccttt gccggcaacc gttaccttgc ctttttccac cacaaagcta
     8821 tccagattgc cgttgttaat ctgtggttta cctgtggtaa aagttgctcg gtctgaattg
     8881 ataatgccgc agccgtcaca gtgcaagccg ctcgggttgg cgataatcac ttccgctttt
     8941 ttgccagcca cttccacata acctttcatt accgaaggct tgctggaatt gacttcattt
     9001 aaaatcacct tcgcttcgcc acgggcaaga tacgggttgc cctgcaccat tccggcctgt
     9061 tgggtggcga tgttggtgcg gctgttgttg agaattgccc ccttctctgc tacgtcaaat
     9121 tggctatatt tgttgtgcga taatccttta tcgttcgggg tttgaatatt gatttgtggc
     9181 agcccgttgg cggtttgcag aataatcggc tgctgatttt tcggggcggt gctatcggct
     9241 tgaataatca aggtttccgc catagcgtta tctgataagg taatgaatcc caacgcacag
     9301 aacaagctga acgcaagcgg tcgaattttg caaatttttt gtaaaattgc accgcttgtc
     9361 atactttcac tttctgattt accggctgat tttgctaatt cagataccac cacgatacgt
     9421 tgtaaggtct tgctgaaaat agtgcgaaaa caatgcttat tcataataat tccttaatag
     9481 cacaatatag ttaaatataa tacgctcaat tatttattaa aaattgtagc caatattaaa
     9541 accggcaacg gtatctgagg ttttaaaccc ttccggtttt cgcatcggtc taccggcaaa
     9601 caggtcataa ctaaaccctt taactccacc ccgaaaccca atggctgttc ccattaaatg
     9661 atgtccgagc tgattttccg ttgcccaacc ggacactcga ccaccatcta ccgcccaata
     9721 aaaagcgtgg ctcggattaa attgccagct caactcatta cgccagttcc agccccgctc
     9781 accggaaaga gtcagttcac cgtcaaatcc acgtatggtg taacgcccgc caatcgaaaa
     9841 acggtcttgc ggaaccagag gggttttatt ccattgggca tttaggctac tttgccactg
     9901 ccatggctgc ttgccccaaa aaaacggttt cgtcaggctt gcacttgccg tgatgatttt
     9961 agggcgagaa gtcccttcgc cataatcttc ctcgggagca gataatgccc ctcgtgctgc
    10021 cgtacctcgt ttgaaattag ccgaaagctc aagcgtagcg gtcgggaaat attctttatg
    10081 cgaaaaaccc gcttcccagc ccgccattcg gcgtttgttg acaccgattt ctgctccatc
    10141 aatatagttt tgcgactggc gtgaccaaag tgaaccggac aaggtcgttt tgcgtttggc
    10201 atcacgataa agcaaataag agagcgttaa tttatcggta tcactttcac cggcgtagag
    10261 ataactctta tcaaaggcac caaatacttc ttggtgatag cgactgtgag tgtgtgaaaa
    10321 cgctaactgc caatagccaa acggcacgga atagtaaagc gtgagatttt tactggcacg
    10381 cctgccgtta tcatcgcctt tttgtttcat cgaatgggta aaagaggtat agaataggtc
    10441 atttgccgaa aaaatattat ctatagacag agtggcggag gtttgtaatt tacctgtgga
    10501 tttcgagccg gcatcgtcca gcccgagatt taagcgaaat ggaaacgctt gcgaatagct
    10561 gatttttaaa tcactgtctc ccacctccgc tttttcgctt gagctgggta aaatctcaat
    10621 atttgcctcc gccgtcggca cccgttttag gttttccaac gattgctcaa tctctctcac
    10681 attcagaata tcgccttcgg caaaagtaaa accggttaag gcgtgtagct tggtaaaacg
    10741 cggtactttg ccgctatcta ccactaaagt attgcgtatt ttgcccggaa tcacagttaa
    10801 aactaatttt ccaccacgta aatcttgctc cgcagtaacc actcgtgtag tgacataacc
    10861 tttgccgata atttcatttt ggatttgctt catcaacaca gctaaccctt cacccccaaa
    10921 acaatgcggt aatgtcaaat gaagtgattt tgctgctttt tcaaacgccc attggaattg
    10981 gctggtagtc agagaattgt caggagaata gtcaatgagg gtgatttgat gaatggggta
    11041 acaaggcaat tcgtgattgg aaagagtaag tgtatcagtg atttgtgtct cgagccgtac
    11101 atcagctttc ggctggaatt tttctgcttg attttgttgg tattgctgtt ggcgatgttg
    11161 ctgagcagca tcaatttgtt gttgagttgc atcaatgcgt gcttgttgta caaggcttga
    11221 agaatttgct tcagcaacaa aagaagcact taacaatacc gtgcttaaaa tcagattttg
    11281 tggggttttc ataagatcaa ataagtaaga cattcaacgt ttttagtcta taaaaattta
    11341 tgttgttttg caaactccgt tttatggagc tttatacata tctgacgact ttggtttttt
    11401 atgctattat ctccgctatt tttatactat agagatatag aataaccgtg ttagtttcag
    11461 attttcattt caacttaccc gatgagctga ttgctcgcta cccgactgcc gaacgctctg
    11521 ccagccgttt attacactta aatggcgaaa caggcgaatt tgccgatcaa caatttgtgg
    11581 atttatacga ccatatcaac gaaggtgatc tgttgatctt caataacact cgagtgatcc
    11641 cggctcgcct ttacggtcga aaaccgagtg gcggtaaagt ggaagcatta gtagaacgtg
    11701 tgttagacga acactgttgc ttggctcacg ttaaagcctc aaaagcaccg aaagaagggg
    11761 ctgaattgat ctttggggaa gataagctag gtgaaggaaa aggctttaca gcaacaatgg
    11821 tagctcgcca cgatacccta tttgaattgg tgtttaacga agatcaaccg ttattcgaat
    11881 tgctacagca agctggtcat atgccacttc caccttatat tgaccgcccc gatgaagatg
    11941 ctgaccaaga gcgttatcaa accgtttata gcaaagtgtt aggtgcagtt gccgccccta
    12001 ccgccggttt gcattttgac aatgcaatgc ttgaaaaact aaaagcaaaa ggggtggata
    12061 ctgccttcgt aacattgcac gttggtgccg gcactttcca gccggtacgg gtagaaacca
    12121 ttgaagatca taaaatgcac gccgaatatg ccgaagtgag ccaagaagta gtcgataaaa
    12181 ttctcgcaac gaaagccaaa ggaaaaagag tgattgcaat tggtacgaca tcagtacgct
    12241 cggttgaaag tgccgcccaa gcggctgaaa aagatgggaa attgattgct ccgttctttt
    12301 ctgacaccgc tattttcctt tacccgggaa aaacctttaa agtcattgat gcgttagtca
    12361 ccaatttcca tttaccggaa tccacgctaa ttatgttagt ctcagcattt gccggctata
    12421 gaaatacgat gaatgcctac gcccacgcag tagaaactaa ataccgtttt ttcagctatg
    12481 gcgatgcgat gtttatcaat aaaaacccaa atgcgttgag tgatgttcct taatttatga
    12541 gggttataag ggaataacat tatgaacaaa aacaatcttc caaccatttt aagggattgt
    12601 tttcaatata ttcaataatt tggaagtacg atgtttcatt acgaataatg tgttcataat
    12661 aattcctttg ccaaatttca gaaatatcgg tattttgacg aacccattta gttacgccaa
    12721 ttttaaaacc acgcacaata gagccgattg ttcgtgatgt tccccgtaag ggcgaaacat
    12781 ttttcgccct tatacatggg gcaaatgatg attttcccct acagtttcga cgatttcaat
    12841 aatgccatgt aaatgattag gcataatcac aaagctatgc aagcttacat ttggaaaatg
    12901 atcgggtata tcttcccaac attgacgtac aatattccct atcttattaa gcctcatttc
    12961 atcatcaaca atctcaccga gaaaatggtg tttattttta caacaaatag taatgaaata
    13021 gaatccttgt gatcggtaat cataatgctt tagacgaatt gaacgcctcc tatgaatctc
    13081 cggctgatat ttctgcattt ttcttattcc ttaataaaac tgcttatgcc aaggggaaaa
    13141 tgcccccctt gcctgctcaa tttcaataga acttgggtaa aattttactc ttatcattcc
    13201 cgtttctacg gtatttaata cattaatatg tcttctgttt aatttctcaa taatctgttt
    13261 attcggtaat ttccacgggt tccatcttcc agcagaaatc actgcaaatg ctggctgggt
    13321 aatcgataat aaagtttcgc cagtgctgct tttgctaccg tgatgtccaa cctgtagaaa
    13381 atcaattttg ccgagctgat gggcaaattg gcgttcttgt tccacgccag aatcccctgt
    13441 cagtaatatc ttcaagcggt cgtttttggc taaaataacg caagagtctt ggtttttagc
    13501 tcgttctgca atgtgtgacg ggtaaattgc caataattgc cattcgccaa attgccacga
    13561 tgttccggca atacaaggct cgggatttat attgtgatac gtcactttgc ttgaactaat
    13621 taaccttgct tttgggtatg cttgcaataa ctccactacc ccacctgaat gatcattatc
    13681 atcgtgactt agaaaaatcg cctctacctc aatgccattt cgctttaaat acggcaagat
    13741 ttccattttt gccattgaat tgcgactacc atcatttgct tgccaactgg agccggtatc
    13801 gtaaattacc gctttatttt gttgataaat gaacgccatt gccagccctt gcccaacatc
    13861 aaacataatc cattctgttt gggtttgatt aaagagctta gggagataaa aactcagatt
    13921 gaacaatagg gataccgcta ctgcacttaa ccacttttta taagatagcc gatataattt
    13981 gcaatacagc aatactaaaa ccaataaatt cagtgaaagt aactgccact gttgccaatg
    14041 gcttaatgca aaccacgcat tagaaaatgg atctaacagc cataggctaa attgagcaag
    14101 atagtccgcc aacagccacg ttgaaaacag attgtcggtt aagagcgtaa ataaaataag
    14161 cggtacaagc aaaaagctat acaacggcac aataatcagg ttggcaagca gtgcaaaagc
    14221 agagatgccc tcaaaaaagt agagttgaac cggtgagaaa atcagccaaa taccgatttg
    14281 taagtgcaaa agcgataacc acaagcggtt aaattttgtg atttttttac gatattcatc
    14341 gtttaaaaat cggctaagag gaaaatattg ataccataaa attaaacttg ctaccgctaa
    14401 aattgagagc caaaaactat cagaaagcag gctcaatggg tcaattaaaa tcagtaacgc
    14461 cactattctc ccccaatatt gccaaggggt atagtgtcgc cgcagaactt ggcaaagcaa
    14521 gacaaacgca atcgccagca acgctctggc ggtcggcacg gcaaaaccgg caaaaccggc
    14581 taaatagctg taaccaaatg cggtgatgaa gctaactatt cttgcaaaat aaagtgaaaa
    14641 tccgaccgct tgtaatgcgg taagctcgct tttcaagcaa agccattgca cgcctttggc
    14701 aaagtaaaca ccaaacaaca ttgccagccc gatatgcaag cctgaaatgg caattagatg
    14761 ggcagtagcg gtttgttgga attgctccca gtgttctgtt ttcagccacg ccctttcacc
    14821 aaaagccaga gccaataaaa agccttgcgt aggcaaagcg tccgtttcag ctttcacttt
    14881 attcagccaa tttgttcgca agctataatc agttttggct aatgtttcca ccttgcgaac
    14941 cgttgctgtc atattgatat gattggcaaa ataccaacgc tgtcggtcaa aattaccgat
    15001 attgctacga gctgaaatag gacgaagagc cagttcggct tgatagaatt gattaagtaa
    15061 caacggctgt tccgattgcc agttcaagta gatcttctcg cctgtattta gtgtcgcaat
    15121 cgctgtttga taatcggttt gttttaagat tttactgatt tcaaattgaa caggcgtttt
    15181 actagcagtc gttttttccg cattttttgc aacttgaatg atctgaatat agccaataca
    15241 aaccaataag ccgagtacag ccgttaattt tgagcgtaga taaacactca atatcagcac
    15301 taaggttcct attactatac ccacaaatag aaacgcttta ggcaaccata aaataggcag
    15361 atgaaataga atcagggcaa tacaaaaacg gtctaaagtc ataattcggc ttacttttta
    15421 aagctgagta tgccgtttat gcttaatatt ttcagaataa attttcagat tttcgacaca
    15481 gttcgcaaaa atacttgaaa aaataaaggc agacttttta gcctgcctta taaagaagag
    15541 atattatgct gatttttgat cttcagcgta acttactgct gcagtgaata atacgtctgt
    15601 tgatgaattt aatgcggttt caaccgagtc ttgaatcaca ccaatcacaa aacccacccc
    15661 aattacttga gcagcaatat cgtttggaat attaaataaa ctacaggcta atggaatcaa
    15721 taataatgaa ccgcctgcca cacctgatgc gccacaagca cagattgatg caactacgct
    15781 taatagtagt gctgttgcga aatcaggttg aataccttgt gtataagctg ccgctagggt
    15841 taatacggtt acagtaatcg ctgcacctgc catattaata cttgcaccta atggaatagc
    15901 tacagaagcg atctcatcac gaacgcccaa acgcttggct aaattcatat tcaccggaat
    15961 gttggctgct gaactgcggg taaagaatgc cgttacacca ctctctcgta aacaggtaaa
    16021 tgtaagtggg tatgggttgc ggcgaatttt ccaatacact aataatggat ttagtactaa
    16081 tgccacaaat aacattgcgc caactaagac tactaataaa cgtgcgtaat cttcaaacgc
    16141 atcaatacca ttgatggcaa cagtttcagc tactaaacca aataccccaa taggcgcaaa
    16201 tgcaatcaca actttcacaa caaacgaaac tgcgttagct aaatcattta agaatgcttt
    16261 tgaacttgcc gaaccatggc ggaatgcaat ccctaaaccg atagaccacg caagaatacc
    16321 gataaagttt gcattactta atgcgactaa tggattatca actaagttaa aaataacggt
    16381 ttttagaatt tcaccaatgc cttgtggcgg ggttaaaccg tctgggcttg cagtaagctc
    16441 taatgtagtt gggagcataa agcttaaaac aacagccgtt aatgccgcaa aaaatgtacc
    16501 taatacataa agcaccagaa tcggctttaa tttgctatct gaaccaactt ctttatttgc
    16561 aatcgcagcc aaaactaata caaatactaa aataggcgca atggcacgta gtgatttcac
    16621 aaaaaattga cctaatacac caatgctggt agcccattct ttattaataa atgccagcaa
    16681 tacccctagc actaaaccaa tggcgatacg taatactaaa ttaccgccaa gaatttttga
    16741 tgataatgat tgactcataa cccttcactt ctgtaagaca acaaaaaata aggataaata
    16801 aaatttatcc gcctgaaaaa ataggtttat aagattagcg gaaaataaac aaatatggaa
    16861 taaacaagcg gtttattttt cagatttttt tacaaaaaag tgaagttttt tacattccat
    16921 ggctatcata ttattttttc cttaggttct ttgaacatca ccattctttc agtacaatag
    16981 ttctctaaac tcttttattt attttattta ctatgaaacc aaccaataaa gcagattttc
    17041 aacatgtgat caatgcagct ggctactcta tgaaggggtt aaaagccgct tatattaatg
    17101 aagccgcctt tcgccaagaa atttggtgcg ctactatttt attccctcta gggcttattc
    17161 taggcgaaac caatattgaa aaagcattat tagtaggcac agttttactt gttttagtta
    17221 cagaactact caatagtgcg gttgaatctg ttgttgaccg cattggggct gatttccacg
    17281 agctttctgg tagagctaaa gatattggct ctgcagctgt atttatggcg atggttttac
    17341 tcgctataac ttggatatta attttgtttt gttaactcta tttttcaacc aacactctta
    17401 ctgaggtatt tatgcgctta aaaaaaacat tactttcggt agcactactt agtgcaacat
    17461 cagcaatggc ctacgagaca ggtaaaacat atcgttttac ggttttacat actaacgata
    17521 tacacggtca cttttggcaa agtgataaag gcgaatacgg tctcgctgct caaaaaacat
    17581 tggttgataa tattcgcaaa gaggtcgcag aaaaaggtgg ttcggtatta atgctgaatg
    17641 caggtgatgc gaatacaggc gtgccggagt cagatatgca aaatgcacgt cctgatattg
    17701 aaggaatgaa tgcgattggt tacgatgcaa tggttctagg taatcacgaa tttgattacc
    17761 cactgcaaat tctctctatg caagaaaaat gggctaaatt cccctttatt tcggccaatg
    17821 tcattaataa aaaaacggat aaacccattg taaaacctta cgtgatgtta gaacgaggcg
    17881 gactaaaaat tgctgttatc ggattaacaa ccgaagatac cgcaaaatta ggtaatccag
    17941 atgtaacgga aaatgccttt ttcaaaaacc ctattgaaac agcaaaaaac acattagctg
    18001 aaattaatca aaaagaaaaa cccgacgtac gtatcgcttt aacccatatg ggttactatt
    18061 ttgacggtaa acatggtact aatgcaccag gtgatgtaac aatggcccgt accttagaaa
    18121 aaggagcgtt cgatttaatc attggcggtc atacccacga tactgtttgt attgatgaaa
    18181 aaggtcaatt caaagccaaa tatacgccgg gtgaggaatg taaaccagac ttccaaaacg
    18241 gtacttggat tgtgcaagcc ggcgaatggg gcaaatttgt tggacgggct gattttgagt
    18301 ttaaagatgg aaaaactacc ttggtaaaat atgaattaat tccggtcaac ttaaaaaaaa
    18361 ccattaaaaa agaagacggt actaaagaat accgacttta ccagcctgaa atcaaagccg
    18421 atgaagcaac ctatacctta ttgaaaaaat atcaagatga gggcgataaa aagcttaata
    18481 ttgaagttgg tcgtgtgaaa gggttattag acggcaaacg tgagcatatt cgcttccaac
    18541 aaaccaatct cggtcgttta attgcacaat cgcaaatgga aagggtaaaa gccgatatcg
    18601 ggattatgaa ctccggcggt attcgtacct cccttgaaga aggtgtaata acttataaac
    18661 atattctgac tgttcaacct ttcggtaata tgattgcaac ggtggattta aaaggacaag
    18721 aattgttaga taccctcacc actattgctc tgaaagaagt tgatactggc gcttatcctc
    18781 aatttgccgg catttcaatg gtagtcgacc gtacagcaaa aactatttct gatgttaaaa
    18841 tcggtggcaa accttttgat aaagagaaaa cttataaagt ttctctgcct gactatgttg
    18901 cggctggtgg cgatggctac ccattactga aaaaacaccc tagctatgtc aatacaggct
    18961 ttattgatgc cgaaatgttg aaaaaattct tcgaagaaaa taaaattatt gaggcagata
    19021 aatacgatcc aaaaaaccag atcattttca aatagagcta tagaaaatga aactcactct
    19081 ggcagtctct gctcaaaaga gctgtggctg cctgtagatt gttttcttac tttcaagtgt
    19141 tataggaaat cgataaaaat ccctaccaaa ttaattcatc accatatgat gaaaattaaa
    19201 aaacctctgg caatactttg ccaaaagttt ttgactttta tagggtaata tcact
//
LOCUS       DANKBV010000038        18037 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.038, whole genome shotgun sequence.
ACCESSION   DANKBV010000038 DANKBV010000000
VERSION     DANKBV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18037
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.038"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..204)
                     /locus_tag="PWY43_001053"
     CDS             complement(<1..204)
                     /locus_tag="PWY43_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL2135943.1"
                     /translation="MNRDIRWKQRFDNYQKSVSYLQAEAEKYADTDIDVIKKGIIQSF
                     EITHELAWKLMQDILKWEGEVDIY"
     gene            complement(206..451)
                     /gene="nqrM"
                     /locus_tag="PWY43_001054"
     CDS             complement(206..451)
                     /gene="nqrM"
                     /locus_tag="PWY43_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HDL2135944.1"
                     /translation="METVLLTFGFFVAVIFAMSIGFIVKGKTIKGSCGGITALGMKKM
                     CDCEEPCDNLKSKIADGTADPEEVARFNKEPQFYEVK"
     gene            complement(464..1498)
                     /locus_tag="PWY43_001055"
     CDS             complement(464..1498)
                     /locus_tag="PWY43_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2135945.1"
                     /translation="MKKFFLLLIAFFTLSACEKAPEQITLSGKTMGTTYTVKYIDNGE
                     VENLISPEKMKNELDGLLIEVNNQMSTYQQDSEISRFNQAKQANQAVEISQDFAKVVQ
                     EAIRLNKVTEGALDVTVGPLVNLWGFGPDKRLNKVPSDEQIAERSAYVGIDKILLEMS
                     SKPKLTKSAANLYLDLSSIAKGFGVDKLAEHLETIGVSNYLVEIGGELRGKGKNLQGL
                     DWRIAIEQPVMTQGQAAQITVPLHNLAMATSGNYRNYFEDEKGNRLSHIIDPKQLRPV
                     NHNLASITVFAPTTMTADGLSTGLFVLGAEKALAVAEREKLAIFLIVKNGDKFETQMS
                     SEFSKLIQSQ"
     gene            complement(1547..1840)
                     /locus_tag="PWY43_001056"
     CDS             complement(1547..1840)
                     /locus_tag="PWY43_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2135946.1"
                     /translation="MIDLTESQRQQVLDIFKKYLPDTPVWVFGSRIKGTAKAYSDLDL
                     ALITKQPLTIRQQTELELAFTDSDLPFTVDLIDWASCNESFKQIILQRYEVLI"
     gene            complement(1833..2267)
                     /locus_tag="PWY43_001057"
     CDS             complement(1833..2267)
                     /locus_tag="PWY43_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL2135947.1"
                     /translation="MEQLDITALENAFNSLEKTIVSLENQAWFNRQEAIIQDTLIAGA
                     IQKFEFVYELSIKMLKRQLKNIESNDTDIDSTEFRDILRMAAQYGLIESPEVWLDYRK
                     MRNITSHTYDQNKAQQVYNGISNFLESTYFLVKQLQKRNSYD"
     gene            complement(2379..3608)
                     /gene="nqrF"
                     /locus_tag="PWY43_001058"
     CDS             complement(2379..3608)
                     /gene="nqrF"
                     /locus_tag="PWY43_001058"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HDL2135948.1"
                     /translation="MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDP
                     EKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKK
                     EAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
                     VPFRAGGYIQIEAEPHTVHYKDFDIPKEYHEDWDKFDLWRYTSKVDEHIIRAYSMASY
                     PEEKGIIMLNVRIATPPPRNPDVPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAE
                     MVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSEREMFYVEDFDGLQAENDNF
                     KWYVALSDPLPGDREDYFRGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGML
                     KSLGVEDENILLDDFGG"
     gene            complement(3619..4215)
                     /gene="nqrE"
                     /locus_tag="PWY43_001059"
     CDS             complement(3619..4215)
                     /gene="nqrE"
                     /locus_tag="PWY43_001059"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005529392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HDL2135949.1"
                     /translation="MEHLLSIFVKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGIA
                     VIVVLGIAVPANQLVYTHVLKEGALVEGVDLSFLNFITFIGVIAALVQILEMILDKFF
                     PALYSALGIFLPLITVNCAIFGGVSFMVQREYNFVESVVYGVGAGTGWMLAIVALAGL
                     TEKMKYSDVPAGLRGLGITFITVGLMALGFMSFSGIQL"
     gene            complement(4217..4843)
                     /locus_tag="PWY43_001060"
     CDS             complement(4217..4843)
                     /locus_tag="PWY43_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HDL2135950.1"
                     /translation="MADNNNLKKLLLSPIVDNNPIALQILGICSALAVTTQLQTAVVM
                     AIAVSLVTAFSSMFISMIRNYIPNSIRIIVQMAIIASLVILVDQILRAYAYDLSKQLS
                     VFVGLIITNCIVMGRAEAFAMKSGPVESFVDGIGNGLGYGAMLIIVAFLRELIGSGKI
                     FGITILETVQNGGWYQANGLFLLAPSAFFIIGFVIWAIRTWKPEQVEK"
     gene            complement(4843..5616)
                     /locus_tag="PWY43_001061"
     CDS             complement(4843..5616)
                     /locus_tag="PWY43_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDL2135951.1"
                     /translation="MAKFNKDSVGGTLTVVVLLSLICSLIVAGAAVMLKPTQDIQKQL
                     DKQKNILQAAGLLQKDTNVQETYTKFIEPKIVDLATGDYVEGVTGFDARAAAKDPAQN
                     VVIAPEDDKANIKTRAKYAEVYLVKDESGKTTQVVLPMHGNGLWSIMYGFVAVQPDAN
                     TVNGITYYEQGETAGLGGEIVNPNWQKNFVGKKLYNEQNQVALRVGKGASADKDHGVD
                     GLSGATLTSNGVDGSFKYWFGQNGFGPYLAKFKAAAGAN"
     gene            complement(5609..6844)
                     /locus_tag="PWY43_001062"
     CDS             complement(5609..6844)
                     /locus_tag="PWY43_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HDL2135952.1"
                     /translation="MGLKNLFEKMEPAFHKGGKYEKWYTLFEAAYTIFYTPGTVTRKD
                     AHVRDAIDSKRMMLIVWLALFPAMFYGMYNVGAQGILAALKMGTLTDLVANNWHYSLA
                     DSLGSVADAGWGTKMLLGATYFLPIYLTVFAVGGFWEVVFAMVRKHEINEGFFVTSIL
                     LALIVPPTLPLWQAALATTFGVVVAKEVFGGVGKNFMNPALAGRAFLFFAYPGQISGD
                     AVWVAADGFSGATALSQWAVGGQGALQHVATGETITWMDAFLGNIPGSIGETSTLMLI
                     IGAAIIVFARIASWRIIAGVMIGMAATATLFNVIGSDTNPLFSMPWHWHLVLGGFAIG
                     MFFMATDPVSAAFTNKGKWAYGILIGFMCVLIRVVNPAYPEGMMLAILFANLFAPIFD
                     YLVVQGNIKRRLARVANNG"
     gene            complement(6847..8205)
                     /locus_tag="PWY43_001063"
     CDS             complement(6847..8205)
                     /locus_tag="PWY43_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HDL2135953.1"
                     /translation="MITIKKGLDLPIAGKPEQVIHNGNTVTEVAMLGEEYVGMRPSMK
                     VREGDVVKKGQVLFEDKKNPGVVFTAPASGTVTAINRGEKRVLQSVVIKVEGDEQITF
                     ARYEASQLASLTSEQVKQNLVESGLWTAFRTRPFSKVPALDAEPSSIFVNAMDTNPLA
                     ANPEVIIAEHQQAFFDGLTVLSRLFESKKVIHLCGSADSNILNAAQSRQINNVETNRF
                     KGPHPAGLSGTHIHFIDPVGATKQVWYLNYQDVIAIGKLFTTGELFTDRVVALAGPQV
                     KNPRLVRTRLGANLSQLTANELNAGENRVISGSVLSGAIATGVHDYLGRYALQVSALL
                     EGREKAFFGMIAPYSDKYSITRTTLGHFAKKLFNFTTAVHGSERAMVPTGAYERVMPL
                     DIIPTLLLRDLAAGDTDSAQALGCLELDEEDLALCTFVCSGKNNWGPLLRQALDKIEK
                     DG"
     gene            8652..9248
                     /gene="nfuA"
                     /locus_tag="PWY43_001064"
     CDS             8652..9248
                     /gene="nfuA"
                     /locus_tag="PWY43_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDL2135954.1"
                     /translation="MEEQTIHISISDAAQAHFARLLEQQEEGTNIRIFVVNPGTPGAE
                     CGVSYCPPNAVEETDTEFKFNSFSAFVDDISLPFLDEAEIDYVTDPMGSQLTLKAPNA
                     KMRKVADDAPFIERLDYVIQTQVNPQLASHGGKVTLIEITEDKYAVLQFGGGCNGCSM
                     VDVTLKEGIEKQLLAMFPDELVGVKDITEHQHGEHSYY"
     gene            complement(9417..11054)
                     /gene="pyrG"
                     /locus_tag="PWY43_001065"
     CDS             complement(9417..11054)
                     /gene="pyrG"
                     /locus_tag="PWY43_001065"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDL2135955.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPY
                     INVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRK
                     ERRGDYLGATIQVIPHITNEIKARVLEGGKGRDVAIVEVGGTVGDIESLPFLEALRQL
                     AVDIGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPE
                     NEKKKIALFCNVPERAVISLKDVDSIYRIPELLKSQGLDSFVCDRFRLDCKEADLSEW
                     EQVLYRQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTHRLTVNIKYIDSQDIE
                     TKGTELLAGLDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARN
                     VAGLTEANSSEFDKDCSQPVIGLITEWQDAEGNVETRSDESDLGGTMRLGSQQCHLIE
                     GSKARELYGAETIVERHRHRYEVNNKLLPQIEAAGLKVSGLSADRKLVEIIEVPNHPW
                     FIAAQFHPEFTSTPRDGHPLFAGFVKAAKDHQESRKA"
     gene            11318..12367
                     /gene="ansB"
                     /locus_tag="PWY43_001066"
     CDS             11318..12367
                     /gene="ansB"
                     /locus_tag="PWY43_001066"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDL2135956.1"
                     /translation="MKIKKLLLPTLLSIATMPIQAADLPNITILATGGTIAGSQKNTA
                     TSAYQAGQLNIETLIEAVPDIKKLANIKGEQIVKIGSQDMSDEVWLKLAKAINQQCPN
                     TDGFVITHGTDTMEETVYFLDMTVKCEKPVVLVGAMRPATEKSADGVLNLYNSVVVAK
                     DSKSSGKGVLVAMNDIVLGARDVTKTSTTSVQTFNSPNFGALGYIHNSKVDYERSPES
                     KHTVNTPFKVDELTELPKVGIIYAYSNMPTEPLQALLDASYQGIVVAGVGNGNMNAAN
                     LALLEEAAKKGITVVRSSRVPTGYTTRDAEIDDSKYGFIASGTLNPQKARVLLQLALT
                     QTNDIKTIQQYFEDF"
     gene            complement(12441..13085)
                     /locus_tag="PWY43_001067"
     CDS             complement(12441..13085)
                     /locus_tag="PWY43_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135957.1"
                     /translation="MRIWQKLASVIVVTLGLISNASAENFKLKYSSNYLMPAYINFNN
                     GGGQYNIQAKINVPLYNIVFSAKGTEKNEQFHMLSYRDTRNGKTYAMAEIDSKTVKYG
                     KVKEPLKEEALTMPTYDLFTTAFQLSYYDKLPSSFQTTNGKKLYPTPNVQLRKAQKEV
                     KVGGKTYQEITYKFRTSDKKDITVKKFEGEKFPRYIAYSRDGDNYELTFDSFVK"
     gene            complement(13086..14069)
                     /gene="cysB"
                     /locus_tag="PWY43_001068"
     CDS             complement(13086..14069)
                     /gene="cysB"
                     /locus_tag="PWY43_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDL2135958.1"
                     /translation="MKLQQLRYIVEIVNQNLNITEAAEVLYTSQPGISKQVRLLESEL
                     GINIFERNGKHVKGLTPAGEKIVAIARELLVKAQSIKSVAEEQTRPNKGILRIATTNT
                     QARYMLPSVIEKFKARYPEVSLHLHQGSPNQIYDALVAGEVDLAITTEAQYLFDEAVL
                     LPCYWWNRSVIVKPEHPLAAFVNQSKNLTVEQLGRYDLITYTFGFTGQSDLDHAFNKE
                     GILPNIVFTATDADVIKTYVRLGLGVGIMASMAHTETDKDLVAINASHLFQPSMTQIA
                     FKRGTFLRNYMYDFINYFSPHLTRVQVEKAEQLRDNNAISRLFESISLEEK"
     gene            complement(14066..14749)
                     /gene="yjjG"
                     /locus_tag="PWY43_001069"
     CDS             complement(14066..14749)
                     /gene="yjjG"
                     /locus_tag="PWY43_001069"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDL2135959.1"
                     /translation="MKYQWVLFDADETLFSFNSYLGLKAMLARYQIDFSEQDYQDFQA
                     VNKPLWVAYQNNEITAQDIQTRRFAKLSAQTGVDPLQLNQELMAEMAFVSQPLEGVMD
                     MLNALYGKVKMGIITNGFTDLQQKRLDNTQTSHFFDIVVISEQIGAAKPDRQVFDYAF
                     ALMDEFDRTKVLMVGDTLASDILGGNNAGIDTCWLNLLSKENDTDIKPTYEVHTMQQI
                     IAIVEGKSL"
     gene            complement(14825..15718)
                     /locus_tag="PWY43_001070"
     CDS             complement(14825..15718)
                     /locus_tag="PWY43_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HDL2135960.1"
                     /translation="MNKKLPPLNALKAFESAARHLNFTKAAEDLFVTQAAVSHQIKLL
                     EDFLGASLFHRRNRLLELTELGAQYFYEVQPLLEQIALATDKIRQQSSRQILTISVPQ
                     TFGMHWLVPRLADFHEKYPEVEVRIKGVDQDEGLLGKETDVAIYYGKGDWQGLESIRL
                     TESRIVILASPEYLSQNTIHLPADLAGKHLLHSSTHNKWKRMVEHLHIEDIVDAETGS
                     IFSHTFMVLQAAMHQQGIAIANQLLAQHEIEKGTLIEPFPTTLFDEKSFYVVYPSQSA
                     QVQKVQFFVEWIQQKMKECCA"
     gene            16164..17201
                     /gene="rsgA"
                     /locus_tag="PWY43_001071"
     CDS             16164..17201
                     /gene="rsgA"
                     /locus_tag="PWY43_001071"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDL2135961.1"
                     /translation="MSKPKLTHNQQRRIQSNHHKRINKKEIEWQDEMLGEMQQGIVVT
                     RHVKHADVETAEGQIFRCNIRRTLKNVVVGDQVSWRKGSEQLQGISGVIEAVFPRKNE
                     LTRPDYYDGIKVMASNIDQIIIVSAVLPTLSLNIIDRYLVICETANIPALIVLNKIDL
                     LNESERQAVKEQLKIYEEIGYETLCLSADTGENMQALHQYLAKGTSIFVGQSGVGKSS
                     LINQLLPEVNALTGSVSDNSGLGQHTTTASRLYHLPQGGQLIDSPGIREFGLWHLEPE
                     QITLGYREFQSVLGTCKFRDCKHKTDPGCALRQAVENGEISAIRFENYHRLIESLSET
                     KSQRHFRKEEV"
     gene            17198..17797
                     /gene="rnhB"
                     /locus_tag="PWY43_001072"
     CDS             17198..17797
                     /gene="rnhB"
                     /locus_tag="PWY43_001072"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL2135962.1"
                     /translation="MMNEFIYPNARLIAGVDEVGRGPLVGAVVTAAVILDPNNPIQGL
                     TDSKKLSEKKRQALAEEIKAKALSWSLGRAEPTEIDELNILNATMLAMQRAVAGLHIQ
                     PDFVLIDGNRPPKLPMPAQAVVKGDSLVAEISAASIIAKVARDQEMDELDQQFPQYGF
                     AQHKGYPTKLHFEKLAEFGATPYHRKSFAPVRKVLGLID"
     gene            17810..>18037
                     /locus_tag="PWY43_001073"
     CDS             17810..>18037
                     /locus_tag="PWY43_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDL2135963.1"
                     /translation="MTTEYQETIFTKIINKEIPANIVYQDELVTAFRDIAPQAPTHIL
                     IIPNKFIPTVNHVSNEDELALGRLFTVAAKLA"
BASE COUNT         5478 a         3910 c         3290 g         5359 t
ORIGIN      
        1 ataaatatca acttcacctt cccattttaa aatatcttgc attaacttcc acgctaactc
       61 gtgtgtaatc tcaaaactct ggataattcc ttttttaatt acatcaatgt cggtatcagc
      121 gtatttttct gcttctgctt gaaggtaact tactgatttt tgatagttat caaaccgctg
      181 tttccagcga atatctctat tcatattatt tcacttcata aaattgtggc tctttattaa
      241 aacgtgccac ttcttcaggg tcggctgtgc cgtctgcgat tttagatttt aggttatcgc
      301 aaggttcttc gcagtcgcac atttttttca tacctaaagc ggtgataccg ccacagcttc
      361 ctttgatggt tttgcctttc acgataaaac caatcgacat cgcaaaaatt acggctacaa
      421 aaaagccaaa ggttaataat acagtttcca tttttcactc caactattgt gattgaataa
      481 gtttgctaaa ttcgctcgac atttgtgtct caaatttatc gccgttttta acaattaaaa
      541 aaatagctaa tttttcacgc tctgccactg ctaatgcttt ttccgctcct aaaacaaata
      601 aaccggttga taagccgtct gccgtcattg tagttggggc aaatacggtg atagaggcta
      661 aattatggtt taccggacgt aactgttttg ggtcgataat atgtgaaaga cggttgcctt
      721 tttcatcttc aaaataatta cgataattgc ccgaagttgc catcgccaaa ttgtgcaacg
      781 gcacggtaat ttgtgcggct tgaccttgtg tcattaccgg ctgctcgatt gcaatccgcc
      841 aatctaagcc ttgtaaattt tttcctttgc ctcgtaattc gccaccaatt tcaactaaat
      901 agttgctcac acctatagtt tcaagatgct cggctaattt atctacaccg aaaccctttg
      961 caatagacga taaatctaga taaagattag ctgctgactt ggtgagcttg ggtttactac
     1021 tcatttcaag caaaatttta tcaatgccga cataagcaga acgctctgca atttgttcat
     1081 cagacggcac tttatttaag cgtttatccg gcccgaagcc ccacaagttc accaatggac
     1141 caacggttac atctaaagcc ccttcggtta ctttatttaa acggattgcc tcttgtacca
     1201 cttttgcaaa atcctgcgaa atttcgaccg cttgatttgc ctgctttgct tgattaaaac
     1261 ggctaatttc cgaatcttgt tggtaggtgg acatctgatt attcacctca atcaataagc
     1321 catccagctc atttttcatt ttttcaggcg aaatcaaatt ctccacttca ccattatcaa
     1381 tatattttac ggtgtaagtc gttcccatgg ttttgcctga cagcgtaatt tgctcagggg
     1441 ctttttcaca agcacttaaa gtaaaaaaag caattaaaag aagaaaaaat tttttcattg
     1501 gtgtcacaaa agttaaaaat tggggaaatt atagcatatt tagcgattaa ataagcactt
     1561 catatcgctg taaaataatt tgtttaaacg attcgttgca acttgcccag tcaatcagat
     1621 ctactgtgaa aggtaggtca gaatccgtaa acgcaagctc taactctgtt tgttgccgaa
     1681 ttgtgagcgg ttgtttggtg attaatgcta aatcaagatc cgaataagct ttggctgtgc
     1741 ctttaatgcg agagccaaaa acccaaacgg gtgtatcagg caagtatttt ttgaaaatat
     1801 ccaacacctg ttggcgttgt gactcagtta aatcaatcat agctattgcg tttttggagc
     1861 tgtttaacta aaaaatacgt actttccaga aagtttgaaa ttccgttata aacctgctgt
     1921 gctttatttt gatcataagt atgcgaagta atatttcgca ttttgcgata atccagccaa
     1981 acttccggtg attcaatcag cccatattga gcagccattc gcaaaatatc acggaactcg
     2041 gtgctatcaa tatcagtgtc atttgactca atgtttttga gctgacgctt gagcattttt
     2101 atcgacaatt cgtaaacaaa ttcaaatttt tggattgctc ctgcaatcaa agtgtcttga
     2161 ataattgctt cttgccggtt aaaccacgct tgattctcaa ggctgacaat cgttttctca
     2221 agggaattga aggcattttc aagtgccgta atatccagtt gttccatttg cttcccctta
     2281 aaattcacat tattaaatta ccaaaactta ataatataaa ttaaatcaag cggtcaattt
     2341 tgcaaatttg accgcttgta gtcagaagtt aaggtttatc aaccaccaaa gtcatctaat
     2401 aagatgtttt cgtcttctac gcctaaactc ttcagcatac cgattaccgc agcattcatc
     2461 accggtggac cacacatata gtattcgcag tcttcaggtg cttcgtggtt tttgaggtag
     2521 ttctcataaa gcacgttatg aataaagcca cggaagtaat cttctctgtc gcccggtaat
     2581 ggatcagaaa gtgccacata ccatttgaag ttatcgtttt ctgcttgaag accatcaaaa
     2641 tcttccacat agaacatttc acgctcagaa cgagcaccat accagaatga cattttacgt
     2701 ttagatttta aacgttttaa ttggtcgaag atatgtgaac gcattggtgc catacctgca
     2761 ccaccaccga taaagaccat ttccgcatcg gtatctttag cgaagaactc accgaatgga
     2821 cctgaaattg ttactttatc acccggtttt aatgaccaaa tgtaggaaga catttgaccc
     2881 ggcggtacgt ccggattgcg tggtggaggt gtagcaatac gcacgttaag cataataatg
     2941 cctttctctt ccggatatga agccattgag tatgcacgaa taatatgctc gtccactttt
     3001 gaagtataac gccataagtc aaatttatcc cagtcttcgt ggtactcttt tggaatatcg
     3061 aagtctttat aatgaacggt atgaggttca gcttcaattt ggatataacc acccgcacgg
     3121 aacggtactt cttcgccttc aggaatttga agtttaagct ctttgatgaa ggttgctttg
     3181 ttatcgtttg agataacggt acattcccat tttttcacac caaagatctc ttccggaagt
     3241 tcaacgtcca ttgaagattt cacgtttacc tgacaagcta aacgccagcc ttctttcgct
     3301 tctttcttag aaatgtgcga taactcagtt ggaagaattt caccaccgcc tgatttcact
     3361 tgtactttac attgaccgca agagccaccg cccccacaag ctgacgaaac gaaaatccct
     3421 ttacttgcta aagcaccgag taattttcca ccagccggta gcgtaatgcc tttttccgga
     3481 tcgttgttga ttgagatggt aatatcacct gaatcaacta attttgattt agcgaaaaga
     3541 atgatcaccg caagcactaa taccagagcc gtaaatgcga taataccgaa aataaaatta
     3601 ctatccacga tatgctcctt ataattgaat gcctgagaat gacataaagc ctaacgccat
     3661 taagcctacc gtaataaagg taatacctaa accacgtaag cctgccggca cgtcggaata
     3721 tttcattttc tcggttaaac ctgcaagggc aacgatggct aacatccagc ctgtacctgc
     3781 acctacgcca taaaccacag attctacaaa gttatactca cgttgtacca taaacgatac
     3841 accaccaaaa atcgcacagt ttacggtgat aagtggtaag aagataccta atgcactata
     3901 aagtgccggg aaaaacttgt ctaaaatcat ttccagaatt tgaacaagtg ccgcaataac
     3961 cccgatgaaa gtgataaagt ttaagaagct taaatccaca ccttctacta atgcaccctc
     4021 ttttaatacg tgggtataaa ctaattggtt agctggaact gcaatgccaa gcactacaat
     4081 taccgcaata cctaaaccaa aagcagttga aactttctta gacaccgcaa ggaatgtaca
     4141 cataccaagg aagaaagaaa gtgccatatt ttcaataaat acggacttaa caaagatact
     4201 cagaagatgt tccatcttat ttctccactt gttccggttt ccacgttcta atcgcccaga
     4261 tcacaaatcc gataataaag aacgcacttg gtgctaataa gaataaaccg tttgcttgat
     4321 accaaccgcc attttgaaca gtttcaagaa tggtgatacc gaaaatttta cctgaaccga
     4381 taagttcacg taaaaaggca acgataatta acattgcacc gtagcctaaa ccgttaccga
     4441 taccatcaac aaaactttca accgggcctg atttcatcgc gaatgcttct gcacgcccca
     4501 ttacgataca gttggtaata ataaggccta cgaataccga taattgtttt gacagatcgt
     4561 aagcataagc gcgtaagatt tggtcaacca aaattactaa tgatgcaata atcgccattt
     4621 gcacaatgat acggatacta tttggaatat agttacgaat cattgaaatg aacatacttg
     4681 agaatgcagt aactaaactt accgcaatcg ccattactac tgctgtttgt aattgagtcg
     4741 ttaccgctaa cgcagaacaa atacctaaga tctgtaatgc gatcgggttg ttatcgacaa
     4801 ttggcgataa caataatttt ttaagattgt tattgtctgc cattagttag ctcctgccgc
     4861 tgctttaaat tttgctaaat atggaccaaa accattttga ccaaaccagt atttgaatga
     4921 accatcaaca ccgttcgagg ttaatgttgc accagataaa ccatcaacac cgtgatcttt
     4981 atctgcagat gcccctttac ccactcttaa cgcaacttgg ttttgctcgt tatacagttt
     5041 tttacctaca aagtttttct gccagttcgg gtttacaatt tcgccaccta agcctgccgt
     5101 ttcaccttgt tcatagtaag taataccgtt aacggtattt gcatcaggtt gtaccgcaac
     5161 gaaaccgtac ataattgacc ataagccgtt accgtgcatt ggtaatacta cttgagtcgt
     5221 tttgccagat tcatctttta ctaaataaac ttcagcatat ttagcacgcg ttttgatatt
     5281 cgccttatca tcttcaggtg cgataactac attttgagca ggatctttag ctgctgcacg
     5341 agcatcaaaa ccggttacac cctcaacgta atcacctgtt gctaaatcga caattttcgg
     5401 ctcaataaat ttggtatagg tttcttgcac attggtatct ttttgcaata aaccggccgc
     5461 ttgtaagatg tttttctgtt tatcaagttg tttctgaata tcttgtgttg gtttaagcat
     5521 tactgccgca cctgccacaa tcaaagaaca aattaaactt aataaaacta caacggttag
     5581 cgttccgcca acgctatctt tattaaattt agccattgtt tgctaccctc gctaatctgc
     5641 gtttgatatt cccttgaact actaaatagt caaagattgg agcaaataag ttagcaaata
     5701 agatcgctaa catcatacct tcagggtatg ctgggtttac cacacggatt aatacacaca
     5761 taaagccgat taagataccg tatgcccatt tacctttgtt ggtaaatgca gctgataccg
     5821 ggtcggttgc cataaagaac ataccgatcg caaaaccgcc taatactaaa tgccaatgcc
     5881 aaggcataga gaataatggg ttggtatcag aaccgattac attaaataat gtcgcagttg
     5941 ccgccatacc aatcattacg ccggcaataa ttcgccaaga agcaattcgg gcaaatacaa
     6001 tgattgcagc accaataatc aacattaagg tagaggtctc gccgatagaa cccggaatat
     6061 tacctaagaa cgcatccatc caagtaatgg tttcaccggt tgcaacgtgc tgtaatgcac
     6121 cttgaccacc aactgcccat tgagaaagtg cagttgcacc tgagaagccg tctgcggcaa
     6181 cccatactgc atcacctgaa atttgacccg ggtaagcaaa gaacaagaaa gcacgaccgg
     6241 ctaacgcagg gttcataaag tttttaccca cgccaccgaa cacttctttt gcgaccacaa
     6301 caccaaaggt agttgcaagg gctgcttgcc ataacggtaa tgttggagga acaattaatg
     6361 ctagtaagat agaggttaca aagaaacctt cgttgatctc gtgcttacgc accattgcaa
     6421 acaccacttc ccagaaacca cccactgcaa atactgttaa gtagattggt aggaaatagg
     6481 ttgcccccag caacatttta gttccccagc ctgcatcagc tacagagcct aaactatcgg
     6541 ctaagctgta gtgccagttg ttagcaacta aatccgttaa tgttcccatt ttgagtgccg
     6601 ctagaatacc ctgtgcacca acattgtaca ttccgtagaa catcgcaggg aataacgcta
     6661 accatacaat taacatcata cgcttagagt caatcgcatc acgcacgtga gcatctttac
     6721 gagtgaccgt acccggtgta tagaaaatcg tataggcagc ttcaaatagc gtgtaccatt
     6781 tctcatactt tccaccttta tggaaagcgg gttccatttt ttcaaaaaga tttttcaaac
     6841 ccatttttaa ccgtccttct cgatcttatc taacgcttga cgtaataatg gtccccagtt
     6901 gtttttaccg gaacacacaa aagtacataa cgctaaatct tcttcgtcta actctaagca
     6961 gcctaaagct tgagcggagt ctgtatctcc agctgctaaa tcacgcaata agagtgttgg
     7021 aatgatatcc aacggcatta cacgctcata agcccctgtt ggtaccattg cacgctcact
     7081 accgtgaact gctgttgtga agttaaataa tttcttcgca aaatgcccta aagtagtacg
     7141 cgtaattgaa tacttatcag agtaaggagc aatcataccg aagaacgctt tctcacgccc
     7201 ttctaataaa gcagacactt gtaatgcgta acgacctaaa tagtcgtgta ctcccgttgc
     7261 gatagcacca cttaacacag aacctgaaat gacacggttt tcacccgcat ttaattcgtt
     7321 ggctgttaat tgtgaaagat tcgcccctaa acgagtacgc accaaacgtg gatttttcac
     7381 ttgtggcccg gctaatgcga ctacacggtc agtaaatagc tcaccggtcg taaataattt
     7441 accgatagca attacatctt ggtaatttaa ataccaaact tgtttagttg cacctaccgg
     7501 gtcaataaag tgaatatgcg taccagaaag acctgcaggg tgcggacctt taaagcggtt
     7561 agtttccaca ttattgatct gacggctttg tgccgcattt aaaatatttg agtctgcact
     7621 gccacagaga tgaatcactt ttttactttc aaataaacga cttaatacag ttaagccatc
     7681 aaagaaagct tgttggtgtt ctgcaataat cacttccgga tttgctgcaa gtggattggt
     7741 atccatcgca ttgacaaaaa tagaggaagg ttctgcatct aatgcaggca ctttgctaaa
     7801 cggacgtgta cggaaagccg tccataagcc tgactctaca aggttttgtt ttacttgctc
     7861 tgaagttaat gaagctaatt ggctggcttc ataacgagca aaggtaattt gctcatcacc
     7921 ctcaacttta attactaccg attgaagaac acgcttctcg ccacggttaa ttgccgttac
     7981 ggtaccactt gcaggggcag taaataccac gcccggattc tttttatctt caaaaagaac
     8041 ctgacctttc tttactacat cgccttcacg cactttcatt gaaggacgca tacccacata
     8101 ttcttcacca agcatagcaa cttcagtaac ggtattaccg ttatggatta cttgctcagg
     8161 ttttcccgca atcggaagat ctaagccttt tttgattgta atcatattgt ttgcactact
     8221 tttcttttgg gttaaaaaat atgattgcgc taacgcaatc gcttctattg agccgcagct
     8281 ttacttaaaa ataaacaatg gtcccccacc tatttttaaa agccacatta agttctcaaa
     8341 tctgtgatca acctttatct tttcttaaca aaaggttaac taaaaataat cttcttattt
     8401 tacctaaaaa gctccccttc tggctaggaa acccccctat tttttgcata ttcccgcggt
     8461 aaaatttgaa ataaatcaag atttcaattc cctcttaaca taaattaacc ataattagtt
     8521 aatccaattt cccttcaagc ggttaaattt tattaacaat ttgcaaaaat ttttttagct
     8581 aggcaatttt gaaattcagg cgtaaaatag ttgataaatt ttgtaaaata ttaaaaaaca
     8641 ggataattta aatggaagaa caaaccattc atatttcaat atcagatgca gcacaagccc
     8701 attttgccag actattagaa cagcaagaag agggaactaa tatccgtatt tttgtggtca
     8761 atcccggcac accgggggca gaatgcggtg tctcttactg cccacccaat gcagttgaag
     8821 agacggatac cgaatttaaa tttaacagtt tctctgcatt tgtcgatgat attagcttac
     8881 cgtttttaga cgaagcggaa attgattatg taaccgaccc aatgggttca caacttaccc
     8941 tcaaagcccc taacgccaaa atgcgtaaag tggctgatga tgcaccgttt atcgagcgtt
     9001 tagattatgt gattcaaacc caagtcaatc cacaacttgc cagccacggc ggtaaagtga
     9061 ccttaattga aatcaccgaa gataaatatg ccgtgctaca attcggcggt ggttgtaacg
     9121 gttgctcaat ggtggatgtg accttaaaag agggaattga gaaacagctt ctggcaatgt
     9181 tccctgatga attagttggt gtgaaagata ttaccgaaca ccaacacggt gaacattctt
     9241 actattaaaa atactaaaag ggataaatca tttgatttat ccctttctta taattaatga
     9301 gcaagcggtc aaatttactc gctttcgctc gcacttcgtg ccatcagcaa agccgatgtt
     9361 caaacgcaag cgtttgtaca aaaaaatgag taaattcgac cgcttgttaa gtttgattaa
     9421 gccttacgac tctcttggtg atctttcgca gctttcacaa aacctgcaaa taatggatga
     9481 ccatcacgcg gagttgaggt aaattccgga tggaattgtg ctgctataaa ccaagggtga
     9541 tttggcactt caataatttc cactaattta cgatctgccg ataaaccgct cacttttaag
     9601 cctgccgctt caatttgtgg taacagctta ttgtttactt cgtaacggtg gcgatgacgt
     9661 tcaacaatcg tttccgcacc atataactca cgagcttttg agccttcaat taaatggcat
     9721 tgttgtgagc ctaaacgcat tgtaccacct aaatctgact catcgctgcg ggtttccaca
     9781 ttcccttccg catcttgcca ttcggtaatt aagccaatca ccggctgtga gcaatcttta
     9841 tcaaattcag aagaatttgc ctcggttaaa cctgccacat tacgggcata ttcaattagg
     9901 gcgatctgca tacctaaaca gatacctaag taaggaatct tgttttcacg ggcatattgt
     9961 gcggttcgaa ttttgccttc cacgccacga tagccaaagc cgcccggcac aagaatgccg
    10021 tctaagcctg ccaataactc tgtgcctttg gtttcaatat cttgtgaatc aatgtattta
    10081 atattcactg ttaaacgatg cgttaaacct gcgtgtttca aagcctcatt caccgattta
    10141 tacgcatcag gtaattccac atattttccg accataccga tagtcacttc gccggttggg
    10201 tttgcttggc ggtatagcac ttgttcccac tctgaaagat cggcttcttt gcaatctaaa
    10261 cggaaacgat cgcacacaaa ggaatctaac ccttgcgatt tcaataattc cggaatgcga
    10321 taaatcgaat ctacatcttt aagcgaaata accgcacgct ccggcacatt acagaaaagg
    10381 gcgatttttt tcttctcatt ttccggaata gcacgatctg aacggcaaat taacacatcc
    10441 ggctgaatac cgattgaaag caactctttt actgaatgct gagtcggctt agttttcact
    10501 tcccctgcgg ttggaatata aggcactaag gttaaatgca taaacaaggt tttttcacga
    10561 ccgatatcca ccgctaattg gcggagtgct tctaagaatg gcagtgattc aatatccccc
    10621 accgtgccac ctacttctac aatggcgaca tcacgccctt taccgccttc taacacacgg
    10681 gctttaatct cgttggtaat atgcggaata acctgaatgg ttgcacctaa atagtcgcca
    10741 cggcgttctt tacgcaacac ttcagagtaa attttaccgc tggtaaagtt gttggctttg
    10801 ctcatttttg aacggataaa acgctcgtag tgacctaaat ccaaatcggt ttccgcaccg
    10861 tcttcggtca caaatacctc cccgtgctga gttggactca ttgtacccgg atcgacattg
    10921 atataaggat caagcttcat aatggttaca tttaaaccac gagcttccag aatagatgct
    10981 aaagaggctg ccgcaatacc cttccctaaa gaagatacaa caccgcctgt gacgaaaata
    11041 taattcgttg tcattgtgat tgcctttgct gattgattag gttggaaatt cagaatatca
    11101 gtttacttcg taaaaaagaa actatcagga cgggaaagta gtttacatca aattacattt
    11161 ttttgctaat ggaaaatcgg caagcagtca gattaattct tttttcaata atgccgacta
    11221 atcttaatta atacactatt atttgcgata gatcagaatt aacctacatg tttatagata
    11281 tcataatacc tgtttttcta atactggaga cgatacaatg aaaatcaaaa aattactact
    11341 gccaactctg ttaagcattg ctactatgcc aattcaagca gccgacctac ctaatattac
    11401 aattcttgca actggaggga caattgccgg tagccaaaaa aatacggcaa cctcagctta
    11461 tcaagctggg cagctaaata ttgaaacact catagaagcc gtcccggata ttaaaaaatt
    11521 agcaaatatc aaaggagaac aaatcgttaa aatcggatct caggatatgt ctgatgaagt
    11581 atggctaaaa ctggcaaaag caattaatca acaatgccca aacacagatg gcttcgttat
    11641 tacgcacggc acagacacta tggaggaaac cgtatacttc cttgatatga ctgtaaaatg
    11701 cgaaaaaccc gtagtattag tgggggccat gcgaccagca actgaaaaaa gtgccgacgg
    11761 cgtattaaat ttatataact cagttgttgt cgccaaagat tctaaatcta gtggcaaagg
    11821 ggtgttggtt gcaatgaatg atattgtatt gggcgctcgc gatgttacaa aaaccagtac
    11881 tacatcagtg caaacattta actcaccaaa ctttggcgca ctaggctata tccataatag
    11941 caaagttgat tatgagcgct ctcctgaaag taaacacacc gtaaacaccc ctttcaaagt
    12001 agatgaatta acggaattgc caaaagtcgg cattatttat gcttattcaa atatgccaac
    12061 cgaaccgctt caagcattat tagatgcgag ttatcaaggc atcgtggttg caggagttgg
    12121 caatggcaat atgaatgcgg ctaatttggc tttacttgaa gaagcagcta aaaaaggaat
    12181 taccgtggtt cgctcgtctc gagtgccaac cggatatact acccgagatg cagaaattga
    12241 tgatagcaag tatggtttta ttgcttccgg cacattaaat cctcaaaaag ctcgagtatt
    12301 actgcagctc gccttaacac aaacaaatga tataaaaact attcaacaat attttgaaga
    12361 tttctaaaaa gtaaataaca agcggtctat tttgcaaaac aaatcacaaa atagaccgct
    12421 tgtctcatta ctaatcttta ttattttaca aagctatcaa aagttagctc ataattgtcg
    12481 ccatcacgac tataggcgat atagcgaggg aatttttcac cttcaaattt tttcaccgta
    12541 atgtcttttt tatcactggt tctaaacttg taggtaattt cttggtaggt tttgccgcct
    12601 acttttacct ctttttgggc tttgcgtagc tgcacattcg gtgttgggta aagtttttta
    12661 ccgttagtag tttgaaaact gctcggtaat ttatcgtaat agctcaactg aaaggctgtg
    12721 gtgaacaaat cataagttgg catagtcagc gcttcttctt ttagtggctc tttcactttg
    12781 ccatatttta ccgttttaga atcaatttct gccatcgcat aagttttgcc gttacgggta
    12841 tctcggtagc tcaacatatg aaattgttcg tttttttctg ttcccttcgc agaaaagaca
    12901 atattatata aaggcacatt aatttttgcc tgaatattat attggccacc accgttatta
    12961 aagttaatgt aggctggcat taaataattt gagctgtatt tcaacttaaa attctccgca
    13021 ctggcgtttg aaatcaagcc caaagtgacc acgattacgc tcgctaactt ctgccaaatt
    13081 ctcatctatt tctcctctaa agatatactt tcgaacaacc ttgaaattgc attgttatct
    13141 cgtaattgtt ccgctttctc cacttgtacc cgtgttaaat gtggcgaaaa ataattaata
    13201 aaatcataca tatagttacg aagaaaagtt cctcgtttaa aggcgatttg cgtcatactc
    13261 ggttgaaaca ggtgacttgc attgatagcg actaagtctt tatccgtttc cgtatgtgcc
    13321 attgatgcca taataccaac ccctaagcct aagcgcacat aagttttgat tacatcagca
    13381 tctgtcgccg taaatacgat attaggcaaa ataccttcct tattgaaggc atgatctaaa
    13441 tccgattgcc ctgtaaaacc aaaagtatag gttatcaaat catatctacc taattgttcg
    13501 acagtcaaat ttttactttg gttcacaaaa gccgcaagag gatgctctgg tttgacaata
    13561 actgaacgat tccaccaata acacggcaat aaaaccgctt catcaaataa atattgagct
    13621 tctgtggtaa tggctaaatc cacttctcca gccaccaaag catcataaat ttgattcggg
    13681 gagccttggt gcaaatgcaa acttacttca ggatagcgag ccttaaactt ctcaattaca
    13741 ctcggtagca tataacgggc ttgggtattg gtggtcgcaa tgcgtaagat gcctttgttg
    13801 ggtcgagttt gttcttcagc aaccgattta atgctctggg cttttaccaa taattcacga
    13861 gcaatcgcca caattttttc accagcaggc gttagcccct taacgtgctt accgttacgc
    13921 tcaaaaatat taattcccaa ctcactttcc aacaaacgaa cttgtttgct aatccccggt
    13981 tgagaagtgt aaagcacctc tgcggcctcg gtaatattca agttctggtt tactatttca
    14041 acgatataac gtaactgttg tagcttcata atgatttccc ctctacaatc gcaataatct
    14101 gttgcatcgt atgcacttcg taagttggct taatatccgt atcattctct ttactaagca
    14161 aatttaacca acaagtatca attccagcat tattacctcc taaaatatca gaagcgaggg
    14221 tatcgcctac cataagcact ttagtacgat caaattcgtc cattaatgca aaagcgtaat
    14281 caaacacttg gcgatcaggc tttgcggcac caatttgttc tgatatcact acaatatcaa
    14341 aaaaatgtga agtttgagta ttatctaaac gtttttgctg caaatcggta aaaccattgg
    14401 tgataatgcc catttttact ttaccatata aagcatttaa catatccatt acaccttcta
    14461 aaggctgact gacgaatgcc atctccgcca ttaactcttg attaagttgt agcggatcta
    14521 cgcctgtttg agcagataat tttgcaaaac gtctggtttg aatatcttga gcagtgatct
    14581 cattattttg gtaagccacc caaagtggtt tatttaccgc ttgaaagtct tgataatctt
    14641 gttcactaaa atcaatttgg taacgagcca gcatcgcttt taaaccaaga taagaattaa
    14701 atgaaaacaa ggtttcatca gcatcaaata atacccattg atatttcata atattccttt
    14761 tttaacttag ataacaagcg gtcaaatttt tctaattttt cgcataccgc tgataaagcc
    14821 acgcttaagc acaacactct ttcatttttt gctgaatcca ctcaacaaaa aattgtactt
    14881 tctgaacttg ggctgattgc gaaggataga caacatagaa cgatttttca tcaaacaatg
    14941 tggttgggaa aggctctatt aaagtacctt tttcaatttc gtgctgtgcc aacaactggt
    15001 tggcaatcgc aatgccttgt tggtgcattg cagcttgaag caccataaaa gtatggctaa
    15061 aaattgagcc agtttccgca tctactatat cttctatatg caaatgttca accattcttt
    15121 tccacttatt atgggtggag gaatgtaata aatgcttacc tgctaaatcc gccggtaaat
    15181 gaatggtatt ttgcgataaa tactcaggcg aagctaaaat aacaatacgg gattccgtca
    15241 aacggataga ttccagacct tgccaatccc ccttaccata ataaatagca acatctgttt
    15301 ctttacctaa taatccctca tcttgatcaa cacccttaat acgtacttcc acttctggat
    15361 atttttcgtg gaaatccgcc aaacgtggga ctaaccaatg cataccaaat gtttgtggta
    15421 cgctaatagt cagaatttga cgactacttt gctgcctaat tttatctgtt gcgagagcaa
    15481 tttgttctaa taatggctga acttcataga aatattgtgc gcctaactca gttaattcca
    15541 gcaaacggtt acggcgatgg aacagggaag cacctaaaaa atcttctaat agtttaattt
    15601 ggtggctcac tgcagcttgt gtgacgaaaa gatcttctgc cgctttagta aaatttaaat
    15661 ggcgtgcagc agattcaaat gctttcaatg cattgagtgg tggaagtttt ttgttcataa
    15721 tttttcaatc taatagatta ctaaacaagc aactctatta ttttttctga tagtttaaat
    15781 aaaaaaaatt cgtttgatgc ttgcaaatag aaataataaa atatccgcat acttagatgg
    15841 atagtttaga atttatttct attcctaaat tttaagtatg atgttgtgtt tgcatattgg
    15901 tctaggaaac tagactagag taacgaaagt tacttgtttc acttcctgta tattttgaac
    15961 cgtattggtt tataaccgcc cactttgggc ggtttttttc tatgtaaaaa aacagcacaa
    16021 aacctagatc gcattctata ttattcgcta cctttattct agcctttctg cttttttatt
    16081 ctttcacttt aatctattat aaaaagaggt tttatagtat gctaaagcct atttttcatt
    16141 tgcaataatt accgagagtt cttttgagta aaccgaaatt aacccataac caacagcgta
    16201 gaatccaatc taaccatcat aagcgtatca acaaaaaaga gattgaatgg caagatgaaa
    16261 tgttaggaga aatgcaacag ggcatcgtcg ttactcgcca cgttaaacac gctgatgttg
    16321 aaaccgcaga ggggcaaatt ttccgctgta atatccgccg tacattgaaa aatgtagtgg
    16381 tgggcgatca ggtttcttgg cgtaaaggta gtgagcaact acaagggatt agtggggtca
    16441 ttgaagccgt cttcccacgt aaaaatgaac ttacccgccc cgattattat gacggtatta
    16501 aggtgatggc atcgaacatc gatcaaatca ttattgtatc agctgtgcta cctacacttt
    16561 cgctgaatat tatcgaccgt tatttagtga tttgcgaaac ggcgaatatt cctgccttaa
    16621 tcgtgctaaa caaaattgat ttattaaacg aaagcgaacg gcaagcagtt aaagagcagc
    16681 tcaaaatcta tgaagagatt ggctatgaaa ccctatgttt atcggctgat acgggtgaaa
    16741 atatgcaggc gttacaccaa tatcttgcta aaggtacgtc tatttttgtc ggacaatccg
    16801 gcgtggggaa atccagccta attaaccaac tgttacctga agtcaatgct ctcactggct
    16861 cagtgagcga taactcaggg ttaggtcagc acaccaccac tgcctctcgc ttataccatt
    16921 taccgcaagg cggacagctg attgactcgc cgggcattcg agaatttggc ttatggcatt
    16981 tagaacctga gcaaatcacc ttaggctatc gagaatttca atcggtttta ggcacgtgca
    17041 aattccgtga ttgcaaacac aaaaccgacc cgggttgtgc cttaagacaa gcggtcgaaa
    17101 atggcgaaat ttctgcaatt cggtttgaaa attaccatcg cttaattgaa agcctgtctg
    17161 aaaccaaaag ccaacgccat ttcagaaaag aagaagtata atgaatgaat ttatttaccc
    17221 taacgcccga ttaatcgccg gtgttgacga agtcggacga ggacctttag tcggagcggt
    17281 tgtgaccgca gccgtgattt tagatccgaa taacccgatt cagggcttaa ccgattccaa
    17341 aaaattatcc gagaaaaaac gccaagcttt ggcagaagag attaaagcca aggctctgag
    17401 ctggtcgcta ggacgtgccg agccgacaga aattgatgag ctgaatattt taaatgcgac
    17461 aatgttggca atgcaacgtg cggttgccgg cttgcatatt cagccggatt ttgtgctaat
    17521 tgacggcaat cgtccgccaa aactgccaat gccggcacaa gcggtggtga aaggagacag
    17581 cttagtggct gaaatcagtg ctgcttcgat tattgccaaa gtcgcccgag atcaagaaat
    17641 ggacgaatta gaccaacaat ttccgcaata cggcttcgca cagcacaaag gctacccgac
    17701 taaactccat tttgaaaaat tagcagaatt tggggcaaca ccttatcatc gtaagagttt
    17761 tgcccctgta agaaaagtat taggtttaat cgattagaag gaaaacacaa tgactacaga
    17821 gtaccaagaa acgattttca ccaaaatcat caataaagaa attccggcaa atattgtcta
    17881 tcaagatgaa cttgtgaccg catttcgtga tattgctcca caagcaccca cgcatatttt
    17941 gattattccg aataaattta ttccaaccgt caatcacgtg agtaatgaag atgaattagc
    18001 cctagggcgt ttatttacgg tggcggcaaa attagcc
//
LOCUS       DANKBV010000039        17879 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.039, whole genome shotgun sequence.
ACCESSION   DANKBV010000039 DANKBV010000000
VERSION     DANKBV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17879
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.039"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..487
                     /locus_tag="PWY43_001074"
     CDS             <1..487
                     /locus_tag="PWY43_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL2135964.1"
                     /translation="GGTLPMKEVGSFNVLAEFVGKVDLPIMLAGGIADKKAVQAALTL
                     GAEGVWIGTAFIATEECRASEKVKNWIVEANANDLLLFRTEPHYYRSLPTELAKKCFE
                     MSENGATRSEIAKVMNASTGMRLGMLEGDFENGYVSVGNGISAIDRIKTVKELIDELM
                     A"
     gene            complement(550..894)
                     /locus_tag="PWY43_001075"
     CDS             complement(550..894)
                     /locus_tag="PWY43_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2135965.1"
                     /translation="MNKYALLAIFWFIIGIYALLFRESTNDIPPFPHFDKVSHFALFF
                     IQIWLSANAFIRSKKGIPYFGLIIFALLYAFTSEWAQATFTSTREGSWLDGLADMLGA
                     SLALLLVKYCKK"
     gene            complement(995..1264)
                     /gene="yedF"
                     /locus_tag="PWY43_001076"
     CDS             complement(995..1264)
                     /gene="yedF"
                     /locus_tag="PWY43_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDL2135966.1"
                     /translation="MAFTVVQKLDKDPKDITPDYTLDTLGEPCPYPAITMLETMPQLQ
                     KGEILELLSDCAQSINNIPIDTKNHGYTLLSVEQDGTKLRYLIQR"
     gene            complement(1305..2507)
                     /gene="yedE"
                     /locus_tag="PWY43_001077"
     CDS             complement(1305..2507)
                     /gene="yedE"
                     /locus_tag="PWY43_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDL2135967.1"
                     /translation="MLAIWQSFKQNYLIKYWNPVAAVIAAGIISAYYFGITGTYWAVT
                     GEFTRWGGHLLNALGVDVSNWSYFQLIGLDGTIFTRIDGVMILGMFAGCLSAALWANN
                     VKWRNQPHKKRIIQALIGGALAGFGARLAMGCNLASLFTGIPQFSVHAWFFTFATAIG
                     TYFGVKVTLLPMFRVKLELKKGAAKIKENDPKQANRRFWVGMLIFITYLSASLYVMQS
                     SIKLGFAMLCGLAFGLLIERAQICFTSAFRDLWITGRAYMAKAIIFGIIAGTIGVFSY
                     IQLGVSPKILWAGPNAIIGGLLFGFGIVLAGGCETGWMYRSMEGQVHFMWVGVGNVVG
                     STLLAYYWDDLAPVLTLDYEKINLLKEFGPVGGLFVNYGLLILCLIAVVWWEKRFLAK
                     AKAKLATA"
     gene            complement(2731..4326)
                     /locus_tag="PWY43_001078"
     CDS             complement(2731..4326)
                     /locus_tag="PWY43_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2135968.1"
                     /translation="MQNEKQLIEQGNTVIGIELGSTRIKAVLISSDGTILATGGADWE
                     NRLIDGIWTYHQNEIWEKLQAAYMDLSKTVKEKYHTTMRTAKALGISAMMHGYLPFDK
                     HGNQLVAFRTWRNNITLTSSEKLTALFQYPIPQRWSIAHLHQALLNQEPHLPEIDYLT
                     TLSGYVHWQLTDHKVLGIGDASGMFPIDTELKSYNPTMLAQFDRAIADYRMPWKLEAI
                     LPQVRVAGEQAGKLTEKGAKLLDPTDNLQAGIPLCPPEGDAGTGMIATNCIKEKTGNI
                     SAGTSAFAMIVLEKALSKAYSELDIVTTPAGKLVAMAHANNCTSDINAWVNLFGECLS
                     VFGVNICTEELYEMLFLHALQGDADCGGLLSYGFHSGEHNVGLSEGCPVFLHPTNAHF
                     NLANFMRTHLYTAFGAMKLGMDILINQEKVEISRILGHGGIFKTEGVAQKILSSALNI
                     PLATASTASNGGAWGIALLADFLSVSDRYRLEEYLDNCIFNTTKLNLVQPDKTMSEGY
                     ERFMQRYKQGIPVVEKALFLNTD"
     gene            complement(4339..5289)
                     /locus_tag="PWY43_001079"
     CDS             complement(4339..5289)
                     /locus_tag="PWY43_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2135969.1"
                     /translation="MKSKLDPTIFYLLALFAVAVIAFSIFIPDIFWSVSNFQSMASQI
                     PVLGILTLAMALPMLTGGINLSIIAGMNACSLIIAYFITQYTGISWLFIAILLSLCCA
                     LAIGLLNGTLIAIIRVSPILATLGTMTLINGINILVTNGSTIANFPETMLSINASTFL
                     WIPLPILIFLFLAGLIWFFLEKTAMGKAVYFIGNNEKATYYSGINTKKVLIWVYVISS
                     ILCVFAAILMMSKLNSAKASYGDSYLLISILAAVLGGINPDGGKGKLLGIMLALVLLQ
                     VIESGLNMLGISSYITMILWGSLLLFFIFLQKNRLFRLTQ"
     gene            complement(5292..6251)
                     /locus_tag="PWY43_001080"
     CDS             complement(5292..6251)
                     /locus_tag="PWY43_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2135970.1"
                     /translation="MKISNNHILSSTLLILILLLTLFTRDFATIENLYDVVNNYAMLM
                     ILACGLFVVLLSGGIDISFPAITIISQYVMVTLIGKYDVGFAGVFVISSTIGILLGFI
                     NALFVNRLSVPSIIITISTLNIFYGGLLYFTKGVWLYEYPEWFTQEIVFFKQITAEGV
                     EFGLSFQALMAILAVILTGLITNKLTIGRKIYALGGNPDATSRIGFNLLFLHLFAYGY
                     MGFMAGLAGIVQSYTVQSVAPDSLLGYELTVLAAVVLGGASLSGGRGTLLGTVMGVML
                     LAIIQNGLNLMNVSSYWQTIITGIIIILSISATALSQRKKQGA"
     gene            complement(6235..7725)
                     /locus_tag="PWY43_001081"
     CDS             complement(6235..7725)
                     /locus_tag="PWY43_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2135971.1"
                     /translation="MAFISMRHISKTFHGIKALNQVNLSLDYGEALCLAGQNGCGKST
                     LIKILSGVYQPDKGAEIQIGATQYTKLTPQESIDKGIQVIYQDLALFPNLTVAENIAI
                     NLHRKFGWVSQDHIHQVALTAIQSINANLDLNAILEDLPIAQQQLVAICRALAQNARL
                     LIMDEPTASLTAKEVKDLLDVVIKLKSKGISIIFVSHKLQEVMSVSDSVLVLKDGNMV
                     GQYPIREMNEKRLGFLMTGLEIDYKRLDLPDFLQRKTVLEVRNLTLPHQYQEINFSLR
                     EGEIVALTGLLGSGRTELCLSLFGITQPQSGDILLNGEKVIFNTNRDAIAKGIAYVSE
                     DRMHTGLIMAESIHHNIISTIFHKITDKLQIIQSSKAYASSQALIDSLKIKVSDSDLP
                     VNTLSGGNAQRVSIAKWLAIDPKIIILDAPTIGVDIANKEGIFQIIKSLAQQGIAVIF
                     VTDEVEETYYNSHRVIVMKKGRIVDEILPAYSSEKSIAEVIYENQQ"
     gene            complement(7782..8756)
                     /locus_tag="PWY43_001082"
     CDS             complement(7782..8756)
                     /locus_tag="PWY43_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2135972.1"
                     /translation="MKKPTFKHALFSSILALACYGSAFANDVVNISKIDGMPWFNRMA
                     EGVVQAGKDNNINAYQVGPSNTDAPQQVKLIEDLIAKKVSAISIVPNDANALEPVLKK
                     AKQNGIVVLTNESVGQPSADWDIEIIDNAQFAADYVEEVAKAMGGKGGYVIYVGGLTV
                     PQHNLWADLFVKYQKEHYPDMFEVTSRMPVAENINDARRTTIDLVKAHSDLKAVVSFG
                     SQGPIGAGLAVKEKRLKNKLSVFGMMIPSQAANLIKSGDITMGITYDPANAGYALAAV
                     AAKVLKGEKIEDGLEIPTVGKAKVDSEKKLLQFHNVLKVTKENIDKLY"
     gene            complement(8943..9803)
                     /gene="araC"
                     /locus_tag="PWY43_001083"
     CDS             complement(8943..9803)
                     /gene="araC"
                     /locus_tag="PWY43_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDL2135973.1"
                     /translation="MYKNLSLLSPTYPFDLEFIAGLTQCEKGNYLDSIVDRQQGMQGY
                     MLQLTTFGHGSVSDGKHVFSAHRGQLLLFTPNAVQHYHRHPESQYWHYKWVYFLPNPK
                     WNKWLNWSNMEEKIGRITISDDRYFQEISQLFSKIELELKSSKFFKEDIATSLLEYLL
                     MKCISAEKIEVIPVIDQRILTVCDLILTNLAKNESLEAMAEKVFLSPSRLSHLFKQSL
                     GISLVQWRELQRISESKKLLYFSNISISNIAKSLGYEDSLYFSKIFKKHTGLSPSEFR
                     TLERRKDSEM"
     gene            complement(9918..10832)
                     /locus_tag="PWY43_001084"
     CDS             complement(9918..10832)
                     /locus_tag="PWY43_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL2135974.1"
                     /translation="MLSPYQLVLINLLYIFGIGLNIVLLRFLSLHFDPLNNNGVRFLI
                     GGLTLLVFIFWRFRTSLSQLIKTPKLIAISLFVGLMMSANMYFWLKGVKITNAVTASI
                     FGVLAMPFGVIIAAIFFQDERERIKSKAFWFGSILTIIGSLGFIWHGKTLSIGEGFIL
                     GSMFLFLSIIIRNIQNLVVKFARELNVFTLSCVTSLTTSLSSLLLSQQAGKFNELFTA
                     PNWLLFSLMAAGIYAIMIGMVLTFHIIQTQGLITYQVLELLMPVSTAIIAYILLDERI
                     SIVQMGFACIVILGSSIALKLVKYPKHY"
     gene            complement(10923..11141)
                     /gene="cspD"
                     /locus_tag="PWY43_001085"
     CDS             complement(10923..11141)
                     /gene="cspD"
                     /locus_tag="PWY43_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HDL2135975.1"
                     /translation="MDIGIVKWFNNVKGFGFITSDVCEGDIFAHFSEIQAEGYRSLKV
                     GQKVQFELVQGERGASASNITPISEKTL"
     gene            complement(11243..11815)
                     /locus_tag="PWY43_001086"
     CDS             complement(11243..11815)
                     /locus_tag="PWY43_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HDL2135976.1"
                     /translation="MKILRKFAAVCVVSMAMLSANGNLLSISEQEINDYLATKLAEKI
                     PLQDKVGVPHLFELDYHLHSLTSQIGRTEEKKVAISGVVDGLLKAKGKKYDASIQLNM
                     DTTPYYDPQKGALFLKDVRLLSWSATPAKYQAELQMFLPVLADGLNSFLNSTPVYTLD
                     ENKAKEAMVKKFGKAIVIENGNIKLETTIF"
     gene            11909..12049
                     /locus_tag="PWY43_001087"
     CDS             11909..12049
                     /locus_tag="PWY43_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135977.1"
                     /translation="MKKLLLCVGILVLAACSSTGVGGSIGAGGGSNGVGIGLGVGTDF
                     RF"
     gene            12108..13262
                     /gene="tgt"
                     /locus_tag="PWY43_001088"
     CDS             12108..13262
                     /gene="tgt"
                     /locus_tag="PWY43_001088"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL2135978.1"
                     /translation="MKYELKTTSGNARRGRLTFNRPKGEFHVETPAFMPVGTYGTVKG
                     MTPEEVKATGAQILLGNTFHLWLRPGQEVMKMHGDLHDFMQWHGPILTDSGGFQVFSL
                     GKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
                     KSMEMSLRWAKRSRDRFDELENPNALFGIIQGGIYEELRKVSVEKLVEIGFDGYAVGG
                     LAVGEPKEDMHRILEYVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNA
                     RNGHLFVTNGIVKIRNAKYRNDITPLDPECDCYTCKNYTKAYLYHLDKCGEILGARLN
                     TIHNLRYYQRLMAEIRQAIEEDRFDDFVVEFYARIGKEVPPLQSEVNGQK"
     gene            13275..13786
                     /locus_tag="PWY43_001089"
                     /pseudo
     CDS             13275..13786
                     /locus_tag="PWY43_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
     gene            13834..14046
                     /locus_tag="PWY43_001090"
     CDS             13834..14046
                     /locus_tag="PWY43_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL2135979.1"
                     /translation="MNYSLDLTDYACPLPLLMAKKAMNDLKKGDSLEILLNQQSSLTD
                     FELLAKEQGWLCELQAVENRVIFTIA"
     gene            complement(14043..15407)
                     /locus_tag="PWY43_001091"
     CDS             complement(14043..15407)
                     /locus_tag="PWY43_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3327 domain-containing protein"
                     /protein_id="HDL2135980.1"
                     /translation="MFKSELSGTATFSILANGATVSDQDFTLTIERHIPISAQIAPPK
                     VIENTQLEELSQKIAKNPEKQTALINDFWDKAKQAGTPLIEPLDEQQSRVTFLWRGAT
                     KNVRIWGGVSTEHDFMQRFGETDIWYKSYIVPNDTLVEYKLAPDVPTLPVDENTQRRA
                     LLATAQADPLNKTAYFEKIGQNIHNTDKFNYASLLKLPNAPTQSFLTEAPNIAKGKLQ
                     QLIFESATLGNKRRLFVYLPDGFSAEKNYAVLYLFDGVEYTERVPTKTILDNMIAQKV
                     ILPTVAVFIENPSSESRRVELPANPIFTQVLAKELVPFVEKQLNIKTKERIIGGSSYG
                     GLSSAYTVLNYPNVFSKALILSGSFWRQPKGTPSEQSHYIAQQFIQKEKLPLCFFISA
                     GFYESGKSAILETSRHLKDVLLAKGYPVTYVEQSTSHGYLAWQGLISEGLKTLLGSNK
                     CTSN"
     gene            complement(15389..15610)
                     /locus_tag="PWY43_001092"
     CDS             complement(15389..15610)
                     /locus_tag="PWY43_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HDL2135981.1"
                     /translation="MKKTTACILTFLVSSVLYANNITIELKQNDFVQSKLIQKAQQPL
                     SLSIIFPAKALACWLKMSLVNKILCLNQN"
     gene            complement(15654..17729)
                     /locus_tag="PWY43_001093"
     CDS             complement(15654..17729)
                     /locus_tag="PWY43_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL2135982.1"
                     /translation="MKIKKYSLSLITLAIVAPQVLAETTSQAELNAIEVKEKLAATTG
                     YIPVYTTVGTKTLTSLARTPFSINVVSDDLMHDQAVRSVTDSVAYTSGMMGGYRGNNS
                     LIEISIRGIGNKSDGSTIPTYLNGSRYQTAFEIDPFFLESINVIKGPNSILYGQANPG
                     GVMDIITKKAGSNPHRHLQFVVGNHQRYQIGLDIEENLSNTLSARLIGQLERTNWNTQ
                     FVKEKGGAFAPSILWKPTTNTELNVYAYYAKKPKAGDRNFLPKEGTINSVNGKKLPYD
                     MFLSDLNYHGLKAEQMQVGYNFSHKFTPNLTFRQNASYHNGEENFKNLVYWGDVGSVL
                     ERRARRWDVKTQEINLDNQLEWVFSTANINHTLLAGIDYKRVREDLDNYMGIAPDFDW
                     QNPVYGVTVTEPSLFSSEVKHLKQLGVYLQDQIEIGNFDFLLGGRYDRAKNSNQDRLN
                     SKTENNQEGKFTWRTGVVYNFENGIAPYISYSTSFVPSAEKDAQGNFLKPTTAGQWEV
                     GMKYQPTQDTLLTLAGFKITQKDLANYQWQTRSYEQIGEVQTKGIEVSLNQQLSDKFS
                     IAPSYAYLKKEITADKDGTTIGKTQWGVPRHQASLWAKYKFFDKLTAGVGVRYMGTTF
                     GDNQNTFTVPAYTLYDMMLAYDFMPNLNLQLNAQNLTNKKYVASCANNFSCFYGKDRN
                     LSATLNYHF"
BASE COUNT         5488 a         3447 c         3592 g         5352 t
ORIGIN      
        1 gggcggtact ttgccgatga aagaggtagg cagttttaat gtgctggcgg aatttgtggg
       61 caaggtggat ttgccgataa tgctggctgg tggtattgcc gataaaaaag cggtgcaggc
      121 tgccttaacg cttggggctg aaggcgtgtg gattggcacg gcatttatcg ccaccgaaga
      181 atgccgtgca agcgaaaaag tgaaaaactg gattgtggag gcaaatgcca atgatctgct
      241 gttattccgt actgaaccac attactaccg ttcactgccg actgaattag cgaaaaaatg
      301 ctttgaaatg agtgaaaatg gggcaactcg ttctgaaatc gccaaagtga tgaacgctag
      361 cacaggaatg cgtttgggta tgctggaagg cgattttgaa aacggctatg tgtcggtggg
      421 caatggcatt tcagcgattg accgcattaa aacagtgaaa gaattgattg atgagttgat
      481 ggcataattt tggtaaaaaa tgaccgcttg tagtatggct aaaccatata acaagtggtc
      541 ttttatgctt tattttttgc aatattttac tagcaataat gcaagtgatg cacctagcat
      601 atctgccagt ccatcaagcc aagatccttc tctggttgag gtgaaggttg cctgagccca
      661 ttcgctggtg aaagcataga gtaaggcaaa aattattaac ccgaaataag gtatcccttt
      721 ctttgagcgg ataaaggcgt ttgcagataa ccaaatctgg ataaagaata aggcaaaatg
      781 gcttacttta tcaaaatgtg gaaaaggtgg tatatcattg gtgctttcac gaaaaagtaa
      841 agcataaatg ccaataataa accaaaatat tgcaagtaaa gcgtatttat tcatttttct
      901 tttacctctt agaataaaac gagccttgcg ttacaaggct cttgaatagt tgctatttta
      961 cgacaagcgg tcgtttttca ctaatgtttt gcaattaacg ctgaattaaa tagcgtaatt
     1021 tagtgccgtc ttgttccacg cttaataggg tatagccgtg atttttagta tcaatcggaa
     1081 tattattaat cgattgggca caatcgctta acagttccaa tatctcgcct ttttgtagtt
     1141 gaggcattgt ttctaacatc gtaatcgcag ggtaagggca aggttcgcct aaggtatcta
     1201 aggtgtaatc aggcgtgatg tctttcggat ctttatctag tttttgcaca acggtgaatg
     1261 ccatagtcat tttcctcttg ttggttgaaa ataaattaaa aagattacgc agttgccaat
     1321 tttgccttcg ctttggcaag gaagcgtttt tcccaccaaa ccacagcaat taagcaaaga
     1381 attaataagc cataattcac gaataatccg ccaaccggcc caaattcttt gagtaggttg
     1441 attttttcat aatcaagggt aagcaccgga gctaagtcgt cccagtagta agccagcagg
     1501 gttgaaccga ctacattgcc tacgccgacc cacataaaat gaacctgtcc ttccattgaa
     1561 cggtacatcc agccggtttc acagccgccg gcaagcacaa tgccaaatcc gaataataag
     1621 ccgccgataa tggcatttgg gccagcccac aaaattttag gtgaaacacc taactgaata
     1681 taagagaaca cgccgattgt gccggcaata atgccgaaaa taattgcttt tgccatataa
     1741 gcccgtccgg taatccataa gtcacggaaa gcggaggtaa agcaaatttg ggctcgttca
     1801 atcaataagc caaaggctaa gccgcaaagc attgcaaaac caagtttgat tgaggattgc
     1861 attacataaa gggaagcaga aagataagtg ataaaaatga gcataccaac ccaaaaacgg
     1921 cgattagctt gtttcggatc gttttctttg atttttgctg cccctttttt cagctctaat
     1981 ttcactctaa acatcggtaa taaagtcact tttaccccaa agtaagtgcc gatggcggtc
     2041 gcaaaggtga aaaaccaagc gtggacggag aattgcggaa taccggtaaa aagagaggcg
     2101 aggttacagc ccattgctaa acgggctccg aaaccggcta acgcaccgcc aatcaaggct
     2161 tggataatcc gttttttatg tggctgatta cgccatttga cgttgttcgc ccataatgca
     2221 gcagaaagac agccggcaaa cataccgaga atcatcacac cgtcaatgcg ggtaaagatg
     2281 gtgccgtcta agccgatgag ttgaaaatag ctccagttcg aaacatcaac acccaaagca
     2341 tttaacagat gaccgcccca acgggtaaat tcccctgtaa ccgcccaata agtgccagta
     2401 atgccgaaat aataagctga aataatacca gctgcgatca ctgctgcaac aggattccaa
     2461 tatttgatta gataattttg tttaaaactt tgccaaatcg ctaacataaa acaccttaac
     2521 taaggataga ttaatcggat ttgcaagagg gtataacgat gaagaaaggg actgattgat
     2581 acagtaatta gttcatttcc tatacacaaa acttgcgata tatcaatgca cattttacaa
     2641 atgcgtcttt atagtaggta ttttttgtac aaatgtaaat aacaagcggt cgtttttggc
     2701 aaaaataacc gcttgtgata cttaaattat ttaatctgta tttaaaaaca gcgctttttc
     2761 taccacagga atgccttgct tataacgttg cataaagcgt tcataacctt cactcattgt
     2821 tttatccggt tgaaccagat tgagtttggt ggtgttgaaa atgcaattat ctaaatattc
     2881 ttctaaacgg taacggtctg atacgctgag aaagtctgcc agtaatgcaa ttccccaagc
     2941 tccgccattt gaagcagtac ttgcggtagc caatggaata ttcagtgcgg aagacagaat
     3001 tttttgtgca acaccttctg ttttaaatat accaccgtga ccgagaatac gggaaatttc
     3061 cactttttct tggttaatga gaatgtccat tcctaatttc attgcaccaa aagcggtata
     3121 gagatgggtt cgcataaagt ttgctaagtt aaaatgggca ttcgttgggt gcaagaaaac
     3181 cggacagcct tcggataaac cgacattatg ttcaccggag tggaagccgt aggataatag
     3241 ccctccacaa tctgcatcgc cttgcaaggc atggagaaat aacatttcat aaagttcttc
     3301 ggtgcagata tttacgccaa aaaccgataa gcattcgccg aataaattca cccacgcatt
     3361 aatatcagag gtgcaattat tagcgtgagc cattgccact aatttacctg ccggggtcgt
     3421 cacaatatcc agctctgaat aggctttgga tagggctttt tctaacacaa tcattgcaaa
     3481 tgcggaggtg ccggcagaaa tattaccggt tttttctttg atacagttgg ttgcaatcat
     3541 acctgttccg gcatcaccct caggtggaca aagtggaatg ccagcttgta aattatcggt
     3601 tggatctaac aattttgcac ctttttcggt taatttgccc gcttgttcac cagcaacacg
     3661 aacttgtggc aaaatcgctt ccaatttcca aggcatacgg tagtcggcaa tggctcgatc
     3721 aaattgagcc agcattgttg gattgtagga tttcaactca gtatctatcg ggaacatacc
     3781 ggaagcatcg ccaatcccta acactttgtg atcggtcagt tgccaatgca cataacctga
     3841 gagggttgtc aggtaatcaa tttccggcaa gtgaggctct tggttaagca atgcttggtg
     3901 taaatgagca atactccaac gttgcggaat tgggtattga aagagtgcgg tcaatttttc
     3961 cgatgaggtt agggtaatgt tgtttcgcca agttctaaag gcaaccagct gatttccgtg
     4021 tttatcgaaa ggtaagtagc cgtgcatcat cgcactaatt cccaatgctt tggctgtgcg
     4081 catggttgtg tgatattttt ctttgactgt cttgcttaaa tccatataag cagcttgtag
     4141 tttttcccat atttcatttt gatgataggt ccaaataccg tcaatcagtc gattttccca
     4201 atccgcccca ccggttgcta aaatcgtccc atctgagcta attaacaccg ctttaattcg
     4261 ggttgagccc aattcaatac caataacagt attaccttgt tcaatgagtt gtttttcatt
     4321 ttgcataatc taccaccatt actgtgttag tcggaaaagg cgattttttt ggaggaagat
     4381 aaagaataac aataagcttc cccataaaat cattgtaatg taactgctaa tgccgagcat
     4441 atttaagcca ctttcaatca cttgtaataa aactaaggcg agcataatcc ctaatagttt
     4501 ccctttacct ccatcagggt taataccgcc caatactgcg gctaaaatgg agatcaacaa
     4561 ataggaatca ccataagagg cttttgcaga attgagttta gacatcatca aaatagctgc
     4621 aaatacacaa agaattgagg agataacata aacccaaatc agcacttttt tggtattaat
     4681 acccgaataa taggtggctt tttcattatt gccgatgaaa taaaccgctt tacccattgc
     4741 agttttttct aagaaaaacc aaatcagccc agcaagaaag agaaagatta gaatgggtag
     4801 cggaatccat aaaaaggttg aagcattaat actcaacatt gtttccggaa agttcgcaat
     4861 ggttgaaccg ttagtgacta gaatattaat gccgttaatt aaagtcattg tgcctaaggt
     4921 tgccaaaatt ggtgaaacac gaataatggc aattaatgtg ccgttgagta atccaatggc
     4981 taaggcacag caaaggctta ataaaatagc aataaataac caagaaatac cggtatattg
     5041 cgtaataaag taggcaataa ttaatgagca agcattcatt ccggcaataa tggaaagatt
     5101 tattccacct gttaacatcg gtaatgccat tgctagggtt aaaataccta atactggaat
     5161 ttgtgatgcc atagattgaa agttactgac agaccaaaaa atatccggaa tgaaaatact
     5221 aaaggcgata actgcaactg cgaagagtgc taagagatag aaaatcgttg gatccagttt
     5281 acttttcatc gttatgctcc ttgttttttc cgctgactta aggctgttgc actaatactt
     5341 aagataatga tgataccggt aataatggtt tgccagtaag acgatacatt cattaaattt
     5401 agcccgtttt gaataatcgc cagtaacatt acgcccatta ctgtaccaag cagggtgcct
     5461 ctaccacctg ataaactagc accacctaat acaacggcag ctaggacggt taattcataa
     5521 cccagtagtg aatccggtgc gacagattgt acggtataag attgcactat gcctgccaag
     5581 cctgccataa agcccatata accgtatgca aatagatgga gaaataagag attaaaccca
     5641 atacgggagg ttgcatccgg attaccacca agagcgtaaa tttttcgacc aatagtgagt
     5701 ttattggtaa ttaagccggt taatatcacc gccaaaatag ccattaatgc ttgaaacgat
     5761 aatccaaatt ccactccctc agcagttatc tgtttaaaga acacgatttc ttgggtaaac
     5821 cattcgggat actcataaag ccatacacct ttggtaaaat agagtagtcc gccataaaaa
     5881 atattgagtg ttgagatcgt aataataatg gaagggacac taagccgatt aacaaataga
     5941 gcattaataa aacctaataa tatgccgatg gtggaagaaa taacaaaaac gccggcaaat
     6001 cctacatcat atttaccgat cagagtgacc ataacatatt gggaaataat agtgatagca
     6061 gggaaagaaa tatcaatgcc gccagacagc aataccacaa ataaaccaca agctaaaatc
     6121 attaacattg cgtaattatt tacaacatca taaaggttct caatagttgc aaaatcacgg
     6181 gtaaataggg ttaataatag gattaaaatc agtaacgtac tggaaagaat atgattattg
     6241 ctgattttca taaataacct ccgcaatcga tttttcactg gaatatgccg gcaagatttc
     6301 atcaacaatg cgaccttttt tcattacaat tacccgatgg ctgttgtagt aggtctcttc
     6361 cacttcatca gtcacaaaaa taactgcaat gccttgctga gccaatgatt taataatttg
     6421 gaagatccct tctttattgg cgatatcgac cccaatagtc ggggcatcta aaataataat
     6481 tttggggtcg attgccagcc attttgcaat cgaaactcgc tgtgcgttcc ctccggaaag
     6541 ggtattaaca ggcaaatctg aatcggatac tttaatcttt aatgaatcaa tcaaggcttg
     6601 gctacttgca taagccttcg aggattgaat aatctgtaat ttatcggtga ttttgtgaaa
     6661 aatagtcgaa ataatattgt gatgaatcga ctctgccata atcagcccgg tgtgcattct
     6721 atcttccgat acataagcaa tccctttggc aatggcatca cggttagtgt taaatatcac
     6781 tttttcaccg tttaataaaa tatcgccgga ttgtggttga gtaatgccga ataaacttag
     6841 gcaaagctca gtacgccctg aaccgagcaa tcctgtaagg gcaacaattt ccccttctcg
     6901 tagcgaaaag ttaatctctt gatactgatg aggcagcgtt aaattgcgga cttccaatac
     6961 ggtttttctt tgcaaaaaat ccggcaaatc taaccgcttg taatcgattt ccagcccagt
     7021 cattaggaaa ccaaggcgtt tttcattcat ttcacggata ggatattgcc caaccatatt
     7081 gccatctttc agcactaata cgctatcgga aacactcatt acttcttgca atttatgact
     7141 gacaaagatg atgctaatgc ctttgctttt aagtttgatg acgacatcta acagatcttt
     7201 gacctctttt gcagtaagtg aggcggtcgg ctcgtccata atcaataatc tggcattttg
     7261 tgctaacgct cgacaaatag cgactaactg ttgttgagcg atcggtaaat cttctaatat
     7321 tgcattcaga tctagatttg cattaatgct ttgaatagca gtgagggcaa cctgatgaat
     7381 gtgatcttga ctcacccagc caaattttcg gtgcaggtta atggcaatat tttctgccac
     7441 ggttaaattg gggaagaggg ctaaatcttg ataaatcact tgaatgcctt tatcaatcga
     7501 ttcctgtggt gtcagcttgg tatattgggt tgcaccaatt tgaatttcag cccctttatc
     7561 cggttgataa acgccggaaa gaattttgat caaggttgat tttccacaac cattttgacc
     7621 ggcaaggcaa agtgcttcac cataatctaa tgagagattg acttgattta atgcttttat
     7681 gccgtggaat gttttactga tatgccgcat cgaaataaat gccataagtg cctcctgagg
     7741 ggaaaatatg ggctggttat cagcccatat gtcagcgcaa attaatataa tttatcgata
     7801 ttttccttgg tgactttcag tacattgtgg aattgaagta gttttttctc actatctact
     7861 ttggctttac caaccgttgg gatctctaat ccgtcctcaa ttttttcgcc ttttaatact
     7921 ttggcagcga cagcagctaa agcataacct gcatttgccg ggtcgtaggt aatgcccatc
     7981 gtaatatcac cactcttaat taaattggct gcttgggaag gaatcatcat tccgaagaca
     8041 cttaatttat tttttaagcg tttttctttc actgccaacc ctgcaccaat aggaccttgt
     8101 gagccaaaag aaacgaccgc tttaagatcg gaatgggctt tcactaaatc aatggtggta
     8161 cggcgtgcat cattaatatt ttcggcaaca ggcattctgc tggtcacttc aaacatatct
     8221 ggataatgct ctttctggta tttcacgaat aaatcagccc acagattatg ttgaggcacg
     8281 gttaaaccac caacataaat cacatagccg ccttttccgc ccatagcttt tgctacttct
     8341 tctacatagt ctgccgcaaa ttgtgcatta tcgataattt caatatccca atcggcacta
     8401 ggttgtccta ctgactcatt ggtcaatacc acaatgccgt tctgtttcgc ttttttcagt
     8461 accggttcta aggcatttgc atcgttaggg acgatggaaa tagcactaac ttttttcgca
     8521 attaaatctt caattaactt tacttgttgt ggagcatcgg tatttgatgg accaacttga
     8581 taagcattga tgttgttatc tttacctgct tgcacaacac cctctgccat acgattaaac
     8641 cacggcatac cgtcaatttt ggagatatta acaacatcat tggcaaatgc cgaaccgtaa
     8701 caggctaaag ctaaaatact tgaaaataat gcgtgcttga acgttggttt tttcattgtt
     8761 cttccctcat acataagaag ttatttcact gaatatcact cacagagctt gatatccctt
     8821 ctctaataga aacagcacta ttaaagctgt ttgtatctta tgcttttgag tgagagaaaa
     8881 acaggtaact ttttgttatc gatatggaaa attctgctat gcaaagtaaa gtgagatcaa
     8941 gatcacattt cactgtcttt acgccgttct aaggttctaa attcagaggg tgacagcccg
     9001 gtatgctttt tgaaaatttt ggaaaagtag agagaatcct catagcctaa ggatttcgca
     9061 atattagaga tagagatgtt agagaaataa agcaattttt tggattcgga aatgcgttgt
     9121 aattcccgcc attgcactaa gctgatcccc agcgattgct tgaaaagatg tgagaggcga
     9181 gatggcgata aaaatacttt ttctgccatt gcttctaagc tctcattttt agccaaattc
     9241 gttaagatta aatcacaaac tgtcaaaatg cgttgatcaa tgacaggaat aacctcaatt
     9301 ttctcggcgg aaatacattt cataagtaag tattccaaca aacttgttgc aatatcttct
     9361 ttaaaaaatt tactggattt aagttctaat tcaattttac tgaaaagttg gctgatttcc
     9421 tgaaagtaac ggtcatcgct aatggtgatc cgccctattt tttcttccat atttgaccaa
     9481 ttcagccatt tattccactt tggattaggc agaaaataga cccatttata gtgccaatat
     9541 tggctttctg ggtgacgatg gtagtgctga actgcattgg gtgtaaatag gagtaattga
     9601 cctcgatggg ctgaaaagac gtgtttacca tcagaaacag agccatgccc aaaagtagtt
     9661 aattgcaaca tataaccttg catcccttgt tgcctatcga caatagaatc aagatagttg
     9721 cctttctcac attgagtaag accggcgatg aattcaaggt caaatggata agtcggtgaa
     9781 agcaaagaaa ggtttttata catgactatc agtatttacc aaaaaataat aaaaaataac
     9841 cgcttgttta tcattactaa tgaacgccca gattagtaaa ccaaatgtaa ttttaccaat
     9901 attaattagc aaagatccta atagtgctta ggatatttta ctaactttaa ggcgatactt
     9961 gagcctaaaa tcacaataca agcaaagccc atttgaacaa tggaaatacg ttcgtccagt
    10021 aaaatataag caatgatggc tgttgaaacc ggcattaata gttccaatac ttggtaggtt
    10081 atcagtcctt gggtttggat aatgtgaaag gttaatacca ttccgatcat aatcgcataa
    10141 ataccagcag ccatcagaga gaatagtagc cagtttggtg cagtaaaaag ctcattaaat
    10201 ttacctgctt gttggctaag taatagactt gagagtgaag tagtcaaaga tgttacacaa
    10261 cttagtgtaa atacgttcag ctctcgagcg aattttacaa ctaggttttg tatgttacgg
    10321 ataataattg acagaaataa gaacatagaa ccaagaataa atccttcgcc aatcgagagt
    10381 gttttcccgt gccaaataaa tccaagtgaa ccgatgatcg ttaaaattga gccgaaccag
    10441 aaagctttgc ttttaatgcg ttctctttca tcttgaaaga atatggcggc aataatcacg
    10501 ccaaatggca ttgccaatac cccaaaaata gaggcggtaa cagcatttgt aattttgacc
    10561 ccttttagcc aaaaatacat atttgcggac atcattaacc cgacaaatag agaaatcgca
    10621 atcaattttg gagttttaat cagctgagac agcgatgtgc ggaatctcca gaaaataaaa
    10681 accaataaag tgagtccacc aattaggaag cgtacaccgt tattatttaa tggatcaaaa
    10741 tgcaaactga gaaacctaag cagtacaata tttagaccaa taccgaagat ataaagtagg
    10801 ttaattaaga ctaattgata aggggaaagc atattcaaaa aaacaaaaat ggctcagctg
    10861 taaaagctga gctttaaaat ctaataaagt atggttataa caataatttt attaacgatg
    10921 ggttataacg ttttttcgct tataggcgtg atatttgagg ctgaggcacc tcgctctcct
    10981 tgaactaatt caaactgtac tttttggccg acttttaaag agcgataacc ttctgcttga
    11041 atttcagaaa aatgagcaaa aatatctcct tcacatacat ccgatgtaat aaaaccaaat
    11101 ccttttacat tgttaaacca tttaacaata ccgatatcca tagaactctc caaataatga
    11161 atgacgatat ttggattgta aataaaacgc gtaaaagctc aactatcaaa gtgtaaaaat
    11221 tagacgaaga tcacaaaatt atttaaaaaa tagtagtttc tagcttgata ttaccatttt
    11281 ctatcacaat ggctttgccg aactttttca ccattgcttc ttttgcttta ttctcatcta
    11341 aagtataaac aggcgtgctg tttaaaaaac tatttaatcc atcagccaat accggcaaaa
    11401 acatttgcag ttctgcttga tatttagccg gtgtagcaga ccagcttaac aatctgacat
    11461 ctttaagaaa taatgcccct ttttggggat cgtaataagg cgtggtatcc atatttaact
    11521 ggatagacgc atcatatttc ttgcctttcg cttttaaaag cccatcaaca acaccggaaa
    11581 tagccacttt tttctcttcg gttctaccaa tttggcttgt cagcgaatgc agatggtaat
    11641 ccaattcaaa taaatgcgga acgcccactt tatcctgtaa agggattttt tcagctaatt
    11701 tggtggctaa atagtcgttg atttcttgct cgctaatgct gagtaaatta ccgttagcag
    11761 agagcatagc cattgagaca acgcatacag cagcgaattt acgtaaaatt ttcataattt
    11821 accctttaaa attaggttta atccattaaa caggttagaa tacattgggt ctatcataga
    11881 ctctcttttt cgtttaggac agcaaaaaat gaaaaaactc ttattatgcg ttggcatttt
    11941 agtgttagct gcttgctcat caaccggtgt gggtggcagt attggagccg gtggtggctc
    12001 taacggcgtt ggaatcggct taggtgtcgg caccgacttt cgattctaat tggtaaattt
    12061 ttggcggttt ttgaccgctt tcacgaactg tttatttgtg gattaaaatg aagtacgaat
    12121 taaaaacaac aagtggtaac gctcgtcgtg ggcgtttaac ttttaaccgc ccgaaaggcg
    12181 aatttcacgt tgaaacaccg gcatttatgc ctgttggcac ttatggcacg gtaaaaggta
    12241 tgactcctga agaagtgaaa gcgacagggg cacaaatttt attaggtaac accttccatt
    12301 tatggttaag accggggcaa gaagtgatga aaatgcacgg tgatttgcac gattttatgc
    12361 aatggcacgg tccgatttta accgattccg gcggtttcca ggtttttagc ttaggcaaac
    12421 tgcgtaaaat taaagaagaa ggtgtaactt tccaaaaccc gattagcggt gagaaaattt
    12481 tcctttcgcc ggaaaaatca atggagatcc aatacgatct tggttcagac attgtcatga
    12541 tttttgatga atgtacccct tatccggcaa cctttgatta tgcaaaaaaa tcaatggaaa
    12601 tgtcgcttcg ttgggcgaaa cgttctcgtg atcgctttga tgagcttgaa aacccgaatg
    12661 cgttatttgg cattattcaa ggtggtattt atgaagaact tcgcaaagtc tcggtggaaa
    12721 aattagttga aatcggcttt gacggctatg cagtcggtgg tttggcggta ggtgagccga
    12781 aagaagatat gcaccgcatt ttagaatatg tttgcccgca aattccggca gataaaccac
    12841 gctatttaat gggagtgggc aagccggaag atttagtcga aggggttcgc cgtggtattg
    12901 atatgtttga ctgcgtaatg ccaacccgca atgcccgtaa cggacattta tttgtgacca
    12961 acggcattgt gaaaattcgt aatgcgaaat accgtaacga tattacacct ttagatcctg
    13021 aatgtgattg ctacacttgc aaaaactata ctaaggccta tttatatcac ttagacaaat
    13081 gtggcgaaat tctaggggca agactaaaca ccattcataa tttacgttac tatcaacgct
    13141 taatggcaga aattcgtcaa gccatcgaag aagatcgttt tgatgatttt gtcgtggaat
    13201 tttatgctcg aattggcaaa gaagtgccac ctttacaatc tgaagtaaac ggtcagaaat
    13261 aacaaggagt atatatgcaa caacttgaac cacaaacctt tgcaagctgg gcagagccga
    13321 tcgatatgct ttatgcttgc cacagcaaag taaaaagttt ctgcaaacag ctacaaattt
    13381 tgccggatta ccttgagaaa aacggggtaa accaagcggt caagaatgat gtgcagcaga
    13441 ttttgaatta cttcaacatt tctgcccctt tacatcacga agatgaagaa gatgatttct
    13501 tccctgagct tattaaggtg caaccacaag ctcaagcaga ggtagacgaa ctggaacgcc
    13561 agcacgttca tttacataaa aattgggacg atttgtcggc acagcttgaa gatctgctcg
    13621 caggtaaacg tgaaaatgtg gatagagact taattgagcg ttttgtggca ggttatgatg
    13681 tgcaattgcc attgaagaac cgctttttga gctaggacga gagcatttag cagaagaaaa
    13741 gctctcagca atgggtaaaa ttatggcgga tcgccgtaaa ttataattgc gatgaataag
    13801 acgaaccttg agttcgcctt ttgtgtattg aagatgaatt attctttaga tttaaccgac
    13861 tatgcctgcc cgttaccttt gcttatggca aaaaaagcga tgaacgatct taaaaagggc
    13921 gatagcttgg aaattttact caatcagcag agtagcctta ccgattttga actgcttgcc
    13981 aaagagcaag gttggctttg tgagctacaa gcggttgaaa atcgggtgat ttttaccatt
    14041 gcttaatttg aggtgcattt attgctaccc agtaatgttt ttaacccttc actaataagc
    14101 ccttgccaag ctaaataacc gtgagaggtt gattgttcga cataagtaac cggataacct
    14161 ttagcgagta aaacatcttt taaatggcga gaagtttcta aaatagccga tttcccgctt
    14221 tcataaaaac ctgcactgat gaaaaaacag agtggtaatt tttctttttg gataaattgt
    14281 tgagcaatat agtggctttg ttcactaggt gtgcctttgg gttgccgcca aaatgaaccg
    14341 gatagaatca atgctttact aaatacatta ggatagttga gtacggtgta agcagaggat
    14401 agcccgccat agctagagcc gccaataatt cgctcctttg tttttatatt gagctgtttt
    14461 tcaacaaaag gtacaagttc ttttgccaat acttgcgtaa atatgggatt ggcaggtagc
    14521 tcgacacgtc ttgattcact cgatggattt tcgataaaca ccgctacagt aggcagaatc
    14581 actttctgag cgatcatatt atctaaaatg gttttagttg gcactcgttc ggtatattca
    14641 acaccgtcaa ataaatagag aaccgcatag tttttttctg cactaaaacc gtcaggcaga
    14701 tagacgaata atctgcgttt attgccgagt gttgcactct caaaaatcaa ctgctgcagt
    14761 ttaccttttg caatatttgg agcttcagtc aaaaaagatt gagtaggtgc gtttggcaat
    14821 ttaagtagcg aagcgtagtt gaatttatcg gtattatgaa tattttgtcc gattttctcg
    14881 aagtatgctg ttttattgag tggatcggct tgagccgtag ccagtaacgc tctgcgttgg
    14941 gtattctcat caacgggcaa tgtcggcaca tctggagcta atttgtattc cactaaagtg
    15001 tcattgggca ctatataact tttataccat atatccgttt caccaaagcg ttgcataaaa
    15061 tcgtgttcag tactcacacc gccccaaata cggacatttt tcgttgctcc acgccataaa
    15121 aaagttacac ggctttgctg ttcgtccaat ggttcaatca atggtgtgcc ggcttgtttt
    15181 gctttatccc aaaaatcgtt aattaaagcg gtctgttttt ctggattttt tgcaattttt
    15241 tggctgagtt cttcaagctg tgtattttca attactttag gtggagcaat ttgtgccgag
    15301 attggaatat gccgctcaat cgttaaggta aaatcttggt ctgaaacggt tgcaccattc
    15361 gctagaatag aaaaagtagc tgtgccgctt agttctgatt taaacataaa atcttgttca
    15421 ccaaagacat tttgagccag caggcgagag ctttggcagg aaaaataatc gacaaggaga
    15481 gtggctgttg agccttctga atcaatttgc tttgcacaaa atcattctgt ttaagctcaa
    15541 tggttatatt gttagcataa agcacagatg aaaccaaaaa agtaagaata caagcggtcg
    15601 tttttttcat attttttgca aaagtttaag ccctcatttg agggctgttg agattaaaaa
    15661 tgatagttta acgttgcact taaattgcga tcttttccat agaaacagga aaaattattg
    15721 gcacagcttg cgacatattt tttattcgtc aggttttgtg cgttgagctg taagtttaag
    15781 ttaggcataa aatcataagc tagcatcata tcgtaaagtg tgtatgccgg tacggtaaaa
    15841 gtattttggt tatcgccaaa agtcgttccc atatagcgta cacctacacc ggcggtcagc
    15901 ttatcaaaga atttatattt tgcccaaaga gatgcttgat ggcgaggcac tccccactgc
    15961 gtttttccaa tcgtcgtgcc atctttatct gcagtaatct ctttttttaa gtaagcataa
    16021 gaaggcgcta ttgaaaattt atcggataat tgctgattta aactcacttc aattcctttg
    16081 gtttgcactt caccaatttg ctcataagag cgggtttgcc attgataatt ggctaaatct
    16141 ttttgtgtaa ttttaaatcc tgctaaggtc agtaaggtgt cttgggtagg ttggtatttc
    16201 attcccactt cccattgccc agcagttgtt ggttttaaga aatttccttg agcatctttt
    16261 tcagccgatg gcacaaaaga agtgctgtag ctgatatacg gagcgatacc gttttcaaag
    16321 ttgtaaacta cgcctgtacg ccaagtaaat tttccttctt ggttattctc tgttttactg
    16381 ttcaaacgat cttggttgct gttttttgct cgatcgtaac gaccgcctaa caggaagtca
    16441 aaattaccga tttctatttg atcttgtaga taaaccccaa gttgttttaa atgcttcact
    16501 tcacttgaaa ataagctagg ctcagtaacg gtcacgccat aaacagggtt ctgccaatca
    16561 aaatcagggg caatccccat atagttatca agatcttcac gcactcgttt ataatcaata
    16621 ccggctaaca aggtatgatt tatatttgct gtgctaaata cccattctaa ttggttatct
    16681 aagttgattt cttgggtttt gacatcccaa cggcgagctc tacgttctaa aaccgaaccg
    16741 acatcgcccc aataaactag atttttgaaa ttctcttcgc cattatggta acttgcattt
    16801 tggcggaacg ttaggtttgg cgtaaattta tgtgagaaat tgtaacctac ttgcatttgt
    16861 tcggctttta aaccgtgata gttcagatcg ctgaggaaca tatcataggg tagtttcttc
    16921 ccgtttacgc tattaatcgt tccttctttc ggtaaaaagt tacgatcacc ggctttcggt
    16981 ttttttgcat agtaagcgta aacattaagt tctgtgtttg tagtcggttt ccataaaatg
    17041 cttggagcaa aagcaccgcc tttttctttt acaaattggg tattccagtt tgttcgttct
    17101 aattgaccga ttaaacgagc ggataaggta ttggagagat tttcttcaat atccagcccg
    17161 atttggtatc gttgatggtt tcccaccaca aattgcaaat ggcgatgtgg gttactgccg
    17221 gcttttttgg tgataatatc cattacgcca ccgggattag cttgcccgta taaaatggag
    17281 tttggacctt taatcacatt gatactttct aagaagaacg gatcgatttc aaatgcagtt
    17341 tggtagcgag aaccgtttaa ataggtcgga attgtactgc catcactttt attgccaatg
    17401 cctcgaatgg agatttcgat aagcgaattg ttaccacgat agccacccat cataccggag
    17461 gtataagcca ctgaatcggt aacagaacga acagcttggt catgcattaa atcatcagag
    17521 acaacattga tactaaaagg tgtacgagcc aaagaggtta atgttttcgt tcctacagtc
    17581 gtataaacag gaatatagcc ggtggtagct gccaatttct ctttgacctc aatagcattt
    17641 agttctgctt gagaggttgt ttctgctaat acttgaggag ctacaatcgc taaagtgatt
    17701 agagaaagtg aatatttttt tattttcatt agtttatcct taaataagaa tgattattga
    17761 atataataat atcttgtttt tatgcaaaaa acctttgtaa aataaaattt ttactatgcg
    17821 aaaaaaagtt tttaatgatg aaaacaatct tgattgatga agctaaccaa atgaaaggt
//
LOCUS       DANKBV010000040        17862 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.040, whole genome shotgun sequence.
ACCESSION   DANKBV010000040 DANKBV010000000
VERSION     DANKBV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17862
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.040"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..657)
                     /gene="trkA"
                     /locus_tag="PWY43_001094"
     CDS             complement(<1..657)
                     /gene="trkA"
                     /locus_tag="PWY43_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDL2135983.1"
                     /translation="MKVIILGAGQVGSTLAENLVSEDNDIVLVDNDPTRLDKLKDKHD
                     LQVIRGEYASPQTLRDAGAGDADLLVAVTNSDEVNMIACQIAYTLFNVPTKIARICSA
                     DYVRERERLFNDDVLPIDHIIAPEILVKEDILRLIHYPGALQITHFADELVSIVNVKA
                     YYGGPLVGYPISALRDHLPHIDARVVAIFRQDKAIVPQGSTIIEAGDEVFFICATQHI
                     R"
     gene            complement(657..1988)
                     /gene="rsmB"
                     /locus_tag="PWY43_001095"
     CDS             complement(657..1988)
                     /gene="rsmB"
                     /locus_tag="PWY43_001095"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL2135984.1"
                     /translation="MKKQSFKALSSRAISATIILQVLDQGKSLSTLIPDAQKQLEPKD
                     LPLVQEITFGVCRVLPRLESIIKLLVEKPLKGKTRLVHCLLLVGLYQLLYMRVPAHAA
                     VDEVVNATKTLKLDSFRALTNGVLRRFLREQEEILAKVDKHWQTLHPEWLVNKLKKAY
                     PNWREIVEANNQRPPMWIRVNQQHIKTNDYAALISELVEPEFAKNSENPTACVPKCAL
                     LLERAVNVNQLPHFEQGWATVQDAHAQWSAELLGAENGETVLDACAAPGGKTTHILEK
                     APKAKVMALDIEESRLSRVRENLVRLGQIAQVICGDASKPEEWLEEGVLFDRILLDAP
                     CSATGVIRRHPDIKWLRKEGDIAELATLQGEILKALWQRLKPNGILLYATCSVLPQEN
                     SEQIQRFLQDTPNAKQLEIDFNGEKVLEKQFFPQPNGGDGFFYAKLQKVAE"
     gene            complement(2040..2465)
                     /locus_tag="PWY43_001096"
     CDS             complement(2040..2465)
                     /locus_tag="PWY43_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2135985.1"
                     /translation="MYNKLLIAIDLADLKSAKYVVDTALQMTAKNPNAVYRVVSIIEP
                     VDNSLISAFLPKNFDKEVVAEANQKLHEFTKNHFPEGSKVQHIVAYGTIYEEICRIAD
                     EKSVDLIMMLASSKPNAKGLSSNTVKVARNTTKPILVLR"
     gene            complement(2558..5206)
                     /locus_tag="PWY43_001097"
     CDS             complement(2558..5206)
                     /locus_tag="PWY43_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HDL2135986.1"
                     /translation="MSVESKVDYDDLQDMVASNDSGGRTPSGLAKKAIVYVAILWSVF
                     QIYYASPLPFVFQEWLTHAGINLNVVVDDTKARSIHLAFAMFLAFLSYPAFASSPKHH
                     IPKIDWVFAIVGAGLALYYIFFYEGLVKRFGAPNTQDIIAGCLGILLVLEATRRSLGL
                     PLVIIAIVFLIYNFFGQYFPADWIVSHRSGSLSQIINQQWITTEGVFGVALGVSTKYV
                     FLFVLFGALLDKAGAGNYFIKTAFAYLGHLRGGPAKAAVVSSALTGLISGSSIANVVT
                     TGTFTIPMMKRVGFTKEKAGAVEVASSVNGQIMPPVMGAAAFLMIEYVNMPYSQLITH
                     AFLPALISYIALVYIVHLEACKMNLQGLPRAEEPSPILVFLLRTVASILVIVGLAIGV
                     YYGLGWIRDVAEDYAFMIVCAFLGLWYLLAIRRVASYPDLEADDPNSEIVSLPKRKPT
                     VNAGLHYLIPVVVLLWCLMVEMLSPGLSAFWGTITLIFIQLTQRPLLNFFRKESVTKE
                     IIKQGVRDVVDSLEAGGRNMTGIALATATAGIIVGVVALTGFGVQLSSVIELLSMGNI
                     VLMLILVAVFSLILGMGLPTTANYIVVSSLMATVIMEVGRQNGLIVPLIAVHLFVFYF
                     GIMADVTPPVGLASFAAAAISGGSPINTGMVAFKYSLRTVILPFLFIFNTDLLLIDLR
                     YPGHGWVVFITATVGVLAFTSATMQYIVTKNKWWETALLVVAAFALFRPGFFMDRIYP
                     SERHIEPVQFEEQLLASKVGQNVTLKVEGLNPYGKEIEFYAQLPVPEGATGEERLKNL
                     GLTLIQTDEKIKVDGVETPKVVIDMVEMDSPAAKAGLNWDQTIHYIAVPQDAPSKDWI
                     FIPALLLVFGIVANQRRRVKKQCVIA"
     gene            complement(5408..6370)
                     /locus_tag="PWY43_001098"
     CDS             complement(5408..6370)
                     /locus_tag="PWY43_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HDL2135987.1"
                     /translation="MKKLVKLSFLASLVMGTASVQAAETFVTIGTGGQTGVYYVVGQS
                     ICQLVNRDSAKTGLKCNAPSTGASVANLNAIAAKEMDMGIAQSDWQYHAYNGSSSFEG
                     KKNDKIRAIFSLHPEPFTVMARTDSGIKSFDDLKAKRVNVGDPGSGTRATMNVILAAK
                     GWTDKDFKVASELKPAEMASVMCDNNLDAITYNVGHPNGALKEAAASCDAKLVPVTGP
                     EIDKLVADHSYYAKATIPGGLYKGSDEPTETFGVYATLVTSADVDADKVYNVTKAVFD
                     NFDRFKRLHPAFEHLKQEEMIKNALSAPLHEGAIRYYKEKGWIK"
     gene            complement(6554..8401)
                     /gene="selB"
                     /locus_tag="PWY43_001099"
     CDS             complement(6554..8401)
                     /gene="selB"
                     /locus_tag="PWY43_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDL2135988.1"
                     /translation="MIICTAGHVDHGKTSLLQALTGTNTAHLPEEQKRGLTIDLGYAY
                     LPIENDILGFIDVPGHQRFLSNMLAGLGGIQHALLVISAEEGIKPQTEEHLDILHLLN
                     FQHIMIVITKADRAEETQISHLIEQVKTSYPFLAEAKTFVTSAKTQQGIEELKAHLIQ
                     LNQQHTQTHKPFRYAIDRVFNVKGAGLVVTGTTVAGKVSVGSELFLSNGKKVRVKAIH
                     AQNTPSETGVAGQRLALNIANVEKEEIKRGDWITELEPKFATDRITVQLTANQAFKEN
                     SIVHLYHFASHITGKLNLLEGQQAVKNQQFFAEVILDEPLHIAVGDKLIIRSGDDSLT
                     LAGAEVLEIHSPKRHKRTEARLALVKNLAKTTACNDFATRLDLYLQNRAMDLSLLLWA
                     EQCFAEDIAKLGFDVSSKWLFNSDFKAQAQQRVLEKMAEYHLQHQDQIGVTKARLYRI
                     ALLDLPENLANQFIEDLVEQKALVNSRGWLHLPEHRIELNADELQIWQQIRPLFEATN
                     QALWVRDIASQLSIDETQMRNLLYKVGKLGYLIPIVKDRFLLSEQIGQFATLIKDFIA
                     QNGSISVNQLRDELQYGRKLTVQLIEYFDRSGFLRRKGDVHLLRDSETF"
     gene            complement(8411..8707)
                     /locus_tag="PWY43_001100"
     CDS             complement(8411..8707)
                     /locus_tag="PWY43_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2135989.1"
                     /translation="MMKFDYNIVWLQKAEIKLFYQADYILQQSQNPIIAEQFYDAIKS
                     EVDKLSFTADVYRLRKKKEIPILNGKYQVKFLIGQENVYIVDFKSARQNSYSFL"
     gene            complement(8704..8880)
                     /locus_tag="PWY43_001101"
     CDS             complement(8704..8880)
                     /locus_tag="PWY43_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2135990.1"
                     /translation="MELILAQDLAKLRTDKPQSFYTNDELDLLHQLENGLIETKSHAQ
                     VMEDLRKKLGITEK"
     gene            complement(8949..9581)
                     /gene="pdxH"
                     /locus_tag="PWY43_001102"
     CDS             complement(8949..9581)
                     /gene="pdxH"
                     /locus_tag="PWY43_001102"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDL2135991.1"
                     /translation="MDLHHIREDYTKKVLSIRDCHSNPITQFEQWLSESINAKVNEPT
                     AMNIATVAANGRPSSRMVLLKEVNSQGFVFFTNYQSRKGREIADNPFGCLTFFWAELQ
                     RQVRIEGKIAKIPEAESDAYFDSRPYTSRLGAWASEQSQPIENYKALLLKSAQFALKY
                     PLSVPRPPHWGGYILIPDSIELWQGRSSRLHDRIHYWLENNEWKKERLSP"
     gene            complement(9639..11021)
                     /locus_tag="PWY43_001103"
     CDS             complement(9639..11021)
                     /locus_tag="PWY43_001103"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDL2135992.1"
                     /translation="MQQLFRQIPSLDLLLKKPQAVELCQKFAYSSVAEQARSLLTQAR
                     QFISQQQALPDFIADENLFFIRLEQKVQALSEVKIRSVFNLTGTVLHTNLGRGLWSEK
                     AIQAATNAMRNNVALEFDIEAGKRSHRDNYISELLQILTGAEAACIVNNNAAAVLLML
                     ATFAQGKEVIVSRGELVEIGGAFRIPDIMAQAGCKLVEVGTTNRTHLKDYRNAITENT
                     AFLMKVHTSNYHIEGFTSSVSEEELVALGKEFNLPVITDLGSGSLTDMQALNLPAEPK
                     VQQKVAAGVDLVSFSGDKLLGGPQAGIIVGSQAMISQLQQHPLKRVLRCDKVILSALE
                     ATLRHYLFPEQLTDNLPTLHLLTQPLETLQHKAERLKQALHKRLNSAYILQIEPSLAQ
                     IGSGALPTETLPSVAVTISAEKQSDLIALEQQFKTYPSPIIGRFAQQKFWLDLRSVVE
                     FDKLIEMLEY"
     gene            11089..11183
                     /locus_tag="PWY43_001104"
     tRNA            11089..11183
                     /locus_tag="PWY43_001104"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11123..11125,aa:Sec,seq:tca)
     gene            11390..12079
                     /locus_tag="PWY43_001105"
     CDS             11390..12079
                     /locus_tag="PWY43_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2135993.1"
                     /translation="MSTLSRDSILSQIQNGLIASCQPVDDGPMDKPEIVAAMAAASVI
                     GGAKGIRIEGVDNLKAARAAVNVPIIGIVKRDLPDSPVRITPFLEDIDALAEAGADII
                     AVDGTFRTRPVSIEEAVKRIHEKGCLAMADCSTLEEGLHCQQLGFDIVGSTMSGYTGG
                     EVPDEPDYQLVKDLKAAGCNVMAEGRYNTPELAKTAIEIGADYVTVGSALTRLEHIVS
                     WFADSVKSAQK"
     gene            12089..12991
                     /locus_tag="PWY43_001106"
     CDS             12089..12991
                     /locus_tag="PWY43_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDL2135994.1"
                     /translation="MRCLAIDIGGTKIATAIVQHNQVSQRQQIQTPTDKPQADQAGSL
                     HQAIGEIMQHYQGQFDFVAVASTGIINKGVLTALNPKNLGGLAEFPLKQSIEKHTDKP
                     IALLNDVQAAVCAEYLNEDQQAVKNFVFITVSTGVGGGIILNGELQIGSNGIAGHIGH
                     TLADPNGPLCGCGRVGCVEAVAAGRAIEAVSSQWEKPCSPKEVFERFRQGNPQAVELV
                     QKSAKAIANLIADLKISLDIQKVVLGGSVGLAEGYLPQVSNYLAEMPSVYHCILENAL
                     SGQDAGLIGAAWWAENQLNQGFTL"
     gene            13016..13894
                     /gene="nanA"
                     /locus_tag="PWY43_001107"
     CDS             13016..13894
                     /gene="nanA"
                     /locus_tag="PWY43_001107"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDL2135995.1"
                     /translation="MKNLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYV
                     GGSTGENFMLSTEEKKEIFRIAKDEAKDEIALIAQVGSVNLKEAVELGKYATELGYDS
                     LSAVTPFYYKFSFPEIKNFYETIIRETGNKMIVYSIPFLTGVNIGVSQFAELFENEKI
                     IGVKFTAGDFYLLERLRKAFPNHLIYAGFDEMMLPATVLGVDGVIGSTFNVNGIRARQ
                     IFELAQQGKIKEAFEIQNVTNDLIEGILGNGLYQTIKGLLELEGVQAGYCREPMTKEQ
                     TPAQKEVTKQLKAKFL"
     gene            complement(13955..14160)
                     /locus_tag="PWY43_001108"
                     /pseudo
     CDS             complement(13955..14160)
                     /locus_tag="PWY43_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14118..14763)
                     /locus_tag="PWY43_001109"
                     /pseudo
     CDS             complement(14118..14763)
                     /locus_tag="PWY43_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14868..16463)
                     /locus_tag="PWY43_001110"
     CDS             complement(14868..16463)
                     /locus_tag="PWY43_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2135996.1"
                     /translation="MRLSQLSLSVLLLSASSLVAAAPVNTLINCVATPPMKLSPAITN
                     DANDFNASSQQIYNRLLAFKAGELTVEPSLAERWEISDDGLSYTFHLRKDVKFHSNKQ
                     FTPTRTLNADDVVFSFQRQMDLNHPYHKVSDGTYFYYHWMNLPKILKTVEKVDDYTVK
                     ITLQQPNAPFLTTLAMDFLSIYSKEYADKLLAEGKPELLDQAPIGTGPFVFQAYQTDQ
                     AVRYVANPDYFQGKAKFEKLIFSITPDAGTRYAKLKAGECDVIDFPNISDIAQMKQDP
                     KVNLLQRDGLNLAYIGLNSTKPALNNAKVRQALHYATDKKAIVEAVYQGGGTVASNPF
                     PDSVLGYNPNLPQYEFNLEKAKALLTEAGFADGFETEIWVQPVVRPSNPNPRRTAEII
                     QADWAKIGVKAKLVTHEWADFNKRTREGEFSTGTYGWTSRNGDPDNFLFPLLSKSNIP
                     GTNYGRWTDEKFEQLLAQAVQTQNRDERAKLYQQAVEIANEQTPFIPLAHAINYVPLS
                     KRVQGFVQNPFGYTPFYGVSLSE"
     gene            16612..17052
                     /gene="mioC"
                     /locus_tag="PWY43_001111"
     CDS             16612..17052
                     /gene="mioC"
                     /locus_tag="PWY43_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDL2135997.1"
                     /translation="MTSICIITGSTLGGAEYVADHINEVLSEQGFEVELFNQATLADI
                     EGKANLIVVTSTHGAGELPENIQPLFDDLANSTADFSSMKFGVIGLGSSDYDTFCNAV
                     NIVEEALKAKGASQVCESLRIDVMNNFDHDGSAEEWLPSFIEQL"
     gene            17064..17231
                     /locus_tag="PWY43_001112"
     CDS             17064..17231
                     /locus_tag="PWY43_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDL2135998.1"
                     /translation="MKKISANKAKWIKIAIILIYMFSPVDILPEAILGPLGLVDDAAA
                     LMLLIKTILEK"
     gene            complement(17320..17793)
                     /gene="asnC"
                     /locus_tag="PWY43_001113"
     CDS             complement(17320..17793)
                     /gene="asnC"
                     /locus_tag="PWY43_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDL2135999.1"
                     /translation="MYSKNTTKSEIDKLDQKILRWLIKDARTPYAEMAKVFGVSAATI
                     HVRVEKMRQAGIIESTKIVVDERKVGYDVCCFIGIILKSAKDYDKVIAKLSEFNEVVE
                     AYYTTGNYSIFIKVMTHTIEELHSVLAGKIQSIDDIQSTETLISLQNPISRDILP"
BASE COUNT         5166 a         3950 c         3586 g         5160 t
ORIGIN      
        1 tcgaatatgt tgggttgcac agatgaaaaa gacttcatca cctgcctcaa taatagttga
       61 accttgtggc acaatcgctt tgtcttggcg gaaaattgcc acgacccgag catcaatatg
      121 cggtaaatga tcacgcaggg ctgaaatcgg gtaaccgacc aaaggcccac cgtaataggc
      181 ttttacattc acgatactga ctaactcatc agcaaaatga gtaatttgca acgcccccgg
      241 atagtgaata aggcgtaaaa tatcttcttt gactaaaatt tcaggggcaa taatatggtc
      301 aataggcaat acatcatcat taaacaaccg ctctcgttct ctcacataat cagcactgca
      361 aatacgggca attttggtcg gcacattaaa taaagtgtaa gcaatttggc aagcgatcat
      421 attcacttca tcactattag ttacagcaac taatagatca gcatcacctg caccggcatc
      481 acgcaaggtt tgaggtgaag catattcgcc ccgaatcacc tgtaaatcgt gtttatcttt
      541 gagcttgtct aaacgagttg ggtcattatc caccaagaca atatcattgt cttcactcac
      601 caaattttct gccagcgtag aaccaacttg tcctgcccct aaaataatta ctttcattac
      661 tctgccactt tttgtagttt tgcgtagaag aaaccatcac caccgtttgg ttgtgggaag
      721 aactgttttt ctaacacttt ttcgccgtta aaatcgattt caagttgttt tgcattcggt
      781 gtatcttgta aaaaccgttg aatttgctcg ctattttctt gcggtaacac cgagcaagtg
      841 gcatataaca aaatgccgtt tggctttaac ctttgccata acgccttgag aatctcaccc
      901 tgtaatgtgg ctaattctgc aatatcaccc tctttacgca accatttaat atcagggtgg
      961 cggcgaatga ctccggttgc ggaacaaggg gcatcaagta aaatgcggtc aaacagcaca
     1021 ccctcttcaa gccattcctc aggtttggaa gcatcgccac aaattacctg tgcaatctgc
     1081 cccagtcgga ctaagttttc acgcactcgg cttaaacggc tctcttcaat atccaacgcc
     1141 ataacctttg ctttcggtgc tttttctaaa atatgggtag ttttgccacc cggtgcggca
     1201 caggcatcta aaacggtttc accattttct gccccgagca actccgctga ccattgtgcg
     1261 tgagcatctt gcacggttgc ccaaccttgc tcgaaatgtg gcagttggtt cacatttacc
     1321 gctctctcca gcaataaagc acactttggc acgcaagcgg tcggattttc tgaatttttt
     1381 gcaaattcag gctcaaccag ctcgctaatc aacgccgcat aatcattggt cttgatatgt
     1441 tgttgattga ctcgaatcca cattggtggg cgttggttgt ttgcttcaac aatttctcgc
     1501 cagttcggat aggctttttt aagtttattg accaaccatt cagggtggag cgtttgccaa
     1561 tgtttatcta ccttcgctaa gatttcttct tgctctcgca gaaaacggcg tagcacaccg
     1621 ttagtcaagg ctcggaagct atccagtttt agcgttttag ttgcattcac tacctcatca
     1681 accgcagcgt gtgccggcac tcgcatataa agcagttggt ataaacccac taacagtaag
     1741 caatgcacca aacgggtttt gcctttcagc ggtttttcaa ctaataattt gatgatcgat
     1801 tccaagcgag gcagcacacg gcacactccg aaggtgattt cttgcactaa gggtaaatct
     1861 tttggttcaa gctgtttttg agcgtcagga atcaaggtgg aaagtgattt accttgatct
     1921 aacacctgta aaataatggt tgcacttatt gctctagaag aaagtgcttt gaacgattgt
     1981 tttttcataa ataaaaatgc cattccgaca gaatggcact attgtaactg aatgcgggat
     2041 tatcttaaaa ctaaaattgg cttggtggtg ttgcgagcca ctttaaccgt attagagctt
     2101 aagcccttcg catttggttt gctgcttgcc agcatcataa ttaaatccac cgacttttca
     2161 tcggcaatac ggcaaatttc ttcataaatc gtaccatacg ccacaatgtg ctgcactttt
     2221 gaaccttcag gaaaatggtt tttggtaaat tcgtgcaatt tttggtttgc ttcagccacc
     2281 acttctttat cgaaattttt cggcaagaat gcagaaatta agctgttatc caccggctca
     2341 ataatagaca caacccgata aaccgcattc ggattttttg ccgtcatttg taatgccgta
     2401 tctaccacat atttagcact ttttaggtcg gctaaatcaa tcgcaataag taatttgtta
     2461 tacatattta ttccgtaggg gcaggtcctg tgcctgcccg aatattaact aaaatagcgg
     2521 acgggcacag gacccgtccc tacatagcat ataaatacta tgcaataaca cactgttttt
     2581 tcactctacg gcgttggttt gccacaatac caaataccaa caatagagca ggaatgaaga
     2641 tccaatcttt agaaggagca tcttgcggta cggcaatgta gtgaatggtt tgatcccagt
     2701 ttaagcctgc tttagctgcc ggagaatcca tttctaccat atcaatcacc acttttggtg
     2761 tttctacgcc atcaactttg attttttcat cggtttgaat tagcgttaag cctaagtttt
     2821 tcaaacgctc ttcgccggtt gctccttccg gtacaggtaa ttgagcgtag aactcaattt
     2881 ctttaccgta agggtttaat ccctctactt ttaaagtaac attttgacct actttgcttg
     2941 caagcaactg ttcttcaaac tgcaccggct cgatatgacg ttcagacgga taaattctgt
     3001 ccataaagaa gcccggacgg aacaaggcga aagcggctac aaccagcaac gccgtttccc
     3061 accatttgtt tttagtcaca atatattgca ttgtagcaga ggtaaaggcg agaaccccaa
     3121 cagttgcggt aataaacaca acccagccgt gccccggata acgcagatca attaataata
     3181 aatcggtatt aaaaataaat aagaacggta aaatgacggt tcttaaacta tatttaaatg
     3241 ccaccatacc ggtattaatc gggctaccgc ctgaaattgc tgccgcagcg aacgaggcta
     3301 aaccaacagg tggagtcaca tctgccataa tcccaaaata gaatacgaat aaatgcaccg
     3361 caatcaacgg cacgattaac ccgttttgac gaccgacttc catgataacc gttgccatta
     3421 atgatgacac cacaatgtag tttgcagttg tcggtaagcc cattcccaaa attaagctga
     3481 atacggctac caagatcaac attagcacaa tgttgcccat tgataggagt tcaatcacac
     3541 ttgaaagctg aaccccaaag cccgttagtg ccacaacgcc gacaataatc cctgctgttg
     3601 cggtagcgag ggcgataccg gtcatattac gaccgccagc ctcaaggcta tctaccacat
     3661 ctcgaacacc ttgtttgata atctctttgg ttacgctctc tttacggaag aaattcagta
     3721 acgggcgttg ggttaattga ataaaaatta aggtaatggt tccccagaat gcggataagc
     3781 ccggcgaaag catttccacc attaagcacc aaagtagcac aaccacagga ataaggtaat
     3841 gtaaacctgc atttacggtt ggttttcttt ttggtagaga aacaatttct gagtttggat
     3901 cgtccgcttc taaatcaggg tagcttgcca cacggcgaat agccagtaga taccataagc
     3961 ctaagaaagc acagacaatc ataaacgcat aatcttctgc cacatcacga atccaaccta
     4021 agccgtagta aacgccaatc gctaatccta cgatcactaa aattgaagct acggttctta
     4081 gcaagaagac taaaatcggg cttggctctt cggctcttgg taagccttgt aaattcatct
     4141 tacaagcttc taaatgcacg atatacacca acgcaatata cgaaattaac gcaggtaaga
     4201 aggcgtgagt aatcagctgg ctatacggca tattcacata ttcgatcatc aagaacgctg
     4261 cagcccccat taccggtggc ataatttgac cgtttactga tgaggcaact tccaccgcac
     4321 cggctttttc tttggtaaag ccgacccgtt tcatcattgg aatagtgaat gtacctgtcg
     4381 tcacgacgtt tgcaatcgaa gagcctgaaa tcaagccggt taaagcggaa gaaaccaccg
     4441 ccgcttttgc cggaccacca cgcaaatgcc ctaaataggc aaaggcggtt ttaataaagt
     4501 agttacctgc tcctgcttta tcgagcaacg ccccaaataa tacgaacagg aacacatatt
     4561 tggtcgaaac cccaagggct acaccaaaca cgccttcagt ggtaatccat tgttggttga
     4621 taatttgaga aagcgaacct gagcggtggc ttacaatcca atctgccggg aaatattgcc
     4681 cgaagaagtt atagatcagg aatacaatcg caataatcac aagcggtaag ccaagactac
     4741 ggcgggtcgc ttccagcacc aataaaatcc ctaaacagcc ggcgataata tcttgcgtat
     4801 ttggcgctcc aaaacgtttc accaaacctt catagaagaa aatataatac aacgctaacc
     4861 cagctcctac gatggcaaac acccaatcta ttttaggaat atgatgttta ggcgagctgg
     4921 caaaagcagg gtaagatagg aacgccaaga acattgcaaa ggctaagtgg atagaacggg
     4981 ctttggtgtc gtccaccacc acattcaaat taatgcctgc gtgagtaagc cattcttgga
     5041 atacaaacgg caatggagaa gcataataaa tttggaacac agaccaaaga atggcgacat
     5101 acacaatggc ttttttcgca agaccggacg gggttcgccc cccactgtca ttggaggcaa
     5161 ccatatcttg taggtcatcg taatcaacct tagattcaac tgacataaag actcctcaaa
     5221 acgcaagcgg tcattttctt attaaatttt acaaatttcc tcaaaaaaca accgcttgct
     5281 cgtgaaaaca ctgttgataa gttatcaatt aaaggtgaaa atcgataact tatcaacagt
     5341 ttcaaaaaag caaaaaacca gcacgctaaa aaacgtgctg attgaatcaa attacagaaa
     5401 ggaaatatta cttaatccaa cctttctctt tatagtaacg aattgcacct tcgtgtaacg
     5461 gagcagaaag ggcgtttttg atcatctctt cttgttttaa gtgttcaaac gccggatgta
     5521 aacgtttgaa acggtcgaag ttatcaaata ccgctttggt tacattgtac actttatctg
     5581 catccacatc ggcagaagtc actaacgttg catatacacc gaaggtttct gtcggctcat
     5641 ccgaaccttt atataaaccg cccggaatcg tagctttggc ataatatgag tgatctgcca
     5701 ctaatttatc aatttcaggg ccggttaccg gcactaattt cgcatcgcaa gaagcggctg
     5761 cttcttttaa cgcaccattc gggtgaccta cgttataagt gatggcatct aagttgttat
     5821 cacacattac agatgccatt tctgccggtt tcaattcaga agccactttg aaatctttat
     5881 ccgtccagcc ttttgccgct aaaatcacgt tcattgttgc acgagtaccg gaacccgggt
     5941 cgcccacgtt tacacgtttt gcttttaaat catcgaacga tttaatgcct gaatcggtac
     6001 gagccattac tgtaaacggt tccgggtgaa gcgagaaaat ggcacgaatt ttatcgtttt
     6061 tcttaccttc aaatgagctt gagccgttat aggcgtggta ttgccagtct gattgtgcaa
     6121 tccccatatc catttctttt gctgcaatcg catttaagtt tgcaaccgat gcccctgttg
     6181 aaggagcgtt acattttaaa cctgtttttg ctgaatcacg gtttactaat tggcaaatag
     6241 attgacccac aacataataa acaccggttt gaccgcctgt tccgatagtc acaaatgttt
     6301 cagccgcttg aacagatgct gttcccatca caaggcttgc aaggaaagaa agtttgacta
     6361 attttttcat ttttaactcc taaaattaag gatcagaaaa caaagattta ttgttttgag
     6421 ccactgctca cactacaaga gatacaaaac ttctttgagt tgcatttgcc tcttagagct
     6481 tgtttttagc atagatttta taattagtaa atttattttt tcgcataaaa aaatctatgc
     6541 ctaatgccac aagctaaaat gtttcactat ctcttaacaa atgcacatcg ccttttcgtc
     6601 ttaaaaaacc gctacgatca aaatattcaa ttaattgtac cgtcaatttt cgcccatact
     6661 gcaactcatc tcgcaactgg ttcaccgaaa tgctgccgtt ttgggcgata aaatctttaa
     6721 ttaacgttgc aaattgcccg atttgttcgc ttaataaaaa tctatctttg acgattggga
     6781 ttaaatagcc caatttcccg actttataca gcaaattccg catttgggtt tcatcaatcg
     6841 agagctggct tgcaatatcc ctcacccaaa gagcttgatt ggttgcctca aacagcggtc
     6901 gaatttgttg ccaaatttgc aattcatcgg cgttcaattc gattcgatgt tccggcaaat
     6961 gtaaccaacc acgactattg accaaggctt tttgctccac caaatcctca ataaattgat
     7021 ttgccaagtt ttcaggcaaa tccaataacg ctatgcgata taaacgagct ttggtcacgc
     7081 cgatttgatc ttgatgttgc aggtggtatt ctgccatttt ttccaacacc cgttgctggg
     7141 cttgggcttt aaaatccgaa ttaaataacc atttagacga gacatcaaac cccagttttg
     7201 caatatcctc tgcaaaacat tgctccgccc ataacagcaa ggataaatcc atcgctctgt
     7261 tttgcagata gagatcaagg cgtgtcgcaa agtcattaca agcggtggtt tttgctaaat
     7321 tttttaccaa agctaaacgg gcttcggtac gtttatgccg ttttggtgag tgaatttcca
     7381 gcacctccgc tccggctaac gttaggctgt catcaccgct gcggatgatc aacttatcac
     7441 caacggcaat atgaagtggt tcatctaaaa tcacttctgc aaaaaattgc tgatttttga
     7501 ccgcttgttg cccctcaagc aaattcaatt tgccggtaat gtgcgaagca aaatggtaaa
     7561 gatgcacaat gctgttttct ttgaaggctt gattggcagt gagttgaacc gtaatgcgat
     7621 cggtggcaaa tttcggctca agctcggtaa tccaatcgcc tcgcttgatt tcctcttttt
     7681 ccacgttagc gatatttaac gccaaacgtt gccctgccac tcccgtttcc gaaggcgtat
     7741 tttgggcgtg aatcgctttg acccgcactt ttttaccatt cgacaaaaat aactcagagc
     7801 caacgctcac ttttcccgcc accgttgtgc cggtaacgac caaccctgct cctttcacat
     7861 taaacacacg gtcaatcgca taacggaacg gtttatgggt ttgggtatgt tgttgattta
     7921 attgaattaa atgtgctttt aattcttcaa tcccttgctg cgtttttgcc gaagtaacaa
     7981 aggtttttgc ttccgctaaa aacggatagc ttgttttcac ttgctcaatt aagtggctga
     8041 tttgagtttc ttccgcccga tctgccttag taatcacaat cataatatgc tggaaattca
     8101 gcaggtggag aatatccaaa tgctcttcgg tttgtggctt aatcccttct tcggcagaaa
     8161 tcaccaacag ggcgtgttga atgccgccaa gccctgccag catattagat aaaaaacgct
     8221 gatgacccgg cacatcaata aaccctaaaa tatcgttctc aatcggtaaa taggcatagc
     8281 ccaaatcaat ggttagccca cgtttttgtt cttcgggcaa atgggcggtg ttagtgccgg
     8341 ttaaggcttg taaaagcgat gttttgccgt ggtcaacgtg accggcggtg cagatgatca
     8401 ttgatagtcc ttataaaaat gagtaactat tctggcgagc agatttaaaa tctacaatat
     8461 agacattttc ctgaccaatc aggaacttta cttggtactt accgtttaaa atgggaattt
     8521 cttttttctt gcgaagtcga taaacatcgg cagtaaagga aagtttatcc acttcacttt
     8581 ttattgcatc ataaaattgt tctgcaataa tcgggttttg ggattgttgt aatatataat
     8641 ctgcttgata aaacaatttt atttctgctt tctgtagcca aacaatattg taatcaaact
     8701 tcatcatttt tccgtgattc ccaatttttt gcgtaaatct tccatcacct gagcgtggga
     8761 tttagtttca attaagccat tttctaattg atgtaataaa tcaagttcat cattagtata
     8821 aaaactttgt ggcttatctg tgcgtaattt tgctaaatct tgtgcgagta tgagttccat
     8881 atttccccct tacaaactta gattattcaa tattgtaacc tgataaaact aaagttcaag
     8941 gagtgtattt aaggcgataa ccgctctttt ttccactcat tattttccaa ccagtaatga
     9001 atgcggtcgt gtagccggct acttctgcct tgccagagtt caatactgtc gggaatcaga
     9061 atatagccac cccaatgcgg tgggcgaggc actgaaagtg gatatttcaa tgcaaattga
     9121 gctgatttta gcaacaaagc cttgtagttt tcgataggct gactctgctc gctcgcccaa
     9181 gcccccaaac ggctggtgta aggacgagag tcaaaatagg catcggattc tgcctctgga
     9241 atttttgcta ttttcccttc aatccgcact tgacgttgta attctgccca aaagaaggtt
     9301 aaacatccaa aaggattatc ggcaatttcc cgccctttac ggctttgata attggtaaag
     9361 aacacaaagc cttgtgaatt tacctctttc aacagcacca ttcggctact tggtcgcccg
     9421 tttgctgcaa cggtagcaat attcattgct gtcggctcat taactttggc attgattgac
     9481 tcactcaacc attgttcaaa ttgggtgatt gggtttgagt gacaatctcg gattgaaagc
     9541 acttttttgg tgtaatcttc tcgaatgtgg tgcagatcca taaggattcc tttatcttat
     9601 tctaaataat cggacaggca cagtctgccc taccaaaatt aatattccaa catctcaatc
     9661 aacttatcaa actccacgac cgagcgtaaa tctaaccaaa atttctgctg agcaaaacgc
     9721 ccgataatcg ggcttgggta ggttttaaat tgttgttcca atgcaattaa atcgctctgt
     9781 ttttcagcgg aaatagtcac tgccaccgaa ggtaaggttt ctgtgggtaa cgcaccgctg
     9841 ccgatttgag cgaggcttgg ctcaatttgc aaaatataag cagaatttaa ccgcttgtgt
     9901 agtgcctgtt ttaatcgctc tgctttgtgt tgcaaggttt caagcggttg agtgagtaaa
     9961 tgaagagtcg gcaagttatc ggttaattgc tccggaaata agtagtggcg tagcgttgcc
    10021 tcaagtgccg ataaaatcac tttatcgcaa cgtagcaccc gttttagcgg gtgttgttgc
    10081 agttggctaa tcatcgcttg gctaccgaca ataatgccgg cttgcggccc gcctaataat
    10141 ttatcgcccg agaaggaaac caaatctacc ccagccgcta ctttttgctg aacctttggc
    10201 tcagcaggca aattaagagc ttgcatatca gttagcgaac cactgcctaa gtcggtaatc
    10261 acaggcaggt taaactcttt accgagagcc accaattcct cttccgacac tgaactggtg
    10321 aacccctcaa tgtggtagtt actggtgtga actttcatta aaaaggcggt attttcggtg
    10381 atcgcgttgc gataatcctt taaatgagtg cgattagtcg taccaacttc caccaatttg
    10441 caaccggctt gagccataat atccggaatg cggaatgccc cgccaatttc caccagctcg
    10501 ccacgggaaa cgatgacttc cttgccttgt gcgaaggttg ctaacatcaa caacaccgct
    10561 gccgcattat tattcacaat gcaagcggcc tccgctccgg taagtatttg caacaactcg
    10621 gaaatgtagt tatctcggtg cgagcgcttg cctgcttcaa tatcaaattc caacgccaca
    10681 ttgtttcgca ttgcgttggt tgctgcctgt atcgcctttt ccgaccacaa cccacgccct
    10741 aagttggtgt gtaacaccgt accggttaag ttgaacacgg aacggatttt cacctcactc
    10801 aacgcttgca ctttttgttc cagccttata aaaaacaggt tttcatcggc aataaaatcc
    10861 ggcaaggctt gctgttggga aataaattga cgtgcttgag taagcaaact cctagcttgt
    10921 tcagccacgc tggaatatgc aaatttttgg cataattcga ccgcttgtgg ctttttcaac
    10981 aagaggtcga gcgagggaat ttggcgaaat agttgttgca taatgtgaat gattgagaga
    11041 atattttgga caattctaat aaataaagta caatattgcc aatttttcgg aagtgcgtca
    11101 tctccggtga ggtggcagga cttcaaatcc tgttgaggac gccagcgttc tcgggtgggt
    11161 tcaactccca tgcacttccg ccacatttca aaagccgtta atctttaacg gctttttgca
    11221 tttctgccac attctctctt tccgttttga taaaaaatga gacttagctc acattttttt
    11281 atttttctgt ttgtagtaaa aatttattaa attataatta tttggagtat ttcttcacaa
    11341 acaaaatcta gtccattgac taccttttga acataaaaaa ggaactgtta tgtcaacact
    11401 atctcgtgat tctattttaa gtcaaattca aaacgggctg attgcctcct gccaaccggt
    11461 tgatgacggc cccatggata aacctgaaat tgtcgccgca atggcagcag cctctgtgat
    11521 tggtggtgct aaaggcattc gcattgaagg tgtcgataat ttaaaagccg ctcgtgcggc
    11581 agtgaatgtg ccgattatcg gcattgtaaa acgtgatttg ccagattccc ctgtgcgtat
    11641 tacgccattt ttagaagata ttgatgcctt agccgaagcc ggtgccgata ttattgcggt
    11701 ggacggcaca ttccgcaccc gcccggtcag cattgaagag gcggtaaaac gcattcacga
    11761 aaaaggttgc ttggcaatgg cggattgctc cacccttgaa gaaggtttac attgccaaca
    11821 attaggcttt gatattgtgg gtagtacaat gtctggctac accggtggcg aagtgcctga
    11881 tgagccggac tatcaattag tgaaagattt aaaagctgcc ggctgtaatg ttatggcgga
    11941 aggtcgctac aacacgcctg aactggcaaa aacggcgatt gaaatcgggg cggattacgt
    12001 taccgttgga tcggcattga cccgcttaga gcatattgtg agctggtttg ccgactctgt
    12061 aaaatcggct caaaaatagg agctgcttat gcgttgttta gcaatagaca tcggtggcac
    12121 gaaaattgcc acggcaattg tgcaacacaa tcaagtgtca caacgccaac aaattcagac
    12181 cccgactgat aaaccacaag ctgatcaggc aggcagtttg catcaagcga ttggtgagat
    12241 aatgcaacat tatcaagggc aatttgattt tgtggcagtt gcctcaacag gcattatcaa
    12301 taaaggggtg cttaccgcat taaatcctaa aaatttaggc ggattggcag aatttccgtt
    12361 gaaacaaagc attgaaaaac ataccgataa gccgattgca ttattaaatg acgtgcaagc
    12421 ggcggtgtgt gccgaatatt taaatgaaga ccaacaagcg gtcaaaaatt ttgtatttat
    12481 taccgtttcc accggcgtag gcggcggcat tattttaaac ggcgaactgc aaatcggcag
    12541 taacggcatt gcaggacata tcgggcatac tctagccgat ccgaacgggc ctctctgtgg
    12601 ttgtggacga gtcggctgtg tcgaagcagt cgcagcaggg cgtgcaatag aggcagtttc
    12661 cagccaatgg gaaaagcctt gttcaccgaa agaggtgttc gagcgattcc gccaaggcaa
    12721 cccacaagcg gtcgaattag tacaaaaatc tgcaaaagcg attgctaatt taattgcaga
    12781 tttaaaaatc agtttagata tacaaaaagt ggtgttaggt ggcagtgtgg gcttagcgga
    12841 agggtattta ccgcaggtgt ccaactattt agctgaaatg ccgagcgtat atcattgcat
    12901 attggaaaat gctctatccg ggcaagatgc cggcttaatc ggtgcggctt ggtgggcgga
    12961 aaaccaactt aatcaaggtt ttactcttta acaaattcta actccggaga tccaaatgaa
    13021 aaacttaaaa ggtattttta gtgcgttact cgtctctttc aacgaagacg gcaccatcaa
    13081 cgaaaaaggc ttacgccaaa ttatccgcca caacatcgac aaaatgaaag tggacggttt
    13141 atatgtcggc ggttcaaccg gtgaaaactt tatgctttct accgaagaga aaaaagaaat
    13201 tttccgcatt gccaaagacg aagcgaaaga tgaaatcgcc cttatcgccc aagtcggtag
    13261 cgtaaactta aaagaggcgg tagaactggg taaatatgcc acagaattag gctacgacag
    13321 cttatctgcg gtcacaccgt tctactacaa attcagcttc ccggaaatca aaaatttcta
    13381 cgaaaccatt attcgtgaaa ccggcaacaa aatgattgtt tactccattc cgttcttaac
    13441 cggcgtaaac atcggtgtga gccaattcgc tgaattattt gaaaacgaaa aaatcatcgg
    13501 tgtaaaattc actgccggcg atttctacct gttagagcgt ttacgcaaag cgttcccgaa
    13561 ccacttaatc tacgcaggtt tcgatgaaat gatgctaccg gcaacggtat taggtgtgga
    13621 cggtgtaatc ggctcaacct tcaacgtaaa cggtattcgt gcaagacaaa tctttgaatt
    13681 agcccaacaa ggcaaaatca aagaagcatt tgaaatccaa aatgtaacca acgatttaat
    13741 cgaaggcatt ttaggcaacg gtttatacca aaccatcaaa ggcttattgg agttagaagg
    13801 tgtgcaagca ggttactgcc gtgagccgat gacgaaagaa caaactccgg cacaaaaaga
    13861 agtgaccaaa cagttaaaag ctaaattctt gtaatttaag ttgcaaaagg ctaaacgaaa
    13921 atcaccgctt gtagtaacaa gcggtgattt tttctcaacg acttgcaatt aatgaagcca
    13981 ttctgctttt tctaccacgg tgtaacggtc accattatat tttaatcgca ctttttcttg
    14041 gcgagtattt tctgatttca actctctacc tatatcataa aaccctgtac tccagatatt
    14101 ttgagtaata tccgctttta caatgatatc attgaagccg ttggtctgtt tgttatccat
    14161 ttgcaatacc aagtttgtcg tttccgtttt ggtttcttta tcatctaaaa tagaccaaaa
    14221 agtcatcggg taatcaaaaa caagttttaa cttttctttt tccggcacaa ataacatcaa
    14281 atgatcattt gcagaatact caggagcact tggaccacga gcagcattat taaacctcac
    14341 gccgaaagct cttactttgt cgtttagaat ataacgggcg gtatcaattt taagactatc
    14401 tgcattcaca tcgatagagg catcgatctc catatcctgc tcccacgatt gcaatatcgc
    14461 attatcattc acacgagcaa caaaaagcgt ccacttaggt gtgttttcgt cgttttgata
    14521 attttctttg ctgtatttag gctctattac cgcataactc actaaggttt ccgttgaatt
    14581 gttaggagct gttttacagg cagaagctaa agtacgagca gaaagtttct cttgagaaat
    14641 cttaaagtaa tgagcaataa tttgatgttg ttgctcagca caaacctgtg tgttattacc
    14701 ttcatctgct tttatttgag ttgcaagcgt aagtaaaaga actaagagga gtatcttttt
    14761 catatagatt tatccgtata gaaagataac tcattctaaa agaaaagcag tctattctct
    14821 accacatttt gcaaaaagtg gcaaaaatag accgcttgta tggcatatca ctccgataaa
    14881 ctcaccccat aaaatggcgt gtagccaaac ggattttgca caaaaccttg cacccgtttg
    14941 ctgagtggta cataattgat agcgtgagcc agcggaataa atggcgtttg ttcgttggca
    15001 atttccaccg cttgttgata gagcttcgcc cgctcgtcac ggttttgggt ttgtacagct
    15061 tgagctaata actgctcaaa tttctcatcc gtccaacgcc cataatttgt tcccggaata
    15121 ttagatttgc ttaataaagg gaagaggaaa ttatcaggat caccattacg gcttgtccaa
    15181 ccataagtgc cggtagagaa ctcgccttca cgggtgcgtt tgttaaaatc cgcccattcg
    15241 tgggtcacta atttcgcttt caccccgatt ttcgcccaat ccgcctgaat aatttcagcc
    15301 gttcgacgtg ggtttgggtt agaagggcgc actaccggtt gtacccaaat ttccgtttca
    15361 aatccatcgg caaagccggc ttctgttaat agggctttgg ctttttcaag gttaaattca
    15421 tattgcggta aatttggatt atagcccaac accgaatcag ggaacggatt acttgccacc
    15481 gtgccaccgc cttgataaac agcctcgaca atcgctttct tatcggttgc ataatgcaag
    15541 gcttggcgaa ctttggcgtt atttaatgcc ggtttggtgc tgtttaagcc gatataagct
    15601 aaattcagcc cgtctctctg taacaaattc accttcggat cttgtttcat ttgggcaata
    15661 tcggaaatat tcgggaaatc aatcacatcg cattcacccg ctttcagttt agcataacga
    15721 gtgcccgcat caggggtaat actaaaaatc agtttctcaa attttgcttt accttggaaa
    15781 taatccgggt tcgcaacata acgcaccgct tgatcggttt gataggcttg gaacacaaaa
    15841 ggccctgtgc cgatcggggc ttgatccaat aactccggct tgccttccgc cagcaattta
    15901 tccgcatatt cttttgaata aatcgacaag aaatccatcg ctaaggttgt cagaaaagga
    15961 gcattaggtt gctgtaaggt gattttaaca gtgtaatcgt ccactttttc cactgttttt
    16021 aaaatcttcg gcaaattcat ccaatggtaa tagaaataag taccgtcaga gaccttatgg
    16081 taagggtggt ttaaatccat ctggcgttgg aacgagaaca ctacatcatc cgcattcagc
    16141 gttcgagttg gcgtaaattg tttattggaa tgaaatttca cgtctttacg taaatggaat
    16201 gtgtaactta agccatcgtc actgatttcc caacgttccg ccaaactcgg ctcaaccgtt
    16261 agttcaccgg ctttaaacgc taataagcga ttataaattt gttgtgaact ggcgttgaaa
    16321 tcattggcat cattagtaat tgcaggacta agtttcatcg gtggagttgc cacgcaatta
    16381 atcaacgtat ttaccggagc tgctgccacc aaactgctgg cacttaagag taaaacagaa
    16441 agagataatt gagaaagacg cataaagact ccttaacatt ttctactaac ttactcggca
    16501 ttaacgtatt ttcaaaataa ctaaaggtta taatttataa tcagaataat cagttgcaaa
    16561 ccaatgattc ttggctagaa tattggcagt tttttaaacg agagatttac tatgacatca
    16621 atttgtatca tcaccggcag caccttaggc ggtgcggaat atgttgccga tcacattaat
    16681 gaggtattaa gcgagcaagg ttttgaagtg gaactgttta accaagccac ccttgccgat
    16741 attgaaggta aagccaatct gattgtcgtt acctccaccc acggagccgg cgagctaccc
    16801 gaaaatatcc agccgttatt tgatgattta gcaaatagca cggcggattt cagctcaatg
    16861 aaatttggcg taatcggctt aggcagctca gattacgaca ccttctgcaa tgcagtaaat
    16921 attgttgaag aagctctcaa agcgaaaggg gcaagccaag tttgcgaatc actgagaatt
    16981 gatgtaatga ataatttcga ccacgatggc agtgctgaag aatggttacc atcatttatt
    17041 gaacaattat aacttaagta tcaatgaaaa aaatcagtgc aaataaggcg aagtggatta
    17101 aaattgctat tatcttaatc tatatgttca gccctgtaga tattctaccc gaagcaattt
    17161 taggcccact cggtttggtt gatgatgctg ccgcattaat gctattgatt aagactattt
    17221 tggaaaaata ggcatagtag acaaaatagt tgcaaaaaac caaaacaaaa cgaccgcttg
    17281 taacccaata caagtggtct tatcttttac ttaattcttt taagggagaa tatctcttga
    17341 aataggattt tgaagtgaaa taagcgtttc tgttgactga atatcatcaa tagactgaat
    17401 ttttcctgct aataccgaat gcaactcttc gatggtatgg gtcattactt taataaaaat
    17461 agaataatta ccagtggtat agtacgcctc aacaacttca ttaaattcac taagtttagc
    17521 aattacttta tcataatctt tggctgattt taaaataatg ccaataaagc aacacacgtc
    17581 atacccaacc ttacgctcgt ccactacgat tttggtagat tcaataatgc cggcttggcg
    17641 cattttttca acacggacat ggattgttgc ggcactcaca ccaaaaactt ttgccatttc
    17701 cgcataaggc gtgcgagcat ctttaattag ccaacgtaaa attttttgat ccagcttatc
    17761 aatttccgat ttagtcgtgt ttttactgta cattttttct cctcaaaatc aaactaatta
    17821 gataatattt aaaaatattg caaatatttt aaatgaaatt ta
//
LOCUS       DANKBV010000041        17733 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.041, whole genome shotgun sequence.
ACCESSION   DANKBV010000041 DANKBV010000000
VERSION     DANKBV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17733
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.041"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1214)
                     /gene="glyS"
                     /locus_tag="PWY43_001114"
     CDS             complement(<1..1214)
                     /gene="glyS"
                     /locus_tag="PWY43_001114"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2136000.1"
                     /translation="MTTQNFLAEIGTEELPPKALKKLATAFAENVENELNQAGLSFEK
                     VEWFAAPRRLAVKVLSLAESQPSKEVEKRGPAVSAAFDAEGKPTKAAEGWAKGCGITV
                     EQAERIATDKGEWLVHRAVIEGQPTKNLLVDIIAKSLANLPIPKTMRWGDKTEQFVRP
                     VHTVTLLFGAELIEGEILGVKSGRTIRGHRFLGEREFQIENADQYPQLLKDRGSVVAD
                     FNERKALILAKSQEKATALGGVADIEEDLLDEVTSLVEYPNVLTAKFEERFLAVPAEA
                     LVYTMKGDQKYFPIYSKDGKLLPHFIFVSNINPEDPSKIIEGNEKVVRPRLTDAEFFF
                     KTDLKQKLEDQLPRLETVLFQQQLGTLRDKTARIEALAGEIAKQIGADETKAKRAGLL
                     SKCDLMTNMVFE"
     gene            complement(1223..1687)
                     /locus_tag="PWY43_001115"
     CDS             complement(1223..1687)
                     /locus_tag="PWY43_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136001.1"
                     /translation="MSIKKLLSTTIDQLENIIQVSGESFEKITSDLAEKAKELNSEYN
                     IKCEKLGYAITLFEKNSEIEKRYISLLSDFDMDTSIKGFEKLSLLMNGKNDNARKEAE
                     EHFNELQNGLLEAQDYKKDLDIKIRKLARELYNENIAQLEIEEIKEHLYKNW"
     gene            complement(2065..3099)
                     /locus_tag="PWY43_001116"
     CDS             complement(2065..3099)
                     /locus_tag="PWY43_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL2136002.1"
                     /translation="MKTVIADNSAEFVNEISQLIQSSKQRMTVAVNAELTLLYWHIGK
                     RINDYILQGERAEYGQEVVKNLAQSLTEQFGKGWSKRHLNYTMQFATTFPNLEIVHAL
                     RAQLSWTHFKQIIHIDDPTKRGFYATMAAQERWSTRTLEDRIGSLLFERTAISKKPDE
                     TIIAELSLLREKGEYNQSMVLKDPYILDFLDLNDRYMERDLEDAILREIEQFLLELGA
                     GFTFVARQKRIQIDNDDFYIDLLFYNRKLKRLIAIELKTESFKHSHKSQMELYLAWLA
                     KYEQEADENPPLGMILCTGKKQEQVELLDLKNSDIHIAEYMTVLPSKVVLEQRLHLAV
                     ERAKERFLLK"
     gene            complement(3142..3402)
                     /locus_tag="PWY43_001117"
     CDS             complement(3142..3402)
                     /locus_tag="PWY43_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136003.1"
                     /translation="MKPILYFADWCPDTAPFVAELDRLGIEYEPCDITKGGSTLKPFL
                     KLRDTHSAFDHAKANGYIGIPALLIEGEKVVLDLAELEGIFG"
     gene            complement(3482..4390)
                     /gene="glyQ"
                     /locus_tag="PWY43_001118"
     CDS             complement(3482..4390)
                     /gene="glyQ"
                     /locus_tag="PWY43_001118"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2136004.1"
                     /translation="MSTKFNVKTFQGMILALQDYWASKGCTIVQPFDLEVGAGTSHPM
                     TCLRALGPEPMAFAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLDS
                     LKMLGFDPTQNDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPV
                     TGEITYGLERLAMYIQGVDSVYDLVWSDGELGKTTYGDVFHQNEVEQSTYNFEYADVD
                     FLFQCFEQYEKEAQYLLGLEKPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILR
                     IRALTKGVAEAYYASREALGFPGCKK"
     gene            4585..5196
                     /locus_tag="PWY43_001119"
     CDS             4585..5196
                     /locus_tag="PWY43_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentahemic C cytochrome"
                     /protein_id="HDL2136005.1"
                     /translation="MKSLSKIAFCFLAFIMPTFCFAGSNSYTTETIPLFADENLTQKI
                     GQLEAGAEIKLVQTTAKAAEIELNLWRKSKGFGRIWYHSFSKNITDAVFEKSFISNNP
                     LLEIVESKEDPNTGLVWQKVKMNVWVENAEFTQDLTNFWANTKNTYKTECSVCHKQRD
                     PKMHDANEWIAVFNGMVGFTDMDEATQKKVLRYLQMNAASSSQ"
     gene            complement(5280..5897)
                     /locus_tag="PWY43_001120"
     CDS             complement(5280..5897)
                     /locus_tag="PWY43_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2136006.1"
                     /translation="MILVCGANGLLGRAICDKLPREKTIAVLRKDAMDTFFSERNIAV
                     EIADLLDLESCNALLQKTQPEILITVVGGKNEQGIRSDKTANINLIKAVEKYSPTTKV
                     VLVTSVGCAEQWDLISPLAQQALGEALKAKTEAEAYLQQTALNWLIIRPGGLNNDEVN
                     DEYQIVRDLPNERKMYVSRKSVATGIVRLLDSTENHQIFSIINRG"
     gene            complement(5900..7717)
                     /locus_tag="PWY43_001121"
     CDS             complement(5900..7717)
                     /locus_tag="PWY43_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDL2136007.1"
                     /translation="MIIKDTHWTSQQTSPNAFPERQALMPIWGGKNIERNQWQSLWLE
                     QAPNALLQDGLAYFHIPFCASHCIFCGFYRNAWKENYSQIYTDRVISELRYESSLWRG
                     QGKIHAVYFGGGTPTALHTEDLVRLIRACYRYLPLAEDCEFTIEGRISHFDIDKAKAC
                     IEAGVNRISIGIQTFNSEIRKRLGRKHSGEEAQTYLKALSELNAVIVADLIFGLPNQT
                     DDIWQNDIEIATSLPISGLDIYAFNNYPFLPINRLIEHGKLPAPAGFDTQSLHYAYAV
                     EKLKAQGWQQVSNNHFAFPNRGERNRYNTLVKSNMPCLAFGSGAGGNFGGLSYQVQSD
                     LKTYLDSPLDQKSLSFLSKHGEHKALLGVIQHSMELGYLDTTPFEQNAKAEKLLQQWE
                     ALDLLKREKNQVYLNTSGRYWSPTLIRKLMLTLPQSSKDNNMITPLTKELENKLRTNL
                     KENPGQILEMLAGMNQCSLESVINCLPKEMVMQTGGERFVEIMQAIAGWNEAVTFIIH
                     TPDAIAEVTAKLPQGKIGRGFYNFEHGVDGGIHGHLRYENCAAIYLLDRPFMGKRTVA
                     LTFINQSGEAMFKIFVGRDEQGQLRENQIQAMRKLIGGC"
     gene            complement(7874..8563)
                     /locus_tag="PWY43_001122"
     CDS             complement(7874..8563)
                     /locus_tag="PWY43_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005577085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDL2136008.1"
                     /translation="MFMYLSHPLDPKDLAWPGEPVVEVTRCTDVGEETPFSSFITKLP
                     NHCGTHMDAPRHFVKDGLSINELPIEYFCHKEVALIEIPKGEAEGITKEDLLPYVDIL
                     SKVSFAFLRTGFEKYRTENPAIYQNECPYISTGAGQYLSDTFPNLKGVGIDFLALGSP
                     SSRVPDAENPKNCHRAILGYYTGRFTTVIEDMHLAELPKNANIKQFFNAPWRIKGLDS
                     SQVTCIVELQQ"
     gene            complement(8575..9915)
                     /locus_tag="PWY43_001123"
     CDS             complement(8575..9915)
                     /locus_tag="PWY43_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HDL2136009.1"
                     /translation="MLKKSLLCMLLISSALYAEDRVQENQLTNITLLAEITAQGQKIN
                     GVALEYPDNVLSGSDLRQLYQVKTALDSAEKENRSVLKAYVNNEPKVTALPQAGKFVI
                     IELDTADKNAATYSVKDANKAPMKFKSKDENGNIVETEKVQAVKIPEFFANRLIYSVE
                     QKGLLKLTSGTTLGKAEWTQTAEPDKIKTPYLDDFVAKQVSLDKPENKLNYRFYSPLQ
                     KNGEKYPLVIFLHGSGQVGSDNLAHLLSSKGAIATLPYAPSFVLAPQYDQVFDPFDNA
                     AKGQKGGIHWQTENRINLVLKMIDETLKANPQIDTTRIYINGLSRGAEGGLNLLLARP
                     NFFAGALLMSGREAYTNEWVDGNATKTNLAAIKHIPIWFFHSKEDKVSPVKGSRINYR
                     ILKDELNAPNVKYTEFTFEQAGDNGIVNNNPHNTWDAVYHSPEVIGWLLQQKRH"
     gene            complement(9977..11335)
                     /locus_tag="PWY43_001124"
     CDS             complement(9977..11335)
                     /locus_tag="PWY43_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntT protein"
                     /protein_id="HDL2136010.1"
                     /translation="MSNEMLILIGLASVVALLVIMIKGKVHPFLALGLVSIAVALTAG
                     IPMNKVIPTLISGMGSTLGSVALIVGLGAMLGKIIEKSHGADVLANWLLQRFGEKNAP
                     FALGMTGFIFGIPVFVDVGFIVLIPIIFSVARRLGGNILVYSLPIGLSMLLVHVLLPP
                     HPGIVAGAQNLNADIGFVLGIGLVAALPAYLIGQKFIPFFTKNYFVKVVTSTDLLQHQ
                     KDLIKEKGIELPSFGKVLAMISFPLILIMLGTVTATTLPKEHIVREVFSMVGSSPFAL
                     FVAVCLSAYVLGFRYGWTKHKLEEMLDSALAPIAGIILITGAGGMFGKVLDASGVGKA
                     LADVLANTGLPLLVLAFILSALLRAAQGSATVATITTTTILAPAAAAAGYTELQLALL
                     TCAIGAGSMTLSHVNDSLFWVWTKFFNVPVSVGLRTWTFLTTIFGVIAFAVVGIIWML
                     FV"
     gene            complement(11388..12164)
                     /locus_tag="PWY43_001125"
     CDS             complement(11388..12164)
                     /locus_tag="PWY43_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HDL2136011.1"
                     /translation="MPKFAANLTMMFNEVPFLDRFEAAANAGFKYVEYLWPYDYPAAE
                     LKAKLEQYGLKQVLFNTAAGDVAQGEWGVSAIPGREADSHRDIDLALEYALALGCKNV
                     HVMAAVVPEGEDREAYKQTFIENVQYAADKFKPHGINVLLEALSPEVKPNYLLKSQFD
                     TLEIVEAVNRDNVFVQLDYFHAQNVDGNLSRLTDKLKGKFAHIQIASVPDRHEPDEGE
                     INYQYIFNKLDEIGYEGYVGCEYKPRAETTSGLGWFEQYK"
     gene            complement(12166..12798)
                     /locus_tag="PWY43_001126"
     CDS             complement(12166..12798)
                     /locus_tag="PWY43_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDL2136012.1"
                     /translation="MTEQEQKIEMVNLARSLFERGYTVGGAGNLSVRLDENRILVTPT
                     GSSLGRLEADRLSVLDMQGNVLSGDKPSKESVFHLAMYQKNLACQAIVHLHSTYLTAL
                     SCLENLDTDNAMRAFTPYYVMRVGKLPVIPYYRPGDINIARELGERALDGKAFLLANH
                     GVVVTGSDLRDAVDNTEELEETAKLHFILQGQKVRYLTDDEVNDLKNRGK"
     gene            complement(12795..14036)
                     /locus_tag="PWY43_001127"
     CDS             complement(12795..14036)
                     /locus_tag="PWY43_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL2136013.1"
                     /translation="MLGIIADDFTGASDIASFLTENGLKTVQMNGVPKTTLNSQVDAI
                     VISLKSRSNPVEEAIQQSLQALDWLKQNGCSQFYFKYCSTFDSTEKGNIGPVTDALMA
                     ALDTDFTVVTPALPVNGRTIFNGYLFVGDVLLNESGMRNHPITPMKDANLVRLMDAQS
                     QGKTGLVAYADVIQGAERVKARFAELKQQGYKYAVVDAADNSQLAVLAEAVADFQLVT
                     GGSGLGAYMAAYRSGGKKGTNAFVPSKGKTVVLSGSCSVMTNTQVAAYQQKAPSYFLE
                     AEKAIQDPNYVETLYQWVIANLDGDFAPMVYATVPPEKLKEIQTQFGGDKASHAIENT
                     FAKLAEKLQAAGVTNFITAGGETSSIVVQKLGFSGFHIGKQIAPGVPWLQAVEKPIFL
                     ALKSGNFGKEDFFSFAQEMFV"
     gene            complement(14036..14941)
                     /locus_tag="PWY43_001128"
     CDS             complement(14036..14941)
                     /locus_tag="PWY43_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2136014.1"
                     /translation="MSQKYSVAVIGLGAMGMGAAKSCIAAGLDTYGVDLNPNYLETLK
                     AAGAKKVGTSAVDFAAELDAVLLLVVNANQVNAVLFGENGLAQHLKQGTVVMVSSTIS
                     AQGAKDISQKLTDLGLVMLDAPVSGGAAKAAQGEMTVMASGSSEAFEKLKPVLDAVAG
                     KVYNIGEEIGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTNAAGNS
                     WMFENRMKHVVDGDYTPLSMVDIFVKDLGLVNDTAKSLRFPLHLASTAYSMFTEASNA
                     GYGKEDDSAVIKIFSGIELPKKGDN"
     gene            15115..15873
                     /locus_tag="PWY43_001129"
     CDS             15115..15873
                     /locus_tag="PWY43_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2136015.1"
                     /translation="MIPLERQREILRLINNNGVASINFLAEQLKVSHMTIRRDIQKLE
                     EQGKVSLVSGGVQSLERLIMELPHDDKSMLFQEQKSAIGSLASQLIPSNTTIYLDAGT
                     TTLEIAHRIIDRNDLLVVTNDFVIAHYLSNSGKCDLIHIGGSVCKENLSTTGSLAAEF
                     LKNLSIDMAFISTSSWNLKGLTTPNENKIPVKKAILEASKQNILVTDSSKYGKVATFF
                     VCPLTVFDQIICDKGLFENVRDALKGLSIKLDLG"
     gene            complement(16013..17404)
                     /gene="ffh"
                     /locus_tag="PWY43_001130"
     CDS             complement(16013..17404)
                     /gene="ffh"
                     /locus_tag="PWY43_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL2136016.1"
                     /translation="MFENLSDRLSKTLRNITGKGRLTEDNIKDTLREVRMALLEADVA
                     LPVVRDFINKVKERALGVEVNKSLTPGQEFLKIVQSELETAMGEANEELNLAAQPPAV
                     ILMAGLQGAGKTTSVGKLAKFLKERHKKKVLVVSADVYRPAAIKQLQTLAETLKVDFF
                     PTETSQKPVAIAEAALKHAKLNFFDVLIVDTAGRLHVDGEMMEEIQQIHAVLNPIETL
                     FTVDAMTGQDAANTAKAFNEALPLTGVILTKVDGDARGGAALSIRQITGKPIKFLGVG
                     EKTDALEPFHPDRVASRILGMGDVMSLIEDIQRSVDQEKAEKIAAKFKKGDEFTLEDF
                     REQLIEMKKMGGMMSMLDKLPGAKNLPDHVKSQVDDKMFMKMEAIINSMTLKERANPD
                     IIKGSRRRRIALGSGTQVQDVNKLLKQFDDMQRMMKKMRKGGMAKMMRGVKGMMGGGL
                     GGLGGLGSMFGKR"
BASE COUNT         4963 a         3977 c         3451 g         5342 t
ORIGIN      
        1 aattcaaaca ccatattggt catcaggtca cattttgaga ggagacctgc acgtttcgct
       61 ttggtttcat ctgccccgat ttgcttcgca atttcacctg ccaacgcttc aatgcgagcg
      121 gttttatcac gcaatgtgcc aagctgttgc tggaacaaca cggtttcaag gcgtggcagt
      181 tggtcttcca acttctgttt taagtcggtt ttgaagaaga actccgcatc ggtcaaacgt
      241 gggcgaacca ctttttcgtt accctcgatg attttgcttg ggtcttccgg attgatgttc
      301 gacacaaaaa taaagtgcgg taacaatttg ccgtcttttg aataaatcgg gaagtatttt
      361 tggtcgcctt tcatggtgta aactaaggct tccgccggca cagcaaggaa acgctcttca
      421 aacttggcgg tgagtacgtt cgggtattcc accaacgagg tcacttcgtc cagcaaatct
      481 tcttcaatat cggcaacgcc acccaaagcg gttgcttttt cctgtgattt tgcaagaatt
      541 aaggctttac gctcattgaa atctgctacc acagatccac gatctttcaa cagttgcgga
      601 tattgatcgg cgttctcaat ttggaactca cgttcaccta agaaacggtg accacgaata
      661 gtacggccgc ttttcacgcc taagatctca ccttcgatca actccgcccc gaataaaagt
      721 gtcacggtat gcacagggcg gacgaactgc tcggttttat cgccccaacg catcgttttt
      781 ggaatcggca agttcgccag cgatttggcg ataatatcga ccaacagatt tttagtcggc
      841 tgaccttcaa tcacggcacg atgcaccaac cattcgcctt tgtcggtggc aatacgctca
      901 gcttgctcta cggtaatccc gcagcccttc gcccagcctt ccgctgcctt agtcggcttg
      961 ccttcggcat caaacgctgc cgacaccgcc ggccctcgtt tctcaacctc tttactcggc
     1021 tggctttccg caaggctcaa caccttcacc gccaaacggc gaggagcagc aaaccattcc
     1081 actttctcaa acgaaagacc tgcttggttt aattcgtttt ctacattctc cgcaaatgcg
     1141 gtcgctaatt ttttgagtgc cttcggtggc agctcttctg tgccgatctc ggcaaggaag
     1201 ttttgtgttg tcattgttta aattaccaat ttttatataa atgttctttg atttcttcta
     1261 tttctaattg tgctatattt tcattataaa gctctctagc taatttccta attttgatgt
     1321 ctaaatcttt tttataatct tgagcttcta atagcccatt ttgtaattca ttaaaatgtt
     1381 cttcagcttc ttttctagca ttatcattct tgccattcat tagtagagac aatttttcaa
     1441 atccttttat acttgtatcc atatcaaagt cagataataa tgatatgtag cgtttttcta
     1501 tttccgagtt tttttcaaat aatgttatgg cgtatcccaa tttttcacat ttaatattat
     1561 attctgaatt taattctttt gctttttctg ctaagtcact tgtaattttt tcgaaacttt
     1621 cgcccgatac ttgtattata ttctcaagtt gatctatagt tgtagataat aattttttaa
     1681 ttgacatata tttcctttat tatatccttt tctttgcttt tattgtactt atcaagaaat
     1741 gagagcaact ttatgattaa cttgcaaaaa agtactagac taatcaatct ctcactacgt
     1801 tttcacttct gctaaatctc ccctagcccc tctttgctaa aaaggggaat ttgatcttcg
     1861 taaatttata attcatcttt cttcattaag attgtaaata gtcagatgaa ttaaaattac
     1921 tcactaaatg atcgccccct ctttagccaa agagggggag gggggagatt tgaaaatctc
     1981 taaccaccca ccaacggaat atgcaaccaa atcgtttcca tcacactttc attttgcaac
     2041 atttccgctg attttaaccg cttgctattt tagtaaaaac cgctctttcg ccctttccac
     2101 cgccaaatgc aaccgttgtt ccaataccac tttactcggc aacacggtca tatattccgc
     2161 aatatgaata tcggaatttt tcaaatccaa caattccact tgctcctgct ttttaccggt
     2221 gcagagaatc attccaagcg gtgggttttc gtctgcttct tgctcgtatt tcgccagcca
     2281 cgccaaatag agttccattt gacttttgtg ggaatgtttg aagctttcgg tttttagttc
     2341 aatcgcaatc aggcgtttga gtttacggtt ataaaacagc agatcgatat agaaatcatc
     2401 attatcaatc tgaatccttt tttggcgggc gacaaaggta aaacccgccc caagttccag
     2461 taaaaactgt tcgatctctc gtagaatcgc atcttcgaga tcacgttcca tataacggtc
     2521 gttaaggtcg agaaaatcca aaatataggg atctttcaac accattgact gattatattc
     2581 gcctttttca cgtagcaaac tcaattcagc gataatggtt tcatcgggct ttttggaaat
     2641 tgccgtgcgt tcaaacaata atgagccaat gcgatcttct aatgtccttg tactccaacg
     2701 ctcctgtgca gccattgttg catagaaccc tcgctttgtt ggatcatcaa tatgaataat
     2761 ctgcttaaaa tgtgtccagc ttaattgtgc acgcagtgcg tgcacaattt ccaaattagg
     2821 gaaagttgta gcaaattgca ttgtataatt taaatggcgt ttactccaac ctttaccaaa
     2881 ttgctccgtc agactttgag caagattttt caccacttct tgcccatatt ccgcacgttc
     2941 accctgcaaa atatagtcat tgatccgttt tccgatatgc caataaagca aggttaattc
     3001 tgcattaacc gcaacggtca ttcgttgctt actcgattga ataagttgtg aaatttcatt
     3061 cacaaattca gccgaattat ctgctattac tgtcttcatt attggctcct ttgcaacatt
     3121 tccgccaaat tcaaccgctt gttagccgaa aataccctct aattcagcca aatccagcac
     3181 gactttttcg ccttcgatca gcaatgccgg aatgccaata tagccgttgg ctttggcgtg
     3241 gtcaaaggca gaatgcgtgt ctcgcagttt caaaaatggt ttaagtgttg agcctccttt
     3301 ggtgatgtcg cacggctcat attcaatgcc caagcggtct agttctgcca caaatggggc
     3361 ggtatccgga caccaatcgg cgaagtataa aatcggtttc atcgtaaatc cttgtaggga
     3421 cacgatgctt cgtgtccgtt ttgtaattta ttgacggacg ccaagcatgg cgtccctacg
     3481 attatttttt acaccccggg aaccccaacg cttcacggct ggcatagtac gcttcagcaa
     3541 cgcctttggt taaggcacga atgcgtagaa tgtagcgttg gcgttcagtt accgagatcg
     3601 ctttacgagc atctaataag ttaaagctgt gagcggcttt taaaatgcgt tcgtatgccg
     3661 gcaatggcaa tggtttttct aggcctaaca ggtattgggc ttctttttcg tactgctcga
     3721 agcattggaa caggaagtcc acatcagcgt attcaaagtt gtaggtggat tgctccactt
     3781 cgttttggtg gaatacgtcg ccgtaggtgg ttttaccgag ttcgccgtca gaccacacga
     3841 ggtcgtacac gctgtccacg ccttgaatgt acatcgccaa acgctctaag ccgtaggtga
     3901 tttcgcctgt caccggttta cattccaagc cgccgacttg ctggaagtag gtgaattggg
     3961 tcacttccat tccgttcagc cacacttccc aacctaagcc ccacgcccca agcgttgggt
     4021 tttcccagtt gtcttccaca aaacgaatgt cgttttgggt tgggtcgaaa ccgagcattt
     4081 tcaggctgtc gaggtaaagt tcttgaatgt tatccggcga aggtttaatc accacttgga
     4141 actggtagta gtgctgtaaa cggttcggat tttcgccgta gcgaccgtct gtcgggcggc
     4201 gagatggttg cacataggca aacgccattg gctcagggcc aagcgcacgc aggcaggtca
     4261 ttgggtgcga ggtgcctgcc cccacttcta aatcgaacgg ctgcacaatg gtgcaacctt
     4321 tgcttgccca gtagtcttgc agggctaaaa tcattccttg aaaggttttt acgttgaatt
     4381 ttgtactcat tttattttct cttgaatttg aaaaatggtc gaaaaatact ggttattata
     4441 gcttgtaagc ggtcattttt tgggattttt ttgcaaaaaa ttaagaaatc gacataaaat
     4501 cagttttatg atgtaaatca acgctttttt gctcaatttg tgtaacatat aacctaattt
     4561 ctcatctaat ttgtggggtt tatgatgaaa tcattatcga aaatcgcttt ctgtttcttg
     4621 gcattcatca tgccaacatt ttgttttgcc ggttcaaaca gttacaccac ggaaacaatc
     4681 cctttatttg ccgatgaaaa tttgacacag aaaatcgggc agctagaagc cggagcagaa
     4741 attaaattgg tgcaaacaac cgcaaaagcc gctgaaattg agctgaattt atggcgaaaa
     4801 agcaaaggtt tcgggcggat ttggtatcat tctttcagta aaaatattac cgatgcggta
     4861 tttgagaaat cttttatcag caacaaccca ctgttagaga ttgtagagag caaggaagat
     4921 ccgaacaccg gcttggtatg gcaaaaagtg aaaatgaacg tgtgggtgga aaatgccgaa
     4981 tttacccaag atctcactaa cttttgggcg aacaccaaaa atacctataa aacagaatgc
     5041 agcgtgtgcc ataaacaacg tgatcctaaa atgcatgatg caaacgaatg gattgcagta
     5101 tttaacggaa tggtcggttt taccgatatg gacgaagcta cccagaaaaa ggtgcttcgc
     5161 tacttgcaga tgaatgcggc atccagttct caataaaata gaaaaagatc tcacaagcgg
     5221 tctgttttat agttgcaaaa taaccataaa atatgcccgc ttgccttcgt tgaaatatac
     5281 tatcccctat taataataga gaagatttga tgattttccg tactatccag taaacgcact
     5341 atgcctgttg cgacagattt acggcttaca tacattttcc gttcattagg taaatcacgc
     5401 acaatctgat actcatcatt tacttcatca ttatttaacc caccgggtcg aataatcagc
     5461 caatttaatg ctgtttgctg taaataagct tcagcttccg tcttggcttt tagtgcttca
     5521 cctaaagctt gctgtgctaa tgggctaatt aaatcccatt gttctgcaca cccaacacta
     5581 gtaactaaaa ctactttcgt agttggtgaa tatttttcaa ctgcttttat caaattaata
     5641 tttgctgttt tgtcgctacg aataccttgt tcattttttc ctcctactac cgtaatcaag
     5701 atttccggtt gtgttttttg taacaaggcg ttgcaggatt ccaaatctaa taaatctgct
     5761 atttctactg ctatatttcg ttcgctgaaa aacgtatcca tagcatcttt tcgtaagacg
     5821 gctatcgttt tctctctagg taatttgtca cagattgctc gtcctaacaa cccatttgcc
     5881 ccacaaacca aaatcatgac tagcatcctc caattaattt ccgcatggct tgaatctgat
     5941 tttctcgtaa ttgaccttgt tcatcacgcc caacaaaaat cttaaacatc gcctcaccgc
     6001 tttgattgat aaaagttaac gccaccgttc tttttcccat aaatgggcga tcaagtaagt
     6061 aaatagcagc acaattttca tagcgtaaat gaccgtgaat tcctccatct acaccatgtt
     6121 caaaattata aaagcctcgc ccaattttac cttgtggcaa tttagcagtt acctctgcaa
     6181 tcgcatcagg cgtatgaata ataaaagtca cagcttcatt ccaaccagca attgcctgca
     6241 taatttccac aaaacgctca ccacctgttt gcattaccat ttctttgggc agacagttaa
     6301 ttaccgattc caagctacat tgattcattc ctgccagcat ttctaaaatt tgccctgggt
     6361 tttcttttaa atttgtccgc aatttatttt ctaattcttt agttaatggc gttatcatgt
     6421 tgttatcctt acttgattga ggaagtgtta acattagttt acgaattaat gttggtgacc
     6481 agtaacgacc actggtattg agatatactt gatttttttc acgcttcagt aaatcaagcg
     6541 cttcccattg ttgcaataat ttttcagctt tcgcattttg ttcaaaaggc gttgtatcta
     6601 agtaacccaa ttccatgcta tgttgtatca caccaagcag tgctttatgt tcaccatgtt
     6661 tactcaaaaa agaaaggctt ttctgatcaa gaggcgaatc taagtaggtc tttaaatcag
     6721 attgtacttg gtaactcagc ccaccaaaat ttccccccgc accggaacca aatgccaaac
     6781 aaggcatatt cgatttaacc aaagtattat agcgattgcg ttcacctcga tttggaaaag
     6841 caaagtggtt attgctcact tgctgccaac cttgtgcttt taacttctct actgcataag
     6901 catagtgtaa gctttgggtg tcaaaccctg cgggcgcagg taattttcca tgttcaatta
     6961 agcgattaat aggcaggaaa ggataattat taaaggcgta aatatctaag cctgagatcg
     7021 gtaaactagt cgcaatttca atatcgtttt gccagatatc atctgtttgg tttggcaagc
     7081 caaaaattaa atcagcaaca atcacagcat ttaattccga taaggctttt aaataagttt
     7141 gagcctcttc gccactatgt ttgcgcccta aacgtttgcg aatttcggag ttaaaagtct
     7201 gaataccaat agaaatacgg ttcacgcctg cttcaataca agccttagcc ttatctatat
     7261 caaaatggct aattctaccc tcaatagtaa attcacagtc ttcagcgagt ggcaaataac
     7321 gataacaagc tcgaatcaat cgtactaaat cttctgtatg taacgctgtt ggcgttcctc
     7381 caccaaaata aacggcatga atcttacctt gtcctcgcca tagtgaagat tcatatcgaa
     7441 gttcactaat cacacgatca gtataaatct ggctataatt ttccttccaa gcattgcgat
     7501 aaaaaccaca aaaaatacaa tgactagcac aaaacggaat atggaaataa gctaaaccat
     7561 cctgtaataa cgcatttggt gcttgttcca gccataaaga ttgccattga ttgcgttcaa
     7621 tatttttccc accccaaata ggcataagtg cttgtcgttc tgggaaagca tttggagagg
     7681 tttgttgaga tgtccagtgt gtatctttta taatcattat cgaaacaaaa tagtgctata
     7741 aacaaggtca tatttataac aaaattaagt tatgaatgac ttgattaaaa tcaaataata
     7801 attattttca attaatgatt gagttatgct tactatagta aatagcctct cctaaaatga
     7861 atatagctat ggtttattgc tgtaattcca caatacaagt cacttgactg gaatctaacc
     7921 ctttaattcg ccacggtgca ttaaaaaact gcttaatgtt ggcattttta gggagttccg
     7981 ctaagtgcat atcttcaatc acagtggtaa acctgccggt atagtagcct aaaatcgctc
     8041 gatggcaatt tttagggttt tccgcatcag gcactcggga agaaggagac cctagtgcca
     8101 agaaatcaat gcctactcct ttcaaattcg ggaaagtatc agataaatac tgcccggcac
     8161 cggttgaaat ataggggcat tcattttgat aaatagcagg gttctcggtt ctatatttct
     8221 caaaccccgt tcgtaaaaag gcaaaagaca ccttggagag aatatcgaca taaggcaata
     8281 aatcttcttt ggtgatccct tctgcctctc ctttgggaat ttcaattaaa gcgacctctt
     8341 tatggcagaa atactctatc ggcaattcat tgatacttaa accgtctttc acaaagtggc
     8401 gaggagcgtc catatgtgtg ccgcaatgat tcggtagttt cgtgataaaa ctgctaaatg
     8461 gcgtttcttc acctacgtct gtgcagcgag tcacctcaac aacaggttct cccggccaag
     8521 ctaaatcttt aggatctaac gggtgagaca aatacataaa catagtgagc ctccttagtg
     8581 gcgtttttgc tgaagtaacc acccaatcac ctcagggctg tgatacacag cgtcccaagt
     8641 attatgaggg ttattattga caatgccgtt gtcgccggct tgttcaaaag tgaattcggt
     8701 atatttcaca ttgggggcat ttaactcatc tttgagaatg cggtagttaa tgcgagaacc
     8761 ttttaccggc gagactttgt cctctttact atggaaaaac caaatcggta tatgtttaat
     8821 tgccgccaaa ttcgttttgg tggcattgcc gtccacccat tcgttggtgt aggcttcacg
     8881 accgctcatt aataatgctc cggcaaagaa attcgggcga gccaacaaca gatttaagcc
     8941 cccttctgca cctcgagata aaccattgat ataaatgcgt gtggtatcaa tttgaggatt
     9001 ggctttgagg gtttcatcaa tcatttttaa cacgagattg atacgatttt ccgtttgcca
     9061 gtgaataccg cctttttgcc ccttggcggc attatcaaat gggtcaaaca cttggtcgta
     9121 ttgaggagca agcacaaaac ttggggcata aggcagggta gcaatcgccc ctttactgga
     9181 aagcaggtga gccaaattat ccgatccgac ttgccctgag ccgtgtagga aaatcacaag
     9241 cgggtatttt tcaccatttt tttgcaaagg cgaatagaag cggtagttca gcttattttc
     9301 aggtttgtct aagctcactt gttttgcgac aaaatcatcg agataagggg ttttgatttt
     9361 gtcgggttca gcggtttgtg tccattcagc cttgcctaga gttgtgccgc tggttaattt
     9421 aagcaagcct ttttgttcca ccgaatagat taggcgattt gcaaaaaatt cgggaatttt
     9481 gaccgcttgt actttctcgg tttcgacaat gttgccgttt tcatctttgg atttaaactt
     9541 catcggtgct ttattggcat cttttacgct ataagttgcc gcatttttat ctgccgtatc
     9601 caattcaata attacaaatt taccggcttg cggaagtgcg gtcacttttg gctcattatt
     9661 gacataggct ttgagtaccg aacggttttc tttctccgca ctatctaatg ccgttttgac
     9721 ctgatacagt tggcgaagat cgctacccga taatacatta tcaggatatt ccaatgccac
     9781 gccgttaatt ttttgacctt gagcggtgat ttcggcaagc agcgtgatat tggttaattg
     9841 attttcttga actctatcct ccgcataaag ggcggaagag attaatagca tacataaaag
     9901 cgattttttc agcataggtt tctcctaaaa aaatccacct cataagaggc ggattttaag
     9961 cagtaacaga gtgatattaa acgaataaca tccaaataat gccaacaaca gcaaaggcga
    10021 ttacaccgaa aatagtggtt aaaaacgtcc aagttcttaa gcctaccgat acgggaacat
    10081 tgaagaactt cgtccatacc cagaagagtg aatcattaac gtgggataag gtcattgaac
    10141 cagctccgat agcacaagtg agcaatgcaa gctgtaattc agtgtacccc gctgctgcgg
    10201 ctgccggagc taaaatcgtt gtagtagtaa tggttgcaac tgttgccgag ccttgtgcgg
    10261 cacgcagtaa agcagacaga atgaaagcca gaaccagtaa tggtaaacct gtgttggcta
    10321 atacatctgc caatgcttta cctacgccac tggcatctaa cactttaccg aacataccgc
    10381 cggcaccagt gattaaaata atcccggcaa tcggtgctaa cgcagaatcc agcatttctt
    10441 ctaatttatg cttagtccaa ccgtatctaa agcctagtac ataagcagaa agacaaacag
    10501 ccacaaataa ggcaaacgga gagcttccta ccatactgaa cacttcacgc acgatatgtt
    10561 ctttcggcaa ggttgttgcc gttaccgtac ctaacataat caggatcaaa gggaaagaaa
    10621 tcattgccaa tactttaccg aaagaaggta attcaatccc tttctcttta atcaaatctt
    10681 tttggtgctg taaaagatcg gttgaagtca ctacttttac aaagtaattt ttcgtaaaga
    10741 atggaataaa cttctgaccg attaaatatg ccggcagtgc tgcaactaaa ccaattccca
    10801 atacaaagcc aatatcagcg tttaagttct gtgcacccgc cacaattccc gggtgaggcg
    10861 gaagcaaaac gtgaaccaat aacatagaaa gcccgatagg tagtgagtaa accaaaatat
    10921 ttccacctaa acgtctcgcc acactaaaaa taatcgggat caatacgata aaaccaacat
    10981 caacgaaaac cggtatcccg aagataaagc cagtcatacc caatgcaaag ggagcatttt
    11041 tctcaccaaa acgctgtaac aaccaatttg ctaatacgtc cgcaccgtga gatttttcga
    11101 taattttccc taacattgct cctaaaccaa cgatcagagc aacacttcct agagtactcc
    11161 ccatccctga aatcaaggtt gggattactt tattcattgg aatacctgca gttaaggcaa
    11221 ctgcgatact tactaatccc aatgctaaga atgggtgtac tttaccttta atcataatga
    11281 ccagaagagc aactacgctt gctaatccaa tcaaaattaa catttcattt gacataaagt
    11341 ttccttctaa atgttgtatt cacgtattta tccgctaatc atcgtggtta tttatactgc
    11401 tcaaaccaac ctagtcctga ggtggtttcc gctcgtggtt tatactcgca acctacatag
    11461 ccttcatacc cgatttcatc gagtttgttg aagatatatt ggtagttaat ttccccttcg
    11521 tccggttcgt ggcgatctgg cacagaggca atttgaatgt gggcaaattt acccttgagt
    11581 ttgtcggtta ggcgagataa gttgccgtcc acattctgtg cgtggaagta gtcaagctgt
    11641 acaaacacat tgtctcggtt taccgcttcc acaatttcta gcgtatcaaa ctggctttta
    11701 agcagataat tcggtttcac ttccgggctt agggcttcaa gcaaaacgtt aatgccgtgc
    11761 ggtttgaatt tatcggcggc atattgcacg ttttcgataa aggtttgttt ataggcttca
    11821 cgatcttcgc cttctggcac gaccgctgcc atcacgtgca catttttaca acctaatgcg
    11881 agggcgtatt ccaacgccaa atcaatgtca cgatggctat cggcttcacg ccccggaatg
    11941 gcggaaacgc cccattcccc ttgagcaaca tcgccggctg cggtattgaa taacacctgt
    12001 tttaagccat attgctcaag tttggctttc agttctgctg ccggataatc gtaaggccat
    12061 aaatactcta catatttaaa tccagcgttg gcagccgctt caaaacgatc taaaaacggc
    12121 acttcgttaa acatcatcgt caggtttgct gcaaatttag gcattttatt ttcctctgtt
    12181 tttcagatca ttgacttcgt catcggtgag ataacggact ttttgacctt gtagaataaa
    12241 atggagtttg gcggtttctt ccaactcttc ggtattatcc accgcatcac gcaggtctga
    12301 acctgtaact acaaccccgt gatttgccag taaaaaggct ttaccgtcca aggctcgttc
    12361 ccccaactct cgggcaatgt tgatgtcgcc tgggcggtag taaggaatga ccggcaattt
    12421 tcccacccgc atcacatagt aaggggtgaa agcacgcata gcgttgtcgg tatccaaatt
    12481 ctccaagcaa gaaagtgcgg ttaaataagt ggaatgtaga tgcacaatcg cttggcaagc
    12541 caggtttttc tgatacatcg ctaagtggaa tactgattct tttgaaggct tatctcctga
    12601 aagaacatta ccctgcatat ccaatacgga aaggcggtct gcctctaaac gcccgagcga
    12661 tgagccggtt ggggtaacta aaatgcgatt ttcatctaaa cgcacagaga ggttgcccgc
    12721 tccgccaacg gtataacccc gctcaaacag cgagcgggca agattgacca tttcaatctt
    12781 ttgttcttgt tctgtcatac aaacatctcc tgagcaaaac tgaagaaatc ttctttaccg
    12841 aagttacccg atttcaatgc aagaaaaatc ggtttttcga ccgcttgtaa ccacggcacg
    12901 cccggtgcaa tttgtttgcc gatgtggaaa ccgctgaatc caagtttctg aaccacaata
    12961 ctcgaagttt caccgcctgc ggtaataaag ttagttacgc ctgcggcttg taatttttcg
    13021 gctaatttgg caaaggtatt ttcaatggcg tggctggctt tatcgccgcc gaattgggtt
    13081 tgaatctctt tcaatttttc agggggaacg gtggcataaa ccattggggc aaagtcaccg
    13141 tctaggtttg caatcaccca ttgataaagc gtttctacat agttcggatc ctgaatggct
    13201 ttttccgctt ctaagaaata acttggggct ttttgctggt aagccgccac ttgggtattg
    13261 gtcattaccg agcaagaacc cgaaagcaca acggttttgc ctttgcttgg tacaaacgca
    13321 ttcgtgcctt ttttgccgcc tgaacggtaa gccgccatat aagcccctaa gcctgaaccg
    13381 cctgtgacta attgaaaatc ggcaaccgct tcggcaagca ccgcaagttg tgaattatcg
    13441 gcagcatcta ctaccgcata tttgtagcct tgctgtttca attcagcaaa acgggctttc
    13501 acacgctctg ccccttgaat cacatcagca taggcaacca agccggtttt gccttgagat
    13561 tgtgcatcca ttaaacgcac caagttggca tctttcatcg gggtaatcgg gtggttacgc
    13621 ataccagatt cattcagtag cacatcgccc acaaacaaat agccgttgaa aatggttctg
    13681 ccattgacag gcagagccgg tgtgactacg gtaaaatcgg tatctaatgc cgccattagg
    13741 gcatcggtta ccggcccgat attgcctttc tccgtgctgt caaaagtgga acaatattta
    13801 aagtagaatt gcgagcaacc attttgtttc agccaatcta aggcttgtaa tgattgttgg
    13861 attgcttctt ccacagggtt agagcgagat ttcaggctaa tcacaatcgc atcaacttga
    13921 ctgttaagtg tagttttcgg cacaccattc atttgcaccg tttttagccc gttttcggtt
    13981 aagaagctgg caatatcgct cgcaccagta aaatcatctg caataatgcc taacattagt
    14041 tgtctccctt ttttggtagc tcaataccgc tgaaaatttt gatgaccgca ctatcatctt
    14101 ctttgccgta acctgcattg cttgcttcag taaacattga gtacgctgtg cttgctaaat
    14161 gaagtgggaa gcgtaatgat ttcgctgtgt cattcactaa gcctaaatct ttgacgaaaa
    14221 tatctaccat tgaaagcggg gtgtagtcgc catcaacaac gtgtttcata cggttttcaa
    14281 acatccaaga gttacctgct gcattagtta ccacatcgta catcagatct aacggaatgc
    14341 cggcacgagc ggctaatgcc atcgcttctg cacctgcggc aatatgcaca ccagctaata
    14401 attggtggat aattttcacc gttgcaccta agccgatctc ttcgccgatg ttataaactt
    14461 ttcctgcaac agcatctaaa acaggtttta gtttctcaaa cgcttcactt gagcctgatg
    14521 ccattacggt catttcacct tgtgccgctt ttgctgcccc accggaaacc ggagcatcta
    14581 acatcaccaa gcctaaatct gttaatttct gtgaaatatc ttttgcacct tgtgcagaaa
    14641 tggtggaaga caccatcacc accgtgcctt gtttcaaatg ttgggctaag ccgttttcac
    14701 cgaataacac agcattcact tggttggcat taaccaccaa taacaacacc gcatctaatt
    14761 cagcggcaaa atcgaccgca cttgtgccga ctttcttagc acctgctgct tttaaggttt
    14821 ccagataatt cgggttaaga tccacaccgt atgtgtctaa tccggcagcg atacaagatt
    14881 ttgctgctcc catacccatt gctcctaagc cgatgacggc tacgctatat ttttgactca
    14941 ttgtttattc cctcaaataa attaatgaac ttgatggcgg tatattaacc aatagaaaag
    15001 ataaaatctg tgatgaagat cacaaatatt aacaataaat gtgaattttt gtgattttaa
    15061 ttttggaagc taaatctgat ataatctctt ttaacaatta aaataggtta aaaaatgatc
    15121 cccttagaga gacaacgaga aatactacga cttattaata ataatggtgt cgcaagtatt
    15181 aattttctgg cagaacagct aaaagtatca catatgacaa ttcgccgtga tattcaaaag
    15241 ctggaagagc aaggtaaggt atccctagta tctggtggag ttcaatctct tgaacgatta
    15301 atcatggaat taccacacga tgataaatca atgttattcc aagagcagaa atcagctata
    15361 ggatctttag catcacagct aattccctca aacacaacta tttatcttga tgctggaact
    15421 actacattag aaatagctca tcggataatc gatagaaatg acttactagt tgtaacaaac
    15481 gattttgtta ttgctcacta tcttagtaat tcaggaaaat gtgatttaat tcatatagga
    15541 ggaagtgtct gtaaagaaaa cctatcgaca actggttcac ttgcagcaga atttctgaaa
    15601 aacctatcaa tcgatatggc atttatatct acttcatcct ggaatttaaa aggtctaaca
    15661 acgccgaacg aaaataaaat ccccgttaaa aaagcaattt tagaggcatc aaagcaaaac
    15721 attttagtga cagactcttc aaaatatgga aaagtcgcaa ctttttttgt ttgccctctt
    15781 actgtatttg accagattat ttgtgataaa ggattatttg agaatgtacg agacgcactg
    15841 aaagggctaa gtattaaatt agatttagga taatcttctt accaattttt tctgagaacg
    15901 acaagcaatc aaaaagtatt atttttgcaa ataagatatt aatagggaaa gttaacaatc
    15961 ctagcaacgg attgttaact gattttcggc tggtactaat aaaataggaa tattaacgtt
    16021 taccaaacat actgcctaag ccaccgagac cgcctagacc accacccatc atacctttaa
    16081 caccacgcat catttttgcc attccgcctt tacgcatttt tttcatcata cgttgcatat
    16141 catcaaactg cttgagcagc ttatttacat cttgcacctg tgttccggag ccaagtgcga
    16201 tacggcggcg gcgtgagcct ttaataatat ccggatttgc ccgctctttt aaggtcatag
    16261 aattgatgat ggcttccatt ttcatgaaca ttttatcatc aacctgactt ttaacgtggt
    16321 caggcaggtt tttcgccccc ggtagtttat cgagcatcga catcattcca cccatttttt
    16381 tcatttcgat aagctgctca cggaaatctt ccaaggtaaa ttcatcgcct tttttgaatt
    16441 tggcggcaat tttttccgct ttttcttgat caaccgagcg ttgaatatct tcaattaaag
    16501 acattacatc gcccatacct aaaatgcggg aagccacacg gtcagggtgg aacggctcaa
    16561 gggcatcagt tttttcaccc acacctaaga atttaatcgg tttaccggtg atttggcgaa
    16621 tagaaagtgc cgcaccgcct cgggcatcac catcgacttt ggtaagaata acaccggtaa
    16681 gcggcaacgc ttcgttaaag gcttttgcgg tgttggcggc gtcttgaccg gtcatcgcat
    16741 caaccgtaaa tagcgtttca atcggattca gcacagcgtg gatttgttgg atctcttcca
    16801 tcatttcgcc atcaacgtgt aaacgaccgg cagtatccac aatcaataca tcaaagaaat
    16861 taagcttagc gtgcttcagt gccgcttctg caatcgctac cggtttttgg ctggtttcgg
    16921 tcgggaagaa gtccactttt aacgtttcag caagggtctg tagctgcttg atagcggcag
    16981 ggcggtaaac atcggcagaa actaccaaca cctttttctt gtggcgttct ttcaggaatt
    17041 ttgccaattt gccgacagag gtggttttac ccgcaccttg caaacctgcc attaaaatta
    17101 ccgcaggcgg ttgggccgca agattcagct cctcgttggc ttctcccatt gccgtttcta
    17161 attcgctttg tacgattttt aagaactctt gccccggcgt gaggcttttg tttacctcaa
    17221 cccctaacgc acgctctttg accttgttga tgaaatcacg cacaacaggg agggcaacat
    17281 ccgcctccaa caatgccata cgaacttcac gcagggtatc tttaatatta tcttccgtta
    17341 ggcgaccttt gcccgtaatg ttacgcagcg ttttagacag tctgtcagag aggttttcaa
    17401 acatatcagc tttctcaatt tgcaaaattt taggaaaaaa gtaccgcttg tgagcggaaa
    17461 gttgccgcca ttatacaaaa aaaagcgtta cggttatagg gaaataaaaa aacggcatag
    17521 cgattaaact atgccgtaaa atttgacgat attcgaggaa tcattattta gttatattgt
    17581 gcgatcataa tactccgaat tgcgtataaa agtgaagtga tattttcaaa tttatgttag
    17641 aaatgagagg taaattacaa aattaaaggg gatattattg tttgaaaaaa taagagcgaa
    17701 aagaaaaccc tgatgattgc tcatcagggc ttc
//
LOCUS       DANKBV010000042        16839 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.042, whole genome shotgun sequence.
ACCESSION   DANKBV010000042 DANKBV010000000
VERSION     DANKBV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16839
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.042"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(38..754)
                     /locus_tag="PWY43_001131"
     CDS             complement(38..754)
                     /locus_tag="PWY43_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL2136017.1"
                     /translation="MPVSISNQMKLDLAKLEQNAMLDLYEVDLRTLKDKSGNAGSVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEASGFERNSSGPSNRPTLTLSNLFGLITGIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNAKIPSRLIMADTCTWIYRSSECGYTGKQYFDEKDKATSDPKADKCSHCLNG
                     CTLRGNQRNFGGFVSVNKLG"
     gene            complement(754..1083)
                     /locus_tag="PWY43_001132"
     CDS             complement(754..1083)
                     /locus_tag="PWY43_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2136018.1"
                     /translation="METLQWCVRTDLAVENTPEIMEVKFGDGYTQRSPKGLNNLLRAY
                     TGTIKVKKGEHLAVEAFFAKHRGVSPFYFKDPYTQQNKKVVCSSWPAKMVGLNHWEFS
                     VTFKEVP"
     gene            complement(1083..4634)
                     /locus_tag="PWY43_001133"
     CDS             complement(1083..4634)
                     /locus_tag="PWY43_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2136019.1"
                     /translation="MSSSNRLTITLDVENSKLLSGFAQSDTVANKFARSFILNMDQAQ
                     QKARQFADRSTKYLKNIEQAANNLNSSVSKERFWQIANRLQFPTSKVISYANSYTELG
                     NKLKLVTESEAQQARAMAAVYDISLKTAQSTQATSSVYQTFAQNAKQLGISQNDVAKL
                     TETVAKSVAISGASSATASNALVQFSQSLLMGKLKAQEFNSLMTQTPSVIQAIAKGLG
                     ITTAELKAMVDNGEMSAEKMIEGLKKAESYVNGQYAKTTTTISGAMQNLSTAAEKWVG
                     EMDSTVGASQKAVAVLNALNAHFDTFAKTALVAGGAFVLFKGYMSVRPFIEARAAIYE
                     NRKAIIAENQARQQTIATRQQELVVLSQQTALKLEQTLQTYNQNKAELEAIAVKQQKI
                     TKEREEIAVAIQAAATRTERLALSAELQALDQKEIALGQQKIAVERQQIATKQALKVA
                     YAENAVAQSAMNAGMVTATTTTRAMNGVLAAARAELNLLKAAALSNPLMTLAMVATTA
                     GTALWGFFESQKAAREEALRYADSLDSVRANIEKMTKAQTDAEMVKLSRSIKEQETAL
                     ASLKQRQAELTREIEQGATVLDDAFGAGVRFEKSAKELAKAQEELKLVTADVEKAQNT
                     LNASLEAQEALQAHVPIANLREEFVKLYPHIDESQIKVDGLNIAIGNFTVKSPEMVIA
                     ANNIANALGGVAGEAMRAAILVANLSSMKLDANGAAIIDPKHLEKIEQIERNNAIASA
                     KGKDKIALQVKDALIKSGMKEGDAGYERLKAAYEQQFTLQNAPKRGGKPKKTKAERSA
                     ESYQNQVAEMTNRLSGLKIDKEDLVKYGGISQYQEVRKLTEDIALNAEKYKNYGTEGV
                     AKLKELAAKIDSEQQLLDIEQFQVDHDKKLEAMQFELDLLGKTRTEKELLQFNNQLDL
                     EAERLKINMTKENIALLDQKIAKIKEEYELHQKKQEETRASVMMGIKEGWNNIETDVS
                     NVAANVANITQNTFDGMADSLTNFVMTGKADFRSFANSILSDLSKMIIKMMLFNAIKQ
                     GATMLFGYQGPTTTVNGNAVYGLGTWATGGYTGNGGKYTPAGIVHKGEYVITKEATSR
                     LGLDYLNYLNYGRRGFASGGGVAVPRVPSSSYQPKSAQSSISVQVINNGEPVDAKVSQ
                     KQQGEQTQITVELMRKIARQEANGMIQNNFRAGGVFA"
     gene            complement(4688..4915)
                     /locus_tag="PWY43_001134"
     CDS             complement(4688..4915)
                     /locus_tag="PWY43_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136020.1"
                     /translation="MFEALDRFMINTIKIAIIVFLVACIFISMPNWWIASILVLFPTA
                     YLLDKYWWRNTEMYRRKMDKLVKEMKNNRQI"
     gene            complement(4978..5208)
                     /locus_tag="PWY43_001135"
     CDS             complement(4978..5208)
                     /locus_tag="PWY43_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL2136021.1"
                     /translation="MPERHFAEYQLFYQEQPFGLWREDYRTAQLAHLTAMINRDPKGK
                     APELAEFMPFFNRSEDVEEMEDNGVADYLAKR"
     gene            complement(5253..5654)
                     /locus_tag="PWY43_001136"
     CDS             complement(5253..5654)
                     /locus_tag="PWY43_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136022.1"
                     /translation="MTLREKLLANKPKLQPIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGALLFNPLNIDDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSS"
     gene            complement(5737..6378)
                     /locus_tag="PWY43_001137"
     CDS             complement(5737..6378)
                     /locus_tag="PWY43_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136023.1"
                     /translation="MAKTTKVQGTKFRISVGREAQKAITAINLNTATLTIASSGYKKG
                     DAIEITGCGQLDGIYPVLSVTGEQVKLCEEVKWEGKDLPTSYTKAKAALVQFSEQFCA
                     VKNIEKSDDTLNTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPTTEMQARLRELFFN
                     KSTFAYKLELPDNHGTTYGVGFIESGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            complement(6406..6798)
                     /locus_tag="PWY43_001138"
     CDS             complement(6406..6798)
                     /locus_tag="PWY43_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2136024.1"
                     /translation="MKIHTKIRKEVLELLQTKLPDIENFYNGLPTFIDIEEQQLAVSV
                     SLDDISLQEMTVCNDQWSAKLNITIHLKSVENAEDELDEWAEKIREIIDYSDFEYLET
                     ISLSQYQYEQDQNQRTWYTATLIFDVEY"
     gene            complement(6795..7319)
                     /locus_tag="PWY43_001139"
     CDS             complement(6795..7319)
                     /locus_tag="PWY43_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2136025.1"
                     /translation="MGSKVTGLKELQASLKKIARQTVPKAAAQAIRTVGRQAMNKAVK
                     SVAAEIGVNQKTIKGRAKMTAKPTPSRLQATIKVNRTHMPMIRILERKSNRLSVSKGS
                     IRVGKHTVQRGFRQRLANGRTHIMFRQGRKRYGIDVAKVPLSQPLTQAFEQELKKYPE
                     QVQAELAKQLAGKL"
     gene            complement(7323..7625)
                     /locus_tag="PWY43_001140"
     CDS             complement(7323..7625)
                     /locus_tag="PWY43_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2136026.1"
                     /translation="MSSPFEQAMAAADQTIQHTMMSEWLIGGKPYPATYDEAPAIFNG
                     LHSSDDRAIHGTERTLTLFRASGYKPRLDDRVSGNGKKYLVKSYHFVDQLIVLQLE"
     gene            complement(7618..7941)
                     /locus_tag="PWY43_001141"
     CDS             complement(7618..7941)
                     /locus_tag="PWY43_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDL2136027.1"
                     /translation="MAKNYIQDGNTVRLTTTKAVTSGDVIVTEDLIAIAVTDAAKNEA
                     VVGLTTGVFSVKAKQADDIKQGAVLYWSEADGATITAGSNKRLGIAWKASGTSSATVD
                     VKINV"
     gene            complement(7954..8115)
                     /locus_tag="PWY43_001142"
     CDS             complement(7954..8115)
                     /locus_tag="PWY43_001142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136028.1"
                     /translation="MQYNIAREVRKMLELMNKSTKANVLIFGIWAMGLLFALSAFISA
                     MTNFISILH"
     gene            complement(8194..10155)
                     /locus_tag="PWY43_001143"
     CDS             complement(8194..10155)
                     /locus_tag="PWY43_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2136029.1"
                     /translation="MKWYSIQAKANDTAEISIFDEIGFWGVTAQQFAKDLKALGNNLK
                     QINLHIHSPGGDVFDGIAIYNLLKNHPANKTVYIDGLAASMASVIAMAGSEIIMPENA
                     MMMIHKPWGIQGGDADDMRKYADLLDKVESTLVTAYSSKTGKSTDELAEMLKEETWLN
                     ARECIELGFADKLANPLVAMASIQSKKLEDYTKMPNEIKAMLFTPQGNAGAATPTPEQ
                     PQPPAQAVENPQNVTKPDAMAVLAQRNAAIKATFAAFGTQFDGLLAECLADVSMTAEQ
                     AKDKLLAKLGENTTPSVPQNHIHAGNGNIVGDSVKQSLMARAGQDTDKTNARDNAYNA
                     MTLRELARASLVDRGVGISGYTPMQMVGLAFTHSSSDFGQILIDVAHKSLIKGWETAT
                     ENYEQFTTRGILTDFRPAKRVGLGEFGYLPEVGEGEEYTYGTIGDEGAQVALATYGQL
                     FSITRQAIINDDMHLLTKIPEKMGQAAKATIAKLVFALITGNAVAQDGKKLFDSSHKN
                     TLANAKLDVEHIDKAIQLMNGFVNSRGEPLSIEPEFMLLPTSLHTKAKQILGSSSVEG
                     ADINSGIINPIRDIVSPIKSPRLQIADPKSWYLLNKEAIEVSYLDGVDSPYIEQQHGF
                     TVDGVSTKVRIDAGVNVIDYRGVVKVTNA"
     gene            complement(10559..11755)
                     /locus_tag="PWY43_001144"
     CDS             complement(10559..11755)
                     /locus_tag="PWY43_001144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136030.1"
                     /translation="MTTVVLDTVKVVANPPVIKPIFPPEFSEMEKLKRENYYFGRRIA
                     YAPNKVQSYVSLANSSVELSKKINELEKKIISAKSIGEIFKIISGLLMLYAKILNKVE
                     QEKLKKLYSLLVSQYDEVNKIKKAYLSKKEIAEKAEKSNLKKFALTKVNPRDYDLILS
                     VSGGSRLWWLHVGKHKADEVNEAQKLIDALIILNETLTEFSNESHRLVTTLSRHIVTN
                     IKQTPKLPITSRNRNTPVTSFDMLYYYRAKTGKSVTLKQLGLFNTVRELVKKPNALGK
                     KDNRSVHGDFIQQIITKNARSFKNTYSFKEGIWNINNALWAIGSAIVEGNFTGSAVQK
                     GDRFYLEGSIDYSFYDKFTDPYDIFNIIPGEWNPDGTSYDIKDNWRENVSIPIDERAY
                     KNLNKR"
     gene            complement(11759..13270)
                     /locus_tag="PWY43_001145"
     CDS             complement(11759..13270)
                     /locus_tag="PWY43_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2136031.1"
                     /translation="MNFLEKTIATLSPKWAANRSRNRYVLNAYEAAQPSRTHKASRES
                     KGANSTVRQSAVSLREQARALDQNHDIVIGILDKMEERVIGSKGIHIEPQPLTLAGDV
                     HEDLAEQIRKLWAEWSVKPEVTGLYTRPLLERMLLRTWLRDGEVFVQLVKGKVAGLEH
                     GSPIAFSLEALEPDFVPMQSDEAKNGLVQGVFLNAWRKPTAYQVYLDNPQESNGMYGK
                     VKTVLAENMLHLAFRKRLHQIRGVSMLHGVIVRLADLKEYEESERVAARIAAAMTMYI
                     KKGDAALYDDDSNSDGNRLFDIAPGAVIDDLKPGEDIGLINSNRPNVNLETFRNGQLR
                     ATAAGTRSSYSSIARDYNGTYSAQRQELVESFEGYAVLQDAFVAAISRPIYREWLKMA
                     IASQAIKIPSDIDERSLFNAVYSGPVMPWIDPIKEANAWKERIKGGLATEGQAIRASG
                     NNPAEVKRQRIVEIRENQAEGLKFDTDLTNYSQQNSGSSNRNLEDSTKENEKE"
     gene            complement(13270..13494)
                     /locus_tag="PWY43_001146"
     CDS             complement(13270..13494)
                     /locus_tag="PWY43_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136032.1"
                     /translation="MSLYTIDELKDKIRTLDEKIETAQSQVSFNGRSVSYQVAELTKQ
                     RDRYQTMLDEQLAQSGQHTKQHRIKYARFI"
     gene            complement(13491..15602)
                     /locus_tag="PWY43_001147"
     CDS             complement(13491..15602)
                     /locus_tag="PWY43_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDL2136033.1"
                     /translation="MFASAKEIRRDMANAVKVPRRMKVSDAVAEYMRVPLGGGNSVKW
                     DKHTAAYMLEPMDCLNSREYDAVIFVGPARTGKTIGLIDGWITYSIICDPSDFLLVQL
                     TQEKASEHSRKRLDRTFRCSPEIAKRLSPRKNDNNVHDKYFRAGNLLKIGWPSINVLS
                     SSDYKYVALTDYDRWPEDVDGEGDGFSLASKRTTTFMSAGMTLVESSPGKDIVDLKHI
                     PKSTHEAPPTTGILSLYNRGDRRRFYWQCPCCSEYFEPSMANMVGYREESDFVKASEN
                     ARLQCPHCQALIPPELKRELNIKGVWLKEGQKIDASGQISGESRKSRIASFWLEGPAA
                     AYQTWAQLTYKLLNAEHEYEMTGSEETLKAVTNTDWGLPYLPRSALEQRRSDELMERR
                     EEVEEKTVPPQCRFIVAAVDVQGGKNRRFVVQMVGYGENGERWLIDRYNISHTLPDRD
                     GVIEKIDPRIPEDWHILISDVLEKRYVLAHKPSDFMPILAMAVDSGGEEGVTDNAYQF
                     WRKCRRDGYAKRVYLVKGDSTKRQKLITKTHPDNTSRSDRHASARGDVPLYLLQTDYL
                     KDRINNALSRDTVGANYIHFPDWLGEWFFNELTYEERGADGKWRKPGKGNNEAFDLFC
                     YAHAIAILRGYERIKWGDENDVPSWAKLPDINPNIIRETPRTAEQAVEIEEKPAKPQP
                     KPTKAKSSWLSGGGRKTGGWL"
     gene            complement(15602..16081)
                     /locus_tag="PWY43_001148"
     CDS             complement(15602..16081)
                     /locus_tag="PWY43_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDL2136034.1"
                     /translation="MDNLFDLKLNINQIAELTGLHRQTVSQRVAGLTPSLGSNSKLKL
                     YALRDLILTGLAEKMSADVDSLSPVDRKSFWQAENERLKYEEKTGELIPASEVAFEMG
                     AMAKAVVQTLETLPDILERDCGLQPKDLIRVQQVIDDVRDQMALHIQQAPTTEQGDD"
     gene            complement(16351..16584)
                     /locus_tag="PWY43_001149"
     CDS             complement(16351..16584)
                     /locus_tag="PWY43_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136035.1"
                     /translation="MVLLIALSGCTSKTIVSTEYLYPPAAYLVPCERTAFSGKTYGDT
                     VDYLIKVMGERDLCASQIDRIREWQAQTKQGFK"
     gene            complement(16550..16822)
                     /locus_tag="PWY43_001150"
     CDS             complement(16550..16822)
                     /locus_tag="PWY43_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 family protein"
                     /protein_id="HDL2136036.1"
                     /translation="MWHQSKQIDGLRAENQTQAQTIEQQQKANQRLTDSLEQERQAVE
                     KSQKIANELRNKVEVAKNEITSILAQDSCAKADLPNGVADSIKRLH"
BASE COUNT         4120 a         3888 c         3519 g         5312 t
ORIGIN      
        1 gagcgtagtc gattatttgt tgctccaatt ccatttctta ccccaattta tttacagaca
       61 cgaatccacc aaaattgcgt tgattacctc gcaatgtaca accattcaag caatgactgc
      121 atttatcggc ttttggatct gacgtcgctt tatctttttc atcaaaatat tgcttaccgg
      181 tataaccaca ttctgacgag cggtaaatcc aagtgcaggt gtctgccata atcagtcggc
      241 tgggtatttt ggcattatcg gtttcaatcg gtaatgccag cgtaaaggtc gccgtttctc
      301 gggtgaggct ggtaagctgc tcaatcacaa aatgactgat tatttcctga tttggatcag
      361 ctttcgcatt acccccggca aaattcacgg catctaaata ttgggcgtaa acctgatgcc
      421 ggcggacaat cgcaccaata cattcgtcaa actgattggc aatgccggta attaaaccaa
      481 acagatttga tagcgtgaga gtcgggcgat tgctcgggcc gctactgttt cgttcaaatc
      541 cacttgcctc aatcgggtac ggatcgtaag tccgcccttg ccagacaata cttgttttga
      601 gttcatttaa ccccgaataa aagcggtaaa ctgagccagc attaccgctt ttatccttta
      661 atgtgcgtaa atctacctcg tacaaatcaa gcattgcatt ttgctcaagt ttggcaaggt
      721 cgagtttcat ttgattggat atgctaacag gcattagggt acttccttaa aggttacact
      781 aaattcccaa tggtttaagc caaccatttt tgcgggccaa ctgctacaca ctaccttttt
      841 gttttgttgt gtgtacggat ctttaaagta aaaaggggaa acaccacgat gtttggcaaa
      901 aaatgcctcc accgctaaat gttccccctt tttcactttg attgtgcctg tataggctcg
      961 taataaattg tttaagcctt tgggcgagcg ttgggtataa ccatcgccga atttgacttc
     1021 cattatttca ggggtatttt caaccgctaa atcggtgcga acacaccatt gtaatgtttc
     1081 cattaggcaa atactcctcc ggctctaaag ttgttctgaa tcattccgtt cgcttcctga
     1141 cgggcaattt tacgcattaa ctcaacagta atttgcgttt gctcgccttg ctgtttttgg
     1201 ctgactttgg catctaccgg ctcaccatta ttgatgacct gtacgctgat actactttga
     1261 gccgatttcg gctgataaga tgatgacggt actcttggta cagcgacacc accgccagag
     1321 gcaaaaccac gtctgccata gttaagataa ttcaaataat ccaaaccaag acgagaggtt
     1381 gcctctttgg tgatgacgta ttcccctttg tggacgatac ccgctggggt gtatttgccg
     1441 ccattgcctg tgtagccacc tgttgcccaa gtacctaatc cataaacagc attaccattt
     1501 accgttgtag tcggtccttg gtagccaaac aacatagttg caccttgttt gatagcatta
     1561 aataacatca ttttgataat cattttgcta agatcagata atatggaatt agcaaaacta
     1621 cgaaaatccg ctttacccgt catcacaaaa ttagtcaagc tatccgccat accgtcaaag
     1681 gtattttgag taatattggc aacatttgcc gccacattgc tgacatcggt ttcaatattg
     1741 ttccagcctt cttttattcc catcattaca gaagcacgag tttcttcctg ctttttctga
     1801 tgtagctcgt attcttcctt aattttggca attttctgat ctaaaagagc aatattctct
     1861 ttcgtcatat taattttcag ccgctcggcc tctaaatcta attgattgtt aaattgtaac
     1921 aattcttttt cagtacgagt ttttcccagt aaatccagct cgaattgcat tgcctctagc
     1981 tttttatcgt gatcgacttg gaattgctca atgtctaaaa gctgttgttc agaatcaatt
     2041 ttagcagcca gctctttgag tttagccacg ccttcagtgc cgtagttttt atatttttca
     2101 gcattcagag caatatcttc agtaagttta cgcacttcct gatattgaga aatgccacca
     2161 tattttacga gatcttcttt atctatcttt aagccggata aacgattggt catctccgct
     2221 acttggtttt ggtaactttc ggcagagcgt tcggctttgg ttttcttcgg ttttccgccg
     2281 cgcttagggg cattttgcaa cgtaaattgt tgctcatatg ctgcttttag tcgctcgtat
     2341 ccggcatcgc cctctttcat acctgattta atcagagcgt cttttacttg caatgcgatt
     2401 ttatctttgc ctttagccga agcaatcgca ttgttacgtt caatttgctc gattttttca
     2461 agatgtttag gatcgataat tgcagcaccg ttagcatcca gcttcatact tgacagatta
     2521 gcgactagaa tagccgctct catagcctct ccggcaacgc caccaagagc attagcaatg
     2581 ttattagcgg caataaccat ctccggtgat ttgacggtaa aattaccgat agcaatattt
     2641 aagccatcaa ctttgatttg gctttcgtca atgtgaggat ataatttgac aaattcctct
     2701 cgtaggttag cgattggaac gtgagcttgt aaggcttctt gagcttctaa actggcattt
     2761 aatgtatttt gtgctttttc aacatcagca gtaactaact ttaactcttc ttgtgctttc
     2821 gctaactctt tcgcactttt ttcgaaacgg acaccggcac caaaggcatc atctaaaacc
     2881 gtagctcctt gctcgatttc tcgtgtaagt tcagcttggc gttgtttcag actagctaat
     2941 gctgtttcct gttctttgat agaacgagac aatttaacca tttctgcatc agtttgagcc
     3001 ttagtcattt tttcgatatt ggctcgaacg gagtctaagc tgtccgcgta gcggagggct
     3061 tcttcacgag ctgctttttg actttcaaaa aatccccaaa gggcagtacc ggcagtcgtt
     3121 gccaccattg caagagtcat taatgggtta gacagtgcag ctgctttcag taagttcaat
     3181 tcagctcttg cagccgctaa aaccccattc atcgctctgg ttgttgtggt tgcagtgacc
     3241 atacctgcat tcattgcact ttgagcaact gcattttcag catacgccac tttgagagcc
     3301 tgttttgttg caatttgctg gcgttcaacc gctattttct gttgcccgag agcgatttct
     3361 ttctgatcta gcgcttgcag ttcagcagat aacgctaatc tttccgtacg agttgccgcc
     3421 gcttgaatag caacagcaat ttcttctcgt tcttttgtga ttttctgctg tttaaccgca
     3481 atcgcctcta attcggcttt attttgattg taggtttgta atgtttgttc cagttttaag
     3541 gcggtttgtt gagagagtac aaccaattcc tgctgacgag tagctatcgt ttgttggcga
     3601 gcctgatttt ccgcaataat tgcttttcga ttctcataaa tcgccgctct agcctcgata
     3661 aatgggcgta cactcatata gcctttgaaa agcacaaatg caccacctgc gacaagtgct
     3721 gttttagcga acgtatcaaa atgagcattt aacgcattca gaacggcaac ggctttctgt
     3781 gacgcaccaa cggtactatc catttcgcca acccactttt cagccgcagt tgaaagattt
     3841 tgcattgctc cactaattgt agtggttgtt ttggcgtact gtccgtttac ataactctct
     3901 gcttttttta gcccctcaat cattttttca gcagacatct caccgttatc caccattgct
     3961 ttaagttcgg cagtggtaat gcctaaccct ttggcaatcg cctgaataac agacggtgtt
     4021 tgggtcatca atgagttaaa ttcttgggct ttgagcttac ccattaacaa cgattggctg
     4081 aattgcacta gggcgtttga agcggttgcc gaacttgcac cggaaatagc aaccgatttg
     4141 gctaccgttt ccgttagttt tgccacatcg ttttgagaaa taccgagctg ttttgcattt
     4201 tgagcaaagg tctgataaac cgaagaggtt gcctgtgtag attgggcggt tttgagcgaa
     4261 atatcataca ccgccgccat tgctcgggct tgttgggctt cactttcggt gactaatttg
     4321 agtttattac cgagttcggt gtagctattg gcataactaa tcactttact tgtcgggaat
     4381 tgcaaccgat ttgcaatttg ccaaaaacgt tctttggata cagagctatt aagattattt
     4441 gctgcttgtt caatattttt tagatatttt gtcgacctat cagcaaattg acgtgctttt
     4501 tgctgagcct gatccatatt caaaatgaag ctacgagcaa acttatttgc tactgtatca
     4561 gattgtgcga atccagatag taacttagaa ttttctacat ctaaggtaat agttaatctg
     4621 tttgagctac tcactttttc accccaaata aaaagcccgc ataaagcggg ctagaattga
     4681 tgttaattta gatttgacga ttatttttca tctctttaac taatttatcc attttgcggc
     4741 gatacatttc ggtatttcgc caccagtatt tatcaagcaa ataggcagtc gggaaaagta
     4801 ctaagataga agctatccac cagttcggca tactaataaa gatgcaagcg accaaaaata
     4861 caataattgc aattttaatc gtattaatca taaaacggtc tagtgcttca aacatacttc
     4921 cctccttctc tttgaggaaa tatatatcca agagcaagga atttcaagct gttttcttta
     4981 ccttttcgcc aaataatctg ccacaccatt atcttccatt tcctccacat cctcactccg
     5041 attaaaaaac ggcataaatt ctgcaagttc aggggctttg cctttcggat cacggttgat
     5101 cattgcggtt aagtgggcaa gttgggcggt gcggtaatct tcccgccaca agccgaaagg
     5161 ctgttcttgg tagaaaagtt gatattcggc aaagtggcgt tcgggcattt gttcgatttc
     5221 ttcaagggtt ttgctcagtg cgagcgaaag ggttagctgg aactttcttc gctcgctgag
     5281 tcttttgggg cggacacggc ttggttaaag tcgataatca ctttactgtc taattcggca
     5341 atcgcattga gatcgtcaat gttaagtggg ttaaacagta atgcaccgtt ttcgtcacat
     5401 aagcggtagg caaccgattg agcaaggcgg tatttttcgc caaaacggtt gagagcctca
     5461 tcaaaggttt catcatcttc tgccggtaat tcaacatttt cttgttctgc ttgttgaatg
     5521 agccagcttc gggtttcaaa aatctgctta ttcatatcgc caacagtggc ttcacgtagg
     5581 tagtaggtct caccgttgat ttcgataggt tgtaatttag gtttgttggc aaggagtttc
     5641 tcacgcagtg tcattgttcg ttccttttgt aaaattctga tataaaaaga ccgcttgtta
     5701 tttctacaag cggtcgaatt ttttgatttc ttgcaattaa actaacaagt agtcacgttt
     5761 gcttggctta atagatacag agccttcgta tttgcctttg acttcaccgc taaaaccatt
     5821 gccggattcg ataaagccta caccgtaagt tgtgccgtgg ttatccggta attccagttt
     5881 ataggcaaag gtggatttgt tgaaaaacag ttcgcgtaag cgggcttgca tttctgtagt
     5941 tggcttgtgg aaaaagctca atttaattga gccaaactca atttcacccg gctcagtttc
     6001 tgtgccttcc gagcaaaccg ttgtaacatc ttctgtattt aaggtgtcat cggatttttc
     6061 gatattttta accgcacaga actgctcgga gaactgtacc aaagcagctt tcgctttagt
     6121 ataactagtc ggtaagtctt taccttccca ttttacttct tcgcaaagtt taacttgttc
     6181 gcctgtcacg gacagcacag gataaatccc gtctaactga ccgcaaccgg taatttcgat
     6241 ggcatcgcct tttttgtagc ctgatgatgc aatcgttaga gtagcagtat ttaagttaat
     6301 tgcggtaatt gctttttgag cctcacgtcc aacgctaatt ctaaatttcg taccttggac
     6361 ttttgtcgtt tttgccattg taattctcct attgaaattg tttgtttaat attcaacatc
     6421 aaagattaac gtcgctgtgt accatgttct ttggttttgg tcttgttcat actgatattg
     6481 agagagtgag atggtctcta aatactcaaa gtcggaatag tcgataattt cgcgaatttt
     6541 ttccgcccac tcatctaatt catcttctgc attctcgaca gatttcaaat ggattgtaat
     6601 gttgagcttg gcactccatt gatcattaca aacagtcatt tcttgcagac tgatgtcatc
     6661 gagagaaacc gacactgcaa gttgctgctc ctcaatatca ataaaagttg gcagcccgtt
     6721 ataaaaattc tcaatatccg gtaatttggt ctgtaataat tcaaggactt cttttcggat
     6781 tttagtgtgg attttcataa tttccccgcg agttgcttgg ctaattcagc ttgcacttgt
     6841 tctgggtatt ttttcaactc ttgctcaaat gcttgcgtaa gtggttggga tagcggaact
     6901 tttgctacat caatgccgta gcgtttacga ccttgacgaa acataatgtg cgttctcccg
     6961 ttcgcaagcc gttggcgaaa gccccgttgc accgtatgtt ttcctacccg aatactgcct
     7021 ttgcttactg acaagcgatt cgattttcgc tctaaaatgc ggatcatcgg catatgggta
     7081 cgattgactt taatcgtggc ttgcagtcgg gacggtgtgg gttttgccgt catttttgct
     7141 ctgcctttga ttgtcttttg atttacgcca atctctgccg ccaccgattt aaccgcctta
     7201 ttcattgctt gccgcccaac agtacggatt gcttgagcgg ctgctttagg tacggtttgc
     7261 ctagctattt ttttcaggga ggcttgcagt tcttttaaac cggtcacttt agaacccatt
     7321 gtttactcca attgcaatac gattaactga tcgacaaagt gataggattt aacaaggtat
     7381 ttcttgccat ttccgctcac tcggtcgtcc aatctcggtt tgtagccact cgcccgaaat
     7441 agggttagcg tgcgttccgt gccgtggata gctcggtcat cgcttgagtg caagccatta
     7501 aaaatcgctg gtgcctcatc gtatgtcgca gggtagggct taccgccaat caaccattcg
     7561 ctcatcattg tgtgttggat ggtttgatcg gcggctgcca ttgcctgctc aaacgggcta
     7621 gacattgatt ttcacatcaa ctgttgcaga tgatgtgcct gaggctttcc aggcaatgcc
     7681 taagcgtttg tttgagccag ccgtgatcgt tgccccgtct gcttccgacc aatacagcac
     7741 cgcaccttgt ttgatgtcgt ctgcttgctt cgctttcaca ctaaatacac cggtggttaa
     7801 accgactacc gcctcgtttt ttgccgcatc agttactgca atcgcaatta aatcttcggt
     7861 gacaatcaca tcaccggagg ttacggcttt ggtggtggtt aaacgcaccg tattgccgtc
     7921 ttgaatatag tttttagcca taattaggtt cctttaatga agaatagaaa taaaattggt
     7981 cattgcacta ataaacgccg ataaagcaaa aagtagcccc atcgcccaaa taccgaaaat
     8041 caacacattg gctttggtag atttattcat tagttctaac attttgcgta cctctcttgc
     8101 tatgttatac tgcatttaat ttaatccttg actgataagg gttaaaacaa aaccccgagt
     8161 tattccgcta actcggggtt tattattttg caattaagcg ttggtcactt tcaccacgcc
     8221 acggtagtca atcacattga caccggcatc aatacggact ttggtcgaaa caccgtccac
     8281 cgtaaagccg tgctgttgct cgatgtaagg gctatccacc ccgtccagat aagagacttc
     8341 aatcgcctct ttattgagca agtaccatga tttcggatct gcaatttgta agcgaggcga
     8401 tttaatcggg ctgacaatat cacggatcgg gttaatgata ccgctgttga tgtctgcacc
     8461 ttccacgctt gacgaaccaa gaatctgttt tgctttggta tgcaatgagg ttggtaacag
     8521 cataaattct ggctcaatag agagcggttc accacggctg tttacaaagc cattcattaa
     8581 ctggattgcc ttgtcgatat gctcaacatc taacttcgca ttcgcaagcg tatttttatg
     8641 gctgctatca aacagttttt ttccgtcttg ggcgacagcg ttgccggtga ttaaggcaaa
     8701 caccaatttg gcgatagtgg cttttgccgc ttgccccatt ttttccggga ttttggttaa
     8761 aaggtgcata tcgtcattga tgatcgcctg acgggtaatg ctaaataatt gcccgtaggt
     8821 cgccaatgcc acttgcgccc cttcatcgcc gatggtgccg taggtatatt cttccccttc
     8881 gcccacttcc ggcaggtaac caaactcacc caatccaacc cgttttgccg gtcggaagtc
     8941 agtcagaatg ccacgagtgg taaactgctc gtaattctct gtcgccgttt cccagccctt
     9001 aatcaaggat ttgtgcgcca catcaatcag aatttgccca aagtccgaac tcgaatgggt
     9061 aaaggcaaga ccgaccatct gcattggcgt ataaccgcta atccccacgc cacgatcgac
     9121 caatgaggca cgagccaatt cacgcaaggt cattgcattg taggcattat ctcttgcgtt
     9181 ggttttgtcg gtatcttgac cagcacgagc cattaacgac tgcttcacgc tgtcgccaac
     9241 gatgttaccg ttacctgcgt gaatgtggtt ttgcggtacg cttggggtgg tgttttcgcc
     9301 cagtttggca aggagcttgt ctttggcttg ctcagcggtc attgacacat cagctaaaca
     9361 ttctgccagc aagccgtcaa attgtgtgcc gaaagcggca aaggtcgctt tgatcgccgc
     9421 attacgttga gctaataccg ccatcgcatc gggttttgtc acattttgtg gattttcgac
     9481 cgcttgcgct gggggttggg gttgttcagg agttggggtt gcagcccccg cattgccttg
     9541 tggcgtaaat aacattgctt tgatttcgtt tggcattttt gtatagtcct ctaatttttt
     9601 agattgaata gacgccatcg ccacaagggg attggcgagt ttatcagcaa aacccaactc
     9661 aatgcactca cgggcattaa gccaagtctc ttctttcagc atttctgcta attcatcggt
     9721 agatttacca gttttcgagc tgtacgccgt aactagcgtg ctttccactt tatcaagcaa
     9781 atccgcatat ttccgcatat catcggcatc gccgccttga atgccccacg gtttgtggat
     9841 catcatcata gcgttttctg gcataatgat ttcgctgcct gccattgcaa ttacgctagc
     9901 catagatgca gcaagaccgt caatatagac ggtcttgtta gcagggtgat tttttagcag
     9961 gttgtaaatg gcaatgccgt caaacacatc accaccgggc gagtggatat gcagattgat
    10021 ttgttttaaa ttgttgccaa gggcttttaa gtctttagca aattgttggg cggtgacacc
    10081 ccaaaagccg atttcgtcaa aaattgagat ttcggcggtg tcattcgctt tggcttggat
    10141 tgagtaccat ttcatatttt tctccaaaga aaaacgcctg caataaagat tacaggcgtt
    10201 ttaaataact aatttttatt ctttaaatag tatagaataa tccctattgg aacacctatt
    10261 atattgaaca taatagatac aaaattataa gaaaataaat tgtgttctgg gaataactct
    10321 ctaattagta gagaaccaat caaatgaata aaaatcaaaa aaacatagat aaaaatacaa
    10381 taaaaacttg attttcttcc tctatttttc cattttaaac tagataaaaa ggcatatgtt
    10441 agagccaccc aacaactaca tgataatatc caattttcac ttactaatgg cttggtaaga
    10501 taatatgtaa atacatatac ataaaatagt aaggaaaaaa atagccatag taacattatt
    10561 acctcttatt tagattttta tatgctcttt catcaatagg aatagaaacg ttctctctcc
    10621 agttatcttt tatatcataa cttgtaccat ctggattcca ttctccagga atgatattaa
    10681 aaatatcgta tggatcagta aacttatcat aaaagctata atctatacta ccttcaagat
    10741 aaaatctatc tcctttttgt accgcactac ccgtaaaatt gccttcaaca atagcactac
    10801 caattgccca taatgcatta ttaatattcc aaataccttc tttaaaagag taagtatttt
    10861 taaaagaacg agcattttta gtaatgattt gctgaataaa atcaccatgt acgctacggt
    10921 tatctttctt accaagtgca tttggtttct ttactaactc tcggacagta ttaaataatc
    10981 ctaattgttt taaagtaact gatttgccag tcttagctct ataatagtaa agcatatcaa
    11041 aggatgtgac tggagtattt ctatttcgag atgtaatcgg taattttggg gtttgtttta
    11101 tatttgtaac aatatgcctt gataaagttg ttaccagtcg atgactttca tttgaaaact
    11161 cagtaagtgt ttcattgagt atgatcaatg catctattaa tttctgggcc tcattaactt
    11221 catctgcttt atgcttacca acatgtaacc accacaatct tgaaccacct gaaacagata
    11281 aaatcaaatc ataatctctc ggatttacct ttgttaaggc gaatttctta agatttgatt
    11341 tttctgcttt ttctgctatc tcttttttag ataaatatgc tttttttatt ttattaactt
    11401 cgtcatactg tgaaacaagt aacgaataca gtttttttaa cttctcctgc tcaactttat
    11461 ttagaatttt agcatatagc ataagtaacc cactgataat cttaaaaatt tcaccaatag
    11521 atttagccga aataattttc ttttccaact cattgatttt cttacttaac tctacggatg
    11581 agtttgctaa agaaacataa ctttgaactt tatttggggc ataagcaatt ctacgaccaa
    11641 agtaatagtt ttcacgcttc agcttttcca tttcactgaa ttctggtgga aatattggct
    11701 tgattactgg tgggtttgcc acaactttaa ctgtatctaa cacaactgtt gtcataattt
    11761 actccttttc attttcttta gtagaatctt cgagatttct attcgaactg ccgctatttt
    11821 gttggctgta attagttaaa tcggtatcaa atttcagccc ttcggcttgg ttttctcgga
    11881 tttcgacaat ccgttgccgt ttgacttcgg ctgggttatt gccactggct cgaatcgctt
    11941 gcccttcggt tgccagaccg cctttaatcc gctctttcca cgcattcgcc tctttgatgg
    12001 gatcaatcca cggcattaca gggccggaat agacggcatt aaacagcgat ctttcatcaa
    12061 tatcggacgg gattttgata gcttgtgagg caatcgccat tttgagccac tcccgataaa
    12121 tcgggcgact gattgccgcc acaaacgcat cttgcaatac cgcatagcct tcaaaacttt
    12181 caaccaattc ttgtcgctga gcagagtaag tgccgttgta gtctcgggcg atgctcgaat
    12241 agctcgaacg agtaccggcg gcagtggctc gcagttgtcc attgcggaaa gtttccaaat
    12301 tcacatttgg gcggttggaa ttgattaagc caatgtcttc acccggtttt aaatcatcaa
    12361 tcactgcacc gggggcaata tcaaacaggc ggttgccatc gctgttactg tcgtcatcat
    12421 acagtgccgc atcgcctttt ttaatgtaca tcgtcatcgc tgcggcaatg cgtgcggcga
    12481 ctcgttcgct ctcttcgtac tctttcagat ctgccaagcg cacaatcacg ccgtgcaaca
    12541 tactcacgcc acggatttga tgtaaccgct tacgaaaggc gaggtgcaac atattttctg
    12601 ccaacacggt tttcactttg ccgtacattc cgttactttc ttgcggatta tccaaataga
    12661 cttggtaagc ggtaggtttc cgccacgcat tgagaaacac gccttgcacc aagccgtttt
    12721 ttgcctcatc ggattgcatt ggcacaaagt ccggctctaa ggcttcgagt gaaaacgcaa
    12781 tcggcgagcc gtgttctaac cctgccactt tgcctttcac caactgcacg aacacttcac
    12841 cgtctcgtag ccaagttcgc agtagcattc gctctaacag ggggcgagta tataagcctg
    12901 ttacttcggg tttcaccgac cattccgccc acagcttgcg gatttgctct gcgagatctt
    12961 cgtgaacatc accggcaagc gtgagtggct gtggttcgat atgaatacct ttcgagccaa
    13021 tcacccgttc ttccattttg tctaaaatgc cgatcacaat atcgtgattt tggtctaacg
    13081 cccgtgcctg ttcccgtagg ctgaccgcac tttggcgaac agtagagttt gcccctttgc
    13141 tctctcggct tgccttatgg gttcggctag gctgtgccgc ctcataggca tttagcacat
    13201 agcgatttcg agagcgattt gccgcccatt tcggggaaag ggtggcaatg gttttttcga
    13261 ggaagttcat caaataaacc tcgcatattt aattcgatgc tgtttggtgt gttgcccgct
    13321 ttgggcaagt tgttcatcca gcattgtttg atagcgatca cgctgtttgg ttaattccgc
    13381 cacttgatag gataccgacc gcccgttaaa gctcacctgc gattgagcgg tctcaatttt
    13441 ctcatcaagc gtgcggattt tgtctttgag ttcatcgatg gtgtaaaggc tcatagccag
    13501 cctcctgttt ttcgtccgcc accacttagc caactgcttt ttgctttggt cggtttgggt
    13561 tgtggttttg cgggtttttc ttcaatttcg accgcttgtt cagccgttct tggcgtttcc
    13621 cgaatgatgt tagggttgat gtcgggcagt tttgcccagc ttggcacatc gttttcatcg
    13681 ccccatttga ttcgctcgta gcctcgtaaa attgcaatgg cgtgggcata gcagaacagg
    13741 tcgaaggctt cgttattgcc tttgcccggt ttccgccatt tgccgtcggc tcctcgctcc
    13801 tcgtaggtca attcattgaa aaaccattcg cctagccagt ctgggaagtg gatgtagttt
    13861 gcccccaccg tatcacggga aagggcgttg ttaatccgat ctttgaggta gtcggtttgg
    13921 agcagataca acggcacatc gccccgtgcg gaggcgtggc ggtcgctccg tgaggtgttg
    13981 tcggggtgcg ttttggtgat gagcttttgc cgtttggtgc tgtcaccttt gactaaatag
    14041 acccgtttcg catagccatc tcggcggcat tttcgccaaa attggtaggc gttgtcggtt
    14101 acgccctctt cgccgccact gtccaccgcc attgccaaaa tcggcataaa gtcgctcggc
    14161 ttgtgagcaa ggacgtagcg tttttccagc acatctgaaa tcagaatatg ccaatcttcg
    14221 ggaatacgag ggtcgatttt ttcgatcaca ccatctctgt cgggtagtgt gtgcgagatg
    14281 ttgtagcggt caatcaacca gcgttcgcca ttctcgccat agccaaccat ctgcaccaca
    14341 aaacggcgat ttttgccgcc ctgtacatcg actgccgcca cgataaagcg acattgcggt
    14401 ggcacggttt tctcttcaac ctcttcacgg cgttccatta actcatcaga acggcgttgt
    14461 tctaatgcag atcgtggtaa gtaaggtaac ccccagtcgg tatttgttac cgctttgagg
    14521 gtttcctcac tgccagtcat ttcatattcg tgttcggcat tgagcagttt gtaggtgagc
    14581 tgcgcccacg tttggtaggc tgccgctggc ccttcaagcc aaaaggaggc aatacgggat
    14641 ttgcgacttt cgccactaat ttgaccgctt gcatcaattt tttgcccctc tttgagccat
    14701 acgcctttga tgtttagctc tcgtttgagt tcgggcggaa tcaaggcttg gcagtggggg
    14761 cattgtagcc gtgcgttttc actggctttg acaaaatcgc tttcttctcg gtagccaacc
    14821 atatttgcca ttgatggttc aaaatactct gagcaacaag ggcattgcca gtaaaatcga
    14881 cggcgatcac cgcgattgta taggcttaaa atgcctgttg ttggtggagc ctcgtgggtg
    14941 cttttcggga tatgtttgag atcaacaata tctttaccgg gcgaactctc caccaaagtc
    15001 attcctgctg acataaaggt ggtcgtccgc tttgaggcaa gggagaaacc atcaccttca
    15061 ccgtccacat cttcgggcca gcggtcgtag tcagtcagag caacatattt gtagtccgat
    15121 gaagaaagca cattgataga cggccagccg attttgagca agttgcccgc tcggaaatac
    15181 ttgtcgtgga cattgttatc gtttttgcgt gggcttagcc gcttggcaat ttcgggcgaa
    15241 cagcggaatg tgcggtcaag ccgttttcgg ctatgttcgc tggctttctc ttgggtaagt
    15301 tgcaccaaca ggaaatcgga cggatcgcag ataattgagt aggttatcca gccgtcaatc
    15361 aagccgatgg ttttacccgt tcgtgccggc ccgacaaaaa tcaccgcatc atattctcgg
    15421 ctgttgaggc agtccatcgg ttctaacata taggcggcgg tgtgtttatc ccatttgacc
    15481 gagtttcctc cgccaagtgg cacacgcata tattccgcta cggcatcgga gactttcatt
    15541 cggcgaggta ctttgacagc gttcgccata tcacggcgga tttctttggc acttgcaaac
    15601 attagtcatc tccctgttcg gtcgttgggg cttgttggat atggagagcc atctgatctc
    15661 gcacatcatc aattacttgt tgcacccgga taaggtcttt cggctgcaat ccacaatccc
    15721 gctccaaaat atccggcaag gtttcgagtg tttgcactac tgcttttgcc attgcaccca
    15781 tttcaaacgc cacttcggag gctgggatta gctcgcctgt tttctcctca tatttgagac
    15841 gttcgttttc cgcttgccaa aatgacttgc gatcaaccgg agagaggcta tcgacatccg
    15901 ccgacatctt ttccgccaac ccggtgagga ttaaatcacg cagcgcatag agctttaatt
    15961 tgctgttact gccaagcgat ggggtgagcc ctgccactcg ctgagatact gtctggcggt
    16021 gcaatccggt gagttcggcg atctgattga tgttaagttt tagatcaaat aagttatcca
    16081 tttgctcaaa tcctaaaaaa atcaaaaccg cctaaaaaaa cggcaacatc acaggaagat
    16141 gatgatgcct agaaacccaa aaaactgccg aaaaccgcgc tgccgcaacc ccgtggaaag
    16201 gggtatcccc tcgggagtac cttttacaaa ctatgcaaca cattgcttag ttatgatctt
    16261 gatacaatca aaagcataac taagtaatgt aacgcaaatg aaaagggagc aattaagctc
    16321 ccactctcat tagcggttta atccgccaag ttatttaaac ccttgcttag tctgtgcttg
    16381 ccactctcta atacggtcaa tttgactcgc acacaaatca cgctctccca ttactttaat
    16441 gagataatcc accgtatcgc cgtaggtttt accgctaaat gctgtccgct cgcacggcac
    16501 aaggtaagcc gcaggcggat ataaatactc agtgctgacg attgttttgc tagtgcaacc
    16561 gcttaatgct atcagcaaca ccattaggca aatcagcctt agcacaactg tcttgtgcca
    16621 gtattgatgt aatttcattc ttggccacct ccactttatt ccgcagctca tttgcaattt
    16681 tttggctttt ttcgaccgct tgtcgctctt gctctaagct atcggttagc cgttgattgg
    16741 ctttttgctg ctgctcaatg gtttgggctt gtgtttggtt ttcggctctt aagccatcaa
    16801 tctgcttgga ctggtgccaa atccagccac acaaaccca
//
LOCUS       DANKBV010000043        16741 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.043, whole genome shotgun sequence.
ACCESSION   DANKBV010000043 DANKBV010000000
VERSION     DANKBV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16741
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.043"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..437)
                     /gene="lpxK"
                     /locus_tag="PWY43_001151"
     CDS             complement(<1..437)
                     /gene="lpxK"
                     /locus_tag="PWY43_001151"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDL2136037.1"
                     /translation="MKIWQTKSLITWLLSPLSLLFWLISQARKRLYSLNILQSYRSPL
                     PVLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVISRGYGGKAKTFPQLVTRESSAE
                     MVGDEPVLIVQRTNVPLAISPNRQQSIELLLSQFEPDIIITDDG"
     gene            complement(439..843)
                     /locus_tag="PWY43_001152"
     CDS             complement(439..843)
                     /locus_tag="PWY43_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2136038.1"
                     /translation="MKVHEKIKLLRENNHWTQDEMAQKLSMTTKGYAKIERGETISNL
                     PKIEQIAEIFNMDICELLSYGEEGKIYINNTDNNLTNSFISLGNGVEEIQRLQLIISH
                     KDELLAHKEEIIENQKRELALLRDLVETLKNK"
     gene            978..1160
                     /locus_tag="PWY43_001153"
     CDS             978..1160
                     /locus_tag="PWY43_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136039.1"
                     /translation="MNNQYNPLYGMLGILASAASVILPLYAAYKDYQFGENMLSLMDI
                     IIFPLGIVRGLMYLFG"
     gene            1164..1493
                     /locus_tag="PWY43_001154"
     CDS             1164..1493
                     /locus_tag="PWY43_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136040.1"
                     /translation="MKKYLFFAFMTLSNVAYSNLDEWYSASDKERREFIAKHFAEEIV
                     RSDRNTPYDYAMCTGRMFAKQLTRDEVKDYPLANTKPQYLTRDRRIEIMSNLPREILQ
                     NCAKYIQ"
     gene            complement(1529..2281)
                     /locus_tag="PWY43_001155"
     CDS             complement(1529..2281)
                     /locus_tag="PWY43_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL2136041.1"
                     /translation="MAYKPPFTVTTKMLNLVVEISHKLGTLQLDFERNLHLRKNNRLR
                     SIQASLAIEANSLTIEQVTAIIKGKKVLGHPKDIREVKNAYDAYDEMMHYNPYSIKDF
                     LKAHKFLTADLVNDAGAFRSKDVGIYNFSGQLVHMGARPQFVENLINELFSWAKQDDT
                     PALIKSSVMHYEIEMIHPFSDGNGRMGRLWQNVLLAQWNPLFAWLPIETMVYENQQRY
                     YQMLSLADSQNDSTGFIEFMLEMILETLLKLK"
     gene            complement(2320..3567)
                     /gene="clpX"
                     /locus_tag="PWY43_001156"
     CDS             complement(2320..3567)
                     /gene="clpX"
                     /locus_tag="PWY43_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDL2136042.1"
                     /translation="MAFEKEPHCSFCGKKRTEVDQLVEGTEGYICNECIEESYALLHG
                     EEEPLIDENQDDEGSFFTNVPTPHELHAHLDDYVIGQEHAKKVLSVAVYNHYKRLRSA
                     LSNHQTTNGVELGKSNILLIGPTGSGKTLLAETLARRLNVPFAVADATTLTQAGYVGE
                     DVENVIQKLLMKCDFEAEQAERGIVFIDEIDKITRKSESASITRDVSGEGVQQALLKL
                     LEGTVANINPQGGRKHPKGETIPVDTSKILFICGGAFAGLDKIVEARTNTQGGIGFAA
                     ALKKDKDRQDLTELFKQVEPEDLVKFGLIPELIGRLPVVTPLQELDEEALIQILTEPK
                     NAIIKQYQALFEMEGVELHFTKDALISIAQKAISRKTGARGLRSIVENLLLDTMYDLP
                     TLNAKKVTIGKGCVENGDKPKVE"
     gene            complement(3567..4160)
                     /gene="clpP"
                     /locus_tag="PWY43_001157"
     CDS             complement(3567..4160)
                     /gene="clpP"
                     /locus_tag="PWY43_001157"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL2136043.1"
                     /translation="MALVPMVVEQTSKGERSYDIYSRLLKERIIFLNGQVEDNMANLI
                     VAQLLFLEAEDPEKDIYLYINSPGGVVTAGLAIYDTMNFIKPDVATLCTGQAASMGAF
                     LLSAGAKGKRFALPHARIMIHQPLGGARGQATDIQIQAEEILRLKATLTHRMAEHSGQ
                     SYEKVLADTERDNFMSAEEAAEYGLIDKVLTSRSEVA"
     gene            4272..4655
                     /locus_tag="PWY43_001158"
     CDS             4272..4655
                     /locus_tag="PWY43_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HDL2136044.1"
                     /translation="MTLTEIYQKFERCKSWEERYRLLIQLSRLLPKPSEQELAQIAEI
                     EGCESRLWFEFQAEPRKVVAYSDARLMQGILFIVSVALTEKTAEELKGFDLKTMFDEL
                     KISQNLTSTRLNGLGQLQKHIMMNL"
     gene            complement(4759..4835)
                     /locus_tag="PWY43_001159"
     tRNA            complement(4759..4835)
                     /locus_tag="PWY43_001159"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4799..4801),aa:Asp,seq:gtc)
     gene            complement(4924..5679)
                     /locus_tag="PWY43_001160"
     CDS             complement(4924..5679)
                     /locus_tag="PWY43_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2136045.1"
                     /translation="MKKSIYDTPIFFERYQQLRQNPISMNEVVEKPTMFSLLPDLTGK
                     KVLDLGCGTGVHLAHYLTLGAEKVVGLDLSELMLQQAESDLSAHWQKGNDFSLYCLPM
                     ERLNEISEGDFDVVTSSFAFHYIADFSELLAKISQKMTACGTLIFSQEHPIVTCYREG
                     YRWEKNQEKQQVAYRLNYYREEGERDRSWFQQPFKTYHRTMATIVNNLIQGGFEIVQM
                     EEPMLADQPRWHNEFKDLQHRPPLLFIKAIKQK"
     gene            complement(5739..6734)
                     /gene="trpD"
                     /locus_tag="PWY43_001161"
     CDS             complement(5739..6734)
                     /gene="trpD"
                     /locus_tag="PWY43_001161"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDL2136046.1"
                     /translation="MQALLDKLFANQALAQAESHRFFSEVIKGNVSNEQLAAALIALK
                     LRGETVDEIAGAAKAALENANPFSTPDYPFADIVGTGGDGHNTINISTTSAIVAATMG
                     YKIAKHGNRSVSSKTGSSDVLSHLGINVDVSPETARLALDETNICFLFAPTYHSGFKY
                     AAPVRQALKTRTIFNILGPLVNPAHAKRQLLGVYSPELVKVYAETVKALGYEHTIVVH
                     GAGLDEVAVHGETQVAEIQNGQIDYYTLTPEDFGIERHSLEDLKGGEPAENAEKATAI
                     LQGKGKLAHIDAVAANVALLMKTFGEDDLKANVQKVKETLESGKALETLRKLSTY"
     gene            complement(6752..7138)
                     /locus_tag="PWY43_001162"
     CDS             complement(6752..7138)
                     /locus_tag="PWY43_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HDL2136047.1"
                     /translation="MISVYGLKQTLADRRALIADVIFDCMQMSLGVPKQRHALRFDLL
                     DAENFYPPINRSQDFIVIEINLMQGRSEQLKKRLIKGLFSALHTKVGIVPMDVEITIK
                     EQPEHCWGFRGMTGNEATDLEYQVHR"
     gene            complement(7131..7721)
                     /locus_tag="PWY43_001163"
     CDS             complement(7131..7721)
                     /locus_tag="PWY43_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL2136048.1"
                     /translation="MANILFIDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLAKA
                     ETTENCLVALSPGPGNPAEAGNLLEIIRRLKDKVPMIGICLGHQALIEAFGGKVVHTG
                     TVLHGKVSRIEHDNQAMFSGINNPMPVARYHSLMGDELPAEFIVNAKFEHIVMAIRHK
                     TLPICGFQFHPESILTVQGTKLLKQSVEWLLGEKND"
     gene            complement(7731..9287)
                     /gene="trpE"
                     /locus_tag="PWY43_001164"
     CDS             complement(7731..9287)
                     /gene="trpE"
                     /locus_tag="PWY43_001164"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDL2136049.1"
                     /translation="MPQLTITQTELPYFADTTRVYYALCGERPHTLLLDSAEIQSKNS
                     VKSLLFAKSAVHIFCEDQTVTFHALTPNGEAVLPLIANALSLLNPAPTCKFLDKNDRL
                     QAIFPTISNQLDEDSKLKTTTVFDGLRTVSQLFADSTSPIYLGGLFSYDLVTNFIPME
                     NISLQDDGLSCPDYTFYLAEQLVTIDHQTETAVLNTFCFEPNEQTNLNQQAVEFTQKL
                     ANLSHIELPLANQTATSEATANIEDEDFKAIIRKLKHHINIGDVFQIVPSRRFSLPCP
                     NTLASYKQLKINNPSPYMFYMQGENFTLFGASPESALKYSQANRQLEIYPIAGSRPRG
                     FDEQGNIDPELDSRLELELRLDQKELAEHLMLVDLARNDVARVSESGTRRVVDLMQID
                     RYSQIMHLVSRVIGTLRKDLDALHAYQACMNMGTLTGAPKIKAMQLIYQVEQQKRHSY
                     GGAVGYLTSDGNLDTCIVIRSAFVQNGIAYVQAGCGEVLDSDPQLEADETRHKARAVL
                     KAIGQVNAKA"
     gene            9429..10046
                     /locus_tag="PWY43_001165"
     CDS             9429..10046
                     /locus_tag="PWY43_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL2136050.1"
                     /translation="MTQFFYIHPDNPQQRLINQAVEIIRNGGVIVYPTDSGYALGCAM
                     GDKHAMDRIVAIRKLPENHNFTLVCSDLSELSIYALVNNQAYRLIKNNTPNPYTFILP
                     ATKELPRRLMTKRKTVGIRVPDNAIALALISALGEPILSCSLMLPETEMTESDPDEIR
                     DHLQNRVDLIIHGGYLGQQPTTVVDLTDDVPVIIREGSGDPTPFN"
     gene            10192..11259
                     /gene="rluB"
                     /locus_tag="PWY43_001166"
     CDS             10192..11259
                     /gene="rluB"
                     /locus_tag="PWY43_001166"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDL2136051.1"
                     /translation="MTTFQKKSTATFRADKRGESRPSFKKEEQKSERKFGKTSEKPPR
                     PTSKPLTQRIAKKVEKTTASEVASQEKKAKVVGEKLQKILARAGQGSRRELEEIIAAG
                     RVSVDGKMATLGDRVQVSSSTKIRIDGHLVNLMPAQKEICRVLMYYKPEGELCTRSDP
                     EGRATVFDRLPRLSGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVR
                     VFGNIDDAMLQRLRKGVQLEDGPANFKQIKVVGGTGLNQWFDVTLTEGRNREVRRLWE
                     SQGVEVSRLIRIRYGNIKLDKGLPRGGWEEMGLEQVNYLRELVGLPPETETKVEVGVN
                     RRRTNIRQIRKAVKQHQKYRG"
     gene            11324..13096
                     /locus_tag="PWY43_001167"
     CDS             11324..13096
                     /locus_tag="PWY43_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDL2136052.1"
                     /translation="MSWDIVATLAVTGIAVFLFATEKMRMDAVAILVLCSLVLLGQVD
                     TKSALSGFSNSATVTVTAMFVLAAGLQNSGALDSVGSLLAKVKSPWLFLLVLCGVNAA
                     VSPFVNNTAVVAVLIPIVIVAAQNIKMAPSKALIPLSFSSQMAGVCTLIGTSTNLLVN
                     AIAQKQGHSGFGMFEFAPLGIIFLVVGVAYLLVTSRFLLPESHLQLEDGEGFGKYVSE
                     LKVNKDSVLIGKSTFESGLNEEFNLFTIGVLRDGERLSTPSHQVLQKHDILLLRGESE
                     NLAKVREKYGLHHVVYGRRENDEDNLDEDLMVAEVMISPISRWIGGTIPILQQRWNKN
                     ATVLGIQRQSKVIRERLRTTAFKMGDILLLTLPKEDMETLRQDKDFIVLSSDLVKTEE
                     SWKSKFALGVMVLVVATAALGWVPISVSALIGAVAMCVAGCLTAEEAYQSIDWKIILV
                     LAGLLPLGEAMANSGAAQFIVDNTLGKVGEFGPLVVLAVLYLLTMVLTEFMSNAGTAV
                     LLTPIAVSTAKMLGVDASPFIIAVMFAAATSFMTPVGYQTNTMVYGAGGYKFTDFIKI
                     GLPLNVIYWILGIVLIPIFFPFNP"
     gene            complement(13186..>16741)
                     /locus_tag="PWY43_001168"
     CDS             complement(13186..>16741)
                     /locus_tag="PWY43_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL2136053.1"
                     /translation="DGTSGSHNIAIGTYANAKLAGPTTNVTSDDNIAIGNSALANGVN
                     NYKTTAATGETVIGKATAVGSHANATGIVSSAYGAEANATAIHSTAIGAKSQANGDNS
                     TAIGYEAKTSGHGATSLGYYANATANLTTAVGTHAGATSDYASAFGREANASGGSATA
                     LGNRATASGAASVALGVSAKATNQRTIAIGESSNASAFNATAIGRNATAEHTDSITLG
                     SNSVTAIAIPTTNATVNGITYSDFAGTNPIATVSIGAEGKERTITNVAAGRISLSSTD
                     AINGSQLYLTQQAIGNVAATTANILGGGAAVTENGNITFPTYALVNGTPDADKEGKQG
                     RYTTVSAALSALNTAVISPLTFAGDTGTNFERHLGSTVKIKGGSTGILTENNIGVVAD
                     GNSTLTIKLAEKVNLGANGSLTTGDTVVNNTGITIANGVADKPVSLTKSGLDNGGNKI
                     ANVAAGDVDTDAVNVSQLKQAISKFATHYVSISDDGIQRANYDNSGSSGVNSMAIGVA
                     TRANGELATALGSEAEANGERTTAVGPRATADGMNATSIGYNANANATNALAVGSAAN
                     ANADTSTAIGTASTATATRATALGSKSEATGENSTAVGYEASSIGADSLAAGYNANAS
                     GTQSTALGNSANAGGIWSTSVGRNANAAGSSAIALGNSANAAGVASIALGVSSQATTT
                     AAVALGQNAKATHQGSVALGTNSATVATVATKSATLNGNTYTFAGTTPSSTVSIGSVG
                     NERTLTNVAAGRISDSSTDAINGSQLYAAYTEIDGLNTKVNELSNGALTFVDDAGTEI
                     VRKLGTSLNVKGGADATILTDNNIGVVATDANTLTVKLAKDIDLTPAGSVAVGNSKLN
                     NNGLTINNGPSVTMTGVDAGKLKITNVADGDISPISADAVNGSQLYDTANTIATALGG
                     NSSVNANGAVSAPSYTVVDGAPTNEVSKTVNNVGSAITALNDAVTSPLTFAGDTGTPS
                     QRKLGSTVTVKGGVSNESQLTDNNIGVISNGNGSLTVKLAKDIKVNSVTAQTVTANIA
                     VADTVKTGDTTIDTNGLTIVGGPSITKTGINAAGTKVTNVKAGTEDTDAVNFSQLKAT
                     EKNINNKINNIDSKVNKVDKRLRAGIAGATATAGLPQAYLPGKSMLATAGDTYRNEAA
                     IAVGYSRISDNGKVIYKLTGNSNTRGDFGGSISMGYQW"
BASE COUNT         4619 a         3377 c         3556 g         5189 t
ORIGIN      
        1 ccatcatcgg tgatgatgat gtccggctca aactgactta atagtaattc aatgctttgt
       61 tggcggtttg gagaaatagc caaaggcaca ttggttcgct gaacaatcag aaccggttca
      121 tcacccacca tttcagcaga actttctcgg gtaactaatt gcggaaacgt tttcgcttta
      181 ccgccatagc ctcgggaaat cacacctact ttcacgccac gctgttgcaa ttgctctacc
      241 agccaaacca ccactggcgt tttgccgttg cctccaaccg agatattacc gactaccagc
      301 acaggcaaag gcgaacggta cgattgcaaa atattcagag aatataaccg cttgcgagct
      361 tgactaatca gccaaaacag caacgaaagc ggcgaaagca gccaagtgat aagggatttt
      421 gtttgccaga ttttcatatt atttattttt aagtgtttca actaaatctc gtaataaggc
      481 taattcacgt ttttggtttt cgataatttc ttctttatgg gctaataatt catctttgtg
      541 ggagataata agttgtaaac gttgaatttc ttctactcca tttcctaatg agataaatga
      601 attagtgaga ttattatctg tattatttat gtaaattttt ccttcttccc cataggataa
      661 taattcgcaa atatccatat taaatatttc tgcgatttgt tctattttcg gtaaattaga
      721 gatagtttca ccacgttcta tttttgcata acctttggtt gtcatactta atttttgagc
      781 catttcatct tgtgtccaat gattattttc tcgcagtaat ttgatttttt cgtgtacttt
      841 catataacac ctatagattg tgggagttgg gatataactt gatgtggtaa aggataaatt
      901 ttgtggattg tagcagttta ttgagcaatt tagaatagag taaattttat cttaagctca
      961 caggagaaga aacattcatg aataatcaat ataatccatt atatggaatg ctaggtattt
     1021 tagcgtcagc tgcatcagta attttgcctc tttatgcagc atataaagac tatcaatttg
     1081 gggagaatat gttatctctt atggatataa ttattttccc gttaggtatt gttcgtggac
     1141 ttatgtattt atttggatag attatgaaaa aatatttatt ttttgctttt atgacattaa
     1201 gtaatgttgc ttattctaat ttagatgagt ggtactcagc ttcagataaa gagcgaagag
     1261 aatttatcgc aaaacatttt gcagaagaaa tagtaagaag tgatagaaat acaccgtatg
     1321 attatgctat gtgcactggt agaatgtttg ctaaacaact gactcgagat gaagtcaaag
     1381 actacccttt ggcaaacact aagccgcaat atttaacccg tgatagacgg attgagatta
     1441 tgtctaattt acctcgtgaa atattacaaa attgtgctaa atacatacag taattttatg
     1501 agtaacacca ctttggaatt aagtggtgtt attttaattt taataatgtc tctaaaatca
     1561 tctccaacat aaactcaata aagccggtag aatcattttg gctgtctgcg agagaaagca
     1621 tttgataata acgttgttga ttttcgtaaa ccattgtttc aatcggcaac caagcgaaaa
     1681 gtgggttcca ttgtgctaaa aggacatttt gccataatcg acccattctg ccgtttccat
     1741 cactaaaggg gtgaatcatt tctatttcat agtgcataac cgagctttta atgagtgcag
     1801 gtgtatcatc ttgtttagcc caagagaaca actcattaat caaattctct acgaattgag
     1861 gacgagcccc catatgcaca agttgcccac tgaaattata aatgccgaca tcttttgaac
     1921 gaaaagcccc tgcgtcattt actaagtcag cagtaagaaa cttatgtgct ttgagaaaat
     1981 ctttaatcga gtaaggatta tagtgcatca tttcatcgta agcgtcatag gcatttttga
     2041 cttcacgaat atctttgggg tgtccgagaa cctttttgcc cttaatgatt gcggtaactt
     2101 gctcaatcgt taatgaattt gcctcaatag ctaaagaggc ttgaattgat ctcaagcggt
     2161 tattttttct aagatgtaaa ttacgctcaa aatccaattg tagcgttccc aatttatgag
     2221 aaatttctac aaccagattc agcattttag tggtaactgt aaaaggtggt ttataagcca
     2281 ttttgcaata atcctaaaaa attaaaccgc ttgtcattgt tactccacct tcggcttatc
     2341 gccgttttca acacagcctt tgccgatggt gacttttttc gcattgaggg ttgggagatc
     2401 gtacattgtg tcaagtaata agttctcgac aatagaacgt aacccacgag ccccggtttt
     2461 gcggctaatc gctttttggg caattgaaat taatgcatct ttggtgaagt gcaattcaac
     2521 gccttccatt tcgaataagg cttgatattg cttgatgatc gcatttttcg gctcggttaa
     2581 gatttgaatc agagcttctt cgtccaattc ttgcagcgga gtcaccaccg gtaaacgccc
     2641 gattaattca ggaattaagc cgaatttgac taaatcttcc ggttcaactt gtttgaatag
     2701 ctcggttaaa tcttggcgat ctttatcttt tttcaatgcc gctgcaaaac cgataccacc
     2761 ttgagtgttg gtacgggctt ccacgatttt atctaaaccg gcaaaagcac cgccgcaaat
     2821 aaagagaatt ttggaggtgt ccaccggaat ggtttcgcct ttcgggtgtt tgcgaccgcc
     2881 ttgtgggtta atgttcgcca ccgtgccttc aagtagtttt aataaggctt gttgaacacc
     2941 ttcgcccgat acatcacggg taatggatgc actttccgat ttacgggtga ttttgtcgat
     3001 ttcatcaata aaaacaatgc ctcgttccgc ttgttcggct tcaaaatcgc atttcattaa
     3061 taatttttga atcacgtttt cgacatcttc tcccacatag cctgcttggg taagggtagt
     3121 agcatcagct accgcaaaag gtacgtttaa acggcgggca agggtttctg ccaacaaggt
     3181 tttaccgcta ccggtcggcc cgattaataa aatattactt ttacctaact ctacgccatt
     3241 agtggtttga tggtttgaca atgcacttct taagcgtttg tagtggttat aaacggccac
     3301 cgaaagcact tttttcgcgt gttcttgacc gatgacataa tcatctaaat gagcgtgtaa
     3361 ttcgtgaggt gtcggcacat tggtgaaaaa actgccttca tcgtcttggt tttcatcaat
     3421 taacggctct tcttcaccgt gtaataaggc gtaggattcc tcgatacatt cattacaaat
     3481 atagccttct gtgccttcga ctaattgatc gacttcggta cgttttttac cgcaaaaact
     3541 acagtggggt tctttttcaa atgccattac gccacctcac tgcgagaagt taatactttg
     3601 tcgattaagc catactcagc ggcttcttcc gcagacataa aattatcacg ctcggtatcg
     3661 gctaacactt tttcatagct ctgaccgctg tgttctgcca tacggtgggt aagggtagct
     3721 ttcaagcgta aaatttcttc cgcttgaatt tgaatatcgg ttgcttgacc tctcgctcca
     3781 cctaaaggtt ggtgaatcat aatacgagcg tgcggcagag caaagcgttt accttttgcc
     3841 ccggctgaaa gcaaaaatgc ccccattgaa gccgcttgcc ctgtgcaaag ggttgctaca
     3901 tcgggtttga tgaagttcat tgtatcgtaa attgccaagc ctgctgttac cacgccaccc
     3961 ggtgagttaa tgtataaata gatgtctttt tccggatctt cggcttctaa aaacagaagt
     4021 tgtgccacaa tcaggtttgc catattgtct tccacctgac cgtttaaaaa aataatacgt
     4081 tcttttaata aacgtgaata aatgtcgtaa gaacgttcgc cttttgaggt ctgttccacc
     4141 accattggga ctaatgccat tttgttttcc tctgtcgtaa tacaaaatct cgctattcta
     4201 gcaaattttc agaaaatcat taacaacaag cggtcagatt gttaaaatct tttgcaaatg
     4261 atgaggaaat tatgacatta acagaaattt accaaaaatt tgaacgttgt aaatcgtggg
     4321 aagaaagata ccgcttgtta attcagctaa gtcgcttgtt accaaaacca agtgagcaag
     4381 agttggcaca aatagcggaa attgagggct gtgaaagccg tttatggttt gaatttcaag
     4441 ctgagccacg aaaagtagtg gcatatagcg atgcccgttt aatgcagggg attttattta
     4501 ttgtgagtgt agctttaact gaaaaaacgg cagaagagct gaaaggcttt gacttaaaaa
     4561 cgatgtttga tgagctaaaa atttcacaaa atttaaccag cacaaggtta aatgggttgg
     4621 gtcagttaca gaaacatata atgatgaatt tgtaatttac cctctcccac aaggggcgag
     4681 ggtaaataaa atccgcagga ttttattcgg gagaggggaa tataaaaaac ctctgatttc
     4741 tcagaggctt aaatttgttg gcggaacgga cgggactcga acccgcgacc ccctgcgtga
     4801 caggcaggta ttctaaccag ctgaactacc gctccgtact ggtgctaaaa taatagtggt
     4861 ttcctcaaca ttgagcaaat agtttttgca aaatagtgtt taattggttg taaaataacc
     4921 actttatttc tgcttgatcg ccttgataaa cagcaacggc ggacggtgct gtaaatcttt
     4981 aaattcattg tgccaccgag gttggtctgc cagcattggt tcttccattt gcacaatctc
     5041 aaacccgcct tgaataaggt tattcacaat ggttgccatt gtgcggtggt aggttttaaa
     5101 gggttgttga aaccaacttc tgtcccgttc gccctcttcc cgataatagt tcaagcggta
     5161 agcaacttgt tgtttttctt ggtttttctc ccaacgatag ccttctcggt aacaggtgac
     5221 aatcgggtgt tcttgcgaga aaatcagcgt gccacaagca gtcatttttt gcgaaatttt
     5281 tgcaagtagt tcggaaaaat cagcaatata atgaaacgca aaagagctgg ttactacatc
     5341 gaaatcgccc tcgctgatct catttaatcg ctccatcggc aggcagtaaa gggaaaaatc
     5401 gttgcctttt tgccaatgag cagaaagatc gctttctgcc tgttgtagca ttaattcaga
     5461 taaatccaac cccaccactt tttctgcacc gagcgttaaa taatgggcta aatgaactcc
     5521 tgtgccacaa cctaaatcca gcactttttt gccggttaag tctggcagta gagagaacat
     5581 tgttggcttt tccaccactt cattcatact tattggattt tgtcgaagtt gttggtaacg
     5641 ctcaaagaaa atcggagtgt cgtaaatgct ttttttcact taatagcctt taaacaaata
     5701 taaaataagg cgggttaaaa cccgccacta cgatatgatt agtaagtcga taattttcgc
     5761 aaagtttcta acgcttttcc actctcaagc gtttctttta ctttttgcac attggctttt
     5821 aaatcatctt ctccaaaggt tttcattaat aatgcgacat ttgctgccac cgcatcaata
     5881 tgagccagtt taccttttcc ttgtaaaata gcggttgctt tttcggcatt ttctgcaggt
     5941 tcaccgcctt tgagatcttc taacgaatgg cgttcaatgc cgaaatcttc cggcgttaag
     6001 gtgtaatagt cgatctgtcc gttttgaatt tccgcaacct gcgtttcgcc gtgaacggca
     6061 acttcatcta aacctgcacc gtgtaccaca atagtgtgtt cgtaacctaa ggcttttacg
     6121 gtttcagcat agactttcac taactcaggg gagtacacac cgagtaattg gcgtttagcg
     6181 tgagccgggt ttaccaacgg ccctaaaata ttgaaaatcg tgcgggtttt taaggcttgg
     6241 cgaacaggag cggcatattt gaatccgctg tggtaggttg gggcaaacaa aaagcagatg
     6301 ttggtttcat ctaaagcaag gcgtgccgtt tcaggtgaaa cgtcaacatt aatacctaaa
     6361 tggcttaata catcgcttga gccggtttta cttgatacgc tacggttgcc gtgtttggca
     6421 attttatagc ccattgtggc ggcaacaatc gcacttgtgg tagaaatgtt gatggtatta
     6481 tgcccatcgc cacctgtgcc gacaatatcc gcaaaagggt aatcaggcgt tgagaacgga
     6541 ttggcatttt caagtgcggc ttttgcagca cctgcaattt catcaacggt ttcgccacgc
     6601 agttttagag cgattaaggc agcagcaagt tgttcatttg atacattgcc tttaataact
     6661 tcactaaaaa aacggtgaga ttccgcttgg gctaaggctt ggttggcgaa taatttatct
     6721 aaaagtgctt gcatagtctc ttccttttta attagcgatg tacctgatat tccaaatcgg
     6781 ttgcctcgtt gccggtcatt ccacgaaagc cccagcaatg ctctggctgt tctttaatgg
     6841 tgatttcaac gtccatcggc acaatgccga ctttggtgtg taaggcacta aatagtcctt
     6901 taattaagcg ttttttgagc tgttcgctcc gcccttgcat caggtttatt tcaatcacaa
     6961 taaaatcttg gctacggtta attgggggat agaaattttc cgcatccaat aagtcaaaac
     7021 gcaaagcgtg gcgttgtttt ggtacgccaa ggctcatttg catacagtca aaaatcacgt
     7081 ctgcaatcag ggctctgcgg tctgctaagg tttgttttaa tccatataca ctaatcattt
     7141 ttttctccta ataaccattc caccgactgc ttaagtaatt ttgtgccttg cacggttaaa
     7201 atggattccg ggtggaactg gaaaccgcaa atcggtaagg ttttgtggcg aattgccatc
     7261 acaatatgtt caaatttcgc attaacgata aattctgccg gtaattcatc gcccattaat
     7321 gagtgataac gagcgacagg cattggattg ttaatgcctg aaaacatcgc ttggttatcg
     7381 tgttcaatac gagaaacttt gccgtgcaat actgtgccgg tatgcactac tttgccgcca
     7441 aaggcctcaa ttagagcttg atgtcctaaa caaatgccaa tcatcggtac tttgtctttc
     7501 agacggcgaa taatttccaa taaattgcct gcctcagccg gattacccgg gccgggggaa
     7561 agggcaacca aacaattttc ggtggtttca gctttggcta aaaaatcctc aagcggataa
     7621 tcattgcgga aaatcgttac ctgatgccct aattcacgaa attgatcgac caagttatag
     7681 gtaaaagagt cgaaattatc aataaaaaga atatttgcca tagtttgtcc ttatgctttt
     7741 gcattcacct gcccaatcgc ttttaacacc gcgcgtgctt tatggcgtgt ttcatctgcc
     7801 tctaattgtg gatcggaatc cagcacctca ccacaaccag cttgcacata ggcaatgcca
     7861 ttttgaacaa aggcggaacg gatcacgata caggtatcta aattgccgtc tgaggttaaa
     7921 tagcccaccg caccgccgta gctatggcgt ttttgttgct cgacttggta gatcagttgc
     7981 attgccttaa ttttcggagc accagttagc gttcccatat tcatacaagc ttgataagcg
     8041 tgcagagcgt caagatcttt tctaagtgtg ccgatcacac gagaaactaa gtgcataatt
     8101 tgcgaatagc ggtcaatttg cattaaatca accacacggc gagtgccgga ttcgctcaca
     8161 cgagccacat cattgcgggc aagatcgact aacattaaat gttcggcaag ctctttttgg
     8221 tctaaacgca gttctaattc caaacgggaa tctaattcag gatcgatatt accttgttca
     8281 tcaaaaccgc gtgggcgaga gccggcaatc gggtaaattt ccaactggcg gttggcttgc
     8341 gaatatttca acgcactttc aggcgacgcc ccaaacagag tgaaattttc gccctgcata
     8401 tagaacatat acgggcttgg attgttgatt ttgagctgtt tatagctggc aagagtattc
     8461 gggcaaggca agctgaaacg gcgagagggc acaatttgga acacatcacc gatattaata
     8521 tggtgtttga gtttgcggat aatcgctttg aaatcttcat cttcaatatt ggcggttgcc
     8581 tcactcgttg cggtttggtt cgctagaggc agttcaatat gtgacagatt tgcaagtttt
     8641 tgcgtaaatt cgaccgcttg ttggttcaaa ttggtttgtt cattcggttc aaaacagaag
     8701 gtgtttagca ccgcagtttc ggtttggtgg tcgattgtaa ccagttgctc tgctaaatag
     8761 aaagtgtaat ctgggcaact taagccatca tcttgcaggc tgatattttc catcggaata
     8821 aaatttgtta ccaaatcgta gctaaataat ccgcccaaat agataggcga tgtggaatca
     8881 gcaaacagtt ggctcaccgt tcttagcccg tcaaacacgg tggtggtttt gagcttgctg
     8941 tcttcatcta actggttgct gatggttggg aaaatcgctt gcaagcggtc gtttttgtct
     9001 aaaaatttgc aagtcggagc agggtttaac aggctcaatg cgtttgcaat caaaggtaaa
     9061 accgcttctc cgtttggggt taaagcgtgg aaagtcacgg tttgatcttc acagaaaata
     9121 tgcactgccg atttcgcaaa cagcaaactt tttacactgt ttttactttg aatttccgct
     9181 gaatctaata atagcgtatg tgggcgttcg ccgcataatg cgtaataaac acgggtcgta
     9241 tcggcaaaat agggtaattc tgtttgagta atcgttaatt gaggcatagc aaattcctat
     9301 tcaatttttc gtactattaa actagtacat taaaaagaag tcaagcaatt attccagata
     9361 attatgcgag cttgggcgaa cttggtgtat aattagccca atttttagaa agtcgaaggc
     9421 ttgagactat gacacaattt ttttatatcc accccgacaa cccacagcaa cgcttaatta
     9481 accaagcggt agagattatt cgaaacggtg gcgtaattgt ttaccctacc gattcaggct
     9541 atgctctcgg ttgtgcaatg ggcgataagc acgcaatgga tagaattgta gcaattcgta
     9601 aattgccgga aaatcacaat tttaccttag tttgtagtga tttatccgag ctttctatct
     9661 acgcgttggt caataatcaa gcctatcgct taattaagaa taataccccg aatccttata
     9721 cttttattct gccggcaact aaggaacttc ctcgcaggct gatgacaaag cgtaaaacag
     9781 tggggattcg tgtgccggat aatgcgatcg cattggcgtt aatttccgcc ttaggtgagc
     9841 cgattctttc ttgctcgtta atgttgccgg agacggaaat gaccgagtca gaccctgatg
     9901 aaattcggga tcatttgcaa aatcgggtgg atttaattat ccacggtggt tatttagggc
     9961 agcagcctac aacggtagtt gacttaaccg atgacgtgcc ggttattatt cgagaaggat
    10021 cgggcgatcc aacaccgttt aattaaattg atgttgtatg attaatatat agaattatgc
    10081 gtaggggggc tgtgccagtc cgtattaata tcgttatttg gacagacaca ggacctgtcc
    10141 ctacaatgaa aatcaaattc agacgcttgt gaaagcgaca aaaggaacct tatgacaact
    10201 tttcaaaaaa aatcaactgc gactttccgt gcagacaaac gtggtgaaag ccgtccctca
    10261 tttaaaaaag aagagcaaaa atctgaacgc aaatttggta aaacgtcaga aaaaccacca
    10321 cgtccaactt ctaagccatt aacgcaaaga attgcaaaaa aagtggaaaa aacgaccgct
    10381 agtgaagtgg catctcaaga gaaaaaagcc aaagtagtag gtgaaaagct acaaaaaatc
    10441 ttagcgcgag cagggcaagg ttctcgccgt gaattagaag agattatcgc tgctggacga
    10501 gtgagcgtgg acggtaaaat ggctactctt ggtgatcgtg tgcaggttag ctcatcgact
    10561 aaaatccgca ttgacggaca tttagttaat ttaatgccgg cacaaaaaga gatctgccgt
    10621 gtattaatgt attacaaacc ggaaggggaa ctttgcaccc gttccgatcc ggaaggacga
    10681 gccaccgtat ttgaccgctt gcctcgttta agtggcgctc gttggattgc ggtcgggcgg
    10741 ttagatatta acacctcagg cttattattg ttcactaccg atggcgaatt agccaaccgt
    10801 ttaatgcacc caagccgtga agtcgaacgt gaatattcgg tgcgtgtgtt cggcaacatt
    10861 gacgatgcga tgttacaacg cttacgcaaa ggcgttcagt tggaagacgg accggcaaac
    10921 tttaaacaaa ttaaggttgt gggtggcaca ggcttgaacc aatggtttga tgtgacttta
    10981 accgaagggc gtaaccgtga ggttcgccgc ctgtgggaat cgcaaggggt agaagtcagc
    11041 cgtttgattc gtatccgtta cggcaatatc aaattagata aaggcttacc tcgtggcggc
    11101 tgggaagaaa tgggcttaga acaagtcaat tacttgcgtg agctggtagg gttaccgccg
    11161 gagactgaaa ctaaagtgga agtgggggta aaccgccgcc gaaccaatat ccgtcaaatt
    11221 cgtaaagcgg taaaacaaca tcaaaaatat cgtggctaaa caccatcttt ggggctgcat
    11281 tagttctgct aatgcagcta ttttctttca aaaaggagca tttatgtctt gggatattgt
    11341 tgcaacattg gcggtcactg ggattgccgt atttttattt gcaactgaaa aaatgcgaat
    11401 ggacgcagtt gcgattttag tgctgtgttc attagtatta ttagggcagg ttgataccaa
    11461 atctgctctg agtggatttt caaactcagc tactgtaacc gtaactgcaa tgtttgtatt
    11521 agctgctggt ttacaaaata gtggtgcttt agatagtgta ggcagtttac ttgctaaggt
    11581 aaaatcccct tggttattct tgcttgtact ctgtggcgta aatgctgctg tttctccttt
    11641 tgtgaataat acggcagtgg tcgcagtatt aattccgatt gtgattgtcg cagcacagaa
    11701 tataaaaatg gctccttcca aagcccttat tccgttatca ttttcttctc aaatggcagg
    11761 tgtttgtaca ctgatcggca cttccactaa tctattagtt aatgcaatcg ctcaaaagca
    11821 agggcattcc ggtttcggta tgtttgaatt tgcccctttg gggattattt ttttagtggt
    11881 tggggttgcc tatttattag tgaccagccg tttcttatta cccgaatcac atttgcaact
    11941 tgaagatggt gaggggtttg gaaaatatgt ttctgaactt aaagtaaata aagactctgt
    12001 attaattggc aaaagtacat ttgaatcagg attaaatgaa gaatttaatc tatttactat
    12061 tggtgttttg cgtgatgggg aaagattatc gacaccgagt catcaagtat tgcaaaaaca
    12121 tgatatttta ttgttgcggg gtgaatcgga aaatttagca aaagtacgag aaaaatatgg
    12181 attgcaccac gttgtgtatg gacgccgtga gaatgatgaa gataatctgg atgaagactt
    12241 gatggtggct gaagtgatga tttctcctat ttctcgttgg ataggaggga ctattcctat
    12301 tttacaacag cgttggaata aaaatgcaac ggtgttaggc attcaacgcc aaagtaaagt
    12361 aattcgtgag cgtttacgca ccactgcttt taaaatgggg gatattttat tactcacatt
    12421 accaaaagag gatatggaga cgttacgcca agataaagac tttattgtgt tatcttctga
    12481 tttagtcaaa acggaggaat cgtggaaaag taaatttgct ttaggggtaa tggttttggt
    12541 ggtggcaaca gcagccttag gctgggttcc tatttcagtg agtgccttaa ttggtgcagt
    12601 tgcgatgtgt gttgctggtt gtttaacggc tgaagaagcc tatcaatcta ttgactggaa
    12661 aatcatttta gtcttagctg gattactgcc attaggtgaa gcgatggcaa atagtggggc
    12721 ggcgcaattt attgttgata acacgcttgg taaagtaggg gagttcgggc ctttagtggt
    12781 gttagctgta ttatatttac tgacaatggt gttaaccgaa tttatgagta atgcagggac
    12841 tgccgtgttg cttacgccta ttgcggtttc taccgctaaa atgttagggg tagatgcttc
    12901 cccattcatt attgccgtaa tgtttgcggc tgcgaccagc tttatgaccc ctgttggcta
    12961 tcaaaccaac acgatggtat acggtgcagg tggctataaa ttcacagatt ttattaaaat
    13021 tggtttaccg ctaaatgtaa tctattggat tttagggata gtattaattc cgattttctt
    13081 cccttttaat ccataattga tagataatta tagaaaaaag gtttacagat cagaaactct
    13141 gtaaaccttt tttattcagc aataaatcaa ttttctattg gaaaattacc attgataacc
    13201 catgctaata ctaccaccaa agtcgcctct ggtattgctg ttcccagtta atttatagat
    13261 aaccttcccg ttatctgaaa tccgagaata acctactgcg attgcagctt cattacggta
    13321 tgtatctcca gccgttgcta acatactttt acctggtaga tatgcttgcg gtaaacctgc
    13381 ggttgcagtt gcaccagcaa taccagcgcg taaacgttta tcaactttgt ttacttttga
    13441 atcaatatta ttgatcttat tattgatatt tttttctgtt gcttttaatt ggctaaagtt
    13501 aactgcatca gtatcttcag ttcctgcttt tacgttagtc acttttgttc ctgctgcatt
    13561 aatacctgtt ttagttatac tcggtccccc tacaatagtt aatccattag tatcaatggt
    13621 tgtatcacca gtcttaacag tatcggctac agcaatgtta gctgttactg tttgagctgt
    13681 gacagaatta actttaatat ctttagctaa tttaaccgtt aatgaaccat taccatttga
    13741 aataaccccg atattattat cagttagttg tgattcgtta gataccccac cttttactgt
    13801 aacagtagaa cctaatttac gttgagatgg cgtgccggta tcacctgcaa atgttagtgg
    13861 gctggttact gcatcattta acgcagtaat tgcagagcct acattattga ccgttttact
    13921 aacttcattt gtaggagcgc catcaaccac agtatagctt ggggctgaaa cagcaccatt
    13981 tgcatttaca cttgaattac cacctaaagc ggtagcaata gtatttgccg tatcgtaaag
    14041 ttggctacca ttaactgcat ctgctgagat aggtgaaata tcaccatcag ctacattagt
    14101 tatttttaat ttgccagcat cgacacctgt cattgtgaca cttgggccat tattaatggt
    14161 taagccgttg ttatttagtt ttgaattacc aacagctacg gagccggcag gagttaaatc
    14221 aatgtctttc gctaatttaa ctgttaatgt gttagcatct gttgccacta caccgatatt
    14281 attatcagtc aatatggttg catcggcccc acctttaaca ttcagagaag tacctaattt
    14341 acgaacaatt tcagtaccag catcatctac aaaagtaagt gctccattac ttaactcatt
    14401 cactttcgtg tttaaaccat caatttcggt ataagcagca taaagttgtg aaccgttgat
    14461 agcatccgtt gatgagtccg aaatacgacc tgctgcaaca ttcgttaaag tacgttcatt
    14521 gcctacagaa ccaatactta cagttgagct tggcgtagta cctgcaaatg tgtatgtatt
    14581 gccatttagc gttgcacttt ttgttgcaac tgtggctact gttgcagagt tagtacctaa
    14641 tgcaacagaa ccttggtgtg ttgcttttgc attttggcca agagccactg cagcagttgt
    14701 tgttgcttgt gatgaaacac ctaatgcaat agaagctaca ccggctgcat ttgcactgtt
    14761 acctaaagca atcgcagagc tacctgcagc attggcattt ctacccacag aagttgacca
    14821 tattccacct gcattagcag agttacctaa agcggttgac tgtgtacccg aagcatttgc
    14881 gttatagcct gcagcaagag aatctgcacc gattgaactt gcttcataac ctacagcggt
    14941 tgaattttca ccggtagctt cagatttaga gcctaatgca gttgcacggg tagctgtcgc
    15001 tgttgaagct gtaccaatag ctgttgaggt atcagcattt gcattggctg cagaaccaac
    15061 cgcgagggca tttgttgcat ttgcattcgc attgtagcct atagaagttg cattcatacc
    15121 atcagctgtt gcgcgaggac caactgcagt tgtacgttca ccgtttgctt ctgcttcaga
    15181 gcctaatgcg gttgccagct caccattagc tcttgttgca acgccgatag ccattgaatt
    15241 aacaccgcta gagcctgaat tatcatagtt agcacgttga atgccatcat ctgaaatact
    15301 aacataatga gttgcaaatt tgctgatagc ttgtttgagt tgactgacat tgaccgcatc
    15361 agtatctaca tcgcctgcgg caacattggc gattttatta ccgccattat ctaagccaga
    15421 tttagtaaga cttaccggtt tgtctgcaac gccgttagcg atggtgatac ctgtattgtt
    15481 aacaacggta tcacctgttg ttaatgaacc attagcgcct aagttaactt tttctgctaa
    15541 ttttatggtt aaagtactat tgccatcagc tacaacacca atattattct cggttaatat
    15601 gcctgtagat ccgcctttga tcttgactgt tgaacctaga tggcgctcaa aattagttcc
    15661 tgtatcaccg gcaaaggtta acggactgat caccgctgtg tttaatgcgc ttagtgctgc
    15721 agaaacagta gtgtatctcc cttgtttgcc ttctttatct gcatctggtg tgccattcac
    15781 taaagcgtag gttgggaaag tgatatttcc attttcagta acagctgccc caccacctaa
    15841 gatatttgcg gttgttgcag caacattacc tatagcttgt tgtgttaaat ataattgtga
    15901 accgtttatg gcatctgtag agcttaaaga tatacgtcct gcagcaacat tggtgatagt
    15961 tctctctttg ccttccgccc caatacttac tgtagcaatt ggatttgttc cagcaaagtc
    16021 actatatgta ataccattta cagttgcatt tgtagttggt atggctatag ctgtaactga
    16081 gttgctacct agcgttatcg aatcggtatg ttcagcagtg gcatttcgac ctatagcggt
    16141 tgcattaaag gcggaagcat ttgatgattc tccgattgca attgtgcgtt gatttgttgc
    16201 tttagcgctt acccctagtg caacagaagc cgcgcctgat gcagtagcac gattgcctaa
    16261 agcggttgca gaacctccac ttgcattggc ttcacgacca aatgcagaag cataatcgct
    16321 ggttgcaccc gcatgagtac caacagcagt agttaaattt gctgttgcat ttgcataata
    16381 acctaatgag gttgcaccgt ggcctgaagt cttcgcttca taaccaattg ctgttgagtt
    16441 atcgccattt gcttgagatt ttgcaccaat tgctgtggag tgaatagctg ttgcgttagc
    16501 ttcagcacca taggcggagc taacaatacc agttgcattg gcatgactac ctacagcagt
    16561 tgcttttcct attaccgttt ctcctgttgc tgctgtagtt ttgtaattat taacaccatt
    16621 tgcaagagcg gaattaccga tggcgatatt atcatcactg gttacatttg tagtaggtcc
    16681 tgctagtttt gcattagcat aagtaccaat tgcaatattg tggctaccac tagtaccgtc
    16741 t
//
LOCUS       DANKBV010000044        16735 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.044, whole genome shotgun sequence.
ACCESSION   DANKBV010000044 DANKBV010000000
VERSION     DANKBV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16735
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.044"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..615)
                     /locus_tag="PWY43_001169"
     CDS             complement(<1..615)
                     /locus_tag="PWY43_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2136054.1"
                     /translation="MAEILFRSCLCAMQSVFFIYLQFYKGFETSQIATAFSLLVFTSQ
                     AFSLFAGSWGDSFGRYPIMMLGCLLDALAYILLLTTKNYPLLLLATFFFGLGSTLFST
                     NAKAFLLANIQDTGDSYQLKTKAQGKFLKVSSLSAMVAPLLTIPFIFYRKAEWLIWIS
                     CCIEIIMLFVMFKTAPKTHECANGQCERFKFGALKGILNEQFIFV"
     gene            698..1159
                     /locus_tag="PWY43_001170"
     CDS             698..1159
                     /locus_tag="PWY43_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL2136055.1"
                     /translation="MRKIKLLHVILSLILGLSAAAIGSYPAIKEIGEERAKKHQDILD
                     LNRELYSAMVNRDFSALQTLLSDDFELYSSTGEVIDKQEWIKLIQKRVLEFDKVDLLS
                     TDFQGNQSSGLLRVNGEFFGIERDNVVVEMNIRTIVRNTERQIKCIVIKEV"
     gene            1172..2065
                     /gene="murQ"
                     /locus_tag="PWY43_001171"
     CDS             1172..2065
                     /gene="murQ"
                     /locus_tag="PWY43_001171"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2136056.1"
                     /translation="MNALGQMITEQRNPNSMNIDRLSALDIVQVINQEDKQVAIAVEQ
                     CLPQIAQAVEKIVQAFEKGGRLVYVGAGTSGRLGVLDASECPPTYGVKPEMVVGLIAG
                     GERALRFPIEGAEDNREQGKVDLQAVNFSEKDVLVGIAASGRTPYVLGAMEYAKQLGA
                     TTVSIASNPNSAMAQIADIAIETVVGPEVLTGSSRMKSGTAQKLVLNMLTTASMILIG
                     KCYQNLMVDVQATNEKLKARAIRIVMQATECEREVAEDTLKLAENNAKLAIMMILGNL
                     DKQKAEDLLSQNQGRLQKALK"
     gene            complement(2151..3095)
                     /gene="prmB"
                     /locus_tag="PWY43_001172"
     CDS             complement(2151..3095)
                     /gene="prmB"
                     /locus_tag="PWY43_001172"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2136057.1"
                     /translation="MFKYNTALLDEISALPIVNDLVTIQDMMRWGYSYFNASDLYYGH
                     GYDNAWDEAQQLVLAGLYLPFDVPETLYSSRLTRVEKERIINLICERLGSRKPVAYLT
                     NSAWFCGGEYYVDERVIVPRSPIGELIKKGFKGIIKNEPHRILDMCTGSGCIAIACAE
                     RFPEAEVDAVDLSLDALDVAQINIERHQVSHRVFPICSDLFNNLPQDKYDLIVTNPPY
                     VDEEDLGDMPEEFHHEPALALGSGKDGLDLTKRILAQAADYLNDDGVLVCEVGNSMVH
                     LIEQFPSVPFNWLEFEHGGLGVFSLTKEELVKHRHLFA"
     gene            3188..3733
                     /gene="smrB"
                     /locus_tag="PWY43_001173"
     CDS             3188..3733
                     /gene="smrB"
                     /locus_tag="PWY43_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDL2136058.1"
                     /translation="MNDTLLADEFALFREAAKGTKKLTQNTFVPKAEPRKKLQELREL
                     KEKADTEFYFSDEYEPLLKEENEKVKYLREDVDPYILKQLRRGDFQPELFLDLHGLTR
                     EKAKKELAALILACEREKVYCASVMTGFGTRTLKDQIPRWLVQHPKVMALHQAPKEWG
                     GDASILILVEQPENPEKMFGR"
     gene            3797..4282
                     /locus_tag="PWY43_001174"
     CDS             3797..4282
                     /locus_tag="PWY43_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL2136059.1"
                     /translation="MSEQTFHQITALLDSHRARYRVVEHPSAGKSEEVAKIRGTEIGQ
                     GAKALVCKVKGNGVKQAVLAILPADYQADLTKIAEHLGGLRASLASPAEVAELTDCVF
                     GAIPPFSFHPDLLLVAEPSLFERYAELAFNAGTLERSIILNTEDYQKIVNPVLVQFAK
                     S"
     gene            complement(4284..5033)
                     /locus_tag="PWY43_001175"
     CDS             complement(4284..5033)
                     /locus_tag="PWY43_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2136060.1"
                     /translation="MEKHFEINEQGLSIRCKLFYEKELQQIENVVIVLHGFGSSKDLK
                     SNTKFGERLSSKYKGYAALAFDFPCHGDDARKKLSVEESITYLQLVIDYAKTSLNAKN
                     LYAYGTSFGGYLTLKYLAEKGNPFRKIALRAPAVQIYHSLMNRLSEEERQKLAKGREI
                     LWGFDRQMKISQAFFDELTDITQNDYLDYAEQILILHGTEDEMVALSTSQQFADNNVI
                     ELLPIEGADHAFSNPKLMDIAIGKIVEFFAP"
     gene            5205..6068
                     /locus_tag="PWY43_001176"
     CDS             5205..6068
                     /locus_tag="PWY43_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HDL2136061.1"
                     /translation="MLQLILAILCSVTVGVLIKFARQKGIRIDQSIAVNYIVTTTMTF
                     LLLKPDYQGQSITDIIANNPTSYIFVILSITLPTIFLVQAKALEFAGIIRTDAAQRLS
                     LFLPILAAFTIFGEQVTQNKLIALLLAFVALGCLLWKGNQGMEKGGKIAIISLALVWV
                     GYGVNDILFKQIAKSGSAFPVTLSIAFIGAGCFMFIYLLLKRTQWHVPSVLTGLLLGV
                     LNFGNILFYIQAHQAMKDDPTLVFTGMNLGVICLGTFVGAFFFKEKIHKVNYLGVAIA
                     IVAIICLFYWR"
     gene            6231..7754
                     /locus_tag="PWY43_001177"
                     /pseudo
     CDS             6231..7754
                     /locus_tag="PWY43_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            7809..9659
                     /locus_tag="PWY43_001178"
     CDS             7809..9659
                     /locus_tag="PWY43_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2136062.1"
                     /translation="MQVKKFSQKYINWVLKLGRLKASLLGFFMVALSAILIQSSLTYL
                     FIGRVVVVDILRSIAFGLISAPFVLYFFTLLVEKLEISRLKLEKAVRDLEELREQDAY
                     LNAKLEKNSRDKSILMATISHELRTPLNGIIGLSQILLDDKTTEKQQEYLKTINMSAT
                     ALAHIFGDIIDLEKIDSSRIELVRKEVEFEQLIHDIANFGHFMAGNKQINFEVKYPPD
                     LPKYIYIDHARLSQILWNLISNAVKFTPASGRILLEIYQLDKEQFSFAVTDSGIGIPE
                     KDQRKIFDMFYQAENSKEKKAQGSGIGLSVSKRIAKLMGGDLVVASQENQGSTFTLTV
                     QAESTENKPTFAIQQHSLKVLLVEDIEMNVVVARAMLEKIGCEVDVAMSGEETYQHFA
                     QDSYDLILLDIQLPDTTGFEIAQQLRLQYENEEIDYLPLLVALTANVIQSRQEYQAQG
                     MDDVLHKPLSLDELMRCLNQHFGDDFLQKNEEKQPLVERGSENLPYSITILNELIGVM
                     GKENVLANMDLFADLMPTYLANLQRYLSDWQTTNSVESRKLTADEAHKIKGALASVGL
                     HQLQQTAQLAQSDNGEQWEQNIAHWVEMICSKWQMDLQLAKEYVRSKGDI"
     gene            complement(9755..10309)
                     /locus_tag="PWY43_001179"
     CDS             complement(9755..10309)
                     /locus_tag="PWY43_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL2136063.1"
                     /translation="MKSQIYYDYYPSPIGQLLLLATEEGLIYIELGQEQQTTSLDNFL
                     SAQAASGQILEIFCKTKEILDRYFAGEPLDFQQLDFLAPQGTPFQQSVWKILREIPYG
                     QTTTYGEIATQLGKPSAMRAVGGAVGRNPISILVPCHRVLGKNQALTGFGGGLPNKRF
                     LLQLEDITYKDKGIEFVKPKTKKW"
     gene            complement(10376..11857)
                     /locus_tag="PWY43_001180"
     CDS             complement(10376..11857)
                     /locus_tag="PWY43_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HDL2136064.1"
                     /translation="MKMKNFSKTLIASLVAFSSAGASAAAFQLAEISTSGLGRAYAGN
                     AAVADNASVVATNPALMTLFKQTEISAGGIFVDADVNIDGKLNATGADASHKNVIPTA
                     IVPNAYIVSPINERLSIGGGVNVNYGLISEFDPNYSAGFLGGRTSLNAVNVNFSAAYD
                     LGYGFVLGAGLNAVHAKAELERFLGAGAARFAPAKQFLEDKKVQVQSAIQGLKALEAG
                     IAAGNTALEAQKTQVLAQVNGLLKSQNLNQTITNSAQLERLVNGIQNTNASSTIHKLK
                     GDKWALGWNVGLVYNINERNRLGFTYHSEIDLKFKGKYSNTVPVVIPKMVDTITGGAE
                     MDGTLRLRLPAFWEVSGYHKLTDKLAAQYSYKRTDWSKFQSLEAYDMQGNRLLHKTEN
                     FNDSQRIALGLSYDVNEILTLRTGVAFDESASVTHPSISIPDTDRAWYSVGATVRFTP
                     NLSADVGYSHLRGSNLTFSEDGLATFKSKARANLYGLNVNYKF"
     gene            12204..12656
                     /locus_tag="PWY43_001181"
     CDS             12204..12656
                     /locus_tag="PWY43_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL2136065.1"
                     /translation="MKLTSKGRYAVTAILDIALHGKLEPVSLADISDRQAISLSYLEQ
                     LFAQLRRDGIVQSVRGPGGGYQLGKKPEEISLGMIISAVNENIDVTKCKGSGNCRKDS
                     QCLTHSLWERLEDQIEQFLGTISVADLAAEHADHDCEKEHCHDHPHHH"
     gene            12711..13931
                     /locus_tag="PWY43_001182"
     CDS             12711..13931
                     /locus_tag="PWY43_001182"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HDL2136066.1"
                     /translation="MKLPIYLDYAATTPMDERVAKKMMEYMTKDGIFGNPASRSHKFG
                     WEAEEAVDVARNQIADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKT
                     EHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLKAAIRPDTILVSVMHVNNEMGVI
                     QPIKEIGAICRAKKIIFHVDATQSVGKIEVNVQDLNVDLMSFSSHKLYGPKGIGGLYV
                     CRKPRVRLEAIIHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMARLTVLRDR
                     LYNGFKDIEEVYVNGSMEEGKRIGSNLNISFNFVEGESLMMSLRDIAVSSGSACTSAS
                     LEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDHTIEIVKKAIVKLRELSPLWEMF
                     KEGVDLSKIEWNHH"
     gene            14058..14441
                     /gene="iscU"
                     /locus_tag="PWY43_001183"
     CDS             14058..14441
                     /gene="iscU"
                     /locus_tag="PWY43_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDL2136067.1"
                     /translation="MAYSEKVIDHYENPRNVGTFDKEASDTGTGMVGAPACGDILRLQ
                     IKVNDQGIIEDARFKAYGCGSAIASSSLITEWVKGKSLEEAGAIKNSDIAEELELPPV
                     KVHCSILAEDAIKAAIADYKEKQAK"
     gene            14550..14873
                     /gene="iscA"
                     /locus_tag="PWY43_001184"
     CDS             14550..14873
                     /gene="iscA"
                     /locus_tag="PWY43_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDL2136068.1"
                     /translation="MSITLTEAAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEF
                     VDVLNEDDQVFEQHGVKVIVDEKSLTYLNGTQLDFVKEGLNEGFKYTNPNVKNECGCG
                     ESFSV"
     gene            14883..15404
                     /gene="hscB"
                     /locus_tag="PWY43_001185"
     CDS             14883..15404
                     /gene="hscB"
                     /locus_tag="PWY43_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HDL2136069.1"
                     /translation="MNNPFTLFDLPVQFQLDNAQLSERYLALQKQLHPDNFAHKSSAE
                     QLEAVQKSADVNEALNILKDPILRAEAMIEIHTGEAKNLEEKSMRDVDFLMQQLEWHE
                     KLETIEHRKDETELTVFLKQIKAEQKAVLEQLEQTLNTADWQKANALTDKLRYFKKLI
                     IQIEKVEEKFFDY"
     gene            15458..16090
                     /locus_tag="PWY43_001186"
     CDS             15458..16090
                     /locus_tag="PWY43_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HDL2136070.1"
                     /translation="MKNLEQLLDKQSAWLILRDWFTQAKNHYEILASYPEDAGSALVG
                     MQLSTRSPLGAMVYETGGVFIDHGWLRILGSGNEKLPRGLFDWNFGKTFKQSGEQPSH
                     LLVADDAIGGYFAINAGGIGSDLGKVYYYHPKAKKWENTQLGYSEFLGWALAGDLNHF
                     YQDLRWENWQTDVEKLQGHQVLDLTSKIAVPIERHYQGEFAPENMGYSVA"
     gene            16238..16579
                     /locus_tag="PWY43_001187"
     CDS             16238..16579
                     /locus_tag="PWY43_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HDL2136071.1"
                     /translation="MKAILCQRAKQLRTNMTEAEKVLWQALRAKRFLAIKFKRQQVIG
                     NYIVDFVCFSAKLVIELDGSQHLDQQNYDNLRTHFLNGQGFRVLRFWNNQIHSDLEAV
                     LDTIFYEITRG"
BASE COUNT         5257 a         3037 c         3634 g         4807 t
ORIGIN      
        1 tacaaaaata aattgttcat ttagtattcc ttttaatgca ccaaatttaa agcgttcgca
       61 ttgcccattt gcacattcat gtgttttggg tgcggtttta aacataacga atagcataat
      121 aatttcaatg cagcagctaa tccaaataag ccattctgct ttacggtaga aaataaatgg
      181 aatagtaagt aatggtgcta ccatcgctga caggcttgat accttcagaa atttaccttg
      241 agccttagtt ttgagttggt aactatctcc ggtatcttga atattggcta ataagaatgc
      301 tttcgcattg gtactaaata aggtgctacc gagcccgaag aaaaaggtag caagtaatag
      361 cagagggtaa ttttttgttg tcagtaataa aatgtaagct agggcatcga gcaaacagcc
      421 aagcatcatg atagggtaac gtccaaaact atcaccccaa gaacctgcaa aaagggaaaa
      481 tgcttggctg gtaaagacca acaaagaaaa tgcagttgca atttggctgg tttcaaagcc
      541 tttgtagaat tgtaagtaga taaaaaatac gctttgcatt gcacataagc aagaccggaa
      601 aagaatttcc gccataaaat gatattttgg atattcatag ataatattta atgagaaaaa
      661 tgatttctaa tataactaaa tttaataagg aagcacaatg agaaaaatca aattattgca
      721 tgttatttta tctttaatat tagggctttc tgcggcagcg attggaagtt atccggcaat
      781 aaaagagatt ggcgaggagc gggcaaaaaa acatcaagat atattagatt taaatagaga
      841 actttatagt gcaatggtga accgtgattt ttcggctttg caaacattac taagcgatga
      901 ttttgagctt tattcatcaa caggtgaagt gattgataag caagaatgga tcaaattaat
      961 ccaaaagaga gtattggaat ttgataaggt tgatttgcta tctaccgatt ttcaggggaa
     1021 tcaatcatct ggattattaa gagtaaatgg tgagtttttt gggatagagc gggataatgt
     1081 ggtggtagaa atgaatattc gaaccattgt gagaaataca gaacgccaaa ttaaatgtat
     1141 tgtgattaaa gaggtttaag tgtcgcaact attgaatgcc ttagggcaga tgattaccga
     1201 acaacgtaat ccgaattcga tgaatattga ccgcctttca gcgttagata tagtccaagt
     1261 catcaaccaa gaagacaaac aagtagcgat tgcggtggag cagtgcctgc ctcaaattgc
     1321 acaagcggtg gaaaaaattg tgcaagcctt tgagaaaggt ggacgattgg tttatgtcgg
     1381 tgccggcacg agcggaagat taggggtgct tgatgcttcg gaatgtccgc ccacttatgg
     1441 cgtaaaacct gaaatggtgg ttggattaat tgccggtggt gagcgggcat tacgtttccc
     1501 gattgaaggg gcggaagata atcgtgagca aggcaaagta gatttacaag cggtcaattt
     1561 tagcgaaaaa gatgtattgg tcgggattgc agccagtggg cgaacgcctt atgtgttagg
     1621 ggcgatggaa tatgctaagc aacttggtgc gaccacggtg tcgattgcca gtaacccaaa
     1681 ttcggcaatg gcacaaattg cggatattgc gattgaaacc gttgtcgggc cggaagtgct
     1741 aaccggttcc agccgtatga aatccggcac agctcaaaaa ttggtattga atatgctcac
     1801 cactgcgtcg atgattttaa tcggtaaatg ctaccaaaat ttaatggtag atgtgcaagc
     1861 aactaatgaa aaactcaaag ctagagcaat tcggattgtg atgcaagcga cagaatgtga
     1921 acgagaagtg gctgaagata cactaaaatt agctgaaaat aacgctaaat tggcaattat
     1981 gatgatttta ggtaacttag ataagcagaa agccgaagat ttattaagcc aaaatcaagg
     2041 gcgattacaa aaagcgttaa aatagaagta tgtctgctct ctccccttgt gggagagaga
     2101 cagatcaatc attcattcag aatgattgat cagagagagg ggaggcagat ttaagcaaat
     2161 aaatggcgat gtttcactag ttcttctttg gttaaactga acacccctaa gccaccgtgt
     2221 tcaaattcca gccagttaaa cggcacactg gggaattgtt cgattaaatg caccatactg
     2281 ttgccgactt cgcagactag cacgccatca tcatttaagt aatcagcggc ttgagcaaga
     2341 atccgcttgg ttaaatctaa cccatctttg cccgagccaa gagctaatgc cggctcgtga
     2401 tggaactctt cgggcatatc gcctaagtct tcttcatcaa cataaggcgg attagtcaca
     2461 attaagtcgt atttatcttg cggtaaattg ttaaacagat cggaacagat cgggaaaact
     2521 cggtgagaca cctgatgacg ctcaatatta atttgagcaa cgtctaaggc atcaagggaa
     2581 agatcaaccg catcgacttc tgcttccgga aaacgctcgg cacaggcaat cgcaatacaa
     2641 ccgctgccgg tacacatatc taaaattcga tgtggctcgt ttttgataat gcctttaaac
     2701 ccttttttga tcagctcacc aatcggtgaa cgtggcacga tcacacgctc atcgacataa
     2761 tattcaccgc cacaaaacca agccgagttg gtaaggtatg ctacaggttt gcgagagcct
     2821 aaacgctcac agattaaatt gatgatacgt tctttctcaa ctcgagttaa gcgagaggaa
     2881 taaagtgttt ccggtacatc aaacggcaaa taaagccctg ccaacactaa ttgttgtgcc
     2941 tcgtcccacg cattatcgta gccgtgcccg taataaagat cagaggcatt aaaatagcta
     3001 tacccccaac gcatcatatc ttgaatagtg actaaatcat tcacaatggg cagggcggaa
     3061 atttcatcaa gaagtgcggt attatattta aacattggcg tgcctttttt cgtattgtta
     3121 aaatggctgt gttataccac attttgcttt gtgttagaat gtaaaaatga ataagaagga
     3181 aaagataatg aacgatacgt tattagccga tgaatttgca ttattcagag aggcagcaaa
     3241 aggcacgaaa aagctgacac aaaatacctt tgtgccgaaa gcggaacctc gcaagaaatt
     3301 gcaagaattg cgagaattaa aagaaaaagc tgatacagaa ttttatttct cggatgaata
     3361 tgagcctttg ctgaaagaag aaaatgaaaa agtgaaatat ctgcgagaag atgttgatcc
     3421 ttatattctc aaacagttac ggcgaggcga tttccaaccc gagctgtttt tagatctgca
     3481 tggtttaact cgagaaaaag cgaagaaaga gttggctgcg ttgattttag cctgtgagcg
     3541 ggaaaaggta tattgtgcca gtgtgatgac cggctttggc actcgcacgc tcaaagatca
     3601 aattccacgc tggttggttc agcacccaaa agtaatggcg ttgcaccagg ccccgaaaga
     3661 gtggggcgga gatgcctcta ttctaatttt ggttgagcag cctgaaaatc ctgaaaaaat
     3721 gtttgggcgt taaaattcgg ctgaattgcc aattttttat taaaaacgac cgcttgtgta
     3781 tcattaagga gaaatgatgt cagaacaaac tttccaccaa attacagcat tattagattc
     3841 gcaccgagct cgctatcgag tggtagaaca tccttctgcc gggaaatcgg aagaagtggc
     3901 aaaaattcga ggaacagaaa tcgggcaagg ggcgaaagcc ttagtttgca aggtaaaagg
     3961 caatggggta aagcaagctg tgcttgctat attacctgct gattaccaag cggatttaac
     4021 aaaaattgca gagcatcttg gtgggttaag agcctcactt gccagcccgg ctgaagtggc
     4081 agaattgacc gattgtgtat ttggggcaat tccgccgttt agctttcacc ccgatttatt
     4141 attggtggct gagccaagtc tgtttgagcg ttatgctgaa ttagccttta atgccggtac
     4201 gttggaacgt tcgattattc tgaataccga ggattaccaa aagatagtta atcctgtgtt
     4261 ggttcaattc gccaaaagct aggttatggg gcgaaaaact ccacgatttt gccaatagcg
     4321 atatccatta atttagggtt agaaaaagcg tgatctgccc cttcaatcgg gagcagttca
     4381 atcacgttat tatcggcaaa ctgctgtgaa gtgcttaacg ccaccatttc atcttccgtg
     4441 ccgtgcagaa ttaaaatttg ctcggcataa tccaaataat cgttttgggt aatatcggtt
     4501 agctcatcaa aaaaggcttg gctgattttc atctggcgat caaaccccca caaaatttct
     4561 cgccctttgg caagtttttg ccgttcctct tctgacaagc ggttcattaa cgaatggtag
     4621 atttgtactg ccggtgcccg caatgcaatt ttccgaaacg gattgccttt ttccgccaga
     4681 tatttcaagg ttaaatagcc gccaaaacta gtgccgtagg catacagatt tttagcattc
     4741 aggctggttt tggcataatc aatcaccagt tgcagataag taatggattc ctcaaccgag
     4801 agttttttac gggcatcatc accgtggcaa gggaaatcaa aggcaagggc agcgtagcct
     4861 ttatattttg agctaaggcg ttccccaaat ttggtattcg atttcagatc tttagacgaa
     4921 ccaaagccgt gcaacacaat cactacattc tcgatctgct gcaattcttt ttcataaaac
     4981 agtttacagc gaatgctgag accctgttca ttaatctcaa agtgtttttc cattttatgt
     5041 taagtattga aattgaagat gacaaaattt tagcactatt tgagtgcaag cggtcggatc
     5101 tctcgcaaaa tttgcaaatt ttccggtaaa tttaaccgct tgtactagcc aaaatttaag
     5161 catatttata gaatacagcc ttcgttttta aaaggagaaa tgaaatgtta caacttattt
     5221 tagcgatttt atgtagcgtg actgtaggcg tgttgattaa attcgcccgc caaaaaggga
     5281 ttcgtatcga ccaaagtatt gcggtgaact atatcgtgac taccacgatg acgtttttat
     5341 tactaaaacc ggattaccaa gggcagtcga ttaccgatat tattgctaat aacccgactt
     5401 cctatatttt tgtgatttta agtatcacat tgcctactat tttcttggtg caagcaaaag
     5461 ccctagagtt tgccggtatt atccgaaccg atgcggcaca acgtttatcg ctgttcctgc
     5521 cgattttggc ggcattcacc atttttggtg agcaggttac gcaaaataaa ttaattgcac
     5581 ttctgttagc tttcgtggca ctcggttgct tattatggaa aggcaatcag ggaatggaaa
     5641 aaggcggaaa aattgcgatt atcagccttg ctctggtttg ggtaggctat ggcgtgaacg
     5701 atattttatt taaacagatt gccaaatcag gctctgcttt ccctgtcacc ctttcgattg
     5761 cctttatcgg tgcaggctgt tttatgttta tctatttgct acttaaacgc actcaatggc
     5821 acgttccaag tgtcttaacc ggcttattgc taggcgtgtt gaacttcggc aatattctgt
     5881 tctatattca agctcaccaa gccatgaaag atgacccaac tttagtcttc actggaatga
     5941 acttaggggt aatttgctta ggcactttcg ttggagcatt cttctttaaa gagaaaatcc
     6001 acaaagtgaa ctacttaggc gtggcgattg cgattgtggc gattatttgc ttgttttatt
     6061 ggcggtaaat atactttcca aaattaaggg cgaacattat gttcgccctt aatttttgag
     6121 taatatagtg taaaatattc aataaattcg accgcttgtt gtaccgattc atatttcata
     6181 aaggacaaaa aatgaagcta aaagccttat ctacactcat tgcctcttca atgctatata
     6241 gtcagagttt tgctgtaccg ccggaaaaaa cagctgaaga gccacagccc tttttcaata
     6301 aaaccgaatc tgggtatgat ttagtcttta atgatcaaaa ctatcgttta attgaaacgg
     6361 aattaaatgg cgagaaaatc caattccgtg cctttgagcg gattgtgtat gtgaacaatc
     6421 cgattgagcc agattaccaa acaattaatc tgtatgtgcc ggaagcttac tacaataatg
     6481 gcgaaatcaa tggctaccaa gcggataccg ccccgatctt cttgccaaat tcagtggggg
     6541 ctatatgccg gcaaaagcgg caagttatga taaaaaaaga cgtgacggtt caccaggtac
     6601 gattttggtg gcgttatcta aaggctatgt ggtggcaagt gtgggagcaa gaggacgcac
     6661 gctacaaaaa gagggaaaat acaccggcaa agcacctttt gccattattg atttaaaatc
     6721 agcagtgcgg tatctgcatg ctaatgatga aaaaatgccg ggcgatgcaa ataaaattat
     6781 ctctaacggc accagtgccg gaggagcatt atctactttg ttaggagctt cagctgatca
     6841 ttacgactat gagccttatt tgaaagaggc gggggcattg aatgccagtg acaaaatttt
     6901 tgcagtttct gcctattgcc caattaccaa ccttgaaaat gcggatatgg cgtatgaatg
     6961 gcaatttaat ggtgtcaatg aatatagccg aatcgatatg agtcgattaa atgcagcaga
     7021 gtttaacgac cgctcaaaac caaagccgaa aattgaaggc tcgttaaatg aggcggaaat
     7081 taaagtttct aatgaattag cagaacgctt tccgacttat ttgaacagtt tgcatttggt
     7141 tgatgagaaa ggcaatcctt taacacttga tccaaaaggc aacggctcgt ttaaagatta
     7201 tttatctgaa gtggtaaaaa cggctgcaaa taaagcctat agaggtttag ttcaagatag
     7261 cgaagagcaa aaagcctttc aacaaattag ttggctttca tttgagaaag gcaaagtcag
     7321 cagtgtggac tggtttggtt atgtgttttc agataaacgg atgaaatcac cacctgcatt
     7381 tgatgcgtta aatggaagtt cgggcgagaa taatttattt ggcacagaca cggaaaataa
     7441 ccgccatttt acgctttata gtgcggaacg ttctgccaat aaagatctca atttagccga
     7501 tccgcaaatc gtgaaacgga tgaacccgat gcactatttg gataaccgca aatgctgcgg
     7561 aacattggcg gattagggtg ggaacggcag atagagatac ctcgctggca atttctgcaa
     7621 tcttagcaat aaaactgcaa atggcaggca aaaatgttaa ttatgaaacc ccgtggaatg
     7681 taccccattc aggtgattat gatgtaaatg aattattcct atgggctgat gagctagtca
     7741 aaggcaaaaa gtaaaaaata gagtaaaata aagcagatct gatggtctgt tttttgtttt
     7801 gaggtcgtat gcaagttaaa aaatttagcc aaaaatatat taattgggta ttaaagctag
     7861 ggcggttaaa agcttctctt ttaggctttt ttatggtcgc tctttcggca attttaattc
     7921 aaagtagctt aacctatctt tttattgggc gagtagtcgt ggtggatatt ttacgctcca
     7981 tcgcttttgg tttaatttct gcgccttttg tgctttattt cttcacgtta ttggtggaaa
     8041 aacttgaaat atctcgcttg aaattagaaa aagcagtaag agatttggaa gaactccggg
     8101 aacaagatgc ctatcttaac gctaaattag aaaaaaacag ccgtgataaa agcattttaa
     8161 tggcgaccat tagtcacgaa cttcgtactc cccttaatgg aattatcggc ttgagccaaa
     8221 ttttgttaga tgataaaacc accgaaaaac agcaagaata tctaaaaact atcaatatga
     8281 gtgccactgc attggctcat atttttggcg atattattga tttagaaaaa attgatagta
     8341 gccgaattga attggtaaga aaagaggtgg aatttgaaca actcattcac gatattgcta
     8401 attttgggca ttttatggcg ggcaataagc agattaattt tgaagtgaag tatccgcctg
     8461 atttgccgaa gtatatctat atcgaccacg cacgcttgag ccaaatttta tggaatttga
     8521 tcagcaatgc ggtgaaattt acccctgcaa gcggtcgaat tttgctggaa atttaccaat
     8581 tagataaaga acagtttagc tttgcggtga cggattcagg catcggtatt ccggaaaaag
     8641 atcaacgcaa aatttttgat atgttctacc aagcggaaaa ctctaaagag aaaaaagcgc
     8701 aaggtagcgg gattggctta tcggtctcga aacgcattgc gaagttaatg ggcggtgatt
     8761 tggtggtggc aagccaagaa aatcaaggct caacttttac cctcaccgtg caggcggaaa
     8821 gcaccgaaaa caagccgaca tttgctattc agcagcattc ccttaaggtg ttattggtgg
     8881 aagatatcga aatgaatgtg gtggttgctc gagcaatgtt ggaaaaaatc gggtgtgagg
     8941 ttgatgtggc aatgagtggt gaagaaacgt accaacattt tgcacaggac agctacgatc
     9001 tgattttact tgatattcaa ctgccggata ccaccggttt tgaaattgca cagcaattaa
     9061 gattgcaata tgaaaacgag gaaattgact atttaccgct gttggttgct ttgacggcaa
     9121 atgtgatcca aagccggcag gagtaccaag cacaaggtat ggacgatgtg ctacacaaac
     9181 cgctctcttt agatgaatta atgcgttgtc tcaaccaaca ttttggagat gattttttgc
     9241 aaaaaaatga agaaaaacag ccgcttgtag agagaggttc tgagaattta ccttatagca
     9301 ttacaattct taatgaattg attggggtaa tggggaaaga gaatgtgctt gccaatatgg
     9361 atttatttgc tgatttaatg cccacttatt tggcaaattt acagcgttat ttaagtgatt
     9421 ggcaaaccac aaattctgtg gaaagccgta aattaacggc ggacgaggct cataagatta
     9481 agggagcatt agcatcagtc ggattacatc agctacaaca aactgctcag cttgctcaat
     9541 ccgataacgg cgagcagtgg gagcaaaata ttgcacactg ggtggaaatg atttgttcca
     9601 aatggcagat ggatttgcaa ttagctaagg aatatgttag aagcaagggg gacatttagt
     9661 aaatttgaaa tgttacgatg gtgcaagcgt ccacgcttgt accaatttta atatctgaag
     9721 cacaagcgtg gacgcttgtg ctatcaaaac gtatttacca tttcttggtt ttcggtttta
     9781 caaattcaat gcctttatct ttataggtaa tatcttccag ctgtaataga aaacgcttat
     9841 tcggcaagcc accaccgaat ccggttaagg cttgattttt ccctaatacc ctatggcaag
     9901 gcactaaaat cgaaatcggg tttcgcccaa ctgcaccgcc gacggctcgc atcgcacttg
     9961 gcttgcctag ttgggtggca atttcgccat aagtagtagt ttgcccgtaa gggatttcac
    10021 gcaaaatttt ccaaactgat tgctggaatg gagtgccttg cggggcaaga aaatccaact
    10081 gctgaaaatc caatggctcg ccggcaaaat agcggtctaa aatttctttg gttttgcaaa
    10141 aaatttctaa aatctgacca cttgcagctt gagcggaaag gaaattatca agcgaggtgg
    10201 tttgttgctc ttgccctagc tcaatgtaaa tcaagccttc ttctgtggct aaaagcaata
    10261 attgcccgat agggcttggg tagtaatcgt aataaatttg agatttcatg tttttatgcc
    10321 aataaaaaaa gaggtgtaat tgattttaca cctctttaaa ccttaattca tcaaattaga
    10381 atttgtagtt tacgtttaaa ccgtaaagat ttgcacgagc tttgctcttg aatgtggcta
    10441 aaccgtcttc agagaaggtc aggttgctac cacgaagatg tgagtaacct acatcagcag
    10501 ataggtttgg cgtgaaacgc acggttgcac ctacagaata ccaagcacga tcagtgtccg
    10561 gaattgagat tgatggatga gttacacttg cactttcatc aaatgctaca cctgtacgca
    10621 atgttagtat ttcattcaca tcataagaca agcctaatgc aatacgttga gagtcattaa
    10681 agttctcggt tttgtgcaat aggcggttac cttgcatatc ataagcttct aggctttgga
    10741 atttgctcca gtctgtgcgt ttatagctgt attgtgcagc aagtttatcg gttagtttgt
    10801 ggtaacctga cacttcccag aatgccggta ggcgtaatct tagtgtacca tccatctctg
    10861 caccgccggt aatggtatct accatcttag gaataactac aggaacggta ttagaatatt
    10921 tgcctttaaa tttaagatca atttcagagt ggtaagtaaa acctaaacgg tttctttcat
    10981 tgatattata aaccaagcca acattccaac ccaatgccca tttatcgcct ttaagtttat
    11041 gaattgttga acttgcattg gtattttgaa tgccattaac caaacgttct aattgagcag
    11101 aattcgtgat ggtttggttt aaattttgag attttaataa accgtttact tgagctaata
    11161 cttgtgtttt ttgagcttca agagcggtat tacctgctgc gatacctgcc tcaagagctt
    11221 ttaaaccttg aattgcactt tgtacttgta ctttcttgtc ttccaaaaat tgtttagctg
    11281 gagcaaaacg tgccgcaccg gcacctaaga aacgttctaa ttctgctttt gcgtgtactg
    11341 cattcaaacc tgcacctaat acaaaaccat aacctaaatc ataagccgca ctaaaattaa
    11401 cattcacagc atttaatgat gtacgaccac ctaagaaacc agcagaatag ttagggtcaa
    11461 attcagatat caaaccataa tttacattta cgccaccacc aatagaaagg cgctcattga
    11521 taggtgaaac gatataagca tttggaacaa tagcggtagg aatgacattt ttatgtgagg
    11581 catctgcacc tgtagcattg agctttccat caatatttac atcagcatct acgaaaatac
    11641 caccggcaga aatttcggtt tgtttaaata acgtcattaa cgcagggttg gttgcaacaa
    11701 cagaagcgtt atcggctaca gcggcattac ctgcgtaagc tctacctaaa ccggatgttg
    11761 aaatttcagc taattggaat gcggcagcag atgcaccggc tgagctgaat gctactaatg
    11821 aagcgattaa agttttgcta aagtttttca ttttcatagg aaatctctta aaaaatatat
    11881 attttagaat gaataattca taaaagttat aagaagtaag aagaaagtgc agattttatg
    11941 tgtcgaagta ctctctatca attaaatttc aactgttccg atcagttagg agcaaataag
    12001 agtgaaattg tgcggtctat gaactgttta tctttggtat tttcggctct tttttcgtat
    12061 aattagcgtt attgttttaa tctgttttaa tatataaaac ctttcacact aattagtcct
    12121 aaatgattga ctaaattggt ggggtatatc aaaattgagc aactgttcca gaagattttc
    12181 tggataatca atgaggatat tttatgaagc ttacctcaaa agggcgttat gcagtaaccg
    12241 ccattttaga tattgcttta cacggaaaac tagagccggt tagcttagcc gatatttcag
    12301 atcgccaagc gatttcactg tcttatttag agcaactgtt tgctcaactt cgccgtgacg
    12361 gtatcgtgca gagcgttcgt ggaccgggtg gtgggtatca attaggtaaa aaaccggaag
    12421 agattagctt gggtatgatc atctccgccg tgaatgaaaa tattgatgtc accaaatgta
    12481 aaggtagtgg taattgccgt aaagattctc agtgtttaac gcattctttg tgggagcggt
    12541 tagaagatca aattgagcag tttttaggga caatttcagt tgcggattta gccgcagaac
    12601 acgctgatca cgattgtgaa aaagagcatt gtcacgatca tcctcatcat cactaattgc
    12661 aaatttttta tcaaaaatga ccgcttgggt tgttttaact aggagtaaca atgaaattac
    12721 caatttattt ggattacgca gcaaccacac caatggacga acgtgttgcg aaaaaaatga
    12781 tggaatatat gaccaaagac ggtatctttg gtaaccctgc ttctcgctca cacaaatttg
    12841 gttgggaagc ggaagaagcg gttgatgtgg ctcgtaacca aattgccgat ttaatcggtg
    12901 cagacagccg tgaaatcgtt ttcacttctg gtgcaacaga gtcggataac ttagccatca
    12961 aaggtgcggc tcatttctat caaaccaaag gtaaacacat tatcactgtt aaaaccgaac
    13021 ataaagcagt attagatact tgccgtcagt tagagcgtga aggttttgaa gtgacttact
    13081 tagaccctga agaagacggt attttagatt tagctaaatt aaaagcggct attcgtccgg
    13141 acacaatttt agtttcggta atgcacgtaa ataacgaaat gggcgtaatc caaccgatta
    13201 aagagatcgg tgcaatctgc cgtgcgaaga aaatcatttt ccacgttgat gcgacccagt
    13261 ctgttggtaa aatcgaagtg aatgtacaag acttaaatgt tgatttaatg tctttctcaa
    13321 gccacaaatt atacggacca aaaggtatcg gcggtctata tgtttgtcgt aaaccacgtg
    13381 tgcgtttaga agcaattatt cacggtggcg gtcacgagcg tggtatgcgt tcgggtacat
    13441 tgcctgttca ccaaattgta ggtatgggtg aagcatatcg tatcgctaaa gaagaaatgg
    13501 caacggaaat ggctcgttta accgtgttac gtgatcgttt atataacggc tttaaagata
    13561 ttgaagaagt atatgtaaac ggttcaatgg aagaaggcaa acgtatcggt tcaaacctaa
    13621 acatcagctt taactttgtt gagggcgaat cgttaatgat gtcattacgt gatatcgcag
    13681 tatcatcagg ttcagcttgt acttctgcaa gtttagagcc atcttatgtg ttacgtgcaa
    13741 tcggtcgtga cgatgaatta gctcacagct caatccgttt cactttaggt cgttggacga
    13801 ctgaagaaga aatcgatcat actattgaaa tcgtgaaaaa agctatcgtg aaattgcgtg
    13861 agctttcgcc tctttgggaa atgttcaaag aaggtgtaga tttgtctaaa atcgaatgga
    13921 atcaccacta attcagaaaa attttgcaag ggcaaataat atttgcccct acaaaataag
    13981 ctttcatcac aaatagtaaa ttgaatgtag gggcgaaata ggtttcgccg gaaataggaa
    14041 ataaaaggaa tacaaaaatg gcatatagcg aaaaagtaat tgatcattac gaaaacccac
    14101 gcaacgtagg gacttttgat aaagaagcat cagacaccgg tacaggtatg gtgggagcac
    14161 cggcttgtgg tgatatttta cgtttacaaa ttaaagtgaa cgatcaaggt atcattgaag
    14221 atgcacgctt taaagcatac ggttgtggtt ctgcaatcgc atcaagttct ctcattaccg
    14281 aatgggtaaa aggtaaatca ttagaggaag caggtgcgat taaaaacagt gatatcgctg
    14341 aagagttaga attaccaccg gtaaaagttc actgttcaat cttagcagaa gatgcaatca
    14401 aagctgcaat tgcagactac aaagagaaac aagctaagta atatgataca atcaatgcaa
    14461 gcggtcgatt tttgcaaaaa atttgcaaaa tttaaccgct tgcatttgct atttctatct
    14521 tgatttaatc cattcacata aggaaaacta tgagcattac cttaacagaa gctgccgcaa
    14581 accgagttcg tacattctta gaaaacagag gtaaaggtat cgggctaagg cttggtgtga
    14641 aaacttccgg ttgttcgggg cttgcctatg tgcttgaatt tgttgatgta ttaaatgaag
    14701 acgatcaggt tttcgaacaa cacggcgtaa aagttattgt cgatgaaaaa agcctgactt
    14761 acttaaacgg tacgcagctg gattttgtga aagaaggctt aaacgaaggc tttaaataca
    14821 ccaacccgaa tgttaaaaac gaatgcggct gtggcgaaag ttttagtgtg taaggaactg
    14881 taatgaataa cccttttact ttatttgatt tacccgttca atttcaactt gataacgccc
    14941 agctttctga gcgttatctt gctttacaga aacagcttca cccagataac tttgcccaca
    15001 aatcaagtgc tgaacagtta gaagcggtgc aaaaatctgc tgatgtcaat gaggcactca
    15061 atattcttaa agatccgatt ttacgggcag aagcgatgat tgaaatccat acaggtgaag
    15121 ccaaaaatct cgaagaaaaa agtatgcgag atgtggattt cttaatgcag caactcgaat
    15181 ggcacgaaaa actggaaacc attgagcatc gtaaagacga aaccgaactg accgtctttt
    15241 taaaacagat taaagcggaa caaaaagcag tattagaaca gcttgaacaa acgttaaata
    15301 cagccgactg gcaaaaagcc aatgcgttaa ccgacaagct ccgttacttt aaaaaattaa
    15361 ttattcagat tgagaaagta gaagaaaaat tctttgacta ttaatcctac atttgtatgg
    15421 ttttcgcaag cggtcaaatt ctaaggaaat tttgcaaatg aaaaatttag aacaattatt
    15481 agataaacaa tcggcttggt taattttaag agactggttt acccaagcca aaaatcatta
    15541 cgaaatttta gcgagttacc ctgaagatgc cggcagtgca ttggttggta tgcaactttc
    15601 tactcgctca ccgctagggg caatggtgta tgaaaccggt ggcgtattta ttgaccacgg
    15661 ttggttacgc attttaggct caggcaacga aaaattgcct cgaggcttat ttgattggaa
    15721 ctttggcaaa acttttaaac aatctggcga acaaccaagc cacttgctgg tagcggatga
    15781 tgcaatcggt ggctattttg ccattaatgc cggcggaatc ggctcagatc ttggcaaagt
    15841 ttattattat catccaaaag ctaaaaaatg ggaaaacacc caattaggtt attcagaatt
    15901 tctaggctgg gcattagccg gagatttaaa ccatttttat caggatttgc gttgggaaaa
    15961 ctggcaaacc gatgtagaga aattacaagg gcatcaagtg ctggatctta caagtaaaat
    16021 cgccgttccg attgaacgcc actaccaagg cgaatttgcc cctgaaaata tgggatattc
    16081 ggtggcttaa gtgtttgtaa gagaggctga aatataggct gtttagctta tttttttaga
    16141 acgaaagaaa ttttctgatt ttcgatttag atcacaaaaa tcgctcaaat tatatttttt
    16201 attcctttta tactaaatta aaaaagagga ataaaaaatg aaagcaattt tatgccaaag
    16261 agctaaacag cttcggacta atatgacaga agcagagaag gttctttggc aagcattaag
    16321 agcaaaacga tttttagcga taaaatttaa acggcaacaa gttataggta actatattgt
    16381 cgattttgtt tgcttttcag cgaaattagt tattgaatta gatggttcac aacatttaga
    16441 tcaacaaaac tatgataatc tacgaactca ttttttaaac ggtcaggggt tcagggtttt
    16501 aagattttgg aataatcaaa ttcatagcga tctagaggct gttttagata caatttttta
    16561 tgaaattacg agagggtaat tccccctctc tctgataaat cattctgaac gaatgattta
    16621 tctgtctctc tcccacaagg ggcgagagtt aattttgcgg ttattttaga attttgaaag
    16681 aacaacattt tctcctacaa aaggagaatg ctaatttttt agttgttttg aattt
//
LOCUS       DANKBV010000045        16682 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.045, whole genome shotgun sequence.
ACCESSION   DANKBV010000045 DANKBV010000000
VERSION     DANKBV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16682
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.045"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(417..1457)
                     /locus_tag="PWY43_001188"
     CDS             complement(417..1457)
                     /locus_tag="PWY43_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2136072.1"
                     /translation="MKKQLRKNLTQNDRLQPQAQTEIFSFGDPIPVLDRADILNYLEC
                     SAMYEKWYNPPMSFDGLAKSLRSSTHHESAIITKANILLSTCEVDSRYLSRRDLSSFV
                     KDYLVFGNAYFEVVRNRLGQVQRIESPLAKYVRKGVEAGQFYYVPQRFDHQEHEFAKG
                     SIYHLLEPDINQDIYGLPQYLSALQSAWLNESATLFRRKYFLNGAHAGFVFYMTENSA
                     KQGDVDNIREQLRKSKGVGNFKNLFVHSPGGNKDGIQIIPIADVSAKDEFFNIKNVSR
                     DDVLAAHRVPPQLMGIIPNNTGGFGNVADAAEVFFITEIEPLQERLKEFNQWLGQEVI
                     KFKPSKLLQRTQ"
     gene            complement(1466..3268)
                     /locus_tag="PWY43_001189"
     CDS             complement(1466..3268)
                     /locus_tag="PWY43_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDL2136073.1"
                     /translation="MENENTITEYEVLAANPLHDKREAQSKYWAGYTVTEISRQLNIP
                     VSTIASWKKREKWDEISPVGRVEATLEARLNLLIMKEVKTGSDYKEIDLLGRQLERVA
                     RVKKYANGGGNEADLNPNIKSRNKGDRKKPEQNAISEEQAELLINGFLDGMFHYQKKW
                     HEAGLTHRIRNILKSRQIGATYYFAHEALVDALVTGRNQIFISASKKQALQFRAYIVA
                     YAKRVADVELKGETITLPNESQLIFLGTNSKTAQSYHGNLYFDEIFWVNRFEEIRKVA
                     AGMASQKQYRITYFSTPSSITHSAYLLWSGKLFNRKRPKAEQVEIDISHANLKNGKKC
                     GDGQWRQIVNIYDAEAGGCNLFDIEQLKLENSPDEFEQLFMCEFIDDNQSVFKFTMIQ
                     RCLVDSMEIWKDYVFTDGYMRPFGNKEVWVGYDPSYTGDRSALVVIAPPKVDGGKFRL
                     LEYRTFKGADFAEQAAEIIAICAKYNVTRLAIDTTGLGVGVYEIVKKERPDAVALTYN
                     VELKSKMVLKGLDIISKGRFEFDSMHAVEVGASFMAIKKQITNSGRQVTYVADRSEEA
                     SHADLAWACLQVFINEPFDGKLEETTGTVDFLDD"
     gene            3418..4245
                     /locus_tag="PWY43_001190"
     CDS             3418..4245
                     /locus_tag="PWY43_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDL2136074.1"
                     /translation="MTTKSKWFVVATEGATTDGREISREWLTQIEENYDPKNNYGARI
                     NLEHFRWRWFEKDDPHSYSYGDVLAVKTEEREDGKLQLLAEIAPTEALVKLVKDKQKV
                     YTSVEIDLNFADTGEAYLVGLAVTDTPASLGTEYLTFCAGATHNPLADRKQKPENLVS
                     EAVEANLEFTAEPEKGAWLEKFKAMFTKAKTDNDGKFAEHEKAMELLAEQFSKVSTEN
                     DRLQTELSTLQTEFTELQKQAGEFAEKFAKLEQEPSANYTPRPKATGAKAGDVQTDF"
     gene            4259..5287
                     /locus_tag="PWY43_001191"
     CDS             4259..5287
                     /locus_tag="PWY43_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDL2136075.1"
                     /translation="MKDLTLEKYNAYLARQAELNNLPFNALATGIKFTVQPSVQQKLY
                     EKVRESSDFLKSISFVFVDEQTGETLGLDSAHTVASTTDTSGDGERKTTSIAKLVKQT
                     YHCQQINFDTHINYKQLDMWAKFPDFQQKVANVAAKQRKRDLIMIGFNGTSRAATSDR
                     NSNPLLQDVAKGWLQKMREDAKERVMNGESTDNQVLVGKGQEYANLDALVMDATEELI
                     DEWHRDDTDLVVITGRKLLADKYFPIVNQQNAPTEQLAADIVISQKRIGGLKAVRVPF
                     FPANAILITKLENLAIYVQEGTTRKHIENVPKKDRIETYESENIDYVVEDYGCAALIE
                     NITLKDKE"
     gene            5297..5986
                     /locus_tag="PWY43_001192"
     CDS             5297..5986
                     /locus_tag="PWY43_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDL2136076.1"
                     /translation="MRMSPARAHFLRKTAESESATPNRENLEGLQGYDLVLAQLKAHQ
                     RQLKQTQSIERKIEFKRNNFEQYRPWVEGSLAKGSGVQDTVITTMLIWSIDIGDYPTA
                     LAIAQYVLMHDLAMPEQFERTPATALVEELAEAAKKARDQKQPFEAAVLKQANDLTAE
                     FDMPDPVRAKLLRELGELIQEAEPQTALEHYQRAIALDPQCGAKGLRDKLEKQLAKQA
                     EEPKPAEEQNE"
     gene            6098..6613
                     /locus_tag="PWY43_001193"
     CDS             6098..6613
                     /locus_tag="PWY43_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDL2136077.1"
                     /translation="MEQRNHVISIPKVNGYESSKNAIKPTAESDETITNNGFFPNIEL
                     LEVRNAMRIDGTVINERLKQAVIEAMATVNADLKAYRLNAEQAQKANLQACDDEQING
                     ESVLVYKYKRAVYCLAVANLYERYRSYDSTKDGHDKAEELESTAGDLKRDYHFAVRDI
                     LGENRMISELI"
     gene            6610..6822
                     /locus_tag="PWY43_001194"
     CDS             6610..6822
                     /locus_tag="PWY43_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL2136078.1"
                     /translation="MNTLIARQDDTLDELIFRHYGQTAGLVEQALEYNPELAHLPRLP
                     IGTVVTMPDIESRFATVAKSSVQLWD"
     gene            6828..7034
                     /locus_tag="PWY43_001195"
     CDS             6828..7034
                     /locus_tag="PWY43_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HDL2136079.1"
                     /translation="MHKTEQASYFGAIFGFFGSLTLSDWGVVVSILVAILTLLLNWAY
                     RAKEYKLKEREFELKLQNQGKKNE"
     gene            7027..7593
                     /locus_tag="PWY43_001196"
     CDS             7027..7593
                     /locus_tag="PWY43_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL2136080.1"
                     /translation="MSKSVKIGVFVCSVAAIIGTVKQYYSSEIRTSETGLAIIGNAEG
                     CRRDPYKCPADVITVGIGSTEASGEKINVNHKYTDKEIAERWIKDLKIAERCINRYFN
                     GDKMNENQFSAMVSAAFNMGCYNLRFYPNENGKYIQTTIHKYAMAKDFKAMCNRIPDF
                     NRSGGKVLRGLKIRREKEKALCLGQEVK"
     gene            7590..8045
                     /locus_tag="PWY43_001197"
     CDS             7590..8045
                     /locus_tag="PWY43_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136081.1"
                     /translation="MIVKLCQMTNKHFSPLMIVLVLVGVLANFVQFRQNESLKADLQA
                     RTTQNQLIQADNRNLANQLENSQIQLEAYQKQVDDLNQKVLAKMKQAEQRSNEILNEL
                     EKHKKWADSAVPPSVGKLLNQRKRAVSHPQIEPDNLSEKAGVPNTGNRD"
     gene            8194..8415
                     /locus_tag="PWY43_001198"
     CDS             8194..8415
                     /locus_tag="PWY43_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDL2136082.1"
                     /translation="MSDQIDRANELAEKAREAALAKILQNQTACTSLFECEDCGEPIP
                     EKRREMVIGCTRCIECQTIYEHKQKGYRR"
     gene            8412..8897
                     /locus_tag="PWY43_001199"
     CDS             8412..8897
                     /locus_tag="PWY43_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2136083.1"
                     /translation="MIKPDRLRALLTQTIDIFQTNSENLILQYDKGKIKSKGSQSHSF
                     EYHYDLELIVVDFPYHPDVLFVPVLNFVRNEQWELLQNPELQDKIEFEIDHNNHESYD
                     IYIRIPLTERVIVKEQDGHLIATHADEPNLADVSPFTRLTEYEVYLKDELIYQWTEPN
                     E"
     gene            8890..9348
                     /locus_tag="PWY43_001200"
     CDS             8890..9348
                     /locus_tag="PWY43_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL2136084.1"
                     /translation="MSEPIEQVKTAFDHLLNNISKPRRRLMYQQIGRELARSQRRRIK
                     AQQNPDGSAYEPRKKPKKGVKSKIKSGKMFDKITQPRFMRLRLESEGVSLGYEGGDAV
                     IARIHQQGLIGRVRKDWDLKVKYASRELLGFTDDDLQMIEDYMINILAGS"
     gene            complement(9399..9677)
                     /locus_tag="PWY43_001201"
     CDS             complement(9399..9677)
                     /locus_tag="PWY43_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136085.1"
                     /translation="MEILFILLGLIGFGALLAIGFTTFAAIFGVVTTVFLSLLSLLAN
                     MVGLSIIVILISLYVLLPLNTFILIALIFSAILGFLCWYHSDRQSVNQ"
     gene            9716..12628
                     /locus_tag="PWY43_001202"
     CDS             9716..12628
                     /locus_tag="PWY43_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2136086.1"
                     /translation="MSNNLKIQVVLSALDKLTAPFKSVVKQTEKLSQSLKVQRDELKA
                     LEKTQSKMQSFKKMDDSIKKSNETILKQTKHLDNLKNKISQMKNERIELKVKMDAKKR
                     EFQQITKGGSAWSARAAFLDKDFEKMQREYEKLGASISSVSRKRTEENRALKDSRHQQ
                     AKQLLYFRSLRRELRASGINIKDFAQSETNLTDKIAKANQLIDQQKAKLEKLNAVKAR
                     NEKYWATVEKVKNTSERLHNLGQRSMISGAAISAPVVGLGKGVVGMAQTAGKFEQFNA
                     ILEVTEGSAEKAKQSFDWVKQFAVDTPSNLDEAMEAFVKLRAYGLDPTNGLLQTLGDT
                     GAAMGKPVMQAVEAIADALTGENERLKEFGIKGSVVKGTNIIEYAYTDKLGKQQVAKV
                     NKNNRKEIEQTLTRIFNEKYAGAMEKQAKTLVGIWAKIEDYWTNFQMQVMESGAFDWI
                     KNKLQWVLDSLDKMAENGELQKWAEDVGAVIQETVQGLWAFGEKIIEVVKWLASLARE
                     NKGLIATFVKWSAILGSSLTVLGAFAVVASFALYPVARLALGIGKFTGASTLLNKALF
                     DSNDKFRLFNKSLYSSGTTAAFVKSKFNQLATLPNVLGSSFNKLGGGIKMLIPQLKTK
                     VFWLNMLKGILGKLWSAIRFIFSPLKLLLGIFSPIGLAITAISVAGVALYRNWEKVRA
                     FFGGFLQGLSKGLAPVLERFKPLGKLFETISNWVKSTFKWLSDLLTPADKTSESLDKA
                     SSAGEKFGLALAGAIDLVTSPVQWLVDQFKWISENMPSWDTIGEGVKKAATAIVPTEH
                     AKQISQTAEMSNNMYDPNYTPPQLEQKWIGGLVGNGKGRGFATGGYTGNGGKYEPAGI
                     VHKGEYVMTKEATARLGVANLNRLNYGKVAGLTALASSVAFAQPMPAVKIDSRPPLTA
                     SQPSPTVAPVSQNIHITINATGGQDPQAIARLVAMELEKQQRQAQARARSSLRDRG"
     gene            12690..12869
                     /locus_tag="PWY43_001203"
     CDS             12690..12869
                     /locus_tag="PWY43_001203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136087.1"
                     /translation="MMFKEKGYDEFVNECVRKGREEHKAGLGFTLEQSRLHIKKTIEQ
                     KSQELNEFQREVVYG"
     gene            12862..13149
                     /locus_tag="PWY43_001204"
     CDS             12862..13149
                     /locus_tag="PWY43_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2136088.1"
                     /translation="MAKITIKPQALSQFDEIVKSVIEFTGFETSGIRLQSDIFDKIEQ
                     IAFMPLSGRAREDGTRETFVRRYRIVYDYDRQADEVIILAIIHSSRLYPRL"
     gene            13278..13883
                     /locus_tag="PWY43_001205"
     CDS             13278..13883
                     /locus_tag="PWY43_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL2136089.1"
                     /translation="MSEPNHAEQIRRLDNIIRLGTIAEVDLTTATARVNSGGITTDFL
                     PWLTFRAGTSQTWSAPTVGEQCVVLSVSGEFTTGVILFGIYTQNAPSQSADEHLFRFS
                     DGAEISYNTASGQLTVKNCKVVIVQATESITLDTPTVKATKNVVIGGNLSVKGTTSSQ
                     GAISTQGAVTAKGDVSGAGISLGSHHHIEQGDGRPTSGAKP"
     gene            13883..14218
                     /locus_tag="PWY43_001206"
     CDS             13883..14218
                     /locus_tag="PWY43_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL2136090.1"
                     /translation="MNRETGEQLEDEVEHIKQSIKDILLTAKGSRVMRRTYGSNLYKL
                     TDKPIAASLIMQLSAACVMALQQWEPRIAITRFKVEFAEDNHNKLICTLDFTLKHRKI
                     SIKGMRFKL"
     gene            14215..15132
                     /locus_tag="PWY43_001207"
     CDS             14215..15132
                     /locus_tag="PWY43_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDL2136091.1"
                     /translation="MSEIVDLSKLPAPKVLEELDYETLLAERKAKFLSLYPESERSVM
                     AARLALESEPITKLLEENCYLQLMERQRINSAAQATMLAYATGTDLDVIGGNYNVKRL
                     VVQPEDLTKNPPIQEELEPDDDFRQRVQLKLESISGAGPRKAYTYHALSSHGRIADVS
                     VISPKPAYVTVTVTSRVGTGQADDEIINAVTAALNDEDVRPIADRVTVQGAEIVEYTI
                     EAVLKIYKGPEKEPILKAAQANLESYIQHTKRIGRDVTHSGIYAALHVAGVQNVQIKA
                     PKNDLVLTPYQAGYCTGTVLSIEASDEYS"
     gene            15119..15658
                     /locus_tag="PWY43_001208"
     CDS             15119..15658
                     /locus_tag="PWY43_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDL2136092.1"
                     /translation="MNTVKPLLPSGSSLLEKRAAECLQEAVTNRIDFENLINPHKCPV
                     KLLPYLAWALSVDYWEEYWSEQQKRQAIIDSYHNHQRKGTVGAVRRIVESLGYDFEIR
                     EWFKEKRERQAGTFRIFVELKDKGISEKIYQELERLLEDAKPVSRHITELAVSATSRG
                     KANIFSAAHSGEIILIKPR"
     gene            15661..>16682
                     /locus_tag="PWY43_001209"
     CDS             15661..>16682
                     /locus_tag="PWY43_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2136093.1"
                     /translation="MTTQFYSLITKYGESVIAQAVANNTPVPLKTMAIGDGNGTPTTP
                     SESQTTLINEVYRAEITDLLQDSETPNQVIAELLVPETVGGFTVREVGIYDDQNKLVA
                     VANCPENYKPVLEQGSGKVQYYRIVLRVSSSDSVTLSLNNNIVYATRLEFNRFVNDLS
                     KPDGFKLVGECESITQLRTIEPTEDQQRILVKSYHARKNLGGGVFYADFADTTTADNA
                     GTVIVTAGGKRWKRVYSTLNIFDFGVSLDSVNNDAIKRLQAWQEPVLGLGNVIPASSR
                     IVPASHISQVAYRFNGIDYLSEDYYKADLSQITRTGYYTAWTQDKAFVHNGVIYAPFM
                     LAFRHG"
BASE COUNT         5340 a         3436 c         3709 g         4197 t
ORIGIN      
        1 tcattattta tcaataagtt aaaatctata atgtgcagta tatgtatatg caattaaaga
       61 ataacgattt ttcctgacaa gcaatatttt tgcgttgagg tgcgtaaaat tgcgtaaaaa
      121 gatcgagttt ttaagatcgc tttgcgatta tatggggctt gtagggatcg cctttttgca
      181 tttgcgtata aatcgacaaa ttaagtgtgc gggcgaggtg agggatagac cgcgcggggt
      241 tggaggtggt gaagtcgttg aaaaataaag gaaaaattat tttttaagtg tattttattt
      301 gttgttttga tataataaat tgccgaaata tataatctaa tcaggcaaaa cttttgcttt
      361 atatattaaa aaagccccaa tctaggagct gtattgtcag tttgtaaaga tctgttttat
      421 tgtgtccgct gtaacaattt agacggttta aatttgataa cctcttgacc taaccactga
      481 ttaaactctt tcaatcgctc ttgcaatggc tcaatctcgg tgataaaaaa cacctcggca
      541 gcatcagcca cgttgccaaa accgccggtg ttgtttggga taatacccat cagttgtggt
      601 ggcacacggt gagctgctaa cacatcatca cggcttacgt ttttaatatt aaaaaattcg
      661 tcttttgccg atacatcagc aatcggaatt atttgaatgc cgtctttgtt accacctgga
      721 gaatgtacaa atagattttt gaaattcccc accccttttg atttccgcag ttgctctctg
      781 atattatcca catctccctg ttttgcggag ttttccgtca tataaaacac aaagccggcg
      841 tgagctccat ttaaaaaata cttacggcga aacagagtag cactctcatt taaccaagcg
      901 gattgcaacg ccgaaagata ttgtggcaag ccgtaaatat cttgattaat atcaggctca
      961 agcaagtgat agattgagcc tttggcaaat tcgtgttctt ggtgatcgaa tcgttgtggc
     1021 acatagtaaa attgaccagc ctctacaccc tttcgcacat attttgccaa aggtgattcg
     1081 attcgctgta cctgcccaag gcgatttcgc accacctcaa aataggcgtt gccaaatact
     1141 aaataatctt taacaaacga tgacaaatca cggcgtgata aataacggct atcaacctcg
     1201 caagtggaaa gcaaaatatt tgctttggtg atgatcgcac tttcgtggtg tgttgatgac
     1261 cttaacgatt tcgccaagcc atcaaagctc attggtgggt tataccattt ctcatacatc
     1321 gccgaacact ccaaataatt gagaatatcg gcacggtcta acaccggaat cgggtcacca
     1381 aaactgaaaa tttcggtttg tgcttgcggt tgtaagcggt cattttgggt taaatttttg
     1441 cgcaattgtt ttttcatttt ttgtcctaat catctaaaaa atctactgtg ccggtcgttt
     1501 cttccagttt cccatcaaac ggctcattaa taaatacctg caagcaagcc cacgcaaggt
     1561 cggcgtggct tgcctcttcc gaacggtctg ccacataagt cacttgccgt ccgctgttgg
     1621 tgatctgttt tttaattgcc ataaaacttg caccaacctc aacagcgtgc attgagtcaa
     1681 attcaaatcg tcctttgctg ataatatcca agccttttaa taccattttg gatttcagct
     1741 ctacgttgta agtaagagcc accgcatcag gccgttcttt tttcacgatt tcatacacgc
     1801 cgacaccaag ccctgtggta tcaatcgcca gtctggtcac gttatatttt gcacaaatag
     1861 cgataatttc tgccgcttgt tcggcaaaat cagccccttt aaaagtgcga tattccaaca
     1921 aacggaactt gcccccatcg actttcggtg gagcaatcac caccaatgcc gagcggtcgc
     1981 cggtgtaaga aggatcatag cctacccaca cttctttatt gccaaacggt cgcatatagc
     2041 catcggtgaa aacgtagtct ttccaaattt ccattgaatc gaccaagcaa cgctgaatca
     2101 ttgtaaattt aaagacggat tggttatcat caataaactc acacataaag agctgctcga
     2161 actcatcagg cgagttttcc agtttgagtt gctcaatatc aaacaggtta cagccacctg
     2221 cctcggcatc gtagatattc acgatttgtc gccattggcc gtctccgcat tttttgccgt
     2281 ttttcaaatt agcgtgcgaa atatcaattt ccacttgctc cgctttcggt cgtttgcggt
     2341 taaacagctt gcccgaccaa agcagatagg cggaatgggt aatgctcgaa ggggtagaaa
     2401 aataagtaat ccgatattgt ttttggcttg ccatacccgc agcaactttg cggatttctt
     2461 caaagcggtt tacccaaaag atttcatcaa aatagagatt gccgtggtaa gactgtgccg
     2521 ttttcgagtt tgtacccaag aaaattagtt gagactcgtt cggcaaggta atggtttcgc
     2581 cttttagctc cacatcggca acccgtttgg cgtatgccac aatataggct ctaaattgca
     2641 atgcctgttt tttcgaggcg gaaataaaaa tctgatttct accggttact aacgcatcaa
     2701 ctaacgcctc gtgagcaaaa taataagtcg ctccaatttg acgactttta agaatattgc
     2761 ggattcgatg agtcagccca gcttcgtgcc attttttctg ataatggaac attccgtcta
     2821 aaaagccatt aatcagtaat tccgcttgct cttcagaaat agcgttttgt tcaggctttt
     2881 tgcggtcgcc tttatttcgg cttttgatgt tcgggtttaa atccgcctca ttgcctccgc
     2941 cgttagcgta tttcttcact cgagccacac gctcaagctg ccgaccaagc aaatcaatct
     3001 ctttatagtc tgaacctgtt tttacctctt tcataattaa aaggtttaaa cgagcctcaa
     3061 gcgttgcctc aactcgtcca acggggctga tttcgtccca tttctctcgc tttttccaac
     3121 ttgcaatggt agagacggga atatttagct gacgagaaat ttcggtcacg gtgtaacctg
     3181 cccaatactt tgattgagcc tctcgtttat cgtgtagagg gttggcagcc aacacctcgt
     3241 attcagtgat tgtattttca ttttccatag gtgggattat caaaaatcca ccttgtttaa
     3301 gcatttgctt aaggctgtga aacccgattt aacacgtacc acacattgaa accgccggca
     3361 caaaacaaga tcataaatcc gatttttacc cctccccttt agagcaagga aaaacagatg
     3421 acaacaaaat ccaaatggtt tgtggtagca acggaaggag caactacaga tggacgcgaa
     3481 attagtcgtg aatggctcac gcagattgaa gaaaattacg acccgaaaaa taactatggt
     3541 gctcgtatta accttgagca tttccgctgg cgttggtttg agaaagatga cccgcattct
     3601 tacagctatg gcgatgtgtt agcggttaaa acagaagaac gtgaagacgg caaattacag
     3661 ttactggcag aaatcgcccc aaccgaagcc ttagtgaagt tagtcaaaga caagcagaaa
     3721 gtttatacct ctgtggaaat tgaccttaat tttgcggaca caggcgaggc gtatttagtt
     3781 ggtttagcgg tgactgatac accggcaagc ctcggcacgg aataccttac attttgtgcc
     3841 ggtgcaaccc ataacccact agcagaccgc aagcaaaagc cggaaaacct tgtgagtgag
     3901 gcagtagagg cgaatttaga atttactgct gaacctgaaa aaggtgcgtg gctggagaaa
     3961 tttaaggcga tgtttaccaa agccaaaacc gacaatgacg gcaaatttgc cgaacacgaa
     4021 aaggcaatgg aattgttagc ggagcaattt agcaaagttt cgacagaaaa cgaccgcttg
     4081 caaaccgagc taagcacatt gcaaaccgaa tttaccgaat tgcaaaaaca agcgggcgaa
     4141 tttgccgaaa aatttgcaaa actggaacaa gaaccgtctg ccaattacac cccacgccca
     4201 aaagccactg gtgcgaaagc aggtgatgta caaaccgatt tttaattgag gatcacgaat
     4261 gaaagattta acccttgaaa aatataacgc ctaccttgca cgccaagctg aattaaacaa
     4321 cttgccgttt aatgcattgg caaccggcat taaattcaca gtgcaaccat cggtacagca
     4381 aaagctgtat gaaaaggtgc gtgaaagctc tgatttctta aaatctatca gctttgtgtt
     4441 tgttgatgag caaaccggtg aaaccttagg tttagattca gctcacaccg tggccagcac
     4501 caccgatacc agcggtgacg gtgaacgcaa aaccacctct attgcgaagt tggtaaaaca
     4561 aacctaccac tgccaacaaa tcaattttga cacccatatc aattataaac agttggatat
     4621 gtgggcgaaa ttccctgatt ttcagcaaaa agtggcgaat gttgcggcaa aacaacgcaa
     4681 gcgtgatttg attatgatcg gctttaacgg cacaagtcgt gcggcaacca gtgatcgcaa
     4741 cagcaacccg ttattgcaag atgtggcaaa aggctggttg cagaaaatgc gtgaagatgc
     4801 caaagagcgt gtaatgaatg gcgaaagcac agacaaccaa gttttagtcg gcaaaggtca
     4861 agagtatgcc aatcttgacg cactcgtaat ggacgccacc gaagaactta ttgatgaatg
     4921 gcatcgtgat gacaccgatt tagtggtgat taccggtcgc aagttgcttg cggataaata
     4981 tttcccgatt gtgaatcagc aaaacgcacc aaccgaacaa cttgccgctg atattgtgat
     5041 ctcacaaaaa cgcattgggg gcttaaaagc ggtgcgtgtg ccgttcttcc cggcgaatgc
     5101 gattttaatc actaagctgg aaaatttagc catctatgtg caagagggga caacccgcaa
     5161 gcatattgag aacgtgccga aaaaagaccg tattgaaacc tacgaatcag aaaatatcga
     5221 ctacgttgtc gaagattatg gctgtgcggc attaatcgaa aacattacac tcaaagataa
     5281 agagtaaggt ggaactatgc gaatgagtcc ggctcgggct cattttttgc ggaaaaccgc
     5341 agaaagtgag tctgcaaccc caaaccgaga aaaccttgaa ggcttacaag gctatgactt
     5401 agttttagcc caacttaaag cccatcaacg ccaattaaag caaacccagt cgattgagcg
     5461 aaagattgag tttaaacgca acaattttga gcaataccga ccttgggtgg aaggctccct
     5521 tgccaaaggc tccggtgtgc aagacacggt tatcaccaca atgctgattt ggtcgattga
     5581 tattggcgat tacccaaccg cccttgcgat tgctcaatac gttttaatgc acgatttggc
     5641 aatgccggag cagtttgaac gcacaccggc aaccgccttg gttgaagagc ttgcagaggc
     5701 ggcaaagaaa gcccgagacc aaaaacagcc gtttgaggca gccgtgctga aacaggcaaa
     5761 cgacctcacc gctgaatttg atatgcccga cccagtgcga gccaaattac tgcgtgagct
     5821 aggcgagcta atccaagagg cagagccaca aaccgccctt gaacactatc aaagagcgat
     5881 agccctcgac cctcaatgcg gtgcaaaagg cttgcgagac aagttagaaa agcaacttgc
     5941 caaacaagcc gaagagccga aaccagcaga agaacagaat gagtaggcga aagccgaaat
     6001 agagcgtatc cacgccagcc aaggggcgga ttttaaaggg tgatttattc attttgcacc
     6061 cttttaaaat ccccacccct taccttacgg aaaccttatg gaacaacgca atcacgttat
     6121 tagtatcccg aaagtgaatg gctatgaatc cagcaaaaat gcgatcaagc ccaccgcaga
     6181 atctgatgaa actatcacca ataatggctt ttttccgaac attgagctac tggaggtgcg
     6241 aaatgcaatg cggattgatg gcacggtcat taatgagcgg ctaaaacaag cggtgattga
     6301 ggcaatggca acagtcaatg ccgacttaaa agcctatcgc ctcaatgcag agcaagcaca
     6361 aaaagcgaac ttgcaagcct gtgatgatga gcaaatcaac ggcgaaagcg tgctggttta
     6421 caaatataaa cgagcggtct attgccttgc agtagcgaac ctttacgaac gataccgcag
     6481 ttatgacagc accaaagacg gccacgacaa agccgaagag ctagaaagta ccgctggtga
     6541 tttaaagcgg gattaccact ttgccgtgcg ggatattttg ggcgaaaacc gaatgattag
     6601 tgaattaata tgaacacctt gattgcacgc caagacgaca ccttagatga gctgatcttt
     6661 cggcactacg ggcaaacggc aggcttagtc gagcaagcct tagaatataa ccccgaactg
     6721 gctcatttgc cacgtttgcc gatcggcact gtagttacaa tgccggatat tgagagccgt
     6781 tttgcaacgg tggcgaaatc cagcgtgcaa ctttgggatt aaaaaatatg cacaaaactg
     6841 aacaagcctc ctattttggg gcaatttttg gcttttttgg tagcctcacc ctatctgatt
     6901 ggggggtggt agtgagtatt ctggtagcta ttttaacctt attacttaat tgggcatacc
     6961 gagccaaaga atacaaactg aaagagcgag aatttgagct aaagctacaa aaccaaggga
     7021 aaaagaatga gtaaaagtgt aaaaattggc gtatttgtct gctccgttgc agctattatc
     7081 gggacggtaa agcaatatta cagctccgaa atccgcacca gcgaaacagg cttagccatt
     7141 atcggcaatg cggagggttg cagacgtgat ccgtataaat gccctgccga tgtaatcacg
     7201 gttggcattg gctcaaccga ggcaagcggt gagaaaatta atgtcaatca caaatacacc
     7261 gataaagaga ttgccgagcg ttggataaaa gatcttaaaa tagctgaaag gtgtatcaat
     7321 cgttatttta atggcgacaa aatgaacgaa aatcagtttt cggcgatggt atcagcagca
     7381 tttaatatgg gatgttacaa ccttagattt tatccaaatg aaaatggtaa atacattcag
     7441 acaacaatcc ataaatatgc tatggctaag gattttaaag cgatgtgtaa taggatacct
     7501 gattttaacc gctcgggagg taaagtgcta agaggtttga aaatccgccg agaaaaagaa
     7561 aaagccctgt gtctcgggca ggaggttaaa tgattgtcaa actctgccaa atgaccaata
     7621 agcatttcag cccgctaatg attgtgctgg tgctagttgg cgtattagcc aatttcgtgc
     7681 agtttcgcca aaacgaaagc ctgaaagcag acttacaggc acgcaccacg caaaaccaac
     7741 tgattcaagc cgataaccgc aatttagcca atcagcttga aaacagccaa atacagcttg
     7801 aggcgtatca aaaacaggtg gatgacctca atcagaaagt gttagccaaa atgaaacaag
     7861 cggagcaacg aagtaatgaa atccttaacg aactggaaaa gcataaaaag tgggctgatt
     7921 ctgccgtgcc tcctagcgtt ggcaagctgc tcaaccaacg aaagcgtgcg gtatcacacc
     7981 ctcaaatcga acccgataat ctgtccgaaa aggctggagt gccaaacacc ggcaaccgag
     8041 attaacacca acggtgatct tgtcaaagca ctagacaaaa gactcaacac cattgaggta
     8101 tgccaagtca tcatcgaatc gttcgagcat tgcatcgagc aatacaacaa aatcaaccaa
     8161 ggcgagtaat cgcctttttt aataaggacg actatgtcag accaaatcga ccgagccaac
     8221 gaattagccg aaaaagccag agaggcagca cttgcaaaaa ttttgcaaaa tcagaccgct
     8281 tgcaccagcc tctttgagtg tgaggactgt ggcgagccaa tccccgaaaa acgccgagaa
     8341 atggttattg gctgcacccg ttgcattgag tgccaaacca tttacgagca taagcaaaaa
     8401 ggctaccgca gatgattaaa cctgatcgct tgcgagcctt gctcacgcaa accattgata
     8461 tattccaaac caactccgaa aatctgattt tgcaatatga caaaggcaaa attaaaagca
     8521 aagggagcca aagccattcg tttgaatacc actacgattt agagctaatc gtggtggatt
     8581 ttccgtatca ccccgacgtg ttgtttgtgc cggtgctaaa ttttgtgcga aatgaacaat
     8641 gggagctact gcaaaacccg gaactacaag acaaaatcga atttgaaatc gaccacaaca
     8701 atcacgaaag ctacgatatt tatatccgca ttccgctcac ggaacgggtg atcgtgaaag
     8761 agcaagacgg acacctaatc gcaacccacg ccgatgaacc gaaccttgcc gatgtttcgc
     8821 catttacccg tttaaccgaa tatgaggttt acctcaaaga cgaactgatt tatcagtgga
     8881 cagagccaaa tgagtgaacc gattgaacaa gtaaaaaccg cctttgacca cttgctaaat
     8941 aatatcagca aacctcgcag acggttgatg tatcaacaaa tcgggcgaga gcttgcccga
     9001 agccaacgca gacggattaa agcacaacaa aacccagacg gctcggcata cgagccacgc
     9061 aaaaagccga agaaaggggt aaaaagcaaa attaaatcgg gcaagatgtt tgacaaaatt
     9121 acccagccac gttttatgcg gctacgcctt gaaagcgaag gtgtcagctt aggttatgag
     9181 ggtggtgatg cggtcatcgc ccgaatccac caacaaggct taatcggacg agtgcggaaa
     9241 gattgggatt taaaagtgaa atatgccagc cgtgagttgc taggttttac tgatgacgat
     9301 ctgcagatga ttgaggatta tatgattaat attttggctg gtagctaaat gatgaataga
     9361 aacttttgca aaaaagcggt caattttgac cgcttgagtt attgattaac gctttgtcta
     9421 tctgaatgat accaacaaag aaaccctaaa atagctgaaa aaatcaatgc tatcaaaata
     9481 aaagtattga gtggtaacaa tacataaagt gagatcagaa taacgataat agaaagccca
     9541 accatatttg ccaataatga aagcaggctt aaaaatactg ttgtaactac accaaaaatg
     9601 gcagcaaagg ttgtaaaacc tattgccaat aatgcaccga aaccaattaa cccaagtaag
     9661 ataaataaga tttccataga ttttccctta gccatactac aattaaggtt ataatatgag
     9721 caataattta aaaattcaag tagttctttc tgcgttagat aaattaaccg caccatttaa
     9781 gagtgtggta aaacagacgg agaaattatc tcaatcttta aaagtacaga gagatgaatt
     9841 aaaagcattg gaaaaaacac aaagtaaaat gcaatcattt aaaaaaatgg acgatagcat
     9901 taagaaatcc aatgaaacta tactcaaaca gacaaagcat ttagataatc tcaaaaataa
     9961 aatcagccaa atgaaaaatg aacgaattga gctgaaagtg aaaatggatg cgaaaaaaag
    10021 ggaatttcaa caaattacga aaggtggttc agcttggagt gcaagagccg catttttaga
    10081 taaagatttt gaaaaaatgc aaagagaata tgaaaaactc ggtgcatcta tttcaagtgt
    10141 cagccgaaaa agaacagaag aaaatcgggc attaaaggat tctcgccatc aacaagctaa
    10201 gcaactttta tattttcgta gtttaagacg tgaattgaga gcaagcggca ttaatattaa
    10261 agattttgct caaagcgaaa ccaacttaac agacaagatt gcaaaagcaa atcaattaat
    10321 cgaccaacaa aaagcaaaat tagaaaaact aaatgcggtt aaagctcgaa atgaaaaata
    10381 ttgggcaact gtcgaaaagg taaaaaatac cagcgaacgt ttgcataatt taggacaacg
    10441 ctcaatgatt agtggtgcgg caatttctgc ccctgttgtt gggctaggga aaggggttgt
    10501 cggaatggct caaacagcgg gtaaatttga acaatttaat gctattttag aggtaaccga
    10561 gggaagtgct gaaaaagcca agcaaagttt tgattgggtt aaacaatttg cagtagatac
    10621 cccctcaaac cttgatgaag caatggaagc ctttgtaaag ttaagagcat acggtcttga
    10681 tcctaccaat ggcttactac aaacattagg tgatacaggt gctgcaatgg gtaagcctgt
    10741 aatgcaagca gttgaggcaa tagcagatgc tttaacgggt gagaatgaac gcttaaaaga
    10801 atttggtatt aaaggaagtg tagttaaagg tacaaatatt attgagtatg cctacactga
    10861 taaattaggc aaacaacaag tcgcaaaagt aaacaaaaat aaccgaaaag aaattgagca
    10921 aaccctaacc cgtattttta acgaaaaata tgctggagca atggaaaaac aagctaaaac
    10981 gctagttggt atttgggcaa aaattgaaga ttattggaca aatttccaaa tgcaagtgat
    11041 ggaatcggga gcatttgact ggattaaaaa taaattgcaa tgggtgttag acagtttaga
    11101 taaaatggct gaaaatggcg agttgcaaaa atgggctgaa gatgtcggag ccgttattca
    11161 agaaacagta caaggcttat gggcttttgg tgaaaaaatt attgaagtgg ttaaatggtt
    11221 agcgagcttg gcacgggaga ataaagggct aattgcaaca ttcgttaaat ggtctgctat
    11281 tcttggctct agtttaacgg tgctaggagc ttttgcagtg gtggcaagtt ttgcattata
    11341 tcctgtagca agattggctc ttggtatcgg caaatttaca ggtgcaagca ccttattaaa
    11401 taaggcatta tttgatagta atgataaatt ccgattattc aataaatcac tttattcatc
    11461 aggcactacg gctgcttttg ttaaatcaaa atttaatcag ttagcaactt taccaaatgt
    11521 gctgggaagt agttttaata aattaggcgg tggcatcaaa atgctaattc cacagcttaa
    11581 aacaaaagta ttttggctca atatgctaaa aggtatttta ggcaagttgt ggagtgcaat
    11641 ccgttttatt ttctcaccgt tgaaattatt acttggtatt ttctcgccga ttggcttagc
    11701 gattacagct atttcggtag caggggtagc gttatataga aattgggaga aggtcagagc
    11761 cttttttgga ggctttttgc aaggattatc taaaggtctt gcaccagtat tagagagatt
    11821 taaaccttta ggaaaactat ttgaaactat atcaaactgg gttaaatcta cgtttaagtg
    11881 gttatccgat ttattaaccc cagcggataa aacatcggaa agtttagata aagcgagttc
    11941 tgcaggagaa aaattcgggc ttgcattggc tggtgcaata gacttggtaa catcacctgt
    12001 tcagtggcta gtcgatcagt ttaagtggat ttctgaaaat atgccgagtt gggacactat
    12061 tggtgagggt gtaaaaaagg cagctactgc aattgttccc accgagcacg ctaagcagat
    12121 tagtcaaacg gcagaaatgt caaataatat gtatgacccg aattatactc caccacaact
    12181 tgagcaaaaa tggattggtg gcttggttgg taacggtaaa ggaagaggct ttgccacagg
    12241 cggctacacc ggcaacggtg gcaaatacga gcccgccggt attgtgcata agggcgagta
    12301 tgtaatgacc aaagaggcaa ctgcacgctt gggtgtggca aatcttaacc ggctaaacta
    12361 tggcaaagtt gctgggctaa ctgcacttgc gtcaagtgtt gcgtttgctc aaccaatgcc
    12421 tgcggtcaaa atcgacagca gaccaccgct gacggcaagc caaccaagcc caacagttgc
    12481 accggtgtcg caaaatatcc atatcaccat caacgccacc ggtgggcaag acccacaagc
    12541 aatcgcccga ctggttgcaa tggagttgga aaaacaacaa cgccaagcac aagcaagagc
    12601 aagatcgtct ttgagagaca ggggctaatt tgacaaagta gaatgaaaag cggacaatgc
    12661 tctaaattgt ccgcttttta taggagcaaa tgatgtttaa agaaaaagga tatgatgaat
    12721 ttgtgaatga atgtgtgcga aaaggtagag aagaacacaa agctggttta ggatttacgc
    12781 tagagcaatc tcgcttacat atcaaaaaaa ccattgagca aaaatcacaa gagctaaacg
    12841 aatttcagcg tgaagtggtt tatggctaaa attaccatta aaccacaagc attgagtcaa
    12901 tttgacgaga ttgtaaaaag tgttatcgaa tttaccggtt ttgaaacgag tggtattcga
    12961 ttacaaagtg atatttttga caaaatagag caaattgcat ttatgccact ctctggaaga
    13021 gcaagagaag atgggacaag agaaactttc gtaagacgtt atcggattgt gtacgattac
    13081 gataggcagg cagatgaagt gatcatctta gctatcattc acagtagccg actttaccca
    13141 cgcctataat cagcaagcga cctaatcggt cgctttttct ttgcctgtta aaccccattt
    13201 cacaccttgc atttataccc aaagcctttc gccttttgca aaatatcctc attttttaac
    13261 cgcttgtaat tgtaaaaatg agtgagccaa accacgccga acaaatccgc cgtttagaca
    13321 atattatccg ccttggcact attgccgagg tggatttaac cacagccacc gcacgggtca
    13381 attcgggcgg tattacgacc gatttcttac cgtggctgac ttttcgtgcc ggcacatcgc
    13441 aaacgtggtc agctccaaca gttggcgagc aatgcgtggt gttaagcgtg agtggtgaat
    13501 ttaccacagg cgtgatctta tttgggattt acacccaaaa cgccccgagc caatccgccg
    13561 atgagcatct attccgcttt tcggacggtg cagaaatcag ctacaacacc gcaagcggtc
    13621 aattaaccgt aaaaaattgc aaagtggtga ttgtgcaggc gacagaatct atcacgctcg
    13681 acacgcccac ggttaaggcc acaaaaaatg tcgtgattgg gggcaacctc tccgtgaaag
    13741 gcacaacctc atcgcaaggt gcaatcagca cacagggggc ggtaacagca aaaggcgatg
    13801 tatcaggggc gggaatcagt ttaggcagcc accatcacat tgagcaaggt gacggcagac
    13861 caactagcgg agcaaaacca taatgaaccg agaaacaggc gaacagcttg aagacgaagt
    13921 agagcatatc aagcagtcta tcaaagatat tttgctgacg gcaaaaggca gtcgggtaat
    13981 gcggcgaacc tatggcagta atctctacaa gctaactgat aaaccgattg ccgcaagtct
    14041 gattatgcaa ctctccgctg cgtgcgtgat ggcgttgcag caatgggagc cacgtatagc
    14101 gatcacccga tttaaagtgg aatttgccga agataaccac aataagctga tttgcacctt
    14161 agattttacc cttaagcacc gaaaaatcag catcaaagga atgcgtttta aactatgagc
    14221 gaaattgtcg atttatccaa actccccgcc ccaaaagtgc tggaagaatt ggactatgaa
    14281 accttattag cagagcggaa agccaagttt ttatcgcttt accccgaaag cgaaagatcg
    14341 gtaatggctg cacggttagc gttggagtct gagcctatca caaagttact agaggagaat
    14401 tgctatctgc aattaatgga acgccaacgc attaacagtg cagcacaggc aacaatgtta
    14461 gcctatgcca ccggtacaga tttagatgtg attgggggga attacaatgt taaaagatta
    14521 gtggtacagc ctgaagattt aactaaaaac cccccgatac aggaagagct agagccagac
    14581 gatgatttta ggcaacgtgt gcaactcaaa cttgaaagca tttcaggtgc aggccctaga
    14641 aaagcttata cctatcacgc attatcatca cacggcagaa ttgccgatgt gtcggttata
    14701 tcacctaagc ctgcttatgt gactgtgaca gttacctcta gggtaggcac gggacaagca
    14761 gatgatgaaa ttattaatgc ggttaccgca gcacttaacg atgaagatgt gagaccgatt
    14821 gcagatagag taacagtaca aggtgcggag atcgtggaat atacgattga ggcagtgctt
    14881 aaaatttata aaggaccgga aaaagagcct atcctgaaag cggctcaagc aaatcttgaa
    14941 tcctatatcc agcatacaaa gcgaattggt agagatgtga ctcattccgg tatttatgct
    15001 gctttgcacg ttgccggtgt gcagaatgta caaatcaaag caccaaaaaa cgatttagtc
    15061 ttaactccat atcaagcagg atattgcacc ggcacagtat taagcattga ggcaagcgat
    15121 gaatacagtt aagccattgt taccatcagg ctcaagccta ctcgaaaaac gagcagcaga
    15181 atgtttacaa gaagcagtaa caaaccgaat tgattttgaa aatttaataa accctcacaa
    15241 atgccccgta aagttgttgc cttatctagc gtgggcattg tcggttgatt actgggagga
    15301 atactggagc gagcaacaaa aacgacaagc tattattgat tcttaccata accaccaacg
    15361 gaaagggacg gtaggagcag taagaagaat tgttgaatct ctcggttatg attttgaaat
    15421 tagagagtgg tttaaagaaa agcgagaaag acaagccgga acttttagaa tttttgttga
    15481 attaaaagat aagggcatta gcgagaaaat ctaccaagaa ttagaacggt tacttgaaga
    15541 tgcgaaacca gtatcacgcc atattactga gttagctgta tcagctacct caagaggaaa
    15601 agcgaatatt tttagtgcgg cacatagtgg cgaaatcatt ttaattaaac cgagatagat
    15661 atgacaacac agttttatag cttaatcaca aaatatggcg agagcgttat tgctcaagct
    15721 gttgcgaata atacaccagt gccattgaaa acaatggcga ttggtgatgg taacggtaca
    15781 ccaactacgc caagtgagag tcaaactaca ctcattaatg aggtttaccg agctgaaatc
    15841 acggatttgt tgcaagatag cgaaacacct aatcaggtaa ttgcagaatt acttgtgccg
    15901 gaaacagtag gcggctttac cgtccgagag gtcggtattt atgatgacca aaacaaactt
    15961 gttgcggttg ccaactgccc tgaaaattat aagccggtgt tagagcaagg tagtggcaaa
    16021 gtgcaatact accgtattgt gctaagagtt agcagtagtg attcggttac gttgtcacta
    16081 aataataata ttgtttacgc cacaaggctt gagtttaatc gatttgtgaa tgaccttagc
    16141 aagccagatg gtttcaagtt agttggtgaa tgtgaatcta ttactcaact acgcaccatc
    16201 gagccaacag aagaccagca acgtattttg gttaaatcct accacgcccg aaagaacctt
    16261 ggtggtgggg tgttttatgc tgattttgcg gataccacaa ccgcagacaa tgctgggacg
    16321 gtgattgtga cggccggtgg caaacgatgg aaacgagttt attcaacact caatatcttt
    16381 gactttggag tcagcttaga cagcgtgaat aacgatgcca tcaagcgctt gcaagcgtgg
    16441 caagagccgg ttttagggtt ggggaatgtg attccggcaa gttcaagaat tgtgccggct
    16501 agccatatct cacaagtagc ttatagattt aacggtattg attacttgtc tgaggattat
    16561 tataaggcgg acttatcaca aattacccga acaggttatt acacagcttg gactcaagat
    16621 aaagcatttg ttcataatgg agtgatttat gcccccttta tgttggcttt ccgccacggc
    16681 ta
//
LOCUS       DANKBV010000046        16518 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.046, whole genome shotgun sequence.
ACCESSION   DANKBV010000046 DANKBV010000000
VERSION     DANKBV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16518
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.046"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..109)
                     /locus_tag="PWY43_001210"
     CDS             complement(<1..109)
                     /locus_tag="PWY43_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2136094.1"
                     /translation="MPNLKIEQGCLTGQKQVPSPHFSQRPDQEDISLLVI"
     gene            169..1011
                     /locus_tag="PWY43_001211"
     CDS             169..1011
                     /locus_tag="PWY43_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HDL2136095.1"
                     /translation="MLSYRHSFHAGNHADVLKHIVQLLILRALKEKEKGFLYLDTHSG
                     VGRYSLLSFESEKTGEYLEGVARLWERDDLPEEVAFYIDELKKLNRGGKLKYYAGSPL
                     LAVQQLREQDRAILTELHPNDFPLLRQEFAKAKNVQTKRENGFQQLKSAQPPKERRGF
                     VLIDPPYELKEDYELVVKAIEEGYKRFATGVYAIWYPVVLRQHTKRIVRGLMDTGIRK
                     ILQIELAVRPDSDQRGMTASGMIVINPPWQLETQMKKILPYLTEVLVPEGTGSWKVEW
                     ITPE"
     gene            1134..1775
                     /gene="pyrE"
                     /locus_tag="PWY43_001212"
     CDS             1134..1775
                     /gene="pyrE"
                     /locus_tag="PWY43_001212"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL2136096.1"
                     /translation="MQQYKHDFIEFALSRNVLKFGEFTLKSGRISPYFFNAGLFNTGR
                     DLAKLGEYYAKAIQASGIEYDVLFGPAYKGIPIATTVAVALANLFDVDKPCCFNRKEA
                     KDHGEGGNLIGSPLTGKILLVDDVITAGTAIRESMEIINANNAQLAGVLIALNRKEKG
                     KGELSAIQEVERDYDCQVFSIVDFDDLVAFIETSPQYAPYLPKMREYREKYGV"
     gene            1949..3026
                     /locus_tag="PWY43_001213"
                     /pseudo
     CDS             1949..3026
                     /locus_tag="PWY43_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     gene            complement(3087..4277)
                     /gene="metC"
                     /locus_tag="PWY43_001214"
     CDS             complement(3087..4277)
                     /gene="metC"
                     /locus_tag="PWY43_001214"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDL2136097.1"
                     /translation="MSNYKNLSTKLVHTGRKKRNTQGAVNPVIQRASSLVFETLEEKK
                     HATQNRYKGALFYGRRGTLTHFSLQEAMCELEGGAGCYLYPCGAAAVTNAILAFVEQG
                     DHILMTGAAYEPTQEFCNVLLKRMGVSTTYYDPMIGEGLKALVQPNTKVLFLESPSSL
                     TMEVPDIPTLVRVAREINPEIVIMIDNTWSAGVLFPALEYGIDISIQAGTKYLVGHSD
                     VMIGTAVSNARCWDKLREGSYLMGQMVDADSAYITARGLRTLGIRLKQHEQSSIAVAN
                     WLAQRPEVKAVYHPALESCPGHEFFKRDFLGSSGLFSFELCEKLSNDKLANFLEHFEL
                     FSMAYSWGGFESLILANQPEEIAKIRPSIERKLTGTLIRIHVGLEAVEDLIADLEQGF
                     NRIR"
     gene            complement(4336..4875)
                     /gene="dsbB"
                     /locus_tag="PWY43_001215"
     CDS             complement(4336..4875)
                     /gene="dsbB"
                     /locus_tag="PWY43_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDL2136098.1"
                     /translation="MLYFFKNLSQSRSAWFLLMLSCLILEGTALYFQHGMGLVPCVMC
                     IYERVALFGILFAGILGFLGNRYAIIRYLAILIWLGSAFKGLILSIKHHDYQVNPSPW
                     NQCEFKVNFPQTLPLDQWLPQVFAPGPVNCSQSQWEMFGFSMAQWLIFTFAVYLIVAV
                     IVLISQFKRSRQRNNFMFR"
     gene            complement(4938..6479)
                     /gene="nhaB"
                     /locus_tag="PWY43_001216"
     CDS             complement(4938..6479)
                     /gene="nhaB"
                     /locus_tag="PWY43_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HDL2136099.1"
                     /translation="MSSIDALFKSFLGKSPEWYKLCILAFLIINPLIYFFINPFVAGW
                     FLVAEFIFTLSMALKCYPLQPGGLLAIEAVVIGMTNPHHIKEEIMANFDVILLLMFMV
                     AGIYFMKQLLLYIFTKLLVAIHSKKILSLSFCLSAAFLSAFLDALTVIAVIISVGTGF
                     YGVYHKVASGNNFEDSTDITNDEKIISNKEILEQFRGFLRSLLMHAGIGSALGGVMTM
                     VGEPQNLIIASQAEWGFIEFLLRVLPVSLPVLICGIATCFLVEKYKLFGYGDRLPSKV
                     WVVLARYSQLKEQKMTKQDRLKMLVQAIAGIWLIIGLALHLADVGIIGLTIIIICTAF
                     CGITDEHTLGKAFQESMPFTALLIVFFSVVAVIIDLKLFEPIIAFVLSAEPHSQLSLF
                     YIFNGLLSMISDNVFVGTVYINEAKTALDAGIIDREQFDLIAVAINTGTNLPSVATPN
                     GQAAFLFLLTSSFAPLIKLSYGRMVYMALPYTIVLSIVGFLGLEFLLPSMTEVMINWG
                     WFITR"
     gene            6636..7361
                     /gene="fadR"
                     /locus_tag="PWY43_001217"
     CDS             6636..7361
                     /gene="fadR"
                     /locus_tag="PWY43_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDL2136100.1"
                     /translation="MGHSDILKAQSPAALAEEYIVKSIWNNHFPAGSDLPAERELAER
                     IGVTRTTLREVLQRLAREGWLSIQHGKPTRVNDIWETSGPNIIGTIIKLDKSYLPVVI
                     ANVVSLRTRMAEAYIPEAIYLAPEESAKLFDGLDELENTAAAYAEFDYKLFRQFTFLA
                     NKPVYALILNSFKSMYHQVAKIFFADENNRQLTLSFYRSLLCTCLAKDSEKAAACMAK
                     NRQSSGEIWQKLSANIPESFGQL"
     gene            7376..8446
                     /locus_tag="PWY43_001218"
     CDS             7376..8446
                     /locus_tag="PWY43_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2136101.1"
                     /translation="MNEIIFSLEPQDNARLQSLCGAFDEHLALIEKSFNLIIARTGFN
                     FTIQSEDDNHYSATLVQNSVKLIKQLYNETAPIKGKIKELDLEDIHLAIQESRMLLQN
                     QWQAGSQGEIAIKTKRGVIKPRGEHQQAYLRNILSHDISFGIGPAGTGKTFLAVAAAV
                     ESLERQEIRRILLTRPAVEAGEKLGFLPGDLGQKIEPYLRPLYDALFEMLGFEKAQKL
                     MERGVIEIAPLAYMRGRTLNDAFIILDESQNTTTEQMKMFLTRIGFNSKAVITGDITQ
                     VDLPRSQKSGLKHAMEVLKEVPELSFNFFDSKDIVRHPVVAKIVQAYDAWEAVDEERK
                     EQRKLEKLALEQEKILAQAEML"
     gene            complement(8569..10575)
                     /gene="tkt"
                     /locus_tag="PWY43_001219"
     CDS             complement(8569..10575)
                     /gene="tkt"
                     /locus_tag="PWY43_001219"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL2136102.1"
                     /translation="MTERKVLANAIRFLSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPTNPKWADRDRFVLSNGHGSMLIYSLLHLTGYDLSIEDLKQFRQLHSKTPGHPE
                     YGYAPGVETTTGPLGQGITNAVGIAIAEKTLAAQFNREGHKIVDHYTYAFLGDGCLME
                     GISHEACSLAGTLGLGKLIAFYDDNNISIDGHVDGWFTDDTAQRFESYGWQVIRNVDG
                     HNPEQIKFAIENAQAETERPTLIICKTIIGYGSPNKSNSHDCHGAPLGDAEIQAAREF
                     LGWEHAPFVIPAEIYTEWDAKAKGTLAEKDWNAKFAAYEAAYPELAAEFKRRYAGELP
                     ANWEAESKAFIEKLQANPATIASRKASQNAIEAYAPILPEFLGGSADLASSNLTLWSG
                     SKPIRAEHNVDGNYINYGVREFGMSAIMNGIALHGGFIPYGATFLMFYEYAHNAVRMA
                     ALMKQRALFVYTHDSIGLGEDGPTHQPVEQTASLRQIPNLETWRPADQVESAVAWKAA
                     VERKDGPTALIFTRQNLAQQQRTAEQLANISRGGYILRECCEKGSCPDLILIATGSEV
                     DLAMKAADVLDAEGVKVRVVSMPSTNVFDKQDEAYRESVLPRSVTKRVAIEAQLADFW
                     YKYVGFDGRIVGMNSFGESAPADQLFKLFGFTVENVVAKAKEIL"
     gene            10938..11150
                     /gene="rpmE"
                     /locus_tag="PWY43_001220"
     CDS             10938..11150
                     /gene="rpmE"
                     /locus_tag="PWY43_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDL2136103.1"
                     /translation="MKQGIHPEYKEITATCSCGNVVKTRSTVGKDLNLDVCGNCHPFY
                     TGKQRVVDTGGRVERFNKRFSIPSSK"
     gene            complement(11205..12260)
                     /locus_tag="PWY43_001221"
     CDS             complement(11205..12260)
                     /locus_tag="PWY43_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL2136104.1"
                     /translation="MKSLVIKLFGTKKEKKTFKFSKIESVLLKPIGDAIGDAVVHIAH
                     LEQIKKALPNVKIAVLVTSRNKLIFSQANVVDMILEDKVSTYLSQRGHWDLYLDFKPT
                     FTTKSIILDSILKPKFIINFGKKQKSIYNLDTVKNYDFTTVFPKQVHFADYLNYAIFA
                     DFLEKNQVNYNLNIPNELSSFANTLWQEKKIRLLLNPQGSMREISTQELSELLSMIDK
                     RYVQHISCLLTNTTNSAEYLSKIKAPFEISLAPNSDILGYCALVNSADIVIAVDGGGV
                     HISCALGKKLLSFYANHPANIARWYPKPKKDVETFMVIADKWTEDNNDTRGFSLVAPA
                     KWLNHQFAKLIEKYTAC"
     gene            12308..13339
                     /locus_tag="PWY43_001222"
     CDS             12308..13339
                     /locus_tag="PWY43_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL2136105.1"
                     /translation="MNIKATLRKARLAIGKAILDSKNTDLILSEIPNKILFLRHDGKI
                     GDYVVSSFVFREIKKQSPQTQIGVVCSQKNRYLFENNPYIDKLYLVKTKSIPDYIRGG
                     KQLAKEQYDVVIDPTVTLRNRDLLFLRTISAKHYVGYQKQKYRLFDLNVEDRKQHFSE
                     IYQDALALIGFENIETQYDVPLNATSNQSIKHYLYENQIRNYIALNLFGAGSARRFSE
                     QKINELLSCLKQHSDKPIVLLTFPEVTAKLCSFTKKYKNIFVYEKTKSVFDSIELIRF
                     ADLLISPDTATVHIASGLSKKIIAFYSSDEQNFIHWHPNNQAETHILRFRKSVNDLDF
                     AAIKPEWLK"
     gene            13341..14231
                     /gene="ilvY"
                     /locus_tag="PWY43_001223"
     CDS             13341..14231
                     /gene="ilvY"
                     /locus_tag="PWY43_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDL2136106.1"
                     /translation="MDFQSLKLFLDIAQSRSFIRSAEKNYMTASTLTRHIQRIEEELG
                     QPLFLRDNRQVRLTEAGERFLAFAQQGWAEWQQLQNQLSPAQGDLEGELKVFCSVTAS
                     YSHLPPILGKFRQKYPKVDIKLSTGDPALALEQVQSLAADISVAGKPEFLPNNIVFHY
                     IDDIQLSIIAPRVASQATQFLQQSPIDWKNIPFILPVNGPVRKRIDRWFKEQKIKDPI
                     IYATVSGHEAIVPMVALGFGVALLPDIVIKHSPMNNQVSYVNLPSPITPFELGICVQQ
                     KRLQEPIINAFWQLLSDNSA"
     gene            complement(14365..>16518)
                     /gene="lon"
                     /locus_tag="PWY43_001224"
     CDS             complement(14365..>16518)
                     /gene="lon"
                     /locus_tag="PWY43_001224"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDL2136107.1"
                     /translation="PDGTVKVLVEGQTRAKIEQIHDDENGFWAAIQPIYSEYDDENEE
                     LKAIAKTTLTEFENYVKNNKKIPAEIIAKLQKITLEDRLADTIASNLIAPVKKKQELL
                     EQPNLIARFEALLIAMATEMDTLETETRIRNRVKQQMEKNQRDYYLNEQIKAIQKELG
                     NGDDVEQSELDKLKEKIDAAKLPTEVKDKLNTEFKKLKAMPQSSSEATVVRGYIDWIL
                     QMPWHKKSAVKKDLAQAQEILDKDHYGLERVKERIVEYLAVQSRLNKLKGPILCLVGP
                     PGVGKTSLGQSIANATGRKYVRMALGGVRDEAEIRGHRRTYIGSMPGSLMMKMAKVGV
                     RNPLFLLDEIDKMAQDMRGDPASALLEVLDPEQNKAFNDHYLEVDYDLSDVMFVATSN
                     SMNIPPALLDRMEVIRLSGYTEDEKMHIARGHLLAKQQENNGLKEGELTIEDSAILSI
                     IRYYTREAGVRSLEREIAKICRKAVKALVLDKKLKSITVSEKNIEEYLGVKRFDYGKM
                     DSQNRIGEVTGLAWTEVGGDLLTIETASVAGKGKFSYTGSLGDVMKESIQAAMVVVRS
                     RAEKLGIAEDFHEKRDIHVHVPDGATPKDGPSAGIAMCTALISSLTGNPVRKEVAMTG
                     EISLRGKVLPIGGLKEKLLAAHRGGITTVIIPKENEKDLEEIPENAKAALDIHAVETI
                     DEVLALALENPPMGIEILSKAEIKVKKSRAKATVQ"
BASE COUNT         4953 a         3269 c         3403 g         4893 t
ORIGIN      
        1 gaataaccaa caaggaaata tcctcttgat caggacgttg gctaaaatgc ggagacggca
       61 cttgtttttg accggttaaa cagccctgtt cgatttttaa atttggcata gtctaaatag
      121 ggtgaatacg ttaaaatatg gctcatttta ctagaaaaag agaagaatat gttaagttat
      181 cgccacagtt tccacgcagg caaccacgct gatgtgctaa aacacattgt ccagcttttg
      241 atcttgcgag cattaaaaga aaaagaaaaa ggttttttat atcttgatac tcactccggt
      301 gtggggcgtt atagtctgtt aagttttgaa tcggaaaaaa caggcgaata tttagagggt
      361 gttgcccgtt tatgggagcg tgatgattta ccggaagaag tggcattcta tattgatgag
      421 ctgaaaaaat taaaccgtgg cggcaagtta aagtattacg caggctcccc attattggcg
      481 gtacagcaac tgcgtgagca agaccgagcc attcttaccg aacttcaccc caatgatttt
      541 ccgctgttac gacaagaatt tgccaaagcg aaaaatgtgc aaaccaagcg agaaaatggc
      601 tttcaacaat taaaatccgc ccagccgcct aaagagcgtc gaggttttgt gttgattgat
      661 ccaccttatg aattaaaaga agattatgaa ttggtggtta aagcgataga agagggctac
      721 aaacggtttg ccactggtgt gtatgccatt tggtatccgg tggtgcttcg ccaacatacc
      781 aaacgtatag tgcgaggttt aatggatacc ggcattcgca aaattctaca aattgagctt
      841 gccgttcgtc ctgatagcga ccaacgtgga atgacggcaa gtggtatgat tgtgatcaac
      901 ccaccgtggc agcttgaaac ccagatgaaa aaaatcctgc catatttaac cgaggtgctt
      961 gtgccggaag gaacaggttc ttggaaggtg gagtggatta cgcctgagta aattaaatac
     1021 ggatagcaca agcgtggacg cttgcgccat cataaattct gatttgcaat attttttaga
     1081 aaaataaccg cttgtcttat caagaagtgg gggaaatata aaaggaaaaa acgatgcaac
     1141 aatacaaaca cgactttatt gaatttgcct taagtcgcaa tgtgctgaaa tttggcgaat
     1201 ttaccttaaa atcaggcaga atcagcccct attttttcaa tgctgggctt tttaacaccg
     1261 ggcgcgattt agccaaatta ggcgaatatt acgccaaagc cattcaagcc agcggcattg
     1321 agtatgatgt gttattcggg ccggcttata aaggcattcc gattgcaacc acggttgccg
     1381 tggcactagc caacctgttc gatgtggata aaccttgttg ctttaaccgc aaagaggcaa
     1441 aagatcacgg tgaaggcggt aatttaatcg gttctccctt aacgggcaag attttgctgg
     1501 tggacgatgt gattaccgca ggcacggcaa ttcgtgagtc gatggaaatt atcaatgcca
     1561 ataatgccca gcttgccggt gtgctgattg cgttaaaccg taaagagaaa ggcaagggcg
     1621 agttgtctgc gattcaagaa gtcgaacgtg attacgattg ccaagttttc tcgattgtgg
     1681 attttgatga tttggtagca tttattgaaa cgtcgccaca atacgctcct tatttgccga
     1741 aaatgcgtga atatcgtgaa aaatacggtg tttaactttt gcaaaaaaac agcaaaaact
     1801 caccgcttgt cactctctaa tctcaccatt atgaatcact ttcatctttt ggtgagattt
     1861 tttcaattaa aaagccgata aattgattgt gagctttagc taattggctt aggataaact
     1921 cctgtaatac aggagttgga gaactaaaat ggcacatcaa aaattacctt ttcacgcaga
     1981 caccgttggc tcatatttgc gttccgatgc ttggaaaaaa gctcacgcag actataaagc
     2041 aggcaacatt tccctcgaac aacgtgatga aattgtagaa gcggaagtga aaaaattagt
     2101 acaagctcaa ttagatgcgg gcattcaagt tgttacagac ggcgaatatc accgctcttg
     2161 gtggcatatt gatttcttgg aaaatctgaa cggcattgaa ggctatgtgc cggaaaaagc
     2221 ctatgcgttt aaaggggttt tataatacac gttgttgcgg taaagtgtct tggaatccga
     2281 atcacccttt tattgagcat ttcaaaaaat tcaatgcgat tgtaggtggt cgtgcggtgg
     2341 tgaaatttac cattccaagt ccaaaccaat taatgtaccc gattcagtgg gatcacggca
     2401 tttacccaaa ccgtgaggaa tttgccaaag atgtgcaaca ggcttataaa gaggcgatta
     2461 aagcgttcta tgatgccggc tgtcgttatt tgcaaattga tgatgtctat tgggggtcgt
     2521 tgtgtaataa cgttggtcaa ccgacatttg aagccgataa agctcaagcg gtggcgaata
     2581 ttcaagccat tcttgccgat aagccggaag atttaaccat taccacccac gtttgccgag
     2641 gcaactataa atcttcttat ttattgacag gggcttatga tccgattgca gacggcttat
     2701 ttgctcaaac caactatgac ggctatttct tagaatatga tgatgagcat tcaggcggtt
     2761 ttgagccgtt aaaacatttt gccaacaaca aagggcgtgt ggtgttgggc ttaattagct
     2821 caaaattccc tgaattggaa gataaagaag cggttaaagc ccgtattcaa gaggcggcaa
     2881 aatatgtgcc gttagagcag ctttgtctca gtccgcaatg tggttttgcc tcaaccgaag
     2941 agggcaatat aatgtcagaa gcacaacagt gggcgaaagt ccgctttgtg gaagaaattg
     3001 ccaaagaagt ttggggcgaa gattaatgtg agaaaatgcc cgcaacaagc gggcattttt
     3061 tgttaatttt ttgcaattca agccatttag cgaatacggt taaaaccttg ttctaaatcg
     3121 gcaattaaat cttccaccgc ttccaagcca acgtgaatgc gaattaatgt acccgttagt
     3181 tttcgctcaa tagacggacg gattttggca atttcttccg gctggttggc aagaatcagc
     3241 gattcaaacc caccccaaga atacgccatt gaaaacagtt caaaatgctc taggaagttg
     3301 gctaatttgt cattcgagag cttttcgcaa agttcaaatg aaaatagacc gcttgaacct
     3361 aaaaaatccc gcttaaaaaa ttcgtgcccc gggcagcttt ccaacgccgg gtgataaact
     3421 gctttcactt cagggcgttg agccagccag ttagctaccg caatgctact ttgttcgtgc
     3481 tgttttaagc gaatgcctaa ggtgcgtagc ccccgagcgg tgatgtaagc ggaatcagca
     3541 tccaccattt gccccattaa ataagagcct tcacgcagtt tatcccaaca acgagcattg
     3601 gaaacggctg tgccgatcat cacatcagaa tgcccgacta aatatttagt gccggcttga
     3661 atggaaatat caatgccata ttctaaagca gggaacagca caccggcaga ccaagtgttg
     3721 tcgatcataa tcacgatttc tgggttgatt tcacgagcaa cacgcacgag agtcggaata
     3781 tcaggcactt ccattgttag cgagctcggc gattctaaaa aaagcacttt ggtgttcggt
     3841 tgcacaaggg ctttcaagcc ttcaccaatc atcgggtcat aataagtagt tgaaaccccc
     3901 atacgtttta acagcacatt acagaattct tgtgtcggtt cgtaagctgc gcctgtcatt
     3961 aaaatgtggt caccttgttc cacaaaagct aaaatcgcat tggtgactgc cgccgcaccg
     4021 caagggtaaa ggtagcaacc tgctccacct tctaattcac acatcgcttc ttgtaatgaa
     4081 aaatgagtga gagtgccacg tctgccataa aataatgctc ctttgtagcg gttttgtgta
     4141 gcgtgttttt tctcttctaa ggtttcaaac actaaagatg aagcacgctg aatcaccggg
     4201 ttaactgctc cttgtgtgtt acgtttttta cgccctgtat gaacaagttt ggtggaaaga
     4261 tttttatagt tagacataac ttttcctaaa gtaaaatgca taggggcaaa tcaacatttg
     4321 cccctttatt aataattaac gaaacataaa gttatttctt tgacggctac gtttaaattg
     4381 gctgattagc acaatgaccg caacaattaa gtaaaccgca aaagtaaaga ttagccattg
     4441 tgccattgaa aagccgaaca tttcccattg gctttggcta caattaaccg gaccgggggc
     4501 aaacacttgt ggtagccatt ggtcaagcgg caaggtttgc gggaaattaa ctttaaattc
     4561 gcattgattc catggtgatg gattgacctg ataatcgtgg tgtttgattg aaagtattag
     4621 ccctttaaat gcgctaccaa gccaaattag aattgccaag tagcgaataa tcgcatatct
     4681 attaccaaga aatcctaaaa taccggcaaa taaaatacca aataaagcaa ctcgctcata
     4741 aatacacatt acacaaggta ctaatcccat cccatgttgg aaataaagcg ctgtgccttc
     4801 aagtataaga cagcttaaca ttagtaaaaa ccatgcactg cgagattgtg atagattttt
     4861 aaaaaaatag agcatagtta ttcctcattt tgtgaactta aggcattcta ccttatttgg
     4921 tatgagttat ctataaacta acgtgtaata aaccatcccc aatttatcat tacttcagtc
     4981 atagaaggta gtaagaattc taaccctaag aaaccgacaa tagatagaac aatggtataa
     5041 ggtaatgcca tatagaccat tctgccgtaa gacagtttaa ttaacggcgc aaaggaggac
     5101 gttaataaga ataagaaagc agcttgtccg tttggggtag caacagaagg aagatttgtg
     5161 ccagtattaa ttgcaactgc aatcaggtcg aactgctcac gatctataat gccggcatct
     5221 agtgcggttt tagcttcatt aatgtaaaca gtaccgacga agacgttatc tgaaatcatc
     5281 gagagcaaac cattgaaaat atagaataat gaaagctgtg aatgtggttc ggcagataac
     5341 acaaaggcaa tgatcggctc aaataatttt aaatcaataa tcacggcaac aaccgagaaa
     5401 aacacaataa gcaatgcagt aaaaggcata gattcttgga atgctttccc taaagtgtgt
     5461 tcatcagtaa tgccacaaaa tgcagtgcaa ataatgatga tggttaagcc aataatccct
     5521 acatcggcaa gatgtagcgc taagccaata attagccaaa ttccagcaat ggcttgcacc
     5581 agcatcttta agcgatcttg tttcgtcatt ttttgttctt ttagttggct atagcgagcg
     5641 agtacgaccc aaactttgct aggaagcctg tctccataac caaataattt atatttttct
     5701 acaagaaagc aggtcgcaat cccacaaatt aacacgggta agctaactgg taatactcta
     5761 agtaagaatt cgataaatcc ccattccgct tgggaggcaa taattaagtt ttgtggttct
     5821 cccaccatgg tcattacgcc acctaaagct gagccaatac cggcgtgcat caataaactt
     5881 cttaaaaaac cacgaaattg ctctaaaatt tctttattag agatgatttt ttcatcattg
     5941 gtaatatcag tagaatcttc aaagttatta cctgatgcga ctttatgata tacgccgtag
     6001 aaacctgttc caacgctaat gattacagca ataaccgtta aagcatcaag aaaggcggat
     6061 aaaaaagcag cgcttaagca gaaagaaagg gaaagaattt ttttcgaatg aatggcgaca
     6121 agcagtttgg tgaaaatata aagcaataat tgcttcataa agtaaatacc ggcaaccata
     6181 aacattaaaa gaagaataac atcaaagttt gccataattt cttctttaat gtggtgagga
     6241 tttgtcatac cgattacaac tgcttcaatc gctaataaac cacctggctg taatggataa
     6301 cattttaatg ccatagagag agtaaaaata aattcagcga ctagaaacca gcctgcaaca
     6361 aaaggattga taaagaaata aatgagagga ttgataatta aaaatgccaa aatgcacaat
     6421 ttataccact cggggctttt tcctaagaaa cttttgaaca gagcatcaat gctactcata
     6481 taaaggacct caaaatttgt aaacagatat tacaattgta atatagttta gattaaagga
     6541 taatagtatt actgaacttt ttagtcgcaa taattgatta gaaataaaga atagttagat
     6601 tttagataag catatttttt gcttgggggg cattaatggg tcacagtgat attttaaaag
     6661 cgcaaagccc ggcagcgtta gcggaagaat atattgtaaa gagtatttgg aataatcatt
     6721 tccctgccgg ctctgatctt cctgccgagc gagagttagc agagcgcatt ggggtaacaa
     6781 gaacaacttt acgtgaagta ctacaacgtc tagcgcgaga aggttggtta agtattcagc
     6841 acggcaagcc aacaagagtg aatgatattt gggaaacttc ggggcctaat attattggca
     6901 ctattattaa gctagataaa agctatcttc cagtggtgat tgccaatgtg gtgtcattac
     6961 gtactcgtat ggcggaggct tatattccag aagcgattta ccttgctcct gaagaaagtg
     7021 ctaaactctt tgatggacta gatgaattag aaaatacagc agctgcttat gctgaatttg
     7081 attataagtt attcagacaa tttacttttt tagccaataa gcctgtgtat gcgttgatcc
     7141 taaatagctt taaatcaatg tatcatcaag tcgcaaaaat tttctttgct gatgaaaata
     7201 atcgtcaatt aacgttaagc ttttaccgtt cattattatg tacttgtctt gcaaaagata
     7261 gtgaaaaagc agccgcttgt atggctaaaa atcgccaatc tagtggtgaa atttggcaaa
     7321 aattatccgc taatattcct gaaagctttg gtcagcttta acagaaggta gaattttgaa
     7381 cgaaattatt tttagcttag aaccgcaaga taatgctcgt ctgcaatcgc tttgtggtgc
     7441 atttgatgag catttagcct taattgaaaa aagttttaat ctcattattg ctcgaacagg
     7501 cttcaacttt acgattcaat cggaagatga caatcactat tctgctacct tggttcaaaa
     7561 tagtgtgaag ctgattaagc aactgtataa tgaaactgcc ccgattaaag gcaaaattaa
     7621 ggaattggac ttagaagata tacatttggc gattcaggaa agccgaatgc tactacaaaa
     7681 ccaatggcaa gccggctcgc aaggcgaaat agcgattaaa accaagcgag gggtgattaa
     7741 acctcgtggc gaacatcaac aagcctatct gcggaatata ttaagccacg acatcagttt
     7801 tggtatcggg cctgctggca caggcaaaac ctttttagcg gtagcggcag cggtggaatc
     7861 gcttgaacgc caagaaatcc gccgtatttt acttacccgt ccggcggtgg aagccggtga
     7921 aaagctcggt tttttacccg gcgatttagg gcaaaaaatt gagccttact tacgcccgtt
     7981 atatgatgcc ctgtttgaaa tgcttggctt tgaaaaagct caaaagttga tggaacgtgg
     8041 tgtgattgaa attgccccac ttgcttatat gcgagggcgt actttaaacg atgcgttcat
     8101 cattttagat gaaagccaaa acacgactac cgagcaaatg aaaatgttct taacccgtat
     8161 tggctttaat tccaaagcgg tgattaccgg cgatattacc caagtggatt tgccccgtag
     8221 ccaaaaatca ggcttaaaac acgcaatgga agtgctaaaa gaagtgccgg aactcagctt
     8281 taatttcttt gacagcaaag atattgtccg ccaccctgtg gttgccaaaa ttgtgcaagc
     8341 ctatgatgcg tgggaagcgg ttgatgaaga gcgtaaagaa cagcgaaaac tggaaaaatt
     8401 agctttagag caagaaaaaa ttcttgctca agctgaaatg ttatagctta caagcggtca
     8461 tatttcgcta attttttgca aaaaatcgct gaaatttcac cgcttatcat agttgttcaa
     8521 caaaaaagcg ggaagttaat tcccgctttt ctttatctta ttgtttcatt ataagatttc
     8581 ttttgcttta gcaaccacgt tttcaacggt aaagccgaag agtttgaata attgatctgc
     8641 tggtgcggat tcaccaaagc tattcatacc tacaattcga ccatcaaagc cgacatattt
     8701 ataccagaag tcagctaatt gagcttcaat cgccacacgt ttagtgactg aacgtggtaa
     8761 taccgattca cgataggctt catcttgttt gtcaaacacg ttggtgcttg gcattgaaac
     8821 tacacgcact ttcacgcctt ctgcatctaa cacatcagcg gctttcattg ctaaatctac
     8881 ttcagaaccg gttgcaatta agattaaatc cgggcaactg cctttttcgc aacattcacg
     8941 caagatatag ccaccacgag agatgttcgc taattgctcc gcagtacgtt gttgttgagc
     9001 cagattttgg cgggtaaaga ttagtgctgt cgggccgtct ttacgttcta ccgccgcttt
     9061 ccaagcaaca gcagattcta cttggtctgc cggacgccag gtttctaagt ttggaatttg
     9121 gcgtaatgaa gcagtttgtt ctaccggttg gtgggtcggg ccgtcttcac ctaaaccgat
     9181 ggagtcgtgt gtataaacaa ataaggcacg ttgcttcatt aaggctgcca tacgcaccgc
     9241 attgtgtgca tattcgtaga acattaagaa ggttgcaccg taaggaatga aaccaccgtg
     9301 tagggcaatc ccgttcataa tcgcagacat accgaattca cgcacgccgt agttgatgta
     9361 gttaccatct acattgtgtt ccgcacgaat cggttttgag ccagaccata aggttaagtt
     9421 agagcttgct aagtcagctg agccgccgag gaattcaggt aagattggcg cgtaagcttc
     9481 gattgcattt tgtgatgctt tacggcttgc aatggttgcc ggatttgctt gtaatttttc
     9541 aataaaagct ttgctttcag cttcccagtt agccggtaat tcgcctgcgt aacggcgttt
     9601 aaattcagcg gctagttcag ggtatgccgc ttcataagca gcaaatttag cgttccaatc
     9661 tttttctgct aatgtacctt ttgcttttgc atcccactct gtataaattt cagctggaat
     9721 cacaaatggg gcatgttccc aacctaaaaa ttcacgagcg gcttggattt cagcatcacc
     9781 caatggcgca ccgtggcaat cgtgagagtt tgatttattt ggtgaaccat agccgataat
     9841 ggttttacaa ataattaaag ttggacgctc ggtttcggct tgtgcatttt caatcgcaaa
     9901 tttgatttgt tctgggttat ggccatcgac attacggata acttgccagc cataagactc
     9961 aaagcgttgt gcggtatcat cagtgaacca accatcaacg tggccgtcga tagagatatt
    10021 gttatcatcg tagaaagcaa tcagtttgcc taagcctaat gtaccggcga gagagcaagc
    10081 ttcgtgtgag ataccttcca ttaaacagcc atcgcctaag aaagcgtaag tgtagtgatc
    10141 gacaatttta tgaccttcac ggttaaattg tgctgcaagg gttttttccg caatcgctat
    10201 accgaccgca ttggtaatac cttgacctaa cgggcctgtg gtggtttcaa cacccggtgc
    10261 ataaccatat tcagggtgac caggtgtttt agaatgtaac tgacggaact gttttaaatc
    10321 ttcgatagag agatcatagc cggttaaatg aagtaagcta tagattaaca ttgaaccgtg
    10381 tccgttagac agaacaaaac ggtcacgatc tgcccattta ggattagttg gattgtgttt
    10441 taaaaaatca cgccataaaa cttctgcaat atcagccata cccattggtg cgcctgggtg
    10501 gcctgagtta gctttttgta cagcatccat acttaaaaaa cgaattgcat tcgctagcac
    10561 tttacgttct gtcatttgtg ttactcctaa tcgagaatgt tatataaaat ggaaaggata
    10621 tcgaattgga ttctacacta ttttgctaga aaaactcaaa aatggaaatg agaaatgtga
    10681 tctgtatcac aaaaaatgat taaattgtga tttgttttga ttggatttga ttttaataga
    10741 cggttttttc ctaatacaat attttcttga taaagcgaat gtttagtagt tgatctaggc
    10801 aacaaattta tctaaaatac gcactctttt tggcgttgag catttgctca acatttttat
    10861 taattacata tggtacctat cagcggtaga gattctaaca agataggcgg cgatatggct
    10921 tcttatgagg ttccccaatg aaacaaggta ttcacccaga atataaagaa attacggcaa
    10981 cttgctcttg cggtaatgta gttaaaactc gctcaacagt gggtaaagat ttaaacttag
    11041 acgtatgtgg caactgccac ccgttctata ctggtaaaca acgtgttgtt gacaccggtg
    11101 gtcgtgttga acgctttaac aaacgtttca gcatcccaag ctcaaaataa tttgattatt
    11161 gcaaaccccg ctaatgcggg gttttgtttt tcttatatat cgaattaaca agcggtgtat
    11221 ttttctatca attttgcaaa ttggtgattt aaccattttg caggagctac cagagaaaaa
    11281 cctcgagtat cattattatc ctcggtccat ttatctgcga tcaccataaa agtttcaaca
    11341 tctttcttag gttttggata ccagcgtgca atattagctg gatgattggc ataaaatgag
    11401 agcaatttct tgcctaatgc acaagaaata tggacaccac ctccatcaac tgcaattaca
    11461 atatcagcag agtttactaa tgcacaataa cctaaaatat cactattagg tgctaggctt
    11521 atttcaaatg gggctttaat tttcgacaaa tattcggcac tattggttgt attggtcagc
    11581 aaacaactaa tatgttgaac atatctttta tcaatcatag ataatagctc tgataattct
    11641 tgagtagaaa tttcacgcat actaccttga ggattcaata gtaatcgaat tttcttctct
    11701 tgccatagcg tatttgcaaa actgcttagt tcattaggaa tgtttaaatt ataattgacc
    11761 tgattttttt ctaaaaaatc agcaaaaata gcatagttga gatagtcagc aaaatgaacc
    11821 tgctttggaa agacagtggt gaaatcgtaa ttttttactg tatctaaatt gtaaatactt
    11881 ttttgttttt taccgaagtt aataataaat ttaggcttta aaatactatc taaaataata
    11941 ctttttgtgg taaatgtcgg tttgaaatct aaataaagat cccaatgtcc ccgctgcgag
    12001 agataagtgg atactttatc ttctaagatc atatctacca cgtttgcttg agagaaaatc
    12061 aatttgttac gagatgtaac taaaaccgct atttttacat ttgggagagc ttttttaatt
    12121 tgctccaaat gagctatatg cacaacagca tcgccaatag catctccgat aggtttgagt
    12181 aatacggatt ctattttgct aaatttgaag gtttttttct ctttttttgt accaaaaagt
    12241 tttataacta aggatttcat ctctataata ggttgattct tttaatgatt ggttatagta
    12301 agtaaaaatg aatattaaag caacgttaag aaaagcaaga ttggcgatag gtaaagccat
    12361 tttagatagt aaaaatactg atctgatatt atcggaaatt cccaataaaa ttctatttct
    12421 tcgtcacgat ggtaaaattg gtgattatgt cgttagttcc tttgtcttcc gtgaaataaa
    12481 aaaacagtct ccccaaacgc aaataggcgt ggtttgctca cagaaaaacc gatatttatt
    12541 tgagaataat ccttatattg ataagctcta tttggttaag acaaaaagta ttcctgatta
    12601 tattcgtggt ggcaaacaac ttgcaaaaga gcagtatgat gttgtaatcg atccgactgt
    12661 tactttacgg aatcgtgatc tcttattttt acgcactata tcagccaaac attatgtagg
    12721 ttatcagaaa caaaaatatc gattatttga tctaaatgta gaggatagaa aacagcattt
    12781 ttcagagatt tatcaagatg cgttagcttt aatcgggttt gaaaatatag aaactcagta
    12841 tgatgtgcca ctaaatgcca caagcaacca atccataaag cattatttat atgaaaacca
    12901 aattagaaat tatattgcgt taaatctttt tggtgcaggt tcggcaagac gttttagtga
    12961 gcagaaaatt aatgaactgc tgagttgctt aaaacagcat agtgataaac cgatagtctt
    13021 acttactttt cctgaagtaa cggcaaaatt gtgttccttt actaagaagt ataaaaatat
    13081 atttgtctat gagaaaacca aaagcgtttt tgattcaata gaactgattc gttttgcgga
    13141 cttattaatt tcacctgata ctgcaaccgt gcatattgcc tcaggcttga gtaaaaagat
    13201 aattgctttt tacagtagcg atgaacagaa ttttatacat tggcatccga ataatcaggc
    13261 tgaaacccat attctccgtt ttagaaaaag cgtgaatgat ttagattttg ctgcaataaa
    13321 accggagtgg ctaaaataat atggacttcc aaagccttaa acttttccta gatatagccc
    13381 aaagccgtag ttttattcgc agtgcggaga agaattatat gacggcttcc acacttaccc
    13441 gccatattca gcgtatagag gaggagctcg ggcagccgtt gtttctgcgt gataatcgac
    13501 aagtgcgttt aactgaagca ggggaaaggt ttttggcgtt tgctcaacaa ggttgggcag
    13561 agtggcagca gttgcaaaac cagttgtcgc ctgctcaagg tgatttagaa ggagagttga
    13621 aagtgttctg ctcggtaacg gcttcttaca gccatttacc gccgattttg ggaaagtttc
    13681 gccaaaaata tccgaaagtg gatattaaac tgagtaccgg tgatccggct ttggcattgg
    13741 aacaggttca gtcattggct gcagatattt cggttgcggg taagccggaa tttctgccga
    13801 ataatattgt gtttcactat attgatgata ttcaactttc gattattgcc cctcgtgtgg
    13861 cgagccaagc cactcaattt ctgcaacagt cgccgataga ttggaaaaat atccccttta
    13921 ttttgccggt gaacggacct gtacgaaaac ggattgaccg ttggtttaaa gagcaaaaaa
    13981 tcaaagaccc gattatttat gctaccgtct ccggacacga agcgattgtg ccgatggttg
    14041 cactaggctt tggggtcgca ctgctaccgg atattgtgat taagcatagc ccaatgaata
    14101 atcaagtttc ttatgtgaat ttaccctcgc cgatcactcc gtttgagctg ggaatttgcg
    14161 ttcagcaaaa acgcttgcaa gagccgatca tcaacgcatt ttggcaatta ttgtctgata
    14221 attcagcata acaagcggtt agattttgct aaatttttgc aaaaaataga gcaaaaccga
    14281 ccgcttgtag ataaaacttt cagataaaaa ataaggtgcg tgattgtgat ctgcgcacct
    14341 tatgttgttt tagattattg cactttattg cactgtggct ttagctcgcg attttttcac
    14401 cttgatctcg gcttttgaca agatttcaat ccccattggc gggttttcta gtgccagagc
    14461 tagtacctcg tcaatggttt ctactgcgtg aatgtccaac gctgcttttg cattttccgg
    14521 aatttcctct aagtcttttt cattttcttt cggaataatc accgtcgtaa taccaccacg
    14581 atgggctgcc agtaatttct cttttaagcc gccgattggc aatactttgc cacgcaagct
    14641 gatttcaccg gtcatcgcta cttctttacg cacagggtta cctgttaggc tggaaattaa
    14701 tgcagtacac attgcaatcc ctgcactcgg accatctttc ggtgttgcac cgtccggcac
    14761 gtgaacgtga atatcccgtt tttcgtggaa gtcttcagca atgcctaatt tttctgcacg
    14821 agagcgtaca accaccattg ccgcttgaat agactctttc attacatcgc ctaatgaacc
    14881 ggtgtaagag aatttgcctt tacctgcaac agaagcggtt tcaatggtta ataaatcacc
    14941 gccaacttct gtccacgcta aaccggtcac ttcgccaatg cggttttggc tatccatttt
    15001 gccgtagtca aaacgtttta cgcctaagta ttcttcgata tttttctcag aaactgtaat
    15061 ggattttaat ttcttatcca atactaaagc tttcacggct ttacggcaga ttttcgcaat
    15121 ttcacgctca aggctacgca cgcctgcttc acgggtgtag tagcggataa tacttaaaat
    15181 tgcactgtct tcaatggtca gctcaccctc ttttagtccg ttattttctt gctgtttagc
    15241 cagtaaatga ccacgagcaa tgtgcatttt ctcatcttcg gtatagcctg aaagacgaat
    15301 cacttccata cggtcgagca atgctggcgg aatgttcatt gagtttgagg ttgccacaaa
    15361 cattacatcg gataaatcgt aatccacctc taaataatgg tcgttaaagg ctttattttg
    15421 ctcaggatct aacacttcaa gcagagcaga agccgggtcg ccacgcatat cttgagccat
    15481 tttgtcgatt tcatctaaca agaacagcgg attacgcacg ccgactttcg ccattttcat
    15541 cattaggcta cccggcattg agccgatgta agtacggcga tgcccacgga tttccgcttc
    15601 atcacgcacg ccacctaacg ccatacgcac atatttacgc cctgtggcat tggcaataga
    15661 ttgccctaat gaggttttac caacccccgg cggaccaact aagcacaaaa tcggaccttt
    15721 taatttattt agacgacttt gcactgctaa gtattctaca atccgctctt tcactcgttc
    15781 taagccgtag tggtctttat ccaaaatctc ttgagcttgg gctaaatctt ttttgaccgc
    15841 tgatttttta tgccacggca tttgtaaaat ccagtcaata taaccacgca caacggtagc
    15901 ttcggaagag ctttgcggca ttgctttgag ctttttgaac tccgtattca gcttgtcttt
    15961 tacctcggtc ggtaattttg cagcgtcaat tttctcttta agcttatcta actcgctttg
    16021 ttcaacgtca tcgccattac ctaactcttt ttgaatcgct ttaatttgct cgttgaggta
    16081 gtaatcacgt tggttttttt ccatttgctg tttaacacga ttacgaatcc gagtttcggt
    16141 ttcaagcgta tccatctcag ttgccatcgc aattaacaag gcttcaaaac gggcaatcag
    16201 atttggttgc tccaataatt cctgtttttt cttcaccgga gcaattaagt tagaggcaat
    16261 ggtatccgcc aagcggtctt ccaacgtgat tttttgcagt ttagcaataa tttccgccgg
    16321 aattttcttg ttatttttaa cgtaattttc aaattcggtt aaagtggttt tagcaatcgc
    16381 ttttaactct tcgttttcgt catcatattc cgaatatatt ggctgaattg ccgcccagaa
    16441 gccgttttca tcatcgtgaa tttgctcgat tttggcacgg gtttgtcctt ctaccagcac
    16501 tttaactgtg ccgtccgg
//
LOCUS       DANKBV010000047        16073 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.047, whole genome shotgun sequence.
ACCESSION   DANKBV010000047 DANKBV010000000
VERSION     DANKBV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16073
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.047"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..108)
                     /locus_tag="PWY43_001225"
     CDS             complement(<1..108)
                     /locus_tag="PWY43_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2136108.1"
                     /translation="MGVLMKKVTLPSPAKLNLFLYITNKRADGYHELQTL"
     gene            complement(96..728)
                     /gene="lolB"
                     /locus_tag="PWY43_001226"
     CDS             complement(96..728)
                     /gene="lolB"
                     /locus_tag="PWY43_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDL2136109.1"
                     /translation="MKKLTKLLSLSALLVLTACNSVLNAPTEVQQPTVQIPHNDPQWQ
                     QHLAKLSQIQGYTATGQFGYISPEERFSSHFNWRYTNPIQFGLELSSNLSTKSLKLER
                     NKRGLTVSDSEGNSRSDRDVDALMQEIITVSFPIDQFAYWVKGLPEKDGNYIVNEKRQ
                     LSQFSYPLNGTLWKASYVEYHEDRVPNLPKLIVLENGTQTLKIRIEKWAY"
     gene            complement(817..2580)
                     /gene="msbA"
                     /locus_tag="PWY43_001227"
     CDS             complement(817..2580)
                     /gene="msbA"
                     /locus_tag="PWY43_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDL2136110.1"
                     /translation="MQEQITQEKDLSTLKTFKRLWPTISPFKAGLITGAVALVLNALV
                     DSSLIYLLKPLLDEGFGQANNDFLKLMAVLVVVFILVRGITNYIASYCLAWVSGKVVM
                     TLRRHIFQHLMYMPVSYFDNNPTGRLLSRVTYDTEMVASSASHSLVTIVREGAYLLSL
                     FVVMIYTSWQLSIVLFVMAPIIGVLISFVSKYFRQLSRNIQSSMGELTVTTEQMLKGH
                     KVVLSFGGQEIEKARFDRVSNDMRRKGMKVVSADSISDGVVQLIASFALSAVLYVATF
                     PEVMSENLTAGSFTVVFSSMMAMLRPLKSLTNVNSQFQRGMAACQTLFEFLDLKTEKD
                     HGSISVVKAEGNVEFKNVSFSYEGKDEKALNNISFTIPKGKTVALVGRSGSGKSTIAN
                     LLTRFYDVTDGEILLDGINIQDYRLKNLREQCSVVSQQVHLFNDTVANNIAYAATDKY
                     TREEIIVAAKAAHAMEFIEKLENGLDTVIGENGASLSGGQRQRLAIARALLRNSPVLV
                     LDEATSALDTESERAIQSALEVLQKDRTVLVIAHRLSTIEKADEILVVDHGNIIERGS
                     HTELLAQGGAYKQLHSLQFGA"
     gene            complement(2664..3350)
                     /locus_tag="PWY43_001228"
     CDS             complement(2664..3350)
                     /locus_tag="PWY43_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL2136111.1"
                     /translation="MSYLYTVLTVIMFIAGLKISQKLKSSFLNPFLIALALLIGVLLI
                     FNIPYQDYYQGNSPLNNLLGVSVVALAYPFYEQLPHIRKKWRQIVVILLFSTIITMIT
                     GLGFSLLFGASQEILAAILPKSVSMPMAIIITNEINGNVAIAAVGVMIAGLVGSILGF
                     TLLRLAKVRNVMAIGLSMGAASHALGTARCLEHSMKAGSYSSIALVACGVLSSIFAPL
                     IFNVIVTLCY"
     gene            complement(3352..3717)
                     /locus_tag="PWY43_001229"
     CDS             complement(3352..3717)
                     /locus_tag="PWY43_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL2136112.1"
                     /translation="MLSTLLRMVASLALLFVMLYLGKLIVYLFPIGIPDSILGMLILF
                     GCLVIGIVKVEWVIPSAKPLTRYITLFFLPICVELIEHLELLRQNLNTFVLATALSTL
                     VSVVAIGVFAQWIFHYKRG"
     gene            complement(3791..5716)
                     /locus_tag="PWY43_001230"
     CDS             complement(3791..5716)
                     /locus_tag="PWY43_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL2136113.1"
                     /translation="MTKYDDKIQHAFSENGLLSQNISGFRPRQAQLEMAQVVAKAVKF
                     ATPVVVEAGTGTGKTFAYLVPALLSGKKTILSTGSKNLQDQLFNRDLPTIQKALKYKG
                     KIALLKGRANYLCLERLDQVTAMGVLGDKSVLTDLKKVSKWQNATKTGDLSECITIAE
                     DSPILPQLVSTTESCLGSDCPHFKDCYVVQARRKAMEADVVVVNHHLFCADMAVKETG
                     FGELIPEAELVIFDEAHQLPDIASQYFGQSLSSRQLFDICKDTNIVYRTELKDLSQLG
                     KAADHLTKVVQDFRLLMGGEGQIRGNLRELFRDKKVVDGLVKVAENIDFLSEVAKKSL
                     GRSEMLDKIFERLAEVKVQLKKLMDTSVTGYCYWYEANGRSFGLHITPLMVADKFGEQ
                     LKNQQIGWVFTSATLEVGGKFDHFCQRLGIENAEQLVLQSPFDYQHQSLLCVPRYLPD
                     TNKSNTLTALGQLLKPVIEANKGRCFLLCTSYYMMRGLADFLREHSSLNVLLQGETSK
                     SRLLEKFVKEDHSVLVATQSFWEGIDVRGDDLSLVIIDKLPFTSPDEPLLKARMEDCK
                     IQGGDPFNDIQIPEAVITLKQGVGRLIRDVTDRGVVIICDSRLVMRNYGQTFLKSLPP
                     SRRTRDLGNVIQFLKQK"
     gene            complement(5899..6120)
                     /locus_tag="PWY43_001231"
     CDS             complement(5899..6120)
                     /locus_tag="PWY43_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136114.1"
                     /translation="MLSIFKTPVEKETLDDWAKISVDVAKVAILAIPVILFGKEPTLI
                     KLTNLALLVLSIYVFLTLGRKLRQLKEVL"
     gene            complement(6207..7004)
                     /locus_tag="PWY43_001232"
     CDS             complement(6207..7004)
                     /locus_tag="PWY43_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2136115.1"
                     /translation="MIELNNLFITFNKGTAIENPVLRGLSLKVEQGEFVSVIGSNGAG
                     KSTLLNAISGDCAVDSGEILIQGNKVNQSTTWQRANQVARVFQDPMAGTCESLTIEEN
                     MALAYQRGRKRGLGFAIKRQMRELFKDKLALLGLGLENRLTDQMGRLSGGQRQAVSLL
                     MASLQPSNVLLLDEHTAALDPKTTDFVLELTNKIVREQKLTTLMVTHSMRQALDYGDR
                     TVMLHQGQVAFDVSGEQRQKMDVPDLLQLFQQSRHEQLSDDGLLLGN"
     gene            complement(7006..7938)
                     /locus_tag="PWY43_001233"
     CDS             complement(7006..7938)
                     /locus_tag="PWY43_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2136116.1"
                     /translation="MDSLIHFLSAFLTNITWIGALELGLIYSLVALGVLISYKILDFP
                     DLTADGSFPLGGAVCVISILNQVDPWLATLYGTLAGAVAGMVTASLHIGFKIEKLLAS
                     ILMMIALYSINLRIMGMPNIAILGETTIYDVMPIESDLQLALVRLVMTLLVVIIVKGL
                     FDLFFATEIGLAVRATGTNSRMAKAQGIAINKMTILGMAISNALIALGGALYVQSNGG
                     VDISIGVGTIVIGLAAVIIGEAIFSVKRIIWLTFAVIIGSILYRFFIALALNNETLNG
                     IGFGPQDLNLITALLVVIVLVVPRIKHKLLGKRG"
     gene            8180..9913
                     /gene="dauA"
                     /locus_tag="PWY43_001234"
     CDS             8180..9913
                     /gene="dauA"
                     /locus_tag="PWY43_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDL2136117.1"
                     /translation="MLNKWFLTKNAFLAVKPFSALKDALNAGYGKQHLIRDIISGLTV
                     GVIAIPLSMALAIASGVPPQHGLYTAIVAGIVIALTGGSRFNISGPTAAFVVILYPVT
                     QQFGLSGLLMATMLSGGILVLMALLRLGRLIEYIPLPVTLGFTCGIGIVIATLQIKDF
                     LGLSIEKMPEHYLGKLEAIFSALPTINWADTLVGVVTLLVLTRWHKLRLPIPGHLPAV
                     FIGTLLSLLLIHFGQNVATIGSAFHYTLSDGTTGYGIPSVLPEFVLPWNLPNAEGNRI
                     DWNLTTIQALLPAAFSMAMLGAIESLLCAVVCDNMTDTKHHSNNELLAQGLGNIASPF
                     LGGITATAAIARSAANVRSGAVSPISSVVHALLVLFALLFLAKALSYLPLSSMAALLL
                     VVAWNMADIPQIIQLTRRSGKNEIAVLLTCITLTVLFDMVIAITVGVLLASLLFIRTI
                     AEMTKSFEMAHPTDLNDVLVYRISGPLFFAAADNLFADLHNKTVHTDHQIKHIVLQCD
                     AVTVLDAGGIHALTRFVQHMLPHQQLHLCNVQFQPMRMLVRSQKTVNEIPQIQFSSDL
                     ESAFDKIRQAN"
     gene            complement(10005..12833)
                     /gene="uvrA"
                     /locus_tag="PWY43_001235"
     CDS             complement(10005..12833)
                     /gene="uvrA"
                     /locus_tag="PWY43_001235"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2136118.1"
                     /translation="MQHIDIRGARTHNLKNINLTLPRDKFIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTISQMVDRVLEEPEGKRMMLLAPVVKERKG
                     EHVKLLENLTASGYLRARIDGEICDLSDPPALELQKKHTIEVVVDRFKVRADLATRLA
                     ESFETALDLSGSTAIIADMDDPNAEEIVFSSSFACPHCGYSLHELEPRLFSFNNPAGA
                     CPTCDGLGVEQFFDAKKVVQNEEVSLAAGAIKGWDRRSFYYFGLLKAVAEHYGFDLET
                     PFGQLPKKYQNIILNGTKEEIEFVYVNDRGDKVKRKHAFEGVLNNMARRYKETESNAV
                     REELAKYINNRPCMDCEGSRLRREARYVFLDKTNLPMVSEKSIGEALTFFEELKLAGQ
                     KEKIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVM
                     YVLDEPSIGLHQRDNERLLNTLVHLRNLGNTVIVVEHDEDAIRAADHIVDIGPGAGVH
                     GGQVIAQGTAAEIMQIEESITGQFLSGKQKIEIPAQRVPYEPTKLLTLSGASGNNLKK
                     VKLEIPVGLFTCITGVSGSGKSTLVNDTLFLLAQNALNRAENSDVAPYESIDGLSHFD
                     KVIDIDQSPIGRTPRSNPATYTGLFTPIRELFAGTQEARARGYNVGRFSFNVRGGRCE
                     ACQGDGVIKVEMHFLPDVYVPCDHCKGKRYNRETLEIRYKGKTIHQVLEMTVEEAREF
                     FAPVPMIARKLQTLMDVGLSYIRLGQSSTTLSGGEAQRVKLATELSKRDTGKTLYILD
                     EPTTGLHFADIKQLLGVLHRLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEI
                     IATGTPEEVAKDKRSHTARFLKEILAKG"
     gene            12988..13503
                     /locus_tag="PWY43_001236"
     CDS             12988..13503
                     /locus_tag="PWY43_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2136119.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GERREVTEWHRIVFYRRQAEVCGQYLRKGSQVYVEGRLKTRKWQDQNGQDRYTTEIQG
                     DVLQMLGSRQGNDMGGQQGGWGNSAPAGNSYNQGNSNYGYNQTQSAAPQSKPAPQPEP
                     AMNNFDDDIPF"
     gene            14195..14506
                     /locus_tag="PWY43_001237"
     CDS             14195..14506
                     /locus_tag="PWY43_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2136120.1"
                     /translation="MVIIMLSKTKSPTLFPKYQNALLQLGENIRLASKRRMFTQTLLA
                     QRTGLSRVTVRKIMNGDPTVAIGYYLMVLGVLGLEQDFTDVGKDDELGRKLQDIELLR
                     K"
     gene            14506..15747
                     /locus_tag="PWY43_001238"
     CDS             14506..15747
                     /locus_tag="PWY43_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDL2136121.1"
                     /translation="MMNKRIYVFADWETFSQPELVGVLSSDVVRGKENFRFSYDNEWL
                     SSPYVQQIDPNLYLYPGEQYSTDTQNFHIFLDSCPDRWGRLLMKRREAALASQENRKP
                     KLLIESDYLLGVHDMNRMGALRFKTELNGPFLDNNTRLAAPPLSSLRELEFAAIQLES
                     SDNVDDPDYLKWLHMLISPGSSLGGARPKASVIDELNQLWIAKFPSRYDDYDIALWEY
                     LAYRLAIQAKIEMATCRIERFNQEHHTFLTQRFDRHCGKRRHFTSALTQLGYYDGDYE
                     ASYLEIAQFLTESGSNTRIDLAQLWRRIVFNIAISNTDDHLRNHGFIYAQEGWILSPA
                     YDLNPVPLSQGLHLNITDSDNRLDYQLAFDVADFFQLSKQQAVQIYDEVLKEVNNWQN
                     VAKEIGIPRQEQEQMKFAFNV"
BASE COUNT         4757 a         3580 c         3090 g         4646 t
ORIGIN      
        1 cagcgtttgt agctcgtgat aaccgtctgc ccgcttattg gtaatgtata aaaacaggtt
       61 taattttgcc gggctgggta aggtgacttt cttcattaat acgcccattt ttcaatgcgg
      121 atcttcaacg tttgagtgcc gttttcaagc acaatcagct tcggtaagtt tggtacacga
      181 tcttcgtgat actccacata gcttgctttc caaagcgtgc cattaagcgg ataactaaat
      241 tgcgacagct ggcgtttttc attcacaata tagttgccat ctttttcagg caagcctttt
      301 acccaatagg caaactgatc gataggaaac gaaacagtga taatctcttg cattaacgca
      361 tcaacatcac ggtcagaacg gctattaccc tcactgtccg aaaccgttaa gcctcgttta
      421 ttacgctcta atttcagtga tttagtcgag agattcgagg aaagctctaa cccaaattgg
      481 attggattag tataacgcca gttgaaatgg gaagaaaaac gctcttcagg cgaaatatag
      541 ccaaattgcc ccgttgcagt atagccttga atttggctca gtttcgctaa atgctgctgc
      601 cattgtggat cgttatgcgg aatttgtact gttggttgtt gcacctctgt tggggcattt
      661 agtaccgaat tacaggcggt taagaccagt aaagcactaa gagataaaag ttttgtcagt
      721 tttttcatag tatccaaata aattattgta gggacgaaat atttttcgcc ctgtttttat
      781 cattaattgg ggagaaacat atttctcccc tacaaattag gctccgaatt gtaaactatg
      841 gagttgttta taagccccac cttgtgctaa caattccgta tggctgccac gctcgataat
      901 atttccgtga tcgactacca aaatttcatc ggctttttca atcgtggata accggtgtgc
      961 tataaccagc accgtacgat ctttctgtag cacttcaagt gcagattgga tcgctctttc
     1021 tgactccgta tctaatgcag aggtggcttc atccagcaca agcacaggag aatttcgcag
     1081 taatgcacga gcgattgcca agcgttggcg ttgcccaccg gaaagacttg ctccgttttc
     1141 accgatcact gtatctaagc cattttccag tttttcgata aattccattg cgtgtgcagc
     1201 ttttgcagcg acaataattt cttctcgagt gtatttatcg gtggcagcat aggctatatt
     1261 gttggcaaca gtgtcgttaa ataaatgcac ctgctgcgaa accactgaac attgttctct
     1321 taaatttttt aagcggtaat cttgaatgtt aatgccatca agcaaaattt caccgtcagt
     1381 cacatcataa aaacgggtaa gcaaattggc aatggttgat ttacccgagc cggaacgccc
     1441 aaccaatgct actgttttgc ctttaggaat ggtgaaagaa atattattta atgctttttc
     1501 atctttgcct tcataactaa agctcacgtt tttaaattcc acattgcctt ctgctttaac
     1561 aacagagatt gagccgtgat ctttttcagt ttttaaatct aaaaattcaa ataatgtttg
     1621 gcaggctgcc attccacgtt ggaattgtga gttcacattg gtgagtgatt ttagagggcg
     1681 taacattgcc atcatcgaag aaaacactac tgtgaatgaa cctgcggtta aattctcact
     1741 catcacttca gggaaggtcg caacgtaaag caccgcagat aacgcaaaag aggcaataag
     1801 ttgcaccaca ccgtcagaaa tactatcagc agaaacgact ttcatacctt tacgccgcat
     1861 atcattactg actcggtcaa aacgagcttt ctcaatttcc tgcccgccaa aggaaagtac
     1921 caccttatgc cctttaagca tttgttctgt cgtgacagta agctccccca ttgaactttg
     1981 gatattgcga cttaattgac gaaaatattt tgaaacgaaa ctgatcagta ccccaatgat
     2041 aggagccatc acaaataaca caatagaaag ctgccaactg gtatagatca tcaccacaaa
     2101 caatgacagc agataagccc cttcacgcac aatagtcact aaagaatgag aagcagaact
     2161 cgccaccatt tcggtatcgt aagtcactcg tgagagcaaa cgcccggtcg ggttgttatc
     2221 aaaatagctt accggcatat acattaaatg ttgaaaaata tggcggcgta atgtcattac
     2281 taccttgcct gacacccaag ctaaacagta actggcaata taattggtaa taccgcgcac
     2341 taaaataaaa actactacaa gcaccgccat caatttcaga aaatcattat tagcttgccc
     2401 aaagccttca tctagcaagg gtttgaggag atagataaga ctagaatcca ctaacgcatt
     2461 taatactaaa gcgacagcac ctgtaatcag acccgcttta aacgggctga tagtcggcca
     2521 taaacgctta aaggttttaa gcgtagaaag atctttttct tgtgttattt gttcttgcat
     2581 aattctctat tttcattgaa aaatcacggt attgtacctt gatttcagcc agttaccaaa
     2641 taaggtttga gatttcactg tttttagtaa cataacgtga cgataacgtt aaatattaaa
     2701 ggtgcaaata tagaagacag cacaccacaa gccactaatg cgatagaact atagctaccg
     2761 gctttcatac tatgctctaa gcatcttgcc gtgcctaatg cgtgggaagc agctcccata
     2821 cttaacccaa tcgccatcac atttcttact tttgccaggc gcagaagcgt aaagcctaaa
     2881 atagatccca ctaagcctgc gatcatcacc ccaaccgctg cgattgcaac atttccatta
     2941 atttcattcg taataataat tgccattggc attgataccg atttaggcaa aatggcggct
     3001 aagatctctt ggcttgcccc aaataataat gaaaacccaa gcccggttat catcgtaatg
     3061 atggtactaa acagcaaaat cacgacaatc tgtcgccatt ttttacggat atggggtaat
     3121 tgttcataaa aagggtaagc aagtgccaca acagaaacgc caagtaagtt attcaagggg
     3181 gaattacctt gataatagtc ttggtaaggg atattaaaaa tcagtaatac accaattagc
     3241 aaagcgagtg caattaagaa agggttcaga aaactgctct tgagtttttg gctgattttc
     3301 aaaccggcaa taaacatgat taccgtcaac acagtgtaaa gatagctcat tttaccctct
     3361 cttgtaatga aaaatccact gtgcaaacac gccaatcgcg accacagaaa ctaaggtgct
     3421 taatgccgtt gctaatacaa aagtgttcag attttggcgt aacagctcaa gatgttcgat
     3481 gagttcgaca caaatcggaa ggaaaaatag tgttatataa cgtgtgagcg gtttcgcgct
     3541 cggtatcacc cattccactt taacaatgcc gataaccaag cagccgaata agataagcat
     3601 acctaaaata ctatccggga tcccgatggg gaatagataa acgattaact tgcctaaata
     3661 aagcatcaca aataacaatg ccaaagaagc aaccatacgc agcaatgtag acaacatata
     3721 actcctcaac aagcggttat ttttatccgt ttttttgcaa aaatctgcag aaatctgacc
     3781 gcttgtaacc ctatttttgt ttcaaaaatt gaataacatt ccccaaatct ctagtgcgtc
     3841 tagacggcgg taaacttttc aagaaagttt gcccataatt tcgcatcacc aaacgggaat
     3901 cacaaataat cactacacct ctatcggtca catcacgaat taaacgccct acaccttgtt
     3961 tgagggtaat caccgcttcg ggaatttgaa tatcgttaaa cggatcaccg ccctgtatct
     4021 tacaatcttc cattcgggct ttgagtaatg gttcgtccgg tgaagtgaac ggcaatttat
     4081 caataatcac caacgataaa tcatcgcctc gcacatcaat cccctcccaa aaactttggg
     4141 tcgccaccaa cacgctgtga tcttctttca caaatttttc caataaacgg cttttgctgg
     4201 tttcgccttg caataacaca ttcagcgaac tgtgttcgcg taaaaagtcg gctaaacctc
     4261 gcatcatata ataggaagta caaagcaaga aacaacgccc tttattcgcc tcaatcaccg
     4321 gttttaataa ttgcccgaga gcagttaaag tattggattt attggtatcc ggcaagtagc
     4381 ggggcacaca gagtaaagat tgatgttggt aatcaaacgg actttgtaac accaactgct
     4441 ccgcattttc gatgcccagc cgttgacaaa aatgatcgaa tttgccccca acttcgagtg
     4501 tggctgaggt aaacacccaa ccaatttgct ggttcttcaa ctgttctcca aatttatctg
     4561 ccaccataag cggcgtaata tgcagcccaa aggatcgtcc attcgcctca taccagtaac
     4621 aatagcctgt cacactagta tccattaatt ttttaagttg aaccttaact tctgctaacc
     4681 gctcgaaaat tttatccaac atttccgacc gccccagcga ttttttcgcc acttcactta
     4741 aaaaatcaat attttcagcc acttttacca agccatcgac cactttttta tcacgaaata
     4801 actctcgcaa attgcctcga atctgccctt ctccgcccat caataatcgg aaatcttgca
     4861 ctactttcgt caagtgatca gccgctttgc ctaattgaga cagatctttg agttcagtgc
     4921 ggtaaacaat attggtatcc ttacaaatat caaataactg gcgggaagaa agcgattgcc
     4981 caaaatattg gctggcaata tccggcagtt ggtgagcttc atcaaagatc accagctcag
     5041 cttcagggat caactcacca aaaccggtct cttttactgc catatcagca cagaataggt
     5101 gatggtttac caccactacg tctgcctcca tcgctttacg gcgggcttgt accacataac
     5161 aatctttaaa atgcgggcag tccgagccta agcaactctc ggtggtgctg actaactgcg
     5221 gtagaatcgg gctatcttcg gcaatggtaa tacattcact taaatcccct gttttagtgg
     5281 cattttgcca tttactgact ttttttaaat cggtaagaac ggatttatcc ccaagtacgc
     5341 ccattgcggt cacttgatct aaacgctcta aacacaaata gttcgcacgc cctttgagta
     5401 acgcaatttt gcctttgtat ttcagggctt tttgaatagt gggaagatct cgattaaaaa
     5461 gttgatcttg caggttttta gaacctgtcg aaagaatagt tttcttgcct gaaagtagtg
     5521 ccggcacaag ataagcaaag gttttgcctg tgccggttcc cgcctctacc acaacaggtg
     5581 tcgcaaattt tactgctttt gcgaccactt gagccatttc aagctgtgct tggcgtggtc
     5641 gaaatccact gatattttgg ctgagcaacc cattttcgct aaatgcgtgc tgaattttat
     5701 cgtcgtattt tgtcatttaa aaacaggcaa gcggtcgaat ttgcaaaata ttttgcaaaa
     5761 acaaccgctt gtgaaaaatt aaatattatt gatttttgga taattgttct ttagaaatcc
     5821 ggttaataat ataaagccaa gcaaacgcaa agacgtttaa aattagcaaa cctatggtta
     5881 aattccagcc tagttccatc acaacacctc ctttagctgc cgtaattttc gcccaagcgt
     5941 caaaaaaaca taaatactca ataccaataa tgctaagtta gtcaatttaa tcaacgttgg
     6001 ttctttgcca aaaagtataa caggaattgc caaaatcgca actttcgcta catcaaccga
     6061 aattttagcc caatcgtcta gcgtttcttt ctctaccggt gttttgaaaa tgcttaacat
     6121 tgaatacctc cttttgggct tattatttaa aatttgacag ccaaaaacaa aaggagaaat
     6181 tcattttttc gatgcagatc gcaaaattaa ttgccgagta ataatccatc gtcactcaac
     6241 tgctcgtggc gactttgttg gaaaagttgt aacagatccg gcacgtccat tttttgccgt
     6301 tgctcgcccg aaacatcaaa cgccacttgc ccttggtgta acatcacggt tctatcgcca
     6361 tagtcaaggg cttgccgcat tgaatgcgtt accattagcg tggttaattt ctgttctctt
     6421 acaattttat tggtaagttc aagcacaaaa tcggtggttt tcggatctag tgccgcggtg
     6481 tgttcatcta acaataagac attagacggc tgaagtgatg ccatcagcaa gctcaccgct
     6541 tggcgttgcc cgcctgataa acgccccatt tgatcagtaa gccgattttc caaacctagc
     6601 cctaacaaag caagtttgtc tttgaataac tctcgcattt ggcgtttgat cgcaaagccc
     6661 aacccacgtt tacgtccacg ttggtaagcc agtgccatat tttcttcaat cgttaaactt
     6721 tcacaagtgc cagccatcgg gtcttggaaa actcgggcga cttgatttgc ccgttgccaa
     6781 gtggtggatt ggtttacctt atttccttga attaaaatct caccggagtc caccgcacaa
     6841 tcaccactga tcgcatttaa taaggtagat ttacccgccc cattgctacc aattaccgac
     6901 acaaattcgc cttgctcaac ttttagggat aagcctctca gcacagggtt ctcaattgct
     6961 gtccctttgt tgaaggtaat aaataagtta ttgagttcaa tcatattaac ctcgcttacc
     7021 gagcaatttg tgtttaattc gaggaaccac taatacaatc accaccaata acgccgtaat
     7081 taaattcaga tcttgcgggc caaagccaat gccgtttaaa gtttcgttat ttaatgctag
     7141 tgcgatgaag aaacgataaa gaattgagcc aataatcacc gcaaaggtaa gccaaataat
     7201 ccgttttacc gaaaaaattg cttccccgat aattaccgcc gccaagccaa tcacgatagt
     7261 tcctacccca atcgaaatat ctacaccacc gttgctttgc acatacaaag caccacctaa
     7321 ggcaattaac gcattagaaa tcgccatccc taaaatcgtc attttattaa tcgcaatacc
     7381 ctgtgctttc gccattcggc tattagtacc ggttgcccgc acagccaaac cgatttcggt
     7441 cgcaaaaaag aggtcaaaca aaccttttac gataataaca accagcaatg tcatcactaa
     7501 tcgtactaag gctaactgta gatcgctttc aatcggcatt acatcgtaaa tcgtggtttc
     7561 gcctaaaata gcaatattcg gcattcccat aatgcgtaga ttaatcgaat agagtgcgat
     7621 catcattaaa atactggcaa gcaatttttc gattttaaag ccgatatgca aacttgcagt
     7681 aaccataccg gctactgctc cggcaagcgt gccgtataaa gttgccagcc aaggatcaac
     7741 ttgattcaaa atgctgatca cacacaccgc ccctcctaaa gggaaactgc cgtctgcggt
     7801 cagatccgga aaatccagaa ttttataaga aattaatacc ccaagggcaa caagggaata
     7861 aattaacccc agctctaacg ccccaatcca agtgatatta gttaaaaagg cactcaaaaa
     7921 atgaataaga ctgtccattt gtttattata gttaatgaaa tcacacaaat gcactactat
     7981 agtagtacaa attaaagtgg cattttacgc tattttcaaa ataaaatcag ctaattattt
     8041 gtatttgcaa atttttcgcc taaaacaacc gcttgtctcc aacatatttc gtctttcctg
     8101 ctttttcttg cgttagctca aatttaattt tattcattgc agtaaaataa gcgaaacatt
     8161 ctcttcactc gaacattcta tgttaaataa atggttttta actaaaaatg cttttcttgc
     8221 cgtcaagcct tttagtgcat taaaagatgc tctcaatgca ggttatggta aacaacattt
     8281 aatcagagat attatttcag gcttaaccgt tggcgttatt gctattccgt tatctatggc
     8341 gttggcgatt gccagcggtg tgccaccaca gcacggttta tatacggcga ttgttgccgg
     8401 gattgtgatt gccctaacag gcggttcaag atttaatatt tcaggcccaa cagccgcttt
     8461 tgtggtgatt ctataccccg tcacgcagca gtttggctta agtggattac tcatggcaac
     8521 aatgctttcc ggaggaattt tagtattgat ggcgttactc cgattaggac gcttaattga
     8581 atacattcct ctacctgtca cactgggttt tacctgtggc atcggtattg tgatcgccac
     8641 cttgcaaatc aaagattttc tgggcttaag cattgaaaaa atgcctgaac actatcttgg
     8701 caagctagag gctatttttt cagcattgcc aaccataaat tgggctgaca ccttagtggg
     8761 tgtcgtaaca ttgttggttt taactcgttg gcacaagcta cggttgccta ttcccgggca
     8821 tttacccgcc gtctttatcg gcacgctgtt atcattactg ctgattcatt tcggacaaaa
     8881 cgttgccact atcggctcag cattccacta cacgctctct gacggcacca ctgggtatgg
     8941 tatcccaagc gtattacctg aatttgtgtt accttggaat ctcccgaatg cagaggggaa
     9001 tcggatagat tggaacttaa ccaccattca agccttatta ccggctgcct tttcaatggc
     9061 aatgcttggg gctatcgaat cgttattatg tgcggtagtg tgcgataata tgacagatac
     9121 caagcaccac tctaacaacg aactattagc ccaaggctta ggtaatattg cctcgccatt
     9181 tttaggggga attaccgcca cagcagcgat tgcgagatcg gctgccaatg ttcgttccgg
     9241 ggcagtttcc cccatttcta gtgtggttca cgccttgttg gttttatttg ccctactctt
     9301 tttggctaaa gcactttcct atttgcctct ttcctcaatg gctgcactat tattggtagt
     9361 agcttggaat atggcagata ttccacaaat cattcaactt acccgccgtt caggtaaaaa
     9421 tgaaattgcg gttttactga cttgcattac cctcacggta ttatttgata tggtaattgc
     9481 cattacggta ggggtgttac tggcaagcct gctatttatc cgtactattg cagaaatgac
     9541 taaatccttt gaaatggctc acccaaccga tttgaacgat gtgttggttt atcggatcag
     9601 cggtccgtta ttttttgctg ccgccgataa tctctttgcc gatctgcaca ataaaaccgt
     9661 acataccgat caccaaatta aacatattgt gctgcaatgc gatgccgtta cagtattaga
     9721 tgctggtggc attcacgccc taacccgctt tgtacagcat atgttgccac atcaacaact
     9781 tcatctctgt aatgtgcaat ttcagccgat gagaatgcta gtccgctcac aaaaaacggt
     9841 aaacgaaatc ccgcaaattc aattcagtag cgatcttgaa tctgccttcg acaaaatcag
     9901 acaggctaac taggaataac tccggatata aaacaagcgg tcatattttc caactttttg
     9961 caaattgcaa cattttccga aaatatgacc gcttgtgttg aacgttagcc tttagccaaa
    10021 atctctttta agaagcgtgc ggtgtgagaa cgtttgtctt ttgccacttc ttccggtgtg
    10081 ccggtagcaa taatttcacc gccgccactg ccaccttcag ggccaagatc gacaatccag
    10141 tctgcggttt taatcacatc taaattatgt tcgatcacca caatcgtatt gccttgatca
    10201 cgcaggcgat gtagcacgcc gagtaactgt ttaatatcgg caaaatgcaa gccggtggtc
    10261 ggttcatcta aaatatagag cgttttgcct gtgtctcgtt tggaaagctc ggtcgccaat
    10321 ttcacccgtt gggcttcacc gcctgagagc gtagtagaag attgccctaa acggatataa
    10381 gataacccca cgtccatcaa ggtttgcaac ttgcgggcaa tcatcggcac aggggcaaaa
    10441 aattctctcg cctcttccac cgtcatctct aacacttggt gaatagtttt gcctttatag
    10501 cgaatttcca aggtttcacg gttatagcgt ttgcctttac agtgatcgca aggcacatac
    10561 acatcaggca agaagtgcat ttcaacttta atcacgccgt caccttgaca ggcttcgcaa
    10621 cgtccgcccc gcacattaaa gctaaaccgc cctacgttat accctctggc tcgagcttct
    10681 tgcgtgccgg caaaaagctc acgaattggc gtaaataatc cggtgtaagt tgccggattt
    10741 gagcgaggag ttctcccaat cgggctttgg tcaatatcaa ttaccttgtc aaaatgcgat
    10801 aagccgtcaa tcgattcata aggagcaaca tcgctgtttt ccgctcggtt taacgcattc
    10861 tgtgccagaa ggaatagtgt gtcattaact aaagttgatt tgcccgaacc tgatacaccc
    10921 gtgatacaag taaataaacc aaccggaatc tctaatttca cttttttcaa gttgttacca
    10981 cttgctcccg ataaggtgag caatttagtt ggctcataag gcacacgctg agccggaatc
    11041 tcaatttttt gcttgcctga gaggaattgt ccggtaatag attcctcaat ttgcataatt
    11101 tcagctgccg taccttgtgc gataacttgc ccaccgtgta caccggcacc cgggcctata
    11161 tccacgatat gatctgcggc tcggatcgca tcttcatcgt gttcaaccac aatcacggta
    11221 ttgcctaaat tgcgtaaatg caccaaagta ttcagtaggc gttcattgtc tcgttggtgc
    11281 aagccgatgg aaggctcatc cagcacatac attaccccta ctaaacccgc accaatttgg
    11341 ctggcaaggc gaatccgctg agcctcgcca cctgaaaggg tttctgccga gcgggaaaga
    11401 gaaagataat tcaagccgac attcactaaa aactgtagcc gttcacgaat ttctttgagg
    11461 attttctcag caattttttc tttctgaccg gctaatttca gctcttcaaa aaaggttaat
    11521 gcctcgccga tacttttttc cgaaaccatc ggcaaattgg ttttatctaa gaatacataa
    11581 cgggcttcac ggcgtaaccg tgagccttcg caatccatac aaggacgatt attaatatac
    11641 ttcgccaact cttcacgcac cgcgtttgat tcggtttctt tatagcggcg agccatatta
    11701 ttcagcaccc cttcaaaagc gtgtttacgt ttgaccttat cgcctcggtc attgacataa
    11761 acaaactcaa tctcttcttt agtgccgttt aaaataatgt tttgatattt tttcggtagc
    11821 tgcccgaatg gggtttctaa atcaaagcca taatgctcgg caacggcttt taataagcca
    11881 aaatagtaga aactgcggcg atcccagcct ttgatcgcac cggcggctaa tgaaacctct
    11941 tcattttgca ctactttttt ggcatcaaaa aactgctcaa cgcccaaacc atcacaagtc
    12001 gggcaagcac ctgccgggtt gttaaacgag aaaaggcgtg gttctaattc gtgtaaagag
    12061 taaccacaat gcggacaagc aaagctggac gaaaacacaa tttcttcggc atttgggtcg
    12121 tccatatctg caataattgc ggtagagccg gataaatcta acgctgtttc aaaagattcc
    12181 gccaaacggg tggctagatc agcccttact ttaaagcgat ccaccaccac ttcaatggtg
    12241 tgttttttct gtaattctaa tgccggtggg tcggataaat cgcaaatttc accgtcaatt
    12301 ctagcccgca aatagccact agcggtcaaa ttttccaata attttacgtg ttcgccttta
    12361 cgctctttca ccacaggggc aagtagcatc atccgttttc cttccggttc ttccaatact
    12421 cgatccacca tctgcgaaat agtttgagca gccaatggca catcgtggtc agggcaacga
    12481 ggctcgccca ctcgagcaaa caataaacgt aaatagtcgt gaatttccgt taccgttcct
    12541 acggtcgaac gtgggttgtg agaggttgat ttttgctcaa tcgaaatcgc aggggaaagc
    12601 ccttcaatat gatccacatc aggcttttcc attaaggaga gaaactggcg ggcgtatgca
    12661 gacagtgatt ctacataacg gcgttgtcct tccgcataga gcgtatcaaa cgccagagac
    12721 gatttgcccg agccggaaag cccggtaatt acaatgaatt tatctctcgg caaggttaaa
    12781 ttgatgtttt ttaagttgtg tgttcttgct ccacgaatat cgatatgttg catattttta
    12841 atgtccagtg ctattttctc aattttcgat cattatcgca tattttaata aaactgtaca
    12901 aaatatcagt tttttggtgt tcatcttttg gattttcagg tagaatactt agcaaatttt
    12961 agtttaattt atcaaacaga ggattttatg gctggtatca ataaagtaat tattgtcggt
    13021 aatttaggta acgaccctga aatgcgtaca atgccaaatg gcgaagcggt tgcaaatatt
    13081 agtgtggcaa cctcagaaag ctggacggat aaaaacaccg gtgaacgccg tgaagtaacc
    13141 gagtggcatc gtattgtatt ctaccgccgt caagcagaag tatgcgggca atatttacgt
    13201 aaaggctctc aagtttacgt agaaggacgt ttaaaaaccc gtaaatggca agaccaaaac
    13261 gggcaagatc gttacaccac cgaaattcaa ggcgatgtgc tacaaatgct cggtagccgt
    13321 caaggtaatg atatgggcgg tcagcaaggc ggttggggaa attctgctcc tgcgggtaat
    13381 agctacaatc aaggcaacag caattatggt tacaaccaaa cccaaagtgc tgcaccgcaa
    13441 tctaaaccgg caccacagcc tgagccggca atgaacaatt ttgatgatga tattccgttc
    13501 taatcaaagt attgacttaa gtagctcatg ggttccatac accgcatttc gatgaaataa
    13561 aaagctactc atataaagcg cccctacctt actcaaaata atagggaaca cttccaagta
    13621 ataagtgttc cctattaatt aataaatcca cttttcattc gattaataag taacttattg
    13681 acagatttat tattgcccca tttctcacat aaacggctaa agttcccatc tatacgggtt
    13741 tataaataca atattttgag aaaaattgat ataggaagat aatagagaaa ttcttaatag
    13801 taaataagaa aagatattag agtagtgcca gtggtcggac tcgaaccgac acgctttatg
    13861 ggcggcggat tttgaatcgt ttgtgtagga ttgttcaaat catattcgca aatacacagt
    13921 tactacgatg tatccagcca atacatccat tgggattttc cgtccgaatt tcagacgttc
    13981 tagatcttac acattctttc aagtttgtaa agcctgaaaa tcatttttta gattgatttt
    14041 atttgaaaaa atctattttt tcttttaatt caaatttagt caaaacttca ccagatattt
    14101 ttttccgaaa aaccctctct acctatttta ctgtttacat aactgctctt gcaattgcta
    14161 taaatggata ttataatttc caaaaaacat aaaaatggta ataataatgc tcagtaaaac
    14221 aaaatcacca actctgtttc ctaagtacca aaatgccttg cttcaacttg gagaaaatat
    14281 taggctagcc tcaaaaagga gaatgtttac tcaaacatta ttggctcaaa gaactgggct
    14341 tagccgtgta acagtgagaa aaattatgaa tggtgatcca actgttgcga ttggatacta
    14401 cctaatggtt ttaggtgttc tcggtctaga acaagatttc actgatgttg gaaaggatga
    14461 tgagttagga cgtaaattgc aagatattga attattaagg aaataatgat gaataaacga
    14521 atttatgtgt ttgcagattg ggaaactttt tcacagcctg aattagttgg ggtgctttct
    14581 tctgatgtag tgagaggcaa agaaaatttc cgttttagct acgataacga gtggttaagt
    14641 tcaccctatg tacagcagat tgatcctaac ttataccttt accctggaga gcaatacagt
    14701 acagatacac aaaattttca tatcttttta gattcttgcc ctgatagatg gggtagattg
    14761 ttaatgaaac gtcgggaggc agctctagct agtcaagaga atcgtaaacc taagttacta
    14821 atagaaagtg attacctttt aggcgtacac gatatgaata gaatgggggc tttacgtttt
    14881 aaaactgaat tgaatggtcc atttttggac aataatactc gtcttgcagc accaccactg
    14941 tcttccttaa gagaattaga atttgcagca atacaacttg aaagcagtga taatgtggac
    15001 gatccggatt atttaaaatg gcttcatatg ttgatatcac cagggtcgtc actaggcgga
    15061 gctagaccaa aagcttcggt aatagatgaa ctaaatcaac tatggattgc caaatttcca
    15121 agtcggtacg atgattatga tattgctctt tgggaatatt tagcttatcg actggctatt
    15181 caagccaaaa ttgaaatggc tacttgtaga attgaacgct ttaaccaaga acaccatact
    15241 tttttgacac aaagatttga tcgacattgt ggaaaacgtc gccattttac gtcagcatta
    15301 acccagctgg gttattatga cggagactat gaggcaagct accttgagat tgctcaattt
    15361 ttaacagaaa gtggttcaaa tactcgaatt gacttggctc aactatggag aagaattgta
    15421 ttcaatattg ctatttcgaa tactgatgat catttgcgta accacggatt tatctacgct
    15481 caagaggggt ggatattatc tcccgcatac gatttaaacc cagtaccact atctcaagga
    15541 ttacatttaa acataacaga cagcgataac cgattggatt atcagctcgc ctttgatgtt
    15601 gctgactttt ttcaattatc taagcaacaa gccgtacaga tttatgatga agtactgaaa
    15661 gaagttaata attggcaaaa tgtcgcaaaa gaaattggta ttccacgcca agagcaagag
    15721 cagatgaaat ttgcatttaa tgtatagtaa ttgacttgtg gaaccaatca tcgtagtcta
    15781 tgttctaaaa aagtagcgtt accttaataa cgcaatgagt tgttggctca ttgtcaatct
    15841 gatattaatc taggatgtaa aggcctagcg gtggcaggca ccagaaagac ctgatactaa
    15901 tctagggtat aaagacctag cggtggcaga caccagaaca aaccaacatc ttataaaacc
    15961 attagtaaca cttgactttt ttctcaacca tatccgcaag gaagcgtagc cttaaaaacg
    16021 caaatgtagg aaactaatct aggcatatat aatgcctagc agatggcaga cat
//
LOCUS       DANKBV010000048        15657 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.048, whole genome shotgun sequence.
ACCESSION   DANKBV010000048 DANKBV010000000
VERSION     DANKBV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15657
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.048"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1417)
                     /locus_tag="PWY43_001239"
     CDS             complement(<1..1417)
                     /locus_tag="PWY43_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HDL2136122.1"
                     /translation="MYLFEPLDRIIQGYLPPEQIERVKRAYVIARDAHEGQTRSSGEP
                     YITHPVAVASIIAEMKLDQEAVIAALLHDVIEDTPYTEEELAAEFGQSVADIVQGVSK
                     LDKLKFRTRQEAEVANFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
                     ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYRVLKMAIASARGHRKELIQRIAD
                     DIRGRLEEVQIEARVYGREKHLYSIYQKMRQKDQHFHSILDIYAFRVVVDNVDNCYRV
                     LGQMHALYKPRPKRVKDYIAVPKPNGYQSLHTSMIGPHGVPVEVQIRTEDMDKMADMG
                     VTAHWRYKEGESHHNTTVQLKAQQWLQSIVELQSSAGNTAEFIDSVKSDLFSDDIFVF
                     TPKGRIVELPANATAIDFAYAVHSGIGDRCVGAMVDRNPYPLSEPLRTGQTVEIITEQ
                     GKRPNASWLNFVVSGKARAKIR"
     gene            complement(1527..1808)
                     /gene="rpoZ"
                     /locus_tag="PWY43_001240"
     CDS             complement(1527..1808)
                     /gene="rpoZ"
                     /locus_tag="PWY43_001240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2136123.1"
                     /translation="MARVTVQEAAEKIGNRFDLILIAARRARQLQLHTREPLVPEEND
                     KPTVIALREIEEGLITTDIMDQLENHDSIKQEAAEQEAISFLTEMSNEA"
     gene            complement(1911..2408)
                     /locus_tag="PWY43_001241"
     CDS             complement(1911..2408)
                     /locus_tag="PWY43_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2136124.1"
                     /translation="MAHFYEYLEFNSDEDRENQLEVYVEVKLSTDTEEKLQAVGVQGD
                     WLVMAEPYCPDCVEIVAYFQRMAKLNPNIKVKYVARKDNQERKHFDSDEQQQAVISAQ
                     KIPTIFDIRNGKTELVLSEFPQFLKDKMAANPEQFEQLKADFRMGKFGKEVEAELLAI
                     LLNTK"
     gene            complement(2433..3455)
                     /locus_tag="PWY43_001242"
     CDS             complement(2433..3455)
                     /locus_tag="PWY43_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2136125.1"
                     /translation="MSVTKKRLTLSDIAELSGVSKTTVSMILNGRGNDFRIKSETCQK
                     VKEIAKKHGYRANVYAKSLQAQRTNVIGLVIPDLTNYGFALTSRTLEKLCRENGLQLV
                     IACSDDTFEGEKKAIERLLDRQVDLLITAPTHNEPTYYQNAAQYTPMLQLDRYISGLN
                     IPSITSNDTQSISMLVENIVRAYHLKEFFYLGGQLSLSPSQNRLKGFREGLEQSHLAL
                     DENWVLHQDYQPESGYQMFAQIVEKLGRLPEAVFTASFTLLEGVMRYLTEHQQMDKLL
                     TQELHLATFDDHHLLNALPFHIHSIKQDHQQIAERTFELIQQKLAQQKIDDVKVDCEI
                     LWRKSI"
     gene            complement(3495..4925)
                     /locus_tag="PWY43_001243"
     CDS             complement(3495..4925)
                     /locus_tag="PWY43_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL2136126.1"
                     /translation="MHIFNNGKYKSLHAQSEGELNALRQTVLSDPDFYPTYHLAPETG
                     LFNDPNGLIFDGEKYHIFAQWFPYGALHGMKHWQHFITTDFQTFSKGDLLIPDELFES
                     HGCYSGGAILWQDKIVAFYTGNTRRPSDNARIPHQNIAIFDKSGKLLEKRCIIDQAPA
                     GYTEHVRDPKPYLTAEGKIRFVLGAQRENLTGTALIYEMDDLNATPRLIAELEVHNFD
                     NSNVFMWECPDLFSLGGKDLFVWSPQGKLRESHQFQNNYHATYALGELNGNRLRAEHI
                     EELDYGFDFYAPQTVENSDRILFGWVGLPDLTYPTDKYQWHSMLTLPRKLSLQNGKVI
                     QNPIVNVGELQAVEMSENFAIENLDTAYLQISVENQPLVLDFFSNEQGQKLSLRFENG
                     VLSLDRSQTEQTELMEKFGSVRHCKIEQLETIEIFFDRSVVEIFVNGGEKVLTSRFFI
                     AKRENVVKSSRPIQVKVAKVLPISVE"
     gene            complement(5030..5953)
                     /locus_tag="PWY43_001244"
     CDS             complement(5030..5953)
                     /locus_tag="PWY43_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDL2136127.1"
                     /translation="MSKIWVTGDAVVDLIPDGEHHYLKCAGGAPANVAVGVSRLGVEA
                     GFIGRVGNDPLGKFMREVLQAENVCTKQMILDAQHRTSTVIVGLDNGERSFTFMVNPS
                     ADQFLEIGDLPEFNQGDFLHCCSIALINDPSRSTTIEGIRRVKKAGGYVSFDPNLRES
                     LWKSLDEMKTVVNSVVAMADILKFSEEELTLLTETETLEQATQAITAQYPEKLIIITL
                     GKDGAIYHFNGKSQVVAGKALKPVDTTGAGDAFVSGLLAGLAQTPNWHDETVLVEVIR
                     KANASGALATTAKGAMSALPNKAELDAFLAE"
     gene            complement(6070..7497)
                     /gene="miaB"
                     /locus_tag="PWY43_001245"
     CDS             complement(6070..7497)
                     /gene="miaB"
                     /locus_tag="PWY43_001245"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDL2136128.1"
                     /translation="MAKLHITTWGCQMNEYDSSKMADLLNSTHGFELTENPEDADVLL
                     LNTCSIREKAQEKVFSQLGRWKNWKKDKPNLIIGVGGCVASQEGEHIRDRAPYVDIVF
                     GPQTLHRLPEMINQIRGGKSSIVDISFPEIEKFDRLPEPKAEGPTAFVSIMEGCNKYC
                     SFCVVPYTRGEEVSRPVDDVLFEIAQLAAQGVREVNLLGQNVNAYRGETFDGGICTFA
                     ELLRLVAAIDGINRLRYTTSHPIEFTDDIIEVYRDTPELVSFLHLPIQSGSDRVLTMM
                     KRNHTALEYKAIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFS
                     FIYSARPGTPAADLPDNVSDDEKKERLSRLQQRINHQAMQFSRAMLGTEQRILVEGPS
                     KKDLMELTGRTETNRVVNFVGTPDMIGKFVDVKITDVYTNSLRGEVIRTEDQMGLRVV
                     ESAESVIARTRKEDDLGVGKYVVNL"
     gene            complement(7714..8214)
                     /gene="crr"
                     /locus_tag="PWY43_001246"
     CDS             complement(7714..8214)
                     /gene="crr"
                     /locus_tag="PWY43_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005702996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2136129.1"
                     /translation="MGLFDKLFGSKQKESQEIKIYAPLTGEIVNIEDVPDVVFSEKIV
                     GDGVAIRPVGDTIVSPVNGTIGKIFETNHAFSIESEEGVELFVHFGIDTVELKGEGFT
                     RLAQEGQTVKVGDPIIKFDLALLEGKAKSVLTPIVISNMDEISNLSKLNGQVVAGESV
                     VLTLTK"
     gene            complement(8299..10020)
                     /gene="ptsI"
                     /locus_tag="PWY43_001247"
     CDS             complement(8299..10020)
                     /gene="ptsI"
                     /locus_tag="PWY43_001247"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDL2136130.1"
                     /translation="MITGIAASPGAVFGKALVLKEEPIVLNTRKITESEVEAEKAKFF
                     TGREKAAAQLTAIKEKARRTLGEEKEAIFEGHLMILEDEELEEEILSYLTDNLVTADV
                     AASKVIEMQASMLAEIDDEYLKERAGDIRDIGNRLVRNILNMNIVDLGDIQDEVILVA
                     YDLTPSETAQLNLDKVLGFITDIGGRTSHTSIMARSLELPAIVGTNNVTSLVKTGDFL
                     ILDAVNNEVHLNPSEEKIANFKALQEKLNAEKEELAKLKELPAQTLDGHKVDVAGNIG
                     TIRDVEGVLRNGGEAVGLYRTEFLFMDRTQLPGEEEQFQAYKEIVEAMNGQMVILRTM
                     DIGGDKELPYLDLPKEMNPFLGWRAVRIGFTRREILDTQLRAVLRASAFGNLAVMFPM
                     IISVEEIRLLKSIVAELKDQLRAEGHAFDENLKLGIMVETPSAAVNARHLAKEADFFS
                     IGTNDLTQYTLAVDRGNEIIAHLYNPLSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     VRATALLLGMGLDEFSMSGISVPHVKKLIRSINFADAKALADEALAQPTAADIERLVD
                     EFYQKLN"
     gene            complement(10142..10399)
                     /gene="ptsH"
                     /locus_tag="PWY43_001248"
     CDS             complement(10142..10399)
                     /gene="ptsH"
                     /locus_tag="PWY43_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDL2136131.1"
                     /translation="MYSKDVVITAPNGLHTRPAAEFVKAAKGFASDITVTSGGKSASA
                     KSLFKLQTLGLTQGTTITISADGEDEQKAVDFLVDLIPTLE"
     gene            10660..12234
                     /locus_tag="PWY43_001249"
     CDS             10660..12234
                     /locus_tag="PWY43_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDL2136132.1"
                     /translation="MLSTLTKTTAWFIYFSIIALYVFSLWALMDYHQNDTLFVFSDIT
                     ATVAYSLLQASLSAVISLFLGSLLARSFFYLDFKGKNLLYKTISFIWALPSLVIIFAV
                     IGVFGNSGWLAKLFQQLGWNWQFNLYGLHGIILAHCLFNIPFVMKYYTGGLQLTPSSR
                     FQLAAQLNLTGWNYIQIVELPIIKTLLPYMFMTVFLVCFTSFPIVLMLGGSPKYSTLE
                     VAIYQAVTFEFDFAKALILIGVQFVVGIGLQLVMNYISTSALKKLKQQPNRVEIWKPK
                     PTGLAKLGLQAVIFLQSFAIILPLVSVIWAGLSVSKLSERLLNPTLWQATQYSLYLSL
                     IASIFVITISYLIALEARQLLYAKAKLRYSILTAITLFPLLLPIFLLSVGLFILLMNS
                     ELSSEALLLLVGICNGLTLLPFIYPMLFSAMWNSLTSHDKLAQGLGLKGLRRWWIVEK
                     NQLIRPLVTVFALAMSSSLGSFTVIAFFGNADFSSLPYLLYQQLGSYRTEDAAVTALI
                     LMLFALLPFLFSKDKL"
     gene            12231..12881
                     /gene="thiQ"
                     /locus_tag="PWY43_001250"
     CDS             12231..12881
                     /gene="thiQ"
                     /locus_tag="PWY43_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HDL2136133.1"
                     /translation="MIELDLHFTYEQMNMHFQLSVKKGERIAVVGESGAGKSTLLNLI
                     AGFEKAESQQSQLWLNQKNHLDTEVAQRPVSMLFQDNNIFPHLTVEQNIGLALVPSLS
                     LNPKQKAQVKQIATQMGIETFLQRQADQLSGGQKQRVALARTLLRDKPILLLDEPFSA
                     LDPQRRAELQQIVFDICQSRNLTLLMVTHQLSESLFLFNRVITVADGEIVSDLSPL"
     gene            13188..14606
                     /locus_tag="PWY43_001251"
     CDS             13188..14606
                     /locus_tag="PWY43_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDL2136134.1"
                     /translation="MNYPKIAEQTIAKLGGKENITTLAHCATRLRLTIADESKIDKEA
                     IENIEGVKGQFSTSGQYQIIFGSGTVNEVYAQMQKQMGMADMSTSEVAAAGAANQPLL
                     QRLVKGLADIFVPIIPAIVAGGLLMGIHSMLTAVGFFWEGESVVSRYPNISDLVDFIN
                     TIANAPFVFLPVLLGFSATRKFGGNPFLGAALGMLLVHPALADGWNYAKTLMEGNIKY
                     WNIFGLEIEKVGYQGTVIPTIVSAWVLATLEKTFRKFVPSYLDNLVTPMMSLFIAGIL
                     AFTVIGPLGREAGSAISAGLTWLYDSLGFIGGAIFGTFYAPIVITGMHQTFIAVETQL
                     LAEVAQTGGTFIFPIAAMSNIAQGAACLGVAFAMKDAKVRGLAVPSGISALLGITEPA
                     MFGVNLRYRQAFIAAMIGSGLASAFIAFFNVKAIALGAAGFLGIPSIKPDSLAMYSVG
                     MVISFVVAFTISAIWVKKAQAK"
     gene            complement(14722..15525)
                     /gene="map"
                     /locus_tag="PWY43_001252"
     CDS             complement(14722..15525)
                     /gene="map"
                     /locus_tag="PWY43_001252"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2136135.1"
                     /translation="MNNIPLRNEEEIQKLRVACKLASDVLVMIAPYVKEGVTTGELDK
                     ICHEYILANGATSASLGYHGFPKSVCISLNEVVCHGIPSDDKKLKKGDILNIDVTVIK
                     DGYYGDNSMMYVVGEPSVRDKRLMAVTLESLYVGIRTVKAGVRLKEIGTAIQKYVEKE
                     GFSVVREYCGHGIGTEFHCDPQVVHYATDDGGVVLKEGMVFTIEPMVNAGKKEVRLMG
                     DGWTVKTKDRSHSAQYEHQIVVTKDGCEVLTIRDEEIAEGRISRIMHNS"
BASE COUNT         4394 a         3511 c         2937 g         4815 t
ORIGIN      
        1 gacgaatttt agccctcgcc ttaccactta ctacaaaatt taaccacgat gcatttgggc
       61 gcttaccttg ctcggtaata atttccaccg tctgtcctgt tcgtagcggt tctgaaagtg
      121 gatacggatt gcgatcaacc atcgcaccta cacaacgatc cccaatgcct gaatgcaccg
      181 cataggcaaa gtcaatagcg gtagcattcg ccggcaattc aacaatacgc cctttcgggg
      241 taaagacgaa aatatcgtca gagaacagat ccgatttaac gctatcaata aattctgctg
      301 tattgccggc actgctttgc aattcaacaa tactttgtag ccattgctgg gctttgagtt
      361 gaacggtcgt gttatggtgc gactcgccct ctttgtaacg ccagtgagct gttaccccca
      421 tatccgccat tttgtccata tcttcggtgc gaatctgtac ctccaccggt acaccgtgcg
      481 ggccaatcat cgaagtgtgt aaagattgat agccattcgg ttttggcacg gcaatataat
      541 ctttcacccg tttcggacga ggcttataca gtgcgtgcat ctgccctaat acacggtaac
      601 aattatctac attatcaacc actactcgga aagcataaat atctaaaatc gaatgaaaat
      661 gttgatcttt ctggcgcatt ttttggtaaa tggaatagag atgtttttca cgcccataca
      721 cacgggcttc aatttgcacc tcttctaacc gaccacgaat atcatcggca atgcgttgaa
      781 ttagctcttt acgatgtcct ctggcactgg caatagccat tttcagtacc cgataacgtt
      841 gcgggtgcat tgcctcgaag cctaaatctt ccaactcgtt tttaatatgc tctattccca
      901 agcgatgtgc caacgggcta taaatctcta aggtttcttt cgctatacgg cggcgtttat
      961 ccggacgcaa agcaccaagc gtacgcatat tatgggttcg gtcggcaagt ttaattaaca
     1021 caacccgaat gtctttggtc attgccaaaa tcattttacg gaagtttgcc acttctgcct
     1081 cttggcgggt gcggaatttt aatttatcca gcttagaaac gccttgtaca atatctgcta
     1141 cgctctgtcc aaattccgcc gccagttctt cttcggtgta aggtgtatct tcaatcacat
     1201 cgtgcaacaa agccgcaatc accgcttctt gatcgagctt catttccgca ataatcgacg
     1261 ccactgctac cgggtgagta atataaggct caccgctaga gcgtgtttgc ccctcgtgag
     1321 catcacgggc tatcacataa gcacgtttta cacgctcaat ctgctcgggt ggcaaatagc
     1381 cttgaataat tcgatctaaa ggttcaaaaa gatacacgtt atacctcaac aacagaaata
     1441 aaaatggaca acgaatttgc aaattttgag gaaaatccta ccgcttgcag actcaattgt
     1501 ccattctcta tttacgaaaa ataaaattac gcttcgttgc tcatttcagt taaaaatgaa
     1561 atcgcctctt gctccgccgc ttcttgctta atagagtcgt gattttccag ctgatccata
     1621 atgtcagttg taattaagcc ttcttcaatt tcacgtaatg caatcacggt cggcttatcg
     1681 ttttcttcag gcacaagtgg ctcacgggtg tgaagttgta attgtcttgc acgacgtgca
     1741 gcaattaaga ttaaatcaaa acggttgcca attttctccg ctgcttcttg tacggttaca
     1801 cgagccataa atttactcca aagatttatt cggttttatt cataaaaaga ttgcgaatta
     1861 tacagaaatt ccgccaattc cgcaatgaaa gcgggctaaa ttcacaaaat ttatttggta
     1921 ttcagtaaaa tcgccaataa ttcagcctca acctctttgc cgaattttcc cattctaaaa
     1981 tcggctttta gttgctcgaa ttgctcaggg ttcgctgcca ttttatcttt taaaaattgc
     2041 ggaaattcag ataacactaa ttctgttttg ccattacgaa tatcaaaaat agttggaatt
     2101 ttttgcgcag aaatgaccgc ttgttgctgt tcatcgctgt caaaatgttt gcgttcctga
     2161 ttatctttgc gagccacata tttcacctta atgttaggat tgagctttgc cattcgctgg
     2221 aaataagcca caatttccac acaatcgggg caatatggct ctgccattac cagccagtcc
     2281 ccttgcacgc caacagcttg cagtttttct tccgtatctg tggaaagctt gacctccaca
     2341 tacacttcta attgattttc acgatcttca tcgctgttaa attctaaata ttcgtaaaaa
     2401 tgtgccataa ttttgtcttt tcctatctca tcttaaatgg atttacgcca taaaatttca
     2461 caatcaactt taacgtcatc tattttctgt tgcgctaact tttgctgaat taattcaaag
     2521 gtacgttcgg caatttgttg gtggtcttgc ttaattgaat gaatatgaaa aggtagtgca
     2581 ttcagtaaat ggtgatcatc aaaggttgct aaatgcagtt cttgagtcaa taatttatcc
     2641 atttgttggt gttcagtcag atagcgcatt actccctcaa gtagggtaaa agaggctgtg
     2701 aataccgctt ctgggagcct gccaagtttt tcaactatct gagcgaacat ttgatagccc
     2761 gattccggct gatagtcttg atgcagaacc caattttcat caagtgccaa atgtgactgc
     2821 tccaaacctt cacggaagcc ttttaaacga ttttgacttg gcgagagtga aagttgtcca
     2881 cctaagtaaa aaaactcttt taagtgataa gcccgtacaa tattttccac caacatactg
     2941 atactttgtg tatcattact ggtgatagaa gggatattca acccagaaat atagcggtct
     3001 agctgtaaca ttggggtata ttgtgccgca ttttgataat aagtcggctc attatgtgtc
     3061 ggagcagtaa tcagtaaatc tacctgtctg tctaataaac gctctatcgc tttcttctca
     3121 ccttcaaagg tatcatccga gcaagcgatc actagctgta acccgttttc acggcataat
     3181 ttctctaagg ttcttgaggt cagagcaaag ccataattcg ttaaatcggg aatgactaaa
     3241 ccgatcacat tcgtgcgttg agcctgtaat gattttgcat atacattagc acgataaccg
     3301 tgttttttag cgatctcttt cactttttgg caggtttcgg atttgattct aaaatcattg
     3361 ccccgcccat ttaaaatcat actgactgtt gttttagata cgccgctgag ttccgcaata
     3421 tcactgagcg ttaggcgttt tttagtcaca cttatctcct tttagcataa aaatgggcat
     3481 tactgcccat tgctttattc tacgctaatt ggcaatactt ttgctacttt cacttgaatc
     3541 ggacgagagc tttttacgac attttcacgc ttagcgataa aaaagcgtga agtcagcact
     3601 ttttccccgc cgtttacgaa aatttccacc acagaacggt caaagaaaat ctcgattgtc
     3661 tctaattgtt caattttgca atgacgaacc gagccaaatt tttccatcaa ttctgtttgt
     3721 tcggtttggc tacgatctaa gctcaacacc ccattttcaa aacgtaagct gagcttttgc
     3781 ccctgttcat tgctgaaaaa atccaacaca agcggttgat tttccacaga aatttgcaag
     3841 taggcagtat ccaaattctc gattgcaaaa ttttctgaca tttcgaccgc ttgtaattcg
     3901 cctacattca cgattgggtt ttgtatcact ttgccgtttt gcagggaaag tttacgaggc
     3961 aatgtcagca ttgaatgcca ctgatattta tcagttgggt aagtgagatc aggcaagccg
     4021 acccagccaa acaaaatgcg atcggaattt tccaccgttt gcggtgcgta aaagtcaaag
     4081 ccgtagtcca gctcttcaat atgctctgcc cgtaatctgt tgccgtttag ctcgccaagt
     4141 gcgtaagtgg cgtggtagtt attttggaat tgatggcttt cacgcagttt gccttgtggc
     4201 gaccacacaa acagatcttt accaccaagt gagaacagat ccggacactc ccacataaac
     4261 acattgctgt tatcaaaatt gtggacttcc agttctgcga ttaaacgggg tgtggcattt
     4321 aaatcgtcca tttcataaat taacgccgtg ccggtcaagt tttcacgctg agcccccaac
     4381 acaaaacgaa ttttgccctc tgcggtaagg taaggtttcg ggtcacgcac gtgttcggtg
     4441 tagcctgccg gtgcttggtc gataatgcaa cgcttttcca ataatttgcc cgatttatca
     4501 aaaatcgcga tattttggtg aggaatgcgg gcattatctg atgggcggcg tgtattgccg
     4561 gtgtaaaaag cgacaatctt atcttgccat aaaatcgccc cacctgaata acagccgtgg
     4621 gattcaaaca gctcatcggg aatcagcaaa tcccctttac tgaacgtttg aaaatcggta
     4681 gtgatgaaat gctgccaatg tttcattcca tgtaatgccc cataaggaaa ccattgggcg
     4741 aaaatatggt atttttcacc atcgaaaatc aagccgtttg ggtcattaaa taagcctgtt
     4801 tcgggagcga gatgataggt tgggtaaaaa tcgggatcag ataaaacggt ttggcgaaga
     4861 gcgtttaatt cgccctcgct ttgtgcgtgc aggcttttgt atttgccgtt gttgaaaatg
     4921 tgcatagagt gttcctgtca tttcagaaat tacgatggtg caagcgtcca cgcttgtacc
     4981 cttttgtaaa aaatgaggca caagcgtgga cacttgcgcc atcattacat tattccgcca
     5041 aaaatgcgtc taactccgct ttattcggta atgccgacat cgcccctttt gctgtggtgg
     5101 ctaacgcacc ggaagcattt gctttgcgga tcacttcaac taacacagtt tcatcgtgcc
     5161 aattcggcgt ttgagcaaga ccggctaata atccgctgac aaaggcatct ccggcaccgg
     5221 tggtatccac aggtttcaag gctttgccgg caacgacttg gctttttccg ttgaaatggt
     5281 agattgcacc gtctttgcca agggtgataa taatcaattt ctccggatac tgagcggtaa
     5341 tagcttgtgt ggcttgttcc aaagtttctg tctcggttaa taaggtcagc tcttcttcgg
     5401 aaaatttgag aatatccgcc atcgccacca cagaatttac cactgttttc atttcatcaa
     5461 ggctcttcca gagcgattca cgcaagtttg gatcaaacga aacatagcca cccgcctttt
     5521 tcactcggcg aatcccttca atggtagtgg aacgggatgg gtcgttaatc aaggcaatag
     5581 aacagcagtg taagaaatca ccttggttaa attcaggtaa atcgccgatt tctaaaaatt
     5641 ggtcggcact cggatttacc ataaaagtaa aactacgctc accgttatct aagccaacaa
     5701 tcacggtgga agtgcggtgc tgagcgtcta aaatcatctg tttggtgcaa acattttctg
     5761 cctgtaacac ttctcgcata aatttaccca gagggtcgtt acccacacgc ccgataaatc
     5821 ctgcctcaac ccctaaacga gaaacgccta ctgcaacatt cgccggagca ccaccggcac
     5881 attttaaata gtgatgttcg ccgtcgggaa ttaaatccac caccgcatcg cctgttaccc
     5941 aaatcttact catctcatac acctttaatt agtcacaaaa caccaaacta aaacgattta
     6001 gcatttattc gattatatac taaaagaccg cttgtacata cctacaagcg gtcatttttt
     6061 ctttaatttt tacagattaa ccacgtattt gcctacacct aaatcatctt ctttacgagt
     6121 gcgggcaatc acgctttctg ccgattccac cacacgtaat cccatttggt cttcggtacg
     6181 aatcacttcg ccacggagtg agttggtata aacatcggtg attttcacat cgacaaattt
     6241 gccaatcata tccggtgtgc caacaaagtt tactacacgg ttagtttcgg ttctgccggt
     6301 taattccatc aagtctttct tagacgggcc ttcaaccaaa atgcgttgtt ctgtaccgag
     6361 cattgcacgg ctaaattgca ttgcttggtg gttgatgcgt tgttgtaaac gggataatcg
     6421 ctcttttttc tcatcatcgg agacattatc aggcagatct gccgctggtg tacccggtcg
     6481 agccgagtag ataaagctaa agctcatatc aaagttcact tgctcgatca ctttcattgt
     6541 ttgctcgaaa tcctctgcgg tttcgcccgg gaagcctaca ataaagtccg aactaatttg
     6601 aatctcaggt cgaacagcac gcaatttacg gataatcgct ttgtattcca acgctgtgtg
     6661 gttacgcttc atcattgtta atacacggtc tgaaccactt tgaatcggta aatgtaagaa
     6721 actgaccaat tcaggtgtat cacgataaac ttcgataata tcatcggtaa attcaatcgg
     6781 gtggctggtg gtgtaacgta agcggttaat accgtcaatc gctgccacta agcgtaataa
     6841 ttcggcaaag gtacaaatgc cgccatcaaa agtttcacca cggtaagcat ttacgttttg
     6901 accgagtaaa ttaacctcac gcacaccttg tgccgcaagt tgggcgattt caaataatac
     6961 gtcatccacc gggcgggaaa cctcttcgcc acgagtgtaa ggcacaacgc agaacgaaca
     7021 atatttattg cagccttcca taattgaaac gaatgctgta gggccttcgg cttttggctc
     7081 cggcaagcgg tcaaattttt caatttccgg gaaagaaata tcgacaatcg agcttttacc
     7141 gccacggatt tggttgatca tttcaggcaa acggtgcaaa gtttgtggcc cgaaaacaat
     7201 atctacataa ggggcacgat cacggatatg ctccccttct tgtgaagcca cacaaccgcc
     7261 aacaccgata atcaggttcg gtttatcttt tttccagttt ttccaacgcc cgagttgcga
     7321 gaatactttt tcttgtgctt tttcacggat agaacaagtg tttagcaaca gtacatccgc
     7381 atcttctgga ttttcggtca attcaaaacc gtgagtggag tttaggagat ccgccatttt
     7441 tgatgagtcg tactcattca tttgacagcc ccaagtggta atatgtaatt tagccataac
     7501 gtttaaaaat aataaataat tcaataaatt agaaaagaaa agcgtaaagc aatttattat
     7561 ttttgactta aaataaaccc tttacgcaaa atttatggcg ttattttacc ttgttgccta
     7621 ttttttatct ataaataatc gccatttatc tgattttact taaaaacaaa aagccacgat
     7681 cttctatcgt ggcttcaaga taagtatgcg gtattacttg gttaaggtta aaaccacgct
     7741 ttcaccggca acaacttgac catttaattt agataagttg ctgatttcat ccatatttga
     7801 aatcacgatt ggcgttaata ctgatttggc tttaccttca agtagagcta aatcaaattt
     7861 gatgattgga tcgcctactt ttaccgtttg accttcctga gcaagacgtg taaaaccttc
     7921 gccttttaat tccaccgtat caataccaaa gtgaacaaat aattctacac cttcttcaga
     7981 ttcaattgag aaagcatggt ttgtttcaaa aatcttacca atagttccat ttaccggcga
     8041 aacaatcgta tcgccaaccg ggcgaattgc cacaccatca ccaacgattt tctcagagaa
     8101 aactacatca ggcacatctt caatatttac gatttcacct gttaatggcg catagatttt
     8161 gatttcttga ctctcttttt gttttgagcc gaataattta tcaaataaac ccatcttaaa
     8221 atctccctta gatattagat acaaaattct cttacatttt atacgagaat tttgtatttg
     8281 tatgctaaat aattaaaatt agtttaattt ctgataaaac tcatccacta agcgttcaat
     8341 atctgcagca gttggttgag caagcgcttc atcagctaat gctttagcat cggcaaaatt
     8401 aattgagcga atgagttttt taacgtgtgg cacagagata ccactcatac taaattcatc
     8461 taatcccata ccgagcaaca acgcagttgc acgcacgtca ccggctaatt caccacacat
     8521 acctgtccat ttgccttcag cgtgtgaagc atcaataact tgcttgatta agtttaagac
     8581 tgatgggctt aatgggttat ataaatgagc aataatttca ttaccacggt caaccgctaa
     8641 agtatattga gttaagtcat tagtgccgat actaaagaag tccgcttctt ttgcaagatg
     8701 acgtgcattt accgctgctg atggggtttc caccataata cctagtttta agttctcatc
     8761 aaaagcgtga ccttctgcac gtaattgatc ttttaattcc gcaacgatgg attttaataa
     8821 acggatttct tccaccgaaa taatcatcgg gaacattacc gctaaattac cgaacgcaga
     8881 agcacgcaat acagctcgta actgggtatc taaaatttca cggcgggtaa aaccgatacg
     8941 cacagcgcgc caacctaaga acgggttcat ctctttcggt aaatcaaggt atggtaactc
     9001 tttatcaccg ccgatatcca ttgtacgcag aatgaccatt tgaccattca ttgcttcaac
     9061 aatctctttg taggcttgaa attgctcttc ttctcccggt aattgagtac gatccataaa
     9121 caaaaactca gtacggtata aaccaaccgc ttcaccaccg ttgcgtaata caccttccac
     9181 atcacggata gtaccgatat tccctgccac atcaacttta tggccatcta atgtttgagc
     9241 aggcaactct tttaatttgg ctaattcttc tttttccgca tttaatttct cttgtaatgc
     9301 tttgaaatta gcgatttttt cttccgatgg atttaaatgt acttcattgt tcactgcatc
     9361 taaaatcaga aaatcaccag ttttaactaa ggaagtaaca ttgttagtac caacaatggc
     9421 aggaagctct agggaacgag ccataataga agtgtgagaa gtacgaccac cgatatcggt
     9481 aataaagcct aatactttat ctaaatttaa ttgagctgtt tcggaagggg ttaagtcata
     9541 agcgactaaa ataacttcat cttgaatatc accaagatct acgatattca tatttaaaat
     9601 attgcgtacc aagcgattac caatatcacg aatatcacct gcacgttctt ttaaatattc
     9661 atcgtcaatt tccgcaagca ttgaagcctg catctcaatc actttacttg ctgcaacatc
     9721 cgcagtcaca agattatcag taaggtaact caaaatttct tcttcaagct cttcatcttc
     9781 taagatcatc aagtggcctt cgaaaatcgc ctctttttcc tcacctaatg tacggcgagc
     9841 tttttcttta atcgcggtta attgagcagc tgctttttcg cggcctgtaa agaattttgc
     9901 tttttctgcc tcaacttcgc tttcagtaat tttgcgagta ttcagtacaa tcggctcttc
     9961 ttttaaaacc aatgcttttc caaatactgc accaggggaa gctgcgatac ctgtaatcat
    10021 aattgtgtcc ttaatttaag atgatgtaaa aatcatctgg atgttacata gcaaaacact
    10081 ttattgtagc ttattaagct acaataaagt gacataaatc ttaaatccga gaatagaaaa
    10141 attattctaa agtaggaatc aaatcaacta agaaatcaac tgctttttgc tcatcttcgc
    10201 cgtccgcaga aattgtaatt gttgtgcctt gagtcaaacc taatgtttgt aatttgaata
    10261 agctttttgc acttgcactt ttaccacctg aagtcacagt gatatcagaa gcaaaacctt
    10321 tagctgcttt tacaaattca gccgctgggc gagtgtgtaa accatttgga gcggtaataa
    10381 caacatcttt tgagtacata aaatcttcct cttaaatttg aaagttaaaa aaatttgttt
    10441 taatccattt attttgcgca cgcaacttta ctacaaattc aaattaaaaa cacgctaaaa
    10501 agcgcaaaaa ctcgtcgtat caccctgcta aatagggtgt tttttaagct aacttaaaaa
    10561 tcatttgtcg aatatcaaga ttctagcaca cacttaagcg gaaatgttac aataatttcg
    10621 ctaccacttc tttcacttat attctaaatt tggagcaata tgcttagcac attaaccaaa
    10681 acaaccgcat ggtttatcta ctttagtatt attgctctct atgtttttag tctatgggcg
    10741 ttaatggact atcaccaaaa tgatacctta tttgtttttt ccgatataac ggccacggtc
    10801 gcttatagcc tacttcaagc cagcctctct gcggtaattt cactattttt aggcagccta
    10861 ctggcaagat ctttctttta tttggacttt aaaggaaaaa atttactcta taaaaccatt
    10921 tcgtttatct gggcattacc ctcactagta attatttttg cggtaattgg cgtatttggt
    10981 aactcaggct ggctagccaa acttttccaa caactcggtt ggaattggca atttaattta
    11041 tacggcttgc acggcatcat tttggctcac tgcctgttta acattccgtt tgtgatgaaa
    11101 tattataccg gagggcttca attgactcca agcagtcgct ttcagcttgc ggctcaatta
    11161 aacctaacag gttggaatta tatccaaatt gtggaactac cgataatcaa aactttgctg
    11221 ccttatatgt ttatgactgt ttttctggtc tgctttacca gttttccaat cgtattaatg
    11281 ttaggcggta gcccaaaata cagcacgctt gaagttgcca tttatcaagc ggtcactttt
    11341 gaatttgatt ttgcaaaagc cctcatctta attggggtac aatttgtagt gggcattggc
    11401 ttacaattag taatgaatta tatctcgact tctgcactca aaaagctaaa acagcaacct
    11461 aaccgagtag aaatttggaa accaaagcct accggactgg ctaaactcgg cttacaagcg
    11521 gttatttttt tgcaaagttt tgcaataatt ctaccgcttg tcagcgtcat ttgggcagga
    11581 ctaagcgtgt caaaactcag tgagcgtttg ctcaacccta ctttatggca agctactcag
    11641 tattctttat acttaagttt aattgcttct atattcgtaa tcacaatcag ctatttgatt
    11701 gcacttgaag ctcgccaatt actctatgca aaagccaagc tcagatatag cattctcacc
    11761 gcaatcaccc tattcccctt acttctgccg atctttttat tatcggttgg tttgtttatt
    11821 ttattaatga atagtgaact ctcaagcgag gcattactgc tactggtcgg gatatgcaac
    11881 ggcttaacat tactgccatt tatttaccca atgcttttct ccgcaatgtg gaatagcctc
    11941 acctcgcacg acaaattagc acaaggactt gggctaaagg ggcttcgccg ttggtggatt
    12001 gtggaaaaaa atcaactaat tcgaccgctt gttaccgtat ttgcgttagc aatgtcatcg
    12061 agcttaggca gtttcaccgt cattgctttt ttcggcaacg ctgatttcag ctcactccct
    12121 tatttactct accaacaact tggtagctat agaaccgaag atgctgccgt taccgcactc
    12181 attctgatgt tatttgcgtt actaccattc ttattcagca aggataaact atgatcgaac
    12241 tggatctcca tttcacttac gaacaaatga atatgcactt tcagctttct gtcaaaaaag
    12301 gcgaacgtat tgcagtggtt ggcgaaagtg gcgcaggcaa aagcacatta cttaatctaa
    12361 ttgccggttt tgaaaaggca gaaagtcagc aaagccagct gtggctaaat cagaaaaatc
    12421 atcttgatac cgaagttgca caacgtcctg tttcaatgct attccaagat aacaatattt
    12481 tcccgcactt aacggtagaa caaaacatcg gcttagcttt agtaccaagt ttgtcgctca
    12541 atccgaaaca aaaagcacag gttaaacaaa ttgccacaca aatggggatt gaaaccttcc
    12601 ttcagcgtca agccgatcag ctatcgggcg gacaaaaaca gcgagtggca ttagctcgta
    12661 ctttgttgcg ggataagccg attttattat tggacgaacc cttctccgcc ctcgacccgc
    12721 aacgccgagc cgaattacag caaatcgtgt ttgacatttg ccaaagcaga aatctaaccc
    12781 tgttaatggt aactcaccaa ctatccgaaa gcctatttct atttaatcgg gtgataacgg
    12841 tagcagatgg ggagattgtt tctgatctct cccctcttta gcaaagcaga tctgctaggt
    12901 tctaaatgta gggacgccac gctggcgtac gcaacgatgt caattccaac aatctaacgg
    12961 tgaatgatgt tccgcacgga tactagcgtt attcattttt ttcagaactc gccccctact
    13021 ttagcaaaga ggggaactgt aactaatatt acctccctat tccgctattc tcttccccaa
    13081 aatgtgatct acttcacaaa tttggaaata ttttaaatct tctctttttt agctaaaccg
    13141 ttttagttaa aatgcaaccg attatttttt atgaaaggta aattcaaatg aactatccaa
    13201 aaattgcaga acaaacgatt gcaaaattag gtggcaaaga aaacattacc acacttgccc
    13261 actgtgcaac ccgtttacgc ttaaccattg ctgatgaatc caaaattgat aaagaggcga
    13321 ttgaaaatat tgaaggcgtg aaaggtcaat tctctacttc cggtcaatac caaattattt
    13381 ttggatcagg cacggtaaat gaagtgtatg cccaaatgca aaaacaaatg ggtatggcag
    13441 atatgtccac ctctgaagta gcggctgccg gtgcagcaaa tcagccatta ctacaacgtt
    13501 tggtaaaagg cttagccgat attttcgtgc cgatcattcc ggctattgtg gctggcggtt
    13561 tgttaatggg tattcactca atgctaacgg cagtcggttt cttctgggaa ggtgaatctg
    13621 ttgttagccg ttatccgaat atttccgatt tagttgattt catcaacact attgctaacg
    13681 caccatttgt attcttacct gtattactcg gtttctcagc tacacgcaaa tttggcggta
    13741 atccattctt aggtgcggca cttggtatgt tattggttca cccggcactt gcagacggtt
    13801 ggaactatgc caaaacctta atggaaggta acatcaaata ctggaatatt ttcggcttag
    13861 aaattgaaaa agtgggttat caaggcacgg ttattccaac tatcgtttcg gcttgggtgt
    13921 tagcaaccct tgagaaaact ttccgtaaat ttgtgccaag ctatttagat aacttggtga
    13981 cgccaatgat gtcattattt atcgccggta ttttagcttt cactgtaatc ggaccattgg
    14041 gtcgtgaagc aggttcagcg atttcagcag gcttaacgtg gttatatgac agcttaggct
    14101 ttatcggtgg agcaattttc ggtacattct acgctccaat cgtgattacc ggtatgcacc
    14161 aaacctttat tgcggttgaa acccaattat tagctgaagt agcacaaacc ggcggtacat
    14221 ttatcttccc gattgcagca atgtctaaca ttgcacaagg tgcagcctgt ttaggtgtag
    14281 cttttgcaat gaaagatgcc aaagtgcgtg gtttagccgt gccgtcaggt atttcagccc
    14341 tgttaggtat caccgaaccg gcaatgttcg gagtaaattt acgctaccgc caagccttta
    14401 ttgcggcgat gatcggttca ggtttagcca gtgcatttat cgctttcttt aatgtgaaag
    14461 caattgcctt aggtgcagcc ggtttcttag gtattccgtc gattaaacct gacagccttg
    14521 caatgtattc tgtcggtatg gtaatttcat ttgtagttgc atttaccatt tcagccattt
    14581 gggtgaaaaa agcacaagcg aaataatcaa aaatagatca caaaaaaggg attaagtgtt
    14641 agcttaatcc cttttgtttt taaacgcctt agcttaacaa tgagtttcgc aagcggtcat
    14701 ttttcgctaa aaatttgcaa attagctgtt gtgcataatt cgagaaatac gcccttcggc
    14761 aatctcttca tcacgaattg tcagcacttc acagccgtct ttggtaacta caatttgatg
    14821 ctcatattgt gcggagtggc tgcggtcttt ggttttcact gtccaaccgt cacccattaa
    14881 gcggacttct tttttgccgg cattcaccat tggctcaatc gtaaacacca ttccttcttt
    14941 taacactaca ccgccatcat cggtagcata atgcactact tgtgggtcgc aatggaattc
    15001 agtaccaata ccgtgtccgc aatattctcg tactaccgaa aagccttctt tttccacata
    15061 tttttgaatc gctgtaccaa tttcttttaa acgcaccccc gctttcacgg tgcgaatccc
    15121 gacataaagg gactctagcg taaccgccat taagcgttta tcccgcacac ttggctcacc
    15181 gactacatac atcatcgagt tatcgccata gtagccatct ttgataaccg tcacatcgat
    15241 gtttaaaata tcgccttttt tcagcttttt gtcatcactt ggaataccgt gacaaacgac
    15301 ctcatttaac gaaatgcaca ccgattttgg gaagccgtga tagcccaaac tggctgaagt
    15361 tgccccattt gccaaaatgt attcgtgaca aattttatct aattcaccag ttgttacgcc
    15421 ctctttcaca taaggggcga tcattactaa aacatctgaa gccagtttac aggctactcg
    15481 tagtttttga atttcttctt cgtttcttaa tggaatatta ttcattacat tgctctctgc
    15541 tatatctgcc ataaaaagta aggggattat accttttttc agcacaatcc ccaaacctca
    15601 gtttagattt ctactttaaa tcaagtttct tcataatgaa atttaatatc acaccat
//
LOCUS       DANKBV010000049        15625 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.049, whole genome shotgun sequence.
ACCESSION   DANKBV010000049 DANKBV010000000
VERSION     DANKBV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15625
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.049"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(115..1830)
                     /gene="proS"
                     /locus_tag="PWY43_001253"
     CDS             complement(115..1830)
                     /gene="proS"
                     /locus_tag="PWY43_001253"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL2136136.1"
                     /translation="MRTSQYLFSTLKEMPNDAQVVSHQLMLRAGMIRPMASGLYNWLP
                     TGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELWVESGRWNDYGPELLRFKDRGER
                     DFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
                     YSFHTTQESLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGE
                     DDVVFSTESDFAANIELAEAVAVGERQAPTAEMQLVDTPNAKTINELVENHGLAIEKT
                     VKTLVVKGATEEQPLIALVIRGDHELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVG
                     SLGVVNMPIPVIIDRSVALMSDFGCGANIDGKHYFNVNWERDVAVPEIADLRNVVEGD
                     PSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVV
                     AAAIEQHYDDRGIIWPCDEMAPFTVAIVPMNMHKSEKVQAFAEELYANLKAQGVEVIF
                     DDRKERPGVMFADMELIGVPHMVVIGEKNLENGEIEYKNRRSGEKQMIAKESLLAFLK
                     ANVKA"
     gene            complement(1994..2176)
                     /locus_tag="PWY43_001254"
     CDS             complement(1994..2176)
                     /locus_tag="PWY43_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136137.1"
                     /translation="MEQKLNNVFGGIMFKEKDYDEYLEAKVKKGLAEIEKGQIFTLEQ
                     ANAEWQEIIEQIEREN"
     gene            complement(2229..4112)
                     /gene="htpG"
                     /locus_tag="PWY43_001255"
     CDS             complement(2229..4112)
                     /gene="htpG"
                     /locus_tag="PWY43_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDL2136138.1"
                     /translation="MANNQETRGFQSEVKQLLQLMIHSLYSNKEIFLRELISNASDAA
                     DKLRFKALSEPALYEGDGELRVRVSVDETLGTITIADNGIGMTREQVIDHLGTIAKSG
                     TKEFLNALGTEQAKDSQLIGQFGVGFYSSFIVADKVTVKTRAAGVPAEQGVIWESAGE
                     GDYTVADLEKKERGTEIILHLREDEKEFLNEWRLREIIGKYSDHIGLAVEIQTNEYDD
                     EGKASGQKWEKINKAQALWTRSKNEISDEEYTEFYKHLSHDFSDPLIWSHNKVEGKQE
                     YTSLLYVPSKAPWDLFQREQKHGLKLYVQRVFIMDDAEVFMPNYLRFMCGLLDTNDLP
                     LNVSREILQENKVTQSLRSALTKRALQLLEKLAKDDAEKYAQFWQAFGVVLKEGVGED
                     FANKQQVAGLLRFASTQRDDSEQAVSFADYIARMKEGQKAIYFLTADSYQAAKNSPHL
                     ELFHKKGIEVLLLSDRIDEWLVSHLTEFDGKPLQSITKSDLDLGELADKQEEESQKAQ
                     QAEFGSFLERAKGYFGERVKNVVLTHRLTDTPAVVSTDSDEMTTQMAKLFAAMGQQAP
                     EVKYTFELNPDHPMVKKVADLADEAEFADWIELLFEQALLAERGTLENPTAFIKRVNK
                     LLG"
     gene            4276..4563
                     /locus_tag="PWY43_001256"
     CDS             4276..4563
                     /locus_tag="PWY43_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL2136139.1"
                     /translation="MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDY
                     QQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVEIRKSNKPRPVDFGDE"
     gene            4563..4991
                     /locus_tag="PWY43_001257"
     CDS             4563..4991
                     /locus_tag="PWY43_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL2136140.1"
                     /translation="MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCIT
                     LSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQTKAFVVNNDIALLASEYHIPNKM
                     DLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA"
     gene            4995..7076
                     /gene="recG"
                     /locus_tag="PWY43_001258"
     CDS             4995..7076
                     /gene="recG"
                     /locus_tag="PWY43_001258"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL2136141.1"
                     /translation="MSEQLLDGVPLTALSGVGAAISEKLSRIGINNVQDLLFHLPMRY
                     EDRTRITPIADVRPESFATIEGYVQLTEVQFGKRPILSVTLSDSTSKIMLKFFNFNAG
                     MKNSLATGVRVKAFGEIKRGRFMAEIHHPEYQIIRDNQPLVLAETLTPIYSTTEGLKQ
                     ASLRKLTEQALALLEKVKVAELLPDEYNPHKYSLKEALQLLHRPPPTISAEVLEKGDH
                     PAQKRLIFEELLAHNLAMQQLRLGVKQQYAEALTYQTDIKQRFLDSLPFKPTHAQSRV
                     TADIEQDLAKPHPMMRLVQGDVGSGKTLVAALAALLAIDNGKQVALMAPTEILAEQHA
                     NNFANWLNPFGIEVGWLAGKVKGKARTAQLEAIKNGDVQMIIGTHALFQDNVEFHHLA
                     LVIIDEQHRFGVHQRLTLREKGAKENNYPHQLIMTATPIPRTLAMTVYADLDTSIIDE
                     LPPGRTPITTVVISEDRRAEIVQRVYQACKQEHRQAYWVCTLIDESEVLEAQAAAAIA
                     EDLQRALPDLRIGLVHGRLKPQEKQAIMAEFKAANLDLLVATTVIEVGVDVPNASLMI
                     IENSERLGLAQLHQLRGRVGRGATASHCVLMYKPPLGKISSKRLQVMRDSQDGFYIAE
                     KDLEIRGTGEILGTKQTGMAEFKVADLMRDRKMIPLVQRYAKQIISENQPLAEALIKR
                     WLDNKAEYTNA"
     gene            complement(7098..8030)
                     /locus_tag="PWY43_001259"
     CDS             complement(7098..8030)
                     /locus_tag="PWY43_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDL2136142.1"
                     /translation="MAQKIILDCDPGHDDAIAMMLAWGNPNIDLLAVTTVVGNQTLEK
                     VSRNALAVAEIAHICNVPFAKGCARPLVREVENAPDIHGDSGMDGLVLPESTRQFEKK
                     HAAQLIIDLIMSHPEKSITLVPTGGLTNIALAARLEPRIIERVKEVVLMGGGYHTGNW
                     SAVAEFNIKIDPEAAHIVFNAGWKVTMVGLDLTHQALATPDVVERFKALNSKSGQFVV
                     ELLAFFAKMYKQAQGFDAPPVHDACAVAYVIDPSLIEVQQVPVNIELTGTHTLGMTVA
                     DFRYPPKQCNTWVAKKLDKSRFWDLVIESVKNLP"
     gene            complement(8083..9735)
                     /locus_tag="PWY43_001260"
     CDS             complement(8083..9735)
                     /locus_tag="PWY43_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2136143.1"
                     /translation="MTIFQVAQNWLAQDPDAETRAELEQLIQAAESDEKAKAELTARF
                     DGRLQFGTAGLRGRLQAGSMGMNRVLVAQAAGGLAGFIKGYDKEPSIVIGYDGRKNSD
                     VFARDTAEIMAAAGIKTYLLPRKLPTPVLAYAIQYFDTTAGVMVTASHNPPEDNGYKV
                     YLGKANGGGQIVSPADKEIAALIDKVAAGDIRDLPRSQDFTVLDDEVVNAYIEKTASL
                     AKWPKAEISYVYTAMHGVGYEVLSKTLEKAGLPQPYLVSEQIQPDGSFPTVNFPNPEE
                     KGALDLAIKLAKEKNAEFIIANDPDADRLAVAVPDAQGNWKPLHGNVIGCFLGWYLAK
                     QFHAQGKQGVLACSLVSSPALAEIAKKYGLSSEETLTGFKYIGKVENLLFGFEEALGY
                     LVDPDKVRDKDGISAAIMFLDLVCSLKQEGKTLADYTTEFVQEFGAYVSGQISIRVCD
                     LAEIGKLMTALRNNPPSEIGGFKVAEFIDHTKTTRQNDILVFVLENGSRLIARPSGTE
                     PKIKFYLDARGTDAENAEQVLAQFDESVRSLLRQEEYGKQDC"
     gene            complement(9886..10947)
                     /locus_tag="PWY43_001261"
     CDS             complement(9886..10947)
                     /locus_tag="PWY43_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2136144.1"
                     /translation="MAIYAIWNNKGGVGKSYLTFQIACEYAKQYPAKKVLVVDLCPQA
                     NSSSMLLGGLEKGEQELAKIHSQVNNNTISGYISERLVSPYRSINSATKYVTQVSTIN
                     EYVPKNVYLVVGDEELEIQSSGILHASQAVHVPDSWRLVHLWVRDLVSDIQNAWDNDD
                     NVVFIDCNPSFTIYTELALAASDRLIIPFSADGSSKRAVKSVLSLVYGVKRHSGDVSS
                     HFYTESQKSKLALPQIYMYVGNRLTINAKASATAFKTVVEEIGDEIYNIWKTNANLFC
                     IHLAGVGTPTNKSSFRKMFQYEVRDANTASVVSGALGIPISRLSSGKRDVLGKNVMVN
                     QSQLDAQVPNIQNLVQCIE"
     gene            complement(11028..12260)
                     /locus_tag="PWY43_001262"
     CDS             complement(11028..12260)
                     /locus_tag="PWY43_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL2136145.1"
                     /translation="MEHYLDELIINSQRILLLQGPIGSFFTEFARWLESEQKEVFKLN
                     FNGGDEYFYPNNSPNTFPYYDSLDNFEAYLIQFCQTHQIDNILCFGDNRYYHKVAKNV
                     CLKLKLVFWAFEEGYFRPEYITLEKWGVNAFSPIPKNHTFFLPYVELPVVSEPEKVAK
                     GFRCMAGKAIPYYWNAYKKRKAYAKYKHHRFLNLKYYLNLWTRSGIKRICYWLHDKGF
                     AKRVEKGEFGEFFIVPLQVYDDTQVRIHSEQKSVERFLREVLNSFAKFAPAHLNLIIK
                     HHPMDRGFIDYKMVIYEFLEQYPQLNGRVFYIHDVPLPIFLRYGKGMVTLNSTSGLSA
                     LLHNMPVKTLGRANYDFEGLTDKQPLDTFWANPQPPNSEVFKGYRQFHLHKTHINGNF
                     YHKSILRYPYNWENEAHR"
     gene            complement(12283..14373)
                     /locus_tag="PWY43_001263"
     CDS             complement(12283..14373)
                     /locus_tag="PWY43_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDL2136146.1"
                     /translation="MLISLIFSNGICRIPNLEHFLPDCRIIRIPSWRKKLGNKAKVDQ
                     IIGWGLRPSAVGARAYAQKHQLPYIALEDGFLRSLGLGVKGYPPFSLVYDDVGIYYDT
                     TCPSRLEKFLLEQPLQDQEQIKLAEKALSQILTYQLSKYNHALDFSEIPSIKEKIVLV
                     IDQTFGDMAVKYGQASEVDFNKMLETAISENPEAQIWVKTHPDVLSGKKKGYLTDLPQ
                     YADRVRIITEDVNPASLIKWCEKVYCVTSQMGFEALLLGKKVVTFGVSWFAGWGVTDD
                     RHNSVQLLIQQKRRQARSLLQLFYAAYFDYSRYINPNTGEKGSIFDVIEYLHKATILN
                     RRLSGNVYCIGMSLWKQAVIKPFFNLPQCRLHFVRSLNSLKKRPLAENAKILVWSNGK
                     PEIIQFAKENHIPLLRMEDGFIRSVGLGANLVAPLSLVVDDLGIYFNAENPSRLEQIL
                     LEAEDFSENDLSYAEALRQKLVATHIGKYNVGSGEVQVNAQGKKIILVPGQVEDDASI
                     KSGSPQIKRNLDLLKKVRELNPSAYIIYKPHPDVVSGNRVGQIPTAETRRFADQMVDN
                     ANILDCILIADEVHTMTSLAGFEALLRGKKVHCYGLPFYAGWGLTEDRLKSARRGKSL
                     TLSQLIAATLIYYPLYVDPISRQFIDANQAIHILSQQKRNLTNQRIKRSWVSKQYGKL
                     QHLYRAFKYASHTK"
     gene            complement(14612..>15625)
                     /locus_tag="PWY43_001264"
     CDS             complement(14612..>15625)
                     /locus_tag="PWY43_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136147.1"
                     /translation="AKFKSIVLKHRLHRNLTIKEITEIESVAVKRNNMNWLRSVSYIQ
                     YSYGMYDECFKNLKRLEESDSIIYSLDYMTMAECCKRDNEYEKYNLKELDNTFYYKYE
                     NNKSSTLLVTLSATNNFALQKYEYSSDKLFLTDNSHSYYMLSYNKIGKLIEKLCNDNN
                     YRKICIVGLSKGGTGALYLFDYIQNHIPSVEIRCIAFSPQVNIYPFNRNLNIPSYRKL
                     QEYIDCNTLIEHKVQNVKPIKNIILNSSQNKVFVFYGRNFTMDLIEARELKHFELVEL
                     PYSGHATVIPLTVPEGKTKEWLSNLTVDPDLAALGGITDLIDEIWEIYQNSNMRLYKF
                     LDS"
BASE COUNT         4534 a         3503 c         2903 g         4685 t
ORIGIN      
        1 cacgcattct atttcctata ttctaaaaat aatagagtat tttatacttt agttaaatac
       61 tcgctgcttt atattaaagg gcgtatttta tacgcccaat tttgttattt ataattaagc
      121 ctttacattc gcttttaaga acgcaagtaa gctctctttg gcgatcatct gtttttcacc
      181 gctgcggcgg tttttgtatt cgatttcgcc gttctcgagg tttttctcac caataaccac
      241 catatgcggc acaccgataa gctccatatc cgcgaacatc acgcccgggc gttctttacg
      301 atcatcaaaa atcacctcaa cgccttgagc ttttaagtta gcgtataact cttcagcgaa
      361 tgcctgcact ttttccgatt tgtgcatatt catcggcaca atcgccaccg taaatggcgc
      421 catttcatca caaggccaga taataccacg atcatcataa tgttgttcaa tcgcagctgc
      481 caccacacgg gtaacgccga taccgtaaca acccattgtc atcactagcg gtttaccgtc
      541 ttcaccttgc acagtggctt tcattgcttc tgagtatttt ttgcctaatt ggaaaatatg
      601 ccctacttca atacctcgtt tgatttgaag tgtgccttta ccgtccgggc ttgggtcgcc
      661 ttccaccaca ttgcgtaaat ctgcaatttc cggcacagca acatcacgct cccagttcac
      721 gttaaagtag tgtttgccat caatattcgc accgcagcca aaatctgaca tcaacgccac
      781 tgagcggtca ataatcaccg gaatcggcat attcaccaca cctaacgagc caacaccggc
      841 accgattttg gctttaattt ccgcttcatc ggcaaactca agcggatcag ccaccaacgg
      901 gtgtttttga gctttaattt cgtttaactc gtggtcgcca cgaatcacta acgcaattaa
      961 cggttgctct tcagttgccc ctttcaccac taaggttttc accgtttttt caatggctaa
     1021 accgtgattt tctactaatt cattgatggt tttcgcattt ggcgtatcca ctaattgcat
     1081 ttctgccgtt ggtgcttggc gttcacccac cgcaaccgct tcggctaatt caatattggc
     1141 agcaaaatct gattcggttg aaaataccac atcatcttca ccgcttgagg ctaacacttg
     1201 gaactcgtga gaggctgaac cgccgataga gccggtatct gcctgcaccg cacggaaatc
     1261 taagcccaaa cgggtgaaaa tattgctgta aacctgatac atcacatcat aggtttcttg
     1321 caggctctct tgtgtggtgt ggaaagaata ggcatctttc atcacaaatt cacgcgaacg
     1381 cattaccccg aaacgagggc ggacttcatc acggaatttc gtttgaattt ggtataaatt
     1441 cagcggaagt tgtttgtacg aagaaacttc acggcgaacc aagtcggtaa tcacttcttc
     1501 gtgagtcggg ccaagcacga aatcacgctc gccacgatct ttaaaacgta ataattccgg
     1561 gccgtaatca ttccaacgac cggattccac ccataattcc gccggctgaa ccaccggcat
     1621 taatacctcg atagccccgc ctttgttcat ctcttcacga ataatatttt ccactttttt
     1681 caacactttg atcccggtcg gcaaccagtt gtataaacct gacgccatcg ggcgaatcat
     1741 tccggcacgc aacattaatt gatggctaac tacttgtgca tcattcggca tttctttaag
     1801 ggttgaaaat aaatattgac ttgtacgcat tgctattcct attcttttta aactttgaaa
     1861 cctgaaaaaa tctgcttatt ttaaaacaaa gcggttaaat tttcaggaaa ttttgcaaaa
     1921 tcctataaaa aaataaccgc ttgtaagcgg ttattttgtt ttaactaagg gtattttctt
     1981 aaactgtgta tgcttaattt tctcgttcaa tttgctcaat aatctcttgc cattcggcat
     2041 ttgcttgttc aagagtaaaa atctgacctt tttcgatttc tgccagacct ttttttactt
     2101 tagcctctaa atattcatca taatcttttt ctttaaacat tattcccccg aatacattgt
     2161 ttaatttttg ttccattata tattgaatat ggaaaaacgg aaacctagat ttccgttttt
     2221 agtaatggct agcccaacaa tttatttacc cgtttaataa aggctgtcgg gttttccaac
     2281 gtgcctcgtt cagcaagcag agcttgttca aacagcagtt caatccaatc ggcgaactcg
     2341 gcttcatcgg caagatcagc tacttttttc accattgggt gatctgggtt cagctcaaaa
     2401 gtatatttca cttctggtgc ttgttgcccc attgctgcaa acagttttgc catttgggtg
     2461 gtcatttcat cgctgtcagt ggaaaccact gccggtgtgt cggttaagcg gtgagtcagc
     2521 accacgtttt tgacccgttc accaaagtag cctttcgccc gttctaagaa cgaaccaaac
     2581 tccgcctgtt gggctttttg gctctcttct tcctgcttgt cggcaagctc gcctaagtct
     2641 aagtccgact tggtaatgct ttgtagcggt ttgccgtcaa actcggtgag gtggctaacc
     2701 aaccattcat caatgcggtc ggaaagcagt agcacctcaa tgcctttttt gtggaacagc
     2761 tctaagtgcg ggctgttttt agccgcttgg tagctgtctg cggttaagaa gtagatcgct
     2821 ttctgcccct ctttcattct tgcaatataa tcggcaaaac tgaccgcttg ttcgctgtca
     2881 tcacgctggg tggaggcaaa acggagtaag cctgcaacct gttgtttgtt ggcaaagtct
     2941 tcgcccacgc cctctttcag caccacgccg aacgcttgcc agaattgggc atatttttcg
     3001 gcatcgtctt tcgccaattt ttccagcagt tgtaacgcac gcttggttaa ggcagaacgg
     3061 agcgattggg taactttatt ctcttgcaga atttcacggc tgacgttgag cggcaggtcg
     3121 ttggtgtcga gcaagccaca cataaaacgc aagtagttcg gcataaagac ttccgcatcg
     3181 tccataataa atacacgctg aacgtagagt ttcaagccgt gtttttgctc gcgttggaac
     3241 agatcccacg gggctttgct cggcacatac agcaagctgg tgtattcttg cttaccttcg
     3301 actttattgt gcgaccaaat cagcgggtcg gaaaaatcgt ggctgaggtg tttgtaaaat
     3361 tcggtgtact cctcgtcact aatctcattt tttgagcgtg tccaaagcgc ttgagctttg
     3421 ttaatttttt cccatttttg accgcttgct ttgccctcgt catcgtattc attggtctga
     3481 atttccaccg ccaggccgat atggtcggag tatttgccga taatctcacg caaacgccat
     3541 tcgtttaaaa actctttttc gtcctcacgc aagtgaagaa tgatttccgt gccacgctct
     3601 tttttctcca gatcggcaac ggtgtaatcg ccctcaccgg cagattccca aatcacgcct
     3661 tgctcggcag gcactccggc ggcacgggtt ttgactgtca ctttatccgc cacgatgaaa
     3721 gaagagtaaa agcccacgcc gaactgcccg ataagctggc tgtctttggc ttgctcagtg
     3781 ccgagtgcgt tcaaaaattc tttggtacca gattttgcaa tcgtaccgag atggtcgatc
     3841 acctgctcac gggtcatacc gataccgtta tcggcaatcg taatcgtacc gagtgtttca
     3901 tcaacagaga cacgcacacg cagttcgcca tcaccttcat acaaagccgg ctcggaaagg
     3961 gctttaaacc gcaatttatc ggcggcgtca gaggcgttgg aaataagctc acgcaggaaa
     4021 atttctttgt tggagtaaag cgagtggatc atcagttgca ataactgttt cacttccgac
     4081 tggaagccac gggtttcttg attatttgcc attgtgtatt cctttattca ttaaaaaaca
     4141 gggaatagat ggggcaagtg gtcagatttt caagattttt tgcaaaatat ctgttgccaa
     4201 atagcgaatt aattgaaaga tattactaca tttaataaaa tacactcatc tttttactac
     4261 attttggaga tcgatatggc aataaccatt acttccagag aatttaatca aaatgccagc
     4321 aaagtgttaa aaatggcaga aaaagagccg gtttttatta ccaaatgggg caaaatcgtc
     4381 aatgtggtgt ctagttatat ggattatcag cagaaaaagc ctagcgagcc aagctttgaa
     4441 gagctattcg cctcttccga aaatgaggat gtgagcgatg aattttggca agagtttgaa
     4501 caaacattgg ttgaaatccg caaaagcaat aaaccaagac ctgttgattt tggagatgag
     4561 taatgtatcg ctaccttttt gataccaata tagtttccga gctttataag ttaagaaatg
     4621 acaagatgga tgtcaatgtt cgccagtggc tgagaggggt taatcctagc cagaccaata
     4681 tcagctgtat tacgttatct gaaatcaaaa caggtatttt gctgaaagcc cgcaaagaca
     4741 aagaacaatc agaccgatta aatgattggt ttgaaaataa tgtattaaag gcttatcaaa
     4801 caaaagcgtt tgtggtgaat aacgacattg ctctattggc atcagagtac catattccca
     4861 ataaaatgga tttaaatgat gcttatattg cggcaaccgc caaataccac aatctgattt
     4921 tagtcacccg caacaccaaa gattttatcc gttgtgatgt gcgtttattt aacccatttg
     4981 aaaccgcata aataatgagt gaacaattat tagacggcgt gccattaacc gccctttccg
     5041 gcgtaggagc ggcaatttcc gaaaagttaa gtcgaatcgg cattaataat gtgcaggatt
     5101 tactgtttca tctgccaatg cggtatgaag atcgcacccg tattaccccg attgctgatg
     5161 ttcgcccgga aagctttgcc accattgaag gctatgtaca acttaccgaa gtccagtttg
     5221 gtaaacgccc gattttatcg gttacactct ccgacagcac cagcaaaatt atgcttaaat
     5281 tcttcaattt caatgctgga atgaaaaata gccttgccac cggcgttcgg gtaaaagcct
     5341 ttggggaaat taagcgtggg cgatttatgg cggaaattca ccaccctgaa tatcaaatta
     5401 ttcgggataa tcaaccgctt gtactggctg aaaccctgac cccgatttac tccaccactg
     5461 aggggttaaa acaagcctcg ctgcggaaat taaccgagca agccctcgct ttattagaga
     5521 aagtgaaagt cgccgagctg ttgcctgatg aatacaatcc gcacaaatac agcctaaaag
     5581 aagcgttgca gttattgcac cgcccaccgc cgacgatttc tgccgaagtc ttggaaaaag
     5641 gtgaccaccc tgctcaaaaa cggctgattt ttgaagaact gctggcacac aatttagcga
     5701 tgcagcaact ccgtttaggg gtaaaacagc aatatgcaga ggcacttact taccaaaccg
     5761 atataaaaca gcgttttctc gacagtttgc cttttaagcc tactcacgca caaagccgag
     5821 taaccgcaga tattgagcaa gatcttgcca aaccacaccc gatgatgcga ctggtacaag
     5881 gcgatgttgg ttcaggtaaa accttagtag ccgccttggc agcactatta gcgattgata
     5941 acggcaagca ggtggcatta atggcaccga ccgaaattct tgccgaacag cacgccaata
     6001 attttgccaa ctggcttaat ccctttggga ttgaggttgg ctggcttgcc ggtaaagtga
     6061 aaggcaaggc gagaaccgca caacttgagg caatcaaaaa cggtgatgtg cagatgatta
     6121 tcggcactca cgccctgttc caagacaatg tggaatttca ccatttagcg ttggtgatta
     6181 ttgatgaaca acaccgtttt ggcgtacacc aacggctgac cttgcgggaa aaaggagcga
     6241 aagaaaataa ctatccgcat cagctcatta tgacggcgac ccccattccg agaacgctgg
     6301 caatgacggt ctatgctgac ctcgacacct caatcattga cgaactgcca ccgggtagaa
     6361 cgccaatcac gactgtggtg atttctgaag atcggcgagc agaaatcgtc caacgggtgt
     6421 accaagcctg taagcaagaa caccgccaag cctattgggt ctgcaccctg attgatgaaa
     6481 gcgaagtgtt ggaggctcaa gccgcggcag cgattgccga agatttacag cgagccttgc
     6541 cggatttacg catcgggtta gtacacggac ggctgaaacc acaggaaaaa caagcgatta
     6601 tggcggaatt taaggcagcg aatttagatt tgctggttgc gaccaccgta attgaagtgg
     6661 gagtggacgt gccgaatgct agcctaatga ttatcgaaaa ttccgaacga ctcgggctgg
     6721 ctcaactcca ccagttgcgt ggacgtgttg ggcgaggggc aaccgcctcg cattgcgtct
     6781 taatgtacaa accgccgctc gggaaaattt ccagcaagcg gttacaagtg atgcgtgata
     6841 gccaagacgg tttctacatt gccgaaaaag atttggagat tcgaggcaca ggtgaaattc
     6901 tcggcacaaa acagaccgga atggcagaat ttaaggtggc ggatttaatg cgagaccgca
     6961 aaatgattcc gctggtgcaa cgttacgcca agcaaattat tagcgaaaat caaccgcttg
     7021 cagaggcgtt aattaagcgt tggttagaca acaaggcgga atataccaac gcttaaccgg
     7081 caagcgggtt atttagatta cggcaggttt ttcaccgatt caattaccaa atcccaaaag
     7141 cgggatttgt ctaatttttt tgctacccaa gtattacact gttttggcgg gtagcggaaa
     7201 tccgccaccg tcatacccag tgtatgcgtg cctgttagct caatattgac cggcacttgc
     7261 tgaacttcaa tcaggcttgg gtcaatcaca tacgccaccg cacaggcatc gtgaaccggt
     7321 ggagcatcaa agccttgagc ctgtttatac attttggcga agaacgccag caattccacc
     7381 acaaactgcc ccgatttgct gtttaatgcc ttaaagcgtt caaccacatc gggggtcgca
     7441 agtgcttggt gagttaagtc taagccgacc atcgtcacct tccaaccggc attaaacaca
     7501 atatgagcag cttccgggtc aattttaata ttaaattctg ccaccgcact ccagttgccg
     7561 gtgtgataac ctccgcccat cagcaccact tctttcactc tttcaataat acggggctca
     7621 aggcgggcag ctagagcaat attggttagc ccaccggtcg gcactaaggt aatgcttttc
     7681 tccgggtgcg acattatcaa atcaataatt aattgagcgg cgtgtttttt ctcaaattgt
     7741 cgggtagatt cagggagtac aagcccatcc attcccgaat caccgtgaat atccggggca
     7801 ttttccactt cacgcaccag cggtctggca caacctttgg caaaaggaac gttgcaaata
     7861 tgagcaattt ccgcaaccgc caacgcatta cgcgatactt tttctaaggt ttgattgcct
     7921 accaccgtag tcactgccaa caaatcaata ttcggattcc cccaagcgag catcatcgca
     7981 atcgcatcgt catgccccgg atcacaatct aaaataattt tttgagccat tattgtctcc
     8041 tttaaaaaag ggagagccta agctccccct caattaatgc cattagcaat cttgtttgcc
     8101 gtattcttct tggcgaagca gcgaacgcac actttcgtca aactgagcca gcacttgctc
     8161 tgcgttttca gcgtctgtgc cacgagcatc taaatagaat ttaattttcg gctcagtacc
     8221 tgaagggcga gcaatcaaac ggctgccatt ttccaacaca aacactaaaa tgtcattttg
     8281 gcgtgtggtt ttggtatggt caataaactc agccacttta aagccaccaa tttcgcttgg
     8341 aggattgttg cgtaacgccg tcattaattt gccgatttcg gctaaatcac aaactctgat
     8401 cgaaatttga ccgcttacat aagccccgaa ctcttgtaca aactcggtgg tgtaatccgc
     8461 aagggtttta ccttcttgtt tgaggctgca cactaaatct aagaacataa tggcagcgga
     8521 aatgccgtct ttatcacgca ctttgtctgg gtcaaccaaa taacctaagg cttcttcaaa
     8581 gccaaataat aggttttcaa cttttccgat atatttaaag ccggttaagg tttcttccga
     8641 gcttaaacca tattttttcg caatttcagc taacgccgga gaggatacta acgagcaagc
     8701 caacacgcct tgtttacctt gtgcgtggaa ttgtttggct aaataccagc ctaagaaaca
     8761 gccgattaca ttgccatgca gtggtttcca gttgccttga gcatcaggca cggctaccgc
     8821 taaacggtca gcatccgggt cgttggcgat aataaattcg gcatttttct ctttcgccaa
     8881 tttaatcgct aaatctaacg cccctttctc ttccgggttc gggaaattaa ccgttgggaa
     8941 cgaaccgtcc ggctgaatct gctcactaac taaatacggt tgcggtaagc cggctttttc
     9001 taatgttttg cttaacactt catagcccac accgtgcatt gcagtataaa cgtagcttat
     9061 ttcggctttt ggccatttgg ctagtgaggc ggttttctca atataagcat ttaccacttc
     9121 atcgtctaac accgtgaaat cttggctacg aggtaaatca cgaatatcgc ctgctgctac
     9181 tttatcaatc agagcagcaa tttctttatc tgccggcgaa acaatttgtc cgccaccgtt
     9241 agctttacct aaataaactt tatagccgtt atcttccggt gggttgtggc tggcagttac
     9301 catcacaccg gctgtggtat caaaatattg aatcgcatag gcaagcactg gtgttggcaa
     9361 tttgcgagga agcaggtacg ttttaatgcc tgccgctgcc ataatttcag cggtgtcacg
     9421 ggcaaacaca tcggaatttt tacgaccgtc ataaccaata acaattgacg gctctttatc
     9481 ataaccttta ataaacccag ctaaaccgcc ggcagcttgt gccaccaaaa cacggttcat
     9541 acccattgaa cccgcttgta agcgaccacg taaacccgcc gtaccaaact gtaaacgtcc
     9601 gtcaaaacgg gctgttaatt ctgcttttgc tttctcgtcc gattctgccg cttgaattaa
     9661 ctgctctaat tccgcacggg tctctgcatc cgggtcttgg gctaaccaat tttgtgcaac
     9721 ttggaaaata gtcatttttt actcctatgt aatttaaaaa ctctgtttag attagcccaa
     9781 agtcctttta tttaaaagga gatatttctt ttttgtgatc gagctatcaa ataaaaagat
     9841 ttgtcagaaa taaaaaatag cgtacttaat atacgctatt tttctttatt ctatacactg
     9901 aacaagattt tgaatgttag gaacttgagc atctaattgt gactgattca ccattacatt
     9961 tttacctaaa acatctcttt ttcctgatga taagcgacta atcggaatac ctaaagcacc
    10021 tgaaacaaca gatgctgtat ttgcatctct cacctcatac tgaaacattt ttctgaagct
    10081 tgatttattt gtaggagtac caacacctgc taggtgaata cagaacaaat tagcattagt
    10141 tttccatata ttatagattt catcacctat ttcctctaca actgttttaa atgcggttgc
    10201 tgatgctttg gcatttatcg ttaatctatt acctacatac atataaattt gaggtaatgc
    10261 taacttactc ttttgagatt ctgtataaaa atgagagctg acatccccag agtgcctttt
    10321 aaccccatat actagagata atacagactt aacagctcgt tttgatgaac catcagcgga
    10381 aaaagggatg atcaatctat ctgaagcagc caacgctaat tcagtataaa tagtgaaact
    10441 tggattgcaa tcaataaata caacgttatc atcattatcc caagcgttct gtatatcaga
    10501 aactaaatca cgcacccaaa gatgcactag ccgccaagaa tcaggaacat gaacagcttg
    10561 ggaagcatgt aaaataccag atgattggat ttctaattcc tcatcaccaa caacaagata
    10621 aacatttttt ggtacatatt catttatagt gctaacttga gttacatatt tcgttgcact
    10681 attaatagaa cggtaaggac ttactaatct ttctgaaata tatcctgaaa tagtattatt
    10741 gtttacttga gaatgaattt ttgctaactc ttgctcacct ttttcaaggc cgcctaatag
    10801 catagatgat gaatttgctt gaggacataa atcaacaacg agaacttttt ttgctgggta
    10861 ttgttttgca tactcacaag cgatttgaaa agttaaataa cttttaccaa ccccaccttt
    10921 attattccat attgcataaa tagccataat gccccctctt ttaacaatag gtgtaataat
    10981 aacacaataa tttagctata agatattact tattttaata ttttccatca cctgtgtgct
    11041 tcattttccc aattataagg ataacgcaaa atcgatttat ggtaaaaatt accgttaata
    11101 tgcgttttat gtaaatgaaa ttgtctgtag cctttaaata cttccgaatt aggtggctga
    11161 ggattagccc aaaaagtatc taatggctgt ttatcggtta atccctcaaa atcataattg
    11221 gctctaccta aggttttgac cggcatatta tggagcaatg cagaaagccc actggttgaa
    11281 tttaaagtca ccattccttt cccataacgt aaaaagatag gtaacggcac atcgtgaata
    11341 taaaaaactc taccgtttaa ttggggatat tgctctaaga attcataaat caccatttta
    11401 taatctataa atcctctatc catcgggtga tgcttaataa ttaaattgag atgtgccggt
    11461 gcaaatttgg caaaggaatt cagcacttcc cttaaaaaac gctctacact tttttgttca
    11521 ctatgaatcc tcacttgagt gtcatcataa acttgtagag gcacaatgaa aaattcacca
    11581 aactctcctt tttctacccg cttggcaaat cctttatcat gtaaccaata acatattcgc
    11641 ttaattcccg accttgtcca taaattaagg tagtatttga gattcagaaa acgatgatgc
    11701 ttatattttg cgtatgcttt acgtttttta taggcattcc aatagtacgg gattgctttt
    11761 cccgccatac atctaaagcc tttagctact ttttctggtt cactgactac tggtaactcg
    11821 acataaggca aaaagaaagt gtgatttttc gggattggag aaaaagcatt tacaccccat
    11881 ttttccaacg taatatattc tggcctaaag tacccttctt caaacgccca aaagaccaac
    11941 tttaacttca agcaaacgtt tttagccact ttatgataat aacgattatc gccaaaacat
    12001 aagatattat ctatttgatg agtttggcaa aactgaatta aataggcttc aaaattatct
    12061 aagctatcat agtaaggaaa agtattcggg ctattatttg gatagaaata ttcatctccg
    12121 ccattaaaat ttaacttgaa gacctctttt tgctcacttt ctaaccaacg ggcaaactca
    12181 gtgaaaaaag agcctatcgg gccttgtaac aataaaattc tctggctatt gatgattagc
    12241 tcatctaaat aatgttccat cttaatttcc taccctctac gcttatttag tgtgggaagc
    12301 atatttaaac gcacgataaa gatgctgtaa ttttccatat tgtttactca cccaagaacg
    12361 tttaatcctt tgattggtta ggtttcgttt ctgttgtgac aatatatgaa tcgcttgatt
    12421 ggcatcaata aactgcctgc ttataggatc aacatacaga gggtaatata tcaatgttgc
    12481 ggcaattaac tgagataatg tcaaagattt acctctgcgt gccgacttca atctatcttc
    12541 cgtcaatccc caaccagcat aaaaaggtaa tccgtagcaa tgcacttttt tacctcttaa
    12601 taatgcttcg aaaccggcta aagatgtcat tgtatgcact tcatctgcaa ttaaaataca
    12661 atctaaaata ttggcattat ccaccatctg atccgcaaac ctgcgtgttt ctgccgtagg
    12721 aatctgaccg actcgattac cacttaccac atcagggtga ggcttataaa taatataggc
    12781 actagggttt aattccctca cttttttcag taaatccaga tttcttttaa tttgtggaga
    12841 accggacttt atcgaagcgt catcttcaac ctgccccggt actaaaatta tttttttgcc
    12901 ttgggcatta acctgcacct caccactacc gacattatat ttaccaatat gggtggccac
    12961 taatttttgt cttaatgcct cagcataaga caaatcattt tcagaaaaat cctccgcttc
    13021 caataaaatt tgttccaaac gggaaggatt ctctgcatta aaataaatac ctaaatcatc
    13081 tactaccaaa gaaagtggcg caactaaatt tgccccaagt ccaaccgaac ggataaaacc
    13141 atcttccatt ctcaacagtg gaatatgatt ttctttcgca aattgtataa tttcaggctt
    13201 accattactc cacactaaaa ttttagcatt ctctgcaagc ggtcgttttt tcaaagaatt
    13261 tagcgaacgc acaaaatgta aacggcattg aggcaaatta aaaaacggct taatgacagc
    13321 ctgtttccat aatgacatac ctatacaata tacatttccg cttaaacgcc ggttgagtat
    13381 tgtcgccttg tgcagatact caatcacatc aaaaatagag cctttttcac ccgtattcgg
    13441 attaatataa cgactgtaat caaaatatgc tgcataaaat agttgtaaca atgagcgtgc
    13501 ttgccgtctc ttctgttgga ttaataattg aactgagtta tgccgatcat ctgttacccc
    13561 ccaaccggca aaccacgaca caccaaaggt aaccactttt ttacctaaca ataatgcctc
    13621 aaagcccatt tgcgaagtta cgcaatagac tttctcacac catttaatca agcttgccgg
    13681 attaacatct tcagtaataa tacgaactct atcagcatat tgaggcaaat cggtcaaata
    13741 gcctttcttc ttgccactta atacatcggg gtgagttttc acccaaatct gagcctctgg
    13801 attctcactg attgccgttt ccaacatttt attgaaatcc acttcactcg cttggccata
    13861 tttcaccgcc atatcaccaa aggtttggtc aataactaat acgatttttt ctttgataga
    13921 ggggatttca ctaaaatcta atgcgtgatt atatttagaa agctgataag ttaagatttg
    13981 agataaagct ttctctgcaa gcttaatttg ctcttgatcc tgcaaaggct gttcaagcag
    14041 gaatttttct aaacgggaag gacaagtagt atcgtaataa atcccgacat cgtcgtacac
    14101 tagagaaaaa ggcggatagc cttttacacc caagcctaat gagcgtaaaa agccgtcttc
    14161 taatgcaata taaggaagtt ggtgtttttg tgcataagct cttgctccca ccgctgaagg
    14221 acgaagcccc cagccgatga tttgatctac cttagcctta ttacctaatt tcttacgcca
    14281 agaaggtatt cgaataatac gacaatcagg tagaaaatgt tctaaattcg gaatacgaca
    14341 aattccgttc gaaaaaataa gggaaatgag catacttaat aattatcttt aaatttatta
    14401 aacgttctct gcaaattttc aaaaactaac aactatgcaa taacccaatt tctagcagac
    14461 ctaatatttt ttataaaatt attcctacgt aatttttgcc aaaaaattcg caatcttttt
    14521 gaataaaatc taattcactg cttaatttac tttaggtata tcatctatat aatgtgtttt
    14581 agtcccgaat aattatttct ggcatataaa actaactatc aaggaattta tataatcgca
    14641 tattactatt ttgatatatt tcccaaattt catctattaa atccgttatt cctcctaatg
    14701 cagctagatc aggatcaact gtaagattac ttaaccattc tttagtcttt ccttcaggaa
    14761 cagttaaagg tattactgtt gcatgcccag aataaggtaa ttccacaagt tcaaaatgtt
    14821 ttaactctct tgcttcgatt aaatccattg taaaatttct cccatagaaa acaaatactt
    14881 tattttgaga agaatttaaa ataatattct tgataggttt aacattctgt accttatgct
    14941 caattaatgt attacaatca atatattctt gtaatttcct atagctagga atatttagat
    15001 ttctattaaa cggatatata tttacttgag gagagaaagc aatacatctt atctcaacac
    15061 ttggaatatg attttgaata taatcgaaga gatacaaagc ccctgttccc cccttagata
    15121 aacctacaat acatattttt ctataattat tatcattaca taatttctct ataagcttac
    15181 caattttatt atacgatagc atatagtaag agtgagaatt atctgttaaa aacaacttat
    15241 cagaagaata ctcatacttt tgcaatgcaa aattatttgt tgcagataat gtaactaata
    15301 atgttgatga tttattattt tcatacttat aataaaaagt attatctaat tctttcaaat
    15361 tatatttttc atattcgtta tctcttttac agcactctgc cattgtcata taatcaagag
    15421 aataaataat actatctgac tcctctaatc tttttaaatt cttaaaacat tcatcgtaca
    15481 ttccataact atattgaata taagatactg aacgaagcca gttcatatta tttcttttta
    15541 ctgcaacact ctctatttca gtaatttctt ttatggttaa atttctatgt aatctatgtt
    15601 ttaatacaat acttttaaat ttagc
//
LOCUS       DANKBV010000050        15121 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.050, whole genome shotgun sequence.
ACCESSION   DANKBV010000050 DANKBV010000000
VERSION     DANKBV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15121
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.050"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(85..729)
                     /gene="adk"
                     /locus_tag="PWY43_001265"
     CDS             complement(85..729)
                     /gene="adk"
                     /locus_tag="PWY43_001265"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL2136148.1"
                     /translation="MKVILLGAPGAGKGTQAQFIVNKFGIPQISTGDMFRAAIKEGTE
                     LGKQAKALMDEGKLVPDELTVALVKDRIAQPDCANGFLLDGFPRTIPQADALKEAGVK
                     IDLVLEFDVADEVIVERMSGRRVHQPSGRTYHVVYNQPKVEGKDDVTGEDLIIRQDDK
                     PETVLERLAIYHKQTKPLITYYTAEAEAGNTRYERLDGTKPVEEVSAELAKILS"
     gene            complement(840..2972)
                     /locus_tag="PWY43_001266"
     CDS             complement(840..2972)
                     /locus_tag="PWY43_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL2136149.1"
                     /translation="MNTLSKAIKLALYGSSIGSISTAYAETVTHLNTVEVSAETPNAS
                     SEETGRYAQKKAKGSNGMALSAKDTPQSVTAVTHQQIRDQNLNTIAKALEATHGVSVS
                     LVDRGRYSFSARGFGIDKVKVDGMDLKVNNQWTTGENTGNSVLYDRVEVVRGATGLTT
                     GAGDPSASVNLVRKRANSTSRYTILEAGIGRYADFNTMIDHSQALNNSGSVRGRFIAE
                     HKNGNTFIKNEKERYTTLYGNIEADLTDTTKLGLGVSYQKEKRDAALWGGLPAFYSDG
                     GETNWSRSKNASPEWAYWDNESVNYFADITQKLGEKWELALKGNYRVGKHNSELFYFS
                     GSSLNRQDGLGWSPWPGKFKNDNTQSNVQLDLTGSFSAWGLEHDAAIGIQYNRAHRNS
                     YAADNSTFDGAFDFNNWNGIYARPNWGSMSEKYYQNLKEFGVYVSTRLRLTDRLSMLL
                     GSRISSYRTEGTFYGSTQNFHANDVGTPYAGLTFDINPQNTVYVSYTDIFKPQSQVDI
                     NNKLLSPVTGETYEAGWKGSYFNDNLQTQISVFETRQDNLAQSAGINPNTLLAYYTAS
                     KGAKVRGFEVEASGNVTEKLKLNAGYTQWKGDDASGNPINTTQPRKQFKFFATYDMNR
                     LVQGLTVGAGVNWQSRIYTKHATYGEYGQKAYTLVNLMARYQFNDNFSAQLNIDNLFD
                     KKYRNALSFSQYSYGEPFYTRLNLRYEF"
     gene            complement(3000..4964)
                     /gene="fhuB"
                     /locus_tag="PWY43_001267"
     CDS             complement(3000..4964)
                     /gene="fhuB"
                     /locus_tag="PWY43_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDL2136150.1"
                     /translation="MNKRWLNSSFLLIGCILLSLVTAVIRLNMVWEWEWQLLLINVDL
                     LPINQAAVKAALLPEMTTSFLAGGLLAIASSGLQHIVRNNLASDSTLAVSSGAQLALM
                     IAVIFFPAAELFSSFWIALLGSLAAIVLVLFIAKASKLNPLTLILGGLIVNILFGAIA
                     SLLTLFYFDFLFGVMVWGSGSLLQDGWATSIILAITVVVAFFIFVLLSRPLTILSLDD
                     EQARRLGAPVNLLRYLVIFVCAAITALVVSKIGVIGFIGFAGASLAHFAKIRHLLLRL
                     LIAFLAGGFLLFFTNNLIGIVSRIYDTLLPVGAFTATLGAPLLIFLVLRQRKAQFEAS
                     NTAYFPVKRLNSWKLPILLLCAGVILFCLLQIIAPTMNGWAISIDENLMSSYRLPRSL
                     SAIATGAMLAVSGSLLQILTRNPMASPEVLGVSSGATIAAILSFLLFPTLSSIGFLLF
                     GVLGSLAVLALIMWLSRKLQPSALMIAGVAIGALVSGLMAIIQLSGSPRLTAVISWLS
                     GSTYYANPNTVWILLSVAILLISATLLFIKPLRLISLGETVSLHLGLEVKKVQMIVLL
                     LIALMSAASTLAVGPMSFIGLMTPHLAKNLGAITPEKQIPISALLGAMLMLIADWLGR
                     YLIFPYEIGAGIIASILGGIYFLLLLKQHK"
     gene            complement(4945..5412)
                     /locus_tag="PWY43_001268"
                     /pseudo
     CDS             complement(4945..5412)
                     /locus_tag="PWY43_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
     gene            complement(5409..6197)
                     /locus_tag="PWY43_001269"
     CDS             complement(5409..6197)
                     /locus_tag="PWY43_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2136151.1"
                     /translation="MLLKHNTMIQINSISYSVEQNRKLLSNISLNLSKGRLYGVIGHN
                     GSGKSTLMRLLSGDITPTSGSILVDGLPLHQLSTKEAAKHIAYLPQRLPEAADFLVPE
                     LVALGRFPYQKWLQKPTEFDVQIVEQAMESTSVTQFAKQPVNSLSGGEKQRVWLAMCL
                     AQQTPYLLLDEPLAALDIVYQVEVMQLIRKLVNEQGLTVIIIIHDINLASQFCDEIIA
                     LKQGELCIQGSVDEIMQEQKLLEIFGLKLHLFNHPAGLHKVAVL"
     gene            complement(6429..7712)
                     /locus_tag="PWY43_001270"
     CDS             complement(6429..7712)
                     /locus_tag="PWY43_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2136152.1"
                     /translation="MTACHEPKRSIWQQIFTKNMLLCVYTGFCSGLPLFVLIQLMPAW
                     LTSANLDIKTIAAFTLTSLPYTWKFLWAALLDRYFPPFLGRRKSWIFISQIGLLVILA
                     SFGLFNPVQDISIIVTLSVLLAFLSATQDIVVDAFRREILSDNELGLGNSIHVNAYRI
                     AGLIPGGLSLFLADHYAWDTVFLITAAFLVPCLFVTLIIAAEPNHKPIDRSKPFYMAF
                     VDPFKEFFTRKGVAGAIGLILFIFLYKLGDSLATTLQTKFILDMGFTKSHIAGIVKIT
                     SLWCSIGGGIIGGVAMLKLGVNRALWLFGFVQLITILGFAYLASFEHFPTESIGAAEL
                     WKLGLVMASEYLGVGLGTAAFVAFMARETNPAYTAMQLAIFTSLAALPSKMLGAYTGN
                     LVEMMGYYQFFWLCFFIGIPGMLMLFKVAPWGQAE"
     gene            7878..11593
                     /gene="metH"
                     /locus_tag="PWY43_001271"
                     /pseudo
     CDS             7878..11593
                     /gene="metH"
                     /locus_tag="PWY43_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            11701..13704
                     /locus_tag="PWY43_001272"
                     /pseudo
     CDS             11701..13704
                     /locus_tag="PWY43_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250042.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            13803..>15121
                     /locus_tag="PWY43_001273"
     CDS             13803..>15121
                     /locus_tag="PWY43_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2136153.1"
                     /translation="MDSTFFLKKYLNHPSFNFPLSLNEVSLIKSGTTPTDRDDNLKEG
                     VVLLKTNDIRNNLLNKYSSVDYFISEDINEKMISSQLKEGDVLVNIVGATLEVVGRVA
                     YVSSTFPKANITQAMSFVRLKSKYNKELLPTYLFAFLQSSYGKIQINRNARPTGQYNL
                     NNEELGAIKVPLIDLETQKQVDKIIKQSNDFVQKSTQSYQEAETLLLENLGLRAFQAD
                     SNPVNVKSLKESFLQTGRLDAEYYQTKYEQYWNLIQSQDYVFIRDEYLHITQKPDWTK
                     PMYQYIEIGDVNIGDGSYQTNWIETQELPANAKTQAQTGDILISTVRPYRGAVTIIGE
                     NDQDLVVSGAFTVLRRKENSVFNNEVLKVLLRSELYKDWLLQFNVGTSYPVIKDNDIL
                     NLPIPKISGEIQEKIAEYIRQSNDLRQQAQNLLAQAKNNVEFEIENNS"
BASE COUNT         4735 a         3032 c         3216 g         4138 t
ORIGIN      
        1 gctcaccaaa tattgtgttt aagccaaata acaagcggta atattctcga aaaatattac
       61 cgcttgttca ttttaataat ccgtttaaga taaaattttc gctaactcag cgcttacttc
      121 ttctaccggt tttgtaccgt ctaaacgctc ataacgagta ttgccggctt ctgcttctgc
      181 ggtgtagtag gtgattaacg gcttggtttg tttgtggtaa atcgctaaac gctctaacac
      241 ggtttcaggt ttgtcgtctt ggcggataat taaatcctca ccagtcacat catctttacc
      301 ttccactttc ggttgattgt acaccacgtg gtaagtacgc cctgatggtt ggtgaacacg
      361 acgaccgctc atacgctcaa caatcacttc atcggctaca tcaaactcaa gtactaaatc
      421 aatttttacg ccagcctctt ttaacgcatc agcttgcgga atggtgcgtg ggaaaccgtc
      481 taataagaaa ccgttcgcac aatcaggttg agcgatacgg tcttttacta aggcaaccgt
      541 taattcatcc ggcactaatt taccttcgtc catcaatgct tttgcttgtt taccaagctc
      601 agtgccttct ttaatcgccg cacggaacat atcgccggtt gaaatttgcg gaataccgaa
      661 tttgttcaca ataaactgag cttgcgtacc tttgcccgca cccggtgcac ctaataaaat
      721 aactttcatt gtaaacctca ataactagaa aaatgaataa ataacttagg cagtaacctt
      781 accgattact gcctaaaatt gcaaattttt ttaatttttt gaccgcttgt tatacggaat
      841 tagaattcat agcgtaaatt taagcgtgta tagaatggct caccgtagct atattggcta
      901 aagctgaggg cgttacgata ttttttatcg aataagttat caatgttaag ctgtgctgag
      961 aagttgtcat taaattgata acgagccatc aaattcacta aagtgtaagc tttttgacca
     1021 tattcgccat aagtagcgtg tttggtataa attctgcttt gccagtttac acctgctcct
     1081 accgttaaac cttgcactaa acggttcata tcgtaagttg caaagaattt aaattgtttg
     1141 cgtggttgag ttgtgttaat tgggttgcct gaagcatcat cgcctttcca ctgagtatat
     1201 cctgcattga gttttaattt ttcagttaca tttccagatg cttctacttc aaagcctcga
     1261 actttcgcac ctttagaggc tgtgtagtag gctaataatg tattagggtt aataccggca
     1321 gattgagcta gattatcttg tcttgtttca aatacgctaa tttgagtttg caagttgtca
     1381 ttgaaataac tccctttcca acctgcttcg taggtttcgc ccgtaacagg agagagtagt
     1441 ttgttattga tgtccacttg actttgtggt ttgaaaatat cggtataact tacataaacg
     1501 gtattttgtg gattaatatc aaaggttaat cctgcataag gtgtcccaac atcgttagcg
     1561 tggaagtttt gtgttgaacc atagaaagtg ccttctgtgc ggtacgatga aatacgagaa
     1621 cctagaagca tagaaagtct atctgtaaga cgtaaacggg tagaaacata caccccgaac
     1681 tcttttaagt tttggtagta tttttcagac atacttcccc agtttggtct agcataaatg
     1741 ccattccaat tattaaagtc gaaagctccg tcaaaggtgc tgttatcggc agcgtaggaa
     1801 ttacgatgtg ctcgattgta ttgaataccg attgccgcat cgtgttctaa tccccaagca
     1861 gagaatgaac cggtaagatc aagctgcaca ttagattgtg tattatcatt tttaaattta
     1921 cccggccaag gcgaccaacc taatccatct tgtcgattaa gtgatgagcc gctgaaatag
     1981 aaaagttctg aattatgttt gcctacacgg taattgcctt ttaatgcaag ttcccatttt
     2041 tcgcctagtt tttgtgtaat atcggcaaaa tagtttacgg attcattatc ccagtaagcc
     2101 cattctgggc tggcattttt gcttctgctc cagttagttt caccaccatc gctatagaat
     2161 gcgggtaaac caccccaaag tgctgcatca cgtttttctt tttgataact tacacctaac
     2221 ccaagtttgg tagtgtcagt taagtctgct tcaatattgc cataaagcgt ggtgtaacgt
     2281 tctttttcat ttttaatgaa cgtattaccg tttttatgct ctgcaataaa gcggcctctt
     2341 actgatcctg aattattcaa tgcttggcta tggtcaatca ttgtattgaa atcagcataa
     2401 cgcccgattc cagcttctaa aatagtataa cgggaagtgc tattggctcg tttacgaaca
     2461 agattaacac ttgcagaggg atcgcccgcc ccagtagtta agcctgtagc accacgcaca
     2521 acttctactc ggtcgtataa cacgctgttg cctgtatttt cgcccgttgt ccattggtta
     2581 tttactttga gatccatacc atctaccttg actttatcaa tcccaaaacc acgagcactg
     2641 aaagaatagc gtcctctgtc aactaaagaa acagaaacac cgtgagttgc ttcaagtgct
     2701 ttggcaatcg tattaagatt ttgatctctg atttgttgat gcgttacagc cgttacgctt
     2761 tgtggcgtgt ctttcgcact caatgccatt ccattagagc ctttggcttt tttctgtgca
     2821 taacgtccag tttcttccga tgaggcattt ggcgtttcgg cacttacttc aacggtatta
     2881 agatgagtaa ctgtttcagc ataagctgtt gagatagacc ctatactaga accgtataag
     2941 gcaagtttta tcgctttaga tagcgtgttc ataatttctc ctaagtaatg aataaaaaat
     3001 tatttatgtt gtttgagtaa cagtaagaaa tagatacccc cgagaataga ggcaataatg
     3061 cctgccccaa tctcataagg gaaaattaga taacgcccga gccaatcggc aatgagcatt
     3121 agcattgccc ctaataatgc cgatataggg atctgtttct ccggtgtaat tgcacctaaa
     3181 tttttagcta aatgaggggt cattagccca atgaagctca ttggccctac agcaagcgta
     3241 gaggcggcac tcattagggc gattaaaagt aatacaatca tctgcacctt tttaacctct
     3301 aaccctaagt ggagagaaac ggtttcgcca agtgagatca agcgtaaagg tttgataaac
     3361 agtaaagtgg cactgataag taggattgca acagataata aaatccaaac tgtattcgga
     3421 ttagcgtaat aggttgagcc agacagccaa ctaataactg ccgttaaacg agggctgccg
     3481 cttagttgaa taatagccat taagccactg actaaagccc caatagcaac acctgcaatc
     3541 attaacgctg atggttgtaa tttacgggaa agccacataa taagagcgag aacggccaaa
     3601 ctccctaata caccaaataa caagaagcca atgctagata atgttgggaa aagtaagaat
     3661 gacagtattg cagcaatagt tgcccctgaa ctcacaccta atacttcagg gcttgccatt
     3721 gggtttctcg ttaagatttg taataggcta ccacttaccg caagcattgc ccctgttgct
     3781 atcgcactta gactacgagg caagcggtag gaactcatca ggttttcatc aatgctaata
     3841 gcccagccat tcattgttgg agcaatgatt tgtaatagac aaaataggat aactccagcg
     3901 cataacaaca atattggcag tttccacgaa ttgaggcgtt taacggggaa gtatgctgta
     3961 ttgcttgctt caaattgtgc tttacgttgg cgtaatacca agaaaattaa taatggtgcg
     4021 cctagtgttg cggtaaatgc accaacaggt agtagagtgt cgtaaattct gctaacaatg
     4081 ccgatgagat tattggtaaa aaagagtaaa aatccgcccg ctagaaaagc aatcaagagg
     4141 cgaagtaata aatgtcgtat tttagcaaaa tgagccaaac ttgcacctgc aaatccaata
     4201 aagccaatca cacctatttt gcttacaacc aatgctgtaa tggcggcaca aacaaaaata
     4261 actaagtagc gtagtaaatt aacaggcgcg cctaagcggc gagcttgttc atcatctaaa
     4321 cttaaaattg tgagtggacg agagagtaag acaaagataa aaaatgcaac tactaccgtt
     4381 atcgctaaga taatagaagt tgcccaacca tcttgtaaaa gtgagccact tccccaaacc
     4441 atcacgccaa ataaaaaatc aaagtagaaa agggtcagta aagaggcaat agctccaaac
     4501 aaaatattaa caatcaatcc ccctaaaata agggtaaggg gattgagctt agaggcttta
     4561 gcaataaata aaaccagtac gatagcagct aaagagccaa gtaacgcaat ccagaaacta
     4621 gaaaaaagct cggcggctgg gaaaaagata accgcaatca tcaatgcaag ttgagccccg
     4681 ctactgacgg caagtgtgct atctgacgct aaattatttc tgacaatatg ttgtaagcca
     4741 ctgctggcta tagccaataa accgcctgct aaaaatgacg ttgtcatttc aggtaaaaga
     4801 gctgctttta ccgcagcttg attaattggc aataaatcaa catttatgag tagtaactgc
     4861 cattcccact cccagaccat atttaaacgg ataacagctg ttactaagga taaaagaata
     4921 cagccaatca ataagaatga tgaattgagc caacgtttgt tcagttttcg ttaaatatgc
     4981 ttctgccgca ttagggtttt taatctctgt tccaatcata cgagtggctt tttggtaagt
     5041 atgccaatgt tgctgaggtt cagtattgcc accatcaaat agcacgtcta tttgtggaat
     5101 ttctttcggg taaacagaag ccaagtgagc gtagaaaaag tttgatagca ccaattcaac
     5161 aggaagctgt gctaataatt cgcgattagg ctgataacgc gctccaatat ctaacacgct
     5221 attaggctga atagggcggt gcgaccatag cggataaatt cgcttatccc ccatcgcaat
     5281 gggcggattt cccatttcag taagggcaga ggcaatagac caatctggtg taaaaatatt
     5341 aggttgagca aatgcggtag aaatttgcaa aataaagaga aaactgaacc gcttgtattg
     5401 cctaaacatc ataataccgc cactttatgt aagcctgcag ggtggttaaa caagtgcagt
     5461 tttagcccga agatctcaag taatttctgt tcttgcataa tttcatcaac agaaccttga
     5521 atacagagtt cgccttgctt taacgcaata atttcatcac aaaattggga ggcaagatta
     5581 atatcgtgaa taatgataat gacagttaga ccttgttcat tcactagctt gcgaataagc
     5641 tgcattactt caacttgata aacaatatca agggcagcca atggctcatc tagcagtaaa
     5701 tagggtgttt gttgggctaa gcacatagca agccaaactc gttgtttttc tccgcctgat
     5761 agactattta caggctgttt agcaaactga gtaacggagg tgctttccat tgcttgctca
     5821 acaatttgaa catcaaattc tgttggtttt tgtagccatt tttgatatgg aaatcgccct
     5881 aaagcaacca gttccggcac taagaaatcc gccgcttcag gtaaacgttg tggtaaatag
     5941 gcaatatgtt ttgccgcttc tttggtagaa agctgatgaa gtggtaatcc atcgactaaa
     6001 attgaaccgc tggtaggcgt tatatctccg cttagtaaac gcattaacgt tgatttaccc
     6061 gagccgttat ggcctattac gccatataaa cgccccttgc ttaaattgag ggaaatattg
     6121 cttagcagtt tacggttttg ttcaacagag tagctaatgg aatttatttg aatcatagtg
     6181 ttgtgtttta agagcataaa aaaggtggga ttaccccacc aaatcaatca aaatgaaaat
     6241 gagtagatga aaatctatta tgaaaacggt gctaaatata tttttattat aaatgaaagt
     6301 caatatcatt tagttttagg tgagggtaaa ttttaggata aataaaaaat aaccgactaa
     6361 attactaggt aattcaatcg gttataagtg gtcggatatt taataaaaaa tcttattttt
     6421 atgaatagct attcagcttg tccccaagga gcaaccttaa acaacattag cattcccgga
     6481 ataccaataa agaaacagag ccagaagaat tggtaatagc ccatcatttc cactaaattc
     6541 cctgtgtatg cccctaacat tttgcttggt agggcagcaa ggctggtgaa aatggcgagc
     6601 tgcattgcag tgtaagcggg attagtttca cgagccataa aggcaacgaa agccgctgtg
     6661 cctaaaccca cgcccagata ttcacttgcc atcactaagc ctaatttcca aagctcggca
     6721 gctccaatgc tttcggtagg gaaatgctca aaactggcaa ggtaagcgaa gcctaaaata
     6781 gtaattaatt gtacaaatcc gaacagccat aaggctcggt taacgcccaa tttcaacatt
     6841 gccacgccac caataatacc gccgccaatg ctgcaccaaa gtgaagttat tttcacaatg
     6901 ccggcaatgt gcgatttagt aaaacccata tccaaaataa atttggtttg tagcgtagtt
     6961 gcaagggaat cgcctaattt atataggaaa ataaatagaa tcagcccaat cgctcctgcc
     7021 acgcctttac gggtgaaaaa ctctttgaac ggatcgacaa acgccatata aaacggctta
     7081 gaacggtcga ttggcttatg attcggctcg gcagcgatta ttagcgtaac gaataagcaa
     7141 ggcactaaaa aagcagcggt aatcagaaat acggtatccc acgcatagtg gtcggctaaa
     7201 aataatgaaa gcccacccgg aatcagccct gcaatacgat aagcattaac gtggatagag
     7261 ttacctaacc cgagttcgtt atccgataaa atttcacgcc gaaaggcatc caccacaatg
     7321 tcttgcgtgg cggaaagaaa agctaatagc acagaaagag taacaataat tgaaatatct
     7381 tgcactgggt tgaacaagcc aaagctggct aaaatcacca atagcccgat ttgtgaaatg
     7441 aaaatccagc ttttgcgtct gcctaaaaaa ggtggaaagt agcgatctaa caatgcagcc
     7501 cacaggaatt tccacgtgta gggcaaagag gtcagggtga aggcggcaat agttttaata
     7561 tctaaattgg cactggttag ccaagccggc attagctgaa ttaacacaaa caacggtaaa
     7621 ccggaacaaa agccggtgta aacacaaagc aacatatttt tagtaaaaat ctgttgccag
     7681 atagagcgtt tgggttcgtg acaagcggtc atatttctcc tacattttgc aaaataaaaa
     7741 ggggaagaac tgagccaata tagcataatt ttaaaggcga ctctaaccct ttccatttcg
     7801 tcacagataa ggtttgacag ccttgcgaca tatttctatt attcctttta atcaaactaa
     7861 aaaactggaa gcagagtatg aacacgcagc atagaaccga attacttcaa aatgccctta
     7921 ccgaacgcat tcttatctta gacggtgcga tgggaacgat gatccaaaaa tataaactaa
     7981 ccgaatctga tttccgaggt gaacgtttta aaaacagtac catcgatctt aaaggcaata
     8041 acgacctact cacactcacc cagccactcc tgatttctgc tattcacgag aaatatttag
     8101 ccgccggtgc ggatattatc gaaactaata cctttagtgc gacgaccatt gctcaagcag
     8161 attatgaact tgaaagtatt gcttatgggc tgaatcttgt gggggcaaaa cttgcccgtc
     8221 ttgccgccga taaattctcc acccctgaaa aacctcgttt tgttgccggt attcttgggc
     8281 cgactaaccg tacttgttcg atttcccctg atgttaatga ccccggtttc cgtaacatta
     8341 cctttatgca actggtggaa gcctatgccg aggcaacccg tggtttgatt gaaggtgggg
     8401 cggatctgat tatgatcgaa accattttcg ataccctgaa tgccaaagcg gcggtgtttg
     8461 caattgaaac agtgtttgat gaactgggcg tgaaattgcc gataatgatc tccggcacca
     8521 ttaccgatgc ctcagggcga acgttgtcag ggcagaccac ggaggcgttt tataactcgc
     8581 tccgccacgc caaaccgctg acgttcggct tgaactgtgc gctcgggccg aaggaactcc
     8641 gcccttatgt agagatgatg tcgaaaatca gtgagacttt tgtttcggtt cacccgaatg
     8701 ccggcttgcc aaacgctttt ggcgggtacg atttaggggc ggaagagatg gcgacttaca
     8761 tcaaagagtg ggcagaactc ggctggctga atattgtcgg cggctgttgt ggcaccacgc
     8821 cggagcatat ccaagccttt gccgaagcaa ccaaaggcgt tgcgcctcgt aaattacctg
     8881 aaattccaac cgctatgcgt ttgtcgggct tagaaccgct gacgattgac gataattcgc
     8941 tgtttgtgaa tgtgggcgaa cgcaataacg tcaccggttc ggcaaaattt aagaagctga
     9001 ttaaagaaga aaaatttgcg gaagcgattg aaattgccat cagccaagtg gaaaacgggg
     9061 cgcaggtgat tgacgtgaat atggacgagg cgttgctcga ttccaaaaaa tgtatgaccc
     9121 gtttcctcaa cattcttgct accgaacctg aggcggcaaa agtgccgatt atgattgact
     9181 cctcgaaatg ggaggtgatc gaggcgggct tgcaaacggt gcagggcaag ccgatagtga
     9241 actccatttc actcaaagag ggcgaagaga aatttattca ccaagccaaa ctctgccgcc
     9301 gctatggggc tgcggtggtg gtgatggcat ttgacgaagt cgggcaagcg gataccgaag
     9361 aacgcaaggt agaaatttgt acccgagctt atcggatttt ggttgatcag ctcggcttcc
     9421 caccggaaga cattattttc gacccgaaca tttttgcgat tgccaccggt attgaagaac
     9481 acaataacta cggcgtggat tttatccaag cctgtgagcg gattaaacga gacttgccac
     9541 acgccaaaat ttccggcggg gtgtcgatgt ctctttctct ttccgaggca acaatgtggt
     9601 gcgtgaggct attcacgccg tgttcctcta ctatgcgatc aaagccggaa tggatatggg
     9661 gatcgtgaac gcagggcagt tggcgattta tgacgacctc gaccctgaac tgcgagaggc
     9721 gattgaagat gcggtgctga accaccgtca tgacgctacc gaccgcctgt tggaaatttc
     9781 cgaaaaatat cggggcgtaa aagtagaaag tgccgatgaa agtgccgccg agtggcgaaa
     9841 tttacctgtg gcagagcgtt taaaacacgc cctcgtaaaa ggcataacga cctacattat
     9901 tgaagatacc gaagaggctc gccagcaatt tgcctctccg ctggaagtga ttgaagggcc
     9961 gctcatggcg ggtatggacg tggtgggcga cctgttcgga gacggcaaaa tgttcctgcc
    10021 gcaagtggtg aaatcggctc gggtaatgaa acagtccgtt gcctatttag agccgtacat
    10081 caacgccacc aagcagaaag gatcgagcaa cggcaaaatg gtgatcgcca ccgtgaaagg
    10141 tgatgtacac gacatcggca aaaacatcgt tagcgtggtg ttgcagtgca ataactttga
    10201 agtgatagac ttaggtgtga tggtgcctgc cgatgtaatt atcgaaactg ctattcggga
    10261 gaaagccgat ctcattgggt taagcggact aattacccca tcgctggacg aaatggagta
    10321 ttttttaggt gaaatgaacc gcttgaagct cgatattccg gtgctgattg gcggcgcaac
    10381 gacctcaaaa gagcatacgg cgatcaaact ttatccgaaa tataactacc ccgtagtcta
    10441 taccgccaac gcttcacgtt gcgtaaccgt ctgtgcgacc ttgatgaacc ctgaggcaaa
    10501 agccgaattt tgggaaaaaa ccaaagccga atacgaagaa attcagcgta aatttgccgt
    10561 tcgcaaacca cttcgcaacg gtttatctat tgaggaggct cgagccaatc gttttgacgg
    10621 atttagtggc gaatgggcag actatgtgcc gccaacgcca aaacagaccg gcattatcga
    10681 gtacaaaaat gtaccgattg ccaccctacg caaatttatc gactggtcgc cgttctttat
    10741 gctctggggc ttaatgggcg gttatcctga tgcctttgat tatccggaag gcggcgaaga
    10801 ggcacgccgt gtctataacg atgcccaacg ggtgctggac gaatttgagc aaaacggcaa
    10861 gctcaaccca agtgggatta tgggcatttt ccctgccgtg cgtgagggcg atgatattgc
    10921 aattttaaac gaagatcgca ccgcttgtat cggcaaatcc taccacctcc gccaacaatc
    10981 cgaacgaggc aaaaacagca aaagcccgta caacctctgt ttaagcgatt ttatcgccga
    11041 ccgagaaagc ggacaacaag actggttcgg aatgttcgcc gtttgtgccg gcattgaaga
    11101 acactattta gttgaaagct acaaagcggc aggcgatgac tacaacgcca ttttgctcca
    11161 agccgttggc gaccgcttgg cagaagcaat ggcggaatac ctacactttg agctacgcac
    11221 caaagtctgg ggctacaccg tggaagacga agacaacgga cgcttaatcc gtgaagaata
    11281 catcggcatc cgccctgcgc ccggctaccc aagctgcccg gaacacaccg agaaacagat
    11341 aatttgggac ttgttagagg tggaacaacg catcggcatg aaactcaccg aaagctatgc
    11401 catgtggccg gcagcaagtg tctgcggctg gtacttcacc caccctgcca gcaactactt
    11461 cactctcggc aagattgatg aagatcaggt gttggattac gccaaacgca aaggctggga
    11521 cgaactcacg gcgaagaaat ggttaagtgt ggcgatgaaa caagatgtca agtcgcaggc
    11581 tgaaaacatt taatgtagtg gcgggtttta acccgcctgt tacaagcggt tggttttgag
    11641 ataaaatatg caaaatttat ctctctttta tgcaaagaag ttatcatcga gaacaaaaca
    11701 atgattgaaa aactaaatta tcacttagta caacttttaa aagacagtaa ttatcaatta
    11761 actcaatttt ctcctgcgta tataaaaaaa ttagaggaat ctattttcct caaatcaggg
    11821 aaaaatggag atattcctca tattcaatgt cttgttcgta aaaaggatat taagctcaca
    11881 cctgaagaag cagtacgcca actctattta atgaatttgc attatgaata tgattatcca
    11941 tttgaacgaa tggaattaga atattctgtt gtattcgggc gtgagaagaa acgagcggat
    12001 attgtcatct ttgatagaga taatcctaac gctgtctata ttatggtgga gctgaaaaag
    12061 ccgaaattaa aagatggtaa agaacaatta aaatcttatt gtaatgcgac tggagcacct
    12121 attggtgtat ggacaaatgg cgaaagtatt tcttactata atcgccgtga tcctaattat
    12181 tttaaagata ttcccgatat tccgaatgct caacaaaaac tttctgattt attacaagaa
    12241 cgttggacta ttcaagatct gatagataat gacaaaattg ccaaacaacg taaatcattg
    12301 aaagatcttg ttattgaaat ggaagatgag gtactggcga atgctggagt tgatgttttt
    12361 gaagagttat ttcaacttat ttttactaaa ctctatgatg aaatggaaag tacacgcaat
    12421 aaaagtcgtt atttagagtt tcgtaactat ggtgataccg aaagtgaatt aaaggcaaaa
    12481 attcaatctc tatttgatcg tgcaaaaaat aaatgggaag gcgtatttgc tgaaagtgcc
    12541 aaaatcaacc tttccccttc gcatttggcg atttgtgtat cttcattgga agaagtaaag
    12601 ctgtttaatt ctaaccttga tgtagtggat gaagcatttg agtacttaat taataaatcc
    12661 agtaaaggag agaaaggaca atactttacg cctcgttacg tcattgatat gtgcgtaaaa
    12721 atgctcgatc caaaacctga agaaacagtc attgataccg cagcaggaag ctgtggtttt
    12781 ccagttcatt ctatttttca cgtttgggaa aaacaactta aagcgagagg attagaacgc
    12841 tcacacttat ttactgctga agaaaaattg cctgaacaaa cagattatgt gaaagaaaaa
    12901 gtctttgcga ttgatttcga tgaaaaggca gtacgtgttg ctcgtaccct aaacttaatc
    12961 gcaggagatg ggcaaaccaa cgtactacat ttaaatacct tagattatga acgttgggaa
    13021 gattttacta aggaagaaga atggaatgat gtatatggag aaggctggaa aaaattaaga
    13081 aaattacgta aaactaaaaa tgaaaatcga gatttccaat ttgatgtgct aatggcaaat
    13141 ccgccttttg ccggtgatat taaagaaact cgtatatgag ctcgctatga attaggcaaa
    13201 aatagcaaag gaaaacagca atccaaagtc ggacgagata ttttatttat tgaacgcaat
    13261 ttagatttct taaaagacgg tggacgaatg gcgattgtgc taccacaagg gcgttttaac
    13321 aattccagcg ataaatatat tcgtgatttt attgcagagc gttgccgcat tttagctgtt
    13381 gtgggcttac acggaaatgt gtttaaacca cataccggca caaaaacttc cgtactattt
    13441 gtgcaaaaat ggcacgatga actttgcccg aaagtagagg attacccgat tttctttgcc
    13501 acaatgcaga aatccagcaa agataattca ggcgataaaa tctatgttaa aaacgctgat
    13561 ggcacactca aattagacaa ccatcaacac ctaattgtgg agcacgatct atttaatcac
    13621 gataatagta cccaagacgg cattgcagaa gcctttattg aatttgctaa aaaagaaaag
    13681 ctctcttttg tgggaaagga ttaagcccat ttgaccctgc taaatatcag gcattgttag
    13741 atgggctaga ggtgagcgag gtttatttga gtaaggttcg ttctgcaagt tattcggaac
    13801 gaatggatag tactttcttt ttgaaaaaat acttaaacca tccgagtttt aattttccat
    13861 tatcgctgaa tgaggtttct ctgattaaaa gtggtacaac accaacagat cgagatgata
    13921 acttaaaaga gggggttgtc ttattaaaaa ccaatgatat taggaataat ctattaaata
    13981 agtactcttc ggtagattat tttattagcg aagatatcaa tgagaaaatg atttcatcac
    14041 aactgaaaga aggggatgtt ttagtaaata tcgttggtgc aactttggag gttgttggtc
    14101 gggtagctta tgttagtagc acttttccaa aagcaaatat tacacaagca atgtcttttg
    14161 taagattaaa atcaaaatat aataaagaat tattaccaac gtatttattt gcttttttac
    14221 aaagttctta tggaaaaatc caaattaacc ggaatgcacg tccaacagga caatacaatc
    14281 tgaataatga ggagcttggt gcgattaaag tccccttaat tgatctagaa acacaaaagc
    14341 aggtagataa aatcattaag caaagtaatg attttgtaca aaaatcaaca caaagttacc
    14401 aagaagctga aactttatta ttagaaaatc tcggtttgag agcttttcaa gctgatagca
    14461 atcctgtaaa tgttaaatcc ttaaaagaga gtttcttaca aacaggacga ttagatgcag
    14521 agtattatca aactaaatat gagcaatact ggaatttaat tcaatcgcaa gattatgttt
    14581 ttattcgtga tgaatattta catatcaccc aaaaacctga ttggacaaaa ccgatgtatc
    14641 aatatattga aattggtgat gttaatattg gtgatggttc atatcaaacg aattggatag
    14701 agacacaaga attacctgct aatgctaaaa ctcaagccca aacaggtgat attttaattt
    14761 caaccgttcg cccttatcgt ggtgcagtaa caattatcgg tgaaaatgat caagatttag
    14821 ttgttagtgg tgcgtttaca gtattacgca gaaaggaaaa ctctgttttt aataatgaag
    14881 ttttaaaggt gctattacgg tcagaactat ataaagattg gttactacaa tttaatgtag
    14941 gtacgagtta tcctgtaatt aaagataacg atattttgaa tttaccaatt cctaaaataa
    15001 gtggagaaat ccaagaaaaa attgctgaat atatccgcca aagcaacgat ttaaggcagc
    15061 aagcacaaaa tttactcgct caagctaaaa ataatgtaga gtttgaaatt gaaaataatt
    15121 c
//
LOCUS       DANKBV010000051        15116 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.051, whole genome shotgun sequence.
ACCESSION   DANKBV010000051 DANKBV010000000
VERSION     DANKBV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15116
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.051"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..462
                     /gene="trmL"
                     /locus_tag="PWY43_001274"
     CDS             <1..462
                     /gene="trmL"
                     /locus_tag="PWY43_001274"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL2136154.1"
                     /translation="YEPEIPQNSGNIIRLCANCGFRLHMIEPFGFGWDDKKLRRSGLD
                     YHEFVDIQKYKNFEDFLERAKPKRLFALTTRGEQNHSDVKYELGDFLMFGPESRGIPK
                     PLLAEMPMSQKIRIPMCKDSRSMNLSNSVAVVVYEAWRQFGYQNAVAAQSI"
     gene            complement(556..1374)
                     /locus_tag="PWY43_001275"
     CDS             complement(556..1374)
                     /locus_tag="PWY43_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL2136155.1"
                     /translation="MQEIKLFEQKSVRSHYDEEKDKWYFSIIDVIAILTESLDPSAYW
                     RKLKQRLKAEGNETVTNCHGLKMPSADGKMRLTDVADTEQLFRLIQSIPSPKAEPFKQ
                     WLAKVGYERLQEINDPSQSLDRARENWQKIGRSEKWIQQRMMGQETRNKLTDYWKSSG
                     VEQPQDFAFLTNVIHQEWSGLSVQKHKSLKGLKSQNLRDHMSEAELLFTALAELSSRQ
                     IAESMQAVGVDENAQAGKQGGNIAKNARQELENKTGKSVVTGENFLPPAKNEEE"
     gene            1617..2411
                     /locus_tag="PWY43_001276"
     CDS             1617..2411
                     /locus_tag="PWY43_001276"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL2136156.1"
                     /translation="MRLIPLETDHDVSVWAARYIVDRINAFQPTKEKPFVLGLPTGGT
                     PEKTYKELIKLYQAGEVSFKNVVTFNMDEYVGLPKEHPESYHSFMFKHFFDHVDIPLE
                     NINILNGMAEDVNAECERYEEKIRFYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIK
                     TLTEDTRIANSRFFNNDVSQVPKFALTVGVGTLLDAEEVMILVTGHNKALALQACVEG
                     AVNHLWTISCLQLHRRGIVVCDEPATQELKVKTVKYFKELEKNVAR"
     gene            2492..3634
                     /gene="nagA"
                     /locus_tag="PWY43_001277"
     CDS             2492..3634
                     /gene="nagA"
                     /locus_tag="PWY43_001277"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL2136157.1"
                     /translation="MNHYALTNCVIYTGEQVLYQHALVVKEDKIDSIVLEENLPLDLQ
                     KIDLKGANLSAGFIDLQLNGCGGVMFNEEITTRTLEIMQETNLRSGTTSYLPTFITSP
                     DEGVKQAVAVMREYLSKHKNQALGLHLEGPYLSVAKKGVHRPEFIREISPEMLDFLCD
                     NGDVITKITIAAENPTADHIKRFNDAGIIVSIGHSNATFAQTKQRIAEGAAFATHLHN
                     AMSPVSNGREGGVVGAVLDSDIYTGIICDGLHVEWGNIRIAKKAKGDKLVIVSDAVAA
                     AGSDIESFIFVGKKVTVKDGKCYDEFGALGGAAITMIESIRNVVQHCDISLNEAIRMS
                     TLYPAKAIGVEETLGSLEAGKIANLAIFDNQFEVKATAVNGQLQWH"
     gene            3716..4030
                     /locus_tag="PWY43_001278"
     CDS             3716..4030
                     /locus_tag="PWY43_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HDL2136158.1"
                     /translation="MQFKDYLATFPTIDHLAGLNVINSGEVIQHIPAIEGKLGSLKLY
                     NALFAKFDGKLTASAAKQGLEWFAEHVEDAKNNVGKHPNIDLLFRVIEQDLSLSLEPI
                     VK"
     gene            complement(4109..5299)
                     /locus_tag="PWY43_001279"
     CDS             complement(4109..5299)
                     /locus_tag="PWY43_001279"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL2136159.1"
                     /translation="MTQAIESVLAELKRGVENIYSEEDLITKLKENRPLRIKLGADPT
                     APDIHLGHTVVLNKLRQFQQFGHEVYFLIGDFTGMVGDPSGKNSTRPPLSREDVLRNA
                     ETYKEQIFKILDPQKTKIVFNSEWLGKLGTEGMIRLASNYTVARMLEREDFKNRFTNQ
                     QPIAIHEFIYPLLQGHDSVELKADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPL
                     LVGLDGEKKMSKSLGNYIGVTEAPSEMFGKIMSISDDLMWDWYNLLSFRPLDEIAQLK
                     ADVENGKNPRDVKILLAKEIIARFHDEAAADAAEQEFVNRFQKGAIPDEVPEFTFEGE
                     IGLAALLKEAGLVPSTSEAIRSAQQGGVKIDGEKVEDVKQTAQKGTFVYQVGKRKFAR
                     ITVK"
     gene            complement(5662..6966)
                     /locus_tag="PWY43_001280"
     CDS             complement(5662..6966)
                     /locus_tag="PWY43_001280"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HDL2136160.1"
                     /translation="MESIIALFKGMGLMHLELGQAIMIVISLVLLWLAIARKFEPLLL
                     LPIGFGGLMSNIPEAGMAMTALDNLLHSGSAEQLAIIAAKVNSTSLDLHSIKDAIALA
                     APSVQNELEVMAGDMGYTAGVLALFYKVAIGYGVAPLIIFMGVGAMTDFGPLLANPRM
                     LMLGAAAQFGIFATVLGALGLNWLGIIDFTLPQAAAIGIIGGADGPTAIYLASKLAPE
                     LLGAIAVAAYSYMALVPLIQPPIMKALTTEQERKIRMVQLRNVSNREKIIFPIVLLLL
                     VALLLPDAAPLLGMFCFGNLLRTSGVVERLNDTAQNALINIVTIFLGLSVGAKLVADK
                     FLQPQTLGILVLGVIAFGIGTASGVIMAKIMNRFSQHKINPLIGSAGVSAVPMAARVS
                     NKVGQEYDKQNFLLMHAMGPNVAGVIGSAIAAGVMLKYISTM"
     gene            complement(6976..8802)
                     /gene="oadA"
                     /locus_tag="PWY43_001281"
     CDS             complement(6976..8802)
                     /gene="oadA"
                     /locus_tag="PWY43_001281"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HDL2136161.1"
                     /translation="MTTQPKKIAITDVVLRDAHQSLFATRLRLDDMLPIASELDNIGY
                     WSLETWGGATFDSCIRFLGEDPWVRLRELKKAMPKTPMQMLLRGQNLLGYRHYADDVV
                     DKFVERSVANGMSVFRVFDALNDPRNMQAALQAVKKHGGHAQGTLSYTTSPVHTLQTW
                     LDVTEQLLEIGVDSLVIKDMSGILTPMAAYELVSEIKKRYDVELHLHCHSTTGMAEMA
                     LLKAIEAGVDGVDTSISSMSGTYGHPATESIVATLQGTPYETGLSIPALEKISAYFRN
                     VRKKYAKFEGQLRGIDSRILVAQVPGGMLTNLESQLKQQNASDKLDLVLQEIPRVRED
                     LGNIPLVTPTSQIVGTQAVMNVLMGERYKTIAKETAGILKGEYGRTPAPVNKALQDRV
                     LEGAEPITDRPADHLPPELEKLVAEVTASAKEKGITLSENMIDDVLIVALFQQVGWKY
                     LENRNNPSAFEPVPSADEAKPAQASAASAPKAQTQSGSAIYTVELEGKAFVVKVSEGG
                     DITNIAPTPATPAPQAVDSAPKSANTLSANAEPVNAPMAGNILKVEVKEGQQVAEGDV
                     LLILEAMKMETQICAAKSGTIQGINVKQGDVVAVNDTLMSIA"
     gene            complement(8842..9096)
                     /locus_tag="PWY43_001282"
     CDS             complement(8842..9096)
                     /locus_tag="PWY43_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit gamma"
                     /protein_id="HDL2136162.1"
                     /translation="MAPAELFAEGINLMVAGMGFVMLFLLVLIYAISFISKIINQYFP
                     EPVSIPASKTVPQSPPDDLERLRPVIAAAIAHHRRINGQH"
     gene            complement(9286..10473)
                     /locus_tag="PWY43_001283"
     CDS             complement(9286..10473)
                     /locus_tag="PWY43_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL2136163.1"
                     /translation="MSNNNRSALLGAAFLMATSAIGPGFLTQTATFTQTLLASFGFVI
                     LLSILLDIGAQLNIWRIVAVSEKKAQDIANAVLPGAGYFLALLIVMGGLAFNIGNVGG
                     AGLGLNILTGLSPEMGAVVSGAIAVGVFLFREAGKVMDRFAQIMGFVMIALTIFVAVK
                     ANPPIDDAVVHTFVPEKLDVIAIVTLVGGTVGGYITFAGAHRLLDAGIKGKENLKEVS
                     KSSVSAILIASIMRVVLFLAVLGVVSMGVQLDPKNPAATPFAHVAGDVGLMIFGVVIW
                     AASITSVIGAAYTSVSFITSFSPFIEKHKNCFIVAFIVVSTAVLATIGRPAQVLVFVG
                     TLNGLILPISLGLILLAAHKTKIVGDYKHPVWMTVSGAIVVILMAVLSAMTLSKYISG
                     LMA"
     gene            complement(10748..11485)
                     /gene="pxpA"
                     /locus_tag="PWY43_001284"
     CDS             complement(10748..11485)
                     /gene="pxpA"
                     /locus_tag="PWY43_001284"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDL2136164.1"
                     /translation="MNHVDLNVDLAEGCGNDHSLLQLVTSANVCCGLHAGDYNEMRKA
                     INWAKENSVRVGAHPSFPDRENFGRTNMQLPDDELKACLLYQLGAAKALCDAENMPMS
                     YVKPHGALYNMAAKDPHLATLIANTVKQFDPNLKLMGLSGSLMLSIAEQHGLETISEV
                     FADRHYLADGSLVPRTRDDAMVESDDEAISQVLQMVLDGTVPTVDGGNVAIKADSICL
                     HGDSEHAVVFAERIKKELQAKGILVKA"
     gene            complement(11472..12401)
                     /locus_tag="PWY43_001285"
     CDS             complement(11472..12401)
                     /locus_tag="PWY43_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDL2136165.1"
                     /translation="MIYIEKIQGFAHIQDLGRYGYRHLGVSHGGAMDSLALQAGNLLL
                     KNANNAPAIELTLGGMRITFDCDTPFCLTGALVEAELDGTPVFSYYRYTANARQTLTI
                     KRIVLGNYAYLCVAGGFLVPQVLGSASTDLKAQFGGFQGRMLKAGDNIATGRRDSRLS
                     LMSIEPIDFTSHIRATASSEYEAFTADSRERFWQQGWQLQNNSNRMGYRFAEKALELT
                     ASLEMLSYSAPSGTVQVPPDGQPIVLMADAQTTGGYPKIACIIQADLGRLAQNPFGST
                     VQFEQVSREQAVEIYQKDQNYLETIRRKANESR"
     gene            complement(12398..13045)
                     /gene="pxpB"
                     /locus_tag="PWY43_001286"
     CDS             complement(12398..13045)
                     /gene="pxpB"
                     /locus_tag="PWY43_001286"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDL2136166.1"
                     /translation="MLTIHHTSEHSLLCLAEPPAELAKQQQIWRVAEQARALDGVLEV
                     VVGMNNFTLFHAIGTDSTRLIQAVQQLWENDQAQAAQSPRLIEIPVHYGGEFGEDLFE
                     VAQFHNTTPEEIIRRHTEPTYTVFMIGFQPGFPYLNGLPQNLHTPRRNVPRTKVPAGS
                     VGIGGSQTGIYPFTSPGGWQLLGRTELPLFDIQHNPPTLLTAGDNVRFVAESITL"
     gene            complement(13139..13963)
                     /locus_tag="PWY43_001287"
     CDS             complement(13139..13963)
                     /locus_tag="PWY43_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDL2136167.1"
                     /translation="MKKLLLFLLFIGNPIFAKSPELMLVGQYQDQDISGWVMSEKLDG
                     VRGFWDGQQLISRQGYPLNPPDYFIQDFPPFAIDGELFSERGKFEEISSIVRSAQPKG
                     WHKLKLQVFDVPNAQGNLIERLATLENYLKQHPTLHIAIIEQIPIKDKAHLQQFYQSV
                     LEQGGEGVIVRNPNAGYIQGRSAQILKIKPVLDEECTVVAHHKGKGKYTDKLGAITCE
                     NQRGRFRIGSGFKDKERENPPPIGSIITYKYRGVTESGKPRFATFWRIRNDQISEN"
     gene            complement(14097..14912)
                     /locus_tag="PWY43_001288"
     CDS             complement(14097..14912)
                     /locus_tag="PWY43_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136168.1"
                     /translation="MEHFIGLGVAGNFAGHLEQAGEAADFAKVEAVQPKAIFPFYVPA
                     ENLGDYQFLSTYPLSNTAINFPSDADNLQIEPEVALICEIGYQDQQVVSLTPTHFAAY
                     NDCSIRRPNANKICEKKNWGASSKGLSAIHLPLDNFNKGGTLDNYNIACFHKRNGELN
                     TYGIDSPAVEYSYFHQKLLDWIVERMNNQPDEGPMNNISEMLKQANYPTKAIISIGAT
                     RYTPFGESNLLQVGDVSMVVVYNVKKYSHSQIEEMAKLETFSEDISALIQTVR"
BASE COUNT         4135 a         3315 c         3349 g         4317 t
ORIGIN      
        1 tacgaacctg aaattccgca aaacagcgga aatattatcc gcctttgtgc caactgtggc
       61 tttcgtttgc atatgattga accgttcggc tttggttggg acgataaaaa gctccgccgt
      121 tcggggctgg attatcacga atttgtggat attcaaaaat ataaaaattt tgaagatttc
      181 cttgagcgtg ctaagcctaa acgcttgttc gccttaacca ccagagggga gcagaaccac
      241 agtgatgtga aatatgaact tggcgatttt ttaatgttcg ggccggaaag cagaggcatt
      301 ccaaagccat tattagcgga aatgccgatg tcgcaaaaaa tccgcattcc gatgtgcaaa
      361 gacagccgca gtatgaattt atctaactcg gtggcggtgg ttgtgtatga ggcgtggcga
      421 caattcggct accaaaatgc ggtagcggca caatctattt aacttgcaag cggtgtgatt
      481 ttggctattt tttgcaagtt tgtggtcaag gttgggggag gctcgacctt gactaattaa
      541 gaacatctat tgtgtctatt cttcttcatt tttagctgga ggtaaaaaat tctctccggt
      601 aacaactgat ttacctgttt tattctccaa ttcctgacgg gcatttttgg caatattgcc
      661 accttgtttt cctgcttggg cattttcatc aaccccaact gcctgcatac tttctgcaat
      721 ttgccgactg gaaagttcag caagtgctgt aaataacaat tcagcttcgc tcatatgatc
      781 tcgcagattt tggcttttta aacctttaag cgatttatgc ttttgtacag atagtccgct
      841 ccattcttga tgaatcacat tggttaaaaa tgcaaaatct tgtggctgtt caacccctga
      901 cgatttccaa taatcggtca gtttatttcg ggtttcttgt cccatcattc tttgctgaat
      961 ccatttttca cttcgtccta tcttctgcca attctccctt gctcgatcta gactttgact
     1021 tggatcgttt atttcctgta agcgttcata acctactttt gctaaccatt gtttgaaggg
     1081 ctctgctttc ggactaggga tagattgaat aagtcggaaa agttgttcgg tatctgctac
     1141 atcagtcaaa cgcatttttc cgtcagcaga gggcattttc aacccgtgac aatttgtcac
     1201 ggtttcattg ccttctgcct ttagcctctg ttttagctta cgccaataag cagagggatc
     1261 taggctttcg gttaaaatag caatcacatc aataatagaa aaataccatt tatctttttc
     1321 ttcatcgtaa tgactgcgta cagatttctg ttcaaaaagt ttaatttctt gcataataaa
     1381 aatactgtat ataaaaacaa gaaaagtata acagttgtca gcttttcagc aagtagcaaa
     1441 agtgattttt tcgatctcga tcccaaaaat gatttttggg tattgatttt tcaatcgata
     1501 aattttctgt gatcaggatc acaaaaatag cagaaaaaat agaaattgaa gttgtatttc
     1561 attaaaataa taaaaaatga agtgatttat tcagaattga tatttaagga gagactatgc
     1621 gtttaattcc gttagaaact gatcacgatg tgagtgtttg ggctgctcgt tatattgttg
     1681 atcgtatcaa tgcattccaa ccaaccaaag aaaaaccttt tgtgcttggc ttaccaaccg
     1741 gcggtacgcc tgaaaaaacc tataaagaat taattaaatt atatcaagct ggtgaagtga
     1801 gctttaaaaa tgtggtgact ttcaatatgg acgaatacgt cggcttacct aaggaacacc
     1861 ctgaaagcta ccacagtttt atgttcaagc acttttttga ccacgttgat attccgcttg
     1921 agaatatcaa tatcctcaac ggtatggcgg aagacgtgaa tgcagaatgc gaacgttatg
     1981 aagaaaaaat ccgtttttat ggtaaaatcc atctgtttat gggcggggtc ggtgtggatg
     2041 ggcatattgc gtttaatgag cctgcatctt cgctttcttc ccgcactcga attaaaacgc
     2101 ttaccgaaga tactcgcatt gcaaactcac gtttctttaa caatgacgta agccaagtgc
     2161 cgaaatttgc tttaaccgtt ggggttggta cgttgcttga tgccgaagaa gtgatgatct
     2221 tagtgaccgg ccataacaaa gccttagcat tacaggcgtg tgtggaaggg gcggtaaatc
     2281 atctttggac catcagttgc ttacaattac atcgccgtgg tatagtggtg tgtgatgagc
     2341 cggcaaccca agagctgaaa gttaaaacag ttaagtattt caaagagtta gagaaaaacg
     2401 tagctcgcta attctgattt tacaagcggt taattttgta atatttttta caaaaacgac
     2461 cgcctgttag catttaaaga gaagagagaa aatgaaccat tatgccttaa caaactgtgt
     2521 gatttacacc ggtgaacaag tgctttacca acacgcatta gtggtgaaag aagataaaat
     2581 cgactccatt gttttggaag aaaatttacc gctggatctg caaaaaatcg acctaaaagg
     2641 agcgaatctt tcagccggtt ttattgactt gcaactgaac ggctgcggtg gggtgatgtt
     2701 taatgaagaa atcactaccc gcactttaga aattatgcag gaaaccaatc tgcgttcagg
     2761 cacaaccagc tatttaccga cttttattac ctcgcctgat gagggagtga aacaagcggt
     2821 ggcggtaatg cgtgagtatt tgtcgaaaca caaaaaccaa gcactcggct tgcatttgga
     2881 aggaccttat ctaagtgtgg caaagaaagg agtacaccgt ccggaattta tccgtgaaat
     2941 cagccctgaa atgttagatt tcttatgcga taacggcgat gtaattacga aaatcacgat
     3001 tgcagcagaa aacccgaccg cagaccatat caaacgcttt aatgatgcag gaattattgt
     3061 atctatcggg cattccaatg ccacttttgc tcaaaccaaa cagcgaattg ccgaaggggc
     3121 agcgtttgct acgcatttac acaatgcaat gtcgccggtc agcaacgggc gtgaaggcgg
     3181 tgtagtaggg gcggtgttgg atagcgatat ttacaccggc attatttgcg atggcttgca
     3241 cgttgaatgg ggcaacattc gcattgccaa aaaagccaaa ggcgataaat tagtgattgt
     3301 cagcgatgct gttgctgccg caggaagtga tattgagtcg tttatttttg tcggtaaaaa
     3361 agtcacggtg aaagatggca aatgctacga tgaattcgga gcattgggcg gtgcggcaat
     3421 cacgatgatc gaatccattc gcaacgtagt gcaacattgc gatattagcc tgaatgaagc
     3481 aatccgaatg agtacccttt acccggcaaa agcgattggg gtagaggaaa cactcggctc
     3541 acttgaagcc ggcaaaattg ccaaccttgc gattttcgat aatcaatttg aagtaaaagc
     3601 gactgctgta aacgggcaat tacagtggca ttaattttgc tacaaattct aagcataaca
     3661 gcatataatc caaaggcagg cattgtgtct gccttttatt tatagggaga ataacatgca
     3721 atttaaagat tacttagcca cttttccaac tattgatcat ctagccggct taaacgtgat
     3781 aaatagcggt gaagtgattc aacatattcc tgcgattgaa ggtaaattag gttcactaaa
     3841 actttacaat gcactgtttg cgaaatttga tgggaaatta accgctagtg cagcaaaaca
     3901 agggttggaa tggtttgccg aacacgttga agatgcgaaa aataatgtgg gtaagcaccc
     3961 gaatattgat ctattattta gggtaattga gcaagattta tctttaagtt tagagccgat
     4021 tgttaaataa gattgcacta gtgaaaaata acaagcggtc attatctgca aaatttttgc
     4081 aaataatgac tgcttgtgtt taagaggctt atttcacggt aatgcgtgca aatttgcgtt
     4141 tgccgacttg gtaaacaaaa gtgccttttt gagcagtttg tttcacatct tccacttttt
     4201 caccatcaat tttaaccccg ccttgttggg ctgagcgaat tgcctctgaa gtggacggta
     4261 ctaagccggc ttctttcagt agagccgcta ggccgatttc accctcaaag gtaaattcag
     4321 gcacttcatc aggaattgcc cctttttgga aacggttgac aaactcttgc tctgctgcat
     4381 ctgctgccgc ttcatcgtgg aaacgagcga taatttcttt tgccagtaga attttcacat
     4441 cacgcgggtt tttgccattt tcaacatccg cttttaattg tgcaatttcg tctaatggac
     4501 ggaatgagag caggttgtac caatcccaca ttaaatcgtc tgaaatcgac ataattttgc
     4561 cgaacatttc gcttggagct tcagttacac caatgtagtt gccgagagat ttcgacattt
     4621 ttttctcgcc gtctaagccg acaagtagtg ggagtgtcat tgccacttgt ggtttttgac
     4681 cggctgattt ttgtaactca cgtccaacta acaagttaaa ggtttggtct gtaccaccta
     4741 attccacgtc agctttcagt tctaccgagt cgtgaccttg tagtagcgga taaataaatt
     4801 cgtggattgc gatcggttgt tggttcgtga aacggttttt gaaatcttca cgttctaaca
     4861 ttcgagcaac ggtgtagttg gaggctaagc ggatcatacc ttccgtacct aacttgccta
     4921 accattcgga gttgaatacg atcttggttt tttgtggatc taaaattttg aagatctgct
     4981 ctttataggt ttcggcattg cgtaacacgt cttcacggct aagtggcgga cgggtagagt
     5041 tttttcctga tggatcgcct accataccgg tgaaatcacc gattaagaaa tagacttcgt
     5101 gcccgaattg ttggaattga cgaagtttgt tcagcacaac agtatgacct aagtgaatat
     5161 ccggtgcagt tgggtctgcc cctaatttaa tacgcaatgg gcggttttct tttaatttgg
     5221 tgattaaatc ttcttctgaa taaatatttt ccacgccacg tttaagttcg gcaaggactg
     5281 attcgatagc ttgagtcatt tatcttcctt ttagtcttga atataaaaaa tggatctatt
     5341 atagcggtat gaattgaaat tgataggcta taggcgaaaa aaaacgccgt attttgtggg
     5401 ggaatacggc gtaaagagtt ggagtgatta cctattattt ttgaagtgat ttaatgataa
     5461 tgattatcat ctgttaagtc aatacttttt ttgaaaattt tcaataagaa ttattctcat
     5521 caattaagta agatggatac tacggcaatt agtgaagaat gtacagcgga attttcgtat
     5581 tgtaagagtg ttggtaagcg gtatttttag aaaaaacctc cgagagcggt tatttttcgg
     5641 aggttttttg caaatgagga tttacatcgt tgaaatgtat ttcaacatca cccccgctgc
     5701 aatcgcagag ccaatcacgc cggcaacgtt tgggcccatt gcgtgcatta acaagaagtt
     5761 ttgtttatcg tactcttgtc cgactttgtt tgatacccgt gcagccatcg gcacggcaga
     5821 aacacccgct gagccgataa gtgggttgat tttgtgctga ctaaagcggt tcattatttt
     5881 cgccataatc acaccgcttg cagtgccgat cccaaaggca atcacgccca atactaaaat
     5941 gcctagtgtt tgcggttgta agaatttgtc tgcgaccaat tttgccccca ccgataaccc
     6001 taagaaaatc gtcacgatat tgattaacgc attttgggcg gtgtcgttta agcgttctac
     6061 tacgccactg gttcgcagta agttgccaaa gcagaacatt cccaataacg gtgcagcatc
     6121 cggcagtaag agtgccacta ataatagcaa cacgataggg aaaataattt tctcacggtt
     6181 gcttacatta cggagctgaa ccattcggat tttacgttct tgctcggtgg ttaaggcttt
     6241 cataatcggc ggctgaatca gtggcactaa cgccatataa gaataagccg ctaccgcaat
     6301 cgcacctaat aattcagggg caagtttgct ggcaagataa atcgcggtcg ggccatctgc
     6361 cccgccgata atcccaatcg cagcggcttg tggcaatgtg aagtcgataa tgcctaacca
     6421 gtttagccct aatgcaccca gtacggtagc aaaaatccca aattgagccg cagccccaag
     6481 cattaacatt cgagggttgg caagtaaagg gccgaaatca gtcatcgccc cgacacccat
     6541 aaagataata agcggagcaa cgccgtagcc gatagcaact ttatagaata atgccaacac
     6601 accggcggta tagcccatat cgccagccat tacttcaagc tcattttgaa cagagggagc
     6661 agccagtgcg atggcatctt taatcgaatg cagatcaagg cttgtactat ttaccttagc
     6721 agcaataatt gccagttgct cggcagaacc gctgtggagt aaattgtcta atgccgtcat
     6781 tgccatacct gcttccggaa tgtttgacat caaaccacca aagccaatag gcagtagtaa
     6841 aagcggttcg aattttcggg cgatggcaag ccaaagcaac actaaactga tgacaatcat
     6901 aatcgcttgc cccagttcta aatgcattag ccccatacct ttaaaaagtg caataatact
     6961 ttccataccg gactcctagg caatactcat taaagtatca tttaccgcca caacatcgcc
     7021 ttgtttgaca ttaatgcctt gaatcgtgcc ggattttgcc gcacaaattt gggtttccat
     7081 tttcattgct tcaagaatca gtaacacatc gccttcagca acttgttgcc cctctttcac
     7141 ttccactttc aaaatgttcc ctgccattgg agcattcacc ggttcggcat tggctgatag
     7201 cgtatttgca gattttggtg cagaatcgac cgcttgcgga gccggtgttg ctggtgttgg
     7261 agcgatattg gttatatcgc caccttcact caccttcacc acaaaggctt tgccttcaag
     7321 ctcaacggta taaatggctg aaccggattg tgtttgggct tttggtgcag aagcagcgga
     7381 ggcttgtgcc ggttttgctt catcagcact tggcactggc tcaaaggcag acggattatt
     7441 gcggttttcc aaatatttcc agcccacttg ttggaagagt gccacaatca atacatcatc
     7501 aatcatgttt tcagatagcg taatgccttt ttcttttgca cttgcagtca cttctgccac
     7561 taatttttcc agttccggcg gaagatgatc ggctgggcgg tcggtaatcg gctctgctcc
     7621 ctcaagcact ctgtcttgca aggctttgtt taccggtgcc ggtgtgcgac catattcgcc
     7681 ttttaaaata cctgcagttt ctttggcgat agttttataa cgttcgccca ttaatacgtt
     7741 catcaccgct tgtgtgccga caatttgtga ggttggtgtc accaacggaa tattgcctaa
     7801 atcttcacgc acacgaggaa tttcttgtaa aaccaagtct aatttatccg acgcattttg
     7861 ttgcttgagt tggctttcga ggttggtcag catgccaccc ggcacttggg caaccaaaat
     7921 acggctatct ataccacgga gctgtccttc aaattttgca tattttttac gcacgttacg
     7981 gaagtaggca gagattttct ccagagctgg aatgctcaag ccggtttcat acggcgtgcc
     8041 ttgaagtgtg gcgacaatag actcggttgc cgggtgaccg taagtgccgg acattgatga
     8101 aatggaggta tcgacaccat cgacaccggc ttcaatcgct ttgagcaacg ccatttcagc
     8161 cataccggtg gttgaatggc aatggagatg tagctcaacg tcgtagcgtt ttttaatttc
     8221 gctgactaat tcataggctg ccattggggt taaaatgccc gacatatctt tgatcaccaa
     8281 cgaatccacg ccaatttcca ataattgctc ggttacatcc aaccacgttt gtagggtatg
     8341 aacagggctg gtggtgtaac tcaaagtacc ttgtgcgtgc ccgccgtgtt ttttgaccgc
     8401 ttgtaatgcg gcttgcatat tgcgagggtc gttgagggca tcaaatacac ggaacacact
     8461 cataccgttt gccaccgaac gctcgacaaa tttatctacc acatcgtcgg cataatgacg
     8521 ataacccaat aagttttgcc cacgtagcag catttgcatt ggggttttcg gcattgcttt
     8581 tttcagctca cgcaaacgca cccacggatc ttcgcctaaa aaacgaatac aactgtcaaa
     8641 agttgccccg ccccaagttt ctaacgacca atagccgata ttatccagct cggaggcaat
     8701 cggtaacata tcgtctaagc gtaaacgagt cgcaaaaagg gattggtgtg catcacgcaa
     8761 aaccacatcg gtaatcgcaa tttttttcgg ttgagttgtc ataatcaaat tccttctaat
     8821 tattttattt ttgaaatctt gttaatgttg cccgttaatt cgacgatgat gagcaatcgc
     8881 agcggcaatc acagggcgta aacgctctaa atcatctggg ggagattgag gcacagtttt
     8941 agaggctgga atagaaacag gttcggggaa atattgattg ataatcttgg aaataaagct
     9001 gatggcataa attaaaacca gcaggaacag cataacaaat cccattccgg ctaccattaa
     9061 atttatacct tccgcaaaga gttctgcagg tgccataatt tatccttaaa atgtagaaat
     9121 gatgaagaaa atgtagaaaa aattcagatt tatttcaaat tatttagcct caaaatgagt
     9181 ggtagatcac aaactttaaa cgcaagcggt cagattttac aaatttttta caagagtagt
     9241 taaaatttgc aaaacctgac cttaggttag cttgaagcta agtcgttatg ccattagccc
     9301 cgaaatatat tttgaaaggg tcattgcact tagcaccgcc attagaatca ccacaatcgc
     9361 ccctgaaacg gtcatccaca cagggtgttt gtagtcgccc acaatcttgg ttttgtgagc
     9421 tgccagcaga attaacccga gggaaatcgg tagaattaag ccgttaagcg tccctacaaa
     9481 caccaagact tgtgccggtc tgccgatggt tgccagcacg gcggttgaaa ccacaataaa
     9541 ggcgacaatg aaacagtttt tgtgtttttc gataaatggc gagaatgaag taatgaagga
     9601 aaccgaggta taagccgccc caatcacaga ggtaatggag gctgcccaaa tcaccacgcc
     9661 gaagatcatc aaaccgacat cgccggcaac gtgggcaaat ggggtagccg cagggttttt
     9721 cggatcaagt tgcacgccca ttgacaccac gcccagcacc gctaaaaaca gtaccacacg
     9781 cataattgag gcgattaaaa tggctgacac cgagcttttg ctcacctctt ttaaattctc
     9841 tttgccttta atgcctgcat caagcaaacg gtgcgcacca gcaaaggtga tgtatccacc
     9901 aacagttccg cccactagcg tgacaatcgc aatcacgtct aatttttccg gcacaaaggt
     9961 atgcaccacc gcatcgtcga ttggcggatt ggcttttacg gcaacgaaga tggttaaagc
    10021 aatcatcaca aagcccataa tttgtgcaaa tctgtccatc actttaccag cttcacggaa
    10081 caggaacacg cccacggcaa tcgcaccgct gaccaccgca cccatttcag gggaaagtcc
    10141 ggtgagaatg tttagcccta aacctgcacc gcccacgttg ccgatattaa aggctaaacc
    10201 gcccatcacg ataagcagag ccagaaagta acctgccccc ggcaatacgg cattggcgat
    10261 gtcttgagct ttcttttccg atactgctac aattcgccaa atattcagtt gagcaccgat
    10321 atcaagcaag atcgagagta agatcacaaa gccgaagctg gcaagtaggg tttgggtgaa
    10381 ggtggctgtt tgggttaaga accccggccc gatggcggag gtcgccatta aaaatgctgc
    10441 cccgagtagg gcgctacggt tgttgtttga cattgtgttg gttccttttt tgttgttaat
    10501 gagtcattat tcgtggattt atcatggtaa tattcccctc tttagcaaag aggggttagg
    10561 ggagatttgg cagtagaact catcgaagtg agagagttaa taatgctcaa tttaacctct
    10621 tgaaattact tattttctcc gtaaatcgct tctgtcaaat ctcccccagc ccctcttttc
    10681 taaagagggg agttcgatct ccaagagttt tatttctggt tttccgtcca ttttgactaa
    10741 aacaggatca ggctttcacc aaaatcccct ttgcctgtaa ctctttttta attcgctcgg
    10801 caaataccac ggcgtgttcg ctgtcaccgt gtaagcaaat gctgtcggct ttaatcgcca
    10861 cattaccacc atcaacggtt ggtacagtgc cgtctaacac catttgcaag acttggctga
    10921 tcgcttcatc atcgctttct accattgcat catcacgggt gcgaggcacg agcgagccgt
    10981 cagctaaata gtggcggtcg gcaaagactt cggaaatggt ttccaaaccg tgctgctcgg
    11041 caatgctcaa catcaggctg ccggataagc ccatcagttt gaggttggga tcgaactgtt
    11101 tgaccgtatt ggcgataagg gtggcaaggt gagggtcttt ggcggccata ttgtataaag
    11161 cgccgtgcgg tttaacgtag ctcatcggca tattttccgc atcacataag gctttggcgg
    11221 ctcccagttg gtagagcaga caggctttaa gttcatcatc gggcagttgc atattggtac
    11281 gcccaaagtt ttccctatct ggaaaactcg ggtgagcgcc aactcgcacg ctgttttcct
    11341 tcgcccagtt aatcgcttta cgcatttcat tgtaatcacc ggcgtgcaag ccacagcaga
    11401 cattcgccga agtaacgagc tgtaataatg aatgatcgtt gccacaacct tcggcaagat
    11461 cgacatttaa atcaacgtga ttcattcgct tttctccgaa ttgtttccaa ataattttga
    11521 tctttttgat aaatttcgac cgcttgttca cggcttactt gctcaaattg caccgttgag
    11581 ccgaacggat tttgtgccaa ccgcccgaga tcggcctgaa taatgcaggc aattttcgga
    11641 taaccaccgg tggtttgggc atctgccatc agcacaatcg gctggccgtc cggcggcact
    11701 tgcaccgtac cactcggggc tgagtaggag agcatttcaa gggaagcggt caattccaac
    11761 gctttttctg caaaacggta gcccatacgg ttgctgttgt tttgcagttg ccacccttgt
    11821 tgccaaaagc gttcacggct atctgcggta aaggcttcat attcgctcga agcggttgcc
    11881 cgaatgtgtg aggtgaaatc aatcggctca atcgacatca aagaaagtcg gctgtctctg
    11941 cggcctgtgg cgatgttatc gccggctttt aacattctgc cttgaaaacc gccaaattga
    12001 gctttgagat cggtacttgc cgaacctaac acctgcggca ccagaaaacc gcctgccacg
    12061 cataaatagg cgtaattgcc gagaacaatc cgttttatcg tcagcgtttg acgggcgttg
    12121 gcagtgtagc ggtaatagga aaaaaccggc gtgccgtcca gctccgcctc caccaatgca
    12181 ccggtgaggc aaaatggcgt gtcgcaatca aaggtaatcc gcattccgcc aagggtaagt
    12241 tcaatcgcag gtgcattatt tgcatttttt aacaataaat tacccgcttg taaggcaagg
    12301 ctgtccatcg caccgccgtg gctcacgcct aaatggcgat aaccataacg ccctaaatct
    12361 tgaatgtggg caaaaccctg tattttttcg atataaatca tagcgttata ctctccgcca
    12421 caaatctaac attatcgccg gctgtaagta gcgttggcgg attgtgttga atatcaaaca
    12481 acggtaactc ggttcgtcct aacagttgcc agccacctgg tgaggtaaag ggataaatgc
    12541 ctgtttgact accgccgatg ccgaccgaac cggcagggac tttggtgcga ggcacgtttc
    12601 ggcgtggggt gtgtaaattt tgcggtaagc cgttgaggta aggaaagccg ggttgaaagc
    12661 cgatcataaa cacggtgtaa gtcggttcgg tatgacggcg gataatctct tcgggagtgg
    12721 tattgtggaa ttgggcgact tcaaacagat cttcgccaaa ttcaccgccg taatggacag
    12781 ggatttcaat caggcgtggg gattgtgcgg cttgggcttg gtcgttttcc cagagttgtt
    12841 gaaccgcttg gataaggcgt gtgctatcgg tgccaatggc gtgaaatagg gtaaaattgt
    12901 tcattcctac caccacttcc aacacgccat ctaaggctct tgcctgttct gccacccgcc
    12961 aaatctgttg ctgtttggca agctctgcag gcggttcggc taagcagagc agggaatgtt
    13021 cgctggtatg atgaatcgtc agcataattg ccctcaaatt gttacaaaaa ggtaacaact
    13081 ttttacgctg atatatccag actgtcaatt ttattttgtt gaaaatgtga aatagctctt
    13141 aattttcaga aatttgatca tttcttattc gccaaaacgt tgcaaatctg ggcttgcctg
    13201 attctgtgac acctcgatat ttataggtaa tgattgagcc aatcggtggc ggattttctc
    13261 gttctttatc tttaaaaccg ctgccaatcc gaaaacgtcc acgttgattt tcacaagtga
    13321 ttgccccgag tttatcggta tatttccctt tgcctttatg atgggcaacc acagtgcatt
    13381 cctcatctaa aaccggtttg attttcaaaa tttgggcaga acgaccttga atatagcctg
    13441 cattcgggtt gcgaacgata accccttcgc caccttgttc cagcacagat tggtaaaatt
    13501 gttgaaggtg ggctttatct ttaatcggaa tttgctcaat aatagcgata tggagcgttg
    13561 gatgttgctt cagataattc tctaaggtcg ccagccgttc aatcaaattc ccttgtgcat
    13621 ttggcacatc gaacacctgt aatttcagct tgtgccaacc tttgggctga gcggaacgca
    13681 caatgcttga aatttcctca aatttgcccc gttcgctaaa cagttcgccg tcaatcgcaa
    13741 aaggagggaa atcttgaata aaatagtcgg gtggattgag cggataacct tggcggctga
    13801 tgagctgttg accgtcccaa aaacctcgca cgccatcgag tttttctgac attacccagc
    13861 cactgatgtc ttgatcttga tattgcccaa cgagcattaa ctcgggcgat ttggcaaaaa
    13921 tagggttacc aatgaagagt aggaataaga gcagtttttt catagtctct ccgaaatagt
    13981 gcctccgaat atgcaaggca ctataaagga aagcgtgcaa acagtcagaa atgaatctca
    14041 atttttcgac gcagatcgca aaattttccg aaaaactgat cgcttgagtg agaaatttaa
    14101 cgtactgttt gaattaaagc agaaatatcc tcgctaaagg tttccaactt cgccatttct
    14161 tcaatttggc tatgagaata tttcttcaca ttgtaaacga ccaccatact tacatcacct
    14221 acttgcaata aattactttc gccaaaaggc gtgtagcgtg ttgccccaat gctgataatc
    14281 gctttagttg ggtagtttgc ctgttttaac atttcggaaa tgttgttcat cggaccttca
    14341 tcgggctgat tattcattct ctctacaatc caatcgagca atttttgatg gaaatagctg
    14401 tactccaccg cagggctgtc gatgccgtag gtattcagct cgccattacg tttatggaaa
    14461 caagcgatgt tgtaattatc cagcgtgccg cctttgttga agttatctaa tggaagatga
    14521 attgccgaaa gccctttaga cgaagccccc cagtttttct tttcacagat tttattcgca
    14581 ttcggacggc ggatagagca gtcattgtaa gccgcaaaat gggttggcgt gagcgaaacc
    14641 acttgctgat cttggtagcc aatttcacaa attaacgcca cttcaggctc aatttgcaga
    14701 ttatcagcat cactcgggaa attaatcgca gtatttgaaa gcggataggt ggataaaaac
    14761 tgataatccc ccaaattttc cgccggcaca taaaacggga aaatcgcctt tggctgcact
    14821 gcttccactt tcgcaaaatc cgccgcctct ccggcttgtt ctaaatgtcc agcaaaattg
    14881 ccggccacgc ctaaaccgat aaagtgttcc ataatctact ccttaattgg taaaagttgg
    14941 ataaaatttt accgcttgta accaaaaatt gcgaatgttt attttgatct gattgacaga
    15001 aaattattga attgagatta aatttagcca tattttgaac ataaaattag cgctttgtta
    15061 taaaaacaag caagcggtca ttttttttca aattttttac aaaaaccgct tgcgtt
//
LOCUS       DANKBV010000052        14957 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.052, whole genome shotgun sequence.
ACCESSION   DANKBV010000052 DANKBV010000000
VERSION     DANKBV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14957
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.052"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..167)
                     /locus_tag="PWY43_001289"
     CDS             complement(<1..167)
                     /locus_tag="PWY43_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2136169.1"
                     /translation="MKAQQLKNAILQLAIQGKLVPQDPNDEPASELLCKIQAEKDRLI
                     AEGKIKKNKKTA"
     gene            complement(373..2034)
                     /locus_tag="PWY43_001290"
     CDS             complement(373..2034)
                     /locus_tag="PWY43_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL2136170.1"
                     /translation="MLTWEHIEKMIAHQQFIREKAEIEYKEAKNSVPKDLWHTYSAFA
                     NSNGGFILLGIKENEQGHFPELLISGVNDPDKVIDELFSQSRSEKVSKSLLKDNDVKI
                     FQVENKSVIAIYVKAADILDRPIHLNGDYRLSYVRLNSGDHKLTSSELRSFISSYSQK
                     DADGRIVPYASIDDISLSTLDKYRNLLKAHNSTSKLLTLSDEKLLQEIGAYKKDLAQN
                     SEGITNAGLLMFGKHYAITSIFPHFFFEYYEEAGDVKRYTLRITDFDLEEGNLFEFYL
                     KIVPLIQELGKDKHFKLDNLTRTEENEITDGLREALINAITHADYFNNVRHLKVIKTK
                     DNKLIFENAGVMLVDIPTAIAGQRSECRNSIIHNLFHRLGLCERQGQGVRDIYSYWQS
                     RNFNSPLLESEIDYTKLTLTFQSGMIADFEEKFTACFGKNFSELQPIQKDCLLFIAFN
                     QGKAMFAELAQKLPQYPRRNITLALARLKEKGLLTSSGSPRKQLYTLVNLEWIITEKS
                     QDDYFNPPFDQLDKASESKGAPPKKSKVKQVKQLPQNKANKLPPK"
     gene            complement(2047..3516)
                     /locus_tag="PWY43_001291"
     CDS             complement(2047..3516)
                     /locus_tag="PWY43_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL2136171.1"
                     /translation="MSLNNIVKRLQDVMRNDAGINGDAQRIEQIVWILFLKIYDAKEQ
                     EWELENDEYHSILPNFLRWQNWAEDKKDGKAMTGDELLSFVNNELFPTLKNLPISADT
                     PMNQRIIRAAFEDNNNYMKNGVLLRQVINIIDEIDFAHYQERHAFGDIYENILKSLQS
                     AGNAGEFYTPRAVTDFMVQAIKPKLGERIADFACGTGGFLTSALKVLESQIQTLSDRT
                     LFNNSVYGIEKKALPHLLCITNLLLHDIDNPNVHHDNALEKSVKDYTENDKFDVILMN
                     PPYGGSEIEQIKKNFPTALQSSETADLFMSVIMYRLKQNGRAAVVLPDGFLFGTDNAK
                     VAIKKKLLEEFNLHTIIRLPHSVFAPYTSITTNILFFDKTEPTRETWVYRLDMPQGYK
                     NFSKTKPMKLEHFNEVVEWWGNRQVIEVEGFDKAKSFTLKEISDRQYNLDLCGFPHEE
                     EEILPPDELIADYQRKRTAYNADIDKILAEITDILGIKL"
     gene            complement(3564..5915)
                     /locus_tag="PWY43_001292"
     CDS             complement(3564..5915)
                     /locus_tag="PWY43_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2136172.1"
                     /translation="MYTLQELKQMNEQEIRSRLISPAIRNAGWSDRQIGEEYTFKTNK
                     RFTDGQVVVDPKTTQTKRIEAKRVDYLLYTSANQKIAVVEAKDNHHSSRHGLQQAMTY
                     ARLLDVPFAYSSNGDEFVEHDFITGVQRTLPMSAFPTPDELHQRWAKQYTTEELKIIQ
                     QPYYTSSTDTFKEPRYYQEIAINRTVEAVAKGQQRILLVMATGTGKTYTAFQIIHRLK
                     TSGAKKKILFLADRNVLVDQPMMQDFKPFKDKMVKVSNKKLDSAYEVYLALYQQLDGE
                     TDNELFKQFQPSFFDLIVIDECHRGSAKEDSDWRAILKYFKDATHIGMTATPKETDDA
                     SNITYFGEPIYTYSLKQGIEDGFLAPYRVIRVDLDCDLKGWRPTKGLKDHQGNEIEDR
                     LYTVKDFDYNIVLNDRTRLVAERICEFLRKTDRLQKTIVFCANINHAERMVAELSRLN
                     ADMLKHNSKYVCQITGDQSGKGVDLEEFCDVANEGKDYPVIAVTSKLMTTGVDSKTCK
                     LVVLDANIQSMTEFKQIIGRGTRLRPDFGKEFFTILDFRGVTRLFADPAFDGEPVVIL
                     DVPENKPLVEQPLPKPSDKDIIKDPELKPYKIPVVVDFGTLKLRILDEKVQYLNEQGE
                     LVSEDIDLFSKREMQKEFGSYEQFQQQWTTSGEIFSKFYTDPKWLAALRQTRQFSHDV
                     EDFDVLSHISFGKKPLTKTERAEKVKQSGYVEQYSPENQQVLGLLLNEYVKSGNQDLT
                     KTEILKTPQFSRFGGLVSIIKNFGGKELYQKAVKGLEKMIYSE"
     gene            complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PWY43_001293"
     CDS             complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PWY43_001293"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251643.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL2136173.1"
                     /translation="MQLKKYPVPTYFQRVKIALHYLLPQLAITRAAGWLAEQKWGVVT
                     HLIIKLFAKQYKVNLSEAKKTEPSDYATFNEFFIRELKENARPINQDPNTLCLPADGK
                     VSESGEISDNRLLQAKGHHFTLETLLANDEEMAAKFKDGTFITTYLSPTDYHRVHMPC
                     DGTLRKMIYVPGELFSVNPFLAEHVPNLFARNERVICEFDTAFGPMLQILVGATVTAS
                     MSTVWAGVINPPRADEVVVWNYETEGEKAIKLQKGEEMGAFRLGSTVINLFPKGKVHL
                     NPALIAGMQTRMGEELGRIIN"
     gene            complement(7045..7383)
                     /locus_tag="PWY43_001294"
     CDS             complement(7045..7383)
                     /locus_tag="PWY43_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDL2136174.1"
                     /translation="MKKIKQLGLTFLFGLNAMAFAQTPPTTMPVEQNAVTQPVQQTQI
                     TNANAVNINTASAAELQDKLVGIGQKKAQAIVDYRTKNGNFKNIEQLAEVSGIGQATV
                     EKNRANIILE"
     gene            7676..8950
                     /locus_tag="PWY43_001295"
     CDS             7676..8950
                     /locus_tag="PWY43_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="opacity-associated protein A"
                     /protein_id="HDL2136175.1"
                     /translation="MTQNNFRKEPVFGESSVTSQDAEATKAPGHTFTPIMKRPDAKVE
                     DHTADVFSAKQAKEFQFTPSSEATEAVEPDNTIHSVEESVSTASFNTVEAQENVEKEV
                     VKPAPESFTTERIIPNAATAAAAATAAAAATKTKEIKAKMPPKTRRLLLVALLALALL
                     VLFFLLKPSTPETVEELQSQQGGSLPIEFRPVDEAEAKHAEEQARAEQEAAEAAKAQE
                     QAQAAQQESVTVAPAQTEQTMAKPSQDSQSQTQSQAVVEQPVAVKPVENKPVVAQPAI
                     KPKTENSVVFQPETTVKQQPKAPAKTEVKTERATLAAKETAKTAAKPVEKAPTKTEAV
                     KAAPAAESANAVATKTMTVPKDVSLMQVFRDNNLNISDVNAMSKVNNVVSNLKVGERV
                     TVRLDKNNRVVEMSIGSGGKFTRQANGSYTFK"
     gene            9064..9426
                     /locus_tag="PWY43_001296"
     CDS             9064..9426
                     /locus_tag="PWY43_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HDL2136176.1"
                     /translation="MLVACSTVVTPVEQIHQVQKEKEPVKKVTKLIAQAPKVSLYRCE
                     NDKKVEVERQGRTKKALKKEIVTVSYQGTTHQLSPSVTRDGKKYTNIRWTWHETRSGK
                     AFLYNNTKKTLAANCIKQ"
     gene            9537..10571
                     /gene="waaF"
                     /locus_tag="PWY43_001297"
     CDS             9537..10571
                     /gene="waaF"
                     /locus_tag="PWY43_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HDL2136177.1"
                     /translation="MNILVIGPSWVGDMMMSHALYQQLKIQYPNCQIDVMAPDWCRPL
                     LARMPEVRNAISMPIGHGSFRLCERYRLGKSLRNQYDMAIVLPNSLKSAFIPLFAKIA
                     RRRGWKGESRYFFLNDLRSNKNDYPMMVQRYVALAFEKATVPTAQQLPIAYPYLTTDS
                     EQIEQTKAKFEKQLAYAENRPAIGFCPGAEFGPAKRWPHYHYATLAKMLIEQGYSVRL
                     FGSKKDEAAGEQIRLALPENLQRYCLNLAGQTDLNQAVDLIADCNAVVSNDSGLMHIA
                     AALNKPLVALYGPTSPQYTPPLSQNAVIIRLIEGGLIKIRKEGAEGYHQSLIDIQPEM
                     VMDKLAELLI"
     gene            10639..11598
                     /gene="rfaC"
                     /locus_tag="PWY43_001298"
     CDS             10639..11598
                     /gene="rfaC"
                     /locus_tag="PWY43_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDL2136178.1"
                     /translation="MKILIVKTSSMGDVLHTLPAITDMQNAIPDLKVDWVVEENFTEI
                     PAWHSAVNKVIPVAIRRWRKNLCQRQTWVEWQQFLKQLKSEQYDAVIDAQGLIKSAVL
                     VTRQAKGKKFGYDKNSAREGLSSLFYDETFNIPYQQHAVERIRKLCAKSLGYALPTER
                     GDYCIAQHFAKNPQKTTAYIMLIHATTRADKHWQEAYWAELIQALDKHNLAVRLPWGN
                     QAEKERAERLASVANNAKVLPKMSLTDIATQLAGAAAVVSVDTGLSHLAAALDKPNVI
                     LYGATDPKLIGAYGQNQFYLKADKMENITSSQVLEKLLPLINK"
     gene            11719..12093
                     /gene="rpsL"
                     /locus_tag="PWY43_001299"
     CDS             11719..12093
                     /gene="rpsL"
                     /locus_tag="PWY43_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL2136179.1"
                     /translation="MATINQLVRKPRVKKVVKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRIRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVKDRKQGRSKYGVKRPKA"
     gene            12224..12694
                     /gene="rpsG"
                     /locus_tag="PWY43_001300"
     CDS             12224..12694
                     /gene="rpsG"
                     /locus_tag="PWY43_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL2136180.1"
                     /translation="MPRRRSIEPRKILPDPKFGSELLAKFINVLMVDGKKSTAEKIVY
                     GALETLAQRSGKEALEAFEIALENVRPTVEVKSRRVGGSTYQVPVEVRPTRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAADNKGSAVKKREDVHRMAEANKAFAHFRW"
     gene            12796..14898
                     /gene="fusA"
                     /locus_tag="PWY43_001301"
     CDS             12796..14898
                     /gene="fusA"
                     /locus_tag="PWY43_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2136181.1"
                     /translation="MARTTPISLYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMSQQYPQHRINVIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRTGANFLRVVEQIKT
                     RLGGNSVALQLPIGAEDSFKGVVDLIKMKAINWNEADQGMTFTYEDIPADMLEACEEH
                     RAMLVEAAAEASEELMEKFFSGEELTEDEIKAALRQRVLAGEVIPVCCGSAFKNKGVQ
                     AMLDAVIDYLPAPTDIPAIKGINEDETEGERHASDDEPFASLAFKIATDPFVGNLTFF
                     RVYSGVINSGDTVYNSVKQKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTG
                     DTLCAIDAPIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEE
                     SGETIISGMGELHLDIIVDRMKREFKVEANIGKPQVSYRETIRTRVNDVEGKHAKQSG
                     GRGQYGHVVIDLYPLDAEGPGYEFVNEIKGGVIPGEYIPAVDKGIQEQLKSGPLAGYP
                     VVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFAKASPVLLEPIMKVEVETPPDYV
                     GDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLK
                     YAEAPKNVADAIIEARRK"
BASE COUNT         4424 a         2904 c         3152 g         4477 t
ORIGIN      
        1 gcagtttttt tgtttttttt gatctttcct tctgcaatca agcggtcttt ttctgcttgg
       61 attttgcaaa ggagttcgct tgccggttca tcatttggat cttgtggaac gagcttgcct
      121 tggattgcta gttgtaggat tgcgtttttt agctgttggg ctttcatttt ttattctcaa
      181 taatcggttg ttatttctta aagtatcttt gattacggtg ttttgggttt tctgggaatt
      241 gcatttcgat cacaccaagc gttagtgcag gttttaaata ctgcatacgg aaatgcttat
      301 catcttttaa cctgagtttt ttcataattt ctttgctctt catcggctca agcaacactg
      361 aaagtaagcg atttacttcg ggggtaattt gtttgctttg ttctggggta attgtttgac
      421 ttgcttcact ttgcttttct tcgggggggc gcccttactc tcactagctt tatcaagttg
      481 gtcgaaaggt ggattaaaat aatcatcttg tgatttctct gtgattatcc attctaaatt
      541 cactagggta tagagttgtt ttctcggact ccccgaactg gttagtaagc ctttttcttt
      601 cagtcgtgct agtgccaaag taatatttct gcgtggatat tggggcagtt tttgtgctaa
      661 ttctgcaaac attgctttac cttgattgaa ggcgataaag agtaagcaat ctttttgaat
      721 aggctgtaac tcagagaaat ttttgccaaa gcaagcggta aatttttcct caaaatctgc
      781 aatcatacct gattgaaaag ttaaggtgag tttagtgtaa tcaatttcac tttctaaaag
      841 cggtgaattg aaatttcggc tttgccaata tgaataaata tcccgaacgc cttgtccttg
      901 tcgctcacat agccctaaac ggtggaataa gttatgaata atagaattac ggcattccga
      961 tctttgccct gcaatagccg tgggaatatc aaccaacatt actcctgcat tttcaaaaat
     1021 cagtttgtta tctttggttt taatgacttt tagatggcga acattgttaa agtaatctgc
     1081 gtgagtaatt gcattgatga gagcttcccg tagcccgtca gtaatttcat tttcctcagt
     1141 acgggttaaa ttatctaatt tgaaatgttt atctttacca agctcttgaa taagtggcac
     1201 aattttcaag taaaattcaa agagattgcc ttcttcaaga tcaaaatcgg taatccgtaa
     1261 tgtatagcgt tttacatcac cagcttcttc gtaatattca aaaaagaagt ggggaaatat
     1321 gcttgtaatg gcatagtgct taccaaacat caataagcct gcattggtaa tgccctcgct
     1381 attctgtgct aaatcttttt tataagcccc aatttcttgc agcaatttct catcggataa
     1441 cgtaagtaat tttgatgtgc tgttatgggc tttcagcaga tttctgtatt tatccaatgt
     1501 gcttaaactg atatcatcaa tagaagcata aggcacaatt ctcccatcag catctttttg
     1561 gctatagctt gaaatgaaag aacgcagttc agaggaggtg agtttatgat cgcctgaatt
     1621 taaacgaaca taagaaagtc gataatcacc atttaaatgg ataggacggt ctaaaatatc
     1681 agctgctttc acataaattg caatcacgct tttattttca acttgaaaga ttttcacatc
     1741 attatccttt aataaggatt tactgacttt ttcactacga gattgggaaa ataattcatc
     1801 aatgacttta tcaggatcat taacaccaga aattagcagt tcaggaaaat gtccttgttc
     1861 attttccttt attcctaata aaataaagcc accattgctg tttgcaaaag ccgaataagt
     1921 atgccaaaga tcttttggca cactattttt tgcctcttta tattcaattt ctgccttttc
     1981 tcggataaat tgctgatgtg caatcatttt ttcgatatgt tcccaagtca gcattttatg
     2041 ctcctattac agtttaatgc ctaaaatatc ggtaatttct gctagaatct tatcaatatc
     2101 agcattgtaa gcggtgcgtt ttcgctgata atctgcaatt agttcatcag gcggaaggat
     2161 ttcctcttct tcgtgaggaa agccacataa atcgaggttg tattgacggt cggaaatttc
     2221 ctttaatgta aaacttttcg ctttgtcaaa cccttcgact tcaatcactt ggcgatttcc
     2281 ccaccattca acaacttcgt tgaaatgttc caatttcatc ggtttagttt tggagaaatt
     2341 tttataaccc tgtggcatat caaggcgata aacccaagtt tcacgggttg gctcggtttt
     2401 atcaaaaaac agaatattag tcgtaatcga ggtataaggg gcaaagacac tatgcggtaa
     2461 gcggataatc gtatgcagat taaactcttc taatagcttt ttcttaatcg caactttggc
     2521 attatctgtg ccgaacaaaa atccgtcagg cagcaccacc gcagcccgtc cattctgttt
     2581 taggcggtac ataattaccg acataaacag atcagcggtt tcactgctct gtaaagccgt
     2641 agggaaattc tttttaattt gctcaatttc tgaacctccg taaggtggat tcatcaaaat
     2701 cacatcaaat ttatcatttt cggtgtaatc tttgaccgat ttttctagag cattatcgtg
     2761 atgtacattc ggattatcaa tatcgtgcaa caataaattg gtaatacaaa gcaggtgagg
     2821 aagggctttt ttctcaatgc catacacaga attattgaat aaagtgcggt cagaaagggt
     2881 ttgaatttga ctttccaata cctttaacgc cgaagttaaa aaaccacccg tcccacaggc
     2941 aaaatcggca atgcgttcgc caagtttcgg tttgattgct tgtaccataa aatcggttac
     3001 agcgcgaggg gtataaaatt cccctgcatt acctgcactt tgtaaacttt tgaggatatt
     3061 ttcataaata tcgccaaagg cgtgtcgttc ttggtaatgg gcaaaatcaa tttcatcaat
     3121 gatattgata acctgacgta ataacacccc atttttcata tagttgttat tatcttcaaa
     3181 agcagcacgg ataatccgtt ggttcatcgg cgtatcggct gaaataggca gattttttaa
     3241 agtcgggaaa agttcgttat tgacgaaaga gagtaattca tcaccagtca tcgctttgcc
     3301 gtctttttta tcttccgccc aattttgcca gcgtaaaaaa ttcggcaaaa ttgagtgata
     3361 ttcatcgttt tccagttccc actcttgttc tttagcatcg taaattttca agaataagat
     3421 ccagacgatc tgttcaatgc gttgggcatc accgttaatc cctgcatcgt tacgcattac
     3481 atcttgcagg cgttttacta tgttgttgag gctcattgtg atccttgttt atctgtaaaa
     3541 ttttgttgta aaataaccgc ttgttattcc gaataaatca tcttttctaa gccttttacc
     3601 gctttctggt agagttcttt accgccgaaa tttttaataa tagacactag acctccaaaa
     3661 cggctgaatt gcggtgtctt taaaatttcg gttttagtca gatcttgatt gccggatttg
     3721 acatattcat taagaagcaa ccctaaaact tgctggtttt cgggcgaata ctgctccaca
     3781 tagccacttt gtttcacttt ttctgctcgt tcggttttag tcagtggttt tttgccaaag
     3841 gagatgtggc ttaatacatc aaagtcttca acatcgtggc tgaattggcg tgtttggcga
     3901 agggctgcca gccattttgg atcggtgtaa aatttcgaga aaatttcacc gcttgttgtc
     3961 cattgctgtt ggaactgttc gtaactgccg aactcttttt gcatttcccg ttttgagaac
     4021 agatcaatat cttcagaaac cagctcgccc tgctcattca gatattggac tttctcgtct
     4081 aaaatgcgta atttgagtgt gccgaaatcg acaactaccg ggattttata tggctttagc
     4141 tcggggtctt taatgatgtc cttatcactg ggtttaggaa gcggttgttc gacaagtggc
     4201 ttattttccg gtacatctaa aatcaccacc ggctcaccat caaatgccgg atcagcaaac
     4261 aggcgagtaa cgcctcgaaa atctaaaatg gtgaaaaatt ctttgccgaa atcagggcga
     4321 agacgagtgc ctcgtccgat aatttgttta aattccgtca ttgattgaat gtttgcatca
     4381 agtacaacca gcttacaggt tttgctatct acacctgttg tcatcaattt agaggttacg
     4441 gcaatcacag ggtaatcttt cccttcattt gctacatcac aaaattcttc taaatcaacg
     4501 cctttgcctg attggtcgcc tgtaatttgg cagacatatt tggaattgtg tttcaacata
     4561 tcggcattga ggcgtgaaag ctctgccacc attcgctctg catggttgat gttggcacag
     4621 aagactattg ttttctgcaa gcggtcggtt ttgcgtaaaa attcgcaaat tctctccgca
     4681 accaagcggg tgcgatcatt tagcacaatg ttgtaatcga aatctttgac tgtatagagg
     4741 cgatcttcaa tctcattacc ttgatgatct tttaaacctt tggttggtcg ccagcctttt
     4801 aaatcacagt ccagatcaac acgaatcact cgataagggg caagaaagcc gtcttcaata
     4861 ccttgtttga ggctataggt ataaatcggc tcgccaaaat aggtaatgtt gctggcatca
     4921 tcggtttctt tgggggttgc agtcataccg atatgggtgg cgtccttgaa atacttcaaa
     4981 atagcacgcc agtcagaatc ttcttttgct gaaccacgat gacattcatc gatcacaata
     5041 agatcaaaga aagagggttg gaactgctta aacagttcgt tatcggtttc accgtcaagc
     5101 tgttgataaa gtgcaagata gacttcataa gcactgtcga gctttttatt gctgactttc
     5161 accattttgt ctttaaacgg tttgaagtct tgcatcattg gctgatcgac tagtacattg
     5221 cgatcggcaa gaaataaaat tttctttttc gcaccactgg tttttaaacg atggataatt
     5281 tggaaagccg tataggtttt acccgtgcct gttgccatta ccaataaaat cctctgctga
     5341 ccttttgcta cagcttcaac agtacggtta atcgcaattt cttggtagta acgaggctct
     5401 ttaaaggtgt cagtgctact ggtgtaataa ggctgttgaa taattttcag ctcttcggtg
     5461 gtatattgtt tcgcccaacg ttggtggagt tcgtcgggag taggaaatgc tgacatcgga
     5521 agggttcgtt gtacgccagt gataaaatcg tgttccacaa actcatcacc atttgagcta
     5581 tatgcaaaag ggacatcaag caatcgagcg tatgtcattg cttgctgtaa gccgtgtcgg
     5641 gaagagtgat ggttatcttt ggcctcgacc acggcaattt tttgatttgc ggaagtatag
     5701 agcagataat caacccgttt tgcttcgatg cgtttagttt gcgtcgtttt tggatcaaca
     5761 actacttgcc catctgtaaa tcgtttattg gttttaaatg tgtactcttc gccaatttgt
     5821 ctatccgacc atcctgcatt tctaattgca ggagagatta atcgagagcg aatttcttgc
     5881 tcgttcatct gctttaattc ttgaagtgtg tacatatttt tccttccgat aagagtttct
     5941 tatcggaaga ttatagcaaa cagtttaggt aaaattaact caggatttaa ttgataattc
     6001 tacccaactc ttcccccatt cgggtttgca ttcctgcaat aagtgcaggg ttgagatgta
     6061 ctttaccttt cgggaagagg ttaattacgg ttgagcctaa acggaaagct cccatttctt
     6121 cgcctttttg gagtttgatg gctttttcgc cttcggtttc gtaattccaa actaccactt
     6181 catcagctcg aggcggatta attacgccag cccaaaccgt actcatactt gcggtaaccg
     6241 ttgcaccgac caaaatctgt agcattggtc cgaatgcggt gtcaaattca caaatcacac
     6301 gttcattgcg agcgaataag tttggaacgt gttcggctaa aaacggattg acggagaaca
     6361 attcgcccgg cacataaatc attttacgca atgttccatc gcacggcata tgtacacgat
     6421 ggtaatcagt tggcgataag taggtggtaa taaatgtgcc gtctttaaat tttgccgcca
     6481 tttcttcatc atttgccaat aaggtttcta gcgtaaaatg atgccctttt gcttgtaata
     6541 aacggttatc cgaaatttcg cctgattcgc tgactttacc gtctgccggt aagcaaagtg
     6601 tatttggatc ttgattaatc gggcgtgcgt tttcttttaa ttcacggata aaaaattcat
     6661 taaaagtagc ataatctgac ggctcggttt ttttagcttc agataaattg actttatact
     6721 gttttgcaaa cagtttgatg ataaggtgag taactacgcc ccatttttgc tctgctaacc
     6781 aacctgccgc acgggtaatt gccagttgtg gcagtaaata gtgcagagcg attttcacac
     6841 gttggaaata ggttggaact ggatattttt ttaattgcat tgcgttttcc ttttatttaa
     6901 aaacgagaat ttgcgtttaa ttgggggcga agtatagcaa atacaatgca aaatagataa
     6961 aaaatcttta aggtgttgaa gtggctacaa agaaaaaaac agataggggc tttgtaggaa
     7021 ttccacttca acaatttggt tgtcttattc taaaataata ttggctcgat ttttttctac
     7081 cgttgcttgc ccaattccgg agacttcagc aagttgctca atgtttttaa aattgccgtt
     7141 tttagtacgg taatcgacaa tcgcttgagc ttttttctgc ccaattccca ctagcttgtc
     7201 ttgcaattca gcagcactag cagtattgat atttaccgca ttggcgttgg tgatttgtgt
     7261 ttgctgtacg ggttgagtga ctgcattttg ttcaacaggc attgtagttg gcggtgtttg
     7321 ggcaaaagcc attgcattta agccaaacaa gaatgttaga ccaagttgtt tgattttctt
     7381 cataggattt ccttattagt tgttgaaagg tcgaaaggaa gattaagagg gaataaaata
     7441 gaactcaacc ggcaaaaata aatttttcga cacagatcga aaaaatagaa aatggctaaa
     7501 cttctagact tgcatttggt ctgaaattca atgaaagtaa atagatttgc aaaaaattag
     7561 gcaaaaatga ccgctagttg cgtaaattta gttaaataaa ttcaaatagc ggctgaattg
     7621 ctttagaata tcgccatttt tatactcatt aacaacgcta ttatttggaa ttaatatgac
     7681 acaaaataat tttcgcaaag aaccggtttt tggtgaatct tcggttacct ctcaagacgc
     7741 tgaagcaacc aaagcaccgg ggcatacgtt taccccaatt atgaaaagac ctgatgctaa
     7801 agtagaagat catacggctg atgtcttttc ggctaagcag gcaaaagaat tccaatttac
     7861 tccaagttca gaagctacag aagcagtaga gccggataat accattcatt cggttgaaga
     7921 aagtgtatca accgcatctt ttaacacggt tgaagcacaa gaaaatgtgg aaaaagaagt
     7981 ggttaaacct gcacccgaga gtttcactac tgagcgtatt attccaaatg cggcaacagc
     8041 tgccgcagca gccactgcgg cagctgcagc cactaaaacc aaagagatta aagctaaaat
     8101 gccacctaaa acccgccgtt tattattagt ggcattgttg gcgttagcat tattggtact
     8161 gtttttcttg ttaaaaccaa gtacgccgga aactgtcgaa gagctacaat ctcagcaagg
     8221 tggtagcttg ccgattgaat tccgaccggt tgatgaagct gaagcgaaac atgcggaaga
     8281 acaagctcgt gcagaacaag aagccgctga agctgcaaaa gctcaagaac aagctcaagc
     8341 cgctcaacaa gagtctgtaa cagttgctcc agcccaaacc gagcaaacaa tggctaaacc
     8401 gtcacaagat agccagtcgc aaacacaatc acaagcagta gttgagcaac ctgttgcggt
     8461 gaaaccggtg gaaaataaac cggttgttgc acaaccggcg attaagccta aaacggaaaa
     8521 tagcgtggtg ttccaacctg aaacaacggt aaaacaacag cctaaagccc ctgctaaaac
     8581 tgaagtgaaa accgaacgtg ctacactggc agcaaaagaa acggcaaaaa cagcagctaa
     8641 accggttgag aaagctccga caaaaactga agcggtaaaa gcagcaccgg ctgccgagtc
     8701 ggcaaatgcg gtagcaacca aaacaatgac tgtgccaaaa gatgtatcac taatgcaagt
     8761 gttccgtgat aacaatttaa atatctcaga cgttaatgcg atgagcaaag tcaataacgt
     8821 ggtgagtaat ttaaaagtag gcgagcgagt aaccgttcgt ttagataaaa ataaccgtgt
     8881 ggttgaaatg agcattggct caggcggcaa atttacccgt caagcaaacg gtagctatac
     8941 ttttaaataa aatgaagaaa aagagcaacg ctacaaacgt tgctctttta tttaaaaagg
     9001 agataaagga tgaatttatt ttgcccctct tacaaactta tctacttaac gctaagctca
     9061 gcgatgttag ttgcttgtag tacagtggtt acccctgttg agcaaattca tcaagtacaa
     9121 aaggaaaaag agccagtaaa gaaagtgact aaactgatag cgcaagcacc taaagtgagt
     9181 ttatatcgtt gtgagaatga taagaaggtt gaagttgaac gccagggacg aacaaaaaaa
     9241 gcgttaaaaa aggaaatagt cacagtgagt tatcaaggga caacgcatca gctttcacct
     9301 tctgtaacga gagatggtaa aaaatacact aatatccgtt ggacttggca tgaaacccgt
     9361 agtggtaaag catttttgta taacaacact aaaaaaacgt tagcggcaaa ttgcataaag
     9421 cagtaacaag cggacacaaa aagtatcgct ttttgaacgt tggctttgcc aacggcagcg
     9481 aagctgtgaa caatccttac aaggattgtg aataaatttt taagagaatt ttgcaaatga
     9541 atattttagt tataggaccg tcttgggtgg gtgatatgat gatgtcgcac gcactttatc
     9601 aacagcttaa aatacagtat ccgaattgcc aaattgatgt aatggcaccc gattggtgtc
     9661 gtccgctttt agcccgtatg cctgaggtgc gtaatgcgat ttcaatgccg attggacacg
     9721 gttcatttcg tttgtgtgaa cgctaccgac tcggaaaatc cctgagaaat caatatgata
     9781 tggcgattgt attgcctaat tcattaaagt ctgcctttat tccactgttt gcgaaaattg
     9841 cgagacgtcg tggttggaaa ggggaaagta gatatttttt cttaaatgat cttcgcagta
     9901 ataaaaacga ctacccaatg atggtgcaac gctatgtagc cttggcgttt gaaaaggcta
     9961 ctgttccaac cgcacaacag ttgccgattg cttatccata cctcacaact gatagcgaac
    10021 aaattgagca gaccaaagcg aaatttgaaa aacagttggc ttatgcggaa aaccgtcctg
    10081 ccatcggctt ttgtccgggg gctgaatttg gaccggcaaa acgttggccg cattatcatt
    10141 atgcaacatt ggcaaaaatg ttgattgagc aaggctattc ggtacggtta tttggctcga
    10201 aaaaagatga agccgccggt gagcaaattc gcttggcatt acctgaaaat ttacaacgct
    10261 attgcctgaa tttagccggg caaaccgatt taaaccaagc ggtagatttg attgcagatt
    10321 gtaatgcagt cgtgagcaat gactccggct taatgcacat tgccgcagca ctaaataaac
    10381 cgctggtcgc cttatatgga ccgaccagcc cacaatatac gccgccgtta tcccaaaatg
    10441 ccgtgatcat tcgcttaatt gaaggcggtt tgattaaaat ccgcaaagaa ggggcagagg
    10501 gctatcacca aagtttaatt gatattcagc ctgaaatggt gatggacaaa ttagctgaac
    10561 tgttaattta aatggataac aagcggttga attttgccaa ttttttgcaa aaaaagaccg
    10621 cttgtttgtt tataaaagat gaaaatttta atcgttaaaa cctcatcaat gggagatgta
    10681 ttacatacgc ttccagccat aaccgatatg caaaatgcta ttcccgattt aaaagtagat
    10741 tgggtagtgg aggagaattt taccgaaatt cctgcgtggc attcagcggt aaataaagta
    10801 attccggtgg ctattcgccg ctggcggaaa aacctttgcc aaagacagac ttgggtagaa
    10861 tggcagcagt ttttaaaaca gctaaaaagt gagcagtatg atgcggtaat tgatgctcaa
    10921 gggctgatta aaagtgcggt gttagtaact cgccaagcca aaggcaaaaa atttggctat
    10981 gataaaaact ctgcccgaga agggttaagt agtctgtttt acgatgaaac gtttaatatc
    11041 ccctaccaac aacacgctgt agaacgcatc agaaaattat gtgcaaaatc attaggttat
    11101 gctttgccaa ccgaacgggg cgattattgc attgcacaac attttgcaaa aaatcctcaa
    11161 aaaacgaccg cttacattat gctgattcac gccactaccc gagcggataa acattggcaa
    11221 gaagcctatt gggcggagtt gattcaagcg ttggataagc acaatctggc agtcagattg
    11281 ccttggggaa accaagccga aaaagaacgg gcggagcgtt tagcgagtgt ggcgaataat
    11341 gctaaggtat tgccaaaaat gagcttaacc gatattgcta ctcaacttgc gggagcagcg
    11401 gcagtggtgt cggtggatac cggcttgagc catttggcgg ctgcgttaga taagccaaat
    11461 gtgattttat atggggcaac cgatccaaaa cttatcgggg cttacgggca aaatcagttt
    11521 tatctaaaag cagataaaat ggaaaatatt acctcttcac aagtgctgga aaaattactt
    11581 ccattgatta acaaatgatc gttgttgtta tcttactgtg cttgactttt atccacattc
    11641 aatttaaaat ttgccgtcct ttcgtgtgaa aggatgtttt taattttatt tttaataatt
    11701 ttaactggag cttttttaat ggcaactatc aaccagctag tacgcaaacc gcgtgtgaaa
    11761 aaggttgtaa aaagtaacgt tcctgcatta gaggcttgcc cgcagaaacg tggcgtgtgt
    11821 actcgtgtat acactactac acctaaaaaa ccgaactcag cattacgtaa agtatgtcgt
    11881 attcgtttaa caaatggctt tgaagtaact tcatacatcg gtggtgaagg tcacaacctt
    11941 caagaacaca gtgttgtatt aatccgtggt ggtcgtgtta aagacttacc gggtgttcgt
    12001 taccatactg tacgtggtgc acttgactgt gcaggcgtta aagatcgtaa acaaggtcgt
    12061 tctaaatacg gcgttaaacg tcctaaagct taatggttct ccgttgagta aggccaaacg
    12121 tctaaatttt aatatttaaa ccataaactc cagtcaagat gccttaaggc agattagatg
    12181 agttttggat atcctgaaga tttaatatac ggagaaattt ccaatgccac gtcgtcgtag
    12241 tattgaacca cgcaaaattc ttccagatcc gaagttcggt tcagaattac ttgcaaaatt
    12301 tattaacgtg ttaatggtag atggtaaaaa atctactgca gaaaaaatcg tttacggtgc
    12361 attagaaact ttagcacaac gttctggtaa agaagcgtta gaagctttcg aaatcgcatt
    12421 agaaaacgta cgtccaactg ttgaggttaa atctcgccgt gtgggtggtt ctacttacca
    12481 agttcctgta gaagttcgcc caacccgtcg taatgcttta gcaatgcgtt ggatcgtaga
    12541 agccgctcgt aaacgtggtg acaaatcaat ggcattacgt ttagctaacg aattatcgga
    12601 tgcagcagac aacaaaggtt cagcagtgaa gaaacgtgaa gacgttcacc gtatggctga
    12661 agctaacaaa gcgtttgctc acttccgttg gtaatcgttt aagtaagctt atagaaggct
    12721 tcatctcagt ttgcggtgaa gccttaccct tattaagaat tttaatttaa accccaaaca
    12781 aggtaataaa taaaaatggc tcgtacaacc cctatttcac tttaccgtaa catcggtatc
    12841 agtgcgcaca tcgatgcggg taaaacaact actacagagc gtatcttgtt ctatacaggt
    12901 gtaagtcaca aaatcggtga agttcacgat ggtgctgcaa caatggactg gatggaacaa
    12961 gaacaagagc gtggtatcac gatcacatct gctgcaacaa cagcattctg gtctggtatg
    13021 tcacaacaat atccacaaca ccgtatcaac gttattgata ctccgggaca cgttgacttt
    13081 actatcgaag tagaacgttc aatgcgtgta cttgatggtg cggtgatggt ttattgtgca
    13141 gttggtggtg ttcaaccaca atctgaaact gtatggcgtc aagctaacaa atataaagtg
    13201 ccacgtatcg cgttcgtaaa caaaatggac cgtacaggtg caaacttctt acgtgttgtt
    13261 gagcaaatca aaacgcgttt aggtggtaac tcagttgcat tacaacttcc aatcggtgca
    13321 gaagacagct tcaaaggtgt tgttgattta atcaaaatga aagcaatcaa ctggaacgaa
    13381 gctgatcaag gtatgacctt cacttatgaa gatattccgg cagatatgct tgaagcttgt
    13441 gaagaacacc gtgcaatgct tgttgaagca gcagctgaag cttctgaaga attaatggaa
    13501 aaattcttct ctggtgaaga attaactgaa gatgaaatca aagcagcatt acgtcaacgt
    13561 gtattagcag gtgaagtaat ccctgtttgc tgtggttctg catttaaaaa caaaggtgta
    13621 caagcaatgc ttgatgcggt aatcgattac ttaccggcac caactgatat tcctgcaatt
    13681 aaaggtatca atgaagatga aactgaaggt gagcgtcacg caagcgacga tgagccattt
    13741 gcttcattag cattcaaaat tgcaacagac ccattcgtag gtaacttaac cttcttccgt
    13801 gtttactctg gtgtaattaa ctctggtgat acagtttaca actcagtaaa acaaaaacgt
    13861 gaacgttttg gtcgtatcgt acagatgcac gcaaacaaac gtgaagagat caaagaagtt
    13921 cgtgcgggcg atatcgctgc agcaatcggt ttaaaagatg ttggtacagg tgacacatta
    13981 tgtgcaatcg atgcaccaat cattcttgag cgtatggaat tccctgagcc ggtaatctct
    14041 gttgcggttg aaccaaaaac caaagctgac caagagaaaa tgggcttagc tttaggtcgt
    14101 ttagcacaag aagacccttc attccgtgtt cacacagatg aagaatctgg tgaaacgatc
    14161 atttcaggta tgggtgagtt acacttagac atcatcgttg accgtatgaa acgtgagttt
    14221 aaagtggaag caaatatcgg taaaccacaa gtatcttacc gtgaaactat ccgtactcgt
    14281 gttaatgatg ttgaaggtaa acacgcaaaa caatctggtg gtcgtggtca atatggtcac
    14341 gttgtgatcg acttataccc attagatgct gaaggtccgg gttatgagtt tgtaaacgag
    14401 atcaaaggtg gtgtaatccc tggtgaatac attcctgcgg ttgataaagg tatccaagaa
    14461 cagcttaaat ctggtccgtt agcaggttac ccggtagtag atttaggtgt gcgtttacac
    14521 ttcggttcat accacgatgt tgactcatct gaacttgcgt ttaaacttgc tgcatcatta
    14581 gcatttaaag cggcgttcgc aaaagcaagc ccggtattac ttgagccaat catgaaagtg
    14641 gaagttgaaa ctccaccgga ttacgtgggt gatgtaatcg gtgacttaag ccgtcgtcgt
    14701 gcaatggtta acggtcaaga agcgaacgag ttcgttgtta agatcaatgc tgaagttcca
    14761 ctttcagaaa tgtttggtta tgcaacagac ttacgttcac aaactcaagg tcgtgcatca
    14821 tactctatgg aacctttaaa atatgctgaa gcaccgaaaa acgttgctga tgcaattatt
    14881 gaagctcgta gaaaataatt tgtttaacct gagccatagc aatagggcta tggctcattt
    14941 tttaggaaga tattaaa
//
LOCUS       DANKBV010000053        14545 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.053, whole genome shotgun sequence.
ACCESSION   DANKBV010000053 DANKBV010000000
VERSION     DANKBV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14545
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.053"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(6..416)
                     /locus_tag="PWY43_001302"
     CDS             complement(6..416)
                     /locus_tag="PWY43_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136182.1"
                     /translation="MFIPKSSKLKTNNYIEELNIKSIGETDMKKALTLLALSLFALPV
                     QADSLELARNKVKQIFISDEEPKVKDATWTAPFIFKVGVFDDGTKRDGYAEYVCQVLY
                     DHGFKGKGVLVSVIDIKKLVQTGDWVDLGKAKCQ"
     gene            complement(434..952)
                     /locus_tag="PWY43_001303"
     CDS             complement(434..952)
                     /locus_tag="PWY43_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136183.1"
                     /translation="MEHQNLTHLVYITSHKQIGVYSISNLNIDDLYFKGYCIQKNRVI
                     TLRADRIIKQFDDLALAENYAKNIPQDVFYLFDSLLNQQRKEKITPIYKIGLCFTGFK
                     QARKNELIQLAVDNDLKVVQNVTGAVDFLIFDKESKTVGPAKLAKAEKLGIKIINDEE
                     FLYMLETGVVPD"
     gene            complement(956..1642)
                     /locus_tag="PWY43_001304"
     CDS             complement(956..1642)
                     /locus_tag="PWY43_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2136184.1"
                     /translation="MDTLASRLKIVLDQKGLTQEKFAEQIGVSQPSVFKILNGQTRNP
                     TRIYEIANVLDVDVNWLKTGKGEAPDFAKIGKNPTAYEEESSMLRLEVLDVYASAGNG
                     SFVTGDLTAYTHAVEFENAYFAQVFQRANAKGLSIINVDGDSMEPTIGNGDLLFVDTT
                     KSAYQGDGVYVFSYGENLYVKRLQFAGDELLVISDNPLYKEWRITSENEHKFQIHGKV
                     EFMQGHIRRV"
     gene            1766..1978
                     /locus_tag="PWY43_001305"
     CDS             1766..1978
                     /locus_tag="PWY43_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2136185.1"
                     /translation="MTNKKIQKAIELVGTQTKLAEACGVSQPTVLKWLNGGGISAEYL
                     LKIEAATNGQVTIREICEELGNATAK"
     gene            1962..2246
                     /locus_tag="PWY43_001306"
     CDS             1962..2246
                     /locus_tag="PWY43_001306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136186.1"
                     /translation="MQQQNKSPEQAGLASGKFTVRRNQDGKWRLDLTLLDFGISSPET
                     TQARCKLLQELQLALLQGQGHLNTVGVNVRKAGPVVCVGSLLSDLLNHQE"
     gene            2462..2734
                     /locus_tag="PWY43_001307"
     CDS             2462..2734
                     /locus_tag="PWY43_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136187.1"
                     /translation="MNLDHTCTNCKSKNLRVRTSEKIGLLTIQAVTYCNNCGTEQLVQ
                     SQIIRVRTPIYKERPEAMRINKCLMEADLNTPDLFDGVVADQPKQD"
     gene            2817..3149
                     /locus_tag="PWY43_001308"
     CDS             2817..3149
                     /locus_tag="PWY43_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136188.1"
                     /translation="MSKQLYYRHKSRWNRLNRLKKMNKRRLNLFLQEKRVANLESRLA
                     DEVELAKLNSEQIGDLLQALELKVNRLIRQNEKLKARVIKLEKVKQTQKQGLFAMFKK
                     VLGSKGNP"
     gene            3158..3451
                     /locus_tag="PWY43_001309"
     CDS             3158..3451
                     /locus_tag="PWY43_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136189.1"
                     /translation="MSLVAMFQKQKVSNSKKAFSCDHFGLMWNQASEEHKRLLLRAAF
                     LQENPEPKPWGAYSRQEKSTLHSFGLWFEEFADFQRVMKNDSKLREKRINAPD"
     gene            3602..3844
                     /locus_tag="PWY43_001310"
     CDS             3602..3844
                     /locus_tag="PWY43_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136190.1"
                     /translation="MSLITVNDYKIKKDILDADGKPCYQLYQGEELLRDTTNLSEIIA
                     YMFIRAEDKPFDELYNHIINQEAGAKTDIKFTVTVR"
     gene            3841..4173
                     /locus_tag="PWY43_001311"
     CDS             3841..4173
                     /locus_tag="PWY43_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136191.1"
                     /translation="MSQITKSAYTHIHPNWQAPSGNTYHIERYELKRRYALFCNGERV
                     VLYKDPTHPNKNVESHIESDVLLLWLLKNELGWSGNIKEPLSPQDVENIRLLEQAFCL
                     TYNGTFIK"
     gene            4170..6530
                     /locus_tag="PWY43_001312"
     CDS             4170..6530
                     /locus_tag="PWY43_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDL2136192.1"
                     /translation="MNYQNEILSATEAFAIGVSLPPVAGASACALELHSDRLYQAFKQ
                     PYTSMVQQELFSKDPIHEPLRQSYFERLPRALAEHFGRQYKQKLHYQSQYDTADWFKA
                     EMARKMPRIEAVISQYCDVFDFLKQSHQDLDFLAELDSNVYFGAGVDTQQAVQKIKQD
                     AEVKGIQLPMHLCRLENSSRYLKAHGIRPLAYQTEEQIKQLALGIAYRVRQIQQDNIE
                     QNMHKATDGDTAYSVLLECYRAMMGEVNKLKIDSPYQKKARKGRLTEEEITTGFLKMT
                     CEKWWTRKLSKIAEQMKEHLAIACGMVNMLSPYCSNARLKAFEAQRKANIDYLKSMII
                     ANIAEPEEQLSLFETWLKSASNPKIKRLELLTRMNGFERYADKQGHEGWFITLTAPSK
                     YHAMLSRTSSVNPKWNGASPAETQVYLVNTWAKIRAKLNREGVMAYGFRVAEPHADAT
                     PHWHLILFTRPEDMEKLRRVFLSYALEVDGTEAGAKKYRCKFKRIEKEKGSATGYLVK
                     YVSKNIDGFGMDGELSDEANIQAKENAARVGAWSSVWCIRQFQQLGNIPISLWRELRR
                     LGSVEQEDETLEKLRVIADSGEWDVFTEELGGALVKRADLVARITYTERKSETGEALY
                     TMYQEPSLKVSGIINIKNGVQINTRPKEWSIQLKPKNWEEQQLQKKLENATETERAEA
                     KRKYYEKLGFNEEAIALLGELAPPWTCVSNCTGSKNNQISEEARNCLRNELITMRGRV
                     TEHQIDDLLNGKRLKIWGNSQKTMFVSYSRGRLIEHIVDNYESFLN"
     gene            6543..6875
                     /locus_tag="PWY43_001313"
     CDS             6543..6875
                     /locus_tag="PWY43_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136193.1"
                     /translation="MVKFIRLNLINYFIEKDEYGEPVRCRAFRYDEIIQTKFIEGFRE
                     GEIPADDYGFEDWNGSVLILNEEGRKYFENYLFDVKKNYCRARDNYVYVLETLDEIEM
                     KLGLLEAK"
     gene            6875..7327
                     /gene="ssb"
                     /locus_tag="PWY43_001314"
     CDS             6875..7327
                     /gene="ssb"
                     /locus_tag="PWY43_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2136194.1"
                     /translation="MNLVTLVGRLGQDPDIRTMQNGEKAAALSVATSEKWTDKQTGVK
                     KESTEWHRVVLYRRLAEIAELYVKKGHLVSIIGKIKTRKWTDSNGVERSITEIIAEQM
                     QMLSSGEKNTPNTPNKAENKPQPKQKNQDVMTADEQKDIPQFDDDIPF"
     gene            7338..7670
                     /locus_tag="PWY43_001315"
     CDS             7338..7670
                     /locus_tag="PWY43_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136195.1"
                     /translation="MREWEKQMPFSANLHIRPTGKFEVKNVNIEQIIGERGKNYGNFD
                     DVATMSQQLKAVLLDTNTQLKPHQKEAGEMICLKLARIFAGCNPDYEDNWRDIAGYAV
                     LGGKLNEK"
     gene            7660..7929
                     /locus_tag="PWY43_001316"
     CDS             7660..7929
                     /locus_tag="PWY43_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136196.1"
                     /translation="MKNKMGSKFIRVRVEHPQRKKEDIELVQDVVAYHIFNLFKFEES
                     GLRGFSQYDLEIDVKVKPVLEQKEMPVDVWIDDLGGKNGTITSGQ"
     gene            7904..8194
                     /locus_tag="PWY43_001317"
     CDS             7904..8194
                     /locus_tag="PWY43_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136197.1"
                     /translation="MEQLQAVSNEQVFAKLCEVERLLKTKGVNEHSRELWDLGDVAAY
                     FGYTKEHTSRSVVSSPHFPKPIALDGLRGKGRGAKKWVSGEVVKFCLMWKVK"
     gene            complement(8464..8646)
                     /locus_tag="PWY43_001318"
     CDS             complement(8464..8646)
                     /locus_tag="PWY43_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDL2136198.1"
                     /translation="MAMSRNEIQAKSEAKRGIKQKSFKLPLEVIAEIEVLSQKLNIPQ
                     NQLIIQAIQQFKQNNP"
     gene            complement(8924..9922)
                     /locus_tag="PWY43_001319"
     CDS             complement(8924..9922)
                     /locus_tag="PWY43_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2136199.1"
                     /translation="MATIIKNGNKYRAQVRKKGIYKTATFSTKTEANRWAYALEAQID
                     AGEYNTTPNILFAELIDKYLKEVTPTKRGKREERYRLLRIAGSNLGNIPLPALTKEHL
                     RQWQNQRLQEVQPVSVSRERATLSALFTKAMEWDYLKENPLKTLEKIKTPPARTRRYS
                     DEEIDKLIFVSGYSPNQPPLLMQQRAGAALLFAIETAMRAGEIAGLKWEHINLQARTA
                     FLPQTKNGYSRTVPLSTKAIAILNQLAQLEPNKQNSVFQLTSRSLDSNFRKLKQKANL
                     HNADLHFHDTRREALTRLSKYLTVMELAKVSGHRDLSILQNTYYNPDISELVSKLQ"
     gene            complement(10150..10226)
                     /locus_tag="PWY43_001320"
     tRNA            complement(10150..10226)
                     /locus_tag="PWY43_001320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10190..10192),aa:Val,seq:gac)
     gene            complement(10290..11102)
                     /gene="apaH"
                     /locus_tag="PWY43_001321"
     CDS             complement(10290..11102)
                     /gene="apaH"
                     /locus_tag="PWY43_001321"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDL2136200.1"
                     /translation="MATYIVGDLHGCFNEFQLLLEKVNYNPKQDELFLTGDLVARGED
                     SLACLRFVKDSANNAKTVLGNHDLHLLATMLGIKRVKPNDKIDAIFEAKDRLDLQNWL
                     RNQPLVIHHPKHQFLLTHAGISPEWDLATTLACAKEAEKVLQSDNYADYIAQMYENKP
                     ERWSEDLQSVERWRYIINVFARMRFCYTDKRLDFDCKLPLEQAPQELLAWFDLDNPLF
                     TQKNIIFGHWASLMGKCTRPNIYALDTGCAWGSHLTMLRWEDKQIFTQKRLK"
     gene            complement(11194..13170)
                     /locus_tag="PWY43_001322"
     CDS             complement(11194..13170)
                     /locus_tag="PWY43_001322"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDL2136201.1"
                     /translation="MFQNNPLLAQLKQQIEASKEYVAGVVKASDKAFGFLECDKKSYF
                     IPPAEMKKVMHGDSVKAVVKRDGDKEQVEIDSLLEPMLDRFIAQVRLNKEGKLQLAVD
                     HPSIKQTIPANTHKKVTEKLENGDWVVAQLKTHPLRDDRFFFAQVTQFICKESDNFAP
                     WWVTLARHEQPREPVANEKNYELHDELAREDLSHLYFTTIDSPSTQDMDDALFIEPIK
                     QNGKRTGWRLVVAIADPTAYIPENSHIEKAARQRCFTNYLPGFNIPMLPRELSDDLCS
                     LVPNEKRPALVGFIETDLNGNLIKETTFTSAWVESKDRLAYDDVSDYLEGVENAWQPQ
                     SEETKQQIEWLHQFTQARIQWRAENALLFKEQGDYTFELNEDGSVKDIHVEYRRIANQ
                     MIEESMILANICCAKFLSQHAKTGVFNTHSGFDPKNYELVKSFLLQTLASDENRDELA
                     ERYSAERLATLEGYCQMQRDIQDYPEKFLELRLRRYLTFAEFKAEVAPHFGLGITDYA
                     TWTSPIRKYGDMVNHRLIKQVLLGKQSQAVEESVLSRLQEARRQNRLVERDIADWLYA
                     RYLAPMVEQAVEFECEIADVSRGGLRAKVIKNGAQIFVPFSSLHDNKEEMEYRQEELA
                     LYIKGEKAYQIGQAVKVKLTEVRLETRSIVGNII"
     gene            13330..13608
                     /locus_tag="PWY43_001323"
     CDS             13330..13608
                     /locus_tag="PWY43_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL2136202.1"
                     /translation="MKKLLAIATVFSLAACSTQTLELSPAKEAQYDRVQHFFVSGFAQ
                     KQEIDAAKICNGADKVGKVETETTFFNGLLGTITYGIYSPRQIRVYCN"
     gene            13633..13929
                     /locus_tag="PWY43_001324"
     CDS             13633..13929
                     /locus_tag="PWY43_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL2136203.1"
                     /translation="MKKLLAMSVLTALLTTACTTQTAHIGGKVTDNIKPTKSVTQSFF
                     IGGIGQEETLNVVEVCGSKDKVQQVETVLSGGDILLGLVTIGIYTPRTANVYCK"
     gene            complement(13982..>14545)
                     /locus_tag="PWY43_001325"
     CDS             complement(13982..>14545)
                     /locus_tag="PWY43_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2136204.1"
                     /translation="ELSKHWEKFDGFIHAIAFAPGDQLDGDYVNAATREGYRIAHDIS
                     AYSFVAMAQAARPFLNENATLLTLSYLGAERAIPNYNVMCLAKASLEAATRVMAADLG
                     KEGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRRTVTIDDVGNSAAFLCSDL
                     ASGVTGEVLHVDAGFSVMAMGELGDEE"
BASE COUNT         4349 a         2617 c         3217 g         4362 t
ORIGIN      
        1 atgtattact gacatttagc tttgccaaga tcaacccaat cacctgtttg gactagtttt
       61 tttatatcga ttacgctaac caacacacct ttacctttaa atccgtggtc gtataaaact
      121 tggcaaacat attcggcgta gccatctctt ttcgtgccgt catcaaaaac tccaacctta
      181 aagataaatg gtgcagtcca agtcgcatct tttacctttg gctcttcatc actgataaaa
      241 atttgcttta ctttattacg agcaagttcg aggctatcgg cttgtacagg caatgcaaat
      301 agtgatagtg caagtagtgt aagtgctttt ttcatatcgg tttcccctat tgatttgatg
      361 tttaattctt ctatgtaatt gttagttttt agtttagatg attttggtat aaacatctat
      421 tgagaagtca aacctaatcc ggcacaacgc cagtttctaa catatataaa aattcttcat
      481 cgttgatgat tttaatacct aacttttcag cttttgctag ttttgctggt ccaacagttt
      541 tcgactcttt atcgaaaatt aaaaaatcaa ctgcaccagt tacgttttgt accactttca
      601 aatcattatc tactgctagt tgaattaatt cattttttct agcttgctta aatccagtaa
      661 aacacaaacc gattttatag atcggtgtta ttttttcttt tcgttgctgg ttcagaagag
      721 aatcaaatag ataaaacaca tcttgaggaa tatttttggc ataattttcg gcaagagcta
      781 aatcatcaaa ttgtttaata atgcgatcag ctcttaatgt gataactcta tttttctgaa
      841 tacagtaacc tttgaagtaa aggtcatcaa tattcaaatt agaaatcgaa taaactccaa
      901 tttgtttatg tgatgtaata tagacaagat gagttagatt ttgatgttcc atcacctaaa
      961 ccctcctaat atgcccttgc ataaattcca ctttaccgtg aatttggaat ttatgctcat
     1021 tttctgatgt gattctccac tctttataaa gcggattgtc ggaaatgact aacaattcat
     1081 caccggcgaa ctgcaagcgt tttacatata agttttcgcc atagctgaaa acatagacac
     1141 catcgccttg ataagcggat ttagtggtat ctacgaacag caaatcaccg ttaccgatag
     1201 ttggctccat tgagtcgcca tctacattaa taattgataa gcctttggca tttgcacgtt
     1261 gaaagacttg tgcaaaatag gcattttcaa attctactgc gtgcgtgtaa gcggttaagt
     1321 ctcccgtgac aaaactacca ttgccggcag aggcatatac atctaacacc tcaagtcgaa
     1381 gcattgagct ttcctcttca taagcggtcg gattttttcc gattttcgca aaatctggtg
     1441 cttcgccttt gccggttttg agccaattta catcgacatc taggacgttt gcaatttcat
     1501 aaatccttgt gggatttcga gtttgtccat tcaggatttt aaaaacagat ggctgtgaaa
     1561 cacctatttg ttctgcaaat ttttcctgtg ttaaaccttt ttggtctaaa acgattttca
     1621 aacgacttgc caaagtatcc attaggaact ccttattttt gatgagtata taactaaagg
     1681 tatagaaata aaaattcatt tggttattga ttttttattt ctttggttat attattataa
     1741 ccattcaata accagagtta tagttatgac taataagaaa attcagaaag ctattgaact
     1801 tgtaggaaca caaacaaaat tagctgaagc ttgtggagtt agtcagccta cggtcttgaa
     1861 atggctaaac ggaggcggaa tttccgctga atatctacta aaaatcgaag ctgccacaaa
     1921 cgggcaggta acaattcgtg aaatttgtga ggagttggga aatgcaacag caaaataaaa
     1981 gccctgaaca ggcagggcta gcgagtggga aatttaccgt aagacgtaat caagacggta
     2041 aatggcgttt agatttgacc ttgttagatt ttggtattag ttcgccagaa acgactcaag
     2101 ctcgttgcaa attgcttcaa gagcttcagc ttgctcttct tcaaggtcaa ggtcatttaa
     2161 atactgtcgg cgtgaacgtt cgaaaagccg gtccagtcgt ttgcgttggt agtctgttaa
     2221 gcgacctttt aaatcatcaa gaataatgtt tttggcgttg agttgatggc gtaagttttc
     2281 aacttcaaca gtcagttcaa taatttgatc ttcagtattc ataaaatttc cttttggagg
     2341 gttaaaaata tgtaaaacat aaccaaaatt caaccgcttg tctatgggga tagataagcc
     2401 gaaacaacag taaaaacgca aagaatgtga atagagcctt ggggaaggcg taggggaaat
     2461 aatgaattta gaccacacct gtacaaattg caaaagtaaa aatttacggg ttcgtacatc
     2521 tgaaaaaatc ggtttgctga ctattcaggc tgttacttat tgtaataact gcggcacaga
     2581 gcaacttgtg caaagtcaaa ttatccgtgt tcgcacaccg atttataaag agcgaccgga
     2641 agcaatgcgt attaataaat gcttgatgga agccgatttg aacacgccgg atttgttcga
     2701 tggcgttgtg gcagaccaac ctaagcaaga ctaatcccaa tttttaaaca atcgtcaaaa
     2761 taagccttat ttgcataggg cgagattttt gcaccctaaa ttcaggaatt tgaacaatga
     2821 gtaagcaatt atattaccga cacaaatcac gttggaacag acttaaccgc cttaagaaga
     2881 tgaataaacg tcgccttaat ctgtttttgc aagaaaagcg ggtggcgaat ttagaaagcc
     2941 ggttagctga tgaagtagag ctggcaaagc tgaatagtga gcagattggc gatttactgc
     3001 aagcgttgga gttaaaagtg aatcggttaa ttcgccaaaa tgaaaagctg aaagcccgtg
     3061 tgattaagct ggaaaaagtc aaacagaccc aaaagcaagg tttgtttgca atgtttaaaa
     3121 aggtgttggg tagcaaaggg aatccttagg ggttggtatg agtttggtgg caatgtttca
     3181 aaagcagaaa gtgagtaata gcaaaaaggc gtttagttgc gatcacttcg gcttgatgtg
     3241 gaatcaggca agtgaggagc ataagcggtt gttattgcgt gctgcctttt tgcaggaaaa
     3301 cccagagccg aaaccgtggg gagcttatag cagacaagaa aaaagcacct tgcattcatt
     3361 cgggttgtgg tttgaagaat ttgccgattt ccaacgagta atgaagaacg attccaaatt
     3421 gcgtgaaaaa cgcattaatg cacctgatta ggagggaaat atgagccgta accgtgaatt
     3481 gtgtgagttt tttcacaaac tgtggtgcaa aaccggtaat cgctatgctt atcgccaata
     3541 tcaagcgttt tttaaacggt atcagaccga atgtttagtt tattagttag agggtagaag
     3601 aatgtcatta attactgtga acgattacaa aatcaagaaa gatattttag atgcagacgg
     3661 taagccgtgt tatcagttgt accaaggcga ggagctattg cgtgatacca ccaatttaag
     3721 cgaaatcatc gcttatatgt ttattcgtgc cgaagataaa ccgtttgatg agttgtataa
     3781 ccatatcatc aaccaagaag caggagctaa aacagatatt aaatttacgg taacggtgag
     3841 atgagccaga ttactaaatc cgcttacaca catattcacc caaactggca agccccgagc
     3901 ggtaacacct accacattga gcgatatgag ctaaaacggc ggtatgcctt attttgtaat
     3961 ggtgagaggg tagttttata taaagaccca acgcacccca ataagaacgt agagagccat
     4021 atagaaagtg atgttttgtt gttatggcta ctgaaaaatg aattagggtg gagcggtaat
     4081 attaaagagc cgttaagccc tcaagatgtt gagaatatcc gcttgttaga gcaagcattt
     4141 tgtttaactt ataacggaac tttcattaaa tgaattatca gaatgagatt ctatctgcta
     4201 cagaggcttt cgcaataggt gtttcactcc cacctgttgc cggtgcttct gcttgtgcat
     4261 tagagcttca ttctgatcgt ttatatcaag cgttcaaaca gccttacact tcaatggttc
     4321 agcaagaatt attctcgaaa gaccctattc acgaaccgtt acgccaatct tattttgagc
     4381 gtttgcctcg tgccttagca gagcatttcg gcagacagta taagcaaaag ctacattacc
     4441 aaagccaata cgacacagca gactggttca aagcggaaat ggcacgcaaa atgccacgca
     4501 ttgaggcagt tattagccag tattgcgatg tattcgattt tctgaagcaa tcccatcaag
     4561 atttagattt tttagctgaa ttagatagta atgtttattt cggtgccggt gttgatacgc
     4621 aacaagcggt acaaaaaatt aaacaagatg ccgaagtgaa aggtattcaa ctgcctatgc
     4681 acctttgccg tttagagaat agctctcgct atttaaaagc tcacggcatt cgcccgcttg
     4741 cttaccaaac cgaagaacaa atcaaacagc ttgccttagg tattgcttat cgtgttcgcc
     4801 aaattcagca agataacatc gagcagaata tgcacaaagc gacagatggc gacacagcct
     4861 atagtgtgtt gcttgaatgc tatcgggcaa tgatgggaga ggtaaataag ctcaagattg
     4921 attcgcctta tcagaaaaaa gcgagaaagg gtagattaac tgaagaggaa attaccacag
     4981 gctttttaaa aatgacctgt gaaaaatggt ggactagaaa gttatctaaa attgccgaac
     5041 agatgaaaga gcatttagct attgcgtgcg gtatggtgaa tatgctttcg ccttactgtt
     5101 caaatgctcg cttgaaagcg tttgaggctc aacgcaaggc gaatattgat tatttgaaat
     5161 caatgatcat tgccaatatc gccgagccgg aagagcaact ttcattattt gaaacgtggt
     5221 tgaagtctgc ctctaacccg aaaattaaac gccttgaatt actcactcgt atgaatggat
     5281 ttgagcgtta tgcagataag caagggcacg aggggtggtt tattaccttg actgctccgt
     5341 ctaagtatca cgcaatgctt tcaagaacca gtagcgtgaa cccgaaatgg aatggagcaa
     5401 gccctgcgga aacacaggtt taccttgtga atacttgggc gaaaatccgt gccaaactca
     5461 accgtgaggg cgtaatggct tacggttttc gagtggctga acctcacgct gacgctaccc
     5521 cacactggca tttaattcta ttcactcgtc cggaagatat ggaaaagctt cgccgcgtgt
     5581 tcttgagtta tgccttagag gtcgatggca cagaagccgg tgcgaaaaaa taccgttgca
     5641 aattcaagcg tattgagaaa gagaaaggct cggcaacggg ctatttagtg aaatatgttt
     5701 ctaaaaacat tgacggtttt ggaatggacg gcgagctttc agacgaggca aatattcagg
     5761 cgaaagagaa tgccgctcgt gtaggagcgt ggtctagcgt ttggtgtatt cgacagttcc
     5821 agcagttagg taatattccg atcagcttat ggcgtgagtt acgccgtttg gggagtgtag
     5881 agcaagaaga tgaaacttta gagaaactcc gtgtgattgc agatagcggt gagtgggacg
     5941 ttttcaccga agaattaggc ggtgcgttgg ttaaacgtgc cgatttagtt gcacgtatta
     6001 cttataccga acgcaaaagc gaaaccggtg aggcgttata tacgatgtat caagagccat
     6061 cattaaaagt gagcggcatt attaacatta aaaacggtgt gcagattaat acacgcccga
     6121 aagagtggag cattcaactt aaacctaaaa actgggagga gcaacaatta cagaaaaaat
     6181 tagaaaatgc aaccgaaacg gaacgagcgg aagctaaacg taaatactac gagaagttag
     6241 ggtttaacga agaggctatc gctttattag gggagcttgc tcccccttgg acttgtgtca
     6301 gtaactgtac ggggtcaaaa aataatcaga ttagtgaaga ggctcgaaat tgcctaagaa
     6361 atgaactgat tacaatgcga ggacgggtta ctgaacatca aatagacgat ttactaaacg
     6421 gcaagcggtt aaaaatttgg ggaaattcgc aaaaaacaat gtttgtgagt tatagccgtg
     6481 ggcgtttaat tgagcatatt gttgataatt atgagagttt tttgaattag gatattaaga
     6541 aaatggttaa atttattcgt ttaaatttga ttaattattt cattgagaaa gatgaatatg
     6601 gagaaccggt gcgttgcaga gcgtttcgtt atgatgagat tattcaaact aagtttattg
     6661 aaggatttcg agagggagag ataccagctg atgattatgg atttgaagat tggaatggtt
     6721 cggttctgat tttaaatgaa gaaggacgta aatattttga gaactatctt tttgatgtaa
     6781 agaagaatta ttgtagagca agggataact atgtatatgt cttggaaact ttagacgaaa
     6841 ttgaaatgaa gttaggatta ttggaggcga aataatgaac ttagtcacat tagtcggacg
     6901 tttggggcaa gaccctgata ttagaacaat gcaaaatggt gagaaagctg cggctttatc
     6961 ggtagcgacc tctgaaaagt ggacagataa gcaaacaggt gtgaagaaag aaagcaccga
     7021 atggcatagg gtggtgcttt atcgccgatt agctgaaatc gccgaattgt atgtaaaaaa
     7081 agggcatttg gtatcaatta tcgggaaaat taaaacccga aaatggacgg atagcaacgg
     7141 tgttgagcgg agtattacag agattattgc cgagcagatg caaatgctca gtagtggtga
     7201 gaaaaatacg ccaaatacgc caaataaggc agaaaataaa ccgcaaccaa agcagaaaaa
     7261 tcaagacgtg atgactgcgg acgagcagaa agatattccg cagtttgatg atgatattcc
     7321 gttttagggg gtgaataatg agagaatggg aaaaacaaat gccatttagt gcaaatttac
     7381 atataagacc tacaggaaaa tttgaagtga aaaatgtaaa catagaacaa ataatcggag
     7441 aacgtggcaa aaattacggc aactttgatg atgttgccac aatgagccag cagttaaaag
     7501 cagtgttgct ggataccaat actcaactta aaccacatca aaaagaggca ggcgagatga
     7561 tctgcttgaa actggctcgc atttttgcag gttgtaatcc ggactatgaa gataactggc
     7621 gagatattgc gggttatgcg gtgctgggag ggaaattaaa tgaaaaataa aatggggagt
     7681 aaatttatac gagtcagagt tgagcatcca cagcggaaaa aagaagatat tgagcttgtg
     7741 caggatgtgg ttgcttatca tatttttaat ctttttaaat ttgaggaaag tgggttacgg
     7801 gggtttagtc agtatgattt ggaaattgat gtgaaagtta agccagtgtt agaacaaaaa
     7861 gaaatgcctg tagatgtttg gattgatgat ttaggtggta aaaatggaac aattacaagc
     7921 ggtcagtaat gagcaagttt ttgcaaaact ttgtgaggtt gagcggttat taaaaaccaa
     7981 aggggtgaat gagcatagcc gtgaattgtg ggatttgggc gatgtagcag catatttcgg
     8041 ctacactaaa gaacacacca gccgaagtgt tgtatctagt ccacattttc cgaaaccgat
     8101 tgcccttgac ggcttacgag gcaagggcag gggggcgaag aagtgggtat ccggtgaagt
     8161 ggtgaaattt tgtttgatgt ggaaggtgaa gtgatgataa aaaagataat cttagatgct
     8221 tgttgcggta gccgaatgtt tcactttaat aagcacaacc cctcggtatt gtttgctgac
     8281 aatcgggaat ccctgtatcg aaaattttaa atgttatcga ataccaacct atctatgggc
     8341 ataaaagcgg taaacataac aaaacgcatt ggctggcatt tgtaaagatg gataataacg
     8401 attatttgta aagtatttca aaatacaaaa gccgataaat ttcattattt atcggctttt
     8461 gttttatggg ttgttttgct tgaattgttg aatagcttga ataatcagct ggttttgtgg
     8521 aatgttgagt ttttggctaa gtacttcgat ttctgcgatc acctcaagcg gtagtttaaa
     8581 ggatttttgc tttattcctc gttttgcctc gcttttagct tggatttcat ttcttgacat
     8641 tgccataatt tatccctatt ataaattttg ttgaagagct gggagctttc acccccagcg
     8701 agttgcttca gattagtaag ctggggagct aaggactaat aagaagacaa ctaggataat
     8761 gtatttaaac attgtgctat ccttcttcat ttcgtaaggt ttaagcctta cgctcacttt
     8821 caagctgtct cttgaaagtg aggttattat aatatagggt atcctacatt gcaagtaata
     8881 aatacaaaaa agccgataaa ttttcgttta tcggcttttg tttttattgc agtttgctca
     8941 caagttctga aatatcaggg ttatagtagg tattttgcaa aatacttaaa tctcgatgcc
     9001 ccgatacttt ggcaagctcc attaccgtca gatatttaga caaacgtgtg agtgcctctc
     9061 gccgtgtgtc gtgaaaatgc agatcggcat tgtgtagatt ggctttttgt ttcagcttgc
     9121 gaaaattcga atcaaggctg cgactggtga gctggaaaac actgttttgc ttgttcggtt
     9181 caagttgtgc caactgattt aaaattgcta ttgctttggt tgagagtggc acggtgcgtg
     9241 aataaccgtt tttggtttgt ggcaaaaatg cggtgcgtgc ctgcaggttt atatgttccc
     9301 attttaaacc agcaatttca cccgctcgca ttgcagtctc aatcgcaaat aatagtgctg
     9361 ctccggctct ttgttgcatt aatagcggtg gctggttagg gctgtagccg gagacaaaaa
     9421 tcagtttgtc tatctcctca tcgctatatc ggcgagttct tgctggtggc gttttgattt
     9481 tttccagcgt ttttagagga ttttctttga gataatccca ttccattgct tttgtaaaga
     9541 gtgccgataa agtggctctt tctcttgaga cggaaaccgg ctgcacttct tgcaagcgtt
     9601 ggttttgcca ttggcgtaaa tgctctttgg tgagtgccgg taatggtata tttcccaagt
     9661 tgctgccggc aattcttagc aggcgatagc gttcttccct tttgccacgt ttggtcggag
     9721 tgacctcttt gaggtatttg tcgattaatt cggcaaaaag aatgttcggg gtggtgttat
     9781 attctccggc atcaatttga gcctctaaag cgtaagccca gcggtttgcc tctgttttgg
     9841 tgctgaaagt ggcagtttta taaatgcctt ttttgcggac ttgtgctcgg tatttgttgc
     9901 cgttcttaat aatagtagcc atttttttat tcctttgtgc atgtgtgcag tttaagtgtg
     9961 caaatgtgtg caaaggaatg ttaatagaga tcataaacga tcaataaata tcaagttttc
    10021 atatcaaaaa tgatgttttt attgatgtta tgctacactt ggggaagtgt tttaaatcaa
    10081 taagctattg atttataagg ggtatttttg gggttaagcc tgaatgggga tattcaggct
    10141 taaaaataat ggtgcgacta gctggactcg aaccagtgac ccccaccatg tcaaggtggt
    10201 gctctaacca actgagctat agtcgcgtta acggtgtgca ttctattgga aaaaggaact
    10261 tagtgcaata tgcttttggt cgaatgcttt tattttagac gcttttgggt aaaaatttgt
    10321 ttatcttccc aacggagcat ggttaaatgg cttccccaag cacagccggt atcaagtgca
    10381 tagatgtttg gacgagtgca tttgcccatt aggcttgccc agtgaccaaa gataatgttt
    10441 ttctgtgtaa ataacggatt atctaagtca aaccaagcca gcagttcttg aggggcttgt
    10501 tcaagcggta atttacagtc aaaatctaac cgcttgtcgg tgtagcaaaa ccgcatacgg
    10561 gcaaacacat tgatgatata acgccaacgt tcaacacttt gcaaatcttc actccagcgt
    10621 tcgggcttat tctcatacat ttgggcgatg tagtccgcat aattgtcact ttgtagcacc
    10681 ttttctgcct ctttggcaca agctagggta gttgctaaat cccactcagg gctgattcct
    10741 gcgtgagtca gcaaaaattg atgtttgggg tgatgaatta caagcggttg atttctgagc
    10801 caattttgca aatcgagccg atctttagcc tcaaaaatag catcaatctt atcatttggt
    10861 tttacccgtt tgatgccaag cattgtggca agcaagtgca aatcgtggtt gcctagcacg
    10921 gttttggcat tgtttgcaga gtctttcaca aaacgtaaac aggctaaaga gtcctcacca
    10981 cgggcgacta aatcccctgt taaaaagagt tcgtcttgct tggggttata attgactttt
    11041 tctaataaaa gctgaaattc gttaaagcaa ccgtgtaggt cgcctacaat ataagttgcc
    11101 attgtttctc ctttgttccc ctaaagttat aaacaagcgg ttatatttta gagaaaattt
    11161 gcaaaatctc atcaaaatat aaccgcttga aatttaaatg atatttccta cgattgagcg
    11221 ggtttctaag cgtacttcgg tgagtttcac tttcaccgct tgcccgatct gataggcttt
    11281 ttcaccttta atataaagtg caagttcttc ttggcggtat tccatttcct ctttgttgtc
    11341 gtgcaagctg gagaatggca caaaaatttg agcaccgttt tttatgactt tcgcacgcaa
    11401 gccaccacgg gaaacatctg caatttcgca ctcaaattcg accgcttgtt ccaccattgg
    11461 ggcaaggtaa cgagcataaa gccagtcagc aatatcacgt tctactaaac ggttttgacg
    11521 gcgagcttct tgtaaacggc ttaaaactga ttcttccacc gcttggcttt gtttgccaag
    11581 taacacttgc ttgattaaac ggtggtttac catatcgcca tatttacgga tgggtgatgt
    11641 ccaagtggcg taatcggtaa tgcctagccc gaaatgcggg gcaacttcgg ctttaaattc
    11701 agcgaaggtt aaataacgtc gtaaacgcaa ctctaagaat ttctctgggt agtcttgaat
    11761 atcacgttgc atttggcaat agccttctaa ggttgctaaa cgctcggcgg agtagcgttc
    11821 ggcaagctca tcacgatttt catcgctggc taaagtttgg agtaaaaatg attttaccaa
    11881 ttcataattt ttcggatcaa aacctgaatg ggtattaaat acaccggttt tggcatgttg
    11941 gcttaagaat tttgcacaac aaatattggc aagaatcatt gattcttcga tcatttgatt
    12001 cgcaatacgg cgatattcaa cgtgaatatc ttttaccgag ccatcttcat ttaactcaaa
    12061 ggtataatcg ccttgttctt tgaatagcaa cgcattctcg gcacgccatt gaatgcgggc
    12121 ttgagtaaat tgatgtagcc actcaatttg ttgttttgtt tcttcagact gaggttgcca
    12181 cgcattttcc acgccttcga gataatcgga aacatcgtcg taagccaagc gatcttttga
    12241 ttcgacccaa gcagaggtaa aggttgtctc tttaatgaga ttgccgttta aatcggtttc
    12301 aataaagcca acgagggcag ggcgtttttc attcgggaca agcgaacaga gatcgtctga
    12361 gagttctcga ggtaacattg gaatgttaaa gcccggcaaa tagttggtga aacaacgctg
    12421 gcgagcggct ttttcaatat gggaattttc tgggatataa gcggttgggt cagcaatcgc
    12481 taccactaaa cgccagccgg ttcgcttgcc attttgctta attggttcga tgaacagagc
    12541 atcgtccata tcttgcgtgc ttgggctgtc aatagtggta aagtaaagat gagataaatc
    12601 ttcacgagct aattcatcgt gtaattcata gtttttttca tttgcgaccg gttcacgagg
    12661 ctgttcgtgg cgggcaagtg tgacccacca aggggcaaaa ttatcagatt ctttacagat
    12721 aaattgggta acttgagcaa agaaaaaacg gtcgtcacgc aatgggtgcg tttttaattg
    12781 tgcaaccacc cagtcgccat tttctaattt ttcagtgact tttttatggg tatttgccgg
    12841 gatagtttgc ttaatgcttg ggtgatcaac cgcaagttgc aatttgccct ctttattcaa
    12901 gcgaacttgg gcaataaatc tatccagcat tggctcaagt agtgaatcaa tttctacctg
    12961 ttctttgtcg ccatcacgtt tgactacggc tttcactgaa tcaccgtgca ttactttttt
    13021 catttccgca ggtggaataa aatagctttt tttatcgcat tctaaaaaac cgaatgcttt
    13081 gtctgaggct ttgacaacgc ctgcgacata ttctttgctt gcttcaattt gttgtttgag
    13141 ttgggctaaa aggggattgt tttgaaacat aatttctcat tttaaaaata aaaaaaggtg
    13201 ctatcgttgc accgaaaaaa acaatggcat tataacggat ttgaaccaag ttcagcaaat
    13261 aaatgctgtt gctataactt ataaaagtta gtagaatata atccatttaa aaataatgga
    13321 gacttttcaa tgaaaaaatt acttgcaatt gcaacagtat tttctttagc agcctgttct
    13381 actcaaacac ttgaactatc tcctgctaag gaagctcaat atgatcgtgt tcaacacttc
    13441 tttgtttcag ggtttgctca aaaacaagag atagatgcag caaaaatttg taatggtgca
    13501 gataaggtag gtaaggtaga aactgaaact actttcttta atggcttgtt aggtacaatt
    13561 acttatggta tctattcacc tcgccaaatc cgtgtatatt gtaactaatc ctagtaattt
    13621 aaggagttga aaatgaaaaa attgcttgct atgtctgtat taacagccct gctgactaca
    13681 gcatgtacaa cacaaactgc ccatattggc ggcaaagtta ctgataatat taaaccaact
    13741 aagagtgtaa ctcagtcgtt ctttataggt gggattgggc aagaagaaac attgaatgtt
    13801 gtagaggttt gtggttccaa agataaagtg cagcaggttg aaacagtact ttcaggtggg
    13861 gacattctat taggtcttgt gactatcggt atttatacac ctcgtactgc aaatgtatat
    13921 tgtaagtaat attttattaa aaaaataacg gactcaattg agtccgttat ttttatagga
    13981 attactcttc gtcgcctaat tcacccattg ccattacgct gaagccggca tcaacgtgta
    14041 acacttcacc tgttacacca gaggctaaat ctgagcataa gaaagctgca gagttaccta
    14101 catcatcaat agttacggtg cggcgtaaag cagcggtttt ctcgaaagca gaaagcattt
    14161 tcttgaagtt tttaatgcct gaagcagcta atgtacggat cgggccggct gaaatcgcat
    14221 ttacacgaat accttcttta cctaaatcgg ctgccattac acgggttgca gcttctaaag
    14281 aggctttggc taagcacatt acattgtagt tagggattgc acgctctgca cctaagtaac
    14341 ttaaggttaa aagggtggca ttttcattta agaaggggcg agcagcttga gccattgcaa
    14401 caaagctata ggcactgatg tcgtgtgcaa tacgataacc ttcacgggtt gcggcattta
    14461 cataatcgcc gtctaattga tcgcccggtg caaatgcgat agcgtgaata aaaccgtcaa
    14521 atttttccca atgtttgctt aattc
//
LOCUS       DANKBV010000054        14344 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.054, whole genome shotgun sequence.
ACCESSION   DANKBV010000054 DANKBV010000000
VERSION     DANKBV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.054"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1472)
                     /gene="mglA"
                     /locus_tag="PWY43_001326"
     CDS             complement(<1..1472)
                     /gene="mglA"
                     /locus_tag="PWY43_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDL2136205.1"
                     /translation="MTGEVLLTMTNVSKSFPGVKALDNANLTVKSHSVHALMGENGAG
                     KSTLLKCLFGIYAKDEGDILFLGKPVNFKTSKEALENGISMVHQELNLVKQVTVMDNL
                     WLGRYPLKGPFVDHDKMYRDTKAIFDELDIDVDPRDKVAKLSVSQMQMIEIAKAFSYN
                     AKIVIMDEPTSSLSEKEVEHLFKIIQKLKDRGCGIIYISHKMDEIFKICDEITILRDG
                     KWVNTVAIKDTSMDQVVSMMVGRELTQRFPEKTNLPKEVILEVEHLTAKNQPSIQDVS
                     FSLRRGEILGIAGLVGAKRTDIVEIIFGVREKAGGTIRLNDKEINNRNAFDAINNGFA
                     LVTEERRSTGIYANLSIEFNSLISNMKSYLGKFGLLSNKKMKSDTQWVIDAMNVKTPS
                     HQTHIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDIGAKFEIYQLILELANKDK
                     GIIMISSEMPELLGVTDRILVMSNGKVAGIVETAHTSQEE"
     gene            complement(1544..2533)
                     /gene="mglB"
                     /locus_tag="PWY43_001327"
     CDS             complement(1544..2533)
                     /gene="mglB"
                     /locus_tag="PWY43_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL2136206.1"
                     /translation="MKKAVLSAVAMAVALGATISTAQAENRIGVTIYKYDDNFMALMR
                     KEIEKEGKTQKVELLMNDSQNTQSIQNDQVDVLLSKGVKALAINLVDPSAYKTIIQKA
                     KSEGVPVVFFNKDPGAAAIDSYPKAYYVGTEPKESGIIQGELIAKQWKAHPELDLNKD
                     GKIQFALLKGEPGHPDAEARTKYVVESLKAQGIDSEQIFMDAALWDAAQAKDKVDAWL
                     SSGKAKDIEVIISNNDGMALGALEATKAHGRKLPIFGVDALPEALQLIKKGELAGTVL
                     NDGVNQGKAVVQLSANLAGGKDAVAGTQWKLENRVVRIPYVGVDKDNLSEFLK"
     gene            complement(2808..3800)
                     /gene="mglB"
                     /locus_tag="PWY43_001328"
     CDS             complement(2808..3800)
                     /gene="mglB"
                     /locus_tag="PWY43_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL2136207.1"
                     /translation="MKRSLLNAFVYTLMLGANASYAQSEPRIGVTIYKYDDNFMMLMR
                     KEMNKEMANFSHLKWFMNDAQNSQVKQLKQIETLLKHQVNVLAVNIVHANESKTIVEK
                     AKLSNVPVVFFNRSSDNQALESYDKAYFVSSDPKEAGRIQGELIAKVWKSNPDFDLNK
                     DGKLQFVLLKGEPSDVSAERSQAVVESLNQQGVQAEEIYSDTARWRRTLARNKMNEWL
                     VENRAAEIEVVISNNDEMAIGALDALNAHEKRLPIFGIDALPETLALMKKGEIAGTVL
                     NDSSAQAKAVVKLVSNLAQGKPALKDTQWESHSSKTIYLPHIGIDKENLGQFLP"
     gene            complement(3905..4308)
                     /locus_tag="PWY43_001329"
                     /pseudo
     CDS             complement(3905..4308)
                     /locus_tag="PWY43_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612952.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
     gene            4427..4714
                     /locus_tag="PWY43_001330"
     CDS             4427..4714
                     /locus_tag="PWY43_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL2136208.1"
                     /translation="MKNDKPIECEGCNTFDVGSILDNSETVANITRTYETEAEAQAAL
                     EIFTQKARDVESEPCEIESEIRPVDGGVKLNANFKFSCQAEVVIFQLGTRN"
     gene            4897..5892
                     /locus_tag="PWY43_001331"
     CDS             4897..5892
                     /locus_tag="PWY43_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2136209.1"
                     /translation="MLKPFKKLAAALAVTAAFSVSAFANDPIVIKFSHVVANETPKGQ
                     GALMFQKLVDERLNGKVKVEVYPNSSLYADGKEMEALLLNNVQMLAPSLAKFDKYTPK
                     IQIFDLPFLFDDIKAVERFEQSEAGQSLLKSMEDKGITGLSYWNNDLKQLSANKELRE
                     PKDARGLKFRVQASEVLDAQFKALKAVPRKMAFGEMYQGLQTGVVNGAENPYSNIYSQ
                     KIHEVQKFITASNHGLLSYMVIVNTDFWNKIPEDVRTELTKILDEVSVEVNKQSHDLN
                     QSDKQKIVDSGASQIITLTDEQREKWREAMRPVWKQFEDAIGADLIKAAEAANKQ"
     gene            complement(6029..6976)
                     /locus_tag="PWY43_001332"
                     /pseudo
     CDS             complement(6029..6976)
                     /locus_tag="PWY43_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621529.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7200..7823
                     /locus_tag="PWY43_001333"
     CDS             7200..7823
                     /locus_tag="PWY43_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2136210.1"
                     /translation="MSSLKWFWERFEECFIAILLMVMTLVTFTYVMFNNLYNVFFDLA
                     EAVPFTASFAEPLGEWIMEMAQEMTWSVALTKVCFGWLIFFGASYGVRTAGHIGVDVL
                     VKKLSRNAQKVVGLIAIAACLAYAFLIASASYEWISAMYVADIGAEDLHQFHLKQWHV
                     GLIMPVGFALIGIRFIEILVRILQGKQVGLGLADESEDAMKLAQGEE"
     gene            7823..9106
                     /gene="dctM"
                     /locus_tag="PWY43_001334"
     CDS             7823..9106
                     /gene="dctM"
                     /locus_tag="PWY43_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter large permease
                     protein DctM"
                     /protein_id="HDL2136211.1"
                     /translation="MIILVLFSLLFILMFLGVPIAISLGLAGAVTIMFFSQDSVSSLA
                     IKLFETSEHYTLLAIPFFLLAGAFMTTGGVAKRLIDFANATVGHIRGGLAIAAVMSCM
                     LFAALSGSSPATVAAVGSIAIAGMVRSGYPVGFGTGIICNAGTLGILIPPSVVMVVYA
                     ASTETSVGKLFMAGVVPGLMLGLVLMIAIYVVARIKNLPAQPRATVKEWFTAARKAIW
                     GLLLMVIILGGIYSGIFTPTEAAAVAAVYSWFVAVFIYKDVKLRDCPKVLLESAKLSV
                     MLMFIIANAMLFAHVLTTEQIPQLITNTVVEWNLQPWTFLLVVNIVLLIAGAFMEPSA
                     IILILAPILFPIAVQLGIDPIHLGIIMVVNMEIGLITPPIGLNLFVASAVSKMPLSDV
                     IKAAMPWLLLLLGFLVLITYIPAISMALPNWIGIN"
     gene            9287..10993
                     /locus_tag="PWY43_001335"
     CDS             9287..10993
                     /locus_tag="PWY43_001335"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDL2136212.1"
                     /translation="MSVSSFNRTWAKVILSALMRYGVKHFCIAPGSRSTPLTLEAVQL
                     QQTQYAECHTHFDERGLGFYALGIAKATNSPVAVIVTSGTAVANLYPAVIEASLSHQN
                     LIILSADRPTELIGCGANQAITQQGIFANYPAASINLPKANEAYNASWLVSTIEQACT
                     TQAQQGGVLHINAPFEEPLYEADINEILNTPWLRPIQGWLNNSQTKWIDQQAIQSEVS
                     MHENWDYWRTKRGVIVVGKLPPEQGTGLKNWAETMGWCLLSDVQSCIEASLPYADIWL
                     SNETVKQRLLQADIIIQFGTQIVSKRVNEFLKAYQGEFWLVDESQDYINPNAHHQTRF
                     VAKAHHFLRVHPPLRQKPWLLEPLALSQFCATFIEQQVGGSLSEASLAHNIQDILAIN
                     GNLFIGNSLFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGIAKGSDRPTVGVVG
                     DISALHDLNSIALLTKINQPTILFVVNNSGGAIFDMLPVESKAKDQYYRLAHNFEFAQ
                     VATMFGLEYIRPFTWADLKTKLKHAYARKGVTIVEIKVNEHDGSNMYKSLLKQISQAE
                     IA"
     gene            11042..11881
                     /gene="menH"
                     /locus_tag="PWY43_001336"
     CDS             11042..11881
                     /gene="menH"
                     /locus_tag="PWY43_001336"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDL2136213.1"
                     /translation="MLSYQWHAQQGTPVIFLHGLLGSQQDWQAVLQHLQNFPQIRPLT
                     IDLPFHGNSKHITCADFADLRQQLHATLESLIGSRPFYLVGYSLGGRAALDYVLNMDN
                     PNLQGVVLEGANIGLSTENDRQIRRQNDRHWANRFRQEPIEQVLEDWYKQPVFADLSD
                     TERECYIDSRKHNQGEQIAQMLEATSLAKQPDYTEKLKQTDKNIVFFIGEKDQKFRKM
                     ASSHQLPTQLIANAGHNAHRANAKNFVEKLGLLILHSDSDLRSQSVKHNGAALLHKSP
                     SGA"
     gene            complement(11930..13462)
                     /locus_tag="PWY43_001337"
     CDS             complement(11930..13462)
                     /locus_tag="PWY43_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDL2136214.1"
                     /translation="MTLAIVYSRASIGVEAPLVTIEVHISNGQPKLTIVGLPEATVKE
                     AGDRVRSALLNANFIYPPQRITINLAPADLPKEGGRFDLPIAIGILAASGQIDSDLLT
                     QFEFLGELALTGHLRGVHGTIPAVISADKAKRQMILAKQNANEASLVSNATTYFAGSL
                     LEVVNMLNKRDKLPICQHISQHSAEIRPLVSRDLTDIIGQQHAKRALMIAAAGQHNLL
                     FLGPPGTGKTMLASRLADLLPEMTDEEAIETASVTSLVQNELNFQNWKQRPFRSPHHS
                     ASMVALVGGGSIPKPGEISLAHNGVLFLDELPEFERKVLDALRQPLEAGEIVISRANA
                     KVQFPARFQLIAAMNPSPTGHYQGTHNRTSPQQVMRYLNRLSGPFLDRFDLSIEVPLL
                     PQGALQSGTENRGETTEQVRKRVFLARKMQFERAGKINSQLTTREIERDCQLSAQDAL
                     FLETALTKLGLSVRAYHRILKVSRTIADLAGEANIQQLHLAEALGYRAMDRLLQRLQG
                     EP"
     gene            complement(13594..13929)
                     /locus_tag="PWY43_001338"
     CDS             complement(13594..13929)
                     /locus_tag="PWY43_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136215.1"
                     /translation="MSCKTHANHEHEHGEGCGHTAIKHNDHIDYLHDGHLHHKHGDHY
                     DEHVLEVNETNPADCNAAHCCGDHVHGEGCGHEAVPHGDHIDYIVNGRLHHQHGDHCD
                     DHGPVEIVK"
     gene            14036..>14344
                     /locus_tag="PWY43_001339"
     CDS             14036..>14344
                     /locus_tag="PWY43_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2136216.1"
                     /translation="MKTELDMIVIGGGMIGAATALGLAKQGLRLALLEKNPLPTFNPD
                     SDYDLRISAISMGSIQLLEKLGVWEQIVAMRSTPYTQLETWEIDGFNTLFSAADLGLE
                     K"
BASE COUNT         4001 a         3167 c         2947 g         4229 t
ORIGIN      
        1 atttcttctt gcgaggtgtg agcggtttcg acaatacctg caactttccc gttactcatc
       61 actaaaattc ggtctgtgac cccaagcaat tccggcattt ccgatgaaat cataatgatg
      121 cctttatctt tattcgccag ctctaaaatc aactggtaaa tttcaaattt tgccccgata
      181 tcgataccac gggtcggctc gtcaagcatc aagatttcag gctgggttaa caaccaacgc
      241 ccgataatca ctttttgttg gttaccgccg gaaagtgagc cgatatgggt ttggtgtgat
      301 ggcgttttta cattcatcgc atcaattacc cattgggtat cgcttttcat ttttttattg
      361 ctcagtaagc cgaacttacc tagataagac ttcatatttg aaatcagaga gttaaactca
      421 atgcttaagt tggcataaat ccctgttgaa cggcgttctt ctgtaaccaa tgcaaagccg
      481 ttattaatcg catcaaaagc gttgcggtta ttgatttctt tatcgtttag tcggatagtg
      541 ccgcctgctt tctcccgtac accgaaaatg atttcaacaa tatcagtccg ttttgctcca
      601 actaagccgg cgatgcctaa aatttcccct ctgcgtaagc taaagctgac atcttgaata
      661 gagggttgat tttttgccgt taaatgctca acttcaagaa tcacttcttt cggcagattg
      721 gttttttcag ggaaacgctg ggtcagctca cgccctacca tcattgaaac cacttgatcc
      781 attgaggtat ctttaattgc aacggtgttt acccatttac cgtcacgcaa aatggtaatt
      841 tcatcacaaa ttttaaaaat ttcgtccatt ttgtgtgaaa tatagataat gccacagcct
      901 ctgtctttca gtttttggat aattttaaat aaatgctcga cttctttttc tgaaagtgac
      961 gaagttggtt cgtccataat cacaattttg gcattgtaag agaaagcttt tgcaatctcg
     1021 atcatctgca tttgtgaaac cgataatttg gctactttat cacgtggatc aacatcaata
     1081 tctaattcat caaaaatcgc cttagtatca cgatacattt tatcgtgatc tacaaaaggc
     1141 ccttttagtg ggtaacgacc tagccataag ttatccatta ccgtcacttg ttttactagg
     1201 ttcaactctt ggtgaaccat tgagatccca ttttctaacg cctcttttga ggtcttaaaa
     1261 ttcaccggct tgccaagaaa gagaatatct ccctcatctt tagcataaat cccaaataag
     1321 catttcagta acgttgattt acctgctcca ttctctccca ttaaggcgtg aacactatgg
     1381 gatttcaccg ttaagttagc attatctaag gcttttaccc cgggaaatga cttgctaaca
     1441 tttgtcattg tgagtaaaac ttcacctgtc atatctcacc cctgattaca tcgaaaaatc
     1501 gaaaaaaagc acgggtatcc atcacccgtg ctaaaagcgt gaattatttt aaaaattcag
     1561 ataagttgtc tttatcgaca ccaacgtaag gaatacgcac cacacggttt tccaatttcc
     1621 attgtgtacc agcaactgca tctttaccgc ctgctaagtt agcagaaagc tgaacaaccg
     1681 ctttaccttg gtttacacca tcatttaata ccgtaccggc taattcacct tttttgatta
     1741 attgaagtgc ttctggcaat gcatctacac cgaagattgg taattttcta ccgtgagctt
     1801 tggttgcttc taatgcacct aacgccatac catcattatt agaaataatt acttcgatgt
     1861 ctttcgcttt accacttgat aaccaagcat ccactttatc tttagcttgg gctgcatccc
     1921 ataatgctgc gtccataaag atttgctcac tgtcgatacc ttgtgctttt aagctttcta
     1981 ccacatattt agtacgtgct tccgcatcag ggtgacccgg ctcacctttt aataaggcaa
     2041 attggatttt accgtcttta ttcaagtcta attcaggatg ggctttccac tgttttgcaa
     2101 ttaactcacc ttgaataatg cctgactctt tcggctcagt acctacataa taagctttcg
     2161 gatagctatc aatcgcagct gcacccgggt ctttattgaa gaacaccaca ggcacacctt
     2221 cactttttgc tttttgaata attgttttat aagctgatgg atccactaag ttaattgcta
     2281 atgctttaac ccctttagaa agtaatacat caacttggtc gttttgaata gattgcgtat
     2341 tttgcgagtc attcattaat aactcgactt tttgtgtttt accctctttt tcgatttctt
     2401 tacgcattaa tgccataaag ttatcgtcat atttataaat tgttacacca atacggtttt
     2461 cagcttgagc tgtactgatc gttgcaccta atgcgactgc cattgccact gcacttaata
     2521 ctgctttctt cataggagat cacctctttt atgtgtttat agggaaatga attgcttacc
     2581 ataagcaact tggtgcaaag tataactaat tttaaaaaaa caatccgtga tcattctaac
     2641 agaattaaga caaagtggag aaatttttaa tttgaagtat aaaatttgaa tattcgctct
     2701 attcaaatct gttggaagaa aagagaaagg atttgcaaga ttttactgaa aaatgaccga
     2761 ttgttatcat caataagggg cgtatttata acgccccgct cctccattta aggtaaaaat
     2821 tgccctaaat tttccttatc aataccaata tgtggaagat aaatcgtttt agaggaatgg
     2881 ctctcccact gcgtatcttt cagagcaggc ttaccttgtg ccaaattaga taccaatttc
     2941 accaccgctt tcgcttgtgc agagctatca tttaataccg tgccggctat ttcccctttt
     3001 ttcattaatg ctaacgtctc aggcaaggca tcaataccaa agatcggcaa tcttttctcg
     3061 tgtgcattta acgcatctaa agcaccaatt gccatttcat cattgtttga aatcacaact
     3121 tcaatctcag ctgcacggtt ttccaccagc cactcattca ttttattgcg agccaatgtt
     3181 ctacgccatc tcgccgtgtc actgtaaatt tcttctgcct gaacaccttg ttgattaagc
     3241 gattcaacca cagcttgaga acgctcagcg ctcacatcac tcggctcgcc cttaagcaat
     3301 acaaattgca atttcccgtc tttatttaga tcgaaatcgg ggtttgattt ccacactttc
     3361 gcaattaatt ccccttggat tctaccggcc tcttttggat cactactcac aaaataggct
     3421 ttgtcataac tctctaatgc ttggttatca gacgagcggt taaaaaacac cacaggcaca
     3481 ttagacaatt tggctttctc tacaattgtt ttcgattcat tcgcgtgaac aatattgact
     3541 gccaatacat taacttgatg cttaagcaac gtttcaatct gctttaactg ttttacctgc
     3601 gaattttgag catcgttcat aaaccatttg aggtggctga aattcgccat ttccttgttc
     3661 atttctttac gcatcaacat cataaagttg tcatcatact tataaatcgt aacgccaatt
     3721 ctaggttcag attgagcata gctcgcattc gccccaagca ttagcgtata gacaaaagca
     3781 tttaataggc tacgtttcat cgttttcata gaatgtccct cttcctatgt tgaaatacgt
     3841 tagcgaaagc atagcgaatt tagcaaagcg aaaccgtgat cggcttcaca gattagaaag
     3901 attttcattg gttcgatgta ataaatttca ggcgtaaatg gtcataaatc caattaaaaa
     3961 taatggaata aaccagcaca aataatgtca taccaatatc cattacaaag gcttctcacc
     4021 agccaatatt aagcatatac attaccaatg gaatggtaaa aaccaacagc ccgccttcaa
     4081 ataagaccga atgcccaatc ttcttgcaat tcgctcgccc ttgactactt tatcaaacag
     4141 ccagttatag acaaaattcc acacccttgc gacaacagag atcataataa tcacactcgc
     4201 taacgagctt gtgctatgtt ccgtgagcag tgatgttaaa atcacggtaa acacaatggc
     4261 aaaaaactca aataaaactg catgtagcac acgctcccaa gcggtcattt ttcctccaaa
     4321 atttgcaaaa ggctatctaa atcaaattat cgtgaaatca cgattgcacc cacgcttatt
     4381 tagttttaaa gtagtgatat tatttatcaa ttatgaggat tagccaatga aaaacgataa
     4441 acccattgaa tgcgaaggct gcaatacctt tgatgtcggc tcaattttag ataatagtga
     4501 aactgttgca aacattactc gcacttacga aaccgaagcc gaagctcaag cagcattaga
     4561 aatatttact caaaaagcac gtgatgttga atccgagccg tgcgaaattg agagcgaaat
     4621 ccgccctgtg gacggcggcg ttaaactcaa tgctaatttt aaatttagct gccaagctga
     4681 agtagttatc ttccaattag gtacaagaaa ttaatagaaa ggctggttta accagccttt
     4741 cttctcttta aaagcactcc atttcactcc aaagtaacaa taatgtaaaa aattgagata
     4801 tatgtcatat ttttaaaaat ttgttgttta cttttagtaa aaatcaggca aattatgaac
     4861 aactttgcaa ccactaacta tttggagtaa attattatgt taaaaccatt caaaaaattg
     4921 gctgcggcat tagctgtaac tgctgcattc tcggtatctg cttttgctaa tgacccaatc
     4981 gtcatcaaat tctcacacgt tgtagcaaac gagaccccga aaggtcaggg tgcgttaatg
     5041 ttccaaaaat tagtggacga acgcttaaac ggcaaagtga aagtggaagt ttatccaaac
     5101 tcttcacttt atgcagacgg taaagagatg gaagcattat tgttaaataa cgtacaaatg
     5161 cttgctccat cactggctaa atttgataaa tacacaccaa aaattcaaat tttcgatcta
     5221 ccgttcttgt ttgacgatat caaagcggtt gaacgttttg agcaatctga agcaggtcaa
     5281 tcattgttaa aatcaatgga agacaaaggt attacaggtt tatcttactg gaacaatgat
     5341 ttaaaacaac tttctgcgaa caaagagtta cgtgagccga aagatgctcg tggtttaaaa
     5401 ttccgtgtac aggcttcaga agtgctagat gcacagttca aagcattaaa agctgtaccg
     5461 cgtaaaatgg cattcggcga gatgtatcaa ggcttacaaa caggcgttgt aaacggtgcg
     5521 gaaaacccgt attcaaacat ctactcacaa aaaattcacg aagtacaaaa attcattact
     5581 gcatctaatc acggcttatt atcttatatg gttatcgtga acaccgattt ctggaacaaa
     5641 attcctgaag atgtgcgtac agaactaacc aaaatcttag atgaggtgtc tgtggaagtg
     5701 aacaaacaat ctcacgatct caaccaaagc gataagcaaa aaatcgtcga ttcaggtgct
     5761 tcgcaaatca tcactttaac tgatgagcaa cgcgaaaaat ggcgtgaagc aatgcgccct
     5821 gtatggaagc agtttgaaga tgctatcggt gcagatttaa tcaaagcggc cgaagcggca
     5881 aataaacagt agtctcttta tctattgttc ttttgagcga atgcggtggc tttttaggct
     5941 cgtcgcattc ttatttttat tgataatgtg aaatgatgtg tagcaactgt atctccccaa
     6001 catttttggc aagatacaaa ttcgttaatt aaaccgcgtg agcattccac tgtgagccat
     6061 cacggacgat ggcattaatc acggttaaaa gcttacgcat acaggcattt accgccactt
     6121 tgaacggttt gccacgagca atcagccgtt cataaaatgc cttgaacttc ggttcgtgtt
     6181 ggcgggcgac attcgccgca ttataaagta cgtttcgtac cgcttgtcgc ccacccgaac
     6241 agcgactatg ccatttctgg ctaccgctct ctttcgggag tggggcaaca ccaaccagtg
     6301 aagcgatttg cttgtggttg agcttgccga gttcagggag cattcacatc aaagttgcac
     6361 tcgtgattgc acctatccct ttgatgtcat tgaacttttt taaagtgtcg tcaaatacag
     6421 cagtcgcttg attgatttgt gtttccagct catcaattaa acggtcaaaa tgggcaatca
     6481 actgttggac actttcccgc tggctgtcgt ggacttgatg aaggcggttt ttctccgcca
     6541 cccgcatttc aaccagttgg gtacggcgta aaactaatgc ttctaattgc tccatttcag
     6601 gtgttggcgg aagataaagt tgtttttcaa cctcatcgtc caaaaccagc attttggcgt
     6661 aagatgccag catttttgca tcttttgcat cggttttcgc caacgattta gccattgcaa
     6721 actgatgtgt ttggcgtgga ttagcaatca ccacccgaaa atccgcacga tgaagtgctt
     6781 tggcgagtgg gatttccaag ccgcctgtgc tttctaagac aactaggctt agatttgcac
     6841 aatttttcag gtattccacc gcttgttgga tacctttggg gttgttggta aaagttttgg
     6901 tctgttttcg actgctgata ccgacaacaa aactacgttt agcaacatca atgccacaga
     6961 aattaagttc attcatcgta acctgccttg tattcgggtt gtgttcgtgc aactgttcgg
     7021 tttttgtgat gaaagttatc ggctctctaa gctacacaac gaagtgcttt caagggacga
     7081 gacgagatgc cgataacggg ttgtgttgct tagtttaact catttgagat atacaagggg
     7141 cgggcacaag acccgcccct acgagaaaac atcgatacca catttttatg gagtgccaaa
     7201 tgtcttcttt aaaatggttc tgggagcgtt ttgaggagtg ttttatcgcc attttgctaa
     7261 tggtaatgac gcttgttacc ttcacttatg tgatgtttaa caacctttat aatgtcttct
     7321 ttgatttagc cgaagctgtg ccgtttaccg cgagttttgc tgagccgtta ggcgaatgga
     7381 ttatggaaat ggctcaagaa atgacttgga gcgtggcttt aaccaaagtc tgctttggtt
     7441 ggcttatctt ctttggtgcg tcttatgggg tgcgtaccgc agggcatatc ggagtcgatg
     7501 tattggtgaa aaaactctca cgcaacgccc aaaaagtcgt aggattgatt gcgattgccg
     7561 cctgcttagc ctatgccttc ttaattgcct ctgccagcta tgagtggatt tcagcgatgt
     7621 atgtggcaga tattggagca gaagatttac atcaattcca cctcaaacag tggcacgttg
     7681 gtttaattat gcctgtcggt tttgccttaa tcggtatccg ttttattgaa attttagtgc
     7741 gtattttaca gggcaaacaa gtgggcttag gcttagcaga tgaaagtgaa gacgcaatga
     7801 aactcgcaca aggagaagaa taatgataat tttagtgcta tttagcttgt tatttatctt
     7861 aatgtttctt ggcgtgccga ttgcgatttc actcggttta gctggtgctg ttactattat
     7921 gtttttcagc caagattctg tcagctcctt agccatcaaa ctatttgaaa cctcagaaca
     7981 ctatacgctc cttgcaatcc cattcttctt gcttgcaggt gcgtttatga caactggcgg
     8041 tgtagcaaaa cgcttaattg atttcgctaa tgcaaccgtc ggtcatattc gtggtggttt
     8101 agctattgcg gcggtaatgt cttgtatgct attcgctgct ctttcaggct caagccccgc
     8161 aacggtagca gcagtaggtt cgattgccat tgcaggaatg gtgcgttcag gctatccggt
     8221 cggttttggt acggggatta tctgtaatgc aggtacactt ggtatcttaa ttccgccatc
     8281 ggtagtaatg gtggtatatg ccgcttcaac tgaaacctca gtcggtaaat tatttatggc
     8341 aggggttgtg ccgggcttaa tgcttggctt ggtattgatg attgctatct acgttgtcgc
     8401 tcgtattaaa aacttaccgg cacagcctcg tgcaacggta aaagagtggt ttaccgccgc
     8461 ccgtaaagcg atttggggct tattgctgat ggtgattatc ttaggcggta tttacagcgg
     8521 catcttcacc ccaaccgaag ctgcagcggt agcagcggtt tactcgtggt ttgtggctgt
     8581 ctttatctat aaagatgtca aactgcgtga ttgcccgaaa gtcttgctgg aatccgccaa
     8641 attaagcgta atgttgatgt ttatcattgc caatgcaatg ttattcgccc acgttctgac
     8701 gaccgaacaa attccacagc tcatcactaa tacagtggtt gaatggaatt tacagccgtg
     8761 gacattccta ttagtggtaa acatcgtgct attaattgcg ggggcattta tggagccgtc
     8821 tgcgattatc ttaatccttg ccccaatttt attcccgatt gcggtacaac tcggcattga
     8881 cccaatccac ttagggatta ttatggtggt gaatatggaa atcggcttga tcaccccacc
     8941 aatcggcttg aacttattcg ttgcctctgc ggtatcgaaa atgccactgt ctgatgtaat
     9001 taaagccgca atgccgtggc tattactgct attaggcttc ttggtgttaa tcacctacat
     9061 tcctgctatc tcgatggcgt taccgaattg gattggaatt aattaataca agcggttatt
     9121 ttttgtaaat tttttacaaa tctatgatta ccgcccaatg ctactcatca gcattgggcg
     9181 gtaattttat taaaaatcac ctattttgct aaaaacttac caaaataatt taactttttg
     9241 ctacaatttg ggctatttat cgaattatta atgagtagag aagaatatga gtgtaagttc
     9301 ttttaatcgc acttgggcaa aagtgatttt aagtgcgtta atgcgttatg gcgtaaagca
     9361 tttttgtatc gcaccgggtt ctcgttccac cccattaacc cttgaggcag tacaacttca
     9421 gcaaacccaa tatgcagaat gccacaccca ctttgatgag cgtggtttag gcttttatgc
     9481 gttaggcatt gccaaagcaa ctaatagccc tgttgcggtg attgttacct ccggcacagc
     9541 ggttgctaat ctttaccctg cggtaattga ggcaagcctt agccatcaaa acctgattat
     9601 tctttctgca gatcgcccaa cagaattaat tggttgtggt gcaaaccaag ccattactca
     9661 acaaggcatt tttgcaaatt atcctgctgc gagcataaac ttaccaaaag caaacgaggc
     9721 ttataatgca agctggttag tttcaaccat tgaacaggct tgcactactc aagctcaaca
     9781 aggaggcgtg ttgcacatta atgccccttt tgaagagccg ctttatgaag ctgacattaa
     9841 cgaaattcta aatacccctt ggttacgccc aattcaaggt tggttgaata attcccaaac
     9901 caaatggatt gaccaacagg caattcaaag tgaagtctcg atgcacgaaa attgggatta
     9961 ctggcgaacc aaacgtggtg ttattgtggt aggcaaatta ccgccagagc aaggcacagg
    10021 cttaaagaac tgggcggaaa caatgggctg gtgcttatta agcgatgtgc aatcttgcat
    10081 tgaagcaagc ctaccgtatg cggatatttg gctctctaac gaaaccgtga aacaacgttt
    10141 attgcaagcc gatatcatca ttcaattcgg cacacaaatt gtcagcaaac gagtgaatga
    10201 gttcttaaaa gcctatcaag gcgaattttg gctggttgat gagtcgcaag attacattaa
    10261 cccgaacgca catcatcaaa ctcgttttgt tgctaaagca caccacttct tgcgtgttca
    10321 cccaccactt cgccaaaagc cgtggttgct tgaaccctta gcactttcac aattctgtgc
    10381 gacctttatt gagcagcaag tgggtggaag tttaagtgag gcatcacttg cacataatat
    10441 tcaagatatt ttagcaatta atggtaatct attcatcggc aatagtttat ttgtgcgttt
    10501 agttgatgcc ttatgcaaat tgcctgaagg ctacccggtt tacactaatc gtggggcaag
    10561 tggcattgat ggcttaattg ccacaatggc aggcattgca aaaggtagcg accgcccgac
    10621 tgtcggtgtg gtaggtgata tttccgcatt acacgatctt aactcaattg cattgctcac
    10681 caaaattaac cagccgacca ttttatttgt ggtgaataac agcggtgggg caatttttga
    10741 tatgttaccg gttgaaagca aagcgaaaga tcaatattac cgcttggcac ataactttga
    10801 atttgcacaa gtggcgacaa tgtttgggct ggaatatatc cgtccgttta cttgggctga
    10861 tttaaaaacc aaactcaaac acgcctatgc tcgtaaaggt gttaccattg tggaaatcaa
    10921 agtaaatgaa cacgacggca gcaatatgta taaatcattg ctaaaacaaa tttcacaggc
    10981 ggaaattgcc taatcacaac tgcccctcgc caatcctgcg aggggtaaaa atgtatcgac
    11041 tatgctttcc tatcaatggc acgctcaaca aggcacacct gttattttct tacacgggct
    11101 actcggctca caacaagact ggcaagctgt tctccaacat ttgcaaaatt ttccgcaaat
    11161 tcgaccgctt accattgacc ttccttttca cggaaatagt aagcacatta cctgtgccga
    11221 ttttgccgac ctgcgacaac aactacacgc cacccttgaa agcctaatcg gctcacgacc
    11281 tttctattta gtcggctact cactcggtgg acgagcggca ctcgattatg tgctaaatat
    11341 ggataatccg aatctgcagg gcgtggtgtt agaaggggcg aatatcggct taagcacaga
    11401 aaacgaccgc cagattcgcc ggcaaaatga ccgccattgg gcaaaccgtt tccgtcaaga
    11461 acccatcgaa caagtgcttg aagattggta caaacagcct gtttttgcgg atttaagcga
    11521 tacggaaaga gaatgttata ttgatagcag aaagcacaat caaggagagc aaattgctca
    11581 aatgctggaa gccaccagcc tcgccaaaca gcctgactac accgaaaaac tcaagcagac
    11641 ggataagaat attgtctttt ttatcggtga aaaggatcag aaatttcgca aaatggcatc
    11701 aagccaccaa ctaccaacac aactgattgc taatgcaggg cataacgctc atcgggctaa
    11761 tgctaaaaat tttgtggaga aattggggtt attgatactc cactccgaca gcgacctacg
    11821 gtcgcaatcg gttaagcata acggtgcagc tctgctgcac aaaagcccca gtggggctta
    11881 atcagccgta ggctaaatat aacctagtac ggctcagccg tgctaggtgt tagggctccc
    11941 cctgcaaccg ttgcaataag cgatccatcg cccgataacc aagtgcttcc gctaaatgca
    12001 gttgttgaat attcgcctcg ccggctaaat cagcaatagt ccgagaaact ttcaaaatac
    12061 ggtggtaggc tcgtaccgat aaaccgagct ttgtcaatgc agtttctaaa aatagggcat
    12121 cttgtgccga taattggcaa tctcgctcaa tttctcgggt ggtgagttga gaattgattt
    12181 tacctgctcg ctcaaattgc atttttctgg ctaaaaacac ccgcttgcga acctgctcgg
    12241 tggtctctcc tcgattctcc gtcccacttt gcaatgctcc ttgtggcaaa agtggcactt
    12301 ctatagagag atcgaaacgg tctaagaaag gtccggaaag gcggtttaaa taccgcatta
    12361 cctgttgggg cgaagtgcgg ttatgcgtgc cttgataatg ccctgtcggg cttgggttca
    12421 tcgctgcaat taattgaaag cgtgccggaa attgtacttt agcgttggct cgggaaatca
    12481 caatttcgcc tgcctccaac ggctgacgta acgcatcaag cactttacgt tcaaattcag
    12541 gcaattcatc taagaacagc acaccgttat gggcaagtga aatttccccc ggtttgggga
    12601 tcgagccacc tcccactaat gccaccattg atgcactgtg atggggcgaa cgaaacgggc
    12661 gttgtttcca gttctgaaaa ttcagctcat tttgcacaag gctggtcacc gaggcggttt
    12721 caatcgcctc ctcatcggtc atttccggca gtaaatccgc cagccggctt gccaacattg
    12781 ttttacccgt tcccggtggt ccgagaaaca gcaaattatg ttgcccggca gcggcaatca
    12841 ttaaggctcg cttggcgtgt tgttgcccga taatatcagt aagatctcgg ctgacaagcg
    12901 ggcgaatttc ggcagaatgt tgcgaaatgt gctgacaaat cggcagttta tcccgtttgt
    12961 tgagcatatt taccacctcc aacaacgagc cggcaaagta ggtggtggca ttggaaacaa
    13021 gcgatgcctc attcgcattt tgcttggcta agatcatctg ccgtttggct ttatcggcag
    13081 aaatcactgc cggaatcgtg ccgtgcactc ctcgcaaatg ccctgtcagt gccaactcgc
    13141 ccaaaaactc gaactgcgtt aataaatcag aatcaatttg ccctgaggct gccagaatgc
    13201 caatcgcaat cggcaagtca aaacgtccgc cttctttggg taaatctgcc ggagcaaggt
    13261 taatcgtgat ccgttgtggc ggatagataa aattggcgtt caacaacgca ctccgcacac
    13321 gatcacctgc ctctttcact gtggcttcgg gtaagccgac aatggttaat tttggttgtc
    13381 cgttgctgat atgcacttca atcgtcacca acggggcttc aacgccaatg gaggctcggc
    13441 tgtaaacaat cgctaaggtc atcacatttc ggtgttagaa atcaatgccg agagcataaa
    13501 aggaaaatcg gaagaggggt agcagcaatg ttcaattttt cgagcgagat cgcaaaaata
    13561 ataatgggag gctaaaacct cccatcaaaa accttatttc acaatttcaa ccggcccgtg
    13621 gtcatcgcag tggtcgccgt gttggtggtg caaacgcccg ttcacgatgt agtcgatatg
    13681 atcaccgtgc ggcacagctt cgtgtccgca accctcacca tgaacgtggt cgccgcagca
    13741 atgggcggca ttacagtctg ccgggttggt ttcattcact tctaaaacgt gttcgtcata
    13801 gtgatcgccg tgtttgtggt gtaaatgccc atcgtgtaaa tagtcgatat ggtcgttatg
    13861 tttaatagca gtatgaccac agccttcgcc atgttcgtgc tcgtggtttg catgagtttt
    13921 gcaagacata aaacgctcct tagataaata gataaatcgg tttgatccta atctgaaatg
    13981 cactacaatt caaggcgttt tagcagtatt ttgcctaact aaaaaggaag aaaaaatgaa
    14041 aactgaatta gatatgattg taatcggtgg cggtatgatt ggggcagcaa cagctctagg
    14101 cttagcaaaa caggggctgc gtttagcctt gttggagaaa aaccctctgc cgacctttaa
    14161 tcctgattca gactacgatt tgcgaatttc cgccattagt atgggctcga ttcaactgtt
    14221 agaaaaattg ggcgtgtggg agcaaattgt tgcaatgaga agcacccctt atacccagct
    14281 cgaaacctgg gaaattgatg gattcaacac tcttttttcg gcagctgatt tgggtttgga
    14341 aaag
//
LOCUS       DANKBV010000055        14327 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.055, whole genome shotgun sequence.
ACCESSION   DANKBV010000055 DANKBV010000000
VERSION     DANKBV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14327
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.055"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            25..159
                     /locus_tag="PWY43_001340"
     CDS             25..159
                     /locus_tag="PWY43_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/trimethylamine N-oxide reductase
                     NapE/TorE"
                     /protein_id="HDL2136217.1"
                     /translation="MRSELLKTLLLSGLILPLLMAFAVGAYGFIVWMLQLFVIGLPGA
                     "
     gene            163..1326
                     /gene="torC"
                     /locus_tag="PWY43_001341"
     CDS             163..1326
                     /gene="torC"
                     /locus_tag="PWY43_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDL2136218.1"
                     /translation="MLGSIKALFTRPATKIGLGVLVGIGFIAGAISWHQFNKAMDHTS
                     TEEFCVSCHSMQQPLEELKKTAHWSNKSGVTATCSSCHLPHDYTEKYARKVQASREVW
                     AELSGKYKEEGSFEKHRLEMAEREWARFAANGSKECKACHSYERMNFDKMSEAARKAM
                     KPAAEKDQSCMDCHKGIAHHLPKADENALKSKFDQFVNKSPSQGKSYYTKGNAELFLD
                     EGLSQTIGQLETAVPVAFVQAGKNADLVELTMWRKEKGFGRIWYHDFGKNITDAVLTK
                     EFMANQPKFEVLETKEDPITGLNWQKVKLQAWIGKAQLLDDVTPIWANAENQYKTQCS
                     TCHRQPDVAHFDSNSWIGLFNGMVGFTNMDKQTGKEVLRYLQMHASDSEEAKH"
     gene            1426..3891
                     /gene="torA"
                     /locus_tag="PWY43_001342"
     CDS             1426..3891
                     /gene="torA"
                     /locus_tag="PWY43_001342"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL2136219.1"
                     /translation="MQQSRRQFLKNMSVMAAAVTMPNFLVPRNVFASEDLSQWKVSGS
                     HWGAMRAKIENGRVAEIKPFEFDKHPTEMLNGIKGLIYSDSRIRYPMVRLDWLKNRHN
                     SNTAQRGDNRFVRVTWDEALDLFYQELERVQKEYGPWALHTANVGWRSTGQFHSCGNH
                     MIRAIAMHGNSVGTAGDYSTGAGQTILPYVLGSTEVYSQGTSWEIILKESENIIFWAS
                     DPVKNLQVGWNCETHEAYAYLEKLKEKVAAKGVNVICVDPVKSKTQNFLGCEQQYINP
                     QTDVPFMLALAHTLYTENLYDKKFLDMYTVGFEKFLPYLLGETEDKVAKTPEWAAEIC
                     GISADKIREFARMLAGKRTQFIFGWAIQRQQHGEQPYWMGAVLASMLGQIGLEGGGIS
                     YAHHYSSIGIPESGAAMPGAFPLNIDEGQTPKYDNKDYKGYSDTIPVARMTDSLLHAG
                     ETIDYNGKKVTYAPFKMAIFTGCNQWHRHSERNKMKQAFQKLETIVSINYSWTATCRF
                     SDIVLPACTPFERNDIDAYGSYSNRGVIAMHKLVDPLYDSRSDFEIFRDLCRRFGKEK
                     EYSRGMDEMQWVEKLYKDCRRENRGKFEMPEFAEFWQKGYVLFPEGKPWVRHADFRED
                     PELHALGTPSGFIEIYSNKIASFGYDDCKGHPMWFEKAERSHGGKNSDKFPYWLQSVH
                     PDKRLHSQLCESKELRETYSVQGREPLYINPQDAAKHGIQDGDLVRVFNDRGQAIVGA
                     VLSDNFPTGVVRLQEGAWYSPLDESVGAIDTYGDPNTMTLDIGTSKLAQAVSANTCLV
                     NIEKFIGNAPAPNGFNGAIEVTA"
     gene            3891..4481
                     /gene="torD"
                     /locus_tag="PWY43_001343"
     CDS             3891..4481
                     /gene="torD"
                     /locus_tag="PWY43_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDL2136220.1"
                     /translation="MTLAISREERQFIYGWFNAMLARELSDEQLNALQAGEFDDFFAY
                     LSELGFAEQVAKFQAEVTACRQLEFPRLELAADFAQLFLLEGNLSAIPYASAYLSGEA
                     LAKNLAEMDRLLGEFGLQVNRETKEPSDHLCVYLTLFDRLLAEKPLETQQQFLQNQLM
                     SWLPAWFAKAKTVKTNTGVYPALLRLFEQVLLKEQR"
     gene            4621..5310
                     /locus_tag="PWY43_001344"
     CDS             4621..5310
                     /locus_tag="PWY43_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2136221.1"
                     /translation="MQNNKTTYLNWYDLCVLTLILFGEAIYTSTQQYFITTEMQGNFE
                     ENLTFSNEQNYWALLTQLALLLIAMGYLKWRNFDFSRWVIRPSWLAFFSGIGLFILLS
                     LAMDLYFETLYGVYNLVYPEPFWAVLREVNISTVLYAILNGFYEEIFFLGICLAVKPE
                     YLKWAVIYSLIIRVSFHTYQGMESALGLGIWLGLIIYLLYSRSKQKNLFPFWVAHSIA
                     DILGLSLLAYF"
     gene            5468..6221
                     /locus_tag="PWY43_001345"
                     /pseudo
     CDS             5468..6221
                     /locus_tag="PWY43_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            6270..7664
                     /locus_tag="PWY43_001346"
     CDS             6270..7664
                     /locus_tag="PWY43_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL2136222.1"
                     /translation="MYKKHFLFLLVSPLVMAETVEFQQDPKEFDGQRQAVKFEQEIAK
                     KDLKLTAYLEEDNSLKGIEQHINQAILRQDWAKLERLLGDYAKQEQFDPLLLDYALAA
                     LAYAKKDYRSAAFYYQQLLKKQPDLTYPRFDLALILKEDYRYREAEQEFKQLKTDDLR
                     NAVNSALAQIEEEESWKPDFYLQYTQTNNVNNASSSPTVNVNGRIFIKESDSLPQSAK
                     GVRYGVGLSKMRNIGGNHFVGVDLGYNGVYYWNNHDYSEQSVSLTPKYSYRTATYRIG
                     VSPFIEQNWLGSSRYNYQFGSSINAVKLFERGLISGSFSHLQKRYSEQHIADRYNGYR
                     NQLTLGTQWQAVKNWRFFANLNASREITREKAQSSNKWLATIGVIYQTPSWASSVSVG
                     YGKRYFADKHYLFGYKRQDKEYQANVAVWNRSWHWHGIVPKLNFRYQKIDSNIADFYS
                     RSNKEVFMTLEKLF"
     gene            complement(7716..8336)
                     /gene="nudF"
                     /locus_tag="PWY43_001347"
     CDS             complement(7716..8336)
                     /gene="nudF"
                     /locus_tag="PWY43_001347"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDL2136223.1"
                     /translation="MKLTQYQHNDLQILNRETVYKGHFELQKITFRHKLFSGEMSGEV
                     VRELLIKGEAAALIAYDPILDKVVLIEQVRIGAYDAGQKKSPWLLELVAGMVDKGNEE
                     PAEVAMREAKEEAGLEVTQVQHALSVWDSPGGQLERLHLFLGLIDNTKVKSGEVHGLL
                     EEENEDILVHVVSREQAYQWVEDGTIDNVIAVIGLQWLQLNYQKFN"
     gene            complement(8345..8764)
                     /gene="psiE"
                     /locus_tag="PWY43_001348"
     CDS             complement(8345..8764)
                     /gene="psiE"
                     /locus_tag="PWY43_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDL2136224.1"
                     /translation="MPKKSLEEHHSSFGKIASETFQWIMNIALLVTGALLSYSLFAEA
                     YSLYELSFSDSEKFQIVEKIVIFFLYFEFLALIVKYFKSNYHFPLRYFLYIGITAMVR
                     LIIVDHSNALHTLLFALSILVMIIALYIVHTDRLHKS"
     gene            complement(8774..9496)
                     /locus_tag="PWY43_001349"
     CDS             complement(8774..9496)
                     /locus_tag="PWY43_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDL2136225.1"
                     /translation="MQNFANIEIAFIHRKAPLPSDFSYPEMPGGLTEKQVQKWQSRRV
                     AFFLLTELFKKHHLDLDLLADMQRSPSGRTFVDSEQIDFNISHSGEWIAVIFSYSFAK
                     LAVGIDIEHPQKIRRYHDLICHYGNAEEQNVLLANHSPILSNLAERFYLSWCLREAIL
                     KSQGVGIVKLSEVKHLPLEKQIFSAHCPKGKLHFVSELPFYLSYFYQQPQNMLLSEPL
                     LYHWQNGQFQPIKCQSLVYDVN"
     gene            complement(9502..9930)
                     /locus_tag="PWY43_001350"
     CDS             complement(9502..9930)
                     /locus_tag="PWY43_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL2136226.1"
                     /translation="MTHDWLYWALASALFAALTAIFAKVGLQGVDSDFATFIRTVVII
                     IALIAFLTYSGKWQPLDSLSSKNWLFLILSGLATGLSWSAYFKALQMGNASQVAPIDK
                     FSLVLVTLFAVIFLGERPSGQDWLGIGLVAGGVLLLAFKR"
     gene            10098..11288
                     /gene="nhaA"
                     /locus_tag="PWY43_001351"
     CDS             10098..11288
                     /gene="nhaA"
                     /locus_tag="PWY43_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDL2136227.1"
                     /translation="MEKLKEFLKSEASGGVLLLVFAFAAIVCANTSLSDFYFEFLQTI
                     VSVQFGEFALNKPLLLWVNDGLMAVFFTLVGLEVKKELFEGSLSSYKKAIFPAIAAVG
                     GMIVPAIIYWAINQNHPEYHNGWAIPMATDIAFAVGIVALLGTRVPLALKAFLLALAI
                     IDDLGAIVVIAIFYSQEISVQALTIAAIAIIGLVLLNRFKVTSLCAYLVVGLVLWTSV
                     LKSGVHATLAGVIIGFCVPLKGKHGERPAEELEHILAPWCAYMILPIFAFANAGVYLL
                     EMGMEQLSSPLPLGIALGLIIGKTLGVFGFSYLAVLFRLASLPQGVNFKQIFAIAVLC
                     GIGFTMSMFLASLAFDVSEAGESITALARLGILLGSAVSAILGYTLLRMATKQNNPEN
                     NATY"
     gene            complement(11365..12789)
                     /gene="panF"
                     /locus_tag="PWY43_001352"
     CDS             complement(11365..12789)
                     /gene="panF"
                     /locus_tag="PWY43_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDL2136228.1"
                     /translation="MNSEMILPLIAYLIFVFGVAFYAYRKRQGGSFISEYYIGGRSMS
                     GFVLAMTTAATYVGASSFIGGPGAAYKYGLGWVLLAMIQVPAVLLSLGALGKKFALLA
                     RKHNSVTINDMLLARYQNKFVVWIAGLALLLSFFAMMTVQFIGAGRLLETTLGIPYQT
                     AVMIFAITVGLYTFIGGFRAVVLTDTIQGLVMIVGTSILLAGVIYAAGGVENAMHTLE
                     AINPQLTTPYGIDERPLDFTFMTSFWVLVCFGLIGLPHIAVRSMACKNSQALHRAMII
                     GTVVIAILMFGMHLSGVLGRAVVPDLKVPDQVIPTLMVQVLPPVVAGIFLAAPMAAIM
                     SSIDSMLIQSSSTLIKDLYLAIKPNAINNEKKIKLFSTITTLTFTVLLVFAALTPPEM
                     LIWLNLLSLGGLEATFLWVIVLGLYWEKANATGAIYSMLAGLSCYVLMTTFKIAIFSF
                     HAIVPSLLIGLIAFLIGNRFAPKV"
     gene            complement(12782..13012)
                     /locus_tag="PWY43_001353"
     CDS             complement(12782..13012)
                     /locus_tag="PWY43_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HDL2136229.1"
                     /translation="MYQQLTTEARWAVLLTLIYLLGWVGFAYFSPQGRGIFGFPIWFE
                     LSCIFLPVLFSLVATIVVKKVYKNIDLEVNNE"
     gene            complement(13060..13377)
                     /gene="metJ"
                     /locus_tag="PWY43_001354"
     CDS             complement(13060..13377)
                     /gene="metJ"
                     /locus_tag="PWY43_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDL2136230.1"
                     /translation="MADWNGEYISPYAEHGKKSEQVKKVTVSIPIKVLEILTNERTRR
                     QIKNLRHATNSELLCEAFLHAFTGQPLPNDEDLMKDRADEIPEAAKQKMCELGIDPDE
                     WQY"
     gene            13532..14287
                     /locus_tag="PWY43_001355"
     CDS             13532..14287
                     /locus_tag="PWY43_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HDL2136231.1"
                     /translation="MKLKSFKPMFMLLSVAFLTACSSSINPETGVRDDPLEGFNRAMW
                     KVNYDYLDPYVLKPVAKGWKEYVPSPVKTGLINVANNLDEPVSFVNRLIEGEGKKAMV
                     HFNRFWINTVFGLGGLIDWASLDEGLRLNNGQRGLGESLGAHGVPSGSYIMMPAYGAT
                     TPRQGIGSAVETGYSVLTYVGSPWSIAKFVVQGVDTRSKLLEQDALLEQAQDPYVTFR
                     EAYFQNLEFKVKDGNVEQSQQEQLSEEELKNID"
BASE COUNT         4257 a         2903 c         3260 g         3907 t
ORIGIN      
        1 ttagttaata aaaaaggagt tgaaatgcgt tcagaactat taaaaacgct tttgttatcg
       61 gggctgattt taccgttatt gatggcattt gccgtaggag cttacggctt tattgtgtgg
      121 atgttacaac tgtttgttat tggtttaccg ggagcgtaga aaatgttagg ttcaatcaaa
      181 gcattattta ctcggcctgc aaccaaaatc gggttgggag tgttagttgg tatcggtttt
      241 attgccggag caattagctg gcatcaattt aataaagcga tggatcatac cagcactgaa
      301 gagttctgtg tgagttgcca ctcaatgcaa cagccgttag aggagcttaa aaaaaccgcc
      361 cactggtcga ataaaagcgg ggtaacggca acctgttcaa gctgccactt accgcacgat
      421 tataccgaaa aatatgcccg caaagtgcag gcaagccgtg aggtgtgggc agaattatcc
      481 ggcaaatata aagaagaagg ctcatttgaa aaacaccgct tagaaatggc agagcgtgag
      541 tgggcaagat ttgccgccaa tggttcaaaa gagtgtaagg cttgccacag ctatgagcga
      601 atgaattttg ataagatgtc ggaagccgct cgtaaggcaa tgaaaccggc agcggagaaa
      661 gatcaaagtt gtatggattg tcacaaaggc attgctcacc acttaccgaa agccgatgaa
      721 aatgcactga aatcgaagtt tgatcagttt gtgaataaat ccccaagcca aggcaaatct
      781 tactacacca aagggaatgc ggaattattc ctagacgaag ggttaagcca aactatcggg
      841 caattagaaa ccgccgtgcc ggtggcattt gtgcaagcag gcaaaaatgc ggatttggtt
      901 gagttgacaa tgtggcgtaa agagaaaggt ttcggtcgca tttggtatca cgatttcggg
      961 aaaaacatta ccgatgcggt tctaaccaaa gagtttatgg caaatcagcc gaaatttgag
     1021 gtgttggaaa ccaaagaaga tccgattacc ggcttgaatt ggcaaaaagt gaaactacaa
     1081 gcgtggattg gcaaagctca attattagac gatgttaccc caatctgggc gaatgcagaa
     1141 aaccaataca aaacccaatg tagcacttgc cacagacagc ctgatgtcgc ccacttcgat
     1201 tccaacagct ggatcggctt attcaacgga atggtcggct ttaccaatat ggataaacag
     1261 accggcaaag aggtgctacg ttatttacaa atgcacgcct ccgatagtga agaagcgaag
     1321 cattaataat attactcccc tctttactaa agaggggaat taatcgagcg ttgaataaca
     1381 ttctctgtac tagctcacgt ttttacaaat aaagaaggac aagatatgca acaatctcgc
     1441 cgtcaatttt taaaaaatat gtctgtgatg gctgccgctg tcacaatgcc gaatttcctc
     1501 gtgcctcgca atgtgtttgc gagtgaagac ctcagtcaat ggaaagtttc cggctctcac
     1561 tggggggcaa tgcgtgccaa aattgagaac ggacgagtgg cagaaatcaa accgttcgag
     1621 tttgataaac acccgaccga aatgctcaac ggcattaaag gcttaattta cagcgattcc
     1681 cgtatccgct acccgatggt gcgtttagat tggctcaaaa accgccataa cagcaacacc
     1741 gcacagcgtg gcgataaccg ctttgtgcgg gtaacgtggg acgaagccct cgacctgttc
     1801 taccaagagt tagaacgggt acaaaaagag tacggcccgt gggcgttaca caccgccaat
     1861 gtgggctggc gttcaaccgg tcaattccac agttgcggta accatatgat ccgagcgatt
     1921 gcgatgcacg gtaacagtgt cggcacggca ggcgactatt ctaccggtgc agggcagacc
     1981 attctgccgt atgtgctggg ttcaaccgaa gtctattcgc agggcacgtc ttgggaaatc
     2041 atcttaaaag agagcgaaaa catcattttc tgggcaagcg acccggtgaa gaacctgcaa
     2101 gtgggttgga actgcgaaac ccacgaagcc tatgcctact tggaaaaact gaaagagaaa
     2161 gtggcagcaa aaggcgtgaa tgtgatctgt gttgatccgg tgaaaagcaa aacccagaat
     2221 ttcttaggtt gcgaacagca gtacatcaat ccacaaaccg atgtgccgtt tatgttggca
     2281 cttgcccaca cgctctacac cgagaacctg tacgacaaaa aattcttgga tatgtacacg
     2341 gtcggctttg aaaaattcct gccgtatctg ttaggcgaaa ccgaagacaa agtggcgaaa
     2401 accccggaat gggcggctga aatttgtggt atttccgccg acaaaatccg tgaatttgcc
     2461 cgaatgcttg ccggcaaacg cacccaattt attttcggct gggcaattca acgccaacaa
     2521 cacggcgaac aaccgtactg gatgggagcg gtgctagcct caatgttagg gcaaatcggc
     2581 ttggaaggcg gcggcatcag ctatgctcac cattacagct ctatcggtat tcctgaatcc
     2641 ggtgcggcaa tgccgggggc gttcccgctc aatattgatg aggggcaaac cccgaaatac
     2701 gacaacaaag attacaaagg ctacagcgac accattccgg tggctcgtat gaccgacagc
     2761 ttgctccacg ccggcgaaac cattgattac aacggcaaaa aagtgactta cgctccgttc
     2821 aaaatggcga ttttcaccgg ctgtaaccaa tggcaccgcc attccgagcg gaacaaaatg
     2881 aaacaggcgt tccaaaaact ggaaaccatt gtttcgatca actacagttg gacggcaacc
     2941 tgccgcttct ccgacatcgt gctgccggcc tgtaccccat ttgaacgcaa cgatattgac
     3001 gcctacggct catacagcaa ccgtggcgtg attgcaatgc acaagctggt tgatccactc
     3061 tacgactctc gctccgactt tgagattttc cgagatctct gccgccgttt cggtaaagag
     3121 aaagagtaca gccgtggaat ggacgaaatg cagtgggtag aaaaactcta caaagattgt
     3181 cgccgtgaaa acagaggtaa atttgaaatg ccggagtttg ctgaattttg gcaaaaaggc
     3241 tatgtgctgt tcccagaggg caaaccttgg gttcgccacg ccgatttccg tgaagatccg
     3301 gaattgcacg cacttggcac accgtccggc tttatcgaaa tttacagcaa caaaatcgcc
     3361 tctttcggct atgacgattg caaaggtcac ccaatgtggt ttgagaaagc cgaacgttca
     3421 cacggcggta aaaattccga caaattcccg tactggttgc aatcagttca cccggacaaa
     3481 cgcttacact ctcaactttg tgaatcgaaa gaactgcgtg aaacttacag cgtgcaaggg
     3541 cgtgaaccgc tttacattaa tccacaagat gccgccaaac acggcattca agacggagat
     3601 ttagtgcgag tgtttaacga ccgtgggcaa gcgattgtgg gggcggtgct ttccgacaac
     3661 ttcccgaccg gcgtagtgcg tttacaagaa ggggcttggt attcacccct tgacgaaagc
     3721 gtgggggcga ttgacaccta tggcgacccg aacacaatga ccctcgacat cggcacgtcc
     3781 aagttggcac aagcggtttc cgccaacact tgcttggtga acatcgagaa atttatcggc
     3841 aatgccccgg ctccgaatgg ctttaacggt gcgattgagg taacggctta atgacgttgg
     3901 caatcagtcg tgaagaacgg caatttatct atggctggtt taacgcaatg ctcgcccgag
     3961 aactttcgga cgagcagtta aatgccttac aagcgggcga atttgacgat ttttttgcat
     4021 acctcagcga attgggtttt gccgaacagg ttgcaaaatt tcaagcagaa gtgaccgctt
     4081 gtaggcagct ggaatttcct cgtctagaac tggcggctga ctttgcccaa ctgtttttat
     4141 tggaaggcaa tctcagtgcc attccttacg cctccgccta tttaagcggc gaagcgttag
     4201 cgaaaaattt ggcggaaatg gacagactgc tgggcgaatt tgggctgcaa gttaatcgag
     4261 aaaccaaaga gccgagcgat cacctttgcg tttatctcac cctttttgac cgcttgcttg
     4321 ctgaaaaacc gttggaaacg caacagcaat ttctgcaaaa ccaactgatg agctggctcc
     4381 ctgcgtggtt cgccaaagcc aaaaccgtca aaactaacac cggcgtttat ccggcgttgc
     4441 taaggttgtt tgaacaggtg ttgctgaagg aacaaagata aagttattta tttgagaatg
     4501 atttgtattg agcgaattga gaaaggaagt taaaatttca acttcctttt ttattggctg
     4561 gatttatcca cattattaca agcggttatt tttataaaaa aatttacaaa ggagacatta
     4621 atgcaaaata ataaaaccac ctacctaaat tggtacgatt tatgcgtgct aaccctgatc
     4681 ctgtttggtg aggcaattta tacctcaacc cagcaatatt tcatcaccac cgaaatgcaa
     4741 ggtaattttg aagaaaattt aaccttttca aatgaacaaa attattgggc attactgacg
     4801 caacttgcat tgttgctgat tgcaatgggc tatctaaaat ggcggaattt tgatttcagc
     4861 cgttgggtga ttcgtccctc ttggctggct ttttttagcg gtatcgggtt gtttatcttg
     4921 ctctcgttag cgatggatct ctattttgaa acattatatg gtgtttacaa cctagtgtat
     4981 ccggagccat tttgggcagt attgagagaa gtgaatattt ctaccgtgct gtatgcgatt
     5041 ttaaacggct tttatgaaga aatctttttc ctcggtattt gcttggcggt taaacctgaa
     5101 tatttaaaat gggcggtgat ttattcactg attattcggg tgagttttca tacctatcaa
     5161 ggaatggaaa gtgcgttagg attaggaatt tggctcggtt taattattta tttgctctac
     5221 tcacgctcaa agcagaaaaa cctatttcca ttttgggtgg cacacagtat tgcggatatt
     5281 ttggggctga gtttgttggc ttatttttag ttaaaggtgc tacatttaag cggcataagt
     5341 tgatagagct gatcgtacca gtagcataaa aacattatta tttgtatgga aaggtgcttt
     5401 taggttacaa tataaccgat agttattggt tataactaaa aattattttt aatttaacgg
     5461 agcaaatatg aagagtatca aaaaattaac cctagccact ttgattttag tgggtttaac
     5521 tgcttgtggt agcggtggtt caggtagcag ttctaataat gctaaatcga aaccacaagt
     5581 tgagttaaaa aaaactgaag aggataaaaa ggctgaagaa gcaaaaaaaa ttgcagaggc
     5641 aaaaggaatt ggcaataaag aatataaaga tggattaaat gagttagaag aaaaaaaatg
     5701 aaacaggtaa aaacggagaa gagattcgaa cctatggtta tctctataac agccattatt
     5761 ccgttgtaac tgccaaaatg aaacaaactt tacaggaaaa tggtcgtcaa atggaaccta
     5821 cggttgaagt gaaagggctt aaaactgaga atttaccgac agagggcaaa gcaacatata
     5881 aaggagaagc ctttgattct cacggtaata acagcaattc agtagtaggt ggagaattaa
     5941 tctataatgt tgatttctct agtcgtaccg gatcaggtct agttaaaaat gtacagggcg
     6001 gttctatcga gttagcacaa ggcgaaataa aaaatgatag tattatagct tctgctcacc
     6061 aaaagtataa tgatcaggct gttggaaatg gtagttacaa tatccaattc tttggtccaa
     6121 atgtagaaga aattggaggg aaaattgagc ttaatggtga gggcgaaggc tcaatgaagc
     6181 agatgttagg tattgcagga acccgagaag aacaaaaata acatttaata cccaatgccg
     6241 tttaatctta aacggcattc ttttttacta tgtataaaaa acattttcta tttttacttg
     6301 tttcgccttt agtaatggcg gaaacggttg agtttcagca agatcccaaa gagtttgatg
     6361 ggcagcgaca agcggttaaa tttgagcaag aaattgcaaa aaaggattta aaattaaccg
     6421 cttacttgga ggaagataat tcccttaaag gtattgaaca gcacattaac caagcgattc
     6481 ttagacaaga ttgggcgaag ctggaaaggc tgcttggcga ttatgcaaag caagagcaat
     6541 ttgatccgct acttttagac tatgctttgg cggcattggc ttatgctaaa aaagattatc
     6601 gctctgcggc gttttattat cagcagttgc tgaaaaaaca gcctgattta acctaccctc
     6661 gttttgattt ggctctgatt ttaaaagagg attatcgcta cagggaagca gagcaagaat
     6721 ttaagcaact caaaaccgat gatttacgca atgcggtgaa cagtgcgttg gctcaaatag
     6781 aggaggaaga gagctggaag ccggattttt atttgcaata tactcaaacc aacaatgtga
     6841 ataatgcttc ttcgtcgcca acggtaaatg taaacgggcg gatttttatt aaagaatcag
     6901 acagcttgcc gcagtcggca aaaggcgtaa gatatggcgt tggcttgagt aaaatgcgaa
     6961 atatcggcgg taatcatttt gtgggagtcg atcttggcta taacggcgtt tattactgga
     7021 ataaccacga ttatagcgaa cagtcggtct cactaacacc aaaatatagc tacagaacgg
     7081 caacttatcg tatcggggtt tcgcccttta ttgagcagaa ttggctgggt agctcacggt
     7141 ataactatca atttggtagc agtatcaatg ctgttaaact ttttgaacga gggctgattt
     7201 ccggctcatt cagccatctg caaaaacgtt atagtgaaca gcatattgcc gaccgttaca
     7261 atggttatcg caaccaactc acgctaggta cgcaatggca agcggtcaaa aattggcgat
     7321 tttttgcaaa tcttaacgct agccgtgaaa ttacccgtga gaaagctcaa tcttccaata
     7381 agtggctggc gaccattggc gtaatttacc aaaccccaag ctgggcaagt tcggtaagtg
     7441 tcggatatgg caagcgttat tttgctgata aacattatct gtttggctat aaacgccaag
     7501 ataaggaata tcaagcgaat gtggcggtgt ggaatcgaag ttggcactgg catgggattg
     7561 tgccgaaatt aaatttccgc tatcaaaaaa tcgacagcaa tattgccgat ttctactccc
     7621 gtagcaataa agaagtcttt atgacgttag agaaactatt ttaaggctgt gtatgaatgt
     7681 cggggagaaa aattttcgcc cccatcaata ttaacttaat tgaatttttg gtaatttaac
     7741 tgcaaccatt gtagcccaat taccgcaatt acattatcaa ttgtgccgtc ttccacccat
     7801 tgatatgcct gttcacggct aaccacatgc actaaaatat cttcgttttc ttcttccaat
     7861 aagccgtgaa cctcgccgga tttcactttg gtgttgtcaa tcaagccgag aaacaggtgc
     7921 aggcgttcca gttgtccacc ggggctgtcc catacgctta aggcgtgttg cacttgtgtt
     7981 acttccaagc cggcttcttc tttggcttca cgcattgcaa cttctgccgg ctcttcgttg
     8041 cctttatcta ccataccggc aaccaattcc agcaaccaag gcgatttctt ctgccctgca
     8101 tcgtaagcac cgatacgcac ttgctcaatt agcactacct tgtctaagat aggatcgtaa
     8161 gcaattaaag ctgccgcttc gcctttaatt aagagttcac gcaccacttc accgctcatt
     8221 tcaccggaaa acagtttgtg gcggaaagtg attttttgta gctcaaaatg ccctttatag
     8281 acggtttctc ggtttagaat ctgtaaatca ttgtgctgat attgggttag cttcatatta
     8341 gttcctagct tttatgtaaa cgatcggtgt gtacaatata taatgctata atcatcacca
     8401 aaatagagag tgcgaataat agtgtatgca acgcattaga atgatcgaca ataatcaatc
     8461 ttaccatggc agtaatcccg atatagagga aatagcgtaa cggaaagtga taattcgatt
     8521 tgaaatattt cacaatcaac gcaagaaact caaaatagag gaagaaaatc acaatttttt
     8581 ccacaatttg gaatttttca ctgtcgctaa aagagagttc atagagtgaa taggcttcag
     8641 caaacagcga gtaagaaagc aatgctcctg ttactagcaa ggcaatattc ataatccact
     8701 gaaaggtttc ggaagcaatt ttgccaaaac tggaatggtg ttcttctaaa gattttttag
     8761 gcattcgtta tccttaatta acatcataaa caagtgattg gcactttata ggctgaaact
     8821 gcccgttttg ccaatggtat agcaaaggtt cagataatag catattttga ggttgttgat
     8881 agaagtagct taaatagaac ggcagttcgc tcacaaaatg cagcttgcct ttcggacaat
     8941 gggcggaaaa gatctgtttt tctaacggta aatgttttac ttcggacagt ttcacaatcc
     9001 ccaccccttg cgatttcaat atcgcctctc gcaagcacca gctcaaataa aagcgttcgg
     9061 cgaggttgct tagaatgggg gagtggttgg ctaataaaac attttgttct tcagcgtttc
     9121 cgtaatggca gattaaatcg tggtaacgtc tgattttttg tgggtgttca atatcaatgc
     9181 cgaccgctaa ttttgcaaaa gaatacgaaa aaatgaccgc tatccattcc cctgaatggc
     9241 tgatgttaaa gtcaatttgc tcagaatcga caaaagtgcg accgcttggg gagcgttgca
     9301 tatcagctaa taaatctaaa tccagatggt gttttttaaa caattccgtc agcagaaaaa
     9361 aggcgactcg gcggctttgc catttttgca cctgtttttc agttaaaccg ccgggcattt
     9421 cagggtagga gaaatcactc ggcagaggtg ctttccgatg aataaacgca atttcaatat
     9481 ttgcaaaatt ttgcataaaa atcaccgctt gaaggcaagt aacagtacgc caccagcaac
     9541 taagccaatg ccaagccaat cttgtccgct tgggcgttcg cctaagaaaa ttactgcaaa
     9601 aagggtaact aacaccaagc tgaatttatc gattggagcg acttgggaag cattacccat
     9661 ttgcaatgct ttgaaatacg ccgaccaaga caatcctgtt gccaagccgg ataagattaa
     9721 aaacagccag tttttgctgc tcaagctatc taacggctgc cattttccgc tgtaagtgag
     9781 aaaggcaata agtgcaataa taataactac cgtgcgaata aaagtggcga aatcagaatc
     9841 tacgccttgt aagccaactt tggcaaaaat ggcggttaat gctgcaaaaa gtgcagaggc
     9901 taatgcccaa tagagccaat cgtgtgtcat aacaaaattt taatatttta ggttaatttc
     9961 taatcatatg gagcaataaa atgcgagtta gttctagttt atcaaatttt ctatttgatc
    10021 ttcgcaaaat ttttggtaaa agatggaatg tatagaaatg aaaatacaat aaacaaatat
    10081 taataggaga aagttaagtg gaaaaattaa aagaattttt aaaatctgaa gcctcaggag
    10141 gcgtattgct tctggttttt gcttttgcag caattgtgtg cgccaacacc tccttaagtg
    10201 atttctattt tgagttttta caaacgattg ttagtgtaca gtttggtgaa tttgccttga
    10261 ataaaccgct attactctgg gttaacgatg gcttaatggc agtgtttttt acgttggtgg
    10321 ggctggaagt aaaaaaagag ttgtttgaag gttcgctttc aagttataag aaagccattt
    10381 ttcctgcgat agcagcagta ggtggtatga ttgttccagc gattatttat tgggcgatta
    10441 atcaaaacca ccctgaatat cataatggtt gggcaattcc aatggcaact gatattgcct
    10501 ttgcggtggg cattgtggcg ttattaggca ctcgtgtgcc attggcttta aaggcatttt
    10561 tgttggcatt agcgattatt gatgatctag gagcgattgt ggtaatcgcc attttctatt
    10621 cgcaagaaat cagtgtgcaa gccttaacta ttgccgcgat tgcgattatc ggcttagtgc
    10681 tacttaaccg ctttaaagtg acctcactct gtgcttattt ggtagtaggc ttagtgctat
    10741 ggacttccgt tttaaaatca ggggttcacg ctacccttgc cggcgtgatc atcggcttct
    10801 gcgtaccgct gaaaggtaaa catggggaac gaccggcaga ggagctagaa catattcttg
    10861 ccccttggtg tgcttatatg attttaccga tcttcgcctt cgctaatgcc ggcgtttact
    10921 tattagaaat gggaatggag caactctcat caccattgcc gttaggtatt gcgctcggct
    10981 taattatcgg caaaacctta ggtgtattcg gttttagtta tttagcagta ttattccgcc
    11041 ttgccagcct gccgcaaggg gttaatttta agcaaatttt tgccattgcg gtgctatgcg
    11101 gtatcggttt tacaatgtcg atgttcttag caagtcttgc ctttgatgta agtgaggctg
    11161 gggaaagtat taccgctcta gctcgattag gtattttact cggctctgcg gtttcagcga
    11221 ttttaggtta tacgttactt agaatggcga ctaagcagaa taatccagaa aataatgcca
    11281 cttattaact acaagcggtt gaattgagcg gaaattttgc aaaataggca tagtgaactt
    11341 ccataccact atgcctattt tattttacac cttaggagca aatcggttgc cgattaagaa
    11401 agcaatcaaa ccaattagca gtgaaggcac aatggcgtgg aagctaaaaa tagcaatctt
    11461 aaaggtagtc attaatacat aacaacttaa gcctgccagc attgagtaaa tagcaccggt
    11521 ggcatttgcc ttttcccaat acaaccctag tacaattacc cataggaaag tcgcttctaa
    11581 accacctaaa gagagtaggt tcagccaaat taacatctca ggcggggtta gggcggcaaa
    11641 cacgagtaat accgtaaaag taagagtagt aatagtggag aacaatttga tttttttctc
    11701 gttattaatg gcattcggct taattgccag atagagatct ttaattaagg ttgaagacga
    11761 ctgaatcaac attgagtcaa tcgaagacat aatcgctgcc attggggcgg caaggaaaat
    11821 accggcaacc accggtggta gcacttgtac cattagtgtt ggaatgactt gatccggcac
    11881 tttgagatca ggcacaaccg ctctgcctaa tacgcctgaa aggtgcattc caaacattag
    11941 aattgcaatg actaccgtgc cgataatcat tgctcggtgc aaggcttggc tgtttttgca
    12001 tgccatactt ctgaccgcaa tatgcggtaa gccgattaaa ccaaagcaaa ccaataccca
    12061 aaatgaagtc ataaaggtga aatcaagcgg gcgttcgtca atgccgtaag gggtggtaag
    12121 ttgtgggtta atggcttcaa gggtgtgcat tgcattttcc actccgcctg cggcataaat
    12181 cacaccagcg agtaaaatgc ttgtgccgac tatcattact aaaccttgaa tagtatcggt
    12241 tagcaccacc gctctaaaac cgccgataaa ggtataaagc ccgactgtga tggcaaaaat
    12301 cattaccgct gtttggtaag ggattccgag tgtggtttca agtaagcgac cggcaccgat
    12361 aaattgcacc gtcatcatcg caaaaaacga aagtaataag gctagtcctg caatccaaac
    12421 cacaaatttg ttttggtaac gggctaataa catatcgtta atggtgacac tattatgctt
    12481 gcgggcaagt aaggcaaatt ttttgcctaa tgctccaaga gagagtagca ctgcgggcac
    12541 ttgaatcatt gccagtaaca cccagcctaa accgtattta taggcagcac ccggcccacc
    12601 gataaaggaa cttgccccca cataagtggc ggcggttgtc attgctaaca caaagcccga
    12661 cattgaacgc ccaccgatat agtattccga aataaagctt ccgccttggc gtttacggta
    12721 agcataaaac gctacgccaa acacaaaaat taaataagca atcaggggta aaatcatttc
    12781 actattcatt attcacctca agatcgatat ttttatacac ttttttgacg acaatggtgg
    12841 caactaagct aaatagtacg ggtaagaaaa tgcaagaaag ttcaaaccaa atcggaaagc
    12901 cgaaaatgcc tctgccttgc ggtgaaaagt aagcaaaacc tacccagccg agcagataaa
    12961 tcagcgtaag caacacagcc caacgtgctt ctgttgttaa ttgttgatac ataaagtctc
    13021 cttaaaacat aaaaagaacc tcgcagttgc aaggtgtgtc taatattgcc attcgtccgg
    13081 gtctattccc agctcacaca ttttttgttt tgccgcttca gggatttcat ctgccctgtc
    13141 tttcatcaag tcttcatcat tcggcaaagg ctgaccggta aaggcgtgca aaaaagcctc
    13201 gcacaataat tcgctgttgg ttgcgtgacg aagatttttg atttggcgac gggttcgctc
    13261 attagttaaa atttccaata ctttaattgg aatggatacc gtcacttttt tgacttgttc
    13321 actttttttg ccgtgttcgg cataagggct aatatattcg ccgttccagt ctgccatttg
    13381 tttgctcctt aaatgttgcc tgtattgtaa tcaaaagcga actatatctc aatctagacg
    13441 tctagatttc tagaggcaaa acggcttttt gttttgtatt ttttatcaac ttcgctacaa
    13501 tggacaaatt tctctgcaaa aggttcaatt tatgaaactc aagtcgttca aaccgatgtt
    13561 tatgctactc agtgtggcat ttttaaccgc ttgttcatca agcattaacc cagaaaccgg
    13621 cgtgcgagac gacccacttg aggggtttaa ccgtgcgatg tggaaagtca attacgacta
    13681 tttagaccct tatgtgttaa aaccggtagc taaaggttgg aaagagtatg taccaagccc
    13741 ggttaaaaca ggcttaatta atgttgctaa taaccttgat gagccggtga gctttgtaaa
    13801 ccgcttaatt gaaggagaag gtaaaaaagc gatggttcac ttcaaccgct tctggatcaa
    13861 cactgtgttt ggtttaggtg ggttaattga ctgggcgagt ttagatgagg ggttacgttt
    13921 aaataacgga caacgtggtt taggtgagag tttaggcgct cacggcgtgc cttccggctc
    13981 ctatattatg atgccggctt atggagcaac cacacctcgt caaggtattg gtagtgcggt
    14041 ggaaaccggt tattcggtgc tgacttatgt tgggtcacct tggtctatcg ctaaatttgt
    14101 ggtacaaggg gtagataccc gttctaaatt attagaacaa gatgcgttgt tagagcaagc
    14161 tcaagaccct tatgttacct tccgtgaagc ttatttccaa aaccttgaat ttaaagtgaa
    14221 agatggcaat gttgagcaaa gccaacaaga gcagctttcg gaagaagagt tgaagaatat
    14281 tgattaattg atgtgaacga gggatggtac gcctcatcta aagcgca
//
LOCUS       DANKBV010000056        13809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.056, whole genome shotgun sequence.
ACCESSION   DANKBV010000056 DANKBV010000000
VERSION     DANKBV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.056"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            111..1283
                     /gene="cytX"
                     /locus_tag="PWY43_001356"
     CDS             111..1283
                     /gene="cytX"
                     /locus_tag="PWY43_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HDL2136232.1"
                     /translation="MNRTLSTLNSGLIWAGAGISASAIVVGTWIAPLGWQQGLLAIVL
                     GHLLGGILFFAAGLIGAKTGKNAMQTVQISFGRGSVFFSLANVLQLVGWTAIMIYTGA
                     EISNALSLALWEYSAFSLWAIVLGLLIVLWLFTGSNQVGKFKLVTLIAMFLLTLWLSI
                     KVLNGQENFPVSGEMPFSTAVELATIMPLSWLPLVSDYTAKAKKPFAATLSATIGYLI
                     TSAWMYALGLGMVLFTGQTEIAPMLLMTGMSLIGIMVMILSTVTTTFLDAYSAGVSAG
                     NITAKFKETPTAILVTIIGTLLAISLPVTQYENFLLLIGSVFAPMIAVQIADFFVLKR
                     HQAQPNVDWVAAGLWLVGFVLYHLLSYHQVHLLLGLTIPVMLVIFLSTIFVRRFTK"
     gene            1322..1531
                     /locus_tag="PWY43_001357"
     CDS             1322..1531
                     /locus_tag="PWY43_001357"
                     /inference="COORDINATES: protein motif:HMM:NF015737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL2136233.1"
                     /translation="MESLVLNNDWVVTPQAQLAYHTINGKADEERLNLLVARAGVRVA
                     KGFALNNGWNLQPYAEVNGIAEKGQ"
     gene            1539..1715
                     /locus_tag="PWY43_001358"
     CDS             1539..1715
                     /locus_tag="PWY43_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL2136234.1"
                     /translation="MQVNQYQFGVAENKGRVQGSLGLSAGNGNHRLGLEASVASGKKL
                     KQPFNVLVNYRYQW"
     gene            complement(1756..3612)
                     /gene="sppA"
                     /locus_tag="PWY43_001359"
     CDS             complement(1756..3612)
                     /gene="sppA"
                     /locus_tag="PWY43_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2136235.1"
                     /translation="MLTILKAFYRIFRCIRECVINLFFILFILLLVPVVAFISSSGST
                     QKPVFNQGALRLNLDGYLADNREEFTDFYRLVQSELGSSEPFKISTFDVVQAISHAKN
                     DPKITGLVLDLQKLQSADFSSLDFIGNEINNFKQSGKPVIAVGEDYSQKQYYLASFAD
                     EIYLNKVGAVDLKGLNYSNTYFKTLLDKIEAEPHIFRVGTYKSAVEPFMRDDMSEEAK
                     QNARGWLNGTWQQTVATLAQNRNIEPQALDLSPANYIEKYKQAKGDDAQLALNQKWVS
                     QLVSSQESRDKLIAQFGKNSEGSYNQIEFLDYMTELNDRFAEVNAPKIAVVNVEGAIA
                     GGESDEMNVGSETLVKLLQQARDDKNVEGLILRINSPGGSAVASELIRQEVEAFQQAG
                     KPVVASMGGMAASGGYWIAATSDKIIASPNTLTGSIGIFGLAVTFEKTAKNLGVSEDG
                     IATSALAQQAGLKSLPQEQAEVLQIGIENGYDRFLELVSRGRKMPKEVVDKVAQGQVW
                     LGTDALKHGLVDELGNFNVAYNALSELINQKREAENQPKVEQFGLQWFAEQDDSLFGT
                     LARDFKAQLQFSVANWLDLPLLKQPQQQLETLSRFNDPKQMYLYCLNCGKID"
     gene            3720..4283
                     /locus_tag="PWY43_001360"
     CDS             3720..4283
                     /locus_tag="PWY43_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2136236.1"
                     /translation="MQILDLLHHRRSNKKFGDKAPNAEQLEQILKAGLRVPDHGRLKP
                     YRFVVIEKSGMPQFGECLKSAVAEFDMGEERLKKAEKLANQAPMIIGVVATLDDSIAK
                     VPSWEQMITAGCATYAIQLAANAQGFDTVWITNKWINGSALRSAFGCQESDKIIALIM
                     LGSPAEGEQIALAPESESLEGFVRYLK"
     gene            complement(4354..6678)
                     /locus_tag="PWY43_001361"
     CDS             complement(4354..6678)
                     /locus_tag="PWY43_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL2136237.1"
                     /translation="MTDLNLKISQIIAGELNVGSHQILAAITLLGEGNTIPFIARYRK
                     EVTGGLDDTQLRHFETRLIYLREMDERRQTILKSIEEQGKLTDELKAKILSSESKTEL
                     EDLYLPYKPKRRTRGQIAIEAGLEPLADSLWNDPKQDPETLAANFVDAEKGVADTKAA
                     LDGARYILMERFSEDAELLAKLRQYLTAYATLESKVIDGKEEEGEKFRDYFAHSEPFN
                     SVPSHRALAMFRGRNEGVLSLSLNADPTAEEGTKSSHCEEIIREHLGVIFNNQPADKW
                     REQVIAWTWKIKAALHLETELMATLREKAEEEAIDVFARNLSALLMAAPAGARNTMGL
                     DPGLRTGVKVAVVDNTGKLLDTATIYPHTGREAEAQLAIFSLIKQHNVELIAIGNGTA
                     SRETERFAKAVIKEIKENKPQTVVVSEAGASVYSASEFAANEFPNLDVSLRGAVSIAR
                     RLQDPLAELVKIEPKAIGVGQYQHDINQSQLARKLDAVVEDCVNAVGVDLNTASAPLL
                     ARVAGMTKTLAQNIVAFRDENGRFTSRSELKKVPRLGPKAFEQCAGFMRIIGGKNALD
                     ASSVHPEAYPVVEKILQATTSTLAELMGNNSKIHSLNAKDFVDEQFGLPTVNDIFKEL
                     EKPGRDPRGEFKTAVFMDGVEEITDLKAGMILEGTVTNVANFGAFVDIGVHQDGLVHI
                     SMLSNSFVEDPHTVVKAGDVVKVKVLEVDAARKRIALTMRLDDKPTDKKEPTSGRNSA
                     EIRKNTQKNDRLQSNNRNAFGNNAFADALKGWKK"
     gene            complement(6678..7232)
                     /gene="pilW"
                     /locus_tag="PWY43_001362"
     CDS             complement(6678..7232)
                     /gene="pilW"
                     /locus_tag="PWY43_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HDL2136238.1"
                     /translation="MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARIN
                     LALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLAYYYQQTGDAENAEKAYQTALKL
                     SQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
                     QKIAETLTELEKLDKPRANLLKEN"
     gene            7452..7703
                     /locus_tag="PWY43_001363"
     CDS             7452..7703
                     /locus_tag="PWY43_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2136239.1"
                     /translation="MKIVVFQRKALKQLRKIPTGAEIRRKCDDLAYFPYCANIKALTN
                     HQYDYRFRVGNYRIFFNIEGETMNIVSIEEVKKRDERTY"
     gene            7687..8034
                     /locus_tag="PWY43_001364"
     CDS             7687..8034
                     /locus_tag="PWY43_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2136240.1"
                     /translation="MKELTNIQYLNNEEGKPAFAVLPIATLEWLKQKANIKTAIETGI
                     PSAVAGIALDNNYSALRAWREYLGLTQEEVAQRLGISQSAYSQHENAKNLRKTTRIKI
                     AYALGLNEEQLDF"
     gene            complement(8075..9235)
                     /gene="trmG/rlmN"
                     /locus_tag="PWY43_001365"
     CDS             complement(8075..9235)
                     /gene="trmG/rlmN"
                     /locus_tag="PWY43_001365"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDL2136241.1"
                     /translation="MSELITNAGCGSAEQPKEKINLLNLNRQEMRELFAEMGEKPFRA
                     DQLMKWIYHFGEENFDNMSNINKVLREKLKRIAEIKAPEVAVEQRSADGTIKWAMQVG
                     DQQIETVYIPEDDRATLCVSSQVGCALACTFCSTAQQGFNRNLSVAEIIGQVWRASKI
                     IGNFGVTGVRPITNVVMMGMGEPLLNMNNVIPAMEIMLDDFAYGLSKRRVTLSTSGVV
                     PALDKMREQIDVALAISLHAPNDELRDELVPINKKYNIKMLMDSVNKYLEVSNANHGK
                     VTIEYVMLDHVNDSTDHAHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQK
                     TLMEYGFTVTVRKTRGDDIDAACGQLAGDVIDRTKRTLEKRKFGQGIAVKTH"
     gene            9495..10391
                     /locus_tag="PWY43_001366"
     CDS             9495..10391
                     /locus_tag="PWY43_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2136242.1"
                     /translation="MKLSTIKKALVLTFGLSAAMAMAEPFKVVTTFTVIQDIAQNVAG
                     DKATVESITKPGAEIHDYQPTPKDIVKAQKADLVLWNGMNLERWFERFFENVKGKPAV
                     VVTEGITPMPITEGEYKNLPNPHAWMSTDNALIYIENIRAALVKYDPQNADTYNANAK
                     AYAEKVKGIAEPLRQRLAFIPEQQRWLVTSEGAFSYLAKDYNLKELYLWAINAEEQGS
                     PKQVKKVIDGVKANKIPVVFSESTVSDKPAKQVAKETGALYGGVIYVDSLSEKDGPVP
                     TYLDLLKVTIGTIVEGFERSKK"
     gene            10404..11147
                     /locus_tag="PWY43_001367"
     CDS             10404..11147
                     /locus_tag="PWY43_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2136243.1"
                     /translation="MTTVSASISVENLSVRYNNGHIALQDVSFHLENGTICALIGVNG
                     GGKSTLFKSLMGLVKPLTGSVKLSQLPIAKALKQNLVSYVPQSEEVDWQFPVSVYDVV
                     MMGRYGYMNFLRRPSQTDKLKVEQAMQRLDVLHLKDRQIGELSGGQKKRVFLARALAQ
                     ESKIILLDEPFTGVDVKTENAIVELLKQLKNEGHLILVSTHNLASVPSFCDQVLMVNR
                     TLLAKGKTEEIFNNQNLERVFGGLLHYQK"
     gene            complement(11204..12013)
                     /locus_tag="PWY43_001368"
     CDS             complement(11204..12013)
                     /locus_tag="PWY43_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2136244.1"
                     /translation="MLNWILEPLQFEFMQIALITAILVAGVSAVLSCYLVLKGWSLMG
                     DAISHAVLPGVVISSLAGLPLSIGAFFSGLGCALSVGYLKENSRLKEDTVMGIVFSGM
                     FAFGILLFTAFNTGEHLTHILFGNLLGVTKSDLLQTLLISLFTFVVMVAKRKDFLLYC
                     FDVNQAKVAGLPVRLLHYGLLSLLALTIISAMQVVGVILVVAMLITPGITAYLLTKRF
                     DSMLLIALGVAIFSSVFGTLLSYHIDAATGPTIILVQAAIFFLSFAYSKIH"
     gene            complement(12006..12866)
                     /locus_tag="PWY43_001369"
     CDS             complement(12006..12866)
                     /locus_tag="PWY43_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2136245.1"
                     /translation="MLDLLLEPFTYNYMQKAIFVSMGVGLVCAFLSAFLMLKGWSLIG
                     DALSHAVVPGVAIAYALKLPYAFGAFFSGILAALSILWVKSLTKIKEDAVIGFIFTTF
                     FALGMFIVSLNPTSVDVNSIVMGNILGIADDDLWQVVLIIAISLLGLILFWKDLLLVF
                     FDEHHALSVGLSPLRYKVIFFTLLSACVVVALQTVGAILVIAMVVTPGATAYLLTDRF
                     PRLVLIAMLIGTLTSGIGAYLSYFLDGATGAVIVCLQTLIFLFAFCFAPKYGLITQKR
                     KLHRVEVQNA"
     gene            complement(12940..13725)
                     /locus_tag="PWY43_001370"
     CDS             complement(12940..13725)
                     /locus_tag="PWY43_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDL2136246.1"
                     /translation="MMKKITILGLGWLGLPLAEALQNKGWRVAGTKRTVGELAIECYP
                     LDLNALAVTSEIEQLLAADALVIALPLSQIRPENYGCGIQTLVSLAIEKGLKHLVFTS
                     STSVLPMKSGVFDENAVVDSANLLVKMENWLLSQPIHCDILRLAGLVGKKRHPVFYLA
                     GKQNLSGAGQPVNLVHLEDCIAAISLLLEQPNGQRIFHLCAEQHPSRKDYYSEMAKRF
                     GLADLHFSEENQPLVRLIKADKICQELGFRYYYPDPYQFKVDI"
BASE COUNT         4031 a         3065 c         2845 g         3868 t
ORIGIN      
        1 tgtaatgcat accggtgtcg gcagtgccgc ggtggtaagt gccgttaccc aagcaaaaga
       61 ttggcaacaa gcggtcaaaa acttagcaaa atttgcaaat ggaggccgta atgaatagaa
      121 ctcttagtac gctaaacagt ggtttgattt gggcgggagc gggcattagt gccagtgcaa
      181 tcgtggttgg cacttggatt gccccacttg gctggcaaca aggcttgctg gcgatagtgc
      241 tagggcattt attaggcggc attttgtttt ttgctgccgg cttaattggg gcgaaaaccg
      301 gcaaaaatgc gatgcaaacg gtgcaaattt cctttggcag aggctcggtg tttttctccc
      361 ttgccaatgt gctacagctt gttggttgga cagccattat gatctataca ggggcagaga
      421 tcagtaatgc ccttagcctt gccttatggg aatattcggc tttctcgctt tgggcaatcg
      481 tgcttgggtt gctgattgtg ctatggttat ttaccggctc taatcaggtc ggaaaattca
      541 agttagtgac attgatagcg atgtttttgc tcaccttatg gctaagcatt aaggtattga
      601 atgggcagga aaattttcct gtttcgggtg aaatgccgtt tagcaccgca gtggaattag
      661 caactattat gcctctttcg tggctacctt tggtatcaga ctataccgct aaagcgaaaa
      721 agccttttgc cgcgaccctt tcggcgacca taggctattt gatcaccagt gcgtggatgt
      781 atgcgttagg tctgggtatg gtgctattta ccgggcaaac cgaaattgcc cctatgttac
      841 taatgacagg aatgagcctt atcggcatta tggtgatgat tctttcgact gtgactacca
      901 cgtttcttga tgcctattcc gccggtgtaa gtgccggcaa tatcacggca aaattcaaag
      961 aaaccccaac cgccattcta gtcacaatca tcggcacgct actggcaatc agtttaccgg
     1021 ttacccaata tgaaaatttc ctgctgctga taggctcggt attcgccccg atgattgccg
     1081 tgcaaattgc ggatttcttt gtgttaaaac gccaccaagc acagcctaat gttgattggg
     1141 ttgctgccgg attatggctt gtcggcttcg tgctatacca ccttctctct taccaccaag
     1201 tgcatttact actcggctta accattccag tgatgttagt gatttttctc agcaccattt
     1261 ttgtgaggag gttcacaaaa tagaaagata agcggttcaa ttggagtgaa aatttgctaa
     1321 aattgagtcg cttgttttaa acaatgactg ggtagtaaca ccacaagctc aacttgccta
     1381 ccacaccatt aacggtaaag ccgatgagga acggttaaat cttttagttg cccgtgccgg
     1441 cgtaagagta gcaaaaggct ttgccttgaa taatggttgg aatctgcaac cttatgcgga
     1501 agtaaacggc attgctgaaa aaggccaata gtacgaaagt gcaagtaaac caatatcagt
     1561 ttggagttgc cgaaaacaag ggcagagtgc aaggttcact tggcttgagt gcaggaaacg
     1621 gtaatcaccg tttaggttta gaggcatcag ttgcttcagg taagaaactg aaacaaccat
     1681 ttaatgtatt agtaaattat cgttatcaat ggtaatttaa gagggcaggt gaaaacctgc
     1741 ccttaaattc aataattaat ctatcttccc acaatttaaa cagtaaagat acatctgttt
     1801 cggatcgtta aaacgtgaaa gagtctctag ctgttgttgc ggttgcttca acagcggtaa
     1861 atcaagccaa tttgccacgc taaattgtaa ttgagctttg aaatcacggg caagcgtgcc
     1921 aaataagctg tcatcttgct ccgcaaacca ttgtaagccg aactgttcga ctttaggttg
     1981 gttttccgct tcacgttttt ggttgatcaa ttcagataac gcattatatg ccacattaaa
     2041 attgcccaac tcatcaacca atccgtgttt taacgcatca gttcccagcc acacttgccc
     2101 ttgagccact ttatctacca cctcttttgg cattttcctg ccacgagaaa ccagctctaa
     2161 aaaacggtcg tagccgtttt caatcccaat ttgcagcact tctgcctgct cttgcggtag
     2221 gctttttaaa ccggcttgtt gtgctagggc agaagtcgct atgccgtctt cgctcacacc
     2281 gagattttta gcggtttttt caaaagttac tgctaaaccg aaaataccga tagagccggt
     2341 aagcgtattc gggctagcaa taattttatc gctggtagcc gcaatccaat aaccgcccga
     2401 tgctgccatt ccgcccattg aagcaaccac cggtttgccg gcttgttgaa aggcttccac
     2461 ttcttggcga atcagttcag acgccacagc actgccgccg gggctgttaa tgcgtagaat
     2521 taagccttcc acatttttgt catctcgagc ttgttgcagt aattttacca gcgtctcgct
     2581 gcccacattc atttcgtcac tttcaccgcc agcaatcgcc ccttccacat tcactaccgc
     2641 aattttcgga gcattcactt ctgcaaagcg gtcgtttagt tcagtcatat aatctaaaaa
     2701 ctcaatctgg ttataactgc cttcgctgtt tttgccaaac tgtgcaatca gtttatcccg
     2761 actttcttgg ctggacacta actgactaac ccatttttga tttaatgcca gttgagcatc
     2821 atctccttta gcttgtttat atttttcaat gtaattggca ggcgataaat ccagtgcttg
     2881 cggttcaatg ttgcggtttt gagctagtgt tgccacagtt tgttgccaag tgccgtttaa
     2941 ccagcctcgg gcattttgtt tcgcctcctc agacatatca tcacgcataa acggctcaac
     3001 cgccgattta taagtaccga cacggaaaat atgcggttca gcctcaattt tatcgagcaa
     3061 ggttttgaaa taagtgttgg aataatttaa gccttttaaa tccaccgcac cgactttatt
     3121 gagataaatt tcatcagcaa aggaagcaag ataatattgt ttttgcgagt agtcttcgcc
     3181 tactgcaatc accggcttgc ccgattgttt gaagttgttg atttcattgc cgataaaatc
     3241 caatgagctg aaatcagcac tttgtagctt ttgcagatct aacaccaagc cggtaatttt
     3301 cgggtcattt ttagcgtggc taatcgcttg caccacatca aaagtcgaga ttttaaacgg
     3361 ctcggaacta cctaattccg attgcaccaa gcggtagaaa tcagtgaatt cttcccgatt
     3421 atctgctaaa taaccatcta aatttaaacg taacgcccct tgattgaaca ccggcttttg
     3481 cgtgctgcca gaagagctga taaatgcgac caccggcact aatagcaaaa taaacagaat
     3541 aaaaaagaga ttaatgacac actctctaat acaacggaag attcggtaaa atgcttttaa
     3601 aatcgttaac atcggaaatt cctaaaaata aactcagtcg gcacagtata aagcaatcgc
     3661 taaatgaaga aaatatgcta ttcttgactt gaacgcaaaa aattaacaaa ggaacgctta
     3721 tgcaaatctt ggatttatta caccatcgcc gttcaaataa aaaatttggc gataaagccc
     3781 cgaatgcaga acaactggaa caaattctaa aagccggctt gcgagtgccg gatcacgggc
     3841 ggttaaagcc ttatcgcttt gtggtgattg aaaaaagcgg tatgccacag tttggtgaat
     3901 gcttaaagtc tgcggtagca gaatttgata tgggcgagga acgcttaaaa aaagcggaaa
     3961 aattagctaa tcaagctcca atgattatcg gcgtggtagc aacattagac gacagcatcg
     4021 ccaaagtgcc aagttgggaa caaatgatta ccgcaggctg tgcaacttat gcgattcagt
     4081 tagcggcaaa tgcacagggc tttgatacgg tttggatcac caataaatgg attaacggct
     4141 ctgctcttcg ctccgctttt ggttgccaag aatctgataa aatcattgcc ttaattatgc
     4201 ttggctcacc ggcagagggc gagcaaattg cattagcccc tgaaagtgaa agtttagagg
     4261 ggtttgtacg ttatcttaag taagcaggtt aacagataaa agttagtaat aagggcgaaa
     4321 aacatttcgc ccttgctttt atctgttttc gccttacttc ttccagcctt ttaacgcatc
     4381 ggcaaaggca ttgttaccaa aagcgttgcg attgttgctt tgcaagcggt catttttctg
     4441 cgtatttttg cgaatttctg ctgaatttcg accgcttgtt ggctcttttt tatcggttgg
     4501 tttgtcgtct aaacgcatcg taagggcaat acgtttgcgg gcagcgtcca cctcaagcac
     4561 tttgactttc accacatcac cggctttcac taccgtgtgc ggatcttcaa caaaagagtt
     4621 tgagagcatt gaaatatgca ccaagccatc ttggtgtacg ccaatatcga caaaagcccc
     4681 aaagtttgcc acattggtca ctgtgccctc caaaatcata ccggctttga ggtcggtaat
     4741 ttcttccacg ccgtccataa acacggcggt tttaaactct ccacgagggt cacgccccgg
     4801 tttttctaac tctttgaaaa tatcgttcac cgttggtaaa ccaaactgct catccacaaa
     4861 atctttggcg ttgagcgagt gaattttgct gttgttaccc attaattctg caagtgttga
     4921 ggtagttgcc tgtaagattt tttctaccac cgggtaagct tccgggtgaa cgcttgaggc
     4981 atcaagggca tttttcccgc cgataattcg cataaaaccg gcacactgtt caaaggcttt
     5041 cggccctaaa cgaggcactt ttttcagctc tgagcggctg gtaaaacggc cgttttcgtc
     5101 acggaatgcc acgatatttt gtgccaatgt tttggtcatt cccgccaccc gagccagcaa
     5161 cggagcggag gctgtattca gatccacgcc cactgcgttt acacaatctt ctactaccgc
     5221 atcgagttta cgggcaagtt gggattggtt tatatcgtgt tgatactgcc caacaccaat
     5281 cgcttttggt tcgattttca ccaactcggc aagtggatct tgcaaacggc gggcaatcga
     5341 aaccgctccc cgtaatgaaa cgtctaaatt tggaaactcg ttagccgcaa attcagaggc
     5401 agaatagacc gatgccccag cctcactcac caccaccgtt tgcggtttat tctctttgat
     5461 ttctttaatc accgctttgg caaaacgttc ggtttcacga gaggccgtac cgttaccaat
     5521 cgcaatcagt tccacattgt gttgtttgat taagctgaaa atcgcaagct gtgcctccgc
     5581 ctctcgccca gtgtgagggt aaattgtggc ggtatccagc aatttaccgg tgttatccac
     5641 caccgccact ttcacgccgg tacgcaagcc cgggtctagc cccattgtat ttctcgcacc
     5701 ggcaggggct gccattaata atgccgagag attgcgggca aacacatcaa tcgcctcttc
     5761 ttcagctttt tcacgcaggg ttgccattaa ttcggtttct aaatgcagag ctgctttgat
     5821 tttccacgtc caagcaatca cttgctcacg ccatttatct gctggttgat tattaaaaat
     5881 cacgcccaaa tgctcacgaa taatttcttc acaatggctg gattttgtgc cttcttctgc
     5941 agtcggatcg gcatttaatg aaagtgataa caccccttca tttcgcccac ggaacatcgc
     6001 caaagcacgg tgggaaggca cgctgttaaa cggctcgcta tgagcaaaat aatcacggaa
     6061 tttctcgccc tcttcctctt tgccgtcaat cactttcgat tccaacgtgg cgtaagcggt
     6121 caaatattgg cgaagttttg caagtagctc agcatcttcg ctaaaacgct ccattaaaat
     6181 atatcttgcc ccgtctaatg cggccttggt atccgcaacg cctttttccg catcaacaaa
     6241 gtttgccgct aaggtttcag gatcttgttt cggatcattc cataaactgt ccgccaatgg
     6301 ctctaaccct gcttcaatcg caatttgccc tctggtgcga cgttttggtt tatacggcaa
     6361 atagagatct tccagctcgg tcttgctttc actgcttaga attttggctt ttaattcatc
     6421 ggttaatttg ccttgttctt caatcgactt caaaatggtt tggcgacgtt cgtccatttc
     6481 acgcaaataa attaagcggg tttcaaaatg gcgaagttgg gtgtcgtcta gcccgccggt
     6541 cacttcttta cggtaacgag caataaatgg aatggtattg ccctcaccta atagcgtgat
     6601 ggctgcaaga atttggtgtg agccaacgtt gagttcgccg gcaataattt ggctgatttt
     6661 tagatttaag tcagtcatta gttttccttc aataaatttg ctcgaggttt atccaatttc
     6721 tcaagctcgg tcaatgtttc ggcaattttt tgcagattcg gctctttttt cgcacataac
     6781 accatatttt ctaagctatc cgcttggtga tagtagggtg ctttgctttt gacagcctgc
     6841 tcaaattgct gataagcctg ttgaaattga ccctgtttac ataaaaaagt gccgtaatta
     6901 ttcagcacat cagggcggtt ttgactcaat ttcagtgccg tttggtaagc tttttcggca
     6961 ttttcagcat cacctgtttg ttggtaataa taggcaagca ccgaataagg gaggtaatct
     7021 ttttcatcgt gagccaaggc tttatcaata ttctgcttag cttttgggaa atcattttgc
     7081 tccagataag ctaaagccaa attaatacgg gcttttaccg cttccgagcg gttaaattcg
     7141 acttcagagg aagaaggggg aagagaagaa caagccgcta ataaaaaaat cagaatacaa
     7201 gcggtcattt ttggaaaaaa ttttgcaaac ataacgttct cctacaacaa agggcttaat
     7261 tttactgttc catcctcgct aagactagcg attttttgcg aagaagaaaa atttttcgtt
     7321 ttcatttttc gacgcagatc gcaaaaataa taatggggtg ttgtgctagg gtaaatggta
     7381 ggaatacact ctctatttaa tataagaatt tcattgaaaa aacttataaa acttataaaa
     7441 tagaggcaaa aatgaaaata gtggtattcc agcgtaaagc cttaaagcaa ttacgaaaaa
     7501 tcccaactgg tgcagaaatt cgccgaaaat gtgatgattt agcttacttt ccctactgtg
     7561 ccaatattaa ggcattaaca aatcatcaat atgattatcg ctttcgggtc ggaaattacc
     7621 gtattttttt caatattgaa ggcgaaacga tgaatattgt gtctatcgaa gaggttaaaa
     7681 aacgagatga aagaacttac taatattcaa tatttaaata atgaagaagg aaaacctgcc
     7741 tttgccgtac ttccgatagc aacacttgaa tggcttaagc agaaagcaaa tattaaaacc
     7801 gcaatagaga ccggtattcc atctgctgtt gctggaattg cattagataa taactactca
     7861 gctctgcgtg cttggcggga atatttaggt ttaactcaag aagaggtggc acaacgcttg
     7921 gggatttcgc aatcagctta tagccaacac gaaaatgcaa aaaatctacg aaaaacgacc
     7981 cgcataaaaa ttgcctatgc gttgggttta aatgaagagc aattagattt ctaataacaa
     8041 aaaggcagga acctctgcct tttcactatt cagattaatg ggttttaacc gcaataccct
     8101 gcccaaattt acgtttttca agggtacgtt tagtgcggtc aatcacatcg ccggcaagct
     8161 gtccgcaagc ggcatcaata tcatcgcctc gtgttttacg cactgttaca gtaaaaccgt
     8221 actccatcaa ggttttttgg aaacgatcca cccgagagtt ggagcttttt ccataaggtg
     8281 cttccgggaa cgggttccac ggaatcaagt taattttaca cggcgtgttt ttcaatactt
     8341 cagcaagttg gtgggcgtgg tcggtgctgt cattaacgtg atctaacatc acatattcaa
     8401 tcgtgacctt gccgtggttg gcgttagaaa cttcaaggta tttattcacc gaatccatca
     8461 acattttgat gttgtacttt ttattaatcg gcactaattc atcacgcagt tcatcgttcg
     8521 gagcgtgaag tgaaattgcc aacgccacat caatttgctc acgcattttg tctaatgccg
     8581 gcaccacacc tgaagtggaa agcgtgacac gacgtttaga taagccgtaa gcaaaatcat
     8641 ctaacatgat ttccattgcc ggaatcacgt tattcatatt cagcaatggt tcacccattc
     8701 ccatcatgac cacattggta atcgggcgaa caccggtcac gccaaaattg ccgataattt
     8761 tagaagcacg ccacacttgc ccgataattt ccgccacgct taagttacgg ttaaagcctt
     8821 gttgagcggt tgagcagaag gtacaagcca aggcacagcc tacttgtgaa gaaacacaca
     8881 gggttgcacg gtcatcttca ggaatatata ccgtctcgat ttgctgatcg cccacttgca
     8941 ttgcccattt aatcgtaccg tctgcagaac gttgttctac cgccacttca ggggctttaa
     9001 tttcggcaat acgctttaat ttttcacgca atactttatt gatattgctc atattatcga
     9061 aattttcttc gccaaaatgg tagatccatt tcattagctg gtcggcacgg aaaggttttt
     9121 cacccatttc tgcaaacagt tctcgcattt cttgacgatt aagatttaat aaattgattt
     9181 tttcttttgg ttgttcagca gacccacagc ctgcattggt aatcagttct gacataaagt
     9241 tgaaagcctc gttattacac ggattacggc aaaaggttag ggcaaatgcc cttattttgc
     9301 gattggattt taaagcgtgc aattttactg gctcttgagg gattaggcta gtaattttta
     9361 atcaaaagag ttgaattgcc tgagtaaact tatcaagaat aaccttataa aaacaagact
     9421 atttacatca ttcccaatgt tctatagaat agctttcgat aattctcatt ctcaataaca
     9481 aaaggaggct aaaaatgaaa ctatcaacaa ttaaaaaagc attggtatta acctttggtt
     9541 taagtgcagc gatggcaatg gcagagccgt ttaaagtagt taccactttt accgtgattc
     9601 aagatattgc acaaaatgtc gccggcgata aagccactgt ggaatccatt actaaaccgg
     9661 gtgctgaaat tcacgattat caaccaacac caaaagacat tgtaaaagct caaaaggcag
     9721 acttagtgtt atggaacggt atgaaccttg agcgttggtt tgaacgtttc tttgaaaatg
     9781 taaaaggcaa accggcggtt gtcgtcaccg aaggcatcac cccaatgccg attaccgaag
     9841 gtgaatataa aaatctacca aatccgcacg cttggatgtc caccgataac gcattaattt
     9901 acattgaaaa tattcgtgcg gctttagtaa aatatgaccc acaaaacgca gacacctata
     9961 atgccaatgc aaaagcctat gctgaaaaag taaaaggtat tgctgagcca ctccgccaac
    10021 gtttagcttt tattcctgag cagcaacgtt ggttagtaac cagtgaaggg gcatttagct
    10081 atttagcgaa agactacaac ttaaaagaac tttatttatg ggcaatcaat gcggaagagc
    10141 aaggttcgcc aaaacaagtg aaaaaagtga ttgatggtgt aaaagccaac aaaatccctg
    10201 ttgtgtttag cgaaagtacc gtttccgaca aaccggcaaa acaagtggcg aaagaaaccg
    10261 gtgcattata cggtggcgta atttatgtgg attcactttc agagaaagat ggtccggttc
    10321 caacctattt agatttgctt aaagtgacta tcggcaccat tgtcgaaggg ttcgaacgaa
    10381 gcaaaaaata ataagattta gcaatgacaa ctgtttcagc ctccatttcc gtcgaaaatt
    10441 tatcggtccg ttataataac ggacatatcg ctttgcaaga tgtctcgttt catctggaaa
    10501 acggcacaat ttgtgcatta atcggcgtga atggaggtgg aaaatccact ctgtttaaaa
    10561 gtttaatggg gctggttaag ccattaaccg gctctgttaa gctgagtcag ctcccgattg
    10621 ctaaagccct caaacaaaat ttggtttcct atgtgccaca aagtgaagaa gtggactggc
    10681 aatttccagt ttcggtttac gatgtggtaa tgatggggcg atacggctat atgaattttc
    10741 tacgccgccc aagccaaacc gataagctca aagtagagca agcaatgcaa cgccttgatg
    10801 ttttacatct caaagacagg caaatcggcg aactttccgg cgggcaaaaa aaacgggtat
    10861 ttttagcccg tgccttagct caagaaagta aaatcatctt acttgatgag cctttcaccg
    10921 gtgtggatgt taaaaccgaa aatgcgattg ttgagctact taaacagctt aaaaatgagg
    10981 ggcatttgat tttagtttcc acccacaacc ttgccagcgt accaagcttt tgcgatcagg
    11041 ttttgatggt caatcgcacg ctactggcaa aaggtaaaac ggaagaaatc tttaataatc
    11101 aaaatttaga acgggtgttc ggcggtttat tgcattatca aaagtagtgt ttcagcaaca
    11161 aatcacaaac aaatagggag gcaaatgcct ccctattttt gtatcaatga atcttagaat
    11221 atgcaaaact gaggaaaaaa atggccgctt gcacgagaat aatcgtcggc cctgttgcgg
    11281 catcaatatg gtagctaagc aacgtgccga ataccgatga aaatatcgca acgcctaagg
    11341 caatcagtaa catactatca aaccgcttag tcagcaggta agcagtgatc cccggggtaa
    11401 tcaacattgc caccactaaa atcacgccca ccacttgcat tgcactaata atagttaaag
    11461 caagcaagga aagtaaacca taatgcaata atctaaccgg taagcctgct actttggctt
    11521 gattaacatc aaagcaatag agcaagaagt ctttgcgttt cgccaccatc accacaaagg
    11581 taaagaggct tatgagtaaa gtctgaagca aatcgctttt ggttacgcct aatagattac
    11641 caaataaaat atgggttaaa tgctcaccgg tattaaaggc ggtaaacagc aagatcccaa
    11701 aggcaaacat acctgaaaat acaatcccca ttaccgtgtc ttctttcaag cgactatttt
    11761 cttttaaata gcccaccgat aaggcacagc ctaatcctga aaaaaacgcc cctattgaga
    11821 gtggtaaccc ggcaagtgag gaaatcacca caccgggcag aacagcgtgc gaaattgcat
    11881 cgcccattaa agaccagcct ttcagcacca gatagcaaga taaaaccgca ctcacgccag
    11941 caactaaaat tgcggtaatt aacgcaattt gcataaattc aaattgcaac ggctctaaaa
    12001 tccaattaag cattttgaac ctccacacgg tgcaatttcc gcttttgagt aatcaagcca
    12061 tatttaggag caaagcagaa tgcgaataag aaaattaagg tttgtaagca cacaattacc
    12121 gcaccggttg caccgtctaa aaagtagctt aaatatgccc caatccctga tgtcagcgtg
    12181 ccgataagca ttgcgatcag cactaatcta gggaaacggt cggtaagtaa ataagcagtt
    12241 gctcccggcg tgacgaccat cgcaataacc aaaattgccc caaccgtttg cagagccact
    12301 actacacagg cacttaataa ggtaaagaaa atcactttat agcgtaatgg cgaaagcccg
    12361 actgacaatg cgtggtgttc gtcaaaaaag acgagtagca aatccttcca aaataggatt
    12421 aaacccagta acgaaatcgc aataatcagt accacctgcc acaagtcatc atcagcaatg
    12481 cctaaaatat ttcccattac aatcgaatta acatcaaccg atgttggatt gagggaaaca
    12541 ataaacatac caagcgcaaa aaaagtcgtg aaaataaagc cgataaccgc atcttcttta
    12601 attttcgtca ggctttttac ccaaagaatc gataatgccg ccaaaatccc ggaaaaaaac
    12661 gcaccaaagg cgtagggcaa ttttagtgca taagcgatgg caacacctgg cactacagcg
    12721 tgtgataagg catcaccaat caaagaccag ccttttaaca tcaaaaaggc ggaaagaaaa
    12781 gcacaaacta accccacgcc catactgaca aaaatcgctt tttgcatata gttgtaggta
    12841 aaaggctcta ataaaagatc aagcataaaa tcctaacaag ttgttgataa tagttcgcat
    12901 atactatcat ataaactcct tttcttgtat ggattaaact taaatatcta ctttaaattg
    12961 ataaggatca ggatagtaat aacgaaaacc aagctcttgg caaattttgt ctgcttttat
    13021 cagccttaca agcggttgat tttcttcaga aaaatgcaaa tccgctaacc caaaacgttt
    13081 tgccatttca ctataataat ctttacggct tgggtgctgt tcggcacata aatgaaaaat
    13141 acgctgcccg ttcggctgct ccaacagcaa tgaaatggct gcaatacagt cctctaaatg
    13201 cactaaattg accggctgcc ctgctccact caagttctgt tttcccgcca aataaaaaac
    13261 cgggtgccgt tttttgccca ccaagccagc taaacgcaaa atatcacaat gaattggctg
    13321 gctcagcaac caattttcca tttttactaa taggttagca gaatcaacca ctgcattttc
    13381 atcaaaaacg cccgatttca ttggcaaaac cgaagtcgag ctggtgaaaa ccagatgttt
    13441 caagcccttt tctattgcaa gcgaaactaa tgtttgaata ccgcaaccgt aattttcagg
    13501 gcgaatttgg ctaaggggca aggcaatcac taacgcatca gcagcaagta attgctcgat
    13561 ttcgcttgtc actgctaacg catttaaatc tagaggataa cattctatcg ctaattctcc
    13621 gaccgttcgc ttagtacctg caacacgcca gcccttattt tgtaatgctt cagctaaagg
    13681 tagtccgagc cagccaagcc ctaaaatggt aatttttttc atcatttacg tttctcaaga
    13741 ttaaaattgg aatgagggga aattcaggta caaaaaaagc acctcgtggt gctatctatt
    13801 ttatttcta
//
LOCUS       DANKBV010000057        13691 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.057, whole genome shotgun sequence.
ACCESSION   DANKBV010000057 DANKBV010000000
VERSION     DANKBV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.057"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            79..1548
                     /locus_tag="PWY43_001371"
     CDS             79..1548
                     /locus_tag="PWY43_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2136247.1"
                     /translation="MMDGLSYNASSQQIYNRLVEFVPGTTDIEPALAESWEISEDGLT
                     YTFKLRKGVKFHSNKDFTPSRELNADDVVFSFNRQLDKNHPYHTVSNGTYPYFNAMKF
                     PTLLKSVEKVDDSTVRITLTKRDASFLSSLGMDFTSIYSAEYADKMMKAGIPEAVDTT
                     PIGTGPFVFSGYVLDQASRYVANKEYWKGKPDIDRLIFEIVPDATTRYAKLQSGQCDL
                     MDFPNATDIEKMKNDPKINLLSQPGLNIAYVAFNTEKAPFDNVKVRQALNLAVDKKAI
                     IDVVYQGNGFAAKNPLPPTIWGYNDSLADSEYNVEKAKQLLAEAGFPNGFETELWVQP
                     VVRASNPNPRRMSEIIQADWEKIGVKAKLVTYEWGDYIKRTKAGELTAGTYGWSGDNG
                     DPDNFLSPLFGTPNIGNSNYARFTNPELDALLEKALVSSDKAERTKLYEQAQVILREQ
                     APWINVAHSINFAPTSKRVEGYKQSPFGYTYLYPVKIVD"
     gene            complement(1597..4542)
                     /gene="ptrA"
                     /locus_tag="PWY43_001372"
     CDS             complement(1597..4542)
                     /gene="ptrA"
                     /locus_tag="PWY43_001372"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDL2136248.1"
                     /translation="MKLSFLTQFVAAAFALNVMTYPAFAEVAKNSEKTTASQPQSQLG
                     FELIKQSINKSPNDKAIYQGIKLDNGMEVLLISDSQANKSLMSAIIPIGSMDDPISQQ
                     GLAHYLEHMILMGSKNYPETNSLDGFLTKNGGMNNASTASHRTAYYLQVNNEAFSEAV
                     TRLADTLAFPLLLETNAKKEVNAVNAEMVRAKSHDGHLLHSVNLATANPAHPATKFAV
                     GNKETLSDKPDSKLQTELEQFYKQHYSANLFKVVLYSNQSIEQMAKLAANTLGKMENK
                     HFSIPQTAEPRYRDEDKSVFIQYKPVKSNKMLVIGFDFPNDEMQFKHKTGKYIAYMLN
                     NNTDGTLSDYLIKQGLSDGGIEAVSSANIGRDRGSFDIYIELTDKGLSEQDQIISLVF
                     QQIEKIKQEGIQESYFSEVKESLKQDFQHLQVEKNMDFVEFIADQMLHFPLEHVIDQP
                     YLTETMDNEAIKAKLAAMNIDNARIMLVSDTAKTDKKTPYFEAGYSVAKITQEQKQKW
                     LDFSKNPQLNLPELNPYFTTDFTLNKVETGLTKPKLINDQAGNLIYTMPSVYFTNEPK
                     AKIGVGFNIRPRKDDLKSLTAASILGYMNNLAQTKLDFQSSVAGMNLSLAPAANGLAL
                     SAEGYTQNLAKLMLDSVKQFSRFEFNEDVLAQAKQRLHQELDRNEKENSLNQANAALS
                     RFASYPYFDVPKQREMIDSITLADIEQLRQRILQQSSGFKLLSVGNLSAEQVSALAED
                     LQAVIKNQKSANATGQYVDVSTSQRKINVIKSVPNEDNALLMAFMPNGYNELESLTRA
                     KLLRSIISRWYFDDLRTDKQLGYVVYATDNIIGKTAGIQFMVQSPNTTPAGILEHNER
                     FFTQSFERLKNLSDEEFGKYRDSLVEKLQYKPESLAEEFSEFGSDFSRNNPKFDRLQQ
                     VIELTKALTKADIVAFYQKAVIEKQGFVFVSQAVGTKTKAEDVAKFDGFEQIESIEKL
                     QREFEIKDY"
     gene            complement(4678..7968)
                     /gene="recC"
                     /locus_tag="PWY43_001373"
     CDS             complement(4678..7968)
                     /gene="recC"
                     /locus_tag="PWY43_001373"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDL2136249.1"
                     /translation="MFTLYQSNQISSLAEMLVKIQQVNPLEDPFEPETILIQSQGMAQ
                     WLQMQIAELNGVMGNCDFLYPTTFLWQQYRLLFPELPKENIFERSSLVWRIMRLLPSC
                     LTLSEFAPLKRYLGDQPNKEQYQLKLYQLSAKIAELFDQYLVYRPHWLVSWEEGNLQA
                     IENEIKIATAAKPIDFDLIRADMKWQSHLWQQIVSDLQSESDEQVFTTSHRAYLQQNY
                     FAKLDDLTETEKAKLPKRIFIFGISSFSPLQLAVFKKLSEHCDIHLFFFNPSEKYWGD
                     SVEEKVWQKLALSHKISQDDLDNLLAEQRNKLLNLWGKQGRDFLAQLIEFEPNTIDVF
                     VEPEENSNLNKLKKQIFSLENDATITDLTADNSLQIHSCHSPMREVEVLHNQLLNLFE
                     QDSTLLPKDIIVMSPDINKYAPYIDAVFSRYNTGNRDPRYIPFSLSDQAFTQVDPIIH
                     SFLQLLAMKESPFNAEELFDLLEVNAIQTRFGFSTEDIHTLRVWVRNAGVRSGVEIEQ
                     PHWQNYNSWENGLNRLLLGSSLKEEQGIWENTLAFGDSYGLNAELVGRLASFIEALLA
                     WVNFLQTPQPLAAWQQAIKNLTADLYQENAENAASILLLNNVNDQIIEQISATHFSDD
                     LQIEVLALLFEQQLSQHSQQLNFLVGRVNFATLLPMRAIPFKVICLLGMNEADFPRQQ
                     QINSFDLMQYAPKKGDRAKRDDDRYLFLEALLSAQQMFYISYIGQSQKDGKEMLPSIL
                     VSQLLDAMNEALTPECKQQLSDEVEVLVRKQPLSVFSKKNFTHPRDHAYNAEWIIKPT
                     VAVQDFLSEPLTNESVASLELDELISFVQNPIRFFFLKVLGISFNTYDDTIDESEIFE
                     MSGLDNYRILNELLTINLAQQQRFFAEEQLKGNLPASYFAQIATQDLIGKITEMQAAL
                     SHYLASESRILSVNREVELERQTLTLNGNIANLYQNEVVLWRVGHLRDKDKIQTWIYH
                     LLLCAYFPEKTVKFYYREGEQVGVLSFETVSQIEALSQLSIYLNAYLDGLSQAQLVIY
                     DRIEEYLKGDGTNSEQVLIDLAEKDERDGSYLHRLLAQTKELDSQAIHQRTQSWFSLM
                     NQKIAKGD"
     gene            complement(8017..8310)
                     /locus_tag="PWY43_001374"
     CDS             complement(8017..8310)
                     /locus_tag="PWY43_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5374 domain-containing protein"
                     /protein_id="HDL2136250.1"
                     /translation="MIKYKAETALSLLVAITLFAIVVLSFSQWQSAQNHRLNQHFQQQ
                     QAVMILENQLALKLAGLDCESSITQNQIRYQIQCSGNRLTIRFPLGKIELNND"
     gene            complement(8303..8986)
                     /locus_tag="PWY43_001375"
     CDS             complement(8303..8986)
                     /locus_tag="PWY43_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2572 family protein"
                     /protein_id="HDL2136251.1"
                     /translation="MNKYKNASALLVSLILISTILSVLFLSKEKWLGQQNITHFYREK
                     YLSDQYNFLALYPQDKTQICQKFQKEQISQEDLSDKQFRFLQYQFACQFHSLFKDKKP
                     TKEKYIHFTRLADYLDLSNVPNQEIYTIHSLAELPSSSEREPKIVLAKNAINENLPQD
                     FYGIVITDYLFDITGKKMYGTLYSSYDNAREERNLTFKKEVLAELEARYSAWRYLPYS
                     EHLMGAVDD"
     gene            complement(8989..9615)
                     /locus_tag="PWY43_001376"
     CDS             complement(8989..9615)
                     /locus_tag="PWY43_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136252.1"
                     /translation="MASALGAFLLLLVASSYADFYYTQTKQKEQLYLQAEAHQLLNYF
                     QQHFQHIGYQGSHRTDSNFDLFQQAGKSLNIVHKNCIIGFYDLNQDGCLGKRKTKTAA
                     CKLGTVNNTREVLKEIVGFKVENNEIYTFSSQLDNCVKEQCQTLLENCDGNWSKFTEA
                     NNFKTKKLDFSWRQEDNLLKIELEIESPKQKGLIYTAVRYVFILNNTR"
     gene            complement(9647..10168)
                     /locus_tag="PWY43_001377"
     CDS             complement(9647..10168)
                     /locus_tag="PWY43_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HDL2136253.1"
                     /translation="MYKAITLIEILTTITIMVIAVYFISPVIFTLQDRIALHSEIENI
                     QSFIQQIQTKARYTKRNYTLTLSQRNNQWCMIAVIKPINTSKQIICDCLNKHFCDLGK
                     EYHLYTPTHQQIVLKNKSLYPKSFINVDGVSGQLESKCIKLSLNKENEILQFEQLGRV
                     YVAAKNKRSTCKD"
     gene            complement(10269..10718)
                     /locus_tag="PWY43_001378"
     CDS             complement(10269..10718)
                     /locus_tag="PWY43_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HDL2136254.1"
                     /translation="MLAKLKWNQLACVTLAFCSASVFSAHHEIKMLDNNAEGSMVFEP
                     GYVKAEVGDTVTFIPSHKGHWVESRSIPEGAEKFLSKEDEEFTITLTHEGVYVYYCPP
                     HRTMNMAGIIQVGQALNLEATQKEVEKIEKRTKENKGRLFEYLEQVK"
     gene            complement(10778..11449)
                     /locus_tag="PWY43_001379"
     CDS             complement(10778..11449)
                     /locus_tag="PWY43_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2136255.1"
                     /translation="MNYAVNKTEILTSVYQGSATPAKNPMPSTIWGYNNEVAGYEFNP
                     EKAKSLLKEAGLENGFETEIWAMPVSRPYNPNARRMAEMIQEDWKAIGVEAKIVSYEW
                     GEYLKRMGNGEHATGMIGWTGDNGDPDNFLNALLSCNAVEQGSNYAGFCNPEFDRLIN
                     EAATISDQNKRSDLYKKAKFIFNEQAPWLPIAHSTVYFPVRKEVKGYVVSPFIAHNFY
                     GVELE"
     gene            complement(11446..11793)
                     /locus_tag="PWY43_001380"
     CDS             complement(11446..11793)
                     /locus_tag="PWY43_001380"
                     /inference="COORDINATES: protein motif:HMM:NF012706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136256.1"
                     /translation="MQIAGTPEKLDLEPIGTGAFQFYGYQKDAAVRFTAFEHYWEGKA
                     KIDRLVFSITPDASVRLAKLEKNECQAMPYPNPSDLPALKANKDIQLFEKSGLNIGIL
                     VSPLLITLKFVKH"
     gene            complement(11684..12347)
                     /locus_tag="PWY43_001381"
                     /pseudo
     CDS             complement(11684..12347)
                     /locus_tag="PWY43_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
     misc_feature    complement(12367..12512)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(12689..>13691)
                     /gene="mglC"
                     /locus_tag="PWY43_001382"
     CDS             complement(12689..>13691)
                     /gene="mglC"
                     /locus_tag="PWY43_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDL2136257.1"
                     /translation="LQQNKTLDFFKQNAIYFVLLILLIVIIVQDPSFLSLRNFSNILS
                     QSSVRLIIALGVAGLIVTQGTDLSAGRQVGLAAVISATLLQSMENMNKVFPNLSEIPI
                     PVVVLTVCAIGAVIGLFNGFVVAYLNVTPFIATMGTMIIVYGINSLYYDAVGSSPIAG
                     FSDDFSTFAQGFFRFGDFRLSYITIYAVIATIIMWTLWNKTRFGKNVFAIGGNPEAAR
                     VSGVNVPLNLMGIYMLSGIFYAFGGMLEAGRIGSATNNLGFMYEMDAIAACVVGGVSF
                     AGGVGTIIGVVTGVLIFTVINYGLTYIGVNPYWQYIIKGGIIILAVAIDSLKYAKKK"
BASE COUNT         3957 a         2898 c         2622 g         4214 t
ORIGIN      
        1 gcagcagcaa aaccgcaaac cgagaaaacc tttgtaaact gcgttagccg ttcgccaacc
       61 ggttttagcc cgattttaat gatggacggt ttatcttata acgccagctc gcaacaaatt
      121 tacaaccgct tggtggaatt tgtgccgggt acaaccgata ttgaaccggc attggcagaa
      181 agctgggaaa tcagcgaaga cggcttaact tacaccttta aattacgcaa aggcgtgaaa
      241 ttccattcaa acaaagactt cactccaagc cgtgaattga atgcggacga tgtagtattt
      301 tcatttaacc gtcagttaga taaaaatcat ccgtatcaca ccgtctctaa cggcacttat
      361 ccgtatttca acgcaatgaa attcccgaca ttattgaaat cggtggaaaa agtggacgac
      421 agcaccgttc gtattacctt aaccaaacgt gatgcgtcat tcttatccag ccttggtatg
      481 gatttcacct ccatttattc ggcggaatat gcggataaaa tgatgaaagc cggcatacct
      541 gaagcggtag ataccacgcc aatcggcaca ggtccgtttg tgtttagcgg ttatgtgcta
      601 gaccaagcca gtcgttatgt agcaaacaaa gagtattgga aaggtaagcc tgacattgac
      661 cgtttgattt ttgagatcgt gccggatgca acaacccgtt atgcgaaatt acaatccggt
      721 caatgtgatt taatggactt cccaaatgca accgacattg agaaaatgaa aaatgatccg
      781 aaaatcaatt tactttctca accgggtttg aacattgctt atgtggcatt caataccgaa
      841 aaagctccgt ttgataacgt gaaagttcgc caagcgttaa acttagcagt ggataaaaaa
      901 gcgattattg atgtagttta ccaaggtaac ggttttgcgg cgaaaaaccc acttccgcca
      961 accatttggg gctataacga cagcttagcg gattcagaat ataacgtgga aaaagcgaag
     1021 caactattag ctgaagcagg cttcccgaac ggttttgaaa ccgaattatg ggtgcagcct
     1081 gtggtgcgtg cttctaaccc gaacccacgc cgtatgtctg aaattattca agccgactgg
     1141 gaaaaaatcg gcgtaaaagc aaaattagtc acctatgaat ggggtgatta catcaagcgt
     1201 accaaagccg gtgaattaac cgccggaact tacggttggt cgggcgataa cggcgatccg
     1261 gataacttct tatcgccact attcggcacg ccaaatatcg gcaacagtaa ctatgctcgc
     1321 tttactaacc ctgagttaga tgcgttatta gaaaaagcat tagtctcatc tgataaagca
     1381 gaacgtacta agttatacga gcaggcacag gttatcttaa gagagcaagc tccgtggatt
     1441 aacgtggctc actcaattaa ctttgcccca accagcaaac gtgttgaagg ctacaaacaa
     1501 agtccgttcg gatatactta cttatatccg gtgaaaattg tggattaaaa aatgaggggc
     1561 gaaaattttt cgcccctaac tctctctaat aactctctaa taatccttaa tctcaaactc
     1621 tcgttgtagc ttttcaatgc tttcgatctg ttcaaaaccg tcaaatttcg caacgtcttc
     1681 agctttggtt ttggtgccga cagcttggct gacaaacacg aagccttgct tttcaatcac
     1741 agctttttgg taaaatgcca caatatctgc tttggtgagt gctttggtta attcgatcac
     1801 ctgttgcaag cggtcaaatt tcgggttatt tctgctaaag tctgaaccga actcagaaaa
     1861 ctcttccgct aaagactccg gtttatattg cagtttttcc actaggctat cacgatattt
     1921 tccaaattcc tcatcagata gattttttaa acgttcgaaa ctttgtgtaa agaaacgttc
     1981 gttatgctct aaaatccctg ccggtgtggt attcgggctt tgtaccataa actggatacc
     2041 ggcggttttc ccgatgatat tatctgtcgc ataaaccaca tagcctagtt gcttatcagt
     2101 acgcaaatca tcaaaatacc aacgggaaat aatgcttctg agcaatttgg ctcgagtaag
     2161 gctttccagc tcgttatagc cgttcggcat aaatgccatt agcaacgcat tatcttcatt
     2221 cggcacgctt ttaatcacat taatcttacg ctgactggta ctcacatcca catactgtcc
     2281 ggtagcattt gcagattttt ggttttttat gaccgcttgt aaatcctctg ccagagcaga
     2341 aacctgctca gccgacaaat tccccaccga taacaactta aagccgctgc tctgctgcaa
     2401 aattcgctgg cgtagttgct caatatccgc aagggtaatg ctatcaatca tctcacgctg
     2461 tttcggcaca tcaaaataag ggtaagaggc aaaacggctt aatgctgcat tagcttgatt
     2521 aaggctgttt tctttttcgt tacgatccag ctcttggtgc aaacgttgtt tagcctgtgc
     2581 caacacgtct tcgttaaact caaagcggct gaactgtttt acgctgtcta gcattaattt
     2641 agccagattt tgggtataac cctctgcact taaggctaaa ccgtttgccg ccggagctaa
     2701 acttaaattc atacccgcca ccgaagactg aaaatctaac ttggtttggg ctaaattgtt
     2761 catataaccc aaaatagacg ctgcggtcag gctctttaag tcatcttttc tcgggcgaat
     2821 attaaagcca acgccgattt tagccttcgg ttcattggta aaatacacgc tcggcattgt
     2881 atagattaaa ttccccgctt ggtcgttaat caatttcggt ttagttaagc cggtttcgac
     2941 cttgtttaag gtaaaatcgg tagtgaaata cgggttcaat tcaggcaaat tgagctgtgg
     3001 atttttactg aaatccagcc atttttgctt ttgttcttgc gtgatttttg ccaccgaata
     3061 acctgcttca aaataagggg ttttcttgtc ggttttggca gtatcgctga ctaacataat
     3121 gcgggcatta tcaatattca ttgccgccaa ttttgcttta attgcctcgt tatccattgt
     3181 ttcggtcaga taaggctgat cgataacgtg ttctaacggg aaatggagca tttgatcggc
     3241 aataaattcc acgaaatcca tatttttttc cacttgcaaa tgttggaaat cctgcttcag
     3301 gctctctttc acttcgctaa aatagctctc ttgaatgcct tcttgtttga tcttctcaat
     3361 ttgttggaaa accaatgaaa taatttgatc ttgctcgctc aaacctttgt ctgtcagttc
     3421 aatatagatg tcaaacgagc ctcgatcccg cccgatattg gcactgctaa ccgcctcaat
     3481 accgccgtca gacaacccct gtttaatcaa gtaatccgaa agcgtgccgt cggtattatt
     3541 gtttaacata taagcaatat atttaccggt tttgtgctta aactgcattt cgtcattcgg
     3601 aaaatcaaag ccaatgacta acattttgtt ggatttcacc ggcttatatt gaataaatac
     3661 cgatttgtcc tcatcacgat aacgtggctc agcggtttgc ggtatgctga aatgtttgtt
     3721 ttccattttg cctaaagtgt tggctgccaa ttttgccatc tgctcaatcg attgattgga
     3781 ataaagcacc actttaaaca gattcgcaga ataatgctgt ttataaaatt gctccaactc
     3841 ggtttgtaat ttgctatccg gcttgtcaga taacgtctct ttattgccta ccgcaaattt
     3901 ggtagcagga tgagccgggt ttgcggtggc taaattaaca ctgtgcaata aatgcccatc
     3961 gtgcgattta gctcgcacca tctccgcatt gaccgcatta acctctttct tggcgttggt
     4021 ttccagcaat aacgggaaag ctaacgtatc cgccagtcgt gttaccgctt cgctaaaggc
     4081 ttcgttattg acttgtagat aatacgccgt gcggtgagaa gcagtcgagg cattattcat
     4141 accgccattt ttggttaaaa aaccgtctaa gctgttggtt tccggatagt ttttcgagcc
     4201 catcaagatc atatgctcaa gatagtgggc taaaccttgc tgagaaatcg ggtcatccat
     4261 tgagccaatc ggaataatgg cagacatcaa cgatttattc gcttgactgt cggaaatcag
     4321 tagcacttcc attccattat caagtttaat accttgataa atcgctttat cattcgggct
     4381 tttgttaatg ctctgtttaa tcagctcaaa accgagttgg gattggggtt gactagcggt
     4441 cgttttttca gaattttttg caacttccgc aaatgcaggg taagtcatca cattgagggc
     4501 aaaagccgct gcaacgaatt gggtaagaaa agataatttc atacatctcc ttttgaaatt
     4561 agcattgtag taataaattg acagtaaaat acggaaaaaa ttctattttt ataggtaggc
     4621 gaattattat tcgccttctc attttgcaaa ttttcagcca aaaacgaccg cttgtagtta
     4681 atcgcctttc gctattttct gattcattaa gctaaaccag ctttgagtac gttggtgaat
     4741 cgcttgagaa tccagctctt tagtttgagc gagaagccga tggagatagc tgccgtctcg
     4801 ctcatctttc tctgctaaat cgataagaac ttgttcgctg tttgtgccat cacctttgag
     4861 atattcctca attcggtcgt agatcactaa ctgggcttgc gataatccgt ccaaataagc
     4921 attcaaataa atgcttaatt gcgataatgc ctctatctgc gagacggttt caaagctcaa
     4981 cacaccaact tgctcaccct ctcggtaata gaatttcacc gttttttcag ggaaataggc
     5041 acacaataat aaatggtaga tccaagtctg aatcttgtct ttatctcgta aatgccctac
     5101 tcgccatagc accacttcat tctgatatag gttggcaata ttgccgttta gggtgagggt
     5161 ttggcgttcc agctcaacct cccgattaac actcaaaata cggctttcgc tggctaaata
     5221 gtggcttaac gccgcttgca tttcggtaat tttgccgatc aaatcttgtg tggcaatttg
     5281 agcaaaataa gaagcaggta aattgccttt gagttgctct tcggcaaaaa aacgttgctg
     5341 ttgtgctaaa tttatcgtta ataattcgtt taaaatccgg tagttatcca gccccgacat
     5401 ctcaaaaatt tcgctttcat caatggtgtc atcataagtg ttaaacgaaa tccccaacac
     5461 ttttagaaag aaaaacctaa tcgggttttg tacaaaagag attaattcat caagttctag
     5521 cgaagccacg ctttcattgg ttaaaggctc actaagaaaa tcttgtacgg caacagtcgg
     5581 cttgataatc cactctgcgt tataagcgtg atctcgtggg tgggtaaaat tctttttgct
     5641 aaacacactt aaaggttgtt ttctgaccaa aacttccact tcatcgctta attgttgctt
     5701 gcattcaggc gttaaagctt cattcattgc atctaacagt tgggaaacca aaatcgacgg
     5761 tagcatctct ttgccgtctt tttgcgactg cccgatatag ctgatgtaaa acatttgctg
     5821 agcggaaagt aaggcttcta agaacaagta gcggtcatca tcacgtttgg cacggtctcc
     5881 ctttttcggg gcgtactgca ttaaatcaaa gctattgatt tgttgttggc gagggaaatc
     5941 tgcctcattc atacccagca agcaaattac cttaaacgga atcgcccgca tcggaagcaa
     6001 ggtggcaaaa ttcacccgtc cgaccaagaa attaagctgc tgtgaatgtt ggctgagctg
     6061 ctgctcaaac aataaggcta aaacctcaat ttgcagatcg tctgaaaaat gggttgcgga
     6121 aatttgctcg ataatctgat cattcacatt attcaacaat aaaattgatg cagcattttc
     6181 ggcattttct tgatacagat cagcagtcaa attttttatt gcctgctgcc acgctgccaa
     6241 cggctgtggc gtttgcagaa agtttaccca cgctaataaa gcctcaataa aagaggcaag
     6301 tcgccccacc agctcggcat ttaagccata gctatcccca aaggcaaggg tgttttccca
     6361 aatcccctgc tcttctttca ggcttgagcc aagcaataaa cggtttagcc cgttttccca
     6421 cgaattgtag ttttgccagt gaggttgctc gatttctaca cccgaacgta cgccggcatt
     6481 tctcacccaa acccgcaggg tatgaatatc ttccgtacta aagccaaaac gggtttggat
     6541 cgcattcact tccagtaaat caaacagttc ttcggcatta aacgggcttt ctttcattgc
     6601 cagcagttgc aggaaactgt ggataatcgg atcaacctgc gtaaatgctt ggtcggataa
     6661 tgaaaaagga atatagcgag gatcccgatt accagtattg tagcgagaaa acaccgcatc
     6721 aatgtaagga gcgtatttat taatatccgg cgacatcaca ataatatctt tcggcagcaa
     6781 agtgctatct tgctcaaaca aattcaggag ctggttatgc aacacttcca cttcccgcat
     6841 tgggctgtgg cacgaatgaa tttggagtga attatcggcg gttaaatcgg taatagtggc
     6901 gtcattttct aagctgaaaa tctgtttttt gagtttattg aggttgctgt tttcctctgg
     6961 ttcgacaaag acatcaatgg tattcggctc aaattcgatt aattgagcta agaaatcccg
     7021 cccttgtttg ccccaaagat tcagtagttt atttctttgc tccgccaata agttatccag
     7081 atcgtcttgc gaaatcttgt ggcttaacgc caatttttgc cacacttttt cttccacact
     7141 gtcgccccaa tatttttcgc ttgggttaaa aaagaacagg tgaatatcgc aatgctcgct
     7201 gagtttctta aacaccgcta actgtaatgg cgagaaggac gaaatcccaa aaatgaaaat
     7261 gcgttttggc aatttggctt tttccgtttc agtaaggtca tctaacttag cgaagtaatt
     7321 ttgttgcaaa taggcacggt gggaggtagt aaaaacctgc tcatcagatt ccgattgcag
     7381 atcagataca atctgttgcc ataaatggct ttgccatttc atatcggctc gaattaagtc
     7441 aaaatcaatc ggcttagcgg cagttgcaat ttttatctca ttttcgatcg cttgtaagtt
     7501 gccttcttcc cagctaacca gccaatgggg gcgatacacc aaatattggt cgaacaattc
     7561 ggcaattttg gcagaaagtt gatagagctt aagctgatac tgttctttat taggttgatc
     7621 acctaaataa cgctttagcg gagcaaactc cgatagtgtc aagcaactcg gcaataaccg
     7681 cataatccgc caaaccaacg aactacgctc aaaaatattc tctttcggca gctccgggaa
     7741 caacagccga tactgttgcc ataaaaatgt agtagggtag agaaaatcgc aattccccat
     7801 cacaccgttt aattcagcaa tctgcatttg tagccactgt gccattccct gactttggat
     7861 caaaatggtt tccggctcaa acggatcttc aagtgggttt acctgctgga ttttcaccag
     7921 catctccgcc agtgatgaaa tttggttgga ttggtaaagt gtaaacataa taacgcctcg
     7981 ctaattgaat tattgtcctc caaagcgagg gacgattcaa tcattattca gctcaatttt
     8041 gcctaacgga aagcgaatgg ttaagcgatt gccagagcat tgaatctgat agcgaatctg
     8101 attttgagta atacttgatt cacaatctaa cccggctaat ttgagggcaa gctgattttc
     8161 taaaatcata accgcttgtt gctgttggaa atgttgattt agcctgtggt tttgtgccga
     8221 ttgccactgg ctgaaactca acaccacaat cgcaaatagc gtgatggcaa ccaaaagcga
     8281 caaagccgtt tctgctttat atttaatcat caaccgctcc catcaaatgt tcggaatatg
     8341 gcaaatatcg ccaagcggaa tacctcgcct caagctctgc cagtacctct tttttaaacg
     8401 ttaaatttcg ctcctctcgg gcattatcat aagaggaata aagcgtgccg tacatttttt
     8461 tgccggtaat atcaaacaga tagtcggtta tcacaatgcc gtaaaaatct tgcggtaagt
     8521 tttcattaat tgcgttttta gcaagcacaa ttttaggctc acgttcactg ctactaggta
     8581 attcagctaa agaatgaata gtataaatct cttggtttgg cacatttgat aaatctaaat
     8641 aatccgccaa acgggtaaag tgaatatatt tctctttagt cggtttttta tctttaaata
     8701 aactatggaa ttggcaggca aattgatact gtaaaaacct gaattgttta tcggataaat
     8761 cctcttggct gatctgctct ttttgaaatt tttggcatat ttgcgtttta tcttgtggat
     8821 ataacgctaa aaaattatat tgatcggaaa ggtatttctc tctataaaaa tgggtaatgt
     8881 tttgttgtcc aagccatttt tctttactta aaaataaaac cgacaaaatt gtagaaataa
     8941 gtattaatga aaccaataat gcactggcat ttttatattt attcactttt acctcgtatt
     9001 atttaagata aaaacataac gtactgccgt ataaatcaat cctttttgtt taggtgattc
     9061 tatttctaac tcaattttta ataagttatc ttcctgtctc caactaaaat ccagtttctt
     9121 agttttgaaa ttatttgctt cggtaaattt actccaattt ccatcgcaat tttccaataa
     9181 tgtttggcat tgttctttca cacaattatc taattgcgat gaaaaagtat aaatttcatt
     9241 attttctact ttaaagccaa caatttcttt taacacctct ctggtattat tcactgttcc
     9301 taacttacaa gcggccgttt ttgtcttacg ttttcccaaa cagccgtctt ggtttaaatc
     9361 gtaaaatcca ataatgcaat ttttatgcac aatattcagg cttttccctg cttgctgaaa
     9421 taaatcaaaa ttactgtctg ttctgtgaga gccttgatag ccaatgtgtt gaaaatgctg
     9481 ttggaagtag tttagtaact ggtgagcttc tgcttgcaag tagagctgtt ctttttgttt
     9541 ggtttgtgtg taatagaaat cggcataact tgaagcaaca agcaataata aaaacgcccc
     9601 taatgcacta gccattaata cttcaattag gctaaaagca gataaactaa tctttgcagg
     9661 tgctacgttt attttttgct gccacataaa cccgtcctaa ttgttcaaat tgcaaaattt
     9721 cattttcttt atttaaactg agcttaatac attttgattc taattgacca cttaccccat
     9781 caacattaat aaaggatttc ggatataaac ttttattttt taatacaatc tgctgatgag
     9841 tcggcgtata aagatgatat tcttttccta aatcacaaaa atgtttattc aaacaatcac
     9901 aaataatttg tttgctagta ttgattggct taattaccgc aatcatacac cactgattat
     9961 tccgctgaga aagggttaag gtataattac gtttggtata acgtgcttta gtttggattt
    10021 gctgaataaa agattggata ttttcaattt cactatgaag tgcaattcga tcttgcagtg
    10081 taaaaataac cggcgagata aaataaacag cgatcaccat aatagtgata gtggttagta
    10141 tttcaattaa tgtgatggct ttatacataa aaataccctc attgatagag ggtatcttag
    10201 aatttaagca tcatttgtaa actattaaat attgattttt cgatgcagat cgcaaaatta
    10261 aatcacttct acttaacttg ctctaaatat tcaaataaac gtcctttatt ttctttcgtg
    10321 cgtttttcaa ttttttccac ttctttttgt gtggcttcaa gattgagagc ttgtcctact
    10381 tggataattc cggccatatt catggttcgg tgtggcggac aatagtaaac ataaacacct
    10441 tcgtgagtaa gggtaatagt aaattcctca tcttctttag ataagaactt ctccgcacct
    10501 tccggaatcg agcggctttc aacccaatgc cctttatgag acggaataaa tgttaccgta
    10561 tcgcccacct ctgcttttac ataaccgggc tcaaatacca ttgatccttc cgcattgtta
    10621 tctaacattt tgatttcgtg atgggctgaa aatactgagg ctgaacaaaa tgccaatgtt
    10681 acacaagcta attgattcca cttaagtttc gctaacatat atttattgct cctaaattta
    10741 tgaggcacaa aatgagttca attcattata ccttaggtta ttccagctca acaccataaa
    10801 aattatgagc aataaacggg ctgacaacat agccttttac ctctttcctt accggaaaat
    10861 acaccgttga atgtgcaatc ggtaaccaag gtgcttgctc attaaagatg aatttcgctt
    10921 ttttatataa atctgaccgc ttgttttgat cactgatagt cgcagcctca ttaattaaac
    10981 ggtcaaactc tggattacaa aaccctgcat agtttgagcc ttgttctacc gcattacaac
    11041 tgagtagggc attgaggaaa ttatctggat cgccgttatc tccggtccaa ccaatcattc
    11101 cggtagcgtg ctcgccgttt cccattcgtt taagatattc tccccattca tagctcacta
    11161 tctttgcctc tacccctatt gctttccaat cctcttgaat catttcagcc attctacggg
    11221 catttggatt gtaagggcga gaaacaggca ttgcccaaat ttctgtttca aagccatttt
    11281 ctaatccggc ctctttaagc aagctcttcg ccttttccgg attaaattca taaccggcta
    11341 cttcgttgtt atatccccaa atcgtagaag gcattgggtt tttagctggc gttgcagagc
    11401 cttggtaaac ggaagttaaa atttcagttt tattcaccgc ataattcaat gcttgacgaa
    11461 ctttagggtt atcaaaaggg ggcttacgag tattccgata tttaggcctg acttttcaaa
    11521 taattgaata tctttattcg cttttaaagc aggcaaatct gacgggttcg gataaggcat
    11581 cgcttggcat tcgttcttct ccaatttagc taaacgtact gaggcatcgg gcgtaatact
    11641 aaatacaagg cgatctatct tcgcttttcc ttcccaatag tgttcaaatg ctgtaaatct
    11701 taccgcagcg tccttttggt aaccatagaa ctggaatgca cctgtgccta taggttctaa
    11761 atctaatttt tccggcgtac ccgctatttg catattccgc agaaaagatc gccataaaat
    11821 ccatagctaa attagcaaga aagggggcat ttggttcaga taattcgatt tttaccgtgt
    11881 aatcatctac ttttgttaca tttttgatga tgttttgcat attcattccg tagaaaaact
    11941 catagttacc gccgggcaat ttgtgatatg gcttcgtctt atcaaactga cggttaatcg
    12001 aaaaaataac atcatcagca ttaaaataac gactaggggt aaaactttta ttactctgga
    12061 actgcacatt ctgacacaag aaaaaagtat aaatttttcc gtcttcactg atttcccatt
    12121 cgctctgcca gagaaggagt gaggttagtt gtaccttttt caaaatcaat caggcgatta
    12181 tagattgctt gcccgacatc aagagtagct ccatctgttg caatctgagg attcaatgct
    12241 gccggagatg attccaaaca gtaagtaaaa gatttaggtg ctgcgaaagc agaacctgta
    12301 aatgctaaag aaataagtgc aagttgtgtc agcttgttca ttgtcatatc cttaaatgtt
    12361 aagcaataaa aaaccctttc aaaagttgcc ttccgaaagg gttagaattg ggtttgcgat
    12421 tttggattgc gtttgccatt tcaggttttc ggaagatatc ttccgaacac gccaaatgcc
    12481 cgaaagatta atcggtcaaa tggtagtgat gatgtttaac gcaatagctc ataatattct
    12541 ccaaaattga atgtttagga atttacgctt tttaaaaatc aattgcaaac gttttcttta
    12601 aaattttgta aaaaattcaa catttttaac cgcttgtatc gtacctagga gcaaatggcg
    12661 atttgctcct aggtttccaa gcaataggct attttttctt cgcatattta agtgagtcaa
    12721 tcgctaccgc caagataatg atgccacctt taataatata ttgccagtaa gggtttactc
    12781 caatataggt taagccgtag ttgataacgg tgaagattaa cacccctgtt actacaccga
    12841 tgatagtacc cacaccaccg gcaaaggata ccccacctac cacacaagca gcgattgcgt
    12901 ccatttcata cataaagccc aagttgttag tcgcacttcc gatacgtcct gcttctaaca
    12961 taccgccgaa ggcatagaag atgcctgaaa gcatataaat acccatcagg ttaagcggta
    13021 cgtttacacc ggaaactcgt gctgcttcag ggttgccgcc aatagcaaat acgtttttac
    13081 cgaagcgggt tttattccat aacgtccaca taataatggt agcaatcacg gcataaatag
    13141 tgatgtagga tagcctaaag tcgccaaagc ggaagaagcc ttgtgcaaag gttgagaaat
    13201 catcgctaaa gcctgcaatt ggcgatgagc ctactgcatc gtagtaaagc gagttgatac
    13261 catacacaat aatcattgtt cccatcgtgg caataaacgg tgttacgttg aggtaagcca
    13321 ccacaaagcc gttaaataaa ccaatgacag caccgatggc acacacggtt aatacaacca
    13381 caggaatcgg gatttccgag aggtttggga ataccttgtt catattttcc attgattgca
    13441 acaaggttgc cgagattacc gcagcaagcc ctacctgacg accggcagat aagtcggttc
    13501 cttgtgtgac aatcaagcct gcaaccccta atgcaataat cagacgcaca gaagattgcg
    13561 ataaaatatt actgaagtta cgcaagctca agaaacttgg atcttgcaca ataatcacaa
    13621 ttaataagat taaaagtaca aagtaaatcg cattttgttt aaaaaaatcg agcgttttat
    13681 tttgttgtaa g
//
LOCUS       DANKBV010000058        13488 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.058, whole genome shotgun sequence.
ACCESSION   DANKBV010000058 DANKBV010000000
VERSION     DANKBV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.058"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(174..563)
                     /locus_tag="PWY43_001383"
     CDS             complement(174..563)
                     /locus_tag="PWY43_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDL2136258.1"
                     /translation="MNISQAAEKTGLSTKQIRDYEKAGLLPVPARTQSGYRFYNEADL
                     DRLFFIGNARKVGFSLVQIAELLKLNDNPNRTSREVKQLTEQHIAELEQKINDLNEML
                     KLLRSWSRTCCGNNSPECSILNGLRDH"
     gene            637..837
                     /locus_tag="PWY43_001384"
     CDS             637..837
                     /locus_tag="PWY43_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2136259.1"
                     /translation="MAIKLQLDGLHCGNCVKSVEKALNDVSGVTQAIVTLENQTAVVE
                     GEVLAEDLIATVEDIGFGAKLA"
     gene            837..2981
                     /gene="cadA"
                     /locus_tag="PWY43_001385"
     CDS             837..2981
                     /gene="cadA"
                     /locus_tag="PWY43_001385"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2136260.1"
                     /translation="MTEQQLLIDGMHCAACVRRVEKALLKVENVSFASVNLADQTAFV
                     QGNANSEALIAAVTKIGFGAEILESEEERRAKLQAQTKRVLSHKRTQFIVALVAGFAL
                     MVFGFYKGMQITENNRSLWLVWSLITLATMYFSGREFFSGAYNALKNKSANMDVLVAI
                     STGTAWLYSFWLTLFPQPNAHVYFEASVMIIGFINLGKYLELKAKQRSSLALEKLLDL
                     APKQAVIFEENIAKTIPAKGIKPEMRVQALTGDRLAVDGILESGSIWVDESMLTGEAL
                     PIEKKVGDKVRAGTLVQDGSGVYIAEQVGSQTALARVIHAVRHAQSSKPPLAQFVDKV
                     AAVFVPVVVSIALFAALIWLLVGKDFAFALSIFTTVLIIACPCALGLAIPLSTIAGVA
                     RAAEFGVLVRNIQALQAGSEIDTLVFDKTGTLTTGQMEVTEVITFNNFDRNDLITLTK
                     SLEQHASHPIAKAIVKFAENYPSLDVNEIQVEKGLGIRGKVGEDRVKIGNASFVNFSE
                     KTTACTATLIYIAVNDEIIGRISVQDQLRPEAKETIQRFQAEGYQCLMLTGDRQETAD
                     YFAKELGLNGVIAEVLPEQKAEKIRALQAEGKKVAMIGDGINDAPALAQANVGIAMHN
                     GSDIAVETADLSLMKSGLALVSQILPFSKRVLKNMKQSLLGAFIYNVVAIPVAAGMLY
                     PFTGWLLNPMIAAIAMTLSSITVVLNSQRLLK"
     gene            complement(3034..3819)
                     /gene="truA"
                     /locus_tag="PWY43_001386"
     CDS             complement(3034..3819)
                     /gene="truA"
                     /locus_tag="PWY43_001386"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2136261.1"
                     /translation="MKIALGIEYDGSRYFGWQRQEAVESVQQQLEQALSVVANSPVEV
                     FCAGRTDAGVHGTGQVVHFETQANRPLQSWCFGTNAHLPDDIAVKWAVAVNEDFHARF
                     SATARRYRYIIFNQKLRSAILPKGVTHFHFPLDERKMHQAGQYLLGENDFSSFRAAKC
                     QSNTPWRNIHHLNVSRQGNYVIVDIQANAFVHHMVRNIVGTLLEIGQGKQPVEWAKWV
                     LEQRDREKAAPTAKAEGLYLVEVHYPEHFGIPQTPLGPLFLAD"
     gene            complement(3892..4581)
                     /gene="folE"
                     /locus_tag="PWY43_001387"
     CDS             complement(3892..4581)
                     /gene="folE"
                     /locus_tag="PWY43_001387"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDL2136262.1"
                     /translation="MTTISPELTITENTLSPEAQKVRQALLAKGIETPTVAHTKDKAS
                     RRAEIQQHMQAVLELLGLDLNDDSLEETPHRLAKMYVDEIFSGLDYATFPKITNIENR
                     MKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQV
                     QERFTEQILTAFQTILETDDVAVYVKATHFCVKCRGVKDTNSYTVTSAFGGVFLEDRE
                     TRKEFLGLLNK"
     gene            4682..5389
                     /locus_tag="PWY43_001388"
     CDS             4682..5389
                     /locus_tag="PWY43_001388"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HDL2136263.1"
                     /translation="MQNIQYLFNADLIPEHQAEFVQSLLTCKNFDNSDRLLGFSKGRG
                     ATWFLDTSAELGINVVLRHYYRGGLFGKIIKDSYFFTRFEATRAYKEFFLLQQMLAWG
                     LPVPRPIAIKVERNFCCYRADIMLEKLENTQDLSKILQSHSLSNEQYEQIGKLIRSLH
                     NHQIHHSDLNIHNILLGNDGKFWLIDFDKCGIEPGENWKQNNLDRLLRSFKKEQGRLN
                     IFFSEENWQAVLRGYTL"
     gene            complement(5444..6541)
                     /gene="apbC"
                     /locus_tag="PWY43_001389"
     CDS             complement(5444..6541)
                     /gene="apbC"
                     /locus_tag="PWY43_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDL2136264.1"
                     /translation="MNQVNEQQLVEIKAILQQFSHPTLQKDLIALNAFKKAELGGDIL
                     RIEITMPFAWNSGFETLKAETEAKLKQVTGASGVKWVLNYQIATLKRANKHPAVNGVK
                     NIIAVTSGKGGVGKSTTSVNLALALRAQGARVGILDADIYGPSIPHMLGAKDQRPTSP
                     DNKHITPITAHGLQSNSIGYLMEEDNATIWRGPMASSALSQLLNETWWNELDYLVIDM
                     PPGTGDIQLTLSQQIPVTGAVVVTTPQDIALLDAIKGISMFEKVSVPVLGVIENMSMH
                     ICSNCGHHEAIFGTGGAEKIAQKYGTKVLAQLPLHIRLREDLDSGVPTVVAAPEHEIS
                     QAYLALAEKVASELYWQGSVIPSEIMIREVK"
     gene            complement(6538..8091)
                     /locus_tag="PWY43_001390"
     CDS             complement(6538..8091)
                     /locus_tag="PWY43_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2136265.1"
                     /translation="MLKSLQAVTFVKKIAKVTACILLITSCDQLICPQSIQSVWVENR
                     QSDTLLKRAIYSAVFQLDPHKIKASADAAPLRDLLVGLMAYNRKGDVVPAIAQNWFTE
                     DEKEWLFILDEKAVWSNGQPVTAQDFVASWRRLIDPQNHSVLSKYLIYMGIENAKAIE
                     EQIKSVEELGVEALNAHTLRIRLNHPNHQLPEMLAHSTLLPTYQGIAPTIDNFVSNAS
                     YRLEKLDAQKMVLKARTQEIPFQTIEYDLITTIQNCSAFDIVENPLLNKKTNIVKFPC
                     LCSYYYEFNFDDPLLAKREIREAIRTMVASANIGQEYGVPSQSVIPHSLVKDREKRWN
                     PVVVEQLLTKAGISPYNPLKLTLLYDENSININIANQVTRALSQSELFNITPKMVDWQ
                     TLNKVRGQKDYQIARAGWCADYPDPIVFLQKFHSMSPDNHSGYKSEIVDQKLNRLQTE
                     TLSAEARESLIQEISEQLYSDVAVLPLFQYHHRVGIVSSLLGIDLNNDSEVIYSKDLK
                     RNLANKDNQ"
     gene            complement(8117..8767)
                     /locus_tag="PWY43_001391"
     CDS             complement(8117..8767)
                     /locus_tag="PWY43_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HDL2136266.1"
                     /translation="MDIIINFAIYLQFFIGLFAIVNPFGSLPIFFSMTAHQYEGERNR
                     TSLITAVSIGVILLVSLYFGNLILNAFSISLSSFRVAGGILIVSIAMTMISGKLGEHK
                     QNKEEKNEDITEYENIGVVPLAMPIMAGPGAIGSTIVWGTRYHTWEDYVGFSIAIIVF
                     ALICYILFRYSAPLVKKLGKTGSNVVTRIMGLILMALGIEIIVAGITTLFPVLLVK"
     gene            complement(8889..10760)
                     /locus_tag="PWY43_001392"
     CDS             complement(8889..10760)
                     /locus_tag="PWY43_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HDL2136267.1"
                     /translation="MIEKMHERTNGPVFKIIFALVSISFVIGGIGTGLISQDNSVAKV
                     NGEEISQQLFNNTLNREQNRLNVELGSRFWDLMDSPEYAAQFNQSVLNGLIDEELLRQ
                     YAKELKLGISADQIKSEIVNSQMFQQDGKFSNELYQQTLRHNNLSADGYAAIVQEGML
                     QSQLQEGIINSDFNVPAQQALLAKLLLQQREVRLAEFSVAKEMENQTASEQELRAYYE
                     ANKTKLLAPEKLAVEYVTLSPKDVESKVEITNEQIQTYYDRNKADYVTKGEAHLAHIQ
                     VASEAEAQAIEQALKNGEDFATLAKNKSSDTLSANNGGDLGWAKAGTFPKAFEDAVAN
                     LTAGQMSAAVKVDNAYHIIKVLERKPEQVIELAQVKDKIAQTIRQELVLTEYSTVARE
                     MANKAFENNSSLEEVAKAGNVELQKTDLFTRENVPTALQNEKVLKVLFEGELRQNKQN
                     SDALDVGNDANPQTLFVRVSDYDAERPQTFDEAKAAVENFVKAEKAEKALLAKVEENV
                     KALNEGKSVDVKFNAPQTLVYMQAEVENPVLAGTIFAMKKAQDKTTYQMARNQKGDVV
                     IVALDNITEGNAEQFKTLAPQFEQANRLILRNEFLQSLRAKASIEVNEDFMQQRSSH"
     gene            10942..11211
                     /gene="rpsO"
                     /locus_tag="PWY43_001393"
     CDS             10942..11211
                     /gene="rpsO"
                     /locus_tag="PWY43_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL2136268.1"
                     /translation="MSLSVEAKAKIVAEFGRDAKDTGSSEVQIALLTAQINHLQAHFA
                     EHKKDHHGRRGLLRMVSRRRKLLDYLKRTDLAKYSETIARLGLRR"
     gene            11283..12152
                     /locus_tag="PWY43_001394"
     CDS             11283..12152
                     /locus_tag="PWY43_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDL2136269.1"
                     /translation="MAFASLFTLLDDIAAVLDDVSIMTKVAAKKTAGVIGDDLALNAN
                     QVSGKNVVPERELPIVWAVAKGSLINKVILIPLALLLSVFLPQAIIPLLMIGGAYLCF
                     EGVEKLLHRLLHKNNQAHSDETETLSEKDKIKGAIRTDFILSAEIIIIALGVLQDSST
                     LTQILSLSAIGVGITLFVYGLVGLIVKLDDIGLWLMQKKSTFSQAVGKGLLFAMPWFM
                     KGLSVIGTIAMFLVGGGIFTHNLPMIHHFVEQFGISPHFSTLVDFAVGIVVGIIACLI
                     ILPLMKLFSKKAH"
     gene            complement(12242..13486)
                     /locus_tag="PWY43_001395"
     CDS             complement(12242..13486)
                     /locus_tag="PWY43_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HDL2136270.1"
                     /translation="MLLLLVGLISGPYLAGNQGYVRIETATTVIEMSIVMLVVFFVLA
                     MAVVYLLEWIVTKICRMSNGAYNWFSTRKHKKAQQETLEGLMRMSEGNYSKAEKLFSK
                     NAKHADEPVLNLIKAAEAAQQNGDDLAANKYLIKATEIAGPNNVAVELVRTRILLQQG
                     KLPAARTAIDSLLELAPHSDEVLRLAIQIYKESRAYVALDRLLVEIGQRNFLTAEEYA
                     ELEYFVDDGLQDERLQEEGQEGLLSWWENQPNRRRKSVYSRVGVIKRLIDSDDHQSAQ
                     EIALETLKKFEDEQLQGLFVQLTRLQIDSDSKLLKVLAKRADKANVKYTDDYARALGY
                     IYTRDGQYQKAKSQFELLLAHDECVADDRIMALHVAEQMGDTLMANRIREENLKQVNM
                     ATQSKVNEPLALPQTATESNSL"
BASE COUNT         3870 a         2843 c         2550 g         4225 t
ORIGIN      
        1 ttagtgaaat atgaccgctc gtatagctat ttatagcttc cactctccgc caatttttta
       61 gaaacgtcat cattgagtcc atcttattgt gaagcaaata aggttcgttt ttgattattt
      121 gtcataattt ttacctatag actaaagtca ttctatttta ctgcattttt agcttaatga
      181 tccctcaacc cattcaaaat tgaacactca ggactgttgt tgccacagca agtacggctc
      241 cagcttctga gtaatttaag catttcgttt aagtcattta ttttttgttc cagctctgca
      301 atatgttgct cggtgagctg ttttacctct cggctggtgc ggtttgggtt gtcgtttagt
      361 ttcaataatt cggcaatttg cacaagtgaa aagccgactt ttcttgcatt accaatgaag
      421 aacaatcggt ctaaatccgc ttcattataa aaacgataac ccgattgtgt gcgagctgga
      481 accgggagca gtcctgcttt ctcatagtct cgaatttgtt tggtagatag ccctgttttt
      541 tcggcggctt ggctaatgtt catattccct cttgacttta acctaaggtt aaaccttatt
      601 atatcctcga ataaccactc aacataggag aaaataatgg caatcaaatt acaattagac
      661 ggattgcatt gtggtaactg cgtgaaaagt gtcgaaaagg ctttaaatga cgtttcggga
      721 gtaacgcaag ctatcgtaac cttagaaaat caaacagcgg tagttgaagg cgaagttttg
      781 gctgaagatt taattgcaac agttgaagat attggttttg gagcgaaatt agcataatga
      841 ccgaacaaca gcttttaatt gatggaatgc actgtgctgc ttgtgttcgc cgagttgaga
      901 aagcattgct taaagtagaa aacgtcagtt ttgcctctgt taatcttgct gatcaaaccg
      961 cttttgtgca aggaaatgct aattcagaag cattaattgc cgctgttact aaaattggtt
     1021 ttggggcaga gatattagaa agcgaggaag agcgtagagc caagctgcaa gctcaaacta
     1081 aacgtgtgct aagccataag cgtacacaat ttatcgtggc tcttgttgca ggctttgcgt
     1141 tgatggtgtt tggcttttat aagggtatgc agattaccga aaacaatcgc tcgctttggc
     1201 ttgtttggtc tctcatcacg cttgctacaa tgtatttttc gggcagagaa tttttttcag
     1261 gggcttataa cgcccttaaa aataaatctg ccaatatgga cgtgttagtc gccataagca
     1321 caggcacggc ttggctttat tcattttggc ttacgctgtt tccacagcca aatgctcacg
     1381 tctattttga agcgagtgtg atgattatcg gctttatcaa tttaggcaaa taccttgaac
     1441 ttaaagccaa gcaacgctct tcccttgcat tggaaaaatt gctcgatctc gccccgaaac
     1501 aagcggtcat ttttgaagaa aatattgcaa aaactattcc agccaaaggc attaaacctg
     1561 aaatgcgagt gcaagcctta acgggtgatc gcctagcggt tgatggcatt ttagaaagtg
     1621 gctcaatttg ggttgatgaa tcaatgctga caggcgaagc cttgccgatt gagaaaaaag
     1681 taggcgataa agtccgagct ggcacgctgg tgcaagatgg ttcaggggtt tatattgccg
     1741 agcaagttgg ttctcaaact gctttagctc gtgtgattca tgccgttcgc catgcccaaa
     1801 gtagcaaacc accacttgct caatttgttg ataaagtcgc cgctgtattt gtgcctgtag
     1861 ttgtttctat tgcattattt gctgctttaa tttggcttct tgtcgggaaa gatttcgcct
     1921 ttgcactttc catttttacc accgtgctga ttattgcttg cccttgtgcc ttagggttag
     1981 ccattccgct ttcaaccatt gcaggggtag ctcgtgcggc tgaattcggt gtattagtga
     2041 gaaatattca agccttacaa gcagggagtg aaattgatac tctagttttc gataaaacag
     2101 gcacgctcac cacagggcaa atggaggtta cggaagttat taccttcaat aattttgata
     2161 gaaatgattt gattactctt accaaaagtt tagagcagca cgcctctcac cccattgcga
     2221 aagcgattgt aaaatttgcg gaaaattatc cctctcttga tgtcaatgaa attcaagtgg
     2281 agaaaggatt gggcattcga ggcaaggtgg gagaagatag agttaaaatc ggcaatgcct
     2341 catttgtaaa tttttcggaa aaaacaaccg cttgtactgc cacacttatc tatattgcag
     2401 ttaatgatga aataattggc agaatttcgg tgcaagacca acttcgccct gaagcgaaag
     2461 aaacaattca acggttccaa gctgaaggtt atcaatgcct aatgcttaca ggcgatcgcc
     2521 aagaaaccgc tgattatttt gccaaagagc tgggcttaaa tggcgtgatt gctgaagtct
     2581 tacccgaaca aaaagcggag aaaattcgag cgttgcaagc agaaggaaaa aaagtggcga
     2641 tgattggcga tggcattaat gatgccccag cccttgctca agcgaatgtg ggaattgcaa
     2701 tgcataatgg atcagatatt gcggtggaaa ccgccgatct ttctctgatg aaaagcggat
     2761 tagcacttgt aagccaaatt ttacccttct caaaacgagt gctaaaaaat atgaaacaga
     2821 gcctgctagg tgcattcatc tacaatgtgg ttgcgattcc tgtggcagca ggaatgcttt
     2881 atccgtttac tggttggctg ctcaatccaa tgattgctgc gattgcgatg acattgtcct
     2941 ccattacagt ggtgctgaat agccaaagac tgttgaaata attcaagcgg ttggatttgc
     3001 aaaaaaattt acaaactcaa ccgcttgtct taattaatcc gccaaaaaca gcggcccaag
     3061 aggtgtttgg ggaatgccga aatgttcagg gtaatgcacc tcaaccaaat atagcccctc
     3121 agctttcgct gtaggggcag ctttttcccg atcacgttgt tccagcaccc attttgccca
     3181 ttccacaggc tgcttgccct gcccgatttc gagcaacgtt cctacaatat tgcgtaccat
     3241 atgatgcaca aaggcgttgg cttgaatatc aacaatcaca taattgcctt gccgagacac
     3301 attcaaatga tgaatattcc gccacggcgt attagattga catttagcag cacggaaaga
     3361 ggaaaagtca ttctcgccca gcaaatactg ccctgcttgg tgcattttgc gttcatcaag
     3421 gggaaagtga aagtgggtaa cacctttagg taaaattgcc gaccgcagtt tttggttaaa
     3481 aataatatag cggtaacgac gggcggtggc actaaaacgg gcgtgaaaat cctcattcac
     3541 cgctaccgcc catttcaccg caatatcatc gggtaaatga gcgttagtgc cgaaacacca
     3601 gctttgtaac gggcggtttg cttgcgtttc aaaatgcacg acctgcccag tgccgtgcac
     3661 accggcatcg gttctgccgg cacaaaacac ctccaccggc gaattggcaa caaccgacaa
     3721 cgcctgctct agctgttgtt gcacgctctc taccgcttct tgtcgttgcc agccaaaata
     3781 acggctgccg tcatattcaa tacctaatgc tattttcatt tcaacctcaa gcacctgatg
     3841 caaaatataa gggcgaaaaa ttttcgcccc cacagtaatt gatatttgca attatttatt
     3901 caataaacct aaaaattctt tacgggtttc tcgatcttct aagaaaacac cgccaaatgc
     3961 agaagttacc gtatagctat tggtgtcttt tacaccacgg catttcacac aaaaatgggt
     4021 cgctttcaca taaactgcga catcgtccgt ttctaaaatg gtctgaaatg cagttaaaat
     4081 ctgttcggta aaacgctctt gcacctgcgg acgttgggca aaaaactgca cgacacggtt
     4141 gattttcgag agtccaatca cccatttttt cgggtaatag gctaccgcca ctttaccgtc
     4201 aatagtgaca aaatgatgct cacaggtaga agttaaagta atatcgtcca ccagcaccat
     4261 ttcactgact ttcattcggt tttcaatatt ggtaattttc gggaaggtag cataatcaag
     4321 cccgctgaaa atttcatcga catacatttt cgccaagcga tgaggcgttt cttccaaact
     4381 gtcatcgttc aaatctaaac cgagcaattc taaaactgcc tgcatatgct gctggatttc
     4441 tgcacggcgg ctggctttat ctttagtatg agcaacagtc ggagtttcaa tgccttttgc
     4501 caacaaggct tggcgaactt tttgagcctc cggcgaaagc gtgttttctg tgatagttaa
     4561 ttcgggcgag atcgttgtca ttggttttcc tctcaaatgt aagcggaata ttctaacaaa
     4621 ttgacatcaa aaaaaatagc tctaaaatga gctatttcag ccataaacac cctctttatt
     4681 tatgcaaaat atacagtatc tctttaatgc cgacctaatt cccgaacatc aagctgaatt
     4741 tgtgcagtct ctcttaactt gcaaaaattt tgacaattcc gaccgcttgc taggtttctc
     4801 aaaagggcga ggtgcgactt ggtttttaga tacctctgcc gaactaggta tcaatgtagt
     4861 gcttcgccat tattatcgtg gcggcttgtt cggtaaaatc atcaaagaca gttacttttt
     4921 tacccgtttt gaagccaccc gagcttataa agaatttttc ttactgcaac aaatgttagc
     4981 ttggggctta cctgtgcctc gcccaatagc gatcaaagta gaaagaaatt tttgttgcta
     5041 tcgtgccgac attatgctcg aaaagctaga aaacacacaa gatttaagca aaatcttgca
     5101 atcccattct ttatcgaacg aacaatatga gcagataggg aaactgattc gcagtctgca
     5161 taaccatcaa attcaccatt ccgacttaaa tatccataat attttgcttg gtaatgatgg
     5221 caaattttgg ctgattgatt ttgataaatg cggcattgag ccgggcgaaa attggaagca
     5281 aaataaccta gaccgcctgt tacgctcgtt taagaaagaa caagggcgat taaatatttt
     5341 cttcagcgaa gaaaattggc aggctgtttt gagaggatat actctctaag aaacaataat
     5401 ggcacaagct tagaaacttg tgccatcaaa tcatcaacat taactacttc acttcacgaa
     5461 tcataatttc agacggaatc acactgcctt gccagtaaag ttcagaagct actttttccg
     5521 ctaatgcgag gtaggcttgg ctgatttcgt gttccggagc tgccaccacc gttggcacac
     5581 cgctatctaa atcttcacgc agacgaatat gtagtggtaa ttgtgctaac actttagtgc
     5641 catatttttg ggcgattttt tccgctccgc cggtgccgaa aattgcctca tggtgtccac
     5701 agtttgagca gatatgcatt gacatatttt caatcacccc aagcactggt actgagactt
     5761 tctcaaacat tgagatccct ttaatcgcat ctaataaggc aatatcttgt ggggttgtta
     5821 ctaccaccgc accggtcacc ggaatttgct gtgaaagcgt aagctgaata tcacccgtac
     5881 ccggtggcat atcaatgacc aagtaatcca attcattcca ccaagtttca tttaaaagct
     5941 gacttaacgc actgcttgcc atcgggccac gccaaatggt ggcgttatct tcttccatta
     6001 aatagccgat agaattagat tgcaagccgt gagcggtaat cggcgtgatg tgcttgttat
     6061 caggagaagt tggacgttgg tcttttgctc ccaacatatg cggaatagat gggccgtaaa
     6121 tatccgcatc taaaatcccc actcttgccc cttgtgctcg taaggctaaa gcaagattaa
     6181 ctgaggtcgt tgatttccct accccacctt ttcctgaagt gaccgcaatg atatttttta
     6241 ccccattgac tgccgggtgt ttattcgccc gttttaaggt cgcaatttgg taatttaata
     6301 cccattttac accgcttgca cctgttactt gctttaattt agcctctgtt tcggctttta
     6361 aggtttcaaa accactgttc cacgcaaatg gcatcgtgat ttcaattcta agaatatcgc
     6421 cccctaattc tgctttttta aacgcattaa gggcaattaa atctttctgt aaagtcgggt
     6481 gggaaaattg ttgcaagatt gctttaattt caacaagttg ctgttcattt acttggttca
     6541 ttggttatcc ttgtttgcta aattgcgttt taaatctttg ctataaatta cttcgctatc
     6601 gttattcaaa tcaataccca aaagggagga aacaatccca acccgatgat ggtattgaaa
     6661 taatggcaat acggcaacat cgctataaag ctgctcgcta atttcttgaa taagtgactc
     6721 tctcgcttct gcactaagcg tttctgtttg taaacgattt aatttttgat cgactatctc
     6781 acttttatag ccggaatgat tatccgggct cattgaatga aacttctgca aaaatacaat
     6841 cggatcagga taatctgcac accagcctgc tcgtgctatc tgataatctt tttgtcccct
     6901 tactttattt aaagtctgcc aatcaaccat tttaggtgta atattaaata attctgattg
     6961 agaaagggct cgagtaactt ggttagcaat attaatatta atgctattct catcatataa
     7021 caaagtcagc tttaaaggat tataagggct aattcctgct tttgttaata gctgctcaac
     7081 cacaacagga ttccaacgct tttctctatc ttttactaag ctatgtggaa ttacagactg
     7141 actcggaacc ccatattctt gcccaatatt tgctgaagcc accattgtcc ttattgcttc
     7201 acgaatctct cgtttagcta ataatggatc atcaaaattg aactcatagt aataagagca
     7261 caaacacgga aatttcacga tattcgtttt tttatttaat aaaggattct cgacaatatc
     7321 aaaagcagag cagttttgga tagttgtaat taaatcatac tctatagttt gaaagggaat
     7381 ttcttgggtt cgagccttta acaccatttt ttgagcatct agtttctcga gacggtaaga
     7441 cgcattgctc acgaagttat ctatggttgg agcaatccct tgataagtag gtaataatgt
     7501 ggaatgtgcc agcatttcag gcaattggtg atttgggtga tttagccgaa ttctcaatgt
     7561 atgcgcattt aatgcctcta caccgagttc ttcaactgac tttatttgct cttcaatcgc
     7621 ttttgcattt tctataccca tatagatgag atatttagac aatacagaat gattttgagg
     7681 atctattaaa cgtcgccagc ttgctacaaa atcttgtgca gtcacaggtt ggccattaga
     7741 ccacacagct ttctcatcta aaataaacaa ccactctttt tcatcttcag taaaccaatt
     7801 ctgagcaatg gccggaacga catcaccttt tctgttgtag gccattaaac cgactaatag
     7861 atctcttaaa ggagcagcat ctgcagaagc tttaatttta tgtgggtcga gttgaaatac
     7921 agcagaataa attgcacgtt tgagtaaagt atcactctgc cggttttcta cccagacaga
     7981 ttgaatggat tgagggcaaa ttaattgatc acaagatgta ataagcagaa tgcaagcggt
     8041 aacttttgca atttttttta caaatgtaac cgcttgtaag gatttcaaca taaactcccc
     8101 aaaggttaat taagatttat tttacaagaa gcacaggaaa taaagttgta attcctgcca
     8161 caataatttc aattcccagc gccattaaaa tcagccccat gatacgagta acgacattag
     8221 atcctgtttt gcctaatttt ttcaccaaag gggctgaata gcggaataaa atataacaaa
     8281 ttaatgcaaa tacaataatc gcaatgctaa agccaacata gtcttcccaa gtatggtagc
     8341 gggttcccca cacaatggtt gagccaattg cccctggtcc tgccataatc ggcattgcta
     8401 atggcacgac accgatattt tcgtattcag taatatcttc atttttctct tctttatttt
     8461 gtttgtgttc acctaattta ccgctaatca tcgtcatcgc aatgcttaca atcaaaatgc
     8521 caccagctac tctaaaagag ctaagtgaaa tgctaaacgc attgagaatc agattaccaa
     8581 aatagaggct gactaataaa attactccaa tagaaacagc ggtgattaag ctggtgcgat
     8641 ttctttcccc ctcatattgg tgagcggtca tactgaaaaa aataggcaag gaaccaaaag
     8701 gattgactat agcaaataga ccaatgaaga attggaggta gatggcaaaa ttaataatga
     8761 tatccacaaa aactttctca aacaacagtg tttcgggcgc tattatatcg gttaatcaag
     8821 gtagatacaa accaaaagca cccagcctaa aggacggttg ggtgcttttt gataaaatga
     8881 aaggcttgtt agtgtgacga acgttgctgc ataaaatctt catttacctc aattgaagct
     8941 tttgcacgta gcgattgtaa gaattcatta cgtaaaatta aacgattagc ttgttcaaat
     9001 tgtggcgcca atgtcttaaa ctgttcagca ttcccttccg taatgttatc taaggcaaca
     9061 ataactacat cgcccttttg attacgtgcc atttgataag tcgttttatc ttgcgccttt
     9121 ttcattgcga aaatcgttcc agccaatacc gggttttcta cttctgcttg catataaact
     9181 aaagtttgtg gcgcattaaa tttcacgtcc actgatttcc cttcattcaa ggcttttaca
     9241 ttttcttcta ctttcgccag caacgctttt tccgcttttt ctgccttcac aaaattttca
     9301 actgctgctt ttgcttcatc aaaagtttgt ggacgttctg catcataatc actcactctc
     9361 acaaacaatg tctgtggatt tgcatcattc ccaacatcta aagcatcaga attttgttta
     9421 ttttggcgta gctctccttc aaacaaaact tttaatactt tctcattttg taaagcagta
     9481 ggtacgtttt cacgggtaaa cagatccgtt ttttgcagtt ccacattacc ggctttcgca
     9541 acttcttcaa gtgaagaatt attctcaaag gctttatttg ccatttcgcg cgcaactgta
     9601 gaatactcag ttaacactaa ttcttggcga atagtttggg caattttatc tttcacttgt
     9661 gctaactcaa tcacttgctc aggtttacgc tccaatactt tgatgatatg gtaagcatta
     9721 tccactttca ccgctgcact catctggccg gcagttaaat ttgctacagc atcttcaaaa
     9781 gctttcggga aagtgccagc ttttgcccaa cctagatcac caccattatt tgctgataaa
     9841 gtatcgctag atttgttttt tgccaaagta gcaaaatctt ccccattttt gagcgcttgc
     9901 tcaatagcct gtgcttccgc ttcactagcg acttgaatat gagctaaatg ggcttcacct
     9961 ttagttacat aatcggcttt attgcgatca tagtaagttt gaatttgctc attggtaatc
    10021 tcaactttgc tttccacatc ttttggagaa agagtaacgt actcaaccgc taatttttca
    10081 ggtgctaata atttagtttt atttgcttcg taataggctc gcaattcttg ctcggaagcg
    10141 gtctgatttt ccatttcttt tgcaactgaa aattcggcta aacgcacttc acgttgttgc
    10201 aacagtaatt ttgctaacaa tgcctgctgt gccggtacat taaaatcact attgataatc
    10261 ccctcttgta gctgggattg caacatacct tcctgcacaa tcgctgcata gccatcggca
    10321 cttaaattat tgtggcgtaa tgtttgttgg tataactcgt tactgaattt accatcttgt
    10381 tgaaacatct ggctgtttac aatttcagat ttgatttgat cagcactaat acctagtttt
    10441 aactcttttg catactggcg cagaagctct tcatcaatta atccatttaa taccgattga
    10501 ttaaattgag cagcatactc cgggctatcc attaaatccc aaaagcgact ccccagttca
    10561 acatttaatc tattttgctc acgattaagc gtgttattaa ataattgctg actaatttct
    10621 tccccattta ctttcgctac agaattatct tgtgaaatca atcctgtccc aattccaccg
    10681 attacaaatg aaatagagac taatgcaaaa ataattttaa agaccggccc atttgtccgc
    10741 tcgtgcattt tttcaatcat ttaaaaattc ctaaattact caaaaattgt cgctgattat
    10801 actctttctc aaatgagaaa taaacttgca aatttttatt gaaatttaac cgctttctct
    10861 ttaaaattca cagtccctgt cgtagctgag ttagagatcg gctattgatg tattatcaac
    10921 ccttaacaaa aggaaattaa aatgtctcta agcgtagaag caaaagcaaa aatcgttgct
    10981 gaatttggtc gtgatgcaaa agacactggt tcttctgaag ttcaaattgc attattaact
    11041 gcacaaatca accacttaca agcacacttt gctgagcata aaaaagatca ccacggtcgt
    11101 cgcggtttat tacgtatggt ttcacgccgt cgtaaattat tagactactt aaaacgtact
    11161 gatcttgcta aatattcgga aactatcgca cgtttaggtt tacgtcgcta atttcaaata
    11221 ttagatatga gagccgagct gaaaagttcg gcttttttat aaattttatt cgaggaaaaa
    11281 atatggcttt tgcgtcatta tttacattat tagacgatat tgctgccgtg cttgatgatg
    11341 tatcgatcat gactaaagtg gctgctaaga aaacagcagg cgtaatcggt gatgatcttg
    11401 ccttaaatgc taatcaagtc agtggaaaga atgtggtgcc agaacgagaa ctaccgattg
    11461 tttgggctgt agcgaaaggc tcattaatca ataaagtgat cttgattcca ctagcgttgc
    11521 tgctatcggt ctttttaccg caggctatta tcccgctatt aatgataggc ggtgcatatt
    11581 tatgttttga aggcgtagag aaattactcc atcgtctatt acataaaaac aatcaagctc
    11641 attctgatga aaccgaaacc ctttctgaaa aagataaaat taaaggtgca attcgcaccg
    11701 attttattct atcagcagag attatcatta ttgcgttagg tgttttgcaa gactcttcaa
    11761 ccctaaccca aattttatct ctctccgcta tcggggtcgg aattaccctg tttgtttatg
    11821 gcttagttgg attaattgta aaactagatg atatcggttt atggttaatg cagaaaaaaa
    11881 gcacattctc acaagcggtt ggaaaaggct tattatttgc aatgccgtgg tttatgaaag
    11941 gactttctgt gattggtact attgcgatgt tcttagtcgg tggtggtatt tttacccata
    12001 atttaccaat gattcatcac ttcgtagaac aattcggtat ctctccccat ttttctactc
    12061 tggttgattt tgcagtgggt attgtggttg gtattattgc atgcttaatt attttacctt
    12121 tgatgaaatt attttctaaa aaggcgcact aatccccaaa agcgtagctt tggttaaagc
    12181 tagcttttta tctcttgcta taaaaaaata gcaggtttca agcctgctat ttttttaatt
    12241 attataatga gttagactca gtggctgttt gcggtaaggc taatggctca ttaactttag
    12301 actgagtagc catattcacc tgtttgagat tttcctcacg aatacggttt gccattaatg
    12361 tatcccccat ctgctctgcc acgtgcagtg ccataatacg atcatcagca acacattcat
    12421 catgagccaa taacaattca aactgagact tcgctttttg gtattgccca tctcgtgtat
    12481 aaatatagcc aagcgctcta gcataatcat cggtatattt aacattcgct ttatctgccc
    12541 gttttgctaa tacttttagt agcttactgt cagaatcaat ttgtaaacgg gttaattgca
    12601 caaataaacc ttgtaattgc tcatcttcaa atttcttcaa cgtttctaaa gcaatttctt
    12661 gtgcagattg atgatcgtca ctatcaatta aacgcttgat gacaccgaca cgactataca
    12721 ccgatttacg acgacgattt ggttggtttt cccaccaaga aagcaagcct tcctgccctt
    12781 cttcctgtaa acgctcatct tgtaaaccat catcaacgaa atactctaac tcagcatact
    12841 cttctgcagt taagaaatta cgttggccga tttcaactaa taatctatct aacgcaacat
    12901 aagctctgga ttctttataa atttgaatcg ctaagcgtaa tacctcatcg ctatgaggtg
    12961 caagctctag taggctatca atcgcagttc gggcagccgg taattttcct tgttgtaata
    13021 aaattcgggt tcgtaccaac tccactgcaa cattattcgg tccagctatt tcagttgcct
    13081 taattaaata tttatttgca gctaaatcat caccattttg ctgagcagct tccgccgctt
    13141 taattaaatt cagtacaggc tcatctgcat gttttgcatt tttactaaag agtttttccg
    13201 cttttgagta attcccttca ctcatacgca ttaaaccttc cagtgtttct tgctgagctt
    13261 ttttatgctt acgagttgag aaccagttat aagctccatt actcatacgg catattttag
    13321 tgacaatcca ttcaagtaaa taaactaccg ccattgccaa cacaaaaaaa accacaagca
    13381 ttacaatgct catttcaatc acggtagttg ctgtttcaat tctgacataa ccttgatttc
    13441 cggctaaata aggcccagag attagtccaa ctaataataa aagcatca
//
LOCUS       DANKBV010000059        12756 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.059, whole genome shotgun sequence.
ACCESSION   DANKBV010000059 DANKBV010000000
VERSION     DANKBV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12756
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.059"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            18..929
                     /locus_tag="PWY43_001396"
     CDS             18..929
                     /locus_tag="PWY43_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL2136271.1"
                     /translation="MYWHIGKRIFEQEQQGQERADYGTYLIKMLAKELEPEFGSGFCS
                     RQLERYRQFYRAFPIATALRSQLNWTQYRRLIQISDSDKREFYLAESCKNNWSSRQLE
                     RQINSSLYERLLLSNDKESVLAVARNEQQPVDPKQLIKDPMVLEFLGLKRESAYYEKD
                     LEQAIITHLQDFLLELGNGFSFIARQKRLHLDGDDFFVDLVCYNRLLQCFVIFEIKTH
                     KLTHQDLGQLQMYVNYFDRVEKLPHENATIGVLLCADKNDAVVKFSLPENQNKIFASQ
                     YQLYLPSEQTLIAEVQKEIENFEQKYP"
     gene            1003..2640
                     /gene="ubiB"
                     /locus_tag="PWY43_001397"
     CDS             1003..2640
                     /gene="ubiB"
                     /locus_tag="PWY43_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDL2136272.1"
                     /translation="MNIKNLNRFYHIIRTFLRYGIDEAIPNLPITRKIKLGRKALFWV
                     KNQHSDKPYGVRLRLALQELGPVWIKLGQMLATRRDLFAPELADQLALLQDNVEPFDG
                     KIARQIIEQALGGKLETWFENFDETALASASIAQVHTAKFNQNQPLAGKDVVLKVIRP
                     GIEPVIKADLELMYEIAHFIPKLSNDGKRLRAVEVVREYEKTILDELDLRKEMHNAIR
                     LRANFENSEMLYVPEMYQDFCHKNLIAMERIYGIPVSNIAELEANSTDMKLLAERGVQ
                     VFFTQVFRDSFFHADMHAGNIFVNREHPENPQYIGIDCGIVGTLNHNDKRYLAESFVA
                     FFNRDYRRVALMHVESGWTPPDTDIDKFEQAFREVCEPIFAKPLSEISFGHVLLNLFN
                     VAREFNMEVQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAMV
                     RDIKQRLPQYREHFAEFPEALFKALQEQKQINFRLAEINESLKAQNSNANSRFLVLSI
                     SAIVICGAFWKFESLPIWLSVPLLTLFPLVLILSQRK"
     gene            2751..2972
                     /gene="tatA"
                     /locus_tag="PWY43_001398"
     CDS             2751..2972
                     /gene="tatA"
                     /locus_tag="PWY43_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDL2136273.1"
                     /translation="MGGISIWQLLIVVAIIVLLFGTKKLRTLGSDLGESVKGFKKAMA
                     DEKKDAEFEKVEAKDAISAEQKAKEKEQA"
     gene            2976..3620
                     /gene="tatB"
                     /locus_tag="PWY43_001399"
     CDS             2976..3620
                     /gene="tatB"
                     /locus_tag="PWY43_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDL2136274.1"
                     /translation="MFDIGFSELVLIMIVGLVVLGPKRMPIAIRTVMGWVSTIRGLAA
                     NVQNELAQELKLQELKESIKKAEELNLSSLSPELNKTVEDLKMSATQLQDSLNSAKNE
                     LEKTAAESSTIPKLTDEQVAEIQQKIEQESQVLEAQELQKNAQNKPLEVDSLSEQLNV
                     TRTEKPLSADELAEQAEMDESLLTLESYYPPDDDLATPQSSESAQKSEANNVAS"
     gene            3607..4362
                     /gene="tatC"
                     /locus_tag="PWY43_001400"
     CDS             3607..4362
                     /gene="tatC"
                     /locus_tag="PWY43_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDL2136275.1"
                     /translation="MSQVDESQPLITHLIELRNRLLRSFICVLLVFCGLVYWANDIYT
                     LLATPLTDNLPDGATMIATNVATPFFTPIKLTIVVSVFLSVPFILYQIWAFVAPALYK
                     QEKRLVYPLLVSSTLLFYCGVAFAYYIVFPLVFGFLTSTAPEGVTMATDISSYLDFVL
                     TIFMAFGICFEVPVAIILLCWAGVTTPEDLKAKRPYIIVAAFVIGMLLTPPDIFSQTL
                     LAIPMCLLFEVGVLFARFYRPKDDDEPSEQVAQ"
     gene            4443..5462
                     /gene="hemB"
                     /locus_tag="PWY43_001401"
     CDS             4443..5462
                     /gene="hemB"
                     /locus_tag="PWY43_001401"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDL2136276.1"
                     /translation="MQVIQSQYPLCRMRRLRKHDFSRRLVAENQLTANDLIYPVFVIE
                     GENQRVQVPSMPGVERLTIDQLLVEAAELVKYGVPAVALFPVVGDAKKSLMAEEAYNP
                     QGLAQRAVRALKAQFPELGVITDVALDPFTTHGQDGIIDETGYVLNDVTTEVLVKQAL
                     SHAEAGADIVAPSDMMDGRIGKIREALEQNGFINTQIMAYSAKYASNYYGPFRDAVGS
                     AGNLKGGNKYTYQVDPANGNEGLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKENFGV
                     PTFAYQVSGEYAMHMAAIQNGWLKERECIMEGLLCFKRAGADGILTYFAKRVAKWLYE
                     EAQAK"
     gene            5608..6558
                     /locus_tag="PWY43_001402"
     CDS             5608..6558
                     /locus_tag="PWY43_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL2136277.1"
                     /translation="MNSRILATGSYLPTKVRTNADLEKMVDTSDEWIVTRSGIRERRI
                     AEPDETVATMGAKAAQQALEMAGIEANDIDLIVVGTTTNSHAYPSAACQIQGLLQIED
                     AIAFDVAAACTGFVYALTVADQFVRNGQVKKALVIGSDINSRMLDETDRSTVVLFGDG
                     AGAVILEASEEPGIISTHLHASPDLENNLTLRSQKRGEERSGFIEMQGNATFKIAVNQ
                     LSSVVEETLEANNLQKSDLDWLIPHQANLRIITATAKKLEMEMDQVVVTLDKYANTSG
                     ATVPVALDEAVRDGRIQRGQLLLLEAFGGGWTWGSVLVKF"
     gene            6568..7317
                     /gene="trmB"
                     /locus_tag="PWY43_001403"
     CDS             6568..7317
                     /gene="trmB"
                     /locus_tag="PWY43_001403"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL2136278.1"
                     /translation="MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQ
                     RDMMNNNWANLGLDYQPMPFNFEQIFGNNNPVVLEIGFGMGKSLVEMAEQNPNRNYIG
                     IEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDSIADGTLGGLQLYFPDPWQKAK
                     HHKRRIVQPEFISRVIRKLSPNGFIHFATDWENYAEHMLEVLRQFESELQNTSATNDF
                     IPRPDFRPLTKFEERGHRLGHGVWDLYFTKK"
     gene            7341..7682
                     /locus_tag="PWY43_001404"
     CDS             7341..7682
                     /locus_tag="PWY43_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDL2136279.1"
                     /translation="MAIQRNARQRKKMHLGEYQELGFLIKFTFAEGTGIDQIDETVDR
                     FIDEVIRPNGLALEANGYLNWEGLVCLEKLGKCDESHRQLVKTWLETNGLKEVQVSEL
                     FDIWWDYPAQA"
     gene            7788..8321
                     /locus_tag="PWY43_001405"
     CDS             7788..8321
                     /locus_tag="PWY43_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL2136280.1"
                     /translation="MEFLAPVISLIVGFLSFVLVLRTWLQFCRVDPRLPISQTLLKIT
                     EPMVSPVGKLIPTVKNICFPALLIAAALVTLQFILFGVDLPRAVLIGLLSVVKTFGQI
                     LFFTTLIRALMSWVTQGNHPLDYTVAQITEPVLGFIRKLLPRTGMLDFSVMVLGFGLL
                     LLNSLFYQIFGGLWAAA"
     gene            complement(8537..9289)
                     /locus_tag="PWY43_001406"
     CDS             complement(8537..9289)
                     /locus_tag="PWY43_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2136281.1"
                     /translation="MLKIRNIHKTFGNNAILRGIDLDIQKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEQGTLEFSGNAPLKIDFAVKQSKKEILALRRKSGMVFQNYNLFPHKTAL
                     ENVMEGPVFVQQQAVEIAQKNAKALLEKVGLADKAELYPFQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNTMKELANEGWTMVVVTHEIKFALDVADVVVVMDG
                     GEIVEQGSPQQLFNHPQHERTKRFLNQIRAGK"
     gene            complement(9299..10015)
                     /locus_tag="PWY43_001407"
     CDS             complement(9299..10015)
                     /locus_tag="PWY43_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2136282.1"
                     /translation="MLNDLLLSIPFMTQARVDLVLSGFWPMVQAAFLVSIPLAIASFI
                     IGMLIAVAVALVRISPATGFFHRLLLWLVKGYISIIRGTPMLVQICIVFYGLPAIGIF
                     IDPIPAAIIGFSLNIGAYGSETIRAAISSVPKGQWEAGYTIGMTYMQTFRRIIAPQAF
                     RVAVPPLSNTFIGLFKDTSLASVVTVTEMFRVAQQVANMSYDFLPVYIEAGLIYWCFC
                     WVLFLIQARLEVRFNRFVAK"
     gene            complement(10098..10871)
                     /locus_tag="PWY43_001408"
     CDS             complement(10098..10871)
                     /locus_tag="PWY43_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2136283.1"
                     /translation="MLKKLSIAALALTLSVSAFAKESLLERINNKGTITVGTEGTYAP
                     YTYHDASGKLTGYDVEVTRAVAEKLDVKVEFKETQWDAMLAGLKGERFDLVANQVALT
                     SPERRATFDKSESYSWDGAALLVREDETKIKTPADVKGVKTAQTLSSNYGEIARENGA
                     DIVPIDGMAQGLLLIQQKRADATFNSHLSLLDYLKKNPNSGLKIVWEKEEKVGAGLIA
                     NKGNDEALAKISKAIVELREDGTLKKLGEQFFGKDISVQ"
     gene            complement(11051..11176)
                     /gene="ykgO"
                     /locus_tag="PWY43_001409"
     CDS             complement(11051..11176)
                     /gene="ykgO"
                     /locus_tag="PWY43_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDL2136284.1"
                     /translation="MKILNSLKTAKSRHPDCQIVRRKGKLYVICKTNPRFKARQR"
     gene            complement(11185..11457)
                     /locus_tag="PWY43_001410"
     CDS             complement(11185..11457)
                     /locus_tag="PWY43_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL2136285.1"
                     /translation="MKKGIHPENYREVLFYDASVQQGWVIRSCAATNKTMVWEDGKEY
                     PLYTLDTSSASHPVYTGKRREVNTEGRASQFKDRFKGFASTLSNQK"
     gene            11651..12328
                     /gene="rpe"
                     /locus_tag="PWY43_001411"
     CDS             11651..12328
                     /gene="rpe"
                     /locus_tag="PWY43_001411"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2136286.1"
                     /translation="MSKNPYLIAPSILSADLARLGDDVAEVLKAGADLIHFDVMDNHY
                     VPNLTFGPAICKALRDYGIQCPIDVHLMVKPVERLIPDFAKAGADYITFHPEATEHID
                     RNLQLIRDLGCKSGLVFNPATPLSYLDYVMDKVDVVLLMSVNPGFGGQSFIPSTLDKL
                     KEVRRRIDESGYNIRLEVDGGMKINNIADIAAAGADMFVAGSAIFDQPDYQAVIAQMR
                     DELRKVG"
     gene            12515..12745
                     /gene="acpP"
                     /locus_tag="PWY43_001412"
     CDS             12515..12745
                     /gene="acpP"
                     /locus_tag="PWY43_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2136287.1"
                     /translation="MSIEERVKKIIVDQLGAKAEDVKPEASFIEDLGADSLDTVELVM
                     ALEEEFDIEIPDEEAEKITTVQSAIDYVQNNQ"
BASE COUNT         3743 a         2444 c         2861 g         3708 t
ORIGIN      
        1 atttccaacg tgtgctgatg tattggcaca tcggaaaacg gatttttgaa caggagcaac
       61 aagggcaaga gcgtgctgat tatggaacat acctgattaa aatgctcgca aaagagcttg
      121 agccagaatt tggaagtggt ttttgtagcc gtcaattaga gcgttatcgc cagttttatc
      181 gtgcgtttcc aattgcgacc gcactgcggt cgcaattgaa ttggacacaa tatcgtcgtc
      241 taattcaaat ctccgattct gacaaaaggg aattttattt ggctgaaagt tgtaagaata
      301 attggagttc acgccagctg gaacggcaaa ttaatagtag tctatacgag cgattattgc
      361 tcagtaatga taaggaaagt gtgcttgctg ttgccagaaa tgagcagcag ccggttgatc
      421 cgaaacagct cattaaagac ccgatggtat tggaatttct cggactaaaa cgggaaagtg
      481 cttattacga aaaggattta gagcaagcca tcattacgca tttgcaggat tttctactag
      541 aattgggtaa cggtttctcg tttatcgcac gccaaaagcg tttgcaccta gacggtgatg
      601 atttttttgt cgatttggtt tgttacaacc gtttacttca atgttttgtg atttttgaaa
      661 ttaaaaccca caagctcacc catcaagatt tggggcaatt acagatgtac gtcaattatt
      721 ttgaccgagt tgaaaaactg ccgcacgaaa atgccactat tggtgtcttg ctttgtgccg
      781 ataaaaatga tgcggtggta aaattttcgc taccggaaaa tcagaataaa atttttgcca
      841 gccaatacca actttacctc ccctctgaac aaacacttat tgcggaagta cagaaagaaa
      901 ttgagaattt tgagcagaaa tacccatagt agcccaacaa gcggttaaat ttttacagaa
      961 ttttgctaaa atcttcctaa ttaccattaa atatatattt caatgaacat caaaaatctc
     1021 aaccgctttt accatattat ccgcactttt cttcgctatg gtattgatga agccattccg
     1081 aatctgccaa ttacccgcaa aataaagcta gggcgtaagg ctttgttttg ggtgaagaat
     1141 caacattcag acaaacctta cggtgtacgt ttacgtttgg ctttgcaaga gcttggaccg
     1201 gtttggatca agctcgggca aatgttagct accagacgtg atctgtttgc accggagctt
     1261 gccgaccagc tcgccttgct acaagataat gttgaaccgt ttgacggcaa aatcgctcgt
     1321 caaattatcg agcaggcttt gggcggaaag cttgaaactt ggtttgaaaa ctttgatgaa
     1381 actgccttag cttctgcttc gattgcacag gttcacacgg caaaatttaa ccaaaatcaa
     1441 ccgcttgcag gcaaagatgt ggtgttaaaa gtgattcgcc cgggcattga gccggtgatt
     1501 aaggcggatt tagagttgat gtatgaaatt gctcacttta tcccgaaatt atcgaatgac
     1561 ggcaaacggc tacgggcggt tgaagtggtg cgagagtacg aaaaaactat tttagacgag
     1621 ctggatttac gcaaagaaat gcacaatgcc attcggcttc gggcgaattt tgaaaatagc
     1681 gaaatgctgt atgtgcctga aatgtatcaa gatttttgcc ataaaaactt gattgcgatg
     1741 gagcggattt atggcattcc agtgtctaac attgccgagt tggaagcgaa cagcaccgat
     1801 atgaaattgc tggcagagcg tggtgtgcag gtatttttta cccaagtatt ccgtgacagt
     1861 tttttccacg ccgatatgca cgccggcaat atttttgtga acagagaaca cccggaaaat
     1921 ccgcaatata tcggcattga ctgcgggatt gtcggcacgt tgaaccacaa tgacaaacgc
     1981 tatttagccg aaagttttgt cgcatttttc aaccgagatt accgccgtgt tgccttaatg
     2041 cacgtggaat cgggctggac gccgccggat accgatattg ataaatttga gcaagccttt
     2101 cgtgaggtat gtgagccgat ttttgctaag ccgttatcgg aaatttcatt cggacacgtt
     2161 ttgctcaacc tgtttaatgt ggcaagagaa tttaatatgg aagtgcagcc gcagttggtg
     2221 ttgttgcaaa aaacacttct ttatattgaa ggtttgggca ggcaggttta tccacagttg
     2281 gatttatggc aaaccgcaaa accgttttta caaaactggc tgaatgagca ggtaggtgtg
     2341 aaagcgatgg ttcgagacat taagcaacgt ttaccgcaat atcgggagca ttttgcggag
     2401 tttccggagg cattgtttaa agccttacaa gagcaaaagc aaattaattt ccgtttagcg
     2461 gaaattaacg aaagtttaaa agcacagaat tcaaacgcaa atagccgttt tcttgtgctg
     2521 tcaatttcag cgattgtgat ctgtggcgca ttttggaaat ttgaatcttt gccgatatgg
     2581 ctaagtgtgc cgttattaac tttatttcca ctggtattga ttttgagtca acgcaaataa
     2641 gcggttgttt ttgacaaatt ttttgcaaaa ttactttttt atatgtcaaa gtaacatata
     2701 ataaacagcg ttttatttta tctattccct attaaatgaa aggagttttt atgggcggta
     2761 ttagtatttg gcagcttctg attgtggttg caatcatcgt attacttttc ggtacgaaga
     2821 aattacgtac tttaggttcg gatttgggcg aatctgtaaa aggcttcaaa aaagcaatgg
     2881 cagatgagaa aaaagatgct gaatttgaaa aagtggaagc aaaagatgca atttcagcag
     2941 agcagaaagc aaaagagaaa gagcaggctt aatccgtgtt tgatataggt ttttctgaat
     3001 tagtactgat tatgattgtg ggcttggtgg tgctaggccc gaaacgtatg ccgattgcga
     3061 ttcgtaccgt aatgggctgg gtgagtacaa ttcgaggttt agccgcaaac gtacaaaatg
     3121 aactggctca agagctgaaa ctgcaagaat taaaagagag cattaaaaag gcagaagagt
     3181 tgaatttaag ctccctttcg ccagaattaa ataaaacggt ggaagatctg aaaatgtcag
     3241 ccacccagtt gcaagattcg cttaattctg caaaaaatga gttggaaaaa acggctgcgg
     3301 aaagcagcac aattcctaaa ttaaccgatg agcaagtcgc agaaattcag caaaaaatcg
     3361 agcaagaaag ccaggtgtta gaggcgcaag aattgcaaaa aaatgcgcaa aataaaccgc
     3421 ttgaagtgga tagtttatct gagcaactta atgttacgcg gacagaaaag ccactttcgg
     3481 ctgatgagct ggctgaacaa gcagaaatgg acgaatcgct tttaacgttg gaatcttact
     3541 atccgccaga tgatgattta gctacgcctc aatcatcaga atcagcgcag aaatcggagg
     3601 caaataatgt cgcaagttga tgagtctcaa cctctgatta cacatttaat tgagctgaga
     3661 aaccgattac tgcgtagttt tatctgtgtg ctcttggtgt tttgcggatt ggtttactgg
     3721 gccaatgata tttacacctt attggcaaca cctttaacgg acaatttacc agacggtgca
     3781 acaatgattg caaccaatgt agccactccg ttttttaccc caattaaatt aaccattgtg
     3841 gtttcggtgt ttttatcagt tccttttatt ttgtaccaaa tttgggcgtt tgtcgcccct
     3901 gctttataca agcaggaaaa gcgtttggtt tacccattat tggtatctag cacattgctg
     3961 ttttattgtg gtgtcgcttt tgcctattat attgtgtttc cgcttgtatt tggcttttta
     4021 acctctaccg ctccggaagg ggtaacaatg gcaacggata ttagtagcta tcttgatttc
     4081 gttttaacca tttttatggc attcgggatc tgctttgaag tgccggttgc gattatcttg
     4141 ttatgttggg caggcgtaac cacacccgaa gatttaaagg caaaacgccc ttatattatt
     4201 gtggcagcat ttgtaattgg tatgttgctc accccaccag atattttctc gcaaacatta
     4261 ttggcaattc caatgtgttt attgtttgaa gttggtgtgc tatttgctcg gttttatcgc
     4321 cccaaagatg atgatgaacc atcagagcag gttgcacagt aatattttga aaaggcatag
     4381 cacctgtttg ctatgccttt cttatttcaa gaataggtat tttaagaatt aggaaattaa
     4441 aaatgcaagt aattcaatcc caatatcctt tatgccgtat gcgtcgttta cgcaagcacg
     4501 atttcagccg ccgcttagtg gcagaaaatc aacttaccgc taatgatttg atttaccctg
     4561 tttttgtcat cgaaggggaa aatcagcgag tacaagtgcc ttctatgccg ggtgtagagc
     4621 gtttaactat cgaccagctt ttagttgaag ccgcagaact ggttaaatat ggcgtgcctg
     4681 cggtggctct tttccctgtg gtgggtgatg cgaaaaaatc actgatggca gaagaggcct
     4741 ataacccgca agggttggca cagcgtgccg tgcgagcttt aaaagctcaa tttccggaat
     4801 taggcgtgat tactgatgtg gcgttagacc cgtttaccac gcacggacaa gacggcatta
     4861 ttgatgaaac aggctatgta ctgaatgatg tgactactga agtattggta aaacaggcgt
     4921 tatcgcacgc agaagcgggg gcggatattg ttgccccgag cgatatgatg gacggtcgaa
     4981 tcggcaaaat tcgtgaagcc cttgagcaaa acggttttat caatacgcaa attatggcgt
     5041 attcggctaa atatgcgtct aattattacg gaccgttccg tgatgcagta ggttctgccg
     5101 gcaacttaaa aggcggtaat aaatacactt accaagtcga tccggctaac ggcaacgaag
     5161 gcttgcacga agtggcgatg gatattcaag aaggggcaga tatggtgatg gtgaaaccgg
     5221 gtatgcctta tttggatatg gtgtggcgtg tgaaagagaa ttttggcgtg ccaaccttcg
     5281 cctaccaagt ttcgggcgaa tacgcgatgc atatggcggc aatccaaaac ggctggctga
     5341 aagagcgtga gtgcattatg gagggattac tctgctttaa acgggcagga gcagacggta
     5401 ttctaactta tttcgccaag cgagtagcga agtggctata cgaagaggct caagcgaaat
     5461 aaatactagc aagaggtggt tctagtgcaa aatcgttcgt gaatgcaact tttttgctaa
     5521 aaatgaccgc ttgtaaagcc tatatatttg ttggttttca agctataata acggattatc
     5581 atttaattga ttgagaataa taaaaatatg aatagtagaa ttttagcaac gggaagctat
     5641 ttaccgacaa aggtgcgtac caatgctgat ttagaaaaaa tggtcgatac ctctgatgaa
     5701 tggatcgtaa cccgttccgg cattcgtgaa cgccgtattg ccgagcctga cgaaacagtg
     5761 gcaacaatgg gggcaaaagc cgctcaacaa gcactggaaa tggcaggaat tgaggcaaat
     5821 gacattgatt taattgtggt gggaacaact accaactccc acgcttaccc aagtgcagct
     5881 tgccaaattc aaggtttgct tcagattgaa gatgcgattg cttttgatgt ggcggcggct
     5941 tgtaccggct ttgtgtatgc tttaactgtc gctgatcaat tcgtgcgtaa cgggcaagtg
     6001 aaaaaggcat tggtgattgg ttcagacatc aactccagaa tgctggacga aaccgaccgc
     6061 agcacggttg tgttatttgg tgacggagca ggggcggtga ttttagaagc gagtgaagag
     6121 ccaggtatta tttcgaccca tttacacgct tcgccggatt tagagaataa cttaacttta
     6181 agaagccaaa aacgtggcga agagcgttcc ggctttattg aaatgcaagg caatgctact
     6241 tttaaaattg cggtaaacca actttcaagc gtggtagaag aaaccttaga agcgaataat
     6301 ctacaaaaat ccgatttaga ttggctgatt ccgcaccaag cgaatttacg cattattacc
     6361 gcaacggcga aaaaattaga gatggaaatg gatcaagtgg tggttaccct tgataaatat
     6421 gccaatacaa gtggagcaac agtgccggtg gcattagatg aagcggtgcg tgacgggcga
     6481 attcaacgtg ggcaattact actccttgaa gcattcggcg gtggctggac ttggggttcg
     6541 gtattagtga agttttaagg caagaaaatg tcagaaaaac agacttttgc agataaaaaa
     6601 cgtaaaaccg ttgagcaagc cgagtttacc gaagatgggc gttatttacg aaaagtgcgg
     6661 agttttgtat tacgtaccgg tcgtttgagc gattatcaac gtgatatgat gaacaacaac
     6721 tgggcaaatt taggcttaga ttaccagcca atgcccttta attttgagca gattttcggc
     6781 aataacaacc cggtagtgtt agaaatcggc ttcggaatgg gaaaatcgct ggtggaaatg
     6841 gcagagcaaa acccgaatcg caactatatc ggcattgagg tgcacacgcc cggggtgggg
     6901 gcttgtatcg cttatgcgtt agaaaaaggc gtgaagaatt taagagtaat ttgccacgat
     6961 gccaccgaaa ttttgcgaga cagcattgct gacggtacat taggtggctt acagctttac
     7021 ttcccagacc cttggcaaaa agcaaaacac cacaaacgcc gtattgtgca gcctgaattt
     7081 atcagccgtg tgatcagaaa attaagccct aacggcttta ttcattttgc caccgactgg
     7141 gaaaattacg cagaacatat gcttgaagta ttacgccaat ttgagagcga gttgcaaaat
     7201 acatcagcaa ctaatgattt tattccacgt ccggatttcc gcccgctaac caaatttgaa
     7261 gagcgagggc atcgtttagg acacggggtg tgggatttat attttacaaa gaaatgattt
     7321 attcatagag gagagaaaaa atggctattc aacgtaacgc acgccaacgt aaaaaaatgc
     7381 acttaggtga ataccaagaa ttaggtttct taattaaatt cacctttgct gaaggcacag
     7441 gcattgacca aattgatgaa accgtagatc gttttatcga tgaagtgatt cgaccaaacg
     7501 gtttagccct tgaagcgaac ggctacttaa actgggaagg tttagtctgc ttagaaaaat
     7561 taggcaaatg tgatgaatct caccgccaat tagtgaaaac ttggttagaa actaacggct
     7621 taaaagaggt tcaagtgagc gaattatttg atatttggtg ggactaccca gcacaagctt
     7681 aatttgccta gttaatttaa taagatacaa gcggtcagat tttactgaaa atttgcaaat
     7741 tttttctaaa aaacgaccgc ttgttttgta tagaaagaga aaaagagatg gaatttttag
     7801 ctcctgttat ttcgcttatt gttggctttt taagttttgt gctggtgcta agaacttggc
     7861 tgcaattttg tcgggttgat cctcgtttac cgatttcaca aaccttactt aaaattaccg
     7921 aaccgatggt ttcaccggtt ggcaagctga ttccaaccgt taaaaatatt tgctttccgg
     7981 cactattaat tgccgccgct ttggtaacgt tacagtttat tctgtttggc gtagatttac
     8041 ccagagcagt attaatcggc ttattaagtg tggtcaaaac cttcggacaa attctatttt
     8101 tcaccacctt gattcgtgca ttaatgagct gggtcactca aggcaaccac ccattagact
     8161 acacggtagc acaaattacc gaaccggtgt taggttttat ccgcaagctc ctgccaagaa
     8221 cgggaatgtt agatttctcg gtaatggtgc taggtttcgg cttattgcta ctaaatagct
     8281 tgttctacca aatttttggt ggactatggg ctgcggctta aatcggattt ctaaaatcta
     8341 aaaataagct ccttaatcta aaagattaag gagccttgtc ttatttatcc tttatgggga
     8401 accttgttag ctgattttac tgtacgatgg cgcgagcgtc cacgctcgtg ccaaaacagt
     8461 ataacaattt atacataatt aaccgcttgt accatttatt agatagcact agcgtggacg
     8521 ctagcgctat cgtttcttat ttacccgctc tgatttgatt taaaaaacgt ttagttcgtt
     8581 cgtgttgtgg gtggttaaac agttgctgtg gcgagccttg ttcaacgatt tcaccgccgt
     8641 ccattaccac cactacatcg gcaacatcaa gggcaaattt gatttcgtga gtaaccacga
     8701 ccattgtcca gccttcattt gccagttctt tcatcgtatt tagcacgtct tgcaccaact
     8761 caggatcaag tgccgaagtt ggttcatcaa ataacatcag ctccggctga attgctaatg
     8821 ctcgtgcaat gccaacccgt tgctgttgcc cgcctgacag ttgaaacggg tagagttctg
     8881 ctttatccgc cagtccgact ttttctaaca gggcttttgc atttttttga gcaatttcga
     8941 ccgcttgttg ttgcacaaat accggtcctt ccatcacatt ttccaatgcg gttttatgcg
     9001 ggaacaggtt gtagttttgg aacaccatac cggatttgcg gcgtagcgct aaaatctctt
     9061 ttttgctctg ttttacggca aaatcaattt tcagcggagc attaccacta aattccaatg
     9121 tgccttgctc gggcatttcc agtgcattta agcaacgaag aaaggtggtt ttgcctgaac
     9181 cggaagggcc taaaattact accacttgcc ctttttgaat atctaaatca atgccgcgta
     9241 gaatagcgtt gttgccaaag gttttatgaa tattgcgaat tttaagcaca attcctcctt
     9301 atttcgccac aaatcggtta aagcggactt ccaatctcgc ctgaattaaa aatagcaccc
     9361 agcagaagca ccaataaatc aaaccggctt caatataaac aggtaggaag tcataagaca
     9421 tattcgctac ctgttgagca acccggaaca tttcggttac ggtaacaacg gaggcaagag
     9481 aggtatcttt aaacagaccg ataaaggtgt tgctaagtgg cggaacagca actcgaaacg
     9541 cttgcggggc aatgatacgg cgaaaagttt gcatataagt cataccgata gtataaccgg
     9601 cttcccattg tccttttggc acggaggaga tcgcagcacg gatcgtttcc gagccataag
     9661 ccccgatatt gagtgaaaac ccgataattg ccgccggaat cggatcgata aaaatgccga
     9721 ttgcaggcaa accataaaac acaatacaga tctgaaccaa catcggcgtg ccgcgaataa
     9781 tcgagatata accttttacc agccacagta gcaagcggtg aaaaaagcct gttgcagggc
     9841 taattcgcac taatgcaact gcaacggcaa ttaacatacc gataataaat gaggcgattg
     9901 ccagcggaat agagactaaa aatgccgctt gtaccatcgg ccaaaaaccg cttagcacta
     9961 aatcgacccg agcttgggtc ataaagggga ttgagagaag tagatcgttt aacatctgaa
    10021 aaacctaaaa tctcccgttt tgagggagat ttaaaaataa aaggggcgaa ttcgccccaa
    10081 gcctaatcca atatggatta ttgtacacta atatctttac cgaagaactg ctcgcccaat
    10141 ttttttaacg tgccgtcttc acgtaattct acgattgctt tgctgatttt cgctaatgct
    10201 tcgtcattgc ctttattcgc aattaagcct gcacccactt tttcttcttt ttcccacaca
    10261 atttttaagc cggaattcgg gttctttttc aagtaatcta atagcgacaa gtggctattg
    10321 aaagttgcat cggcgcgttt ttgttgaatt aacagtaagc cttgagccat accgtcaatt
    10381 ggcacgatgt ctgcaccgtt ttcacgggca atctcgccat agtttgagct caaggtttgg
    10441 gcagttttca cgccttttac atcagcaggg gttttgattt tagtttcatc ttcacgcact
    10501 aataaggcgg caccgtccca gctgtaagat tcggatttat caaatgtcgc tcgacgttcc
    10561 gggctggtta aagccacttg gtttgccact aagtcaaaac gctcaccttt taaaccggct
    10621 aacatagcgt cccattgtgt ctctttaaat tccactttta cgtctaattt ttcagccacc
    10681 gcacgggtta cttctacatc gtaaccggtt aatttaccgc tcgcatcgtg ataggtgtaa
    10741 ggggcataag ttccttccgt acccacagta atagtccctt tgttgtttat acgctcaagt
    10801 aggctctctt tagcaaatgc agaaacagaa agggtaagag ctaatgcagc aattgataat
    10861 tttttcaaca taatcatttc cttttaattg gtaaaataaa ttttttgcaa atttaagggg
    10921 aaaattgacc gcttgcaaat aactaaatgg aataagaaat aataaaatcg ttgatgtggt
    10981 taaaataggt aatattgaga agatgaaata aaaaacacct taataaatta gcttattaag
    11041 gtgctggttt ttaacgctga cgagctttaa atctcgggtt ggttttgcaa atcacataaa
    11101 gtttgccttt gcgtctgaca atttggcaat ccgggtggcg tgattttgcg gttttaagtg
    11161 agtttaagat tttcatttta tcctctattt ttgatttgaa agcgttgaag caaagccttt
    11221 aaatctatct ttaaattgac ttgcacgacc ttctgtattc acttcacggc gtttacctgt
    11281 gtaaacaggg tgagacgccg atgaggtgtc taaggtgtaa agtgggtatt ctttgccatc
    11341 ttcccatacc attgttttgt tggttgccgc acaagaacgg ataacccagc cttgttgcac
    11401 tgaggcatca tagaacaaca cttcacgata attttcagga tgaatccctt ttttcatatt
    11461 ttctccagaa tttagataag gtcattaacc ataattaaaa tatgggatac ataatatacg
    11521 aactttaaat acaattccat aactttttta tggtgttttg taaaaaaacg gatttttagg
    11581 aagagagact cttttcgctg ttaaaattat ggtaatctaa cgtagattta ttttttgata
    11641 gtgaggaaat atgagtaaga atccttattt aattgcaccg tctattttat ctgccgattt
    11701 agctcgcttg ggtgatgatg tggcagaggt gttaaaagcc ggtgcagact taattcactt
    11761 tgatgtaatg gacaaccact acgtgccgaa tttgaccttc gggccggcga tctgcaaagc
    11821 cttacgtgat tacggcattc aatgcccgat tgatgtgcat ttaatggtaa aaccggttga
    11881 gcgtttgatt ccggattttg ccaaagccgg agcggattat attacattcc accctgaagc
    11941 aacggaacat atagatcgaa atttacagct tatccgtgat ttaggttgta aatcaggctt
    12001 ggtgtttaac ccggcaacgc cattgagcta tttagattat gtgatggata aagttgacgt
    12061 ggttttattg atgtctgtga atccgggctt tggcggacaa tcatttattc cgtccacctt
    12121 agataaattg aaagaagtcc gtcgtcgtat tgatgagagc ggttataaca ttcgcttaga
    12181 ggttgatggt ggtatgaaaa taaataacat tgccgacatt gcagccgcag gagcagatat
    12241 gtttgtggca ggttctgcga tttttgacca gccagattat caagcagtga ttgcacaaat
    12301 gcgagatgaa ttacggaaag tagggtaatc aaatgaaatt tgatttatct ctatagatta
    12361 aacacaaaca taatgataga atggaaaaac agatatattc tggtgtatag gaatagtctg
    12421 gtttgacctt tttataaaag gttgtattta aacttatttc gattacacta tttaactctc
    12481 gcacttatgc gatattcact ataggaaaaa caaaatgagc attgaagaac gcgtaaaaaa
    12541 aatcattgtt gatcaacttg gcgcgaaagc agaagatgta aaaccagaag cttcattcat
    12601 tgaagattta ggtgcagatt ctttagacac tgttgaatta gtaatggctt tagaagaaga
    12661 attcgatatc gaaattccag atgaagaagc tgaaaaaatt actacagttc aatcagcaat
    12721 tgattacgtt caaaacaatc aataatctga attaat
//
LOCUS       DANKBV010000060        12675 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.060, whole genome shotgun sequence.
ACCESSION   DANKBV010000060 DANKBV010000000
VERSION     DANKBV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12675
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.060"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            84..434
                     /locus_tag="PWY43_001413"
     CDS             84..434
                     /locus_tag="PWY43_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2136288.1"
                     /translation="MNTVISQRKPIHWVGSTLDDIREFPDNIKRELGFDLDLVQQGLL
                     PRDFKAMANLGSGVMEIRVKDPSGAFRLVYLAKFKDAIYCLHAFQKKTQKTAPKDIAV
                     IKARYAAVKELENE"
     gene            427..750
                     /locus_tag="PWY43_001414"
     CDS             427..750
                     /locus_tag="PWY43_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2136289.1"
                     /translation="MNNIDTKIRHTTEAQGNVFEDLGFPIDEAQKLKSASQQLIETKL
                     MLMNEMSNWIDKNNLKQSEAASILGVSRPRISDMVNGKLEKFTLDTLVTFVAKTGKTI
                     QLQLS"
     gene            858..1337
                     /gene="smpB"
                     /locus_tag="PWY43_001415"
     CDS             858..1337
                     /gene="smpB"
                     /locus_tag="PWY43_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL2136290.1"
                     /translation="MAKKPKVASNTIALNKRARHEYFIEDEIEAGLELQGWEVKALRA
                     GKGNISDSYVIFRHGEAFLFGAMITPLNMASTHIVSDPTRTRKLLLNKRELDSLFGKV
                     NREGYTIVATSMYWKNAWVKVKIGLAKGKKLHDKREDIKDREWQVAKQRIMKNANRG"
     gene            1540..2847
                     /gene="serS"
                     /locus_tag="PWY43_001416"
     CDS             1540..2847
                     /gene="serS"
                     /locus_tag="PWY43_001416"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL2136291.1"
                     /translation="MIDQNLLRTNLEDVATALKIKRNFILDVERVKTLEEQRKTLQVK
                     TETLQAERNTRSKNIGAAKARGEDISALLAEVDSMGNELDTAKVELDKVQAEIRELLL
                     SVPNLPADEVPLGKDDSENLEVLRWGEPRQFDFEVQDHVTLGENLNGLDFAAGVKLTA
                     SRFVVMKGKLARLHRALSQFMLDLHTEQHGYVETNVPFLVNHDTLYGTGQLPKFGEDL
                     FHTQPLTGQDPNEVQRPFGLIPTAEVPVTNLVRDEIIDEDSLPLRYTAHTPCFRAEAG
                     SYGRDTRGLIRMHQFEKVEMVQIVAPEKSMEALEELTGHAEKVLQLLGLPYRKVLLCT
                     GDMGFGAAKTYDLEVWLPAQNTYREISSCSNMWDFQARRMSARCKAKGDKKTRLVHTL
                     NGSGLAVGRTLVAVLENYQNADGSITVPEVLRPYMGGLEVITA"
     gene            complement(2906..3232)
                     /locus_tag="PWY43_001417"
     CDS             complement(2906..3232)
                     /locus_tag="PWY43_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136292.1"
                     /translation="MVSVDFKNNLLEFIFYGLFSEHTQTNIINGLQFYPKAGVAFRKD
                     LRFRKRAVYQREIMVFYQLDKIDEPKKVTIIAFRLGRMKNPIRLDTVQNRRVVTEIFM
                     NYPEKD"
     gene            complement(3225..3437)
                     /locus_tag="PWY43_001418"
     CDS             complement(3225..3437)
                     /locus_tag="PWY43_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136293.1"
                     /translation="MSIVIVDTDFVVHKEDEEWTTEEIKMIEEHLLEEQSAQYQVKRE
                     VPKQFYADIAERLSRGDLTAWEQLDG"
     gene            3624..6209
                     /gene="leuS"
                     /locus_tag="PWY43_001419"
     CDS             3624..6209
                     /gene="leuS"
                     /locus_tag="PWY43_001419"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL2136294.1"
                     /translation="MQQQYNPSEIEPKVQRYWAENKVFKAVKDVTKEKYYCLSMLPYP
                     SGKLHMGHVRNYTIGDVVSRYQRMIGKNVLQPMGWDAFGLPAEGAAVKNNTAPAKWTY
                     ENIEYMKGQLKMLGFSYDWDREVTTCRPEYYKWEQWFFTELYKKGLVYKKTSTVNWCP
                     NDETVLANEQVHEGCCWRCDTPVEQREIPQWFIKITDYAEELLNQLDNLPQWPDQVKT
                     MQRNWIGRSEGVEITFKIAGSNVELPVYTTRPDTFFGVSYVAIAAAHPLAELAAQNNP
                     ALAEFIREAKNTKVAEAELATMEKKGMATGLFAIHPLTGKEVPVWVANFVLMHYGTGA
                     VMAVPAHDERDFEFAQKYDLQINQVIQPLDGSEWDFSKAAYTEHGKLINSAEFDGLEF
                     DVAFTAIADKLEAMGVGKRQVNFRLRDWGVSRQRYWGAPIPMMTTEEGEVVTVPLQDL
                     PVILPEDVVMNGVQSPIKADPEWAKTTYNGKPALRETDTFDTFMESSWYYARYTCPQY
                     HEGMLDSDEANYWLPVDQYIGGIEHATMHLLYFRFFHKLLRDAGILNSDEPATKLLCQ
                     GMVLADAFYYTSPTNERIWVSPTQVTLERDEKGRIIKATDPQRRELVHSGMTKMSKSK
                     NNGIDPQEMVEKYGADTVRLFMMFASPAEMTLEWQESGVEGAKRFLGRVWNLVYSYAQ
                     NPATTALDVNALDKDQKELRRDVHKTIAKVSDDIGRRQTFNTAIAAVMELMNKLTKAP
                     LETEQDKAVMAEALSAVVRMLYPITPHICFELWQVLGNEQTIDFAPWVQADESAMVED
                     EKLVVVQVNGKVRGKITVSATATEDEVKAIAKADPNVAKFLDGQQIVKEIYVPFKMLS
                     FAVKA"
     gene            6409..6897
                     /locus_tag="PWY43_001420"
     CDS             6409..6897
                     /locus_tag="PWY43_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136295.1"
                     /translation="MIKKLSLIAVFALTACGWHFKNNEVLPESFRTLTFESADQHSEM
                     SRILRNQLQLSDVKLVPSTANVAKLRLVSTSTNSKVVSVFKQAREAEKILTLNVDAIA
                     DIPNKGQYPLEVSVHRTFFDDSRAALAKSAEKEMIMKDMYEQASRQLIIKMAGLKKEL
                     SK"
     gene            6940..7965
                     /gene="holA"
                     /locus_tag="PWY43_001421"
     CDS             6940..7965
                     /gene="holA"
                     /locus_tag="PWY43_001421"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL2136296.1"
                     /translation="MQKIFPETLQFSLNKGLQPFYLLTGNDLLLVNETKDAIIHTARL
                     NGFDEKKEVEIKNDTNWEELFEAAQSMGLFFARQIMVLNFPDSPTATQFKKLAELCAF
                     IHSDLLFILHLPKFSKTMKKQAWFTQIELNLVQINCQTPELSKLPQWVSHRAKAMNLQ
                     IENEANQLLCYSYEGNLLALKQALQLLQLNATDGKITLTKAQEVVEHSAQFTPFQWID
                     ALFEGKIQRAQRILYHLQNEEVQAVVLLRIVQKELMILLEITRNPTAVVSSEQSLFIG
                     NLRTEFDRLKIWQNRRPFYNQITQRLTYQKLYALIQQLAELERKVKQEFSDDIWQELE
                     RFSAQFT"
     gene            complement(7966..8151)
                     /gene="yacG"
                     /locus_tag="PWY43_001422"
     CDS             complement(7966..8151)
                     /gene="yacG"
                     /locus_tag="PWY43_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDL2136297.1"
                     /translation="MPETIVNCPTCEKEVVWSKESKYRPFCSERCQIIDLGDWAAEKH
                     SIASEEAELFSEDLEKY"
     gene            complement(8133..8768)
                     /gene="coaE"
                     /locus_tag="PWY43_001423"
                     /pseudo
     CDS             complement(8133..8768)
                     /gene="coaE"
                     /locus_tag="PWY43_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248405.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
     gene            complement(8804..9460)
                     /locus_tag="PWY43_001424"
     CDS             complement(8804..9460)
                     /locus_tag="PWY43_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL2136298.1"
                     /translation="MLILFSLFIAVWLKAEIPRFAIRVNRQIYQETTSLVNIGLTEQQ
                     FLAQSVLQPKQPRSANLFFLLFPLIMLCSESPIIGTIFILLCFLSLLDMCYYLTDIRY
                     LVLIFLLVLWESLADFQHQTFTFTLAFCLLIGLVSQLMFKKEAFGSGDMLLLVALSPL
                     FRLEKMLLLILTASLLGIGYYLAYWLMKKQKLAKLAFIPFISTAMLLVLMLESRGINY
                     "
     gene            complement(9463..10659)
                     /locus_tag="PWY43_001425"
     CDS             complement(9463..10659)
                     /locus_tag="PWY43_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL2136299.1"
                     /translation="MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIR
                     ISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQALSMLLQNCRNIRLYLWLNELIQ
                     HIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
                     KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGT
                     VLLGTLLLAVIFYLFFAKKSRLVAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLN
                     AHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGINPNVFGAEMAQMLEV
                     GEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFD
                     MGALVE"
     gene            complement(10643..12046)
                     /locus_tag="PWY43_001426"
     CDS             complement(10643..12046)
                     /locus_tag="PWY43_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDL2136300.1"
                     /translation="MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAVPLQETAE
                     QLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSEELKFLLNALSPEQSSPQSEIYEEN
                     PLYPFSENTEQPDRNDPIIQILENLFKFCLQQNASDIHLEPQKERLLIRLRIDGVLHI
                     YKSLPSSLSSRLISRVKLLAKLDISETRLPQDGQFHFKTLLAETLDFRVSTLPTHFGE
                     KVVLRLQKNKPTHFDFLDLGFNPTQKANLLNALSQPQGLILVTGPTGSGKSITLYSAL
                     SHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTFLRQDPDIIMLGEIR
                     DEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLR
                     RVCVKCGGAGCEHCYQGYKGRIGVYQLLNRSAKNFEKNTACLDFKTLADSAKEKLTEG
                     KTNQAEILRVLGNDENL"
     gene            complement(12067..12546)
                     /gene="ppdD"
                     /locus_tag="PWY43_001427"
     CDS             complement(12067..12546)
                     /gene="ppdD"
                     /locus_tag="PWY43_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDL2136301.1"
                     /translation="MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNS
                     YTQKAALSELLQASASYKSDVELCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIA
                     VTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG"
BASE COUNT         3913 a         2654 c         2513 g         3595 t
ORIGIN      
        1 cggtgctaca atttttattg gcggatatgt ttgagtaaaa tcttgttgca tatttccatt
       61 aaaggtataa aattttatac attatgaata ctgtaatttc ccaacgaaaa ccaattcact
      121 gggttggatc aaccttagat gatattcgtg aatttccgga taacattaaa agagaactcg
      181 gttttgatct tgatttagtt cagcaaggct tattaccgag ggattttaaa gcgatggcaa
      241 atttgggttc aggcgtaatg gaaattcgag taaaagatcc ctcaggggct tttcgcttag
      301 tgtatttagc taaatttaaa gatgcaattt actgtttaca cgctttccaa aagaaaacgc
      361 aaaaaacagc ccctaaagac attgctgtca ttaaagcacg ctatgctgcc gtaaaggagt
      421 tagaaaatga ataatattga tactaaaatt cgccatacta cagaagctca gggtaatgtt
      481 tttgaagatc ttggtttccc tattgatgaa gctcaaaaat tgaaatctgc cagtcagcaa
      541 ctgattgaaa ctaaactgat gctaatgaat gaaatgagca actggattga taaaaataat
      601 ctcaaacaat cagaagcggc ttctattcta ggtgttagcc gccctcgaat ttctgatatg
      661 gtcaatggca agttagaaaa gtttacccta gataccttag tcacttttgt tgcaaaaacc
      721 ggcaaaacaa tccaattgca attatcctga tatttgcaaa attttactca aattcaaccg
      781 cttgttgctt ttatcattca ctaacaaggt tatttcaagt aaaattgtga ctaatttttt
      841 attaataaac acaaattatg gcaaaaaaac ctaaagttgc atcaaatacg attgcgttaa
      901 ataaacgtgc cagacacgaa tatttcattg aagatgaaat tgaagccggc ttagagctac
      961 agggctggga agtcaaagca ttgcgtgccg gaaaaggcaa tatcagcgat agctatgtca
     1021 tctttcgcca cggcgaagcc ttcttattcg gggcaatgat tacgcctttg aatatggcat
     1081 caacccatat tgtcagcgac ccgacccgca cccgcaaact tctactgaat aaacgtgagt
     1141 tagactcact gttcggtaaa gttaatcgtg aaggttatac cattgttgcc acctcaatgt
     1201 attggaaaaa tgcgtgggtg aaagtaaaaa tcggcttagc caaagggaaa aaactgcacg
     1261 ataaacgtga agatattaaa gaccgtgaat ggcaagtggc taaacagcgt attatgaaaa
     1321 acgccaatcg tggctaaatg aataattacc ctctcccctt gtgggagagg gacagaaaaa
     1381 ttgcgttcag caatttttca gggaaagggg gatttgtaaa agttcaagga aaaacaacgc
     1441 ttgccctctc tctgaaaaat atttctgaac gaaatatttt tcggtctctc tcccataaag
     1501 ggagagagtg aaacacaaat ttaaactaag gaacatacaa tgatcgacca aaacctactt
     1561 cgtaccaatt tagaagacgt tgctacagct cttaaaatca aacgtaattt tattttagat
     1621 gtagagcgag taaaaaccct tgaagaacag cgtaaaacgc tacaagttaa aaccgaaacc
     1681 ttacaagcag aacgcaacac tcgctccaaa aacatcggtg cggcaaaagc tcgtggcgaa
     1741 gatatttcag ctttattagc cgaagtagat tcaatgggca acgaattaga cacggcaaaa
     1801 gtagaattgg ataaagtaca ggcagaaatc cgtgagttgt tgctttctgt gccaaactta
     1861 ccggcagatg aagtaccact cggtaaagac gatagcgaaa acttagaagt attacgttgg
     1921 ggcgaaccac gtcaattcga ctttgaagtt caagatcacg ttacccttgg cgaaaactta
     1981 aacggtttag attttgctgc cggtgtaaaa ttaaccgcca gccgttttgt ggtaatgaaa
     2041 ggtaaacttg cccgcttaca ccgtgcatta tctcaattta tgttggattt acacactgaa
     2101 caacacggct atgtggaaac caatgtgcca tttttagtca atcacgacac tctatacggc
     2161 acaggtcagc taccaaaatt tggcgaagat ttattccata ctcaaccatt aacagggcaa
     2221 gatccaaacg aggtgcaacg cccattcggt ttgattccaa ccgctgaagt gccagtaacg
     2281 aacttagtgc gagatgaaat cattgatgaa gacagcttac cactgcgtta taccgcccac
     2341 accccttgtt tccgtgcgga agccggttct tatgggcgtg atactcgtgg tttaattcgt
     2401 atgcaccaat ttgaaaaagt ggaaatggtg caaattgtcg cccctgaaaa atcaatggaa
     2461 gccttagaag aattaaccgg tcacgcagaa aaagtcttac agcttttagg cttgccatac
     2521 cgcaaagtgc tactctgcac tggtgatatg ggctttggtg cagcaaaaac ctacgactta
     2581 gaagtttggc taccggcaca aaacacttac cgtgagattt cttcttgctc aaatatgtgg
     2641 gatttccaag cccgccgaat gtctgcacgt tgtaaagcta aaggcgacaa gaaaactcgc
     2701 ttggttcaca ccttaaacgg ctcaggttta gcggttggtc gcacactggt cgctgtgtta
     2761 gaaaactacc aaaatgccga tggctctatt actgtgcctg aggtgttaag accttatatg
     2821 ggtggtttgg aagtaattac cgcttaagat taataaatga aaccaatctc agaaatgggg
     2881 ttggtttttt taataaatct tatccctaat ctttttcagg ataattcata aaaatttcag
     2941 taactactcg gcggttttgc actgtatcta aacgaatagg atttttcatt ctccctaaac
     3001 gaaatgcaat aatggtgact tttttcggtt catctatttt atctaattga tagaatacca
     3061 ttatttcccg ttgataaacc gctcgctttc tgaaacgtaa atcctttcga aatgcgacac
     3121 ctgcttttgg ataaaactgt aaaccattga tgatattcgt ttgagtatgc tccgaaaata
     3181 atccatagaa aataaactct aatagattat ttttaaaatc aacactaacc atctaactgc
     3241 tcccaagcag ttaaatctcc tcgtgataaa cgttcagcaa tatcagcata aaattgtttc
     3301 ggtacttccc tttttacttg gtattgagct gattgttctt ctaataaatg ttcttctatc
     3361 atcttgattt cttctgttgt ccactcttca tcttctttat gtacaacaaa atcggtatct
     3421 acaatcacaa tagacatacc tagctccttc aagtactggt tatatattct gtattttgcc
     3481 tgttctaaat tcagctgtct agcttttctg ttactcacaa tttgcaaatt tttcatcaaa
     3541 ttccaccgct tgccattcaa aaaaaggcgt ttttgcggta tcctatcggc aattttttca
     3601 gttaaattta aaggttaaat tttatgcaac agcagtataa cccaagtgag attgaaccta
     3661 aggttcagcg ttattgggca gaaaataaag tttttaaagc agttaaagac gttaccaaag
     3721 agaaatacta ttgcctttca atgttgcctt atccgtcagg taagctacat atggggcacg
     3781 ttcgtaacta caccattggt gatgtagtgt ctcgttatca gcgtatgatc ggtaaaaatg
     3841 tattacagcc gatgggttgg gacgctttcg gtttaccggc ggaaggtgct gcggtgaaaa
     3901 ataataccgc accggcaaaa tggacttacg aaaacatcga atatatgaaa ggtcagctta
     3961 aaatgttggg cttctcttat gactgggatc gtgaagtcac cacttgccgt ccggaatact
     4021 acaaatggga gcaatggttc ttcacagaac tttataaaaa aggcttagtg tataaaaaaa
     4081 cctcaaccgt aaactggtgt ccgaatgatg aaactgtgct tgccaacgag caagttcacg
     4141 aaggttgttg ctggcgttgt gacaccccag tggaacagcg tgaaattcca caatggttta
     4201 tcaaaatcac tgactatgct gaagaattac tcaatcagtt agataatctc ccacagtggc
     4261 cggatcaagt aaaaaccatg caacgcaact ggatcggtcg ttccgaaggg gtagaaatca
     4321 cctttaaaat tgccggttca aacgtagaat taccggttta cactacccgt ccggatacct
     4381 tcttcggcgt aagctatgtg gcgattgctg cggctcaccc attagcagaa ttagctgcac
     4441 aaaataaccc ggcattggct gaatttatcc gtgaagcgaa aaacaccaaa gtggcagaag
     4501 cagaattagc caccatggag aaaaaaggaa tggcaaccgg cttgttcgcc attcacccgt
     4561 taacaggaaa agaagtaccg gtttgggttg ctaactttgt gttaatgcac tacggtacag
     4621 gtgcggtaat ggcggttccg gctcacgatg agcgtgactt tgaatttgca caaaaatacg
     4681 atttgcaaat taatcaggta attcaaccgc ttgatggctc agagtgggat ttcagcaaag
     4741 ccgcttacac cgaacacggt aaattaatca actctgccga gtttgacggc ttggaatttg
     4801 atgtggcttt cactgcgatt gccgataaat tagaggcaat gggcgtaggt aaacgccaag
     4861 tgaatttccg tctgcgtgac tggggtgttt ctcgtcaacg ttattggggt gcaccaatcc
     4921 caatgatgac cacagaagag ggcgaagtgg ttaccgttcc attacaagat ttaccggtga
     4981 ttctccctga agatgtggta atgaacggcg tgcaaagccc aatcaaagcc gacccagagt
     5041 gggcgaaaac cacttacaac ggcaaaccgg cattgcgtga aaccgatact ttcgatacct
     5101 ttatggagtc ctcttggtat tatgcacgtt atacttgccc acaatatcac gaaggaatgt
     5161 tagattcaga cgaggcaaac tactggctac cggtcgatca atatatcggc ggtattgaac
     5221 acgccacaat gcacttgctc tacttccgtt tcttccataa attgttgcgt gatgcgggta
     5281 ttttaaattc ggacgaaccg gcgactaaac tgttatgcca aggtatggtg cttgccgatg
     5341 cgttctacta caccagccca actaacgagc gtatttgggt cagcccgaca caagtcacct
     5401 tagaacgtga tgagaaaggt cgtatcatca aagcaaccga tccacaaagg cgtgagctgg
     5461 ttcacagcgg tatgactaaa atgtcgaaat caaagaacaa cggtatcgat ccgcaagaaa
     5521 tggtggaaaa atatggtgct gacacagttc gcttgtttat gatgtttgcc tcccctgcgg
     5581 aaatgacatt agaatggcaa gaatccggcg ttgaaggggc aaaacgtttc ttaggtcgtg
     5641 tttggaattt agtttatagc tatgcccaaa atccggcaac gactgcatta gatgtgaatg
     5701 ccttagacaa ggatcaaaaa gaacttcgcc gtgatgtaca caaaacgatt gctaaggtga
     5761 gtgatgatat tggtcgtcgt caaaccttca atacagcgat tgctgcggtg atggaattga
     5821 tgaacaagct caccaaagcc ccacttgaaa ccgaacaaga taaagccgta atggcagaag
     5881 ctttaagtgc ggtggtgcgt atgctttacc caatcacgcc acatatctgc tttgagttat
     5941 ggcaagtgtt aggtaatgaa caaaccatcg attttgcccc ttgggtacaa gccgatgaat
     6001 ccgcaatggt ggaagatgaa aaattagtgg tagtacaggt caacggaaaa gtgcgtggca
     6061 aaatcactgt ttcagctact gctacggaag atgaagttaa agctatcgct aaagccgatc
     6121 caaacgttgc taaattctta gatggtcagc agattgtaaa agaaatctac gttccattta
     6181 aaatgttgag ctttgcggtt aaggcgtaac aaacacactc tcccttatat gggagagaga
     6241 ccgataaata tttcgtgcag aaatatttat cagagagagg gtacaagcgg ttattttctt
     6301 ggaaaatttt gcaaaaaata gccccctctc cctgcttttt cttctcaacg aagaaaaacc
     6361 tgtccctctc ccacaagggg agaggggaat aaacaaggaa atctcattat gattaaaaaa
     6421 ctctctctca tcgctgtttt tgctcttacc gcctgcggtt ggcactttaa aaacaatgag
     6481 gtattacctg aatcgttcag aacattaacc tttgaaagtg cagatcaaca cagcgaaatg
     6541 tctcgaattt tacgcaatca gttacaacta agcgatgtaa aattagtgcc tagcacggct
     6601 aatgtggcaa aattacgttt ggtttcaacc tcaacaaaca gcaaagtggt ttctgtgttt
     6661 aaacaagctc gtgaagcaga aaaaatctta acgctaaacg ttgatgcgat tgccgatatt
     6721 ccaaataaag ggcaataccc tttagaagtt tcggttcatc gtactttctt tgatgactct
     6781 cgtgcagctt tggcaaagtc tgctgaaaaa gagatgatta tgaaagatat gtatgaacaa
     6841 gcctctcgcc aattaattat caaaatggcg ggcttaaaaa aagaactctc aaaataatca
     6901 ttgtaggggc gaaatatgtt tcgcccattg tttatctgta tgcaaaaaat ctttccggaa
     6961 acacttcaat tttcattaaa caaaggacta cagccctttt atctattaac agggaatgat
     7021 ctgctattgg tgaatgaaac caaagatgcc attattcaca ccgcccgtct caacggtttt
     7081 gatgaaaaaa aagaagttga aatcaaaaac gatactaact gggaagaact tttcgaagca
     7141 gctcaatcaa tgggattgtt ttttgcacgc caaattatgg tgctgaattt tccggattcc
     7201 cccacagcga cacagtttaa aaaactggct gagctttgtg cctttattca tagtgatttg
     7261 ctctttatcc tgcacttacc taaattttcc aaaacaatga aaaaacaggc ttggttcacg
     7321 caaattgagc taaatctcgt gcaaattaac tgccaaacac cggaacttag caaacttcca
     7381 caatgggtca gtcatcgagc aaaagcgatg aatttgcaaa ttgaaaatga agcaaaccaa
     7441 ctcctttgtt atagctacga aggcaatcta ctggcgctta aacaggcatt acaactgcta
     7501 caactcaatg caactgatgg aaaaatcacg ctgacaaaag cccaagaagt ggttgaacac
     7561 tccgcccaat ttactccatt tcaatggatt gatgcattat ttgaaggaaa aatccaaaga
     7621 gctcaacgca ttttgtacca tctgcaaaat gaagaagtac aagcggtcgt tttattaaga
     7681 attgtgcaaa aagagttgat gattttactc gaaatcactc gcaacccaac cgcagtcgtt
     7741 tccagcgaac aatcactttt tatcggtaat ttacgaacag aatttgaccg cttgaaaatt
     7801 tggcaaaacc gcagaccttt ttacaaccag atcactcaac gactgactta ccaaaaactt
     7861 tacgccctaa tccaacaact ggctgaatta gagcgtaagg tgaaacaaga atttagcgat
     7921 gatatttggc aagaattaga gcgtttttcc gctcaattta cctaactaat acttttctaa
     7981 atcttcgcta aacaactccg cttcttcact ggcgatagag tgcttttctg ccgcccaatc
     8041 gcctaaatca ataatttgac aacgctcgct acaaaacggg cggtatttac tttctttact
     8101 ccacaccact tctttttcac aagtcgggca gtttacaata gtttctggca ttctttttcc
     8161 ttttttgcta atgctaagta tttataatgt aaggcttgaa cctgctcttt aattaaaagc
     8221 aagccctgtt caaatggcaa gttattttca atcacatcat cagcataact taatctttct
     8281 tcacggctga cttgggctgc cataatattt ttaatggttt caattttact gttatcacgt
     8341 ttggttgctc tttccagttg gatttcagga gaaacatcaa caaccaatat acgatcacaa
     8401 tattcggtga gcttattttc aattaaaagc ggcacgaccc aaatcacata acaagcggtc
     8461 gattcttgca atttttttac catttcttct cgaatggcgg ggtgaagtaa attattgagc
     8521 cacgtttttt cctgttcatt gttaaacaca atttggcgaa gtttagtgcg ttctaatgct
     8581 ttttcagcgg ttaagatctc ttcaccaaaa tgctctacaa tcttggctaa aagcggtgta
     8641 cccggttcaa caacttagcg agccaccaca tcagcatcaa ttacctctac tccaagagct
     8701 gaaaataaat cagacactgt ggttttgcca ctgcctattc caccggttaa gcctacaacg
     8761 taagtcataa aaattgattc ctcaaaataa aaacaaacca attctagtaa ttaatccctc
     8821 tgctttcaag cattagcacc agcaacatcg ctgtgctaat aaacggaata aacgccaatt
     8881 ttgctaattt ctgctttttc attagccaat aagccaagta ataaccaatc cctagcaggc
     8941 tggcggttaa tatcaataag agcatttttt cgagccgaaa gagtggggat aacgcaacca
     9001 ataacaacat atcgcccgag ccaaatgcct cctttttaaa cataagctgt gaaaccaagc
     9061 caatcaacag gcaaaaagca agggtaaagg taaaagtttg gtgttggaaa tcggctaaac
     9121 tctcccataa caccaacaaa aatataagca cgaggtagcg aatatccgtt aggtaatagc
     9181 acatatccag cagtgataaa aaacagagca gaataaaaat cgtgccgata atagggcttt
     9241 ccgagcagag cataatcaat ggaaaaagga gaaaaaatag atttgccgat cggggttgtt
     9301 tcggttgtag caccgattga gccaaaaact gttgctcggt taagccaata ttcactaatg
     9361 aagttgtttc ttggtaaatt tggcgattga ctcgaatggc aaatcgaggg atctccgctt
     9421 tgagccaaac cgcaatgaag agggaaaaca gtattaacat cgctactcca ccaaagcccc
     9481 catatcaaaa atcggtaaat acagcccaac aataatagtg ccgacaatca cgcccattat
     9541 caacattagc atcggttcta atagctgaga caaaatatca atttggtaat ctaatttttg
     9601 ttggtacatc tcactaatat gctcacacat tttcgccaca ttgccacttt tctccccaac
     9661 ctcaagcatt tgtgccatct cagcaccaaa cacattcggg ttgatccctt gtgaaaattt
     9721 gtagccttgt tgtaacaaat taagcatagt gccaatttct tgctgtaaaa tcgggtcatc
     9781 atttttctcg gataaaaacg acctcaaggc aacatcaagc ggaatatgag cattcagcat
     9841 taaggcaata ttttggctga aaaagataat gcgagattgc tcaataattc ggctaaacag
     9901 tggcatttta gcccatagag ccattttcca ctttgcgact aacctgctct tttttgcaaa
     9961 aaacaggtaa aaaatgaccg ctagtagtaa cgtgccaagt aatactgtgc cttgctcaat
    10021 caagcattcg gaaagggtaa ataaaatatc ggtaatgagg ggtaaagttt tgtcttttga
    10081 gccataaagt tgggcaaact gcggtacaat caaaaccaat agcccgatag ataaagcgag
    10141 cgaaatcagc aacacaatca ccgggtagaa caggattttc ttcacttttt tcgccaattt
    10201 ttccaatttc gctcggcttt cagctaaatt agtcagcata atgcccagtt ttccgctttg
    10261 ttcgcccatt ttgattagct gaatttcctg attggtcaca aattcaccca atttctccaa
    10321 cgattgtgaa aaagcatagc cactttcaat atgctgaatc agctcattaa gccatagata
    10381 aaggcggata tttcggcagt tttgcaacaa catagataat gcctgtttta acggcatggc
    10441 agaattaacc aacaatgcaa gctgtgatag caagctattc acctcttctt ttttcgggtt
    10501 tgctgaaagc acaaaatttc ggctaatccg aatatgctga taaccttgtg ccagcaaaag
    10561 gttttccaac atgtctcgat tcgttgccaa ccgcttgccc cgctgttttt gcccaaatcg
    10621 gctctgagct tgccaatgaa attcatagat tttcgtcatt gcctagcacc cttaaaattt
    10681 ccgcctgatt ggtttttcct tccgtcaatt tttcttttgc actgtcggca agggttttaa
    10741 aatccaaaca agcggtgttt ttttcaaaat tttttgcaga tcggtttaag agttgatata
    10801 ccccaattcg ccctttatac ccttgataac aatgctcaca gcctgcccca ccgcatttca
    10861 cacacactcg gcgtaacagg cgttgagcaa tcacgagtag caatgaattt ttaagttcat
    10921 actcttttat gccgagctgt agcaaccgtt ctaccgctga aggggcatca ttggtgtgca
    10981 aagtcgaaag caccaaatgc ccggtttgtg ccgcccgtag tgccatttct gcactctctt
    11041 catcacgaat ttcgccgagc ataataatgt ccggatcttg gcgtagaaag gttcttaaca
    11101 gttggctgaa atctaagtta atccctcgat tcacttgggt ttggataatt ccctcaatct
    11161 caatttcaat cgggtcttcg gcagtcaaaa tatgcttttc actgctgttt aaatggctta
    11221 atgcactgta aagcgtgatg cttttaccgc ttcctgtcgg gccggttact aaaatcaatc
    11281 cttgaggctg actcaaagca ttcagcaaat tggctttctg agttgggtta aagcctaaat
    11341 ctaaaaaatc aaaatgggtt ggtttatttt tttgtagccg tagcaccact ttttcgccga
    11401 agtgcgttgg caaggtagaa acacggaaat ccagcgtttc agctaacagt gttttgaaat
    11461 gaaattgtcc gtcttgtggc aaacgagtct cgctgatatc gagcttagcc aataatttaa
    11521 cccgtgaaat caaacggctg gataaactac tcggcaggct tttataaata tgtagcacgc
    11581 catcaattct taaccgaatt aacaatctct ctttttgtgg ttcaagatgg atatcggaag
    11641 cgttctgttg caaacagaac ttaaacaaat tctctaaaat ttggatgatc ggatcatttc
    11701 tatccggctg ctcggtattc tcggaaaaag gataaagcgg attttcttca taaatttcac
    11761 tctgcgggct actctgttca ggcgaaaggg cattgagcaa aaattttaat tcttcggagg
    11821 aaatcaaaac aggctcaatc tgtttgtggt agataaatgc aaaaatctcg caggcattaa
    11881 gattttgcat atcatcaatt cccagccaaa gctgttctgc tgtttcttgc agtggaacgg
    11941 ctaagtaacg caacaaaatc tgcttttcat tacaattttt ctgccattgt tctgctgaaa
    12001 tctcgatgaa tctttggctg tttaagtcgg ttaccgaata ctccattttg atttcctcac
    12061 ctcaaattag ccgtttggtg taacagttgt gctacagaaa ttagctggaa ataggctcgt
    12121 atcattgcca ctacaacttg ccgtccaact aatcgtatta tttgagaacg acggtgttaa
    12181 ggtatagcca taaccattca gagagccttt gccggttacc gtaatcacgc cttttgttac
    12241 cgcaattgag tttaggtatt tagtggtatt actactggtt ttattcgcct gaatcccatt
    12301 cgtgccaccg gagcagttat ccagcccgcc actgttgtaa atacagagtt caacgtctga
    12361 tttatacgaa gcagaggctt gcaataactc cgataacgcc gccttttggg tgtatgagtt
    12421 ataggaaggg atcgcaatgg tggcaagaat ggcaataatc gcaatcacga tcatcaactc
    12481 aattagggta aatgcccgat gcagttggcg aatgccaagc ggtcgaaaat gtacaatttt
    12541 ttgcatataa atttccttat aaattgcacc aacaaattgt ggcgaatcaa gtgtgcaaat
    12601 tttttgccgg ctttgcgacc ggcaaatgtc aatttttcga tgcagatcga aaaaataaat
    12661 ttttcatctg tttat
//
LOCUS       DANKBV010000061        12659 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.061, whole genome shotgun sequence.
ACCESSION   DANKBV010000061 DANKBV010000000
VERSION     DANKBV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12659
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.061"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..476
                     /locus_tag="PWY43_001428"
     CDS             <1..476
                     /locus_tag="PWY43_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136302.1"
                     /translation="WYRQKDLSLTIEGMFIECLSLISCLLQPIFVLSIGINSENNISF
                     WEAIFLFWLTPLIILIITTYLIIFDNSKTINIALTLIIILLWICTVFNMTPLVMSFIG
                     VKEKIDKNYIINKQYYMTGKILLNQGEKFIFLEKGKLYNESIVIDKENIKLEDSN"
     gene            476..964
                     /locus_tag="PWY43_001429"
     CDS             476..964
                     /locus_tag="PWY43_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HDL2136303.1"
                     /translation="MNIKKHIFPLLLISNTAFAAPVELHQFDSPKQESDYRALIQELR
                     CPQCQNNNIADSNATIATDMRAKTLELLKQGKSKDEVVDYMIERYGNFVTYDPPLTPA
                     TIMLWLLPLILIALGLGVVLRRKKNKPQAVNFEQNTAKPTTHLAQDEQERLNQILNKE
                     EK"
     gene            961..1872
                     /gene="ccmI"
                     /locus_tag="PWY43_001430"
     CDS             961..1872
                     /gene="ccmI"
                     /locus_tag="PWY43_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HDL2136304.1"
                     /translation="MNFWLAIIFVTLIVCGVAFYPLLKKNKQIDNTKRDSLNKAFYFD
                     RLKEVENEANEGVIDDPEQTKLELQKNLLDDIPHSTQSATSEKLTLGKSWFVALILFV
                     GGISFAIYSNVGSWFSNSMLDTTHQKLDSFYERLKTEETNPLSQEEMNQFAMALRVDL
                     QSNPNDDKKWYMLGQIGMALDDGQLAHDAFSKAAQLKPDNLDYKLSHAQLLLFSEAQE
                     DKKQGETLLKEIIRQDHTNFNALSLLAFHSFEKEDFKMAAMTWGMMLKLLPDEDPRKV
                     TVERSFNMALSNLSEAEKQELQKGEKK"
     gene            1869..2474
                     /gene="mog"
                     /locus_tag="PWY43_001431"
     CDS             1869..2474
                     /gene="mog"
                     /locus_tag="PWY43_001431"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDL2136305.1"
                     /translation="MTACKPLSKEKLKIGLVSVSDRASQGVYSDQGIPELRAWLESAL
                     TEPFELETRLIPDEQAIIEQTLIELVDAHACHLVLTTGGTGPAKRDVTPDATLAVAHR
                     EMPGFGEQMRQVSLHFVPTAILSRQVGVIRHDSLILNLPGQPKAIKETLEGVKDEQGK
                     VLVKGIFSAVPYCLQLLSEIYVETHAAICESFRPKSARREN"
     gene            2484..2822
                     /gene="glnB"
                     /locus_tag="PWY43_001432"
     CDS             2484..2822
                     /gene="glnB"
                     /locus_tag="PWY43_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDL2136306.1"
                     /translation="MKKIEAIIKPFKLDDVREALTDVGVSGMSITEIKGFGRQKGHTE
                     LYRGAEYAIDFLPKVKVEIVIPDELEEQCIEAIMETAQTGKIGDGKIFVYDVGRVIRI
                     RTGEENEEAI"
     gene            2903..3382
                     /gene="lspA"
                     /locus_tag="PWY43_001433"
     CDS             2903..3382
                     /gene="lspA"
                     /locus_tag="PWY43_001433"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL2136307.1"
                     /translation="MKTGLKFLWVSLIVIIIDLWSKYIVVQHFEFGESINILPIFNFT
                     YARNYGAAFSFLADHSGWQKYFFLGLAVVISSALVVALWKNQAVKKLENIAYALIIGG
                     AIGNAIDRAYNGYVVDFLHFYWDIYHYPVFNIADIAICVGAGLLILEAFITKKEPKK"
     gene            3393..4337
                     /gene="ispH"
                     /locus_tag="PWY43_001434"
                     /gene_synonym="lytB"
     CDS             3393..4337
                     /gene="ispH"
                     /locus_tag="PWY43_001434"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDL2136308.1"
                     /translation="MKILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNKF
                     VVDGLKAKGAIFVEELHEVPDDAIVIFSAHGVSQVVRQEAKNRGLKVFDATCPLVTKV
                     HMQVARASKKGTKAILIGHEGHPEVIGTMGQYNNQEGGIYLVESVEDIANLPVSNNDE
                     LTFMTQTTLSIDDTIGVIEALKEKYPAIQGPRKNDICYATTNRQHAVRELAEQSQLVL
                     VVGSKNSSNSNRLAELASRMGVMSKLIDGPQDIDPTWLEGIETIGITAGASAPEVLVQ
                     SVISHLKTLGVTEVETLVGNEEDVVFEVPKELRIKEVK"
     gene            4337..4585
                     /locus_tag="PWY43_001435"
     CDS             4337..4585
                     /locus_tag="PWY43_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HDL2136309.1"
                     /translation="MTEINRFKLEWACRRGMRELDKMIMPFYQNHFNELNETQQQAFV
                     EMLNYSDPELFRWVMLQAPAPTEEIAELIRLMRDKIET"
     gene            4765..5097
                     /locus_tag="PWY43_001436"
     CDS             4765..5097
                     /locus_tag="PWY43_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF413 domain-containing protein"
                     /protein_id="HDL2136310.1"
                     /translation="MADSFSVTRRFFDDKNYPRGFSRHGDYTIRESQTLEQFGQACLA
                     LESGERKPATQEEKRFVAVMKGEEVAETDIEKAWLKYRTLTSKTKRIYTLSGNAGSDA
                     GDDFSAAE"
     gene            5162..6253
                     /gene="trmA"
                     /locus_tag="PWY43_001437"
     CDS             5162..6253
                     /gene="trmA"
                     /locus_tag="PWY43_001437"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDL2136311.1"
                     /translation="MTLPIEQYSDLLAKKTENLTALLKPFNPPAIEVFASKPSHFRMR
                     AEFRVWHEEGELYHIMFNPATKARYRVDSFPIASELINHMMTALLAEIKGNELLTRKL
                     FQIDYLSTLSGEIAVSMLYHKSLNEEWVEQANALKSRLEQQGFKVQIIGRANKQKIAL
                     DCDYVEEILPVHGKNYVYRQVENSFTQPNAEMNIKMLEWARNCTKNSEGDLLELYCGN
                     GNFSIALAENFRKVLATEISKSSVQSAQYNIEKNGIVNLQIIRMSAEEFTQAMNGVRA
                     FYRLKGIDLNAYECNTIFVDPPRAGLDQATLNMVQAYERILYISCNPETLAENLQQLS
                     QTHRIEKAALFDQFPYTHHIESGVWLIRK"
     gene            6345..6434
                     /locus_tag="PWY43_001438"
     tRNA            6345..6434
                     /locus_tag="PWY43_001438"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6379..6381,aa:Ser,seq:tga)
     gene            6590..6961
                     /locus_tag="PWY43_001439"
     CDS             6590..6961
                     /locus_tag="PWY43_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDL2136312.1"
                     /translation="MKYLYIICGFICIGLGIIGAVLPGLPTTPFLLLALFCFTKGSER
                     IRQWFIQTKLYKKYLQEYDEKRAMTMKQKITILAISFPFTLFAFFALPHIGGKIALVV
                     LVIIQYWYFFCKMKTLEPENA"
     gene            complement(6958..7869)
                     /gene="xerC"
                     /locus_tag="PWY43_001440"
     CDS             complement(6958..7869)
                     /gene="xerC"
                     /locus_tag="PWY43_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL2136313.1"
                     /translation="MTTSNPLFQQTRPYWDFLRIEKQVSPHTLSNYQRQLMAVSELFS
                     QAGIENWEEVESSSVRWMLSQSHKQGLSAKSIGLRLVALRQWFVYLIKIGRMSANPTL
                     GVKSPKVGKHLPKNIDAEQVGQLLAIESDEPSDIRDIAMMELMYSSGLRLSELQGLDL
                     GDMDLSAREVRVQGKGSKERIVPIGSQALKALNRWLEMRLQFKPQDNAVFLNKRGGRL
                     SHRSIQLAMKKWGERQGLESHLHPHKLRHSFATHMLEASGDLRAVQELLGHSSLSTTQ
                     IYTHLDFQHLAKIYDSAHPRARRKQED"
     gene            complement(7936..8226)
                     /locus_tag="PWY43_001441"
     CDS             complement(7936..8226)
                     /locus_tag="PWY43_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5377 domain-containing protein"
                     /protein_id="HDL2136314.1"
                     /translation="MSVKTEVLFSNHWNVRVSDPGFEGSPSHYFETVYLTLEAFINGE
                     NVHYEFTRKVEQEVKTHRSFTDVNELFEFLGEYLSPVALGNVGVRIGQLGLA"
     gene            8460..9128
                     /locus_tag="PWY43_001442"
     CDS             8460..9128
                     /locus_tag="PWY43_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL2136315.1"
                     /translation="MPDKLEYLHDLDKEIMLSYFSLSEAIAGLLGEFCEVVIHSLNAY
                     EKSVIKIINGHHTNRKIGSPITDKGLQLLKCYQKDQSNTELVYFSRLNNGNLVKSTTH
                     IIIGHEGKPIGLFCINLNLSTPLDKTVSSFMNYDPIPPHYKSENFITHPKELIEETFL
                     KAVTHIEQHTTKHSKLYKKSIIFYLFENGIFELKEAVPIVAEKLSLTSHAIYKHLREF
                     KNNT"
     gene            9157..10435
                     /locus_tag="PWY43_001443"
                     /pseudo
     CDS             9157..10435
                     /locus_tag="PWY43_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249977.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10449..11750
                     /locus_tag="PWY43_001444"
     CDS             10449..11750
                     /locus_tag="PWY43_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDL2136316.1"
                     /translation="MNIHCEKLVQSILHAVNHEVVPALGCTEPISLALASAIARQYLG
                     HLPERIEAKVSPNLMKNGMGVAVPGTATVGLPMAAAIGAIGGEPDGGLEVLKNITPEQ
                     VKQAKAMLNTDKVSVSIFETEHILYSEATLFHQANRVCVRIAEHHTNVIYIEKNGQTI
                     LSKPCAVNNDNTTEIFASLSAKDIFNFSMQIELEKIRFIEQAAQLNSALSQEGLRADY
                     GLHIGRTLQKQIGLGLISDDLLNRIVIETTSASDARMGGASLPAMSNSGSGNQGITAT
                     MPIVVVARHLNVTDEQRIRALFLSHLMAIYIHSKLPKLSALCAVTTAAMGSCAGIAWL
                     LTGKFENISMAISSMIGDISGIICDGASNSCAMKVSTSVTSSYKSILMSMDNSQVTGN
                     EGIVEHDIDRSINNLCSIASRSMQYTDRQVIEIMVNKPKLK"
     gene            complement(11855..>12659)
                     /gene="glyS"
                     /locus_tag="PWY43_001445"
     CDS             complement(11855..>12659)
                     /gene="glyS"
                     /locus_tag="PWY43_001445"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2136317.1"
                     /translation="EDEEVAVALNEQYMPRFAGDNLPNSLVACSVALADKFDTLTGIF
                     GIGQAPKGSADPFALRRAALGSLRIIVEKNLPLDLTDLVAKSAQLFGDKLTNKDVVED
                     VVDFMLGRFRAWYESEGIAVDVIQAVLARRPTKPADFDARVRAVSHFRTLDSAEALAA
                     ANKRVSNILAKADIAIGDIDVSACVEPAEKALAEAVIALKAEVQPLIAQGDYTAVLDK
                     LANLRQPVDAFFDGVMVNAEDQKLRQNRLAILSTLQGLFLQVADISLLQ"
BASE COUNT         3834 a         2642 c         2564 g         3619 t
ORIGIN      
        1 ggtggtatag acaaaaagat ttatccttaa caattgaggg gatgtttata gaatgcctat
       61 ctttaattag ttgtctattg caacctatat ttgtactatc tataggaatt aatagtgaaa
      121 ataacatctc tttttgggaa gctatctttt tattctggct tactccttta attatactaa
      181 tcataactac atacctgatc atatttgaca attcaaagac aatcaacata gcccttacct
      241 taattatcat cttactatgg atttgtactg tttttaacat gacccctctt gtaatgtctt
      301 ttataggagt aaaagaaaaa attgataaaa attatataat taataaacaa tattatatga
      361 caggtaagat tttattaaac caaggagaaa aatttatttt cttagaaaaa gggaaattat
      421 ataatgaatc aatagttata gataaagaaa acattaaact agaggactct aattaatgaa
      481 tataaaaaaa cacattttcc ctttactatt aattagcaat accgcctttg ctgcccctgt
      541 tgagcttcac caattcgaca gcccgaaaca agagtcggac tatcgtgcct taattcaaga
      601 actgcgttgc ccgcagtgtc aaaataataa tatcgccgat tccaatgcga ctattgccac
      661 cgatatgcgg gcaaaaacct tagaattgct caaacaaggc aaaagcaaag acgaagtggt
      721 ggattatatg attgaacgct acggcaattt cgtcacttac gacccgcctc tcacaccggc
      781 aaccattatg ttatggctgt tgccgttaat tttaattgcc ttagggctgg gggtggtgtt
      841 gcgtagaaag aaaaacaaac cacaagcggt caattttgag caaaatactg caaaaccgac
      901 cactcatctc gctcaagatg agcaagaacg tcttaaccaa attctcaata aggaagaaaa
      961 atgaatttct ggctggcaat tattttcgtt acactgattg tatgtggcgt ggcgttttat
     1021 ccgttactta aaaagaacaa gcaaatcgat aacaccaagc gagacagctt aaacaaagcc
     1081 ttctattttg accgcttaaa agaagtggaa aacgaagcga atgaaggggt gattgatgac
     1141 cctgaacaaa ccaaattaga gctacaaaaa aacttgcttg atgatattcc acacagcaca
     1201 caaagtgcca caagtgaaaa attaacgctc ggcaagtcgt ggtttgtggc attgatttta
     1261 tttgtcggcg gtatcagctt tgcgatttac agcaatgtcg gttcttggtt ctccaactca
     1321 atgttagaca ccactcacca aaaattagac agcttctatg agcgtttaaa aaccgaagag
     1381 accaatccgc tctcacaaga ggaaatgaac caatttgcga tggcattacg ggtagattta
     1441 caaagtaatc cgaatgacga caaaaaatgg tatatgctcg gtcaaatcgg gatggcgtta
     1501 gatgatggtc agcttgcaca cgatgccttt agcaaagcgg ctcaactcaa gccggataac
     1561 ttagattaca aactcagcca cgcccaactg ctgctctttt ccgaagcaca ggaagacaaa
     1621 aaacagggtg aaaccttgtt aaaagagatt atccgccaag accacacaaa ctttaatgcg
     1681 ttaagtttat tggcgttcca ctcctttgaa aaagaagatt tcaaaatggc ggcaatgacg
     1741 tggggaatga tgttaaaact actgcctgat gaagatcctc gcaaagtaac ggtagaacgc
     1801 agttttaata tggcgttatc caatttaagt gaggcggaaa aacaagaatt gcaaaaaggt
     1861 gagaaaaaat gaccgcttgt aaaccactta gcaaagagaa gttgaaaatc ggcttggtgt
     1921 cggtttcaga ccgagcctcg cagggggttt attcagacca aggcattccc gaattaaggg
     1981 cgtggcttga atctgcctta accgagccgt ttgagctgga aacccgctta attcccgatg
     2041 agcaagccat tattgagcaa accttaatcg agttagtcga tgctcacgct tgccatttgg
     2101 tattaaccac cggcggcacc ggcccggcaa aacgtgatgt caccccagat gccacccttg
     2161 ctgtcgccca ccgagaaatg cccggctttg gcgaacagat gcgacaagtg agccttcact
     2221 tcgtgccaac cgccattctc tctcgccaag tgggagtaat tcgccacgac agtctgattt
     2281 taaatctgcc ggggcaaccg aaagcaatta aagaaacgct ggaaggtgtg aaagatgaac
     2341 agggcaaagt attggttaaa ggtattttta gtgccgtgcc atactgttta caacttttaa
     2401 gcgaaattta tgtcgaaacc cacgcagcca tttgcgaaag tttcagaccc aaatcggcaa
     2461 gacgagaaaa ttaaggctaa aaaatgaaaa aaatagaagc aattattaaa ccttttaaat
     2521 tagacgatgt gcgtgaagcc ttaaccgatg tgggtgtcag cggaatgagt attaccgaaa
     2581 tcaaagggtt tggcagacaa aaaggacata ccgagcttta tcgtggggcg gaatatgcga
     2641 ttgattttct accgaaagtg aaagtcgaaa tcgtgattcc cgacgagttg gaagaacagt
     2701 gcattgaagc aattatggaa accgcccaaa ccggcaaaat cggcgatggc aagatttttg
     2761 tgtatgatgt cgggcgggtg atccgcattc gtaccggcga agaaaatgaa gaggcgattt
     2821 agttttgcaa aaaatccggc aaaaacgacc gcttgcaggg ggatttccat ctactctttt
     2881 tacattaaat tttggatatt aaatgaaaac aggcttaaaa tttctttggg tgagtttaat
     2941 tgtaatcatc attgaccttt ggtcaaaata cattgtagtg caacattttg aatttggtga
     3001 aagcattaat attttgccga tttttaactt cacttacgcc cgaaactacg gggctgcctt
     3061 tagtttcctt gccgaccatt ccggctggca aaaatatttc tttttagggc tggcggtagt
     3121 gatttccagt gctttagtgg tggctctttg gaagaatcaa gcggtcaaaa aattagaaaa
     3181 tattgcgtat gccttaatta ttggtggagc gattgggaat gccattgacc gtgcttacaa
     3241 cggctatgta gttgatttcc ttcattttta ttgggatatt tatcactacc ctgtgtttaa
     3301 tattgccgat attgcgattt gtgtgggtgc aggcttactg attttagaag cctttataac
     3361 caaaaaagaa ccgaaaaaat aagagtgatt aaatgaaaat tttattagca aacccacgcg
     3421 gtttttgtgc cggcgtggat cgagccattt cgattgttga attagcctta gaaattcacg
     3481 gtgcacctat ctatgttcgc cacgaagtgg tacataacaa atttgtggtg gatggcttga
     3541 aagccaaagg ggctattttt gttgaggagc tacacgaagt gccggacgat gctatcgtga
     3601 tcttctcagc ccacggggtg tcgcaagtag ttcgccaaga agccaaaaat cgtggcttaa
     3661 aagtgtttga tgccacttgc ccactcgtga ccaaagtgca tatgcaggtt gcccgagcca
     3721 gtaaaaaagg gacaaaagca attttaatcg gtcacgaagg tcaccctgaa gttatcggta
     3781 caatgggaca atacaataac caagaaggtg gcatttattt ggtagaatcg gtggaagata
     3841 ttgccaactt gccggtttcc aacaacgatg aattaacttt tatgacgcaa accacacttt
     3901 ccattgacga taccattggc gtgattgagg cgttaaaaga gaaatatccg gcaattcaag
     3961 gccctcgcaa aaatgatatt tgttatgcta ccaccaatcg ccaacacgca gtgcgggaac
     4021 tggcagaaca atcacaatta gtgttagtgg taggttcaaa aaactcctcg aactcaaatc
     4081 gtttggcgga actcgcctct cgaatgggtg tgatgtcaaa actgattgat ggtccgcaag
     4141 atattgatcc aacttggttg gagggtattg aaactatcgg tattaccgcc ggtgcttccg
     4201 ctcctgaagt gttggtgcaa tcagtaattt cgcatttaaa aacccttggc gtaaccgaag
     4261 ttgaaacctt agtcggcaac gaagaagacg tggtgtttga agtcccgaaa gagttacgca
     4321 tcaaagaggt aaaataatga cagaaatcaa ccgctttaaa ttagaatggg cgtgccgacg
     4381 tggtatgcgt gagttagaca aaatgattat gccgttttac caaaatcatt ttaatgaact
     4441 caatgaaacc caacagcaag ctttcgttga aatgctcaat tacagcgacc cggaactgtt
     4501 tcgctgggta atgctgcaag cacctgcccc aaccgaagaa atcgctgaat tgattaggtt
     4561 aatgcgagat aaaatcgaaa catagcccca ccgcttgcaa cttgcaagcg gtgatttttt
     4621 gcataaactt tgcatattgg gcgttaatgc aaaaaattcg ctaaatttga ccgctttttt
     4681 acattaaggc attgatttat tcgctgtttt tgtttaatgt atgccgattt tattttccct
     4741 aatttcaaac aacgaggcat tattatggca gacagtttca gcgttacgcg ccgctttttt
     4801 gacgataaaa attatccacg tggtttctct cgccacggcg attacaccat tcgtgaatca
     4861 caaactttag aacaattcgg tcaagcttgc ttggctttag aaagtggtga acgtaagccg
     4921 gcaactcaag aagaaaaacg tttcgttgcg gtaatgaaag gcgaagaggt agcagaaact
     4981 gacattgaaa aagcgtggtt aaaataccgc acattaacta gtaaaacaaa acgcatctac
     5041 accctttcag gcaatgcagg tagcgatgcc ggcgatgact tcagtgctgc tgaataattt
     5101 ctaatttaaa accacgaagt aattcgtggt ttgttttttc atttgctatt tgtggtaaaa
     5161 aatgactctt cctatcgaac aatattccga tcttcttgca aaaaaaaccg aaaatttgac
     5221 cgctttactc aagccgttta acccgcctgc gattgaggta tttgcctcaa agccaagcca
     5281 tttccgtatg cgggcagaat ttcgagtgtg gcacgaggaa ggcgagctgt accacattat
     5341 gttcaaccct gcaaccaaag ctcgttatcg ggtggatagc ttccctattg ccagtgagct
     5401 gattaaccat atgatgaccg cattattggc ggaaattaaa ggcaatgaac tactgactcg
     5461 caagctgttc cagatcgatt atttaagcac cttaagcggt gaaattgcag tctcaatgct
     5521 ttaccacaaa agcctgaatg aagaatgggt tgaacaggca aacgccttaa aaagccgttt
     5581 agagcaacaa ggtttcaaag tgcaaattat cgggcgtgcc aataaacaaa aaatcgcact
     5641 ggattgcgat tatgtggaag aaattctacc ggtacacggc aaaaattatg tgtatcgcca
     5701 agtggaaaac agctttaccc agccgaatgc ggaaatgaat attaaaatgc tggaatgggc
     5761 gagaaattgc acaaaaaata gtgaaggcga tttgcttgag ctttactgcg gcaacggcaa
     5821 tttttcgatt gccttggcgg aaaacttccg caaagtgctg gcaaccgaga tctcaaaatc
     5881 ttcagtgcaa tcggctcaat acaatattga aaaaaatggc attgttaacc tacaaattat
     5941 ccgaatgtcg gcagaagaat ttacccaagc gatgaacggt gtgcgagcct tttatcgctt
     6001 aaaagggatt gatttaaacg cctacgaatg caataccatt tttgttgatc cacctcgtgc
     6061 agggttagat caagctacat taaatatggt gcaagcgtat gagcggattc tctatatctc
     6121 ttgcaatccg gaaacccttg ccgagaattt acaacaatta agccaaaccc atcggattga
     6181 aaaagccgct ctttttgacc aattccctta cacccaccat attgaaagtg gcgtttggtt
     6241 aattagaaaa taaattgtgc tgaaaaaaag aacgaataag cagcttttaa gatttattac
     6301 ttgacgatgt tcgcaataat ctctattatt gcaacatttt caacggagga atggcagagc
     6361 ggttgaatgc accggtcttg aaaaccggcg agggttaacg ccctccgtga gttcgaatct
     6421 cacttcctcc gccagtcttt aaacctgttg agcaatcagc aggttttttt atttcatttg
     6481 caagaaaatc agataaaacc accgcttgaa tccatcgcta taaactggtt gaaatcagcc
     6541 taaagttgct ttaggataat tgcgattcat tatcaataac ttaattatta tgaaatacct
     6601 ttatattatc tgtggtttta tttgtattgg tttaggcatc atcggggcag tattaccggg
     6661 cttgccgacc acaccgttct tacttttggc tctgttctgc tttactaaag gctcggagag
     6721 aatccgccag tggtttattc aaaccaaatt atacaaaaaa tatctgcaag aatatgatga
     6781 gaaacgggca atgacgatga agcaaaaaat cacgatttta gcgatttcgt ttccgtttac
     6841 actatttgct ttctttgcac tgccccatat cggtgggaaa attgcgttag tagtgttagt
     6901 gataattcaa tattggtatt tcttctgcaa aatgaaaacc cttgaaccgg agaatgccta
     6961 atcttcctgc ttccgccttg ctcttgggtg ggcagagtca taaattttag ctaagtgttg
     7021 gaaatccaga tgcgtataaa tttgagtggt ggacaggcta ctatgaccca ataattcctg
     7081 aaccgctcgc aaatcaccgc ttgcctctaa catatgagtc gcaaaagagt gacgtaattt
     7141 atgtggatgc agatggcttt ccagcccttg tcgctcaccc catttcttca ttgccaactg
     7201 aatggaacga tgagataacc gcccacctcg cttattcaga aacaccgcat tatcttgcgg
     7261 cttaaattgc aaacgcattt ctaaccagcg atttaacgct tttaacgctt gcgagccaat
     7321 cggcacaatt cgctctttgc tacccttacc ttgcacccgt acttctcgtg ccgataaatc
     7381 catatcgcct aaatccagcc cttgtaattc cgataaacgc aagccggaac tatacatcag
     7441 ctccatcatc gcaatatctc gaatatcgct cggctcatca ctctcaatcg ccagcaactg
     7501 cccgacctgc tccgcatcaa tatttttcgg caaatgctta cctactttcg gcgatttcac
     7561 ccctaaagtc gggtttgccg acattcgccc gattttaatc aaatagacaa accattgccg
     7621 taacgccacc aagcgtaagc cgatactttt cgcactcaac ccttgcttat ggctttggct
     7681 taacatccaa cgcaccgagc tactttcgac ctcttcccaa ttttcaatgc ctgcttgcga
     7741 aaaaagctcg ctcaccgcca ttaattggcg ttgataattg cttaaggtat gcgggctaac
     7801 ttgcttttca atgcgtaaga aatcccaata ggggcgggtt tgttggaaaa gagggttgga
     7861 agtggtcata aacgtaataa caagcggtta atttttagga aaatgttgca agggcaaaag
     7921 atttgccctt tgagattagg ctagccccaa ctgaccgata cgcacgccaa cattaccaag
     7981 tgcaacaggg cttaaatatt cgccgagaaa ttcaaacaat tcattcacat cagtaaaact
     8041 gcgatgagtt ttcacttctt gctccacttt acgagtaaat tcataatgta cattttcgcc
     8101 gttaataaag gcttccagcg ttaaatatac ggtttcaaaa tagtggcttg gcgagccttc
     8161 aaagcccgga tcactcactc gaacattcca atgattacta aataaaactt cggtttttac
     8221 tgacattgtg ttttccttct tcctataagt acgtacatta ttttaatcaa gatagattga
     8281 atttgccact tttcctagtc agaatcgagg caaatcttat ttggtttgtc ctcttatatc
     8341 tttccctcaa aatatgatct tggtcaaaaa atactgactt ttaactctta ataaagttaa
     8401 atataattga ctttaaaatt tactcattct tataattcac aaaaattgga gtaaaagtta
     8461 tgccagataa attagagtat cttcatgatt tagacaaaga aattatgctg tcttatttct
     8521 cattaagtga agcaatagca gggttattag gagaattctg tgaggtggtt attcattcat
     8581 tgaatgccta tgaaaaatcc gttattaaaa tcattaacgg acatcatact aaccgaaaaa
     8641 taggttcgcc tattacagat aaaggattac agttactaaa atgttaccaa aaagatcaat
     8701 ctaatactga gctggtttac ttttcacggt taaataacgg taacttagtt aagtccacca
     8761 cgcatattat tatcgggcat gagggcaaac ctatcggatt attttgtatt aatttaaatt
     8821 tatcgacacc attagataaa accgttagct cttttatgaa ttacgatcct attccgcctc
     8881 attataaaag tgaaaacttt attactcacc caaaagaact tattgaagaa acctttctaa
     8941 aagcagttac tcacattgag caacacacta ccaaacattc aaaactctat aagaaatcca
     9001 ttatcttcta tttgtttgaa aatggcatct ttgagctaaa agaggcagta cctattgtgg
     9061 cagaaaaact ctccctaacc agccacgcaa tttataaaca tttaagagaa ttcaaaaata
     9121 acacttaatt atactgttac gatttaagga gactctatgg atactcaaaa actctcgcct
     9181 gccgaattta aacaagcaac aaaatttgat tctaccgact tcggctggat cattatgagt
     9241 attggtatgg ctatcggggc aggcatcgtc tttttaccgg tacaagtagg acttatgggc
     9301 atgtgggttt ttctactttc ttctattatc ggatacccgg cgatgtacct ttttcagcgt
     9361 ctgttcatta atactttagc agaatctccg gaatgcaaag attaccccac cgttattagt
     9421 ggctacctgg gcaaaaactg gggaatcttt ttaggtgtac tttattttat tatgttagtg
     9481 atttggatgt ttgtttattc taccgcaatt acaaacgaca gtgcctccta tcttcttacc
     9541 ttcggcatta ccaatacact actctctgat aatccatttt atggcttagt gctaatctgt
     9601 atattagttg ccttatcttc tcgaggcgaa aagctactgt ttaaaatatc tacatttatg
     9661 gtattaacta agctatttgt cgttgccgcc ttagggcttg caatgattgg aatgtggcaa
     9721 ctccataata tcggtgcaat gcctcctttc ggcaaactta taaaagatgc catcattacc
     9781 cttcctttca ccctaacctc tatactcttc ttgcaaactc tcagcccaat ggtaatttct
     9841 taccgtgcta aagaacagtc tcgtgaagtt gcgcgctata aagccctacg agcgatgaat
     9901 attgcatttg ctatcttatt tattaccgtt ttcttttaag ctgtctcttt tacacttgca
     9961 atggggagag aagaagcgct caaagcatac gagcaaaatg tttccgcatt agcaattgca
    10021 gcccaatttt tccctggtag ttgggcaacc tatgtaggtg ttgcattaaa tatttttgcc
    10081 gttatgacag cctttttcgg ggtatatttt aggttttaga gaagccactc aaggcattgt
    10141 gatgaaccta ttattaagaa aattcccggc tgaaaaaatc aatcctaatt atgtgcagaa
    10201 agggattatg ttattttcca ttctgctggc ttggagtgcg attgtattaa atgcaccggt
    10261 tttaagcttt acctcaatct gtagtcctat ttttggctta gttgggtgct tgattccggc
    10321 ctatttagtc tataaagtgc ctgcattatt caaatataaa gggctagcac tcaatattat
    10381 tatcattacc ggcattttat tatgtatttc tccattcctc acatttttag agtaataagg
    10441 ttttatctat gaatattcac tgcgaaaaat tagtacaatc cattttacat gcagtcaacc
    10501 atgaagttgt ccctgcatta ggctgtacag aacctatttc tctcgccctt gcatccgcca
    10561 ttgcacgcca atatttaggt catttaccag aacgtattga ggcaaaagtt tcccctaact
    10621 tgatgaaaaa tgggatggga gtagcagtac cgggaactgc aaccgtcgga ttgcctatgg
    10681 cggcagctat tggggcaatc ggaggggagc ctgatggtgg attagaagta ttaaaaaata
    10741 ttacgccaga acaggttaag caagctaaag ctatgttaaa taccgataaa gtgtctgtct
    10801 ctattttcga aacagaacat attctatatt ctgaagcaac actatttcac caagcgaatc
    10861 gagtatgtgt tcgtattgct gaacaccata ccaatgtcat ttacattgaa aaaaacggac
    10921 aaactatttt aagcaaacct tgtgccgtca ataacgataa tactactgag atctttgctt
    10981 cactttcggc aaaagatata tttaattttt ctatgcaaat cgaattggaa aaaattcgct
    11041 ttattgagca agcagcacaa ttaaattccg cactctcaca agaaggatta cgtgccgatt
    11101 atgggctaca tatcggaagg acattgcaaa aacagattgg tctaggtcta atcagtgatg
    11161 atttgcttaa tcgcattgtt atagaaacca cctctgcctc tgatgctaga atgggcggtg
    11221 caagtttacc tgctatgagc aattcgggtt caggtaatca aggaattaca gcaactatgc
    11281 cgattgttgt cgttgcccgc catttaaatg tcactgatga gcaacgcatt agagcactct
    11341 ttttatcaca cctcatggca atttatattc acagcaagtt accgaaatta tctgcactct
    11401 gtgccgtaac aaccgcggct atgggaagct gtgccggcat agcgtggctg ctcacaggga
    11461 aatttgagaa tatcagtatg gcaattagta gtatgatcgg cgatattagc ggtattatct
    11521 gtgatggtgc ttcaaacagt tgtgcgatga aagtctccac cagcgtaaca tcaagctata
    11581 aatccattct gatgtctatg gataatagcc aagtaacagg caacgaagga attgtcgaac
    11641 acgatattga tcgttcaatt aataacttat gcagcattgc ctctcgtagt atgcaataca
    11701 cagaccgcca agtcatcgag attatggtga ataaaccaaa acttaaataa tcccgccttt
    11761 ggtaaaattt caagcaaaaa agaccgcttg cagatcacaa ttactgcaag cggtctattt
    11821 tcattaaaat tttgcaatta ttgcaaactt attgttattg caacagcgaa atatccgcca
    11881 cttgcaggaa caagccttgt agcgtgctta aaatggcgag gcggttttga cgcagttttt
    11941 ggtcttctgc atttaccatt actccgtcaa agaaggcatc aaccggttgg cggaggttcg
    12001 ctaatttatc cagcacagcg gtgtagtcgc cctgtgcaat cagcggttga acttcggctt
    12061 tcaacgcaat aaccgcctcg gctaatgctt tttctgctgg ctctacgcaa gctgaaacat
    12121 caatgtcgcc aattgcaata tcggctttgg caagaatgtt actcacccgt ttattcgctg
    12181 cggctaaggc ttcggcactg tcaagggtgc ggaagtgaga cacggcacgc acacgggcat
    12241 cgaagtcggc aggcttggtc ggacggcggg cgagaacggc ttggatcacg tccaccgcaa
    12301 tgccttcgct ctcataccac gcacggaagc ggccgagcat aaagtccacc acgtcctcaa
    12361 ccacgtcttt gttggtgagt ttatcgccaa ataattgagc agatttcgcc actaaatcgg
    12421 ttaaatcaag cggtaaattt ttctccacaa taatccgcaa cgagccaagt gccgcacggc
    12481 gtagggcgaa cgggtcggca ctgcctttcg gggcttgacc aatgccgaaa atccctgtta
    12541 gcgtgtcaaa tttgtccgcc aacgccaccg agcaagccac caatgaattt ggcaagttat
    12601 cgccagcaaa acgtggcata tactgctcgt ttaacgccac cgccacttct tcatcttca
//
LOCUS       DANKBV010000062        12601 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.062, whole genome shotgun sequence.
ACCESSION   DANKBV010000062 DANKBV010000000
VERSION     DANKBV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12601
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.062"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..2453
                     /gene="hrpA"
                     /locus_tag="PWY43_001446"
     CDS             <1..2453
                     /gene="hrpA"
                     /locus_tag="PWY43_001446"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDL2136318.1"
                     /translation="FRLTPIGRQLAQLPVDPRLGRMVIEAAKNGSLHEVMMIVSALSI
                     QDPRERPQEKQQSADDKHRRFADKESDFLAFVNLWHFIQAQQKELSKNQFRNLCRKDY
                     LNYNRVREWQDIYHQLRLAVREMGLKINSEPASYQPIHTALLAGLLSHIGLKDNEKMH
                     YLGARNAHFFIFPNSALFKKQPKWVMAAELVETTKLWGRMVARIEPEWVEPLAVHLTK
                     SSYSEPHWAKSKGAVMASEKVSLYGIPIVASRPVNYGSIDPAVSREVFIRSALVEGEW
                     HNNYKFFKENHRLIREVEALEHKSRRRDILVDEQVLFEFYDQRIGTEVVSSKHFDTWW
                     KQASKKDPELLNFEKSFLINDNANLASELDFPNFWHQGDLKFKLSYQFEVGKDHDGVT
                     VHIPLSLLNQVEPEGFDWQISGLRHELIVSLIKSLPKSLRRNFVPAPNYADAFLGRAE
                     PFKKSLLDSLSHELRRMTGVLVEPEQWDLSQLPPHLRMTFRVIDDKGKKLQESENLDE
                     LKFALKDEVQNTLSNIADDGIEQNGVHIWNFAELPQFYEQKKAHFSVKAYPAIVDEQT
                     AVGVKLFETEFEQARAMQAGLRRLLLLNVPSPIKYLHEKLPNKAKLGLYFAPFGKVLE
                     LIDDCIACAVDKLVEEFGGFVWTEEKFTQLHEFVRANLNDKTVEIAKQVEKCLTLAYE
                     LNKRLKGKLDFTMAFALSDIKTQIEGLIYPDFVTKTGYARLADLYRYLTAIERRLDKL
                     LVDSNTDRAKMLRVEAVQEAYKQLLAKLPKSKAVPDEVLEIRYMIEELRVSLFAQQLG
                     TKYPISDKRILNVINEIK"
     gene            2703..5513
                     /locus_tag="PWY43_001447"
     CDS             2703..5513
                     /locus_tag="PWY43_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL2136319.1"
                     /translation="MYKIKHSFNKTLIAISISSFLSIAYATESIENPQPIIQLSESIS
                     TKYSGKGVKLGILDEGFMLKHPRHSQNIHPITHQLTTPDGEVRTYDPSYIQFEVKPFE
                     QNGETAFATLFETHGAGVAGIISAKADKTIGNGYSGGVAKGVELYVTSKSYRRTLEEV
                     LRDVQKELENKDNEEEEEEKKPNLAELGKADLFVSKEKEVVFERSEWATGLNKLLDKK
                     VFAINNSWNPDPISDDIHEIDKFYQSITQNKQNPLLQAIMRAKDNDTLLVFANGNESK
                     KQPGIMTLLPRYFSELEKHLIAVAAVDNKQQITEYSNHCGASKNWCVSAPGNLHLLNA
                     APDAKKNPQYGLSKDEGTSFATPVVTGSLAVLKERFDYLTATQIRDTLLTTSTDLGEK
                     GVDSVYGWGLINLKKAINGPTQFLNDETVTVTRDDHWSNPLASQFKITKKGDKSLHLD
                     GENHLDTVAVEEGRLALNGKTKVKTISNHANLAVNGTEVEQNYSSSGQSQLEVLGKSG
                     LIANAQANIHLAGSLKIDDKLTEKTEAGDVSATIVQLKDKATYQGGFTQLVENENLAK
                     RGLIQDLYFKDSEIIAKVNKPLTDEKADTNGQAGLALLNALRTTPIAYRRGWYNDWLQ
                     SALEQRKLDNLHYAVSNNIYADSLELLRSQNAKGLTQTQQHLFTAYHTPQQTAVWAEH
                     LNQKQSASSKHTDVKHYQSLLGVSHKLADKTGLSATLSQQKNRLAKPFSQATLKQTAL
                     NIGLRYHLDDAWFSEATLQFARQKYQQSRRFASHQLGTAETRGSTLGGEMRIGYQFMP
                     NQWIIEPSLGVQWIQTKMNGLNESGELATQTAAMRYRDVNIVPSVKLQRTFQLEQGSI
                     SPYIGLNYLHRLSGKTTKITSDIAGKTLHSEATVKRNRQLNGEIGVKLHYKNWFTTMN
                     LDYSRVKSANLFGWKASIGLTF"
     gene            5606..6484
                     /locus_tag="PWY43_001448"
     CDS             5606..6484
                     /locus_tag="PWY43_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDL2136320.1"
                     /translation="MNNSFHFPTFIEIFPKEKRRFKLLREYLRYSWHKAWCYAECKEF
                     TNYLNYSPLWKKLFNQNLYRVNALLGTYCDKKFNKRQRLNIIMENFAYAEKLFGLAYC
                     NKLVEQKNLCLLELSDELKLYLSINNIDPLEGFFSLNIQNNEGIHIYDASFTFLASNK
                     LLVSSIQGPNTASAQDLIKSATKQLHGVRPMFMLINVFKFIAQHFDLELLGIPHKRQA
                     KYRWNDHSKLLFNYDAFWQENGGVITPDKYWHLPTSLDIRELNTIPSKKRSMYKKRYD
                     LFNKIEKVIKSLDKGQ"
     gene            complement(6589..7026)
                     /locus_tag="PWY43_001449"
     CDS             complement(6589..7026)
                     /locus_tag="PWY43_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL2136321.1"
                     /translation="MDWFFNWIGWSILGFVLLALELVLPGVFIMWWGFASLILAFIVA
                     LFTLGQALQISLFAIIATIFSLIWWKYQHGKDKKEDQSSSLNSREHAMLGAQGFVVEI
                     LPNGIVRGKFGDTTWRVIGNNLQVADKIEVQKVEGITLFVRKQ"
     gene            complement(7062..7982)
                     /locus_tag="PWY43_001450"
     CDS             complement(7062..7982)
                     /locus_tag="PWY43_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDL2136322.1"
                     /translation="MNFDLPIVSIAFVVLVLVALSSTIKIVPQGFHWTVERFGRYTKT
                     LSPGLNIVVPFIDRIGRKMNMMEQVLDIPSQEVISRDNASVAIDAVCFVQTVDARRAA
                     YEVNHLEQAIVNLTMTNMRTVLGSMDLDDMLSQRDLINGRLLSIVDEATNIWGVKVTR
                     IEIRDVRPPKELVAAMNAQMKAERNKRADILEAEGIRQAEILRAEGEKQSRILKAEGE
                     RQEAFLQAEARERAAEAEAKATQMVSEAIAKGDTTAINYFIAQKYTEALKEIGSSDNS
                     KVVLMPLEAGNLIGSVAGIAEILKSNKTRS"
     gene            complement(7998..8687)
                     /gene="ung"
                     /locus_tag="PWY43_001451"
     CDS             complement(7998..8687)
                     /gene="ung"
                     /locus_tag="PWY43_001451"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2136323.1"
                     /translation="MKTWTEALGEEKQKAYFQQILHYVHNERLAGKVIYPPQNEVFSA
                     FALTEFSDVKVVILGQDPYHGPNQAHGLAFSVKPPVAPPPSLVNMYKELSEDIEGFQI
                     PPHGYLIDWAKQGVFMLNTSLTVEQGRAGSHSQIGWEIFTDKVIAQLNQHRDNLVFLL
                     WGSHAQRKGQFIDRNRHCVLASPHPSPLSAYRGFFGCKHFSKANNYLISQGISPINWQ
                     LPVDVSYSSTH"
     gene            8846..9229
                     /gene="grcA"
                     /locus_tag="PWY43_001452"
     CDS             8846..9229
                     /gene="grcA"
                     /locus_tag="PWY43_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDL2136324.1"
                     /translation="MIKGVQITESSNSNLLNSFWLLDEAKNEARCLAAKAGFEEDQVV
                     AISELGDIAYREVPVNVAPTIKVEGGQHLNVNVLRRETLEDAIQNPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVITRTFTESL"
     gene            9403..9861
                     /locus_tag="PWY43_001453"
     CDS             9403..9861
                     /locus_tag="PWY43_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2136325.1"
                     /translation="MASIRQISDQTAAYQPIPLYRDFNSSRSVKLDLNSLCIKRPRDT
                     FFIHVKNPNLIAWGIELDDLLIVEQTNQYLVNDLLVLEKDGEYKFYQFFNEIQNGEGI
                     AEKILFSLDVSESNLRITDWAEVKIAGVITNVVHQMRTRGAFTQAKQRVA"
     gene            complement(9928..10500)
                     /locus_tag="PWY43_001454"
     CDS             complement(9928..10500)
                     /locus_tag="PWY43_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2136326.1"
                     /translation="MKKNLFATTFKPVFIASILSVFGGYSSIAVASLSSKPATPNQII
                     ASKAERLAKSSSVFTNRANLPTSSAHTANYSGRINSVYHNWVGTRYRLGGITKAGIDC
                     SAFVREVMGKAFSKDMPRSTAEQRHVGHSISKSDLRPGDLVFFRKNNHVGVYIGNGKF
                     VHASSSRGVTTSSLSESYWSRNYTQSRRVI"
     gene            complement(10628..11473)
                     /gene="nfo"
                     /locus_tag="PWY43_001455"
     CDS             complement(10628..11473)
                     /gene="nfo"
                     /locus_tag="PWY43_001455"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL2136327.1"
                     /translation="MKYIGAHVSASGGVENAVLRSVEIGANAFALFTKNQRQWQAPAL
                     KAETIEKFKRFCKVHHFSAEQILPHDSYLINLGNPEAEALEKSRVAFIDEMSRANQLG
                     LKLLNFHPGAHLKQISEQDCLARIAESINIAVEKVPNVIAVIENTAGQGSNLGWHFDH
                     LATIIEQVEDKSRVGVCLDTCHLFSAGYDISSYEKSEAVFNDFNQTVGFHYLRGMHLN
                     GSKTPLGSRVDRHHTLREGTIGTEVFAFIMQNMHFNHIPMILETISPEIWADEIKFLR
                     TLELK"
     gene            11532..12101
                     /locus_tag="PWY43_001456"
     CDS             11532..12101
                     /locus_tag="PWY43_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDL2136328.1"
                     /translation="MNETTINDFSLLCRLFGNLFYRHPTDPILAGTFNWLAQGGLRQV
                     WALNTDSQSENALTLLQKNANPTQLAASYQALFAENGAISPTISAYGLSVNNFVAFRQ
                     ERAMPELENADHIGLLFLTASWIEDNLDSALAQKILFEQFLLPCAGKFLGKIEAFDNG
                     FYKALAQLSRDALSAMADELEESELEVVE"
     gene            12131..12514
                     /locus_tag="PWY43_001457"
     CDS             12131..12514
                     /locus_tag="PWY43_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136329.1"
                     /translation="MKTLKLGCIFSLALMLSSCTLTPEQQAERKAKQIRAEQDLQVQL
                     AKQCDVETAELMYQQFNPPLSQTEQEEKAFKKRYAEKVNAPMFQACYKLAWQNYKAQV
                     ELEEIRWNYDRDYMYRGWRYCYYCW"
BASE COUNT         4107 a         2520 c         2403 g         3571 t
ORIGIN      
        1 ggtttagatt aacccctatc ggtcgccagc tcgcccaact gccggtcgat cctcgcttag
       61 gcagaatggt cattgaggca gcaaaaaacg gctcgctgca cgaagtgatg atgattgtgt
      121 cagctctctc aatccaagac cctcgtgagc gtccgcagga aaaacagcag tcggcagacg
      181 acaaacaccg ccgttttgct gataaagaaa gcgatttctt ggcctttgtg aatttgtggc
      241 actttatcca agcccaacaa aaagaactgt cgaaaaacca gttccgtaac ctctgccgta
      301 aggattattt gaactacaac cgtgtgcggg aatggcagga tatttatcac cagctccgcc
      361 ttgcagttag agaaatgggc ttaaaaatta acagtgaacc ggcaagttat cagccaattc
      421 ataccgcttt actcgccggc ttgttgtcgc atatcggctt gaaagataat gaaaaaatgc
      481 actatttagg agcaagaaat gcccactttt tcattttccc gaactcggca cttttcaaaa
      541 aacaaccgaa atgggtaatg gcggcagagc tggtggaaac cactaaactt tgggggcgaa
      601 tggtcgctcg cattgagcca gagtgggtgg aaccgttggc ggtacacctc actaaatcca
      661 gttacagcga accgcactgg gcgaagtcaa aaggggcggt aatggcatcg gaaaaagtgt
      721 cgctctatgg cattccgatt gtggcgagtc gccctgtgaa ttatggctcg attgatcctg
      781 ccgtcagccg tgaggttttt atccgctcgg cgttggtgga aggcgaatgg cacaacaatt
      841 acaaattttt caaagaaaat caccgcttga tccgtgaagt tgaagcatta gaacacaaat
      901 cccgccgccg tgatattttg gtggacgagc aggtgctgtt tgagttctac gatcagcgga
      961 ttggcaccga agttgtgtcg agcaagcatt ttgatacttg gtggaaacag gcaagcaaga
     1021 aagatcctga actgctgaac tttgaaaaat cgttcctgat caacgacaac gccaaccttg
     1081 ctagcgagct ggatttcccg aatttctggc atcaaggcga tttaaaattc aaactgagct
     1141 atcagtttga ggtcggtaaa gatcacgatg gcgtaacggt gcatattccg ctgtcgctgc
     1201 ttaaccaagt ggaacccgaa ggctttgatt ggcaaatttc ggggctacgc cacgagctga
     1261 tcgtctcgct gattaaatct ttaccgaaat cgctacggcg gaattttgtg ccggcaccaa
     1321 attatgcgga cgccttttta gggcgagccg agccgtttaa aaagtcgttg ctggacagtc
     1381 taagccacga actacgccga atgacgggcg tattggtgga gcctgagcag tgggatttaa
     1441 gccaactgcc gccacatttg cgaatgacgt tccgtgtgat tgacgataaa ggcaagaaac
     1501 tgcaagagag cgaaaacctt gatgagctga aatttgcctt gaaagatgag gtgcagaaca
     1561 ctctgtctaa cattgccgat gacggtatcg agcaaaatgg tgtgcatatt tggaattttg
     1621 ccgaattacc acagttctat gagcagaaaa aagcccattt cagcgtgaaa gcctaccctg
     1681 cgattgttga tgaacaaaca gctgtggggg tgaaactgtt tgaaaccgag tttgaacaag
     1741 caagagcaat gcaagcagga cttcgccgtt tgctgttgct gaatgtgcca tcgccaatta
     1801 aatatctaca cgaaaaactg ccgaacaaag ccaagctggg cttatatttt gccccgttcg
     1861 gcaaagtgtt ggagctgatt gatgattgca ttgcctgtgc ggtagataag ctggtggaag
     1921 agttcggcgg ctttgtctgg acagaggaaa aattcactca actgcacgaa tttgtacggg
     1981 caaatttaaa cgacaaaacc gtagaaattg ccaaacaggt tgaaaaatgc ctcacactgg
     2041 catacgagct aaacaagcgg ttaaaaggca agctcgattt tacgatggcg tttgcgttaa
     2101 gcgacatcaa aacccaaatc gaagggctga tttaccccga ttttgtcacc aaaaccggct
     2161 acgcccgcct tgcagattta tatcgttatt taaccgctat cgaacgccgt ttggataaac
     2221 tgctggtgga cagcaacacc gaccgagcca aaatgctacg ggtggaagcg gtgcaagagg
     2281 catataaaca gctccttgct aaattgccaa aatccaaagc agtgccggac gaagtgctgg
     2341 aaattcgcta tatgatcgaa gaacttaggg ttagcctgtt cgcccaacag ctcggcacca
     2401 aatacccgat ttcggacaag cggattttga atgtgattaa tgaaataaag taatatcccc
     2461 tatacaacaa gcggtcagat aagtaaaaaa ttttgcaact ctgaccgctt gttttctcga
     2521 atacctattc ctctcgcttc agaagcaaac ctgctacatt tttttgatta aaaaaatctc
     2581 aagataaaaa ttagaaaact aaatatatca gaaagtttga tctttatcat taaatagatg
     2641 ataattattt gtaataagaa gattaaccac gtaaaatagt cttatttttt ggaggtaaaa
     2701 tcatgtataa aataaagcat tcatttaaca aaacacttat tgcaattagc atctctagtt
     2761 ttttatctat tgcatacgct acagaatcta tagagaatcc acagcctatc attcagttat
     2821 ctgagagtat tagtacgaaa tacagcggaa aaggcgttaa actaggtata ttggacgagg
     2881 gatttatgct taagcaccct cgtcacagcc aaaatattca cccgattaca catcaattaa
     2941 caacacctga tggtgaggta agaacctatg accctagtta tatccaattt gaggtaaagc
     3001 cttttgaaca aaatggagaa accgcttttg caactttatt cgaaactcac ggagcaggcg
     3061 tagcagggat catttctgcg aaggctgata aaactattgg gaatggctat agcggtggtg
     3121 tcgcaaaagg tgtagaactt tatgtgactt ccaaatcata tagaagaact ttagaagaag
     3181 tccttagaga tgtccagaaa gaattggaaa ataaggataa tgaggaggaa gaggaagaaa
     3241 aaaaacctaa cttagctgag ttgggaaaag ctgacttatt cgtcagtaaa gagaaggaag
     3301 tagtttttga gcgttctgag tgggcaaccg ggctgaataa attactggat aaaaaagttt
     3361 ttgcaatcaa caacagttgg aatcctgatc caattagtga cgatattcac gaaatcgata
     3421 aattttatca atccatcaca caaaataagc aaaatccgtt acttcaagct attatgagag
     3481 caaaagataa tgatacatta ttggtgtttg ctaacggtaa cgagagcaaa aaacaaccag
     3541 gcattatgac attattgcca cgttattttt ctgagcttga aaaacattta attgccgttg
     3601 ctgcagtaga taataaacaa caaattaccg agtattccaa ccattgtgga gcaagtaaaa
     3661 actggtgtgt atccgcaccg ggtaatttac atctgttaaa tgcagcccct gatgctaaga
     3721 aaaatccgca atatggatta tcaaaagatg aaggaacatc atttgcaaca cctgttgtca
     3781 ccgggtcatt agcagtattg aaagaacgtt ttgattatct gacagctacc caaattcgtg
     3841 atactttatt gaccacctct actgatttag gcgaaaaagg ggttgatagc gtttatggtt
     3901 gggggctaat taacctgaaa aaagcaatta atggcccaac tcaattctta aatgatgaaa
     3961 ctgtcaccgt aactcgtgat gaccattggt cgaatccact cgccagccaa tttaaaatta
     4021 caaaaaaagg ggacaaatca ctgcatttag acggcgaaaa tcatttagat acggtagcag
     4081 ttgaagaagg tagattggcc ttaaacggta aaactaaggt gaaaacaatc agtaaccatg
     4141 caaatttagc tgtcaatgga accgaagttg agcaaaatta ttcttcgtct ggacaaagcc
     4201 aattagaagt gttgggcaaa tctgggctaa ttgccaatgc tcaagccaat atacatttag
     4261 ccggctcgtt aaaaattgat gataaattaa cagaaaaaac cgaagcaggt gatgtttcag
     4321 cgaccatagt acagttgaaa gataaagcaa cttatcaagg cggatttaca caattagtcg
     4381 aaaacgagaa tttagccaaa cgagggttaa tccaagatct ctatttcaaa gacagcgaaa
     4441 ttatcgcaaa agtgaataag ccactaactg atgagaaagc cgatacaaac gggcaagccg
     4501 gtttagcatt attaaatgcg ttaagaacta cacctattgc ctatcgccgt ggttggtata
     4561 acgattggct gcaatctgca ctagaacaac gtaaattaga taatctgcac tatgctgtat
     4621 ctaacaatat ttatgcagat agtctcgaat tactacgtag ccaaaatgcc aaaggcttaa
     4681 cccaaacaca gcaacattta tttacggcat accatacgcc acaacaaacg gcagtttggg
     4741 ctgaacattt gaatcaaaaa caatcagctt catcgaagca taccgatgtt aaacactacc
     4801 aatcactatt gggagtaagc cacaaactgg cagataaaac gggacttagt gcaacacttt
     4861 cacagcaaaa aaaccgctta gcaaaaccgt tttcacaagc taccttaaag caaaccgcat
     4921 taaatattgg cttacgttat catttagatg acgcttggtt tagtgaagct acattgcaat
     4981 ttgctcgtca gaaatatcaa caaagccgtc gatttgctag ccatcagcta ggtactgcag
     5041 aaaccagagg ttcaacgctc ggtggtgaaa tgcggatcgg ttatcaattt atgccaaacc
     5101 aatggataat tgaaccaagc cttggcgtac aatggattca aaccaaaatg aatggtttaa
     5161 atgaaagtgg cgaacttgcg actcaaacgg ctgcaatgcg ttatcgtgat gtgaatattg
     5221 ttccaagcgt gaaattacaa cgtactttcc aacttgagca aggctcaatt tccccttata
     5281 tcgggctaaa ttacttgcac agattaagtg gaaaaacgac caaaattacc agcgacattg
     5341 caggaaaaac actacatagt gaagcaacgg tgaaacgtaa tcgccaatta aatggtgaaa
     5401 tcggggtgaa actgcattat aaaaactggt ttactacgat gaatttagat tacagccgag
     5461 taaaatcagc aaatctattt ggctggaaag ccagcattgg tttaacgttc taaaatagcc
     5521 ttgtggcata agcaaatacg cttatgccac aaactcttta tcttttcttt ccctaataaa
     5581 taataatact tgtactacat aatatatgaa caactccttc cattttccaa cttttattga
     5641 aatatttccg aaagaaaaaa gacgatttaa attactacgg gaatacttgc gttattcttg
     5701 gcataaagcg tggtgttatg ctgaatgtaa agaattcact aactacctta actattctcc
     5761 attatggaaa aaactattta accaaaattt atatcgtgtt aatgctttac taggaactta
     5821 ttgcgataaa aaatttaata agcgacaacg cctaaatatc attatggaga attttgcgta
     5881 tgctgaaaaa ttatttggtt tagcttattg taataaatta gtagagcaaa aaaatctttg
     5941 cttattggaa ttgagtgatg agctaaaatt ataccttagt ataaataata ttgatccttt
     6001 agaaggtttt ttctccctaa acattcaaaa taacgaaggg atccatatct atgatgcatc
     6061 gtttacattc ttagcttcaa ataaattatt agtatcctca attcaaggtc ctaatacagc
     6121 atcagcacaa gatcttataa agtccgcaac caaacaatta cacggtgttc gcccaatgtt
     6181 tatgttgatc aatgtattta aatttattgc acaacatttt gatctagaac tactaggcat
     6241 tccccataaa aggcaagcta aatatcgctg gaatgatcat tcaaaattat tatttaacta
     6301 tgatgcgttt tggcaagaaa atggaggggt tattactcca gataaatatt ggcatttacc
     6361 aacttctctt gatatacgag agttaaatac tattccaagt aaaaagcgtt ctatgtataa
     6421 aaaacgttat gatctattca acaaaattga aaaagtaata aagtccttgg ataagggaca
     6481 ataatattgt aaataatttc aaattttata ccgcttgcta ttaacaataa aaccttgaat
     6541 aattgttcaa ggttttgttt agtgttctat gactaaataa gaaacaactt attgcttacg
     6601 gacaaataag gtaatcccct ccactttctg gacttcaatc ttatctgcta cttgtaaatt
     6661 attccctata acacgccaag tcgtatcacc aaattttcct cgaactatgc cattaggtaa
     6721 gatttcaacc acaaagcctt gagctcctag catagcatgc tcccgagagt taagggaact
     6781 gctctgatcc tctttcttgt cttttccatg ctgatatttc caccaaatca gactaaaaat
     6841 agtcgcaatt atcgcaaata acgagatttg taacgcttgc cctaatgtaa aaagtgcaac
     6901 gatgaaagca agaataagcg aggcaaatcc ccaccacata ataaagacac ccggtaatac
     6961 taattctaat gcaagtagta caaagcctaa tatgctccag ccaatccaat taaaaaacca
     7021 gtccatactc cctccatata ctcaattaat ccgtttttag gctatgatct tgttttattg
     7081 ctttttaaaa tttcagcaat accggcaaca gaaccaatca aatttccagc ttctaacggc
     7141 ataagtacaa ctttactgtt atctgaagaa cctatttctt tcaaggcttc ggtgtatttt
     7201 tgtgcaataa aatagttaat cgctgtagta tcgcctttag caattgcttc agaaaccatt
     7261 tgagtggctt tagcttccgc ttccgcagca cgttcacggg cttctgcttg taaaaatgct
     7321 tcttgacgtt cgccctctgc ttttaaaata cgagattgct tttcaccttc cgcgcgcaga
     7381 atttcggctt gtcgaatccc ttccgcttca agaatatcag cacgcttatt acgctcagct
     7441 ttcatttgag cattcattgc cgcaaccagc tctttcggtg gacgaacatc cctaatctca
     7501 atacgggtga cttttactcc ccaaatatta gtcgcctcat cgacaatgga aagtaatcta
     7561 ccgttaatta aatcacgctg agagagcata tcatctagat ccatagagcc gagcacggta
     7621 cgcatatttg tcatggttaa attaacaatt gcttgctcta aatgattcac ttcataggca
     7681 gctcggcgag catcaactgt ttgaacaaaa catacagcat caatcgcaac acttgcatta
     7741 tcacgtgaaa taacctcttg cgatggaata tctaaaactt gctccatcat attcattttt
     7801 cgcccaatac gatcaataaa aggtacgaca atgttcaacc ccgggcttag cgttttagta
     7861 taacgaccaa aacgttctac tgtccaatga aagccctgtg gcacaatttt aatagtcgaa
     7921 cttaatgcaa ctaaaacaag cacaacaaat gcaatactaa caatcggtaa atcaaaattc
     7981 atcgtttgct ccttaaacta atgtgttgaa gaataactaa catctactgg taattgccaa
     8041 ttaattgggg atattccttg agaaatcaag tagttattgg ctttagaaaa atgtttgcaa
     8101 ccaaaaaatc cacgatatgc ggaaagtggt gatgggtgcg gagaagctaa cacacaatgg
     8161 cgattacgat cgataaattg cccctttctc tgagcgtggc ttccccaaag taaaaagact
     8221 aaattatcac ggtgttgatt taattgtgcg atcactttat cggtaaaaat ttcccaacca
     8281 atttgtgaat gcgaaccggc tctaccttgc tcaaccgtta agctggtatt taacataaaa
     8341 acaccttgtt ttgcccagtc aattaaatag ccatgcggtg gaatttgaaa tccttcaata
     8401 tcttcagata attctttata catattgacc agagaaggtg gcggggcaac cggcggttta
     8461 actgaaaagg ctaatccgtg ggcttgattt gggccgtgat aaggatcttg ccctaaaatt
     8521 actactttca cgtcactaaa ttcggttaag gcaaaagcac taaacacctc gttttgcgga
     8581 ggatagatga ctttgccggc taatcgttcg ttatgtacat agtgtaaaat ttgttgaaaa
     8641 tacgcttttt gtttttcttc gcctaaagct tctgtccaag ttttcataaa accaccaagt
     8701 gtatattttt tgcccaacca actcaatcat gtctaaaaat ggttaaattt ttatcgcttt
     8761 ttatctaaaa aggggtacaa taacttcaaa taaatttgaa tttttaaaat ttttttataa
     8821 tttcaatctt agaggagata tcaaaatgat taaaggtgta caaatcacag aatcatcaaa
     8881 cagcaactta ttaaactctt tctggttatt agacgaagct aaaaatgaag ctcgttgttt
     8941 agctgcaaaa gcaggatttg aagaagatca agttgttgct atcagcgaat taggtgatat
     9001 cgcataccgt gaagtacccg ttaatgttgc accaacgatt aaagttgaag gtggtcagca
     9061 cttaaacgta aacgttttac gccgtgaaac attagaagat gcgattcaaa accctgagaa
     9121 atatcctcaa ttaacgatcc gtgtttcagg ttatgcagtt cgtttcaact cattaacccc
     9181 tgagcaacaa cgcgatgtta ttactcgtac attcactgaa agcctataat aacagctttt
     9241 aagcataaga agcaccctat gggtgctttt ttattgcttt ttaaaggata acaagcagtc
     9301 aaaacactgg ttaaatttac aaaaaattcc aaaataatgg ttgacactgg tattttagcc
     9361 agtaaaatac cctgtataaa aaaacagata gaggatttta ctatggcttc aattagacaa
     9421 atttcagatc aaacggcagc ttatcagccg attccgcttt atcgggattt caatagcagc
     9481 cgctctgtaa agctcgatct taactcactt tgtatcaaac gcccaagaga cacttttttt
     9541 atccacgtga aaaacccaaa tttaatcgcg tggggaattg aactagacga tttattgatt
     9601 gtagaacaga ccaaccaata tttagttaat gatctattgg tgcttgaaaa agatggggaa
     9661 tataaattct atcaattctt taatgaaatt cagaatgggg aaggtattgc tgaaaaaatt
     9721 ttattttcat tagatgtgag cgaatcaaat cttagaatta ctgattgggc tgaagtaaaa
     9781 atagcaggtg taattactaa tgtggttcat caaatgcgta ctcgtggagc atttacacaa
     9841 gctaagcaac gcgtagcata aactttttta attaaaaaag aacactcgca aaagtgagtg
     9901 ttcttttcat cttggaattg taatttatta aattacacgg cgagattgtg tgtagtttct
     9961 tgaccagtag ctttctgaca atgagctggt ggttacccct ctacttgaac tagcgtgtac
    10021 aaatttacca ttaccaatat aaacccctac atggttattc ttacggaaaa agactaaatc
    10081 acctgggcgt aaatcagatt ttgaaatgga atgcccgaca tggcgttgtt ctgcagttga
    10141 acgaggcata tctttactaa atgctttgcc cattacttca cgcacaaagg cggaacaatc
    10201 aattcctgct tttgttatgc cgcctaaacg atagcgtgtc cctacccaat tatgataaac
    10261 actattaata cgaccactat aattcgctgt atgagcggaa gaagttggaa gatttgctcg
    10321 attggtaaat actgaagaag acttagctaa tctttctgct ttacttgcaa tgatttgatt
    10381 tggtgtggcc ggtttagaac ttaaacttgc tacagcaatg gaagaatacc ctccaaaaac
    10441 agacaaaata gaagcaataa atacgggctt gaaggttgtt gcaaaaagat tttttttcat
    10501 aatttatgtc tcgtctcgcc agaacaatta cagcggtaaa ttccttaact aaatcttcta
    10561 aaagattaaa aaagataaca aaataaggtc tagactctgc tagacccact actcagacca
    10621 gtataaccta ttttagttcc aatgtgcgta gaaattttat ttcatctgcc caaatttcag
    10681 gcgaaattgt ctctaaaatc ataggaatat ggttaaaatg catattttgc ataataaatg
    10741 caaacacttc ggttcctatt gtgccttctc ttaaggtatg atgacgatcc actctgctgc
    10801 ctaatggtgt ttttgaacca tttaagtgca ttccccttaa ataatgaaaa ccaaccgttt
    10861 ggttaaaatc attaaatact gcctcgctct tttcataaga actaatatca tagccggcag
    10921 aaaataagtg acaagtatct aaacacaccc ccactcggct tttatcctca acttgctcaa
    10981 taatagttgc caaatgatcg aaatgccaac ctaaattaga accctgtcct gccgtatttt
    11041 caatcacggc aataacattc ggtacttttt caactgcaat attgatcgat tctgcaattc
    11101 tagccaaaca atcttgctcg gaaatttgct ttaaatgcgc ccccgggtga aaattcaaca
    11161 attttaaacc taattgattt gctcgagaca tctcatcaat aaatgctaca cgagatttct
    11221 ccaatgcctc cgcttccgga tttcctaaat taatcaaata gctatcgtgt ggtaagattt
    11281 gctctgctga aaaatgatgc actttacaaa aacgcttaaa tttttcgata gtttcggctt
    11341 ttaatgccgg tgcctgccat tgacgttgat tcttagtaaa taacgcaaat gcattagcac
    11401 caatttctac ggaacgcaaa actgcatttt ccaccccacc tgaagcacta acgtgtgcgc
    11461 ctatatattt cattcagtcc tctcttgtgt tctgctatac taacaattct tttttctatc
    11521 tatttcaaat tatgaacgaa actaccatta acgatttttc cctgctttgc cgtttgtttg
    11581 gcaacttatt ttatcgtcac cctaccgacc ctattttagc tggcacattt aactggcttg
    11641 cccaaggagg attacgccaa gtctgggcat taaatacgga tagccaaagt gaaaatgcgc
    11701 tgactctctt acaaaagaat gctaacccta cgcaattagc agcaagctat caagcgcttt
    11761 ttgctgaaaa tggggctatt tctccaacaa tttcagccta tggattatca gttaataatt
    11821 ttgtagcttt tagacaagag cgggcaatgc ctgaattaga aaatgccgat cacattgggt
    11881 tactatttct cacggcatct tggatcgaag ataatctcga ttctgcctta gctcaaaaaa
    11941 tattattcga acaattttta ctaccttgtg caggcaaatt cttaggaaaa atagaagctt
    12001 tcgacaatgg tttttataaa gccctcgcac aacttagccg tgatgccctt agtgctatgg
    12061 ctgatgaact tgaagaaagt gagttagaag tggtagaata aatcaccaat ttcaactaga
    12121 ggattcagca atgaaaacac tcaaattagg ctgcattttt tcactcgcac ttatgttaag
    12181 tagctgtact ttaactccag agcaacaagc tgaacgtaaa gcgaagcaaa ttcgggcaga
    12241 acaagatttg caagttcagc tcgccaaaca atgtgatgta gaaacggcgg agttaatgta
    12301 ccaacaattt aatccgccac ttagccaaac cgaacaagaa gagaaagcat ttaagaaacg
    12361 ctatgccgaa aaagtcaatg ccccaatgtt ccaagcctgt tataagcttg cttggcaaaa
    12421 ttacaaggca caagtagaac ttgaagaaat tcgttggaat tacgatagag actatatgta
    12481 tagaggctgg cgttactgct attattgctg gtaaatctca ttgtatgagc ggtcattttt
    12541 ttaacacctt gcaaaaaatt ggcgagaaat gaccgcttat taattgatat aggtttattt
    12601 t
//
LOCUS       DANKBV010000063        12574 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.063, whole genome shotgun sequence.
ACCESSION   DANKBV010000063 DANKBV010000000
VERSION     DANKBV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.063"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            126..2402
                     /gene="gshAB"
                     /locus_tag="PWY43_001458"
     CDS             126..2402
                     /gene="gshAB"
                     /locus_tag="PWY43_001458"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL2136330.1"
                     /translation="MKLQKLIRENHLALLFQQGNFGLEKESQRVDRNGNIVTTPHPAV
                     FGNRSYHPYIQTDFAESQLELITPPNAKLEDSFRWLSAIHEVVLRSLPEEEYIFPLSM
                     PAGLPPEEQIQEAQFDNPEDVQYRQHLSKNYGKYKQMVSGVHYNFQLSTEFVEKAFAL
                     QTEYSELKAFQNALYMKLANNFLRYQWILLYLLAATPTVESQYFYEKSPLARGQLVRS
                     LRSSPYGYVNSEKVVVNHDSLESYVESLEANVANGLLIAEKEFYSNVRLRGGKKAREL
                     LENGVKYAEFRLFDLNPFAPYGIELNDAKFIHYFLLGMLWLEETSGQKEVEIGKQKLY
                     QVALEDPRTETAFRAEGEAILNQLLAMLEELSAGQESLDLVREKLAQFADPSKTLNGR
                     LIAAIEEVGSYKALGAKLAQTYKAQAFERFYALSAFDNMELSTQALFFDLIQQGIEVE
                     VLDENDQFLALKFGDHLEYVKNGNMTSRDQYISPLIMENKVVTKKVLAKAGFNVPKSV
                     EFTNLEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQAVKNREDFAKAVEIAFREDK
                     EVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVVGDGIHSVKELVERKNDDPLRGDGS
                     RSPLKKIALGEIEQLQLKEQGLTVDSVPAKGQTVQLRANSNISTGGDSIDMTDQMHES
                     YRQIAVGIAQAMGAKVCGVDLIIPDLTKPAEPNLSSWGVIEANFNPMMMMHIFPYQGK
                     SRRLTKAVIKMLFPEIGN"
     gene            2724..3701
                     /gene="manX"
                     /locus_tag="PWY43_001459"
     CDS             2724..3701
                     /gene="manX"
                     /locus_tag="PWY43_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL2136331.1"
                     /translation="MSIAIIIATHGVAAEQLLKTTEMLIGEQEDVAYIDFVPGENAET
                     IMGKYQALVEGPLSHCDQVLFLVDMWGGSPFNAANRFQDGKSGMDVVAGVNIPMLVNT
                     CMARDDVESLEELVEVALEAGSTGIRSLNHQPEVAEVQTATPAQETAAVQQPEIVDDG
                     RRMTIALARIDDRLIHGQVATVWTKESKVNRIIVVNEGVAKDSVRTTMLKSVAPLGVT
                     AHVVDVPKMIRVYNNPEYADDRVMLLFTNPSDVLALIEGGVPLNSINVGGISHKEGKQ
                     MVSHAVAVDQKDIDAFKAIDAKGVELEVRKVSSDSRQYMMDLLKKHNLI"
     gene            3713..4513
                     /locus_tag="PWY43_001460"
     CDS             3713..4513
                     /locus_tag="PWY43_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL2136332.1"
                     /translation="MELSALQIVFLFLVACISGMGSILDEWQTHRPLIACTMIGLVLG
                     DVTTGVIVGGSLELLALGWMNIGAAVAPDAALASIVSTILVIVGRQDISTGIAIAIPL
                     AAAGQVLTYVVRAITVGFQHAADKSIEDGNLSRLDWLHRGALLLQAMRIAIPALIVAL
                     TAGTDAVQTMLNAIPEVVTTGLKIAGGFIAVVGYAMVINMMRAGHLMPFFFAGFVIAG
                     FTDFNLVALGVLGTVIAMVYIHMHPKYNKSQQVVVAAAPKNDLDNRLD"
     gene            4527..5363
                     /locus_tag="PWY43_001461"
     CDS             4527..5363
                     /locus_tag="PWY43_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL2136333.1"
                     /translation="MTTEIKKVTKSDLNAVVRRSNLFQGSWNFERMQALGFAYAMVPV
                     IKRLYPDPNDPGRKEAIKRHSEFFNTQPFVAAPILGVTIAMEEERANGKEIDNAAING
                     IKVGLMGPLAGVGDPIFWGTIRPVFAGLGAVLALKGELLGPILFFALFNIVRLAARYY
                     GVVYGYKKGLDVVQDMSGGLLQKLTEGASILGLFIMGALVQKWTSINVPLVLSTIQKQ
                     DGTTEVTTVQSILDSLMPGLLPLLLTFACMWLLRNRVNAIWIILGFFVIGILGASTGA
                     LA"
     gene            5456..5941
                     /locus_tag="PWY43_001462"
     CDS             5456..5941
                     /locus_tag="PWY43_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDL2136334.1"
                     /translation="MTNTILILFILLGLGFAIYDQVFMHKLKGTTLLEIRLKKQKTID
                     TWLLIGLIVLSISYGVQNQIQPFTLYLLATCIILSVYLAFFRTPRLLLKQHGFFFANV
                     FFNYDKIYQVNIAEGKADEKILVIDLHSGRRLLADVENAEDLQPVVDFFGGYKKESEQ
                     K"
     gene            complement(6028..6819)
                     /gene="tpiA"
                     /locus_tag="PWY43_001463"
     CDS             complement(6028..6819)
                     /gene="tpiA"
                     /locus_tag="PWY43_001463"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL2136335.1"
                     /translation="MARRPLVMGNWKLNGDKAFTKTLIADLKAELHGVEGCDVAIAPP
                     VMYLAHAEASLAGCGCGCSDKGLIALGSQNVDVNVKGAFTGDISPEMLKEFGAKYIII
                     GHSERRTYHKESDEFIAQKFKVLKDAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
                     NAHGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAAKSQAVADQVIIQY
                     GGSVNDANAAELFTQPDIDGALVGGASLKAPAFAVIVKAAAAAKA"
     gene            6979..7998
                     /gene="truD"
                     /locus_tag="PWY43_001464"
     CDS             6979..7998
                     /gene="truD"
                     /locus_tag="PWY43_001464"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDL2136336.1"
                     /translation="MKLNYLYSPPQAVGRLKAEYADFIVREELGYELAGEGEFVAVKI
                     RKTSANTLFVGEKLAKFVGISEREMSYAGLKDRHAVTEQWFCLHLAGKETPDFSTFEC
                     EGVEVLEVTRHNRKIRVGSLAGNHFELLLRDVTESEELIYRLQQLQAVGFPNYFTEQR
                     FGRDGHNLTQALRWANGEISVKDRKKRSFYLSAARSEVFNLVVSQRISDGLIQKVLKN
                     DYVQLAGSNSFFCVKSDEIAETQARLTQQDLLLTAPLIGERSLELECNPAEKTIIEQH
                     AQLVALMQKERMAAARRAMLCKPQNLEWQFEQEGLRLKFFLDSGSYATALVRELILLA
                     EQNEA"
     gene            8161..8949
                     /gene="surE"
                     /locus_tag="PWY43_001465"
     CDS             8161..8949
                     /gene="surE"
                     /locus_tag="PWY43_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDL2136337.1"
                     /translation="MRILISNDDGFHAVGIQTLAKTLREAGHSVTIIAPDRNRSAASS
                     CLTLVDPLRVHRFDEYNYAVIAGTPADCVHLALNGLFDGKFAEKFDLVVSGINHGANL
                     GDDVVYSGTVAAALEGRHLPLPSIAVSLVGRKHSSFLDGENHFGTAAQVVLDLLEKME
                     NQPFPQNHVLNVNVPNLPYSELKGTMITRLGARSTAAEIVKQKDPRNADVYWIGVTGD
                     PIDESFGTDFYALNRDCVSITPIQVDMTAHKTIATLKGIFNETV"
     gene            8936..9520
                     /locus_tag="PWY43_001466"
     CDS             8936..9520
                     /locus_tag="PWY43_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL2136338.1"
                     /translation="MKLFETIYDKTMQWSKHRFAAFWLSFVSFIEAIFFPIPPDVMLI
                     PMSMAKPQNAFKYAVYTTLASVAGGVIGYFIGLYATDWVQGVIANWGMQANFDKAKLW
                     FETWGVAVVFLAGFSPIPYKVFTICAGVMQMAFFPFVITAAISRFARFILVAKLSAWG
                     GKKYEQKIRRSIELIGWGTIVLAVVAYIIYTLMK"
     gene            9538..10038
                     /locus_tag="PWY43_001467"
     CDS             9538..10038
                     /locus_tag="PWY43_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2136339.1"
                     /translation="MKKSLFVLSLISFALVGCSSNSGESTSAVETTEANATWQSADSI
                     QTAPMPASMNQPIATQPTYQQPTYSAPQPISAAPAPMPVSSGSDSSQVETVGNCQVVR
                     DANNAPIYSQITKGCYTESHYTVGKSDTLYLIGYLTGTNANQIAQLNGLNTGSPLKVG
                     QVLRVR"
     gene            10051..10251
                     /locus_tag="PWY43_001468"
     CDS             10051..10251
                     /locus_tag="PWY43_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136340.1"
                     /translation="MNRTLLAFAISCAFLTACSSPTQPQVEDPNMLPAGTLQPIEGSG
                     ASQDSYSWPSDIQSTSMPDSMK"
     gene            10270..11550
                     /locus_tag="PWY43_001469"
     CDS             10270..11550
                     /locus_tag="PWY43_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2136341.1"
                     /translation="MKKQFILLPLVSAVLAACSSNNPAPVVNASGNNELSPGVMQPVD
                     VSAGAINSSGGWQSDIQSAPMPSSMNTTPVQTMPQPVSVPQPVATVPQPITNPVITEQ
                     PPQPTTTTKIVKKTKTVEKKVDQNFEIPRDANNAPMYNQIQKGFYDGSTYTVRKGDTM
                     FLIAYIVGKDVKEIAALNNMSEPYQLSVGQKLKTGKSATKTITVEEKVTVPVEPQITY
                     QQGANGTTYASGGNITGPVKAGTANADVTPVNNGIKATTATAPAVTASVGTVNATEDR
                     TASTIRATDGTASGAVQSSNTVSAPASSIKWQWPTSGRVISGFSSAEGGNKGIDIAGN
                     KGQDVRAAAAGKVVYAGNALQGYGNLIIIKHTDDFLSAYAHNNTIEVDEQETVKAGQK
                     IATLGSTGTNTNKLHFEIRYKGKSVDPARYLPRK"
     gene            11974..12540
                     /locus_tag="PWY43_001470"
     CDS             11974..12540
                     /locus_tag="PWY43_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL2136342.1"
                     /translation="MSETKIRCDWCKSSEIYMKYHDEEWGKPEFDSLKLFEKICLEGQ
                     QAGLSWITVLKKREAYRQAFHQFNPEKIAKMGEEEIDLLMQNTSLIHHRAKLEAIIKN
                     AKAYITMQQNGEDFSRFIWSFVNYQPQINDVPTLEAVSAHTETSKAMPKALKKRGVVF
                     VGETTCYAFMQSMGLVNDHVNNCVFKHK"
BASE COUNT         3718 a         2588 c         2718 g         3550 t
ORIGIN      
        1 gaagcagtat ttctgttata accaaatcat ctacttcatt ccaataaact aaaacccact
       61 atttcactaa gtctttcatt tgtgttattg tgccgataca tttttttgac tacaaaggat
      121 agatgatgaa actacaaaaa cttatccgag aaaatcattt agcattgcta tttcaacaag
      181 ggaattttgg gcttgagaaa gagagccaac gggttgatcg caacggcaat atcgttacta
      241 ctccccaccc tgccgtattc ggtaatcgct cttaccaccc ttatattcaa accgattttg
      301 ccgaaagtca gctagagtta atcactcccc cgaatgctaa attagaagac agttttcgtt
      361 ggctctctgc tattcacgaa gtggtattgc gttcgttgcc agaggaggaa tatatcttcc
      421 ctctcagtat gccggcaggc ttgccgccgg aagagcaaat tcaagaagca caatttgaca
      481 accctgaaga tgtgcaatat cgccaacacc tttccaaaaa ctatgggaaa tataagcaaa
      541 tggtaagcgg cgtgcattat aattttcaac tttcaacgga atttgtcgaa aaagcctttg
      601 ctttacaaac ggaatatagc gagctgaaag ccttccaaaa cgcactttat atgaagttag
      661 ctaataactt tttgcgttat caatggattt tgctctattt attggctgcc acgcctacgg
      721 tggaatcgca atatttttat gaaaaatcac cgcttgcgag agggcaactt gtccgcagct
      781 tgcgttcaag cccttatggt tatgtgaatt cggaaaaagt ggtggttaat cacgacagct
      841 tagaaagcta tgttgaaagc cttgaagcca atgttgccaa cggattatta attgccgaaa
      901 aagaattcta ttccaatgtg cgtttacgag ggggcaaaaa agcccgtgag ctattggaaa
      961 acggtgtgaa atacgccgaa ttccgtttat ttgatttaaa ccctttcgcc ccttacggca
     1021 ttgagctgaa tgatgccaaa tttatccatt atttcttact cggtatgctt tggctggagg
     1081 aaacaagcgg tcaaaaagag gtcgaaatcg gcaaacagaa actttaccaa gtcgcacttg
     1141 aagatccaag aacagaaacc gctttccgtg ccgaaggtga agccatttta aatcaacttt
     1201 tagcgatgct tgaagaatta tctgccggtc aggaaagcct agatttggtg cgggaaaaat
     1261 tagctcaatt tgctgatcca agcaaaaccc tgaacggacg tttaattgcc gcaatagagg
     1321 aagtcggcag ttataaagca ttgggggcta aattggctca aacctataag gcacaagcct
     1381 ttgagcgttt ttatgcgtta tcggcatttg ataatatgga actttccacc caagccctgt
     1441 tttttgattt gatccaacaa gggattgagg tggaagtact ggacgaaaac gatcaattcc
     1501 tcgccctgaa atttggcgat catttggaat atgtgaaaaa cggcaatatg accagccgag
     1561 atcaatacat ttcaccgctg attatggaaa acaaagtggt gacgaaaaaa gtattggcaa
     1621 aagccggctt taacgtgccg aaaagcgttg aatttaccaa ccttgaacag gctgtggcac
     1681 attatccgct atttgaaggc aaagcggtgg tgattaagcc gaaatcgact aactatggtt
     1741 tagggattac catcttccaa caagcggtca aaaatcgaga agattttgca aaagcggttg
     1801 aaattgcgtt ccgtgaagac aaagaggtga tggtggaaga ctatttagtc ggtactgaat
     1861 accgcttctt tgtactgggc gatgaaaccc ttgcggtgtt actgcgtgta ccggcgaatg
     1921 tagtgggaga tggcattcac agtgtaaaag agctggtgga gcgtaaaaat gacgatccgc
     1981 tacgtggcga tggctctcgt agcccgttga aaaaaattgc cttaggtgaa attgagcagt
     2041 tacaacttaa agagcaaggc ttaaccgtag attccgtacc ggcgaaaggt caaacagttc
     2101 aactgcgtgc caattccaac atcagcaccg gcggcgattc gattgatatg accgaccaaa
     2161 tgcacgaaag ctatagacaa attgcagtgg gaattgctca ggcaatgggg gctaaagtgt
     2221 gtggggtgga tttgatcatt cctgatctca ccaaacctgc cgagccaaat ttaagctctt
     2281 ggggcgtcat tgaggcaaac ttcaacccaa tgatgatgat gcacattttc ccttaccaag
     2341 gcaaatcccg ccgtttaacc aaagcggtaa tcaaaatgct gtttcctgag ataggcaatt
     2401 aaattttctc tctatacctc ttttaaggag aatttactca agaattctcc ttaatttatt
     2461 tctaaagttt tactccaatt aaatttcact ttgtgcaaaa cactccaaaa gaagaatttt
     2521 tttgtataag taaaaacgat cgaaaatatc tccgttaaca aaatgttata aagaaaagac
     2581 atttagccca atttatagcc aaaaaagctt attgtctgtg atatagatca cacttttgta
     2641 attaatacat tgtgtgaaat aattatcata ttaaaatctc gccccagtaa ataaacaact
     2701 taaaaaaccg caaggagtaa caaatgagca ttgctattat catagcaacc catggtgttg
     2761 ctgcagagca acttctcaaa acaactgaga tgttgattgg tgaacaagaa gatgttgctt
     2821 acattgactt tgttcccggc gaaaatgcag aaacgattat gggcaaatat caagcattgg
     2881 ttgaaggtcc actatcacac tgtgaccaag tgctgttttt ggtggatatg tggggaggaa
     2941 gcccattcaa cgccgcaaac cgattccaag atggaaaatc gggtatggat gttgttgcgg
     3001 gtgtgaatat tcctatgtta gtgaacacct gtatggctcg tgatgatgtg gagtcgttgg
     3061 aagaattggt ggaagtcgca ttagaagccg gctcaaccgg gattcgttca cttaatcacc
     3121 aacctgaagt ggctgaggtg caaaccgcta ctcctgcaca agaaactgca gctgttcaac
     3181 agcctgaaat tgttgatgac ggacgtcgta tgaccattgc ccttgcccgt attgatgacc
     3241 gcttgattca cggtcaagtt gcgacggttt ggacaaaaga aagtaaagtc aatcgcatta
     3301 ttgtggtaaa cgaaggtgta gcaaaagata gtgtacgaac tactatgcta aaaagtgttg
     3361 ctcctcttgg cgtaacggct cacgttgttg atgtacctaa aatgattcgt gtctataaca
     3421 accctgaata tgccgatgat cgtgtaatgt tactctttac caacccaagt gatgtcttag
     3481 ccttaattga aggcggtgta ccactcaata gcattaacgt gggcggtatt tcgcataaag
     3541 aagggaaaca aatggttagc catgctgtgg cggttgatca aaaagatatt gatgccttca
     3601 aagcaattga tgcgaaaggt gttgaattag aagtacgcaa agtctcaagc gatagccgtc
     3661 agtatatgat ggatttattg aaaaaacata acttaatcta aaggaaatta ctatggaact
     3721 ttcagcatta caaattgtgt ttttattcct cgttgcctgt atctcaggta tgggctcaat
     3781 tcttgatgaa tggcaaaccc accgtccgtt aattgcctgt acgatgatag gcttagtgct
     3841 tggtgatgtg actaccggcg ttattgttgg cggctcatta gaattattag ccttaggttg
     3901 gatgaacatt ggtgccgctg ttgcaccaga tgctgcatta gcctctattg tctcaaccat
     3961 tttagtcatt gtaggtcgtc aagatatttc aaccggtatc gccatcgcta ttccgcttgc
     4021 agctgccggc caagtattaa cctatgttgt gcgtgcaatc accgtgggtt tccaacacgc
     4081 agcagataaa tcaattgaag acggtaactt aagccgttta gactggttac atcgcggtgc
     4141 tttattgctt caagcaatgc gtattgccat tccggcatta atcgtggcat taacagcagg
     4201 tacagatgcg gttcaaacaa tgttaaatgc cattcctgaa gtggttacaa ccgggctgaa
     4261 aattgccggt ggcttcattg ccgtggtagg ttatgcgatg gtaatcaata tgatgcgtgc
     4321 aggtcattta atgccgttct tcttcgctgg ctttgtaatt gcaggcttta ccgattttaa
     4381 ccttgttgcc cttggagtat taggtacggt tattgcaatg gtttatatcc atatgcaccc
     4441 taaatataac aaaagccagc aggtagtggt ggcagctgca ccaaaaaatg atttagacaa
     4501 ccgtttagac taagagggca tttaaaatga caactgaaat caaaaaagta acaaaaagcg
     4561 atttgaatgc cgttgtacgt cgttcaaact tattccaagg ttcgtggaac tttgagcgta
     4621 tgcaggctct cggttttgcg tatgcgatgg taccggttat caaacgttta tatccggatc
     4681 caaatgaccc gggtcgcaaa gaagcgatta aacgccattc tgaattcttt aacactcaac
     4741 cattcgtagc tgcaccgatt ttaggggtaa cgattgcgat ggaagaggaa cgagccaacg
     4801 gtaaagaaat cgacaatgcc gcaattaacg gtattaaagt tggtttaatg gggccgttag
     4861 ccggtgtggg tgatccaatt ttctggggaa ccattcgccc tgtatttgcc gggttagggg
     4921 cagtcttagc cttaaaaggc gagcttttag gtccaatcct gtttttcgcc ttatttaaca
     4981 tagtgcgttt agcagcacgc tattatggcg tagtgtatgg ctataaaaaa gggttagatg
     5041 ttgtgcaaga tatgagcggc ggtttattgc aaaaattaac cgaaggtgct tcaattttag
     5101 gtctgtttat tatgggggca ttggtacaaa aatggacgag cattaacgtt ccgcttgtgc
     5161 tttcaaccat tcaaaaacaa gacggtacaa ctgaagtaac aacggttcaa agcattctcg
     5221 atagcttaat gccaggctta ctcccattat tattaacctt tgcttgtatg tggttactgc
     5281 gtaatcgagt aaatgctatc tggattatcc tcggtttctt tgttatcggt atcctcggcg
     5341 cttcaaccgg tgcattagct tagttgcaaa attttctgtg aaaaagaccg cttgaacttg
     5401 aacattcaag cggtttttta tacgctttag cctaaataaa ttcacttgct taattatgac
     5461 caataccatc cttattctct ttattttact tggcttaggg ttcgccattt atgaccaagt
     5521 ttttatgcac aaactcaaag gaacaacctt actggaaatc cgtttaaaaa agcaaaaaac
     5581 aattgatact tggttattga ttggtttaat cgtcttgtca attagctatg gcgtacaaaa
     5641 tcaaatccag ccctttacac tctacctgct ggcgacgtgc attattctgt cggtctatct
     5701 cgcctttttc cgcacccctc gcttgttgtt aaaacagcac ggttttttct ttgcgaatgt
     5761 cttttttaat tacgacaaga tttatcaagt caatattgca gaaggaaaag cagacgaaaa
     5821 aattttggta attgatttac atagcggaag aaggctactt gccgatgttg aaaatgcgga
     5881 agatcttcaa ccggtagtgg atttttttgg cggttataaa aaggaaagtg agcagaaata
     5941 gtttgcaaac aaaacaacaa gcggtcattt ttccctgaaa ttttgcaaaa aaccgaggaa
     6001 agatgaccgc ttgtaaaatc ggttagatta ggctttagca gccgctgccg ctttaacaat
     6061 caccgcaaat gcaggcgctt taagtgaagc accacctact aatgcaccat caatatccgg
     6121 ttgagtgaat aactctgctg cattggcatc gtttacagag ccaccgtatt gaatgatcac
     6181 ttggtcagcc actgcttgtg atttagctgc aatatggtca cgaataaatt tatgtactgc
     6241 ttgtgcttgt gccggagtgg ctgatttgcc ggtaccgatt gcccaaattg gttcgtaagc
     6301 aataactgca ccgttgaatg cttccacacc gtgtgcgttg atgaccgcat caatttgtct
     6361 tgcacacact tcttcggttt tacctgcttc attttcggct tcagattcac cgatacataa
     6421 tacaggcact aagccggcat cttttaatac tttgaatttt tgagcaataa attcatcgct
     6481 ttctttgtgg taagtacggc gttctgaatg cccgataata atatatttcg ccccaaactc
     6541 ttttaacatt tcaggtgaaa tatcaccggt gaatgcccct tttacgttca catccacgtt
     6601 ttgtgaacca agtgcgatta agcctttgtc gctacaacca cagccacaac cggcaagtga
     6661 tgcttcagcg tgtgccaaat acatcaccgg tggagcgatt gctacatcac agccttcaac
     6721 gccgtgtaat tctgctttta aatctgcaat cagggttttg gtaaaggctt tatcaccgtt
     6781 taatttccag ttacccatta caagtggacg acgtgccatt ttgtttctcc tatatgaagt
     6841 tgaacttaaa tctaacatca agcactatac caaattttgt agccgttttg tgctttttat
     6901 tttgcttttt atgatctaga tcgaaaggtt taacgtataa tttctcaatc ttacgtatat
     6961 ccttaatatg agaacctgat gaaactgaat tatttatact ctcctcctca agctgttggg
     7021 cgactaaaag ccgaatatgc agactttatt gtgcgtgaag aactgggcta cgaactagcc
     7081 ggcgaagggg aatttgtggc ggttaaaatc cgcaaaacca gcgccaacac gctgtttgtg
     7141 ggggaaaagt tagccaaatt tgtcggcatt tccgagcgtg aaatgagtta tgcgggctta
     7201 aaagaccgcc acgctgtgac tgagcagtgg ttctgcttgc atttagcagg caaagaaacg
     7261 cccgatttct ccacctttga atgcgaaggc gtggaagtgc tggaagtgac ccgccacaac
     7321 cgcaaaattc gggttggctc tttagccggc aatcattttg aattattact gcgtgatgtc
     7381 acagaaagcg aagagttgat ttaccgtttg caacagttac aagcggtcgg atttcccaat
     7441 tattttaccg aacaacgctt tgggagagac ggacataacc tgactcaagc cctgcgttgg
     7501 gcaaatgggg aaattagcgt gaaagatcgc aaaaaacgta gtttctacct ttctgctgcc
     7561 cgctcggaag tgtttaattt agtggtttca cagcggatta gtgacggctt aatccaaaaa
     7621 gtattaaaaa atgattacgt tcaacttgcc ggctctaaca gctttttctg cgtgaaatcg
     7681 gacgaaattg ccgagactca agcccgttta acacagcaag atctacttct caccgccccg
     7741 ttgataggcg aaagatcgct tgaattagaa tgcaatcctg ctgaaaaaac tatcatcgaa
     7801 caacacgctc aactggttgc attaatgcaa aaagagcgta tggcagccgc tcgccgagct
     7861 atgctttgta agccacaaaa tcttgaatgg caatttgaac aagagggttt acgtttaaaa
     7921 ttctttttag attcaggcag ttatgccact gcactggtgc gagagttaat tctacttgcc
     7981 gaacagaacg aagcctaagc actcacaagc ggtaagattt aaatgatttt ttgcaatttg
     8041 taaaactttg gtaaaatctt accgcttttt acactttaga ctaaaaaaac tgtcgaaaaa
     8101 ttgttagaat tgcgatcttt gtatttagac tctatcaaga tgtaagatct aaaggaaatt
     8161 atgcgaattt taattagtaa tgacgatggt tttcacgctg tcggcattca aaccttagcc
     8221 aaaaccttgc gtgaagccgg acatagcgtg accattatcg caccggatcg taaccgcagt
     8281 gcagcttcaa gttgcttaac cttagttgat ccgctacgag tgcatcgttt tgatgaatat
     8341 aattatgcag tgatcgccgg cacgcctgcc gattgcgtac atttagcctt aaacggctta
     8401 tttgacggca aatttgcgga aaaatttgat ttagtggtct ccggcattaa tcacggagcc
     8461 aatttaggcg atgatgtagt ttactccggc acggtagcgg cagcgttaga aggtcgccac
     8521 ttaccgttac cgagtattgc agtatcactg gttgggcgta aacattcttc gtttttagac
     8581 ggtgaaaatc attttggaac ggcagcgcag gtagtgttag atttattgga aaaaatggaa
     8641 aaccagccat tcccacaaaa ccacgtccta aacgtgaatg tgccgaattt accttattca
     8701 gagctaaaag gcacaatgat cacccgcttg ggtgctcgtt caactgcagc ggaaattgtg
     8761 aagcaaaaag atcctcgcaa tgccgatgtt tactggattg gcgtcaccgg tgatccgatt
     8821 gatgaaagtt ttggcaccga tttttatgca ttaaaccgag actgtgtctc tattaccccg
     8881 attcaggtgg atatgaccgc tcataaaacg atagcaacct taaaaggcat ttttaatgaa
     8941 actgtttgaa acaatttatg acaaaacaat gcagtggtct aagcaccgtt tcgctgcttt
     9001 ttggttgagt tttgtcagtt ttattgaagc gatctttttc ccgattccgc cggatgtgat
     9061 gttgattccg atgtcgatgg caaaaccgca aaatgcgttt aaatatgcag tttacactac
     9121 ccttgcttcg gttgccggtg gtgttatcgg ctattttatc ggcctatatg ctaccgattg
     9181 ggtgcaaggc gtcatcgcaa attggggaat gcaagcaaac tttgataaag cgaaattatg
     9241 gtttgaaact tggggggttg cggtagtatt tttagccggt ttttcgccga ttccctataa
     9301 ggtatttacc atctgtgccg gtgtaatgca gatggcgttc ttcccgtttg tgattactgc
     9361 tgcgatttct cgctttgccc gttttatttt ggtagcaaaa ctctctgctt ggggtggcaa
     9421 aaaatatgaa caaaaaattc gccgctcaat tgaattaatt ggttggggaa caatcgtttt
     9481 agccgttgtt gcctatataa tttatacatt aatgaaataa attttaagga tataaaaatg
     9541 aaaaaatcac tttttgtttt atctctgatt tcttttgctt tagtgggttg ttcaagcaat
     9601 tcgggtgaat ccacttctgc ggtagaaaca acagaagcaa atgcaacttg gcaatcagca
     9661 gatagcattc aaactgcacc aatgcctgcc tcgatgaatc aacctattgc cactcagcca
     9721 acctatcagc agccgactta ctctgcacca caaccaattt cggcagcacc agcaccaatg
     9781 cctgtttcaa gtggtagtga tagctctcaa gttgaaacag tagggaactg ccaagtggtg
     9841 cgtgatgcga ataatgctcc tatctattca caaattacca aaggctgcta cacagaaagt
     9901 cactatacgg taggtaaaag tgatacgctt tatttaatcg gttatctaac cggcaccaat
     9961 gccaatcaaa ttgcgcagct aaatggttta aataccggct ctccactgaa agtcgggcaa
    10021 gtcttacgtg ttcgttaata ggcagagaaa atgaacagaa cccttttagc atttgcaatt
    10081 tcttgtgcat ttttgaccgc ttgtagctcg cctactcagc cacaagttga agatccaaat
    10141 atgttacctg caggcacatt acaaccgatt gaaggctcag gcgcaagcca agacagttac
    10201 agttggccgt ctgatattca atcaacgtca atgcctgaca gtatgaaata attttaggac
    10261 aatcatttaa tgaaaaaaca atttattctt ctccctttag tctctgctgt tttggcagcc
    10321 tgttcttcaa acaatccagc accggtggtg aatgcttccg gtaataacga attaagcccg
    10381 ggcgtgatgc agcctgttga tgtgagtgca ggtgcaatta attcaagcgg tggctggcag
    10441 tctgatattc aatctgcacc aatgccaagc tcgatgaata ccactccggt gcaaacaatg
    10501 ccccagccgg tttcagtgcc acaacctgtg gcgactgtac cgcagccaat taccaatccg
    10561 gtgattaccg aacaaccacc acagccgaca accacgacca aaatcgtgaa aaaaacgaaa
    10621 acggtcgaga aaaaagtgga tcaaaatttt gagatcccgc gtgatgccaa caatgcccca
    10681 atgtacaacc aaattcaaaa agggttctac gatggctcta cctataccgt acgcaaaggc
    10741 gacacaatgt ttttgattgc ctatattgtg ggtaaagatg taaaagaaat cgccgctctt
    10801 aataatatga gcgaacctta ccaattaagc gtagggcaaa aactcaaaac cggcaaatct
    10861 gcaacgaaaa ctattaccgt ggaagaaaaa gtcaccgtac cggttgaacc acaaattacc
    10921 taccaacaag gtgcaaacgg cacaacctat gcttctggcg gtaatattac tgggccggta
    10981 aaagcaggca cagccaacgc tgatgtcacg ccggttaata acggtattaa agcaacaact
    11041 gcaactgctc cggcagtaac tgcttctgtc ggtacagtaa atgcaacaga ggaccgtacc
    11101 gcctccacca ttcgtgccac agatggcaca gcaagtggtg ccgtacaaag tagtaatact
    11161 gtttctgcac cagcttcatc aattaaatgg caatggccaa ccagcgggcg tgtaatttca
    11221 ggcttctcct cagcagaagg aggtaataaa ggcattgata ttgccggcaa taaaggacaa
    11281 gatgtacgtg ctgccgccgc aggtaaagtg gtatatgccg gtaatgcctt gcagggttac
    11341 ggtaacttaa tcatcatcaa acataccgat gatttcttaa gtgcttatgc acacaacaat
    11401 actattgaag ttgatgagca agaaacggtg aaagccggtc agaaaatcgc gactttaggt
    11461 agtacaggaa ccaatacgaa taaattacat tttgaaattc gttacaaagg aaaatctgtc
    11521 gatccggctc gttatttacc aagaaaataa gagtaacttt ttagcataat ccgaccgctt
    11581 gtgtactaca agcggtcaat ttttaggaaa attatgcaaa tgaaaaaata tatcgcacac
    11641 atgataagtt cgttactgct gatagcttgt tcggactacc gagcaacacc aactagccca
    11701 agcgatgctt acacttgctc tccttctttt cgctactctt tcttaaaaaa acgtgcatac
    11761 ctttattcag tatagccgac attcaattat tggatccaac cgagccttca catcgcattt
    11821 atattttgtt atctgaagat cgatttcaag ccgaagtctt ctcaacgaag ctctctgaat
    11881 cttctatgtt attggaggcg gttaaaggag gctatatttc aaaagacgga aaagttcgtt
    11941 tactcaaaaa agctgaaggt tggaaaattt actatgagtg agactaaaat acgttgtgat
    12001 tggtgtaaaa gcagtgaaat ttatatgaaa tatcacgacg aagaatgggg aaaaccggag
    12061 tttgacagcc tcaaactgtt tgagaaaatc tgccttgaag ggcaacaagc cggactttct
    12121 tggattacgg tgttgaaaaa acgggaagcc taccgccaag cattccatca gtttaatcct
    12181 gaaaaaattg ccaaaatggg tgaagaagaa attgatttgt taatgcaaaa cactagctta
    12241 atccaccacc gagcaaagtt agaagccatt ataaaaaatg cgaaagctta tatcacaatg
    12301 cagcaaaatg gcgaagactt cagtcggttt atttggtctt ttgtgaatta tcagccgcaa
    12361 attaatgatg taccaacact tgaagctgtt tctgctcata cagaaacctc aaaagcaatg
    12421 ccaaaagcgt tgaaaaaacg tggggttgtg tttgtaggcg agactacttg ctatgctttt
    12481 atgcagtcaa tggggctggt taatgaccat gtgaataatt gcgtgttcaa acataaataa
    12541 acacaaaaaa ttaggcgacc atttggtcgc ctaa
//
LOCUS       DANKBV010000064        12262 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.064, whole genome shotgun sequence.
ACCESSION   DANKBV010000064 DANKBV010000000
VERSION     DANKBV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12262
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.064"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(21..317)
                     /locus_tag="PWY43_001471"
     CDS             complement(21..317)
                     /locus_tag="PWY43_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL2136343.1"
                     /translation="MTAITIPQFEEVTSKQFQSEFGRIVDIVKGKTPVAITQYGRPTM
                     VLFPYDQAVEARRLLAKQRLLEMLAERDKNPPAALAEITLEELDKMIDEERNAL"
     gene            673..2391
                     /locus_tag="PWY43_001472"
                     /pseudo
     CDS             673..2391
                     /locus_tag="PWY43_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249111.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2376..2600
                     /locus_tag="PWY43_001473"
     CDS             2376..2600
                     /locus_tag="PWY43_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2136344.1"
                     /translation="MLRFVGGYMKKYSNDSLINSIVISKLKAGWKIRKGKKHNILIAP
                     NSRRLAIPSTPSDINAYKNFRSKLNHLIAF"
     gene            2756..3484
                     /gene="ttrB"
                     /locus_tag="PWY43_001474"
     CDS             2756..3484
                     /gene="ttrB"
                     /locus_tag="PWY43_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrB"
                     /protein_id="HDL2136345.1"
                     /translation="MDLSKRSFLKELSALTVGASFVPLQSAQAFMPAWREGDENKRYA
                     MLIDLRKCIGCQACTVSCSVENATPLHSFRTTVRQYEITNGTQVVNNVVLPRLCNHCD
                     QPPCVPVCPVQATFQRKDGVVVINNEQCIGCGYCVQACPYDARFINEETKTADKCTFC
                     THRLEVGLLPACVESCVGGARVIGDLNDPKSQISQLYQAHKDDLKVLKPEAGTVPHVF
                     YVGLDDDAFVSKIDGQPMLWTGEA"
     gene            3486..4517
                     /gene="nrfD"
                     /locus_tag="PWY43_001475"
     CDS             3486..4517
                     /gene="nrfD"
                     /locus_tag="PWY43_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HDL2136346.1"
                     /translation="MIREVLVEPQHIVWLPWIVHYFFFVGVAATAVFTAVLFAKKSLK
                     PTACELAAVTVALIGSIVAPVALTADLHQPSRILHFYTDFAWWSPMAWGAIILPLFSV
                     AVAGYFVLALAHHTRPNLPKWLAWLQLPILQNQGLLWAFRLFATLTAIGIIGYTVLET
                     YQTGTRTLWHSAWLLPIMLFSAWAVALGLTQAVCKMLFHTQTNTGLWTKLCLILTALS
                     LVGLAFSSPQATQDFAQLFNGSITAYLVGIFWLIALICNFSAKNHRLQWLGVLALIAF
                     GWLLRWVLVIQVQTIAKTNALQNPYHFDWTAVDGGLGIVSIFGLTVLVSIVVWQLILI
                     ICGGRKGEQ"
     gene            4507..7602
                     /gene="ttrA"
                     /locus_tag="PWY43_001476"
     CDS             4507..7602
                     /gene="ttrA"
                     /locus_tag="PWY43_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrA"
                     /protein_id="HDL2136347.1"
                     /translation="MNNKRRNLLKGGLALGGAAAFGAGYSPKVSEIAKGVMHGTSGEK
                     TRDAIHGNSLTPEYQVKDGKLLSNPEQIVCNTQCMGCWTQCGIRARVDLNTNQVIRIS
                     GNPYHPLSSDKTLPFGMPIAKAELLLAGESGIENRSTACARGAAFLEGVNSPYRITQP
                     LKRVGKRGEGKWQTISFEQLVEEIVNGGNLFGEGEVEGLKTIRNLVDPINPKQPDLGM
                     KANQLLVTFAGPEGRQPILQRFAQKSFGTINFSNHGAYCGASYRAGSGAFMKDFDNQQ
                     HAKPDWDHAEFILFIGTSPAQSGNPFKRQARQLAKRRPDDNFSYVVVSPRLEMTSSLA
                     TQNNHWVPIKPEGDLALVMGMLRWIIENERFNADYLSRPSLAAMENAGHVSYANASHL
                     IICEPNHPKFGQALRISDLQDVQLDPEEKVEENLIVVKDQASGELISAKDCLQAVIFV
                     EQIAKLLDGSEVLVKSSMQLLKEATQEKTLAEYSDICGVPVQTIENLAKKFTSHGHKA
                     NAITHGGTMHSTGFYTSWAILLLNAMVGNLNKKGGMAVAAGKFKDFGDGPRYNLANFP
                     NEVKPKGTNLARSKKAYQNSSEFKQKVTKGENPYPAQGAWYPFTGGQTTEMFTSMVQG
                     YPYFAKAWINHMANPVYGLTAMNPIALEKLKDPKILPLFVSIDAFMNETTALADYIVP
                     DTHNFESWGFSTAWGGVPQRTSTARHPIVPSKNAKTAEGDTICMENFVIAVAKKMGLP
                     GFGENAIADLEGNRYPLHRTEDYFLRAAANIAFAGEKPAPDASEQDLLLTGVQRIMPK
                     IEQTLKAEERLKVANVYCKGGRFAPHESAWKEENMEFQWKNCLQIWNENVYKAQHHSN
                     GEHYWGCPRYMSPRFADGSTLEQHYPQSEWGFKLISFKSNIMSSITAPLLRLLSIKPE
                     GLVAMNRLDAEEKGIKHGDVVKLSTPTAGLTVQIVVLDGIARGTIGIEHGYGHKQIGA
                     SSYTIDGVEIPANPMIAKGINLNDLGIIDHTKAVKSPWVDWVCGSAVRNGIPAKVEKL
                     A"
     gene            7743..8588
                     /locus_tag="PWY43_001477"
     CDS             7743..8588
                     /locus_tag="PWY43_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2136348.1"
                     /translation="MTFVKKTALSLIATALFATSSFAAEQSQYQHIRNATAKIEYAGK
                     TFLVDPYLAEKDRYLPPNAKKRNPTLDMTVSIDEVLNGVDAVIVTHTHHAHWDDVAQQ
                     RVPKNLPIFVQNAGDARLIRSQGFTDVRVVGKDTPFEGVTLTRTRGQHGTDAMYANPK
                     IAEMVGDAMDVVMKAEGKPTVYLVGDTIWNHEIEHSFNTYKPDFVVLNAGCAKLPNFE
                     GSPIMCTEDVSKAHKIAPQAKILTVHMDALPQTSISSDQMRDYVQKEGLNRVTVPKNG
                     DVIKF"
     gene            complement(8701..10098)
                     /locus_tag="PWY43_001478"
     CDS             complement(8701..10098)
                     /locus_tag="PWY43_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL2136349.1"
                     /translation="MKLSKLAFYVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
     gene            complement(10188..10811)
                     /gene="gmk"
                     /locus_tag="PWY43_001479"
     CDS             complement(10188..10811)
                     /gene="gmk"
                     /locus_tag="PWY43_001479"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2136350.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGHFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            complement(10924..11397)
                     /gene="gpt"
                     /locus_tag="PWY43_001480"
     CDS             complement(10924..11397)
                     /gene="gpt"
                     /locus_tag="PWY43_001480"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2136351.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            complement(11542..>12262)
                     /locus_tag="PWY43_001481"
     CDS             complement(11542..>12262)
                     /locus_tag="PWY43_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL2136352.1"
                     /translation="SKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSISVSPNYSYRT
                     VAYRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTA
                     SRYNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERW
                     ATQASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDS
                     NIADFYPRQNKEVFFTLEKLF"
BASE COUNT         3539 a         2660 c         2712 g         3351 t
ORIGIN      
        1 tcgtatcaat cactaaataa tcagagtgca ttacgttcct catcaatcat tttatccaac
       61 tcttcaagcg tgatttctgc aagagctgcc ggcggattct tatctcgctc tgcaagcatt
      121 tcaagcaaac gctgttttgc taataatcgc cgagcttcaa ccgcttgatc gtaaggaaaa
      181 agcaccattg tcggtctgcc atattgggta atcgccacag gggttttgcc ttttacgata
      241 tcaacaattc tgccgaactc agattgaaat tgcttagaag tcacctcttc aaattgaggg
      301 atagtaatcg ctgtcatttt taagctccta atgtgagtga ttttggatat gttagctatg
      361 ttttgtgtaa aaatcaatcg agccaattaa gataaatttt tgtagttatt gtaaattcaa
      421 taacataaaa acttgaacaa acacttgatt ttctattttt tatggcttaa tgtagtaggg
      481 catattatat gtaggttcta tctccaggcc tgcggtcagg tatccatact tgccttgaac
      541 aaaagagata attcgataga ttatattgct tatagtcaat tcatttataa attaaatcct
      601 tttatttcct agtatggctg tgattttcac agaggcattt ttctaactta attaacaaag
      661 aggatttaat atatgtcaaa aaacacccat agctatttac ctttacaaaa ataccatatt
      721 ctaccaattt atggtatttc cccagaatta attaaaaaat ctgttaaaga ttgttctaga
      781 gatagagaaa aaatttctca ctgtactttg ttaaatgctt gctcaaaagc tttaggattt
      841 tctggagact ttgcagggtt tcaagaagat tatttatcta atttgacgca attcatggaa
      901 aaacatgggc tcttatatct tgtagattta acaaaacaaa aatatccagt aaatgatatg
      961 tgcatgacaa ggagcttagc tctgaaaaag caagatatat ctgaaagaat tttctttagc
     1021 ggaaaggaaa tccctaagag aatatttact ggatttaatt tccgatatga tatgcattta
     1081 gctaatggtt ttcgtatcgg aaattcctta aaaaccccat attctgacat aggtatatgt
     1141 gaagacataa atgaagcatt aaaaaatcca tcaataaaaa ttgattgccg tgatggcaca
     1201 atcagaagta tggcagatgt agtcataggt agatatcttg ataaagtatt gttttattat
     1261 tttaacttga taggtgatca gttagtccag cctagattgg ataaatatga aatacaatgt
     1321 tattaccctc aaggttatca aggggatatt aacaatgact tagctctaca taagaaattt
     1381 ttagattttt ttgtatcaag aatttaattt ttagataaag gatgggtaga tgtaataccc
     1441 tataatgata atttggtctt tcttaaagga gaaaatggtg aatatgattt tgtatataaa
     1501 aatcagagaa gtgaactttt tgaacaccaa atatattccc catttttaaa acgctctgac
     1561 ataccatact ttgatgatga atatcatttt aaaagatggt tttattttga atatcaagga
     1621 tttagaagag agctatcaca cttatcagaa attcactttt ataaaaatgg aggagatgtc
     1681 caaaactatc ctacgagaga gtttgattta attaagaaat atttaactaa taaagggatg
     1741 tacacatccc ctcaaaagaa gaacaatgaa gaattaagtg ggtttaataa aattaaaatt
     1801 agtaattgta aaagcattat ggtatcaaat ctaatcacca ttaatgattg gagtatattt
     1861 tgtaaggaaa atcaagatta tattaaaaac agaaagggta tggatgattt aaaatccatt
     1921 aatacagata atgataacat gcctgtttca ttgacttttt atgatgtatt aaaatatata
     1981 aattggttta atcaaaaaca taatgcaaat gtaaggctat taaattttga tgaatataag
     2041 tttatttctc cgtttgaaca aacagataga aatgaatgga agcatgaaga tatagaattt
     2101 atatatgatg agaaaatatc tacagaacca cctccttata tggaggaaga aaaatttcaa
     2161 aaaataataa tgaggttttc aaaagatata aaaataatga tgcacaacca cataaacttc
     2221 atagaatctg acagatttgc ggaatggatt cttgaaaagg cttgtgttcg ctctaaaaca
     2281 ttaaccagct tttatggcga caagtctgtt ataagggccg ttccaccatt ggattgttca
     2341 ggaaaatata aatataccaa aataggattt agattatgct acgatttgta gggggatata
     2401 tgaagaaata cagtaatgat tctcttataa atagtattgt tatatctaaa ttaaaagcag
     2461 gatggaaaat tagaaaagga aagaaacata atatattaat tgccccaaat tctaggagat
     2521 tagcgatacc tagtaccccg agtgatatta atgcgtataa aaactttaga tctaaactaa
     2581 accatcttat tgctttttaa ttagtatcaa tgccctatta tctacttgta tagggcattt
     2641 cccttcatcc ttgacccaaa tcaactttcc ccttgatatg tggaaaacca caataccttg
     2701 ccgttccctc tccgccttag aatacctttg ttatttttaa atttgtgggg gaaatatgga
     2761 tttaagcaaa cgttcctttt tgaaagagct ttccgccttg acggtggggg cgagttttgt
     2821 gccgttgcaa tcggcccagg cgtttatgcc ggcttggcgt gagggcgatg agaacaaacg
     2881 ctatgcgatg ctgattgatt tgcgtaaatg tatcggctgt caagcgtgta ccgtgagctg
     2941 ttcggtggag aacgccacgc cgttgcacag tttccgcacc acggtgcgtc aatatgaaat
     3001 caccaacggc acgcaggtgg tgaacaatgt ggtgttgcct cgtttgtgta accattgcga
     3061 tcaaccgccg tgtgtgccgg tctgcccggt gcaggcaacc tttcaacgta aagacggtgt
     3121 ggtggtgatt aacaatgagc agtgcatcgg ctgtggttac tgcgtgcaag cctgcccgta
     3181 tgatgcccgt tttatcaatg aagagaccaa aaccgccgat aaatgcacct tctgcactca
     3241 ccgtttggag gtaggtctgc tgccggcgtg cgtggaaagt tgcgtgggcg gggcaagggt
     3301 gattggcgat ttgaacgacc cgaaaagtca aatttcccaa ctttatcaag cccacaaaga
     3361 tgatttgaaa gtcctgaaac cggaggccgg caccgtgcca cacgttttct atgtggggct
     3421 agacgatgat gcctttgtga gcaaaattga cgggcaacca atgctctgga cgggggaggc
     3481 gtaaaatgat tcgtgaagta ttagtggaac cgcaacatat cgtctggttg ccgtggattg
     3541 tgcattattt cttcttcgta ggcgtggcgg caacggcagt gtttaccgcc gtgctgtttg
     3601 caaaaaaatc actgaaaccg accgcttgcg agctggcggc ggtgacggta gccttgattg
     3661 gctcgattgt cgcccctgtg gcattgactg ccgacctgca ccaaccgagc cgtattctgc
     3721 atttctacac cgattttgcg tggtggtctc caatggcgtg gggagcgatt atcctgcccc
     3781 tgttcagcgt ggcggtagca ggttattttg tgctggcatt ggctcatcat acccggccga
     3841 acctgccgaa atggttagcg tggctacagt tgccgatact gcaaaatcaa ggcttgcttt
     3901 gggcattccg cctgtttgcc acactcaccg ctatcggcat tatcggctac accgtgcttg
     3961 aaacctacca aaccggcaca agaaccttat ggcatagtgc gtggctgctg ccgattatgc
     4021 tcttttctgc gtgggcggtg gcattggggc ttacccaagc ggtctgcaaa atgctgttcc
     4081 acacccaaac caacacgggg ctatggacaa aactctgcct gattttaacc gcactttcgc
     4141 ttgtcggact agcattcagt agtccacaag ccacacaaga ttttgcccag ctcttcaatg
     4201 gctcaatcac cgcttacctt gtcggcattt tctggctaat tgccctcatt tgcaattttt
     4261 ctgccaaaaa ccaccgcttg caatggctcg gcgtgcttgc cctcatcgct ttcggctggc
     4321 tactccgctg ggtactggtg atccaagtcc aaaccattgc caaaaccaat gccttgcaaa
     4381 atccatatca tttcgattgg acagccgttg atggtggttt ggggattgtc tcgatttttg
     4441 ggctaacggt gctagtaagt atcgtggtat ggcaactgat tttaataatt tgtgggggaa
     4501 gaaaaggtga acaataaacg cagaaatctg ttgaaaggcg ggctggcact tggtggggcg
     4561 gcggcgtttg gggccgggta ttcgccgaag gtgtcggaga ttgccaaagg ggtgatgcac
     4621 ggcacatcgg gcgagaaaac ccgtgatgca attcacggta attcgctcac gccggagtat
     4681 caggtaaaag acggcaaact gctcagcaat cctgagcaaa ttgtgtgcaa tacacagtgt
     4741 atgggctgtt ggacgcagtg tggcattcgg gcgagggtag atttgaacac caaccaagtg
     4801 attcgcattt cgggcaatcc gtatcacccg ctttcctccg acaaaaccct gccgttcggt
     4861 atgccgattg ccaaggcgga actgttgctg gcgggcgaaa gtggcattga aaatcgctcc
     4921 accgcctgtg ctagaggggc ggcgttcttg gagggggtga acagcccgta tcggattacc
     4981 caaccgttga aacgggtcgg aaaacggggc gaaggcaagt ggcagaccat cagctttgag
     5041 cagttggtgg aggaaatcgt caatggtggc aacctatttg gtgaaggcga agtggagggc
     5101 ttgaagacga tccgcaatct ggtcgatccg attaacccga aacagcctga tttgggaatg
     5161 aaagcaaacc agcttttagt tacttttgcc ggccccgaag gtcgtcagcc gattttgcaa
     5221 cgctttgccc aaaagagctt cggcacgatc aacttttcca accacggggc ttattgtggc
     5281 gcttcgtacc gtgccggttc gggtgccttt atgaaagatt ttgacaacca acaacacgcc
     5341 aagccggact gggatcacgc tgaatttatt ctgtttatcg gcacgtctcc ggcacagtcg
     5401 ggcaatccgt ttaagcgtca ggctcgccag cttgccaaac gccgccctga tgacaatttc
     5461 agctatgtgg tggtttcccc tcgtttggag atgacctcct ccctcgccac ccaaaacaac
     5521 cattgggtgc cgattaagcc cgagggcgat ttggcgttgg taatgggaat gttgcgttgg
     5581 attatcgaaa acgagcgttt caatgctgac tacctctccc gcccaagtct ggcagcgatg
     5641 gaaaatgccg gccacgtcag ctatgccaat gcgtcacatc tgattatttg cgaaccgaac
     5701 cacccgaaat tcgggcaggc gttgcggatt agcgatctgc aagatgtgca actcgacccc
     5761 gaagagaaag tggaagaaaa cctgattgtg gtaaaagatc aggcaagcgg cgaactcatt
     5821 tcagcaaaag attgcttaca agcggtcatt tttgtcgaac aaattgcaaa actgcttgat
     5881 ggctcggaag tgttggtgaa atcctcaatg cagttgctga aagaggcgac acaagagaaa
     5941 acccttgccg aatatagcga catttgtggc gtgccggtgc aaaccattga aaacttagcg
     6001 aaaaaattca cttcgcacgg tcataaagcc aatgccatca ctcacggcgg cacgatgcac
     6061 agcaccggtt tttataccag ttgggcgatt ttgttgctga atgcaatggt cggtaacctc
     6121 aacaaaaaag gcggaatggc agtagccgca ggcaaattca aagatttcgg ggacgggcct
     6181 cgctacaatt tagcgaactt cccgaatgaa gtgaagccaa aaggcacgaa ccttgcccgc
     6241 agtaaaaagg cgtaccaaaa ttcaagcgaa tttaagcaga aagtgacgaa gggcgaaaat
     6301 ccgtatccgg cacaaggggc gtggtatccg ttcaccggcg ggcaaactac cgagatgttt
     6361 acctcaatgg tgcagggcta tccatacttt gcgaaagcgt ggatcaacca tatggcgaac
     6421 cccgtttacg gcttaaccgc tatgaacccg attgcgttgg agaaattaaa agatccgaaa
     6481 attctaccgc tctttgtctc tattgatgct tttatgaacg aaaccactgc actggcggac
     6541 tacatcgtgc cggacaccca caactttgag agctggggct tttcaacggc gtggggcggt
     6601 gtgcctcaac gcaccagcac cgcacgccac ccgattgtgc cgtccaaaaa cgccaaaact
     6661 gccgaaggcg acaccatctg tatggagaat ttcgtgattg cggtggcgaa aaaaatgggc
     6721 ttgccgggct ttggcgaaaa tgcgattgcc gatttagaag gcaaccgcta tccgctccac
     6781 cgtaccgaag attacttcct acgggcggca gcgaacattg cctttgccgg tgagaaacct
     6841 gcaccggacg ccagcgagca agatctgctg ctcaccggcg tgcaacggat tatgccgaaa
     6901 attgaacaaa cgctaaaagc cgaagagcga ttaaaagtgg cgaatgtgta ttgcaaaggc
     6961 ggacgttttg ccccacacga aagtgcgtgg aaagaggaga atatggagtt ccagtggaaa
     7021 aactgcctgc aaatttggaa cgaaaacgtg tataaagccc agcaccacag caacggtgaa
     7081 cactattggg gctgccctcg ttatatgtcg ccacgttttg cggacggctc cacgctggag
     7141 cagcactatc cgcagtcgga atggggcttt aaactgattt cgtttaaatc gaacattatg
     7201 agcagtatta ccgccccact gttgcgtctg ctctcgatca agcctgaggg cttagtggcg
     7261 atgaaccgtt tagatgcgga agaaaaaggc attaaacacg gcgatgtggt gaaactcagc
     7321 accccaaccg ccggcttgac ggtgcagatt gtggtgttgg acggtatcgc ccgaggcaca
     7381 atcggcatcg aacacggcta cggtcataaa cagattgggg caagttccta caccattgac
     7441 ggcgtggaaa ttccagccaa cccgatgatc gccaaaggga ttaacctcaa cgacctcggc
     7501 attatcgacc acaccaaagc ggtcaaatcc ccgtgggtag attgggtctg tggctcagcg
     7561 gtgagaaatg gcattccggc gaaggtagag aaattagcct agccgaacaa gcggttggtt
     7621 ttgcagaaaa atgtgcaaaa tcgaccgctt ttattgatgt aagggacact gttccttatg
     7681 caaaccagca ttgcagagcg taaaaaatca tactctgcat tttaatttat tttggagaac
     7741 ctatgacttt tgtcaaaaaa acagcactga gcctgattgc aacggcactt ttcgctacat
     7801 caagttttgc cgctgagcag agccaatacc aacatattcg taatgcaacg gcgaaaattg
     7861 aatatgccgg caagacgttt ttggttgatc cgtatttagc ggagaaagat cgctacttac
     7921 cgccgaatgc gaaaaaacgt aatccgacct tggatatgac tgtgagcatt gatgaagtgt
     7981 taaatggcgt ggatgcggtg atcgtaactc acactcacca cgctcactgg gacgatgtag
     8041 cacaacaacg tgtgccgaaa aatctgccga tttttgtgca gaatgccggt gatgcccgtt
     8101 taatccgttc acaaggcttt accgatgtgc gtgtggtcgg caaagatacc ccgtttgaag
     8161 gcgtaacctt aacccgaact cgtggccagc acggcaccga tgcgatgtat gcgaacccga
     8221 aaattgccga aatggtcggc gatgcgatgg acgtagtgat gaaagcggaa ggcaaaccga
     8281 ccgtctattt ggtgggagat accatttgga accacgaaat cgaacattcg ttcaacactt
     8341 acaaaccaga ctttgtggtg ctgaatgccg gctgtgccaa actaccaaac tttgaaggca
     8401 gcccgattat gtgtaccgaa gatgtttcca aagcacacaa aatcgctcca caagcgaaaa
     8461 tcttaactgt gcatatggac gccctgccac aaacctcaat cagcagtgat cagatgcgtg
     8521 attatgtaca aaaagaggga ttaaatcgtg taactgtgcc gaaaaacggt gatgtgatta
     8581 agttctagta gctaaactaa cattaccaaa atggtttaag tagcaataaa tagcgaagcg
     8641 ggttaagcca tcaagcctta acccactttt accaaccata gtatatagtt ttagatataa
     8701 ttatcttctg tatttacctg ccattgcttg gtaaaccgca ttctcgttgg tcaaaatcgc
     8761 atattttgca tttaaaatcg ccaactgaga attaagctcg gtattcagtg cggtaatcca
     8821 ctcacggaac tccgacaccc cttgttcata acggttttta tagtatgaac tgatacgttt
     8881 atttttcgca ttggtatctt gaaggatttt taacgagctt ctcgactgcg tataactgta
     8941 ataataacgg tcaatttcat ttaaggctga ggtaattttt tgctcatagt ttaaacgggc
     9001 agtcacataa tccgtttcag aaatttttac gttattttta acccgattcc aatctaagaa
     9061 cggtaaatcc aatttaatta agccgttacc cacagggtta tctgccacat tgccgatatt
     9121 ggttgcacta ccggtaatag aaccaccgat ggttaaggtt gggaaccagc ttttctcggt
     9181 tgcggttaag gttttaaatg cactttgtaa gcgttgtaac gagcctacta catcagggcg
     9241 attagcaatg gctgacactg gcacatttaa atctacccct tgcaatttca cgctcataat
     9301 attcgggtaa cgaatgttaa gcggagtatt tggctgaaca tttaacaaat tgcgtaatgt
     9361 ttgctcggca attttttggt tggttttcag atcaattaca ctatttctgg cgtttaagac
     9421 cgcttgttga gcctgatcaa ccgctaaact atcaatcgca ccggcattca ttttgttagt
     9481 taaaatacgg ctgatttgct cataatggcg aatgctctct tcggtaatac gaatcgcatc
     9541 acgatagtaa gctaactggt agtaagtagt cacaaccgca ttaataatag ataaacgggt
     9601 agcttttaag tcttcaatgg tggctttatg cgtccattct gccgcactgg cggtatcggc
     9661 taaacgtcgc cataaatcca cagtataact taaattaaat gcagcttgag ctgcggttgt
     9721 tgaaacaccg gtcgaaacat tatttgaaga agccgaacct aaacctttag ccgcagaaac
     9781 agaacctgaa ccactaaagg ttggcactaa atttgcccct actaaattag cgttatacaa
     9841 ggctttattg actgcaattc cgcttttggc taaatcaagg ttatttttga tggctaaatc
     9901 aaccacacgg tttaattccg catccgtata gccttgccac cattgctcgt taatttgata
     9961 ttgtttggta atttcatcat agctttgata ggtttgctgt gcttgttgca aagtcccgtc
    10021 attgctcata ttccctgtgg tacaagccgt tacggttaga gccaacgcca cataaaatgc
    10081 taattttgaa agtttcatta catttccttt tgaaaataaa gaggggaaaa tgggcgtatt
    10141 ctacccaaaa acagattgcc tttccagtta aatgaacaac tgttcattta attatttaac
    10201 aaatcagtaa tcaaatgctg atgacgaatt tgttgcgaac ctaagctcaa acgctcggca
    10261 attaaaatcg cttttagctc tgccaatgcc tgttggaaat catcattaat aatcacataa
    10321 tcgaactcat tgtaatggtt catttccgac accgcctctg ccatacgttt ggcaatggtt
    10381 tcagcggaat cttgcccacg cccgattaaa cgtttttcca attctgtttt tgacggtggc
    10441 aaaataaaaa cggttttcac attcggcact ttttcccgaa tttgtctagc cccttgccag
    10501 tcaatatcca aaaagacatc aatgccctgc tctaggctac gttcaatcat cggcaatgat
    10561 gtgccgtaat aattgccaaa cacctccgcc cattctaaaa aatgcccttg ctcaatcagc
    10621 tgcttaaact catcgtgcga agtgaaataa taatgcacgc cgtcctgttc tgccggacga
    10681 ggctgacggg tagtgtgtga aatcgaaagc tgtacttccg aacggggtaa atccgccaac
    10741 aaggcgttaa ttaaggaaga ttttcctgct ccacttgggg cagaaataat ataaagattg
    10801 cctaaactca ttatttgtat atacctattt gattttggga atagggcgaa tgatgattcg
    10861 cccctacatt tcataggaat aaataaaaaa taatggacaa ctttcattgt ccattacaca
    10921 aaattatcta cgtgccaaag gctgaacgaa ggtaatgcct aaatcccaag gctgctcaat
    10981 ccacgtttct tgtggaatat caatcacata atcatcaacc aacggtgcac cgtgcggttt
    11041 cgcaaacacg gttacaaaac gagctttcgg gtacattctg cgaatttctt ttgccgtgtt
    11101 gccagtatcc actaaatcat ctaccacaat aaagccttcg ccatcggtat cggctgcgtg
    11161 taacacattt aactcacctt ggctatcgtg atcgtagctg gcaatgcaca ctgtttctac
    11221 gtggcgaata cccaactcac gtgctaaaac ggctgccgga aacaagccgc cacggctcac
    11281 cgcaataatc cctttccact gtgtggcagg taataaattt tctgccaact tgcgagcgtg
    11341 catatggaac atatcccacg tcacgacata tttttcattt gtgtattttt cactcataga
    11401 aacaacctaa tataaaattg ggggctaccc cgatataaaa aatcgcacta ggataacccg
    11461 aaaatgcttt tttcgccatc atttcattta ttttttgtgc cggcagctac gcaatcgtat
    11521 gcttagcaca aaaaaccaac actaaaatag cttttctaaa gtgaaaaaga cttccttgtt
    11581 ttggcgaggg taaaaatcag caatattgct gtcaattttt tgataacgga aattcagctt
    11641 cggcacaatg ccctgccaat gccaagtgcg gttccaaacc gctaaattgg cttgatattc
    11701 cgtgtctaac cgcttatagc caaacaaata gtgcttgtcg gcaaaatatc gcttgccaaa
    11761 gccgatactt gcctgcgttg cccagcgttc gccttgaaaa accgccccca ctcgagccag
    11821 ccacttattc gagctttgtg ctttttcccg tgcaatttca cgcacgccgt ctaaatttgc
    11881 aaaaaaccgc ccatttttga ccgcttgcca ctgcacaccg ccactgagtt ggtgctgata
    11941 gctgttataa cggcttgcgg ttagctcctc aaaataccgt ttttgcaaat gggtaaaact
    12001 ccccgacacc aaccattgcg aattaaggta acgcaaaccg ttgagtgttg ccccaaattg
    12061 aacattataa cggctactcc ccagccaatt ttgctcaata aaaggcgaaa aagtcagccg
    12121 ataagcgacg gtgcggtagc tataattcgg cgaaacggat atggattgct cacggtaatc
    12181 tcggttatcc caatagtaaa tcccgctata attgagcgtt aaaccgacaa aatgattgcc
    12241 tttcacgttg tgcaatttac tc
//
LOCUS       DANKBV010000065        12038 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.065, whole genome shotgun sequence.
ACCESSION   DANKBV010000065 DANKBV010000000
VERSION     DANKBV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.065"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1360
                     /gene="ligA"
                     /locus_tag="PWY43_001482"
     CDS             <1..1360
                     /gene="ligA"
                     /locus_tag="PWY43_001482"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2136353.1"
                     /translation="PLVLNAYGIGVYESDDELPATHFERLQWLKSLGIPVNNEIRLVQ
                     GKAALLAYYAEIQAKRPTLGYDIDGTVLKVNDIALQEQLGFISRSSRWAIAYKFPAQE
                     EMTILKEVDFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVII
                     RRAGDVIPQITGVVLERRPENAKKIEFPTACPICHSAVTRVEGEAVARCTGGLFCEAQ
                     RKEALKHFVSRKAMDIDGVGEKLIEQLMARELIETPADLFKLDHTTLMRLERMGEKSA
                     QNALNSIEKAKNTTLPRFLFSLGIREVGEATALNLANHFGSLEAIRQATFEQLKEVQD
                     VGEVVANRIVSFWQEPHNVEVVEDLIAQGVRWENVVKAEIVDNPLKDKNVVLTGTLTQ
                     LTRDEAKALLQSLGCKVSGSVSSKTDYLIAGEKAGSKLAKAQELGIQILSEQQFIELT
                     QH"
     gene            complement(1423..2691)
                     /locus_tag="PWY43_001483"
     CDS             complement(1423..2691)
                     /locus_tag="PWY43_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL2136354.1"
                     /translation="MTQQIEHSTWQSKFAAMGPGILMASAAVGGSHIIASTQSGAIYG
                     WQLAIIILLANLFKYPFFRFGVQYTLASGKTLLEGYKEKGKFYLIIFFILNVFAAMIN
                     TAAVAIVSAAILKFIFSALGIPLDLSIPMAGTIVIVVTWGILLLGQYKFLDTLSKWIM
                     IALTISTVSAVIIAALKGGVEIAPDFVEPSPWNLAALVFIVALMGWMPAPIEISAINS
                     MWVIAKKRLSKVNYEDGIFDFNVGYIGTAILAIVFLALGALVQYGSGEKVEQAGVAYI
                     AQLIKMYAFAIGDWSKLLIAFIAFMCMFGTTITVIDGYSRANAEALRLISGRKQHSQT
                     ELSIWMTVTALIGILIISQFMSDVAKMLQFAMISSFVSTPVFAWLNFSLVLKGEHRAK
                     GWLFWLAVIGLIYLTSFTLLFLAQQFKLIG"
     gene            complement(3015..3725)
                     /locus_tag="PWY43_001484"
                     /pseudo
     CDS             complement(3015..3725)
                     /locus_tag="PWY43_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247995.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
     gene            complement(3694..3840)
                     /locus_tag="PWY43_001485"
     CDS             complement(3694..3840)
                     /locus_tag="PWY43_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136355.1"
                     /translation="MIKPLDSVYWRGKDVSEFFARKRLPDTAVQCVKDNELSYVYGYS
                     TSFV"
     gene            complement(3958..5175)
                     /gene="fabB"
                     /locus_tag="PWY43_001486"
     CDS             complement(3958..5175)
                     /gene="fabB"
                     /locus_tag="PWY43_001486"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDL2136356.1"
                     /translation="MKRVVITGLGVISSIGNNKEEVLESLKAGKSGIEFVPEFAEVGM
                     RSQVAGTIKLNPAELIDRKIYRFMGDAAAYAYLSMKEAIEDSGLTEEQVSNDRTGLVI
                     GAGTGSAHSQLVACDAVRGPRGVKAVGPYAVTKTMASSVSACLATPYKIRGVNYSISS
                     ACATSAHCIGHAMELIQLGKQDMVFAGGAEELSWECATEFDAMGAVSTKYNETPTKAS
                     RAYDANRDGFVIAGGGAVVVVEELEHALARGAKIYAEIVGYGATSDGYDMVAPSGEGA
                     ERCMRQAMATVNGEIEYINVHGTSTPVGDVKELGAIRNVFGDKTPAISSTKSMTGHSL
                     GAAGAHEAIYSLLMLDNNFIAPSINIETLDEQAQGMNIVTERQDKALTTVMSNSFGFG
                     GTNACLVFQRYSK"
     gene            5436..7973
                     /locus_tag="PWY43_001487"
     CDS             5436..7973
                     /locus_tag="PWY43_001487"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL2136357.1"
                     /translation="MKTLTLTANKITAVEGAAIGANQGSFAQKLNSTKARIDQLNAYR
                     IERAINANNHHFMHVFSLIPLLIHLNHPVLPAYVENAPQGIWHFQLSDYQKQFLVSYR
                     FDEAISADHLGPHPSFDALYSMGSTGTITQTSCSDLDLWLCYPNHFTIEQYKLMELKI
                     NKLQEWAKTFQININIYLMNPEQFKSQTYNSSVTDEHSGSAQHFFLLDEFYRSAILLA
                     GKRLLWLHLHKNEYQNVHNNPEIDLTEWIDFGDFSSLSTSEFFGASLWQLYKGINNPY
                     KSAIKILLLECYAHTYPKTKLISKEFKKKLLSDNALEYHFDPYLAMLELVTEHLRSRK
                     EWVKLDRLRVCFYAKAVEGEINNNWRSRDLDELVNQWSWSEKQIQLLQNRQHWKIKQV
                     MNHENMLIDHLLQSYRNLIHFARKFHINPSIMTNDTDILMRKLYSVFEALPGKISLIN
                     HKISSNLAEKAITFIEAKVGSALQPGWYLVNQAPKSRYDSENRYVHYNKSPIKLIAWG
                     YFNGIINAATNLHTVSPNLDIEKLRQFITDLRLSFPIQAPEVTNDDMHHPNEIRNLVI
                     ALNLVQDPTEHIDELPKKVYPSDLFNFGKAQKTLVGSVSIIYRNVWNEIRTQHFDGED
                     AILKALKLVSNRIYQENSSPQSVNVFCYSKYLRSELRDFVADLVHKCITIQTGCLLQE
                     NEFSTLKVAGKTWQFLFHKKTFSIKQIDEQQAVENLNSFAKTKQNAKSENEKKKEFPQ
                     QISEFASEGFLQFFFEDNVDNSFNVYVVDERNNVESYYNCFGNKEEKIRKINKLQNEK
                     QLKTETPASFGSFNHPQFYQLISPNGELCIVPFQSRQHLDYLAQKHH"
     gene            8004..9872
                     /locus_tag="PWY43_001488"
     CDS             8004..9872
                     /locus_tag="PWY43_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2136358.1"
                     /translation="MSGLVSPELIKTVVLLATSVTIVPLFKRLGLGSVLGYLVAGCLI
                     GPSGIGLFQDPAAVVHMAELGVVMFLFIIGLEMHPELLWAMRKAIFGRGFLQVAICGT
                     LLTLAGIYLLGFSKEVAFIAGMGFTLSSTAIVMQVLEDKGISSTPKGQRIVATLLFED
                     LAIVPLLASVAFLAPNQVDAESSTNWSAIGLSLVAVLILVASGKWLMNPIFRVISKAK
                     VREMMTAAALLVVLGSALLMEISGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGLL
                     LGLFFMGVGMSLDLSLVLNNWLWLLVIVAVYVIGKGLGIFIAASITRLTLNESIVRTT
                     IMAHGGEFAFVLFSAAATAGILTPDTHATLTAAVIVSMLLSPLLILIKQGVRKKKAKN
                     ESTNMTGVELPEDLEANVLVIGFGRFNQIVCQALLAKGLSVSVIDSNTDHIRAAARFG
                     FKLYYGDGARLDVLRASGIANANCVIVGVGNPERTEKIVELIKSEYPLVPVFARTYDR
                     QSAVDLVKKHHVDYHIRETFESALALSQAAIERLGSSEEEAKQIISYVRRLDAERFNE
                     ELLHGFSAEVIRKYWVPTPWVNPQHEGSALNEETADILEELGETVEVIKVDITDKN"
     gene            complement(10032..10928)
                     /gene="malM"
                     /locus_tag="PWY43_001489"
     CDS             complement(10032..10928)
                     /gene="malM"
                     /locus_tag="PWY43_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDL2136359.1"
                     /translation="MKKTLLSTAIFFAHLLVVFPSIANGSAGKSIHINSAELAQIQWQ
                     DVPFSQTVKTDLSEQHKQAFTTAFAGIASPVAAYRIPANQGTLEIEIESPVMDKNVFV
                     PTAVVLDSQFNVASTYPSSEFKLLEERGLKGSRLGAELNLTPAMNQDYIYLLIYTTQQ
                     DLAKTTMMPHPAKTYAKATGKQPPAINDIEVAHSLKGQIHINVTGSNGTKFIGLPTEI
                     FSSNKAATPAAKTAIPLPIKENPVAVKTAVDKDTEAYFNQAVMKAIKAKDINKAMNLV
                     NEAEKLGLTSPRQIFLKNVSSN"
     gene            complement(11010..>12038)
                     /locus_tag="PWY43_001490"
     CDS             complement(11010..>12038)
                     /locus_tag="PWY43_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin"
                     /protein_id="HDL2136360.1"
                     /translation="GGTLHNNDWTPTSPALRELNVQFKNFADSLPGATLWAGKRFYQR
                     HDVHMNDFYYWDISGPGAGVENIDLGFGKLSLAVTRDTERGGANTFGYTTVYKLDPST
                     GKIKLDKQRNKADVYNDIFDVRLAGIELWKDGSLELGFDYGNAHAKKGAALEKGATKN
                     GYMVTAEYTQGNFFGGFNKFTAQYATDSMTSWNNGHAQGSKADNKGNMLRLINQGVVQ
                     ASDKVEVMYALIYEKTKLDNKQGKTWYSAGIRPMYKWNDTMSTLLEVGYDRIKDQATG
                     KKNDLVKYTVAQQWQAGNSIWARPAIRVFGTYARWNDKFNTKARTDAGYKAKDGEFIT
                     GVQFEAWW"
BASE COUNT         3729 a         2455 c         2237 g         3617 t
ORIGIN      
        1 tccgttagtg ctgaatgcct acggaattgg ggtgtatgaa agcgatgacg aactgccagc
       61 cacccatttc gaacgtttgc aatggctgaa atcgcttggt attcctgtaa ataatgaaat
      121 ccgcttggtt caaggcaaag ctgctctctt ggcttattat gcggagattc aggctaaacg
      181 cccaacatta ggctatgata ttgatggcac agtattgaaa gtaaatgaca ttgccctaca
      241 agagcaactc gggtttatct ctcgctcctc tcgttgggcg attgcgtata aatttccggc
      301 tcaagaagaa atgacaatcc tcaaagaagt ggacttccaa gtgggaagaa caggggcaat
      361 cacccctgtg gcgaaattag agcctgtttt tgtcgcaggc gtaacggtaa gtaacgcaac
      421 gctacacaac ggtgatgaaa tcgagcgact tggtattgtg attggcgata cggtaattat
      481 tcgccgagcg ggcgatgtaa ttccgcaaat tacaggcgtg gtgttagaac gccgcccgga
      541 aaatgcaaaa aaaattgaat ttccgaccgc ttgtcctatc tgtcactccg ctgttactcg
      601 ggtagaaggc gaggcagtag cacgctgtac cggtggttta ttctgcgaag ctcaacgtaa
      661 agaagccttg aaacatttcg tttcacgcaa ggcaatggat attgacggcg taggcgaaaa
      721 actaatcgag cagttaatgg ctcgagagct gattgaaacg cctgccgatc tgtttaagct
      781 cgatcacact accctaatgc gattagagcg aatgggggaa aaatcggctc aaaatgctct
      841 caacagcatc gaaaaagcaa aaaacactac tcttcctcgc tttttattct cgttaggtat
      901 tcgtgaagtg ggcgaggcaa ccgctctcaa cttagccaac cattttggta gcttagaggc
      961 gatccgccaa gccacttttg agcaattaaa agaagtgcaa gatgtgggcg aggttgttgc
     1021 caaccgtatt gtcagcttct ggcaagaacc gcataatgtg gaagtggtgg aagatttgat
     1081 cgctcaaggt gtacgttggg aaaacgttgt gaaagccgaa attgtagata acccgttaaa
     1141 agataaaaac gtggtcttaa ccggcacact aacccagctc acccgtgatg aagccaaagc
     1201 cctattacaa agtttgggct gtaaagtctc cggctcggtt tccagcaaaa ccgattactt
     1261 aatcgccggc gaaaaagccg gttccaagct ggcaaaagct caagagttgg gaattcagat
     1321 tttaagcgag caacaattta ttgagcttac tcaacattaa caaacaaagg ggcagatgat
     1381 tgaaatctgc cccttattct atctattgaa tgcttaaaag cattaaccaa tcaatttaaa
     1441 ctgctgagct aaaaacagta acgtaaagct agttaagtaa attaagccga taactgccaa
     1501 ccaaaataac cagccttttg cacgatgctc accttttagc actaaagaga aatttaacca
     1561 cgcaaaaaca ggggtagaaa caaatgatga aatcattgca aattgtaaca ttttcgccac
     1621 atcactcata aattggctaa taattaaaat gccgatgagt gcagttaccg tcatccaaat
     1681 acttaattct gtttgtgaat gttgcttacg cccgctaatt aaacgcaacg cttctgcatt
     1741 cgctcgtgaa tatccatcaa taacagtgat cgttgtgccg aacatacaca taaatgcaat
     1801 aaatgcgatt aaaagcttag accagtcacc aatcgcaaaa gcatacattt tgattaattg
     1861 agcaatatag gcaacaccgg cttgttctac tttctcacct gaaccgtact gaactaacgc
     1921 acctaatgct aaaaaaacaa tcgccaaaat tgccgtgccg atataaccca cattaaaatc
     1981 aaaaataccg tcctcataat tcactttact taaacgtttt ttcgcaataa cccacattga
     2041 gttaatggca gaaatttcaa ttggtgcagg catccagccc attaatgcaa cgatgaacac
     2101 aagtgctgct aaattccacg gcgaaggctc tacgaaatct ggagcaattt caacgccacc
     2161 ttttaaagca gcaatgatca cggcagatac cgtagagatg gttaaagcaa tcataatcca
     2221 tttagataat gtatctaaga atttatattg ccctagaagt aaaatacccc aagtgaccac
     2281 aatcacaata gttcctgcca ttggaatgct taaatctaac ggaataccta aagcagaaaa
     2341 aataaatttt agaatcgctg ctgaaacaat cgccaccgca gctgtgttaa tcattgcagc
     2401 aaaaacgtta aggataaaga aaataataag ataaaacttt cctttttctt tgtagccttc
     2461 caataaagtt ttaccgctag ctaaggtata ttgcacacca aagcggaaaa acggatattt
     2521 aaataaattg gctaacagaa taataatcgc cagctgccag ccataaatcg caccagattg
     2581 tgtagaggca ataatatgcg aaccacccac tgccgcagat gccatcaaaa tccccggccc
     2641 cattgcagca aatttacttt gccaagttga atgttctatt tgttgtgtca tatagctttc
     2701 cttatcacat tactatcaaa acaagatggt ttcattctac ttataaaaac caaataaaaa
     2761 cactcctcta gaaaagatgt tcataaaata atacagatat tatgctgaat agagagtatg
     2821 aaatgataag aaagcattta taaaaatgat ttaataagaa ttaaaaacta agatagaata
     2881 taaaaatggt tttaaataaa attaaaaata aattgtaaat ttttatttac caagattttt
     2941 agcaaagttt tcactaaaaa tgaccgcttg caggcaataa aaaagtgcgt ttccgcactt
     3001 tttactaaaa tgatttaatt actcaaccaa tgctcaccgt tgccattagc tggtggatta
     3061 atcggttggt tattattctt acctggaata ataatttgag gctgatcgta catacatttg
     3121 ttgttcgaca atatatcaca gggaatagca catacggcag gggcagaaag aagacaactt
     3181 taaccacctt tttgattaca attctctaat gcattttctt cagcaagact actaacgata
     3241 ccatacccaa aatcctcgaa gccttgacca tctttataac cttgtgcaaa agcaaggcac
     3301 atattactaa acgataagat catttggcaa ttacgaccac cgttcttttc acactgttta
     3361 aacgcatctt gcttcgcatt ttctaaattt ttagacggat tatcagtcac ccacatcata
     3421 ttttgttcag gtgacatata aattgatgac cataaacgag gctgtgtttt acgctttgct
     3481 aagtcctctt tactggcaat ggttaccgct tctttatatt caccacattt aaactctttt
     3541 tcgacatcac caaaaccatt catttcacga acagaagtga ttttattatc agcgtcagca
     3601 aaaagatatg tccaagtcga tccacctgta taaaccttat ctgttgtcat aaatatagtg
     3661 gttgcattac gaccaacttc ataagtttgc aaattataca aagctagttg aataaccata
     3721 aacatagctt aattcattat cttttacgca ttgaaccgct gtatcaggta aacgtttacg
     3781 tgcaaaaaat tcagatacat ctttaccacg ccaataaaca ctatctagtg gcttaatcat
     3841 tgtagaacaa gcggtcaaaa ataatgacaa aataacaaat agcaaagaac ttattttgaa
     3901 attcataata attacctcaa aagaaaaaag agcaagcttt cacttgctct ttttatgtta
     3961 cttagaatag cgttggaaca ctaaacacgc attagtacca ccaaagccga agctgtttga
     4021 catcacggtt gttaatgctt tgtcttggcg ttcagtcaca atattcatac cttgtgcttg
     4081 ttcatcaagg gtttcaatat taatgcttgg tgcaataaaa ttattatcta acattaataa
     4141 tgaataaatt gcctcgtgcg cacctgctgc acctaatgag tgtccggtca ttgatttggt
     4201 tgatgaaatt gctggcgttt tatcaccaaa gacattacgg atcgccccta actcttttac
     4261 atcacccact ggagtagaag tgccgtgtac gttaatatac tcgatttcac catttacagt
     4321 tgccatcgcc tgtctcatac aacgctcagc accttcacca cttggcgcga ccatatcgta
     4381 accgtctgag gttgccccat agcccacaat ttcagcatag attttggcac cacgagcaag
     4441 agcgtgttct aactcttcca ccacaaccac tgcaccacca ccggcaatca caaaaccgtc
     4501 acgattagca tcataagcac ggctagcttt tgttggtgtt tcattatatt tagttgaaac
     4561 agcacccatt gcatcaaact cggttgcaca ttcccaagaa agttcttctg caccacctgc
     4621 aaacaccata tcttgtttac ctaattggat taactccatc gcgtgtccaa tacagtgtgc
     4681 tgaagtcgca caagctgagc tgattgaata gtttacacca cgaattttat aaggtgttgc
     4741 caagcatgct gacacacttg atgccattgt tttggttact gcgtaaggcc caacggcttt
     4801 cacaccacgt gggccacgta ctgcatcaca agctacaagt tgactgtgcg ccgagcctgt
     4861 accagctccg atcaccaaac cggtacgatc gtttgaaact tgttcttcgg ttaagcctga
     4921 gtcttcgatt gcttctttca ttgaaagata agcgtaagcc gcagcatctc ccataaaacg
     4981 atagatttta cggtcaatta actcagccgg atttaattta atcgtacctg ccacttggct
     5041 acgcataccc acttcagcaa actcaggcac aaattcaatg cctgatttac ctgcttttag
     5101 tgattccaac acttcttctt tgttgttacc gatactggaa attacaccta atcctgtaat
     5161 aacgactctt ttcattttgt ttccttcatc attttgatct gaaaaatact tgattcagct
     5221 tacacttgtt cactcaaata agctactaaa acttgattat tttacacaat ttcattactg
     5281 gtctgaactc tttctatcaa gatagcggtt gaatttcaga cattttttgc aaaataggga
     5341 atgaaattta atttcccgat tttttaccct attacataat aatcactttt ccgctaaaat
     5401 gctaaacagt tcacacttta gtaaggaaag ctgacgtgaa aacattaacc ctgacagcca
     5461 acaaaataac agccgtagaa ggcgctgcca ttggggcgaa ccaggggagc ttcgcccaaa
     5521 aattaaactc tactaaagca cgaattgatc aacttaatgc ataccgaata gaacgagcta
     5581 tcaatgctaa taatcatcat tttatgcatg ttttctctct cattccattg cttattcatc
     5641 ttaatcaccc tgtgttaccg gcttatgttg aaaatgcacc acaagggatt tggcattttc
     5701 aactctctga ttatcaaaaa caatttctgg tttcttaccg ttttgatgaa gcaatctctg
     5761 ccgatcattt gggcccccat cccagctttg atgctcttta ttcgatgggt agcaccggta
     5821 ctattaccca aacaagctgt tctgatttag atttatggct atgctatccg aaccatttta
     5881 caattgaaca atataagtta atggagctta aaattaataa attgcaagaa tgggcaaaga
     5941 cattccaaat caacatcaat atttatttaa tgaacccaga acaatttaag agccaaactt
     6001 ataacagtag cgtaaccgat gaacacagtg gttcagcaca gcattttttt ctattagatg
     6061 aattttatcg ttcagctatt ttacttgccg gtaagcgttt actctggttg catttacata
     6121 aaaatgaata ccaaaatgtt cacaataacc ctgaaattga cttaacggaa tggattgatt
     6181 tcggcgattt ttccagcctt tcaaccagtg aattttttgg tgcaagctta tggcagctct
     6241 acaaaggaat taataaccct tataaatcgg caattaaaat tttattgctc gaatgctacg
     6301 ctcacactta tcctaaaaca aagctcatct ccaaagaatt taagaaaaaa ctactaagtg
     6361 acaatgcttt ggaatatcac tttgatccct acttagccat gcttgaattg gtaacagaac
     6421 acttaagatc acgcaaagag tgggtgaaat tagaccgctt gcgagtctgt ttttatgcaa
     6481 aagcggttga gggagaaata aataacaact ggagaagtcg tgatttagac gagctggtta
     6541 accaatggtc ttggtcagaa aaacaaattc aattacttca aaaccgtcaa cattggaaaa
     6601 ttaagcaagt gatgaatcat gagaatatgc tgattgatca tttactacaa agttatcgaa
     6661 atttaattca ttttgctcga aaatttcata ttaatccgag cattatgaca aatgatactg
     6721 atattttaat gcgaaaactt tattctgtat ttgaagcact tccaggaaaa atatctttaa
     6781 ttaatcataa aatatcgtcc aatttagcag aaaaagcaat tacatttatt gaagctaaag
     6841 ttggatctgc gttacaaccc ggttggtatt tagttaatca ggcacctaaa agtcgttatg
     6901 actctgaaaa tcgttatgtc cattataata aaagcccgat taagcttatt gcttgggggt
     6961 attttaacgg cattattaac gcagcaacta atttacatac tgttagccct aatttagata
     7021 tcgagaaatt gcgacaattt atcaccgact tgcgcttatc tttcccaatt caagcgccgg
     7081 aagtgaccaa tgatgacatg catcacccta atgaaattcg caatttggtt atagcgctta
     7141 atttagtaca agatccaacc gaacatatcg atgaactacc aaaaaaagtt tatccaagcg
     7201 atctgtttaa ttttggtaag gcacaaaaaa cgttagttgg tagtgtgagc attatttatc
     7261 gaaatgtatg gaatgaaatt cgaactcaac attttgacgg tgaagatgct attttaaaag
     7321 cactaaaatt ggtatccaat cgaatttatc aagaaaattc ttcacctcaa tcagttaatg
     7381 ttttttgcta tagtaaatat ctacgtagtg agttacggga ttttgtggca gatttagttc
     7441 ataaatgtat tacaattcag actggttgtt tactgcaaga gaatgaattt agcactttaa
     7501 aagtagcagg aaaaacctgg cagtttcttt ttcataaaaa aacatttagc attaaacaaa
     7561 ttgatgaaca acaagcggtt gaaaatttaa attcttttgc aaaaaccaag caaaatgcta
     7621 aaagcgaaaa tgaaaagaaa aaagaatttc cgcagcaaat tagtgaattt gccagtgaag
     7681 gctttttaca gtttttcttt gaagacaatg tcgataatag ttttaatgta tatgttgtag
     7741 atgaacgtaa taatgtcgaa agttactata actgtttcgg caataaagaa gagaaaatca
     7801 ggaaaatcaa taaattacaa aatgaaaaac aattaaaaac agaaacacca gcatcatttg
     7861 gaagctttaa tcatccacaa ttttatcaac ttatttcacc aaacggtgag ttatgtattg
     7921 tgccgtttca aagccgccag catttagatt atttagcaca aaaacatcat taatcttatt
     7981 tatattatac aggtgggaat tctatgtcag gattagttag tccggaatta attaaaacag
     8041 tcgtattact tgcaaccagc gtcactattg taccgctatt taaacgcctt ggtttaggaa
     8101 gcgtattggg ttatttagtc gcaggatgtt taatcgggcc atcgggcatt ggattgttcc
     8161 aagaccctgc cgctgttgtg catatggcag aacttggcgt ggttatgttt ttgtttatta
     8221 ttggtttgga aatgcacccg gaactgcttt gggcgatgcg taaagccatc ttcgggcgag
     8281 gtttcttaca agttgcgatt tgcggtactt tattaacctt agccggcatt tacttattag
     8341 gtttttccaa agaagtggca tttattgccg gtatgggttt tacgctatct tctactgcca
     8401 ttgtaatgca agtgctggaa gataaaggga taagttccac gccaaaaggg caacgtattg
     8461 ttgcaacttt actatttgaa gatttagcga ttgtgccatt attagcctcg gttgcctttc
     8521 ttgctcctaa ccaagtagat gctgaatcca gtactaattg gtctgctatt ggtcttagcc
     8581 tagttgccgt tcttattttg gttgcgagtg gaaaatggct gatgaaccct attttccgtg
     8641 ttatttctaa agccaaagta cgagaaatga tgactgccgc tgccttactt gtggtattag
     8701 gctccgctct tttaatggaa atcagtgggc tttcaatggc aatgggggca ttccttgccg
     8761 gagtaatgct ttcggagtct tctttccgcc atcaactaga agctgatatt gaacccttcc
     8821 gtggtttact tttaggctta ttctttatgg gcgtaggaat gtcattagac ctcagcctcg
     8881 tcctcaataa ttggctatgg ttattggtga ttgtagcagt ttatgttatc ggtaaaggcc
     8941 ttggaatctt cattgctgcc tctatcaccc gattaacact gaatgaatct attgtgcgaa
     9001 ctaccattat ggctcacggt ggtgaatttg cctttgtgct attctcagcc gccgcaaccg
     9061 ctgggatttt aaccccagat actcacgcta ccttaaccgc tgcggtaatt gtctcaatgt
     9121 tgctttcccc acttctcatt ttaatcaagc aaggagtgag aaagaaaaaa gcgaaaaatg
     9181 aatccactaa tatgacaggg gttgaattac cggaagattt agaagccaat gtattagtca
     9241 tcggttttgg acgctttaac caaatagtct gccaagcctt attagccaaa ggactaagtg
     9301 tatcggttat tgattcaaat actgatcata ttcgtgctgc tgctcgtttt ggttttaaac
     9361 tttactatgg tgatggagcg agactagatg tacttagggc atctggcatc gccaatgcaa
     9421 actgcgtaat tgttggcgta ggcaatccgg aaagaacgga gaaaatcgta gagctgatta
     9481 aatctgaata ccccttagta cctgtatttg cgagaaccta tgatagacaa agtgcggttg
     9541 atttagtcaa aaagcaccac gttgattatc acattcgaga aacctttgaa tcagccctcg
     9601 cattaagcca agccgccatt gaacgcttag gttctagcga ggaagaggca aaacaaatta
     9661 tcagctatgt tcgccgatta gatgcagaac gctttaatga ggaattatta cacggtttct
     9721 ccgcagaagt tatccgcaaa tattgggtac caacaccttg ggtaaatcca caacacgaag
     9781 gtagtgcact taatgaagaa acggctgata ttttagaaga gcttggtgag acagtagaag
     9841 ttataaaagt ggatatcact gataaaaatt aaagaattta tactttgaat ccttattcaa
     9901 gtataagcct tcctatacat atcgcataat cactcgtaaa aaagcaccct atcggcataa
     9961 tctgataggg tgttaatggt aataaaccat cgcacgatga caaggagaat atctatgaaa
    10021 aaaacaataa atcaatttga acttacattt tttaagaaaa tttggcgtgg agaagtcagc
    10081 cctaattttt ctgcctcgtt aaccaaattc atcgctttat taatatcttt tgctttaatc
    10141 gctttcatta ccgcctgatt aaaataggct tccgtatctt tatctactgc tgtttttact
    10201 gcaactggat tttcttttat cggcaacggt atagccgttt tagctgccgg cgttgctgct
    10261 ttgtttgagc tgaaaatttc agtcggtaat ccaataaatt tagtgccgtt cgagccggta
    10321 acgttgatgt gaatttgccc ttttaaactg tgtgctactt caatatcgtt aattgctggt
    10381 ggttgtttac cggttgcttt tgcataggtt tttgccggat gcggcatcat cgtggttttg
    10441 gctaaatctt gctgcgtggt ataaatgagc aagtaaatat aatcttggtt cattgccggc
    10501 gttaaattaa gttctgcccc taaacggcta cctttgagtc ctcgctcttc caataattta
    10561 aactcagaag aggggtaagt tgatgccaca ttaaattgac tatccaacac gactgccgtg
    10621 ggtacaaaaa catttttgtc catcaccggg ctttcgattt caatttccaa tgtgccttga
    10681 tttgccggaa tacgataagc tgcaaccggg ctggcaatac cggcaaacgc tgtggtaaat
    10741 gcttgtttgt gttgctcgct tagatctgtt tttacggttt gtgaaaatgg cacatcttgc
    10801 cattgaattt gagctaattc agccgagtta atatgaatag acttacctgc cgagccattt
    10861 gctatactcg gaaacaccac caaaagatgg gcgaagaata ttgctgtcga aagaagcgtt
    10921 tttttcatat taacctctaa aaatcgtgaa actaggataa agtgctgaaa tagtgggtag
    10981 gtaatcctac ccacacaaag gtttgatgat taccaccaag cttcaaattg aacaccggtg
    11041 ataaactcgc cgtctttggc tttatagccg gcatcggtac gagctttcgt gttgaattta
    11101 tcattccaac gtgcataagt accaaacaca cggattgcag ggcgagccca aatgctgtta
    11161 cctgcttgcc attgttgtgc aacggtgtat ttcactaagt cgtttttctt gcctgtggct
    11221 tgatctttaa tacggtcata acctacttct aataatgtac tcattgtatc attccatttg
    11281 tacattgggc ggataccggc agaataccaa gttttacctt gtttattatc tagttttgtt
    11341 ttttcgtaga ttaaggcata cattacttca actttatcac tcgcttgcac taccccttgg
    11401 ttgattaaac gaagcatatt gcctttatta tctgcttttg aaccctgtgc gtgaccgtta
    11461 ttccaagagg tcatagaatc ggttgcatat tgtgcggtaa atttattgaa accaccaaag
    11521 aagttacctt gagtgtattc cgctgttacc atataaccgt ttttggttgc tcctttttca
    11581 agggctgcac ctttcttagc atgtgcatta ccgtaatcaa agcctaactc taacgaacca
    11641 tctttccaaa gctcaatacc ggctaaccga acatcaaaaa tatcgttgta aacatcggct
    11701 ttattacgct gcttatctaa tttaatttta cctgtgctag gatctaattt ataaactgta
    11761 gtatatccaa aagtattcgc ccctccacgc tctgtatcac gagtaaccgc taaagagagt
    11821 tttccgaagc ctaaatcaat attttcaacc cctgcacccg gacctgaaat atcccagtaa
    11881 tagaagtcat tcatatgtac atcatggcgt tggtaaaaac gtttaccagc ccataaggtg
    11941 gcacctggta aactatctgc aaagtttttg aactgtacat ttaattcacg tagtgctgga
    12001 cttgttggtg tccaatcatt attgtgaagc gtaccgcc
//
LOCUS       DANKBV010000066        11784 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.066, whole genome shotgun sequence.
ACCESSION   DANKBV010000066 DANKBV010000000
VERSION     DANKBV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.066"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..338
                     /locus_tag="PWY43_001491"
     CDS             <1..338
                     /locus_tag="PWY43_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HDL2136361.1"
                     /translation="VKKALLGYTICVSQATFDEFLEVSARPKFAKYFSIQEREEFIAN
                     LLDAVKFVEIKETITDCQDPKDNKFLEVAISAKAKYLVTGDKKELLSMNPYRNVEIIS
                     VSEFLAETR"
     gene            338..1996
                     /locus_tag="PWY43_001492"
     CDS             338..1996
                     /locus_tag="PWY43_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HDL2136362.1"
                     /translation="MKRFLTLFLLLFSSFSLAETHQIGILAQRGEAYTRTHWQPWVDW
                     LNSQFPNERFELVPLGINEASRRAELDFLLTNQAQFFYLSNQNVRWLATLNSPFSANG
                     EQGAVGSSIWVRADSHYQQLSDLKNQTISAVDNDAFGGFLLGLYQFHQAGMRQNRDFS
                     VQFSGFPVENALALLAEKQVEAAIVPVCLLEELEKEGKFKRSDFRLILQNPQAQGCLA
                     STPLLPNWSLAAMENVPNELAVQLATRLLNSHNPDLPRWTLPFSSAQADHILRELYRH
                     PQQKSLWATVLDWVRLNKFGLLAVVLFILLNLVALRYQVYRKSKALQQAHRKMQQYQQ
                     ELTRADRLALLGEMTTGFAHELKQPLSAVRMYAEGLKSQNSDPYQQRILDKLIAQVDR
                     AVKTMQSIRDWAQNRPSGEPQAVILNQLITNVIEFVAVENRQNAQISLIADRTFRLNL
                     HATVLEQVLTNCLLNALQAGASEISVRLQAVENGLEIAIEDNGGGFSPAQLEFPFVPF
                     RTDKPHGLGLGLVLCQRLMQSLNGRIVLANGENGAKVVLLLPQI"
     gene            2082..2666
                     /locus_tag="PWY43_001493"
     CDS             2082..2666
                     /locus_tag="PWY43_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2136363.1"
                     /translation="MIIHILDDEESILDAMSFLLAPLGVEIQTWQSSLDFLAQADLHR
                     QGVLLLDIRMPLPDGQQVHQQLREAQSTLAVVIMTAHGDVPMAVAELKKGAVDFLQKP
                     ASFEQLKQAIAQAEKVSEKAVKIREISQNYAKLTEKERNLVPLIMQGFTNKQIADKLA
                     ISVRTVEVHRANVMEKMQAESLAGLVQMIGELQY"
     gene            complement(2714..3652)
                     /gene="ttcA"
                     /locus_tag="PWY43_001494"
     CDS             complement(2714..3652)
                     /gene="ttcA"
                     /locus_tag="PWY43_001494"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDL2136364.1"
                     /translation="MTDDIQKSDKKITYNFNKLQKRLRRNVGNAIADFGMIEEGDRVM
                     VCLSGGKDSYTLLDILLNLQQSAPVNFSVVAVNLDQKQPGFPEEVLPRYLESIGVEYK
                     IVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLF
                     LNMFYGGKMKSMPPKLISDDGKQIVIRPLAYCKEKDIIKYAEAKQFPIIPCNLCGSQP
                     NLQRQVVKEMLNTWDRQYPGRLETMFSALQDIVPSHLCDPKLFDFKGIKHGQTIEGIE
                     GDTAFDDVKIEAKTFLDEDDDNNFAEAGMIQFKEVN"
     gene            complement(3715..4428)
                     /gene="ubiG"
                     /locus_tag="PWY43_001495"
     CDS             complement(3715..4428)
                     /gene="ubiG"
                     /locus_tag="PWY43_001495"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HDL2136365.1"
                     /translation="MQNVDQQEITKFENMAKTWWDPNGSFKPIHLLNPLRLAYILEKS
                     NGLFGKKVLDVGCGGGILSEAMAKQGAIVTGIDMTSQPLEVAKQHAKESGLEIDYQQT
                     TIENFLAKMTACNQEKFDVITCMEMLEHVPDPLSIIQSCKALLKPDGVLFFSTINRTF
                     KAYMLVIIGAEYVLKMLPKGTHEFEKFIKPAELLAWCDQADLRCQEMRGYHFNPLTEK
                     FWLNNDVSCNYIATLKPNN"
     gene            4493..4873
                     /locus_tag="PWY43_001496"
     CDS             4493..4873
                     /locus_tag="PWY43_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDL2136366.1"
                     /translation="MTRINLVPPTELCDQHLLAEHRELTRIPNAVAKGKFSLKGQPED
                     YKLGEGHVRFFFNKLTFLKKRYDALHEECLARGFKVQYFWAEHLPQDPSLWLDYQPTE
                     TALTVNRERIALRMPANPRFSERK"
     gene            4970..7666
                     /gene="gyrA"
                     /locus_tag="PWY43_001497"
     CDS             4970..7666
                     /gene="gyrA"
                     /locus_tag="PWY43_001497"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDL2136367.1"
                     /translation="MSELAKDIIPVSIEDELKTSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFSMDQNSNTYNKPHVKSARVVGDVIGKYHPHGDFAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDAPAAMRYTEVRMQKITQELLTDLDKETVDFSPNYDGKEMIPDVLP
                     TKIPALLVNGSSGIAVGMATNIPPHNLGEVLDGCLAYIENEQISVEELMRYIPGPDFP
                     TGAIINGRKGIEEAYRTGRGKVYVRAKASVETTDKGREQIVVTELPYQVNKAKLIEKI
                     AELVKEKKIEGISKIDDYSDKKGISIVIEVKRDAVGEVVLNHLYSLTQMQVTFGINMV
                     ALDNGQPKVLNLRQIIEAFVKHRREVVTRRTIFELRKARERAHILEGLAIALANIDPV
                     IELIRASKTAEEAREGLLARPWALGNVAPMLEAAGVGASRPEDLPEHLGVRDGEYYLS
                     EAQARAILELRLHRLTGLEHEKIVEEYKELLTEIGELIHILTSPERLMEVIREELERV
                     KTDFNDERRTEITAASGDINIEDLIAQEDVVVTLSHAGYVKYQPLSDYEAQRRGGKGK
                     SATKMKEDDFIEKLLVANTHDTILCFSSRGRLYQLKVYQLPQASRGARGTPIVNILPL
                     VKEENERITAILPIPNGEFSADKFIFMATASGVVKKVSLDAFSNVRASGLIALKLREG
                     DELIGVDITDGNSEIMLFSAQGRVVRFAETAVRAMGRTATGVRGIKLSTTEISSEEIE
                     EAEIIETDNEEAEDSSSVSLDKDRVVSLVIPRTEGDILTVTQNGYGKRTVISEYPVKS
                     RATKGVVSIKVNERNGKVVAAVQVEETDQIMLITDAGTLVRTRVAEVSLVGRNTQGVR
                     IIRTAEDEQVVSLERVCEPEDDENESLDALENAETVETSTEE"
     gene            7762..9309
                     /locus_tag="PWY43_001498"
     CDS             7762..9309
                     /locus_tag="PWY43_001498"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL2136368.1"
                     /translation="MAAIQQRAELHRQIWQIANEVRGAVDGWDFKQYVLGSLFYRFIS
                     ENFSAYIEQGDESIDYAAYSDEEIDAFGIKEDAIKSKGYFIYPSQLFKNVVKNANTNN
                     NLNIELADIFADIEASAVGFDSEKDIKGLFADFDTKSNRLGNTVEDKNKRLAAVLKGV
                     ESLNFGDFAENQIDLFGDAYEYLISNYASNAGKSGGEFFTPQNVSKLIAQLALYGQSA
                     VNKIYDPACGSGSLLLQAKKQFDDHLIEEGFFGQEINHTTYNLARMNMFLHNINYDKF
                     HIELGDTLINPKLKDDKPFDAIVSNPPYSIKWIGSDDPTLINDERFAPAGILAPKSKA
                     DFAFILHALNYLSAKGRAAIVTFPGIFYRSGAEQKIRQYLIEQNVVESVIALSANLFY
                     GTSIATNILVLSKHKTDTKTQFIDASDLFKKETNNNVLTDEHIAQILKLFADKADVEH
                     FAKSVDNRQIAENEYNLAVSSYVEAKDTREVINITELNKEIRKTVAKIDRLRADIDAI
                     VAEIEQQ"
     gene            9385..10596
                     /locus_tag="PWY43_001499"
     CDS             9385..10596
                     /locus_tag="PWY43_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2136369.1"
                     /translation="MSNSIIEKLLNGAEVEWKPLGEVAELKRGKTITAKDKTEGNIPV
                     ISGGQKPAYYTGEYNREGETITIAGSGAYAGFLMYWNEPIFLGDAFSVKPDLDILITK
                     YVYHFLLSKQQWIFNLKKGSGVPHVYPKDLAILEIPIPPLEIQQKIVKTLDKFTELEA
                     TLEATLEAELALRKKQYQYYRETLLTFPQDLDRGGYNDITKAIGQVVGKNAHPTVVWK
                     SLGEIGEARMCKRILKEQTSNVGDIPFYKIGTFGKKPNAYISRELFEDYKQKYSYPRK
                     GNILISASGTIGRTVIFDGEDSYFQDSNIVWIENDESQVLDKFLFYLYQIADWNIAEG
                     GTIQRLYNDNLKKLKIPVPPLSEQQKIVNILDKFDSLTNSITEGLPKEIKLRREQYGY
                     YREQLLNFPKP"
     gene            10606..>11784
                     /locus_tag="PWY43_001500"
     CDS             10606..>11784
                     /locus_tag="PWY43_001500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anticodon nuclease"
                     /protein_id="HDL2136370.1"
                     /translation="MSKILDDIALELRNNKKKVQLIYAFNGTGKTRISRKFKELISPK
                     LEDDTEYSGELKHKKILYYSSFTEDLFYWDNDLEYDSKIKLKIHKNSFTDWVFIEQGL
                     EDTVTRIFQHYTNEKLTPNFNEEYTFQNEYGDNITIPAYSEIVFSYARGDDAFQENIK
                     ISKGEESNFVWSVFYSLFEQVISELNIAEIKNRSTDKFNNLEYVFIDDPVSSLDENHF
                     IELAIDIAQLIQSSESNLKFIITTHNPLFYNVLHNEFRRENSISYRLEKINYDDFILS
                     KQESDSPFAYQVYLKNQLEDLFKEEVLAEKLKELIKKVISKDKEEKIERLFTNGLTQE
                     ELEEFHKEFPEFNDLYVIKTYFKKENKKVFYKVKFFKDKEIDDCKVEKYHFNFIRNIL
                     E"
BASE COUNT         3603 a         2270 c         2666 g         3245 t
ORIGIN      
        1 cagtgaaaaa agccttatta ggctatacca tttgtgtctc gcaagcgact tttgacgaat
       61 ttttagaagt ctccgctaga ccgaaatttg ctaaatattt cagtattcag gaaagggaag
      121 aatttattgc caatttgttg gatgcagtaa agtttgtgga aattaaagaa acgattacag
      181 attgccaaga tcccaaagat aataaatttt tagaagtggc gatcagtgca aaagcgaagt
      241 atttagtgac gggcgataaa aaagagctcc tcagtatgaa cccttatcgg aatgttgaaa
      301 ttatttctgt cagcgaattt ttagcggaaa ctcgctaatg aaacggtttc tcactctttt
      361 tcttctcctt ttttcatctt tttcattagc cgaaacccac caaatcggca ttctcgctca
      421 gcgtggggaa gcctacaccc gcacccattg gcagccttgg gtggattggt taaattcgca
      481 gtttcccaac gagcggtttg agctggttcc attggggatt aacgaggcga gccgccgggc
      541 ggagttggat tttctgctga ccaatcaagc tcagtttttc tatttgagta accaaaatgt
      601 gcgatggctt gccacgctca acagtccgtt ttcggcaaat ggtgagcaag gggcggtggg
      661 cagtagcatt tgggtgcgtg ccgacagcca ttatcagcag ttaagcgatc tgaaaaatca
      721 gactatcagt gcggtggata acgatgcttt cggcggtttt ttgcttgggt tgtatcagtt
      781 tcatcaagca ggaatgcggc aaaatcggga tttttcggtg cagtttagcg gttttccggt
      841 ggaaaatgcg ttggctttgc tggcggaaaa acaggtggaa gcggcgattg taccggtctg
      901 tttgctggaa gagttggaga aagagggcaa gttcaagcgg tcggattttc gcttaatttt
      961 gcaaaaccca caggcgcagg gttgccttgc cagcacgccg ttgttgccga actggtcgct
     1021 ggcggcaatg gaaaatgtgc caaacgagct ggcggtacag cttgccacac gcttgctaaa
     1081 cagccacaat cccgacctac cccgctggac gttgccgttt tcttccgccc aagccgatca
     1141 cattctgcgt gaactgtatc gccacccgca acaaaaaagc ctgtgggcaa cggtgctgga
     1201 ttgggttcgt ctcaataaat tcggcttgtt ggcggtggtg ttgttcattt tgctcaacct
     1261 tgtcgccctg cgttatcagg tctatcgcaa aagcaaggcg ttacagcagg cacaccgcaa
     1321 aatgcagcag tatcagcagg aactgacccg agccgaccgc cttgctttgt tgggcgagat
     1381 gacaaccggt tttgctcacg aactgaaaca accgttgtcg gcggtgcgaa tgtacgccga
     1441 ggggctgaaa agccaaaaca gcgatcctta tcaacagcgg attttggata agctgattgc
     1501 gcaggtagat cgggcagtga aaacgatgca gagcattcgg gactgggcgc aaaaccgccc
     1561 gagtggtgaa ccgcaagcgg tcattttgaa ccaattgatt actaatgtga tcgagtttgt
     1621 ggcggtggaa aatcgccaga atgcacaaat ctcgctgatt gccgaccgca cttttcggct
     1681 taatttacac gccacggtac tggaacaagt actgacgaac tgcctgctca acgccctgca
     1741 agccggtgca agcgagatat cggtgcggtt acaagcggtc gaaaacgggc tggaaattgc
     1801 aatcgaagac aacggcggcg gtttttcccc tgcccagctt gaatttccgt ttgtgccatt
     1861 tcgcaccgac aaaccacacg gcttagggct aggcttggtg ctatgccaac ggctgatgca
     1921 atcgctcaac ggacggattg tattggcgaa cggggagaac ggggcgaagg tagtgctttt
     1981 actccctcaa atataaatgt aggggcaggt cctgtgcctg cccgtaaatc ctatcaaatt
     2041 cagggcgggc acaggacccg cccctacgga aaaaagaaaa aatgattatt cacattcttg
     2101 atgacgaaga aagcatttta gacgctatgt cgttcctgct tgccccgctt ggggtggaaa
     2161 tccaaacgtg gcaatccagc ctagattttt tggcacaggc ggatttacac cggcaaggcg
     2221 tgctgttact agatattcga atgccgctgc cagacgggca acaggttcat caacaactgc
     2281 gagaggcaca atcgaccctg gcggtggtga ttatgaccgc ccacggtgat gtgccgatgg
     2341 cagtcgctga attgaaaaaa ggcgcagtgg attttctgca aaagccggca agttttgaac
     2401 agctaaaaca ggcgattgca caggcggaaa aagtaagtga aaaagcggtg aaaattcgtg
     2461 aaatttcgca aaattacgcc aaattgacgg aaaaagagcg taaccttgtg ccactgatta
     2521 tgcaaggttt caccaataaa cagatcgcag ataaattagc gatttcagtg cgtacggtgg
     2581 aagtacatcg agccaatgtg atggaaaaaa tgcaggcgga gagtttggcg gggctggtgc
     2641 agatgatcgg ggagttacaa tattaaaggt agcggaaatt gctctgctac ctttagtttc
     2701 gacttttgcc gtattaattt acttctttaa actgaatcat tcccgcttcg gcaaaattgt
     2761 tatcgtcatc ttcgtctaag aaggttttgg cttcaatttt gacatcatca aaagcggtgt
     2821 cgccttctat gccttcaatg gtttgaccgt gtttaatccc tttaaaatca aacaatttcg
     2881 gatcgcataa gtgggatggc acgatatctt gcaaagcact gaacattgtt tctaaacgcc
     2941 ccgggtattg gcgatcccaa gtattcagca tttctttcac cacttggcgt tgtaaattag
     3001 gctgagaacc acacagatta cacggaataa tcgggaactg cttggcttcg gcatatttga
     3061 taatgtcttt ttctttacaa taggcgagtg ggcggatcac aatttgcttg ccgtcatcag
     3121 agatcagttt aggtggcata cttttcattt tgccgccgta gaacatattt aagaataaag
     3181 tcgccagcat atcgtcacgg tgatgcccta atgcaatttt ggttgcaccc aattcggtgg
     3241 cggtgcggta taaaatccca cggcgaagtc gtgagcaaag tgaacaggtg gttttgccct
     3301 ccggaatttt ctcttttacg atgccgtagg tgttttcttc cacaattttg tattccacgc
     3361 caatgctttc taaatagcga ggcaatacct cttccggaaa tcccggttgc ttttggtcta
     3421 aattgaccgc taccacacta aaattcaccg gtgcactttg ctgtaaattg agcaaaatat
     3481 ctaacagcgt atagctgtct ttaccgccgg aaaggcagac cattacacgg tcgccctctt
     3541 caatcatacc aaaatcggca atggcattac cgacattacg gcgtaaacgt ttttgtaact
     3601 tgttgaaatt ataagtaatt tttttatcag atttttgaat atcgtctgtc ataatagttg
     3661 tactaggctt agaaattagc ggtgtatagt aaagatttcg cactattttg caagttaatt
     3721 attcggcttc agcgtagcga tatagttaca actcacatca ttattgagcc aaaatttttc
     3781 ggttaatggg ttgaaatggt agcctctcat ctcttggcaa cgtaaatcag cctgatcgca
     3841 ccaagctaac aattcggcag gcttgatgaa tttttcaaat tcgtgcgtgc cttttggcaa
     3901 cattttcagc acatattccg ccccgataat tactaacata taggctttaa aagtacggtt
     3961 gatggtagag aaaaacagca cgccgtcagg tttgagcagg gctttacagc tttgaatgat
     4021 agaaagcgga tcgggaacgt gttccagcat ttccatacaa gtaatcacat cgaatttttc
     4081 ttgattacaa gcggtcattt ttgctaaaaa attttcaatc gtggtttgtt ggtaatcaat
     4141 ctctaaccca ctttctttgg cgtgctgctt tgccacttca agcggttgag aggtcatatc
     4201 gatgccggta acaatcgctc cctgttttgc cattgcctca cttaaaatac cgccaccaca
     4261 gccgacatct aacacctttt tgccaaataa accgttcgat ttttctaaaa tataggctaa
     4321 acgcagtgga ttaagcagat gaatcggctt gaaactgccg tttggatccc accaggtttt
     4381 agccatattt tcaaattttg tgatttcttg ctgatcgacg ttttgcataa actgttccta
     4441 tggtaaattt agggcattac tataacgcat ttagcctaaa aggaaaatca gaatgacccg
     4501 aattaacctt gttccgccaa cggaattatg cgatcaacat ctgcttgccg aacatcgtga
     4561 acttactcgg attccgaatg cggtagcgaa aggcaaattt agcctaaaag gacagccaga
     4621 agattacaaa ctgggcgagg gacacgttcg gtttttcttc aataaactca cgtttttgaa
     4681 aaagcgttac gatgcgttac acgaggaatg tttagcaaga ggttttaagg tgcaatattt
     4741 ctgggcggaa catttaccac aagatccctc tctctggcta gattatcagc cgactgaaac
     4801 cgctctgact gtaaatcggg agcgaattgc attaagaatg ccggcaaatc cacgttttag
     4861 cgaaagaaaa tgaccgcttg tacgcctttt tgttgctttt tatgctataa tctcgccaat
     4921 tttttcgcct tatttaaggc tcttttacta ataaaattag gaaatttcaa tgagcgaatt
     4981 agccaaagat attattcccg taagtattga ggacgaatta aaaacctctt accttgacta
     5041 tgcgatgtcg gtgattgttg ggcgtgcctt gcctgatgta cgagacggtt taaagccggt
     5101 acaccgccga gtgctttttt caatggatca aaacagcaac acctataata agccgcacgt
     5161 taaatctgcc cgtgtggtcg gtgatgtgat cggtaaatat cacccacacg gtgactttgc
     5221 ggtgtatgac actattgtgc gtatggctca accgttctca ctacgttata tgttggtgga
     5281 cgggcaaggt aactttggtt ctattgatgg cgatgcaccg gcagcaatgc gttataccga
     5341 agtgcgtatg cagaaaatta cgcaggaatt attaaccgat ctcgataaag aaacggtgga
     5401 tttctcaccg aactatgacg gcaaagagat gattcctgat gtattgccaa ccaaaattcc
     5461 ggctctgtta gtcaatggtt cttcagggat tgcggtcggt atggcaacca atattccacc
     5521 tcataattta ggcgaagtat tagatggttg cttggcgtat attgaaaatg aacagattag
     5581 tgttgaagag ctaatgcgtt atatcccggg gccggatttc ccgaccgggg cgattatcaa
     5641 cggtcgtaaa gggattgaag aggcttaccg caccggtcgt ggcaaggtgt atgtgcgcgc
     5701 gaaagccagc gtggaaacaa ctgataaagg gcgtgagcaa attgttgtaa ccgaactgcc
     5761 ataccaagtg aataaggcaa aattaattga aaaaattgcc gagctggtta aagagaagaa
     5821 aatcgaaggc atcagcaaaa ttgatgacta ttccgataaa aaagggattt cgattgttat
     5881 tgaagtcaag cgtgatgcag tgggtgaagt ggtgttaaac cacctttatt ctttaaccca
     5941 aatgcaggtc acgtttggta tcaatatggt ggcgttagat aatgggcagc caaaagtgct
     6001 gaacttacgc cagatcatcg aagcctttgt taaacaccgt cgtgaagtgg tcacacgccg
     6061 tacgattttt gaattgcgta aagcccgtga acgtgcccat attttagaag gtttggcgat
     6121 tgcgttagcg aatatcgacc cggtaattga attaattcga gcctcaaaaa ccgcagaaga
     6181 agcccgtgaa ggcttattgg ctcgcccttg ggcattaggc aatgttgcac caatgttaga
     6241 ggctgctggt gttggtgctt cccgaccgga agatttacct gaacacttag gagtgcgtga
     6301 tggtgaatac tatttatctg aagctcaagc cagagcgatt ttagagctac gcttacaccg
     6361 cttaaccggt ttagaacacg agaaaatcgt tgaagaatac aaagaactct taactgaaat
     6421 tggcgaatta atccacattt taaccagccc agaacgctta atggaagtga tccgtgaaga
     6481 gttagaacgt gtaaaaaccg attttaatga tgaacgccgt actgaaatta ccgcagcctc
     6541 tggcgacatc aacattgaag acttaatcgc acaagaagat gtggtggtta cgctttctca
     6601 cgccggctat gtgaaatatc agccgttatc cgactacgaa gcacaacgcc gtggcggtaa
     6661 aggtaagtcg gcaaccaaga tgaaagaaga tgatttcatc gaaaaactct tggtggcaaa
     6721 cacccacgac accattcttt gcttctcaag ccgtgggcgt ttatatcaat taaaagtgta
     6781 tcaattaccg caagcaagcc gtggggcaag aggcacgccg attgtgaata ttctgccgtt
     6841 agtgaaagaa gaaaacgaac gtattaccgc gattctaccg attccaaacg gtgaatttag
     6901 tgccgataag tttatcttta tggcaacagc tagcggtgta gtgaaaaaag tttcccttga
     6961 tgcctttagc aacgtgcgtg caagcggtct gattgcgtta aaacttcgtg aaggcgatga
     7021 gctgatcggg gtagatatta ctgatggtaa cagcgaaatt atgttgttct cggcacaagg
     7081 gcgagtagtt cgctttgccg aaacagcagt tcgtgcgatg ggacgtaccg ccaccggcgt
     7141 acgaggtatt aaactttcta ccaccgaaat ttcaagcgaa gaaattgaag aagctgaaat
     7201 tattgaaact gacaatgaag aggcagaaga ttcctccagc gtcagccttg ataaagaccg
     7261 tgtggtttct ttagtgattc ctcgcaccga aggtgatatt ttaaccgtca cccaaaacgg
     7321 ctacggtaaa cgtacggtga ttagcgaata tccggtgaaa tcccgagcaa ccaaaggggt
     7381 cgtttcgatt aaagtgaatg aacgaaacgg caaagtggtt gcggcagttc aagtagagga
     7441 aaccgaccaa attatgttga ttaccgatgc cggcacgctg gttcgcaccc gagtggcaga
     7501 agtcagcctc gttggacgaa acacccaagg cgtacgtatt attcgcaccg cagaagacga
     7561 acaagttgtt agccttgaac gagtctgcga gcctgaagac gatgaaaatg aaagcctaga
     7621 cgcattagaa aatgccgaaa cagtagaaac atcaactgaa gaataatctt aactaaacaa
     7681 gcggtcgaat tttcacaatt ttttgcaaaa atagaaggaa attcgaccgc tttaatatat
     7741 gagcaatatt aggaatacaa aatggcagct attcaacaaa gagcagaact ccatcgtcaa
     7801 atttggcaaa ttgcaaacga agtgcgtggt gcggtggacg gctgggattt taaacaatat
     7861 gtattaggtt cgctgtttta tcgttttatt agcgaaaatt tttccgccta tatcgaacaa
     7921 ggcgatgaaa gtattgatta cgcagcatat tccgacgaag agattgatgc attcggtatt
     7981 aaagaagatg cgattaagag caaaggctac tttatttacc caagccagtt gtttaaaaat
     8041 gtcgtgaaaa atgccaatac caataacaat ttgaatattg aactcgccga tatttttgcc
     8101 gacattgaag cttctgcggt agggtttgat tcggaaaagg atattaaagg cttgtttgcc
     8161 gattttgata ccaaaagtaa ccgcttgggt aataccgttg aagataaaaa caagcgatta
     8221 gcggcggtat tgaaaggggt agagtcgctt aattttggtg attttgccga gaaccagatc
     8281 gacctgttcg gcgatgccta tgaatatcta atttctaatt atgcgtctaa cgcaggtaaa
     8341 tcgggcggtg aatttttcac gccacaaaat gtgtctaaac tcattgcaca acttgcttta
     8401 tacgggcaaa gtgcggtcaa taaaatctat gatccggctt gtggttccgg ctcactgtta
     8461 ttgcaagcga aaaagcagtt tgacgatcat ttgattgaag aaggcttttt cgggcaggaa
     8521 atcaaccaca cgacttacaa cctcgcccga atgaatatgt tcttgcataa tattaactat
     8581 gacaaattcc atattgagct gggcgacaca ctgattaatc caaaactcaa agatgacaaa
     8641 ccttttgatg cgattgtctc taacccacct tattcaatta aatggattgg ctcggacgac
     8701 cctacactca tcaacgatga gcgttttgct cctgccggca ttcttgctcc gaaatccaaa
     8761 gccgattttg cctttattct gcacgccctg aactaccttt cagcaaaagg acgggcggcg
     8821 attgttactt tccccggcat tttctaccgt agtggtgcgg agcaaaaaat ccgtcagtat
     8881 ttaattgaac agaatgtggt agaaagtgtg atcgccttgt cagcaaatct cttttacggc
     8941 accagcattg cgactaatat tttagtgcta tctaaacaca aaactgacac caaaacccaa
     9001 tttattgatg caagcgatct gtttaagaaa gaaactaaca ataatgtgct gaccgatgaa
     9061 catatcgctc aaattctcaa attatttgcc gataaagccg atgtggagca tttcgccaaa
     9121 tcggtagata accgccaaat tgcggagaat gagtacaatc ttgcggttag ctcttatgtg
     9181 gaagcaaaag atacccgaga agtgatcaat atcaccgaac tcaataaaga aattcgcaaa
     9241 acagtcgcaa aaattgaccg cttgcgggca gatattgatg cgattgtggc ggagattgaa
     9301 caacagtgat cgtggataat aaaaagggcg gatcacattc gcccaaataa aacaagcggt
     9361 ataaaatcaa ggaaaatttg caaaatgagt aatagtatta ttgagaaatt attaaatggg
     9421 gctgaggtcg agtggaagcc gttaggagag gtcgcagagt taaaacgcgg aaaaacaatt
     9481 acagcgaaag ataaaacaga agggaacatt cctgtaatta gtggtggtca gaagccagca
     9541 tactatactg gcgaatataa tagagagggt gaaacaatta ctattgcagg aagtggggca
     9601 tatgctggtt tcttaatgta ttggaatgaa ccaatttttc ttggagatgc tttttccgta
     9661 aaaccagatt tagatattct tatcacaaaa tatgtatatc attttttgtt atctaaacag
     9721 cagtggattt ttaatttaaa aaaagggtca ggtgttccac atgtttatcc aaaagattta
     9781 gctatattgg aaatccccat tcctccactc gaaattcaac aaaaaattgt aaaaacactt
     9841 gacaaattca cggagctgga agctacgctg gaagctacgc tggaagctga attagctctg
     9901 cggaaaaaac aatatcaata ttaccgagaa accctattga cattcccaca agatttagat
     9961 aggggggggt ataacgacat caccaaagcc atcgggcaag tggtgggcaa gaatgcccac
    10021 cctacggtgg tttggaagag tttgggggag atcggtgaag ctagaatgtg taaaagaatt
    10081 ttgaaagagc aaactagcaa tgttggagat attccgtttt acaaaattgg aacgtttgga
    10141 aaaaaaccga atgcttatat ttcaagagaa ttatttgaag attacaaaca gaaatatagt
    10201 taccctagaa aagggaatat cttaatttct gcatctggaa caatagggag aacagtaatt
    10261 tttgatggcg aagattctta ttttcaagat agtaatattg tttggattga aaatgatgaa
    10321 agtcaggtct tagataagtt tttattttat ctttatcaaa ttgctgattg gaatatagca
    10381 gaaggcggaa caattcagag gttgtataat gataatttaa aaaaattaaa aattcctgtt
    10441 cctcctcttt ccgaacaaca aaaaatcgtc aatattttag ataaattcga cagcttaacc
    10501 aattcaatca ccgagggttt accaaaggaa attaaattaa ggcgagaaca gtatgggtat
    10561 tatcgtgagc agttattaaa ttttcctaag ccataaggat aacatatgag taaaatttta
    10621 gatgatattg ctctagaact aagaaataat aagaaaaaag tacagctcat ttatgctttt
    10681 aatgggactg ggaaaacaag gatatctcga aagtttaaag agcttatttc tcctaaatta
    10741 gaggatgata ctgagtatag tggagaatta aagcataaga aaattctata ctattcctct
    10801 tttacggaag atttatttta ttgggataat gatttagagt atgattctaa aattaaatta
    10861 aaaatacata aaaactcctt cactgattgg gtttttatag agcaggggct agaagataca
    10921 gtaactagga tttttcagca ttatactaat gaaaaactaa cccctaattt taacgaagaa
    10981 tatactttcc aaaatgagta tggagataat attactatcc cagcctattc agaaatagtt
    11041 ttctcctacg ctagaggcga tgatgctttt caggaaaata ttaaaatttc taaaggggag
    11101 gaaagtaatt ttgtttggag tgttttttat tcgctgtttg agcaagttat atcagagtta
    11161 aatattgctg aaataaaaaa tcgttcaaca gataaattta ataatttaga atatgttttt
    11221 attgatgatc cagttagctc actagatgaa aatcatttta ttgaattggc aattgatatt
    11281 gcccaattaa tacaatcaag cgaatcaaat cttaaattta ttattacaac acacaatcca
    11341 cttttttata atgtgttaca taatgaattc agaagggaaa atagtatttc ttataggtta
    11401 gaaaagataa attatgatga ctttattctt tctaaacaag aaagtgattc tccatttgca
    11461 tatcaggtat atctcaaaaa ccaattggaa gatctattta aagaagaagt tttagcagaa
    11521 aaattaaaag aattgattaa gaaggtcatc tcaaaagata aagaagaaaa aatagaaaga
    11581 ttatttacta atggattaac acaagaagaa ttagaagagt ttcataaaga atttccagag
    11641 tttaatgatt tatatgtaat taaaacatat tttaagaaag aaaataaaaa agttttttat
    11701 aaggtgaaat tttttaagga caaagagata gatgattgta aagtggaaaa atatcacttt
    11761 aattttatta ggaatatttt ggaa
//
LOCUS       DANKBV010000067        11730 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.067, whole genome shotgun sequence.
ACCESSION   DANKBV010000067 DANKBV010000000
VERSION     DANKBV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11730
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.067"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..721)
                     /gene="mutY"
                     /locus_tag="PWY43_001501"
     CDS             complement(<1..721)
                     /gene="mutY"
                     /locus_tag="PWY43_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL2136371.1"
                     /translation="MNSNSPFPLAKAKASDPFAKAVLAWFEQYGRKHLPWQQNKTLYQ
                     VWLSEVMLQQTQVATVIPYFARFMERFPTVIDLANASIDEVLHLWTGLGYYARARNLH
                     KAAIQIRDEFGGEFPTRFDDVLALTGVGRSTAGAILSSVLDAPHPILDGNVKRVLSRY
                     FAVEGWAGEKAVENRLWQLSESVTPDTQVADFNQAMMDLGAMICTRTKPKCLLCPLQE
                     NCKTNAMQAWDSFPAKKPKKTL"
     gene            805..1197
                     /locus_tag="PWY43_001502"
     CDS             805..1197
                     /locus_tag="PWY43_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2136372.1"
                     /translation="MKSPILGFHHIALIVSDYEKSKHFYTRILGAEIIAETYREQRAS
                     YKLDLRFKDGSQIELFSFPETPPRFTSPETCGLRHLAFRVENIEYAIAFLEQHQLPHE
                     GIRIDEITGKRFTFFRDPDDLPLEFYEV"
     gene            1198..2067
                     /locus_tag="PWY43_001503"
     CDS             1198..2067
                     /locus_tag="PWY43_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL2136373.1"
                     /translation="MLYLLIATLIWASSFIVGKVAISAFDPVQIMQIRLTLAAFIALP
                     FFIPAYKKIQKNQHIKVWLISFLTFPLCVILQFSGLKLTSAASAVALLGLNPLLTVLV
                     SYFCFKKQATRLDFILSLFACLGILIMASGSDDSGSINVLGLLLVTLGSLSFIVGMYL
                     SKEILQSVKVIDLTKITLVQGAITALPITLLFAENWQLPTSLSAWASVAYLGIICTSL
                     AILLWNKGISQSSPILAGILLALEPVFGLLMALVFLSEQLSLVTWVGIIIVICTALLS
                     VLLPMMKRYKRLI"
     gene            2083..2796
                     /gene="sfsA"
                     /locus_tag="PWY43_001504"
     CDS             2083..2796
                     /gene="sfsA"
                     /locus_tag="PWY43_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL2136374.1"
                     /translation="MKFPPLSHGILLRRYKRFLADIQLPNGEQITLHCPNTGAMTGCA
                     NPGDTVWFSTSHNPKRKYAHTWELTQTQAGDFICVNTLRANQLVQEALENQWISELAD
                     YQHILPEQKYGSENSRIDFLLKSDLLPDCFVEVKSTTLLTENGVGMFPDAKTERGQKH
                     LRELTKIAEQGLQAVIFFAILHTGIERFEVAKQIDPKYAELFAQAIAQKVQVLSYKMA
                     ISLENGQPIATTLTKKCEF"
     gene            2857..3378
                     /gene="hslV"
                     /locus_tag="PWY43_001505"
     CDS             2857..3378
                     /gene="hslV"
                     /locus_tag="PWY43_001505"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDL2136375.1"
                     /translation="MTTIVCVRKDGKVAIGGDGQATLGNCIEKGTVRKVRRLYKDKVV
                     TGFAGSTADAFILRDLFEKKLELHQGHLVKSAVELAKEWRTERALRRLEAMMIVANDS
                     EFLLVSGSGDVIEPEQDVLAIGSGGNYAKAAALALLRTENNLSAKEIVAEALKIAGDI
                     DIYSNHKHVIEEV"
     gene            3430..4755
                     /gene="hslU"
                     /locus_tag="PWY43_001506"
     CDS             3430..4755
                     /gene="hslU"
                     /locus_tag="PWY43_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDL2136376.1"
                     /translation="MMSMTPREIVSELDAHIIGQKDAKRAVAIALRNRWRRMQLPEDL
                     RQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LADVSMKLVRQQAVEKNKMRAQDAAEDRILDVLLPPAKDQWGNVQESDNNSTRQTFRK
                     KLREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPSKTKKRKMKIKD
                     ALKVMLDEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQ
                     RDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQGRLPIRVELKSLT
                     KEDFERILTEPNASLTLQYRELMKTEGVEIEFTKGGISRIAESAFRVNEKTENIGARR
                     LHTVLERLMDGISFDASERRGEKIIIDENYVSEALNDVVENEDLSRFIL"
     gene            4831..5709
                     /locus_tag="PWY43_001507"
     CDS             4831..5709
                     /locus_tag="PWY43_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDL2136377.1"
                     /translation="MKNLYKWPAAQSLYPNQGKKSYAGKRFRYRLRSWLHHSKIKQFE
                     QFVTQNPQLIPLLNARPNYSYPVAHRFLDKRFSAKQRLQKITDNLLFLPQKLAHLPPL
                     WEQAVNFGEIIADFELWLNINEHQPMEGFWALELRHRPTNQLVYLLTFGKLDEALLIA
                     VIQGPNFEDSKELVKQLTKSCHGLRPAYLMVETMKALTAVLGYQSLLGIPQKYQNKSR
                     WVQSKRYVVDYDAIFAESGGKPGNYWQLPLELESKKIDEIPSKKRSMYRKRYEMLANL
                     KQTMELMLKTYTGLNG"
     gene            complement(5769..6485)
                     /locus_tag="PWY43_001508"
     CDS             complement(5769..6485)
                     /locus_tag="PWY43_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2136378.1"
                     /translation="MKKTLLTLLFGCVVTAQAQDIKFVMEPSYPPFEMTDEKGEIIGF
                     DVDIANAICKEMNANCTFHSQPFDSLIQSLKQKQFDAAISGMGIAEPRKKQVLFSEPY
                     FPSSAAFIAKKDIDFAKVKTIGVQNGTTYQHYLAKEKKEYNVKSYASYQNAILDVQNG
                     RIDAIFGDVPVLAEMARKHEGLDFVGEKINNPNYFGDGLGIATHLKNQVLVDQFNAAL
                     KTIKENGEYQKIYDKWMGGK"
     gene            6882..7385
                     /locus_tag="PWY43_001509"
     CDS             6882..7385
                     /locus_tag="PWY43_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HDL2136379.1"
                     /translation="MNQSYFNLLGNITWLWMNSSLHKEWSCELLARNVIPAIENEQYM
                     LLIDNGIPIAYCSWADLNLETEVKYIKDISSLTPEEWQSGDRRWIIDWVAPFGHSQLL
                     YKKMCQKYADTLVRSIRFQPNQKSVGKIAYFKGGKLDKKTAKKRFDTYQEELATALKN
                     EFNFIKK"
     gene            7401..10262
                     /locus_tag="PWY43_001510"
     CDS             7401..10262
                     /locus_tag="PWY43_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX family hemolysin"
                     /protein_id="HDL2136380.1"
                     /translation="MGNKLTNISTNLKSSWLTAKSGLNRTGQSLAKAGQSLKTGAKKI
                     ILYIPKDYQYDTDKGNGLQDLVKAAEELGIEVQKEESNDIAKAQTSLGTIHNVLGLTE
                     RGIVLSAPQLDKLLQKTKVGQAIGSTENITKGFSNAKTVLSGIQSILGSVLAGMDLDE
                     ALQNNSNELTLAKAGLELTNSLIENIANSVKTLDAFGDQINQLGSKLQNVKGLSSLGE
                     KLKGLSGFDKTSLGLDIVSGLLSGATAALVLADKNASTSRKVGAGFELANQVVGNITK
                     AVSSYILAQRVAAGLSSTGPVAALIASTVSLAISPLSFAGIADKFNHAKSLESYAERF
                     KKLGYDGDNLLAEYQRGTGTIDASVTAINTALAAIAGGVSAAAAGSVVASPIALLVSG
                     ITGVISTILQYSKQAMFEHVANKIHNKIVEWEKNNPGKNYFENGYDARYLANLQDNMK
                     FLLNLNKELQAERVIAITQQQWDNNIGDLAGISRLGEKVLSGKAYVDAFEEGQHLKAD
                     KLVQLDSAKGIIDVSNTGEAKTQHILFRTPLLTPGTEKRERVQTGKYEYITKLHINRV
                     DSWKITDGAASSTFDLTNVVQRIGIELDNAGNVTKTKETKIIAKLGEGDDNVFVGSGT
                     TEIDGGEGYDRVHYSRGNYGALTIDATKETEQGSYTVNRFVESGKALHEVTSTHTALV
                     GNREEKIEYRHSNNQHHAGYYTKDTLKAVEEIIGTSHNDIFKGSKFNDAFNGGDGVDT
                     IDGNDGNDRLFGGKGDDIIDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRQGDGNDSIT
                     ESEGNDKLSFSDSNLKDLTFEKVNHHLVITNTKQEKVTIQNWFREAEFAKTIQNYVAT
                     RDDKIEEIIGQNGERITSKQVDELIEKGNGKIAQSELTKVVDNYQLLKYSRDASNSLD
                     KLISSASAFTSSNDSRNVLASPTSMLDPSLSSIQFARAA"
     gene            10337..>11730
                     /locus_tag="PWY43_001511"
     CDS             10337..>11730
                     /locus_tag="PWY43_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_140441271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDL2136381.1"
                     /translation="MEANHQRNDLGLVALTMLAQYHNISLNPEEIKHKFDLDGKGLSL
                     TSWLLAAKSLALKAKHIKKEISRLHLVNLPALVWQDNGKHFLLVKVDTDNNRYLTYNL
                     EQDAPQILSQDEFEACYQGQLILVTSRASVVGQLAKFDFTWFIPAVIKYRKIFLETLI
                     VSIFLQIFALITPLFFQVVMDKVLVHRGFSTLNIITVALAIVIIFEIVLSGLRTYVFS
                     HSTSRIDVELGAKLFRHLLSLPISYFENRRVGDTVARVRELDQIRNFLTGQALTSVLD
                     LLFSFIFFAVMWYYSPKLTLVILGSLPCYILWSIFISPILRRRLDDKFARSADNQAFL
                     VESVTAINMIKAMAVAPQMTDTWDKQLASYVSSSFRVTVLATIGQQGVQLIQKTVMVI
                     NLWLGAHLVISGDLSIGQLIAFNMLSGQVIAPVIRLAQLWQDFQQVGISVTRLGDVLN
                     SPTEQYQGKLSLPE"
BASE COUNT         3658 a         2273 c         2398 g         3401 t
ORIGIN      
        1 gcaaggtttt tttcggtttc ttggcaggga agctatccca cgcttgcatt gcattcgttt
       61 tacaattttc ttgtagcggg cagagcaagc acttaggctt tgtacgggtg caaatcatcg
      121 cccctaaatc catcatcgct tggttaaagt ctgccacttg ggtatcaggc gtcacacttt
      181 cgcttaattg ccaaaggcga ttttccaccg ctttttcacc cgcccagcct tctactgcaa
      241 aatagcggct taacacccgt ttgacattgc catctaaaat cgggtgtggg gcatctaaca
      301 cagaagacaa aatcgcccct gcagtacttc tgcccactcc ggttaatgcc aacacatcat
      361 caaatcgggt aggaaattca ccgccaaact catctcgaat ttgaattgcc gctttatgca
      421 aatttcttgc ccgagcgtaa taacccagcc ctgtccacaa atgcaatact tcatcaatcg
      481 aggcattagc taagtcaatt acggtgggaa atcgttccat aaaacgtgca aaataaggaa
      541 taacggtcgc tacttgagtt tgttgcaaca ttacctccga caaccaaacc tgatacagcg
      601 ttttattttg ctgccaaggt aaatgtttcc gcccatattg ctcaaaccaa gctaaaacag
      661 ctttggcaaa cggatcagaa gcttttgctt tcgctaaagg aaatggggaa ttgctgttca
      721 ttaaaaatct tgaaatccga aaaataaatt atcatactgc aagcggtcag ttttaccaaa
      781 aattttacac attaaggaaa aaagatgaaa tcgcccattt taggtttcca ccacattgca
      841 ttaattgttt ccgattacga aaaatccaaa catttctaca cccgcatttt aggggcagag
      901 atcatcgcag aaacctaccg agagcaacgt gccagctata aattagattt acgctttaaa
      961 gatggctcac aaattgaatt attcagtttt cctgaaacgc cgcctcgttt cacctcgccc
     1021 gaaacctgcg ggcttcgcca tttggcattt cgagtggaaa atattgaata tgccatcgca
     1081 tttttagaac aacatcaatt accgcatgaa gggatccgaa ttgatgagat caccggcaaa
     1141 cgcttcacgt ttttccgaga tccggacgat ctgccgttag agttttacga ggtctaaatg
     1201 ttatatttac tgattgcaac cctgatttgg gcgagttcat tcattgtagg taaagtggcg
     1261 atttcagcct ttgaccctgt ccaaattatg caaattcggc tgacgttagc ggcgtttatc
     1321 gctctgccgt tttttattcc tgcgtataaa aaaatccaga aaaatcagca cattaaagtc
     1381 tggctgatta gttttctcac ttttcctctg tgcgtgatct tacaattttc cggcttgaaa
     1441 ttaacttccg cagcaagtgc cgtggcacta ctcgggctaa acccgctttt aactgtgtta
     1501 gtcagctatt tttgtttcaa aaaacaggca accaggctcg attttatcct aagtttattt
     1561 gcctgcttgg ggattttaat tatggcatcg ggtagcgatg atagcggttc aatcaatgta
     1621 ttaggattat tgcttgtaac cttaggcagt ttaagtttta ttgtgggaat gtacctcagc
     1681 aaagagattc tgcaatcagt aaaagtgatc gatttaacca agatcacgct ggtgcaaggg
     1741 gcaattaccg ccttacctat taccttactc tttgcagaaa attggcaact gccaaccagt
     1801 ctctcagcat gggcgagcgt agcatattta ggcattattt gtaccagctt ggcaatattg
     1861 ttatggaaca aaggcatcag ccaatcttcg cctattcttg caggtattct actcgcactt
     1921 gaaccggtat tcggactgct aatggcattg gtctttttat cagaacagct cagtttagtg
     1981 acttgggttg ggattattat tgttatttgc accgccttac tctccgtttt gctaccgatg
     2041 atgaaaagat acaagcggtt aatttaacag gattttttgc aaatgaaatt tccccctctt
     2101 tcccacggca ttttacttcg ccgttataaa cgttttcttg ccgatattca gttgccaaat
     2161 ggcgaacaaa ttaccctcca ttgcccaaat accggtgcga tgacaggctg tgccaacccc
     2221 ggtgatacag tttggttttc cacctctcat aaccccaaac gtaaatatgc tcacacttgg
     2281 gaactaaccc aaacccaagc gggggatttt atttgtgtca ataccttgag agccaatcag
     2341 cttgtacaag aagccttgga aaatcaatgg attagcgagc ttgcggatta tcagcacatt
     2401 ctgcctgaac aaaaatatgg cagtgaaaac agtcggattg attttttgct caaatcggac
     2461 cttctgccgg attgttttgt ggaagtgaaa tctaccaccc ttttaaccga aaacggtgtg
     2521 ggaatgttcc ccgatgccaa aaccgagcgg gggcaaaaac atttgcggga gctgacgaaa
     2581 atcgctgaac agggcttaca agcggtcatt tttttcgcta ttttgcacac aggtattgaa
     2641 cggtttgaag tggcaaagca gatagacccc aaatatgccg aattattcgc tcaagccatt
     2701 gcacaaaaag tgcaagttct ctcctataaa atggcaattt cattggaaaa cgggcaacct
     2761 attgcgacaa ccttaacaaa gaaatgtgag ttttaagctt gaaaacacac agcatatccc
     2821 catataagcc aaagttttta aaaagaagga tttagtatga caacgattgt atgcgtgcgt
     2881 aaagacggaa aagtggctat cggtggtgat ggtcaggcaa cgcttggaaa ttgtatcgag
     2941 aaaggcacgg tgcggaaagt tcgccgtttg tataaagaca aagtggtgac aggctttgcc
     3001 ggctcgaccg ctgatgcctt tattttgcgt gatttattcg agaaaaaatt ggagttacac
     3061 caagggcatt tagtgaaatc ggcagtggaa ctggcaaaag agtggcgtac cgagcgtgct
     3121 ttacgccgtt tagaagcgat gatgattgtc gccaatgaca gcgagttttt attggtttca
     3181 ggttcgggcg atgtgattga gccggagcaa gatgtactgg cgattggttc aggcggtaac
     3241 tatgcgaaag ccgctgctct tgcgttatta cgcaccgaaa ataatttatc tgcaaaagaa
     3301 atcgttgcgg aagcgttaaa aattgccggc gatattgata tttacagtaa ccacaagcac
     3361 gtgattgaag aagtttagtt aaataactct ctccccttgt gggagagggt aattaattta
     3421 aggaataaca ttatgtcaat gacaccccgt gaaatcgtct cagaattaga cgcacacatt
     3481 atcggacaaa aagatgcaaa acgtgcggta gcaattgctt tgcgtaaccg ctggagaaga
     3541 atgcagttgc cggaagatct ccgccaagaa gtgaccccca aaaatatttt aatgatcggt
     3601 ccgaccggtg tgggtaaaac cgaaatcgcc cgccgtttgg cgaagcttgc caatgcccct
     3661 tttatcaaag ttgaggcaac caaattcact gaagtggggt atgtgggtaa agaagtggat
     3721 tcgattatcc gtgatctagc tgatgtgtcg atgaaattag tccgccaaca agcggtcgaa
     3781 aaaaataaaa tgcgtgcaca agatgcagcg gaagatcgca ttttagacgt attattgcca
     3841 ccggcgaaag atcagtgggg aaatgtgcag gaatccgaca ataattctac ccgccaaact
     3901 ttccgcaaaa aattgcgtga agggcaatta gacgataaag agattgaaat tgacgtggca
     3961 gctcaagtga gcgttgagat tatgaccccg ccgggaatgg aagagatgac ctcgcaatta
     4021 caatcgctgt ttgagggaat gtcgccaagc aaaaccaaaa aacgcaaaat gaaaatcaaa
     4081 gatgcgttaa aggtgatgct tgatgaagaa gcggcgaaat tagtaaatcc tgaagagctg
     4141 aaacagcaag cgattgaagc ggtggaacag cacggtattg tctttattga tgagatcgac
     4201 aaaatctgta aaaaaggcga gcattcgggt ggagatgttt ctcgtgaagg tgttcagcgt
     4261 gatttacttc caatcattga aggctcaacg gtaaatacca agcacggtat ggtgaaaacc
     4321 gaccatattc tgtttatctg ctcgggggca ttccaagtgg ctcgcccgtc tgatttactc
     4381 ccagaattac agggtcgctt gccgattcgt gtggagttaa aatcgcttac caaagaagat
     4441 tttgagcgta ttttaaccga accgaatgcc tcattaacct tgcaatatcg tgagttgatg
     4501 aaaactgaag gcgtggagat tgagttcact aaaggcggta tcagccgtat tgcagaaagt
     4561 gcattccgtg tgaacgagaa aaccgaaaac atcggggcaa gacgtttaca caccgtgctt
     4621 gagcgtttga tggacggcat ttcgttcgat gccagcgaac gccgtggtga aaaaatcatc
     4681 attgatgaaa actatgtttc cgaagcgtta aatgacgtgg tggaaaacga agatttaagt
     4741 cgctttattt tgtagtttta agttatacaa gcggttattt tagcgaaaaa tcttgcgaat
     4801 tagccgcttt tcatattgtt agttgtaaaa atgaaaaatc tatacaaatg gcctgctgca
     4861 caatcgcttt atccaaatca gggaaagaag tcttacgcag ggaaacgttt tcgttaccgt
     4921 cttcgctctt ggttgcatca ttccaaaatc aagcaatttg agcagttcgt tacacaaaat
     4981 ccacaactga ttccgctctt aaatgctcga ccaaattaca gctaccccgt ggctcaccgc
     5041 tttttagata agcgtttcag tgcaaaacaa cgcttacaaa aaatcacaga taacctgctg
     5101 tttttaccgc aaaaattggc tcatttacca cctctatggg aacaagcggt caattttggt
     5161 gaaattattg cagattttga gttatggctt aacatcaatg aacatcagcc gatggaaggt
     5221 ttttgggcat tagaacttcg ccacagaccg actaatcagc tagtttattt attaacattt
     5281 ggtaagttag atgaagcctt gttgatagcc gtaattcaag gtccgaattt tgaagattca
     5341 aaagagctgg tgaaacaatt aaccaaaagt tgccacggct tacgcccggc ctatttaatg
     5401 gttgaaacga tgaaggcttt aacggctgtt cttggctatc agagtttact aggcattcca
     5461 cagaaatacc agaataaatc ccgctgggtg caaagtaagc gctatgtagt ggattatgat
     5521 gctatttttg cggaatcggg cggaaagccg ggaaattatt ggcagttacc ccttgaattg
     5581 gaaagtaaaa aaatagatga aattccaagc aaaaaacgct caatgtatcg caagcgttat
     5641 gaaatgctag ctaatttaaa gcagaccatg gagcttatgc ttaaaactta tactggctta
     5701 aatgggtaaa tcaaagaata caagcggtca aaatctgcaa aactttgcaa aaaatgaccg
     5761 cttacaagtt acttgccgcc catccattta tcatagattt tttgatattc gccattctct
     5821 ttaatcgttt ttaatgctgc attgaattga tcaactaata cttggttttt caagtgagtt
     5881 gcaatgccta aaccatcacc aaaatagttt ggattattga ttttctcccc tacaaaatct
     5941 agcccttcgt gttttcttgc catctcagcc aataccggca catcgccaaa aatagcatca
     6001 attcgcccat tttgcacatc taaaatagca ttttgataag aggcataaga tttcacattg
     6061 tactcttttt tctcttttgc taaatagtgt tggtaagtag tcccattttg cacaccaatc
     6121 gttttcacct tagcaaaatc tatatctttt ttcgcaatga aggcagcaga gcttggaaag
     6181 taaggctcgc taaataatac ttgtttctta cgtggttccg caatacccat acctgaaatt
     6241 gcagcatcaa attgtttttg ttttaggctt tggattaagc tatcaaaagg ttggctatgg
     6301 aatgtacaat ttgcattcat ctctttacag atagcatttg caatatccac atcaaaaccg
     6361 ataatttctc ccttctcgtc ggtcatttca aatggaggat agcttggctc catcacaaat
     6421 ttgatatctt gtgcctgcgc agtaaccaca cacccgaata aaagggtcaa aagtgttttt
     6481 ttcataaaaa gtccctgtgt tttcattata aggattacca ctttaacgca gttactttct
     6541 taaaaaaagt cttcttttca taaagtttgt tttatgtcat acaaacacat caaattgaga
     6601 tgtagtttct caatcctctt gattcctcta tctcaaaaaa acaaccaaaa agaaaaaaga
     6661 aaagtatatg ttacattaat attacaatgt aattattttg tttaatttcc ctacattttg
     6721 tataacttta aaacactcct ttttctcttc tgattatata aaagacaaaa aatacaattt
     6781 aagctaaaaa aaacaacaaa aaatacgaca ataagatcga gtaatgatta tattatgcta
     6841 taatttttga cctaatttag aataattatc gagtgcaaat tatgaatcaa tcttatttta
     6901 acttactagg aaacattact tggctatgga tgaactcctc cctccacaaa gaatggagct
     6961 gtgaactact agcacgcaat gtgattcctg caattgaaaa tgaacaatat atgctactta
     7021 tagataacgg tattccgatc gcttattgta gttgggcaga tttaaacctt gagactgagg
     7081 tgaaatatat taaggatatt agttcattaa caccggagga atggcagtct ggcgatagac
     7141 gttggattat tgattgggta gcaccattcg gacattctca attactttat aaaaaaatgt
     7201 gtcagaaata tgctgataca ctcgtcagat ctatacgctt ccaaccaaat caaaaatcag
     7261 taggaaaaat tgcttatttc aaaggaggta aattagataa aaaaacagca aaaaaacgtt
     7321 ttgatacata tcaagaagag ctggcaacag cacttaaaaa tgaatttaat tttattaaaa
     7381 aatagaagga gacatccctt atgggtaata aacttactaa tatttcaaca aatttaaaaa
     7441 gttcctggtt gactgctaaa tccggtttaa atagaacagg acaatcatta gctaaagcag
     7501 ggcaatcttt aaaaacaggg gcaaaaaaaa tcatcctcta tatcccaaaa gattaccaat
     7561 atgataccga taaaggtaat ggtttacagg atttagttaa ggcagctgaa gagcttggta
     7621 ttgaagtgca aaaagaagag agcaatgata ttgcaaaagc acaaaccagt ttaggcacaa
     7681 ttcacaatgt attgggttta actgagcgtg gtattgtttt gtcagcccct cagcttgata
     7741 aattgcttca aaaaaccaaa gtaggtcaag cgattggctc tacagaaaat attacaaaag
     7801 gttttagtaa tgcaaaaaca gtattatctg gaatccaatc tattctaggt tctgtcttag
     7861 ccggtatgga cttagatgaa gcattacaaa ataacagcaa tgagctaaca cttgctaaag
     7921 ctggtttaga gttaaccaac tcgttgatcg aaaatattgc taactcagtg aaaacattag
     7981 acgcctttgg tgatcaaatt aatcaacttg gttcaaaact gcaaaatgtc aaaggattaa
     8041 gctctcttgg cgaaaaacta aaaggtctga gtggctttga taaaaccagc cttggtttag
     8101 atattgtatc cggcttgctg tcgggagcaa cggctgcact tgtacttgca gacaaaaacg
     8161 cttcaacatc aagaaaagtg ggtgcaggtt ttgaattagc aaaccaagtt gttggtaata
     8221 ttaccaaagc cgtttcttct tacattttag cccaacgtgt tgcagccggt ttatcttcaa
     8281 ccggacctgt ggcagcctta attgcttcta ccgtttctct tgcgattagc ccattatcat
     8341 ttgccggtat tgccgataaa tttaatcacg caaaaagttt agaaagctat gctgaacgtt
     8401 ttaaaaaatt aggctatgac ggtgataatt tattagcaga atatcaacgt ggaacaggga
     8461 ctattgatgc ttcagtaaca gcaatcaata cagcattggc tgctattgct ggtggtgtat
     8521 ctgccgctgc tgccggctct gtggttgctt caccaattgc cctacttgta tcaggtatta
     8581 ccggtgtgat ttccactatt ctacagtact ctaaacaagc aatgtttgaa cacgttgcaa
     8641 ataaaattca caataaaatt gtagaatggg aaaaaaataa tccaggtaag aactactttg
     8701 aaaatggtta cgatgcccgt tatcttgcga atttacaaga taatatgaaa ttcttactga
     8761 acttaaacaa agagttacag gcagaacgtg ttattgcaat tactcagcag caatgggata
     8821 acaacattgg tgatttagcc ggtatcagtc gtctaggtga aaaagtactt agcgggaaag
     8881 cctatgttga tgcctttgaa gaaggtcaac atcttaaagc ggataaatta gtgcaattag
     8941 attctgcgaa agggattatt gatgttagta acactggaga agcgaaaaca cagcatattt
     9001 tattccggac accgttatta actccgggaa cagaaaaacg cgagcgagtg cagacgggta
     9061 aatatgagta tattactaaa cttcatatta accgagtaga tagctggaaa attacagatg
     9121 gtgcagcaag ttctaccttt gatttaacta acgttgttca gcgtattggt attgaattag
     9181 acaatgctgg aaatgtaact aaaaccaaag aaacaaaaat tattgccaaa cttggtgaag
     9241 gtgatgacaa cgtatttgtt ggttctggta cgacggaaat tgatggcggt gaaggttacg
     9301 accgagttca ctatagccgc ggaaactatg gtgctttaac tattgatgca accaaagaga
     9361 ccgagcaagg tagttatacc gtaaatcgtt tcgtagaaag cggtaaagca ttacacgaag
     9421 tgacttcaac tcacaccgca ttagtgggca accgtgaaga aaaaattgaa tatcgtcata
     9481 gcaataacca gcaccatgcc ggttattaca ccaaagatac cttgaaagct gttgaagaaa
     9541 ttatcggtac atcacataac gatattttta aaggtagtaa gttcaatgat gcctttaatg
     9601 gtggtgatgg tgtcgatact attgacggta acgacggcaa tgaccgctta tttggtggta
     9661 aaggcgatga tattatcgat ggtggaaatg gtgatgactt tatcgatggc ggtaaaggca
     9721 atgacctatt acacggcggt aaaggtgatg atattttcgt tcaccgtcaa ggcgatggta
     9781 atgattcaat tactgaatcg gaaggtaatg ataaattatc attctctgac tcaaatctaa
     9841 aagatttaac ctttgagaaa gtaaatcacc accttgtgat tactaatacc aagcaggaaa
     9901 aagtcaccat tcaaaactgg ttccgtgaag cagagtttgc aaaaacaatt caaaattatg
     9961 ttgcaacaag agacgataaa attgaagaga ttatcggtca aaatggtgaa cggattacct
    10021 cgaagcaagt ggatgaactt atcgaaaaag gtaacggtaa aatcgctcag agtgaactga
    10081 caaaagttgt ggataactat caattactca aatatagccg agatgcatca aacagtttag
    10141 ataagttaat ctcatctgca agtgcattta cctcgtctaa tgattcgaga aatgtattag
    10201 cgagcccaac ttcaatgttg gatccgagtt tatcttctat tcaatttgct agagcagctt
    10261 aatttttaat gattggcaac tctatattgt ttcgcacatt atggagttgc cgttttattt
    10321 tataaaagga gacaatatgg aagctaacca tcaaaggaat gatcttggtt tagttgccct
    10381 cactatgttg gcacaatacc ataatatttc gcttaatccg gaagaaataa aacataaatt
    10441 tgatcttgac ggaaaagggc tttctttaac ctcttggctt ttagccgcaa aatcgttagc
    10501 gttgaaagcg aaacacatta aaaaagagat ttctcgctta cacttggtga atttacctgc
    10561 attagtttgg caagataacg gtaaacattt tttattggta aaagtggata ccgataataa
    10621 ccgctattta acttacaatt tggaacaaga tgccccacaa attctgtcac aagacgaatt
    10681 tgaagcctgc tatcaagggc aattaatttt ggtcacatct agagcttccg tagttggtca
    10741 actagcaaag ttcgatttca cctggtttat tccggcggtg atcaaatacc gaaaaatctt
    10801 tctagaaacc ttgattgttt cgatcttttt gcaaattttt gccctaatta caccgctatt
    10861 tttccaagtt gttatggata aagtactggt gcatcgaggt ttttcaacct tgaatatcat
    10921 tacggttgcc ttagctattg tgatcatctt tgaaattgtc ttaagcggtt tacgcaccta
    10981 cgtgttttcc catagcacta gtcggattga cgtggaatta ggcgctaaat tatttaggca
    11041 tttattatcg ttacctattt cttattttga gaacagacgt gttggcgata cagttgcacg
    11101 ggtcagagaa ttggatcaaa tacgaaattt ccttaccgga caagcattaa cctcggtatt
    11161 agatttatta ttctctttta tcttttttgc cgtaatgtgg tattacagcc cgaaattgac
    11221 cttagtcatt ctcggctcac taccctgcta tattttatgg tcaattttta ttagtccgat
    11281 tttaagacgg cgtttagacg ataaatttgc ccgaagtgct gacaatcaag cattcttagt
    11341 tgaatcggta acagccatca atatgattaa agcgatggcg gttgctccac aaatgacgga
    11401 tacatgggat aaacagctgg caagctatgt ttcatcaagc ttccgtgtca ccgtattagc
    11461 aaccattggg caacaaggtg tacaacttat tcaaaaaacc gttatggtga tcaacctttg
    11521 gctaggggca cacttagtta tttcaggcga tcttagtatt gggcaattaa ttgcctttaa
    11581 tatgctatca gggcaagtga ttgcaccggt gattcggctg gctcagctct ggcaagattt
    11641 ccaacaagtt gggatttccg tcactcgctt aggtgatgtt ttaaactctc caaccgaaca
    11701 atatcaaggc aaattatcac taccagaaat
//
LOCUS       DANKBV010000068        11253 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.068, whole genome shotgun sequence.
ACCESSION   DANKBV010000068 DANKBV010000000
VERSION     DANKBV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.068"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            57..920
                     /locus_tag="PWY43_001512"
     CDS             57..920
                     /locus_tag="PWY43_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL2136382.1"
                     /translation="MIYSMTAFAHLEMKKEWGNAVWEIRSVNQRFLETYFRLPEQFRN
                     LEMTLRERLRSALTRGKVECSLRIDLSKTQNSEIALNKEYAAQVVQSLKWLKETANEG
                     EINLVDVLRFPGVVDNESQDLDQIAQDLLAGFEQILADFIAMRAREGENVQALILQRL
                     EAISVEAAKVQAQMPEILQWQKDRLQQRFDELNLQLDPQRLEQEMVLLAQRVDVAEEL
                     DRLQLHVKETTNILKKGGAVGRKLDFMMQELNRESNTLASKSINADVTNSAVELKVLI
                     EQMREQIQNLE"
     gene            929..1819
                     /locus_tag="PWY43_001513"
     CDS             929..1819
                     /locus_tag="PWY43_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HDL2136383.1"
                     /translation="MAMNFKQVSQSYPNLCVPLSQYCLIEIAGVDAEKYLQGQLTCDV
                     AKISVGEHTLTSHCDPKGKMSALLRLYRAENEKFMAIIHRSLLPEALTQLKKYAVFSK
                     VTFTELDIPLYGMAGEAAFAKLSENMTALRLTTGQPRAIVWGEELETTADEQLWTLMD
                     IQDGIPVLLQQNQFELIPQATNLQALESAISFTKGCYIGQETVARAKYRGANKRALFT
                     LAGNFESEISLPEVASAIEMQLGENWRATGTVLTSATDENTLWVQVVLNKDVEPNTKF
                     RANGVALEMVELPYSLAENE"
     gene            complement(1851..2111)
                     /locus_tag="PWY43_001514"
     CDS             complement(1851..2111)
                     /locus_tag="PWY43_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HDL2136384.1"
                     /translation="MALLITHKCTNCDMCLPECPNEAISVGDDIYIIDPVLCTECVGH
                     YEKPTCQKVCPITNCIIKDPDNIETEEQLWERFVMIHHPDQI"
     gene            2341..2760
                     /gene="rbsD"
                     /locus_tag="PWY43_001515"
     CDS             2341..2760
                     /gene="rbsD"
                     /locus_tag="PWY43_001515"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL2136385.1"
                     /translation="MKKTLNLNAQLSHSIATLGHTDSLTVCDAGLPIPNTIERIDLAL
                     TAGVPSFLQTFDVVVSELFVERVLLAEEIKHKNPQVLAALLERLTQLEQAQQNKIQID
                     YVNHEIFKEQTHYSKAVVRSGECSPYANIILYSGVPF"
     gene            2770..4266
                     /gene="rbsA"
                     /locus_tag="PWY43_001516"
     CDS             2770..4266
                     /gene="rbsA"
                     /locus_tag="PWY43_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDL2136386.1"
                     /translation="METLLKMSGIDKEFPGVKALSNACLSVYGGRVMALMGENGAGKS
                     TLMKVLTGIYSKDAGSISYLGKEVTFKGPKDSQEAGISIIHQELNLVGNLAIAENIFL
                     GREFKTAWGAIDWKKMYAEADKLLARLGVKHSSHQLCAELSIGEQQMVEIAKALSFES
                     QVIIMDEPTDALTDTETASLFKVIRELKAENRGIVYISHRLKEIFEICDDVTVLRDGQ
                     FIGERVIADIDEDQLIEMMVGRRLEEQYPHLHQERGELLLEVKNLSGSGVNNVSFQLH
                     KGEIVGISGLMGAGRTELMKVLYGALPKTAGSIELNGKAISNHCPQDGLNNGIVYISE
                     DRKGDGLILGMSVKENMSLTSLDHFSKAGSIRHDAEKLAVDDFIEMFNIKTPSREQQI
                     GLLSGGNQQKVAIAKGLMTRPNVLILDEPTRGVDVGAKKEIYQLINEFKKDGLSILMV
                     SSDMPEVLGMSDRILVMHEGQISAEFSRAEATQEKLLAEAIGKSTGLN"
     gene            4276..5244
                     /gene="rbsC"
                     /locus_tag="PWY43_001517"
     CDS             4276..5244
                     /gene="rbsC"
                     /locus_tag="PWY43_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDL2136387.1"
                     /translation="MATQTKQFQLGRFLIEQRSFVALFILIVIVSMINPDFFSVDNIL
                     NILRQTSVNAIIAVGMTFVILIAGIDLSVGSVLALTGAVAATLVGSELPILLVIPLVL
                     LLGTAFGGISGAIVAKGKVQAFIATLVTMTLLRGITMVYTDGRPISTGFSDQADAFAF
                     IGTGYLFGIPVPIWIMAVVFGLAWYILKHTPIGRYIYALGGNESATQLSGINVNKIKI
                     FVFAVSGFLSALAGLIVTSRLSSAQPTAGVSYELDAIAAVVVGGTSLMGGKGRVMGTL
                     VGALIIGFLNNALNLLDISSYYQMIAKALVILAAVLADNYLGTKKV"
     gene            5267..6145
                     /gene="rbsB"
                     /locus_tag="PWY43_001518"
     CDS             5267..6145
                     /gene="rbsB"
                     /locus_tag="PWY43_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDL2136388.1"
                     /translation="MKKLTTIASAVMLSLSVAATASAKETIALAVSTLDNPFFVSLKD
                     GAQKKADELGYKLVVLDSQNDPAKELSNVEDLTVRGAKVLLINLTDSEAGGNAVAIAN
                     RNKIPVITLDRGAAKGEVVSHIASDNVAGGKMAGDFIAQKLGSGAKVIQLEGIAGTSA
                     ARERGEGFKQAIEAHKFNVLASQPADFDRTKGLNVTENLLSSKGDVQAIFAQNDEMAL
                     GALRAVSSAKKEVVIVGFDGTDDGVKAVESGKLAATIAQQPELIGSLGVETADKVLKG
                     EKVEAKIPVDLKVISK"
     gene            6266..7195
                     /gene="rbsK"
                     /locus_tag="PWY43_001519"
     CDS             6266..7195
                     /gene="rbsK"
                     /locus_tag="PWY43_001519"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL2136389.1"
                     /translation="MKKLTVLGSINADHVISVPYFAKPGETLTGHSCHIAYGGKGANQ
                     AVAAARLGAKVSFIGCIGSDDIGKAMKSAFEQDGIDTRSIKTIPNEMTGIAMIQVAES
                     GENSIVISAGANGHLDETVVAKCAAEITQADYLLMQLETPLPAIVQAAKLAQQNGTKV
                     ILNPAPARALPDELLNLLDMITPNETEAEILTGVKVVDEASAIQAAQVFHQKGIEKVL
                     ITLGSKGVFVSENGVGKIVAGFRVQAVDTTAAGDTFNGALVTAMLEDKPLDQAIRFAH
                     AAAAISVTRKGAQPSIPTRQETLDFLSQQAVSF"
     gene            7209..8219
                     /locus_tag="PWY43_001520"
     CDS             7209..8219
                     /locus_tag="PWY43_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2136390.1"
                     /translation="MATMKDIARLAQVSTSTVSHVINNSRFVSEEIRERVQRVVKELN
                     YTPSALARSLKVNETKTIGMLVTATGNPFFAEVMAGVEQYCQQHQYNLIIATTNGDTE
                     RLQHHLQMLMQRKVDGLLLMCGDTRLNADESLNIPLPLVVLDWWFTELNADKIFENSI
                     SGGYLATKTLIEAGHSKIAIITGNLKKSLAKNRLEGYKKALAEANIPLNPNWVFESHF
                     DFAGGVNGMNALLAQSEKPTAVFACSDTIALGAYQAIWQQGLRIPQDISIIGYDDIEL
                     AKYLSPPLSTISQPKAELGRLAVETLLKRIRFKSTDFQTIMLEPELVLRHSIQTINKN
                     AE"
     gene            complement(8255..8884)
                     /locus_tag="PWY43_001521"
     CDS             complement(8255..8884)
                     /locus_tag="PWY43_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2136391.1"
                     /translation="MTAPTQILLIDDHPLMRQGMRQLLELDHQFSVVGEASNGTDGIK
                     LALRLEPDVIILDLNMKGLSGLDTLRSLRAQGIDARIIVLTVSDEQSDVFALMDAGVD
                     GYLLKDTDTPELLDNIRKAAAGEVVLSETVRQHLLNRQPENDPLSCLTDRELDVLQWL
                     ATGMSNKQIAAQLFISEETVKVHIRNLLRKLNVHSRVAATVLYLEQQKG"
     gene            9082..11241
                     /gene="rlmKL"
                     /locus_tag="PWY43_001522"
     CDS             9082..11241
                     /gene="rlmKL"
                     /locus_tag="PWY43_001522"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDL2136392.1"
                     /translation="MTNQTTYFATAARGFEEILKTELEQICAAECKVAQGGVHFTTTQ
                     KGAYQALLHSRLASRILLPLISTKIFSDSDLYASIVSFNWADLFDPRDTFYIDFSGTN
                     REIRNTQFGAMRVKDGIVDYFERKGFARPTVDKDNPDVRIHVYLDRENVVVSLDLSGD
                     ALHIRGYREETGKAPLRETLAAAIILRSGWKVGTPLVDPMCGSGTLLIEAAQMAANIA
                     PQLHRKHWGFNAWKGHQQAMWKAVLDEAFRNVELGAVGENCNSSLQKMFFGFDLDHRV
                     LTKAKQNAKNAGVDHLIQWQQGDVAALKNPIPEKIGTVVCNPPYGERLGTTPALIALY
                     SVFGQRLKQQFAGWNASIFSGEPELLNCLRLRSARQFKAKNGPLDCVQKNYSISEHNK
                     RSDLDENLQFEQNAQVAPDFANRLTKNIKKIEKWAKQQGIDAYRLYDADLPEYNLAVD
                     RYGDHIVVQEYQAPKNIDEQKARQRLLDAVSATLYVTGVETNKLVLKVRQKQKGTNQY
                     EKLANKGEYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGEMAKGKTFLNLFAYT
                     GSATVHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLSLRHHRLFQADCLQWLAECRE
                     RFELIFVDPPTFSNSKRMENSWDVQRDHIELMKQLKRILTADGTIVFSNNKRGFKMDF
                     DGLAALGLTAENISHKTLPLDFERNPQIHNCWIVKNV"
BASE COUNT         3328 a         2158 c         2687 g         3080 t
ORIGIN      
        1 caaggggaga gagctttact tataaaaaca caggaatacc aacacaagga ataccaatga
       61 tttatagtat gacagcattt gcccatttag aaatgaaaaa agagtggggc aatgcggtgt
      121 gggaaattcg ctcggtaaat caacgctttt tagaaaccta ttttcgtttg ccggagcagt
      181 ttcgcaacct agaaatgacc ttgcgtgagc gtttacgttc tgcactgaca cgaggcaaag
      241 tagagtgtag cttacgcatt gatttaagca aaacccaaaa cagtgaaatt gcgttaaaca
      301 aagaatatgc cgcacaggtg gttcaatcat taaaatggtt aaaagaaacc gcaaatgaag
      361 gcgaaatcaa ccttgttgat gtgctacgtt tcccgggcgt ggtggataat gaaagccaag
      421 atttagacca aattgctcaa gatttattag ccggctttga acaaattctg gcagatttta
      481 tcgcaatgcg agccagagag ggcgagaacg tgcaagccct gatcttacaa cgtttagaag
      541 caatttcagt tgaggctgca aaggtacaag cccaaatgcc ggaaattctg caatggcaga
      601 aagatcgttt acaacaacgg ttcgatgaac tgaatttaca gcttgatcca caacgcttag
      661 aacaagaaat ggtgttactt gcccaacgtg ttgatgtggc ggaggaatta gaccgcttgc
      721 aattacacgt taaagaaacc accaatatcc tgaaaaaagg cggtgcggtt gggcgtaaac
      781 tggattttat gatgcaagaa ctaaaccgtg aatccaacac gctcgcctca aaatcgatta
      841 atgcggacgt gaccaattcg gcggtggagc tgaaagtgtt gatcgaacag atgcgtgaac
      901 aaattcagaa tttagaatag gacagattat ggcaatgaat ttcaagcaag tgagccagag
      961 ctatccaaat ctctgcgtgc cgctttctca gtattgttta attgaaatcg caggggtaga
     1021 tgctgagaaa tatcttcaag ggcaattaac ctgcgatgtg gcaaaaattt cggtgggcga
     1081 acatacgcta accagccatt gtgatccgaa aggcaaaatg agtgcgttac tgcgtttgta
     1141 tcgtgcagaa aatgagaaat ttatggcgat cattcaccgc agcttattgc ctgaagccct
     1201 gacgcaactt aaaaaatatg cggttttttc aaaagtgacc ttcacggaat tagacatccc
     1261 actttatggt atggcaggtg aagcagcatt tgcaaaatta agcgaaaata tgaccgcttt
     1321 gcgtttaacc acaggacagc ctcgggccat tgtttggggc gaagagcttg aaacgactgc
     1381 cgatgaacag ctctggacat taatggatat tcaagacggc attcctgttc tcttgcaaca
     1441 aaaccagttt gagctgattc cacaggcaac caatttacag gcacttgaat ctgcgatttc
     1501 attcaccaaa ggttgctaca tcgggcaaga aacggtagcg agagcgaaat atcgtggggc
     1561 gaataaacgt gctttattta cccttgccgg caactttgaa agcgaaatca gcttaccgga
     1621 agttgcttct gcgattgaaa tgcaactggg cgaaaattgg cgagcaaccg gcactgtgtt
     1681 gaccagtgcg actgatgaaa ataccctgtg ggtgcaagtg gtgctgaata aagatgttga
     1741 acctaacacc aagtttagag ccaatggtgt agcgttggaa atggtcgagt taccttatag
     1801 tttggcagaa aatgagtaaa acgaacggtc atcagagtat gaccgttttt ttatatctga
     1861 tcagggtgat gaatcatcac aaaacgttcc cacaactgct cttcggtttc gatattatca
     1921 ggatctttga taatgcagtt ggtaatcggg caaactttct ggcaagtcgg cttttcataa
     1981 tgcccgacac attcggtaca taacaccgga tcgatgatat aaatatcatc gcccaccgaa
     2041 atcgcctcgt tcgggcattc cggcaagcac atatcacagt tggtacattt gtgggtaatt
     2101 aataaagcca tagtttatat tgagaaaata aaaattaaac aagcggtcga ttttgcaaaa
     2161 aaatttgcaa atcttaccgc ttgtcattaa atgctaccta ttataaagca aaataatttg
     2221 aactacttca catttttgca atttggttgt ttatccgatt aagaaaattt tttatgatgc
     2281 catcgaaacg tttcgatgga gtattttcca acgtgatgga gcgaaaataa aggggttagt
     2341 atgaaaaaaa cgctaaatct aaatgcacaa ctttcgcaca gtattgcgac actcgggcat
     2401 accgatagtt taacggtttg cgatgccggt ttgccgattc caaacacaat agagcggatc
     2461 gatttggctt taaccgccgg tgtgccaagt tttttacaaa catttgatgt agtagttagt
     2521 gaattatttg ttgaacgagt gctacttgct gaagaaatta aacataaaaa tccgcaagtg
     2581 ttagcagctc ttttggagcg tttaacccaa ttagaacaag ctcaacagaa taaaattcaa
     2641 attgattatg tgaaccacga aatctttaaa gagcagacac attacagtaa agctgttgtg
     2701 cgttcgggtg agtgttcacc gtatgccaat attattttat attcgggcgt gccgttttag
     2761 ggtggaatga tggaaacctt attaaaaatg agcgggatag ataaagaatt tcccggcgtg
     2821 aaagccttga gcaatgcgtg cttatctgtt tacggtgggc gagtaatggc attaatgggc
     2881 gaaaatgggg caggtaaatc caccttgatg aaagtattga cggggattta tagcaaagat
     2941 gccggctcta ttagctattt aggcaaagaa gttaccttta aaggcccgaa agattcacaa
     3001 gaagccggta ttagcattat tcaccaagag ctgaatttag tcggtaattt agccattgct
     3061 gaaaatattt ttttgggccg tgagttcaaa accgcttggg gagctattga ttggaaaaaa
     3121 atgtatgccg aggcggataa actgcttgcc cgacttgggg taaaacacag tagccatcag
     3181 ctttgtgcgg agttatccat tggcgagcag caaatggtgg aaattgcgaa agccctgagt
     3241 tttgagtctc aagtaattat tatggacgaa ccgaccgatg ctttaaccga tacagaaaca
     3301 gcatcgttgt ttaaagttat tcgagagtta aaagcggaaa atcgaggcat tgtttatatt
     3361 tcccatcgtt taaaagagat ttttgaaatt tgtgatgatg tgacggtgct aagagacggg
     3421 caatttatcg gcgagcgagt aattgccgat attgatgaag atcagttaat tgaaatgatg
     3481 gttggtcgcc gtttagaaga gcaatatccg catttacacc aagagcgtgg cgagttatta
     3541 cttgaagtga agaatttaag tggaagtggg gtgaataatg tgtcattcca attacacaaa
     3601 ggtgaaattg tgggtatttc cggcttaatg ggggcagggc gtacagaatt aatgaaggtg
     3661 ctttatggtg cattgccgaa aacagccggc tcgatagagt taaatggaaa agcgatttcc
     3721 aaccattgtc cgcaagatgg gttgaataac ggcattgtgt atatttcaga agatcgtaaa
     3781 ggcgatggtc tgattttggg aatgtcggta aaagaaaata tgtctttgac ctctctcgat
     3841 catttctcca aagccggcag tattcgccac gatgcagaaa aattggcggt cgatgatttt
     3901 attgagatgt ttaacattaa aaccccaagt cgggagcaac aaatcgggct gctttccggt
     3961 ggtaatcagc aaaaagtggc gatcgctaaa gggttgatga cccgcccgaa tgtgctgatt
     4021 ttagacgagc cgacacgggg tgttgatgtg ggggcgaaaa aagagatcta tcaattaatt
     4081 aatgaattta aaaaagatgg gcttagtatt ttgatggttt cgtccgatat gccggaagtg
     4141 ttgggaatga gcgaccgcat tttagtgatg cacgaagggc aaatcagtgc agaattttca
     4201 cgagcagaag cgacacaaga aaagctactg gcagaagcta ttggcaaaag tacaggatta
     4261 aattaaggtg tgtttatggc aactcaaaca aagcagtttc aacttggcag atttttaatc
     4321 gagcaacgtt cttttgtagc gttatttatt ttaattgtca ttgtttcaat gattaaccct
     4381 gattttttca gcgtggataa tattttaaat attctacgcc aaacctcggt gaatgcgatt
     4441 attgcggtcg gaatgacctt tgtgatttta attgcaggca ttgatttatc ggtcggttcg
     4501 gttttagcct taaccggtgc ggttgcagcg accttagtcg gttctgaatt gccgattttg
     4561 ttggtgattc cgctggtttt attattaggg acggcatttg gtggtattag tggggcgatt
     4621 gttgctaaag gtaaagtgca ggcatttatt gcgactttgg tcacaatgac cttattgcgt
     4681 gggattacga tggtttatac cgatggcaga ccgattagca ccggcttttc tgaccaagca
     4741 gacgcctttg cgtttattgg gacaggctat ttatttggta ttcctgtgcc gatttggatt
     4801 atggcggtag tgtttgggtt agcttggtat attttgaaac acaccccgat tgggcgttac
     4861 atttacgctt taggtggcaa cgaatcggca acccagcttt caggcatcaa tgtgaataaa
     4921 attaagatct tcgtatttgc cgtgagtggt tttttatctg ctcttgccgg attaattgtg
     4981 acttcacgtc tctcttctgc tcagccaacg gcaggtgtgt cctatgagtt agacgcaatt
     5041 gcagcggtag ttgttggtgg aaccagtcta atgggcggta aagggcgtgt aatgggaacg
     5101 cttgtcgggg cattaattat cggtttctta aacaatgccc ttaatttatt ggatatttct
     5161 tcatattatc agatgattgc taaagcgttg gttattttag cagctgtatt agctgataat
     5221 tatctaggca ctaaaaaagt ttaatctatc tttaaaggag atttctatga aaaaattaac
     5281 tacgattgca tcagcggtta tgttaagttt atcggttgca gccactgctt cggcaaaaga
     5341 gactatcgct cttgcagtat cgactttaga taacccattc tttgtgagcc ttaaagacgg
     5401 tgcacagaaa aaagcagatg aattaggcta caaattagtg gtgcttgatt cccaaaatga
     5461 cccggctaaa gagctttcca atgtggaaga cttaacggtg cgtggggcaa aagtgttatt
     5521 gattaaccta accgattcgg aagcgggcgg aaatgcggtg gctattgcaa atcgcaacaa
     5581 aattccggtt attacacttg accgtggtgc agcaaaaggc gaagtggtta gccatatcgc
     5641 ttccgataac gttgccggcg gcaaaatggc gggagatttc attgctcaga aattaggctc
     5701 aggggcgaaa gtgattcaat tagaaggcat tgccggcact tccgccgctc gtgaacgtgg
     5761 tgaaggcttt aaacaagcga ttgaagcaca taaattcaat gtattagcca gtcaaccggc
     5821 tgattttgac cgcactaaag gcttaaatgt gactgaaaac ttgctctcaa gcaaagggga
     5881 cgtacaagct atttttgctc aaaatgatga aatggcacta ggagctttgc gtgcagtgag
     5941 ttcagcgaag aaagaggttg taattgtggg ctttgacggc acagatgatg gcgtaaaagc
     6001 cgttgaaagc ggtaaacttg ccgcaacgat tgctcaacaa cctgaactga ttggctcatt
     6061 aggcgtagaa actgccgata aagtgctaaa aggcgaaaaa gtggaagcaa aaattccggt
     6121 tgatttaaag gttatttcaa aataattata cttgaagata gcgttcgtct ctgacgaacg
     6181 ctttgaatta ataaaatagc actcgtcaga gactagtgct atcagaatcc tagttttgtt
     6241 gtactaggta atttgagggc ttaatatgaa aaaactcacc gttctcggta gcattaacgc
     6301 tgatcacgtg atttccgttc cctattttgc caaaccgggg gaaacgctca ccggacatag
     6361 ctgccacatt gcttatggcg gtaaaggggc aaatcaggcg gtggcggcag cacgtttggg
     6421 agctaaggtt tctttcatcg gttgtattgg cagtgatgac atcggcaaag caatgaaatc
     6481 agcctttgag caagatggca ttgacactcg ctcgattaaa acgattccga atgaaatgac
     6541 cggcattgca atgattcaag tggcggaatc gggcgagaac agtattgtga tttcagccgg
     6601 agcgaacggt catttggatg aaacggttgt ggcaaagtgt gcagccgaaa tcacacaggc
     6661 ggattatttg ctaatgcagc tagaaacacc gttgccggcg attgtgcaag cagcaaaatt
     6721 ggctcagcaa aacggcacga aagtaatatt aaaccctgcg cctgctcgag cgttacccga
     6781 tgaactgtta aacttactgg atatgattac cccaaatgaa acggaagcgg aaatattaac
     6841 cggcgtgaaa gttgttgatg aagccagtgc gattcaagcc gcacaagtgt ttcaccaaaa
     6901 gggcatcgaa aaagtgttga ttacacttgg ctcaaaaggg gtatttgtaa gcgaaaacgg
     6961 tgtaggcaaa atcgttgcag gcttccgtgt tcaagcggtg gataccactg ccgcagggga
     7021 tacttttaac ggagcattag tgactgcaat gttagaagat aaaccgctgg atcaagccat
     7081 tcgttttgct cacgcagcgg cagcgattag tgtgactcgc aaaggggctc aaccttctat
     7141 tccgacccgt caggaaacac tggattttct aagccaacaa gcggtgagtt tttaggaaaa
     7201 ttttgcaaat ggcaacaatg aaggatattg cacggcttgc acaggtttcc acctcgaccg
     7261 tttctcacgt tatcaacaat agccgatttg tcagcgagga aattcgggaa agagtgcaga
     7321 gggtggtgaa agaactgaat tacacgcctt ctgcattggc tcgtagcctc aaagtgaatg
     7381 aaactaaaac catcggaatg ctggtaacgg caaccggcaa tccgtttttt gcggaggtga
     7441 tggcgggggt ggagcaatat tgccagcaac atcaatataa cctgattatt gccactacca
     7501 acggtgacac cgagcgtttg caacaccatt tgcaaatgct gatgcaacga aaagtagatg
     7561 gcttgttact gatgtgtggc gataccagat tgaatgccga tgagagcttg aatattccgc
     7621 tgccattggt ggtgttagac tggtggttta ccgagctcaa tgcagataaa atctttgaaa
     7681 attcaatatc cggcggctat ctcgcaacaa aaaccttaat tgaagcaggg cattctaaga
     7741 ttgcgattat tactggaaac ctaaaaaaat cattggcaaa aaatcgttta gaaggctaca
     7801 aaaaagcact tgccgaggcg aatattccac tcaatccaaa ctgggtgttc gaaagccatt
     7861 ttgattttgc cggcggggtt aatggaatga atgctttgct tgcccaaagc gaaaagccga
     7921 cagcggtatt tgcctgtagc gacaccattg ctctaggggc ttaccaagca atctggcaac
     7981 aaggtttacg aataccgcaa gatatttcga ttatcggcta tgacgatatt gaactggcaa
     8041 aatacctttc cccaccgctg agtaccatta gccagcccaa agcggaactc gggcggcttg
     8101 cggtggaaac tttgctaaaa cgaattcgtt ttaaatccac tgattttcaa accataatgc
     8161 ttgaacctga attagtttta aggcattcaa tccaaacaat aaacaaaaac gctgaataat
     8221 gtgaagttat tcagcgtttt ttatccgatt tcaattagcc tttttgctgt tccaaataaa
     8281 gcacggtggc ggcaactcgg gaatgcacgt ttaatttacg cagtaaattg cggatatgca
     8341 cttttacggt ttcttcggaa ataaagagct gagccgcaat ttgtttgttc gacataccgg
     8401 ttgcgagcca ttgtaaaaca tccagttcac gatctgttaa gcaacttaaa ggatcatttt
     8461 ctggctggcg gttgagtaaa tgttggcgaa cggtttcgct taataccact tcaccggcag
     8521 ccgctttacg gatattatct agtagttccg gggtgtcggt atcttttaat aaatagccgt
     8581 ccacaccagc atccattaat gcaaatacat cggattgctc gtctgacact gttaaaacaa
     8641 tgattctggc atctatacct tgagcgcgca atgagcgtaa agtatctagc cctgaaagcc
     8701 ctttcatatt taaatccaga ataatcacat ccggctcaag tcgtaatgca agtttgatac
     8761 cgtcggtgcc gttgcttgct tcacccacta cactaaattg gtgatctaat tccaatagtt
     8821 gacgcattcc ctgacgcatt aatgggtggt cgtcaatgag taaaatttga gttggagctg
     8881 tcatttattt ctcctttcct ttctcttaat cggttaattt tgcaattatt aattcaaaat
     8941 agcaagttta gggatttact aaatgccgct tttttgatct tagctaattt ttaactaaaa
     9001 aagaaattgc aaaactttgc aagaaaaaga ccgcttgttc ggctataatt actgccaatt
     9061 tttatttcaa gtgtgaaaag aatgacaaat caaacaacct attttgccac ggcagcccgt
     9121 ggttttgagg aaatactaaa aaccgaactg gagcagattt gtgcggcaga atgcaaagtc
     9181 gctcaaggcg gtgtgcattt taccaccacg caaaaagggg cttatcaggc attattacac
     9241 agccgtttag cctctcggat cttactgccg ctcatcagca ccaaaatttt tagtgatagc
     9301 gatttatatg cttccatcgt gtcatttaac tgggcggatt tattcgatcc ccgtgatacc
     9361 ttttacattg atttcagcgg cactaaccga gaaatccgca acacccaatt tggggcaatg
     9421 cgagtgaaag acggcattgt ggattacttt gaacgcaaag gttttgcccg cccaacagta
     9481 gataaagata atccggatgt tcgcattcat gtttatttgg atcgtgaaaa tgttgtggtt
     9541 tcgcttgatt taagtggaga tgccttacat attcggggct atcgggaaga aaccggtaaa
     9601 gctccgttgc gtgaaacatt ggcggcagcc attattttgc gttccggctg gaaagtcggc
     9661 acgccattgg ttgatccgat gtgtggttca ggcacgttac tgattgaagc agcacaaatg
     9721 gcagcgaata ttgcccctca acttcaccgt aaacattggg gctttaatgc gtggaaaggg
     9781 catcaacaag cgatgtggaa agccgtgtta gatgaggcat tccgaaatgt tgaattgggt
     9841 gcggtagggg agaattgcaa ttcgtcccta caaaaaatgt tcttcggttt tgatctcgat
     9901 catcgtgtgc tgactaaagc aaagcaaaat gcgaaaaatg caggggtaga tcatctcatt
     9961 caatggcaac aaggtgatgt ggctgcgtta aaaaatccga ttccggaaaa aatcggcacg
    10021 gtggtgtgta acccccctta tggtgaacgt ttaggcacaa cgccggcgtt aattgctctt
    10081 tattcggtgt tcgggcaacg cctaaaacag caatttgccg gttggaatgc ctcaattttt
    10141 agcggcgagc ctgaattgtt aaattgcctg cgtttacgtt cagcccgtca attcaaagcg
    10201 aaaaacggtc cgttggattg tgtgcagaaa aattactcca tcagcgagca taacaagcgg
    10261 tcagatttag atgaaaattt acaatttgaa cagaatgctc aagtcgctcc ggattttgca
    10321 aatcgcttaa ccaaaaatat caaaaagatc gaaaaatggg cgaaacaaca aggcattgat
    10381 gcctaccgtt tatacgatgc cgatctgcct gaatataatt tagcggtgga cagatatggc
    10441 gaccacattg ttgtgcagga ataccaagcc ccgaaaaata ttgatgagca aaaagcccgc
    10501 caacgcttgt tagatgcagt ctctgctacg ctttatgtga caggtgttga aactaataaa
    10561 ttagtgttga aagttcgcca aaaacaaaaa ggcacgaatc agtacgaaaa acttgccaat
    10621 aaaggcgagt atttctatgt gaacgagtat ggggcgaaac tgtgggtgaa tttaaccgat
    10681 tacttggata cgggtttgtt cctagaccat cgcttaaccc gtaaaatggt gggcgaaatg
    10741 gcaaaaggca aaacattcct gaatctgttt gcctacacgg gttcagctac ggttcacgct
    10801 gctcttaatg gggcaaaatc gaccactaca gtggatatgt cgaacaccta tttgaactgg
    10861 gcggagcaaa atttagagct gaataattta tcacttcgcc atcatcgttt gttccaagcc
    10921 gattgcttgc aatggctggc agaatgccgt gagcgatttg agctgatttt tgtcgatccg
    10981 ccgactttct ccaactcaaa acgaatggaa aacagttggg acgtgcaacg agaccatatt
    11041 gagttaatga aacagcttaa acgtatttta accgctgatg gtaccattgt gttctcgaac
    11101 aataagcgtg gctttaaaat ggattttgac ggtttggcag cattaggctt aacggcggaa
    11161 aatatttcgc ataaaacctt accgttggat tttgaacgca atccgcaaat ccataattgt
    11221 tggattgtga agaatgtata gataataccc tcg
//
LOCUS       DANKBV010000069        10654 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.069, whole genome shotgun sequence.
ACCESSION   DANKBV010000069 DANKBV010000000
VERSION     DANKBV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10654
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.069"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(145..492)
                     /gene="frdD"
                     /locus_tag="PWY43_001523"
     CDS             complement(145..492)
                     /gene="frdD"
                     /locus_tag="PWY43_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDL2136393.1"
                     /translation="MSLDQNPKRSNEPPVWLLFSAGGMVSALFFPVVIFIIGLLLPFG
                     LVSPDNIISFAHTWIGKLAIMVLAIFPMWAGMHRIHHGLHDLKVHTPAGNVIFYGLSI
                     LYTILVIIAVVQI"
     gene            complement(506..898)
                     /gene="frdC"
                     /locus_tag="PWY43_001524"
     CDS             complement(506..898)
                     /gene="frdC"
                     /locus_tag="PWY43_001524"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDL2136394.1"
                     /translation="MSATKRRPYVRGMKASWWKKLDFYKMYMVREATCLPTVWFCIVL
                     FYGAVALSKGTFATDFVDFLQNPLVVILNIISLAAMLYHAATLFVMTPAVMSVMVKGK
                     HLPVSVGRNILWAATGVISVIALILVYI"
     gene            complement(908..1639)
                     /locus_tag="PWY43_001525"
     CDS             complement(908..1639)
                     /locus_tag="PWY43_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HDL2136395.1"
                     /translation="MANLDKMTIEVLRYNPESDSEPHLDRYEVPYDSQTSLLDALGFI
                     KDELEPELSYRWSCRMAICGSCGMMVNGKPKLACKTFLRDYSGFMRIEPLANFPIERD
                     LVVDLSHFIDSIEAIKPYIIDNKAPEGQRTKQTPAQLEKYRQFSMCINCGLCYAACPQ
                     FGLNPEFIGPAAITLAHRYNLDNRDNGREERMKLLSSKNGVWSCTFVGYCSEVCPKHV
                     GPASAVNQGKLESAKDYVISMIKPR"
     gene            complement(1655..3457)
                     /gene="frdA"
                     /locus_tag="PWY43_001526"
     CDS             complement(1655..3457)
                     /gene="frdA"
                     /locus_tag="PWY43_001526"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDL2136396.1"
                     /translation="MQSVNFDVAIIGAGGGGLRAAIAAAEANPNLKIALISKVYPMRS
                     HTVAAEGGSAAVIKDTDSFDNHFNDTVGGGDWLCEQDIVEYFVEHSPIEMTQLERWGC
                     PWSRREDGEVNVRRFGGMKIERTWFAADKTGFHILHTLFQTSIKYPNIVRFDEHFVLD
                     ILTDNGEARGCVAMNMMEGTLVQINANAVVIATGGGCRTYRFNTNGGIVTGDGLSMVY
                     RHGVALRDMEFVQYHPTGLPNTGILMTEGCRGEGGILVNKDGYRYLQDYGLGPETPIG
                     KPENKYMELGPRDKVSQAFWQEWRKGNTLKTAKGVDVVHLDLRHLGEKYLLERLPFIC
                     ELAKAYEGVDPAKAPIPVRPVVHYTMGGIEVDMNAETSIKGLFAVGECASSGLHGANR
                     LGSNSLAELVVFGKVAGENAARRALEVTARNQPQIDAQAKDVVARLHALANQEGNESW
                     SDIRNQMGDAMEEGCGIYRTQESMEATVNKIQELKERYKNISVKDKSSVFNTDLLYKI
                     ELGFILDVAQSIACSAVERKESRGAHQRLDYVERDDVNYLKHTQAYYNAEGTPTIKYS
                     DVKITKSQPAKRVYGAEAEAQEKVKKAAEAQAQQ"
     gene            3789..4772
                     /gene="epmA"
                     /locus_tag="PWY43_001527"
     CDS             3789..4772
                     /gene="epmA"
                     /locus_tag="PWY43_001527"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDL2136397.1"
                     /translation="MNNLQLDNIDWQPTASIPHLIQRSKIITEIRKFFTDRGLLEVET
                     PILSEFSVTDVHLSTFNTQFQSPFSPEAKTLHLMTSPEYHMKRLLAAGSGPIFQLCKV
                     FRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESFSYQFVFQTY
                     VGLDPLSATKAQLVEKARKHGLQCDDDEQRDTLLQFLFSEVVEANIGQERPTAVYHFP
                     STQAALAQISSEDHRVAERFEFYYKGLELCNGFHELDDADEQIHRFEQDNLQREKMGL
                     EPQQLDVRFLAALKAGFPNCSGVALGVDRLLMLAMDAEKIEEVISFGIEKA"
     gene            4854..5744
                     /gene="rarD"
                     /locus_tag="PWY43_001528"
     CDS             4854..5744
                     /gene="rarD"
                     /locus_tag="PWY43_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL2136398.1"
                     /translation="MNISGKLQGWHYALLCYLTWGTFPIYWYPINQSGMPAEQILAQR
                     VLWAALFAVFLLLIFQQGRAVINAFKQPKVLGLFVLSSFMIGVNWLVYLWAIVNEHIL
                     DASLGYFINPIFNVFVGRLILKEQLNKAQLLALLFAISGILWLAIPAGQIPWVALILA
                     ASFCIYGLIRKLAPMEPLSGLALETLLMSPFAITYLCFCYHQNILVFSELNPLQMAVL
                     LCSGIATTLPLLWFAMGARRISMSLLGMLQYISPTLQFLCGVLIFNETLSAERAVGYI
                     LVWIGVGVFLLGMKKAGVKR"
     gene            complement(5755..6366)
                     /locus_tag="PWY43_001529"
     CDS             complement(5755..6366)
                     /locus_tag="PWY43_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2136399.1"
                     /translation="MKYEAKPLIISPNHIQQRLHGKLLARALLIIALLMLAKFIYIAS
                     LSNYFTFYLMEKFQLNIAMAQLSLFTFLGVVALGTFVGGPIGDKIGRNAVIWFSFLGM
                     APFALLLLHADLFWTIVCAIFAGFIMSSAFSAMVVYAQEAVSGRVGLISGTMFGLMFG
                     IGGLSAAVLGFLADEYGLESIFKACAYLPLLGFAALGLPKRNI"
     gene            complement(6394..6933)
                     /locus_tag="PWY43_001530"
     CDS             complement(6394..6933)
                     /locus_tag="PWY43_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2136400.1"
                     /translation="MHTQHNAQKMVLPILLLISLSHLLNDLVQAVMVSIYPMLKENYQ
                     LSFAQIGMISLIYQITASIIQPGIGLYTDKYPKPNLLPVSMLITLLSVILLAFSLNFG
                     VLLIAAALMGIGSAIFHPEASRVARMASGGKFGTAQSLFQVGGNSGSALGPLLVALFI
                     VPLGQSAVIWVVAFVLLAL"
     gene            7125..9134
                     /locus_tag="PWY43_001531"
     CDS             7125..9134
                     /locus_tag="PWY43_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL2136401.1"
                     /translation="MDLIKKLNTGSTFRIPIFLPSLLFVSFVAVFCIIFPQQAQTSLD
                     TIKNSLFQHFSWFYIFAGSIFFLFLIFLSFSRLGDIKLGADTDEPEFGFGSWIAMLFA
                     AGMGIGLMYFGVAEPILHYLKPVQQNLTEPERMKEAMMTTFYHWGIHAWAIYGVIALA
                     LAYFGFRYKLALTIRSGFYPLLKHRISGFWGHLIDIIALCSTIFGLTTTLGFGVMQVS
                     AGFNNLGLIEQSNFTVLAIIVTVAMALAVLSAVSGVGKGVKILSEINLTLAGLLLIFV
                     IITGPTLLLFSSFTENLGYYFSSLLEMSFRTFAYEPEHQGWLSGWTVLYWAWWASWAP
                     FVGLFIAKISKGRTIREFILGVLFVPSLFNILWMTSFGSSAIWFEQQTAGALAEVSGN
                     TEQLLFTFFEQLPFGSIASFVAVIVISIFFITSADSGIFVLNSIASQGEENAPKWQSV
                     LWGALLAILALSLLYSGGLASLQTMTLIIALPFTFIMLILCIGLWKGLMVDNQYFNKK
                     FSQGSQHWAGKDWKQRLEKIINPSNKQDVRHFFIKVARPAFLELIEEFESYGLIAKMN
                     FTNEQNPKLEFEVVKENLRNFIYGIESVPRELSDLVVGDDNLPNIEQNTIYEPITYFL
                     DGRKGYDVQYMTKEELIADVLQQYERFINLAMDNSHDLMTADFNH"
     gene            complement(9199..9666)
                     /locus_tag="PWY43_001532"
     CDS             complement(9199..9666)
                     /locus_tag="PWY43_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDL2136402.1"
                     /translation="MKSLKLKIPPPAWFLLCAALMWLVAQYVPVSLPNYQHTAIFFSG
                     LAVAIIGGAVAVSALWVIHQAKTTYSPFRPQHTAKLVTWGIYRKSRNPMYLSLLLGLI
                     AWALWLGAMLAWLVLPLFVWLITYFQIKPEEQILAQKFGTHYAKYCNQVRRWL"
     gene            complement(9668..>10654)
                     /gene="murJ"
                     /locus_tag="PWY43_001533"
     CDS             complement(9668..>10654)
                     /gene="murJ"
                     /locus_tag="PWY43_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDL2136403.1"
                     /translation="ALAWGVFLGGLLQFLFQIPFMKKEGLLVKPKWAWKDEGVTKVRK
                     LMIPALFGVSVTQLNLLLNQVIASFLITGSISWMYYADRLIEFPLGLFGIAISTVVLP
                     SLSRIAKNKELTEIQRGEHFQNTMDWGVRMVLLLGIPAMIGMAVLAQPIIMTMFMRGK
                     FGFEDVLATSYPLWVMCLGLNSYMLISVLANGFYANQNTKTPVKVGIIAALSNICFGL
                     AFAPFLGYIGLALASACSALVNVSLLYVNLSKNGYYKVSHKTVLFVLKLFIAACVMGA
                     LVAYFTPEIELWALMTMWQKVYWLIWLMILAGGSYFASILVLGIRKKDFRSA"
BASE COUNT         3098 a         2379 c         2071 g         3106 t
ORIGIN      
        1 atcttttttg cagatattta atacaaaacg atcgttttat cggcaaaatt tctaatattt
       61 gcaaattttc ttataaattc gaccgcttgt tagcatatta aaaatgaaaa gaccatctgc
      121 tttatacaag atggtctttc tcatttaaat ctgcacgact gcgatgatta ctagaattgt
      181 gtataagata gacaatccgt agaaaatcac attacctgct ggcgtgtgaa ctttcaggtc
      241 gtgtaatcca tgatggatac ggtgcattcc tgcccacata gggaaaatcg ctaataccat
      301 aatagcgagc ttgccgatcc aagtatgggc aaaggaaatg atattatctg gtgatactaa
      361 cccgaatggc agtaataaac cgataataaa gattaccaca gggaaaaata atgcacttac
      421 cattccccca gcactaaaaa gcaaccatac cggcggttca tttgaacgtt ttgggttttg
      481 atcaagactc attttcttcc ccttcttaaa tataaaccaa gattaatgca attacgctaa
      541 ttacgcctgt tgctgcccaa agaatatttc ttccaaccga gactggtaag tgtttacctt
      601 taaccatcac ggacatgacg gcaggtgtca tcacaaaaag tgttgccgca tgatataaca
      661 ttgctgccaa tgaaattatg tttaggataa caactaacgg attttgtaag aaatccacaa
      721 aatcggtagc aaatgttcct ttacttaacg caactgctcc ataaaataat acaatgcaga
      781 accaaacagt tggaagacag gtagcttcac gcaccatata cattttatag aagtcgagtt
      841 ttttccacca gctggctttc attcccctta cataaggtct acgttttgtt gctgacattt
      901 cttcctccta tctcggtttg atcattgaga taacataatc tttcgcactt tcaagtttac
      961 cttggtttac agccgaagca gggccgacgt gtttcggaca gacttccgag cagtaaccca
     1021 cgaaagtaca gctccaaaca ccgtttttgc tgctgagtag cttcatacgc tcttcacgac
     1081 cattatcacg gttatccaag ttatagcggt gagccaatgt aatcgcagca ggtccgataa
     1141 attcagggtt caaaccaaat tgtgggcaag ccgcataaca taaaccgcag ttgatacaca
     1201 ttgagaattg acgatatttc tctaattgtg ccggcgtttg tttagtacgc tgtccttccg
     1261 gtgctttatt atcaataata taaggtttaa ttgcctcgat gctatcaata aagtggctta
     1321 aatccactac taagtcacgc tcaatcggga agttcgccag cggctcaatt cgcataaaac
     1381 cgctgtaatc acgcaggaat gttttacaag ctaacttcgg cttaccgttt accatcattc
     1441 cgcaagaacc acaaatcgcc atacggcaag accagcgata agaaagttcc ggttctaatt
     1501 cgtctttaat gaagcccaat gcatctaaaa gtgaagtttg gctgtcatac ggcacttcat
     1561 atctgtctaa atgtggctcg ctatctgatt cagggttgta gcgtagcact tcgatggtca
     1621 ttttatctaa atttgccatt ttactactcc ttctctattg ttgtgcttga gcttctgctg
     1681 cttttttgac tttttcttgg gcttcagcct ctgcaccata cacacgttta gccggttgtg
     1741 atttagtgat tttcacatcg ctgtatttaa tcgtcggtgt accctctgca ttgtagtacg
     1801 cttgtgtgtg ttttaagtag ttcacatcat cacgctcaac atagtctaaa cgttggtgag
     1861 ccccacgaga ttctttacgc tctaccgcag agcaagcaat ggattgagcc acatctaaaa
     1921 tgaagcctaa ttcaattttg tagagtaaat cagtgttaaa cacacttgat ttatctttta
     1981 cgctgatgtt tttataacgc tcttttagct cttggatttt gtttacagtc gcttccatac
     2041 tttcttgggt acggtaaata ccacaacctt cttccatcgc atcacccatt tggttacgga
     2101 tatccgacca agattcatta ccctcttggt tcgctaatgc gtgcaagcga gccaccacat
     2161 cttttgcttg tgcatcaatt tgtggctggt tgcgagcagt cacttctaac gcacggcgag
     2221 ccgcattttc accggcaact ttaccgaaca ccacaagctc tgctaatgag ttagaaccca
     2281 aacggttcgc accgtgcaag cctgatgaag cacattcacc tactgcgaat aagcctttaa
     2341 ttgaggtttc cgcattcata tccacttcaa taccacccat cgtgtagtga accaccgggc
     2401 gaacaggaat cggcgcttta gccggatcga ccccttcata ggctttcgct aattcacaaa
     2461 taaacggtaa acgctcaagt aaatattttt cacctaaatg gcgtaaatct aagtgaacta
     2521 cgtccacgcc ttttgcggtt tttaaggtgt tccctttacg ccattcttgc cagaatgctt
     2581 gtgaaacctt atcacgaggg cctaattcca tatatttgtt ttccggtttg ccgattggtg
     2641 tttccggtcc taaaccataa tcttgcaggt aacggtagcc atctttatta actaaaatac
     2701 caccttcacc acgacaacct tctgtcatca aaataccggt atttggcaaa cctgttgggt
     2761 gatattgtac gaactccata tcacgcaaag ccacaccgtg acgatacacc attgataaac
     2821 cgtcgccggt tacaataccg ccgttagtgt taaagcgata agtacggcag ccgccaccgg
     2881 ttgcaatcac aactgcattt gcattgattt gaacaagtgt gccttccatc atattcattg
     2941 ccacacaacc acgagcttca ccgttgtcgg tgagaatatc cagcacaaaa tgctcatcaa
     3001 aacgcacaat attcggatat ttaatcgaag tttggaacaa agtatgcaaa atgtggaaac
     3061 cggttttatc cgccgcaaac caagtacgct caattttcat tcctccaaaa cggcgaacat
     3121 tcacttcacc gtcttcacga cgactccaag ggcaacccca gcgttctaat tgtgtcattt
     3181 caatcggcga atgctctaca aaatactcca caatatcttg ctcacataac cagtcaccac
     3241 caccgacggt atcattaaag tgattgtcga aagagtcggt atctttaatt accgccgctg
     3301 aaccaccttc tgctgcaacc gtatggctac gcattgggta aacttttgaa attaaagcaa
     3361 tttttagatt tggatttgct tcagccgccg caatagcagc acgcaagcca ccaccaccgg
     3421 cacctataat cgcgacatca aaattaacac tttgcataat gcactccgaa agttaaataa
     3481 cttaattcat taaaaagtaa tcatattgtg ccactatttg agcaatgaga taacctattt
     3541 ttacaaattt gtaacataaa aaatgtaatt ttgtgatcta cctcaaaaaa ggtaataaag
     3601 cttataaagg tattgagaat ggctcttaat tggacagtga tagaaagaaa atttctaatg
     3661 tttaaaatga gaaaatttaa tttgttgcaa aaaagttaaa accctctctt tatttttctt
     3721 gatcggtaac tagggcaact cactttttta ggctaaaata gcctcacatt ttcaagcggt
     3781 cattttttat gaacaattta caactcgata atattgattg gcaaccaaca gcctctattc
     3841 ctcacctgat ccaacgttca aaaatcatta cggaaatccg caagttcttt accgatcgag
     3901 ggcttttaga ggtagaaacc ccgattttaa gcgaattttc ggtgacagac gttcatcttt
     3961 cgactttcaa tacgcagttt caatcgcctt tttcccctga ggcgaaaaca ctccatctga
     4021 tgaccagccc ggaatatcat atgaagcgac tacttgctgc tgggagtgga ccgattttcc
     4081 aactgtgtaa agtatttcgc aacgaggaag ccggtaagcg acacaaccct gaatttacga
     4141 tgctggagtg gtatcgcccg cattttgata tgtatcgctt aatcaatgaa gtggatgatt
     4201 tactgcaaca aattttggac tgcgaaccgg cagaatcatt cagctatcaa tttgtgttcc
     4261 aaacctatgt cggattagat ccgctttctg ccaccaaagc acaacttgtg gaaaaagccc
     4321 gaaaacacgg cttgcaatgt gatgatgatg aacagcgtga caccttgtta caatttttat
     4381 tcagcgaggt tgtcgaagcc aatatcgggc aagaaagacc aactgccgtt tatcatttcc
     4441 cttccaccca agcggcatta gctcaaatta gctcggaaga tcaccgtgtt gccgaacgtt
     4501 ttgagtttta ctacaaaggc ttggaactct gtaacggctt ccacgaatta gacgatgctg
     4561 atgagcaaat tcatcgcttc gagcaagata atctgcaacg tgaaaaaatg gggctggagc
     4621 cacagcaact agatgtgcgt tttcttgccg cactcaaagc gggttttcca aactgctcag
     4681 gtgtcgccct tggggttgat aggctgttaa tgctggcaat ggatgcggaa aaaattgaag
     4741 aggttatttc tttcggcatt gaaaaagcct aattttttat gccgttttgt gatttgcaat
     4801 ttgcaaaaaa atcgacaaat atgaccgctt gtacactcgt ttaggaaagc aaaatgaata
     4861 tttcaggcaa attacagggt tggcactatg ccctactgtg ctacttaact tggggaacct
     4921 ttccgattta ttggtatccg atcaaccaat cgggaatgcc tgccgagcaa attttggctc
     4981 aacgggtgct ttgggctgcc ctatttgctg tgtttctgct gctgattttc caacaagggc
     5041 gagcggttat taatgccttt aaacagccaa aggtactcgg gctgttcgtg ctttcttctt
     5101 ttatgattgg cgtgaactgg ctggtttact tatgggcaat cgtcaatgag catattttag
     5161 atgccagtct aggttatttt atcaacccca ttttcaacgt gtttgtcggt aggcttattc
     5221 taaaagagca actgaataaa gcccaattat tggctctgct gtttgcaatt agcggcattt
     5281 tatggcttgc tattcctgcc ggacaaattc cgtgggtcgc attgattcta gccgccagtt
     5341 tctgtattta cgggctgatt cgtaaacttg ccccaatgga acctttgtct ggtttggcgt
     5401 tagaaacttt gctgatgtcg ccttttgcca tcacctatct ctgcttttgt taccaccaaa
     5461 acattttggt attcagtgaa ctcaatcctt tacaaatggc agtgctactc tgctccggca
     5521 ttgccactac cttaccattg ctatggtttg caatgggtgc aagacgcatt tcaatgtcgc
     5581 tactcggtat gctgcaatat atttcaccta ccctgcaatt tttatgcggt gtgctgattt
     5641 ttaatgagac actttccgcc gaacgtgctg taggctacat tctggtttgg attggggtgg
     5701 gggttttctt attgggtatg aaaaaagctg gtgtcaaacg ctaaaaataa aacgttaaat
     5761 atttcgtttt ggtagcccta atgcagcaaa accaagtagt ggcaaatagg cacaggcttt
     5821 aaaaatactc tccaagccat attcatccgc caaaaaaccc aatacggcgg cacttaagcc
     5881 gccaatacca aacattaaac cgaacattgt gccggaaatc agccctactc tgcctgatac
     5941 ggcttcttga gcataaacca ccatagcgga gaacgctgat gacataataa aaccggcaaa
     6001 aatcgcacac acaatcgtcc aaaagagatc tgcgtgaagc aacaacaaag caaaaggtgc
     6061 catacctaag aaagagaacc aaatcaccgc atttcgtcca atcttatcgc caatcggccc
     6121 gccgacaaaa gtgcctaaag caactacacc taaaaaggta aatagagaga gctgtgccat
     6181 tgctatattg agttggaatt tttccattaa ataaaaggtg aaatagttac tgaggctcgc
     6241 aatataaata aatttcgcta acatcaataa agcgataatt aataatgctc tggctaataa
     6301 tttaccgtgt aagcgttgtt gaatatggtt tggtgaaata atgagcggtt tggcttcata
     6361 tttcacccaa cggcttactt tatataaaac ccatcataat gccaataata caaatgcaac
     6421 aacccaaatc accgcacttt gccctaacgg cacaataaat aatgcaacta agagcggacc
     6481 taacgccgat ccactgtttc cacctacttg gaaaagcgac tgagctgtac caaatttacc
     6541 gcccgatgcc attcgagcca cccgagaggc ttcagggtga aaaatggcag agcctatccc
     6601 cattaacgca gcggctatca gtaacacgcc aaaattcagc gaaaaggcaa gtaaaatcac
     6661 ggaaagaagc gtaatcagca tactgacggg caataaattc ggttttgggt atttatcggt
     6721 ataaagccca atacccggct gaataataga tgcggtaatt tgataaatga gtgaaatcat
     6781 accaatttga gcaaaactca gttggtaatt ttcttttagc attggataga tcgaaaccat
     6841 tactgcttga actaaatcat tcaacaaatg agaaagggaa atcagcagta aaatgggcaa
     6901 aaccattttt tgagcgttat gttgtgtgtg cattctttat tcgtttttta taaatgaaaa
     6961 atattctact cgcaaataat gaatgtgcaa gcggtcaaat ttcccgattt ttttaccaaa
     7021 cccattaggt tgcaatatta ttaccaatac gtcttaaatt tgttattttt atatcttttt
     7081 gctatattca agcctctaac cattcaaaaa tagaggaaat agcattggat ttaatcaaaa
     7141 aattaaacac aggaagtacc tttaggatac cgattttcct accgagttta ctctttgtca
     7201 gctttgttgc cgttttctgt atcatctttc cacagcaagc acaaacctca cttgatacca
     7261 tcaaaaatag tctcttccaa cattttagct ggttctatat ttttgcaggc tctatctttt
     7321 tcctgtttct aatttttctc tctttcagcc gattgggtga tattaaatta ggggcagata
     7381 ccgatgagcc tgaatttggt tttggctctt ggattgcgat gttattcgcc gccggaatgg
     7441 ggatcgggtt aatgtatttt ggggtagcag aacctatttt gcattacctt aaacccgtcc
     7501 aacaaaattt aactgagccg gagcgtatga aagaagcgat gatgacaacg ttctatcatt
     7561 ggggtattca cgcttgggca atttatggtg tgattgcctt agctcttgct tattttggtt
     7621 tcagatataa gttagcactc actattcgtt ccggatttta tcccttacta aaacatcgta
     7681 tttcaggctt ctgggggcat ttaattgata ttattgccct ttgtagcacg attttcggtt
     7741 taacgactac acttggcttt ggggtgatgc aggtcagtgc tggctttaac aatctaggtt
     7801 taattgaaca gagcaatttt actgttcttg cgattatcgt aacagtagca atggctcttg
     7861 ccgtgttatc tgccgtttcg ggcgtaggca aaggggttaa aatcttaagt gaaatcaatc
     7921 tcacattagc cggattgcta cttatttttg tgataatcac cggcccaact ctattacttt
     7981 tctcaagctt caccgaaaat ttaggctatt attttagctc gctgcttgag atgagtttcc
     8041 gtaccttcgc ttatgaaccg gaacatcaag gctggctaag cggctggacg gtcctttatt
     8101 gggcatggtg ggcatcttgg gcgccatttg ttggtttgtt tattgccaag atctctaaag
     8161 gcagaaccat tcgtgaattt attttagggg tgctatttgt tccatcgctg tttaacattt
     8221 tatggatgac cagcttcggc agctctgcca tttggttcga acaacaaact gccggtgctt
     8281 tagctgaagt cagcggcaat accgaacaac tgttatttac cttttttgag caattaccgt
     8341 ttggctctat tgcctctttc gttgccgtca ttgttatcag tattttcttt atcacctctg
     8401 ccgactcggg gatttttgtt ctcaacagca ttgcttcaca aggcgaagaa aatgcaccga
     8461 aatggcaaag cgtgctttgg ggagcattat tagccatctt agcgttatca ctactctatt
     8521 cgggtggctt ggcttctctg caaacaatga cactgattat cgccttacca tttaccttca
     8581 ttatgctgat tctctgtatc ggcttatgga aaggattaat ggtagataac caatacttca
     8641 acaaaaaatt ctcgcaaggt agccaacatt gggcgggtaa agattggaaa caacgcttgg
     8701 agaaaatcat caacccaagc aataagcaag atgtccgtca cttctttatt aaagttgcca
     8761 gaccggcatt tttagaactt atcgaggaat ttgaaagcta tggcttaatc gctaaaatga
     8821 atttcaccaa cgaacaaaac ccgaaattag agtttgaagt ggtgaaagaa aatttacgca
     8881 atttcattta cggcattgaa agtgtgccac gggaattatc ggatttggtg gtaggtgacg
     8941 acaacctacc gaacattgag caaaatacca tttacgagcc gattacttat ttcttagacg
     9001 ggcggaaagg ttatgatgtg caatatatga ccaaagaaga gttgattgcc gacgtgctgc
     9061 aacagtatga acgctttatc aatttagcga tggacaactc gcacgactta atgacggctg
     9121 atttcaatca ctaaccccac aagcggtgag attttcggaa aaatttgcaa atttccaaca
     9181 aaatctcacc gcttgcattt aaagccaacg ccttacttgg ttgcaatatt tggcataatg
     9241 tgtgccgaat ttttgggcta aaatttgctc ttccggcttg atttggaaat aggttatcaa
     9301 ccatacaaac agcggtaaaa ccagccacgc caacattgcc cccaaccata atgcccacgc
     9361 aatcaaacct aacagtaacg aaagatacat tgggtttcgg cttttgcggt aaatgcccca
     9421 agtgaccaat ttagcggtat gttgtggtcg aaaaggactg tatgtcgttt tcgcttggtg
     9481 tatgacccaa agtgccgaga cggcaaccgc tccgccaatt atcgccacag ctaaaccgct
     9541 aaaaaatatg gctgtatgct gataattcgg caagctcacc ggcacatatt gagctaccag
     9601 ccacattaaa gcggcacaaa gcaagaacca agccggcggc gggattttca gttttaatga
     9661 tttcatttta tgccgagcgg aaatcttttt tacgaatgcc taataccaaa atagaggcaa
     9721 aataactacc gccggctaag atcatcagcc aaatcagcca atacactttc tgccacattg
     9781 tcattaatgc ccaaagttca atctcaggcg tgaaataggc aaccaacgcc cccattacac
     9841 aagcggcgat gaatagtttc aacacaaaaa gcacggtttt atggcttact ttatagtaac
     9901 cattttttga gagattcaca tacagcaagc tcacattcac taatgccgaa cacgcagaag
     9961 ctaacgctaa accaatgtat cccaaaaacg gagcaaacgc caagccaaag caaatattac
    10021 ttaaggcggc aataatccca acttttaccg gtgttttggt gttttggttg gcataaaaac
    10081 cgtttgccag cacgctaatc aacatatagc tgtttaaccc caagcacatt acccaaagtg
    10141 gataagaggt tgccagcaca tcttcaaaac caaatttacc ccgcataaac attgtcataa
    10201 taatcggctg agccagcact gccataccga tcattgccgg aatgccgagc aacagcacca
    10261 ttctcacccc ccaatccatt gtgttttgaa agtgttctcc acgttggatt tcagtgagtt
    10321 ctttgttttt cgcaatacgg gaaaggcttg gcagcacgac ggtggaaatc gcaattccaa
    10381 ataaaccgag cggaaattca attaagcggt cggcataata catccaacta atcgagccgg
    10441 taatcaaaaa cgaggcaatc acttggttta gcagtaaatt tagctgggta acggaaacgc
    10501 caaataaagc cggaatcatt aatttacgga ctttagtgac accttcgtct ttccacgccc
    10561 attttggctt aaccagtaag ccctcttttt tcataaatgg aatttggaat aaaaattgca
    10621 ataagccgcc taaaaatacc ccccacgcta aggc
//
LOCUS       DANKBV010000070        10645 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.070, whole genome shotgun sequence.
ACCESSION   DANKBV010000070 DANKBV010000000
VERSION     DANKBV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.070"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            72..752
                     /locus_tag="PWY43_001534"
                     /pseudo
     CDS             72..752
                     /locus_tag="PWY43_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(854..1519)
                     /gene="cas4"
                     /locus_tag="PWY43_001535"
     CDS             complement(854..1519)
                     /gene="cas4"
                     /locus_tag="PWY43_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDL2136404.1"
                     /translation="MDDLQNSTEILPLVQPKFGYDKRSISLSALQHYAFCPRQCALIH
                     NEQVWAENYLTAQGQVLHERVDSGEPETRKGIRFERTVHVAAEKLGISGILDLVERDL
                     KTGELKPVEYKRGKPKPEPMDEIQLCAQALCLEEMTGQTINEGALWYMQTRHRLPITF
                     STELRTKTLATISEVRSLLNSGTTPLPKYSKRCKACSLIDLCQPQLLDRDRSERYVEE
                     IFK"
     gene            complement(1519..2382)
                     /gene="cas7c"
                     /locus_tag="PWY43_001536"
     CDS             complement(1519..2382)
                     /gene="cas7c"
                     /locus_tag="PWY43_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HDL2136405.1"
                     /translation="MSIQNRYEFVFFFDVTNGNPNGDPDAGNMPRLDPETSKGLVTDV
                     CLKRKIRNFIEMSYENEAGYEIYVKEKSVLNLQNKRAYEALGIESEAKKLPKEEAKAR
                     EITAWMCKNFFDIRTFGAVMTTEVNSGQVRGPVQLAFAQSIDPIIPLEVSITRMAVTN
                     EKDLEKERTMGRKYIVPYALYRVHGFISAKLAEKTGFSDEDVQKLWQALQLMFEHDRS
                     AARGEMAARKLIVFKHDSELGNQPAHKLFDSVKVERINGEKDTPAKDYDDYRISVQAE
                     GLTGVTVEELL"
     gene            complement(2392..4176)
                     /gene="cas8c"
                     /locus_tag="PWY43_001537"
     CDS             complement(2392..4176)
                     /gene="cas8c"
                     /locus_tag="PWY43_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HDL2136406.1"
                     /translation="MILSALTRYYYRLAAQEDRLTGEAKVPSYGFSEEKIGWVLELDS
                     KGNLVNVISNLTEDKKPKPKLMSVPRPEKRTSGIKPNFLWDKTAYVLGIESNPDKATA
                     KDQPINFAEKTFEAFKQYHLDLLADSNDEGLMAIRNFLQQWQPTHFAESICPREILDA
                     NVVFKLVGTSGYIHQREAAQTLWTSLLINQSDNAEKGLCLISGKLSPIQRTHPPIKGV
                     FGGQSSGGSIISFNKESFTSFGKEQGSNAPVSEQAAFAYTTALNYLLRTEKHRLTIGD
                     ASTVFWAEADNTTQAEAAEGFFAALMTPPDDEQENQKIFEELQKISKGRPLAEISPEL
                     SENTRFYVLGLAPNAARISVRFWLDTTFGQLAKNLSEHWQDLALEPCAWKTPPSIWRL
                     LLQTASLGKSENISPVLAGEMARAVISGHLYPMSLLSQLVTRIRADGDINGLRVALMK
                     AVLQRKFRKGFIKEGVPMSLNKESDNQAYLLGRLFAVLERIQTQALGELNAGIADRYY
                     GSASAVPFSVFPRLLSGAKHHLSRLRKDKGGMAVNLDKDLGEIIASLPENFPRHLSID
                     EQGRFAIGYYHQKQSYFAKKETTESIEN"
     gene            complement(4173..4850)
                     /gene="cas5c"
                     /locus_tag="PWY43_001538"
     CDS             complement(4173..4850)
                     /gene="cas5c"
                     /locus_tag="PWY43_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HDL2136407.1"
                     /translation="MPNKIRLHIWGDYACFTRPEMKVERVSYDVITPSAARGILAAVH
                     WKPAIRWVIDKIYVLQPIQFESVRRNELGSKISASKISGAMKRKTVADLYTVIEDDRQ
                     QRAATVLKNVGYVIEAHAVLTKMAGEAETVTKHIEMFKRRAKKGQCFQQPCMGVREFA
                     ANFALIDDDEPLPECFLAENERDRDLGWMLHDIDFEHGNQPRFFRAEMKNGVIDVPPF
                     YAEEVKS"
     gene            complement(5088..6839)
                     /locus_tag="PWY43_001539"
     CDS             complement(5088..6839)
                     /locus_tag="PWY43_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2136408.1"
                     /translation="MANETLNFKDNARLGRVVTVDTSRVLISVEEHQQMTSIAVGNLI
                     AIQGRTASEYLIGIIERVTRSISEKLLLEEISNEGEIPLGESQDDFIKAVLIGTYKAV
                     DGSLSNTFKRGADCFPQIDRDCFHISGSNLQLFMNLLSANVKENERLTLGHFMADETA
                     VAIANGDKFFQRHAAILGSTGSGKSWSVALILERAAALKAANLIVFDMHGEYSTLCEG
                     KNAIAQQYRIAGPGDLDGSDERSLFIPYWLLNREEMLALILDRSDDNAPNQAARFTEH
                     VRALKEQTLNKEERNDVLETFTVDSPIPYSIELLLARLDKDNSEMVPKEGGSGTKQGP
                     WYSKLSRFMGRMKAKIDDRRCGFMFQPPVECNSYGWLENMLSKLFSASESEKGIKIID
                     FSEVPSDVLPIVTGVLARLLYNIQFWMDKDSRHPLTIICDEAHLYLPVRDSAEAAEKR
                     ALEAFERIAKEGRKYGVSLLVVSQRPSDVSRTILSQCNNFLVLRLTNDQDQSVVKRLL
                     PESMVGITDILPLLDLGEALLLGDSILLPTRIKLAKPHIAPESATRQFWSDWANQEPK
                     PNAVSNAVEALRRQVRS"
     gene            complement(6829..7902)
                     /locus_tag="PWY43_001540"
     CDS             complement(6829..7902)
                     /locus_tag="PWY43_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDL2136409.1"
                     /translation="MEGKRKMREPLLDEDELKKWLQTIFTDNLVIIVGSGLSCAEGLP
                     GMGALADRLKERMPECLDDIDKVTWNTISDCLDSEGLEGALLKHQANETIEAAIIKIT
                     AEYVLSEEQKAINKCIAENQKLKFSYLLPHISASNPKIARVITTNYDRLIEFAAEYEN
                     WGIDSMMVGRYWGKHNPDLSRKLQIRDIRVKGKCPKLVYRDHIKIFKPHGSLDWFMAG
                     DVPMTSCIGKVEEPLIITPGVGKYKKGYGQPFDAHREKANEAIDSASSILCIGYGFND
                     DHLQTHLTGKIKSGVKTLLLTRGLSENARNVVKEAPNCKALIFDGDKAGTRVVSKQEE
                     TFIPNVHWWDVEFFVKEILENGK"
     gene            complement(8015..10303)
                     /locus_tag="PWY43_001541"
     CDS             complement(8015..10303)
                     /locus_tag="PWY43_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDL2136410.1"
                     /translation="MEEHTIQSLYAHSGKLSDHSDWQTLLSHANNVGLLAAEFAAPFA
                     SQEIARCTGLLHDLGKYSERYQKRLHGGERVNHSTAGAKIAVERWGPLGKMMAFCIAG
                     HHAGLANGSGEGKNRSTLTQRLQEQFGYGHKDLPVLDAIWQQELNLPEKLPLPPLKPS
                     KNEPFFSYAFFIRMLYSCLVDADFLDTEAFYANLEKRPLERGNSPNLTALHQQFNQFI
                     AQFRERITIQTPNTQEAQRKAEVNRLRSEILDYAVSQVKQETGLFSLTVPTGGGKTFT
                     SMAFALEHAKQYGLCRIIYVIPFTSIIEQNAAEFRKAFGELGEEAVLEHHSTFDHDSL
                     PDKSSRDKLKLASENWDAPVVVTTAVQFFESLFADRSSRCRKLHNIAGSVIILDEAQM
                     LPLNLLRPIMAAIDELARNYQCSVVLCTATQPAIHQANGFYKGFENVREIAPNPTALF
                     EKLKRTTVKHIGIQTDVELQSKLADNPQMLIIVNNRRHARALYDSAKQLEGAYHLTTL
                     MCAKHRSQTLTEIRLRLKQNLPCRVIATSLIEAGVDVDFPLVMRAEAGLDSVAQAAGR
                     CNREGKRLSVESNVWVFQPEEQWKAPPELGLLSGCMRSTVQVHADDLLNPKAINHYFQ
                     AVYEAKGKELDKPNILKACHDAGQSLNFPFQKIAEDFRMITSHLIPVIIPFDQEAENL
                     IDRLRYADQVGGILRKLQPYTVQVPEKALAELYNAGRVETINETNFGKQFYCLIGMDL
                     YDEVAGLSWENVDFLESEKLVL"
BASE COUNT         3032 a         2371 c         1982 g         3260 t
ORIGIN      
        1 aaattattct atgcttatat atggatattc aatatactaa atctaatgca ttgtaataaa
       61 aaggagctta tatgaaacgt agatttttat ttaaaaatta tggtccagat gatcaaacta
      121 taattcaagc aattcaacat tctgttgaaa tatctcggaa aaataacaat tctaacattc
      181 atattgtttt tcccagaaag tctgactttg aaagtactaa tcttgggaaa ataattgatc
      241 aaatattagg agcaggatca gctaaaaaat taactaaagg tgaatctata ggttccttag
      301 atttgctact tccatcaaga attaattctt caaactcatc tggtacaatc ttggctgttt
      361 actgtacatc tcatgatatg catttgatcg actcaaactc ttctgctgat gacattatat
      421 tcgtatcctg gttgccagat gaagctaatc attgggagaa tacttggcat aatcatggac
      481 ttattatcat gaaaggggaa acttctctta atattccaca aataccggaa gaaattgtac
      541 agtcattgac taatttaact gctattatca atctatctac tgggctatcg cacccatcag
      601 ataaaaagca tgctgaacaa atcttcaaag agctatctca aaagggatat aaagcggatg
      661 aagaacaaat agctagctga gcaatagctc atggatggaa tgctagccat ttaagcgatc
      721 ttaagaaact agttcgtaga tacctttctt aattcccata acaactacat ttttctacta
      781 tttctatact ttaacacttc aattttagat ggcttttagt atttaaaagc catctatttc
      841 ctatgtttat aaattattta aagatttctt ccacatacct ttccgaccta tccctatcca
      901 acaactgcgg ttggcagagg tcaatgagtg agcaggcttt gcagcgtttg ctatatttgg
      961 ggagtggtgt tgttccgctg tttaaaagtg accgcacttc ggaaatggtg gcgagggttt
     1021 tggtgcgtag ttcggtggag aaagtgatcg gcaagcggtg gcgggtttgc atataccaca
     1081 atgctccctc gttaatagtt tgccccgtca tttcttccaa acataacgct tgggcgcaaa
     1141 gttggatttc gtccatcggt tcaggttttg gcttgccacg tttatattcc accggtttta
     1201 gttcgcctgt tttcagatct cgctcgacca aatccaaaat accactgatg cctaattttt
     1261 ccgctgcaac gtgaaccgtt cgctcaaagc gaatgccctt tcgggtttct ggctcaccac
     1321 tatctacccg ctcgtgcaac acttgacctt gtgcggtgag atagttttct gcccacacct
     1381 gttcgttatg aattaacgca cattgacgtg ggcagaaggc gtagtgttgc aaagcggaaa
     1441 gcgaaatcga ccgcttgtca tagccaaatt ttggctgaac aagcggtaaa atttccgtag
     1501 aattttgcaa atcgtccatt acagcaattc ttcaacggtt acacccgtta agccttcagc
     1561 ttgtacgctg atgcggtagt cgtcatagtc tttcgctggc gtgtcttttt caccgttgat
     1621 tcgctcaact tttaccgagt caaacagttt gtgtgcaggt tggttgccta attcgctgtc
     1681 gtgtttaaat acgatcagtt tacgagctgc catttcgcct cgtgcggcag aacggtcgtg
     1741 ttcaaacata agttgcaagg cttgccaaag tttttgtaca tcttcatcag aaaagcccgt
     1801 tttttcagcg agtttagctg aaataaagcc gtgaacacga taaagtgcgt aaggcacgat
     1861 atatttacgc cccatagtcc gttctttttc aagatctttt tcgttggtca ctgccattcg
     1921 tgtgatcgat acttctagcg gaataatcgg atcgatagat tgagcgaacg ccaattgcac
     1981 tgggcctcgc acctgtccgc tgtttacttc ggtagtcatt accgccccaa aggtgcgaat
     2041 atcgaaaaag tttttgcaca tccaagcggt aatttctcgg gctttagctt cctcttttgg
     2101 tagttttttc gcttccgatt caatccctaa tgcttcataa gcacgtttgt tttgtaaatt
     2161 cagaacgctt ttttctttaa catagatttc gtaacccgcc tcgttttcat agctcatttc
     2221 aataaaattg cggattttgc gttttaggca aacatcggtg actaaacctt tactggtttc
     2281 aggatcaagg cgtggcatat ttcctgcatc tggatcacca tttgggttac cgttggttac
     2341 atcaaagaaa aaaacaaatt catagcggtt ttgaatagac atagtgtttc cttagttttc
     2401 aatagactca gtggtttctt ttttagcgaa atagctttgt ttttggtggt aatagccaat
     2461 cgcaaagcgg ccttgctcat cgatacttaa atggcgaggg aaattttccg gcaagctggc
     2521 aataatctca cctaaatcct tatccagatt aactgccatt ccgcccttat cttttctcaa
     2581 gcgagataaa tgatgttttg ctccgcttaa taagcgaggg aataccgaaa atggcacggc
     2641 agaggctgaa ccataataac ggtcggcaat gccggcattt aattcaccta acgcttgggt
     2701 ttggattctt tctaataccg caaataacct gccgagcaag taagcttggt tatcgctttc
     2761 tttatttaag ctcataggaa ctccttcttt aataaaacct tttctaaatt ttcgttgtaa
     2821 gaccgctttc atcaatgcta ctcgcaagcc gttaatatct ccgtctgctc ggattcgagt
     2881 caccagctga gaaagcaagc tcatcgggta taaatgaccg ctaataaccg cccgagccat
     2941 ttcgcccgcc aagacaggag aaatattctc acttttacct aatgaagcag tttgtaataa
     3001 aagtcgccaa atggagggag gcgttttcca tgcacaaggc tcaagggcaa gatcttgcca
     3061 atgctcgcta agattttttg ccagttgccc aaaggtggta tccagccaaa aacgcaccga
     3121 aatgcgagct gcattcggag caagccccaa cacataaaaa cgggtgtttt cagataattc
     3181 tggggaaatt tctgcaagcg gtcgtccttt gctgattttt tgcaattctt caaagatttt
     3241 ttggttttct tgctcatcat ctggaggcgt cattaaagca gcaaaaaagc cttcagcagc
     3301 ttcagcttgg gtggtattgt ctgcttctgc ccaaaagaca gtgctggcat caccaatggt
     3361 taaacgatgt ttctcggtgc gtaacaaata atttaaagca gtagtgtagg caaaggcggc
     3421 ttgttcagaa acaggggcat tactaccttg ttccttgcca aatgaagtga aggattcttt
     3481 attaaaagag atgattgaac caccggaact ttgcccacca aatacccctt taataggagg
     3541 atgagttcgt tgaattggcg ataatttgcc tgaaattaag cataaacctt tttcagcatt
     3601 atcactttga tttatcaaca aacttgtcca aagggtttgt gcagcttctc gctgatgaat
     3661 ataaccgctt gtgccgacta atttaaacac gacattcgca tcaagaattt ctcttgggca
     3721 gattgattcg gcaaaatgtg tgggttgcca ttgttgcagg aaatttctta tcgccattaa
     3781 accttcatca ttggaatctg caagcaaatc caaatggtac tgtttaaatg cctcaaaggt
     3841 tttctcggcg aaattgatgg gttgatcttt ggcggtggct ttatcaggat tactttcaat
     3901 gcctagcaca taagcggttt tatcccacag aaaattgggc ttaatgccag acgtgcgttt
     3961 ttcagggcga ggtacgctca tcaatttcgg ttttggtttc ttatcttctg ttaaatttga
     4021 gatcacattg accaaatttc cctttgaatc taattctagt acccaaccga ttttttcttc
     4081 actgaaacca taagaaggca ctttagcttc gcctgttaag cgatcttctt gtgctgcaag
     4141 gcggtaataa tagcgagtta aggcggagag aatcatgatt tgacctcctc tgcataaaat
     4201 ggtggcacat caatcacgcc atttttcatt tccgcacgga agaaacgagg ttgattaccg
     4261 tgctcaaaat caatgtcgtg caacatccag cctaaatccc gatctcgctc attttctgcc
     4321 aaaaaacact caggtaacgg ttcatcgtca tcaatcaagg caaaatttgc ggcaaattca
     4381 cgcacgccca tacaaggctg ctggaaacat tgcccctttt tcgcacggcg tttaaacatt
     4441 tcaatatgct tggtgacagt ttccgcctcg cctgccattt tggtcagcac tgcgtgagct
     4501 tcgatcacat aaccgacatt tttcagcaca gtagcagcac gttgttggcg gtcgtcttca
     4561 atcacagtat ataaatccgc cactgtttta cgcttcattg caccactgat tttactggca
     4621 gaaattttac tgcctaactc attacggcga acagactcaa attgaatcgg ttgtaataca
     4681 taaattttat caatcaccca gcgaattgcc ggcttccaat gcaccgcagc caaaatcccc
     4741 cgtgccgcag acggcgtaat cacatcataa gacacccgtt ctaccttcat ttcaggacgg
     4801 gtaaaacaag cataatcacc ccaaatatgg agcctgattt tgtttggcat aatagaatgc
     4861 tccttttaat tatgtaacac acacctcccc gaagggaggt gaaatttctt tagcttataa
     4921 ctgctttggt ttcaacccac gcactcggta aaatgcgaaa aattttatgg cttattatat
     4981 taagcggtcg gatttactta gaaagttgca aagttatttg agtatttaac cgcttgtatt
     5041 tcaaaatagt cagaaaaata acagcacttt ctaggtacaa tatttttcta agaacgaact
     5101 tgtctccgca atgcctctac tgcgtttgac acagcatttg gtttgggctc ctggtttgcc
     5161 caatcagacc agaattggcg tgtagcactt tctggggcta tatgcggttt agcaagtttg
     5221 attcgtgtag gtaatagaat cgaatcgcct agcaataacg cttcgcctaa atcaagtaac
     5281 ggcaatatat cagtaattcc aaccatagat tccggcaata aacgctttac aacactttga
     5341 tcttgatcat ttgttaatcg taaaaccaag aaattattac attgactcaa aattgttcgg
     5401 cttacatctg aaggacgttg gctaacgacc aacaaagaca cgccatattt tctaccttct
     5461 ttcgcaatcc gctcaaaggc ttccaatgcg cgtttttccg ctgcttccgc tgaatctcga
     5521 acagggagat ataaatgtgc ttcatcgcaa ataatcgtta gtggatggcg ggaatcttta
     5581 tccatccaaa attgaatgtt gtataaaagg cgggcaagaa ctcccgtaac aatcggtaaa
     5641 acatccgacg ggacttccga aaaatcaata atttttatcc ctttttcgct ctcagaagca
     5701 gaaaacagtt ttgacaacat attttccagc cacccatagc tgttgcactc tactggcggt
     5761 tggaacataa aaccgcaacg tctgtcatct attttggctt tcatccttcc cataaaccta
     5821 gacaacttac tataccaagg accttgcttt gttcctgaac ctccctcttt aggtaccatc
     5881 tcagaattat ctttatctaa acgagctaac aataattcaa tagaatacgg aattggcgaa
     5941 tcaacagtaa acgtttccaa aacatcattt ctttcttcct tattcaatgt ttgctctttt
     6001 aatgcgcgaa catgctcagt aaaacgcgcc gcctgattcg gagcattatc atcactgcga
     6061 tctaaaatta aagccagcat ttcttcccga ttgagcagcc aatagggaat aaataaactg
     6121 cgttcatctg aaccatccaa atctcccggt ccagcaatac ggtattgctg agcgatagcg
     6181 tttttacctt cgcatagagt gctatattca ccgtgcatat cgaatacaat aagattagca
     6241 gcttttaaag ctgctgcacg ttccaatatt aatgcaactg accagctttt accggaacca
     6301 gtactaccca aaatagcagc gtgccgttga aagaatttat ctccgtttgc tatagctaca
     6361 gccgtttcat cagccataaa atgacctaaa gtcagccgtt cattttcttt tacgttagcg
     6421 gacaaaagat tcataaataa ttgcagattg ctgccactaa tatgaaagca atctctatca
     6481 atttgcggaa aacaatccgc tccccgctta aaagtgttgc tcaaactacc atcaacagct
     6541 ttataagtac cgattaatac tgccttgata aagtcatcct gactttcacc cagaggaatt
     6601 tcaccctcat tagatatttc ttctagcagc aatttttcac tgatagaacg cgttacccgt
     6661 tcgataatgc cgataaggta ctcactagcc gttctgcctt gaatcgcaat cagattaccc
     6721 acagcaatac tagtcatttg ttgatgctct tcaacagaaa tcaatacacg gctggtatct
     6781 actgttacaa ccctgcccaa acgggcattg tctttaaaat tcaatgtttc atttgccatt
     6841 ctctaaaatc tcttttacaa aaaactcaac gtcccaccaa tgaacattag gaataaaagt
     6901 ttcttcttgc ttgctaacca cccttgttcc agccttatct ccatcaaaaa tcagagcctt
     6961 acaattcgga gcttccttca ccacgtttct ggcattttct gaaagtcctc ttgtcagaag
     7021 tagtgttttc acaccacttt taattttgcc tgtcaaatgt gtttgaaggt ggtcatcatt
     7081 aaaaccatag ccgatacata aaattgaaga agcagaatca atagcttcat ttgctttttc
     7141 tcgatgagca tcaaatggtt gtccatagcc ttttttatac ttcccaacac caggcgtaat
     7201 aattaaaggc tcttccactt tacctataca acttgtcatt ggaacgtctc cagccataaa
     7261 ccaatctaaa ctaccatgcg gtttaaagat tttgatatga tctcgataaa ccaattttgg
     7321 acatttacct tttactctaa tgtctcttat ctgcaatttc ctactcaaat caggattatg
     7381 ttttccccaa taacgaccga ccatcattga atctattccc caattttcat actctgccgc
     7441 aaactcaatc aatctatcat aattagtagt aattacacgg gcaattttag gatttgaagc
     7501 tgaaatatgg ggaagcaaat aagaaaattt gagcttctga ttctcagcaa tacatttgtt
     7561 tatcgctttt tgctcttcgg agagtacata ctcggctgtt attttaataa tagcggcttc
     7621 tatcgtctca tttgcttgat gcttcaacaa tgcaccttcc aagccttcag aatccagaca
     7681 atcagaaatg gtattccatg ttaccttatc tatatcatca agacattcag gcattcgttc
     7741 ttttaatcta tcagctaatg ctcccattcc cggcaaacct tcggcacagg acaaacccga
     7801 accgacaata atgaccaaat tgtcagtaaa aatagtttgc aaccattttt ttaattcatc
     7861 ttcatctaat aatggttctc tcattttacg tttcccttcc aaatatctat tcttacaaac
     7921 tagctgttta ctacgagacg tcataggcta tttggtatat taaagtatat ctatgcaaaa
     7981 ttaaacttat aaagaagtta aaataaacat tcatctataa aaccaatttc tccgactcaa
     8041 gaaaatccac attctcccaa ctcaaccccg ccacttcatc atacaaatcc attccaatta
     8101 agcagtaaaa ctgtttgccg aagttggttt cattaatggt ttcaactctg ccagcgttat
     8161 agagttcggc taatgctttt tccggtactt gcacagtgta aggctggagt tttcttaaaa
     8221 taccgcctac ttgatccgca tagcgtaaac ggtcaataag gttttcggct tcttggtcga
     8281 aagggataat caccggaatt aaatggcttg tgatcattcg gaaatcttca gctatttttt
     8341 ggaatgggaa attaaggctt tgcccagcgt cgtgacacgc ctttaaaata tttggcttat
     8401 ccagctcttt tcctttagcc tcataaaccg cttggaaata atgattgatt gctttcggat
     8461 taagtagatc atctgcgtga acctgaaccg ttgaacgcat acagccagaa agtaagccta
     8521 gctctggtgg tgctttccac tgttcttcag gttggaaaac ccaaacattg ctttctacag
     8581 ataaacgttt cccctctcgg ttgcaacgcc ctgctgcttg agccactgaa tctagtcctg
     8641 cttcagctcg cataaccagt gggaaatcga catcaacacc ggcttcaatt aaagaagtgg
     8701 caatcactcg gcaaggtagg ttttgtttta ggcgaagacg aatttcagtc agcgtttgtg
     8761 agcggtgttt agcacacatt agcgtggtga ggtgataagc tccctctaac tgtttcgctg
     8821 aatcatataa cgctcgggcg tgacggcggt tattcacgat tatcagcatt tgtgggttat
     8881 ctgcaagttt gctttgcaac tccacatctg tttgaatacc gatatgcttt accgtagtac
     8941 gtttcaattt ttcaaaaagt gcggttggat taggggcaat ttctcgcaca ttttcaaatc
     9001 ctttataaaa accgttcgct tgatgaatag cgggttgtgt cgctgtgcaa agcactaccg
     9061 aacattgata attgcgagct aattcatcaa tcgcagccat aattgggcgt aataaattca
     9121 acggtagcat ttgggcttcg tccaaaataa tcacgctgcc ggcgatgttg tgcaatttgc
     9181 ggcaacgtga agaacggtcg gcaaacaaac tttcaaagaa ttgcactgca gttgtaacca
     9241 caacaggagc atcccaattt tccgatgcca atttcagctt atctcggctt gatttatcag
     9301 gtaggctatc gtgatcaaaa gtgctgtgat gctccaatac agcctcttct cccagctcgc
     9361 caaaagcttt acgaaattcc gcagcatttt gttcaataat gctggtaaac ggaataacat
     9421 aaataatgcg gcataaacca tactgtttag catgctcaag ggcaaacgcc attgaagtaa
     9481 aggttttacc accgcccgtt ggcacagtca gagaaaacaa gcctgtttcc tgtttcactt
     9541 gagatacggc ataatccaaa atctcactcc gcaagcggtt tacttccgct ttacgttgtg
     9601 cttcctgcgt attaggcgtt tgaatggtaa ttcgctccct aaattgtgca ataaattgat
     9661 taaattgctg atggagtgcg gttaaattgg gggaatttcc tcgttcaagc ggtctttttt
     9721 cgagattggc ataaaaagct tctgtatcaa gaaagtcggc atcgacaagg caagaataga
     9781 gcattcgaat aaaaaaggca taagaaaaga aaggttcgtt tttagaaggc tttaacggcg
     9841 gtaagggcaa tttttcaggc aaattaagtt cttgctgcca aatagcgtca agtaccggta
     9901 gatctttatg accatagcca aattgctctt gcaaacgttg tgtgagtgta gaacgatttt
     9961 tcccttctcc agaaccattt gctaacccag cgtggtgtcc tgcaatgcaa aacgccatca
    10021 ttttgcccag aggcccccaa cgttccactg caatttttgc accggcagta gagtgattta
    10081 cccgttctcc gccatgtaaa cgcttttggt aacgttcact atatttgccc aaatcgtgca
    10141 atagccccgt gcagcgagca atttcttgtg aagcaaacgg cgcagcaaat tcggcggcaa
    10201 gcaagccaac attattggcg tggctgagta aggtttgcca atctgaatga tctgaaagct
    10261 tccccgaatg ggcatacaaa gactgaatag tatgttcttc cattttatac cttcaatatt
    10321 tccttcctaa cacaagcaat actacctcta accttttact ctgtatatat cctagataat
    10381 attttcttaa tctaaatcaa acacaaattt cacttgcaaa attcaagtaa aatttgaccg
    10441 cctgtaacca ttttttctaa gttgtgaacc ctgttctttc agcagaaatt tcacaaaaaa
    10501 tttacttatc tatggggatt tttgctagaa tgttgccatt tttacgacaa gttaaaggta
    10561 ttatcatgtc agaacaaact tttattaccg gtaaagatgc tgcacttgaa gacagcattg
    10621 cgacctttca gcataaatta aaaac
//
LOCUS       DANKBV010000071        10591 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.071, whole genome shotgun sequence.
ACCESSION   DANKBV010000071 DANKBV010000000
VERSION     DANKBV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.071"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..174)
                     /locus_tag="PWY43_001542"
     CDS             complement(<1..174)
                     /locus_tag="PWY43_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HDL2136411.1"
                     /translation="MTACKNGLGFLVLRLFLAYEFFEAGLSKLNGENWFASIQESFPF
                     PFNVLPADLSWAIA"
     gene            complement(483..1196)
                     /locus_tag="PWY43_001543"
                     /pseudo
     CDS             complement(483..1196)
                     /locus_tag="PWY43_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1215..4295)
                     /locus_tag="PWY43_001544"
     CDS             complement(1215..4295)
                     /locus_tag="PWY43_001544"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015831.1,HMM:NF017476.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL2136412.1"
                     /translation="MPNFSFTHLYKKHTKELPAVTLLSALIGLSQVSYANPLPLGNTT
                     GTNNLAVGEGSFALGTGSQAIGKNAIATSGNISPQEFKAELENFRNLVNQIEELRTQI
                     AEQEKQGSVNQQLQDTLKSQIAQYDTILERVREKKAQQAILANSVIPMRKELVNRRNQ
                     LTELENNFNTSFLPEKNKQEAYQKFSTVIESLDWNKLSATNGVDQLATDLKTKIETDF
                     PNINFSNDQYKDLIKGYRNIQGNLVTAIPKFLNKVNDDFAISNSDRGLYYNYLPYHGI
                     DRWEGSNFNFRTLNGVYYDDNLTSTELLRKFMIQRDYNTLSELSRAHAGNADGLAKNY
                     HLVVAKMSYENLKKELSKNSDSKFYKLKKLYEAYGEFSNLNEYVSSSFQGNPLELLTV
                     PVLKKSLENQELLPNKLYRKFNIDNQAIAQTTSNITKEEIADLEKTVENFNKFKELID
                     YSSNAWIFNKEEYRTYMNDNVIPFMEKVEEFTKTLKTLSREDLSEEERTAKTIDSVKL
                     RIYLNEQARNRKNYINGFVPTDWISEITDQMTKSKNLWLKYEEESKTTLKPYQEETIS
                     SLIKDEINKKMAEIDTTTLEIANKERELEELENQINAFTLTDEEKLAEDVKNDLISKL
                     DEAKAALAKNQQTLKEKHDELLGLNNKLDSSPLVKNGKNALAEGTNAFASGENAIAFG
                     TDSQATGNNAIALGANSKANAESAIAIGKGAQALKEKALALGENAIANGASAIAIGDN
                     IGVSGEKAVGIGSNAIVSGNGAISIGSDNRVSHKNAVAIGSNITQTAENSVNLGNESA
                     TTVELTAETAGTTQYAHSEILGEIYKFAASEPAGVVTVGAKGKERRIQNVAAGLVSET
                     STDAVNGSQLYAVAKAANEGKIGVVRYTNTDGVVRIANHLDGNEVNISNKSGNTRLLT
                     GLSEGLAPTDAVNKAQLDREIGVVNNKISAVEKEVKKVKGSVSNAIAIASLPQVSVPG
                     KRQLSVATGHTLGTTSVAVGLNGLSDNGRISYKLGTSVSQNSNFAVGAGIGFSW"
     gene            complement(4490..5719)
                     /locus_tag="PWY43_001545"
     CDS             complement(4490..5719)
                     /locus_tag="PWY43_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDL2136413.1"
                     /translation="MQFREINPSPRLLMCPGPTDADPRVLRAMSAPILGQFDPEFTSL
                     MNETMEMERRLFQTKNEQTYVVNSTSRGGLETVLTAAICPGDKVLIPAFGRFGYLLNE
                     ILARSGADITIIEREWGTVFEPEEIEAELKKGGYKAVAIIHGETSTSMMQPLEEIGKI
                     CKQYDAMLIVDTVATLGGVDIRVDEWGIDACIGGTQKCISAPSGTALITYNKRIEEII
                     AKRKRVEKGIRAESDIDGTLPPIPSNYLDLAQLQDYWSPRRLNHHTEATSAQYGVHEA
                     LRIILQEGLEARFARHILNDKALCAGLEAMGLKIFGDRKHKAPVVTAFHIPEGMSGPA
                     LRSDILNHFGIELATSFGPLDGKVIRIGNMGFSSQKRNVLLTLGALEAVLLSHKVKVP
                     SGDAVAAALAVYKEADK"
     gene            5992..7785
                     /gene="lepA"
                     /locus_tag="PWY43_001546"
     CDS             5992..7785
                     /gene="lepA"
                     /locus_tag="PWY43_001546"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL2136414.1"
                     /translation="MQNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLNYKAKDGEIYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAIEMDLEVVPILNKIDLPAAEPERVAEEIEDIVGIDAIDAVR
                     CSAKTGLGIEDVLEEIVKKIPAPEGDAEAPLQALIIDSWFDNYLGVVSLVRVKNGVLK
                     KGDKIKVMSTGQSYNVDRLGIFTPKQVDTAELKTGEVGWVVCAIKDILGAPVGDTLTH
                     HHNPATEVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLNDASLFYEPETSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVVQTSGETIYVDSPSKLPA
                     VNNIAEIREPIAECNMLVPQEFLGNVITLCVEKRGVQTNMVYHGNQIALTYEIPMGEV
                     VLDFFDRLKSTSRGYASLDYGFKRFQTADMVRVDIMINGERVDALAIIVHRANAAYRG
                     RELVEKMRELIPRQQFDIAIQAAIGNHVIARSTVKQLRKNVLAKCYGGDVSRKKKLLQ
                     KQKEGKKRMKSLGNVEVPQEAFLAILHVGKD"
     gene            7872..8831
                     /gene="lepB"
                     /locus_tag="PWY43_001547"
     CDS             7872..8831
                     /gene="lepB"
                     /locus_tag="PWY43_001547"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2136415.1"
                     /translation="MAQFMPIIFLGIIYGIWQWLDSMQLPNTISIILVALVIICGGFW
                     AFYKFSADPRRKQAIVLQQKRLGRELTAEEIAEIQPKSAVGEFFASLFGILFFVTVLR
                     SFLFEPFQIPSGSMEPTLRVGDFLVVNKFSYGIKDPIWQNTLIETGKPERGDVIVFKA
                     PAQPHVDYIKRVVAVGGDKIKYDFATQQLTVTKANGETKVFEYSEGKPNPEFFYHGEM
                     QVERTEKGNVTHQILNHPMAFNYAPYFYQQEGQAEGEWVVPQGYYFVMGDNRDNSEDS
                     RFWGFVPERNIVGKATFIWLSLDKKANEFPTGLRFSRMFTSIN"
     gene            8877..9551
                     /gene="rnc"
                     /locus_tag="PWY43_001548"
     CDS             8877..9551
                     /gene="rnc"
                     /locus_tag="PWY43_001548"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL2136416.1"
                     /translation="MQLERLQKKLGYQFHNLDYLIQALTHRSAATKNNERLEFLGDSI
                     LNFTIGKVLYDKFPKSNEGELSRMRATLVREQTLAIIARKFDLGDYLKLGPGELKSGG
                     FRRESILSDCVEAIIAAIYLDQDMAKAMEKVEDWYSDLLKDISPGDSQKDPKTRLQEF
                     LQGRKLPLPEYDVLDIKGEAHNQTFRVTCKVINVEEVFIGIGTSRRKAEQNAAEKALA
                     VVKTKK"
     gene            9654..10571
                     /gene="era"
                     /locus_tag="PWY43_001549"
     CDS             9654..10571
                     /gene="era"
                     /locus_tag="PWY43_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL2136417.1"
                     /translation="MTEQIQTPAKTYCGFIAIVGRPNVGKSTLLNKILEQKISITSRK
                     AQTTRHRIVGIHTEDQYQAIYVDTPGLHIEEKRAINRLMNRAASSAIGDVDLIIFVVE
                     GTKWTEDDEMVLNKLRSAKAPVLLAINKIDNIKEKEELLPHITELSQKFDFAEILPIS
                     AQRGKNVHILQKFVRQSLRQGEHHFPEEYVTDRSQRFMASEIIREKLMRFTGEELPYS
                     VTVEIEQFKMNERGVYEINGLILVERDGQKKMVIGAGGQKIKTIGIEARKDMERLFDN
                     QVHLELWVKVKAGWADDERALRSLGYMDE"
BASE COUNT         3177 a         1936 c         2083 g         3395 t
ORIGIN      
        1 agcaatagcc cagctaagat cggctggtag tacgttaaag gggaatggga agctctcttg
       61 aattgaggca aaccaatttt caccgtttag ttttgataac cctgcttcaa aaaattcata
      121 agcaagaaat agacgtaata caagaaagcc caagccgttt ttacaagctg tcattgagca
      181 agctgactga gttgaaaatg atgacataaa aatgctccta actaaaaatg tggttattag
      241 acagaataag tatacgttta ttacaaaaaa tgtgtgaaaa attataacct gtttttatag
      301 ataaataaaa ataggttaaa ttcagtgcaa aatttaacct atttgttttt ttgcaagggt
      361 atttaattag ctttagctag tttatattgc tgtttatcag gagctgtaat taaaatttct
      421 ttcggcggta ctttctgacg atgtaaaatt ccaactacgt tacctacgat agcgatatcc
      481 tgaaattgac cttcaacttt atatttactc atatcaatgc catctttaaa aattaagaag
      541 gtgctgattt tagtactacg gatattttct aaccctaatg cgtgttggtt aaccacatta
      601 taggtaggca cttttcgttt agcatattta ctaattaatt tcgtatcttg actcaatgct
      661 aaaatatcac cgagcttttt ttcttcagca ctgctaacct cgctatatga tcctttattt
      721 gattgaggag gtacagagat aatattacgc tctttctctt cttgaactag agctttatta
      781 tcatagacca catccccaat aaattgagtt ggagctaaac tgacattgtt gccaactaaa
      841 gccaacacta ctttatcatc tagcatatca aagcgtaatt gttggttact attttgctgt
      901 ttaagttgct ggtttatttg atcgagcaaa tgttgcaagg tatcgttatc ggtctgtttc
      961 aactttactt gaatagatga attatgctgt atggcataat aaccaattcc taacttctta
     1021 gcgagttgtt gagagagatc accaacgtta cctttataag ttaccgcaat acgtgcttgc
     1081 tggtattttg ttaagctcgc ctcatcagca taagagaaac ctaatggagc taaaagtaac
     1141 atagcagcaa gtattttgtt tgcctttaca aaatgagaat tcattttttt agtcatacaa
     1201 tacattatgt ttccttacca tgagaaacca ataccagcac caaccgcaaa attactgttt
     1261 tgcgatacag atgtgcctag cttgtaagat attctaccgt tatcactaag gccatttaaa
     1321 cctactgcaa ctgaagttgt accaagggta tggcctgtag cgacagagag ttgacgtttt
     1381 cctggtacag atacttgagg taagcttgct attgcgatag catttgatac cgaacctttc
     1441 acttttttta cctctttttc tacagcactg attttgttat ttactacacc tatttcacga
     1501 tcaagctgtg ctttatttac agcatctgtt ggtgcaagac cttcagaaag tccagttaag
     1561 aggcgagtat tgccggactt attgctgata ttgacttcat taccatctaa atggttagca
     1621 atccttacta caccatctgt atttgtatat ctcacaacac cgattttacc ttcattggct
     1681 gctttggcta cggcatataa ttggctacca tttactgcat cagtactggt ttcagaaact
     1741 aaacctgcag caacattttg aatacgacgc tctttacctt ttgccccaac agtcacgaca
     1801 ccagcaggtt ctgaggcggc aaatttatag atctcaccta aaatttcgga atgtgcgtat
     1861 tgcgttgtgc cagctgtttc agcggtaagt tctacggttg ttgctgactc attgcctaaa
     1921 tttactgagt tttcagccgt ttgggtaata tttgaaccaa tagctactgc atttttatgc
     1981 gaaacgcgat tatcagaacc aatacttatt gccccattgc cagatacaat agcatttgaa
     2041 ccaataccta cggctttttc accagaaaca cctatattat caccaatcgc aatagcagat
     2101 gccccgttgg caatggcatt ttcgcctaaa gcaagggctt tttcttttaa tgcttgtgca
     2161 cctttaccaa ttgcaatcgc actttctgcg tttgcttttg aatttgcacc tagtgcgata
     2221 gcgttattac ccgtagcttg tgaatccgtg ccgaatgcga tggcattttc accagaagca
     2281 aaggcattcg tgccttctgc aagtgcattt ttaccgtttt taacaagtgg tgagctatct
     2341 aatttgttat ttagccctag taattcatca tgcttttctt tgagcgtttg ctggtttttt
     2401 gctaaggctg ctttagcctc atcaagcttt gagattaaat catttttcac atcttccgca
     2461 agtttttctt catcagttaa agtgaaagca ttaatttggt tttcaagctc ctcgagctcc
     2521 ctttctttat tagcaatctc tagtgttgta gtatctattt cagccatttt tttattaatt
     2581 tcatctttta taagactaga tattgtttct tcttggtatg gttttaatgt tgttttggat
     2641 tcctcttcat attttaacca tagatttttg gattttgtca tttgatctgt aatttcagaa
     2701 atccaatcgg taggtacaaa tccattaata taatttttac gatttcgtgc ttgttcattg
     2761 agatatattc ttaatttaac ggagtcaatt gttttggctg tacgttcctc ttcacttaag
     2821 tcttctctag ataaagtttt taatgtttta gtaaattcct ctaccttctc cataaaagga
     2881 attacattat cattcatata agtgcgatat tcttctttat taaagatcca cgcattgcta
     2941 ctataatcaa ttaactcttt gaacttattg aaattttcta cagttttttc taaatcagca
     3001 atttcttctt tagtaatgtt tgatgtggtc tgtgcaatag cctggttgtc aatattaaat
     3061 ttcctataca gcttgttagg gagtaactct tggttttcta aacttttttt aagcacaggt
     3121 actgttaata attcaagtgg atttccttgg aaactgcttg aaacatattc atttaaattt
     3181 gagaattcac cataggcttc atatagcttt ttcagtttat aaaatttaga gtcggagttc
     3241 ttggataatt cttttttcag gttttcatag gacattttcg caactacaag atggtaattt
     3301 ttagccagac catcagcatt tcctgcgtga gcacgactta gttcagaaag agtgttataa
     3361 tctctttgaa tcataaattt tcgcaggagt tctgttgaag ttagattatc atcataataa
     3421 actccattta gtgttcgaaa gttaaaatta gaaccctccc aacgatcaat accatgataa
     3481 ggaaggtagt tataatataa gcctctatca ctattactta tagcaaaatc atcattaact
     3541 ttattcagga atttaggaat ggctgttact aagttacctt gaatattgcg ataaccttta
     3601 atgagatctt tatattggtc atttgaaaaa ttaatattag ggaaatcagt ttcaatttta
     3661 gtttttaagt ctgttgctaa ttgatctacc ccattagttg ctgataattt attccagtct
     3721 aatgattcta ttactgtaga aaatttttga tatgcttctt gtttattttt ttctggtaaa
     3781 aaactagtat taaaattatt ttccaattct gttaactgat ttcttctatt aactaattct
     3841 ttccgcattg gtataacgct attggctaat attgcttgtt gtgctttttt ttcacgtaca
     3901 cgctctaaaa tagtgtcata ttgagcaatc tgactcttta aagtatcttg taattgttga
     3961 ttaacagagc cttgtttttc ttgttcagca atttgagtac gtaactcttc aatttgatta
     4021 actaaattac ggaagttttc taactccgcc ttgaattcct ggggggagat attaccactg
     4081 gttgcaatgg cattcttgcc gattgcttga gaacctgtgc ctaacgcaaa actaccttca
     4141 cctacagcaa ggttattggt gccagtggtg ttgcctaaag gaagtggatt ggcataagag
     4201 acttggctaa gccctatcag agcgctaagc aaggtaacag cagggagctc ttttgtgtgt
     4261 tttttataca gatgggtgaa actaaagttt ggcatatcaa aatttcctca ttaaaacttt
     4321 tacttttcat tcttaaaaaa aatggcccgt actataatat ttaatttgat taaatgaaat
     4381 aggaaaagat taaatttgtg aaaatatttt gagtttctgt ggttttactg tggataaata
     4441 tccacagtat taattattag tgagttgtat tgtaaattta ggaaggtggt tatttatcag
     4501 cctctttata gactgccaag gcagctgcaa ccgcatcgcc tgaaggcact tttactttat
     4561 ggcttaaaag tacggcttct aacgcaccta aggttaaaag tacgttacgt ttttggcttg
     4621 agaaacccat attaccgata cggattactt ttccatctaa ggggccgaat gaggtggcta
     4681 attcaatacc gaagtggttt aaaatatcac tacgcaatgc tgggccactc ataccttctg
     4741 gaatgtggaa tgccgttaca acgggggctt tatgtttgcg atcgccaaaa attttcaaac
     4801 ccattgcttc taaacctgca cataatgctt tgtcgtttaa aatatggcga gcaaaacgag
     4861 cttctaagcc ttcttgtaaa ataatgcgta acgcttcgtg tacaccatat tgtgcagaag
     4921 tggcttctgt gtggtgattt aaacggcgtg ggctccagta atcttgtagc tgtgccaagt
     4981 ctaagtagtt acttggaatt ggcggtaatg tgccatctat atcagactct gcacgaatgc
     5041 ctttttccac acgtttacgt tttgcaataa tctcttcaat ccgtttgtta taagtgatta
     5101 aagctgtgcc tgatggggcg gaaatacatt tttgtgtacc gccgatacaa gcatcaatgc
     5161 cccattcatc cacacgaata tctacaccgc ctaaggttgc aacggtatct acaattaaca
     5221 ttgcatcata ttgtttacag attttgccga tctcttctaa cggttgcatc atagaggttg
     5281 aggtttcgcc gtgaataata gcaactgctt tatagccgcc ttttttcaac tctgcttcaa
     5341 tttcttctgg ttcaaatacg gttccccact ctctttcgat aatggtaata tctgcaccgc
     5401 tacgagctaa aatttcattt aataaataac caaaacgacc aaatgcagga attaacactt
     5461 tatcgcctgg gcaaatcgct gcggttagaa cagtttctaa gccaccacga gaggtggagt
     5521 tgactacata agtttgctca tttttggttt ggaataaacg gcgttccatt tccattgttt
     5581 cattcattaa tgaggtaaac tcagggtcaa attgccctaa aatgggtgca gacattgcac
     5641 gcaaaacacg tgggtcagca tctgttgggc cagggcacat taataaacga ggggagggat
     5701 taatttctct gaattgcata attatttcct ataatcgaat gaattaaaaa aacttatgga
     5761 ttctacaata aaaatttgtg attgctcaat gttttttata ttttagagaa attatttgca
     5821 attagggtga gtgcaagcgg tcatttttct caagaaattt gcacattttt aggaaaaaat
     5881 aaccgcttat tgctatttgt tttctttgca aaaccttgta tttcaagtac aattccccga
     5941 ttaatttttg ataaacaaat aacaaaacag gacattaact gcccgatgaa aatgcaaaat
     6001 attcgtaact tttcgattat cgctcatatc gatcacggca aatcgacttt atcagaccgc
     6061 ttaattcaaa cttgtggtgg cttatcagat cgtgaaatgg aggctcaagt attagattca
     6121 atggatcttg agcgtgagcg tggaattact attaaagcac aaagtgtaac cttaaactat
     6181 aaagcgaaag atggcgaaat ttatcagctc aactttattg atacgccagg gcacgtggat
     6241 ttctcatatg aagtttctcg ctcgcttgct gcttgtgaag gtgctttatt agtggttgat
     6301 gcagggcagg gcgtggaagc acagacgttg gcgaattgtt ataccgcaat tgagatggat
     6361 ttagaggttg tgccaatttt aaataaaatc gatttaccag cagccgagcc tgagcgtgtg
     6421 gcagaagaga ttgaagatat tgtcggcatt gatgccattg atgcagtacg ctgttcagct
     6481 aaaacaggtt taggcattga agatgtttta gaagaaatcg tgaagaaaat tcctgcacca
     6541 gaaggcgatg cggaagcacc attacaagcg ttaattatcg actcttggtt cgataactat
     6601 ttaggcgttg tgtctttagt gcgtgtgaaa aatggcgtgc tgaaaaaagg cgataaaatt
     6661 aaagtgatga gtacagggca gtcttacaat gtagatcgcc ttggtatttt cacgccaaaa
     6721 caagtggata ccgcagaact taaaactggg gaagtaggtt gggtagtttg tgcgattaaa
     6781 gatattttag gggcaccagt gggcgataca ttaacccacc accacaaccc agcaactgaa
     6841 gtattaccgg gctttaagaa agtaaaacca caggtttatg cagggttatt tccgattagt
     6901 tcagacgact acgaagcctt ccgtgatgct ttaggcaaat taagcctaaa tgatgcgtca
     6961 ttgttctatg agccagaaac ttcaagtgcg ttaggctttg gtttccgctg cggcttctta
     7021 ggcttattac atatggagat cattcaagag cgtttagagc gtgaatacga tcttgatttg
     7081 attactaccg ctccgaccgt agtttacgaa gtggtgcaaa ccagtggcga aacgatctat
     7141 gtggatagcc catcgaaact cccagcagtc aacaatattg cagaaattcg tgagccgatt
     7201 gcagaatgta atatgcttgt gccacaagaa ttcctaggca acgtgattac cctttgtgtg
     7261 gaaaaacgtg gcgtacagac caatatggtc tatcacggta accaaatcgc cttaacttac
     7321 gaaatcccaa tgggcgaagt agtgttagac ttcttcgacc gcttaaaatc aacctctcgt
     7381 ggttatgctt cactcgatta cggttttaaa cgtttccaaa cggctgatat ggtgcgtgtg
     7441 gatattatga ttaacggcga gcgtgttgat gcgttggcga ttatcgttca ccgtgcaaat
     7501 gcggcgtatc gtggacgtga attagtggaa aaaatgcgtg agctgattcc tcgccaacaa
     7561 tttgacatcg caattcaagc ggcaattggt aaccacgtta ttgctcgctc aacggtaaaa
     7621 cagttgcgta aaaacgtatt agcaaaatgt tacggtggtg atgtaagtcg taagaagaaa
     7681 ctgctacaaa aacaaaaaga gggtaaaaaa cgaatgaaat ctctcggtaa cgtagaagtg
     7741 ccacaagaag catttttggc aattcttcat gtgggtaagg attaattgtc aatattcgtg
     7801 taaaatacga ccgcttgcaa gcggtcatat tttgagaaaa ctttgcaatt ttgaataaaa
     7861 aggagcattc agtggcacaa tttatgccaa ttatttttct aggtattatt tatggtattt
     7921 ggcaatggct tgattcaatg caactgccaa atactatttc cattatttta gttgccttgg
     7981 ttattatttg tggtggattt tgggcgtttt acaaattcag tgctgaccca agacgtaaac
     8041 aggcgattgt tctacaacaa aaacgtttag gcagagaatt aaccgctgaa gaaatagcgg
     8101 aaattcaacc taaatccgca gtaggcgaat tttttgcctc acttttcggc attttattct
     8161 ttgttacagt acttcgctcg ttcttatttg aaccatttca aattccaagt ggttcaatgg
     8221 agccaacctt gcgtgtaggc gatttcttag tcgtaaataa attcagctac ggcattaaag
     8281 atcctatctg gcaaaacacc ttgattgaaa cgggcaaacc agaacgaggc gatgtgattg
     8341 tattcaaagc accagcacaa ccacacgttg attatattaa gcgtgtggtg gcagtaggtg
     8401 gcgataaaat taaatatgat tttgcaactc agcaattaac ggttactaaa gccaatggcg
     8461 aaacaaaggt atttgaatac agcgaaggca agccaaatcc tgagtttttc tatcacggtg
     8521 aaatgcaggt agaacgcact gaaaaaggta atgtaactca tcaaatttta aatcacccaa
     8581 tggcatttaa ctatgcacct tatttctacc aacaagaagg gcaagcagaa ggtgaatggg
     8641 ttgtgccaca aggatactat tttgtgatgg gtgataaccg tgataacagt gaagacagtc
     8701 gtttctgggg ctttgtgcct gagagaaaca tcgttggtaa agccactttt atttggttaa
     8761 gtttagataa aaaagcaaat gaattcccaa cggggcttcg tttttctaga atgtttacct
     8821 caatcaatta atcttaacag gggcgtatga gatacgcccc tttattcaca taaactatgc
     8881 aactagaacg attacagaaa aaattaggtt atcaatttca taaccttgat tacctcattc
     8941 aggccttaac acaccgcagt gcggcaacca aaaacaatga gcgtcttgaa tttctaggcg
     9001 attcaatttt aaattttact attggtaaag tattgtacga caaatttcca aaatcgaatg
     9061 aaggtgagct tagccgtatg agagcaactc ttgtgcgtga gcaaactcta gcgattattg
     9121 ctcgcaaatt tgatttaggc gactacctaa aattaggtcc gggagagcta aaaagtggtg
     9181 gttttcgccg agaatcgatt ttatcggact gtgttgaagc gattattgcg gccatttatc
     9241 tcgatcaaga tatggcaaaa gcaatggaaa aggtggaaga ttggtattca gatttgctta
     9301 aagacatcag tccaggcgat tcacaaaaag atccaaaaac ccgcctgcaa gagtttttac
     9361 aaggtagaaa attgccattg cctgaatatg atgtattaga tattaaaggc gaagctcata
     9421 accaaacttt ccgtgtaacc tgcaaagtga ttaacgtcga ggaagtgttt atcggcattg
     9481 gcaccagccg ccgaaaagcc gaacaaaatg ccgcagaaaa agcgttagcg gtggttaaaa
     9541 cgaaaaagta agatagcgca agcgtcctcg cttgtgctaa agatttaatt aaatggtaca
     9601 agcgtggacg cttgcaccat caaattcatt ccacagaatg aaaggaaaac aaaatgacag
     9661 aacaaattca aactcctgca aaaacctatt gtggttttat tgcgattgtt ggtcgcccaa
     9721 atgtagggaa atcaacatta ttaaataaga ttttagaaca aaaaatctcg attacttcac
     9781 gcaaagcaca aacaacccgt caccgtattg tggggattca caccgaagat caataccaag
     9841 cgatttatgt agatacccca ggcttacaca tagaagaaaa acgtgcgatt aatcgtttaa
     9901 tgaaccgtgc agcaagttct gctattggcg atgtagatct catcatcttt gttgtggaag
     9961 gcacaaaatg gacagaagat gatgaaatgg tgttaaacaa attgcgttcg gcaaaagccc
    10021 ctgttttatt ggcaatcaat aaaattgata acattaagga aaaagaagaa cttctgccgc
    10081 atattaccga gctttcacaa aaatttgatt ttgctgaaat tctgccaatt tcggcacaac
    10141 ggggtaaaaa tgtgcatatt ttacagaaat ttgtgcgtca atcactgcgt caaggtgagc
    10201 accactttcc tgaagagtat gtgacagacc gctctcaacg ctttatggca tcagaaatta
    10261 ttcgtgaaaa actaatgcgt tttacaggtg aagagttacc ttattcggta acggtagaaa
    10321 tcgaacaatt taaaatgaat gagcgtgggg tgtatgaaat caatggcttg attttagtag
    10381 aacgagatgg tcagaaaaaa atggttattg gtgcgggggg gcaaaaaatc aaaaccatcg
    10441 gcattgaagc tcgaaaagat atggaacgct tatttgacaa ccaagtgcac cttgagcttt
    10501 gggtgaaagt aaaagctggt tgggcggatg atgaaagagc attgagaagt ttagggtata
    10561 tggatgagta gtttttttgt cacttttctt t
//
LOCUS       DANKBV010000072        10471 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.072, whole genome shotgun sequence.
ACCESSION   DANKBV010000072 DANKBV010000000
VERSION     DANKBV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.072"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(14..634)
                     /locus_tag="PWY43_001550"
     CDS             complement(14..634)
                     /locus_tag="PWY43_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDL2136418.1"
                     /translation="MKLWYSTTSPFARKALAVIKHHQLDDKVEMLRVTKAMDPNSPHN
                     QDNPLGRIPALQRNCGRWLFGSLLISEYLDQKGNNTPLLPKEGKPRWAVLALHNLADG
                     IMENTMPTLLQERMARPENEWWVSRHEQLMERNIRSFRQLEEALQEFGTELNLGTLTA
                     VALIDWWAFRLDKIGYDLAKNYPNLTAWAEEMNNKYAILAETKPSM"
     gene            complement(636..1289)
                     /locus_tag="PWY43_001551"
     CDS             complement(636..1289)
                     /locus_tag="PWY43_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HDL2136419.1"
                     /translation="MTPTLPICYQHRDFIIINKPEGLSVHKDEAEIGLTELVARQLNV
                     KQVWLVHRLDKITSGLLILALNKEAAARFYHLFEQHKIYKTYWALSDKKPKKKQGRIV
                     GDMQKSRNGAWKLCHSKENPAVTQFISHSVEDSLRHFILQPKTGKTHQLRVAMKSLGS
                     PILGDKLYSGSQADRVYLHAYQLDFEYENEKISVQALPTSGHFWQKICKSNHIKHGE"
     gene            1338..1997
                     /locus_tag="PWY43_001552"
     CDS             1338..1997
                     /locus_tag="PWY43_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2136420.1"
                     /translation="MAVTIYLVRHGRTVWNLEGRLQGSGDSALVEEGIIGAKKTGIAL
                     KHIPFTAAYSSMQKRAQDTANYILAENERSNIPHFHHKGLNEFDFGSWEGMKSVDLQE
                     NDEYWVMKRTPAEYLAKANGGERFEQLYQRVTQAFNQIAELHQNDGKVLIVSHGMTLT
                     LLTAVLKGIAWQDFRNEEKHSFVFNTAITKVEVENGKVTVLEFNNTDHLDNVPTSQYK
                     R"
     gene            complement(2034..3080)
                     /locus_tag="PWY43_001553"
     CDS             complement(2034..3080)
                     /locus_tag="PWY43_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDL2136421.1"
                     /translation="MSKQIFNEEELKTTEHFEPKQEFDLENAIIEEEEKAVEAELIIE
                     ESLKPSRFWIRVLLATLALFGVAVIAQSIQWLIDTFQQREWIYFAFSIVFFIVSLLGV
                     GAIISEWRKLVYLRNHQKNQQMSNQLLLEQLPSNDGEQAVKFCENIANQLNHLPSIAQ
                     AEQLWKSELNEAYNAKEVLYLFSENVLKPIDKQVKQMISRNAAENAVIVAVSPLAIVD
                     VLLMAARNIALVNKITKAYGMELGYISRLKLFKMVLKNMVFAGATEIATDVGMDFFSQ
                     NLTAKLSLRAAQGIGVGLLTARLGIKAMEFCRPVAFQANEKPRISAIRQELLTSVKNT
                     VFAKTETKEKVFSD"
     gene            complement(3080..4033)
                     /gene="dusC"
                     /locus_tag="PWY43_001554"
     CDS             complement(3080..4033)
                     /gene="dusC"
                     /locus_tag="PWY43_001554"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDL2136422.1"
                     /translation="MNPIPNVILAPMQGVLDPFVRNLLTSVNDYDLCISEFVRVVDQR
                     LPKKTFYRLAPELYQGGLTDSGTPVRVQLLGQHPQWLAENAALAIELGSHGVDLNCGC
                     PSKTVNGSNGGASLLKDPELIYRATKAMREAVPSDKIVSVKVRLGWDSVSQCFEIADA
                     VEQGGANEITVHGRTKVDGYRAERINWQAIGEIQQRLSIPVIANGEIWDFESAKNCQK
                     ITACKALMMGRGALNTPNLSKVVKYNVPKMAWNEVLQLLFKYVQMENQFDTGFYHVAR
                     IKQWLRYLDKEYPQAVELFDILKTEHGYDGLKAHIEKAVEE"
     gene            complement(4111..4737)
                     /locus_tag="PWY43_001555"
     CDS             complement(4111..4737)
                     /locus_tag="PWY43_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDL2136423.1"
                     /translation="MDSRSHIIPLFLAIVITVIAVWSGLNPTDRAVWYAEVIPIFIVF
                     ALLIITYPKFQFSGLAYFLMSLWLIMHLIGAKYTFANVPFEWANQFLTPILGEDRNHF
                     DRVAHYIIGFYSFPMAEWLLRKRKCGMGVAFFFSLFFIMSVAAAYEIIEWQYAVIEGG
                     NAGVEFLGSQGDIWDAQKDMLADTLGALTALMIYLIARPDLRIHDRHF"
     gene            complement(4737..5594)
                     /gene="djlA"
                     /locus_tag="PWY43_001556"
     CDS             complement(4737..5594)
                     /gene="djlA"
                     /locus_tag="PWY43_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDL2136424.1"
                     /translation="MQFIGKIVGFFLGYQLFHGLFGGILGAFIGHLADKKLYELGSVR
                     SSLFGKNLTRQSLFTQTTFAVLGHIAKAKGRVTEADIELARALMARLKLDSAAQQLAQ
                     NAFTLGKEPDFPLRQVIQEFREACGARTDLLRFFVEVQMQAALQDGQLDVNEQQILFT
                     IAETMGMSRFQFEQMIAMIMAAQQFRNGGYYQQNGSYHQQSQGGYQGSYQQPAQPSIE
                     AAYTVLGVTAQDDQNTVKRAYRKLMNEHHPDKLAAKGLPDEMMELAKEKAQQIQAAYD
                     LICKVKEWK"
     gene            complement(5642..6352)
                     /locus_tag="PWY43_001557"
     CDS             complement(5642..6352)
                     /locus_tag="PWY43_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL2136425.1"
                     /translation="MWKNRQLFFGYAENRLLLLSRSITGLLGILCGVYIIDHLVLSDV
                     DMIGKLTSFILIILSAIFLKERASLLQWILCLIAFIGALFIIKPAVDIRFMPYLVGVI
                     GSVFAAIAYLCLRLLAKTKKVESPNTIVFFFSAFSTLILLPFVAIDYVVLTNLQTIYL
                     VLAGVTSAVGQFCVTTAYKYAAAKDISIYSYASVLFSAILGLLFFEQYPDMLSWLGYA
                     IIFVSSWLMFVLTKKQSK"
     gene            complement(6367..6519)
                     /locus_tag="PWY43_001558"
     CDS             complement(6367..6519)
                     /locus_tag="PWY43_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136426.1"
                     /translation="MNFWNSLSPLFKAIFCVLLSALSFAIMAAFLRLSGDVPIAQKAI
                     FRNAQH"
     gene            complement(6588..7061)
                     /locus_tag="PWY43_001559"
     CDS             complement(6588..7061)
                     /locus_tag="PWY43_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL2136427.1"
                     /translation="MKKFAKVLMVAVPAFALAACSSSSDDAAANAAANAAANAAGEFG
                     GLTAEQLKAQYNTVYFGFDSYAVEGEYRQLLDAHAQLLAKTQGNVTIAGHADERGTPE
                     YNIALGQRRADAVKGYLAAKGANNTSTVSYGEEKPAVLGHSEADYAKNRRAVLEY"
     gene            complement(7088..8374)
                     /gene="tolB"
                     /locus_tag="PWY43_001560"
     CDS             complement(7088..8374)
                     /gene="tolB"
                     /locus_tag="PWY43_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDL2136428.1"
                     /translation="MKLFSRISGVIGIFSALLTTQVVADSDVRISIDEGVSMAQPIAV
                     VPFKASGVPADVAQIISDDLRNSGKFTPLERSQMPAQPGSAAEVVADQWSAIGIDNIV
                     VGQVSSTGGGYNVAYQLIDTLGTSGTAGGVIAQGSFIVPAAQIRQGAHTVSDEVFEKL
                     TQIRGAFRTKIAYVVQRGVSSYELRVSDYDGFNAFTITTSKEPLMSPEWSPDGSRLAY
                     VTFENKKAQVVVHDIRSGARRVVAALKGHNGAPSFSPDGSRIAFASNQDGELNIYVVG
                     SGGGTPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMSSSGGGASPMGGSGS
                     YNAKVSSDGKNLIMIAGDRVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKG
                     TSKVLQLVSADGRFKANLPGAGGHYKFPAWSPYLTK"
     gene            complement(8390..9613)
                     /gene="tolA"
                     /locus_tag="PWY43_001561"
     CDS             complement(8390..9613)
                     /gene="tolA"
                     /locus_tag="PWY43_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDL2136429.1"
                     /translation="MKSQQDRLELAVIVSILLHILLIGLLLLGSLFTKTELAAAGGSG
                     GDSDSFEAVMVDTGQVAAEYGQLLAEKKGSKASKPKVEPKEEAVEEDVEDKPTPEEIQ
                     KQKQAELAAIQEKQRQQEIERQQKLQEQQKQAQAKQEELKKQQAEEATRKKAAEAAKL
                     KADAEAKRLEAAAKQAEEEKKAKEALEKKLEQQKKAEAEKKLKEQKELKEQQAKEAKE
                     AKEKAEKEAKAKAEKEAKEKAEKEAKAKAEKEAKEKAEKEAKAKAAAEAKAAQAKNNK
                     ALDDFLNSGDIGGGSSKGGNKNSAGSQGSGGTSGLGQGTNVDGNAYGQRIKKLIQSRY
                     RVDPSFAGKQCDVKIFLSRDGTITNYQVISGDKAVCDAAVSAIVATRKVPPAPSDAVY
                     NMFKSPTLDFSLKVK"
     gene            complement(9627..10064)
                     /gene="tolR"
                     /locus_tag="PWY43_001562"
     CDS             complement(9627..10064)
                     /gene="tolR"
                     /locus_tag="PWY43_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDL2136430.1"
                     /translation="MSRRLKRNDIKSEINIVPFLDVLLVLLLIFMATAPIISQSVEVN
                     LPEDTHSQSVSNEDKTPVILEVAGVGEYKLKIDGNYVQSNGQENINESEVVAYAGEQF
                     QKDNNTLFLVAGDKVVPYEEIMKGITLLKDAGVKSVGLMTEGK"
     gene            complement(10206..>10471)
                     /locus_tag="PWY43_001563"
     CDS             complement(10206..>10471)
                     /locus_tag="PWY43_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDL2136431.1"
                     /translation="YIGLFGTVWGIMHSFMGLSAVKQATLQSVAPGIAEALIATAIGL
                     FAAIPAVMAYNRLNLRLGKVEQGYVNFIDEFTTLLHRQAFSKR"
BASE COUNT         2965 a         2402 c         1932 g         3172 t
ORIGIN      
        1 ctatgtcaaa tctctacatg ctcggcttag tctctgccag aatggcgtat ttattattca
       61 tttcctctgc ccaagcggtt aaatttgggt aattttttgc aagatcgtag cctatcttat
      121 ctaagcggaa tgcccaccag tcaattaatg ctactgcggt taatgtgcct aaattcagct
      181 ctgtgccaaa ctcttgcaat gcctcttcta attgtctgaa cgaacggata ttgcgttcca
      241 ttaattgctc gtggcggctg acccaccatt cattctcggg gcgagccata cgttcttgta
      301 ataaagttgg catcgtattt tccataatgc cgtccgccaa attatgtaat gccaacactg
      361 cccagcgagg tttaccctct ttaggtaaca gtggagtgtt attgcctttt tggtcaagat
      421 attcgctaat taacaaacta ccgaaaagcc aacgtccgca gttgcgttgt agggcaggaa
      481 tacgtcctaa cgggttatct tgattatgtg gcgaatttgg atccattgct tttgtcaccc
      541 gcaacatttc gactttatcg tctaattggt ggtgtttaat caccgcaagg gctttgcggg
      601 cgaaagggct ggtggttgag taccaaagtt tcatactatt ctccgtgttt gatgtgattg
      661 gatttgcaaa ttttttgcca aaaatgaccg cttgtcggca aggcttgcac tgagattttc
      721 tcattttcgt attcaaaatc aagctgataa gcgtgcaaat aaacacggtc ggcttggcta
      781 cctgaataga gtttatcacc taaaatcggg ctgcccaaac ttttcattgc cactcttaat
      841 tggtgagttt tgcctgtttt gggttgcaaa ataaaatgcc tgagcgagtc ttcaacagaa
      901 tgagaaataa attgcgttac tgccgggttt tccttactgt ggcataattt ccacgctcca
      961 tttctgcttt tttgcatatc gccaacaatt ctaccctgct ttttcttcgg ctttttatcg
     1021 cttaatgccc aataggtttt gtagatttta tgctgttcaa ataaatggta aaaccttgct
     1081 gccgcctctt tattcaacgc taaaatcagt aaacctgagg tgattttatc cagacggtga
     1141 accagccaaa cctgctttac attcagttgg cgagccacta attccgttaa gccgatttca
     1201 gcctcatctt tatgcacact caagccttcc ggcttattga taataataaa atcacgatgt
     1261 tgataacaaa taggcaatgt tggggtcatt ttgctataat ccttccaatt tttgactatt
     1321 ttagcaagga aattacaatg gctgttacca tttatttagt acgacacggc agaaccgtgt
     1381 ggaatttgga aggcagatta caaggctcgg gcgattccgc tttagtagaa gaagggatta
     1441 tcggagcgaa gaaaaccggc attgcgttaa aacatattcc gtttaccgcc gcctattcca
     1501 gtatgcagaa acgggctcaa gataccgcaa actacatttt agcagaaaat gaacgcagta
     1561 atattccgca ttttcatcat aaaggcttga atgaatttga ttttggcagc tgggaaggaa
     1621 tgaaatctgt cgatttgcaa gaaaatgatg aatattgggt gatgaaacgt acaccggcag
     1681 aatatctggc aaaagcaaac ggtggcgagc gatttgagca actttatcaa cgggtgactc
     1741 aggcgttcaa tcaaatcgcc gagctacacc aaaatgacgg caaagtgctg attgtttcac
     1801 atggtatgac cttaacctta ctcacggctg tgttaaaagg cattgcgtgg caagatttcc
     1861 gcaatgaaga aaaacatagc tttgtgttca atactgccat tacgaaagta gaagtagaga
     1921 atggcaaagt aacggtgctg gaatttaata ataccgacca tttggataat gtgccaacaa
     1981 gccaatacaa acgataatat taagggcgga acattgtccg cccatagcat tttctaatca
     2041 gagaacactt tttctttcgt ttctgtttta gcaaataccg tatttttgac cgaagtaagc
     2101 aactcctgac gaatagctga aattcttggc ttctcattcg cttgaaaagc aacaggacga
     2161 caaaattcca ttgctttaat gccaaggcga gcagtgagta aacccacacc aataccttgt
     2221 gcggcacgga gcgataattt cgccgtaagg ttttgcgaga agaaatccat tccaacatca
     2281 gtagcgattt cggttgcacc ggcgaacacc atatttttca gcaccatttt gaacagtttt
     2341 aagcggctga tataacccaa ttccattcca taggctttgg taattttatt cactaaagcg
     2401 atatttcttg ccgccattaa caacacatca actattgcca gcgggctaac cgccacaatc
     2461 accgcatttt ctgccgcatt gcgggaaatc atctgcttaa cctgtttatc tatcggcttt
     2521 agcacatttt cgctaaacag atatagtacc tctttggcat tataagcctc gttcagttcg
     2581 cttttccaaa gttgctcggc ttgagcaatg ctcggcaagt gattaagctg atttgcaata
     2641 ttttcgcaaa atttgaccgc ttgttcgcca tcattggaag gcagctgttc cagcaataat
     2701 tggttactca tttgttgatt tttctgatga tttcgcaaat acaccaactt tcgccattcg
     2761 gaaataatcg ccccaacgcc caacaagctg acaataaaaa agactatcga gaacgcaaaa
     2821 tagatccact ctctttgctg gaacgtatca atcaaccact gaatactttg agcaattacc
     2881 gccacgccaa acaaggcaag tgtggcaagt agtactcgaa tccaaaagcg ggaaggctta
     2941 aggctttctt caataatcag ctccgcctca actgcttttt cctcttcttc aataatcgca
     3001 ttttcgagat caaactcttg tttaggttca aaatgctcgg ttgtttttag ctcttcttca
     3061 ttaaaaattt gcttactcat tattcctcca ctgccttttc aatatgtgct tttagaccat
     3121 catagccgtg ttcggttttc aagatatcaa ataattctac cgcttgagga tattctttat
     3181 ccaaataacg tagccattgt ttgatgcgag caacgtggta aaaaccggta tcaaactgat
     3241 tttccatctg aacgtactta aacaatagct gtagcacttc attccacgcc attttcggaa
     3301 cattatattt caccactttg cttaaattcg gtgtgtttaa cgccccacgc cccatcatta
     3361 atgccttgca agcggttatt ttttggcaat ttttcgccga ttcaaaatcc caaatttcgc
     3421 cgttggcgat aacaggaata gacagccgtt gttgaatttc gccaatcgcc tgccaattaa
     3481 ttctttctgc tcgataacca tcaactttgg ttcgcccgtg aacggtaatt tcattcgctc
     3541 cgccctgttc taccgcatcg gcaatttcaa aacattggct aacggaatcc caacctaatc
     3601 gcacttttac ggaaacgatt ttatcgcttg gcacagcttc acgcatcgct ttggttgcac
     3661 gataaatcag ttccggatct tttaataacg aagccccacc attgctgcca tttaccgttt
     3721 tagaggggca gccacagttg agatctaccc cgtgtgaacc aagctcaatg gcaagagctg
     3781 cattttctgc cagccactgt gggtgttgcc ctaaaagctg tacccgcact ggtgtgccgg
     3841 aatcggttaa tccgccttga tatagctcag gggctaggcg ataaaaggtt ttcttcggta
     3901 aacgttgatc gaccactcgc acaaattcgg aaatacagag atcgtagtca ttcactgaag
     3961 ttaaaaggtt gcggacaaaa gggtctaaaa ccccttgcat cggggcaaga ataacgttag
     4021 gaattggatt cattggtaaa ataaaaaata caagcggtta ttttttctca acattttgca
     4081 aaaaattggc aagaaacaac cgcttgaagg ttaaaaatgt ctatcgtgaa tgcgcaaatc
     4141 ggggcgagcg atcaggtaaa tcatcagtgc ggttaatgcc ccaagagtat ccgcaagcat
     4201 atctttttga gcgtcccaaa tatccccttg cgagcctaga aattccacgc cggcattgcc
     4261 accttcaatc acggcatatt gccattcaat aatttcgtaa gctgccgcca ccgacataat
     4321 gaaaaacagc gagaagaaaa atgctacgcc cattccgcac ttgcgtttac gcagtaacca
     4381 ttctgccatc gggaagctgt aaaaaccgat aatgtaatgt gctactcgat caaaatggtt
     4441 acgatcttca cctaaaatcg gggttagaaa ttgatttgcc cactcaaacg gcacattggc
     4501 aaaggtatat tttgccccga tcaagtgcat aatcagccaa agcgacatca aaaaataagc
     4561 taacccacta aactgaaatt tagggtaagt gataattaaa agggcaaaaa cgataaaaat
     4621 cggtatcact tccgcatacc aaactgcgcg gtctgtcgga tttaaccctg accaaaccgc
     4681 aatcacggta ataacaatcg ctaaaaatag agggatgata tgcgagcgtg aatccattat
     4741 ttccactctt tcactttaca aattaaatcg tacgccgctt gaatttgttg agctttctct
     4801 tttgccaact ccatcatctc atcaggcaaa cctttagcgg ctaatttatc cgggtgatgt
     4861 tcgttcatta acttacgata agcccgtttc actgtatttt gatcgtcttg tgcggttacg
     4921 cctaatacgg tataggctgc ctcaatgctt ggttgtgcag gctgctgata actgccctga
     4981 tagccacctt gagattgctg atgataactc ccattttgct gataatagcc accattgcgg
     5041 aattgttgag ctgccataat cattgcgatc atctgctcaa attggaagcg agacattccc
     5101 attgtttcgg caatcgtaaa cagaatttgt tgctcgttca catcaagttg cccatcttgc
     5161 aaggctgcct gcatttgcac ttcgacaaag aaacgtaata agtcagttct tgccccacag
     5221 gcttctctga actcttgaat gacttggcgt aacgggaagt caggctcttt acctaaggta
     5281 aaggcatttt gcgctaattg ctgggctgca ctatctaatt ttaagcgagc cattaacgct
     5341 cgggctaatt caatatccgc ttcagtaaca cgcccttttg ctttcgcaat atgccccaac
     5401 accgcaaaag tggtttgggt gaaaagcgat tggcgagtta aatttttacc gaaaagactt
     5461 gaacgaactg aacctaattc atagagtttt ttatcggcta aatgcccgat aaaagcgcca
     5521 agtatcccac caaataaacc gtgaaaaagc tgatagccca agaaaaaacc gactattttt
     5581 ccgataaatt gcattttttt atccttttat agaaatgaat aaacgttatt ctaacaaaat
     5641 tttactttga ttgcttttta gttaatacaa acataagcca gcttgagaca aaaataattg
     5701 cataacctag ccaactcaac atatccgggt attgttcaaa gaacaacaac cctaaaattg
     5761 cactaaatag cacagaggca taactataaa ttgagatgtc tttcgccgcc gcatatttat
     5821 aggcggtagt tacacaaaac tgcccgaccg ctgaagttac ccctgctaac actaagtaga
     5881 ttgtctgtaa attggttagc actacataat caatggcgac aaaaggcaat aatatcaagg
     5941 tcgaaaaggc agaaaaaaag aaaacaatgg tattaggtga ttctactttt ttggtttttg
     6001 ccaataacct taagcataaa taggcaatgg ctgcaaacac agagccaatc acgccaacca
     6061 gataaggcat aaagcgaata tcaactgccg gtttgataat aaaaagtgcc ccgataaagg
     6121 caataaggca taaaatccat tgcaggagag atgctctctc ttttaaaaaa atcgcagaaa
     6181 gaataattaa aatgaacgac gtgagtttgc caatcatatc gacatcactt agcacaaggt
     6241 gatcgatgat atatacacca cataaaatcc ccaataaccc ggtgatggaa cggcttaata
     6301 ataacaaacg attttctgca taaccaaaga ataattggcg atttttccac acaatataac
     6361 cgcttatcaa tgctgtgcgt tgcgaaaaat cgctttttgt gcaatcggta catcccccga
     6421 aagacgaaga aaagcagcca taatcgcaaa gcttagtgcc gacaaaagca cacaaaaaat
     6481 ggctttaaat aacggcgaga gcgaattcca aaaattcata gcataaaggc ttttaataaa
     6541 aataaagccc tgattattca gggcttcaat ttagaatcaa ctaagaatta gtattctaat
     6601 actgcacgac ggtttttcgc gtagtcagct tctgagtgac ctaatactgc tggtttctct
     6661 tcaccgtaag aaacagttga agtgttgtta gcacctttag ctgctaagta acctttaact
     6721 gcatctgcgc gacgttgacc taatgcgatg ttgtactctg gagtaccacg ctcatcagcg
     6781 tgaccagcaa tagttacgtt accttgagtt tttgctaata attgagcgtg agcgtctaat
     6841 aattgacggt attcaccttc aactgcatag ctgtcaaaac cgaagtatac agtgttgtat
     6901 tgtgctttta attgttcagc tgttaaacca ccgaattcgc ctgctgcatt tgctgctgca
     6961 tttgctgctg cgtttgctgc tgcatcatct gatgagctgc tacatgctgc taatgcgaaa
     7021 gctggtactg ctaccattaa tactttagca aattttttca tttgttgctc ctaatagaga
     7081 aatattatta tttagttaag tatggtgacc atgcagggaa tttataatgt ccacctgcgc
     7141 caggcaaatt agctttaaaa cgcccatctg cagacaccaa ttgtagcact ttactcgtac
     7201 ctttggtaga gctataaata accataatgc cattcggtga aatacttgga ctttcgtcta
     7261 agaaagttga actcagtact tctgtgccac ctgaagcgag atcgcgtttc acaactctat
     7321 caccggcaat catgattaaa ttcttaccat cagatgatac tttcgcatta tagctaccgc
     7381 taccgcccat tgggctagca ccaccacctg atgaactcat tctataaact tgaggagaac
     7441 ctgcacggtc agacgtaaag tagattgtgc taccatctgg agaccagctt ggttcagtat
     7501 tatttccagc attagcggtc aatttacttg gtgtaccacc gccactacct acaacataaa
     7561 tatttaactc accatcttga tttgaggcaa acgcaatgcg agaaccgtct ggagagaatg
     7621 atggcgcacc attatgtcct ttcaatgctg ccactacacg gcgagcacct gaacgaatat
     7681 cgtgaacaac aacctgcgct tttttatttt caaatgtcac ataagctaat ctgctaccat
     7741 ctggtgacca ctctggcgac attaaaggct ctttactggt tgtaatagta aaggcattaa
     7801 aaccatcata atcagatact cgtaattcat acgatgatac gccacgttga accacatacg
     7861 caattttagt tctaaatgca ccgcgaattt gggttaattt ttcaaatacc tcatcactta
     7921 ctgtatgcgc accttgacga atttgggctg ccggaacaat aaatgaacct tgggcaatga
     7981 caccacccgc tgtacccgat gtaccaagag tatcaattaa ttgataagcg acattataac
     8041 cgccacctgt gctactcact tggccaacta caatattatc aataccgata gcactccatt
     8101 gatctgccac cacttccgct gctgatcccg gttgagcagg catttgagag cgttcaagcg
     8161 gtgtaaattt accactattt cgcaaatcat cagaaataat ttgtgcaaca tctgccggca
     8221 caccgctggc tttaaatggc acaaccgcaa tcggctgagc catacttaca ccttcatcaa
     8281 tagaaattcg cacatcagaa tcagcaacca cctgagttgt caataatgct gaaaagatgc
     8341 ctattacacc cgatatacga gagaataatt tcatttgtag tcctctaaat tatttaactt
     8401 ttaaactaaa atcaagtgtt ggtgatttaa acatattata taccgcatct gatggtgcag
     8461 gtggtacttt tctagttgcc actattgcac ttaccgctgc atcacatact gctttatcgc
     8521 cactgataac ttgatagtta gtaattgtgc catctcgact taagaatatc ttaacatcac
     8581 attgttttcc ggcaaaactt ggatcaaccc gatatcgaga ttgaattaat ttcttaattc
     8641 gttgaccata tgcattacca tctacattag taccttgccc taagccactt gtaccaccag
     8701 aaccttgtga tcctgctgag tttttgttac cacctttact tgaaccacca ccgatgtctc
     8761 cactattgag gaaatcatca agtgctttat tgtttttcgc ttgggcggct tttgcctcag
     8821 cagctgcctt cgctttagct tctttctcgg ctttctcttt ggcttctttt tctgcttttg
     8881 ctttggcttc tttctcagcc ttttcttttg cttctttttc tgccttggct ttagcttctt
     8941 tttctgcttt ttccttcgct tctttagctt cttttgcctg ctgttccttc agttcttttt
     9001 gctcttttaa ctttttctca gcttccgctt tcttctgctg ttcaagcttc ttttcaagtg
     9061 cttctttagc ttttttctct tcttcagctt gtttagcagc agcctctaaa cgcttagctt
     9121 ctgcatctgc tttcagtttt gctgcttcag ctgctttttt acgagttgct tcttctgctt
     9181 gctgcttttt aagctcttct tgcttagctt gtgcttgctt ttgctgttct tgtagttttt
     9241 gctgacgctc aatttcttgc tgacgctgtt tctcttgaat cgccgctaac tcagcttgtt
     9301 tctgtttttg gatctcttcc ggagtcggtt tatcttccac atcttcttca acagcttcct
     9361 ctttaggctc tacctttggc ttcgatgctt ttgagccttt tttctctgcc aataactgcc
     9421 catattcagc agccacttgc ccagtatcta ccatcacagc ttcaaagcta tcgctatcgc
     9481 caccactgcc accagccgca gcgagttccg tcttagtgaa aagagacccg agcaataaca
     9541 acccgattaa caatatatgc aataagatcg aaacaataac cgctagctct agtctatctt
     9601 gttgagattt cacacgttat ccttggctat ttaccttctg tcattaaacc taccgatttc
     9661 actccggcgt ctttaagtag tgtaatacct ttcataattt cttcataagg tactacttta
     9721 tcaccggcaa ctaaaaataa ggtattgtta tctttttgaa attgttcccc tgcataggca
     9781 acaacttctg attcattaat attctcttgc ccattacttt gcacatagtt accgtcaatt
     9841 ttcaatttat attcaccaac acctgccacc tccaaaatca ctggagtttt atcttcatta
     9901 gaaactgatt ggctatgcgt atcttccggt aaattaactt ctacactttg actgataatt
     9961 ggggccgttg ccataaaaat cagcaataat accaacaata catcaagaaa tggaacaatg
    10021 ttgatttctg atttaatgtc attacgtttt aaacgacgag acatatattc ctctttacag
    10081 gtgcttagca ccttaattga tctgaaataa ccggcacagt gtcccggtct cgaataatgc
    10141 ggttacaagc ggtctttttt acgaaaaact ttacaaattt tcattaaaaa tcaaccgctt
    10201 gtagattatc tttttgaaaa agcctgacgg tgtaataagg tcgtgaactc atcaatgaaa
    10261 ttcacatacc cttgctcaac tttacctaaa cgtaaattca aacggttata tgccattact
    10321 gcaggaatcg ccgcaaataa accgatagcc gttgcaatta atgcttctgc aatacccggt
    10381 gcgactgatt gtaaagttgc ttgcttaact gcacttaatc ccataaagga gtgcataatt
    10441 ccccaaaccg ttccgaataa accaatatat g
//
LOCUS       DANKBV010000073        10420 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.073, whole genome shotgun sequence.
ACCESSION   DANKBV010000073 DANKBV010000000
VERSION     DANKBV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.073"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..931
                     /locus_tag="PWY43_001564"
     CDS             <1..931
                     /locus_tag="PWY43_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDL2136432.1"
                     /translation="LVQYGFRLPSALDNRPLRFEEFERLSPQTIYVSATPGAYELEKN
                     PDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIHKRVAVDERVLVTTLTKKMAEDLT
                     DYLDEHGVRVRYLHSDIDTVERVEIIHDLRMGMFDVLVGINLLREGLDMPEVSLVAIL
                     DADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITNSMQKAITETERRREKQMKYN
                     EEHGITPQALNKKVGELLDIGQSVKPKRGKQAVKKAENSATDYKPKSRKELEKELKTL
                     EQQMRDFAKDLEFEKAAAVRDKLQQLKAYLLEV"
     gene            934..1344
                     /locus_tag="PWY43_001565"
     CDS             934..1344
                     /locus_tag="PWY43_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136433.1"
                     /translation="MISRSLFSKLILFAKIVIFSQLVFMVVIWIAHHIMREYSNPFER
                     FGTLFNNVFLTFIIPMMVSSAVIVYKGYTQKNEDKVFWIYMGFFLIWILGFIYFEPAV
                     YDKSLKISGAGFGAMLSIIICSKLLPKETNKEQL"
     gene            complement(1565..2653)
                     /gene="purK"
                     /locus_tag="PWY43_001566"
     CDS             complement(1565..2653)
                     /gene="purK"
                     /locus_tag="PWY43_001566"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL2136434.1"
                     /translation="MQKSAIYPPVYVLGNGQLGRMLRYAGAPLDIEVKPLAFDAPVFD
                     IEPNSIITAEIERWADTPLTQLLGNHPNFVNLNVFGKLADRFTQKSLLDELHLATSKW
                     QLLENAEHWQDIFQHIGEKVVVKRRTGGYDGRGQWIVTQDSIHQITPELFGEVIAEKF
                     IPFEGEISIVGARFRDGSTRFYPISHNLQQNGILRYSVSDVNLPNQALYQQQAEQMLA
                     TVMQKLEYVGVMAMECFVVDDKLLINELAPRVHNSGHWTQLGCAISQFELHLRALLDL
                     PTPALDQIAPSVMVNLIGIEHNKQWLSLPFSQLHWYGKEVRAGRKVGHINLTHADKNG
                     LIELLEQLRPSLTEDFHSGLDWAINKLK"
     gene            complement(2672..3637)
                     /locus_tag="PWY43_001567"
     CDS             complement(2672..3637)
                     /locus_tag="PWY43_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL2136435.1"
                     /translation="MSKKLLILHTGGTISMKEGEDGKVSPSNENPLLAALERLNHPAK
                     LVQESLFNIPSPHINIEHWQQLKMRIEKAVNEENVDGIVITHGTDTLEETSYFLDLAL
                     SVNIPVVITGAMRSSNELGADGLINLQSAILVALNEESRDKGVLVVMNDEIHNAKFVT
                     KTHTTNVATFQTPTFGPCGLVTKESVIFFQKLTTYERYPIQQVTKANVQLLKAYAGMD
                     SFLLEQLAKSKCDGVVIEALGAGNLPPSCLAGIEALVNANIPIVIVSRAFNGITQDVY
                     DYVGGGKQLKQKGIIFTKGLSGQKARIKLMVLLSQTLDKPLSDYF"
     gene            complement(3652..4149)
                     /gene="purE"
                     /locus_tag="PWY43_001568"
     CDS             complement(3652..4149)
                     /gene="purE"
                     /locus_tag="PWY43_001568"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL2136436.1"
                     /translation="MPNKAEIAIVMGSKSDWATMSEATQILDQFGLSYHVEVVSAHRT
                     PDKLFSFAETAETNGYKVIIAGAGGAAHLPGMIAAKTIVPVLGVPVKSSMLSGVDSLY
                     SIVQMPKGIPVGTLAIGPAGAANAGLLAAQILAAFNPDIAEKLRIFRQTQTQMVLDNP
                     DPRVE"
     gene            4323..4967
                     /gene="sspA"
                     /locus_tag="PWY43_001569"
     CDS             4323..4967
                     /gene="sspA"
                     /locus_tag="PWY43_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDL2136437.1"
                     /translation="MTSSINKRSVMTLFSDKNDIYSHQVRIVLAEKGVPYEIENVMLG
                     SISEDLLELNPYGNIPTLVDRELVLFNPRIIMEYLDERFPHPPLMPVYPVSRGQCRLN
                     MYRIEQDWYSQIDIVNKNPESAEGKKALAQLKEDVLALAPIFAAKPYFMSDDFSLVDC
                     YIAPLLWRMQILGVKFSGATAKPINAYMTRVFQRDSFIQSVGGEAPKHLMDDKD"
     gene            4967..5374
                     /locus_tag="PWY43_001570"
     CDS             4967..5374
                     /locus_tag="PWY43_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDL2136438.1"
                     /translation="MKPLRPYLYNAYYNWIIDSDNTPYLLVNAEFPDVDVPVEFVKEG
                     KIILNISPRSIGQYIVADEYISFSARFQGMLRDIYIPFGAMVAIYAQETGDGIMFQEE
                     AYYTEEAYQARVRAVEQPKKIKAKKTSTLKLVK"
     gene            5571..6530
                     /locus_tag="PWY43_001571"
     CDS             5571..6530
                     /locus_tag="PWY43_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2136439.1"
                     /translation="MKFHKTLLVTLLSSLSVAALAQDKIQNVAITAIVEHPALDSIRK
                     GVIEELAREGFVDGKNIKIDYQSAQGSTATAAQIARKFVGDKADIIIPITTPSAQPVV
                     AATRSIPIVFSGVTDPVAAKLVKSWEPSGTNVTGISDHKAMAPQVKLIQTLVPDLKAV
                     GYVYSAGEVNSAIVLEELKQEAQKQGFTIVPVAVQRSADIGTAARSLNGKVQAIYISE
                     DNAVVSAYEALHKAALEAKIPVIAADRDTVQRGAVAAYAVNQYDIGVATGKVAARVLK
                     GEKAGTIPIQEVSKLELSINTKTAKELGITIPEVLIKEAKQTF"
     gene            6610..6870
                     /locus_tag="PWY43_001572"
     CDS             6610..6870
                     /locus_tag="PWY43_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2136440.1"
                     /translation="MGWIAAIIVGAIIGWIASAVMKSEGGLCKNIIIGIVGSSLGRWV
                     FGDVLGIGSAYSAGDFNIVGIFFGVLGAALLIFILRKLSIIN"
     gene            complement(6920..7810)
                     /locus_tag="PWY43_001573"
     CDS             complement(6920..7810)
                     /locus_tag="PWY43_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2136441.1"
                     /translation="MDKLNAISLFCKVIETQSFTQAAQQAQISLPMASKLVAQLEEHL
                     NVRLLQRTTRKITPTEAGLLYYQQCLPILNELKEADASVSNINSTLQGKITMSVPMDF
                     GSRFIAPYLGKFMEDYPNLALHIEFSDRRVDVIAEGFDLVLRIGSLADSSIVAKKIAD
                     SHLVLVASPDYLARFGEPKGLEALVERGCLLYEYHPQWQFSQQGQKMQIKPQGKIYSN
                     NGYALVQMAKAGLGIINIPKFLIKHELKNGELIPIMPFLTQEPLQISLLYPNRRYLSP
                     KVRILIDFFTQLMQEQKEYI"
     gene            7940..>10420
                     /gene="topA"
                     /locus_tag="PWY43_001574"
     CDS             7940..>10420
                     /gene="topA"
                     /locus_tag="PWY43_001574"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL2136442.1"
                     /translation="MGKSLVIVESPAKAKKINQYLGSNYVVKSSVGHIRDLPKSGSDE
                     KTEKAKPISTKGLTAAEKAKIKAEKERVALVKRMGIDPYNGWKANYQIMPDKEKVVSE
                     LKSLAKKADHIYLATDLDREGEAIAWHLREIIGGDDSRFSRVVFNEITKDAIKKAFDD
                     PQPLNIDRVNSQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREI
                     KAFQSEEFWTILANTQINRKKLPLELTTYKGKKFSASNEAEANVAVRALEQSDFIVSN
                     IEAKETSSRAAAPFITSSLQRAASTRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLS
                     REALAMARGYIQDNFGANYLPEKANVYRSKDNAQEAHEAIRPSDVNVKMADLKGMDKD
                     AERLYDLIWRRFLACQMLPARYDSTAVTVSAGDYELKANGRVLRFDGWTKVLPVQGKN
                     PEDQELPAVELHQKLTLDEIVPSQHFTKPPARFNEASLVAELEERKIGRPSTYASIIS
                     TIQERGYVRTENKRFYVEKIGEVVTDRLNESFKDLMNYDFTANMEEVLDQIAEGSKNW
                     KEELNTFFKHFSEELSQAELDELEGGMKPNSLVPTDIDCPTCGRKMAIRTASTGVFLG
                     CTGYALPPKERCKTTMNLIPEAEFFNVLDSDSETKALMARKRCPKCDSAMDSYVIDAE
                     RKLHVCGNNPNCDGHIIEQGSFKIKGYDGPVVECDKCGSDMHLKLGRFGKYMGCTSCD
                     NTRKILKNGEVAPPKEDPIAFPELKCEKADAYFVLRDGASGVFMSAHNFPKVRESRAP
                     KVVELAQYRERLPEKLQYLADAPQQDPEG"
BASE COUNT         3061 a         1943 c         2335 g         3081 t
ORIGIN      
        1 attggtgcaa tacggtttcc gtctgccgtc tgcactggat aaccgcccgt tgagatttga
       61 agagtttgaa cgtctttcac cacagacgat ttacgtttct gccacaccgg gggcgtatga
      121 attggagaaa aatcccgatg tggtggatca agtagttcgt ccaaccggct tgctcgatcc
      181 gattatcgaa gttcgccccg ttgctacgca ggtggacgat ttactgtctg aaattcacaa
      241 acgagtggcg gtggacgaac gggtgctggt gacaacactc accaaaaaaa tggcggaaga
      301 tctcaccgat tatttggacg aacatggcgt acgagttcgt tatctacata gcgacattga
      361 cacggtggaa cgagtcgaaa ttatccacga tttacgaatg ggaatgtttg atgtgctggt
      421 ggggatcaac ttattgcgtg agggcttaga tatgcccgaa gtgtcgctgg ttgccattct
      481 tgatgcggat aaagagggat ttttacgttc cgaacgctcg ttaattcaga ccatcggacg
      541 tgccgcccga aacctcaacg gaaaagcgat tttatacggc gaccgcatta ccaactcaat
      601 gcaaaaagca attactgaga ccgaacgccg ccgtgaaaag cagatgaaat acaacgaaga
      661 acacggcatc acgccacaag cgttgaacaa aaaagtgggt gaattgctcg acatcggaca
      721 atccgtcaag ccaaaacggg gcaaacaagc ggtcaaaaaa gccgaaaatt ctgcaaccga
      781 ctacaagccg aaatcccgta aagaactgga aaaagagctt aaaacgctgg aacaacaaat
      841 gcgtgatttt gcgaaagatc tggaatttga aaaagcggct gcggtaaggg ataagttgca
      901 gcagttaaag gcgtatttgt tggaggttta aatatgattt ctcgttcatt attttcaaaa
      961 ttaatcttat ttgccaaaat tgttattttc tctcaacttg tttttatggt ggttatctgg
     1021 attgcccacc atattatgcg ggagtacagt aatccgtttg aacgatttgg taccctgttc
     1081 aataatgtgt ttttgacttt tattattccg atgatggtaa gtagtgcggt tattgtttat
     1141 aaaggttata cccagaaaaa tgaagataaa gtgttttgga tttatatggg gttcttttta
     1201 atttggattt tgggatttat ttattttgag ccggcagttt atgataaatc actgaagatt
     1261 tcaggtgcag gatttggggc gatgctctct attatcattt gctcgaagtt attgcctaag
     1321 gaaacaaata aagagcagct ctaagattct tgtgaagaaa tctgttttct cggaggaaga
     1381 aaatgctttt tgatagccat atccaccctg aggggttttc agatcaagat gatgaccgga
     1441 agtagccatt atgttgaatt attttgcaaa ttttagcact aagtcgaccg cttgtttggt
     1501 acgagcgagg aagctcgcac catcgggtaa acagatttgc aaaaaagtta caaaatccga
     1561 ccgcttactt gagcttattg attgcccaat ctaatcccga atggaaatct tccgttaggc
     1621 tcggtctgag ttgttccagt agttcaatta agccgttttt atcggcgtga gtgaggttga
     1681 tatgcccaac ttttctacca gctcggactt ctttgccata ccaatgtagt tggctaaatg
     1741 ggaggcttag ccactgcttg ttatgttcaa tgcctattaa attaaccatc acgcttgggg
     1801 caatttggtc gagtgctggc gtaggtagat ctaataatgc ccgtaaatgt aactcaaatt
     1861 ggctgatggc acagccgagt tgtgtccagt gtccgctgtt atgcactcga ggggcaagct
     1921 cgttgatgag tagcttgtcg tccacaacaa agcattccat tgccattacg ccgacatact
     1981 ctaatttttg cattaccgta gcgagcatct gttcggcttg ctgctggtag agggcttggt
     2041 tcggtaaatt cacatcggag acggagtaac gtagaatgcc gttttgttgt aaattgtgag
     2101 aaatagggta gaaacgggtt gagccgtcac ggaatctcgc cccaacaatg gaaatttccc
     2161 cctcaaacgg gataaatttt tcggcaatca cttcgccaaa taattcaggt gtaatttgat
     2221 gaatcgagtc ttgcgttaca atccattgcc cacgtccgtc atatcctccg gttcggcgtt
     2281 taaccactac tttttcgcca atatgctgaa aaatatcttg ccaatgttcg gcattttcta
     2341 gcagttgcca ttttgaagtc gcaagatgga gttcatcaag tagtgatttt tgcgtaaaac
     2401 gatcggctaa cttcccaaac acattcaggt tcacaaaatt cgggtggtta ccgagtaatt
     2461 gcgttaaagg cgtatctgcc caacgttcga tttctgcggt aataatgctg tttggctcaa
     2521 tatcaaacac aggggcatcg aaagccagcg gtttgacttc aatatcaagt ggtgctccgg
     2581 cgtagcgtag cattctcccg agctggccgt tgcctaatac atacacaggt gggtagattg
     2641 cacttttttg catcgtggtg ttctcaatgg gttagaaata gtctgatagt ggtttgtcta
     2701 aggtttggct gagtaagacc ataagtttaa tgcgagcttt ttgcccactt aagcctttgg
     2761 taaaaataat gcctttttgc tttaactgtt ttccaccgcc tacataatca tagacatctt
     2821 gggtaatacc attgaatgcc cgagaaacaa ttacaattgg aatattggca ttcactaaag
     2881 cctcaatacc agccagacaa ctagggggta aattacctgc acccaatgcc tcaatcacaa
     2941 caccatcgca tttgcttttc gctaattgtt ccagcagaaa actatccatt ccggcgtatg
     3001 ctttcagtag ttgcacgtta gctttagtta cttgttgaat tggataacgt tcgtaagtgg
     3061 tcaatttttg gaaaaaaatg acagattctt tcgttactaa gccacaaggc ccaaaggttg
     3121 gggtttggaa agtcgccaca ttagtggtgt gggttttggt cacaaattta gcattgtgga
     3181 tttcatcatt catcaccact aacacgcctt tatctcggct ttcttcattt aacgccacta
     3241 aaatcgcact ttgtagattg attaagccat cagcacccag ttcgttactg gaacgcattg
     3301 ctccggtaat tactaccggt atatttacgc tgagggctaa atccagaaaa taggaggttt
     3361 cttccaaggt atctgtgccg tgggtgatta ctatgccatc gacattctcc tcattcactg
     3421 ccttttcaat ccgcattttt aactgttgcc aatgctcgat attgatatgc ggagagggaa
     3481 tattaaataa cgattcttgc accaattttg ctgggtggtt taaacgttct aaggcagcca
     3541 agagaggatt ttcattggaa ggggagacct taccgtcttc cccttctttc attgaaatag
     3601 taccaccagt gtggaggatt aataattttt tagacatctt ttatacaata gttattcaac
     3661 acgaggatca ggattatcta ataccatctg agtttgggtt tgacggaaaa tacgcagttt
     3721 ctctgcaata tcagggttaa aggctgctaa aatttgagcg gctaataagc ctgcatttgc
     3781 tgcacctgcc ggcccgattg cgagcgtacc gaccggaata cctttcggca tttgcacaat
     3841 cgaatagagg ctatctacac cacttaacat tgagcttttt accggcacac ctaacaccgg
     3901 cacaatggtt ttagcagcga tcattccggg caagtgagct gcaccgccgg ctccggcaat
     3961 aatcacttta tagccgttag tctctgcagt ttcagcaaaa gaaaagagtt tgtccggtgt
     4021 gcggtgtgct gagacgactt caacgtgata gctcaaaccg aactggtcta atatttgggt
     4081 tgcttcagac attgtagccc aatcactctt agatcccatt acgatcgcaa tttcagcttt
     4141 atttggcatt ttaaatcctt atgatactaa tgtaaaacag tgtaatcata gcatacaaag
     4201 caaacgtttg cgtagctatt taagaagtga aaagcaattt ttagggctgt tatctgctgg
     4261 ttaaaatatg atataatcga caaccatttt attttttata aatttaatca tcggagatat
     4321 aaatgacgag tagtataaac aaacgttcgg taatgactct tttttcggat aaaaacgata
     4381 tttacagcca ccaagtgcgt attgtgttag cagaaaaagg ggtgccttat gaaatagaga
     4441 acgttatgct aggcagtatt tctgaagatt tattagaatt gaatccttac ggtaatattc
     4501 ctacattggt tgatcgtgaa ttagtgctgt ttaaccctcg tattattatg gaatatttag
     4561 atgagcgttt ccctcaccca ccattaatgc cggtttaccc tgtttctcgt ggtcagtgcc
     4621 gtttaaatat gtatcgtatt gaacaagatt ggtattcgca aattgatatt gtgaataaaa
     4681 atcctgaatc agcggaaggc aagaaagcgt tagcacagct taaagaagat gtattggcat
     4741 tagcgcctat ttttgccgct aaaccttatt ttatgagcga tgatttcagc ttggttgatt
     4801 gctatattgc accgttatta tggcgtatgc aaatcttagg cgttaaattc agcggtgcaa
     4861 cggcaaaacc aattaatgcg tatatgaccc gtgtattcca acgtgatagt tttatccagt
     4921 ctgttggcgg cgaagcgcca aaacatttaa tggacgataa agactaatga aaccattaag
     4981 accttatctt tataatgctt actataactg gattattgat agcgataaca cgccttactt
     5041 attagtgaat gcagaatttc cagacgtaga tgtgccggta gagtttgtca aagaagggaa
     5101 gattatttta aatatttcgc ctcgttccat cgggcagtat attgtggctg atgaatacat
     5161 tagctttagc gctcgttttc agggaatgtt aagagatatt tatattcctt ttggggcaat
     5221 ggtggcgatt tatgcgcaag aaaccggtga tggtattatg ttccaagaag aagcctacta
     5281 cactgaagag gcttatcaag ctcgagttag agcagtagag cagcctaaaa aaatcaaggc
     5341 gaagaaaaca tctaccttaa agttggtgaa ataagagatc tatttcatca aaacacatta
     5401 caagcggtta aatttttctg attatttgca aatatcagtc aaaatttaac cgcttgtttg
     5461 ttttgtgacg gaaataatca aattccactt gcactccgac taaaaattgc ttatctttgt
     5521 tttgtgtttg tactgttgtg atagtacagt aatgatctta aggagagaaa atgaaattcc
     5581 ataaaacctt attagtgact ttgttaagtt cactttctgt agcggcatta gctcaagata
     5641 aaattcaaaa tgtagcaatt acagcgattg tggaacaccc tgcattagat tcaattcgta
     5701 aaggtgtgat tgaagaatta gcccgagaag ggtttgttga tggtaaaaac attaaaatcg
     5761 actatcagtc tgcacagggt agtactgcaa cagcagcaca aattgcgcgt aaatttgttg
     5821 gggataaagc agatattatt atcccgatca ccacaccttc ggcacaacct gttgttgctg
     5881 caacacgttc tatcccaatt gtgttttcag gcgtgacaga cccggttgct gcaaaattag
     5941 taaaatcgtg ggagccaagt ggcactaacg tgacggggat ttcagaccac aaagcgatgg
     6001 ctccacaagt gaaattaatt caaacattag taccggattt gaaagctgtt ggttatgtgt
     6061 atagtgcagg tgaagttaat tctgcgattg tgttagaaga gttaaaacaa gaggctcaaa
     6121 aacagggctt tactatagtg ccggttgctg ttcaacgcag tgccgatatt ggcactgctg
     6181 ctcgcagcct aaacggaaaa gtgcaggcta tttatatttc tgaagataat gcagttgttt
     6241 cggcttatga agccttacat aaagcggctt tagaagcaaa aattccggtg attgctgctg
     6301 accgtgatac agtacaacgt ggtgcagtgg cagcttatgc ggttaatcaa tatgatattg
     6361 gtgtggcaac cggtaaagtt gctgctcgtg tattaaaagg ggaaaaagca ggtacaattc
     6421 caatccaaga agtcagcaaa ttagagcttt caattaatac taaaacggca aaagaattgg
     6481 gcattactat accagaggta ttaatcaaag aagctaaaca aactttttag tgtaaaaaat
     6541 aaaaatttgt tttatttgtc ataatcttgt agagtaaaaa ttcatttcaa ctcagctaag
     6601 gagaatatta tgggatggat tgctgcaatt attgttggcg ctataattgg ttggatagct
     6661 tcggctgtaa tgaaaagtga aggcggactt tgcaaaaata ttattattgg tattgtgggt
     6721 tcgtcacttg gacgctgggt atttggtgat gtattaggta ttggttccgc ttatagtgcc
     6781 ggagacttta atatagtcgg tatctttttt ggtgtattag gggcagctct tttgattttc
     6841 attttgagaa aattaagcat aattaactaa tttcgtattt cagaaaggaa gcagttgttt
     6901 tattactgct tcttttttgt taaatatact ctttttgctc ttgcatcagt tgtgtgaaaa
     6961 aatcaattag gattcgcact ttaggagaga gataacgacg gtttggatag agtaagctga
     7021 tttgtagagg ttcctgtgtc aaaaaaggca taataggaat caactctccg ttttttagct
     7081 cgtgttttat taaaaacttt ggaatgttaa tgatgcccaa accagctttt gccatttgta
     7141 ctaacgcata accattgtta ctgtaaattt taccttgtgg tttaatttgc attttttgtc
     7201 cttgttgaga aaattgccat tgaggatggt attcatagag taggcagcct cgttccacta
     7261 atgcttctaa gcctttaggt tcgccaaatc gagctaaata atctggagaa gcaactaaaa
     7321 ccaaatggga atctgcaatt tttttcgcta caatggaact atctgcaagg ctaccaatac
     7381 gcagcaccaa atcaaagcct tctgcaatca catctacccg acggtcacta aattcaatat
     7441 gaagggctaa attagggtaa tcttccataa atttcccaag gtaaggggca atgaagcgag
     7501 agccaaaatc cattggtaca gacattgtta ttttcccttg tagcgttgag ttaatattac
     7561 ttacgctggc atcggcctct tttagttcat ttaaaatagg caagcattgc tgatagtaga
     7621 gcaaccctgc ctcagttggc gtaatttttc gagtggttcg ttgtagtaaa cgcacattga
     7681 gatgttcttc taactgagcc accaatttac ttgccatggg taacgaaatt tgagcttgtt
     7741 gtgctgcttg agtgaaactt tgggtttcaa ttactttaca aaataaactg attgcgttta
     7801 gtttatccat tttctctctt ctttatattg taatttgtgt taaggcttgc tatcgtaaaa
     7861 atagtgtata aaaaaatcaa cttaaaaaga aataatttca aacaatttaa aataattatt
     7921 aaaaattagg taataaatta tgggaaaatc actagttatt gtggagtctc cggctaaggc
     7981 aaaaaaaatt aaccaatatc ttggtagtaa ttatgtggtg aaatcgagtg tggggcatat
     8041 tcgtgattta ccgaaaagtg gtagtgatga aaaaacggaa aaagcgaaac cgatttcaac
     8101 caaagggctg accgctgccg aaaaagctaa aatcaaagca gaaaaagagc gtgttgcatt
     8161 ggttaaacgt atgggtatcg acccctataa tggctggaaa gctaactatc agattatgcc
     8221 ggataaagag aaagtggttt ccgagctaaa atcgttggca aagaaagcag atcatatcta
     8281 tctggcaacc gacttggata gagaagggga ggcgattgcg tggcatttgc gtgaaatcat
     8341 tggtggtgat gacagccgtt ttagccgagt ggtatttaat gaaatcacca aagatgcgat
     8401 caaaaaggct tttgacgatc cgcaaccgct caatattgac cgtgtaaatt cccagcaaac
     8461 ccgccgcttt ttagaccgtg tagtgggctt tatggtgtcg ccattattat ggaaaaaagt
     8521 agctcgtggt ttatcggcag ggcgtgtgca atcggttgcc gtaaaattac tggttgagcg
     8581 tgagcgtgaa atcaaggcgt tccaaagcga ggagttttgg actattcttg ccaatacgca
     8641 aattaatcgt aaaaaattac cgcttgaatt aactacttat aaaggtaaaa aattctcggc
     8701 aagcaatgag gctgaagcaa atgttgcagt gcgtgctttg gagcagagcg attttattgt
     8761 ttctaatatt gaagccaaag aaaccagctc tcgagctgct gcaccattta ttacctcatc
     8821 gttacaacgt gccgctagca ctcgtttagg ttttggggtt aaaaagacga tgatgttagc
     8881 ccaacggtta tatgaagcgg gttacatcac ctatatgcgt actgattcaa ccaaccttag
     8941 ccgtgaagca ttagcaatgg ctcgaggtta tattcaggat aatttcgggg cgaattattt
     9001 gccggaaaaa gcaaatgtgt atcgcagtaa agataatgcc caagaagctc acgaagccat
     9061 tcgcccgtcg gatgtgaatg tcaaaatggc ggatttaaaa ggaatggata aagatgccga
     9121 gcgtttatac gatttaattt ggcgtcgttt cttggcgtgt caaatgttgc ctgctcgtta
     9181 tgattctacc gcagtcacag tgtctgccgg agactatgaa ctcaaagcaa acgggcgtgt
     9241 actgcgtttt gacggctgga cgaaagtact gccggtgcaa ggcaaaaatc cggaagatca
     9301 ggaattaccg gcggttgaac tgcatcaaaa attaacgctt gatgaaattg tgccaagtca
     9361 gcattttact aaaccgcctg cacgttttaa tgaggcgtct ttggttgctg agttagaaga
     9421 gcgaaaaatt ggccgccctt caacctatgc ttcaattatt tcgacgattc aagaacgtgg
     9481 ctatgttcgc accgaaaaca agcgttttta tgtggaaaaa atcggtgaag tagtgacgga
     9541 tcgcctcaac gaatcattca aagatttaat gaattatgat ttcactgcca atatggaaga
     9601 ggtgctcgac caaattgcag aggggtctaa aaattggaaa gaagagttga atactttctt
     9661 taaacatttc tctgaagagt tgagccaagc agaattagat gaacttgaag gcggaatgaa
     9721 accaaacagc cttgtgccaa ccgatattga ttgcccgact tgtggacgaa aaatggcaat
     9781 tcgcaccgct tccacagggg tatttttagg ttgcaccggc tatgctttac cgccaaaaga
     9841 gcgttgcaaa acgacaatga atttaattcc agaggcggaa tttttcaatg tgttagattc
     9901 ggattctgaa accaaagccc taatggctcg caagcgttgc ccgaaatgcg attcggcaat
     9961 ggatagttat gtgattgatg ctgaacgtaa attacacgtt tgtggtaata atccaaattg
    10021 tgatggtcat attattgagc aaggttcctt caaaattaaa ggctatgacg gacctgtagt
    10081 ggaatgcgat aaatgcggtt cagatatgca cttaaaactg ggtcgtttcg gcaaatatat
    10141 gggctgcaca agctgcgata acacccgtaa aattctgaaa aacggggaag ttgctccgcc
    10201 aaaagaagat cctattgctt ttcctgaatt aaaatgtgaa aaagccgatg cctattttgt
    10261 attaagagac ggagcaagtg gggtgtttat gtcagctcat aacttcccga aagtgcgtga
    10321 aagccgtgca ccaaaagtgg tagagttagc tcaatatcgt gaacgtttgc cggagaaatt
    10381 gcagtatctt gccgatgctc cgcagcaaga tccggaagga
//
LOCUS       DANKBV010000074        10348 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.074, whole genome shotgun sequence.
ACCESSION   DANKBV010000074 DANKBV010000000
VERSION     DANKBV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10348
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.074"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1376
                     /gene="radA"
                     /locus_tag="PWY43_001575"
     CDS             <1..1376
                     /gene="radA"
                     /locus_tag="PWY43_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL2136443.1"
                     /translation="KAPKTAYVCNDCGAEYSRWMGQCRECKSWNTISEVRLISSKETA
                     KSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGFNELDRVLGGGVVPGSAILIGGHPG
                     AGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANRLGLPTANLKMLSETSVEHIC
                     NIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVGHV
                     TKDGTLAGPKVLEHAIDASLLLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGLKE
                     VKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHSMLANPRRVAVGLDHNR
                     LSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQDLV
                     IFGEVGLAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLSDA
                     LDILYNL"
     gene            complement(1431..1661)
                     /locus_tag="PWY43_001576"
     CDS             complement(1431..1661)
                     /locus_tag="PWY43_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDL2136444.1"
                     /translation="MDYVEILGYVAMILVACSFLLKDVIKLRVVNSLGCACFVVYGLL
                     IGSIPVTGLNALVVCINLYYISKGNRPTATHS"
     gene            complement(1781..2275)
                     /gene="folA"
                     /locus_tag="PWY43_001577"
     CDS             complement(1781..2275)
                     /gene="folA"
                     /locus_tag="PWY43_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDL2136445.1"
                     /translation="MKISIIVARTINKVIGKDNQMPWHLPVDLAWFRQNTLGKPVIMG
                     RKTYESIGRLLPKRPNIILSRSGFTVEGAYAVQSLEQAVELAKNFANTAEIMIIGGGE
                     LFKQALPLAERLYLTEIQADIEGDTFFEFDEQNWALVEEKWSEIDENNPFRCRFMCLE
                     RKSV"
     gene            2387..3478
                     /gene="proB"
                     /locus_tag="PWY43_001578"
     CDS             2387..3478
                     /gene="proB"
                     /locus_tag="PWY43_001578"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL2136446.1"
                     /translation="MSKTIVIKFGTSTLTHGTKSLSRPYMLELVKQIAQLHQQHRVIV
                     VTSGAAAAGRDYLGHPELPKTLASKQMLAAVGQSQLIRVWENLFDIYNIHIGQMLLTR
                     ADLDDRERFLNARDTLDALLAQKIIPVINENDAVATEEFKVGDNDNLSALVAILAQAD
                     QLYLLTDQEGLFSADPRSNPDAKLLSVVEKITPEIRQIAGGSSTGLGTGGMSTKITAA
                     DIATRSGVETIIASGSRPNVLVDLANGQNIGSKFLVQSDRLEGRKRWLFAAPQVGEIV
                     VDFGAEQALLQHKSLLPVGVSAIKGEFTRGEVIKIFSQDGKALALGIARYSSEALLRI
                     KGKKSKEIESLLGYEFGSVAVHSDEMVVY"
     gene            3478..4242
                     /locus_tag="PWY43_001579"
     CDS             3478..4242
                     /locus_tag="PWY43_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDL2136447.1"
                     /translation="MELGIDVLAILFAVATLAGFIDAIAGGGGLLTIPALMAAGLPPA
                     MALGTNKLQACGGSLSSSLYFIRKKAVNLKQIWLLILCTFLGSAAGTIFVQMINVDFL
                     KTLLPFLILIIGIYFLVSPKIGDEDRKQRISFTLFAFTAAVALGFYDGMFGPATGSFM
                     TLAFILLLGFNLSKAVAHAKVINFTSNFASLIFFTLGGAVMWKIGLIMMVGQFIGGTL
                     GAKMVMTKGKKLIRPMLVTMSFMMVGKMLYEQGIFN"
     gene            4245..4877
                     /gene="nth"
                     /locus_tag="PWY43_001580"
     CDS             4245..4877
                     /gene="nth"
                     /locus_tag="PWY43_001580"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL2136448.1"
                     /translation="MNKAKRIEILTRLRNENPKPTTELNYSNPFELLIAVILSAQATD
                     KGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNG
                     EVPEDRDALEALAGVGRKTANVVMNTAFGHPTIAVDTHIFRVSNRTNFAPGKNVVQVE
                     EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEFKEKTE"
     gene            5072..6322
                     /locus_tag="PWY43_001581"
     CDS             5072..6322
                     /locus_tag="PWY43_001581"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HDL2136449.1"
                     /translation="MDIYLVGGAVRDQLLGLPVKDRDFLVVGATAEQLLAQGFQQVGA
                     DFPVFLHPETKEEYALARQERKNGNGYNGFICDFSPNVTLEQDLIRRDLTINAIAQDE
                     NSTLFDPYGGVQDLENRLLRHVSPAFSEDPLRVLRVARFAARFHSFGFTIAPETLKLM
                     REMVQSGELEHLTAERVWLETQKAFETDNPHVYFDILRLIGALKVLFPELDRLFGVPQ
                     PPQHHPEIDSGKHTLMVLQQAKRLAKKAENPTALLFAALCHDLGKGLTPADILPHHYG
                     HEVKGIQPTRELANRLKIPTEIKDFALLVTEYHTHCHKMAELRPETVIKLFNSLDVWR
                     KPNRFFDFLFACEADARGRLGFEQRNYPQPELAKKYYQAAIEIDVQQVIQDGFEKQAI
                     REELNKRRVYAVRSIKLDSDLINH"
     gene            complement(6423..7358)
                     /locus_tag="PWY43_001582"
     CDS             complement(6423..7358)
                     /locus_tag="PWY43_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL2136450.1"
                     /translation="MSESKPFPITTGFFAMPLGLGALGLAWLNATPITPYATTIGNII
                     AGTSVILWVALLGCYVYRVFFCFEQIKAEFMHPVQCCLFSLVTMTTMISGDVLYVWGF
                     YLGELLIFLGVMVHLIFLVFIIGGLWKGTQFLPETLHPVHYLPPIAGSFTMASSLSLI
                     GYSEIASLFLGIGIISWLVYEPTMLQRIRLSPLFPIARPSLGIVLAPAFVGASAYFSM
                     GGELDLLAKMLWGYGFLQFCFILRNLKWIAENGFSMGFWAFSFGLAAMARGAIEFYQF
                     EPLSFIGLAGFTIANFGIGLLLIFTLKLWLQQSRA"
     gene            complement(7487..7681)
                     /gene="iscX"
                     /locus_tag="PWY43_001583"
     CDS             complement(7487..7681)
                     /gene="iscX"
                     /locus_tag="PWY43_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDL2136451.1"
                     /translation="MKWTDTRMIAENLYDMNPDLDPTTVRFTDMHKWICEMEDFDDDP
                     EASNEHILEAILTIWLEEYE"
     gene            complement(7681..8022)
                     /gene="fdx"
                     /locus_tag="PWY43_001584"
     CDS             complement(7681..8022)
                     /gene="fdx"
                     /locus_tag="PWY43_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDL2136452.1"
                     /translation="MPKIVFLPHEEFCPEGMVVEAKAGDNLLEVAHEAGVEIHHACDC
                     SCACTTCHVVIREGFDSLNDTSDQEEDMLDKAWGLEIDSRLSCQCIVGEEDLVVEIPK
                     YNLNHANEAAH"
     gene            complement(8041..8406)
                     /locus_tag="PWY43_001585"
     CDS             complement(8041..8406)
                     /locus_tag="PWY43_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 51 family protein"
                     /protein_id="HDL2136453.1"
                     /translation="MSNYVKTEISEQFRTVAVEFDLEDNRIFELGERINTEYDVAYMN
                     GYNWAAFLRAYLSIYRPSLLELLEEDPDVGCYYVTYPLSEKSKEKARELKKIIVYLVE
                     NEEEILNFIGEHLDNIEWD"
     gene            complement(8416..10269)
                     /gene="hscA"
                     /locus_tag="PWY43_001586"
     CDS             complement(8416..10269)
                     /gene="hscA"
                     /locus_tag="PWY43_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDL2136454.1"
                     /translation="MALLQISEPGQTAAPHQHKLAVGIDLGTTNSLVATVRSGQTQVL
                     LDEQERALVPSVVHYAETEKTVGIEAFAKAALDPKNTVISAKRLIGRSLADVQQRYQN
                     LPYQFVASENGLPLIQTAQGNKSPIEVSADILSHLAQFAEKRLAGELSGVVITVPAYF
                     DDAQRQSTKDAARLAGLNVLRLLNEPTAAAIAYGLESGQEGVIAVYDLGGGTFDLSIL
                     RLRKGVFEVLATAGDTALGGDDFDHLLAEWIAEQANYKPQTASEQRELLTLATQTKVA
                     LSQAVEFEVKFANWTGVVSRSQFNELIQPLVKRSLMTCRRALKDAGVEAEEVREVVMV
                     GGSTRVPFVREQVGEFFGKTPLTSIDPDKVVAIGAAVQADILVGNKPDSDMLLLDVVP
                     LSLGIETMGGLVEKIIPRNTTIPVARAQEFTTGKDGQTAMTIHVLQGERELVEDCRSL
                     GKITLRGIPAMAAGVAQVRVTYQVDADGLLNVTAMEKSTKVQASIQIKPSYGLTDEEV
                     TQMIKSSMANAKEDMEARQLVEQRVEADRVIDSVVTALKEDGSAVLSVEEFQAIEAQL
                     ARLIELKEGTARFAIQQGIKEVDLATQEFAARRMNLSIQKALAGKKMDDLA"
BASE COUNT         2982 a         2317 c         2107 g         2942 t
ORIGIN      
        1 caaaagcacc taaaaccgct tatgtttgta atgattgcgg ggctgaatat tcccgttgga
       61 tggggcaatg tcgggaatgt aagtcttgga ataccatcag tgaagtgcgg ttgatttcca
      121 gcaaagaaac cgccaaatct gaccgcttta gcggctatgc aggtgaaact tcaggcaaag
      181 tacaaacgct gtctgacatc agcttgcagg aagtgcctcg ttttagcagt ggctttaacg
      241 agctggatcg tgtactcggt ggcggtgtgg taccggggtc agcaatactg attggtggcc
      301 acccgggggc agggaaaagt acgttattac tgcaagtaat gtgcggttta gcccaacatc
      361 taccaacgct ttatgttacc ggtgaagagt cgttacaaca agttgcaatg cgggcaaatc
      421 gcttaggctt accaacggca aatctgaaaa tgttgtctga aacctctgtc gagcatattt
      481 gcaatattgc agaccaagaa aaaccgaaga ttatggtgat tgactcgatt caagtgatgc
      541 acttgtcgga tattcaatcc tcaccgggca gtgtttcaca agtgcgtgaa tgtgcctctt
      601 tccttacccg ctacgccaaa acccgccaag tggcgattat tatggttggg cacgtcacta
      661 aagatggcac gcttgccggc ccgaaagtgt tggaacacgc cattgatgct tccttgctgt
      721 tagaaggcga atccgattcc cgtttccgta ctttgcgtag ccagaaaaac cgctttggtg
      781 ccgttaatga attgggcgta tttgcgatga ccgagcaagg gctaaaagaa gtgaaaaacc
      841 catctgcgat ttttttaagc cgtagcgaag agcaaacgcc gggcagttcg gtgatggtgt
      901 tatgggaagg aacacgccca ttgttggttg aaattcaagc cttagtcgat cactcaatgc
      961 tggcaaaccc tcgccgtgtg gcggttggct tagatcataa ccgtttatcg ctattacttg
     1021 cggtgctaca ccgacacggt ggcttgcaga tgtcggatca agatgtgttt gtaaatgtgg
     1081 tcggtggggt gaaagtcacg gaaaccagtg ccgatttagc cttaattttg gcgttaattt
     1141 ccagcttccg taaccgtcca ttgccgcaag atttggtgat ttttggggaa gtcggcttag
     1201 caggggaaat ccgcccggtt accagtgggc aagagcggat cagcgaagcc gcaaaacacg
     1261 gctttaaacg ggcgataatt cctcacggca atgtcccgaa aaaggcaatt aaagatatgc
     1321 aggtgttcac cgtgaaaaaa ctcagtgatg cgttagatat tctgtataat ctctaaaatc
     1381 aagaaccgct tgtaagtctg tcttacaagc ggtctgtttt aggtaaaaat ttacgaatgt
     1441 gtggcagttg gtctattgcc tttggaaata tagtataaat taatacacac caccaacgca
     1501 tttaagccgg tgaccggaat cgagccaatc agcaaaccat aaacaacgaa acaggcacaa
     1561 cccagcgaat tcaccacccg cagtttaatc acatctttta ataaaaaaga acaggcaact
     1621 aaaatcatcg ccacatagcc gagtatttca acatagtcca tttagaacct cctcaaataa
     1681 aataaactgc cgatatttta aaggctgttt ttttatttgt atctagctct aaaaatagca
     1741 aaggtttgcg ttcacatttt tacttttaac cgcttagtta ttaaacagat tttcgttcta
     1801 aacacataaa acggcaacgg aaagggttat tttcatcaat ttctgaccac ttttcttcta
     1861 ctaacgccca attttgttca tcaaattcaa agaacgtatc cccttcaatg tctgcttgaa
     1921 tctcagttaa ataaagtctt tctgctagtg gtaaggcttg cttaaacagt tcccccccac
     1981 caataatcat tatttcagct gtatttgcaa aatttttcgc taattcaacc gcttgttcga
     2041 gcgattgtac cgcataagcc ccttccaccg taaaacctga acgggaaaga ataatattag
     2101 ggcgttttgg caataacctg ccgatagatt cataggtttt ccgccccata atcaccggtt
     2161 tgccgagcgt gttttggcga aaccaggcta aatctaccgg caaatgccac ggcatttggt
     2221 tatctttgcc aatcaccttg ttgattgttc gggcaacaat aatgctgatt ttcatttttt
     2281 ctcctgttat gcaaattggt ttgattttat atcaaattaa gcgtgatttt acaccctaaa
     2341 agtgatactt ttggcaacat tatttttagt tacaagtgaa cagattatgt caaaaactat
     2401 cgtgataaaa tttggcacca gcactctcac acacggcact aaaagtttaa gccgtcctta
     2461 tatgttagag ctggtaaaac aaatcgctca attacaccaa cagcatcgtg tgattgtggt
     2521 gacatccgga gctgccgctg ccggtcggga ctatttagga caccctgaac tccctaaaac
     2581 cttagcctct aagcaaatgt tagcagcggt cgggcaaagt cagctcattc gagtgtggga
     2641 aaatttattc gatatttata atatccatat cgggcagatg ctcttaaccc gagccgatct
     2701 ggacgatcgt gaacgtttcc tcaatgcccg tgataccctt gatgccctgc ttgcacaaaa
     2761 aatcattccg gtgattaatg aaaacgatgc cgttgctacc gaagaattta aagtaggcga
     2821 taacgataat ttatcggctt tagttgcgat tttggctcaa gcagatcagc tctatttatt
     2881 aaccgaccaa gaaggcttat tcagtgccga tcctcgctca aaccctgatg ctaaattgct
     2941 ctcggtggtg gaaaaaatca cgcctgaaat ccgccaaatt gccggtggta gttcaaccgg
     3001 tttaggcacg ggcggaatga gtaccaaaat tactgctgct gatattgcta cccgctccgg
     3061 tgtggaaacc atcatcgcct ccggttctcg cccaaatgtg ttagttgatt tagcaaacgg
     3121 gcaaaacatc ggcagtaaat ttttagtcca atctgaccgc ttggaaggca gaaaacgctg
     3181 gttatttgct gctccgcaag tgggtgaaat tgtggtagat ttcggggcgg aacaagcctt
     3241 gcttcaacat aaatcattac tgccggtggg agtttctgcc atcaaaggcg aatttactcg
     3301 aggtgaagta atcaaaattt tcagccaaga cggcaaagcc ttagcactcg gcattgcccg
     3361 ttatagcagt gaggcattgc tgcgaattaa aggtaagaaa tcaaaagaaa ttgaatcgct
     3421 ccttggttat gaatttggtt ctgttgcagt gcatagtgac gaaatggtgg tgtattaatg
     3481 gaattgggaa ttgatgtttt agcaatcttg tttgccgttg cgacccttgc cggctttatc
     3541 gatgccatcg ctggcggcgg tggcttactc actattccgg cattaatggc agcagggctt
     3601 cctccggcaa tggcacttgg cacgaataaa ttacaggcgt gtggcggatc actctcttcc
     3661 tcactctatt ttattcgcaa aaaagctgtt aatttaaaac aaatttggtt gctaattcta
     3721 tgcacttttt taggctctgc cgccggtact atttttgtgc aaatgatcaa tgtcgatttc
     3781 ttaaaaacat tgttgccttt cttaattctg attatcggca tctatttttt ggtcagccct
     3841 aaaattggcg atgaagatcg taaacaacgc attagcttta ccctatttgc ctttaccgcc
     3901 gctgtcgctc tcggttttta tgatggaatg ttcggaccgg caaccggctc ttttatgact
     3961 ctggcattta ttttgctgct cggcttcaac ctctctaaag ccgtagcaca cgccaaagtg
     4021 attaatttta cctccaactt tgcctcatta attttcttta cgcttggtgg tgcggtaatg
     4081 tggaaaatcg gcttaattat gatggtcggg caatttattg gcggcacact aggtgcgaaa
     4141 atggtgatga caaaaggcaa aaaattaatc cgtccgatgc tagtgactat gtcttttatg
     4201 atggtcggca aaatgctcta cgagcaagga atttttaatt aacaatgaat aaagcaaaac
     4261 gtattgagat tttaacccgt ttacgcaatg aaaatccaaa accgacaact gaactcaatt
     4321 acagtaatcc gtttgaatta ttaattgccg tgattctatc cgcccaagcg accgataaag
     4381 gcgtaaataa agccaccgat aagctcttcc ctgtcgccaa cacgccacaa gcgattttag
     4441 atttaggcgt agatgggcta aaagagtaca tcaaaactat cggcttattt aacagcaagg
     4501 cagaaaatat tatcaagacc tgccgagatc tgatcgaaaa acataacggc gaagtgcctg
     4561 aagacagaga tgccctcgaa gcccttgccg gtgttggcag aaaaaccgcc aatgtggtga
     4621 tgaataccgc attcggacac cctacgattg cagtcgatac ccatattttc agggtgtcga
     4681 accgcacgaa cttcgctccc ggcaaaaatg tagttcaggt agaagaaaaa ctgctcaaag
     4741 ttgtgccgga cgaatttaaa gtcgatgttc accattggtt gattttacac ggacgctaca
     4801 cttgcattgc ccgtaagcct cgttgtggtt cttgtattat tgaagattta tgtgagttta
     4861 aagagaagac ggaataacat tatctggtgg tatttaatga ataactcccc ttttagaaaa
     4921 gtaggggctg ggggagattt gatactattt caacatttcg tatgagcgta tagaaattct
     4981 gccaaatctt ccctaccccc tctttgctaa agaggggaac tagatcgagc gtaaaatata
     5041 acaagcggtc attttttgag attttttgca aatggatatt tatttagtcg gtggagcagt
     5101 gcgggatcaa cttcttgggc tgccagtgaa agatagagat tttttagtgg ttggcgcaac
     5161 tgccgaacaa cttttagccc aaggatttca acaagttggg gcagattttc cggtgttttt
     5221 acaccctgaa accaaagaag aatatgccct tgctcgccaa gaacgcaaga acggcaatgg
     5281 ttacaacggc tttatttgcg atttctcccc aaacgtcacg cttgagcaag atttgatccg
     5341 ccgagattta accattaacg ccatcgccca agatgaaaac agcacacttt ttgatcctta
     5401 tggcggtgtg caagatttag aaaatcggct actccgccac gtttctcctg cattcagcga
     5461 agatccgcta cgggtgctac gggttgcccg ctttgccgcc cgttttcatt catttggttt
     5521 taccattgct cctgaaaccc tgaaattaat gcgagagatg gtacaaagcg gtgaattgga
     5581 acatttaacc gctgaacgtg tgtggttgga aacacagaaa gcctttgaaa ccgataatcc
     5641 gcacgtttat tttgacattt tacgcttaat tggggcatta aaagtgctat tccctgaact
     5701 ggatcgtctg tttggcgtac cacaaccacc tcaacaccac cctgaaattg actccggcaa
     5761 acacactcta atggtgctac aacaggcaaa aagacttgca aaaaaagcgg aaaatccgac
     5821 cgctttactc tttgcagccc tttgccacga cttaggcaaa ggcttaacgc ctgctgatat
     5881 tttgcctcat cactacggac acgaagtcaa aggcattcaa ccaacccgag agttagccaa
     5941 tcggttaaaa atcccaactg agattaaaga ttttgccctt ttagtgaccg aataccacac
     6001 gcattgccat aaaatggcgg aacttcgccc tgaaacggta ataaaactgt ttaacagcct
     6061 agatgtgtgg cgtaaaccaa accgcttttt tgatttcctg ttcgcctgtg aagcggatgc
     6121 tagagggcga ttaggttttg aacagaggaa ttacccccaa cctgaattgg cgaaaaaata
     6181 ttaccaagcc gcaatcgaaa ttgatgtgca gcaagttatt caagacggtt ttgaaaaaca
     6241 agcaattcgg gaagagctga ataaacgcag agtgtatgcg gtaagaagca tcaagctaga
     6301 tagcgaccta ataaaccatt aggttatccg ccactttctc aaaatcaatt cccaaagttt
     6361 aagttggtta aatgtatgga ttctttaaaa ggtggcgtta ttttagtgaa tttataatgt
     6421 tcctatgctc ttgattgttg tagccacaat ttcagggtaa agatcagcaa taacccaatg
     6481 ccgaaattgg caatagtgaa gccggctaac ccaataaaag ataaaggttc aaattgataa
     6541 aattcaatcg cacctcttgc cattgcagct aaaccaaatg aaaatgccca gaaacccatc
     6601 gagaagccat tttcagctat ccatttgagg tttcgtaaaa taaaacagaa ttgtaagaat
     6661 ccatatcccc ataacatttt tgccaacaag tctaattctc cacccataga aaaataggca
     6721 gaagcgccca caaatgccgg agcaagaaca attcctaaag atggtctagc aatcggaaaa
     6781 agcggactaa gccgaattct ttgtaacata gtgggttcat atactaacca ggaaattatg
     6841 ccaatgccta aaaataaaga ggcaatttca ctgtagccaa ttaagcttaa tgaacttgcc
     6901 atagtgaagc tccctgcaat tggcggtagg taatgtaccg ggtgtagcgt ttccggcaaa
     6961 aattgtgtgc ctttccataa tccaccaata ataaaaacca agaaaattaa atgcaccatg
     7021 acgcctaaaa aaatcaatag ttcgcctaaa taaaaacccc atacatagag cacatcaccg
     7081 cttatcatgg ttgtcattgt cactaaggaa aataaacaac attgcactgg gtgcataaat
     7141 tctgctttaa tctgctcaaa acaaaaaaac acacgataca cataacagcc taataaggct
     7201 acccaaagta tgactgacgt tccagcaatg atattaccaa ttgttgtagc ataaggagtg
     7261 ataggcgttg catttaacca ggcaaggcct aacgccccca gccctaatgg cattgcaaaa
     7321 aagcctgtag ttattggaaa aggtttagat tcactcataa atttatcctc atattgaaat
     7381 aatgcataac tgtattcggc tttataacag tcatatgtaa aaaattttat ttttctaacc
     7441 gcttgtaata agaaaaacct aaagcacttg ctttaggttc ctactactat tcatactctt
     7501 ctagccagat agttaaaatc gcttcaagaa tatgttcatt tgatgcttcc ggatcatcat
     7561 cgaaatcttc catttcacaa atccatttat gcatatctgt aaaacgaacc gtggtcgggt
     7621 ctaaatccgg gttcatatca tacagatttt cagcgatcat tcgggtatct gtccatttca
     7681 ttagtgcgct gcctcatttg cgtgatttaa attatatttt ggaatttcaa caaccaaatc
     7741 ttcttcacca acaatacatt ggcaacttaa acggctgtct atttccaatc cccaagcttt
     7801 atccagcata tcttcttctt gatctgaagt atcatttaac gaatcaaaac cctcacgaat
     7861 taccacgtgg caagtggtac aagcacaaga acagtcgcaa gcgtgatgaa tttcaacgcc
     7921 tgcttcgtgt gctacttcta ataaattatc tccggcttta gcttcaacaa ccataccttc
     7981 cgggcaaaat tcttcgtgtg gaagaaaaac aatttttggc attttaaagt ttccttatta
     8041 ctaatcccat tcaatattat ccagatgttc gccgatgaag tttaaaatct cttcctcatt
     8101 ttcaaccaga taaacaataa tcttctttaa ttcccttgct ttctctttac ttttctcgga
     8161 aagcggatag gtaacataat aacatcctac gtccggatct tcttctaata actctaatag
     8221 gcttggacga taaatactaa gataagcccg taaaaatgca gcccagttat aaccgttcat
     8281 ataggcaaca tcatattcag tattaatccg ctcgcccaac tcaaaaatgc ggttatcctc
     8341 taaatcgaat tcaaccgcaa cggttctgaa ttgttctgaa atctcggttt ttacatagtt
     8401 agacatactg cctccttatg ctaaatcgtc cattttttta ccggctaacg ctttttgaat
     8461 tgataaattc atacgccgtg ctgcaaattc ttgcgtagct aaatccacct ctttgatacc
     8521 ttgctgaatt gcaaagcgag cagtaccttc ttttaattcg attaaacggg ctaattgtgc
     8581 ttcaatcgct tggaactcct ccacactcaa cactgcagag ccatcttctt tcaatgccgt
     8641 taccacgctg tcaatcactc ggtcggcttc tactcgctgt tcaactaact ggcgggcttc
     8701 catatcttct ttggcatttg ccattgagga tttaatcatc tgcgtaactt cttcatcggt
     8761 taagccataa gacggtttga tttgaattga agcctgcact ttggtcgatt tttccattgc
     8821 agtcacgttt aataaaccgt ctgcatcaac ttggtaagtt acccgaacct gtgcaacccc
     8881 tgctgccatt gccggaatgc cacgcaaagt gattttaccg agagagcgac aatcttcgac
     8941 taattcacgc tcaccttgta aaacgtggat tgtcatcgcc gtttgaccgt ctttaccggt
     9001 agtaaactct tgagctcgag ctactgggat agtagtgttg cgtggaataa ttttctccac
     9061 caaaccaccc atcgtttcaa taccaagcga aagtggcaca acatcaagca acaacatatc
     9121 ggaatccggt ttgtttccaa ctaaaatatc cgcctgcact gccgcaccaa tggcaacaac
     9181 tttatccggg tcgatagagg ttaatggcgt tttaccgaaa aactcgccga cttgttcacg
     9241 cacaaacggt acacgggtcg aaccgcctac cattaccact tcacgaacct cttctgcttc
     9301 aactccggca tctttcaagg ctcggcgaca agtcatcagc gagcgtttta caagcggttg
     9361 aattagctca ttaaattgcg aacggcttac tacgcctgtc caatttgcaa atttcacttc
     9421 aaattcaacc gcttgcgaaa gtgcgacttt ggtttgagtt gccaaagtta ataattcacg
     9481 ctgctcgctt gccgtttgag gtttgtaatt tgcctgttca gcaatccatt ccgctaatag
     9541 gtggtcgaaa tcatcgccac cgagtgcggt atcacctgcg gttgctaata cttcaaacac
     9601 acctttgcgt aaacgcaaaa tagataaatc gaaagtaccg ccacctaaat cgtaaacggc
     9661 aatcacacct tcttgtccgc tttctaaacc ataagcaatc gctgctgccg ttggttcatt
     9721 gagcaaacgt aatacattta aacccgcaag acgtgcggca tctttggtac tttggcgttg
     9781 tgcgtcatca aaataagccg gtacggtaat taccacgcct gaaagctcac cggctaaacg
     9841 cttttcggca aattgtgcca agtggcttaa aatatccgcc gaaacttcaa tcgggctttt
     9901 attaccttgc gctgtttgga ttaaaggtaa accgttttcg ctcgcgacaa attgataagg
     9961 taaattttga taacgttgct gaacatctgc cagagaacgc ccaatgagac gtttagctga
    10021 aatgacggta tttttcgggt caagtgcagc tttggcaaag gcttcaatcc caactgtttt
    10081 ttctgtttcc gcataatgca ctactgacgg cactaatgct ctttcttgtt catcaagcaa
    10141 tacctgagtc tgcccgctac gcacagttgc cactaatgaa ttggttgtac ctagatcaat
    10201 tcctaccgct aatttatgtt gatgtggtgc agcggtttga ccgggttcag aaatttgtaa
    10261 taatgccatt tatttatctc tttatctttc taacttagtt ctctattgta ttaaatggag
    10321 tgaaatatat tgtttaagag aagctgga
//
LOCUS       DANKBV010000075        10314 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.075, whole genome shotgun sequence.
ACCESSION   DANKBV010000075 DANKBV010000000
VERSION     DANKBV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.075"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(45..1073)
                     /locus_tag="PWY43_001587"
     CDS             complement(45..1073)
                     /locus_tag="PWY43_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2136455.1"
                     /translation="MTTQFLKNMGMVDDKGKIDFIAFFERFGVLIFLILLIIFFTSQN
                     GAFLSQRNIYNVLTEVSIFGIMAVGMTFVILTAGIDLSVGSILAVTAMFAAYIIKGDN
                     TVTLEAGAWGGMSWLIGLSICLGLGTFIGWLHGLGVTKLKLPPFIITLGGMTIWRGLT
                     LVLNNGSPIAGFDEGYRWWGRGDILGVPVPVVIFAVVAIIGYFALHKTRWGRYVYSVG
                     GNPEAARLAGVNINRTLVSVYVVIGALAGLAGFILSARLGSAESVAGVSFELRVIASV
                     VIGGTSLMGGYGRVTGTIIGSIIMGVLINGLVLMDVSAYYQQIIMGLIIILAVAFDTY
                     AKSRRGAL"
     gene            complement(1085..2629)
                     /locus_tag="PWY43_001588"
     CDS             complement(1085..2629)
                     /locus_tag="PWY43_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2136456.1"
                     /translation="MSTPLLEVKDLTKSFSGVMALNGVKLTVGKGEVHALLGENGAGK
                     STLLKALSGAQPQTSGEIIFNGETLSLSDSPFDRQLKGIVTIYQEFNLLPNMTVAENF
                     FLGREPVKGLFVNEKAVNEEAQLVLDNLGLNIKPDTQVSRLSVAQQQMVEIARAMTLN
                     AKLIIMDEPSAALSDKEVETLHQIVRDLKSRGISVIYVTHRLNEVFALCDRFTVFQDG
                     RYSGEGEVKNVNVDQIIKMMVGREVAFTRRPPEETHHTHRPIRLAVKNLHREKPAFDP
                     HGIALHGVSFNIHEGEVLGIAGLVGAGRTEIARCLFGVENYTADEISLDGKPYAATSP
                     LDALEKGVALVPEDRKKEGLVLGLPIKTNMTLPILSKLLKGNLFVDNQKEDDLIETYR
                     QALRIKMASSELEARKLSGGNQQKVILARCMALNPKVLIVDEPTRGIDVGAKSEVHQV
                     LFDMAKQGVAVLVISSDLPEIMAISDRIITLSEGRVTGEIHGDDATEEKLMSMMAVGV
                     QRKAAA"
     gene            complement(2680..3618)
                     /locus_tag="PWY43_001589"
     CDS             complement(2680..3618)
                     /locus_tag="PWY43_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2136457.1"
                     /translation="MKKRIKFVLSALTLGLAMNSFAKDELVVFSLPNLSSPFEVQLQK
                     VAVETSKKLEIKLQVLDGQSSSTKQASDLENAITRGAKGIIISPNDVNAISGAVEEII
                     KEKIPAATLDRKVESSKPVPHFGANNYTGGQEVAKAVKAKYPNGAKIILLTGQPGSTS
                     NIERTKGIRDELAAGGDKYKIVVDQTGNWLRSEGLRIIESVLPTLKEKPEVIISANDD
                     MALGAIEALRSQGLKAGDILVTGFDSTPEALARVKDGWLYLTADQRPSFAVSTALEQV
                     VGNIRESKEVSGADYPPKIILKDNLQEAERFAEISE"
     gene            complement(3950..5461)
                     /locus_tag="PWY43_001590"
     CDS             complement(3950..5461)
                     /locus_tag="PWY43_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL2136458.1"
                     /translation="MSQFVQNFINGAVVESQSQRVTSVFNPATGEETKKVKLSLPNEV
                     DAAIAVADQAFKSWSQQSPLRRARILFKFKELLEANFDELARLISSEHGKIYSDAIGE
                     LTRGLEVVEFATGIPHLQKGEFSANAGRGIDIHSIQQPLGVVAGITPFNFPAMVPMWM
                     FPVALACGNTFVLKPSEKDPSVSIRLAELLKEAGLPDGVFNVVQGDKEAVDILLTDSR
                     IQAVSFVGSTAIAQYIYEKGSANGKRVQALGGAKNHALIMPDADVQGTINALLGAAFG
                     AAGERCMALSVAVVVGDELADKIIEELIPKVKALKIGSGLLPQGVLENDMGPVISKEH
                     KAKIESYIDQGVAQGAKLCVDGRGYKVEGFENGYFVGGTLFDNVTPEMTIWQDEIFGP
                     VLSVVRVKDYAEGIALINSHQYGNGSAIFTADGDSARQFTQDVQAGMVGVNIPIPVPM
                     AFHCFGGWKASIFGPLNVYGTDGVRFYTRMKTITTRWPDSSVRSQAAFNFPTL"
     gene            5680..6504
                     /gene="iolB"
                     /locus_tag="PWY43_001591"
     CDS             5680..6504
                     /gene="iolB"
                     /locus_tag="PWY43_001591"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL2136459.1"
                     /translation="MSSPLLSKPNFSQPEGLIQHITPENAQWEYVGFDVYQLSKQQIT
                     LESGENELCLVFVTGFGSVYVDDYTFENIGNRTTPFEKIPPYAVYIPPHKSVLIKTEL
                     NLELAVCKAPSQGHYPVRLISPNHIGVEKRGFGNNQRLVHNILPETEPADSLLVVEVF
                     TDEGNTSSYPSHKHDDKHSTEETYLEETYYHRFDPPQGFAMQRVYTDDRTLDECMAVY
                     DKNVVKVPRGYHPVATIAGYNNYYLNVMAGPVRKWKFTWEQDHLWVNSETYKAKFE"
     gene            complement(6580..8031)
                     /locus_tag="PWY43_001592"
     CDS             complement(6580..8031)
                     /locus_tag="PWY43_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL2136460.1"
                     /translation="MPNHKFLLLRFQAYINGKFVSSQNGKTFTVQNPTNGEKIGEVAD
                     LGTEETAQAIEAAAVAQKKWQALLPKERSVILRKWFELIMQNQEELAQILSLEQGKPL
                     AESRGEIAYGASFIEWFAEESKRIYGDVLPQDKAGRRLFVTKQPIGVVAAITPWNFPN
                     AMITRKAAPALAAGCAIVIKPAAETPLSALALAQLADQAGIPKGVFNIITSSDAEGIG
                     RELSTHPTVRKLTFTGSTRVGKILMQQSAGTVKKLALELGGNAPAIVFDDADLDTAVE
                     GVFASKFRNGGQTCVCANRIYVQAGIYEQFLAKLSEKVAQIKLGSPFEEEVTMGALIS
                     ERAVEKVESHINDALSLGAELVLGGERHALGGTFFQPTILKNVTQEMKVAREETFAPL
                     APIFKFETEEEVIQWANDTEFGLAAYLFSQNIGRIWRVSEQLEYGIVGINEGIISNEI
                     APFGGWKESGLGREGSKYGIEEFLEIKYLCMKV"
     gene            complement(8209..9519)
                     /locus_tag="PWY43_001593"
     CDS             complement(8209..9519)
                     /locus_tag="PWY43_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL2136461.1"
                     /translation="MIYAEIFVVLAAIYLGLRSGGLGIGLYGGLGLAILTLGFGLPMG
                     SIPVDVMLIIMTVVIGAAVLQAVGGMDLLVRYAEVLMRKNPKYINFIAPMITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRALAGSVVSSQLAIAGSPLSAAMAAMIAVMEN
                     NSVTFFQVIAVCLPVSFIASMVVAFVASKQGCELQNDVVYLERLKAGIVSPSRKLASE
                     STYSKGAKLLIGIFLLATVLVVLLAAVPGLKPVYENGKSMSTRDIIIVMMLASSCLMM
                     LVGKIKPDAIVASPIFRSGMTSIAVIIGIVTLGMTFVDAHLIEIKASIGNILTEYPML
                     LAVVLFFTCALLYSQGSTSALIIPLAVSLGIPNWAILASFIAITGIFILPTYPTSLAA
                     MELDTTGSTRAGKYIIDHPFMLPALVGVAVGLAFGFVWAPMIVS"
     gene            complement(9535..10230)
                     /locus_tag="PWY43_001594"
     CDS             complement(9535..10230)
                     /locus_tag="PWY43_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDL2136462.1"
                     /translation="MARNIIGILGGMGPAATADMFQKFIRLTPADCDQAHIPLLISSI
                     PDIPDRTRYILANGENPAPVMEKYIEGLESAGATCIIIACNTAHYWFEHLKAKSQVEM
                     LSMIDCVVDEVLKCGKRRVGLLATDATLAVELYKKQLEAQGLTFICPTENGQKAVMDS
                     IYLLKSGELEKATELMLQQRDELIRLGAEVIILGCTEVPIILAQEIEREPMRYVDSTL
                     VLVKKAIDWYQTH"
BASE COUNT         2991 a         2434 c         1995 g         2894 t
ORIGIN      
        1 gttttgcaaa atttttacaa aatcagaccg cttgtgttag ctatctataa tgctcctcgt
       61 ctgctcttcg cataggtgtc gaaggcaacg gcaaggatga tgattaagcc cataatgatt
      121 tgttggtagt aagcggaaac gtccattagt actaaaccgt taatcaatac acccataata
      181 atggagccga tgatggtgcc tgtgactcgt ccgtatcccc ccattaggga agttccgccg
      241 ataaccactg aggcaattac acgtagctca aaagaaacgc cggcaacgga ttcggcactg
      301 cctaaacggg cggagagaat gaaaccggca agtcctgcca atgctccgat caccacataa
      361 acactgacta aggtacggtt gatattcaca ccggctaaac gggctgcttc cggattgcca
      421 cctaccgagt acacatagcg tccccagcgg gttttatgca atgcgaagta accgataatg
      481 gcgactacgg caaaaatcac taccggcacc ggtacgccta aaatatcgcc acgtccccac
      541 cagcggtaac cttcgtcaaa tccggcaatt ggcgagccgt tatttaacac aagtgttaaa
      601 ccacgccaaa ttgtcatacc gccaagcgtg ataataaacg gtggcagttt gagtttagtg
      661 acgcctaagc cgtgtaacca accaatgaaa gtgcctaaac ctaaacagat actcaaacct
      721 atcagccagc tcattccgcc ccaagcaccg gcttctagcg ttaccgtatt atcgcctttg
      781 ataatatagg ccgcgaacat tgcggtaaca gcaaggattg agccgacaga gagatcgatc
      841 cctgcagtta agatgacgaa agtcattcct accgccataa tgccgaaaat cgacacttcg
      901 gttaaaacgt tatagatgtt gcgttgcgat aaaaatgccc cgttttgtga agtgaagaaa
      961 ataatcagta agatcaagaa aatcagtacg ccgaaacgtt caaagaaggc gataaaatcg
     1021 atttttcctt tgtcatcgac catacccata ttttttagaa attgcgttgt catacaaacc
     1081 tctcttatgc tgccgcttta cgctgaacgc caacagccat cattgacatt aatttttctt
     1141 cggtggcatc atcgccgtga atttcgccag ttacccgacc ttcggaaaga gtgataatgc
     1201 ggtcggaaat cgccataatt tccggtaaat cggaggaaat cactaatacc gcaacacctt
     1261 gttttgccat atcaaacagc acttggtgaa cctcagattt tgccccaaca tcaatacctc
     1321 gtgtcggctc atcaacaatt agtactttcg ggttgagtgc catacaacgg gctaaaatca
     1381 ctttttgctg attaccaccc gataatttgc gggcttcaag ttcgctactt gccattttga
     1441 tgcgtaacgc ttggcggtag gtttcgatta aatcatcttc tttttgatta tcgacaaaca
     1501 agttaccctt caataatttg cttaaaatcg gcagggtcat attggtttta atcggtaaac
     1561 cgagaactaa gccctctttt ttgcgatctt ccggcacaag ggcaacccct ttttccaaag
     1621 catctaacgg cgaggtggca gcataaggct taccgtctag gctgatttca tcagccgtat
     1681 aattttctac cccaaacaag caacgggcaa tttcagttcg ccctgcgcca actaagccgg
     1741 cgatccctaa tacttcaccc tcgtgaatgt taaaagagac accgtggaga gcaatgccgt
     1801 gtggatcgaa tgccggtttt tcccgatgca gatttttcac agctaaacgt attggtcggt
     1861 gggtatggtg agtctcttct ggtggacggc gagtaaaggc aacttcacgt cctaccatca
     1921 ttttaataat ttgatcgaca ttcacatttt tgacttcacc ctcaccagaa tagcgcccgt
     1981 cttggaaaac ggtgaaacgg tcgcataggg caaacacttc gtttaagcgg tgggtcacat
     2041 aaatcacgct aatgccacgg ctttttaaat cacgcacgat ttggtgtagt gtttccactt
     2101 ctttatcact taaggcagcg gacggttcgt ccataataat caacttagcg tttaaggtca
     2161 ttgcacgggc aatctcgacc atctgctgtt gtgccacact taaacgggaa acttgggtgt
     2221 caggtttgat atttaagccg agattatcca gcactaattg tgcttcctca ttcaccgctt
     2281 tttcattgac gaataaacct ttcaccggct ctctgcccaa aaagaaattt tcagcaaccg
     2341 tcatattcgg taacaagtta aattcttgat aaatcgttac gatgcctttg agttggcgat
     2401 caaacgggga atcgctcaaa cttaaggttt caccgttaaa gataatttcg ccacttgttt
     2461 gtggctgagc gcctgaaagt gcttttaata aggtcgattt tcctgcaccg ttttcaccca
     2521 acaatgcgtg aacttcgcct tttcccacag tcagtttcac gccattgagt gccataacac
     2581 cagagaaact ttttgtcaga tctttcactt caagtaaggg agtagacata caacctccgt
     2641 aaaaaaacgc cctccggcct cagccggaag gcagataaat tattcgctga tttctgcaaa
     2701 acgttctgct tcttgcagat tatctttcag gataattttt ggcggatagt ccgcaccgct
     2761 cacttctttg ctctcacgga tattgccaac cacttgctct aatgcggtgc ttaccgcaaa
     2821 acttgggcgt tggtcagcgg tgagatacaa ccagccatct ttcacacgtg caagagcctc
     2881 aggggttgag tcaaagccgg ttactaaaat atcgccggct ttcaaacctt ggctacgcaa
     2941 tgcttcaatc gcaccaagtg ccatatcgtc attcgctgaa ataatcactt ctggtttctc
     3001 ttttaaggtt ggtaacacac tttcaataat gcgtaatccc tcagaacgga gccagttacc
     3061 ggtttgatca accacgattt tatatttgtc accaccggca gctaattcat cacgaatacc
     3121 tttggtacgt tcaatgttag aggttgaacc cggctggccg gttaaaagaa taatttttgc
     3181 cccattcggg tatttggctt tcactgcttt tgcgacttct tgaccgcctg tgtagttatt
     3241 tgccccaaag tgtggaaccg gcttactgct ttcaactttt cggtctaatg tcgcagcagg
     3301 aattttttct ttgataattt cttccactgc acctgaaatc gcattcacat cattcgggga
     3361 aatgataatg cctttagcac cacgggtaat tgcattttct aagtcagaag cctgcttagt
     3421 agaagaactt tgcccgtcta atacttgcaa tttgatctcc aactttttac tggtttctac
     3481 cgctactttt tgtagttgaa cttcaaacgg gctggatagg ttcggtaagc taaataccac
     3541 aagctcatct ttcgcaaagc tgttcattgc taagcctaac gttaaagcac tcaatacaaa
     3601 tttaatacgc tttttcatta caaaatcctc tttttaagat gggaattgac ggcgaatatt
     3661 gccaatgtta cttcaaactg cgaaggaaac ttaagtctta tttcaataat aaacctcaat
     3721 gaaataaatt tttcaattta gaatatatca ttcaaaattt tccaaaccaa atgctttgtt
     3781 tcaaatttgt tacgtagatc acatttttag gaagagataa atgcaagcgg tcaaaacagg
     3841 tcgctttttt gcaaaaattt gaataaaaat gaccgcttgt gagatgaaaa ccaataaaaa
     3901 agcggtgatt tctcaccgct aaacactcaa aaaagaaatg tactcgttat tataaggttg
     3961 ggaaattaaa tgccgcttgg ctacgcaccg agctgtccgg ccaacgggtt gtgatggttt
     4021 tcatacgggt gtagaaacgc acgccgtctg tgccgtacac attgagaggc ccgaaaatag
     4081 aggctttcca accgccaaag cagtggaacg ccattggcac aggaattgga atattcacgc
     4141 ccaccatacc ggcttgtaca tcttgggtga attgacgtgc tgaatcgcca tctgcggtga
     4201 aaattgcact accattgccg tattggtgac tgttaatgag tgcgatccct tcggcataat
     4261 ctttcacacg cacaacagag agaaccggtc cgaagatttc atcttgccag atggtcattt
     4321 caggtgtcac gttatcaaac agcgtacctc cgacaaagta accgttttca aacccttcga
     4381 ctttatagcc tcgaccatcg acacagagtt tcgccccctg tgccacccct tgatcgatat
     4441 agctctcaat tttggcttta tgctctttgg aaatcaccgg ccccatatca ttttcaagca
     4501 cgccttgtgg tagtaagcct gaaccgattt tcaatgcttt gaccttagga attagctctt
     4561 caatgatttt atcggctaat tcatcaccaa caactaccgc taccgatagt gccatacaac
     4621 gttcaccggc tgcaccaaat gctgcgccca gtaaggcatt aattgtgcct tgtacatcag
     4681 catccggcat aatcaaagcg tggtttttcg caccgcccaa ggcttgaacc cgtttgccgt
     4741 ttgctgagcc tttctcataa atatattgtg caattgcagt tgagccgaca aaactcaccg
     4801 cctgaatgcg agagtcggtt aaaagaatat ctaccgcttc tttatcacct tgcaccacat
     4861 tgaatacacc gtccggcaag ccggcttctt ttaataattc tgccaagcga atagacacac
     4921 ttgggtcttt ttcagacggt tttaaaacga aagtgttacc gcaagcaagt gccactggga
     4981 acatccacat cggtaccatt gccgggaaat tgaacggggt aatgcctgcc actacgccca
     5041 acggttgctg gattgagtga atatcaatgc cacgccctgc attggcagag aactcacctt
     5101 tttgtaagtg tggaataccg gtggcaaatt ccaccacttc taaaccacgg gttaattcgc
     5161 caatcgcatc ggaatagatt ttgccgtgtt ctgagctgat taaacgagcc aactcatcaa
     5221 agtttgcttc caataactct ttgaatttaa ataaaattct ggcacggcgt agtggtgatt
     5281 gttgcgacca gcttttgaaa gcctgatcag ccactgcaat cgctgcatcg acttcgtttg
     5341 gcaggcttaa tttgactttt ttggtctctt caccggttgc gggattaaat accgaggtaa
     5401 cacgctggct ttggctttca acgactgcac cgttaataaa attttggaca aattggctca
     5461 ttggaggctc cttatcaact gattgagagg aaaaacggat aaaatacagc cgataaacta
     5521 ccgctaaaaa tggaaaatat caatacacaa tactaaaaaa tgaaaaattt atttcatttt
     5581 tgtgatctaa gtagcaagtt tacagattgc ctgtattaaa atgcgataaa atttcgtata
     5641 gtttgaagac gtattccatt tcgttaagcg aggtttccta tgtctagtcc attattgtct
     5701 aaacccaatt tcagccaacc tgagggatta attcaacaca ttacccctga aaatgcacag
     5761 tgggaatatg ttggatttga tgtttatcag ctcagtaagc aacaaattac gctggaaagt
     5821 ggtgaaaatg aactttgtct agtctttgtg accggttttg gttctgtcta tgttgatgat
     5881 tatacctttg aaaatatcgg caaccgtacc acaccatttg aaaaaattcc gccttatgcg
     5941 gtctatattc cgccacacaa aagcgtgttg attaaaactg aactcaattt agagttagca
     6001 gtgtgcaaag caccaagtca aggtcactat ccggtgcgtt tgattagccc gaaccatatc
     6061 ggtgtggaaa aacggggctt tggcaacaat caacgcttgg tgcataatat tctgccggaa
     6121 accgaaccag ccgacagttt gctggtggtg gaagtcttta ccgatgaagg caataccagt
     6181 tcctacccaa gccacaagca cgatgacaaa cactctaccg aagaaaccta tttggaagag
     6241 acctactacc accgcttcga tccaccacaa ggttttgcaa tgcaacgggt ttataccgat
     6301 gatcgcacac ttgatgaatg tatggcggtg tatgataaaa atgtggttaa agtgcctcgt
     6361 ggctaccacc ctgttgctac cattgcaggc tataataatt attacctaaa tgttatggca
     6421 gggccggtac gcaagtggaa atttacttgg gaacaagatc atctttgggt gaacagtgaa
     6481 acctataaag ccaaatttga gtaatacaag cggtcttttt ccaccaattt tttacaaaat
     6541 aagggattaa aaatgggacg cttttggcgt cccattcggt tatactttca tacaaagata
     6601 ttttatttct aaaaactctt caataccgta tttcgaacct tcccgcccaa gcccggactc
     6661 tttccagccg ccgaatgggg ctatttcgtt actgatgatc ccctcgttaa tcccgacaat
     6721 accgtattcc aactgttccg acactcgcca aattcgcccg atattttggc tgaataagta
     6781 agccgccaaa ccaaactcag tatcattcgc ccactggata acctcttctt cggtttcaaa
     6841 tttgaaaatc ggggcaaggg gagcaaaggt ttcctcacgg gcaactttca tttcttgagt
     6901 cacatttttt agaatagttg gttggaagaa tgtgccgccc agtgcgtggc gttcaccgcc
     6961 taacaccagt tctgccccaa gtgaaagggc atcgttaatg tggctttcga ctttttccac
     7021 cgctcgttca ctaattagtg cacccatcgt gacctcttcc tcaaatggcg agccaagctt
     7081 gatttgagcc actttctcac tcaattttgc aagaaattgc tcataaatgc ccgcttgcac
     7141 ataaattcgg ttagcacaga cgcaggtttg cccaccgtta cggaatttag aggcaaacac
     7201 gccctcaacc gctgtatcta gatccgcatc atcaaacaca atcgccggtg cattaccgcc
     7261 tagctccaat gccaattttt ttaccgtccc tgccgattgt tgcattaaaa ttttgccgac
     7321 acgggtggag ccggtaaagg tgagtttgcg tacggtcgga tgagtgctca actcccgccc
     7381 tataccttcg gcatcgctac tggtaataat gttaaatacc cctttgggaa tacctgcttg
     7441 atccgctaat tgggctaatg ctaaggcaga aagcggggtt tctgccgccg gtttaatcac
     7501 aatcgcacaa cctgccgcta acgccggtgc cgctttacgg gtaatcattg catttgggaa
     7561 attccacggt gtaattgctg ccaccacgcc aatgggttgt ttagtcacaa acaagcggcg
     7621 accggcttta tcttgtggca agacatcgcc ataaatccgc ttgctttctt cggcaaacca
     7681 ctcgataaaa ctggctccat aggcaatctc gcctcgactt tctgccaatg gtttaccctg
     7741 ttctagactt aaaatctggg cgagttcttc ttggttttgc ataatcagct caaaccattt
     7801 acgcaaaatc acggaacgct ctttgggcaa taatgcctgc cattttttct gggcaactgc
     7861 ggctgcttca atcgcttgag cggtttcttc cgtgcctaaa tcagccacct caccgatttt
     7921 ctcaccatta gtcggattct gtaccgtaaa ggttttccca ttttggcttg aaacaaattt
     7981 accgttaata taggcttgga aacggagtag caaaaatttg tgatttggca taatcactcc
     8041 ttgaatggtt aaaaagagaa caatgttgac tataatccaa tttcagtaaa gagaaaagag
     8101 ctgaaaaata actttttact caataataaa gcaccttaga caagcgttct tctaaggtgc
     8161 agccatttat tattgagaat taacttaaat tctctacata tttaataatt aagatacaat
     8221 cattggagcc catacgaatc caaaagcaag cccaacagca accccaacta aagctggaag
     8281 cataaacgga tgatctataa tatatttgcc agcacgagtt gaacccgtag tatctaattc
     8341 catcgctgct aatgaagttg ggtaggttgg caggataaaa atgcctgtaa ttgcaatgaa
     8401 agaagccaaa attgcccagt tagggatccc caagcttact gctaatggaa taataagagc
     8461 agaagttgac ccttgtgaat aaagtaatgc acaagtaaaa aagagtacta cagctaacaa
     8521 cattggatat tcagttaaaa tatttccaat agaggcttta atttctatta aatgtgcatc
     8581 gacaaaagtc atacctaatg taacaatccc gataattacc gcaatagaag tcattcctga
     8641 gcggaaaatt ggactagcca caatcgcatc aggtttaatt tttcctacca acatcattaa
     8701 gcacgaagag gctaacatca tcacaataat tatatctcgg gtactcattg acttcccatt
     8761 ttcatatact ggctttaacc ctggaacggc tgcaagtaga acaaccaata cagttgctag
     8821 taagaaaatg ccaatcaata gttttgctcc ttttgaatag gtagattcac tcgctaactt
     8881 acgagatggg ctgacgattc ctgcttttaa acgttctaaa taaacaacat cattttgcaa
     8941 ttcacagccc tgcttagaag caacaaatgc aacaaccata gaggcaataa aagaaacagg
     9001 taagcatact gctattactt gaaaaaatgt aacactgttg ttttccatta ctgctatcat
     9061 agccgccatt gcagcactca atggagaacc tgcaatagct aattgagaag acacaaccga
     9121 gccggctaat gctcttgaag ggcgaatacc actctctttt gccacttctg caatcacagg
     9181 taaggtagaa aagacgataa aacctgtacc agccataatc gtcattaacc aagtaatcat
     9241 cggtgcaata aagttaatat atttaggatt tttacgcatc aatacttccg cataacgaac
     9301 aagtaaatcc atacctccta ctgcttgtaa cacagcagca cctattacaa cagtcataat
     9361 aatcagcatt acatcaactg gaatagaacc cataggtaaa ccaaaaccta gagttagaat
     9421 cgcaagccct aaaccaccat atagtcctat tcctaagccc ccagaacgca gtcctaaata
     9481 gattgcagca agtaccacaa agatttctgc ataaatcata tccatctcct taaattaatg
     9541 agtttgatac caatcaattg cttttttaac caataccaac gttgaatcta cataacgcat
     9601 tggctcacgc tctatttctt gagctaaaat aataggtact tctgtacaac ctaaaataat
     9661 tacttctgca cctaaacgaa tgagttcatc acgttgttgt aacattagct ctgtggcttt
     9721 ctctaactca cctgatttta ataaatagat actatccatt actgcctttt gaccattttc
     9781 tgtcgggcaa ataaaagtca gtccttgtgc ttcaagctgt tttttgtata gttctaccgc
     9841 caaagttgca tcagttgcca acaatccaac ccgccgtttt ccacacttca aaacctcatc
     9901 aaccacacaa tcaatcatac ttagcatctc tacctgactt ttggccttca agtgttcaaa
     9961 ccaataatgt gcagtattac atgcaataat gatgcaggtt gcccctgcac tctcaagccc
    10021 ttcaatatat ttttccatta ccggagccgg attctctcca tttgctaaaa tatagcgagt
    10081 tctatctggt atgtcgggaa tagaggaaat caataatgga atatgagctt gatcacaatc
    10141 agctggtgtt aagcggataa atttttggaa catatctgct gttgctgcag gtcccattcc
    10201 acctaaaatg ccaataatat ttcttgccat acacttctcc ttataagatt taatttactg
    10261 cgatgactta tacaaacaag ttatgcaaaa aagaaatgca ataaatcgaa tcag
//
LOCUS       DANKBV010000076        10287 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.076, whole genome shotgun sequence.
ACCESSION   DANKBV010000076 DANKBV010000000
VERSION     DANKBV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10287
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.076"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(10..615)
                     /locus_tag="PWY43_001595"
     CDS             complement(10..615)
                     /locus_tag="PWY43_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HDL2136463.1"
                     /translation="MLDQTIIDQYDALIFDMDGTLIDTMPSHAKAWEKVGEVLGYPIN
                     PKPMYELSGSTTFVIAREIMQRSNIPEHYFEQVVQLKREFGIEMVLANATLLPAFEII
                     KANVGKKPMAIGTGSHRAMVELLDQKFNLRQYVSVIVDSDDVSNHKPAPETFLKCAEK
                     LGIAPHRCLVFEDADLGVQAALNGNMNVFDVRTNTITHSTR"
     gene            complement(636..1544)
                     /gene="rdgC"
                     /locus_tag="PWY43_001596"
     CDS             complement(636..1544)
                     /gene="rdgC"
                     /locus_tag="PWY43_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDL2136464.1"
                     /translation="MVWFKNVMIYKLTGELALESNSLNELQQETRFTPCSQHDMSKFG
                     WATPLTGSDLLHFSQANQFLLISHKEDKLLPANVVKAETEQRIAELEEKEARKLKKTE
                     KQAVKDQIVSALLPRAFSKHQFTALWLDLDNQLVYVDAASSKRAEDTLALLRKTLGSL
                     PVVPISFTLPPAEVMTNWIAKGHTPSWLNLLEEAELKSFDTDSIIRCKRQDLETEEIA
                     RHLQAGKFITKLAIEWEGHFNCVLNEDATLSRLKFADEVREKNDDILKEDVAQRFDAD
                     FLLMTEELKLFTEKMIAEFGGIKSRI"
     gene            1621..2439
                     /locus_tag="PWY43_001597"
     CDS             1621..2439
                     /locus_tag="PWY43_001597"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL2136465.1"
                     /translation="MNEKKLAFIGTGNMAYAIIQGLLKSGYSAEQIIACNKSNLARRE
                     ELQAVGIATNFANREAVEQADVIVLAVKPQMMAQVCAEFADVDFSNKWVISVAAGISV
                     QRLETLLPSAKNIIRTMPNTPSLISEGMTGLFAKKSVNPTACQFAEQLMSAVGKCYWV
                     ETEEQINHIIAITGSSPAYFFRFMEAMQQSAVQMGFSEQDARTLVQSVALGATKLVEA
                     NPTTPLSTLRENVTSKGGTTAKALEVFEQHDLAKTVDDAMWAAIHRAREMEQSL"
     gene            2436..3590
                     /locus_tag="PWY43_001598"
     CDS             2436..3590
                     /locus_tag="PWY43_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDL2136466.1"
                     /translation="MMKVSPFQWAAFNYFGFFCAYGVLLPFLPVWLKHHGYSTEMIGL
                     FAAVGYLFRFAGSMLASQRVKTVSQLIPTARSLTWLNIAAAVALLFSGDFIWLLFPVL
                     MLFQMFNSGAMPIADSIASIWQHQIGLDYGRARLFGSMAFVVGSLSAGYLIGLLGENS
                     VVWIIIGFLVFLGIGQLATPKVGFEEQGNKANTSSVTYRQLLKEPETLKMLIAVSLIS
                     ASHAAYYTYSTIHWSNASISTAMTSLFWGLAVVAEILFFLVSKRLFKSWKISHLMILA
                     AVFAVIRWAIMGSTTEIVLIIVMQMLHSITFGAAHIAMIRYISMQSAERVTKLQGLYF
                     GLASCAFMAVFTFIAGMVYQHSPSLTFWLMAIFTLPAIFIVPKRFEVQIN"
     gene            complement(3650..3862)
                     /locus_tag="PWY43_001599"
     CDS             complement(3650..3862)
                     /locus_tag="PWY43_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2136467.1"
                     /translation="MSAITGTVKWFNATKGFGFITPDQGGKDVFVHFSAIEANGGYRT
                     LEDGAKVQFEIQDSERGASAVNVKTI"
     gene            4190..4723
                     /gene="proQ"
                     /locus_tag="PWY43_001600"
     CDS             4190..4723
                     /gene="proQ"
                     /locus_tag="PWY43_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDL2136468.1"
                     /translation="MSEQIEQKVEQTQNKTNPSTKEVIAYLAEKFPLCFTLEGEVKPL
                     KVGLFQELAEALKDDEKISKTLLRQVLRNYTNSWRYLAASQANVARVGLQGEEVGVVT
                     EDEATHAAEALAAAKVVAERKAQERKAQRKEYFKQKAREENAKKRAEFKAKKAIDNLP
                     KASEEKLAALADKFGRK"
     gene            4809..6821
                     /gene="prc"
                     /locus_tag="PWY43_001601"
     CDS             4809..6821
                     /gene="prc"
                     /locus_tag="PWY43_001601"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDL2136469.1"
                     /translation="MKLTRLNKIFALALGLMIGNAFAVQPDIKESAIVMPKPNEQHSL
                     STKRVTARLTQSHYHKFQLNDEFAGKVFNRYVDWLDPSHNTFLQADIDEMRSKYATTL
                     DDDLYAGKLDAAFAMYELMSKRRYERYSYALSLLDKEPDLKGNDEIEIDREKAAFPKS
                     VEEANTLWEQRVKNDIINLYLKDKKWLEIKKTLTKRYNFAIKRLTQVKADDILQTYLN
                     SFSREIDPHTSYLSPRAAKAFQESMNLSLEGIGATLTQEDDVTSIRALVPGAPAAKSK
                     KLVVGDKIVGVGQGERGEIEDVIGWRLDNVVDKIKGKKGTKVRLEIEPEKGGKTKIIT
                     LVRDKVRIEDSAAKLTVEKVDGKNVGVIKIPTFYIGLTSDVRKLLTQIEKKNVEGLII
                     DLRENGGGSLTEVIELTGLFIKDGPVVQVQDAFNRIKVHEDPESKSVYDGKVMVMINR
                     HSASASEIFAAAMQDYNRAIIVGQQTFGKGTVQQSRSLNFVYDLDQEPLGFIQYTIQK
                     FYRINGGSTQLKGVEADIKFPEIINAAKTGESFEDNALPWDKIPAANYKEAGNARNAV
                     AALTAKHQERIAKNPEFIVLNENIAIRKERDERKFLSLNLEERLKENKADESKRLKDI
                     NARFAREGKKALKDIDDLPKDYEAPDFFLKEAEQMMLDWSEFDKKS"
     gene            6957..8444
                     /locus_tag="PWY43_001602"
     CDS             6957..8444
                     /locus_tag="PWY43_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HDL2136470.1"
                     /translation="MALAETATVAQVASEPVVQDNILPQLSFAAQQARAAELVKQADY
                     ALEQEQIRQEQARKFAEAEARIKQEIGNNALLLSQATTKVYLDSEFATMWNDKAAEKA
                     FLKEYALFAVSGVSSKSAKALQQILNQPEGLGRDILLTDGFLDYLYYNKNVFKNANNW
                     LYNLGSYVPKSPSSEQIFQWTNAVREGDSGQFVTNLVPRNHLYRDTAQRVLAMAGGSS
                     APKAKKGKAVTEVAGGGNGASFYKLALNAQRLRIIPSFNNGIFVNIPSYQLYYFRDGN
                     LALQSKVIVGREDRRTPVMDSKLSNVVVNPPWNVPTSIMTKDIIPKLARDPGYADRAS
                     FEILDSSGNKINPRSVNWSQYLNAKSSPYRIRQKAGDDSALGRYKFNMPSSDAIYLHD
                     TPNRGLFGKTGRALSSGCVRVEKSDELATILLKEAGWGSDKKNQVLGSKKTTSAQIRS
                     DNPVYLYYVTAWVEGGKVQTLPDIYNFDAKIPQHSVNWGKLKSII"
     gene            8614..9168
                     /locus_tag="PWY43_001603"
     CDS             8614..9168
                     /locus_tag="PWY43_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDL2136471.1"
                     /translation="MKQINVQRRKWLSLGGLVLGASLLPGQVMAALSTPAPAALRFRN
                     VNTGDTYTAKFGAGGLNKTDLSQLNYLMRDRHINQVKAIDPKLFVKLNQLQRRLGFHN
                     AEILVLSGYRSAQTNARLRRRSRGVASHSYHILGQAVDFQVSGVPLYKVKQAAESLNN
                     GGVGYYPRSNFVHVDTGPVRTWRG"
     gene            9185..9814
                     /locus_tag="PWY43_001604"
     CDS             9185..9814
                     /locus_tag="PWY43_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2136472.1"
                     /translation="MFNLQIIPVSPFQQNCTVIWDENKNAAIIDGGDNAEGIIQFVES
                     QQLKVQKLLITHGHLDHIMAVEKLREHFQVEVFGSHLADKPLFERLPEMCQQFGLPPV
                     NGFLPDHWLEEGDRVEVGELSYTVRHLPGHAPGHIGFFDFDNRIAFSGDVLFQNSIGR
                     TDLYLGNHSQLLETIREKMFDLDDDFIIIPGHGPHTTIGREKQTNPFLK"
     gene            complement(9849..10220)
                     /locus_tag="PWY43_001605"
     CDS             complement(9849..10220)
                     /locus_tag="PWY43_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDL2136473.1"
                     /translation="MRISYFAFAIFSIFLTACGSKPVSYLPTGSEPIVNIESDIAEKI
                     ELETNSKQFSTTNLTEFPLNLTYKLFWYNKDGVTQSHNGISDSIGWQHLWLQPKQKQT
                     IALIKPTEESHNYRLYLRGVK"
BASE COUNT         3066 a         1882 c         2281 g         3058 t
ORIGIN      
        1 ctatcatttt taacgggttg agtgagttat cgtattcgta cgtacatcaa acacgttcat
       61 attgccgttg agggcggctt gcacgcctaa atcggcatct tcaaacacca aacagcggtg
      121 tggggcaatg ccaagttttt ccgcacattt taaaaaggtt tcaggggctg gcttatggtt
      181 gcttacatca tcagaatcta caataaccga aacatattga cgtaagttaa atttttggtc
      241 taataattcc accattgcac ggtgtgagcc tgtgccgatt gccattggtt ttttgccaac
      301 gttagcttta ataatctcaa aggcaggcaa cagagtggca ttggctaaca ccatttcaat
      361 cccaaattcc cgttttagtt gtaccacttg ctcaaaataa tgctcgggga tattggagcg
      421 ttgcattatt tctcgggcaa tcacaaaagt ggttgagcca ctcagttcgt acattggctt
      481 tggattgatt gggtagccta acacttcgcc caccttttcc cacgccttgg cgtggcttgg
      541 catcgtgtca attaatgtgc cgtccatatc aaaaatcaag gcgtcatatt ggtcgataat
      601 ggtttgatct aacattgctt tccttaaatt tttttctaaa ttctggattt aattccgcca
      661 aattcggcaa tcattttttc ggtgaaaagt ttcagctctt cggtcattaa taagaaatcg
      721 gcatcaaagc gttgagccac atcttctttg agaatatcgt catttttctc tcgcacttca
      781 tcggcaaact tcaggcgtga aagcgtggcg tcttcattta acacgcaatt gaaatgccct
      841 tcccattcaa tcgcaagttt tgtgataaat ttgcccgctt gcaagtgccg ggcgatttct
      901 tccgtctcta aatcttggcg tttacagcga ataatgctgt cggtatcgaa agatttcagt
      961 tctgcctctt ccaacaaatt caaccaactt ggcgtatgcc ctttggcgat ccaattcgtc
     1021 attacctctg ccggcggtag ggtaaaggaa atcggcacaa ccggcaacga gccaagtgtt
     1081 tttcgcagta acgccagcgt atcttccgcc cgtttgctcg aggctgcatc cacataaacc
     1141 aactgattat ctaaatccaa ccaaagagcg gtgaattgat gtttgctgaa tgctcgaggc
     1201 aataacgcag aaacgatctg atctttcacg gcttgttttt cggttttttt cagcttacga
     1261 gcctcttttt cttccagttc tgcaatgcgt tgctcggttt cggctttcac cacattagca
     1321 ggtaataatt tatcttcttt gtgtgaaatc agcaaaaatt ggttcgcttg tgagaaatgc
     1381 agtaaatccg agccggtaag tggcgtagcc cagccgaatt tgctcatatc gtgctggctg
     1441 caaggggtaa agcgggtttc ttgctgtaac tcgttaaggc tattgctttc caacgccagc
     1501 tcgccggtta atttatagat catcacattt ttgaaccaaa ccatttttgc atatccttta
     1561 tcgggtgtag aatagtgtta ttttaacaga ccccaaccca acacagtaga gaatttgcga
     1621 atgaacgaga aaaaacttgc ctttatcggc accggaaata tggcgtatgc gattattcaa
     1681 ggcttattaa aaagcggtta ttctgccgaa caaatcattg cgtgcaataa aagtaatttg
     1741 gctcgccgag aggagttaca agcggtcgga attgcgacaa attttgcaaa tcgtgaggcg
     1801 gtagagcagg ctgacgtgat cgtgctggcg gtaaaaccac agatgatggc acaagtgtgt
     1861 gccgagtttg ccgatgttga tttttccaac aaatgggtga tttcagtagc agcaggtatt
     1921 tctgtgcaac gcttggaaac gctgttgccg agtgcgaaaa atatcattcg cacgatgcca
     1981 aacaccccgt ctttaatcag cgaaggaatg accggattat ttgcaaaaaa atcggtaaat
     2041 ccgaccgctt gccaatttgc cgaacagttg atgtcagccg ttggtaaatg ttactgggtg
     2101 gaaaccgagg agcaaatcaa ccacattatt gcgataaccg gctcaagccc ggcgtatttc
     2161 ttccgtttta tggaggcaat gcagcaaagt gcggtgcaaa tgggcttttc cgagcaggac
     2221 gcaagaacgc ttgtacaatc tgtcgcattg ggggcgacga aattggtgga agcgaacccg
     2281 actacgccac tttcgacctt gcgggaaaat gtgacctcaa agggaggcac aacggcgaaa
     2341 gcccttgagg tgtttgagca gcacgatctt gctaaaaccg ttgatgatgc aatgtgggcg
     2401 gcaattcatc gagccagaga aatggagcaa tcgttatgat gaaagtttcc ccgttccaat
     2461 gggcggcatt taattacttt ggcttttttt gtgcctatgg cgtgttgttg ccgtttttgc
     2521 cagtgtggct taaacatcac ggctattcga ctgaaatgat cggcttattt gccgcagttg
     2581 gctatttgtt ccgctttgcc ggcagtatgc ttgcctcaca acgggtaaaa accgtgagcc
     2641 aactcattcc caccgcccgc agtttaactt ggctgaatat tgcggctgcg gtggcgttgc
     2701 tgttttcagg ggatttcatt tggctacttt ttccggtgtt aatgttgttt caaatgttca
     2761 attcgggggc aatgccgata gccgatagca ttgcctcaat ttggcaacat cagatcgggc
     2821 tggattacgg cagagcaagg ttattcggct caatggcgtt tgtggtaggc tcactttctg
     2881 ccggctatct tatcggcttg cttggtgaaa attcggtggt gtggattatc atcggtttct
     2941 tggttttttt aggaataggg cagcttgcta cgcccaaagt cgggtttgaa gaacaaggta
     3001 ataaagccaa tacttcaagc gtaacttatc gccaattatt gaaagagccg gaaaccttaa
     3061 aaatgttgat cgcggtgtcg ttaatttcag cctcgcacgc cgcttattac acttacagca
     3121 ccattcattg gtcaaatgcc agtatttcaa ccgcaatgac tagcctgttt tggggcttgg
     3181 cggtggtggc ggaaattttg ttctttttgg tctctaaacg cttgtttaaa tcgtggaaaa
     3241 tcagccattt aatgatttta gctgccgttt ttgcagtgat tcgttgggca ataatgggta
     3301 gtactacaga gatcgtgctg attattgtaa tgcaaatgct acattctatt accttcgggg
     3361 cggctcatat tgcgatgatt cgctatattt cgatgcaatc ggcagagcgt gtgacgaaat
     3421 tacaaggtct ctattttggc ttggcaagct gtgcttttat ggcagtattt acctttattg
     3481 ccggaatggt ctatcaacat tccccaagcc tgacattttg gttgatggca atttttaccc
     3541 tacctgccat ttttattgtg ccgaaaaggt ttgaagtaca gattaattga tagcaaaaat
     3601 gccgacaagt tgtcggcatt tttatcgaaa ttcattgaat ttgctgaaat tagattgttt
     3661 tcacgtttac ggctgaagct ccacgctctg aatcttggat ttcaaattgt actttagcac
     3721 cgtcttctaa tgtgcggtag ccgccgtttg cttcgatagc agagaagtgt acgaatacat
     3781 ctttgccacc ttgatcaggg gtgataaaac caaaaccttt ggtggcgttg aaccatttta
     3841 ctgtgccggt aattgctgac ataaaatttt ccttatataa atacaaaaag tgataaaaga
     3901 tagctaaacg ggatattaca gacgtatcta tcaatcactt gccttgagga aaataactaa
     3961 caagattcaa ctttaattaa cgcacgaaca gcttgagcca ttacaccact tattcatttt
     4021 ttaagcaaat catttttata cttttatgaa cattcaaatg aaaaaagagt ctatctcttt
     4081 tgtaaacgta atgtggaatt tatatggacg aagccttata aatcaaatat agtatccagt
     4141 tagattttca cttatctaat tattagattt ttattaaatg ggacttatta tgtcagagca
     4201 aattgagcaa aaagtagaac aaactcagaa taaaaccaat ccaagtacca aggaagtgat
     4261 tgcttattta gcggagaaat ttccgctttg tttcacgctt gaaggtgaag taaaaccatt
     4321 gaaagttggg ttgtttcagg aattagctga agcattaaaa gatgatgaga aaatcagtaa
     4381 aacgttattg cgccaagtat tgcggaatta cactaattct tggcgttact tggctgccag
     4441 ccaagctaat gttgctcgtg tcggcttaca gggtgaagaa gttggcgtgg taacagaaga
     4501 cgaagctact cacgccgcag aagctttggc tgctgcgaaa gtggttgcag aacgtaaagc
     4561 acaagagcgt aaagcacaac gtaaagagta cttcaaacag aaagcacgtg aagaaaatgc
     4621 gaaaaagcgt gcagaattta aggcgaaaaa agcaatagat aatttaccta aagcttcaga
     4681 agaaaaatta gctgcattag cagataaatt cggtcgtaaa tagttttcaa gtcgctttgt
     4741 gcttacaagc ggtcggattt tagtaaaatt ttgcaaaaaa atgaccgctt gattatttgg
     4801 aaaagaagat gaaattaaca agattaaata aaatttttgc cctagcccta ggtttaatga
     4861 taggaaacgc ttttgccgtt cagccggaca ttaaagaaag tgcgattgtg atgccaaaac
     4921 ctaatgagca gcatagctta tcaaccaagc gtgtaacagc ccgattaact caatcgcatt
     4981 atcataaatt tcaactgaat gatgagtttg cagggaaggt ttttaaccgc tatgtcgatt
     5041 ggcttgatcc ttctcataat acttttcttc aagccgatat tgatgaaatg cgtagtaaat
     5101 atgctacaac attagatgat gatttatatg ctggtaaatt agatgcagca tttgcaatgt
     5161 atgagttaat gtctaaacgt cgttatgagc gttatagcta tgccttatct ttactagata
     5221 aagagcctga cttaaaaggg aatgatgaaa ttgagattga ccgtgagaag gccgcatttc
     5281 caaaaagtgt agaagaggca aatacacttt gggagcagcg agttaaaaac gatattatca
     5341 atttatatct taaagataaa aaatggcttg aaattaagaa aacactcact aaacgttata
     5401 actttgcgat taagcgttta actcaagtta aggctgatga tattttacaa acttacttaa
     5461 attcattctc tcgtgagatc gatccccaca ctagctattt gtctcctcgt gcggcaaaag
     5521 cgttccaaga gagcatgaat ctgtcgctag aaggaattgg tgcaacgcta acccaagaag
     5581 atgatgtaac cagcattaga gcattagtgc caggagcccc tgccgctaaa agtaagaagc
     5641 tggtagtcgg cgataaaatt gtcggtgtag gtcaaggtga gagaggtgag attgaagatg
     5701 ttatcggttg gcgtttggat aatgtagtcg ataaaatcaa aggtaaaaaa ggcactaaag
     5761 ttcgtttaga aattgagcct gaaaaaggtg gaaaaactaa aattattacc cttgtgcgtg
     5821 ataaagttcg tattgaggat agtgctgcta aattaacggt agaaaaagta gatggaaaaa
     5881 atgtaggggt aattaagatc cctacttttt atatcggttt gacttcagat gtacgtaaat
     5941 tacttactca aatagagaaa aaaaatgttg aaggattgat cattgattta cgtgaaaacg
     6001 gtggtggttc gttaactgaa gtgattgaat taactggttt atttattaaa gatgggcctg
     6061 tagtgcaagt acaagacgca ttcaatcgta tcaaagtaca cgaagatcca gaatcaaaat
     6121 cagtttatga tggaaaagtg atggtgatga ttaatcgcca tagtgcttca gcctctgaaa
     6181 tcttcgctgc ggcaatgcaa gattacaacc gtgcaattat tgtagggcaa caaacctttg
     6241 gtaagggaac tgttcaacag agccgttcat tgaattttgt atatgatttg gatcaagagc
     6301 ctcttggttt tatccaatat acgattcaaa aattctaccg tatcaatggt ggaagtaccc
     6361 agctaaaagg ggttgaggcg gatattaaat tcccggaaat cattaatgct gcaaaaacag
     6421 gtgaaagttt tgaagataat gcgcttcctt gggataaaat tccagcagct aattataaag
     6481 aggctgggaa tgctcgtaac gcggttgctg cattaacagc gaaacatcaa gagcgtattg
     6541 ctaaaaaccc agagtttata gtattaaacg agaatattgc gattcgaaaa gaacgtgatg
     6601 agcgtaagtt tttatcgtta aatttagaag aacgcctgaa agaaaataaa gcagatgaaa
     6661 gcaagcgctt aaaagatatt aatgctcgtt ttgcccgtga aggcaaaaaa gcattaaaag
     6721 atattgatga tttaccaaaa gactatgaag caccggactt tttcttaaaa gaagctgaac
     6781 agatgatgct tgattggtca gaatttgata aaaaatcata aatgtgttta taatgcgcac
     6841 ataacgtacc gcttagctct gctaagcggt ttattttttt ataaatttgc aaatattaag
     6901 aggaagagtg ggtgaaaacc tttaaattat tacctattat ggtggcgttt tctggcatgg
     6961 ctttggctga aacagccaca gttgctcagg tggcttctga gcctgttgta caagataata
     7021 ttttaccaca attaagtttt gcagctcagc aggctcgtgc tgcagaattg gtaaaacagg
     7081 ccgattatgc gttagaacag gaacaaattc gtcaagagca agcaagaaaa tttgcagaag
     7141 cggaagcaag aattaagcaa gaaataggta ataatgcctt attgctgagc caagcaacaa
     7201 ctaaggttta tttagacagt gaatttgcga caatgtggaa tgataaagcc gcagaaaaag
     7261 catttttgaa agaatatgca ttatttgcag taagtggggt atcctctaaa tcagctaagg
     7321 cattgcagca aatcttaaat caaccggaag gtttaggtag agatatttta ttaactgatg
     7381 gcttcttaga ttatttgtat tacaacaaaa atgtgtttaa aaatgcaaat aattggttat
     7441 ataacttagg cagttatgta ccaaaatcgc cctcttcaga gcaaatcttc caatggacta
     7501 atgccgtgag ggaaggcgat tctgggcagt ttgtaacaaa ccttgttcca agaaaccatt
     7561 tatatcgtga tacggcacag agagtattgg caatggcagg aggctcttca gcacctaaag
     7621 ccaaaaaagg taaagctgtt acggaggtag ctggtggcgg taatggggct tctttctata
     7681 aattagcgct caatgctcaa cgtttacgta ttattccaag ttttaacaat ggaatttttg
     7741 tgaatatccc aagctatcaa ttatattatt tccgtgatgg taatttagct cttcaatcta
     7801 aagtgattgt tgggcgagaa gatcgccgta caccggtaat ggatagtaag ctgagcaatg
     7861 tggtagtgaa tccaccttgg aatgtgccga ctagtattat gactaaagat attattccta
     7921 aactagcgcg tgacccaggt tatgcagatc gtgctagttt cgaaattctt gatagtagtg
     7981 gcaataaaat taatccgcgt agcgtaaatt ggtcacaata tttaaatgct aaaagttcac
     8041 cataccgtat tcgccagaaa gcaggtgatg atagcgcttt aggacgttat aaatttaata
     8101 tgccaagttc ggatgcaatt tatttgcatg atacgccaaa tagaggatta tttggtaaaa
     8161 cggggcgtgc gttaagttct ggttgtgtcc gtgtagaaaa atctgatgaa ttggcaacca
     8221 ttttattaaa agaagcgggt tggggatctg ataaaaagaa tcaagtctta ggcagtaaga
     8281 agacgacctc ggctcagatt cgttcagata acccggttta tctctattat gtgacggctt
     8341 gggttgaagg tggaaaagtg caaacccttc cggacattta taactttgat gccaaaatcc
     8401 cacaacattc tgtgaattgg ggaaaattaa agagcattat ttaatcgtgc tatttctgat
     8461 aaattccaag aagatatcat cttcttggaa ctttttgaaa atatagctta cataaattta
     8521 cgattgtaaa tttttacttt aagtatggtg tatagaaatt cgctcatttg tgagtgattt
     8581 tctataaatc attagctaac ctatgaggaa aaaatgaaac aaattaatgt tcaacgccgt
     8641 aagtggctat cactcggcgg acttgtttta ggggctagcc ttctgccggg tcaagtaatg
     8701 gcagcgcttt ctactcctgc cccagctgcg ttacgttttc gtaatgtgaa tacaggcgat
     8761 acttacactg ctaaatttgg tgcaggtgga ttaaataaaa ccgatttaag ccagttaaac
     8821 tatttaatgc gggatcgtca tatcaatcaa gttaaagcaa ttgatccaaa attatttgtc
     8881 aaattgaatc aattacaacg ccgtttaggt ttccataatg cggaaatcct agtattaagt
     8941 ggctatcgct ccgcacaaac aaatgcaaga cttcgtcgta gaagccgtgg tgttgccagc
     9001 catagttacc atattcttgg gcaagcagta gatttccaag tatcaggtgt gccgttatat
     9061 aaagtaaaac aagcggctga gagcttgaat aatggtggcg taggttatta tcctcgtagt
     9121 aactttgttc acgtagatac tggcccagtt cgcacttgga gaggctaaca agcggtctta
     9181 ttttatgttt aatttgcaaa ttattcctgt tagtccattt caacaaaatt gcacggtgat
     9241 ttgggacgaa aataagaatg cggctattat tgatggcggc gataatgccg aaggcattat
     9301 tcaatttgtt gaatctcagc agcttaaggt tcagaaatta ttgattacgc acggtcattt
     9361 agatcatatt atggcggtgg aaaagttgag agagcatttt caagtagagg ttttcggctc
     9421 gcatcttgca gataaacctt tatttgaacg tttgccggaa atgtgccaac aatttggatt
     9481 accaccagta aatggctttt tgccggatca ttggttggag gaaggtgatc gggtcgaagt
     9541 cggcgagctg agctataccg ttcgacattt gccgggtcat gctccgggtc atatcggttt
     9601 ttttgatttt gacaatcgta ttgcttttag tggcgatgtt ttattccaaa atagcatagg
     9661 tcgaacagat ctctatttag gcaaccatag ccagttattg gaaacgatca gggaaaaaat
     9721 gtttgattta gatgatgatt tcatcatcat accgggacat ggtccacaca caactatcgg
     9781 gcgggaaaaa cagaccaacc cgtttttgaa ataaataaag aagcagagca gttggctctg
     9841 cttttttgct attttacgcc tcgtaaatag aggcggtaat tatggctttc ttccgtcggt
     9901 ttaatgagtg caatggtctg tttttgctta ggttgcaacc atagatgttg ccagccgata
     9961 ctatctgata taccgttatg ggattgtgtt accccatctt tattatacca aaataattta
    10021 taagtaagat taagcggaaa ttcagttaaa tttgtggtgc taaattgctt gctattcgtt
    10081 tccagctcta tcttttcagc aatgtcagac tcaatattga caataggctc tgagccggtt
    10141 ggtaaatagc tgacaggttt gctaccacaa gcggtcaaaa aaatcgaaaa aattgcaaat
    10201 gcaaagtagg aaatacgcat agttattttg ccactaaagg tcctagtttt tgtccgccta
    10261 acaagtgcat atgaatatgg aacactt
//
LOCUS       DANKBV010000077        10230 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.077, whole genome shotgun sequence.
ACCESSION   DANKBV010000077 DANKBV010000000
VERSION     DANKBV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10230
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.077"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..446)
                     /locus_tag="PWY43_001606"
     CDS             complement(<1..446)
                     /locus_tag="PWY43_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2136474.1"
                     /translation="MKYKNLREFLDFLESQGELVRIQQEIDPYLEMTEIADRTLRKGG
                     PALLFENPKGYQMSVLCNLFGTPKRVALGMGQEDTKALRELGKLLAFLKEPEPPKGFK
                     DLIGQLPQWKQILNMPPKVLSKADCQQVVISGDEVDLYKLPIMHCWP"
     gene            583..1557
                     /gene="znuA"
                     /locus_tag="PWY43_001607"
     CDS             583..1557
                     /gene="znuA"
                     /locus_tag="PWY43_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDL2136475.1"
                     /translation="MFKRTLIGLAVLGASVAQADVLTSIKPLGFIASAITEGVTETKV
                     LLPVTASPHDYSLKPSDVTQLQSAQLVVWVGDGLEGFLEKSIEKLPKEKVLTLEDVDA
                     IDDIVDKASKKEDKDDDHDHKHEHKHEHKHEHKHEGHNHEGHNHDHHGHSHDEDWHIW
                     LSSEASELAAEQIAARLSEQMPEHKAKIAENLAKFKANLTAKKAEIAKQLEPVKAKGY
                     YTFHDAYGYFEREYGLNSLGSFTINPTVAPGAKTLTAIKKNIAKNKAQCLFAEPQFTP
                     KVIETLAKETKVNVGQLDPLGAKVQLSATAYPEFLQVLADEFSQCLTK"
     gene            complement(1609..1929)
                     /locus_tag="PWY43_001608"
     CDS             complement(1609..1929)
                     /locus_tag="PWY43_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HDL2136476.1"
                     /translation="MIYPTCPECKSENTYHDSIQFVCPDCAYEWTGEETDQDDDVLIV
                     KDSNGNLLSDGDDVLLIKDLKLKGSSEVLKKGTKFKGIRLAQGDHNVDCGKIMLKSEF
                     LKKA"
     gene            2070..4433
                     /gene="rnr"
                     /locus_tag="PWY43_001609"
     CDS             2070..4433
                     /gene="rnr"
                     /locus_tag="PWY43_001609"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL2136477.1"
                     /translation="MIVDPNYQQELEKYENPVPSREFILNTIREHNAPMSRDELLSAF
                     HIDDEERMEGIRRRLRAMENDGELVFTKGKRYALPEKMDLIKGTVIGHRDGYGFLQVE
                     GHEKGATKSEDWFIPNAQMVKVMHGDFVLAQPNGTDRRGRKEVRIVRVLEARKKQIVG
                     RFFLESGIGFVVPDDSRVNQDILIPDEHRMGARMGQVVVVELQERKASFSRPVGIITE
                     ILGDNLAPGMEIEIALRNHDIPHIWPEGVEKQIRQFSSEEVPEEAKQGRVDLRDLPLI
                     TIDGEDARDFDDAVFAKKEGNGWRLWVAIADVSYYVRPKTALDLEALNRGNSVYFPNR
                     VVPMLPEVLSNGLCSLNPQVDRLCLVAEMSVSSKGELQDYQFYEAVMNSHARLTYTKV
                     WKMLEGDEALRERYAPLVPHIEELYAMFQTLMKARHKRGAIEFETIENQFIFNPQGRI
                     ERIEPLIRNDAHKLIEECMILANIAAARFVEKANEPALYRIHDKPSEEKLLSFKSFVR
                     ECGLTWDVGLDPTPKDYGVLLEQIAERADKELIQTMLLRSLKQAVYAADNIGHFGLAL
                     TEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRYYTDGGGYHYKLDDMDEFGDKCSA
                     TERRADEATREVADWLKCEFMQDHLGEEFDGVISSVTGFGLFVKLNELLIDGLVHIST
                     LDNDYYHYDADRQRLIGGSGIIYRLGDAVRVKVINVNLDEKKIDFELLTGNKKTGKTA
                     KKKAKATEVPVFKEPKTVKKAKKVAEKKPAKSAKKRTKTTASKSRKK"
     gene            4437..4703
                     /locus_tag="PWY43_001610"
     CDS             4437..4703
                     /locus_tag="PWY43_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136478.1"
                     /translation="MDYKKQILFSLLFALVIAIISYFLFTYIPFDCSDLSKLNDSQRL
                     ECRTAEVLGVYYWLPHTTTLFFGSVLLYWAVYGMISLVQAVLKR"
     gene            4927..5385
                     /gene="mraZ"
                     /locus_tag="PWY43_001611"
     CDS             4927..5385
                     /gene="mraZ"
                     /locus_tag="PWY43_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL2136479.1"
                     /translation="MFRGASSISIDSKGRIAIPTRYRAELLEKHHGILVCTVDIRQPC
                     LLLYPLHEWEMVEQKLLALSNFDPVQRRIQRVMQGFATECEMDSAGRILLSPTLRQHA
                     QLEQQIMLVGQLNKFEIWQDKQWQAQIAEDLAFGSSAEILDCEALKNLSL"
     gene            5401..6339
                     /gene="rsmH"
                     /locus_tag="PWY43_001612"
     CDS             5401..6339
                     /gene="rsmH"
                     /locus_tag="PWY43_001612"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL2136480.1"
                     /translation="MSENPKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSK
                     LGENGRLIATDRDPRAIAEAQTISDPRFQIEHTAFSAIPELCERMGLVGKIDGILLDL
                     GVSSPQLDEAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSAEDLAWVLKEFGEERF
                     AKRIAQAVVSYNKSANEKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINSEL
                     DELEKALNSALTVLAPEGRLSIISFHSLEDRMVKQFVRKHSKGMDVPRGLPILEAELT
                     KNIPLKTIGKAIMPSEAEIEANARSRSAVLRIAEKR"
     gene            6358..6669
                     /gene="ftsL"
                     /locus_tag="PWY43_001613"
     CDS             6358..6669
                     /gene="ftsL"
                     /locus_tag="PWY43_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2136481.1"
                     /translation="MSNERYPLHQIIIDDIFSHNKVALLLLIGVVISAVATIWITHQT
                     RLSVSEQGQLIQANQKLESQYTNLQLEENSRSSRARVDAAAKSFGLQPIKKEQEIILV
                     E"
     gene            6673..8733
                     /locus_tag="PWY43_001614"
     CDS             6673..8733
                     /locus_tag="PWY43_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL2136482.1"
                     /translation="MVKSVKLKRKQEAPVAKAEPQQAEGKKTPSFLPTRFRVVVFFML
                     LMVGGLLAQTAYIQLFDSERLIKEANNRSLRTKELQFTRGRILDRSGRFLSISVPMYS
                     LTLDPREYFDTKLRRSNESWRALAIEMETSKGKIEANVNKFIEKKNFSKEKVDFDPRS
                     ILNTKSEGYWTQLAQVTGLDYNSLIEKVRNNPSSVFLRLDNENLVRERQKFQALSKEI
                     GRSYNDIMDELYSKNRQRFLYLSRHQSEAISEYAKELDIDALVIKVESRRFYPLAEES
                     SQLIGFTDKDDMHGLEGLERSFDSLLIGKNGKQIIRKDAKGNIVENIRSEKQYDPQDV
                     MLSIDEELQSMVYGEIKKAVQENNAESGTAVLVDVQTGEILAMANAPSFNPNKRDSFK
                     PELMRNRAITDTFEPGSTVKPFTVLTALQNGVTYRDEVIGTRPFVVNGHTIKDVAPRD
                     SLTLTGILQKSSNIGVSKLALRMPANALVDTYTKVGFGKDTGLGLGEQRGTNGDRKRW
                     ADIERATLSYGYGLNVTPLQLARAYATLGSFGLYRPLSITKVDPPVIGERVLPEKTTR
                     DVVHMMESVAAKGEGGHRAAVDGYRVAIKTGTARKLEKGKYVEKYIAYTAGLAPASDP
                     RFALVVLINEPKAGNYYGGFVSAPLFSRIMGYTLKQRNIKPDNLTENTQSAVREIKLE
                     RPIN"
     gene            8742..>10230
                     /gene="murE"
                     /locus_tag="PWY43_001615"
     CDS             8742..>10230
                     /gene="murE"
                     /locus_tag="PWY43_001615"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDL2136483.1"
                     /translation="MKRLLPFLTELDAWVEELTELKQMTLDSRQVQAGDLFVALKGHQ
                     VDGRKFIPNAIEQGAAIILAEADEDQPEAELDSKFAKFNLDRTACCKVVSVPNLPKLL
                     SEIAGAFYHNPSHKLTLAGITGTNGKTTTAQLLAQWHNLLGGKSAVMGTIGNGLYGQV
                     QEAVNTTGSAIEIQRNLANFVELGADFCAMEVSSHGLAQFRAEALDFDLAIFTNLSRD
                     HLDYHNTMEEYAQAKFRLFNELSTKAQVINADDEIGREWLTQLPNAVAVSTDPEFDSN
                     HRFVKATAVKFTLQGASIAFKSSWGNGELHSRLIGAFNVNNLLTAFAGLLALGFDIQD
                     LIKTSPNLVGVAGRMECITAPNKPMVIVDYAHTPDALEKALQAARLHCEGELWCIFGC
                     GGDRDAGKRPLMATIAEKLTDKVIATDDNPRTEDNQKIMADIVKGFSKPQQIIHNREE
                     AIKTAIEQAKATDVILIAGKGHENYQIIGTEKLHFSDQETARKYLF"
BASE COUNT         3182 a         2025 c         2292 g         2731 t
ORIGIN      
        1 ggccaacagt gcataatcgg taatttgtag agatcaacct catcacccga aatcaccact
       61 tgctgacaat cggctttgct cagtacctta ggcggcatat ttaaaatttg cttccattgt
      121 ggcagttgcc cgatcaaatc tttaaaccct ttcggtggtt caggctcttt taaaaaggca
      181 agtaacttgc ccagctcacg cagagctttg gtatcttctt gccccattcc cagagctacc
      241 cgtttgggcg tgccgaataa attacacaac accgacattt gatagccttt cgggttttca
      301 aacagtaatg ccggcccgcc tttgcgtaag gtgcggtcgg cgatttctgt catttctaaa
      361 taaggatcga tttcttgctg aatacggact aattcaccct gagactcaag gaaatcgaga
      421 aattcacgga gatttttata cttcatagcg gtcatatttt tcgatttttt tgcaagtata
      481 tagaaaaacg taaatgaaaa atacccataa aaaatttata gaaattttgt tttattttgt
      541 tagaatagct cggttcaaat taaatgccat aaggggaatt ttatgtttaa aagaacatta
      601 atcggtctag ccgtgttagg tgcaagtgtt gctcaagcgg atgtattaac cagtattaag
      661 cctttaggct ttattgccag tgccattacc gaaggggtaa ctgaaactaa agtattatta
      721 ccggtaactg cctctccaca cgattacagc ttaaaacctt cagatgtgac tcaattacaa
      781 tctgctcaat tagtggtttg ggtgggtgac ggcttggaag gctttttaga aaaaagtatt
      841 gaaaaactgc cgaaagaaaa agtattaacc cttgaagatg ttgatgcgat tgatgatatt
      901 gtcgataaag caagcaaaaa agaagataaa gacgatgatc acgatcataa acatgaacat
      961 aagcacgaac acaagcacga gcataaacac gaagggcata atcacgaagg acataaccac
     1021 gatcatcacg gacatagcca cgatgaagat tggcatattt ggctctcaag cgaggcaagt
     1081 gagctggcag cagagcaaat tgcggcacgt ttaagcgaac aaatgcctga acacaaagcg
     1141 aaaattgcgg aaaatcttgc aaaatttaaa gcaaatttaa ccgctaaaaa agccgaaatt
     1201 gcgaaacaat tagagccggt aaaagccaaa ggttactaca cattccacga tgcttacggc
     1261 tattttgaaa gagaatatgg tttaaattca ttgggttcat ttaccattaa cccaactgtt
     1321 gccccgggtg caaaaacact tacagcaatc aagaaaaaca ttgcgaaaaa caaagcacaa
     1381 tgtttatttg ccgagccgca atttacgccg aaagtaattg aaaccttagc aaaagaaact
     1441 aaagtaaatg ttggccaatt agacccactc ggtgcaaaag ttcagttaag tgcgactgct
     1501 taccctgaat ttttacaagt tttggctgac gaatttagcc aatgtttaac aaaataagtt
     1561 aaaaatcccc catttaaata taaatggggg attttgcgat tacatcactt aagctttttt
     1621 taagaattct gatttcaaca taattttacc gcaatcgaca ttatggtcac cttgagctaa
     1681 acggattcct ttgaattttg tacctttttt cagtacttca gacgagcctt ttaattttaa
     1741 atctttgatt aataatacat cgtccccgtc agataataaa ttgccattac tgtcttttac
     1801 aattagcaca tcatcatctt gatccgtttc ttcgcccgtc cattcatacg cacaatccgg
     1861 gcaaacaaat tgaattgagt cgtggtaagt attttcgctt ttacattcag ggcaggttgg
     1921 gtaaatcatt ttaattcctc aaattaataa agaaccagat tatataccga aatccagcaa
     1981 aacgctaagc ggtcatattt tgccgatttt ttaccaatcg agctaaaagt cagttagaat
     2041 agccttaatt acacaaaaag gaggatacta tgatagtcga tccgaattat cagcaagaat
     2101 tagaaaaata cgaaaatccg gtaccaagcc gagagtttat tttaaataca attcgtgaac
     2161 ataacgcccc aatgagccgt gatgaattat tgtcggcttt tcatattgac gatgaagaac
     2221 gaatggaagg cattcgccgc cgtctgcgtg caatggaaaa tgacggtgaa ttggtgttca
     2281 ccaaaggcaa acgctatgcg ttgccggaaa agatggattt aatcaaaggt acagttatcg
     2341 gccatcgtga cggttacggt tttttgcaag tggaaggaca cgaaaaaggg gcgactaaaa
     2401 gcgaagattg gtttattccc aacgcccaaa tggtaaaggt aatgcacggt gactttgtgc
     2461 tggcacaacc aaacggcaca gaccgcagag gtcggaaaga agtccgcatt gtgcgagtgt
     2521 tggaggcccg taagaaacag attgtcgggc gttttttcct tgaaagcggt atcggttttg
     2581 ttgtgcctga tgacagccgc gttaaccaag atattctcat tccggatgaa caccgtatgg
     2641 gggcgagaat ggggcaagtt gtggtggtgg aattacagga aagaaaagcg agttttagcc
     2701 gcccggtggg aatcattact gaaattctag gcgataattt agcgccggga atggaaattg
     2761 aaattgccct acgcaatcac gatattccgc acatttggcc ggaaggggta gaaaaacaaa
     2821 tccgccaatt tagtagcgaa gaagtgccgg aagaagccaa gcaagggcgg gtggatttgc
     2881 gagatctgcc actgattacc attgacggcg aagatgcccg agatttcgat gatgctgtat
     2941 ttgccaaaaa agagggcaac ggctggcggt tatgggtggc gattgccgat gtaagttatt
     3001 atgttcgccc aaaaaccgca ttagatttgg aagccctaaa tcgtggtaac tcggtctatt
     3061 tcccgaatcg ggttgtgcca atgttgccgg aagtgctttc caatggcttg tgttcactca
     3121 atccacaggt tgacaggctc tgcttggtgg cggaaatgtc ggtttccagc aaaggggaat
     3181 tacaagatta tcaattctac gaagcggtaa tgaactctca cgcccgttta acttacacta
     3241 aagtgtggaa aatgttggaa ggtgatgaag ccttgcgtga acgctatgct ccattagtgc
     3301 cgcatattga agaactctat gcaatgttcc aaaccttaat gaaagctcgc cataaacgag
     3361 gggcgattga atttgaaacc attgaaaatc aatttatttt caatccgcaa gggcgaattg
     3421 agcgaattga gccgttaatc cgcaacgatg ctcataaatt gattgaagaa tgtatgattt
     3481 tagccaatat tgctgccgct cgttttgtgg aaaaagcgaa cgaaccggcg ttatatcgga
     3541 ttcacgataa gccgagtgaa gaaaaattgc tcagttttaa atcctttgtg cgtgagtgtg
     3601 gcttaacctg ggacgtaggc ttagatccaa ccccaaaaga ttacggtgta ctactggagc
     3661 aaattgccga acgtgccgat aaagagttga ttcaaacgat gttgctccgc tcgctgaaac
     3721 aagcagtgta tgcggcggat aatatcgggc atttcggctt ggcattaacc gaatatgctc
     3781 attttacctc gccgattcgc cgttacccgg atttactgtt acaccgtgca attaaatatc
     3841 tgatcgaaaa aggcaaaggc aacactcgct actataccga tggcggtggt tatcactaca
     3901 agttagatga tatggacgaa ttcggcgata aatgttctgc caccgaacgc cgagctgatg
     3961 aggcaacccg agaagtggca gattggctga aatgcgaatt tatgcaagat catcttggcg
     4021 aagagtttga tggcgtgatt tcgagtgtta ccggctttgg cttatttgta aaactcaatg
     4081 agcttttaat tgacggattg gttcatattt caacgctgga taatgattat taccactatg
     4141 atgcggaccg acaacgttta atcggtggca gtgggatcat ctatcgttta ggcgatgcag
     4201 ttagagtaaa agtaatcaat gtgaatttag acgagaaaaa aattgatttt gagttactca
     4261 ccggtaataa gaaaaccggc aaaaccgcca agaaaaaagc aaaagcgaca gaggttcctg
     4321 ttttcaaaga gcctaaaacg gtaaaaaaag ccaaaaaagt cgctgagaaa aaaccggcaa
     4381 aatctgcaaa aaaacggaca aaaacgaccg ctagtaaatc aaggaaaaaa tagcaaatgg
     4441 attacaaaaa acaaatttta ttcagccttt tatttgcttt agtaattgca ataataagct
     4501 attttttgtt tacctatatc ccgtttgatt gtagcgatct gagcaaactc aacgattcac
     4561 aacgtttaga gtgccgaact gccgaagttc tgggcgttta ttactggctg ccgcacacta
     4621 ccacgctgtt tttcggctcg gtgttattat attgggcggt ttatggcatg atttcattag
     4681 tgcaagcagt gctcaaacgc taacaagcgg ttttgttttt gcaaaaagtt gcaaatctca
     4741 tctgaataat ccttccaaga tcaaaggtct atcaaaaaag atagaccttt attgtgtaaa
     4801 taaactaaaa ctccttgata gatgtatcac atatctataa aattccagca attaagtggg
     4861 gaaaaagtgg taaattgtgg aacttttcct taaatttaac ctcatttaaa aaggtaaatc
     4921 agtaaaatgt ttcgtggtgc aagttcaatc agcattgata gcaaagggcg gatcgctatt
     4981 ccgacccgtt atcgtgccga gctattggaa aaacatcacg ggatcttagt ttgcactgtg
     5041 gatatccgcc agccttgttt gttgctttac ccacttcacg aatgggaaat ggttgaacag
     5101 aaattgttgg cactatctaa ttttgaccct gttcaacgcc gtattcagcg agtaatgcaa
     5161 ggctttgcta ccgaatgtga aatggattcg gcagggcgaa tactgcttag ccctacactt
     5221 cgccaacacg cacaattaga gcaacaaatt atgctggttg gacaattaaa caaatttgaa
     5281 atttggcaag ataaacaatg gcaagcacaa attgccgaag atcttgcttt tggcagttcg
     5341 gcagaaattt tagactgcga agcattgaaa aacctgtctc tctagtaatt ggaaattgag
     5401 atgagcgaga acccaaaaca tattacagta ttattgcacg aagcggttga cggcttggca
     5461 attaagccta acggtatcta tattgacggt actttcgggc gtggcgggca ttctcgcttg
     5521 attttatcca aactgggcga aaatgggcga ttaatcgcaa ctgatcgtga cccacgagcg
     5581 atagccgaag ctcaaacgat tagtgaccct cgttttcaaa ttgaacatac ggcattttct
     5641 gccattcccg aactgtgtga acgaatgggg ttggtgggca aaattgacgg tattttattg
     5701 gatttaggtg tgtcttcccc gcagcttgac gaagccgaac gaggctttag ttttatgcgt
     5761 gacgggccgc ttgatatgcg aatggatacc accaaaggct tatcggcagc cgagtggctt
     5821 gcccaagttt cggcagaaga tttagcttgg gtattgaaag aatttggcga agagcgtttt
     5881 gccaaacgga ttgcacaagc ggtcgtttct tacaataaat ctgcaaatga aaaaatcagc
     5941 cgcactttgc agcttgcgca aattattgcg gatgcggtgc cgtttaaaga taagcataaa
     6001 caccctgcaa cccgttcgtt ccaagcaatt cgcattttta tcaatagtga attagacgag
     6061 ctggaaaaag ctttaaattc cgcattaacg gtgttagccc ctgaagggcg tttgtcgatt
     6121 atcagctttc attctctaga agaccgaatg gtgaaacagt ttgtgcgtaa gcatagtaaa
     6181 ggaatggacg taccccgtgg tttaccgatt ttagaagcag agctaaccaa aaatattccg
     6241 ctgaaaacca ttggcaaagc gattatgccg agcgaagccg aaattgaagc aaatgcccgc
     6301 tcacgcagtg cggtattgcg gattgccgaa aaacgctaac agtaaatagg gaagaatatg
     6361 tcgaacgaac gttatccgtt acatcaaatc atcattgatg atatctttag ccataacaaa
     6421 gtggctcttt tattgttgat tggtgtagtg atttcagcag tggctacgat ttggataacc
     6481 caccagactc gtttatcggt gagcgaacaa gggcaactga ttcaagcaaa ccaaaagttg
     6541 gaaagccaat atactaatct gcaattagaa gaaaacagca gaagcagtcg ggcaagagtt
     6601 gatgccgccg ctaaatcgtt cggattacag ccgattaaaa aagagcaaga aattatttta
     6661 gttgaataaa aaatggttaa atcagttaaa ttaaaacgta agcaagaagc tcctgtcgca
     6721 aaagcagagc ctcaacaagc agaaggtaaa aaaacaccga gttttttacc tactcgcttt
     6781 agagtggttg tgttttttat gctattaatg gttggtggct tactagcaca aaccgcctat
     6841 attcagcttt ttgactctga aagattaatt aaagaggcta ataaccgctc acttcgtact
     6901 aaagaactcc aatttacccg tggacgtatt ttagatcgta gcgggcgttt tctctctatt
     6961 agtgtgccaa tgtactcatt aacacttgac ccgagagaat actttgatac taagctgcgt
     7021 cgttctaacg aaagctggcg tgccttagcg attgaaatgg aaacatcaaa aggtaaaatt
     7081 gaggcaaacg ttaataaatt tattgaaaag aaaaattttt caaaagaaaa agtagatttt
     7141 gatccacgtt ctattttaaa tactaaaagt gaaggttatt ggactcagct tgcccaagtt
     7201 acaggtttgg attacaacag tttaatagaa aaagtacgga ataaccctag ctcggttttt
     7261 ttacgtttag ataacgaaaa tttagtaaga gaacgccaga aattccaagc gttaagcaaa
     7321 gaaatcggcc gttcctataa tgacataatg gacgagcttt atagcaaaaa ccgccagcga
     7381 ttcctttact tatcccgtca tcaatctgaa gcgatcagcg aatatgcaaa agaactagat
     7441 attgatgcat tagtgattaa agtcgaatca cgccgttttt acccgctggc agaagaatct
     7501 tcacagttga tcggttttac tgataaagat gatatgcacg gtttggaagg gctggagcgt
     7561 agttttgatt ccttacttat cggcaaaaac ggtaaacaaa ttatccgaaa agatgccaaa
     7621 ggcaatattg tggaaaatat ccgttcggag aaacagtacg atccgcaaga tgttatgtta
     7681 agcattgatg aagaattgca gtctatggtg tacggtgaaa tcaaaaaagc ggtacaggaa
     7741 aataatgcgg aatcaggcac tgcggttttg gttgatgtgc aaaccggcga aattttggca
     7801 atggcaaatg ccccttcatt caacccaaac aaacgggata gttttaaacc tgaattaatg
     7861 cgtaaccgtg ctattaccga cacttttgaa ccgggttcaa cggtgaaacc ttttaccgta
     7921 ttaaccgcat tacaaaatgg ggtaacttat cgtgatgaag ttattggcac tcgcccattt
     7981 gtcgtcaatg gacacacaat taaagacgtt gctccaaggg acagccttac gcttaccggc
     8041 atcttgcaaa aatccagtaa catcggggtc agtaaattag ctctcagaat gccggcaaat
     8101 gcgttagtgg atacttacac gaaagtcggt ttcggtaaag acaccggttt aggcttgggt
     8161 gaacaacgag gtacgaacgg tgatcgcaaa cgctgggcgg atattgaacg tgctaccctt
     8221 tcctatggat acggtttaaa tgtgacccca ttacaacttg cccgtgctta tgcgacttta
     8281 ggaagtttcg ggctgtatcg cccactttcg attaccaagg tcgatccgcc ggtgattggc
     8341 gaacgggtgc taccggaaaa aaccacccga gatgtcgtgc atatgatgga aagcgttgcg
     8401 gctaaaggcg aaggcggaca ccgtgcagcg gtggacggct accgtgtagc catcaaaacc
     8461 ggtacagcac gcaaattaga aaaaggcaaa tatgttgaaa aatatattgc ttataccgca
     8521 gggcttgccc cggcaagcga tcctcgcttt gcattagtgg tattaatcaa cgaacctaaa
     8581 gcaggcaact attatggtgg ctttgtttct gcaccactgt tctcacgcat tatgggttac
     8641 accttaaaac aacgtaatat taagccggat aacttaacgg aaaataccca aagtgccgtg
     8701 cgtgaaatta aattagaacg ccctatcaat taaggttagc tatgaaacgt ttactccctt
     8761 ttctgacaga acttgatgct tgggtggaag aactcaccga gctaaaacaa atgacacttg
     8821 atagtcgcca agttcaagcc ggtgatctct ttgttgcttt aaaaggacat caagtcgatg
     8881 gcagaaaatt tattccgaat gcgatagaac aaggagcagc aattattttg gctgaggctg
     8941 atgaagatca gccggaagct gagcttgatt caaaatttgc aaaatttaat ctagatcgta
     9001 ccgcttgttg caaggttgtt tcagtaccga atttgccaaa attgctatct gaaattgcag
     9061 gggcttttta ccataacccg tcacacaagc tgactttagc aggcattacc ggtacgaacg
     9121 gtaaaacgac taccgctcaa ttattggcac aatggcataa tttattgggt ggaaaatcgg
     9181 cagttatggg tactatcggc aacggtttgt acggacaagt gcaagaagcg gtaaatacca
     9241 ccggctccgc cattgaaatt cagcgtaatc ttgccaactt tgttgaactt ggcgcggatt
     9301 tttgtgcgat ggaagtcagc tctcacggtt tggctcaatt tagagcggaa gccttagatt
     9361 ttgatttggc gatctttacg aatttaagcc gagatcatct cgattaccac aacacgatgg
     9421 aagagtatgc ccaagccaaa ttccgtctct tcaacgaatt aagcactaaa gctcaggtga
     9481 ttaacgccga tgatgaaatc gggcgtgaat ggctgacaca attaccgaat gcggttgcgg
     9541 taagtactga tccggagttt gatagcaatc atcgctttgt caaagccacg gcagtgaaat
     9601 ttaccctgca aggggcaagc attgcgttta aatcaagctg gggaaatgga gaattacata
     9661 gccgtttaat cggggctttc aatgtaaata atctgcttac cgcctttgcc ggacttttag
     9721 ctcttggctt tgatattcaa gatctgatta aaacttcacc gaatttagtt ggcgtagcag
     9781 gcagaatgga atgtatcacc gctccaaata aaccaatggt gattgtggac tatgctcaca
     9841 ccccggatgc tctcgaaaaa gccctacaag cggctcgttt acattgcgaa ggcgaacttt
     9901 ggtgcatttt cggctgtggc ggcgaccgtg acgccggcaa acgcccatta atggcgacga
     9961 ttgccgagaa attaaccgat aaagtgattg ctacagacga caaccctcgc accgaagata
    10021 atcaaaagat tatggcagat attgtgaaag gttttagtaa accccaacaa attatccaca
    10081 atcgtgaaga ggcaattaaa accgccattg agcaagctaa agccacagat gtgattctaa
    10141 tcgcaggcaa aggtcacgaa aattatcaga ttattggcac agagaagtta catttttcgg
    10201 atcaggaaac ggcaaggaaa tatctgtttt
//
LOCUS       DANKBV010000078        10121 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.078, whole genome shotgun sequence.
ACCESSION   DANKBV010000078 DANKBV010000000
VERSION     DANKBV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.078"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..424
                     /gene="ampD"
                     /locus_tag="PWY43_001616"
     CDS             <1..424
                     /gene="ampD"
                     /locus_tag="PWY43_001616"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDL2136484.1"
                     /translation="DLFFQGKLEPTAHPYFEEIKDLRVSAHCLINREGEITQYVNFND
                     MAWHAGVSCFEGREKCNEYSIGIELEGSNNQPFTQAQYQSLANLTKAIQQSYPQITDE
                     RITGHCHIAPERKIDPGQYFDWAYYKHLLNEAKKSAQQ"
     gene            complement(402..1070)
                     /gene="rluA"
                     /locus_tag="PWY43_001617"
     CDS             complement(402..1070)
                     /gene="rluA"
                     /locus_tag="PWY43_001617"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDL2136485.1"
                     /translation="MALIEYNPPLEPFLDEVYRDNHILVINKPSGLLSVPGNRPEYYD
                     SAMTRVQQKYGFTEPAHRLDMATSGILLFALSKAAEKELKRQFREREPKKHYIALLWG
                     KLGEKVGDKGEMNFPLICDWENRPRQKICYERGKKAITHYEVLAHYPNNIARVKLTPI
                     TGRSHQLRVHSLALGHPIIGDKFYANPLAKSLSPRLCLHAESLTITHPITRELMTFTA
                     EPTF"
     gene            complement(1082..1324)
                     /locus_tag="PWY43_001618"
     CDS             complement(1082..1324)
                     /locus_tag="PWY43_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDL2136486.1"
                     /translation="MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFP
                     NWMVEALDKQARHLGVPRQALSKMWIAEKLPVQSPR"
     gene            complement(1308..1604)
                     /locus_tag="PWY43_001619"
     CDS             complement(1308..1604)
                     /locus_tag="PWY43_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL2136487.1"
                     /translation="MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKV
                     VLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRLISVRRSRKKEISYYEKYH"
     gene            complement(1731..4643)
                     /gene="rapA"
                     /locus_tag="PWY43_001620"
     CDS             complement(1731..4643)
                     /gene="rapA"
                     /locus_tag="PWY43_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDL2136488.1"
                     /translation="MFLVGQRWLSESENNLGLGIVTSVDHRTVTINFPAAEEERVYAI
                     AVAPLTRVQFQAGDRINSIDGWQLEVSEVVENQGAVIYLGKRVDNGEEAVLPEMLLDH
                     KISFSKPQDRLFSAQIDRSDRFALRFNALQHQQAQYQSPLRGLRGIRASLIPHQLHIA
                     KEVGQRLAPRVLLADEVGLGKTIEAGMILQQQLFSGRAERVLVLVPESLQHQWLVEML
                     RRFNLKFSLFDEERCSDFDKQDENGDDVSENPFESEALVITSLNWLETSPQRANQVLA
                     ADWDLLIVDEAHHLEWSETESSIGYQLVERLSQQIPAVLLLTATPEQLGQESHFARLA
                     LLDSDRFYDYAEFVQEQAQYKPVADAVSSLLSDKPLSNDEQNAIAELLSEQDTEPMFR
                     AINSKKSDENDRLQARQELISELIDRHGTSRVLFRNTRQGVKGFPHREYHQISLEMPK
                     QYQNALKVMGMMGSVSQSDLLYPEYLFQRMNPNAKWAEFDPRIDWLITFLKNHRDEKV
                     LVICKQADTAIALEQVLREREAIRSAVFHEKMSIVERDKAAAYFAQQEEGAQVLISSS
                     IGSEGRNFQFAKDLVLFNLPDNPDLLEQSIGRLDRIGQKNDIQIYVPCFNLSAQQMLA
                     EWYHKGLNAFEETCPMGTAIFRELGEELQQFLQKSELSAQFDDFVAKTYKRQQYLKAE
                     LEKGQDRLLELNSNGGEAAQQLANDIAKEDNNPQLVNFALSLFDVIGVEQEDLGEQSI
                     VISPTGHMLVPDFPGLSEDGTTITFNRELALMREEVEFLTWDHPMIRNGIDLITSGDI
                     GKSAISLLVNNKLPAGTLLLEALYVVEAQAPKGLNLTRFLPPTPIRVLVDNKGNNIAP
                     QVSFAGLERQLKPVNKQMANKIAKMARADIEKLIKASEKAVEPQVQQVIETAKFEANS
                     KLKAELHRLESLKMVNKNIRADEVEALEQQLNASLAQLETANYRLDSLRVIVSNKA"
     gene            complement(4804..5103)
                     /locus_tag="PWY43_001621"
     CDS             complement(4804..5103)
                     /locus_tag="PWY43_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136489.1"
                     /translation="MRFLPSEIYQPRGELVKADRQGNGEFEVEYRVSGNDVRGLAKSA
                     IAHAKRKGFHLVESDIHRDDADLKFKRGDQELDIEIEVKGRNRIEYKADLDLDKN"
     gene            complement(5224..6549)
                     /locus_tag="PWY43_001622"
     CDS             complement(5224..6549)
                     /locus_tag="PWY43_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HDL2136490.1"
                     /translation="MISSVWNERYLADKKREQDHRSPFQRDRARLLHSEAFRCLQAKT
                     QIHAVGEDDFYRTRLTHSLEVAQIGSSLREKLLADVEGFQAVCSEQEFAKNMANLTAL
                     LPDHSLVESLCFAHDLGHPPFGHGGEMALNYKMYAFGGFEGNAQSFRIMTQLEPYTET
                     AGMNLTRRTLLGVMKYPALLSQTSPIEPAEMSEARHINLHHFPQSKGIFDDDNHFFEW
                     VLAPLSEKDRRQFCTVQPNQDPKQPNKTQYKSLDCSIMELADDIAYGVHDLEDAIVGG
                     MVTPQSWQNAEKLLAECQSDWVKQRLPEIREKLFSQHRYERKDVIGALVNHFITNVRW
                     KALPEFDEPLLRYNAYLPESVACVLKILKDFVYQYVICDAKTQRVERKGQRILMELFD
                     ILSSDPMRLLPNNVRERWKNAEESKRARVICDYLAGMSDGQAFKLFESL"
     gene            6634..7953
                     /gene="rlmD"
                     /locus_tag="PWY43_001623"
     CDS             6634..7953
                     /gene="rlmD"
                     /locus_tag="PWY43_001623"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2136491.1"
                     /translation="MAIFYSEKSAKKPIKSPACQKATIQSLDYQGLGIAKINGKTWFI
                     ENALPDEQVEFKVLEEKRQYGRGQAVKILKKSANRRMPSCELYGQCGGCQMQHISLEL
                     QRKAKQQALFQRLQKLQTHAIDVQPMIIGNDKHYRRRAKLSIAIQQGKAVIGFRLQNS
                     NQIIPLAHCEVLVEPLANLLPKLKHLIENWQQPKKLGHIELVSADNTISLFFRHLGAL
                     SAQDNHNLRQFATQEQLSLFVMTSENHIEHLCGERPYYQIQGLKLHFSIRDFIQVNAT
                     LNEKMVEKALEWLELSNQDRVLDLFCGMGNFTLPIAERAKSVVGVEGVEPMVQQAREN
                     AVTSGLKNVEFYQTNLDEPFSDKPWAKEPFNKVLLDPARNGALFCLDHLTSLNPERIV
                     YVSCNPATLVRDAEKLLQLGYRLEKAAMIDMFPHTGHLESISLFLKS"
     gene            complement(8093..9274)
                     /locus_tag="PWY43_001624"
     CDS             complement(8093..9274)
                     /locus_tag="PWY43_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDL2136492.1"
                     /translation="MESRRPSLSFFIILGMMAMLPPLAIDMYLPSFLDIARDLAVSQE
                     KVQTTLAVFTLGFGAGQLFWGPMADSFGRKIIILIGLVGSAIAAYFLTQVQNIETFYL
                     LRLVQGLCAAAPAVVLGALVRDLFERNLFAKLMSVIMIISMLAPLLAPIVGGYIAKYF
                     HWHSIFYVLVAMGILCVVLISWKIPETLAPEKRLPLSFGKTLSNFAILLKHKPTLGYV
                     LIGGLTFAGIFCFLTSGSIVYIGLYGVSQENFGYFFGLNMIVMVTMTAINSKMVVKVG
                     SETMLRIALAMQLLAGIWLALVAIFDLGFWAMAIGIPLYIGMLSTIGSNASAAILEQF
                     PQMAGTANGVAGTARFGVASLVGAGLSHIAVTSAAPMLLAMALCVMLAGVVYYFLCYK
                     R"
     gene            complement(9264..9965)
                     /gene="rsuA"
                     /locus_tag="PWY43_001625"
     CDS             complement(9264..9965)
                     /gene="rsuA"
                     /locus_tag="PWY43_001625"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDL2136493.1"
                     /translation="MRLDKFIAENTGLTRSQAAKVLKSGVVTVNGNIEKSGAAKISQE
                     DEIYYEDQKLEWVEEGQYFMLYKPQGYICSHDDGEYPTVFQFFDYPLMTKLHTAGRLD
                     VDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAQRLAEGILLRGEKEP
                     TLPAQLEILDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDERLEEGE
                     FRSLTSEEIASFNGK"
BASE COUNT         2898 a         2415 c         1969 g         2839 t
ORIGIN      
        1 cgatctgttt ttccaaggta aactcgaacc aaccgctcac ccttattttg aagaaatcaa
       61 agatctacgg gtttccgccc attgtttgat taatagggag ggggaaatca cgcaatatgt
      121 gaattttaac gatatggcgt ggcacgccgg cgtctcttgt tttgaagggc gggagaaatg
      181 caatgaatat tcgattggca ttgaactgga aggcagtaat aatcagccgt ttactcaagc
      241 tcaataccaa tctttggcaa atttgaccaa agccattcag caaagctatc cacaaattac
      301 cgatgaacgc atcaccgggc attgccatat tgccccggaa cggaaaatcg acccggggca
      361 atattttgat tgggcatatt acaaacacct actgaacgag gctaaaaagt cggctcagca
      421 gtaaacgtca tcaactccct tgtaatcggg tgagtaattg ttagtgactc cgcgtgcaaa
      481 cataaacggg gcgataaact ttttgccagc ggattagcat aaaacttatc gccaataatc
      541 gggtgaccta acgcaaggct atgtactcgt agctgatgcg aacgccccgt gataggcgtg
      601 agttttactc tcgctatatt attcggataa tgagctaaca cttcataatg ggtaatggct
      661 tttttgcctc gttcatagca aattttctgg cgggggcgat tttcccaatc gcaaatcagc
      721 ggaaaattca tttcgccctt atccccaact ttttcaccta atttgcccca aagtaacgca
      781 atatagtgtt ttttcggttc acgttcccga aattggcgtt ttagttcttt ttccgccgct
      841 ttgcttaggg cgaacaaaag aataccgctt gttgccatat ccaaacgatg agccggttcg
      901 gtaaagccat atttttgctg tactcgagtc attgcactgt cgtaatattc gggacgattg
      961 cccggcaccg ataataaacc actaggctta ttaattacta aaatatggtt atcccgataa
     1021 acctcatcta aaaatggttc taatggaggg ttatattcaa ttaatgccat ttgccactcg
     1081 cttacctagg tgattgaacc ggtaattttt ccgcaatcca cattttactc aaggcttggc
     1141 gaggcacacc taaatgtctg gcttgcttat ctaacgcttc taccatccaa tttgggaaat
     1201 caacattcac ccttttcatt tgccccgggc gatgagcggt agaaagatcg cagtattgta
     1261 aaatatcttc tttgccttca tcgaagatac gttctaattc ttcggcacta atggtatttc
     1321 tcatagtatg agatctcttt tttacgggaa cgtctaactg aaattaaacg aataacatca
     1381 tttcttggtg taaaaaacgc tgtccaatat ttatcataaa tcttgccaat caggaaatag
     1441 cgtaattcgg gttcaatgca agcctccagt acaacttttc gttcatcttc ccaaagcaac
     1501 tttgcctgtt caaaatcaat accatatttg gctaaattaa tttggctttt gttcgggtcg
     1561 tattcaaaca ataacggcac agcatattgc aaatctatat acataatatt ccttttttat
     1621 accttttaca atatgaggaa tagcataaat aatctgcaag gatgacacaa aatagaaaag
     1681 tgattttttc gacatagatc gaaaaaatca cttttcgtac ttgcaaaaaa ttacgcttta
     1741 ttacttacaa tcactcgcaa gctatctaag cgataatttg ccgtttcaag ttgggcaagg
     1801 gaagcgttaa gctgttgttc taaggcttca acttcatcgg ctcgaatatt tttattcacc
     1861 attttgagtg attccaagcg gtgtaattct gcttttaatt tgctatttgc ctcaaatttg
     1921 gcagtttcaa tcacctgttg gacctgaggt tctaccgctt tttcactcgc tttaattaat
     1981 ttttcaatat ccgctcgagc cattttggca attttatttg ccatctgttt attcaccggt
     2041 tttaattggc gttcaagccc ggcaaaagag acctgcggag caatattgtt gcctttgtta
     2101 tccactaaaa cccgaatcgg ggttggcggt aggaaacggg ttaaatttag ccctttcggt
     2161 gcttgtgctt ccaccacata aagcgcctca agtaataatg taccggcagg caatttatta
     2221 ttcaccagca atgagattgc tgatttgcca atatcgccgg aggtgattaa atcaatacca
     2281 ttgcggatca tcgggtgatc ccaagtgaga aattccacct cttcccgcat taacgccagt
     2341 tcacgattaa aggtaatggt cgtgccatct tccgatagcc ccgggaaatc aggcacaagc
     2401 atatgtccgg ttgggctaat cacaatgctc tgctcgccta aatcttcctg ttcaacgcca
     2461 atcacatcaa acaaactcaa ggcaaaatta accaactgcg gattattatc ttctttcgca
     2521 atatcattag cgagctgttg agctgcttct ccgccattag aattcagttc taataatctg
     2581 tcctgtcctt tctccagttc ggctttcaaa tactgttggc gtttataggt tttcgcaaca
     2641 aaatcatcaa attgggctga aagttcagat ttttgcagga attgttgcaa ttcttcacct
     2701 aattcacgga agatcgctgt tcccatcggg caggtttctt caaacgcatt caagcccttg
     2761 tgataccact ctgccaacat ctgttgagcc gataaattaa agcaaggcac ataaatctga
     2821 atgtcatttt tctgtccgat acgatctaat cgcccaatac tttgctccaa taaatccggg
     2881 ttatccggca aattaaacag cactaaatct ttagcaaatt ggaaatttcg cccttctgaa
     2941 ccgatgcttg aactaatcag cacctgagcg ccttcttctt gttgagcaaa ataggcggca
     3001 gctttgtcac gttcaacaat cgacattttc tcgtggaaaa cggcagaacg aatcgcctca
     3061 cgctcacgca aaacttgctc caaagcaatc gcagtgtccg cctgtttgca gatcaccagc
     3121 actttttcat cacgatgatt ttttaagaaa gtgatgagcc aatcaatccg agggtcgaat
     3181 tcagcccatt tcgcattcgg gttcattcgt tggaacaaat attcaggata gagcaaatca
     3241 ctttggctca cactccccat cattcccatc acttttaacg cattttggta ttgtttcggc
     3301 atttctaggc tgatttggtg gtattcccga tgtgggaagc ctttcactcc ctgacgggta
     3361 tttctaaaca acacccggct ggttccatgg cggtcaatta gctcactaat cagctcttga
     3421 cgggcttgca agcggtcatt ttcgtccgat tttttgctat taattgcacg gaacatcggc
     3481 tcggtatctt gctcgcttaa cagctccgca atcgcattct gctcgtcatt acttagcggt
     3541 ttatcgctta ataagctact caccgcatct gccaccggtt tatactgtgc ttgctcctgt
     3601 acaaactcgg cataatcata aaaacgatcc gaatctaaca acgccaagcg ggcaaaatgg
     3661 ctctcttgcc ctaattgctc cggtgttgcc gttaaaagca agacggcagg aatttgttga
     3721 gataaacgct ctaccaactg atagccgata gaagattcgg tttccgacca ttctaaatgg
     3781 tgagcttcat caacaattaa tagatcccag tcggcagcaa gcacttgatt ggctcgttgc
     3841 ggtgaggttt ccagccagtt tagtgaagta atgactaacg cttcgctctc aaatggattt
     3901 tcacttacat catcgccatt ttcatcttgt ttatcaaaat cactacaacg ttcttcatcg
     3961 aataacgaga atttcagatt aaaacggcga agcatttcca ccaaccattg gtgttgcaaa
     4021 ctttccggca ctaataccaa tactcgttct gctctgcccg aaaatagctg ttgttgcaaa
     4081 atcataccgg cttcaatagt tttacccagt cccacttcat cggctaatag cacacgagga
     4141 gcaaggcgtt gcccgacttc ttttgcaata tgtaattgat gtggaatcag gctggctcga
     4201 atgcctcgca aaccacgcag tggcgactga tattgcgctt gctggtgctg caaagcgtta
     4261 aagcgtaatg caaagcgatc tgagcgatca atctgagccg aaaaaaggcg gtcttgcggt
     4321 ttgctgaaac tgattttatg atcgagcaac atttcaggca gtaccgcttc ttcaccgtta
     4381 tccacccttt tgcccaaata gattacggca ccttgatttt ccacaacctc tgaaacctct
     4441 aactgccagc catctatact gttaattcta tctccggctt gaaactgcac tcgagttaaa
     4501 ggtgcaactg caattgcata gacccgctct tcttccgctg ccgggaaatt gatagtgaca
     4561 gtgcgatgat ccaccgaagt cacgattccc aaacctaaat tattttccga ttcacttagc
     4621 caacgctgac cgactaaaaa cattttttcc tctaagtaat aagtaaaagc atcattattg
     4681 tcatttaaac ggctatttta ccaaattccg ggaccggtcg gcttgagtat ttttgcaaaa
     4741 aagcacaata aaaagaccgc ttgtgcggtt aaatcacaca agcggtcgtt ttaaggggta
     4801 aatttagttt ttatctaaat ctagatctgc cttatattca atccgatttc taccttttac
     4861 ttcaatttca atatccagct cttggtcacc acgtttgaat tttaaatcag catcatctct
     4921 atgaatatca gattctacta aatggaaacc tttacgttta gcgtgggcga tcgcactttt
     4981 cgctaagcca cgaacatcat taccactgac acgatattct acttcaaact caccattacc
     5041 ttgacgatct gctttaacta attcgccacg aggctgataa atttcacttg gaagaaaccg
     5101 caaatgctgt tgcagaagcc aaagttgcta atactaaaag acctttagaa acagttttca
     5161 ttgaaatctc ctattaaata aatgaaacct attactgaga ccttgcaaaa ttaacaaagt
     5221 tcattataaa ctttcaaaaa gtttaaacgc ctgcccatca gacataccgg ctaaatagtc
     5281 gcaaattacc cttgcacgct tagattcttc cgcatttttc caacgttccc gcacattatt
     5341 agggagtaat cgcatcggat cactgcttaa aatatcgaac aattccatta gaatgcgttg
     5401 ccccttgcgt tccacccgct gggttttggc atcacaaatc acatattgat acacaaaatc
     5461 tttcaagatt ttcagcacac aggcaacact ttccggtaaa taggcattat aacgtagcaa
     5521 cggctcgtca aattcaggca gagctttcca acgcacattg gtaataaaat gattcactaa
     5581 tgccccgatc acatcttttc gctcgtagcg gtgctgagaa aagagctttt cccgaatctc
     5641 cggcaggcgt tgtttcaccc aatcggattg gcactccgcc agcaattttt ccgcattttg
     5701 ccacgattgc ggtgtgacca ttccacctac aatcgcatct tccaaatcgt gtacgccata
     5761 ggcaatatcg tccgctagtt ccattatgct gcaatcgaga gatttatatt gcgttttgtt
     5821 tggctgtttc ggatcttgat tgggctgaac cgtgcaaaat tggcgtcggt ctttttcact
     5881 taacggggct aatacccact caaaaaagtg gttgtcgtca tcaaaaatcc ctttgctctg
     5941 tggaaaatgg tgcaaattaa tatgccgagc ctcgctcatt tctgccggct caatcggcga
     6001 ggtttgagac aagagtgccg gatatttcat tacccccagt aaggtgcgac gggtcagatt
     6061 cattccggct gtttcggtgt aaggctcaag ctgggtcata atccgaaagg attgagcatt
     6121 accctcgaag ccgccaaagg cgtacatttt ataatttaac gccatttcac caccgtgccc
     6181 gaacggaggg tgcccgagat cgtgggcaaa acaaagggat tccaccaagc tatgatccgg
     6241 caataaagcg gtcagatttg ccatattttt tgcaaattcc tgctccgaac agaccgcttg
     6301 aaagccttcc acatccgcta ataatttttc tcgcaaactg ctaccgattt gagccacttc
     6361 caacgaatgg gttaaacggg ttcgataaaa atcatcttcc cccacggcgt gaatttgcgt
     6421 tttcgcctgc aaacagcgaa aagcctccga atgcaataac cttgcccgat cccgctgaaa
     6481 aggcgagcgg tgatcttgtt cccgtttttt atccgccaaa taacgttcat tccaaaccga
     6541 tgaaatcata ttgcctccat tttccgccaa ctgcttttct tatataatct ccccatttcg
     6601 acactttata tcataacaat tcatctgaaa acaatggcga ttttttattc tgaaaaatcg
     6661 gcaaaaaaac cgataaaatc acccgcttgt caaaaggcaa ctattcaatc gttagattat
     6721 caaggcttag ggattgccaa aatcaacggc aaaacttggt ttatcgaaaa tgccctgcct
     6781 gatgagcagg tggaatttaa agtgttggaa gagaaacgcc aatacggacg tggacaagcg
     6841 gttaaaattc ttaaaaaatc tgcaaatcgg cgaatgccaa gctgcgaact ttacgggcaa
     6901 tgcggtggct gccaaatgca gcatatttcg cttgaactgc aacgtaaggc aaaacaacag
     6961 gctttattcc aacgcttaca gaaattgcaa acccacgcta ttgatgttca gccgatgatt
     7021 atcggcaacg ataaacacta ccgccgtcgg gcaaaattaa gcattgcgat ccagcaaggc
     7081 aaggcggtaa tcggctttcg tttgcaaaat tccaaccaaa ttataccgct tgcacactgt
     7141 gaagtgctag ttgaaccgct tgccaatttg ctcccaaaat taaaacacct gattgaaaac
     7201 tggcaacagc cgaaaaaatt ggggcatatt gagctggtca gtgcggacaa taccatttcc
     7261 ctatttttcc gccatttagg ggcattatcg gcgcaagata accacaattt gcgtcaattc
     7321 gccacacaag agcaattatc tctttttgtg atgactagcg aaaatcacat tgagcatctg
     7381 tgcggtgaac gcccgtatta tcaaattcag ggtctaaagt tgcatttttc aattcgggat
     7441 tttattcagg tgaatgccac gctcaatgaa aaaatggtgg aaaaagccct tgagtggctg
     7501 gaattatcca accaagatcg agtgttagat ctcttctgtg gaatggggaa ttttaccttg
     7561 cctattgctg agcgagcgaa atcggtggtc ggtgtggaag gggtagaacc aatggtgcaa
     7621 caagcccgag agaatgcggt gacaagcggt ctaaaaaatg tggaatttta ccaaaccaat
     7681 ttagatgagc cgttttctga caaaccttgg gcaaaagagc cgtttaacaa ggtactactc
     7741 gacccggcac gcaacggagc actattttgc ctagatcact tgacatcact caatcctgag
     7801 cggattgttt atgtctcttg caatccggca acactggtgc gagatgccga aaaactgttg
     7861 caactaggct acaggctaga aaaagcggca atgattgata tgttccccca caccgggcat
     7921 ttggaatcta tttcgctgtt tttaaaatct tagtagatat tgccccgatg gtgcgagcgt
     7981 ccacgctcgt accacacaag cggttaattc tgcctaaaaa tttgcaaaaa atgtactatt
     8041 attaaccgct tgtagaaatt tgttgggttt tcgcaagcta accccaacct agttatcgct
     8101 tataacacag aaaataatac accacaccgg ctaacataac gcacaatgcc atcgccaaaa
     8161 gcattggggc agcactggtt acagcaatat gtgaaagccc tgctccaaca agggaagcaa
     8221 caccaaaacg agccgtacct gctactccgt tagccgtgcc tgccatttgt ggaaattgct
     8281 ctaaaatggc ggcactggca ttactaccaa tggtggaaag cattccgata taaagcggaa
     8341 taccgattgc cattgcccag aagcctaaat caaaaatcgc cactaaagcc agccaaatgc
     8401 cggctaacag ttgcatagct aaagcaattc gtaacatcgt ttcagagccg actttcacta
     8461 ccattttgct gttaatcgcc gtcatcgtca ccatcacaat catattcagc ccgaagaaat
     8521 agccgaaatt ttcttgcgac acgccataaa gcccaatata gacaatcgag cctgaggtta
     8581 aaaaacagaa aatacctgca aaggttaagc cgccaatcag cacataacca agagtaggct
     8641 tatgtttcag taagatggca aaattgctta aagttttgcc aaaacttaac ggcaggcgtt
     8701 tttcgggggc taatgtttca gggattttcc aactaattaa taccacgcac aaaatcccca
     8761 tcgccactaa cacatagaaa attgagtgcc agtggaaata tttggcaata taaccgccaa
     8821 caatcggtgc aagcaatggg gcaagcatag aaataatcat aataaccgac atcaatttgg
     8881 caaataaatt acgctcaaac agatctctca ctaatgctcc cagcaccacc gccggggctg
     8941 ccgcacataa gccctgtact aaccttagca ggtaaaatgt ttcgatattc tgaacttggg
     9001 tcagaaaata agcggcaatc gccgagccga ctaatccgat taaaataatg attttccgcc
     9061 caaagctgtc tgccatcggt ccccagaata gttgccctgc cccaaaacct agggtaaata
     9121 ccgccaaggt ggtttgcact ttttcttgcg aaaccgccaa gtcacgggca atatctaaaa
     9181 atgagggcaa atacatatca atggctaatg gtggcaacat tgccatcata cctaatatga
     9241 tgaaaaagct caggcttggt cttctacttt ccattaaaac tggctatctc ctctgaagtg
     9301 agcgagcgga attcaccttc ttccaacctt tcatctaaca ccacatcgcc aattcgccag
     9361 cggtgcaatg cctcgacttt attcccaagg gcggcaaaca ttcgcttaac ttgatgataa
     9421 cgcccctcac taatagttaa attcacattg taatcgtcta aaatttcaag ttgagccggc
     9481 aaggttggct ctttctcgcc acgcaataaa attccttctg ccaatcgctg tgcataaaaa
     9541 tcttccaccg gatcagcaag cgtcactaag tatgtctttt cacaatggtg cttgggcgag
     9601 gtaatacggt gcgaccattt gccgtcatcg gttaacagca ccagccctgt ggtatccaca
     9661 tctaaccgcc ctgcggtatg caatttagtc attaacggat aatcaaagaa ttgaaacacc
     9721 gttgggtact caccatcatc gtgtgaacaa atatagccct gtggcttata gagcataaaa
     9781 tactgcccct cttccaccca ctctaatttc tgatcttcgt aataaatttc atcttcttga
     9841 ctgatttttg ccgcaccact tttttcaata ttgccgttta ccgtcaccac accacttttc
     9901 agcactttag ccgcttgtga acgggtcaaa ccggtatttt cggcgataaa tttgtctaaa
     9961 cgcatactat ttttatacca taaattactt taccctctcc cctcgtggga gagggacaga
    10021 tttttcttcg tctagaagaa aaatcaggga gaggggggaa atacgtcttc gtccagaaga
    10081 aaaatcaggg agaggggaaa tgtgcaaact tttttagaaa a
//
LOCUS       DANKBV010000079        10029 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.079, whole genome shotgun sequence.
ACCESSION   DANKBV010000079 DANKBV010000000
VERSION     DANKBV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.079"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..298)
                     /locus_tag="PWY43_001626"
     CDS             complement(<1..298)
                     /locus_tag="PWY43_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2136494.1"
                     /translation="MRKAQRKTIRLKYYDYAQNGLYFVTICVQGRLSLFGGVLDNAMY
                     LNDAGKMVEKQYKELENYFNNVTCLEYIIMPNHIHFIIQILNVNVIQQSLFDIIQ"
     gene            complement(556..2748)
                     /gene="yccS"
                     /locus_tag="PWY43_001627"
     CDS             complement(556..2748)
                     /gene="yccS"
                     /locus_tag="PWY43_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDL2136495.1"
                     /translation="MNKFLRYLQHSWLSENVIKTLPMFISLNIAAVSIWQLGISDFAM
                     PLMLGIIAGGLVDLDNSLIGRAKNLIISLVAFSLSSIGASVSLHLGWLFVPVAVVCTF
                     LLVMLGAIGQRYSTIAFGTLIVAVYNSLAYTPEVLWYHNVVMILLGTLLYGLVGILVY
                     LIFPNRTVQENLASAYLALSHYLLAKSAYFDPDDDDLAAKQLALAKANSEVMAAFDKT
                     RVALFYRLRRQHKQQRTQRMLRYYFTAQDILERASSSHYQYHELFQQLSNSDLMFRFQ
                     RVLELQAAACQKIAMSLRHREVYEHGMCGEKALQGLLNSLNYHQERGLKSAYRWRAIA
                     ENLGNIERQLAQIEQGSSSGEHIEPLSKNFTKSHRLIAENITGLSNISQAIKAHLTFE
                     SQLFRHAVRLSIVVLLCSAIVPILGFDSKGYWILLTAIFVCQPNYTATKTRLIQRVIG
                     TIFGVLVGGSFELFNVTLSLEAQLGLIVLTGSLYTFFRLVHYGFSTFYITVLVMISLD
                     IAGVSLESGILMRMLDTLLGTAIAWLAVAFLWPDWKYLNLQRNLHNSLKASGQYLRHI
                     IAQLQFGYNEQLPYRIARRDVHNHLSALSNAITNMYSEPQKYANDLTFAPKLLSIAYT
                     LLGYISTLGAYRIASREINQQVDFSAMFFSLAREVANIIGQIASESRSFAKLSEKLTA
                     IDQDLAQFEQENQQKQNDLSLVLIQQLRLIVQLLPQMQSLLKAEICHQ"
     gene            complement(2792..3250)
                     /locus_tag="PWY43_001628"
     CDS             complement(2792..3250)
                     /locus_tag="PWY43_001628"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL2136496.1"
                     /translation="MKTTVRTIAKQKQIALVAHDSCKQDLIRWTQTHQAQLASHKLYA
                     TGTTGHLLARETGLEITSLLSGPMGGDQQLGGLIAEKKIDLLVFFWDPMNAAPHDPDV
                     KALMRIATVWNIPVAINETTANFLLNADLFHQEVSVNVPDYDGYLAERLG"
     gene            3376..4650
                     /gene="thrC"
                     /locus_tag="PWY43_001629"
     CDS             3376..4650
                     /gene="thrC"
                     /locus_tag="PWY43_001629"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDL2136497.1"
                     /translation="MNLYNIKHPEEQVNFAQAVRQGLGKNQGLFFPEVLPKLGSIDEL
                     LDLPLVERSQKILSALIGEELPQETLNAMVKNAFTFPAPLAKVNDDIYALELFHGPTL
                     AFKDFGGRFMAQALANVRGDGKITILTATSGDTGAAVAHAFYGLENINVVILYPKGKI
                     SPLQEKLFCTLGGNIRTVAIESDFDACQALVKQAFDDAELRQAIGLNSANSINISRLL
                     AQICYYFEAVAQLPKEKRDNVVVSVPSGNFGNLTAGLIAKTLGLPIKRFIASTNANDT
                     VPRYLQSGKWEPNATVATLSNAMDVSRPNNWPRVEELFKRNGWSLSELGSAALNDAET
                     EAALKAQYAEGYLCEPHGAIAYQSLKDQLQQGETGIFLCTAHPAKFKESVERILAIEL
                     PLPEALDKHNKLPLLSDEMAADFATLRAYLLK"
     gene            complement(4729..5463)
                     /locus_tag="PWY43_001630"
     CDS             complement(4729..5463)
                     /locus_tag="PWY43_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL2136498.1"
                     /translation="MSFKDMTGQKVPSVTFRTRQNDAWVDVTTDELFNNKTVVVFSLP
                     GAFTPTCSSTHLPRYNELACEFKALGVDDIICMSVNDTFVMNAWKADQESENITVIPD
                     GNGEFTEGMGLLVDKNDLGFGKRSWRYSMLVKNGIVEKMFIEPQEPGDPFKVSDADTM
                     IKYLSPNWTAKESVSIITKPGCPFCAKAKALLKEQGYTFEEIVLGRDASTISVRAITG
                     KTSVPQVFIGGKHIGGSDELAAYFAK"
     gene            5578..6462
                     /gene="oxyR"
                     /locus_tag="PWY43_001631"
     CDS             5578..6462
                     /gene="oxyR"
                     /locus_tag="PWY43_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDL2136499.1"
                     /translation="MNIRDLEYLIALAEHKHFRRAAESCNVSQPTLSGQIRKLEDELG
                     TILLERTSRKVLFTQAGLTLVEQAKVVLREVKILKEMASNQGKEMSGPLHVGVIPTLG
                     PYISPIIVPALKAEFADLELYIYELQTNQLLDQLESGQLDCGIISFVKESESFIEVPI
                     FNEEMYLVVPEQHEWAALKKFDMNLLKNRELLFLDSGHCLRTNTLDYCLSIGAKENPH
                     FKATNLETLRNMVAANVGLALIPKLAAKPIKGVHYIPLENPKPFRAVGMVYRPGSPLR
                     ARYERLAQAITKIMQSEA"
     gene            6465..7097
                     /gene="fabR"
                     /locus_tag="PWY43_001632"
     CDS             6465..7097
                     /gene="fabR"
                     /locus_tag="PWY43_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDL2136500.1"
                     /translation="MANGMSVRAIQKEKTRRALVEAAFNQLSSEKNFSNLSLREVARE
                     AGVAPTSFYRHFKDMDELGLAMVDESGLLLRQLMRQARKRIANGGSVVVVSVETFFEL
                     IADRPNVFQLLLRESSGTSQAFRTAASREIQHFVAELADYIMSKDNHSNRELAYIQSE
                     GLVTLVFTAGSHALDMNNQEREKLKQRVIMQLRMLIRGSTYYANKQASST"
     gene            complement(7124..7423)
                     /locus_tag="PWY43_001633"
     CDS             complement(7124..7423)
                     /locus_tag="PWY43_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HDL2136501.1"
                     /translation="MKKADIQKMQDCYNQWVELLPELEKGIEQWKRASALLEPLSRFY
                     ASPEWRELHDSFDEQLDTKGNYSILSEDALWNALSEHHQLALEWLKLSTAYITKE"
     gene            complement(7512..>10029)
                     /locus_tag="PWY43_001634"
     CDS             complement(7512..>10029)
                     /locus_tag="PWY43_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL2136502.1"
                     /translation="NVAAGDVNADSKDAVNGSQLYTFAMASREEVKSTDKSVTVNTTK
                     NADGANVFDLSVNTDDVTIVKDPTTGAIKANTTALNDANNDGRIDEPTADDAKKLVTA
                     GDITNAINNSGFTLKTSAVEGGEKLSGGDELINPGKAVEMVAGKNLTVKQEADGKVIY
                     ATKDDVKFSSVTSNTVTVPTDEADPANNPITINKDGINAGNKAISNVASNLTPVTADD
                     KVQPADNNPTNLADKLSNAATLGDVLNAGWNLQGNGKAVDTVVHNDTVDFINGKGTTV
                     TVENKDGKNTIKVDSPIEFVNQDPTDSSTPSNTAKFTGEAPVQLGNVASSVRNEDGST
                     PEGKDRAEAIKNAEGDKLNNVVNLGDLQAATNAATTKVGGNRGVTITPSTNADGSTTY
                     NVEAKTDGTTIKVDNEGNITANTSELGNNEDGTVKAPTQPNALVTAQTVADAVNNAGF
                     NIKSAGNKAAGDQAATKLVKTGEEVVFEAGDNLTVKRDGNQFTFATAKDVSFNSVQFS
                     ENGPKITNDGDNIKVGDKDGKPTKITNVADGDISPVSTDVINGKQLNNYAKVNGNNIG
                     TDEDGSINIVNGNGTTITSDKAGEVKVNVNSTDLTVADNGKINVQDPNGTGSHFVNAT
                     TVANAVNNVSWNVDSKAVGTGVVEGDKAPAKVKAGSTVSVNAGNNIKVTRKGSDVTVA
                     VSDTPEFTSVKTGDTLVNNNGVTINNGSAGKAVSLTKDGLNNGGNRITNVKAGEADTD
                     AVNVGQLKGAVNHLNNKIHRNNREARAGIAGSNAAALPQVYIPGKSMVAAAGGTFKGE
                     NALAVGYSRSSDNGKLILKLQGNANSRGDFGGGVGVGYQW"
BASE COUNT         2920 a         2257 c         1876 g         2976 t
ORIGIN      
        1 gttgaataat atcaaataga gattgctgaa taacattcac attaagaatt tggattatga
       61 aatgaatatg gtttggcata attatatatt ctaaacaagt gacattatta aaataattct
      121 ctaattcttt atactgtttt tctaccattt tacctgcatc attcaaatac atcgcattat
      181 caagaacacc gccaaataaa gataaacgcc cttgaacaca tattgtcacg aaataaagac
      241 cattttgtgc gtaatcataa tactttaaac gaatagtttt gcgttgagct tttctcatct
      301 ttaatttcct aaataaattt attatttaat tagaaatgat taacatgcaa tactataaaa
      361 cgtttttttc gagcaagatc acaaaaagat taataatatt tattaaatta tttactaatg
      421 gttatttagg aaaattacgg acgggcacag gacccgtccc tacgctaaat ttttatcaca
      481 taagaaatcc tactacaaat tatataatta atacaaccgt aggggcgggc cctgtgcccg
      541 cccgtgcttc ttctattact gatggcaaat ttcagctttc agcaagcttt gcatctgcgg
      601 taacaactgc acaatcagcc tcagttgttg tatcaacact aaggaaagat cattttgttt
      661 ttgctgattt tcctgctcaa actgggctag gtcttggtcg atagcggtca atttttccga
      721 taattttgca aatgaacggc tctctgaggc gatttgccca atgatgtttg ccacctctct
      781 ggcgagtgag aagaacattg cggaaaaatc cacttgttga ttaatctctc ggctagcaat
      841 acggtaagcc cctaaggtgg aaatataacc cagcaaggtg taagcaatac ttaacaattt
      901 cggggcgaag gttaaatcgt tcgcatattt ttgtggttcg ctgtacatat tggtaattgc
      961 attactgagt gccgataaat ggttatgcac atcacggcgt gcaatgcggt aaggtagttg
     1021 ctcgttataa ccaaactgca actgggcaat aatatggcgt aaatattgtc cactagcttt
     1081 caagctatta tgtaagttgc gttgcaaatt taaatatttc caatccggcc ataagaatgc
     1141 taccgccaac cacgcaatcg cagtgccgag caaagtatcg agcattcgca ttaagatgcc
     1201 gctttcaagg ctcacgcctg caatatcaag gcttatcatt accaacaccg taatataaaa
     1261 ggttgaaaag ccataatgta ccaagcggaa aaaggtatag agcgagccgg tcagtacgat
     1321 tagcccaagc tgggcttcca gactgagcgt tacgttaaac aattcaaaag agccaccgac
     1381 cagcacgccg aaaatcgtgc cgattacacg ctgaatcagt cgggtcttag tggcggtgta
     1441 attcggttgg cagacaaaaa tcgccgtgag caaaatccag taacctttgc tgtcgaagcc
     1501 taaaatcggc acgatcgcac tgcacaacag caccactatc gacagccgaa cggcgtggcg
     1561 aaacagttgg gattcaaagg ttaaatgggc tttaatcgct tggctgatat tgcttaagcc
     1621 ggtaatattt tcggcgatca agcggtgaga tttggtgaaa tttttgctta acggctcaat
     1681 atgctcacca ctgcttgagc cttgttcaat ctgagctaat tgccgttcaa tattgcctaa
     1741 attttctgca atagcacgcc aacgataggc acttttcaag cccctttctt ggtgataatt
     1801 taatgagtta agtaacccct gcaaggcttt ttcgccacac ataccgtgtt catatacttc
     1861 cctatggcgt aacgacatcg caattttttg acacgccgcc gcctgtaatt ccaatacccg
     1921 ttgaaagcga aacattaaat cactgttgct taattgttga aacagctcgt gatattgata
     1981 atggctggag cttgcccgct ctaaaatatc ttgagcggtg aaatagtaac gcagcattcg
     2041 ctgtgtccgt tgttgcttgt gctgacgtct taggcggtag aatagggcga ctctggtttt
     2101 atcaaaagca gccatcacct cgctattggc tttcgccaaa gctaactgtt tcgcagctaa
     2161 atcgtcatca tcggggtcaa aataggcaga tttcgccagt aaatagtggc tcaatgcaag
     2221 gtaggcggac gctaaattct cctgtacagt gcgattcgga aaaatcaggt aaaccaaaat
     2281 accgaccaaa ccatagagta aggtgccaag taagatcatc accacattgt gataccacaa
     2341 tacttcgggc gtataagcca ggctgttata aaccgccaca atcagcgtgc caaaagcaat
     2401 ggtactatag cgttgcccga ttgcccctaa cattaccagc aaaaaggtgc aaaccaccgc
     2461 caccggcaca aacagccagc ccaaatggag cgacacactc gccccaatcg aggaaagcga
     2521 gaacgccact aggctgataa tcagattttt cgctcgtccg attaggctgt tgtctaaatc
     2581 caccaaaccg ccggcgataa tgcctaacat caacggcatc gcaaaatcag aaataccgag
     2641 ttgccaaata ctaaccgctg caatattcag cgaaataaac ataggcagcg ttttaattac
     2701 attttccgaa agccaactgt gttgtagata tcttaaaaat ttattcatat ttgctcattt
     2761 cgggcaggca cagaacctgc ccctacctac actaccctaa acgttctgct aaatagccat
     2821 cataatccgg cacattcacg ctgacttctt gatgaaacaa atccgcattt aacagaaaat
     2881 tagcggtagt ttcgttaatc gccaccggaa tattccaaac ggtcgcaatc cgcatcaggg
     2941 ctttcacatc aggatcgtgc ggagcggcat tcatcggatc ccaaaagaac accagcagat
     3001 caattttctt ttctgcgatc agcccgccta attgctgatc gccccccatc gggccgctta
     3061 acaatgaagt aatttcaagc cctgtttctc tggcgagcaa atgcccggta gtgccggtgg
     3121 catatagctt atgcgaggca agttgggctt gatgagtttg tgtccaacga attaaatctt
     3181 gtttacagct atcgtgagcc actaaggcaa tctgtttttg ttttgcgatg gttctgacgg
     3241 tggttttcat ttttagctcc tttgcaaaat ttttcacaaa tcataccgct tatttttcgc
     3301 ttttgcattg actaatcacg gcttatggct aaaatcaggt aaaaattcgc attttaaaac
     3361 aaaggattaa ccgcaatgaa cttatacaac atcaaacacc ctgaagaaca agtcaatttc
     3421 gcccaagcgg tacgccaagg cttaggcaaa aatcaagggc tatttttccc tgaagtgtta
     3481 cctaaattag gtagcattga tgaattattg gatttacctt tggttgaacg cagccaaaaa
     3541 attctttcag ccttaatcgg cgaagagtta ccgcaagaaa ccttaaatgc aatggtaaaa
     3601 aatgcgttca ctttcccggc accgcttgcc aaagtcaatg atgatattta tgcgttagaa
     3661 ttatttcacg gtccgacttt agccttcaaa gattttggtg ggcgctttat ggctcaagcc
     3721 ttagccaatg tacgaggaga cggcaaaatt actattttaa ccgctacctc aggtgacacc
     3781 ggtgcggcgg tggctcatgc tttctatggt ttggaaaata tcaacgtggt cattctttac
     3841 ccgaaaggca aaatcagccc attacaagaa aaattattct gtaccttagg cggtaatatt
     3901 cgcactgttg cgattgaatc cgactttgat gcttgccaag ccttagtgaa acaagccttt
     3961 gatgatgccg agctacgcca agcgattggt ttaaattccg caaattccat caacattagc
     4021 cgtttactgg ctcaaatttg ctactacttt gaagcagttg ctcagttacc aaaagaaaaa
     4081 cgagataacg tagtggtttc agtaccaagc ggtaatttcg gtaacttaac cgccggctta
     4141 attgccaaaa cattaggttt accgattaaa cgttttattg cctcaaccaa tgccaatgac
     4201 accgtgccac gctatttaca atcaggcaaa tgggaaccaa acgcaaccgt tgcaaccctt
     4261 tctaatgcaa tggacgtaag ccgcccgaac aactggccac gagttgaaga attatttaaa
     4321 cgcaatggct ggagtttgtc tgaattgggt tctgccgcat taaacgatgc cgaaaccgaa
     4381 gcggctttga aagctcaata tgcagaaggt tatctgtgtg agcctcacgg tgctattgct
     4441 taccaaagct tgaaagatca gctacaacaa ggcgaaaccg gtattttctt atgcaccgct
     4501 cacccggcaa aattcaaaga gtcggtggaa cgcattttag cgattgaatt gccgttaccg
     4561 gaagcattag ataagcacaa caaactgcca ctgttatccg atgaaatggc agcagatttt
     4621 gcaacattgc gtgcatattt gttgaaataa atattcaata atagaaaagg gattaagtat
     4681 taaacttaat ccctcttttt atgcattaca agcggttata tttgcctatt attttgcaaa
     4741 ataagcagct aactcatcac taccgccaat atgtttaccg ccgataaata cttgtggtac
     4801 agaggtttta cctgtaattg ctcgtacaga aatcgtgctt gcatcacgac ctaacacaat
     4861 ctcctcaaaa gtatagcctt gctcttttaa taatgctttt gcttttgcac agaacgggca
     4921 acccggttta gtgataatcg aaacagactc tttagctgtc caatttggac ttaaatattt
     4981 aatcattgta tcggcatctg atactttaaa cggatcgccc ggttcttgtg gctcaataaa
     5041 cattttttcc acaataccgt ttttcaccag catcgaataa cgccaagaac gtttaccaaa
     5101 cccaaggtca tttttatcaa ctaatagccc cataccttct gtgaactcac cattaccatc
     5161 aggaataaca gtaatatttt cagattcttg atctgctttc caagcgttca ttacaaaggt
     5221 atcattaact gacatacaga tgatatcatc tacccctaat gccttaaatt cacaagctaa
     5281 ttcattatag cgaggcaaat gtgttgatga acaagtcggt gtaaatgctc cgggtaatga
     5341 aaacacaacc accgttttgt tattaaataa ctcatcggtt gtgacatcaa cccaagcatc
     5401 gttttgacgt gtacgaaatg ttacactggg tactttctga cccgtcatat ctttaaaaga
     5461 catattaatg ttctcctata gaattatata ttataaaaat cttaggcatt atactcacaa
     5521 atatggcata attcagccta tcggaacaat aggaaaaatt taaattggag gaatcaagtg
     5581 aatattcgag atttagaata ccttattgca cttgcagaac ataagcattt ccgtcgtgca
     5641 gcagaatctt gtaatgttag ccaacctact ttaagcgggc aaattcgcaa attagaagac
     5701 gaactaggca ctattctgct tgaaagaacc agtcgcaaag tcctctttac gcaagcaggc
     5761 ttaacattag ttgaacaagc caaggtagtt ttacgtgaag taaaaattct aaaagaaatg
     5821 gcaagcaacc aaggtaaaga gatgtctggc ccgcttcatg ttggtgtaat cccaaccttg
     5881 gggccttaca tttcgccaat tattgtgcct gcattaaaag cagaatttgc agatttagag
     5941 ctgtatattt acgaattaca aaccaaccaa ttattagatc aacttgaatc cggccagctt
     6001 gattgtggca ttatctcatt tgtaaaagag agtgaatctt ttattgaagt gcctatcttt
     6061 aatgaagaaa tgtacttggt agttccagaa caacacgaat gggcagcgct taaaaaattc
     6121 gatatgaatt tattaaaaaa ccgtgagttg ctctttttag acagtggcca ttgtttacgc
     6181 actaatacgc tagactattg tctatccatt ggagcaaaag aaaatccaca ctttaaagct
     6241 accaaccttg aaaccttacg caatatggta gcagcaaatg ttggcctagc attgattcct
     6301 aaattagccg ctaaaccgat taaaggagta cattatattc cacttgaaaa cccgaaaccg
     6361 tttcgagctg tgggaatggt gtatcgacca ggttcaccat tacgcgcccg ctatgagcgt
     6421 ttagctcaag ccattactaa aattatgcaa tccgaggcgt aaaaatggca aacggtatga
     6481 gtgtgcgcgc aattcagaaa gaaaaaacac gcagagcatt agtagaagcc gcctttaatc
     6541 aattaagctc agaaaaaaat ttctctaatc tcagtttgcg tgaagttgcg cgtgaagctg
     6601 gagtcgcacc gacttcgttt taccgccact ttaaagatat ggacgaacta ggcttagcaa
     6661 tggtggacga atccgggcta ctattacgcc aattaatgag gcaagctcga aaacgaattg
     6721 caaatggcgg aagtgttgtc gtggtttcag tggaaacatt cttcgaactg attgccgacc
     6781 gccctaacgt tttccagcta ttactacgcg aaagctccgg tacatcgcaa gcgtttagaa
     6841 ccgcagcatc acgcgaaatt caacattttg tagctgaatt ggcggactat attatgagca
     6901 aagataatca cagtaaccga gaacttgctt atattcaatc ggaaggttta gtcacgctag
     6961 tatttactgc cggttctcat gctttggata tgaacaatca agaacgggaa aaactaaagc
     7021 aacgtgtcat tatgcagcta agaatgctga ttcgtggctc cacctattat gccaataaac
     7081 aagcttcatc aacataaaaa aacagacgcc taagcgtctg tttttattct ttcgtaatat
     7141 atgcagtcga tagctttaac cattctagtg ctaattgatg atgttcagat aacgcattcc
     7201 ataacgcatc ttctgacaag atactataat tgcctttagt atctaactgt tcatcaaaac
     7261 tatcatgtaa ttcacgccat tccgggctag cataaaaccg acttaagggt tccaaaagtg
     7321 cagaagctcg tttccattgc tcaatcccct tttccaattc tggtaatagc tctacccatt
     7381 ggttataaca atcctgcatt ttctgaatat ctgctttttt cattctttac cgcttacacc
     7441 ataaatttta gcctcaatat aacataaaaa tccccttccg aagaagggga tttcaactca
     7501 attaggctaa attaccactg gtatccaacg cctacaccgc caccaaagtc gcctcggcta
     7561 ttcgcattac cttgtaattt caagataagc ttaccgttat cacttgaacg tgaataacct
     7621 actgcaagcg cgttttcacc tttaaatgta ccacctgctg cagccaccat agatttacct
     7681 ggaatgtaaa cctgcggtaa cgctgctgca tttgaaccgg caataccagc acgggcttca
     7741 cggttattac gatggatctt gttatttaag tgattaacag caccttttaa ctgaccaaca
     7801 ttaacagcat cggtatctgc ctcacctgct ttaacattag taatacggtt accaccatta
     7861 tttaagccat ctttcgttaa gcttaccgct tttcctgcag agccattatt aatggttacg
     7921 ccattattgt tcaccaatgt atcaccagtc ttaacgctag taaattcagg ggtatctgaa
     7981 acggctacag ttacatcaga acctttacgt gtcactttga tattattacc tgcattcacg
     8041 ctcactgtac tacctgcttt tactttcgca ggggctttat ccccttcaac cacacctgta
     8101 ccaactgctt tagaatctac gttccagctt acgttgttta cggcatttgc tactgttgtt
     8161 gcattcacaa agtgtgaacc ggtaccgtta ggatcttgaa cattgatttt gccgttatca
     8221 gcaacggtta aatctgtgct gtttacgttc actttcacct caccagcctt atcactggta
     8281 atagttgtac cattaccatt tacgatattg atagaaccat cttcatcagt accaatgtta
     8341 ttaccattca ctttcgcata gttgttcagc tgtttaccgt tgataacatc tgtgcttact
     8401 gggctaatat cgccatctgc tacattagtg atcttagttg gtttaccgtc tttatcaccg
     8461 actttgatgt tatcaccatc attagtgatt ttcggaccat tctcactgaa ctgtacgcta
     8521 ttgaagctca catcttttgc tgtcgcaaaa gtaaattgat taccatcacg tttaacagtt
     8581 aagttatcac ctgcttcaaa cacaacttct tcgcccgttt tcactagctt agtcgcagct
     8641 tggtcaccgg cagccttatt acctgcagat ttaatattaa agcctgcatt attcactgca
     8701 tctgcaacag tttgagcagt cactaacgca tttggctgag ttggtgcttt caccgtacca
     8761 tcttcgttgt tacctaactc actggtattt gcagtgatgt taccttcatt atctacttta
     8821 atagtagtac cgtcagtttt cgcttcaacg ttataagtag ttgaaccatc agcattcgtg
     8881 ctaggtgtga tagttacacc tcggttaccg cccactttcg tagtagctgc attagttgcc
     8941 gcttgtaaat cacctaagtt cactacgttg tttagcttgt cgccttcagc atttttaatc
     9001 gcttctgcac ggtctttacc ctctggggtt gagccatctt cgttacgaac actacttgcc
     9061 acattgccta attgaacagg ggcttcacct gtgaatttag ccgtattgct tggcgttgag
     9121 ctatcagtag gatcttggtt tacaaactca atcgggctat ctactttaat cgtattctta
     9181 ccgtctttgt tttcaaccgt gaccgttgta cctttaccgt taataaaatc aaccgtgtca
     9241 ttatgtacca cagtatcaac cgctttgccg ttaccttgta aattccagcc tgcatttaat
     9301 acatcaccta atgttgctgc attacttaac ttatcagcta agttggttgg attattgtct
     9361 gctggttgaa ccttatcatc tgcagtcaca ggtgtcaggt tagacgcaac attgctaatc
     9421 gctttattac ctgcattgat accatcttta ttgatagtaa ttggattatt tgcaggatct
     9481 gcttcatcag ttggtacagt tacagtgtta gaggttacgc tgctaaactt aacatcatct
     9541 ttagtcgcat aaatgacttt accatcagct tcttgcttca cagttaagtt tttacctgca
     9601 accatttcaa ctgctttacc cggattaatc agttcatcac cacctgaaag cttctctcca
     9661 ccttctacag ccgacgtttt caacgtaaag cctgagttat tgatggcgtt ggtaatatca
     9721 cctgctgtca ctaatttctt agcatcatca gcggttggtt catcaattct accgtcatta
     9781 tttgcatcat ttaatgccgt agtgttagcc ttgatcgcac cggttgtagg atcttttaca
     9841 attgttacat cgtccgtatt cacagaaaga tcgaacacat tcgcgccatc agcatttttt
     9901 gtcgtgttaa ccgtaactga tttatcagtc gacttcactt cttcacgaga tgccatagcg
     9961 aaagtataaa gctgactacc atttactgca tctttactat ctgcatttac gtcaccggca
    10021 gctacatta
//
LOCUS       DANKBV010000080         9924 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.080, whole genome shotgun sequence.
ACCESSION   DANKBV010000080 DANKBV010000000
VERSION     DANKBV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.080"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..163
                     /locus_tag="PWY43_001635"
     CDS             <1..163
                     /locus_tag="PWY43_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HDL2136503.1"
                     /translation="AAYADTIIAMKDGGIKMIGTPKEIITTENLKTVFNLDAEVLECQ
                     GKPLVIHHI"
     gene            complement(340..2907)
                     /gene="glnD"
                     /locus_tag="PWY43_001636"
     CDS             complement(340..2907)
                     /gene="glnD"
                     /locus_tag="PWY43_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDL2136504.1"
                     /translation="MMKNSNELKTLLSAFTEQQRQAFQSENVLSLLAQRSQFYDQLLQ
                     ALWQDFGLAQYEHITLLAVGGYGREEMFPLSDLDILVLTEKPLEEAIQQRLNELFALL
                     WDSKLQLGTSVRTLEECIQIGKAEISVATNMLEGRFLLGNKSLWLRLKEALYQPDFWD
                     IKAFFRAKMAEKNERYARYHNTSYNLEPDLKHSPGGLRDLHLISWIMLRHYGVSSFVD
                     LLNKGLLYPEEYKELIAAQAVLFRMRFALHLQLKRYDNRLRFDRQLQLSEILGYQGEA
                     NQAVELMMRDYFQATKSISQLSQLLLNSFEQAYVNELQKNAQKQPLDQYFYRQEQQLF
                     VVERKIFKTQPTTMLDLFFHLTNDPTLIANADTLRQLRLCLQQQHEPMCFLPEMREKF
                     VQLFAQPKVISRAIVPMHQLGFLSAYLPQWKGIEGLMQFDLFHIYTVDEHTVRVMLNL
                     ENFSEDPSRAFFPLCHQLFATLSEKPLLYLAALFHDIGKGREGEHAQVGALDMREFAE
                     LHGFSQAQINDMVWLVAEHLTMSITAQRRDIHDPQIISEFAKKVQNQTALSSLLCLTV
                     ADICATSESLWNDWKASLFTQLYQFTLQQLAQNLNYQAVAQEHRLQALELMKFVLSAD
                     ERIALNAFWQPCPERYFLHHTPTQLVWHALHYVKTRRLPMVLISNEYARGGTEIFVCC
                     QDQPQLFARIAQLLSQKRISIHDAQIITTENGVVLDSFIVSERDGDDDLTDERCMQIQ
                     QAMMKMLSLTETGIKFTKKPVKHLSFKRKTRLRFLPPSSKQQTEFELFTLDREGLLAQ
                     IGYIFNRLRLNLINAKITTIGERVEDFFVVSNDQGKALSEQEQLELKAILITELDSE"
     gene            3019..3996
                     /locus_tag="PWY43_001637"
     CDS             3019..3996
                     /locus_tag="PWY43_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDL2136505.1"
                     /translation="MTLAVAAIIGGLIILVWSADRFVEGAASTARHFGMTPLLIGIVV
                     IGFGTSAPEMLVSASSALSGSPGIALGNAYGSNITNIALILGLTAIIKPLSVNSDVLK
                     KELPILLAVTLLSAYLVYDANVTQLDAIILLAVFFVYMAWTIITAMKSKNDALSLDVD
                     AELTNSAKMGLGKSILWLIIGLVSLVISSQFLVWGAVEVAKFFGVSDLVIGLTIVAVG
                     TSLPELASSIAAARKGEVDLALGNIIGSNLFNTLAVVGIAGAIEPMQASAEVFSRDVL
                     IMSALTFLLFIFGINLYRRTEGGRINRLEGLLLFAAYVGYNFYLFKTAV"
     gene            complement(4053..5135)
                     /gene="serC"
                     /locus_tag="PWY43_001638"
     CDS             complement(4053..5135)
                     /gene="serC"
                     /locus_tag="PWY43_001638"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDL2136506.1"
                     /translation="MTEIYNFSAGPAMMPKKVLEKAQQELLNWLDQGTSVMEVSHRGK
                     LFMELAAQSEADLRKLYNVPDNYRILFLQGGARGQFAAIPMNLIGKKGKALYLNSGHW
                     SATAAKEARNFAEIDEINILETEPQLKVSRLDFSDIAEQYDYVHYCTNETISGVEIFD
                     IPNVGNTPLVADMSSNILARGIDINKFGVIYAGAQKNLGPAGITIVIIRDDLIGNARK
                     DTPSIWNYATQRDADSMINTPPTFAWYLCSLVFQHLLAEGGLKATEERNLAKAMLLYE
                     YLDSSKFYYNTVAHENRSLMNVTFTTGNDELNAKFVAEATACGLQALKGHKVLGGMRA
                     SIYNAMPIDGVKALIEFMKAFEQCNA"
     gene            5467..6147
                     /locus_tag="PWY43_001639"
     CDS             5467..6147
                     /locus_tag="PWY43_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HDL2136507.1"
                     /translation="MALNNILGLFAQSPLKPLQKHSNKVTECSELLESFFEATFEHDW
                     EKAAEVRGKIVDLERRADALKREIRLKLPRGLFMPVERGDLLELVTQLDKLANYSRDI
                     SGRIIGRKLIIPTEMQPLFKKFLSRSIDASRQVRKVLDEMDQLLETGFRGRELDFVNK
                     MILELDQIEDDTDQYQITLRHTLLELEKNLNPIDVMFLYKCIERISVLADQAQRIGSR
                     IELMLAKS"
     gene            6159..7427
                     /locus_tag="PWY43_001640"
     CDS             6159..7427
                     /locus_tag="PWY43_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDL2136508.1"
                     /translation="MELLNQYGSILVIITAIVGFMMAFGIGANDVSNAMGTSVGSGTI
                     TIKQAILIAMVFEFAGAYLAGGEVSDTIKSGIIDPAQFANSPDILVLGMMASLCAAGM
                     WLIIATKMGWPVSATHTIIGAVIGFALITIGSDAIQWDALGGIVGSWFVTPILAGIVA
                     YLIFINSQKLIFNRSNPFKQAKKYGPMYMGITIFILVLVTVSKGLKHVGLHLDTAETL
                     GISFALALLAFIIGYFYFRSKSFAMKARAEGKGFAGVEKIFSILMLITACAMAFAHGS
                     NDVANAIGPLAAVESIVRSGGMIEGKTTLAPWILPLGALGMMIGLAIMGKNVMATVGT
                     GITDLTPSRGFAAQFACAVTVVIASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVI
                     RNIVISWVITLPVGAVLSIIIYYILSAIFY"
     gene            7482..8093
                     /locus_tag="PWY43_001641"
     CDS             7482..8093
                     /locus_tag="PWY43_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDL2136509.1"
                     /translation="MKKLSSALLSCFLLGLSSPALTADTYITENLSTFMRKGAGDQFR
                     ISGAIQAGEKVTVLDRKERYSLIRDSRNREGWVLNSDLSDTASPKELIPQLKQQVQDL
                     TNRLSKIDTEWQQRTAEMQRRTQDSVQKSSDLVEENAQLKRELEILKNKNRDLETMHD
                     SEKREIVIQWFIYGGAVLGAGLLLGLLVPFLIPRRRRNNSGWA"
     gene            complement(8147..9445)
                     /gene="aroA"
                     /locus_tag="PWY43_001642"
     CDS             complement(8147..9445)
                     /gene="aroA"
                     /locus_tag="PWY43_001642"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2136510.1"
                     /translation="MEKLTLTPISRVEGEINLPGSKSLSNRALLLAALATGTTQVTNL
                     LDSDDIRHMLNALKALGVKYELSDDKTVCVVEGIGGAFKVQNGLSLFLGNAGTAMRPL
                     AAALCLKGAETAQIILTGEPRMKERPIKHLVDALRQVGAEVQYLENEGYPPLAISNSG
                     LQGGKVQIDGSISSQFLTALLMSAPLAESDMEIEIIGDLVSKPYIDITLSMMNDFGIT
                     VENRDYKTFLVKGKQGYVAPQGNYLVEGDASSASYFLASGAIKGKVKVTGIGKKSIQG
                     DRLFADVLEKMGAKITWGEDFIQAEQSPLKGVDMDMNHIPDAAMTIATTALFAEGETV
                     IRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLALENFQHAEIETYNDHR
                     MAMCFSLIALSNTEVTILDPNCTAKTFPTYFRELEKLSVR"
     gene            complement(9491..>9924)
                     /locus_tag="PWY43_001643"
     CDS             complement(9491..>9924)
                     /locus_tag="PWY43_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2136511.1"
                     /translation="RSLSKAYGLAGLRIGYAVSNPEIADLLNRVRQPFNCNSLALASA
                     IAVMNDDEFVKKVAENNRLEMARYEAFCQANGLEYIPSKGNFITIDFKRPAAPIYEAL
                     LREGVIVRSIAGYGMPNHLRISIGLPQENERFFTALLKVLA"
BASE COUNT         2835 a         2011 c         1938 g         3140 t
ORIGIN      
        1 tgctgcttac gcagatacta ttattgcaat gaaagatggt ggaattaaga tgatcggcac
       61 cccaaaagag attatcacca ctgaaaattt aaaaacagtg tttaatcttg atgccgaagt
      121 gttagaatgc caaggcaaac ctttagttat tcatcatatt taacgtttga agatcatggt
      181 aattaataaa aattgcgtct taatttatta atctaaacga tagaaactta tatttagttt
      241 ctaatttctt gaattctcaa cctctcttaa ctcgtgattt aatttaccta aaacgttgaa
      301 aaatttaccg cttgcagtct tcactccaag cggttgtttt cattcactat ctagttcggt
      361 aattagaatg gcttttagtt ctaactgttc ttgttccgat aaagcttttc cttgatcatt
      421 actgaccaca aagaaatcct ctaccctttc accaatagtc gtaattttcg cattaattaa
      481 atttaatcgg agccggttaa aaatatagcc tatttgtgcc aacaaccctt ctctatccaa
      541 ggtaaaaagt tcaaattcgg tttgttgctt tgatgacgga ggaagaaaac gcagccgagt
      601 tttacgctta aatgacaaat gtttcaccgg ttttttggta aattttatgc ctgtttcagt
      661 taagcttaac attttcatca tcgcctgctg aatttgcata catctctcat cggttaaatc
      721 atcatcacca tcacgctcac ttacaataaa gctgtctaac actacaccat tttcagtagt
      781 aataatttgt gcatcgtgaa tgctaatgcg tttttggctg agtaattggg cgattcgagc
      841 aaacagttga ggttgatctt ggcaacatac aaaaatttct gtaccaccac gggcatattc
      901 attactgatt aaaaccatcg gcaaccggcg agtttttaca taatgcagag cgtgccatac
      961 caactgagtc ggtgtatggt gaagaaaata acgctccggg caaggttgcc aaaatgcgtt
     1021 taatgctatt cgctcatcag ctgaaagcac gaacttcatc agttctaatg cttgcaagcg
     1081 atgctcttgt gccactgctt gataattcaa attttgagcc agttgctgta gcgtaaattg
     1141 atataattgg gtaaacagcg aggctttcca atcattccag aggctctcac tggttgcaca
     1201 aatatctgca accgttaaac aaagtagtga agataaagcg gtctgatttt gcactttttt
     1261 tgcaaattcg ctgatgattt gcggatcgtg gatatctcgt ctttgtgcgg tgatcgacat
     1321 tgtcaaatgc tctgccacca gccaaaccat atcattgatt tgtgcttgag aaaaaccgtg
     1381 tagttccgca aattctcgca tatccagcgc accaacttga gcgtgttcgc cctctctacc
     1441 cttgccaata tcgtggaaaa gagccgcaag atagagcaac ggtttctcag aaagcgttgc
     1501 aaatagctga tgacatagtg ggaaaaacgc ccgacttgga tcttcgctaa aattttctaa
     1561 atttaacatg actctgaccg tatgctcatc gactgtataa atatggaata ggtcgaattg
     1621 cattagccct tcgatcccct tccattgcgg tagataagca cttaaaaaac ccagttggtg
     1681 cattggaact attgctcggc ttatgacttt cggttgagca aaaagctgta cgaatttctc
     1741 tcgcatttca ggcaaaaagc acataggctc gtgttgttgc tgtaaacaca gtctcaactg
     1801 acgtaaggtg tcggcattcg ctattaacgt agggtcatta gtaagatgaa aaaagagatc
     1861 caacattgtt gtgggttggg ttttaaatat tttgcgttcg accacaaaca attgctgttc
     1921 ttgccgataa aaatactgat caagcggttg tttttgtgca tttttttgca actcgtttac
     1981 ataggcttgt tcaaaactgt ttaacaataa ttggctcagt tgcgaaatag attttgtggc
     2041 ttgaaaataa tctcgcatca ttaattccac cgcttgattt gcttcgcctt ggtagcctaa
     2101 tatttcactc agttgtaatt gtctgtcaaa ccgaaggcga ttatcgtaac gtttcagttg
     2161 caaatgaagt gcaaaacgca tccgaaaaag taccgcttgt gctgcgatca gctccttata
     2221 ttcttccggg taaagtagcc ctttattgag gagatcgaca aaagaagata caccataatg
     2281 ccgtagcata atccaagaga ttaaatgcag atctcgcaat cctcccgggc tatgtttgag
     2341 atctggctca aggttatagc tagtattatg ataacgtgca taacgctcat ttttttccgc
     2401 cattttcgct ctaaaaaacg ctttaatatc ccagaaatcc ggctgataaa gtgcttcttt
     2461 taatcttagc caaagcgatt tattacccaa taaaaatcgc ccttccaaca tattagtggc
     2521 aaccgaaatt tctgctttcc caatctgaat acattcttcg agcgtacgca cactggtgcc
     2581 aagttgcagt ttgctgtccc aaagcaaggc aaatagttcg tttaaacgct gctgaatagc
     2641 ctcttctagc ggtttttcag ttaacactaa gatatccaga tccgaaagcg gaaacatctc
     2701 ctcacgtccg taccccccta ccgctaatag ggtaatatgt tcatattgag cgagtccaaa
     2761 atcttgccaa agtgcttgca gtagttggtc ataaaattgg ctacgttgtg caagtaagct
     2821 gagtacattt tcggattgaa atgcttgccg ctgttgctcc gtaaatgctg ataaaagcgt
     2881 ttttaattcg ttgctgtttt tcatcataat ctcttcctaa actggcaaaa taataggtca
     2941 tttattttcg tttgcctata tttattttgg ctaaaatgct tataatgcct ttatgttttt
     3001 catctaaaag gatagtttat gacacttgct gttgccgcta ttatcggtgg cttaattata
     3061 ttagtttgga gtgcggatcg ttttgtagaa ggggcagcct ctacggctcg ccattttggt
     3121 atgacaccat tgttaatcgg tattgtggtc attggttttg gtacatctgc accggagatg
     3181 ctcgtatccg catcttctgc attaagcgga tcgccgggta tcgctctcgg taatgcctac
     3241 ggttctaaca ttaccaatat tgcgctgatt ttaggtttaa ccgcaataat taaaccacta
     3301 tcagttaatt ccgatgtgtt aaaaaaagag ttgccgattt tactggctgt caccctgctt
     3361 tctgcttatt tggtttatga tgcaaacgtc actcaacttg atgcgattat tttacttgct
     3421 gtgttttttg tatatatggc gtggacaatt attaccgcaa tgaaaagtaa aaatgatgca
     3481 ttaagcttag atgttgatgc agaattaacc aatagtgcta agatgggatt aggaaaatcc
     3541 attctttggc taattattgg tttagtatcg ctagtaatta gttcccaatt cttagtctgg
     3601 ggagcagttg aagtggctaa attttttggt gtgagtgatt tggtaatcgg gcttactatt
     3661 gtggcggtag gcacctcact acctgaatta gcctcttcta ttgcagctgc tcgtaaagga
     3721 gaagtggatt tagctttagg taacattatc ggttctaact tatttaatac gttagccgtg
     3781 gtggggattg ccggtgctat agaaccaatg caagcttcag cggaagtgtt ttctcgagat
     3841 gttttgataa tgtctgccct taccttctta ctattcattt tcggaatcaa cctctatcgt
     3901 cggacagaag gtggcagaat taaccggtta gagggcttat tactctttgc cgcttatgta
     3961 ggctataatt tctatttgtt taaaactgcg gtctagtacc aaattcaaag agccacaaac
     4021 gtggctcttt aatttatatg tttaagcttg aattaagcat tacattgttc aaatgccttc
     4081 ataaattcaa tgagtgcttt caccccatca atcggcattg cgttataaat tgaggcacgc
     4141 attccaccta atactttatg gcctttcaat gcctgtaaac cacaagcggt cgcttctgcc
     4201 acaaattttg catttagctc atcattacca gtggtaaaag tcacattcat caaggaacga
     4261 ttttcgtgtg ctaccgtatt gtaatagaat ttagagctat ccaaatattc ataaagcaac
     4321 atagctttag caagattacg ttcttcagta gctttaagtc caccctctgc cagtaaatgt
     4381 tggaacacta aagaacataa ataccaagca aaagttggcg gggtattaat catcgaatca
     4441 gcatctcgct gagttgcata attccaaata gatggggtat ctttgcgtgc attgccaatt
     4501 aaatcatcac gaataatcac aatcgtaatg ccagcaggcc ctagattttt ttgcgctccg
     4561 gcataaatta ctccaaattt gttgatatca atccctcggg ctaagatatt agacgacata
     4621 tctgccacta acggggtatt tccgacattc ggaatatcga aaatttctac cccactgatg
     4681 gtttcattgg tgcaataatg gacataatca tattgctctg caatatcgct gaaatctaaa
     4741 cgactcactt taagttgcgg ttcagtctct aaaatattga tttcatcaat ttccgcaaag
     4801 ttacgtgctt cttttgctgc tgttgccgac caatgccctg aattaaggta aagcgctttg
     4861 ccttttttac cgattaaatt cattggaatg gctgcaaatt gcccccttgc accaccttga
     4921 agaaatagaa tacgataatt gtccggcaca ttatacagct tgcgtaaatc tgcttcagat
     4981 tgtgccgcca gctccataaa tagctttcca cggtggctga cttccatcac tgatgtgcct
     5041 tggtctagcc aatttaataa ttcttgttgc gctttttcga gtactttttt cggcatcatt
     5101 gccgggccgg cactgaagtt atatatctct gtcatttttc acctcgtata gtaataatat
     5161 tgattctatt ctatctgaaa aaaagcgtaa tggtagaata aaaacagaaa aattatttta
     5221 gcaaacttgc ataaaatact ctttttaaaa gcataatact ctttcgtaac atcacccaaa
     5281 taaggaataa gcgatataaa aaaacaagca taaatttctt ccattttttg caaaaaaatt
     5341 attaaaacaa accgcttgtc ttaaaaacat aatttaattt cgtgatcttt actaatacat
     5401 caagtaaaat acgccggact ttacagtaag aagtcattgt caatattatc actatggaga
     5461 tattttatgg cattgaataa cattttaggt ttattcgccc agtccccact taaaccgtta
     5521 caaaaacact caaataaagt gactgaatgt agcgagttat tagaatcttt ttttgaagcg
     5581 acttttgaac atgattggga aaaagcagct gaagtgcgcg gtaaaattgt tgatttagaa
     5641 cgccgcgcag atgcccttaa acgtgaaatc cgcttaaaac tgcctcgtgg tttatttatg
     5701 ccagttgaac gcggtgattt attagaacta gtcacacaat tagataagtt agcaaactat
     5761 tcaagagata tttccggtcg cattattggt cgtaaattaa tcattcctac cgaaatgcaa
     5821 ccacttttca aaaaattcct ttctcgtagt attgatgcta gccgtcaggt acgcaaagtt
     5881 ttggatgaaa tggatcagtt attagaaact ggttttagag ggcgagagct tgatttcgtc
     5941 aataaaatga ttcttgaact tgaccaaatt gaagatgata ccgaccaata tcaaattaca
     6001 ttacgtcata cacttcttga gttagaaaaa aacctgaacc ctattgatgt gatgttttta
     6061 tacaaatgta ttgaaagaat cagcgttcta gctgaccaag cccagcgaat tggttctcgt
     6121 attgaactta tgctggcgaa gtcataggag aaaattaaat ggaattactc aatcaatatg
     6181 gctcaatact ggtgattatt actgctattg tagggtttat gatggctttc ggtattggtg
     6241 ctaatgatgt ttcaaatgcc atgggaacat ccgtcggctc tggaacaatt acgattaaac
     6301 aagctatttt aattgctatg gtatttgaat ttgctggtgc ttatctggca ggcggtgaag
     6361 tctcagatac aattaaaagt ggcattattg atccggcaca attcgccaat agccctgata
     6421 tcctggtact tggaatgatg gcatctctct gtgctgccgg aatgtggcta attattgcaa
     6481 ctaaaatggg ttggccggtt tcagcaactc acacaatcat tggtgccgtt attggttttg
     6541 cattaatcac tattggttca gatgcaattc aatgggatgc attaggtgga attgttggga
     6601 gttggtttgt gacccctatt cttgcgggga tcgtagcata tttgatcttt attaactcac
     6661 aaaaactgat ctttaaccgc tcaaacccat tcaaacaagc gaaaaagtat ggcccgatgt
     6721 acatgggaat tactattttt attttagttc ttgtaaccgt gtcaaaaggc ttaaagcacg
     6781 taggattaca tctagataca gctgaaacat tgggaatttc attcgcgctt gccttacttg
     6841 cttttatcat tggatatttt tatttccgca gtaaaagctt tgcaatgaaa gctcgtgcag
     6901 aagggaaagg cttcgctggc gttgagaaaa tttttagtat tttaatgctt attacggcct
     6961 gtgctatggc ttttgctcat ggctctaatg atgttgctaa tgctattggg cctctagctg
     7021 ctgttgaatc tattgtaaga agtggtggaa tgattgaagg taaaacgact ttagcccctt
     7081 ggattctccc actaggcgcc ttaggtatga tgattggttt agctattatg ggcaagaatg
     7141 taatggctac agtaggtact ggtattaccg accttactcc aagccgtggc tttgctgctc
     7201 aatttgcctg cgccgtcacg gttgtcattg catcaggtac tggtttacca atatcaacaa
     7261 cccaaacgct tgtaggagca gtattaggtg tgggattcgc ccgtggtatt gctgcactta
     7321 acttaggcgt tattcgtaat attgttatat catgggtaat taccttgcct gtaggtgctg
     7381 tgctttcaat tattatctat tatattttaa gcgctatttt ctattaattt ttcaaaaaga
     7441 atacaggcaa ctgtattctt cctagcttta gaggaatata tatgaagaaa ttatcatcag
     7501 ctcttctgtc ttgtttttta ctcggcctat ctagccctgc tttaacggca gacacttaca
     7561 ttactgaaaa tctgagtaca tttatgcgca aaggtgctgg tgatcaattc cgcatttcag
     7621 gcgctattca agcaggtgaa aaagtcacag tattagatcg taaagagcgc tacagtctga
     7681 ttcgagatag tcgtaaccgt gaaggttggg tacttaattc agacttaagc gatactgcta
     7741 gtccaaaaga gctgattcct caattaaaac aacaggttca agatttaacc aatcgcctga
     7801 gcaaaatcga taccgaatgg caacaacgta ccgcagaaat gcaacgtaga acgcaagatt
     7861 cagtgcagaa aagcagtgat ttggttgagg aaaatgcaca actaaaacgt gaactggaaa
     7921 tcctgaaaaa caaaaatcgt gatttagaaa caatgcacga ttccgagaaa cgtgaaatcg
     7981 tgattcagtg gttcatctat ggtggtgctg tgttaggcgc aggtttacta cttgggttac
     8041 tcgtgccttt cctgatccct cgccgcagac gtaacaacag cggttgggct tagtctgcca
     8101 caataaaacg taaaaaggat tcagttttct gaatcctttt ttacttttat ctgaccgata
     8161 atttttccaa ctccctaaag taagtcggga acgttttagc ggtacaattt ggatctaaga
     8221 tcgtcacttc tgtattcgat aacgcaatta atgaaaaaca cattgccata cggtgatcgt
     8281 tataggtttc aatttcagcg tgctggaagt tttctaacgc aagcggttga atccgaataa
     8341 aatcttcccc ttcttctacc tctgccccga ctttacgcaa ttcggttgcc attgctgtca
     8401 agcggtcggt ttcttttacc cgccagttat aaatattgcg gataactgtt tctccttcgg
     8461 caaataaagc ggttgttgca atcgtcattg ccgcatcagg aatatgattc atatccatat
     8521 ctacgccttt tagcggggat tgctcggctt gaataaaatc ctctccccaa gtgatttttg
     8581 cccccatttt ttccaacaca tcggcaaaca agcggtcgcc ttggatcgat tttttaccaa
     8641 tgcccgttac ttttaccttg cctttaatcg caccggaggc taagaaataa gaggcagaag
     8701 aggcatctcc ctccaccaaa taattacctt gtggagcaac atagccttgt ttacctttaa
     8761 ctaaaaaggt tttgtaatct cgattttcaa ccgtaatacc aaaatcgttc atcatcgaaa
     8821 gggtaatatc aatataaggt tttgatacca gatcaccgat aatctcaatt tccatatcgc
     8881 tttccgctaa tggggcagac atcagcaatg cggttagaaa ttggctggaa atcgagccgt
     8941 caatttgcac ttttccacct tgcaaaccgc tattgctaat tgctaacggc ggatagcctt
     9001 cattttctaa atactgtacc tctgccccta cttggcgtaa agcatcgact aaatgtttaa
     9061 tcgggcgttc tttcattctt ggctcaccgg taagaatgat ttgagctgtt tccgcccctt
     9121 ttaaacacaa tgctgctgca agtggtcgca ttgccgtgcc tgcattgccg agaaacagtg
     9181 ataagccgtt ttgaacctta aaagctccac caatcccttc aactacacag acggttttat
     9241 cgtccgatag ctcatatttc acgcctaacg cttttaaggc attgagcata tgtcgaatat
     9301 catcactatc taataaattg gtcacttgag tcgtaccggt ggctaaggcg gctaataata
     9361 aggctcggtt agacaggctt ttagaaccag gtaaattgat ctcgccttct actcgggaaa
     9421 tcggggttaa agttagtttt tccatttttc tctttaaatt tgtaaaattt tcataaaagg
     9481 taaccgcttg ttaagctaac actttcaata aggcagtaaa aaatctctcg ttttcttgcg
     9541 gtaaaccgat actaatgcgt aaatgattcg gcattccata acctgctatc gagcgaacaa
     9601 tcacgccttc acgcagtaat gcctcataaa tcggagcggc aggacgtttg aaatcaatag
     9661 taataaaatt gcccttagaa gggatatatt ccaagccgtt tgcttggcag aaagcctcat
     9721 agcgagccat ttccaagcgg ttattttctg ccactttttt gacaaattca tcatcattca
     9781 ttaccgcaat cgctgaagct aaggcaagac tattgcagtt aaacggctgg cgaacacgat
     9841 ttaataaatc cgcaatttca gggtttgaaa ccgcataacc aatccgaagc ccggctaagc
     9901 cgtaagcctt agagagtgaa cgtg
//
LOCUS       DANKBV010000081         9907 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.081, whole genome shotgun sequence.
ACCESSION   DANKBV010000081 DANKBV010000000
VERSION     DANKBV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.081"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            27..761
                     /locus_tag="PWY43_001644"
     CDS             27..761
                     /locus_tag="PWY43_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2136512.1"
                     /translation="MARSSYFARLAEWTLLEHLLVKQPENISVCSFGQSFGTSGRLDA
                     EYYQPKFASLFAQLANFKTMKLGEIVNLQKSVEPGSEAYQTEGIPFVRVSDLSKFGIS
                     QTDKYLHPKDFGNVVRPKKDSILLTKDGTVGIAYRVPQDLNVITSGAIVHLELKTDEV
                     LPDYLALALNSPAVQLQAERDAGGSIIQHWKPSEILDVVIPVLPKNIQQTISDKVQQS
                     FALRVESEVLLEKAKILVEQEIENMR"
     gene            764..1398
                     /locus_tag="PWY43_001645"
                     /pseudo
     CDS             764..1398
                     /locus_tag="PWY43_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1615..2364)
                     /locus_tag="PWY43_001646"
     CDS             complement(1615..2364)
                     /locus_tag="PWY43_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2136513.1"
                     /translation="MIKIENLTLAHGIQHLSCYLPKGKLIGIMGANGAGKSSLLKAIA
                     GILRPTSGEIWLENCKLHALSAVERSRQFAYLAQQLNVAWQLSVYDVIALGLPAPLSP
                     EQERQKVRSVAEQLAISHLLDNTYQCLSGGEQTRVQLARCLMKQAPILLADEPIAALD
                     PYYQIDIMQQLKALTPTQTCVVVIHHLPLAYRFCDEIILLKNGNLLANGNTQAVLTAE
                     NIATAFGVKAEMDWEKREMWGIEKLEMNFQF"
     gene            complement(2361..3329)
                     /locus_tag="PWY43_001647"
     CDS             complement(2361..3329)
                     /locus_tag="PWY43_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2136514.1"
                     /translation="MKKLNIVLGSLLLLIVSSAAFYQLGDFALLKNAEGVLSDLRWLV
                     LGEIRLPRIGLAVLTGASLALAGNAMQGVFQNPLASPGLLGSSSGATAVSVFLLYYFS
                     APMGWLLFGGVAGALGAFLLVWLISFRHGTTMMILSGVAVNVLLGAAVTLLLSNAESP
                     WALAELYRWLQGSLVWATAEAVCWAFPLILLGIVCLYRERRYLDLLTFGEETAATMGI
                     NLQRSFFTVSIGVALLVGATIPQTGTIGFIGLIAPHLARIWLKKPPSQLYLTSALIGA
                     LLLLIADLAVQYVPFFARIYIGTLTAILGAPFLIWILFTQQRRLAQ"
     gene            3510..3847
                     /locus_tag="PWY43_001648"
                     /pseudo
     CDS             3510..3847
                     /locus_tag="PWY43_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3828..4151
                     /locus_tag="PWY43_001649"
     CDS             3828..4151
                     /locus_tag="PWY43_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL2136515.1"
                     /translation="MNELYDDPIPQATREKYGITEFDAAEYLTNDELIALYLAETLKD
                     GTDEEFIQALNTVARAKGMNELAKKAGIGRESLYQSLSSSKPRFETIRKIISALNLEL
                     SVVKA"
     gene            complement(4141..6027)
                     /locus_tag="PWY43_001650"
     CDS             complement(4141..6027)
                     /locus_tag="PWY43_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL2136516.1"
                     /translation="MTQLDSVTVYSAYATPINQDKTASSVTVLTEKDFAARNATYVSD
                     VLKTVPGVAIGQQGGRGTLTSLFLRGAESRHTAVVIDGVKVNPINIGNFDFGGLPISN
                     IERIEVLRGEQSALWGSSAMGGVVYITTKSGLYKEKPFNAEVDLGLGSNNTRDASATL
                     SGFHNGFYYALHGDSHRTKGISALSKNHFSYTTETGSEVKTGGASERDGFHRDNGSLR
                     LGYDLGNKGVEVLAAQSSQTVHIDGYNSDVSGEYSRTRNQTFKLGGYWGNEQELLKHQ
                     ANISQFNSKATHFGSNARYSNEKQLNANYQLDVNFDREGEVTQAVSLLTDYAKTRYTS
                     DKYLREKTLSEKSAALEYRLFTEQDHSFSISGRYTDNSQFKNSITGRISGAYRLSPNL
                     CSDRLLLELAEPQQIRAMSPYSQKPLMMLDKLNTDKPTVEPELTALLPYADSTILLNE
                     TFYPQLTARLKQLGFKLVALNDSPQTPEQLFTLILQLGELTQNQAKAEKLVERLRLQK
                     IPLKQPLAETLILSETGMIEPHFPQYQTLLHLLGLSPLKSDLTPQNFSLEKLLLAQPK
                     QLLFLTDNQSYNNQAELLKHPALQKIWQKMSQNPPLVLPMKYTYCFDHGVWQGIQLMH
                     KLTP"
     gene            6057..6857
                     /gene="metF"
                     /locus_tag="PWY43_001651"
     CDS             6057..6857
                     /gene="metF"
                     /locus_tag="PWY43_001651"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDL2136517.1"
                     /translation="MQQKWRMLWDSIDRLSPLKPKFVSVTYGANSGERDRTHSIVKSI
                     QEKTGIISAPHLTGIDATPEELKVIAKDYWDSGIRRIVALRGDIPKGYDKALFYASDL
                     VALLKSVADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGANHVITQFFFDVESYLR
                     FRDRCVSIGIDCEIVPGILPVTNFKQLQKMAAVTNVKIPTWMGKMYEGLDDDQTTRNL
                     VAASIAMDMVKVLSREGVNDFHFYTLNRSELTYAMCHTLGVRPDLTTA"
     gene            complement(6906..8066)
                     /locus_tag="PWY43_001652"
     CDS             complement(6906..8066)
                     /locus_tag="PWY43_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136518.1"
                     /translation="MKKSILLILSLFSASLFADVQLSPLSLPQIGKVSQFDLSEQGEL
                     VVINQQKQLWRVTQQAQKLADGVSTEIEPKAQYQRIALADQNGHFMLWTADKIYTSNI
                     PLAPKATMTALNFATIAVTKQNGQFKLARIETQGNQATITATAETEVLPDAHPLQIDF
                     DAETPNQGHIAVLAKPDDFTYQHGVLGDDVEAGEIQFLERHTLNPLAEKLTQQGRVFE
                     ANRWATFTENGKTKIVSVISGDGNGASTVVIGVKDGKLSIEAESEPLSTNRWQSPFIF
                     NRKLYAIQMPHLKGNLVEYSLKGNQLAERFITSGLSNHRYGDHETNLVATTETFALIP
                     QMGYQQVVVLDKTGKITELPTALPAGIKQTKADSNKAYLLLKNGEIWVAEEK"
     gene            complement(8126..9142)
                     /gene="galE"
                     /locus_tag="PWY43_001653"
     CDS             complement(8126..9142)
                     /gene="galE"
                     /locus_tag="PWY43_001653"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL2136519.1"
                     /translation="MAILVTGGAGYIGSHTLVELLNENREIVVLDNLSNSSEVSLERV
                     KQITGKSVKFYQGDILDRDILRKIFAENQIESVIHFAGLKAVGESVREPLRYYQNNVT
                     GSIVLVEEMLKANVNTIVFSSSATVYGDPQIIPIVESCPVGGTTNPYGTSKYMVERIL
                     EDTVKAFPQLSAVVLRYFNPVGAHESGLIGEDPNGIPNNLMPFISQVAVGKLPQLSVF
                     GGDYNTHDGTGVRDYIHVVDLALGHLKALDKHQNDAGFHIYNLGTGTGYSVLDMVKAF
                     EAANGITIPYKVVDRRPGDIAVCYSAPQKALEQLGWKTERGLEQMMKDTWNWQKNNPN
                     GYKG"
     gene            complement(9230..9391)
                     /locus_tag="PWY43_001654"
     CDS             complement(9230..9391)
                     /locus_tag="PWY43_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HDL2136520.1"
                     /translation="MNETLLNNLVCPVSNEKLEWDKENNRLINPKLNIAYPIKNGIPE
                     LLPEAGVAL"
     gene            complement(9369..>9907)
                     /gene="lpxK"
                     /locus_tag="PWY43_001655"
     CDS             complement(9369..>9907)
                     /gene="lpxK"
                     /locus_tag="PWY43_001655"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDL2136521.1"
                     /translation="MEWVVVDGVRRFGNGFVLPAGGLRELPSRLNSVQAVICNGGTPQ
                     ANEHLMTLEPSDAINLVTNQRKPLSEFTQPCVAIAGIGHPPRFFTMLEKLGIPLAKTQ
                     GFADHQAFDKAQLAQFADSQTPLLMTEKDAVKCHDFAQPNWWYVPVSATFSEKSTACL
                     LAPILQKLGKEDERNTTQ"
BASE COUNT         2795 a         2076 c         2197 g         2839 t
ORIGIN      
        1 tagatcctgc taagccttat ggtttattgg cgagatcttc ttattttgca cgtttagcag
       61 aatggacttt attagaacat ttacttgtca aacagcctga aaatatttcg gtatgttcat
      121 tcggtcaatc attcggtaca agcggtcgtt tagatgcaga atattaccaa cctaaatttg
      181 caagcttatt tgcacaatta gccaatttca aaacgatgaa attaggtgag attgttaatt
      241 tacaaaaatc ggtagaaccg ggctcggaag cttatcaaac agagggaata ccctttgtgc
      301 gtgtatcgga tttatctaaa ttcggcatca gccaaacaga taaatactta catccgaaag
      361 attttggcaa tgttgtaaga cctaaaaaag acagtatatt actgactaaa gacggcacag
      421 tcggcattgc ttaccgtgtt ccgcaagatt tgaatgtgat tacatcaggg gcaattgtgc
      481 atctagagct taaaactgac gaagttttac ccgattattt ggcgttagca ttgaattctc
      541 ctgctgtaca attacaagcg gaaagagatg cgggaggctc tattatccaa cattggaaac
      601 cgtctgaaat tttagatgta gtgattccgg tattacctaa aaatatccag caaaccatta
      661 gcgacaaagt acaacaaagt tttgcattga gagtagaaag tgaagtttta ctggaaaaag
      721 ctaaaatact tgtagaacaa gaaatagaaa atatgaggta ataatgccta atcataatca
      781 agatttgaaa aaagagtggt ttaataaagc aaggatagat taccactctc ttttttaatg
      841 ttatggctca gttgtaatag ctggtataac ttccattatt ccttaggtaa ggatagaaca
      901 catattgata gaataaaatc ggataccagt aatcaaaaca aactttataa agagtttgaa
      961 agaatattca cctctggaaa aataaaagaa aaaactaatt tatggaataa tattgagcaa
     1021 cttcattttg cattagttca ggcagagtta aaatattcag gtagcaatat tccaagtgaa
     1081 tattctaaat ttaacttgga aaatgtgctt attgattttc ctaacaagac aaattctgta
     1141 gcatatcaga atctagttat tcacaacgca aaaacgcgtg caggaaaatt aaaaacacaa
     1201 tatgcaaatg ctcacgattt aggcaatttg gttttagtag aggatatcca aaaaatattt
     1261 tccgggttat tagaaataat ttatcaagtt cgttgccatt tggtacatgg tagcttaagt
     1321 ccaacacctg aaaaccacga agttgttaaa tattgctatt tgattttgtg ggattgttta
     1381 aaaggatttt gtgattagtt attgtcaata aagctacaag cggtcaattc tcccaaaact
     1441 ttgcaaattt ccccgcaaaa ctgaccgctt gttctcctct atccattcac ctcaaccaac
     1501 ttttctctct acccctactc aaaatcggtt aaacaatctg ttgaggtaaa tgttgtgtcg
     1561 gaatgggatt taatcttaat gaaactgaaa aaatcttctg ccaagcaaat ttccctaaaa
     1621 ttggaagttc atttccaatt tttcaatccc ccacatttcc cttttctccc aatccatttc
     1681 agctttcacc ccaaaagccg ttgcaatatt ttcggcagtt aagaccgctt gtgtgttgcc
     1741 gttggcgagt aaattaccgt ttttaagcag aatgatttca tcgcaaaaac ggtaggcaag
     1801 gggcagatga tgaatcacca ccacgcaggt ctgggttggg gtgagggctt tgagctgctg
     1861 cataatgtcg atttggtagt aggggtcgag ggcggcaatc ggttcatcgg caagcaagat
     1921 cggggcttgt ttcatcaaac aacgggcgag ttgcactcgg gtctgttcac cgccggaaag
     1981 gcattggtaa gtgttatcca gcaggtgaga aatcgccaac tgttcggcaa ccgaacgcac
     2041 cttttgacgc tcttgttcgg gcgaaagcgg tgcgggcaag ccgagtgcta tcacatcata
     2101 taccgaaagt tgccacgcca cgttgagctg ttgggcaagg taggcaaatt gtcggctccg
     2161 ttcaacggcg gaaagggcgt gcagtttgca attttccagc caaatttcac cgcttgtcgg
     2221 gcgtagaatg ccggcaatcg ctttgagcaa cgaggatttc cccgccccgt ttgcccccat
     2281 aatgccgatc aatttgcctt ttggcagata gcaagagagg tgttgaatac cgtgagcaag
     2341 ggtgaggttt tcaatcttga tcattgtgcc aatctccgtt gctgggtgaa taaaatccaa
     2401 atcaaaaacg gtgcgcccaa aatcgccgtg agcgtgccga tgtaaatgcg ggcaaaaaac
     2461 ggcacatatt gcaccgctag atctgcaatc agcagtaaca acgccccgat taaagcggag
     2521 gtgaggtaaa gctgagacgg tggttttttc agccaaatac gggcaagatg cggagcaatt
     2581 aagccgataa aaccgatagt gccggtttgt ggaatagttg ctccgaccaa cagcgccacg
     2641 ccaatgctaa cggtaaaaaa actgcgttgc aggttgatgc ccattgttgc agcggtctct
     2701 tcgccgaagg ttagcaggtc gagataacgc cgttcccgat agaggcagac aatgccgagc
     2761 aagatcaagg ggaacgccca acatactgcc tctgcggttg cccagactaa tgagccttgt
     2821 aaccagcggt agagttccgc caacgcccac gggctttcgg cattggaaag tagtaaagtc
     2881 accgctgccc cgagcaacac gtttaccgcc acgccactta aaatcatcat tgtagtgccg
     2941 tggcgaaagc taatcagcca caccagcaaa aacgccccca acgcacctgc tacaccgcca
     3001 aacagtagcc agcccatcgg ggctgaaaaa tagtagagca gaaacacgct caccgccgtt
     3061 gcccccgaac tgctcccaag caaaccgggg ctggcaagcg gattttgaaa cacgccctgc
     3121 atcgcattac cggcaagcgc aaggctggcg cccgtcagca ccgccaagcc gatacggggc
     3181 agtcgaattt cgcccagcac cagccagcgc agatcgctta gcacgccttc ggcatttttt
     3241 agcaaggcaa aatcgcccag ttggtaaaag gctgcgctgc taacaatcag caacaacaag
     3301 cttccaagca caatgtttaa tttcttcatt cttctttctc tcatcaaacg tgcagattat
     3361 accatagcgc caaagtcaag ctgaaaacgc tatttttgcg atcgccatcg aaaaaatagc
     3421 gtttgccgaa tgtaacccac gacaggcttt gctatattcc ttcaatcagc ctaagttttg
     3481 tatttaaaac attacagatt gggggtagaa tgtaccatat tgaacgcact gaaattttcg
     3541 accaatggct tgaaaattta aaagatccac ttgctgtggt gaccattgca agacgtattg
     3601 aacgtgccga aagcggaaat tttggcgata ccaaatatat cgcagaagga atttccgaaa
     3661 tgagattaaa cgtcagcaaa ggttatcgcc tttactatgc acaaaaaggt agcattattt
     3721 atatactact aaatggagga tataaaggca cgccaagctg atattgataa agcataaaat
     3781 gctatgggca gaaattaaac agcatatggc agaacaggag aaaaagaatg aacgaattat
     3841 acgatgaccc gattccacaa gccactcgtg aaaaatatgg tatcaccgaa tttgatgccg
     3901 ccgaatacct caccaatgac gagctaatcg ccctttattt ggcggaaaca ttgaaagacg
     3961 gcacggacga ggagtttatt caggcattga ataccgtggc acgagcgaaa ggaatgaatg
     4021 agttagcgaa aaaagcaggg atcgggcgtg aaagtttgta tcaaagcctt tccagcagta
     4081 agccccgttt tgagactatc cgcaaaatta ttagcgcatt aaatttggag ttgtcggtcg
     4141 ttaaggcgta agtttgtgca ttaattgtat cccctgccaa accccgtggt cgaaacagta
     4201 ggtgtatttc atcggtagga caagcggtgg attttggctc attttttgcc atattttttg
     4261 taatgctggg tgtttgagca gttcggcttg gttgttatag ctttggttat cggtgaggaa
     4321 caataactgt ttgggttgcg ctaacagcag tttttccagc gagaaattct gtggcgtgag
     4381 gtcgcttttt aatggcgata gaccgagaag atgcaacaac gtttgatatt gcggaaaatg
     4441 cggttcgatc atacccgttt cgcttagaat taaagtttca gcaagcggtt gttttagcgg
     4501 aattttttgc aaacgtagcc gttccaccag cttttccgct ttggcttgat tttgggttag
     4561 ctcgcccagt tgtaaaatca gtgtgaaaag ctgttcgggt gtttgcggac tgtcgtttaa
     4621 tgcgactaac ttaaagccaa gctgtttcag ccgtgcggtg agttgcggat agaaggtttc
     4681 attcagtaaa atggtgctgt cggcataggg caacagggcg gtgagttcag gctcaacggt
     4741 gggcttgtcc gtgttcagtt tatccaacat catcaacggc ttttgggaat agggcgacat
     4801 cgcccgaatt tgctgtggtt cggcaagttc cagcaacaga cggtcgctac acaaatttgg
     4861 cgataaacgg taagctcctg aaatacgccc agtgatagaa tttttaaact gtgaattatc
     4921 ggtgtaacgg ccactaatag aaaaactgtg atcttgttcg gtaaataagc gatattctag
     4981 tgccgcactt ttttcggata aggttttttc acgcaaatat ttatcagagg tatagcgtgt
     5041 ttttgcataa tctgtcagta aactgaccgc ttgtgtcact tctccttcac ggtcaaagtt
     5101 tacatctaat tgataattcg cattaagttg tttttcgttg ctgtagcgag catttgaacc
     5161 gaaatgtgtt gctttactat tgaattggct gatatttgct tggtgtttca gcagctcttg
     5221 ttcatttccc caataaccgc ctaatttaaa agtttgattg cgagtacggg aatattcacc
     5281 gctaacgtct gaattatagc catcaatatg aacggtttgg gaagattgag cggcaagaac
     5341 ttctacccct ttgttaccga ggtcataacc taaacgtaaa gagccgttat cacgatgaaa
     5401 gccatctcgt tcgcttgcac caccggtttt cacttctgag cctgtttctg tggtatagct
     5461 gaaatggttt ttactcagtg cggaaatgcc tttggtacga tggctatcac cgtgtaaggc
     5521 atagtagaaa ccgttgtgaa atccggaaag tgttgcgctg gcatcacggg tattatttga
     5581 gcctaaacca agatcgacct ccgcattgaa tggtttctct ttgtataaac cgcttttagt
     5641 ggtgatataa actacaccac ccatagcact gcttccccaa agggcggatt gctcgccacg
     5701 caacacttcg atacgttcga tattgctaat cggtaagcca ccaaaatcga aatttccaat
     5761 attaatcgga ttgactttta caccgtcaat cacaaccgca gtatggcgag attctgcccc
     5821 tcgtaaaaat aagctagtaa gagtaccacg cccaccttgt tgcccaatcg ccacgcccgg
     5881 cacagtcttt aacacatcac tcacataagt cgcattgcgt gcggcaaaat ctttttcggt
     5941 aagaaccgtc acgctggaag cggttttgtc ttggttgatt ggggttgcat aggcggaata
     6001 gactgttacg gaatcaagct gagtcatctc attttgtgca aaagctgtac ttgctagtgc
     6061 aacagaaatg gcgaatgctg tgggattcta ttgatcgcct aagtccttta aaaccgaaat
     6121 ttgtttcggt aacttatgga gcaaattctg gtgagcgaga tcgtacacac agtattgtga
     6181 aatcaatcca agaaaaaaca gggatcattt cagctccgca cttaacaggc attgatgcaa
     6241 caccggaaga attaaaggta attgccaaag attattggga tagtggtatt cgccgaattg
     6301 ttgctttgcg tggcgatata ccgaaaggtt atgataaagc actattttac gcctcagatt
     6361 tagtcgcttt attaaaatca gtagcagatt tcgatatttc cgtggctgcc taccctgaag
     6421 ttcaccctga agcaaaatca gcacaggcag atttacttaa cttgaaacgt aaaatcgatg
     6481 cgggtgcaaa ccacgttatt acgcaattct tttttgatgt agagagttat ttacgttttc
     6541 gtgatcgctg tgtttctatt gggattgatt gtgaaattgt gccgggtatt ctgccggtga
     6601 caaatttcaa gcagttgcaa aaaatggcag cagtgactaa tgttaagatc ccgacttgga
     6661 tgggtaaaat gtacgaaggg ttagatgatg atcaaaccac cagaaactta gttgcagcaa
     6721 gtattgcaat ggatatggtg aaagtgcttt ctcgtgaagg ggtgaatgat ttccacttct
     6781 ataccttaaa tcgtagtgaa ctcacttatg caatgtgcca tactttaggc gtaagacctg
     6841 atttaacgac tgcataatcg aacattaagg ctgtatagct aaatgagcta tacctcctta
     6901 tagttttact tctcttcagc cacccaaatc tcgccatttt ttaataataa ataggctttg
     6961 ttggaatcgg ctttggtttg tttaattcca gctggcaggg cggttggcaa ttcagtgatc
     7021 ttgcctgttt tatccaacac aacaacttgt tgatagccca tttggggtat cagtgcgaaa
     7081 gtctctgttg tcgccactaa attggtttcg tgatcaccat agcggtgatt gcttaagccg
     7141 gaggtaataa aacgttcagc taattggttt cctttcaggc tgtattcaac caaattgcct
     7201 tttagatgtg gcatttggat tgcatagagc ttgcggttaa agataaacgg cgattgccaa
     7261 cggttagtcg acagcggctc actttcggct tctattgata attttccgtc tttaacgcca
     7321 atcacaacag tgctggcccc attgccatcg ccagagataa cagaaacgat cttggttttt
     7381 ccattttctg tgaaagtcgc ccagcggttg gcttcaaata ctcgcccttg ttgggtgagt
     7441 ttttctgcta aggggtttag ggtatggcgt tctagaaatt gaatttcgcc tgcctctaca
     7501 tcatcgccta atacaccgtg ttgatatgta aaatcatcag gctttgcaag tacagcaata
     7561 tgcccttggt taggtgtttc ggcatcaaaa tcaatttgca gagggtgagc atcaggcaac
     7621 acctcggttt ctgcggtggc agtaatagtg gcttggtttc cttgcgtttc aattctggct
     7681 aacttaaatt gtccgttttg tttggtcacg gcaatggtgg caaaattaag ggcggtcatt
     7741 gtagctttcg gggcaagggg aatgttggag gtatagattt tatcagccgt ccacagcata
     7801 aaatgcccgt tttgatccgc taacgcaatt cgttggtatt gagctttagg ttcaatctcg
     7861 gtggaaactc catctgccag tttttgagct tgttgtgtga ctctccataa ttgtttttgt
     7921 tggttaatta ccactaattc gccttgttct gaaaggtcaa attggcttac tttgccaatt
     7981 tggggaagtg agagtggaga aagctgaaca tcagcaaaca aagaagcgga gaacaagctt
     8041 aagattaaca gaatagattt tttcattagc gttacctaaa aagaagattt aaatgataaa
     8101 gggagatgtt atctcccttt acgctttagc ctttataacc attcgggtta tttttttgcc
     8161 aattccaagt gtctttcatc atttgttcta gcccacgctc ggttttccaa cccagttgct
     8221 ctaacgcttt ttgtggagcg gagtagcaaa cggcaatatc gcccgggcgg cgatctacca
     8281 ctttgtatgg aatagtaatg ccatttgcgg cttcaaaggc ttttaccata tctagcaccg
     8341 aataacctgt tcctgtgcct aaattgtaaa tgtggaaacc ggcatcattt tggtgcttgt
     8401 ctaaggcttt taagtgacca agtgctaaat ctaccacgtg gatataatca cgcacacctg
     8461 tgccatcgtg agtattataa tcgccaccaa acaccgataa ttgaggtaat ttgcccacag
     8521 ccacttggct gataaacggc attaagttat ttgggatacc gtttggatct tcaccgatta
     8581 aaccgctttc gtgagcccct accgggttga agtaacgcaa tactaccgca cttaattgtg
     8641 ggaaggcttt aacggtatct tcaagaatgc gttcaaccat atatttggac gtaccgtaag
     8701 ggttggttgt gccaccgaca gggcaagatt ccacaatcgg gataatctgc ggatcaccat
     8761 aaacggtcgc cgatgaacta aacacaatgg tattcacatt ggcttttagc atctcttcta
     8821 ccaatacaat cgagccggtt acattgtttt gatagtaacg tagcggttct ctgacgcttt
     8881 cgcctacagc ttttaaaccg gcaaagtgaa ttaccgattc aatctgattt tccgcaaaaa
     8941 ttttgcgtaa aatatcacga tctaaaatat cgccttgata gaattttacg ctcttgccgg
     9001 tgatctgctt cacacgctca agtgatactt cagaggaatt agaaagatta tctaacacca
     9061 cgatttcacg attttcgttt aataattcaa ctaatgtatg tgaaccgatg tagcctgcac
     9121 cacctgtaac taaaattgcc attttatgtt cctctataaa attaatacga accaaggtta
     9181 ttgttgctat tttacccgat ttaatcaggc ttgctaatat ttattcagtt tataatgcca
     9241 ctccggcttc cggcagtaat tccggaatgc cgtttttaat cgggtaagca atattgagct
     9301 tagggttaat taatcggttg ttctctttgt cccattctaa tttttcatta gagacagggc
     9361 agacgaggtt attgagtagt gtttcgttca tcttcttttc ctagtttttg tagaatcggg
     9421 gctaacaaac aagcggtcga tttttctgaa aatgttgcag aaaccggtac ataccaccaa
     9481 tttggctgtg caaaatcgtg gcattttacc gcatctttct cggtcattaa taacggcgtt
     9541 tggctatcag caaattgagc cagctgagcc ttatcaaagg cttgatgatc ggcaaagcct
     9601 tgagttttgg caagcggaat gcctaatttt tctaacatcg taaaaaagcg tggtgggtga
     9661 ccgataccgg caatcgccac acagggttgg gtaaattcgc ttaatggctt gcgttgattg
     9721 gtgactaaat taattgcatc agacggttca agcgtcatta aatgctcgtt agcttgcggt
     9781 gtgccgccgt tgcaaataac cgcttgcacg gagtttaaac ggctcggtaa ttcacgcaat
     9841 ccgcccgccg gcagcacaaa accgttaccg aaacgcctta cgccatcaac caccacccat
     9901 tccatat
//
LOCUS       DANKBV010000082         9675 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.082, whole genome shotgun sequence.
ACCESSION   DANKBV010000082 DANKBV010000000
VERSION     DANKBV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9675
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.082"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(18..698)
                     /gene="mutH"
                     /locus_tag="PWY43_001656"
     CDS             complement(18..698)
                     /gene="mutH"
                     /locus_tag="PWY43_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDL2136522.1"
                     /translation="MQLSTFSPTEQALLSKAEWLAGFTLGEIAEMLHIPIPADLKRDK
                     GWVGTLIETALGAKAGSKAEQDFAHLGIELKTIPVNQKGLPLETTFVSLAPLTQNNGI
                     TWETSHVKHKLSRVLWIPVEGERKIPLAERHIGQPILWSPTITQESRLQQDWEELMEL
                     IVLGELHKINATLGEVLQLRPKGRNNRSITSAINAKGEIVQSLPLGFYLRKHFTAEIL
                     QNFLHCPL"
     gene            complement(790..1425)
                     /gene="lexA"
                     /locus_tag="PWY43_001657"
     CDS             complement(790..1425)
                     /gene="lexA"
                     /locus_tag="PWY43_001657"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDL2136523.1"
                     /translation="MRKHLTARQQEIFDFVKNHIETTGMPPTRVEIAREIGFKSPNAA
                     EEHLKALARKGYIEMLSGTSRGIRILVEDENQAANDEDEGLPLIGKVAAGTPILAVEH
                     VENHYPVNGAMFNPSADYLLKVNGNSMEKIGILDGDLLAVHKTNTARNGQVVVARVND
                     EVTVKRLEKKGELIYLHPENDELKPIVVNPAQEYIEIEGIAVGVIRSNAWM"
     gene            1592..4027
                     /gene="plsB"
                     /locus_tag="PWY43_001658"
     CDS             1592..4027
                     /gene="plsB"
                     /locus_tag="PWY43_001658"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDL2136524.1"
                     /translation="MSGLLNFYRAFLNLPLSLLVKSRSIPTHPVAELELNLEQPIVYV
                     LPYTSQTDLLILQKNCLALNLPDPLQENVIEGQTLLRFVFLDEGRRFFKSKGAKSETE
                     SIFYRYLDLHRANAELDVQLVPVSVLWGRAPGKEDARHLQVLTSFQRFLSMVWFGRDN
                     FVRFSPAVSLRYMVTEYGADEKIAQKIARVAKMHFAKLRYSAMGPRLPNRDAMFNKIL
                     NSEVIQAAIAEEAKKSSPEKARKEAEKIINEIAADVKHESLRVADRVLSWLWNKLYQG
                     INVQNADRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFWP
                     AGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEP
                     KTGMMSMSLQALQRGLNRPLSIVPVYIGYEHVLEVDTYAKELRGAAKEKENAGLVLRV
                     LKKLKNLGQGYVNFGEPILVNNYLNQYFPEWKEPSEDGRPKWLNEAVDNISHQVMVNI
                     NKAAAVNAKNLVGSALLASRQRALTREQLIEQLESYIQLFRNVPYSKDMTLPTESAEV
                     MLNHVIHLPRSGVLIEKDNFGELVRLERESAVLMTYYRNNVQHLFVLPSLVASMVLHH
                     EAVSKDVVIKSVNRIYPFLQAELFLHFKQEELKAHIEAIIAELSRQGVIKNESDMLMI
                     NRSHIRTLQLHSAGVREILQRYYISLSVLLEQPNISRGSLEKESSSIAQRLSVLHGIN
                     APEFFDKAIFSTFTSSLKAQGYFDGDDNLAIAKVKETESLLRSLISIEVQQTIQGAMA
                     KVAESVENPNIAG"
     gene            complement(4132..4695)
                     /locus_tag="PWY43_001659"
     CDS             complement(4132..4695)
                     /locus_tag="PWY43_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDL2136525.1"
                     /translation="MSAPNSGTNPAPLGLCGFALTTFLLSLINNGTFSGENVGLVLAM
                     GFAFGGTAQMIAGMFEFAKGNTFGFTAFTSYGAFWWSFALFKVFFAANVSAEFVGWYL
                     VAWGTFTLMMFIGTLAKARALQAIFLSLTITFYLLAAGDFTHNTTLTNIGGNFGLLTA
                     FLAFYLAAADIINESFGKTVLPIGEPK"
     gene            complement(4893..5792)
                     /locus_tag="PWY43_001660"
     CDS             complement(4893..5792)
                     /locus_tag="PWY43_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2136526.1"
                     /translation="MDILDRLLAFAQISAGISVKCQLQGDWQLENPHQTGQAIAHIIS
                     YGQAYLQHNGKTHHLKQGDIVFFPRFADHTLRSQIQPLTSKNAVNLEQLGAFTLTQAG
                     LSQSKCDMFCLHFYYDHQAELMESLPEMIVFQVAETPLKAMLDILKQEAETPNLAVKS
                     VVNSLAVVILTMILREHLLVNHNNQLFGILKGWQDLRLRPLIKAILLNPEHEWQVDDM
                     AAFIHLSRAQLVRLFNKHLCISPHAFVHKIRLQKAAMLLKSQAGSVLSIALSCGFQSE
                     PHFITAFKKQYCVTPSQYRQLNP"
     gene            5928..6269
                     /locus_tag="PWY43_001661"
     CDS             5928..6269
                     /locus_tag="PWY43_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2136527.1"
                     /translation="MSSFQNWTEHTAHVKQSFGKLGQKHPKMLEAYKALGTAAAAEAL
                     DHKTRELIALAVAITTRCESCISVHAEEAAKAGATESEIAGALATAIALNAGAAYTYA
                     LRALEAVEEQK"
     gene            6280..6633
                     /locus_tag="PWY43_001662"
     CDS             6280..6633
                     /locus_tag="PWY43_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2136528.1"
                     /translation="MKKLAWILGFAAMMPMMSSAKQASVQQVQAEKAAGIWIDVRSAE
                     EFQQGHLNGAINVTHTEIAQRIAEVAPDKNQPIHLYCRSGHRAEAALNELKKLGYSNV
                     TNHGGYDDLIKAGLK"
     gene            6693..7331
                     /locus_tag="PWY43_001663"
     CDS             6693..7331
                     /locus_tag="PWY43_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgB family protein"
                     /protein_id="HDL2136529.1"
                     /translation="MSAFNNFVNFVANIVAPMQRQFINFVRIAICIVMVWIGGLKVCQ
                     YEADGIAHFVSNSPFFSYMYKKGPNLVDDGTGKMVMEYTLHKNPEGKMVAKNIEWHKE
                     NGTYTASYIIGATIVTVGLLTLSGIWFPVSGMAGGLLTFGMSIVTLSFMITTPEIWVP
                     NLGGDMPTPAHGFPYLSAVGRLIVKDVIMMAGGLVAAAECANRILARRKAIA"
     gene            7466..8647
                     /gene="purT"
                     /locus_tag="PWY43_001664"
     CDS             7466..8647
                     /gene="purT"
                     /locus_tag="PWY43_001664"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDL2136530.1"
                     /translation="MTTIGTPLRTNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRY
                     DNAPAQQVAHRAYTISMLDGNALRDLVEKEKPDFIVPEVEAIATATLVELEQEGYNVI
                     PTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNFEQFQQAIQEIGIPCVVKPIMSS
                     SGHGQSVIKSEADIQQAWDYSQQGGRAGGGRVIVEGFIKFDYEITQLTVRHIHGTSFL
                     APIGHIQVDGDYRESWQPQQMSGIALKKAQETAEKITSALGGRGIFGVELFVCGDEII
                     FNEVSPRPHDTGIVTMASQELSQFALHARAILGLPIPEIYRISPAASKAIVVEGKSDN
                     VRFGGVDKVLAEIGTNIRLFGKGEVNGHRRLGVILARDENTVRALEKARRAYDKLDIQ
                     L"
     gene            complement(8739..>9675)
                     /locus_tag="PWY43_001665"
     CDS             complement(8739..>9675)
                     /locus_tag="PWY43_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2136531.1"
                     /translation="GIAEMGEIGTFFYGFLMRLSGAVGLHHMIYPLFWYTELGGTEVV
                     NGETIIGAQKIFFAQLADPTHQGLFTEGTRFFAGRFDTMMFGLPAACLAMYHAVPKAR
                     RGAISGLFLGAALTSFITGITEPIEFMFLFVAPWLYVFHAFLDGVSFYIADLLNISIG
                     NTFSGGVIDFTLFGILQGNENTNWLRVIIVGLPWAALYYFSFLFLIKKFNVMTPGRAE
                     ESEEVVEQQTSSLTENAHKIIAALGSANNIENVDACITRLRVAVKEVKAVDKATLKKL
                     GAIDVLEVGGGVQAVFGAKAVLYKSEVNQILGKED"
BASE COUNT         2828 a         1844 c         2154 g         2849 t
ORIGIN      
        1 agagtgtgag gacaaattta aagggggcaa tgcaaaaagt tttgtaaaat ttcggcggta
       61 aaatgtttac gcaaatagaa accaagaggg agagattgaa caatctcccc tttagcgttg
      121 atagcagagg tgatagagcg gttatttcta cctttgggtc tgagttgtaa cacttcgcct
      181 aaggttgcat taattttatg cagttcacct aacacaatca actccattaa ttcctcccaa
      241 tcttgctgta ggcgggattc ttgtgtgata gtcggagacc ataaaatcgg ctgaccgata
      301 tggcgttcgg caaggggtat ctttcgttcc ccttcaaccg gaatccaaag cacacgggaa
      361 agcttatgtt taacgtggga ggtttcccaa gtaataccgt tgttttgggt taaaggagca
      421 aggctgacga atgtggtttc cagcggtaat cctttctgat tgaccggaat ggttttcagc
      481 tcaatgccta aatgtgcaaa atcctgttcg gctttactgc cggctttcgc acctaaagcg
      541 gtttcaatca gagtaccgac ccagccttta tctcgtttca gatctgctgg tatcggaata
      601 tgaagcatct cggcaatttc acctaaagtg aaccctgcca gccattccgc tttgcttaat
      661 aatgcctgct cggttggaga gaatgtagat aattgcattg cacttagtta attagactaa
      721 actggcaagc ggtcaaattt aggcagaaat ttgcaaaaaa ttactaaaaa ataaccgctt
      781 gtgaggaaat tacatccaag cattactgcg aataacgcct actgcaatgc cttcaatttc
      841 aatatattct tgagcagggt ttactacaat cggttttaat tcatcgtttt cagggtgtaa
      901 gtaaattaat tcacctttct tctctaagcg tttaacagtc acttcatcat ttacccgagc
      961 aaccaccact tgcccgttac gagcggtatt ggttttatga acggctaata ggtcaccgtc
     1021 taaaataccg attttttcca ttgaattgcc atttactttg agtaaataat cagcacttgg
     1081 attaaacatc gcaccattca ccgggtaatg gttttcaacg tgttcaaccg ctaaaatagg
     1141 tgtgccagca gctactttgc caatcaacgg taagccttca tcttcatcgt tggcagcttg
     1201 gttttcgtct tcgactaaaa tgcgaattcc acgagatgta cctgataaca tttcaatata
     1261 gcctttacga gcaagggctt ttaagtgttc ttctgccgca ttaggcgatt taaagccgat
     1321 ttcacgggct atttctactc gggttggtgg cataccggtg gtttcaatat ggtttttgac
     1381 gaaatcaaaa atttcttgct gacgggcagt tagatgttta cgcattttaa gttaatcctc
     1441 actgttttta tatacagaaa atattgatag tatatacagt atttttgatt ttgtaactaa
     1501 taatttcacg aaattccata aaaatttatg gtagaatgga agaaattaga ggttagacct
     1561 ccaacaataa cgattctaaa aggatattta tatgtctggc ttattaaatt tttatcgagc
     1621 tttcctaaac ttaccacttt cacttttagt aaaatctcgc tctattccga ctcatccggt
     1681 ggctgaactg gaactaaatc ttgaacagcc gattgtctat gttctacctt atacctcgca
     1741 aaccgattta ttaattttac aaaaaaactg cttggctttg aatttgcccg atccgttgca
     1801 agaaaatgtg attgaagggc aaacattgct tcgttttgta tttttagatg aaggtcgccg
     1861 tttttttaaa tctaaagggg cgaaaagtga gacagagtcg attttttatc gttatttaga
     1921 tttacatcgt gctaatgctg aattagacgt ccaattagtg ccggtttcgg tattatgggg
     1981 gcgggctccg ggcaaagaag atgcccgcca tttacaggtg cttacctcat tccaacgttt
     2041 tttatccatg gtttggtttg ggcgtgataa ttttgtacgt ttctcccctg cagtatcgtt
     2101 acgctatatg gtaactgaat atggtgcaga tgaaaaaatc gctcaaaaaa tcgcgcgtgt
     2161 ggcgaaaatg cactttgcaa aattgcgtta ttcagcaatg gggcctcgtt taccaaatcg
     2221 tgatgcgatg tttaataaaa tcctgaattc agaggtgatt caagctgcga ttgcggaaga
     2281 agcgaagaaa tcatcgcctg aaaaggctcg caaagaagcc gagaaaatca ttaatgaaat
     2341 tgccgctgat gtaaaacacg agagtttgcg tgtagcagat cgagttctga gctggctttg
     2401 gaataagctt tatcaaggga tcaacgtgca aaatgcagac cgtgtgcgta aattggcatt
     2461 agaaggtcac gagatcgttt atgtgccttg ccaccgtagc catatggact atttgctcct
     2521 atcttatcta ctttaccatc aaggattagt accgccacat attgccgctg ggatcaattt
     2581 aaatttctgg ccggcaggcc cgattttccg tagttgggga gcatttttca ttcgccgtac
     2641 ctttaaagga aatcgcctct attccaccat tttccgtgaa tatttagcgg aattattcta
     2701 tcgtggttat tcggtcgaat attttattga aggcgggcgt tcacgcacag gtcgtttatt
     2761 agagcctaaa accggtatga tgtcgatgag cctacaagcc ttacagcgag ggctgaatag
     2821 acctttaagt attgtgcctg tttatatcgg ttatgaacac gtcttagaag tcgatactta
     2881 cgctaaagag ttgagaggtg cggcaaaaga gaaagaaaat gcgggcttgg tgctacgagt
     2941 gctgaaaaaa ctgaaaaatt taggtcaagg atatgtgaat ttcggtgagc caattttggt
     3001 gaataattac ttaaatcaat atttccctga gtggaaagag ccttccgaag atggacgacc
     3061 taagtggtta aatgaagccg tggataatat ttcacatcaa gtaatggtga atatcaataa
     3121 agcggcagca gttaatgcga aaaacttagt ggggtcagca ttattggcat ctcgccagcg
     3181 tgcattaacc cgtgaacaac tcattgaaca acttgaaagt tatattcagt tatttcgtaa
     3241 tgtaccttat tctaaagata tgacattacc aactgaaagt gcagaagtga tgttgaatca
     3301 tgttattcat ttacctcgtt cgggcgtttt aattgaaaaa gataattttg gtgaactggt
     3361 tcgtttagaa cgagaatctg ctgtgttaat gacatattac agaaataatg tgcagcattt
     3421 atttgtgttg ccttcacttg ttgcaagtat ggtacttcat catgaggctg tttcgaaaga
     3481 tgttgtgatt aagtcggtta atcgtattta tccattcttg caagcagagt tattcttaca
     3541 ctttaagcaa gaagaattaa aagcacatat tgaagcaatt attgcggaac taagtcggca
     3601 gggggtgatt aaaaacgaaa gtgatatgtt aatgatcaat cgctcacata ttcgtacctt
     3661 gcaattacat tcagctggtg tgcgtgaaat ccttcaacgt tattatatta gcctaagtgt
     3721 cttgttggag cagccaaata ttagtcgtgg ctctttagaa aaagagagta gttcaattgc
     3781 tcagcgttta tcagtcttgc atggtattaa tgcgccggag ttttttgata aggcgatttt
     3841 ctctaccttt acatcaagtc taaaagcaca agggtatttt gatggtgatg ataatttagc
     3901 gattgcaaaa gttaaagaaa cggaatcgtt gttacgtagt ttgatttcga ttgaagttca
     3961 acagaccatt caaggtgcga tggctaaagt cgcagaatct gtagagaatc ctaatatcgc
     4021 aggataataa atcaaagaag ggcgctaaca agcgctcgtt tcttgcaatt ttgttgccaa
     4081 aaataaaagc tgaatgcgcg accacattca gcttttttac ttcctatata attattttgg
     4141 ttcaccgatt ggtaaaacag tcttaccaaa actttcatta ataatatccg cagcggctaa
     4201 gtagaacgct aagaatgcgg ttaataaacc gaagttacca ccaatgttag ttagggtggt
     4261 gttatgggta aaatcacctg cggctaaaag gtagaaagta atggttaaac ttaagaaaat
     4321 agcttgtaat gcacgggctt ttgctagtgt gccgatgaac atcataagtg tgaatgttcc
     4381 ccaagctact aagtaccaac caacgaattc tgccgataca ttcgcagcaa agaacacttt
     4441 aaataatgca aaagaccacc agaatgcacc atagctggta aacgcagtaa agccgaacgt
     4501 gttaccttta gcaaattcaa acatacctgc aatcatttgt gctgtgccac cgaaagcaaa
     4561 tcccattgct aaaactaaac ctacgttttc accgctgaat gtaccgttat tgattaggct
     4621 tagtaaaaat gtagtaagtg caaaaccgca taaaccgagc ggagccgggt tagttcctga
     4681 atttggagct gacattgaaa acctctgtta tattgttaca ccataagtgt ggtaattgtt
     4741 tatctacgcc gataaatcag acgttatgtg cctaaaattt agggcgataa agtatagcga
     4801 ttttgcagta aattgactag aaatttccga gacaagcggt gagatttttg caaaattttg
     4861 ccaaaaaatg accgcttgtt gttgtcattt agctatggat ttaactgccg atattggctg
     4921 ggtgttacgc aatattgctt tttaaaggcg gtaataaaat gcggctcgga ttggaagccg
     4981 caagataagg cgatacttaa taccgaccca gcttggcttt tgagcagcat tgctgctttt
     5041 tgtagcctaa ttttgtgtac aaaagcatgt gggctaatac ataaatgttt gttaaataag
     5101 cggactaact gggcacgaga aaggtggata aatgccgcca tatcatcgac ttgccattcg
     5161 tgttcgggat taagcaaaat agctttgatc aaaggtctta agcgtaagtc ttgccaacct
     5221 tttaaaatac cgaataactg attgttatgg ttaactagaa gatgttctcg taagatcata
     5281 gttaaaataa caacagcaag cgagttaacc acagatttta cggctaaatt aggtgtttct
     5341 gcttcttgtt ttagaatatc taacattgct tttaatggtg tctcggcaac ttgaaatacg
     5401 atcatttcag gtaagctttc cattaattcg gcttgatggt cataataaaa atggaggcag
     5461 aacatatcgc attttgattg agaaagtcct gcttgggtga gtgtaaatgc acccaattgt
     5521 tcaaggttta ccgcattttt gctggtcaaa ggctgtattt gactacgtaa tgtatgatcg
     5581 gcaaatctgg ggaaaaatac aatatctccc tgtttaaggt gatgggtttt cccattatgt
     5641 tgtagataag cttgcccata gctgatgata tgagcgattg cttgtccggt ttgatgcgga
     5701 ttctcaagtt gccaatctcc ctgtaactga catttcacac tgattccggc tgagatttga
     5761 gcgaaagcta gaagcctatc taatatatcc attgttcatt ccttatattt ccaccttaaa
     5821 ttgcgattat ttcattataa tatgagctga tttgataata aagtttatca gataaacgca
     5881 ataataaatg cgttttttca tattattcac tttaggagga aatcaatatg tcttcattcc
     5941 aaaattggac agaacacaca gctcacgtta agcaatcttt tggtaaatta gggcaaaaac
     6001 accctaaaat gcttgaagct tataaagcat taggtacagc ggccgcagcg gaagcattag
     6061 atcacaaaac tcgtgaatta attgcattag cagtagcgat tacaacacgt tgtgaaagtt
     6121 gcatcagtgt acacgcagaa gaagcggcaa aagcaggtgc aaccgaaagc gaaattgccg
     6181 gtgcattagc gactgcgatt gcattaaatg ccggtgcggc atatacctat gctttacgtg
     6241 cattagaagc ggtggaagag caaaaataag gagcaataaa tgaagaaatt ggcttggatt
     6301 ttaggctttg cagctatgat gccaatgatg tctagtgcta aacaagcatc agttcagcaa
     6361 gtgcaggctg aaaaagcagc gggcatttgg attgatgtac gttctgctga agaatttcag
     6421 caagggcatt taaatggtgc aattaatgta acgcatactg aaattgcaca acgtatcgca
     6481 gaagttgctc cagataaaaa tcagccgatt catctttatt gccgctcggg tcatcgagct
     6541 gaagcggcgc ttaatgagct aaaaaaatta ggttacagca atgtaactaa tcatggtggt
     6601 tatgacgatc tgattaaagc cgggttgaaa taactgtttt gagtgccaag catttgcttg
     6661 gcacttttcg tttatatctg aggttaaaaa ttatgagtgc ttttaacaat tttgttaatt
     6721 ttgttgcaaa tattgttgct ccaatgcaac gccaattcat taattttgtg cgtattgcta
     6781 tttgtattgt gatggtttgg attggtggtt taaaagtctg ccaatatgaa gcagacggta
     6841 ttgcccattt tgtatcgaat agcccattct ttagttatat gtacaaaaaa ggcccgaact
     6901 tagtcgatga tggcacaggt aaaatggtga tggaatacac ccttcacaaa aatcctgaag
     6961 gcaaaatggt ggcaaaaaac atcgaatggc ataaagaaaa tggcacttat accgcttctt
     7021 atatcatcgg tgcaactatt gtgacagttg gtttattaac cttatccggc atttggttcc
     7081 cagtttccgg tatggctggg ggcttactca cttttggtat gtcgattgtt acactctcgt
     7141 ttatgattac cacccctgaa atttgggtgc cgaatttagg tggggatatg ccaacccctg
     7201 cacacggttt cccatatctg tcggcagtcg gtcgtttaat tgtaaaagat gtgattatga
     7261 tggcaggcgg cttggtggcc gcagcggaat gtgcaaatcg cattttagcc agacgtaaag
     7321 cgattgctta attaatagtg ctaatgcaag cggtcatttt cttgtaaaat tttgcaataa
     7381 aatgaccgct tgttttttgt caatttgcca taatttcaag tataatccaa ttacgcaatc
     7441 gtttgctttt atggagagga ataaaatgac aacaatcggt acaccgttaa gaacgaacgc
     7501 tacaaaagtg atgatgttag gttcgggcga gttggggaaa gaagtggtaa tagagctgca
     7561 acgcttaggc gtagaagtga ttgcggtgga tcgttatgac aatgcacctg cacagcaagt
     7621 ggctcaccgt gcttatacga tttcgatgtt agacgggaat gcattgcgag atttggtgga
     7681 gaaagaaaaa ccggatttta ttgtgcccga agtagaagcc atcgcaactg caaccttggt
     7741 tgagttagag caagagggat ataacgtaat cccaacggca aaagcaacgc aattaacaat
     7801 gaatcgagaa ggcattcgcc gattggcggc agaagagttg ggcttaaaaa cgtcgcctta
     7861 tcgttttgtg gataattttg aacagttcca gcaagcgatt caagaaatcg gcattccgtg
     7921 tgtggtcaaa ccgattatgt cttcttccgg acacgggcaa tcagtaatta aatctgaagc
     7981 ggatattcaa caggcttggg attattcgca acagggcgga cgtgccggcg gtgggcgtgt
     8041 gattgtagag ggttttatca aattcgatta tgaaattacc cagcttaccg ttcgccatat
     8101 tcacggcacg tcgtttttag ctcccatcgg gcatattcag gttgatggcg attatcgtga
     8161 atcttggcaa ccgcagcaaa tgtcgggcat tgcattgaaa aaagctcaag aaaccgcaga
     8221 aaaaattacc tctgcactgg gcggaagagg gattttcggc gtggaacttt ttgtctgtgg
     8281 cgatgagatt atttttaacg aagtctcccc acgtccacac gataccggaa tagtaacaat
     8341 ggcatcacaa gagctttctc aatttgctct gcacgcccgt gcgattttgg gcttaccgat
     8401 tccggaaatt taccgcatca gcccggcagc ttctaaagcg attgtggttg aagggaaatc
     8461 ggataatgtg cgttttggcg gtgtagataa ggtattagcc gaaatcggga cgaatatccg
     8521 cttattcggt aaaggcgaag taaacggaca ccgccgatta ggggtaattc ttgctcgtga
     8581 tgaaaataca gtgagagcat tagaaaaagc tcggcgagct tatgataagt tagatattca
     8641 attataaagt caggattttg atattcgttg atagtaggca ttttatgcct actatcctat
     8701 aggcctctaa tcaaattgga tgaaaaagtt gaagattgtt aatcctcttt gccgagaatt
     8761 tggttcactt cgctcttata caatactgct tttgctccaa acacggcttg tacaccgcca
     8821 ccgacttcca gcacgtcaat cgcaccgagt tttttcagtg ttgctttatc cacggctttc
     8881 acctctttca ctgccacacg cagacgggta atacaagcgt ccacattttc gatattattt
     8941 gcagagccaa gagcggcgat aattttatgg gcattttcag tcagtgagct ggtttgttgt
     9001 tcgactacct cttcactctc ttccgcacgt cccggtgtca ttacgttgaa ttttttgatc
     9061 aggaatagga acgagaagta gtaaagtgcc gcccacggca agccgactat gatcactcga
     9121 agccagttag tgttctcgtt gccttgcaaa atgccaaaca gggtgaagtc gatcacgcca
     9181 ccggagaagg tgttaccaat cgagatgttg agtaagtctg caatgtagaa cgacacgccg
     9241 tctaaaaaag cgtggaacac atagagccac ggggcaacaa acaggaacat aaattcaatc
     9301 ggctcggtaa tgccggtgat aaaagaggtg agcgctgcac ctaagaacag accgctaatc
     9361 gcaccacggc gggctttcgg cacagcgtga tacatcgcta aacaggcggc aggtaagcca
     9421 aacatcatcg tatcgaaacg tccagcgaag aaacgtgtac cttcggtaaa taagccttga
     9481 tgggttggat cggcgagttg ggcgaagaag attttttgcg ccccgataat ggtttcacca
     9541 tttaccactt ctgttccgcc caattcggtg taccaaaaca gcgggtagat catatggtgt
     9601 aagccgaccg caccgcttaa ccgcattaag aaaccgtaga aaaaggtgcc gatttcgccc
     9661 atttccgcaa tccct
//
LOCUS       DANKBV010000083         9524 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.083, whole genome shotgun sequence.
ACCESSION   DANKBV010000083 DANKBV010000000
VERSION     DANKBV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.083"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            111..827
                     /gene="rph"
                     /locus_tag="PWY43_001666"
     CDS             111..827
                     /gene="rph"
                     /locus_tag="PWY43_001666"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL2136532.1"
                     /translation="MRPNNRANNQVREIKITRNYTRYAEGSVLIEFGETKVLCNATVE
                     ESVPRFLKGQQQGWVTAEYGMLPRATHSRTQREAAKGKQGGRTMEIQRLIARSLRAVV
                     DLKALGERTVTVDCDVIQADGGTRTASITGACVALYDAMNKLVADGTLKTNPMKGLVA
                     AISVGIVAGEAVCDLEYLEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHQELLTLL
                     DLAKQGISQLVEAQRKALAE"
     gene            1051..3350
                     /locus_tag="PWY43_001667"
                     /pseudo
     CDS             1051..3350
                     /locus_tag="PWY43_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(3428..4129)
                     /locus_tag="PWY43_001668"
     CDS             complement(3428..4129)
                     /locus_tag="PWY43_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDL2136533.1"
                     /translation="MDLQNRENFLNKLAQRMGRPRQNVPEPIPVLENNHAITRLTDRP
                     QPQLCQEFIDFARVMMADVVEAKEADLPQAIIDICEKYGGASVIVNNDQRLKDLGITA
                     AIQAKYEDSYVWDFNQAETNFEKARTANIGVVYGEYGLAESGGIVLFSDKEFGRSVSL
                     LPEKSVVVLRKSTVLPRVAQLAQILHEKAQRGERMPSCVNIISGPSATADIELIKVVG
                     VHGPVNKIYLVIDDL"
     gene            complement(4134..5543)
                     /locus_tag="PWY43_001669"
     CDS             complement(4134..5543)
                     /locus_tag="PWY43_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDL2136534.1"
                     /translation="MSYLQTNTLPFKQRVEQQVNNEIVRKALVKAQETIGANRQRMVD
                     ELGNWEEWRDDAKAIRNHVLANLDAYLYQLSEKVTQNGGHVFFAETAEEATDYIKNVA
                     KAKNAKKIVKSKSMVTEEIGMNHVLEAEGIKVVETDLGEYLLQIVGDKPSHIVVPAIH
                     KDRHRIRQEMHDVLGYQGSETPEEMTAFVRQKIREDFLEADIGISGCNFAVPETGSVC
                     LVTNEGNLRMATTVPKTHIAVMGMERIAPTFKEVDVLITMLARSAVGAKLTAYNTWLT
                     GPRLEGETDGPEEFHLVIVDNGRSKILESEFQEVLRCIRCGACLNTCPAYRQIGGHGY
                     GSIYPGPIGSVISPLLGGYEEFKELPYACSLCTACNSVCPVKIPLAQLILKHREKIAD
                     AGLTPMAERLSIFGFNFANSHPTLWKVGVKVGAKVASKLIKNGKAPVNFGALGEWTKA
                     RDLPTAEGESFREWFNNRG"
     gene            complement(5545..6276)
                     /locus_tag="PWY43_001670"
     CDS             complement(5545..6276)
                     /locus_tag="PWY43_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDL2136535.1"
                     /translation="MNVNFYVTCIADVMKAGVAKNTVLLLEKLGCKITFLEKQGCCGQ
                     PAINSGYTKQALAGMKNLVETFEANDYPIVAPAGSCVYAIKTYPDHFERFGEKEWAER
                     AKKVADRFYDLTDFIVNKLGVKDVGAYLPGKAVYHPSCSLFRKLGIKDEPIALLQHVK
                     GLELLPIKNQETCCGFGGTFSVKMAEISGEMVKEKVANVDTDEPDYLIGADVSCLMNI
                     GGRLSREGKPIKVMHIAEVLMQGGK"
     gene            complement(6420..8024)
                     /locus_tag="PWY43_001671"
     CDS             complement(6420..8024)
                     /locus_tag="PWY43_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL2136536.1"
                     /translation="MALFLSVFPIVLLIYLMVKRNALPSYVALPLTALLIFVLQLTYF
                     GNDTTLIFANIIAGLGDVLTPITVIFGAILFNRFSEVSGATNTMRKWLGTINPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILVGLGFKPLQVAMLALVMNSVPVSFGAVGT
                     PTWFGMGPLLKDGLLTDAQVLEIGSITALIHSIAAFIIPVMALRLIVSWKEIRQNIVF
                     IYISIFACVIPYFIIAQFNYEFPSLVAGAIGLLVSVWVANMGIGLAKSENHLDGDKAT
                     FGEVAKALLPTGLLIFILVITRIQQLPLKAMLNDATAWIVSSLGFANFEISQGLIFAL
                     KNILGTNVATSYKLLYVPALIPFVVTVLICLPIFSVSGKNTQEIFGTSFKQVKKPFLA
                     LLGAIVMVKLMLIGGVDSMVQIIGKNFAEFTGQSWTLFASFLGAIGAFFSGSNTVSNL
                     TFGTVQYATAQQVGLSVTLILALQSVGGAMGNMVCINNIVAVCSVTGIEKQEGNIIKK
                     TAVPMFVYGVIAAISALFIVPMFYNL"
     gene            complement(8315..9406)
                     /gene="ychF"
                     /locus_tag="PWY43_001672"
     CDS             complement(8315..9406)
                     /gene="ychF"
                     /locus_tag="PWY43_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL2136537.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNTGVV
                     PMPDPRLDALAEIVKPERVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGH
                     VVRCFENDDIVHVAGKIDPADDIEIINTELALADLDSCERAIQRLQKRAKGGDKDAKF
                     ELSIMEKILPVLENAGMIRSVDLDKDELHAIKGYNFLTLKPTMYIANVNEDGFENNPY
                     LDRVREIAEKEGAVVVPVCAAIESEIAELDDEEKVEFLQDLGIEEPGLNRVIRAGYRL
                     LNLQTYFTAGVKEVRAWTVSVGATAPKAAAVIHTDFEKGFIRAEVIAYQDFIDNKGEA
                     GAKEAGKWRLEGKDYIVQDGDVMHFRFNV"
BASE COUNT         2897 a         2081 c         1813 g         2733 t
ORIGIN      
        1 gtccctctcc cacaaggggc gagggctatg taaattcttt gctaaagaaa aatcacattt
       61 tactgtgttc tttgctaaaa tggtaaaaat tttagcaaaa ggaaaagaaa atgcgtccaa
      121 ataatcgagc aaacaaccaa gttagagaaa ttaaaattac ccgtaactac actcgctatg
      181 ccgaaggctc ggtattaatt gagtttggcg aaaccaaagt attgtgtaac gctacggtag
      241 aagaaagcgt gccacgcttt ttaaaaggtc agcaacaagg ttgggttacc gcagaatatg
      301 gtatgttgcc acgagccacc cactctcgca ctcagcgtga agcggcaaaa ggcaaacaag
      361 gcggacgcac aatggaaatc caacgcttga ttgcccgttc tctgcgtgcg gtggtagatt
      421 taaaagcctt aggcgaacgt accgttaccg tagattgtga tgttattcaa gccgatggag
      481 gcactcgcac cgcctcaatt accggtgcgt gcgtagcatt gtacgatgca atgaataagt
      541 tagtagctga tggaacacta aaaactaacc caatgaaagg tttagtcgca gcaatttctg
      601 tcggtattgt agcaggcgaa gcggtgtgcg atttagaata tctggaagat tccaacgctg
      661 aaactgatat gaacgtggtg atggtcgaag atggtcgttt agtggaagta caaggcacgg
      721 cagaaggcga accgttctcg caccaagaat tactgacatt gctagattta gcgaagcaag
      781 gcattagcca gctagttgaa gctcaacgta aagcgttagc tgaataattc acttctcttt
      841 ttgattttct ttttaaataa aatctacatc tgacaaaaca gaataaccgt tctgattcag
      901 atgtagattt ttttatttcc cttttcaaaa tacactcaat cataaaataa caattatttt
      961 tttatggaaa tattgcactt cttacaaaat atctgtaaat ttcattgcca ttattttttt
     1021 atggctattt attctagaag gactctaaac gtgttaaaga aaaattatct cactgtatca
     1081 attctccttg ccattagtgg agtaggctac gctaatgaaa tctctttaga gactattact
     1141 gttgatggaa atacgccttc aaccaaaggg aaattactag gtggtgaact aaatagcaat
     1201 gaatctgttg ttgatgaaaa aaattaaaac aaggtagtat tacattaggt aacgctttat
     1261 ctggtgaatt aggcattcat tcaagccagt tcggaggcgg tgcaagtacc ccaattattc
     1321 gtgggcaaga gagtaaacgt gcaaaaatct tacaaaataa tggcgaaaac ttagatatgt
     1381 cgggaatgtc tcctgatcat gcagtaaccg tagatgcgct acttgctaaa cgtattgaaa
     1441 ttttacgtga tccaaccact ttactttaca gtgcaggtaa tacggcaggt gtaattaatg
     1501 tggtagacaa taaaattcca accgctattc ccgaaaaagg ctatgagggg caatttggtg
     1561 tgcgttttgg ctcagccagt aaagagcgtt taacctatgc aggctcaacc tttgcccttg
     1621 gaaatcattt agccttgcgg gtacagggaa tgtataacaa agcttccgaa tactatgctc
     1681 cacactttac gattgaaggc aaaccttacc accgcgtacc agatagcgat gtacaatcac
     1741 aaaccggcac agtaggcctt tcttggattg gagagcgagg acatcttggt attgcttaca
     1801 ctgatcgccg tgataaatat ggattaattg gtcacacaca caaatacgat cactatacta
     1861 ttagtattat tcgccaagcc gttatgtttg caaaaggtta tttacgtttt tatccgcacc
     1921 ttgcagaaga aggagatatt gactataaca accctggtat ccgtttactg catactcata
     1981 ttccgggagg ctcacattat ggacaagata ctcacgagca cggcaaacct tggattgata
     2041 tgcactccaa gcggtatgat attgatggaa gtttgcaaaa tccacttcct ggttttgaag
     2101 aagccaaaat atcagctaac tatgtagatt actatcatga tgaaaaagat ggaaaaagag
     2161 ttgaaaatta cttcaagaac aaaggaaaaa atctacgttt cgaattggtt cacaaagagt
     2221 ggaaagggct aaaaggagct attggtgtgc aatataccaa ccaatctacc tcagcacttg
     2281 cacttgaagc ttcacgagct gcaaaagtat ttaataaaca gcctttactc aataatccaa
     2341 aaacaaaatt atggagtctc tttgcgattg aacgccttaa tttaggtgac tttacctttg
     2401 agttaagtgg gcgggcagaa cgtcaaaaaa tagcaatgga ttatgatgtg aaattaattg
     2461 atcgctggct aggctttaat acacctatgc caaacttgga cccacataaa gacaaaggtt
     2521 attcttatag ctttgcaact cattggtatt tcgcaccaaa ccataagtta acattaaatg
     2581 ctgcacatca agaacgttta cctaatgctc aggaactcta tgctcatggt aaacacattg
     2641 ctttaaatgc atttgaggct ggcaataaaa atctagaaaa agagcgttct aaccaaattg
     2701 agctatcgtt agcttatgtt ggcgacaaat gggattataa attaaatctc taccacaccc
     2761 gctatggcaa ctatatttac ccattaactc ttaatgataa tcgtggacct aaatctttta
     2821 ccgatgaata taacttgaaa gtgaatagat attatcaagg cgaagctcgt tttagtgggg
     2881 cagaaggcga aattggttat ctatttacac caaattatcg cttagccgta ttcggagact
     2941 atgtccgtgg aaagttagtc aacttaccta atattgcaat gagttataac atttggacag
     3001 gtgaagttga caaatgggct tctcagccag acatttctgc acctcgtata ccaccactac
     3061 gtttaggagc aagatttaat gctgacttca acctaaactg gtctggaatg ttagaatatt
     3121 accgagtatt tgctcagaaa aaagtctcga aatacgaaca agttacccct ggccatcatc
     3181 aagtaaacct tggcgtaacc tatagtaatc attttaatca aacagaatac caagtattcc
     3241 ttaaagtgga taacttgctt aatcagaaaa tgtaccaaca tgcctcatat ctaccacata
     3301 ttccacaaat gggtagaaat gcaatgcttg gaatgaatat cagtttttaa tttgtaaatc
     3361 tctacaaaaa tataaccgct tgtaaatttc tatctacaag cggttacttt cagtggattt
     3421 tttgcaatta gagatcgtca attaccaagt aaattttatt taccgggccg tgaacgccaa
     3481 ctactttaat caactcaata tccgcagtcg ccgatgggcc tgagatgata ttcacacaag
     3541 atggcattct ttcacctcgt tgggcttttt cgtgcagaat ttgtgcaagc tgtgccacac
     3601 gaggtaacac tgtacttttg cgtaacacaa ccaccgattt ttcgggcaat aagctcactg
     3661 aacgcccaaa ttctttatcg gaaaacagta caataccgcc agactctgcc aagccatatt
     3721 caccgtaaac cacaccgata ttagcagttc tggctttttc aaagttggtt tcagcttggt
     3781 tgaaatccca cacatagctg tcttcgtatt ttgcttgaat ggctgcggta atcccaagat
     3841 cttttaaacg ttgatcgtta ttcacgatca cactcgcccc gccatatttt tcacaaatat
     3901 caataatggc ttgtggtaaa tccgcctctt ttgcctctac tacatctgcc atcatcactc
     3961 gggcaaaatc gataaactct tggcaaagtt gtggttgagg gcgatcggtt aaacgggtaa
     4021 tcgcgtggtt gttttccaac acaggaattg gctcaggcac attttggcgt ggtctgccca
     4081 tacgctgtgc caatttattt aagaaatttt cacgattttg taaatccatt ttcttatcct
     4141 ctgttattaa accattcacg gaaactttcg ccttcggcag tcggtaaatc acgggctttc
     4201 gtccattcac ctaacgcccc aaaattcacc ggtgctttgc cgtttttaat caatttgctt
     4261 gccactttcg cccccacttt tacgccgact ttccaaaggg ttgggtgtga gttagcgaag
     4321 ttaaagccaa aaatagacaa gcgttctgcc attggcgtta agccggcatc cgcaattttc
     4381 tcacggtgtt ttaagataag ctgtgccagt ggaattttca ctggacaaac actgttacaa
     4441 gcggtacaaa gtgagcaagc atacggcaat tctttaaact cttcgtagcc accgagcaat
     4501 ggcgaaataa ctgaaccaat cgggcccgga tagattgaac catagccgtg accgccaatt
     4561 tggcgatacg ccgggcaagt gttcaaacat gcaccacagc ggatacaacg caacacttct
     4621 tggaattcgc tttctaagat tttagaacga ccgttatcga caatgaccaa gtggaactct
     4681 tccggaccat cggtttcgcc ttcaagtcta ggacctgtca gccaagtgtt gtaagcggtg
     4741 agttttgccc ctaccgcact gcgagccagc atggtaatta atacatcaac ctctttaaag
     4801 gttggggcga tacgttccat acccattact gcaatatgcg ttttcggcac agttgttgcc
     4861 atacgcagat taccttcgtt ggtcactaaa caaaccgagc cggtttccgg caccgcaaag
     4921 ttacagcctg aaataccgat gtctgcctct aagaaatctt cacggatttt ttggcggacg
     4981 aatgcagtca tctcttcagg tgtttccgaa ccttgataac ctagcacatc gtgcatttct
     5041 tggcggattc tgtggcgatc tttgtggatt gcaggtacaa caatatgaga aggtttatcg
     5101 cccacaattt gcaataaata ttcgcccaaa tcggtttcaa ccactttaat gccttctgct
     5161 tctaaaacgt ggttcatccc aatctcttcg gttaccatcg acttcgattt tacgattttc
     5221 ttcgcatttt tagctttcgc cacgtttttg atgtaatcag tggcttcttc ggcggtttcg
     5281 gcaaagaaaa cgtgtccgcc gttttgggtg actttttcgc ttaattggta gagataagcg
     5341 tctaaattgg ctaaaacgtg gttacgaatt gctttcgcat catcacgcca ctcttcccag
     5401 ttgcccagct catccaccat acgctgacgg tttgcaccga ttgtttcctg agccttaact
     5461 aaggctttac gcacaatctc gttattaact tgctgctcaa cacgttgttt aaacggcagg
     5521 gtgttagttt gtaaataaga catcttattt tcctccctgc attaacactt cggcaatgtg
     5581 catcactttg attggcttac cttcacggct taaacgaccg ccaatgttca tcaagcagct
     5641 tacatctgcc ccgattaaat aatccggctc atcggtatct acatttgcga ctttctcttt
     5701 taccatctcg ccggaaattt ctgccatttt gaccgagaat gtgccaccaa aaccacagca
     5761 agtttcttgg tttttgattg gtaataactc taacccttta acgtgctgta ataaagcaat
     5821 aggctcatct ttaatcccca gcttacggaa taagctgcaa gacgggtgat aaactgcttt
     5881 gcccggtaaa taagccccaa catctttcac gcccagtttg ttcacaataa aatcggttaa
     5941 atcatagaaa cgatcagcca ctttcttcgc acgttctgcc cattcttttt cgccaaagcg
     6001 ttcaaaatga tccggatagg ttttgatcgc atatacacaa gaacctgccg gggcgacaat
     6061 cgggtaatca ttagcttcaa aggtttcaac caagtttttc atacctgcta aagcttgctt
     6121 agtgtaaccg ctgttgatcg caggttgtcc acagcaacct tgtttttcta agaaagtgat
     6181 tttacaacct aatttttcca ataaaagcac ggtatttttt gcaacccctg ctttcattac
     6241 atctgcaatg caggttacgt aaaaattaac gttcataaag aactcccaca gtaaatataa
     6301 ataagaatta aactcaaaaa atgccgttct aagacggcga aaggaagagg tggatttgca
     6361 aaaaatttgc taaatctcac cgcttccttt ctaaatcaag taagctgatt attaacttat
     6421 tataggttat agaacatagg tactataaac aatgccgaaa tcgcagcaat aacgccatac
     6481 acgaacattg gcacggctgt tttcttaata atattgcctt cttgtttctc aatgccggta
     6541 acagaacaca cagccacgat gttgttaata cacaccatat tccccattgc accaccaact
     6601 gattgcaatg caagaattaa ggtaacagat aacccgactt gctgtgccgt tgcatattgt
     6661 actgttccga aggttaagtt agataccgta tttgaccctg agaagaacgc accaattgca
     6721 cctaagaacg aggcaaataa tgtccaagat tgaccggtaa attctgcgaa gtttttacca
     6781 atgatctgaa ccattgagtc tacaccacca attaacatta attttaccat cacaatggca
     6841 cctaaaagtg ctaagaatgg ttttttcact tgtttaaatg aagtgccaaa aatctcttga
     6901 gtatttttac ctgataccga gaaaatcggt aagcaaatta atacggtcac aacaaatgga
     6961 atcaacgcag gaacatacag taatttatag ctagttgcta cattcgtacc taaaatattt
     7021 ttcagtgcaa aaattaagcc ttggctaatt tcaaagttag cgaaaccaag cgaactgaca
     7081 atccaagctg ttgcatcatt tagcattgct tttaatggaa gttgctgaat acgggtgatt
     7141 accaaaatga agatcagtaa ccctgttggt aataatgctt ttgccacttc accaaaggtc
     7201 gctttatcgc cgtcaaggtg gttctcgctt ttcgctaaac caatacccat atttgctacc
     7261 cagactgaca ccaataaacc aatcgccccg gcaactaatg atgggaattc gtagttaaat
     7321 tgagcaataa tgaagtaagg aatgacacag gcaaaaatgc tgatataaat aaacacgata
     7381 ttttgacgaa tttctttcca gctcacaatt aaacgcaatg ccataactgg aataataaac
     7441 gcagcaatag agtggatcag tgcagtaata gaaccaattt caagcacttg tgcatcggtt
     7501 agtaaaccat cttttaataa tggtcccata ccaaaccaag ttggtgtgcc aaccgcacca
     7561 aatgacaccg gcacagagtt cattactaag gctaacattg ccacttgtaa cggtttaaag
     7621 cctaaaccaa ccagaattgg agctgcaatt gccgccggcg taccgaaacc tgatgcgcct
     7681 tcaatcataa atgcaaatgc ccaaccgata atcatcaact gtgccaccgg attcgggtta
     7741 attgtcccta accatttacg catagtgttg gttgcacctg aaacttcaga gaaacggtta
     7801 aataaaatcg ccccgaaaat aacggtaatt ggggttaata catcacctaa accggcaata
     7861 atattggcaa agattaacgt ggtatcatta ccaaaatagg taagctgaag cacaaaaatt
     7921 aataacgccg taagtggtaa agccacataa gacggtaacg cgttacgttt taccattaaa
     7981 taaatgagca aaacaattgg aaatacgctc aggaatagag ccatactgca cctctttata
     8041 tgattataaa atgacctaac tggtcggata ggtgcgaatt gtactcttaa aaaaaaacta
     8101 atgtaactag gttgttacaa ttttgtgatc taactcgcat ttttaatgat tgtatggtta
     8161 aaaaagagcc atatttattt tcatttaata cttaatagtt agccctattt aagacatttt
     8221 taccgaatcg agcaatatgc aattaattaa caaaaaaagg ccgcttgcag atcacagatc
     8281 aacaagcggt cttttttagg caaaaatttg caaattaaac attgaaacgg aagtgcatta
     8341 catcgccgtc ttgaacaata taatctttac cttccaaacg ccatttaccc gcttcttttg
     8401 caccggcttc acctttattg tctatgaagt cttggtaagc aattacttcc gcacgaataa
     8461 agcctttctc aaagtcagtg tgaatcacgg ctgctgcttt tggagcggtt gcacctaccg
     8521 aaaccgtcca cgcacgcact tcttttaccc ctgcggtaaa gtaggtttgt aagttaagta
     8581 aacggtagcc tgcacgaata acacggttta agcccggctc ttcaatgcct aaatcttgta
     8641 gaaattccac tttttcttcg tcatcaagct cggcaatttc cgattcaatc gctgcacaca
     8701 ccggtacaac caccgcccct tctttttcgg caatttcacg cacacggtca agataagggt
     8761 tattttcaaa accgtcttcg ttcacgttgg caatatacat tgtcggtttt aaagttaaga
     8821 agttgtagcc cttaatagcg tgcagttcat ccttatctaa atctaccgaa cgaatcatac
     8881 cggcattttc aagcacaggt aggatttttt ccataatcga taattcaaat tttgcgtctt
     8941 tatcgccacc tttcgcacgt ttttgtaaac gctgaatggc acgctcgcaa ctgtctaaat
     9001 cggcaagtgc caattcggta ttgatgattt caatatcatc agctgggtca attttccccg
     9061 caacgtgaac gatgtcatca ttttcaaaac aacgcacaac gtggccaatc gcatcagttt
     9121 cacgaatatt ggctaagaat ttattaccta aaccttcgcc tttgcttgca cctgccacta
     9181 agccggcaat atccacaaat tccattgtag ttggtaacac tcgctcaggt ttcacaattt
     9241 ccgccaacgc atctaagcga gggtcaggca ttggtaccac accggtattt ggctcaatcg
     9301 tacagaacgg atagtttgct gcctcaatac ctgctttagt taatgcattg aaaagggtcg
     9361 atttaccgac atttggtaaa cccacaatac cacatttaaa tcccattttt tgttccttag
     9421 aattcccctc tccccttgcg ggagagggat aaatttgagc ttcgttcaga agcgaaaatg
     9481 agggagaggg cgaaattgca ataatttcct caaatatgac cgct
//
LOCUS       DANKBV010000084         9475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.084, whole genome shotgun sequence.
ACCESSION   DANKBV010000084 DANKBV010000000
VERSION     DANKBV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9475
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.084"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..937)
                     /locus_tag="PWY43_001673"
     CDS             complement(<1..937)
                     /locus_tag="PWY43_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136538.1"
                     /translation="MHPKFKLTLAALAVSVVLTGCKSSQGPSVVAKNSDDRVIKLNAE
                     TEDKKEETPAEQPRPAPQKPQVTPPAPLLPTTPKLEEKPNKEMVETTPKPEEKPNEKM
                     VEEGNNGEDEIKVITSITPVNTDSKGSTWRVVPLSQTGGVVNLSTARGDHTDYRLTDE
                     NSPLGSSQEFGKTEYNYPIGFELGKNEQNSPFIKTLNKRGDKYLGQHSGTYKDDKLVG
                     NSQKINYLYVNQPYSSYGALFTDANNSNLFHVQLKTGRDSNGISAESEGSNANSAEYS
                     VYTLQGKTGKWNNEVLGDAEYKGNLIARIEKQMDGK"
     gene            1089..3470
                     /locus_tag="PWY43_001674"
     CDS             1089..3470
                     /locus_tag="PWY43_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL2136539.1"
                     /translation="MIVFNKKLLALLISAQFSPLVWAESSDDVAVLNEVSVVGSTPSV
                     AKGSEVTLMKTSDKIIEGKEFKKRSATLGNALAAELGVHSNPFGGGASKPIIRGQEGA
                     RIRILQNGSDVIDMSNLSPDHAVVADSLLADQVEILRGSSTLLYASSSPVGIVNVVDK
                     RIPTAIPEKGYEVELNSRFDTAAKEKVGALGATFGIGNHIAVRAEGLTRHSDNYRVPG
                     INLGERLNYVPDTYNKSKVGTLGLSFVGERGYIGASYSKRRDNYGLPGHNHKFDFCTG
                     HIYGNKRDKYAYTYLYPHLIGEENIGSNPHFHCGTNHAEDGTHSHDNPFGHAHDHTHK
                     GPWVDLESKRIDVKAELRQPFKGVDKIKASYADADYYHDEKDAGVLATRYHKQLKKDQ
                     DYGKPVNIFKNRGKNTRLEVYHAPLGGLTGVWGVQYQTQKSSMNAPKDREVKFPLVEN
                     TNKQFSLFGVEQYMWDSVAVELAGRMEKQKIEIEYDRNEIKRLQEHYRISGGKQVEPD
                     LSPYDETAYAYSGTLNWFFHPDYQLSFTASHNERLPTPMELYYHGQHIATNSFEYGNK
                     DLKKEQSNNVELGLGYQAERIGYKVSVYYNHFKNYIYNENLFRENQLFMRRYNQAKAR
                     FYGVEAEASYRFNDQYQATIFGDMVRGWLTNLPPLKINSDYSVFKDYLPENVEKGKDY
                     LLYRADQNTPRTPPMRLGFRFNAEFTPNWSGDLELIRTFTQRRTSQLEYITEGNTMLN
                     VGLSYSNKWKELDYKISLNGTNLLNQPVYIHTSYHQFVPQMGRNFMLGMEMKF"
     gene            3827..6097
                     /gene="nrdA"
                     /locus_tag="PWY43_001675"
     CDS             3827..6097
                     /gene="nrdA"
                     /locus_tag="PWY43_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL2136540.1"
                     /translation="MNKSLMVTKRNGTREQLNLDKIHRVITWAAEGLENVSVSQVELR
                     SHIQFYEGIRTSDIHETIIKAAADLITKDTPDYQYLAARLAIFHLRKKAYGQFEPPHL
                     YEHVKKLVALGKYDADLLSDYTPEEWETMNGFIDHWRDMTFSYAAVKQLEGKYLVQNR
                     VSGEIYESAQFLYLLVAACLFSKYPTASRLDYIRRFYDATSTFKISLPTPIMAGVRTP
                     TRQFSSCVLIECDDSLDSINATSAAIVKYVSQRAGIGINAGAIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATVYFPIWHLEAESLMVLKNNRGVEENRARHMD
                     YGVQINKMMYQRLIKGGEISLFSPSDVPGLYEAFFADQAKFEQLYLQYEQDPSIRQRK
                     VKAVELFSLLMQERASTGRIYIQNVDHCNTHSPFNPRVAPIRQSNLCLEIALPTKPLA
                     HFYDEQGEIALCTLSAFNLGTLSNLDELEELADLVVRALDALLDYQDYPVLAARNSSL
                     NRRALGVGVINYAYYLAKNQVRYSDGSANNLTHRTFEAIQYYLLKASMNLAKEQGACA
                     YFDQTTYAKGLLPIDTYKKEIDELTQEPLHYDWEQLRQDILQFGLRNSTLTALMPSET
                     SSQISNATNGIEPPRGYISVKASKDGILKQVVPEYERLKAYYELLWDIPNMDGYLHLV
                     GIMQKFVDQAISANTNYDPNRFAEAKVPMKVLLKDLLTAYKYGLKTLYYQNTRDGAED
                     VQDDLDEGCAGGACKI"
     gene            6124..7254
                     /gene="nrdB"
                     /locus_tag="PWY43_001676"
     CDS             6124..7254
                     /gene="nrdB"
                     /locus_tag="PWY43_001676"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL2136541.1"
                     /translation="MAYTTFSQHKNDQLKEPMFFGQNVNVARYDQQKYEIFEKLIEKQ
                     LSFFWRPEEVDVSQDRIDYAQLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISAYYDD
                     LIRDSQLYSLYGEGSYQVDGKTCEVSLRSLKRQLYLCMMSVNALEAIRFYVSFACSFA
                     FAERQLMEGNAKIIKFIARDEALHLTGTQHILNIMTYGNDDPEMAEIARECRQEAYEL
                     FLSAAEQEKAWAEYLFKDGSMIGLNRDILVQYIEYITNIRMQAVGLPLPFKARSNPIP
                     WINAWLVSDNVQVAPQEVEVSSYLVGQIDAKVDSRDFGDFEL"
     gene            complement(7335..8105)
                     /locus_tag="PWY43_001677"
     CDS             complement(7335..8105)
                     /locus_tag="PWY43_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL2136542.1"
                     /translation="MTITNTELEQILNTKLNSSAINDYAPNGLQVEGKREIKKIITGV
                     TATLPLIEKAIERNADAILVHHGYFWKSETPCIRGMKGKRIKQLLVNDINLFGYHLPL
                     DIHPELGNNAQLAKHLGVKNLQGLEDRPNSIPMYGELENPISANELKQKLEKTLQRTV
                     ILCDEFVHSPQKMIKTIGICSGGGQGYIDLAFEKGCDAFISGEISEQTTHSAREQGIY
                     YFACGHHATERDGVKALGEWLAVEYGLDVEFIDIDNPA"
     gene            complement(8188..>9475)
                     /locus_tag="PWY43_001678"
     CDS             complement(8188..>9475)
                     /locus_tag="PWY43_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2136543.1"
                     /translation="VEIKDGQIIRDEHQRADVQETALNKTTTQKTRFWAQLFESFKMA
                     TSAIMAHKMRAILTMLGIIIGITSVISVVALGRGSQQQILSNINNLGTNTMDIMNGTG
                     FGDRRANLTKNLTISDALMLGRQSYVESTTPSSNVSATLIYGNTAVTGSVRGVGEQFI
                     DVKGLKLISGRFFSAEEVKNVAQIIVIDPNTQKDLGLTEPVEGKTILVDKKPLQIIGV
                     AQQSNNMNQSSLTLWAPYTTIMQRISGAKQIDSLTVKIKDDIESQTAEKSITELLVAK
                     HGKKDFFIMNSDTIKQTITSTTNTMTLLISSIALISLIVGGIGVMNIMLVSVTERTRE
                     IGVRMAIGAKQRNILQQFLIEAVVICLIGGVIGILLASAIIWAFNTLGSNFKMMLSPE
                     SVVIAVLCSTLIGVVFGYIPARNASQLNPITALAQE"
BASE COUNT         2701 a         1831 c         2056 g         2887 t
ORIGIN      
        1 tttttccgtc catctgtttt tcaatacgag caattaggtt acctttatac tctgcatcgc
       61 ccagcacttc attattccat ttaccggttt tgccttgcag agtatagacg ctatattcgg
      121 cactattcgc attactgcct tcactctccg ctgaaatacc attgctgtca cgcccggttt
      181 ttagctgaac gtggaacaga ttgctgttgt ttgcatcggt aaagagtgca ccataactgg
      241 aatatggctg attgacgtag agataattga ttttttggct gttgccgacc aatttatcgt
      301 ctttataagt gccggaatgc tgaccgagat acttatcacc ccgtttgttg agtgttttaa
      361 tgaatgggct gttttgctca ttttttccca gctcaaaacc aattgggtag ttatattctg
      421 tcttcccaaa ctcttggctt gagccgagag gggagttttc gtccgttaag cgataatcgg
      481 tgtgatcgcc tcttgcggtg cttaaattca ccacgccacc tgtttggctg agcggtacaa
      541 cacgccacgt agagcctttg ctgtcggtat tcaccggcgt gatagaagta atgaccttaa
      601 tttcatcttc accgttattc ccttcttcta ccattttttc gtttggtttt tcttctggct
      661 taggtgttgt ttctaccatt tctttgtttg gtttttcctc tagcttaggt gttgtaggca
      721 ataacggagc tggtggtgtg acttgtggtt tttggggggc agggcgaggt tgctctgctg
      781 gggtttcttc ttttttatct tccgtttcgg cgttgagttt aattactcgg tcatctgaat
      841 ttttcgctac cacagaagga ccttggctgg atttgcagcc ggttaaaaca acagaaaccg
      901 ctaatgcggc aagggttagt ttgaatttag gatgcataga tattttctcc ttataaaagc
      961 cataaaattt taatggataa atttaccttt tgacaagtag gtaagttaaa aattacataa
     1021 attttttatg gtctattgta aaaaactctt ttttacagta atttattaac cttaaaattt
     1081 ttaaggagat gattgtgttt aataaaaaat tactggcttt gctaatttct gcccaattta
     1141 gcccgcttgt ttgggctgaa agcagcgatg atgttgcagt tttgaatgag gtttctgtgg
     1201 ttggcagtac accgagtgtg gcgaaaggta gcgaagtcac cctaatgaaa accagtgata
     1261 aaatcattga aggtaaagag tttaagaaac gttcggcaac attgggcaat gcgttagccg
     1321 ctgagttagg cgtgcatagt aacccgtttg gtggcggagc gagtaagccg attattcgtg
     1381 ggcaggaagg ggcgagaatt cgcattttgc aaaacggttc agacgtgatt gatatgtcga
     1441 acctatctcc tgatcacgcc gttgttgctg attcactgct tgccgatcag gtcgaaatct
     1501 tgcgtggttc cagcaccttg ctttatgcca gcagttcgcc ggtaggaatt gtaaatgtgg
     1561 tggataaacg tattccgacc gctattcctg aaaagggata tgaggttgaa ctcaatagcc
     1621 gttttgatac cgccgccaaa gagaaagtcg gcgcattagg ggcaaccttt ggcatcggta
     1681 accacattgc ggtgcgagcc gaagggctga cccgccatag tgataactac cgtgtgccgg
     1741 ggatcaattt aggcgagcgc ttaaactatg tgccggacac ttacaataaa tccaaagtcg
     1801 gcacgttggg cttatccttt gtcggtgaac gaggctatat tggtgcctca tacagcaaac
     1861 ggcgggataa ttatggcttg ccggggcata accataaatt tgatttctgt accggtcata
     1921 tctacggtaa taagcgggat aaatacgctt atacctatct ctatccgcat ttgattggtg
     1981 aggaaaatat tgggagcaat ccgcacttcc actgtggaac caaccacgcc gaggacggca
     2041 cacacagcca cgataacccg ttcggacacg cccacgatca cactcacaaa ggcccgtggg
     2101 tggatttaga atcaaaacgg attgatgtga aagccgagct acgccagccg tttaagggcg
     2161 tagataaaat taaggcaagc tatgctgatg ccgattatta ccacgatgaa aaagatgcag
     2221 gcgtgcttgc cacccgttac cataaacagc tgaaaaaaga tcaggattac ggcaagccgg
     2281 tgaatatttt caaaaatcgg ggcaaaaata cccgcttgga ggtctatcac gcaccgctag
     2341 gtggcttaac cggtgtatgg ggtgttcagt atcaaacgca aaaaagcagt atgaatgcgc
     2401 cgaaagatcg cgaagtgaaa ttcccgctgg ttgaaaatac caataaacag ttcagcctgt
     2461 ttggggttga gcagtatatg tgggatagcg ttgctgttga gcttgccgga cgaatggaaa
     2521 agcagaagat tgagattgag tatgatcgca atgaaatcaa acggttacaa gaacactacc
     2581 gtatcagtgg cggcaaacag gttgagccag atctttcacc ctatgatgaa acggcttatg
     2641 cctattccgg cacgcttaac tggttcttcc accctgacta tcagctttcg tttactgcct
     2701 cgcacaatga acgcttgcca acgccaatgg agctgtatta tcacgggcag catattgcta
     2761 ctaattcgtt tgaatacggc aataaagact tgaaaaaaga gcagtcaaat aatgtggagt
     2821 tgggcttggg ctatcaagct gaacgtattg gctataaagt gagtgtttat tacaatcact
     2881 ttaaaaacta catctacaat gaaaatcttt tccgtgaaaa tcagctattt atgcgccgtt
     2941 ataatcaggc aaaagcccgt ttttacgggg tagaagcaga ggcaagctac cgctttaacg
     3001 accaatacca agccacgatt ttcggcgata tggtacgagg ctggctaaca aatttaccac
     3061 cattgaagat aaatagcgat tatagtgtgt tcaaagatta tctgccggaa aatgtggaaa
     3121 aagggaaaga ttatcttcta tatcgggctg accaaaacac ccctcgtaca ccgccaatgc
     3181 gtttaggttt tcgctttaat gcggaattta ccccaaattg gtcgggggat ttagagctga
     3241 tccgcacctt tacccaacgc cgtacgagcc agttggaata tattaccgaa ggtaatacga
     3301 tgttgaatgt cgggttgtcg tatagcaata agtggaaaga gctggattac aaaatcagtc
     3361 taaacggcac aaacttactg aatcagccgg tgtatatcca cacttcgtat caccaatttg
     3421 taccacaaat gggacgaaat tttatgttgg gaatggagat gaaattttaa gttctcgaca
     3481 taacaagaca agcggttatt ttttcttcaa attttgcaaa aaaacggcta aaaataaccg
     3541 cttgtttaaa actcaggtat tggtaatttg gataaaatag tgctgttttt cctttcttgt
     3601 tattttcgga gcttaccaaa atttcctgtt tgttgttaaa taagacaatt aactttatcc
     3661 ctttattaaa aatatggtta tttataaata acttagcatc tgtcaagaga aaaagtaaaa
     3721 atttattggt tgatgttttg ctttaagcac actatcttgt gtttacttta atgaaaaaca
     3781 caagatgtag tgttttacta aggtatttta taaagggtta ataattatga ataaaagttt
     3841 aatggtaaca aagcgtaatg gcacgcgcga gcaactcaat ctagataaaa ttcatcgtgt
     3901 gattacttgg gcggcagaag ggttagagaa tgtttctgtt tcacaagtag aactccgttc
     3961 tcacattcaa ttttatgaag gtattcgcac ttcagatatt catgaaacta ttatcaaggc
     4021 tgcagctgat ctaatcacta aggatacgcc agattatcaa tatttagccg cacgcttggc
     4081 gattttccac ttacgcaaaa aagcttatgg acaatttgag ccaccgcatt tatatgagca
     4141 cgttaaaaaa ttagttgcgc taggtaaata tgatgcagac ttgctgagcg actatacgcc
     4201 cgaagaatgg gagacaatga atggatttat tgatcactgg cgggatatga cgttttccta
     4261 tgcagcagta aagcagttgg aaggaaaata tttagtgcag aatcgtgtta gcggagagat
     4321 ttatgaatcg gctcaattct tgtatttact cgtagctgcc tgtttatttt ctaagtatcc
     4381 aacagcaagc cgcttagatt atattcgccg tttttatgat gcgacttcca ctttcaaaat
     4441 ttctttgcca acaccgatta tggcaggagt gcgtacacct actcgtcaat ttagttcctg
     4501 tgtgttaatt gagtgtgatg atagcttaga ttccatcaat gcaacctcgg ctgcaatcgt
     4561 taaatatgtt tcacagcgtg ctgggatcgg tattaatgcc ggcgcaattc gggcattagg
     4621 tagccccatt cgtggtggag aagcatttca taccggctgt attccctttt ataaacattt
     4681 tcaaactgca gtaaagtctt gctctcaagg tggtgttcgc ggtggagcgg ctaccgtgta
     4741 ttttccgatt tggcatttgg aagcagaaag cttaatggta ttaaaaaata accgtggtgt
     4801 ggaagaaaac cgtgctcgcc atatggatta cggggtacag attaataaaa tgatgtatca
     4861 acgtttaatt aaaggcggtg agattagttt atttagtcca tcagatgtgc cggggttata
     4921 tgaagcgttt tttgctgatc aagctaaatt tgagcaactt tatttgcaat atgagcaaga
     4981 tccgagcatt cggcaacgta aagttaaagc tgtagaattg ttttcattac tgatgcaaga
     5041 gcgggcttca acagggcgaa tttatattca gaatgtcgat cattgtaata ctcatagccc
     5101 atttaatcct cgtgtcgcac cgatacgcca atcgaacctc tgcttagaaa ttgctttgcc
     5161 aactaagcca ctggcacatt tttatgatga acaaggggaa atcgcattat gtacattatc
     5221 ggcatttaat ttaggcacac tcagcaattt agatgagtta gaagaattgg ctgatttagt
     5281 ggttcgtgcg ctagatgccc tattagatta tcaagattat ccggtattag ctgcacgtaa
     5341 cagctcatta aatcgccgcg cattaggtgt aggcgtgatt aactatgctt actatttagc
     5401 taaaaaccaa gtgcgttatt ccgatggcag tgctaataat ttaactcacc gtacctttga
     5461 agcgattcag tattatttat taaaagcatc gatgaattta gccaaagaac aaggggcgtg
     5521 tgcttatttt gatcaaacca cttatgccaa agggttactc cctatcgata cttataaaaa
     5581 agaaattgat gaattaactc aagaaccgct tcattatgat tgggaacaac tacgtcagga
     5641 tattctgcaa tttggcttgc gcaattccac tttaactgcc ttaatgccat cggagacttc
     5701 gtcgcaaatt tcgaatgcca ccaatggaat tgaacctccg cgtggctata ttagtgttaa
     5761 agcatcaaaa gacggtattt taaagcaggt tgtgccggag tatgaacgtt taaaagcata
     5821 ctatgaactg ctttgggata ttcctaatat ggacggctat ttgcatttgg ttgggattat
     5881 gcagaaattt gttgaccaag cgatttcggc gaatacaaat tacgacccaa accgctttgc
     5941 agaagctaaa gtaccgatga aagtattatt aaaagatcta ttaacggcgt ataaatacgg
     6001 gttaaaaacc ctttactatc aaaatacccg tgatggagca gaagatgtgc aagacgactt
     6061 agatgaaggc tgtgctggtg gtgcttgtaa aatttaataa gaacagaaat aaaggagaat
     6121 aacatggcat atactacgtt ttcacaacat aaaaatgatc aactaaaaga acccatgttt
     6181 tttggacaaa atgtgaatgt ggcgcgttat gatcaacaaa aatacgaaat ttttgaaaaa
     6241 ctgattgaaa aacaactctc atttttctgg cgaccggaag aagtggatgt gtcgcaagac
     6301 cgcattgact atgcccaact gcctgaacat gaaaagcaca tttttatcag caatttaaaa
     6361 taccaaacct tgttggattc cattcaaggg cgtagcccga atgtggcgct tttgccatta
     6421 gtgtctatcc ccgagcttga aacctggatt gaaacttgga catttagcga aacaattcac
     6481 agtcgttctt acacacatat catccgtaat attgtcaatg atccatcaat agtatttgat
     6541 gatattgtca caaacgaaga aattattaag cgtgccaaag atatttccgc ctattatgat
     6601 gatttgattc gtgacagcca actctatagt ttgtatggtg aaggcagtta tcaggttgat
     6661 ggaaaaacct gtgaggtcag tttgcgtagt ctaaaaagac aactttatct ctgtatgatg
     6721 agtgtgaatg cgcttgaagc aattcgtttt tatgtctctt ttgcttgctc atttgccttt
     6781 gcggaacggc agcttatgga aggaaatgcc aaaatcatca aatttattgc acgtgatgaa
     6841 gcattacatc ttaccggcac tcagcatata ttgaatatta tgacctatgg caatgatgat
     6901 cctgaaatgg cagaaattgc acgtgaatgt cgccaagagg cctacgagct ttttttaagt
     6961 gcagctgaac aagaaaaagc gtgggcggaa tacctgttta aagacggttc gatgataggg
     7021 ctaaatcggg atattttggt gcaatatatt gaatatatca ctaatattcg gatgcaggct
     7081 gttggactac cattaccatt taaggcacgt tccaacccca ttccgtggat caatgcgtgg
     7141 ttggtttccg ataatgtaca ggtagcacca caagaagtgg aagtcagttc ttatttggtt
     7201 ggccagattg atgctaaagt agatagccgt gattttggcg attttgaact ttagactatg
     7261 atatacaagc ggttgatttt tagtgatttt ttgcaaattg caaaattttc aggaaattca
     7321 accgcttgta ttttttatgc agggttatca atatcaataa attcaacatc taaaccatat
     7381 tcaacggcta accattcacc taaggctttt acgccatctc gttccgtggc gtgatgacca
     7441 caagcaaaat aatagattcc ttgttcacgg gcagagtggg tggtctgttc ggaaatttcg
     7501 ccgctaataa aggcatcaca gcctttttca aaggctaaat caatatagcc ttgtcctccg
     7561 ccggagcaaa tcccgattgt tttaatcatt ttttgtgggc tatgaacaaa ttcatcacat
     7621 aaaataacgg tgcgttgtaa cgttttttca agtttttgtt ttagttcatt ggcggaaatc
     7681 ggattttcta attcaccgta cattggaatt gaattaggtc tgtcttctaa accttgtaga
     7741 ttttttacgc ctaaatgctt agcaagctgg gcgttgttac ctagttcggg gtgaatatcc
     7801 agtggtaggt gataaccaaa gagattgatg tcattcacta aaagctgttt aatgcgttta
     7861 cctttcattc ctcgaatgca tggggtttcg cttttccaaa aataaccgtg gtggactaaa
     7921 attgcatcgg cgtttctctc aatcgctttt tcaatcaggg gaagtgtggc agtcacaccg
     7981 gtaatgattt ttttgatttc tcttttccct tcaacttgca agccatttgg ggcatagtcg
     8041 ttaatggcgg agctgttaag tttggtgttc agtatttgtt ctagttcagt attggttatt
     8101 gtcattttat ctccttaaac ttggcataat aatggatgta agggcaaaaa attttttgcc
     8161 ctacagtgta gcagatagtt gtcgcattta ctcttgtgcc agagcagtaa ttgggtttaa
     8221 ttgggaggcg tttcttgccg gaatgtagcc aaataccaca ccgataagag tagagcagag
     8281 tacggcaatc acaaccgatt caggtgaaag catcatttta aaattcgagc ctaaggtatt
     8341 aaatgcccaa ataatagcac ttgcgagcaa aatgcctatt acaccaccaa tcaggcaaat
     8401 aaccaccgct tcaattaaaa attgttgcaa aatattgcgt tgttttgcac caatcgccat
     8461 tcggacacca atttctcggg ttcgctcggt gacggaaacc aacataatat tcattacccc
     8521 gataccgccg acaatcagcg aaataagggc gatagaggaa atcagcaagg tcattgtatt
     8581 ggtggtactg gtaatggttt gtttgatcgt atcactattc ataataaaga aatctttttt
     8641 gccgtgttta gctaccaaca attcggtaat gcttttttcg gcagtttggc tttcaatatc
     8701 atctttaatt ttaaccgtga gtgaatctat ctgttttgca cctgaaatgc gttgcataat
     8761 agtggtataa ggcgcccaca gggtaaggct actttggttc atattatttg actgttgtgc
     8821 cacaccaata atctgtaacg gttttttatc cactagaatc gttttacctt caacaggctc
     8881 tgtcagcccc aaatcttttt gagtgttagg gtcaattaca ataatctgtg ccacattttt
     8941 tacttcctcg gcactgaaaa aacgccctga aatcagcttt aaccctttca catcaataaa
     9001 ttgctctcca acacctcgta ctgaaccggt tactgcggta ttaccataaa tcaatgtggc
     9061 actgacattg cttgaaggag tagtgctttc cacatagctt tgccgcccca acattaaggc
     9121 atcactgatg gttaaattct tagtcagatt ggctcttcta tcgccaaagc cggttccatt
     9181 cataatatcc atcgtatttg tgcctaggtt gttgatatta gaaaggattt gttgttgcga
     9241 gcctcgaccg agagcgacta cggaaatcac agaggtaatg ccaataataa tgcccagcat
     9301 cgtgaggatt gcccgcattt tatgtgccat aattgcactg gttgccattt tgaaggattc
     9361 aaacagttga gcccaaaagc gagttttttg tgttgtcgtt ttattgagag ctgtttcctg
     9421 aacatcagca cgttggtgtt catctcgaat gatttgtcca tctttaattt caact
//
LOCUS       DANKBV010000085         9368 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.085, whole genome shotgun sequence.
ACCESSION   DANKBV010000085 DANKBV010000000
VERSION     DANKBV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.085"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            38..838
                     /locus_tag="PWY43_001679"
     CDS             38..838
                     /locus_tag="PWY43_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDL2136544.1"
                     /translation="MCQLLGMNCNTPTDITFSFDGFRRRAGLTDHHTDGFGIAFFEGK
                     GVRVFRDNRPGASSPMAEAISQYQIKSLNVVAHIRKATRGDVNLENTHPFIRELWGEN
                     WVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCRIVSELKEMYPAKPSEKEIFDAVVQ
                     ITSRIAEHGVFNFVLSNGHWMIARCTTNLHYVCRKAPFGKALRDDDVSIDFSQYTKIT
                     DKVTIITTQPLTKNENWTKMKNGGYVFFKDGDVVYEIEGTLPEAKPHV"
     gene            complement(872..1765)
                     /gene="ispA"
                     /locus_tag="PWY43_001680"
     CDS             complement(872..1765)
                     /gene="ispA"
                     /locus_tag="PWY43_001680"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDL2136545.1"
                     /translation="MSYLLATDLTHLQQRINHFLAEKLKTTDSVGSPLVEAMAYAVLL
                     GGKRVRPFLIYATGRMLGVPLEKLDHSAAAMEAIHAYSLVHDDLPAMDDDRLRRGKPT
                     CHIAFDEATAILAGDALQSFAFELLATDPHLTDREKVAQVTELATASGAKGMCLGQSL
                     DLIAENKAVSLEELELIHRNKTGALILSSVMMGLNLSEHSQNLAIKQPLVNYAKAIGL
                     AFQVQDDILDVIGDTDKIGKTVGSDEHLNKSTYPKLLGLDGAKQKAEMLYQTALHSLE
                     QLPFDTTALKELANFIVKREN"
     gene            complement(1771..2001)
                     /gene="xseB"
                     /locus_tag="PWY43_001681"
     CDS             complement(1771..2001)
                     /gene="xseB"
                     /locus_tag="PWY43_001681"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL2136546.1"
                     /translation="MAKKPTQDFESTLKELEAIVTHLESGDLPLEDALNEFETAIKLV
                     QQGQERLQKAEQRIQILLNKNDTAPLSDYERE"
     gene            2126..2914
                     /locus_tag="PWY43_001682"
     CDS             2126..2914
                     /locus_tag="PWY43_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDL2136547.1"
                     /translation="MQVTELNIYPIKSTQAIQVSQAFVEPKGLNFDREFMLAEPNGKF
                     ITARKDPELYRLAAFPIATGVMITHTSGQKCVALYQDFVEEQSSEVWGTHFNAKVAAE
                     AVNQWLSEIFGRAVQLRWLGSQSQRIVANFADHPMSFADSNPVSLMSLKSLEQVQQWS
                     PIPLEMARFRPNIVIDGNVAFEEEQWQQVQIGEVLFTKAALCTRCILITRDLNTLELD
                     PKSEPFRTLKEKHCDEKGKPVFGIHLVPQNSGVIKVGDPVIIQL"
     gene            2946..4892
                     /locus_tag="PWY43_001683"
     CDS             2946..4892
                     /locus_tag="PWY43_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2136548.1"
                     /translation="MYQRSQSRTVLFPILLFVGINLVILTLSRLVLGVWQSDRVSAVD
                     GWIPLILQGIRIDISSLCWLFGLPALFSVLCFHKNVLGSIWQTVLRIWLTVGSVFILF
                     MEVATPNFIETFDLRPNRLFIEYLVHPREVFTMLINGHLWAVICGIGIAMISLIIYWK
                     LSGWATKNLHFPSWKMRPLLALLVAAIVFVGGRSSFAHRGINPAMVAFSSDPLVNSLV
                     LNSGYSVLYAVQQLKDEDKSSEQYGKMSVEEMFNIVKQVRNRPASDYISDEIPTLTQN
                     KATYQGKPKNIVIILEESLGAQFVGSLGGKNLTPNLDKLSEQGWYFTNLYATGTRSVR
                     GIEAVTAGFTPTPARSVVKLTKSQTNFFSIAELLKRQGYHTSFIYGGEKHFDNMASFF
                     YGNGFQQIIDEKDYKNPKFTATWGVSDEDLFDKANGTFNQLRKSGKPFFSLVFSSSNH
                     DPFEFPDGKIELYEQPKQTRHNAAKYADYAIGHFFELAKKSEYWQDTVFLVIADHDSR
                     AVGEHLVPIQHFHIPALLLGEHIQPRTDNRLVSQLDMPTTLLSVAGISGQYPMIGYDL
                     TQDDPNRAFMQYDATQAMMRGNDVVVMRPNLPIEGFSYDKANYKLTPKEQPESFKKEA
                     LAHALLGSYLYKNQLYKLPEETKQ"
     gene            complement(4963..5880)
                     /gene="nagK"
                     /locus_tag="PWY43_001684"
     CDS             complement(4963..5880)
                     /gene="nagK"
                     /locus_tag="PWY43_001684"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDL2136549.1"
                     /translation="MAIYYGLDVGGTKIELAAFNEKLEKLHSERVPTPQTNYQDWLTA
                     IKTLVETADTKFDEKGTVGLGIPGFVNRETGIAEITNIRVADGKTILKDLAECLGREV
                     RAENDANCFALSEAWDESNKPYSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
                     QLNYHALKLLDWDKAPIYACGCGNQACLDTYISGRGFELLFNDLVGEKISAKAIIERF
                     YQQETKVVEFVEKYIELMAISISNLITILDPDMIVFGGGLSNFDYIYQALPKALPKHL
                     MRSAKVPVIKKAIHGDSGGTRGAAALFLK"
     gene            complement(5893..6255)
                     /locus_tag="PWY43_001685"
     CDS             complement(5893..6255)
                     /locus_tag="PWY43_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136550.1"
                     /translation="MYQVFIAEPKDPNLEKGLLKHFIKSYLTQEELDFLHKILTNLPN
                     IRPFWGDTGYKYIVIDNVYFLYEVSESEKKVILLGTKFNNKRKTFNFANTKTQKKSMK
                     KWINDLAIYKPHKINQSQ"
     gene            complement(6263..6424)
                     /locus_tag="PWY43_001686"
     CDS             complement(6263..6424)
                     /locus_tag="PWY43_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136551.1"
                     /translation="MEMSIEKIAELLSDKEKDDYPFSIEERQFAAQASADTWEAILNN
                     TIQPFDFKK"
     gene            complement(6493..7215)
                     /locus_tag="PWY43_001687"
     CDS             complement(6493..7215)
                     /locus_tag="PWY43_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2136552.1"
                     /translation="MFKKFSLYTLAMLLIPVVTWLANWHWSLAIQYAEFDFDYFLYLI
                     TETGSVPYAIITCVVFALLLAWVVHKRYSLIFVITVCVASMVATQAIKTAMKSTFQEP
                     RPFVTALFQDKTDDFYQLTREQRGLAVMEKIGANQNFVTAHQAKEVGYSFPSGHTIFA
                     VSWLLLVVGFCRNMRGQAVVLAHFFAISWAALMLISRLRLGMHYPIDLFTSSLIAFLI
                     NLALFVWFVPWLESKKWFAKKP"
     gene            complement(7276..7590)
                     /gene="yhbY"
                     /locus_tag="PWY43_001688"
     CDS             complement(7276..7590)
                     /gene="yhbY"
                     /locus_tag="PWY43_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL2136553.1"
                     /translation="MSITLTTKQKQFLKGLAHHLSPVVMLGGNGLTEGVLAEIDNALD
                     HHELIKVKVAGADRETKQLIIDAILRETKAAEVQTIGHILVLYRQSEEKKISLPKATK
                     AI"
     gene            7717..8475
                     /locus_tag="PWY43_001689"
     CDS             7717..8475
                     /locus_tag="PWY43_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2136554.1"
                     /translation="MPTLSVAMIVKNEAQDLRTCLDTVKDWVDEIVILDSGSTDETPQ
                     IAAEYNARFYTNTDWQGFGKQRQLAQQYVTSDYVLWLDADERVTAELKQSILQAVKND
                     EKNTVYKISRLSEVFGKQIRHSGWYPDCVVRLYRTEFAKYGDELVHEKVHYPKEAKIQ
                     KLQGDLLHFTYKDIHHYLVKSASYAKAWAIQRANAGKTASLFDGVTHAIGCFVKMYLL
                     KAGFLDGKQGFLLAVLSAHSTFVKYADLWNRTRK"
     gene            complement(8540..>9368)
                     /locus_tag="PWY43_001690"
     CDS             complement(8540..>9368)
                     /locus_tag="PWY43_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL2136555.1"
                     /translation="LQAVKNEQFDAVICFVSDWHNAKLTWQSGIKYRLAPATKLFQFL
                     YNHRLTQRRSQSAKAESEYNLDLARAFLSKHNIPIVEPHTPYLSVEQQTVQAQKAKLC
                     EQLNITTVKKWVFVHSSTGGSANNLSIEQYAELIQGILAEFDCYVVLTAGKGESEKAQ
                     QLAQQINHNNVVVYDKNEGLQDFTHSLACADLFIAGSTGPLHISGALNIPTIGFYPSR
                     LSAIPRRWKPINDANKHIAFCPPSDKASQMNLTLISISEALKTIVPFIRRMWNTPSL"
BASE COUNT         2714 a         1831 c         1970 g         2853 t
ORIGIN      
        1 tttttgttgt aaattattta ttttaaagaa gtcaattatg tgtcaattac ttggaatgaa
       61 ttgtaatacg ccaaccgata ttacattttc ttttgatggt ttccgccgta gagcagggct
      121 tacagatcat catacagacg gattcggtat tgcctttttt gagggtaaag gcgttcgggt
      181 ttttcgtgat aatcgcccgg gggcatcatc gccgatggca gaagcgatca gccaatatca
      241 aattaaatca ctcaatgtgg ttgcacacat tcgtaaagct acccgtggcg atgtgaattt
      301 ggaaaatacc cacccattca ttcgtgaatt atggggcgag aactgggtgt ttgcccataa
      361 cggcacagtg gaaggtgttg gggtatgcga ggagtgccac taccagccga ttggtacaac
      421 cgattccgaa gcggcatttt gccgtattgt gtcggaatta aaagagatgt atccggctaa
      481 gccaagcgaa aaagagattt ttgatgctgt ggtgcaaatt acctcaagaa ttgcggaaca
      541 cggggtattt aattttgttt tatccaacgg tcattggatg attgcccgtt gtacaactaa
      601 tctgcattac gtttgccgta aagcaccgtt tggcaaagcc ttgcgtgatg atgatgtgtc
      661 gattgatttt agtcaatata ccaaaattac tgataaagtg acgattatta ccactcagcc
      721 acttaccaaa aatgaaaact ggaccaaaat gaaaaatggt ggctatgttt tctttaaaga
      781 cggcgatgtg gtatatgaaa ttgaaggcac attgccggaa gccaagcctc acgtttaatc
      841 actaaaaacc tagcttcggc taggtttttt cttagttttc acgcttaaca ataaaattag
      901 ctaactcttt taatgcggtg gtatcgaaag gcaactgttc cagcgaatgg agagcggttt
      961 gataaagcat ttcggctttt tgttttgccc cgtctaagcc caacaatttc gggtatgtgc
     1021 ttttatttaa gtgttcatct gaacccactg ttttgccgat tttatcggta tcaccaatga
     1081 catctaaaat atcatcttgc acctgaaagg ctaagccaat cgctttggca tagtttacaa
     1141 gcggttgttt tatggctaaa ttttgcgaat gttcggaaag gttcaatccc atcatcacgg
     1201 atgacagtat caatgccccg gttttgttgc gatgaattaa ttcaagttct tccaaactga
     1261 cagctttatt ttccgcaatc aaatccaaac tttgccctaa gcacattcct tttgcaccgg
     1321 aagcagttgc caattctgta acttgagcga ctttttctcg gtcggtcaaa tgcggatcgg
     1381 ttgccagcag ttcaaacgca aaactttgta acgcatcgcc ggctaaaata gcggtagctt
     1441 catcaaaggc aatatggcag gttggcttgc cacggcgtaa tctatcatcg tccattgccg
     1501 gtaaatcatc gtgaaccagc gagtaagcgt gaatagcctc cattgccgcc gcactatgat
     1561 ctaatttttc caacggcaca ccaagcattc tgccggtggc atagattaaa aacggtcgca
     1621 cccgtttacc gcccagcaat accgcataag ccattgcttc tactaaaggc gaaccaacgc
     1681 tatccgttgt ttttaatttt tcagcgagaa aatggttaat ccgttgttgc aggtgagtca
     1741 gatcggttgc taatagatag ctcattgtga ttattctctt tcgtaatcgc taagtggggc
     1801 agtatcgttt ttatttaata agatttgaat acgttgctcg gctttttgta accgttcttg
     1861 cccttgttgc actaatttga ttgcagtttc aaattcgttg agggcatctt caagcggtaa
     1921 atcaccggat tccaaatggg taacgattgc ttctaattct ttaagtgtgc tttcaaaatc
     1981 ttgagtgggt ttctttgcca ttgttgctga cctattgctg taaaagttgc tcaagtttac
     2041 cgttctcaca agggatttgc aaattccttt tacttttcga ccgcttgcag gtattgttat
     2101 cctatttcca aatagaggat tttctatgca agttaccgag ctcaatattt acccgataaa
     2161 atcaactcaa gctattcaag tttcacaggc atttgttgaa ccgaaaggct taaattttga
     2221 ccgtgaattt atgctagccg agcctaacgg caaatttatt actgcccgta aagatccgga
     2281 attataccgt ctagcggctt tcccgattgc gacaggggta atgattaccc atacaagcgg
     2341 tcaaaaatgc gttgcacttt accaagattt tgtggaagag cagagtagtg aagtgtgggg
     2401 aacacatttt aatgccaaag tggcggcaga agcggtcaat caatggctga gtgaaatttt
     2461 tggtagggct gtgcaactac gttggcttgg ctcgcaaagc caacgaattg ttgcgaattt
     2521 tgccgatcac ccaatgagtt ttgcagatag caacccggtg agtttgatgt ctctaaaatc
     2581 gcttgagcaa gtgcaacaat ggtcgccgat tccactcgaa atggctcgtt ttcgcccgaa
     2641 tattgtgatt gatggcaatg tggcatttga ggaagagcag tggcaacaag ttcaaattgg
     2701 cgaagtgcta tttaccaaag cggcattatg tactcgttgc attttgatta cacgggattt
     2761 aaacacgctt gagttagacc ccaaatcgga gccgtttaga accttaaaag aaaagcattg
     2821 cgatgaaaaa ggtaagccgg tgttcggtat tcaccttgtg ccacaaaaca gtggtgtgat
     2881 aaaagtaggc gatcctgtca ttattcaatt ataatatgcc gatatttttt aaaaggagat
     2941 tcaaaatgta ccaacgcagt caatcaagaa ctgtgctatt tccaatccta ctatttgttg
     3001 ggattaattt agttatcctc accttatccc gtttagtgtt aggcgtttgg caatctgatc
     3061 gagtatccgc tgttgatgga tggataccat taatactaca aggtattcgc attgatatat
     3121 cttcgctatg ttggctattt ggcttaccgg cattatttag cgtgttgtgt tttcataaaa
     3181 atgtactggg ttcgatttgg cagactgttt taagaatttg gctaactgtc ggtagtgtat
     3241 ttattctttt tatggaagtc gccacaccga attttattga aacctttgac cttcgcccaa
     3301 atcgtctttt cattgagtat ttggtccatc cgagagaagt gttcacaatg ctgattaacg
     3361 gacatttatg ggctgttatt tgtgggattg gcattgcgat gatttctctc atcatttatt
     3421 ggaaattatc gggttgggca accaaaaatt tgcatttccc gagctggaaa atgcgtccgc
     3481 tactggcttt attggttgct gcaatagttt ttgttggtgg gcgttccagt tttgcccatc
     3541 gagggattaa tccggcaatg gtggcatttt caagcgatcc gcttgtcaat tcactggtgc
     3601 taaattcagg ttattccgtg ctttatgcgg ttcagcaatt gaaagatgaa gataaatctt
     3661 ccgaacagta tggaaaaatg tctgtggagg aaatgttcaa cattgttaag caggttcgta
     3721 atcgaccagc ttcagattat atttcagatg aaattcccac cctcacgcaa aataaagcga
     3781 cttatcaagg aaagcctaaa aacattgtga ttatcttaga agaaagttta ggggctcaat
     3841 ttgtcggttc gctaggaggg aaaaatctca ctccaaattt agataaactt tcagaacaag
     3901 gctggtattt taccaatctt tatgcaacgg gtactcgttc ggtgagagga attgaagcgg
     3961 taacggctgg ttttacacca acaccggcaa gatctgtggt aaaactgacg aaaagccaaa
     4021 ctaacttttt tagtattgcc gaattattaa aacgtcaagg ataccatact tcctttattt
     4081 atggtgggga gaagcatttt gataatatgg cgagcttttt ctacggtaat ggtttccagc
     4141 aaattatcga tgaaaaagat tataaaaacc ctaaatttac cgcaacttgg ggcgtgagtg
     4201 atgaagatct gtttgataag gcaaatggaa cttttaatca actgcgcaaa tcaggtaaac
     4261 cgttttttag tttggtattc agttccagta accacgatcc gtttgaattt ccggacggca
     4321 aaattgaact ctatgaacaa ccgaagcaga ctcgccataa tgcggcaaaa tatgccgatt
     4381 atgctatcgg gcatttcttt gagttagcga aaaaatcaga gtactggcaa gatacggtgt
     4441 ttttagtgat tgcagatcac gattcaagag ccgttggcga gcatttagtg ccaattcaac
     4501 acttccatat tccggcactt ttattaggcg aacacattca gccaagaacg gataaccgtt
     4561 tagttagtca gctcgatatg ccgacaacat tactttctgt ggcgggaata agtggtcaat
     4621 atccgatgat agggtatgat ttaacgcaag atgacccaaa ccgtgccttt atgcaatatg
     4681 atgcgacaca ggcgatgatg cggggaaatg atgtggtggt aatgcgacca aatttaccga
     4741 tagaaggatt tagctacgat aaagctaact ataaactcac ccctaaagaa cagccggaat
     4801 cgtttaaaaa agaggcgtta gcacacgcat tgcttggcag ttatttatat aaaaatcagt
     4861 tatataaatt accggaagaa actaagcagt aaggcaaatt aaaagagagg gctgcaatat
     4921 ttttattaaa aatgaccgct tgccctctct caaatttgtt cattacttta agaataatgc
     4981 tgctgcacca cgagtgccac ctgagtcgcc gtgaattgct tttttgatga ccggcacttt
     5041 cgctgaacgc attaagtgtt ttggtaatgc ttttggtaga gcttggtaga tataatcaaa
     5101 gttggataaa ccgccaccga atacaatcat atccggatct aaaatcgtaa tcagattgct
     5161 gatactgatt gccattagct caatatattt ttcgacaaat tccaccactt tggtttcttg
     5221 ttggtagaag cgttcgataa tcgctttcgc cgagattttt tctcctacta aatcattaaa
     5281 cagtaactca aaaccccgcc ctgaaatata ggtgtctaaa caggcttggt tgccacaacc
     5341 acatgcataa attggggctt tatcccagtc gagcaattta agtgcgtggt agttaagttg
     5401 catatgccca acttcgcctg ccattccgat tctgcctgaa tgaattttac cgtcaaacac
     5461 aaaaccaccg ccaaaaccgg tgccgataat caaccctaat acggtggaat aaggcttatt
     5521 actttcgtcc cacgcttcag acagggcaaa gcagttagca tcgttttcgg cacgcacttc
     5581 acgccctaaa cattcggcta aatctttcag aatcgttttg ccgtcagcca ctcgaatatt
     5641 ggtaatttcc gcaatgccgg tttcacggtt tacaaagccc ggaatgccca gcccgacagt
     5701 gcctttttcg tcaaattttg tatcggcagt ctcaaccagc gttttaattg ccgtcagcca
     5761 atcttgataa tttgtctgcg gtgtcggcac acgttcgctg tgtagttttt ctagtttttc
     5821 attaaaagcg gctaattcaa tttttgtgcc gcccacatct aagccataat aaattgccat
     5881 aattacgctc tcttattgcg attgattgat tttatgcggt ttataaattg ctaaatcatt
     5941 aatccatttc ttcattgatt ttttctgggt tttcgtattc gcaaaattaa aagttttccg
     6001 tttattatta aatttggtgc caagtagaat gactttcttt tcactttcgg aaacttcata
     6061 caagaagtaa acattatcaa tgacgatata tttgtaaccg gtatcgcccc aaaaaggtct
     6121 gatgttgggt aaattagtga gaattttatg caggaaatcg agttcttctt gagtaagata
     6181 agacttaatg aagtgtttca ataagccttt ctctaagttt gggtcttttg gttctgcaat
     6241 aaaaacttga tacatagcaa gcctattttt tgaagtcaaa aggttgaata gtgttattta
     6301 agattgcctc ccaagtatca gcactagcct gggcggcaaa ttgtctttcc tcaatactaa
     6361 acggatagtc atctttttct ttatcgctta atagttcagc tattttttct atcgacattt
     6421 ccattctaag ctccttattt taggtactta ttcaataatt tgtattgtat attgatttgg
     6481 ctaaattgcc aattaaggct ttttagcaaa ccatttttta ctttccaacc aaggcacaaa
     6541 ccacacaaat aaggctaaat tgattagaaa cgcaattaaa ctactggtga ataaatcaat
     6601 cgggtagtgc atacctaaac gcaagcggct aatcagcatt aacgccgccc aactgattgc
     6661 aaaaaaatga gccaaaacga ccgcttgtcc tcgcatatta cggcagaaac ctaccaccaa
     6721 taatagccaa gaaaccgcaa aaatagtgtg gcctgatggg aaagaatagc ccacttcttt
     6781 ggcttggtga gcggtaacaa aattttgatt tgcaccgatt ttttccatta cggcaagccc
     6841 acgttgctcg cgtgttaatt gataaaaatc gtctgtttta tcttggaaaa gagcagtgac
     6901 aaatggacga ggctcttgaa aggttgattt catcgctgtt ttgattgctt gagtggctac
     6961 cattgaagcg acacacacgg taatcacaaa aattaaagag tagcgtttgt gaaccaccca
     7021 cgccagtaat aaggcgaaaa ccacacaggt gatgatggca taaggtacgg agccggtttc
     7081 ggtgattaaa tataaaaagt agtcgaaatc aaattcagcg tattgaattg ccagagacca
     7141 gtgccaattt gccagccaag tcaccacagg aatgagcaac attgcaaggg tataaagaga
     7201 gaatttttta aacatagaaa gtcctataaa aaaccgcttg aatacaagcg gttgaattta
     7261 aacatatttt tgcaattaga tcgctttagt tgcttttggc aagctgattt ttttctcttc
     7321 gctttggcgg taaagcacta aaatgtgccc aatggtttgt acttcagccg ctttagtttc
     7381 acgcagaatg gcatcaataa ttaattgttt ggtttcacga tcagccccgg caactttaac
     7441 tttaatcagt tcgtggtggt ctaaggcgtt atcgatttca gctaacacgc cttctgttaa
     7501 gccgttaccc cccagcatca ccactgggct taaatgatga gccaagcctt ttaggaattg
     7561 tttttgtttg gttgttaatg taattgacat tctttttccg ttaatttgtt ttgttgataa
     7621 aatttggcgt agaaaatagc acaaaaacca ctatttttaa agtaaaatgc aacgaatagc
     7681 agaaaaacac acgacaaaaa gagagaagga taaactatgc cgacattaag tgttgcgatg
     7741 attgtcaaaa atgaagctca agatttacgg acttgccttg acacagtaaa agattgggta
     7801 gatgaaattg tgattttaga tagtggcagt actgatgaaa caccacaaat tgctgctgaa
     7861 tacaacgcaa ggttctacac taacaccgat tggcaaggct ttggtaaaca gcgtcagtta
     7921 gctcaacaat atgtgaccag tgattatgta ctttggcttg atgcagatga gcgagtgaca
     7981 gccgaactga agcaatctat tttacaagcg gtcaaaaatg atgaaaaaaa taccgtttac
     8041 aaaatcagcc gcttaagtga agtatttggc aaacaaattc gccactcggg ttggtatccg
     8101 gattgtgtgg tgagattgta tcgcacggaa tttgccaaat atggtgatga attggttcac
     8161 gaaaaagtac attacccaaa agaggcaaaa atccagaaac tacaaggcga tttgctgcat
     8221 ttcacctata aagatattca ccactatttg gtaaaatcgg caagttacgc taaagcgtgg
     8281 gcaatccaac gggcaaatgc aggcaaaaca gccagcttat ttgatggcgt gactcacgcc
     8341 atcggctgtt ttgtgaaaat gtatctatta aaagccggtt ttttagatgg caagcaagga
     8401 tttttattgg cagttctttc ggcacattct actttcgtaa aatatgcgga tttgtggaat
     8461 cgtactcgga aatgatgctt ttcagtactc tctccctcta gtgggagaga gatagataaa
     8521 attgcgttta gcaattttat cagagagagg gagtattcca cattctccga ataaacggta
     8581 ctatcgtttt taatgcctca gaaatagaaa ttaaagttaa attcatttgt gaggctttat
     8641 cagacggtgg gcaaaatgca atgtgtttat tcgcatcatt aatcggtttc caacggcgtg
     8701 gaatggcaga aagtcggctc gggtagaagc caatagttgg aatatttaac gccccactaa
     8761 tatgtaacgg accggttgag ccggcaataa acaaatctgc acacgcaagc gaatgtgtaa
     8821 aatcttgtaa cccctcattt ttatcataaa ccaccacgtt gttgtgattg atttgttgag
     8881 ctaactgctg agctttttcg ctttcgcctt tccctgcggt gagtaccaca tagcaatcaa
     8941 attctgccaa aataccttga attaactctg catactgttc tatagataaa ttatttgccg
     9001 aaccaccggt tgaactatgc acaaataccc actttttaac ggtggtaata ttgagttgtt
     9061 cgcataattt tgctttttgt gcctggacgg tttgctgttc aacacttaag tatggcgtgt
     9121 gaggttcaac aatcggtata ttgtgtttgc ttaaaaaagc acgggctaaa tccaaattat
     9181 attcggattc cgcttttgct gactgagaac ggcgttgggt taaacggtgg ttatataaaa
     9241 attggaataa tttagttgct ggagcgaggc ggtatttaat cccactttgc caggtgagtt
     9301 tggcattatg ccagtctgaa acaaaacaaa taaccgcatc aaattgctca tttttaaccg
     9361 cttgtaaa
//
LOCUS       DANKBV010000086         9346 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.086, whole genome shotgun sequence.
ACCESSION   DANKBV010000086 DANKBV010000000
VERSION     DANKBV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.086"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(220..618)
                     /gene="mutT"
                     /locus_tag="PWY43_001691"
     CDS             complement(220..618)
                     /gene="mutT"
                     /locus_tag="PWY43_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDL2136556.1"
                     /translation="MSKPIIQVSAGIIRNEFGQIYLTQRLEGQDFAQSLEFPGGKVDP
                     NETPEEALKRELEEEIGIQVLSAFPYENFCFEYPTKIIEFFFYLVEEWVNEPYGREGQ
                     EGFWIAQSDLDEGAFPPANAQLIKRLKEHA"
     gene            complement(705..3431)
                     /gene="secA"
                     /locus_tag="PWY43_001692"
     CDS             complement(705..3431)
                     /gene="secA"
                     /locus_tag="PWY43_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL2136557.1"
                     /translation="MISKLLTSIFGSSNDRTLKRLRKRVAQINKLEPTFEQLSDEALK
                     DKTAEFKQRLADGASLDSLLPEAFATVREASKRVLGMRPFDVQLIGGMVLTGRNIAEM
                     RTGEGKTLTATLSCYLNALTGKGVHVVTVNDYLARRDAETNRPLFEFLGLTVGVNIPG
                     LPSDAKRAAYQADITYSTNSELGFDYLRDNLAHDKNERFQRELHYALVDEVDSILIDE
                     ARTPLIISGPAEDATQIYQAVDQIIPHLIAQDKEDSEEYTGEGDFTLDLKNKQAHLTE
                     RGMVKVEDFLTQMGLMEEGESLYHPARISLLHHVSAALRAHKLFERNVDYIVKDGEIV
                     IIDEHTGRTMAGRRWSDGLHQAIEAKERVAIQGENQTVASITYQNYFRLYEKLAGMTG
                     TADTEAFEFQQIYGLNTVVIPTNRPVIRDDRTDLMFKSEAEKFAAIITEIRDCIEREQ
                     PVLVGTASVEKSELLSAELTKAGIAHNVLNAKFHAQEAEIVAEAGAPGAVTIATNMAG
                     RGTDIVLGGNWKMEVAKLENPTQEQIDAIKAAWQERYDIVMQAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLDDALMRIYLNEGKLNMMRKAFTEEGEAMESKLLT
                     KVIASAQAKVEAHNFDGRKHLLQYDDVANEQRKVIYEQRNHLLETDNIASMIESIRQD
                     VFESTINQYIPPQSIEEMWNVPGLEERLRREFGLDLPISHWLETEKDLHEETLRERIL
                     NIAIAEYNAKEEIVGAEVMRNFEKGVMLQNLDELWKEHLASMDYLRKGIHLRGYAQKD
                     PKQEYKKESFAMFTNMLDTLKLNVISILSRIQVRSKEEVEEAQRQQAEMDAASTPVTQ
                     ATDSAAGSREENSAESLSEEELANLNIGRNDPCPCGSGKKYKHCHGNKARYS"
     gene            complement(3490..3810)
                     /locus_tag="PWY43_001693"
     CDS             complement(3490..3810)
                     /locus_tag="PWY43_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2547 family protein"
                     /protein_id="HDL2136558.1"
                     /translation="MSLFLKHFHRTPFWSQLFLGMVAILALPEIQVAYANESEQETVL
                     NQSFLQYAQSANDIEQQSLFIAELIHKIVEIKPQAVHFCEFFANSYRLDRQNAYPIRA
                     GPQT"
     gene            3931..4233
                     /locus_tag="PWY43_001694"
     CDS             3931..4233
                     /locus_tag="PWY43_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HDL2136559.1"
                     /translation="MKNSAPKKINDILQNSSLSRIVEKANFINDLNQKIQKRWPESYR
                     GLYRVVNLVDNQLIVSVKNATVRQGLILQQNMLLKQIQTDLPEITQLEFRVNPSVT"
     gene            4258..4632
                     /locus_tag="PWY43_001695"
     CDS             4258..4632
                     /locus_tag="PWY43_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HDL2136560.1"
                     /translation="MKKIALIGFTTAMLGACSLLPQKSVSGTYAGELPCADCEKIQAQ
                     LTLNADKTYQYDTVYFKDKKEYTYRDKGTYSWEPNKPNVIRLEKASGNLAFQVSEQHV
                     ELCDPNGNTVKNSNNYKLGKVR"
     gene            complement(4808..4897)
                     /locus_tag="PWY43_001696"
     tRNA            complement(4808..4897)
                     /locus_tag="PWY43_001696"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4861..4863),aa:Ser,seq:gga)
     gene            complement(4989..5300)
                     /locus_tag="PWY43_001697"
     CDS             complement(4989..5300)
                     /locus_tag="PWY43_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL2136561.1"
                     /translation="MSVEQTIIQKIKTEFQPIFFNVENESYMHSSGRGAESHFKLTLV
                     SDKFEGQRTVARHRAVYACLAEELANGVHALALHLYTQGEWEATGGLVPKSPNCLGVG
                     Q"
     gene            5392..5979
                     /locus_tag="PWY43_001698"
     CDS             5392..5979
                     /locus_tag="PWY43_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136562.1"
                     /translation="MKRSKKTLVLATTFVAALLAGCQSQSNTLTFSTPTPTAVFNTNN
                     QTAAVNVMTQDLRTSREIASYTKNGEVIRLTAVPEVAAMFQQAVQQDLNAKGFQIVQG
                     AANANVVVNIRKFFATVEQGNLRYKADAQVSVKVAVQGSKGNFSKNFNATRGYEGAFG
                     ASNDEIKKVLDQAYSDVVKAIYNDNEIANAIHQFK"
     gene            6151..6324
                     /locus_tag="PWY43_001699"
     CDS             6151..6324
                     /locus_tag="PWY43_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136563.1"
                     /translation="MNNPYHNVSQEYLDYLAKKVKQGKEDIKNGRIYSPEEVRKRVIA
                     AAFRGAENAKKVA"
     gene            6305..6595
                     /locus_tag="PWY43_001700"
     CDS             6305..6595
                     /locus_tag="PWY43_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2136564.1"
                     /translation="MPKKLHNIYYTEEALIDLENIAISVSEFTGYASSGIRILEELEN
                     SINNLAIFPTMGVKGAIINTRELYHNGYRIVCEVKENEISIITIVHCSRLYP"
     gene            6655..7386
                     /gene="rsmE"
                     /locus_tag="PWY43_001701"
     CDS             6655..7386
                     /gene="rsmE"
                     /locus_tag="PWY43_001701"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL2136565.1"
                     /translation="MRIPRIYHPQPLAGLQSCVLSEDAANHVGRVLRMKQGEQIILFD
                     GSNHVFHATLQAVEKKQIIAKIDSSELDDRESNLPIHLGQVISRGDRMEFTIQKSVEL
                     GVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
                     KEQDDMLKLNLHPRAKYTIRELPNVPKAGVRLLIGSEGGLSAEEIAMTESEGFTEVLL
                     GKRVLRTETASLAAITALQVCFGDI"
     gene            7386..7946
                     /locus_tag="PWY43_001702"
     CDS             7386..7946
                     /locus_tag="PWY43_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDL2136566.1"
                     /translation="MLENLQGKFLIATPEMDDDYFERTVVYICEHNPQGAMGVVINTP
                     TDLSVLELITRMDFQMANQRDYKKDQMVLSGGPVNQDRGFIIHTKTANDFIHTYPITD
                     KITLTTSGDVLDSFGTLASPEKFIVCLGCSTWRGSQLEQEIAQNYWIVSDANEKTLFE
                     TGYLERWAEAYEMMGLSSIPSRAGRA"
     gene            7946..8371
                     /gene="ruvX"
                     /locus_tag="PWY43_001703"
     CDS             7946..8371
                     /gene="ruvX"
                     /locus_tag="PWY43_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL2136567.1"
                     /translation="MTETTTKTVIAFDFGTNSIGCAVGQSITGTAQGLTTFKAQDGIP
                     NWEEIGKLLQDWKPDMLVVGLPLNMDGTEQPLTQRARKFANRLNGRFNLPVELQDERL
                     TTVEAKAEIFSRGGYKALKKDKVDSISACLILESWFENH"
     gene            8418..>9346
                     /locus_tag="PWY43_001704"
     CDS             8418..>9346
                     /locus_tag="PWY43_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2136568.1"
                     /translation="MTHSTKTLVAIASIIIILAGIKLSNEIVVPFLLSLFIAIICSPM
                     IKFMTKRKVPLWLAITILLIVFILGFSFLAGIINNTLQEFTNAIPQYKIQLSSRIQVI
                     LELAHRWHIPLTLSQEEIMSKFDPSSIMNLVSKLLMGLSGALSNIFVIFLVVIFMLLE
                     MPVAKYKLALALSPDDDLSNEKEYINQVLEGVIGYLGVKTFVSLLTGGLVWILLEILG
                     VEYAILWAILTFLLNYVPNIGSILAGIPIVIQALVLNGFSIGLGTLIGIVVINMVVGN
                     ALEPKLMGKKLGLSTLVVFLSLLFWGWLLGTVG"
BASE COUNT         2873 a         1916 c         1792 g         2765 t
ORIGIN      
        1 attaccattt tttaatgacg ctcctcaata atataagttg tgctaaatcc gatacaatca
       61 ttatttatta ggtataccaa gcggtcattt ttactaaaac tctgcaaaaa agaaagggcg
      121 aatagtaatt cgcccctaca tcagctttat aaatgtctca atagcgtatc caataaaaaa
      181 ccgcagctaa attactttag atacagttct caatacaaat tatgcgtgtt cttttaagcg
      241 tttaattaat tgggcatttg ccggtgggaa agctccctca tctaaatccg attgtgcgat
      301 ccaaaaacct tcttgccctt ctcttccgta aggttcattt acccactctt ccaccaaata
      361 aaagaaaaat tcaatgattt ttgttggata ttcaaagcaa aaattttcat acggaaaagc
      421 gcttaacact tgaataccaa tttcttcttc taattctcgt tttagtgctt cttcaggggt
      481 ttcattggga tcgactttcc ccccgggaaa ttcaagagac tgagcaaaat cttgcccttc
      541 taaacgttgg gttaagtaaa tttgcccaaa ttcgttacga ataatccctg ctgagacttg
      601 aataatcggt tttgacatag tttccttcac tctttctata aattcaagcg gtcatattaa
      661 gcagaaaatt tgcaaattcc tgactgaata tgaccgcttg ttaattacga atatctcgct
      721 ttgttaccat ggcaatgctt atattttttg cccgaaccac aagggcaagg gtcatttcga
      781 ccaatattta agtttgccaa ctcttcttca cttaagctct ctgcgctatt ttcttctcta
      841 ctaccagcag cagaatcggt agcttgtgta actggcgtac ttgcagcgtc catttcagct
      901 tgctggcgtt gcgcttcttc cacctcttct ttgctacgca cttgaatacg gcttaaaatg
      961 ctaattacat tcaattttaa ggtatcaagc atattggtaa acatcgcaaa agattccttt
     1021 ttatactctt gcttcggatc tttttgtgca taaccacgca agtgaatacc tttgcgtaaa
     1081 taatccattg aggctagatg ctctttccaa agctcatcta agttttgtag cattacacct
     1141 ttttcaaaat tacgcattac ctctgcgcct actatttctt ccttcgcatt gtactctgca
     1201 atcgcaatat ttaagatacg ttcacgcaaa gtctcttcgt gcaagtcttt ttcagtttca
     1261 agccagtgag aaatcggtaa atccaaccca aattcacggc gtaaacgctc ttctaagccc
     1321 ggcacattcc acatttcctc gatagattga ggcggaatat attggttaat cgtgctttca
     1381 aatacatctt ggcgaatgct ttcgatcatt gaggcaatat tatctgtttc taatagatga
     1441 ttgcgttgtt cgtaaattac tttacgctgt tcattggcaa catcatcata ttgtaataaa
     1501 tgcttacgcc catcaaagtt atgcgcttct actttcgcct gtgcagaggc aatcactttg
     1561 gttaacagtt tagactccat cgcttcacct tcttcggtaa aggctttacg catcatattt
     1621 aatttacctt cattgaggta aattctcatt agggcatcat ctaaagagag atagaaacgt
     1681 gatgaccccg ggtcaccttg acgaccagaa cgacctcgca actggttgtc gatacggcgg
     1741 gattcgtggc gttctgtacc aataatatgt aaaccaccgg cttgcattac gatgtcataa
     1801 cgctcttgcc acgcagcttt aatcgcatca atttgttctt gtgtcgggtt ctcaagcttg
     1861 gctacttcca ttttccagtt accgccaagc acaatatccg taccacgacc agccatattg
     1921 gttgcgatag taaccgcccc cggagctccc gcttccgcta caatttcagc ctcttgagcg
     1981 tggaatttcg catttaatac gttatgagca ataccggctt tggttaattc tgccgataat
     2041 aactctgatt tttccaccga tgctgtgcca accaaaacag gctgttcacg ctcgatacag
     2101 tctctaattt cagtaataat cgccgcaaat ttctccgctt cgcttttaaa cattaagtct
     2161 gtacggtcat cacggatcac cggtctgttg gttggaatca ccaccgtatt taagccataa
     2221 atttgctgga attcaaaggc ttcggtgtct gccgtaccag tcataccggc taatttttcg
     2281 tacaaacgga aatagttttg gtaagtaatt gaggcaacag tttggttttc gccctgaata
     2341 gccacacgct ctttagcttc aatcgcttgg tgtaagccgt cagaccaacg acgtccagcc
     2401 attgtacgac cggtgtgctc atcaataatc acaatttcac cgtcttttac aatgtaatct
     2461 acgttgcgtt caaataattt atgggcacgc aatgctgcac tcacatggtg taacagcgaa
     2521 atacgagcag ggtgatataa actttcgccc tcttccatta accccatttg agttaagaaa
     2581 tcctctactt tcaccatacc acgctcggtt aaatgagcct gtttgttttt cagatctaag
     2641 gtaaaatcgc cctcaccggt gtactcttca ctgtcttctt tatcttgagc aatcaaatgc
     2701 ggaatgattt gatctaccgc ttgataaatt tgggtcgcat cttccgcagg ccctgaaata
     2761 attaacggcg tacgagcttc atcaattaaa atagagtcta cttcatcaac taatgcgtag
     2821 tgtaactcac gttggaaacg ttcgttttta tcgtgagcta agttatcacg caagtaatca
     2881 aaacctaatt cgctgttagt cgaataggta atatctgctt ggtatgcagc acgttttgca
     2941 tcagaaggta accccggaat attcactcca acggttaagc ctaaaaactc aaataacgga
     3001 cggttggttt ccgcatcacg gcgggcgagg taatcattca ccgtaacaac atgaacacct
     3061 ttaccggtta aagcattcaa ataacaagat aaagttgcag ttagggtttt accttcccca
     3121 gtacgcattt ctgcaatatt acgccccgtt agcaccatac cgccgatcag ctgtacatca
     3181 aatggtcgca tacctaatac tcgtttgctt gcttctctca ctgtcgcaaa agcttcaggt
     3241 aataggctat ctagcgatgc accatctgca aggcgttgct taaactccgc tgttttatcc
     3301 tttaacgcct catcagataa ttgctcgaag gttggttcaa gcttattaat ttgtgccact
     3361 cgctttctta aacgttttaa agtgcgatcg ttgcttgagc caaaaataga agtcagtaat
     3421 tttgaaatca ttgtgttatt cctaaatata aaaatgggtt aatcaattaa tggtaaaatt
     3481 aaattatatt taggtttgtg gcccggctcg aatggggtaa gcattttgtc tatccaagcg
     3541 gtaggagttt gcaaaaaatt cgcaaaaatg caccgcttgt ggtttaattt ccacaatttt
     3601 atggattaac tctgcaataa aaagcgattg ttgctcaata tcattagcgg attgagcata
     3661 ttgaagaaaa gattgattaa gaacggtttc ctgttcactt tcattggcgt acgcaacctg
     3721 aatttcaggt aaagcaagta tcgcaaccat tcccaaaaag agttgcgacc aaaaaggcgt
     3781 tcgatgaaag tgttttaaaa ataagctcat taaattaaga taaacgaaaa aattgggcta
     3841 attatacgct aaagtaaagc cgaatgagta aattattggg tcaagtatcg taaaatcaag
     3901 cctaaaataa gacgaaatgt aaagaaacag atgaaaaatt cagcacctaa aaagattaat
     3961 gacattttac agaactcaag cttaagccgt attgtcgaaa aagcaaactt cattaacgat
     4021 ttaaaccaaa aaattcagaa aagatggcca gaatcttatc gtgggcttta ccgagtagtg
     4081 aatttagttg ataatcagct tattgtgagc gttaagaatg caaccgtacg gcaaggttta
     4141 atcttgcaac aaaatatgtt actaaaacag atacaaaccg atttacctga aataacccaa
     4201 cttgaattta gagtaaatcc gagtgttaca taatttaatt ttgaagagga aaatagtatg
     4261 aaaaaaatcg cactcattgg ttttaccacc gcaatgctag gggcttgctc attgctacca
     4321 caaaaaagcg ttagcggtac ttatgcaggt gaattaccgt gtgctgactg tgagaaaatc
     4381 caagcccagc taaccttaaa tgcagataaa acctaccaat atgatacggt atattttaaa
     4441 gataaaaaag aatacactta ccgagacaaa ggcacataca gttgggaacc caataagcca
     4501 aatgtgattc gtctggaaaa agcatcaggc aatcttgcct tccaagtgtc tgaacaacac
     4561 gttgaacttt gcgacccaaa tggcaacacg gtaaaaaaca gcaataacta taaactaggt
     4621 aaagtacgtt aaaagcagat aaaaaggatt cttaggaatc ctttttcttt gtaatctatc
     4681 gccgatcgca taaaaaaatc cccaagcaat gtttgctcgg ggataaccta aaaatgaaaa
     4741 agtattatct actttaactt aaccaaggaa atatctatga aaacagtaag gaatctttca
     4801 gtatgaatgg cggtgagagg gggattcgaa cccccgatac agtttcccat atacacgctt
     4861 tccaggcgtg ctccttcaac cactcggaca tctcaccgaa tcaacaaaaa gatggcgtaa
     4921 atatagcaat tttacacggt tctttcaagt attttcttca caaaataaaa taaatcattt
     4981 ttatccactt attgcccaac acctaagcaa tttggcgatt taggtacaag tccgccagtc
     5041 gcttcccatt caccttgggt atataggtgc aaagcaagtg cgtgaacgcc attagctaat
     5101 tcctcagcta agcaagcata gacagcacga tgacgtgcaa ctgttctctg cccttcaaat
     5161 ttatctgaca ccaaagttaa tttaaaatgc gattctgctc ctctacctga gctgtgcata
     5221 tagctttcgt tttcgacatt aaagaaaatc ggctgaaatt ctgtcttaat tttttgaata
     5281 attgtctgtt ctaccgacat tttttgctct cgttcattga aaatcaaaac gaaataatac
     5341 tatactaagc agaaattttc tcgaaattta ttcaatatag aaggatttac tatgaaacgc
     5401 tctaaaaaaa cgttagtgtt agccactact tttgttgccg cattactagc cggctgccaa
     5461 agccaatcca acacattaac attctctacc ccaacgccaa ctgctgtatt taacacaaac
     5521 aatcaaacgg ctgcggtgaa tgtaatgaca caagatttac gcacctcgcg tgaaatcgca
     5581 agctacacta aaaacggaga ggtcattcgt ttaaccgcag tgcctgaagt tgctgcaatg
     5641 ttccaacaag ccgtccaaca agacctgaat gcaaaaggtt tccaaattgt acaaggtgca
     5701 gcgaatgcca atgtggtagt gaatattcgt aaattctttg ccacagtaga acaaggaaac
     5761 ttacgctaca aagccgatgc acaagtgagt gtaaaagtgg cagtgcaagg ttctaaagga
     5821 aactttagca aaaacttcaa cgctacccgt ggttacgaag gtgcatttgg tgcaagcaat
     5881 gatgaaatca agaaagtgtt agaccaagcc tattctgatg tggtgaaagc tatttacaac
     5941 gataatgaaa tcgccaatgc aattcaccag ttcaaataat taattttttc tggcaagcgg
     6001 tcatctttct tcgatttttt gcaaaaaacg tggaaaaatg accgcttgtt gttttctcgt
     6061 tttgtgatgt ggctcgaaaa ataagataaa ttaacttgat taaacgatca gtagccccca
     6121 caatagagac tcatcgcagg gaggaaaaat atgaataatc cttatcataa tgttagccaa
     6181 gaatatcttg attatcttgc taaaaaagta aagcaaggaa aggaagatat taaaaacggt
     6241 cggatttatt cacccgaaga ggtcagaaaa agagttattg ctgccgcttt tagaggtgct
     6301 gaaaatgcca aaaaagttgc ataatattta ttatactgaa gaggccttaa tagatttaga
     6361 gaatattgcc atctcagtta gtgaatttac cggttatgca agtagtggaa ttaggatact
     6421 agaagaattg gaaaatagta ttaataattt agccattttt cctacaatgg gtgtaaaagg
     6481 tgcaattatt aatacgagag aactatatca taacggctat agaatagtgt gtgaagttaa
     6541 agagaatgaa atttcgatta ttactattgt gcattgttca agactttatc cttaacccgt
     6601 tttaacttac ctatggcaga caatcgtctg cctttaattt ataactagat aattatgaga
     6661 atcccaagaa tttaccaccc acagccgtta gctggtcttc aaagttgcgt gttaagtgaa
     6721 gatgccgcta accacgttgg gcgagtgcta cgaatgaaac aaggagagca aatcatttta
     6781 tttgatggtt caaaccacgt tttccacgct acgttacaag cggtcgaaaa aaagcaaatt
     6841 attgcaaaaa ttgattcaag tgaattagat gaccgagaat ccaacctgcc gatccattta
     6901 gggcaagtga tttcccgagg cgatagaatg gaatttacca ttcaaaaatc ggtggagctg
     6961 ggggtaaaaa ctattacccc actttggtcg gaacgttgtg gcgtgaaact caatgatgaa
     7021 cggcaagata aaaaattaca gcaatggcag aaaattgcga ttgccgcctg tgaacaatgc
     7081 ggacgaaatg aaatccctga aattcgcccg attatgaaac tcaccgactg gtgcaaagaa
     7141 caagacgata tgttgaaact gaatttgcac ccaagagcca aatataccat tcgtgaactg
     7201 ccgaatgtgc cgaaagctgg tgttcgttta ctgattggct ctgagggcgg attatctgcc
     7261 gaagaaatcg ccatgaccga gagcgaaggt tttactgaag tattactggg taaacgagtg
     7321 ttacgcacag aaaccgcctc acttgcggca attactgcgt tacaagtttg ttttggagat
     7381 atttaatgtt agagaattta caaggtaaat ttttaattgc cacgccggaa atggacgatg
     7441 actatttcga gcgtacggtg gtctatattt gcgaacataa tccgcaaggt gcaatgggag
     7501 tagtaattaa cactcctacc gatctgtcgg ttctagagct gattacccga atggatttcc
     7561 aaatggcgaa ccagcgtgat tacaaaaaag accaaatggt gttaagcggt gggccggtaa
     7621 atcaagatcg tggctttatt attcacacca aaaccgccaa tgattttatt cacacttatc
     7681 cgatcaccga taaaattacg ctcactacct ccggtgatgt gttagactcc tttggcacac
     7741 tggcttcacc tgaaaaattt attgtgtgtt taggttgttc cacttggcgt ggcagccagc
     7801 ttgagcaaga aattgctcaa aattattgga ttgtgtctga tgcgaatgaa aaaacgctgt
     7861 ttgaaaccgg ttatttagag cgttgggcag aagcctatga aatgatgggg ctttcaagca
     7921 taccgtcaag agctgggaga gcttaatgac agaaaccaca acaaaaaccg tgattgcctt
     7981 tgattttggc acaaatagca tcggctgtgc agttggacaa agtattaccg gcacagctca
     8041 aggtttaact acatttaaag cccaagacgg tattccaaac tgggaagaga tcggaaaact
     8101 cttgcaagat tggaaaccag atatgctggt tgttgggcta cccttgaata tggacggcac
     8161 agagcagcct ttaactcagc gtgccagaaa atttgccaat cgcttaaacg gacgctttaa
     8221 tctgccggtt gagctacaag atgaacgcct cactactgtt gaggcaaaag cggaaatttt
     8281 ttctcgtggt ggttacaaag cattaaaaaa ggataaagtg gattccattt ccgcttgttt
     8341 aattttagaa agttggtttg agaatcacta atttgtaaaa aattgctgat ttttaaccgc
     8401 ttgtaaaagg aaaaattatg acacattcca ccaaaactct cgtggcgata gcctccatta
     8461 tcattattct ggcagggatt aaattatcca atgaaattgt tgtgccattt ttactttcgc
     8521 tatttattgc cattatttgt tcaccaatga tcaaatttat gactaagcgt aaagtgccat
     8581 tatggttagc cattaccatt ttacttattg tttttatctt aggattcagt ttcttagcag
     8641 gcattattaa taacaccttg caagaattta ctaatgctat tccgcaatac aaaattcagc
     8701 tttcatctag aatccaggtg attctagaac tcgcccatag atggcatatt ccacttaccc
     8761 tctcacaaga agagattatg agcaaatttg acccaagctc aataatgaat ttggtcagca
     8821 aactgctaat ggggctatcc ggtgcattat cgaatatttt tgtgattttc ttagtcgtca
     8881 tttttatgct attggaaatg ccggttgcca aatataaatt agccctcgca cttagcccag
     8941 atgatgattt aagcaacgaa aaagagtata tcaaccaagt acttgaaggg gtgattggtt
     9001 accttggagt taaaaccttt gtgagcctac tcaccggagg gttagtttgg atcttactag
     9061 aaattctagg cgtagaatat gcgattttat gggcaattct aaccttcctg cttaactacg
     9121 tcccgaatat cggctctatt ttggcgggaa ttccaattgt gattcaagcc ttggtactca
     9181 atggcttttc tataggatta ggcactttaa tcggcattgt cgtgattaat atggttgtgg
     9241 gtaatgcgct tgaacctaaa ttaatgggca agaaactcgg gctttctacc cttgtagtgt
     9301 tcctctcact tctattttgg gggtggttac ttggcaccgt aggaat
//
LOCUS       DANKBV010000087         9223 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.087, whole genome shotgun sequence.
ACCESSION   DANKBV010000087 DANKBV010000000
VERSION     DANKBV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.087"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(18..758)
                     /gene="pflA"
                     /locus_tag="PWY43_001705"
     CDS             complement(18..758)
                     /gene="pflA"
                     /locus_tag="PWY43_001705"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDL2136569.1"
                     /translation="MSVVGRIHSFESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWD
                     LDGGKEITVEELMKEVTTYKHFMKATGGGVTASGGEAVLQMEFVRDWFRACKAEGINT
                     CLDTNGFVRHYSPVVDEMLEVTDLVLLDLKQLNDEVHQDLVGVSNKRTLDFARYLHKL
                     NKPTWVRYVVVPGYTDDDDSAHRLGQFIQGMDNIEKVELLPYHRLGAHKWETLGYKYE
                     LEGVMPPSKEDLERIQKIIESYGHTVKF"
     gene            complement(1007..2032)
                     /locus_tag="PWY43_001706"
     CDS             complement(1007..2032)
                     /locus_tag="PWY43_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL2136570.1"
                     /translation="MSKQDLIRSSVAEYLTFITATGESQVDAVYADENVWLSQKMMAV
                     LYDVDVRTISYHLKKIFSDNELAENSVIQFFWITAQDGKNYHTKHYNLSAIIAVGYKV
                     NSERAVQFRKWATEIVQSFAIKGFAMDDERLKNDGTILGKKYFEEQLARIREIRLSER
                     KFYQKITDIYATAVDYDRTATATKRFFATVQNKLHWAIHGHTAAELIMARANAEKPNM
                     GLTNWKDAPQGKIYPFDVVVAKNYLSDNELAQLQRLVSAYLDMAEDMAERQIPMTMAD
                     WETRLNRFLAATDREILQDAGKVTAEIAKSFALSEFEKYRVKQDLTYESDFDLLVKEV
                     AAKYHAG"
     gene            complement(2267..4591)
                     /gene="pflB"
                     /locus_tag="PWY43_001707"
     CDS             complement(2267..4591)
                     /gene="pflB"
                     /locus_tag="PWY43_001707"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL2136571.1"
                     /translation="MTQLTDAQQKAWEGFAAGEWQTEVNVRDFIQKNYTPYEGDESFL
                     AEVTPATSKLWADVMEMIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQT
                     DAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKQRQFASLQARLESGEDIQATI
                     QLREEIAEQHRALGKMKEMAASYGFDISGPATNAQEAVQWTYFAYLAAVKSQNGAAMS
                     FGRVSSFLDIYIERDLRAGKITEQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMW
                     ATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEQLPEAFKRYCAKVS
                     IDTSSVQYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGG
                     VDEKSGDQVGPKTEAITSEYLDYDDVMTRLDSFMDWLATQYVTALNIIHFMHDKYAYE
                     AALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPIRTDIEIKNKAGEVVGVAK
                     NVATDFEIEGEYPQFGNNDNRVDDIAVDLVERFMKKIQKLKTYRNATPTQSVLTITSN
                     VVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTF
                     SIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNVLNRDTLLDAVEHPEKY
                     PQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM"
     gene            complement(4681..5505)
                     /gene="focA"
                     /locus_tag="PWY43_001708"
     CDS             complement(4681..5505)
                     /gene="focA"
                     /locus_tag="PWY43_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDL2136572.1"
                     /translation="MFSPAEMAKIAEDGAVYKATKNQLYSFLSAITAGGFIAIAFVFY
                     TTTQVGASQMPWGMAKLIGGLVFSLGVIMCVVFGAELFTSSTLTIVARASQRINWLQM
                     FKNWVVVYFGNFVGAISIVLLIWFSGQIMAAKGQWGLVILNTAQHKIHHQWFEAFCLG
                     IFCNIMVCIAVWMSYAGKSLTDKAFIMMLPIAMFVASGFEHCVANMFMIPMGIAISHF
                     AGPEFWTAINVDQTQFADLDLYHFVFKNLIPATLGNIVGGVFFIGLMQWFLYIRKH"
     gene            5706..6200
                     /locus_tag="PWY43_001709"
     CDS             5706..6200
                     /locus_tag="PWY43_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL2136573.1"
                     /translation="MTEYLTSFFSLFFSEQNQLFTMFLSGFLSATLLPGNSEIIFTTL
                     ASQHLITYGSLSSFSLFNLLLVATLGNSLGSITTYAIGLLLPRPQNTTNRYSQWALAK
                     SEKYGVLVLLFSWSPIVGDIFCGIAGWLRFSLWQSILFIVIGKLLRYLFLLATLYSVL
                     PHIL"
     gene            complement(6254..7123)
                     /gene="htpX"
                     /locus_tag="PWY43_001710"
     CDS             complement(6254..7123)
                     /gene="htpX"
                     /locus_tag="PWY43_001710"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDL2136574.1"
                     /translation="MAKRVILFLLTNLAITFVLGIVLNLIFKVTGIQGQSTAGILVLS
                     LVFGFSGSLISLFMSKSMALRSVGAEVITQPRNEAEQWLFNTVQRQAQQANIPMPDIA
                     IYHSADVNAFATGATKSNSLVAVSTGLLNSMTKDEAEAVVAHEVAHIANGDMVTMTLL
                     QGVLNTFVIFLARIISTMAASSRDENGNSSTNTLVFWVVDIVLQMVFGVLATMIAMWF
                     SRQREFRADAGSAQLVGKEKMIAALQRLQRVHEPQEMEGSLAAFMINGSRGKELFMSH
                     PPLEKRIEALRNL"
     gene            complement(7211..8293)
                     /gene="dinB"
                     /locus_tag="PWY43_001711"
     CDS             complement(7211..8293)
                     /gene="dinB"
                     /locus_tag="PWY43_001711"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL2136575.1"
                     /translation="MSIQAKHKRKIIHIDMDCFYAAVEMRENPALVGKPVAVGGNANQ
                     RGVLTTCNYEARKFGLHSAMPTATALKKCPNLILLPVNMALYKLVSAQIHEIFHRYTD
                     VIEALSLDEAYLDVTECERYFGSATWIAQAIRNDIFNELHLTASAGVAPLKFLAKIAS
                     DQNKPNGQCVITPDDVQNFVKNLPLAKIPGVGKVTNEKLKQLGFETCGDIQQAEKAVI
                     YRAFGKFGQRLWDFSHGIDERAVESYRERKSLAVENTLLHDISTLEQAEKIVADLFDK
                     LVFRLQRNWGDTPLTEFKKLGIKLKFDDFSQTTLERTTDGFAYERFGELLQQIFTRRK
                     ERKVRLIGLSVHFPEKKVNKQLNLWE"
     gene            8416..>9223
                     /locus_tag="PWY43_001712"
     CDS             8416..>9223
                     /locus_tag="PWY43_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HDL2136576.1"
                     /translation="MLYGLAIVLIPLFLGYLITLKNHRYMAIVNRVVSLCLYLILLVM
                     GISLGQIDNILTELPQIGGTAFSLVVILLLSNIIGLVIYDKLQPMKRDKVNQDQLPSR
                     LHLLIDSFKLIGITLLGGIIGYFTKGVVDFPLHASTYVLEVMIFGVGIQLRNSGIPLR
                     EVFFNKRGIYTSIVMVASSLIGGVISAFTLDLSLAKGLTFASAFGWYSLSSVLVNDAW
                     GPIYGSIAFFNDISREILCLFLVPLFMRQFPSTAVGIGGATSLDCMLPIIQ"
BASE COUNT         2605 a         2081 c         1764 g         2773 t
ORIGIN      
        1 ttcaggattt tacaaaatta aaatttcacg gtatgcccgt aactttcaat gattttttgg
       61 atacgctcta aatcctcttt agacggtggc attacccctt ccagctcata tttatagcct
      121 aaagtttccc atttatgagc tcctaaacga tgataaggca gaagttccac tttctcaatg
      181 ttatccattc cttgaataaa ctgccctaaa cgatgggcag aatcatcgtc atcggtataa
      241 cccggcacga ccacatagcg aacccaagtc ggtttattta atttgtgtag ataacgggcg
      301 aaatcaaggg tacgcttatt cgacaccccg actaaatctt ggtgaacttc atcattgagc
      361 tgttttaaat ccagcaagac caaatcggtc acttcaagca tttcatccac caccgggcta
      421 taatggcgaa caaaaccatt ggtatctaag caggtattaa tgccttcagc cttacaggca
      481 cgaaaccaat cccgcacaaa ctccatttgc aacaccgctt ccccacccga agctgttaca
      541 ccaccgccgg ttgctttcat aaaatgctta taagtcgtga cttctttcat cagctcttca
      601 accgtaattt ccttaccacc atcaagatcc caagtatcac gattatggca atatttacac
      661 cgcatcaaac agccttgcaa gaacagaata aaacgaatcc ccggtccatc taccgtaccg
      721 caggattcaa aagaatggat acgtccaaca acagacattt gtcacctcaa ccctcaaaaa
      781 attttgagaa attgtagcaa atttttatgt tgttcgcaga aataaatcac accggtatat
      841 tttaagaaag gagaaagtca tactattttt tgcctatttc aggaataggc ttttattaat
      901 ctattgactc catcaacata tcaaccaaat atgtttaaat ttttgcattt tttaaagcta
      961 ttttgaccgc ttacaagcag tcggtttttc tgaaaacttt gcaaacctag ccggcgtggt
     1021 atttggcggc gacctctttt accagtaagt cgaaatcgct ttcgtaggtg agatcctgct
     1081 tgactcggta tttttcaaat tcgctgaggg cgaaagattt agcaatttca gcggtaactt
     1141 tgccggcatc ttgtaagatt tctcggtcgg tggcggcaag aaagcggtta aggcgggttt
     1201 cccagtctgc cattgtcatc ggaatttggc gttctgccat atcttccgcc atatccaaat
     1261 aggcagagac cagccgttgt agttgtgcta attcattatc cgataaataa tttttggcaa
     1321 ccaccacatc aaaggggtaa attttgcctt gcggagcgtc tttccaattc gttagcccca
     1381 tattcggctt ttcagcgttg gctcgtgcca taatcagctc ggcggcggta tgcccgtgaa
     1441 ttgcccaatg gagcttgttc tgcacggtgg caaaaaagcg tttggtggcg gttgcggtgc
     1501 ggtcgtagtc cacggcggta gcgtaaatgt cggtgatttt ttggtagaac ttgcgttcgg
     1561 ataaccgaat ttcccgaata cgagcaagct gttcttcaaa gtattttttg cccaaaatcg
     1621 tgccgtcatt tttcagccgt tcatcgtcca tcgcaaagcc tttaatcgca aagctctgca
     1681 cgatttcggt tgcccatttg cgaaactgca ccgctcgctc ggaattgact ttataaccca
     1741 ccgcaataat tgccgacaga ttataatgct tggtgtggta gtttttgccg tcttgggcag
     1801 ttatccaaaa aaattggata actgaatttt cagctaactc attgtcacta aagatttttt
     1861 tgagatgata gctaatggtt cgtacatcaa catcatacaa caccgccatc attttttggc
     1921 taagccatac attttcatcg gcgtaaacgg catcaacttg gctttcgccg gtagctgtaa
     1981 taaaagtgag gtattccgcc acagaggaac gaattaaatc ttgcttagac atctctttct
     2041 cccatataga acactgtaat tataaacaat attacgaaag ctgtgtaaat gaatagccaa
     2101 tttttcgagc gagatcacaa aaaaaccgct tgcggcataa atgctacaag cggttgatta
     2161 ttactcacaa aattgtttcc aattttgttc gctcctacgg agccgtcggc aaagccaacg
     2221 ttcaaaacgc aatgcgtttt gtggctaaaa gttttgcaaa ttgtaattac atactttcag
     2281 tgaaggtgcg ggtaattacg tccatttgtt gctctttggt taaagagttg aaacgtaccg
     2341 catagcctga aacacgaatc gttaattgcg gatatttttc agggtgttct accgcatcta
     2401 ataatgtatc acggtttaac acgttcacat ttaagtgctg accgccttct acagttgctt
     2461 cgtggtggaa gtagccgtcc attaagcctg caaggttacg tttttgggct tcgtaatctt
     2521 tacctaacgc atttggtacg atagagaaag tataagaaat accatctttc gcataggcaa
     2581 acggtaattt cgccacagaa gttaatgaag ctaccgcacc tttctggtca cgaccgtgca
     2641 ttgggttcgc acccggtccg aatggagcac ctgagcgacg accgtccggt gtattaccgg
     2701 ttttcttacc atataccacg ttagaagtga tagtaagcac agactgagtc ggcgttgcat
     2761 tgcggtatgt tttgagtttt tgaattttct tcataaaacg ttcaactaaa tcaactgcaa
     2821 tgtcgtccac acggttatcg ttgttaccga attgcggata ttcaccctca atttcaaagt
     2881 cggttgctac gtttttcgct acaccaacca cttcgccagc tttgtttttg atttcaatat
     2941 cggtacggat tggttttact ttcgcatatt taatggctga aagtgagtct gccgctactg
     3001 aaagccctgc gataccacac gccattgtgc ggaacacatc acggtcgtga agagccatta
     3061 atgccgcttc gtatgcatat ttatcgtgca taaagtggat aatgttcagt gctgtcacat
     3121 attgggttgc aagccaatcc ataaagctgt ctaagcgggt cattacatca tcgtaatcta
     3181 agtattcact tgtaattgct tcagttttag gccctacttg gtcgcctgat ttctcatcaa
     3241 caccgccatt gattgcgtat aacaaggttt tcgctaagtt tgcacgagcg ccgaagaact
     3301 gcatcatttt acccacgatc attggcgata cgcagcacgc aatcgcatag tcatcgtttt
     3361 ggaaatccgg acgcattaag tcatcgtttt catattgaac tgatgaagta tcaatagaca
     3421 ctttcgcaca gtagcgttta aatgcttccg gtaattgttc agaccaaaga atagttaagt
     3481 ttggctccgg tgacggtccc attgtgtaaa gggtatgtaa aatacggaag ctgtttttgg
     3541 ttactaatgt gcgaccgtct aaacccatac cggctaaggt ttcggttgcc cacattggat
     3601 cgcctgagaa taattgatcg tattctggag tacgcaagaa acgcaccata cgcaatttca
     3661 ttactaagtg gtcgattaat tcttgggctt cttgctcggt aattttaccg gctcttaagt
     3721 cacgttcgat ataaatatct aagaaagaag acacacgacc aaaagacatc gccgcaccgt
     3781 tttgcgattt taccgctgca aggtaagcga aataagtcca ttgcaccgct tcttgtgcat
     3841 tggttgccgg acctgaaata tcaaaaccgt agcttgctgc catctctttc attttgccta
     3901 aagcacgatg ttgctcggca atttcttcac gcagttgaat cgtggcttga atatcttcgc
     3961 cggattctaa acgagcttgt aatgaggcaa actggcgttg tttgtctttc attaagaaat
     4021 ccgcaccgta aagtgccata cggcggtagt caccgataat acgaccacga ccataagcat
     4081 ccggtaaacc ggtaattacg cccgatttac ggcaacgtaa aatatccgga gtgtaaacat
     4141 cgaatacccc ttggttatga gttttacgat attcagtaaa gatttgttct acttcaggtt
     4201 taagctcacg gcgataaact ttgcacgaac cttttaccat attgataccg ccaaacggca
     4261 taatggcacg ttttaatggg gcatctgttt gcagaccgac gattttttct aacgatttat
     4321 caatataacc cggtgcgtgt gaggtaatgg ttgatggcgt gtcgcagtca atatcatacg
     4381 gctcgtgtgt tttgttttct actttgatca tttccatcac atctgcccac aatttgctgg
     4441 ttgcaggtgt tacttcagct aggaaagact catcaccctc ataaggtgtg tagttttttt
     4501 ggataaaatc acgtacattt acttcagttt gccactcacc tgcggcaaaa ccttcccacg
     4561 ctttttgttg agcgtctgtt agttgagtca ttttaagtcc tctgtgttag taagttaaaa
     4621 cgggaaagcg gtaatttttt gcaaaaaatt tgcaaaattt gaccgcttga ttctgaaatc
     4681 ttagtgcttt ctaatgtata agaaccactg cattaagccg atgaaaaaca caccgccgac
     4741 aatattaccc agcgttgccg gaattaggtt cttaaacaca aaatgatata gatctaaatc
     4801 ggcaaattgc gtttggtcaa cattgattgc cgtccaaaat tcaggaccgg caaagtgaga
     4861 aatcgcaatt cccatcggaa tcataaacat atttgccaca cagtgttcaa agccggaagc
     4921 cacaaacatt gcaatcggta acatcataat aaaggcttta tcggttaggc ttttacctgc
     4981 ataagacatc cacaccgcaa tgcacaccat aatgttacag aaaatgccta aacagaaagc
     5041 ctcaaaccat tggtggtgaa ttttgtgctg tgcggtattt aaaataacca aaccccattg
     5101 ccctttggct gccataatct gaccactaaa ccaaatcaac aatacaatgg aaatcgcccc
     5161 aacaaagtta ccgaaataca ccactaccca gtttttaaac atctgtaacc agttaattct
     5221 ttggctggct ctggctacaa tcgtaagtgt tgatgaggta aaaagttctg ccccaaacac
     5281 cacacacata atcacgccga gtgaaaatac caaaccacca atcagtttag ccatacccca
     5341 aggcatttgg ctcgcaccca cttgagttgt ggtataaaat acaaatgcta ttgcaataaa
     5401 accgcccgca gtaatggcag agagaaagga atataattga ttttttgttg ctttataaac
     5461 cgcaccatct tctgcgattt ttgccatttc agcaggagaa aacatcagac aaccttaaca
     5521 atgagaattg aaaaaccgat atattatgta gaaattatag acctcttgag ataatcttca
     5581 aataaaaata ttacacaatc agaggtattt cgatgtcttt ttttgatata gaacaaaatc
     5641 cataaaaaac acaacattca agaaaattta taactacatt ttaataacaa accaactaca
     5701 attttatgac agaatatctc acttcctttt tcagtttatt cttttccgaa caaaatcaac
     5761 tctttactat gtttctgagt ggatttctaa gcgctaccct cttacccgga aattctgaaa
     5821 ttatcttcac taccttagca agccaacatc tcatcactta tggctcttta tcctcatttt
     5881 cactgttcaa tttactcttg gttgcaacct taggtaatag tttaggtagc ataaccactt
     5941 atgcgatagg gctattactc cctcgcccac agaatactac caatcgttat tcacaatggg
     6001 cattagccaa aagtgaaaaa tatggcgtgt tagtcttgct ttttagttgg tcaccgattg
     6061 taggcgatat tttctgtggc attgccggct ggctacggtt tagcctttgg caaagcattc
     6121 tctttattgt gattgggaaa ttgttacgtt atctgttttt gctggcaact ctctattcgg
     6181 tgttaccgca tattttatag aaaacaaaag cggctgtttt gcaaaaaatt ttacaaaaac
     6241 aaccgcttgt gctttataag tttcttaacg cttcaatacg tttttctaac ggagggtggc
     6301 tcataaacaa ctctttacca cggctaccgt taatcataaa ggcagcaaga gagccttcca
     6361 tttcttgcgg ttcgtgaaca cgttgcaagc gttgtaatgc cgcaatcatt ttctctttac
     6421 cgactaattg agcagagcct gcatctgcac gaaattcacg ttggcgagag aaccacattg
     6481 caatcattgt tgctaacaca ccaaacacca tctgtaacac aatatctact acccagaaca
     6541 ccagcgtatt ggtgcttgaa ttgccatttt cgtcacgtga acttgccgcc attgtcgaaa
     6601 taatacgagc taagaaaatg acgaaagtat ttaacacgcc ttgtaataaa gtcattgtaa
     6661 ccatatcgcc attcgcaatg tgcgccactt cgtgtgctac taccgcttca gcttcgtctt
     6721 tagtcattga attcagcaag ccggtgctga ctgcaactaa agaattactt ttcgttgctc
     6781 ctgtagcgaa tgcatttaca tcagccgaat ggtaaatcgc aatgtccggc attgggatat
     6841 tcgcttgctg agcctgacgt tgtacagtgt tgaataacca ttgctccgct tcgttacgag
     6901 gctgtgtaat cacctctgcc ccaaccgaac gcaatgccat tgatttcgac ataaatagcg
     6961 aaattaaaga cccactgaaa ccgaagacta acgaaagcac caaaataccg gcagtgcttt
     7021 gcccttgaat gccggtcact ttaaaaataa ggtttaatac gatccctaaa acaaaggtaa
     7081 tcgctaagtt agttaataaa aatagaatga cacgttttgc cataatgttt tcaatctcca
     7141 aaaatataga tgcaatgatt gcacgaataa gataatgact ttagccttaa tttcaagtca
     7201 ttatttacaa ttattcccac aaatttaact gtttattcac ttttttctcg ggaaagtgaa
     7261 cacttagacc gattaatcgc acctttcgtt cctttcttcg ggtaaaaatt tgctgtagca
     7321 actcgccaaa tcgctcatac gcaaagccat cggtggttcg ttcaagcgtg gtttggctaa
     7381 aatcatcaaa cttgagttta atgcctaact ttttaaactc agttaatggc gtatcgcccc
     7441 agtttcgttg caaccgaaaa accagtttgt cgaataaatc tgccacaatc ttttccgctt
     7501 gctcaagggt tgaaatatcg tgcaacagcg tattttccac cgccagcgat tttctttcac
     7561 gataagactc taccgctcgt tcatcaatac cgtggctaaa atcccaaagc cgttgcccga
     7621 atttgccaaa cgctcggtaa ataaccgctt tttctgcttg ctgaatatca ccacaggttt
     7681 caaagccaag ctgttttaat ttttcattgg ttactttccc cacacccgga atttttgcaa
     7741 gcggtagatt tttcacaaaa ttttgcacat catcaggcgt aatcacacat tgtccgttag
     7801 gtttgttttg atcggaggca atttttgcta aaaatttaag cggagcaact cctgccgagg
     7861 cagttaaatg caattcattg aaaatatcat tgcggattgc ttgagcaatc caagtggcgg
     7921 agccaaaata gcgttcgcat tcggttacat ctaaataggc ttcatcaagc gacaaggctt
     7981 caatcacatc cgtatagcga tgaaaaattt cgtgaatttg agcggaaacc aatttataca
     8041 acgccatatt caccggcaat aaaattaaat tcgggcattt cttcaacgca gttgccgttg
     8101 gcattgcact gtgtaagccg aattttcgag cctcgtaatt acaggttgtc agcacacctc
     8161 gttgattagc attgccgccc accgccaccg gcttaccgac caacgccggg ttttcccgca
     8221 tttcgaccgc tgcataaaaa caatccatat caatatggat aatttttcgc ttatgtttag
     8281 cttgaatcga catttgcaat attttcccca aaaacgaccg cttgcaacac aaataactgt
     8341 atataaaaac agttttattt tatgccctta ttgttagaat ttcaagttta aaaatagcga
     8401 taaaaaggat aaaaaatgct ctacggttta gccattgtgc tgattccact ttttctcggt
     8461 tacttaataa ccctcaaaaa ccaccgctat atggcaatag taaacagagt ggtcagcctc
     8521 tgcctttact tgattttatt agtgatgggg atttctctcg gacaaattga taacattctc
     8581 accgaactgc cacagattgg cggtacggcg tttagtttag tggtaatttt actcctttca
     8641 aacattatcg gcttggtgat ttacgacaaa ttacaaccga tgaaaagaga caaagtcaat
     8701 caagatcaac ttccctcccg cctgcatttg ttgattgatt cctttaaatt aatcggcatt
     8761 accttattag gcgggatcat cggctatttc accaaaggcg tggtggattt ccctctgcac
     8821 gccagcacct atgtgttaga agtgatgatt ttcggggtgg ggattcaact gcgaaacagc
     8881 ggtattcccc tgcgtgaagt gttctttaac aaacggggga tttatacctc gattgtaatg
     8941 gtggcaagtt ccctgattgg tggcgtgatt tcggcattca ccttagattt atcccttgct
     9001 aaaggcttaa cctttgcctc tgcattcggt tggtattccc tttcaagcgt attggtaaac
     9061 gatgcttggg gcccgattta cggcagtatt gcctttttta acgacatctc gcgtgaaatt
     9121 ttatgcctgt ttttagtacc actgtttatg cgtcaatttc cgtccaccgc agtcggcatt
     9181 ggcggagcga cttcattaga ttgtatgctc cctattattc aaa
//
LOCUS       DANKBV010000088         9071 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.088, whole genome shotgun sequence.
ACCESSION   DANKBV010000088 DANKBV010000000
VERSION     DANKBV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.088"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(21..650)
                     /locus_tag="PWY43_001713"
     CDS             complement(21..650)
                     /locus_tag="PWY43_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDL2136577.1"
                     /translation="MDIFIQGFIVCFGLIVSIGAQNAFLLKQGILKQHIFWIALLCFL
                     GDVFLMTIGVLGLGTLIAELPVVSFIISLLGAIFLLSYGSRSFISVFKSGEYLVASGE
                     NATSLKKALLITFAITFLNPHVYIDTVVIVGSIGGKLDFNGKIYFLAGALLCSFIWFF
                     GIGYGASLLSPYFAKRRTWQILDAVTGLIMYFIAFSLLIYTYQLALQIF"
     gene            754..1401
                     /gene="udk"
                     /locus_tag="PWY43_001714"
     CDS             754..1401
                     /gene="udk"
                     /locus_tag="PWY43_001714"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL2136578.1"
                     /translation="MTDTSQTSSCVVIAIAGASASGKSLIASTIYKELKNELGTDNIG
                     IISEDAYYRDQSHLTMEERIKTNYDHPNSMDHHLLVEHLRQLKQGNAIEIPEYDYTEH
                     NRKTTTKHFEPKKIIILEGILLLTDENIRNEINVSIFVDAPLDICFIRRLQRDMVERG
                     RSMESVISQYRKTVRPMFLQFIEPSKQYADIIVPKGGKNRIAIDILKAQIKQLLK"
     gene            1416..1997
                     /gene="dcd"
                     /locus_tag="PWY43_001715"
     CDS             1416..1997
                     /gene="dcd"
                     /locus_tag="PWY43_001715"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDL2136579.1"
                     /translation="MRLCDTDIKRYLDEGIIAITPRPSDDKISGATADVRLGNSFRVF
                     REHKTPYIDLSGPREEVAAQLNKVMSDEIIIGDDEAFFLHPGDLALATTLESVSLPAN
                     IVGWLDGRSSLARLGLMVHVTAHRIDPGWEGKIVLEFFNAGKLPLALRPNMAIGALSF
                     EILSGDAAKPYNARKDAKYKNQQSAISSRINQD"
     gene            2075..2230
                     /locus_tag="PWY43_001716"
     CDS             2075..2230
                     /locus_tag="PWY43_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDL2136580.1"
                     /translation="MLHYAVVFFVIAIIAALLGFGGIAGSATEIAKILFFVFLILTVI
                     SFFFKKK"
     gene            complement(2359..2961)
                     /locus_tag="PWY43_001717"
     CDS             complement(2359..2961)
                     /locus_tag="PWY43_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL2136581.1"
                     /translation="METLIEFFSSYGYWAVFLVLLACGFGVPIPEDITLVSGGVISGL
                     GYTNVHWMLVVSMLGVLVGDSTMYWLGRIYGEKILKFPLIRNVATPERFATVQERFEK
                     QGWKLLFMARFLPGLRAVVYLVSGITRKVSFVKFLLVDFCAAIISVPIWVYLGDYGAS
                     NLDWLKEQVANGQHLIFAILGIVILFLGWKWYKNKKQYKK"
     gene            complement(2963..3982)
                     /gene="purR"
                     /locus_tag="PWY43_001718"
     CDS             complement(2963..3982)
                     /gene="purR"
                     /locus_tag="PWY43_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDL2136582.1"
                     /translation="MATIKDVAKYAGVSTTTVSHVINKTRFVAEDTTKAVWEAIKALN
                     YSPSAVARSLKINTTKSIGMIITTSESPFFAEIVLAVEEFCYSKGYSLFLCNTQNKLE
                     KIQNHLDMLIKKRVDGILVMCSEYTANSFDIFENSNIPMVIMDWGRSDERSDIILDHG
                     VEGGYIATQHLIENGHQDIAVITGNRDKEIARTRFEGFKKALSEAGLSIREEWIQEGD
                     FEPEGGYECMNNLLKSSEKLPTAVFCFNDSMALGAISAITEKGLNVPQDISVIGYDNV
                     HSSRFYAPPLTTIHQSKSRLGSAALELLLERIEDNENAKEPKVLEFFPELVKRKSVRN
                     LNIKE"
     gene            complement(4118..4846)
                     /gene="cmoA"
                     /locus_tag="PWY43_001719"
     CDS             complement(4118..4846)
                     /gene="cmoA"
                     /locus_tag="PWY43_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDL2136583.1"
                     /translation="MQRDTLFSAPIEKLGDFTFDESVAEVFSDMIQRSVPGYSNIITA
                     IGMLAERFVTDNSNVYDLGCSRGAGILSIRRNVNAKNVQIIGVDNSQPMVERCKTHLS
                     AYHSEIPVTILLDDIRHIEIKNASMVVLNFTLQFLPREDRLALLQKIYQGLNANGILV
                     LSEKFTFESEEMNELLIDLHHTFKRANGYSELEVSQKRNALENVMRTDSIETHKKRLQ
                     EAGFTQVELWFQCFNFGSMIAIKA"
     gene            complement(4856..5638)
                     /locus_tag="PWY43_001720"
     CDS             complement(4856..5638)
                     /locus_tag="PWY43_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2136584.1"
                     /translation="MTEKSQLNFNYQPSNNHANAQTMIFLHGLFGDLNNLGAIAKPFA
                     ERYSILMVDLRNHGRSFHSDEMNYALIAQDLAQLLTELNIQNAIVVGHSMGGKSAMAL
                     AAFAPELVEKLVVIDIAPVTYTQNRHQQVMAALFAVKEAKPQNRQQAKAIMAEYIRDE
                     GVQQFMLKSFDAQSEQSFRFNLQALKDNYQNVMGWQQVFVDKPTLFIKGALSDYIQAE
                     HTQTILAQFPQAKSFIVANADHWVHAEKTETVVRAINKFLEK"
     gene            5757..6323
                     /gene="seqA"
                     /locus_tag="PWY43_001721"
     CDS             5757..6323
                     /gene="seqA"
                     /locus_tag="PWY43_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDL2136585.1"
                     /translation="MKRIEIDDELYQYIASRTQSIGETASDILRRLLRLPQSPQPFVL
                     VQEHMINELKELVKTPSRATKKNESNAEKTVEKLENILNSEHFMNENKNVVRFTMLLA
                     ALYRANPEAFAKATEKVRGNERIYFSQSEAEILATGSGVKAKQIPDSPFWVITNNNTA
                     RKGLILKGVMESMKVPSQLIERIQALFI"
     gene            6333..7751
                     /gene="menE"
                     /locus_tag="PWY43_001722"
     CDS             6333..7751
                     /gene="menE"
                     /locus_tag="PWY43_001722"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDL2136586.1"
                     /translation="MLINSTRFPTQFWAAHSAQNVAIIWEKGTSDYLNHLPSNITWYS
                     LNQLIAQAIFVLQQQGATPHSLIAYCGSHRLIGLLTYCASLALGAKILMLNPAQKASQ
                     RQAVLDDNGVDLIINDADFAKFSAKTTACNAFPTIDFEKPATLTLTSGSSGKPKAVVH
                     SIAAHLYSAEGVCELMAFEQPNSWLLSLPLFHVSGQGIIWRWLFKGATLYVHEGKSDF
                     FLSAQKVSHLSLVPTQLQRYLNQLDSMVSQKCLLGGSMLPAELIAQAQQSGMTTFSGY
                     GMTEMASTICAVENDLNSVGFPLKHRELKLENGEIWVKGKSLGLGYWQKNGQIRPLVN
                     TQGWFATKDRGEWNHKKQLVIKGRLDNMFISGGENIQPEEIEQLIFRSQLVEQIIVLP
                     IEDKQFGHRPVAFLQFKQSDSKNELHKLKIWLSDKLERFKQPVAYYLLDIEKYQKQGL
                     IKLSRTQLQQDLKLKVLKEIYV"
     gene            7744..>9071
                     /locus_tag="PWY43_001723"
     CDS             7744..>9071
                     /locus_tag="PWY43_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL2136587.1"
                     /translation="MFRPILKTFVLSLGLMLNSYALAELPTQQAVQTQLDSAKKLDQA
                     DADNKALVQTLEDTLAFLAQIDKQKADNQALNKSIQDSQKALKTSQHNLEKLKEVTIA
                     SVDNLTQKATTDLQKELTSTQSQLETVQHDLSIINNNLVAQNTAPDKAQTALTEMVKR
                     KQEISSQLSATNIKNELQTKLEVELELIELKNTYNQILLGGSEDLSALYSAQLDEKKQ
                     EQQNLQAEIANLQTAINNKIVEESQNKLDQAQAVQNQQNNATTNPVILRELDINTKVT
                     QELLKQTKDMTQLSQDNLRIKSVLDNLQQTQRNIEEQISSLQGTLVLSRIINKQKQSL
                     PQDEMISGLSKQIADLRVRVFDITEFKDSFADINAYISRIEQDEKTTFTSKEKEQLSK
                     ILQERSDTLTEMIKSLNNQLNLLINIELNQQQAQTISDALQQKLQQQSFWV"
BASE COUNT         3040 a         1808 c         1558 g         2665 t
ORIGIN      
        1 atgaaaaaat ttaaactcca ctagaaaatt tgcagagcaa gttgataagt ataaatcagt
       61 aaactaaaag cgataaagta cataattagc ccggtaacag catctaaaat ttgccaagtt
      121 cttcgctttg caaaataagg ggaaagtaag cttgctccat aacctatgcc aaaaaaccaa
      181 ataaatgagc aaagtaatgc acctgctaaa aagtatattt taccgttaaa atctaatttc
      241 ccaccgatac tgcctacaat cacaaccgta tcaatataaa cgtgaggatt taagaatgta
      301 atcgcaaagg taatcagtaa agcctttttt aagctcgttg cattctctcc acttgccaca
      361 aggtattcac cacttttaaa tacgctgata aatgaacggc taccgtagct taatagaaaa
      421 atggccccta aaagcgaaat aatgaaactt acaacaggca attccgcaat taacgtgcct
      481 aagcctagca cacctatcgt cattaaaaat acatcaccta aaaaacagag caatgcaatc
      541 caaaatatgt gttgttttaa aatcccttgc tttaataaaa aggcattttg tgcgccaata
      601 gagacaatta agccaaaaca cactataaat ccttgaatga aaatatccat tttttctcca
      661 aaatcgcata acaagcggtc aattatacag aaaattttgc aaaacttcgg tataatttat
      721 cgaatactct tttgactgaa taggaaattt tttatgactg atactagcca gacttcatct
      781 tgcgttgtca ttgcgattgc cggcgcatct gcctcaggta agagcctgat tgcctcaacc
      841 atctataaag aactcaaaaa tgaattggga acagataata tcggtattat ttccgaagat
      901 gcttattata gagatcaatc tcatctaaca atggaagaac ggattaaaac caattacgat
      961 caccctaatt caatggatca tcatttattg gttgaacatt tacgccaatt aaaacaaggt
     1021 aatgcgattg aaattccaga atatgactat accgaacata atcgtaaaac gaccacgaag
     1081 cactttgaac caaagaaaat cattatttta gaaggtattt tattgctgac agatgagaat
     1141 attcgcaatg aaattaatgt ttctattttc gtagatgctc ctctggacat ctgctttatc
     1201 cgccgattac aacgtgatat ggtggaacgt ggacgttcga tggaatcagt gattagtcaa
     1261 tatcgcaaaa ccgttcgccc aatgttctta caatttatcg aaccctctaa acaatatgct
     1321 gatattattg tgccaaaagg tggcaaaaac cgtattgcca ttgatattct taaagcacaa
     1381 attaagcaat tactaaaata aaataggagc taaatatgcg tttatgtgat acagatatta
     1441 aacgttactt agatgaaggt attattgcca taacgcctcg accttcagac gataaaattt
     1501 ctggcgccac tgcagatgtg cgtttaggta attcgtttcg tgtatttcgt gaacataaaa
     1561 cgccctatat tgatttaagt ggccctcgag aagaagttgc cgcccagtta aataaagtga
     1621 tgagtgatga aattatcatc ggtgatgatg aagcattttt ccttcatccc ggtgaccttg
     1681 cactcgcaac aacccttgaa tcagtttcct taccagccaa tattgttgga tggttagatg
     1741 gtcgttcgtc acttgctaga ttaggcttaa tggtacacgt taccgcccac agaatcgatc
     1801 cgggttggga aggcaaaatt gtattggaat tttttaatgc tggaaaatta cccctagctt
     1861 tacgccctaa tatggcaatt ggcgcattaa gctttgagat tttaagtggt gatgctgcaa
     1921 aaccctataa tgcgcgaaaa gatgcaaaat ataagaacca gcaaagtgcc atttcaagtc
     1981 ggattaatca agattaatat agtacatgcc ttgcaatcat acattaacta tgttagtttt
     2041 ttaatcagtt tattacacag taaggagtat aactatgtta cattatgcag tcgtattttt
     2101 tgtcatcgct ataatcgcag cgctattagg atttggtggt attgctggaa gtgctactga
     2161 aattgccaaa attctctttt ttgtcttttt aatacttact gttatttcgt tcttcttcaa
     2221 gaaaaagtga cacagttaaa cctaattttt taagttaaaa tatagttcaa aaaaacagtt
     2281 ggtaatggat atttataatt attcttacca actgttttgc tattatatct ttttaagatg
     2341 aaatgctcgt cttttgtttc actttttata ctgcttttta tttttatacc atttccagcc
     2401 taaaaataga ataacaatgc ctaaaatggc aaaaatgagg tgctgcccat tagctacttg
     2461 ttcttttagc caatccaaat tagatgcccc atagtcgcct aaataaaccc aaattggcac
     2521 agagataata gcggcacaga aatcaactaa taaaaatttt acgaagctga cttttctggt
     2581 aatacctgaa actaagtaaa ccaccgcgcg caagccaggt aaaaaacgag ccataaaaag
     2641 tagtttccat ccttgttttt caaaacgttc ttgcacggtt gcaaaacgtt caggtgtcgc
     2701 aacattcctg attaatggaa atttgagaat tttttcgcca tagattctac ctagccagta
     2761 cattgtgcta tcgccaacta atactcctaa catactaaca accagcatcc aatgcacatt
     2821 ggtgtagcct aatcctgaaa taacaccacc ggatactaac gtaatatctt caggaatcgg
     2881 cacaccgaaa ccacaggcaa gtagcacaag aaaaacagcc caataaccat agctactaaa
     2941 aaattcaatc aatgtttcca tattactcct taatgtttaa attccgaacc gatttacgtt
     3001 tgaccaattc aggaaagaac tctaatactt taggctcttt tgcgttttcg ttatcttcaa
     3061 tacgttctaa taataattct aatgctgccg agcctaagcg agattttgac tgatggattg
     3121 tcgtaagagg cggtgcataa aagcgtgagc tatgcacgtt atcatacccg atcactgaaa
     3181 tatcttgcgg cacattcagc cctttttcgg taatagctga aattgcccct agtgccattg
     3241 agtcattaaa acaaaaaacc gcggttggta atttttcact gctcttaagc aaattattca
     3301 tacactcata ccccccctca ggttcaaaat cgccttcttg aatccattct tcacgaatag
     3361 acaaacccgc ctcagatagc gcttttttaa aaccttcaaa acgggttctt gcaatttctt
     3421 tatctctatt gccggtaatt acagcaatat cttgatgccc gttttcaatt aaatgctgag
     3481 tcgcaatata gccaccttca acaccgtgat ctaaaataat atcactgcgt tcatcacttc
     3541 gtccccaatc cataatcacc attgggatat tgctgttttc aaagatatca aatgaattag
     3601 ctgtatattc ggagcacatc accaaaatgc catcaacccg ttttttgatg agcatatcta
     3661 aatgattttg aattttctca agtttgtttt gagtattgca caaaaatagt gaataccctt
     3721 tgctatagca aaattcctct accgctaaca caatttcagc gaaaaacggt gattcgctgg
     3781 ttgtaatgat cataccgata gatttagtcg tattaatttt caaactccgc gctacagcac
     3841 taggagaata gttcagcgct ttaatagcct cccaaactgc ttttgtagtg tcctctgcta
     3901 caaagcgggt tttgtttata acgtgagaaa ccgttgtagt cgatacacca gcatattttg
     3961 cgacatcttt tatcgttgcc ataccttttt cctcctaaat aagtagtggt gcattataac
     4021 tgattctaac tgacttttat tataaagaaa tagatagaat gcaagcggtt gctttttatc
     4081 aagaatttgc aaaaaatttt acaaatatca ccgcttgtta ggctttaatt gcaatcattg
     4141 agccaaaatt aaagcattga aaccaaagtt caacttgtgt aaaaccagct tcttgcagac
     4201 gttttttatg ggtttcaata ctatctgttc gcatcacatt ttccaaagca ttacgctttt
     4261 ggctgacttc caattcactg tagccatttg ctcgtttgaa tgtgtgatga aggtcgatca
     4321 atagctcgtt catttcctca ctctcaaagg taaatttttc cgataacact aaaatgccat
     4381 tggcatttaa tccttgataa atcttctgta ataatgctag gcgatcttct cttggtaaaa
     4441 attgcaaggt gaaattaagt accaccattg aagcattttt aatttcaatg tggcgaatat
     4501 catcaagtaa aatcgtcacc gggatctcag agtgataagc acttaaatgg gttttacaac
     4561 gctcaaccat cggctgagaa ttatctaccc cgataatctg cacattcttc gcattcacat
     4621 tacggcgaat agataaaatc cctgcgcctc gtgaacaacc taaatcataa acatttgaat
     4681 tatctgtgac aaaacgctcc gccaacattc caatagcagt aataatattg gaatagcccg
     4741 gcacggagcg ttggatcata tccgaaaaca cttcagcaac cgattcatca aaagtaaaat
     4801 cgcccaattt ctcaatcgga gcggaaaata atgtatctct ttgcattttt ctcccttatt
     4861 tttctaaaaa tttattgatt gctctcacaa cagtttctgt tttttctgcg tgaacccagt
     4921 gatcagcgtt tgccacaatg aaagatttgg cctgcggaaa ttgtgccaaa atcgtttgtg
     4981 tatgctctgc ttgaatataa tccgataatg cacctttaat aaacaaggtc ggtttatcca
     5041 caaatacctg ctgccacccc atcacattct gataattatc ttttagcgcc tgtaaattaa
     5101 aacggaaaga ttgctcgctt tgggcatcaa aagatttcaa cataaactgt tgcacgcctt
     5161 catctcgaat atattcagcc attatcgctt tagcttgctg acgattctgg ggtttagctt
     5221 ctttcaccgc aaataaggct gccataacct gctgatgccg attttgggta taagtgaccg
     5281 gggcaatatc aatcactacc aatttctcca ccagttcagg agcaaatgca gccaatgcca
     5341 tcgcactttt tccgcccatt gaatgcccaa ccacaatagc attttggata tttaattctg
     5401 tgagtaattg tgctaaatct tgtgcaatca aagcataatt catttcgtct gaatgaaaag
     5461 atcgcccgtg atttctgaga tcaaccatta aaatggagta acgttctgcg aacggttttg
     5521 ctattgcgcc taggttattt aaatctccaa acaatccatg taagaagatc atcgtctgag
     5581 cattagcatg attattcgaa ggttggtaat taaagtttaa ctgcgatttt tctgtcatat
     5641 aaatgtcatt gttcatataa attttcagcg atgatcctct ataaaaaaca atagaaatgc
     5701 gtataatagc aaacactatt aacgcattta atacccgcag atatttgagg atcccgatga
     5761 aacgaatcga aattgatgat gaattatatc agtatattgc aagccgtaca caatctattg
     5821 gtgaaactgc gtcagatata ttacgccgtc tattacgcct gccacaatcg cctcagcctt
     5881 ttgttttagt acaagaacac atgatcaacg agcttaaaga gcttgtcaaa acgccttctc
     5941 gtgctacgaa aaaaaatgaa tccaatgcag aaaaaaccgt tgaaaaattg gaaaacattt
     6001 taaattccga gcattttatg aacgaaaata aaaatgtagt gcgttttact atgctattag
     6061 ccgcattata tcgagcaaat ccggaggctt ttgctaaagc aaccgaaaaa gtgcgtggta
     6121 atgaacgtat ctacttttcg caaagtgaag ccgaaatttt agctactggg agtggagtaa
     6181 aagccaagca gatcccagat tcaccgtttt gggtaattac caataataat accgcacgca
     6241 agggacttat tctcaaaggc gtaatggaat ccatgaaagt accaagccag ttaatcgaac
     6301 gcattcaagc gctttttatc taattaattg aaatgctcat caattctact cgctttccta
     6361 cccaattttg ggctgctcat tcagcccaaa atgtggcgat tatttgggaa aaaggaacat
     6421 cagactatct taaccacctt ccatcaaaca tcacttggta tagtttgaat cagttgattg
     6481 cgcaagctat tttcgtgttg caacaacaag gtgctactcc gcacagtttg attgcctatt
     6541 gtggttctca tcggctcatt ggcttgctca cttattgtgc tagccttgct ttgggggcta
     6601 agattctaat gcttaaccct gctcaaaaag caagtcaacg acaagcagtc ttagatgaca
     6661 atggtgttga tctcattatt aatgatgccg actttgcaaa attttcagca aaaacgaccg
     6721 cttgtaatgc cttccctacg attgattttg aaaaaccagc taccctaacc ctaacctcag
     6781 gctcaagcgg taaacctaaa gcagtggtgc attctatagc ggcccatctc tatagtgccg
     6841 aaggagtatg tgaattaatg gcatttgagc agccaaactc ttggctactg agcttgcctc
     6901 tgtttcacgt ttccggacaa ggcattattt ggcgttggtt atttaaaggt gcaacactgt
     6961 atgttcacga aggcaaaagt gatttttttc tttcagcaca aaaagtgagc catctctctt
     7021 tagtccccac ccaattacaa cgctatttaa accaattaga ttctatggtc agccaaaaat
     7081 gtttactggg tggatctatg cttccagccg aattgattgc tcaagcacaa caatctggga
     7141 tgactacctt ttcaggctac ggaatgactg agatggcatc aaccatttgt gcagtagaaa
     7201 atgatttaaa cagtgttggt tttccgttaa aacatcggga actaaaatta gaaaatggtg
     7261 aaatttgggt aaaaggcaaa tcactcggct taggctattg gcaaaaaaat gggcaaattc
     7321 gaccgcttgt aaacacacaa ggctggtttg ccactaaaga tcgtggcgaa tggaatcaca
     7381 aaaaacagct tgtaataaaa ggacgattgg ataatatgtt tatttcagga ggggaaaata
     7441 tccaacctga ggaaattgaa caactgattt ttcgttctca gcttgtcgaa cagattatcg
     7501 tgttgccgat tgaagataag caatttggac atcgaccagt ggcgttctta caattcaagc
     7561 aatctgattc aaaaaatgaa ctccataaat taaaaatttg gctgtctgat aaattagaaa
     7621 gattcaaaca gcctgttgcc tattatttac tggatataga aaaataccaa aaacaaggct
     7681 taattaaact atcaagaaca caattacaac aagatttaaa actcaaagta ttaaaggaaa
     7741 tctatgttta gaccgatttt aaaaacattt gtgcttagtt taggcttaat gctaaatagt
     7801 tatgcattag ctgaattgcc aacccaacag gcagtgcaaa cgcagttaga tagcgccaaa
     7861 aaattagatc aagccgatgc ggataataag gcattggttc aaacacttga agatacgctt
     7921 gctttcttag cccaaattga taaacaaaaa gcagataatc aggcattgaa taaatctatt
     7981 caagattccc aaaaagcatt aaaaacaagc caacataatc ttgaaaaatt aaaagaggtg
     8041 actattgcaa gtgtcgataa tttaacacaa aaagcgacaa cagatttaca aaaagagtta
     8101 acctctacac aatctcagct ggaaacagta caacacgatt taagtattat taataataat
     8161 ttagttgcac aaaataccgc accggataag gctcaaacgg ccttaaccga gatggttaaa
     8221 cgtaaacagg aaattagtag ccaactttca gcgactaaca ttaaaaatga actacagaca
     8281 aagttagaag tagaattaga attaatagaa ttaaaaaaca cttacaatca aatattactt
     8341 ggtggcagcg aggatttatc tgcgctttac tctgctcaat tagatgaaaa aaagcaagag
     8401 cagcaaaatc tacaagcaga aattgcaaac ctgcagactg ctatcaacaa taaaattgta
     8461 gaagagtctc agaacaagct agatcaagca caagcggttc aaaatcagca aaataatgca
     8521 acgacgaacc cagttatctt gcgtgaattg gatataaata ccaaagtcac acaagagttg
     8581 ctaaaacaga ccaaagatat gacgcagtta tcacaagata atttgcgaat caaaagtgtg
     8641 ctagataatt tacagcaaac ccaacgtaat attgaagaac aaatcagttc tctgcaaggt
     8701 acgttggtgc tttctcgtat tattaataag caaaaacaat cattaccaca agatgaaatg
     8761 atttccggac tatcaaaaca aattgcagat ttacgtgtac gtgtttttga tatcactgaa
     8821 tttaaagact cttttgcgga cataaatgct tatatcagcc gcatagagca agatgaaaaa
     8881 acaaccttta cgagtaaaga gaaagaacaa ctgagtaaaa ttttacagga acgttctgac
     8941 actttaactg agatgattaa atcattaaat aatcagctca atttattaat caatattgaa
     9001 ctaaaccagc aacaagcgca gaccattagt gatgcgttac agcaaaaact gcaacagcaa
     9061 agcttctggg t
//
LOCUS       DANKBV010000089         9026 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.089, whole genome shotgun sequence.
ACCESSION   DANKBV010000089 DANKBV010000000
VERSION     DANKBV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.089"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            61..771
                     /locus_tag="PWY43_001724"
     CDS             61..771
                     /locus_tag="PWY43_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136588.1"
                     /translation="MTNQKFINSSLTLAFFTLISFSTAYCYAWGIAAFHGYPWWHVEV
                     GNAGIARALAYVFGTFLTIFLFYLIGYALVNKVFKLHYFKYLGWLRVSVLVTIFSLPV
                     MISFYLFIGKVPMYLHLLYLGTTTLSVLLFHKHWDHRVFHLDIQKMLSEERFGFFYVF
                     IFIYFSLLSLSIGYIRPELRTTYDYLEIENKRYYVLSIHRNNIYVLGEKTKDNDEFLF
                     FNQDTLKYYRINITKMPN"
     gene            complement(825..1970)
                     /gene="lldD"
                     /locus_tag="PWY43_001725"
     CDS             complement(825..1970)
                     /gene="lldD"
                     /locus_tag="PWY43_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HDL2136589.1"
                     /translation="MIISSANDYRRAAKRRVPPFMFHYADGGSYAEQTLRRNVSDLED
                     IALRQRVLKDMSQLDTGIELFGEKLSMPVTLAPVGALGMYARRGEVQAGKAADNKGIP
                     FTLSTVSICPIEEVAPAIKRPMWFQLYVLKDRGFMKNALERAKAAGCSTLVFTVDMPT
                     PGARYRDMHSGMSGPYKDIRRVLQAMVHPFWAWDVGIHGKPHTLGNVSNYMGKPIDLN
                     NYIGWLTDNFDPSISWKDLEWIREFWDGPMVIKGILDPEDAKDAVRFGADGIIVSNHG
                     GRQLDGVLSSAKALPSIADAVKGEIKILADSGIRNGLDVVRMLALGADCTMIGRSFVY
                     ALSAAGQAGVENLLDIFLKEMKVAMTLTSNAKISDIGRDALVDLSNL"
     gene            2582..3226
                     /gene="rppH"
                     /locus_tag="PWY43_001726"
     CDS             2582..3226
                     /gene="rppH"
                     /locus_tag="PWY43_001726"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDL2136590.1"
                     /translation="MIDFDGYRPNVGIVICNKHGQVLWAKRFGQNSWQFPQGGINEGE
                     NIEAAMYRELYEEVGLSKKDVRLLWASKYWLKYKLPKRLVRNEGSGPVCIGQKQRWFL
                     LQLIGDESLINLKTTKNPEFDGWRWVSFWYPVRQVVSFKRDVYRKAMKEFAQVLQNNI
                     QKATDDKTGEKPRIERGETSPHTYKKTSQDSAKAYKREARKPYRPWHQHNQKGK"
     gene            3228..4016
                     /locus_tag="PWY43_001727"
     CDS             3228..4016
                     /locus_tag="PWY43_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2136591.1"
                     /translation="MTILLSCLFLGVFVGFLAGLFGIGGGLIIVPSLVFLLPMAGVPP
                     EYIMSTALGTSFSTIIITAFASAQRHHKLGNVDIQTSKYFIPALMVSVFLSGLVVSRL
                     DPQLMSRIFAVMVLYLAGKMIFSIKRAPKNKPLTVQTTIIGGSIIGALASMAGIAGGA
                     FIVPFLNDRGLEMKRAIGTSSFCGAFLGLSGTISFIVSGWGLEGMPDYSLGYVYLPAL
                     FGITATSFFTSKMGASAANALPVPMLKKAFAVLLVCIAINMFLK"
     gene            4024..4821
                     /gene="lgt"
                     /locus_tag="PWY43_001728"
     CDS             4024..4821
                     /gene="lgt"
                     /locus_tag="PWY43_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2136592.1"
                     /translation="MNEQFIQFPQISPIVFEIGPIALRWYGLMYLAGFAFAYWLGMRR
                     AKNSNGVWTTEQVDSLVYTCFWGVVLGGRIGDVFFYSFDRFLQDPLYLFRIWEGGMSF
                     HGGLIGVIVAMIWVSYRQKRNFWQTADFIAPLVPFGLGMGRIGNFINDELWGRVTDVP
                     WAVLFPSGGYLPRHPSQLYEAFLEGVVLLFILNWFIQKPRPAGAVSGLFLIGYGTFRF
                     IVEYFREIDHNVNTTADLITRGQLLSLPMIIGSILIMAWAYKVNKVK"
     gene            4908..5576
                     /locus_tag="PWY43_001729"
     CDS             4908..5576
                     /locus_tag="PWY43_001729"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDL2136593.1"
                     /translation="MSKKYKVIGFDLDGTLINTLPDLTLVANSMFLEAGLPTVTQEKV
                     LTWIGKGADIFFQNAVAHTGRIFQADELVKMRVSFDKYYATYVCEESTLFPNVKETLE
                     ILKANDYPLVVITNKPTKLVEPVLSAFGIYHLFDETLGGQSLPKIKPHPDPMFFICEK
                     FGITPSELLFVGDSENDVIAAKAAGCDVVGLTYGYNYNVPIEQSNPTFVTSEFKDVLK
                     IVGL"
     gene            5646..5906
                     /locus_tag="PWY43_001730"
     CDS             5646..5906
                     /locus_tag="PWY43_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL2136594.1"
                     /translation="MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVI
                     NPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPAIGKEIL"
     gene            5903..6235
                     /locus_tag="PWY43_001731"
     CDS             5903..6235
                     /locus_tag="PWY43_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2136595.1"
                     /translation="MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVP
                     ITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTVDIAQRHGKFIEALPESLLDEIM
                     ARVSVIFE"
     gene            6362..7393
                     /gene="trpS"
                     /locus_tag="PWY43_001732"
     CDS             6362..7393
                     /gene="trpS"
                     /locus_tag="PWY43_001732"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL2136596.1"
                     /translation="MSKPIVFSGVQPSGELTIGNYLGALRNWVKMQDDYDCVYCIVDQ
                     HAITVRQDPESLRKSTLDVLALYLACGIDPNKSTIFIQSHVPEHAQLAWVLNCYTYFG
                     EMGRMTQFKDKSARYEENVNVGLFTYPVLMAADILLYQANQVPVGDDQKQHLEITRDI
                     ANRFNALYGKKDSEGNLIEPVFAVPEVFIAKSGARVMSLLEPTKKMSKSDDNRNNVIG
                     LLEDPKAVAKKIKRAMTDGDEPPVVRYDVQNKAGVSNLLDILSAITGKTIAELETEFE
                     GKMYGHLKTEVADKVSELLTNLQERYHHYRQDEALLEKIYREGAEKARAQAKATLDKV
                     YELVGFVRY"
     gene            complement(7457..8212)
                     /locus_tag="PWY43_001733"
     CDS             complement(7457..8212)
                     /locus_tag="PWY43_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDL2136597.1"
                     /translation="MKFIKKLSYSVFLATVITACATTESINQEAAQSYAQVKTQARQQ
                     NAIDNSSATAKRIHTVFHKMKPYAERANQTGVPFHWEITVLKSDELNAWAMPGGKMAF
                     YTGLVERLNLSNDEIAVVMGHEMAHALKEHGKSDRTVSAITSIVGAIADVAVTASTGV
                     STEGLLSTGVDLIATKPFSRSQETEADEVGLILMAESGYNPSAAPNVWVKMSKANGDS
                     GLSIFSTHPSNADRQENLARLVPEAMKIYNARK"
     gene            complement(8268..8795)
                     /gene="ppa"
                     /locus_tag="PWY43_001734"
     CDS             complement(8268..8795)
                     /gene="ppa"
                     /locus_tag="PWY43_001734"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDL2136598.1"
                     /translation="MGLELVPAGKELPDDIYVVIEIPANSDPIKYEVDKETGALFVDR
                     FMATAMFYPANYGYVNNTLSSDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGG
                     DAKVVAVPHTKLSKEYDHIKDVGDLPALLKAQIQHFFESYKALEAGKWVKVEGWGDVN
                     EARQEILESFERAKK"
BASE COUNT         2542 a         1943 c         1889 g         2652 t
ORIGIN      
        1 aactcagtct gctttcttgt gtttcatctt taactaagta ggtgcaatct atttactgtt
       61 atgactaacc agaaattcat caactcttct cttactctcg ctttctttac tttaatctct
      121 ttttctaccg cttactgtta tgcttggggt attgctgctt ttcatggcta cccgtggtgg
      181 catgttgagg ttggtaatgc cggtatagct cgcgcactag cctacgtttt tggcacattt
      241 ttaactatct ttctttttta tttgatagga tacgctttag ttaataaagt ttttaagctg
      301 cattacttta aataccttgg ctggctacgg gtgagtgttc ttgttactat ttttagcttg
      361 ccagtaatga tcagtttcta tctgtttatt ggcaaagtgc caatgtattt acacctactc
      421 tatctaggga caactaccct atcagtatta ttatttcaca aacattggga ccaccgcgta
      481 ttccacttag atattcaaaa aatgttaagc gaagaacgct tcggtttttt ctatgttttt
      541 attttcatct attttagctt actttctttg agtattggct acattcgccc tgagctgaga
      601 acaacgtatg attatcttga aattgaaaat aagcgatatt atgtcttatc tattcatcgc
      661 aataacattt atgtgctggg cgaaaaaact aaagataacg atgaatttct ctttttcaac
      721 caagatacat tgaaatatta ccgcattaat attaccaaaa tgccaaatta gcctatctaa
      781 aataacaatc cgcacctaaa acgtgcggat tgtggctatt tgagctaaag attacttaaa
      841 tctaccaaag catctctgcc gatgtcgcta attttagcat tgctggtaag tgtcatcgcc
      901 actttcattt ctttaaggaa gatgtcgagc aggttttcca caccggcttg accggcggcg
      961 ctaagagcgt aaacaaagga acgcccgatc atcgtacaat ctgcgccgag agccagcatt
     1021 ctcaccacat ctaagccgtt gcggatacca gagtcggcaa ggattttgat ctcccctttt
     1081 actgcatcgg caatgcttgg cagggctttg gcagaagaaa gtaccccatc taactgacga
     1141 ccaccgtggt tggagacgat gataccgtcc gcaccaaatc ttacggcatc tttagcatct
     1201 tctgggtcga gaatgccttt gatcaccatc gggccgtccc aaaattcacg aatccactct
     1261 aaatctttcc acgaaattga tgggtcgaaa ttatcggtca gccagccgat gtagttgttc
     1321 agatcaatcg gtttgcccat atagtttgac acattgccta acgtgtgggg tttaccgtga
     1381 atgcccacgt cccacgccca aaacgggtga accattgcct gcaacacacg gcgaatgtct
     1441 ttgtatgggc cgctcattcc ggagtgcata tcacgataac gtgcgccggg ggtcggcata
     1501 tctacggtaa acactagcgt ggaacaaccg gcggctttgg ctcgttccaa tgcatttttc
     1561 ataaagcccc gatcttttaa cacatagagc tgaaaccaca tcgggcgttt gattgccggt
     1621 gcaacctctt caatcgggca aatagagaca gtggaaaggg taaacggaat acctttgtta
     1681 tctgcggctt tgcccgcttg cacttcgcca cggcgggcat acattcctaa tgcaccgaca
     1741 ggggcgaggg tcaccggcat agacagtttt tcgccgaaca gctcgatgcc ggtgtctagt
     1801 tgcgacatat cttttaacac acgttgacgc agggcgatgt cttccaaatc gctcacatta
     1861 cgacgtaagg tctgctcggc gtaagaaccg ccatccgcat aatggaacat aaaaggggga
     1921 acacgacgtt ttgccgcacg gcggtaatcg tttgcagatg aaatgatcat ttaaaacctc
     1981 ataaatcatt aagaaaaaag tgtggctatt ctactttaaa ataaaaataa gaattactat
     2041 tatttgcaaa atatgagaaa tagatcacat ttttgttaca aactaattag cataaaaata
     2101 caattgcaca aaaagcaacc aacaaaacaa ttgattattc acagattgaa aagatgacac
     2161 taactatttg atttaattat aatttatgga aatagcaaaa ggctgatttg agttattcac
     2221 gcctaatttg aaaaactatc acatctttat ccacaaatgg tggggataac tctgctttat
     2281 ttacgaagca aaaattgccc tgcttattca ttttaaaatt taaacttatt ttgacgagaa
     2341 aaaacgcaac aacttgatct ttatccagat cattgcttaa gaaaaagatc gcatttttag
     2401 atctgattaa ttgctctgtt ttacaacgtt ccgcctgttt atcctcaact tataccactt
     2461 atattcaata gttatccaca aagttattca caagcggtta tattttatgg tttttttgca
     2521 aatcaaccca aagtatgaaa caattctccc attctagaat ttttatcaat taggtggtcg
     2581 agtgatcgat ttcgatggct accgcccaaa tgttggaatc gttatctgta ataagcacgg
     2641 gcaagttctg tgggcaaaac ggtttggtca aaactcttgg caatttccgc aaggtggaat
     2701 taatgagggg gaaaacattg aagcagcaat gtaccgtgag ctttatgaag aggtcggctt
     2761 aagtaaaaaa gatgtacgat tgctttgggc gtctaaatat tggctaaaat ataaactgcc
     2821 aaaacgcttg gtacgtaatg aaggctctgg tcctgtctgt atcgggcaaa aacagcgttg
     2881 gtttctactg caattaatcg gtgatgaaag cctgattaat ttgaaaacca cgaaaaatcc
     2941 ggagtttgac ggctggcgtt gggtcagctt ttggtatcca gtccgccaag tggtctcttt
     3001 taaacgagat gtttatcgca aagcgatgaa agagtttgca caggttttgc aaaacaatat
     3061 tcaaaaagcg actgacgata aaaccggtga gaaaccacgc attgagcgtg gcgagacttc
     3121 gccccatact tataaaaaaa cctctcagga ttctgccaaa gcctataaac gagaagctcg
     3181 taagccttat cgtccttggc atcaacataa tcaaaaagga aaataagatg actattttat
     3241 taagctgcct ttttttaggg gtgtttgtcg gctttttagc cggcttgttc ggcattggtg
     3301 gtgggttaat catcgtgccg agtttggtct tcctactgcc catggcaggt gtaccgccgg
     3361 aatatataat gtccaccgca ctcggcacat cattttcaac cattattatt accgcctttg
     3421 catcggcaca acgccatcat aagttaggca atgtcgatat ccaaaccagt aaatatttta
     3481 ttccagcgtt gatggtttcg gtttttctat cgggcttggt ggtaagccgc ctcgatccgc
     3541 aactgatgtc tagaattttt gcggtaatgg tactttacct tgcaggtaaa atgatttttt
     3601 caataaaaag agcaccgaaa aataaaccac ttaccgttca aacgacgata atcggaggtt
     3661 caatcatcgg tgcgttagcc agtatggcag ggatcgcagg tggagccttt attgtaccat
     3721 ttttaaacga tcgaggctta gaaatgaaac gtgccatcgg cacttcctca ttttgcggag
     3781 catttttagg tttatctggc acaatcagct ttattgtaag tggctggggc ttagaaggaa
     3841 tgcctgatta ttctctcggt tatgtttatt taccggctct gtttggtatt acggcaactt
     3901 catttttcac ctcaaaaatg ggggcaagtg ccgccaacgc tctacctgtg ccgatgctga
     3961 aaaaagcctt tgcggtgctt ttggtctgta tcgccatcaa tatgttttta aaataaggac
     4021 actatgaacg aacaatttat ccaatttcca caaattagcc cgattgtgtt tgaaatcggt
     4081 ccgattgccc tgcgttggta tgggctaatg tatttagccg gctttgcctt tgcctactgg
     4141 ctgggaatga gacgggcgaa aaattccaat ggagtttgga caaccgagca agtggatagc
     4201 ttagtctata cttgcttctg gggcgtagta ttaggtgggc gtatcggcga tgtgttcttt
     4261 tatagcttcg accgtttctt acaagatccg ctctacctct ttcgcatttg ggaaggcgga
     4321 atgtcctttc acggcggttt aatcggcgtc attgtagcaa tgatttgggt ttcctaccgc
     4381 caaaaacgca atttctggca gacagcagat tttatcgcac cgctagtacc gttcgggcta
     4441 ggtatggggc gtatcggcaa tttcattaac gatgaacttt gggggcgtgt aaccgatgtg
     4501 ccttgggcgg tgttatttcc aagcggtggc tatcttcccc gccacccgtc acagctttat
     4561 gaagcctttt tagagggtgt ggtgttactc tttatcctca actggtttat tcaaaagcct
     4621 cgcccggcag gtgcggtttc agggttattt ttaatcggct acggcacatt ccgctttatt
     4681 gtggaatact tccgagaaat cgaccataac gtcaatacca ccgcagattt aatcactcgt
     4741 gggcaactac tctctttacc gatgattatc ggcagcatac tgattatggc gtgggcgtat
     4801 aaagtaaaca aagtgaaata aacgtagggg caagtcaata tttgccctca ttacaaaaat
     4861 tgcaaaaaat ttgcagaaaa cgaccgcttg tgtttgaagg atatactatg agtaaaaaat
     4921 acaaagtcat cggtttcgac ttagacggaa cgctaatcaa caccctcccc gatctcactc
     4981 ttgtcgccaa ttcaatgttt ctagaagccg gtttgccaac ggttacacaa gaaaaagtgc
     5041 tgacttggat cggtaaaggg gcagatattt tcttccaaaa tgcggttgct cacaccggca
     5101 gaattttcca agccgatgag ttggtgaaaa tgcgagtgag ctttgataaa tactacgcca
     5161 cctatgtgtg cgaggaaagc acccttttcc caaatgtgaa agaaacctta gaaatcctca
     5221 aagcaaacga ctacccgctg gtggtgatta ccaacaaacc gaccaaatta gtcgagccgg
     5281 tgctttccgc tttcggcatt tatcatttat tcgatgaaac ccttggtggg caatcgctcc
     5341 ccaaaatcaa accgcaccct gacccgatgt tttttatctg cgaaaaattc ggcattaccc
     5401 catcagaatt actttttgtc ggcgactcag aaaatgacgt gattgccgcc aaagccgccg
     5461 gttgtgatgt ggttggacta acttacggct ataactacaa tgtgccgatt gaacaatcta
     5521 acccgacttt tgtgacatct gaatttaaag atgtgttgaa aattgtgggg ttataaagaa
     5581 aacatttgtt ttcttttgat gtttgcttta gactttccga acctaaagat ttaaaggagt
     5641 aaagtatgcc aacaatcgca ttacgtcagg tcggtggctc ggttgcaatg gtgattccgc
     5701 ctgctttttt agaggcactc aagctctccg tcggtagcaa agtggatttg tctttaaacg
     5761 acaaccaact cgtgattaat cctgcccgcc caaaagtgaa atacagctta gaggaattat
     5821 taagacaatc cgattttgag gcagcaagga atgaagaaag ccaatcgtgg ttatccgagc
     5881 cggcaatagg aaaagaaatt ttatgaaacg tggcgatatt tactggctcg atcttgatcc
     5941 aaccttagga cacgaacaat caggcttccg ccccgttgta attgtttccg caactgcatt
     6001 taatctagcc agtaaactgc ctgtggttgt gccgattact aacggtggag cttttgctga
     6061 aagactgggc tttgccgttt cattaaaaga tgccggctta aaaacaacag ggattattcg
     6121 atgcgatcaa cctcgcacag tcgatattgc acaacgccat ggaaaattta tcgaagcctt
     6181 acccgaaagc ctacttgatg aaattatggc aagggtttca gtgatttttg agtaagatac
     6241 aagcggtctg atttgctcta ttttttgcaa aatcatcaat aaatctgacc gcttgtactt
     6301 ttatcccttc ccaataaccg ttaagataac ctatattttt caaatcaaac gagaagataa
     6361 aatgagcaaa cctattgtat ttagtggcgt gcagccctca ggtgaattga ctatcggtaa
     6421 ctacttaggg gcgttacgca actgggtgaa aatgcaagat gattatgact gtgtatattg
     6481 cattgtcgat caacacgcca ttaccgttcg tcaagatccg gagtcgctac gcaaatcaac
     6541 tttagatgtg ttagccctgt atttagcttg tggcattgat ccaaacaaaa gcactatttt
     6601 tattcaatcc cacgtgccgg aacacgccca attagcttgg gtgctgaact gttacaccta
     6661 tttcggtgaa atggggcgta tgacgcaatt taaagataaa tcggcacgct atgaagagaa
     6721 tgtcaatgtg ggcttattta cttatccggt attaatggcg gcagatattc tgctttacca
     6781 agccaaccaa gtgccggtag gcgatgacca aaaacaacat ttggaaatca cccgtgacat
     6841 tgccaaccgt ttcaatgcct tatacggcaa aaaagattcc gaaggcaacc tgatcgagcc
     6901 tgtttttgca gtgccggaag tctttattgc gaaatccggg gcaagagtga tgtcgctact
     6961 tgagccgacc aaaaaaatgt caaaatcgga cgataatcgt aacaatgtga ttggactatt
     7021 ggaagatccg aaagcggtag cgaagaaaat caaacgggca atgaccgatg gcgatgaacc
     7081 gccagtagtg cgttatgacg tacagaataa agccggtgtc tcgaatttat tggatattct
     7141 ctccgccatt accggcaaaa ccattgccga attagaaacc gaatttgaag gcaaaatgta
     7201 tggtcatttg aaaacagaag tagcagataa ggtttccgaa ctattaacca atctgcaaga
     7261 acgttatcac cactaccgcc aagatgaagc attattggaa aaaatctacc gtgaaggggc
     7321 agaaaaagct cgagctcaag ccaaagcaac gttagataag gtatatgaat tagtcggttt
     7381 tgtgcgttac taacacttaa tgaaaaatgc tcttgaggat tcaagagcat ttttgcaaaa
     7441 aagaaccgat tgtcaattat ttacgggcgt tataaatctt cattgcttcc ggcacaaggc
     7501 gggctaagtt ttcttgacgg tcagcatttg atgggtgagt tgagaaaatg gacaagcctg
     7561 aatcgccatt ggctttactc atttttaccc aaacattcgg cgctgctgat gggttgtagc
     7621 ctgattctgc cattaaaatt aacccaactt cgtccgcctc agtctcttgg ctgcgagaga
     7681 atggcttggt ggcaatcaaa tccacgccag tgctgagtaa accttcggtg cttacgcctg
     7741 ttgaggcagt cacagccaca tctgcaatag cacctacaat actggtaata gcgctaaccg
     7801 tacggtctga tttaccgtgt tctttcagtg cgtgagccat ctcgtgtccc attaccactg
     7861 caatttcatc gttcgataaa ttaagtcttt ccactaaccc cgtgtaaaac gccattttac
     7921 cgcccggcat cgcccacgca ttgagttcat ccgatttcaa tacggtaatt tcccaatgaa
     7981 aaggtacacc agtctggttg gctctttccg cataaggctt cattttatga aaaacagtgt
     8041 gaatccgctt agcggtggca gacgaattgt caatcgcatt ttgctgacga gcttgggttt
     8101 tcacttgagc ataactttga gccgcttctt ggttaatgct ttctgttgtg gcacaagcgg
     8161 taatcactgt tgctaaaaaa actgaataac ttaatttttt gatgaatttc attttatatc
     8221 ccacaaaaaa tgccaaacaa atttgcttgg cattcaaaaa taaataatta tttcttagca
     8281 cgctcgaatg attctaagat ttcttggcga gcttcgttta catcgcccca accttctact
     8341 ttcacccatt tgcccgcttc taatgcttta tagctttcga agaagtgctg gatttgtgct
     8401 tttaataacg ccggtaagtc acctacatct ttgatgtggt catactcttt acttaatttg
     8461 gtgtgtggaa cagcaaccac ttttgcatca ccgccggctt catcggtcat ttttaacaca
     8521 ccgacaggac ggcaacgaat caccgaaccc ggttgtaacg gatatggtgt tggcactaat
     8581 acatcaaccg ggtcaccgtc tgatgataag gtgttattca cataaccata gtttgccgga
     8641 tagaacattg cggttgccat aaagcgatcc acaaataatg ccccggtttc tttgtccact
     8701 tcatatttga ttggatcaga gtttgccgga atttcaatta caacataaat atcgtccggt
     8761 aattctttac cggcaggaac taattctaag cccattttgg tttccttcta taataggtga
     8821 aataaacggc gtgattatag cctattttca gaaaaagata aaagcccgtt tctcttttca
     8881 ttttccgcca acaaatccgg ctaatttttt gctaaattgc ttacaaaccg cacactcaac
     8941 catttttcga taaaaaaaac ttgccaaagt tttaaaaatc cgtaagatat gccctcgtta
     9001 cgcaagatta tgggtcatta gctcag
//
LOCUS       DANKBV010000090         8925 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.090, whole genome shotgun sequence.
ACCESSION   DANKBV010000090 DANKBV010000000
VERSION     DANKBV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8925
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.090"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            33..410
                     /locus_tag="PWY43_001735"
     CDS             33..410
                     /locus_tag="PWY43_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136599.1"
                     /translation="MAAEIELSEARTTLDELGFTSPNSLLELYVQQTNTLDQALNKSG
                     YSEETQKLIKLYLVAILAISSGARRIKSEGAPSGASRTFEYDPNALDNLKSAIRSIDP
                     KGCAESLLPKDKVVGFFDVVGGC"
     gene            410..754
                     /locus_tag="PWY43_001736"
     CDS             410..754
                     /locus_tag="PWY43_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136600.1"
                     /translation="MSNLSNWSYTAKATLWQAKGKNDDGVLVFSTPQIISCDYGADRK
                     RARFEIGREQAVKNVIWTEFAHAKLGDYVLIGESEQADPILAGAEEIIHIQRFADTFD
                     RNRDDFALITGG"
     gene            756..1124
                     /locus_tag="PWY43_001737"
     CDS             756..1124
                     /locus_tag="PWY43_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2136601.1"
                     /translation="MGIKVKGIRQVKQRLNQVVGEVQSQKATRAMFRILNAVAPLAAH
                     YTPVDTSTLINSQFTEMEVNGTRLIGRVGYSANYAAYVHDPNIKQNFRKPSAKKEFLT
                     SALNESKATIKSIVKEELSL"
     gene            1121..1501
                     /locus_tag="PWY43_001738"
     CDS             1121..1501
                     /locus_tag="PWY43_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136602.1"
                     /translation="MISYVKAFKQWLEAHSLADGYVVQLYQWEDTQKAKPVIVIQPNS
                     GSPQVSDLSSEHYLLISLVAGKNSGYTIEERARAIMSKILVEPFASFGYIESMGGLPA
                     PIFTEDNRMIFRLPLRIISTNQEE"
     gene            1505..1987
                     /locus_tag="PWY43_001739"
     CDS             1505..1987
                     /locus_tag="PWY43_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2136603.1"
                     /translation="MGSPSPQNNAENLVVGRAVVLEYGTTDAKPQESEWKVAGAMTTK
                     SWDFSPNSVTSEADDAGGFPETLITNSDFSISGDGEWRKRPKSKELGIKDLVVLYANA
                     VKNRTQPYIWVRLKYAGLTILGKMVITALSSEAPTNDLVKFSIEFKVGDSSTIEIAAA
                     "
     gene            complement(2027..2443)
                     /locus_tag="PWY43_001740"
     CDS             complement(2027..2443)
                     /locus_tag="PWY43_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL2136604.1"
                     /translation="MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLL
                     SCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKVLLHNTMQEQAVSKAEIARLTNI
                     RPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG"
     gene            complement(2499..2675)
                     /locus_tag="PWY43_001741"
     CDS             complement(2499..2675)
                     /locus_tag="PWY43_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL2136605.1"
                     /translation="MKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELK
                     TGLVEGVKKQLGLK"
     gene            2772..3443
                     /locus_tag="PWY43_001742"
     CDS             2772..3443
                     /locus_tag="PWY43_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136606.1"
                     /translation="MKMVSYPITEIGEMLIATDGRDFLFRPSFKNIYKIGSPQEIVKI
                     YALLHGCEIREILPHILTKSESHQSYFWSVLNSPVFGRKILQNAMIVMSCCCDDDISL
                     LIGEWLPSKSGVRYKLGKMPPETIINFAKNLMGHGVIGCCELPKKEQSAKNEFSESFD
                     AVEYISLARTHFNMSREDAENLTMTELQQLLKSQMPAENNSYGQFTEEEYDRIMAEYE
                     KRRAA"
     gene            complement(3458..3691)
                     /locus_tag="PWY43_001743"
     CDS             complement(3458..3691)
                     /locus_tag="PWY43_001743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136607.1"
                     /translation="MKKDCTKQDFANWLNENQEVCWIKEVSIERILSCVDDYVAICNA
                     RSKDEFELLNDIRELFYSAHYKDFTPVLSGRFF"
     gene            3860..4318
                     /locus_tag="PWY43_001744"
     CDS             3860..4318
                     /locus_tag="PWY43_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136608.1"
                     /translation="MKKMIVFVASVLSSFVIASDWEIIEAAQNKVKAELIRKSEKKAE
                     LCLNVAQAVLITNQKVQKDTFSMCGEFSTEIVFSDSKAYSLAVCGKVKGKDIIGNPIE
                     SDYIYQRATKDFYFNYNGKKSAYYDPQSDLARLQEVSDSMFNAMQNRYCK"
     gene            4386..4781
                     /locus_tag="PWY43_001745"
     CDS             4386..4781
                     /locus_tag="PWY43_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136609.1"
                     /translation="MDVKVINVLLIILFSAIVIGLFLFQESRLRDPNNHSVIYLEQIQ
                     EMCSLDKIGSYQIDFVRQSGNEYKESILVNDFELAIKTVLSTLRRAKIDSVSVISNTP
                     NLFIIHRAFYNARGSQEGKKLGSIRIAKI"
     gene            4868..7363
                     /locus_tag="PWY43_001746"
     CDS             4868..7363
                     /locus_tag="PWY43_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HDL2136610.1"
                     /translation="MSQAVFYLNNFKELFMTVEVGGVVITADLEFENLLRDTQKVDKA
                     MDDIGKSTLKAEKGLKGLETQATKTAIAVNQATNKMKNIRGVAGQLGYQIQDISIMLQ
                     MGSNASTVIAQQGSQILSIFGPMGAVAGAILAISGAIGGALLPSLFDSTDATELLAAA
                     QKNLSEVVTQTESGVSALSAKIIKLAKVSEDAAKAKIAVAMSDAKIAIKAAGDSSQKA
                     FEQFDGFFGSFTNSSQSIRNAIDELKRYESKGKDVGAVIKELGGTYGGAIAEINALDS
                     VTSEMSKTLGITQSEALGLIKLLAQLEQDKSPDNIKLLAIALSDLSDKNGWANEELNK
                     LTRTINDNSVSALDAETSVRLLESAIKDLQNAIQNSQGALDGNVAKMNQLADAAERKA
                     ATIGKTERETAKYAATLLATTDEAKKNLDSTLSRIDASYDQIEAYQAEQKALQDKKTA
                     DAKAAREAEAAAKKSAQAKKQVVEQLRQMATQYEIAVLRQKGMHLEAVKLEAGMRLGA
                     AATKEQREEAEKLAQSIYGATAASQNFNAIQAQASPVTALDQRHQQQLAQIEEYKILY
                     PQKIMEAEAARAAIEEQYRQQRMEAQWEEWKQASDGARMFGDAVDAVGSSATSTITGL
                     LNGTMSVRDAFASIANTILNSVVQSLVEMGMAQVKQMIMGQTAAKASMAAQMVQAKAL
                     AASFAPAASMVSLATQGANAIPAQAAITSTMSMAQVAAVTGRKLGGPVSARQMYRVGE
                     NNQPEIFKASNGMQYMIPGQSGRVFSNKQSQGQGGGESKTVAVIVNQTNHFSSDEASA
                     NQAAVAKALSNQIKASVRLELQSQMRAGGVLAR"
     gene            7367..7696
                     /locus_tag="PWY43_001747"
     CDS             7367..7696
                     /locus_tag="PWY43_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2136611.1"
                     /translation="MQTFNFKVEQDYTVQNEPSVVILKFADGYEQTAPKGLNHNLKKY
                     QGITVKANKAEAVKIQQFLNAHGGYKKFNWRDRTSDETVTVRCRSWSSTKLGAVIEFT
                     LNFDEVI"
     gene            7718..8164
                     /locus_tag="PWY43_001748"
     CDS             7718..8164
                     /locus_tag="PWY43_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL2136612.1"
                     /translation="MASELIKLEQDALIELWEIDLTHIYSSSNPNQRGEIFRFHNGVS
                     QTQQNIWWQGNEYQAYPIAADGFEISGQGPSNRPTLTVSNLYGVVTVLVEDFGQPVEA
                     KVSSEETVDGNIQMTVELLKKMDQIADQRYRRNQLNDLRTGGTLSK"
     gene            8172..8888
                     /locus_tag="PWY43_001749"
     CDS             8172..8888
                     /locus_tag="PWY43_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL2136613.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRKLQDKDGNAGGVYR
                     FYSGLNELKTSVVWQGRTYDPYPIEATGFERNSSGPSNRPTLTLSNLFGLVTGIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNAKIPSRIIMADTCTWIYRSAECGYTGKQYFDEKDKATSDPKADKCSHCLNG
                     CELRGNQRNFGGFVSVNKLG"
BASE COUNT         2835 a         1716 c         2009 g         2365 t
ORIGIN      
        1 cagaaaggca aaaaagataa aggcaaataa ctatggcagc agaaatagag ctatccgaag
       61 cgcgaacaac gcttgacgaa ctgggtttca cttcgccgaa ctctctgctt gagctttacg
      121 ttcagcaaac gaacacctta gaccaggcgt tgaacaaatc ggggtacagc gaagaaacac
      181 agaaactgat caagctctat ctggttgcta ttcttgcaat ttcatctggg gcgcgccgta
      241 taaagtcaga aggtgcgcct agcggtgcaa gccgaacgtt tgagtatgat ccaaacgcgc
      301 tagacaacct taagtcggcg attcgctcaa ttgatccgaa aggttgcgct gaaagtttgc
      361 tacccaaaga taaagttgtc ggctttttcg atgtggtcgg aggctgctaa tgtcaaattt
      421 atcgaattgg tcctacaccg caaaagccac gctttggcag gctaaaggaa agaacgatga
      481 cggcgtttta gttttttcta cgccacaaat catttcctgt gactacggtg cagatagaaa
      541 gcgcgcacgt tttgagattg ggcgtgaaca agcggtcaaa aatgtgatct ggacggagtt
      601 cgcccacgcc aaattaggcg attatgttct gattggcgaa agcgagcaag ccgatccgat
      661 tttggctggc gcagaggaaa ttattcatat acagcgcttt gctgatacgt tcgaccgcaa
      721 tcgtgatgat tttgcgctga taacaggcgg gtaaaatggg gatcaaagta aaaggcataa
      781 gacaggtcaa gcagcggctg aatcaggttg ttggcgaggt tcagagccaa aaagcaactc
      841 gggcaatgtt tcgcattttg aatgcggttg cccctcttgc ggcgcactat acgcctgttg
      901 atacctcaac gttgatcaac agtcaattca ctgaaatgga agtgaacggc acacgcttga
      961 ttggaagagt gggttattct gctaattatg cggcttacgt tcacgatcct aatatcaagc
     1021 aaaacttccg caagccttca gcgaagaaag agtttttgac ttccgcgctg aacgaatcaa
     1081 aggctacgat caaatccatt gttaaagagg agttgtcact atgatttctt atgtcaaagc
     1141 cttcaaacaa tggttagagg ctcactctct tgcagacggc tatgttgtgc agctttacca
     1201 gtgggaggat acgcaaaaag ccaagcctgt tattgttatt cagccaaata gtggttcgcc
     1261 gcaagtttct gacctgagca gcgaacacta tctgctaatt agccttgttg caggtaaaaa
     1321 ttcaggttac acaatcgaag agcgagcaag ggcgattatg tcaaagattt tagttgagcc
     1381 tttcgcttcc tttggttaca tcgaatcaat gggcggatta cccgccccaa ttttcaccga
     1441 agataatcga atgatttttc gcttaccact tcggattatt tcaacaaatc aagaggagta
     1501 gtttatgggt tctccatctc cacaaaataa cgcagaaaat ctcgtggtgg ggcgtgccgt
     1561 agttttagag tacggtacga ctgatgcaaa gccacaagaa agcgaatgga aggttgcggg
     1621 cgcaatgacg acaaagagtt gggattttag cccaaattcg gttacatcag aggctgatga
     1681 tgctggcggc ttccctgaaa ccttaattac aaactcagac tttagtattt ctggtgacgg
     1741 tgagtggcgc aaacgaccaa aatcgaaaga actaggcatc aaagatctag ttgtgttata
     1801 cgcaaatgcg gtaaaaaatc gcacccaacc ttacatttgg gttcgtttga aatacgcagg
     1861 cttaacaatt ctcggaaaaa tggttatcac tgcattaagt agcgaagccc caaccaatga
     1921 cttagtgaaa ttctctatag agttcaaggt tggcgattca agcactatcg aaattgctgc
     1981 agcgtaaaaa atcccaccct ctataacaag agcggtggga ttatcattat cctagggata
     2041 gttgtagggg ctttcctaag ctagccaaaa tacgaccaat agtgtcgatt tttgtaatat
     2101 ggcgaggggc taaaattcgc tgtacttctg gcggacggat attagttaat cgggcaattt
     2161 ctgccttgct taccgcttgt tcttgcattg tattatgcaa aagcactttt gcgtagatgc
     2221 tctctggcat aaacacaggc acttcgtcgg tatttactgg gcgagtgagc ggaaagcgtc
     2281 tatcttcatc gaaataaatt tcaacacaag atagtagcac gtcttctgcc atttccattg
     2341 cttcttcaaa tgtatcgcct tgggtgatag cttctggcaa atcaggaaat gtcactacaa
     2401 agcccccttc ttcagcgggg gtaaataacg ctggataaaa catattttct ccttattcaa
     2461 gggtgtagtg aaagcccctt aacatcaggg gctttatatt attttaagcc tagttgcttt
     2521 ttaacccctt ccactaagcc tgtttttaac tcttggcttg ggtgtttggg gaggtggctt
     2581 cttttgccct tgtagtagag tttcaaatgc tttgatccat tttcaacctc taccccatta
     2641 gcctttagcc accttaggaa ttcgctttgc ttcactacct cctcctattt gtttaacttg
     2701 ggttcattat atgcaaaaat gctaactttg tcaacagaaa gttagcaaaa atgctaattt
     2761 tttttgttga ggtgaaaatg gttagctatc caattactga aattggtgaa atgcttattg
     2821 caaccgatgg gcgagacttt ttattcagac cgagtttcaa aaatatctac aaaattggaa
     2881 gcccacaaga aattgtaaaa atctatgcac tattgcacgg ctgtgagatt cgagagatat
     2941 tgccgcacat tttaaccaaa tcggagtctc atcaatctta cttttggtca gtgctgaatt
     3001 cgcctgtttt tggcagaaaa attctgcaaa acgcaatgat cgtgatgtcg tgctgttgcg
     3061 atgatgatat ttccctgttg attggcgagt ggctgccgtc aaagtctggc gttaggtaca
     3121 agttgggtaa aatgccacct gagacaatta tcaattttgc aaaaaattta atgggtcacg
     3181 gagtgattgg ttgctgcgaa ttgccaaaga aagaacaatc tgcaaaaaat gaattttcag
     3241 aaagttttga tgctgttgag tacataagtt tagcccgaac tcatttcaat atgagccgcg
     3301 aagacgctga aaatctcacg atgaccgaat tgcagcaact tctaaaaagt caaatgcctg
     3361 ccgaaaataa tagctatgga cagttcaccg aagaagagta cgacagaatt atggcggaat
     3421 atgagaaaag aagagcggct taaatagccg ctaacattta aaagaaccta cctgatagaa
     3481 cgggagtgaa atccttgtaa tgtgctgaat aaaaaagctc tcttatgtca ttgagtagct
     3541 caaattcatc tttggatctt gcgttgcaga ttgccacata gtcatctacg caggataaaa
     3601 tacgttcaat actgacttct tttatccagc atacttcttg gttttcattc agccagttgg
     3661 caaagtcttg tttagtgcag tcttttttca taagttctcc tttttgatta tttgcaaaaa
     3721 aaatcatatc aaatcatccg ttgttatcta ttggcgaaaa taatttttgc gacacagatc
     3781 gcaaaaatag gttttgacgg tggaaaaacg aatcttaatt cgtagaatgg taaaatttct
     3841 ttatttttaa ggtattttta tgaaaaaaat gattgttttt gtagcttctg ttttgtcatc
     3901 ttttgttatt gcatcggatt gggaaataat agaagcggct caaaataagg tgaaagcaga
     3961 gcttataaga aaatcagaaa aaaaagcaga gttatgttta aatgttgctc aggctgtctt
     4021 aattactaat caaaaagtgc aaaaagatac tttctcaatg tgtggtgaat tttcgacaga
     4081 gattgtcttt tctgactcta aagcatatag ccttgcagta tgtggaaagg ttaaaggtaa
     4141 agatattatc ggaaatccga tagagagcga ctatatatac caaagagcaa ctaaggattt
     4201 ttactttaat tataatggca aaaaatcagc ttattatgat cctcaatctg atcttgcaag
     4261 gcttcaagag gtctcagact ctatgtttaa tgctatgcaa aatagatatt gtaagtgatg
     4321 cttgcattgg tttttaaata tttaactcaa ataatttatg tgtttttagt ttaatagggc
     4381 gatttatgga tgtaaaggta attaacgtct tactaattat tcttttttca gcaattgtaa
     4441 taggtttatt cctttttcaa gaatcaagat taagagatcc aaacaaccat tcagtgatat
     4501 atttagagca gattcaagaa atgtgcagct tagataaaat aggctcatac cagattgatt
     4561 ttgtacgaca aagtgggaat gagtacaaag agagtattct tgttaatgat tttgagttag
     4621 caataaaaac cgtattatcc acgttaagaa gggcaaaaat agatagtgtg tcagtaatat
     4681 ctaacacacc taatttattt attatccacc gagctttcta caacgcaaga ggtagtcaag
     4741 aaggaaagaa attaggatcg ataaggattg caaaaattta atgtaacaaa aatatagatt
     4801 aaattgtatt taaccaatct attgctagat aaattgtaaa aaaatcacag aaataaccgc
     4861 ttgtgctatg tcacaagcgg ttttttattt aaataatttt aaggaattgt ttatgacggt
     4921 cgaagttggc ggagttgtaa ttaccgcaga tctagaattt gaaaatctgc taagagatac
     4981 tcaaaaagtt gataaagcga tggatgacat cggaaaatca acattaaaag cagaaaaagg
     5041 gctgaaaggg ctagaaacgc aggcaaccaa aacggcgata gcagtaaatc aagccacaaa
     5101 caaaatgaaa aatatccgtg gagtagcagg tcaacttggt tatcaaattc aagacatctc
     5161 aataatgctt caaatgggtt ctaacgcttc aaccgttatt gctcagcaag ggtcgcaaat
     5221 cttaagtatt ttcggtccaa tgggagctgt tgcaggggcg atcttagcta ttagtggtgc
     5281 tattggtggg gcgttactcc ctagtttgtt tgattcaact gatgcaactg agcttttggc
     5341 agcagctcaa aaaaatctca gcgaagtagt tactcaaacg gaaagtggtg tttccgccct
     5401 ttctgcgaaa atcattaaat tagcaaaagt aagcgaagat gcagcaaaag caaaaattgc
     5461 agtagctatg agtgatgcaa aaatagctat taaggcagca ggggattcat cacaaaaggc
     5521 atttgagcaa tttgacggct tttttggatc gtttacaaat tcaagtcaaa gtattcggaa
     5581 cgcaattgac gaattaaaac gctatgaatc aaaaggtaaa gatgttggtg ccgtaataaa
     5641 agaacttgga ggcacatatg gtggtgcaat tgccgaaatt aatgcgctgg attctgtgac
     5701 aagcgaaatg tctaaaacgc ttggtattac tcaatctgag gctcttggtt tgataaaact
     5761 gttggctcaa ttagagcaag acaaaagccc tgataatatt aaattactcg cgatagcttt
     5821 atctgactta agcgataaaa atgggtgggc gaacgaagag ctaaataagc taaccagaac
     5881 gataaacgat aattcagtat ctgctttaga tgccgaaaca tcagtaagac tcttagagtc
     5941 tgctataaaa gacttgcaaa atgcaatcca aaactcccaa ggtgcattgg atggcaatgt
     6001 agcaaaaatg aatcaacttg cagatgcggc agaaagaaaa gctgcaacga taggcaagac
     6061 tgagcgtgaa actgctaaat atgcagctac gctattagct acaaccgatg aagctaaaaa
     6121 aaatcttgat tccacattaa gtaggattga cgcatcatac gatcaaatcg aagcctatca
     6181 agccgaacaa aaagccttac aagataaaaa gaccgcagat gcaaaagctg ccagagaagc
     6241 tgaagcggct gcaaaaaaat ccgctcaagc caagaagcaa gttgtcgaac agttgcgaca
     6301 aatggctaca cagtacgaga ttgctgtttt gaggcaaaag ggaatgcatc tcgaagccgt
     6361 aaaattagaa gctggtatgc gtttaggagc tgcggcaaca aaagagcaac gagaagaagc
     6421 tgaaaaacta gcacaaagta tttatggagc aaccgcagca agtcagaact ttaacgctat
     6481 acaagcccaa gcatctcctg ttactgcatt agaccaacga catcaacaac agctagctca
     6541 gatcgaagaa tataaaatct tgtatccaca aaaaataatg gaagcagaag ctgcgagagc
     6601 tgcaatcgaa gaacaatacc gccagcaacg aatggaagct caatgggagg agtggaagca
     6661 agcgagcgac ggggcgagaa tgtttggtga tgcggttgat gctgttggtt caagtgcaac
     6721 aagcacaatt acggggctac taaatggcac aatgtccgtg cgtgatgctt tcgcatcaat
     6781 tgcaaatacg atcttaaata gtgtagtaca aagccttgtc gaaatgggta tggctcaagt
     6841 taagcaaatg attatggggc aaactgctgc caaggcttct atggcggcgc aaatggtgca
     6901 agctaaagcg ttagctgctt catttgcacc cgcggcctct atggtttctc ttgccacaca
     6961 gggggcgaat gctattcccg ctcaagctgc gatcacgtca actatgagta tggctcaagt
     7021 cgccgcggtg acaggtcgta aacttggcgg ccctgtatca gctagacaga tgtatcgtgt
     7081 cggcgagaac aatcagcccg aaatcttcaa agcgagcaat gggatgcaat atatgatccc
     7141 cggtcaaagc ggtcgtgtat tcagcaacaa gcagtcgcaa ggtcaaggtg gaggcgaaag
     7201 caaaacagta gctgtcatcg taaatcaaac caatcacttt agcagcgatg aagccagtgc
     7261 aaatcaagcg gctgttgcaa aagcattgag caatcaaatc aaggcttccg tgagattgga
     7321 attgcagtca caaatgcgag ctggtggagt tctagcgagg taatcaatgc aaacatttaa
     7381 tttcaaggtt gagcaagact acactgttca aaatgagccc agtgtcgtca tactaaaatt
     7441 tgccgatggt tacgagcaaa ccgctcccaa agggttaaac cacaatctga aaaaatacca
     7501 aggcattacc gtaaaagcga acaaagcaga agcggtgaag attcagcaat ttctcaatgc
     7561 tcacggtggg tataagaaat tcaactggcg cgatagaaca agcgatgaga cggtaacggt
     7621 tcgttgcaga agctggagct ccactaaatt aggtgcagtc attgagttta cacttaattt
     7681 cgatgaggtg atttaatgcc aaaagccccc caaaaaaatg gcatcagagt tgataaaact
     7741 cgaacaagat gccctgattg agctgtggga gatagatctc actcacatct actctagctc
     7801 aaacccaaat cagcgcggag agatattccg ctttcacaat ggcgtgagcc aaacacaaca
     7861 gaacatctgg tggcaaggca atgaatacca agcatacccg attgccgccg atggttttga
     7921 gatttcgggt caaggaccgt caaatcgtcc aacactcact gtctctaacc tgtacggtgt
     7981 tgtcacggta ttagtagaag atttcgggca acccgtagag gcgaaagtca gcagtgaaga
     8041 gactgtagat ggcaatatcc aaatgacggt ggaactgctg aaaaaaatgg atcaaatcgc
     8101 cgatcaacgc taccgtagaa atcagttaaa tgatttaaga acaggtggaa cattatctaa
     8161 ataggtgaag tatgccaatt agtatttcta accaaatgaa actcgacctt gccaaacttg
     8221 agcaaaatgc aatgcttgat ttgtacgagg tagatttgcg taaattgcaa gacaaagacg
     8281 gcaatgccgg tggggtttat cgtttttact cgggtttaaa tgagctgaaa accagcgtag
     8341 tttggcaagg gcggacttac gacccttacc ccatcgaggc aacaggattt gagcgtaaca
     8401 gcagcggccc aagcaatcgc cctactctta cgctatcaaa tctgtttggc ttagttacgg
     8461 gtattgctaa tcagtttgat gagtgtatcg gggcgattgt tcgccgtcat caggtttacg
     8521 cccaatattt agatgccgtg aattttgccg ggggtaatgc gaaagctgat ccaaatcagg
     8581 aaataatcag tcattttgtg attgagcagc ttaccagcct cacccgagaa acggcgacct
     8641 ttacgctggc attaccgatt gaaactgata atgccaaaat ccccagcaga attattatgg
     8701 cggacacctg cacttggatt taccgctcgg cggaatgtgg ctacaccggc aagcagtatt
     8761 ttgatgaaaa agacaaggca acatcagacc caaaagccga taaatgcagc cattgcctga
     8821 acggttgtga attgcgaggc aatcagcgta attttggtgg atttgtatcg gtgaataaat
     8881 tggggtaatg aatggaattg gaacaacaaa taatcgacta cgccc
//
LOCUS       DANKBV010000091         8913 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.091, whole genome shotgun sequence.
ACCESSION   DANKBV010000091 DANKBV010000000
VERSION     DANKBV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.091"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            16..927
                     /locus_tag="PWY43_001750"
     CDS             16..927
                     /locus_tag="PWY43_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL2136614.1"
                     /translation="MSIKNEKIAVLFGGVSQEREVSLNSGAAVTAALKSLGYNVEGID
                     TKEFPIEKLKEKGIQRVFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFR
                     TKLLWNAVGLPTADMVVVQRGQVVDFDQIIAKLGLPVFVKSSCEGSSVGVFKVKTKEE
                     LAPAIHEALKFDSIVLVEEFLAGNEYSVPVLDGQVLPAVQVIPDGEFYDYHAKYVSDN
                     TQYVVPALNDERQAEVAKLVKAAYDVVGCRGWSRIDVMEDGEGKFRLVEVNTNPGMTS
                     HSIFPKSAATVGISFEKLVERVLELSA"
     gene            927..1718
                     /locus_tag="PWY43_001751"
     CDS             927..1718
                     /locus_tag="PWY43_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL2136615.1"
                     /translation="MGIFRKKNSTNVRLNVLNPKTPVSPRKWLVFIKPLIVLLCTLFI
                     FGVYSNWQNILESWDKTPIRAYALTHKTQFTTNADIRETLSKEPALKGYFGQNIKEVK
                     DKLLSIPWVKDVAVRKLYPDRLSITLLEHKPVAIWNDTNFVSEQGTVFSLPKDRIDKN
                     GLPLLYGPDTEGKSVLEAWNKIRADLNARNLDLTSVSVDNRGSWTIRLSNQVELRLGR
                     GEWTSKIDRFVTIFPEIHIPEGNRLAYVDLRYPHGAAVGFSPIKN"
     gene            1741..3012
                     /gene="ftsA"
                     /locus_tag="PWY43_001752"
     CDS             1741..3012
                     /gene="ftsA"
                     /locus_tag="PWY43_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL2136616.1"
                     /translation="MTKAVESKIIVGLDIGTSKVVAVVGEVLPDGVINVMGAGVCPSK
                     GVDRGGVIDLDAAVNSIQRAIEQAESIADCQIMGVTLAISGQHIVGLNEAGSSTLSGG
                     IVTQDNIESAVDMAKAIKLQDGLETLHMIPQEYYVDRLPATKNPIGLSGMRLQVQAHL
                     IACHQAWLKNLKNAVEHAKLKVDQVVFSGLASSYSVLTEDEKELGVCLIDIGGGTMDV
                     LVYTDGALRYSKVIPFAGNNITDYLARVFTTSRPEAESLKVGYGSAISPPTHNSDKKI
                     EVAGLGGRMARIFTRAQVATVTSQCYNDLLKVVEEELTQLRHELFQKGIKQELIAGVV
                     ITGGGSQIEDIVECAKSIFGSQVRVGYPLNITGLTDYVNKPQYATALGLLQYSHYNYT
                     EQGTSTDSNEDIFAPIGRGTKKIIDFFKSKF"
     gene            3045..4292
                     /gene="ftsZ"
                     /locus_tag="PWY43_001753"
     CDS             3045..4292
                     /gene="ftsZ"
                     /locus_tag="PWY43_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL2136617.1"
                     /translation="MFEPIIEQTQDAIIKVVGVGGGGGNAVDRMSRSADDIKGVEFFD
                     VNTDAQVLRKRTTRQTIQIGASTTKGLGAGADPMVGKQAAEEDREAIANALKGANMTF
                     IAVGMGGGTGTGAAPVVAQIAKEQGSLTVGVVTKPFRFEGPRRMRFADQGIKELSQYV
                     DSLIIIPNDKLRGLGKQTTAVDAFAAANDVLSNCVLGITNMITSSGGSTGADINVDFA
                     DVRTVMSGKGHAMIGTGFAEGEVGEGRAEKAMNDAISSPLLENVDISGASGMLINISA
                     GTDFLLEEVYAMMDLIYGFATEDAAIVFGCNYYPEMDGKVSVTLVATGIGQPEEALHM
                     PHKAQPVYAQQGNHVQATQPTQPNHAVQQPNFTQPSSYGQPNQFSGQPQPLQQPVAPK
                     PQSVDTAQIFNPNSIPGFMRNSQ"
     gene            4324..5244
                     /gene="lpxC"
                     /locus_tag="PWY43_001754"
     CDS             4324..5244
                     /gene="lpxC"
                     /locus_tag="PWY43_001754"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDL2136618.1"
                     /translation="MTKQRTLKQAAKVTGIGLHSGKKVTLTLRPAPANTGVIYARTDL
                     EPVVYFPASADSIRDTQLCTCMVNDDGVRISTVEHLNAAMSALGLDNVIVEVDAPEIP
                     IMDGSSSPFIYLLLDAGIEEQDAPKKFIRIKETVRVEEGDKWAELKPYNKGFKLNFTI
                     DFEHPLIPKNVGSYSIDFSAENFIQQLSRARTFTFMKDVEYLQSIGLALGGSLDNAIV
                     LDDYRILNEDGLRFKDELVRHKMLDSIGDLYMCGYNILGEFSAYKSGHGVNNKLLRTV
                     LENKNAWEFVTFEDKAEVPQGYQVQQEIFI"
     gene            5417..7099
                     /gene="recN"
                     /locus_tag="PWY43_001755"
     CDS             5417..7099
                     /gene="recN"
                     /locus_tag="PWY43_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL2136619.1"
                     /translation="MLTQLTINNFAIVRHLILELNEGMSVITGETGAGKSIAIDALGL
                     CLGYRSESGMIRNGSDKADISATFTMQPHSPAYLWLQEHELLDEDNPQECILRRMINI
                     DGRSKAFVNNRSLPVSQLRELGQYLIHLNGQHAPQLLLKNEYQLEIVDHYATLQPLLM
                     KMAKQYGNWKELNKQVKNFQKQCQENEARKQLLEYQVEELDEFAIKANEFEEMEEKYN
                     ILSSSEELTELSDEITRLLSDDEINANSLIYKALRNAENLAEVDSQYQGILDMLNEAL
                     IQIQEASSEVAQLASKIEQDPELLQELDNRIAQTISLARKHHIQPNELWQYHATLKAE
                     LQELLDFEDNEEQLIQNEKQAYQECITLAEEIYQKRLDAAHKLSEQVTKQIKHLAMEN
                     GEFFIHIEHNLEKMSANGADYVEFNLRSNLGQQAQPLAKIASGGELSRISLAVQVLTA
                     NKLSTPTIIFDEVDVGISGSAATTVGKLLRKLGEKCQVLCVTHLPQVASYGNQQFNVQ
                     KYVENNQTETQMTLLTEAERIHALARLLGGSEITETVLANAKEMLNLAKNNE"
     gene            complement(7146..7221)
                     /locus_tag="PWY43_001756"
     tRNA            complement(7146..7221)
                     /locus_tag="PWY43_001756"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7186..7188),aa:Ala,seq:ggc)
     gene            complement(7302..8741)
                     /gene="gltX"
                     /locus_tag="PWY43_001757"
     CDS             complement(7302..8741)
                     /gene="gltX"
                     /locus_tag="PWY43_001757"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL2136620.1"
                     /translation="MKIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKH
                     NDGEFVLRIEDTDLERSTPEATAAILEGMEWLDLAWEHGPYFQTKRFDRYNQVIDQML
                     EAGTAYRCYCSKERLDEVRAEQEANKEKAKYDQHCLHNPQNDPSQPHVVRFKNPTEGS
                     VVFDDAVRGRIEIANSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNT
                     PRQINILKALGAPIPTYAHVSMINGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLV
                     RLGWGHGDQEIFSRDEMIELFDIHSVSKSASAFNTEKLQWLNQHYIRSLAPSYVAKHL
                     EWHMQTQGIDFTSGPKLEEIIPVLGERCKTLKELAESSRYFYQEFKAYDEKAAAKNFK
                     AEAVAPLAKLLEKLTACNDWTVENIHEAMNQTAAELEIGMGKVGMPFRLAVTGSGQSP
                     SMDITAKLVGKERTLARLQKAIEFIQANS"
BASE COUNT         2808 a         1641 c         1904 g         2560 t
ORIGIN      
        1 tttaaaggat ttcaaatgag tattaaaaac gaaaaaattg ccgtcttatt cggtggcgtt
       61 tcgcaagagc gtgaagtttc actgaattct ggtgcagcgg taacagctgc attaaaaagt
      121 ttaggctata acgtagaagg cattgatact aaagaattcc cgattgaaaa attaaaagaa
      181 aaagggattc agcgtgtatt taatatttta cacggcggta ttggtgaaaa tggcgtactg
      241 caaggtgcgt tagagcaaat gggtattcct tacaccggtt gtggcgtaat ggcatcagca
      301 gtgacgctgg ataaattccg gactaaatta ttatggaatg cagtaggttt accaacagcg
      361 gatatggtgg tggttcagcg tggacaagtg gtcgattttg accaaattat tgcaaaatta
      421 ggcttgccgg tatttgtgaa gtcttcgtgt gaaggctcaa gtgtgggtgt attcaaagta
      481 aaaaccaaag aagaattggc accggcaatt cacgaggcat taaagtttga ttccattgtg
      541 ttggtggaag aatttttagc cggtaatgaa tattctgtac cggtattaga cggacaagtt
      601 ctaccggcgg tacaagtaat tccggacggc gaattttatg actaccacgc aaaatatgtg
      661 tcggataaca cccaatatgt agtaccggca ttaaatgacg aacgccaagc agaggtcgcc
      721 aaattagtga aagcggctta cgatgtggtt ggctgtcgag gttggagccg tattgatgtg
      781 atggaagatg gcgaaggaaa attccgcttg gttgaagtaa ataccaaccc gggaatgacc
      841 agtcacagta ttttcccaaa atcggcagca acagttggta tttcctttga aaaattagtt
      901 gaacgcgtat tggagttaag tgcttaatgg gcatcttccg caagaagaac agcacaaatg
      961 tgcgtttgaa tgttcttaat ccaaaaacac ctgtttctcc acgtaaatgg ctagtattca
     1021 ttaagccatt aattgtgtta ttatgtacac tatttatatt cggtgtgtat agtaattggc
     1081 aaaatatact cgaaagttgg gataaaacac cgattcgcgc ttatgcatta acacataaaa
     1141 cacaatttac aacaaatgca gatattcgtg aaactttgtc taaagaacct gctctaaaag
     1201 gatatttcgg acaaaatatt aaagaggtca aagataagct tttatctata ccttgggtta
     1261 aagatgtggc tgttcgtaaa ctttatcccg accgattaag tattactttg ctagaacata
     1321 aacccgttgc aatttggaat gatactaact ttgtctcaga acaaggaacc gtctttagtt
     1381 taccgaaaga cagaatcgat aaaaatggac ttccattact ctatggtcca gatacggaag
     1441 gaaaaagtgt attagaggca tggaataaaa tccgagctga tctaaatgcc cgcaacttgg
     1501 atttaacctc cgtttctgta gataatcgag gttcttggac aattaggctt tctaatcaag
     1561 tagaattaag attaggaaga ggggaatgga cttctaaaat tgatcgtttt gtaacaatct
     1621 ttcctgaaat tcatatacct gaaggtaata gattagctta tgttgattta cgttatccac
     1681 atggcgcggc ggttggattt agtcctataa aaaattaaac tcagtgaaca caaaaacatt
     1741 atgacaaaag cagtagaatc aaaaattatc gttggtcttg acattgggac atcaaaggtt
     1801 gttgccgttg ttggtgaagt attacctgat ggtgtaatta atgttatggg agctggcgtt
     1861 tgcccttcta aaggagtcga tcgaggtggc gttattgatc ttgacgctgc cgttaattct
     1921 attcaaagag ccattgagca ggcggaatct attgccgatt gtcaaattat gggtgtaaca
     1981 ctggcaatct caggtcagca tattgttggt ttaaatgaag caggatcatc aacattatct
     2041 ggaggtattg ttacccaaga taatattgaa agtgctgtgg atatggcaaa ggcaattaaa
     2101 ttacaagatg gcttagaaac attacacatg attccacaag aatattacgt ggatagattg
     2161 cctgcgacta aaaatccaat tggcttatcg ggtatgcgtt tacaagttca agcacactta
     2221 attgcttgcc atcaagcttg gttaaaaaac ttaaaaaatg cggttgaaca tgctaaatta
     2281 aaagtcgatc aagttgtatt ttcaggtctt gcctctagct attctgtttt aactgaagat
     2341 gaaaaagagc tgggtgtttg cttaattgat attggtggcg gtacgatgga tgtactagtt
     2401 tataccgatg gagcattaag atacagtaaa gtcattccat ttgcaggtaa taacattact
     2461 gattatttgg ctcgtgtatt tacgacatct cgccctgaag ctgaaagcct taaagtaggt
     2521 tatggtagcg caatttcgcc tccaacccat aattcagata aaaaaattga ggtggcagga
     2581 ctaggtggac ggatggcaag aatctttacc cgagctcagg tggcaacggt tacctcacaa
     2641 tgttataacg atttgctaaa agttgttgaa gaggaattaa cccaattacg ccatgaactt
     2701 ttccaaaaag gaataaaaca agagttaatt gccggtgtag tcattactgg tggtggctca
     2761 caaattgaag atattgtaga atgtgctaaa tcaatttttg gttcacaagt tcgtgttgga
     2821 tatccactca atattactgg cttaaccgat tatgtgaata aaccacaata tgcaacagca
     2881 ttagggcttt tacaatatag ccactataat tatactgaac aaggtacaag tacagattct
     2941 aatgaagata tctttgctcc tattggtcgt ggaaccaaga aaatcattga cttcttcaaa
     3001 tcaaaatttt aatagtgtta aaataaacac gcagggagat aaaaatgttt gaacctatta
     3061 ttgaacaaac gcaagatgct atcattaagg tagtgggtgt tggtggtggt ggcggtaatg
     3121 ccgtagacag aatgtctcgc tctgccgatg atattaaagg tgttgaattc tttgatgtaa
     3181 atactgatgc tcaagtttta agaaaaagaa ccacacgcca aactatccaa attggagcaa
     3241 gtacaactaa aggtttaggt gcaggtgctg atccaatggt aggaaaacaa gctgccgaag
     3301 aagatcgtga ggctatcgct aatgcattaa aaggtgcaaa tatgaccttt attgctgttg
     3361 gtatgggtgg aggtaccggt accggagcag cccctgttgt tgctcaaatt gccaaagagc
     3421 aaggctcact aactgtaggc gttgtaacaa aacctttccg cttcgaaggg cctcgccgta
     3481 tgcgctttgc agaccaaggc attaaagagc tttctcaata tgttgattct cttattataa
     3541 ttcctaatga taaattacgt ggtttaggta agcaaacaac agcagtagat gcctttgccg
     3601 cagcaaatga tgttttaagt aactgtgtgc ttggtattac caatatgatt accagttcgg
     3661 gcggttctac cggtgcagat attaacgtgg acttcgcgga tgttcgtaca gtaatgtctg
     3721 gtaagggaca cgctatgatt ggtacaggtt ttgctgaagg tgaagttggt gaaggacgag
     3781 cggaaaaagc aatgaatgat gccatttcta gtccattact tgaaaatgtg gatatttcag
     3841 gtgcaagtgg tatgctcatc aacattagtg ctgggacaga tttcttatta gaagaagttt
     3901 atgctatgat ggacttaatt tatggctttg caacagaaga tgccgcaatt gtatttggtt
     3961 gtaactatta ccctgaaatg gatggaaaag tgagtgtcac tcttgtcgca acaggaatag
     4021 gccaacctga ggaagctcta catatgccac ataaggctca gccagtatat gcacagcaag
     4081 gaaatcatgt tcaagctaca cagcctactc agccaaacca tgcggtacaa caaccaaact
     4141 ttactcaacc gtcaagttat ggtcagccaa atcaatttag tggtcaacca caaccactac
     4201 aacaacctgt ggctccaaaa ccacaatcgg ttgatacagc acaaattttt aacccaaatt
     4261 caattccggg ttttatgaga aatagtcaat aacttccact catttcggaa gtaaggacaa
     4321 aatatgacta aacaaagaac attaaaacaa gcagcaaaag taacaggtat tggcttacat
     4381 agcggcaaaa aagtaacctt aacgttacgc cctgccccag ctaatacagg agttatttat
     4441 gcccgtaccg atttagagcc tgtcgtttat ttccctgcaa gcgcagactc tattcgtgat
     4501 acacaattat gcacatgtat ggtaaatgat gatggtgttc gtatttcaac agtagaacat
     4561 ttaaacgcag caatgtcagc attaggctta gataatgtta ttgttgaagt agatgcacct
     4621 gaaattccga ttatggacgg tagttcaagc ccatttatct atcttttatt agatgccgga
     4681 atcgaagaac aagatgcacc gaagaaattt attcgcatta aagaaacagt acgcgttgaa
     4741 gaaggcgata aatgggccga attaaaacca tacaataaag gttttaaatt gaacttcact
     4801 attgactttg agcacccatt aatccccaaa aatgtaggga gctattccat tgatttttct
     4861 gctgagaact ttattcagca actaagtcga gcgagaacct ttacatttat gaaggatgta
     4921 gaatacttgc aatctattgg tcttgcatta ggtggaagcc ttgataatgc tattgtatta
     4981 gatgattacc gaattctaaa tgaagacggt ttacgattta aagatgaact ggttcgccat
     5041 aaaatgctgg attcgatagg tgacttatat atgtgtggtt ataatatcct aggtgaattt
     5101 agtgcatata aatcaggtca tggcgttaat aataaattat taagaactgt attggaaaat
     5161 aaaaacgctt gggagtttgt tacctttgaa gataaagcag aagtcccaca aggttaccaa
     5221 gtacaacaag aaatttttat ttagtctaaa tggcttaaaa gacgaacaga aatgttcgtc
     5281 ttttattttg caaatttttt ctaatattta accgcttgca acccgcctca ttagcgattt
     5341 tttcgccctt atctatttac tgtttaaaaa aacagttgta ctatataccc atacagttaa
     5401 tcttgtgtga tgtattatgc ttactcaact tactattaat aattttgcga ttgttcgcca
     5461 tcttattctt gaattaaatg aaggtatgtc tgttattacc ggtgaaaccg gtgccgggaa
     5521 atcaatcgct attgatgcct tagggctatg cttgggttat cgttctgaaa gtgggatgat
     5581 tagaaacggt tcggacaaag cagatataag tgcaaccttt actatgcagc cacatagccc
     5641 tgcttattta tggctacaag agcatgaact gcttgatgaa gataatcctc aagagtgtat
     5701 cttacgccgt atgattaata ttgatggacg ctcaaaagcc tttgtaaata accgatcatt
     5761 acctgtttca caacttcgag agttggggca atatcttatt catttaaatg ggcaacacgc
     5821 accacaatta ctcctaaaaa acgaatatca acttgaaata gtcgatcatt atgccacgtt
     5881 acagcctcta ttgatgaaga tggcaaagca atatgggaat tggaaagaac tgaataagca
     5941 ggtaaaaaat ttccaaaaac agtgccaaga aaatgaagct cgcaaacagc tattagaata
     6001 tcaagttgag gaattagatg aatttgcaat taaagcaaac gaatttgaag aaatggaaga
     6061 gaaatacaat atcttgtctt cttccgagga gcttactgag ctttcagatg aaatcacacg
     6121 cttattaagt gatgatgaaa tcaatgcaaa cagtctgatt tataaagcgt tacgcaatgc
     6181 agaaaacttg gctgaggttg atagccaata tcagggcatt ttagatatgc ttaatgaggc
     6241 cctgattcag attcaagaag caagctccga agtggctcaa ttagccagta aaattgaaca
     6301 agatccggaa ttactacagg agctggataa tagaattgca caaactatat cgttagcacg
     6361 caaacaccat attcaaccga atgaattatg gcaatatcac gcgacattaa aagcagaact
     6421 acaggaatta ctggattttg aagataatga agaacaactc attcaaaatg aaaaacaagc
     6481 ttaccaagag tgtataacat tagcagaaga aatttaccaa aaacgtctag atgccgcaca
     6541 taagctctct gaacaagtaa caaaacaaat taaacactta gcaatggaaa acggtgagtt
     6601 ctttattcat atagaacata atcttgaaaa aatgtctgca aatggtgctg actatgtcga
     6661 gtttaattta cgcagcaatt taggacaaca agcccaacca ctggcaaaaa ttgcttctgg
     6721 tggtgagcta tctcgcattt ctctggcagt acaagtgctt accgcaaata aactttctac
     6781 gccaactatt atttttgatg aagttgatgt tgggattagt ggctctgctg ccactacagt
     6841 cggaaagcta ctgcgtaagc taggcgaaaa atgccaagta ttatgtgtaa cccatttacc
     6901 tcaagtggca agttacggca accagcagtt taatgtacaa aaatatgtag aaaataatca
     6961 aactgaaacg caaatgacat tactcacaga agcagagcgt attcacgcct tggctcgact
     7021 attaggcgga agcgaaatca ccgaaacggt tttagctaat gcaaaagaaa tgctaaattt
     7081 agcgaaaaat aacgaatagc caaaaagaaa aatcgcatca atgaatattg atgcgattat
     7141 gaatttggtg gaggtaaacg ggatcgaacc gatgacctct tgcatgccat gcaagcgctc
     7201 tcccaactga gctatacccc cttagataca agcggaataa tatgctttcc gcttgaagat
     7261 gtcaatggct ttaatcaagc aaggtgttga ttgattaaaa actagctatt cgcctgaata
     7321 aactcaatcg ctttttgtaa gcgagctaag gttctttctt taccgactaa tttggcggtg
     7381 atatccattg acggcgattg cccacttcct gttaccgcta aacggaacgg cataccgact
     7441 ttgcccatac cgatttccag ctcggcagcg gtttggttca ttgcctcgtg gatattttcc
     7501 accgtccagt cattgcaagc ggtcaatttt tccaataatt ttgcaagtgg agcaacggct
     7561 tcggctttga agtttttagc ggcagctttt tcgtcgtaag ccttaaattc ttgatagaaa
     7621 tagcggctgc tttcggcaag ctcttttaag gttttgcaac gttcgcccag tacagggata
     7681 atttcttcca attttggacc gcttgtgaaa tcaatgcctt gagtttgcat atgccattca
     7741 agatgttttg ccacataact tggggcaaga ctgcggatat agtgttggtt caaccattgt
     7801 aatttttcag tgttaaatgc acttgccgat ttactgactg aatgaatgtc gaacagctcg
     7861 atcatttcgt cacgagagaa aatctcttgg tcgccgtgtc cccagcctaa acgcacaagg
     7921 tagttaatta aggcttccgg tagatagccg tcatcacgat attgcattac gctcaccgca
     7981 ccgtggcgtt tggagagttt ttgcccgtca tcgccgttga tcattgaaac gtgggcgtag
     8041 gttgggattg gtgctcctaa tgctttcaaa atgttgattt ggcgtggtgt attgttgata
     8101 tggtcttcac cccgcacaac gtgagtaatg cccatatccc agtcgtccac caccacacag
     8161 aaattgtagg tcggcgagcc gtcagtacgg cgaataatca agtcatctaa ttcgctattg
     8221 gcgatttcaa ttcgcccacg caccgcatcg tcaaatacta ccgagccttc ggtcgggttt
     8281 ttgaaacgca caacatgtgg ttggctcggg tcgttttgcg gattgtgtaa gcagtgctga
     8341 tcgtatttcg ctttctcttt atttgcttct tgctctgcac gtacttcgtc taaacgttct
     8401 ttcgagcagt agcaacggta tgccgtgccg gcttctaaca tttggtcgat cacttggtta
     8461 tagcgatcga aacgcttggt ttgaaagtat gggccgtgtt cccacgccaa atccagccat
     8521 tccataccct ctaaaatagc ggcggttgcc tcaggggttg agcgttcaag atcggtatct
     8581 tcaatacgta acacaaattc accatcatta tgcttagcat ataaccaaga gtagagcgcg
     8641 gtcctcgccc cacctacgtg caagtagcca gttgggctag gtgcaaaacg ggtacggact
     8701 tttacattcg gatctaaggg gaaaagggtt tcaattttca ttttatattg cctaattgat
     8761 gattcttaag aaaaacttat ctattttact acgcttgagg gtgaatcata gtaaaaaatt
     8821 aaatttgcgg ggtaaaaaat cagcaaacgg ggtaaaaaga acaaaaaggg tttgacgaaa
     8881 cagcaaaaat ctctataatt cacttccaca aca
//
LOCUS       DANKBV010000092         8729 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.092, whole genome shotgun sequence.
ACCESSION   DANKBV010000092 DANKBV010000000
VERSION     DANKBV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.092"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..147)
                     /locus_tag="PWY43_001758"
     CDS             complement(<1..147)
                     /locus_tag="PWY43_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL2136621.1"
                     /translation="MVNLVIVSHSAKLAEGVGEILAQMAQGGCKIALAGGIEDTSNPI
                     GTDAV"
     gene            complement(157..783)
                     /gene="dhaL"
                     /locus_tag="PWY43_001759"
     CDS             complement(157..783)
                     /gene="dhaL"
                     /locus_tag="PWY43_001759"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDL2136622.1"
                     /translation="MTISKQQILKWLENCQQVMTEKRDYLTELDTVIGDGDHGLNMQR
                     GFSKALEKAHTVQDKDIGTLLKTVGMTLLSQVGGASGPLYGSFFIKAAQGATGKEQLS
                     FNELLALLQQGAEAIVQRGHAQVGDKTMCDLWLPLLEDLAQADSTQPIAQILLQAVEK
                     AENFANQTAPLMAKKGRASYLGERSIGHKDPGATSSYYIFKALAEALQ"
     gene            complement(801..1871)
                     /gene="dhaK"
                     /locus_tag="PWY43_001760"
     CDS             complement(801..1871)
                     /gene="dhaK"
                     /locus_tag="PWY43_001760"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL2136623.1"
                     /translation="MKKLINSIETVLDEQLSGFVKAHSDLTLNTEPVYIYRTTPSNQP
                     KVALISGGGSGHEPMHAGFIGKGMLDGACPGAIFTSPTPDQMFECGLAVDRGEGVLLI
                     IKNYTGDVLNFETATELLADSGVKVATVLVDDDVAVKDSLYTAGRRGVANTVLIEKLL
                     GAAADKGYNLSELAKLGHQLNNNGHSIGIALSACTVPAAGKPSFTLAENEMEFGVGIH
                     GEPGIERRPFENLDSTVKQMFETLIAHGDYQRKIRHWNTQTQQWDEIEESKVALKKGD
                     RVIALVNNLGAVPLSELYAVYDALEKECQTFGLSIERSLVGSYCTSLDMQGISITLLK
                     VDEDILKLWDAPVNTPAMRWGE"
     gene            2038..2991
                     /gene="trxB"
                     /locus_tag="PWY43_001761"
     CDS             2038..2991
                     /gene="trxB"
                     /locus_tag="PWY43_001761"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2136624.1"
                     /translation="MTTKHTKLLILGSGPAGYTAAVYAARANLNPVLVTGMQQGGQLT
                     TTDEIENWPGEFEQITGTELMNKMLKHAEKFETEIVFDHIHSVDLSKRPFTLNGDLYT
                     FTCDALIIATGASAKYLGLPSEEHFKGKGVSACATCDGFFYRNKPVAVVGGGNTAVEE
                     ALYLANLASEVHLIHRRDSFRAEKILLGRLQKRIEEGKIILHTDRTVDEVLGDNMGVT
                     GLRLKSTKEELSEEIKVDGFFVAIGHAPNTAMFEGQLELENGYIKVKSGLEGNATATS
                     VEGVFAAGDVMDHNYRQAITSAGTGCMAALDAERFLDAQEA"
     gene            3001..3756
                     /gene="kdsB"
                     /locus_tag="PWY43_001762"
     CDS             3001..3756
                     /gene="kdsB"
                     /locus_tag="PWY43_001762"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDL2136625.1"
                     /translation="MKFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQLAGASR
                     VIIATDHPEIEALAQSFGAEVCMTSEKHNSGTERLAEVIEKMQIADSEIIVNIQGDEP
                     LIPPVIVTQVAQNLDAYQVNMATLGVKLATREELFNPNAVKVVADKNGMALYFSRASI
                     PFARDSFPDCDDSFVAAQGYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALW
                     HGEKIHIEPAKEAPQVGVDTPEDLERVRAILAR"
     gene            3823..4242
                     /locus_tag="PWY43_001763"
     CDS             3823..4242
                     /locus_tag="PWY43_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDL2136626.1"
                     /translation="MLGNILGSLASSVLSGNNQSTATQLIQNLLASQGGIEGLIAKFQ
                     QGGLDDVLRTWISSDEDNAPVSGEQISNVFGQENIQNVAQEAGVDAADASDLLAQFLP
                     KIVDTLTPNGNVEDLKNFDANDLLAQAAKGILGNLFK"
     gene            complement(4297..5283)
                     /locus_tag="PWY43_001764"
     CDS             complement(4297..5283)
                     /locus_tag="PWY43_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2136627.1"
                     /translation="MSQLIRTLKGHIRDEIIKKGGWVNAHAHADRAFTMTPEKITVYQ
                     NANLQQKWDLVDEVKRNSSVEDYYRRFSQAIELMISQGVTAFGTFVDIDQVCEDRAII
                     AAHKAREVYKNDIILKFANQTLKGVIEPNAKKWFDIGSEMVDIIGGLPYRDEVDYGKG
                     LEAMDILMDKAKSLGKMLHVHVDQFNSPKEKETEQLCDKSIEHGMQGRVVAIHGISIG
                     SHPKEYRKMLYQKMRDSQMMVIACPMAWIDSGRKEDLQPFHNALTPADELIPEGITVA
                     IGTDNICDYMVPLCEGDMWQELSLLSAGCRFTNLEEMANIASINGRKVLGLV"
     regulatory      5567..5710
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5754..6707
                     /gene="thiB"
                     /locus_tag="PWY43_001765"
     CDS             5754..6707
                     /gene="thiB"
                     /locus_tag="PWY43_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDL2136628.1"
                     /translation="MKFILSSLALLSASVYANQPTLTVYTYSSFASEYGPAPKLEQNF
                     EKECGCDVKFVPLDDSVTMFNRLRLEGAKTKADVVVGIDNFLQPEAEKSGLFAEFEAK
                     TKLETQQKIFFPYDTGSYAFIYDKEKLKNPPKSLKELVERQDLKVIYQDPRTSSVGRG
                     FLMWVNQVYGDEAPTAWKTLAKHTVTVGKGWSETYGAFLKGESDLVLSYTTSPLYHKW
                     HENTDKYVAAPFEEGHLVQVENAAIVKSSQQKALANQFLEFLHKPESQHIISYHNVMN
                     PVLTDGVDPAFAAMPTYKTSEFTPPTTETIQKWLADWSKSW"
     gene            6957..7403
                     /gene="dksA"
                     /locus_tag="PWY43_001766"
     CDS             6957..7403
                     /gene="dksA"
                     /locus_tag="PWY43_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDL2136629.1"
                     /translation="MTQAAKTSLGLLALAGVEPYELKKDEEYMNEAQREHFTKILKAW
                     HSQIMEEAERTKSQLQEDMTNFADPNDRASQEEEFSLELRNRDRERKLLKKIEQTLYK
                     IQEDDYGFCETCGVEIGIKRLEARPTADLCIDCKTLAEIREKQLGL"
     gene            7648..>8729
                     /gene="pcnB"
                     /locus_tag="PWY43_001767"
     CDS             7648..>8729
                     /gene="pcnB"
                     /locus_tag="PWY43_001767"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDL2136630.1"
                     /translation="MLHKKFAVNASQYRISPKDFPKNAISIVDKLQIAGYEAYIVGGC
                     IRDLLLEKKPKDFDIATNARPEEIQRIFGRQCRLIGRRFRLAHIVYGREIFEVATFRA
                     GHESHRNDKISKVSDEGMLLRDNVYGTLRDDAERRDFTVNALYYDIKRNLIFDFFNGI
                     EDLRAGKLSLIGEPAIRYQEDPVRMLRAVRFMAKLDMFLDKATASPIRDMAALLTNIP
                     AARLFDESLKLLQTGAGLKTYQLLREYELWQFLFPVLPAFFTVKGDSYAERMITKALN
                     STDERIADKLPVNPAFLFAALLWYPLREKMEQLKNEGGFNSHDAMMLAANDVLAESCK
                     AIALHRRHTSVIRDIWALQFQLMKRTG"
BASE COUNT         2511 a         1796 c         1876 g         2546 t
ORIGIN      
        1 taccgcatca gtgccgatag gatttgaggt atcctcaatg ccgccggcaa gggcgatttt
       61 acagcccccc tgtgccattt gagccaaaat ttcccccacg ccttccgcca gtttagcact
      121 atgtgaaaca atcactaaat taaccattta cccctcctac tgtaatgctt cagctaaggc
      181 tttaaaaata tagtagctgg acgttgctcc cggatcttta tgcccgatac tgcgttcgcc
      241 caagtaactg gcacgccctt ttttcgccat taatggagcg gtttgatttg caaaattttc
      301 tgctttttcg accgcttgta ataagatttg agcaattggt tgagtgctat cggcttgagc
      361 taagtcttcg agtaatggca gccataaatc acacatcgtt ttatcgccaa cttgtgcgtg
      421 tccacgttgt acgatagctt ctgctccttg ctgtagaagt gccagtaact cgttaaagct
      481 cagttgctct ttgcctgtag caccttgagc cgctttaata aaaaatgaac catagagagg
      541 gccgctcgct ccccctactt gcgataataa tgtcattcca acggttttaa gcagggtgcc
      601 gatatcttta tcttgcacgg tatgggcttt ttcaagtgct ttactaaatc cacgctgcat
      661 atttaaaccg tgatcgccat cgccaataac ggtatccaat tcggttaaat aatcccgttt
      721 ttcagtcatt acctgttggc aattttcaag ccattttaag atctgttgtt ttgatattgt
      781 cataacttat cctttcaatc ttattcaccc caacgcattg ccggtgtatt taccggagca
      841 tcccatagtt tgagaatatc ctcatccact ttcagtaatg tgatagagat accttgcatg
      901 tccagcgagg tgcaatatga tccaacaaga ctgcgttcaa tgcttaagcc gaaagtttgg
      961 cactcttttt ctaacgcatc ataaacagca taaagttcgg atagtggcac tgctcctaag
     1021 ttattaacta aagcaataac acgatcacct tttttcaatg ccactttgct ttcttcgatt
     1081 tcgtcccatt gttgggtttg tgtattccaa tggcgaattt tacgttgata atcgccgtga
     1141 gcaattaagg tctcaaacat ttgtttcacg gtactatcca gattttcaaa agggcgacgt
     1201 tcaatgcccg gttctccgtg aatacctacc ccaaattcca tctcattttc agctaaagta
     1261 aatgatggct taccggcagc cggcacagta caagcagata aggcaattcc gatagaatgc
     1321 ccgttattgt ttaactgatg ccctaatttg gctaattcgg ataagttata gcctttatcc
     1381 gctgcagctc ctaacagctt ttcaattaat actgtatttg caacaccgcg gcggccggca
     1441 gtatataaac tgtctttgac tgccacatca tcatcaacca acacggttgc gacttttaca
     1501 ccgctatctg caagtagctc ggtggctgtt tcaaaattca gcacatcacc ggtatagttt
     1561 ttaataatta gcaatacacc ctcgccacga tcaaccgcta aaccgcattc aaacatttga
     1621 tccggcgttg gagatgtaaa gattgctccg gggcaagctc cgtctaacat tcctttaccg
     1681 ataaaaccgg cgtgcattgg ctcgtgccca ctcccaccac cggaaataag ggctacttta
     1741 ggttgattac ttggcgttgt gcggtaaata tagacaggtt ctgtgttaag tgttaaatca
     1801 gaatgtgctt tcacaaaacc agaaagttgc tcatctaaga cggtttctat tgaattaatt
     1861 aattttttca tctttttgcc ccttttaaat gaattagact gtcacaattt acaagaaaca
     1921 cagtcggatt tctgttaact ttgtcacaaa attgaaaaaa gttgagcgaa aacgaaaata
     1981 gagttgaact gaaatttagg ctaaaataaa cattaatatt tattcagagg attcattatg
     2041 accactaaac acaccaaact tttaatttta ggctcagggc ctgccggtta taccgctgct
     2101 gtttatgccg cccgtgccaa tctaaacccg gttttagtca ccggtatgca acaaggcgga
     2161 cagctcacta caaccgatga aatcgagaac tggccggggg aatttgaaca aattaccggc
     2221 acagagttaa tgaacaaaat gctcaagcac gctgaaaaat ttgaaactga aatcgtgttt
     2281 gatcatattc attcagtgga tttatctaaa cgcccattca cgctaaacgg agatttatac
     2341 actttcacct gtgatgcgtt aattattgcc accggtgcgt cggcgaaata tttaggtttg
     2401 ccttctgaag agcatttcaa aggcaaaggc gtgtcggctt gtgcaacttg tgatggtttc
     2461 ttctatcgca ataaaccggt tgcggtagtc ggtggtggga atactgctgt ggaagaagca
     2521 ctttatttgg caaatttggc aagtgaagtg catttaattc accgccgtga tagcttccgt
     2581 gctgaaaaaa tcttgcttgg tcgtctgcaa aaacgtattg aagaaggcaa aattattctg
     2641 cacacagacc gtacggttga tgaggtgtta ggcgataata tgggcgttac cggcttacgt
     2701 ttaaaatcaa ccaaagaaga attgagcgaa gaaattaagg ttgatggttt ctttgtggcg
     2761 attgggcacg caccgaatac ggcaatgttt gaaggtcaat tagaacttga aaacggctac
     2821 attaaagtga aatcaggttt ggaaggcaat gctactgcca cttctgtgga aggcgtattt
     2881 gctgccggtg atgtgatgga tcataactat cgccaagcga ttacctctgc cggcacaggt
     2941 tgtatggcag ccctggatgc agaacgtttc ttagatgctc aagaagcata aggatagcct
     3001 atgaaattca ctgtaattat tcctgctcgt tacgcttcca gccgtttgcc tcgtaagccg
     3061 ttgttggata ttgcaggcaa accgatgatt caacacgttt gggaaaaagc tcagcttgcc
     3121 ggtgcaagcc gtgtgattat tgccaccgat caccctgaaa ttgaagccct cgctcaatct
     3181 tttggggcgg aagtctgtat gacttcggaa aaacacaatt ccggcactga gcgtttggcg
     3241 gaagtgatcg aaaaaatgca gattgccgac agcgaaatta ttgtgaatat tcagggtgat
     3301 gaaccgctga ttccaccggt aattgttact caggtagctc aaaatttaga tgcttatcaa
     3361 gtcaatatgg caacactcgg tgtgaaatta gcgacccgag aagagctatt taacccaaat
     3421 gcggtgaaag tggtggcaga caaaaacgga atggcacttt atttttcccg agccagcatt
     3481 ccgtttgcca gagacagttt tccggattgt gatgacagtt ttgttgccgc tcaaggttat
     3541 ttacgccata tcggcattta cgcttaccga gccggttttg tgaaacaata tgtggcgtgg
     3601 cagccgacac aattagaaca gcttgagtcc cttgagcaac taagagcctt atggcacggt
     3661 gaaaaaattc atatcgaacc ggcgaaagaa gccccgcaag tgggggtgga tacgccggaa
     3721 gatttggaaa gagtgcgagc cattttagct cgttaaagtg attacaagcg gtcgaagttt
     3781 gctgtttttt tgcaaaattg taccgcttgc ataggagaaa ttatgttagg aaatatttta
     3841 ggttcattgg cttcttcagt attgagtggc aataaccaat caactgcaac tcaattaatt
     3901 caaaatttac tcgcctcaca aggtgggatt gaagggctga ttgccaaatt ccaacaaggc
     3961 ggattagatg atgtgttacg cacttggatc agcagcgatg aagacaatgc acctgtttcg
     4021 ggcgaacaaa tcagtaatgt gttcgggcaa gaaaacattc aaaatgtggc tcaagaagcg
     4081 ggtgttgatg ctgctgatgc gagcgattta ttagcccagt tcttgccaaa aatcgtggat
     4141 actttaaccc cgaatggtaa tgttgaagac ttaaaaaact ttgatgcaaa tgacttatta
     4201 gctcaagcgg caaaagggat tttgggtaac ttgtttaaat aatcggttat gctgttatta
     4261 aaggcgaacc taagggttcg cctttcgttt attcagttag actaaaccca gtacttttcg
     4321 accattgata ctggcaatat ttgccatttc ttctaggtta gtaaaacgac agcctgctga
     4381 taaaaggctg agttcttgcc acatatcgcc ctcgcaaagc ggtaccatat aatcacagat
     4441 attatctgtg ccgatagcaa cagtaatgcc ttcaggaatc agctcatcag caggggttaa
     4501 agcgttgtgg aacggctgta aatcttcttt acgtccgcta tcaatccacg ccatcggaca
     4561 ggcaatgacc atcatttggc tatcacgcat tttttggtaa agcatttttc gatattcttt
     4621 cgggtgcgag ccgatggaaa tcccgtgaat ggcaaccact cgcccttgca taccatgctc
     4681 aatcgattta tcacacagtt gttcggtctc tttttcttta ggtgagttaa attgatcaac
     4741 gtggacgtgg agcattttgc cgagcgattt ggctttatcc attaaaatat ccattgcctc
     4801 caagcctttg ccgtaatcga cttcatcacg atagggcaag ccgccgataa tatccaccat
     4861 ttccgagccg atatcgaacc actttttcgc attaggctca atcacgcctt tcaacgtttg
     4921 gttagcaaat ttcaagatga tgtcgttttt atatacttca cgggctttgt gagcggcaat
     4981 aatggctctg tcttcacata cttggtcgat atccacaaaa gtaccaaatg ccgttacccc
     5041 ttgtgaaatc atcaattcaa tcgcttggga aaagcggcgg tagtaatctt ctacgcttga
     5101 attacgtttt acctcatcaa ccaaatccca tttttgctgt agattcgcat tttggtaaac
     5161 ggtgattttt tcaggcgtca tcgtaaatgc acgatctgcg tgggcgtgtg catttaccca
     5221 accgcctttt ttgatgattt catcacgaat atgtcctttt agggtacgaa tcaattggct
     5281 catttttatt ccttttctct ctgttaatgt ggctcaaccg cttgcggtaa ttgcaagcgt
     5341 gcgaaaaaaa atcgctataa cagcgatttt gaatggtttg aaatgaatag aaaaacagct
     5401 acgcctctaa agttgaagcg tagctgttgg gaatttgatt taaccgatgt tgtgcaattc
     5461 atttctagtt ccttgtttgg actagggcgg tagtatacgg atttttaata caaatagcaa
     5521 acgtttgctt tggttgaaat ctgcctgcaa actttttaca atgaagccgt tctcattggg
     5581 gtgcaacaag cggttgtttt tgcgtaaaaa tttgcaaatt tttgccgaaa tttaaccgct
     5641 tgtggctgag aaatacccat agaacctgat ctgattaata tcagcgtagg gatttgagat
     5701 agcaacttgc ctttcttaaa tccttctatt catttaattt agaaggaatt gttatgaaat
     5761 ttatcctctc ttcacttgcg ttattaagtg caagtgttta tgccaatcaa ccgaccttaa
     5821 ccgtttacac ttacagctcg tttgcttcgg aatatggacc tgctcctaaa ttagagcaga
     5881 attttgagaa agagtgtggc tgtgatgtca aatttgttcc gcttgacgat agcgtaacga
     5941 tgttcaaccg cttgcgtttg gagggagcaa aaaccaaagc tgatgtggtg gtcggtattg
     6001 ataatttcct gcaacctgaa gccgaaaaaa gcggcttgtt tgccgagttt gaagcaaaga
     6061 ccaaattaga aacccaacag aaaattttct tcccttatga cacgggttct tatgctttta
     6121 tttacgataa agagaagctg aaaaatccac caaaaagttt aaaagagttg gtggaacgcc
     6181 aagatttaaa agtgatttac caagacccac gtaccagcag cgttgggcgg ggcttcttaa
     6241 tgtgggtaaa tcaagtgtat ggtgatgaag ctccaactgc ttggaaaaca ttggcaaaac
     6301 atacggtaac ggtaggcaaa ggttggtctg aaacttatgg agcgttctta aaaggcgagt
     6361 cggatttggt actaagctat actacatcgc ctttatatca taagtggcac gaaaacaccg
     6421 ataaatatgt ggctgctccg tttgaggaag ggcatttggt gcaagtagaa aatgcggcta
     6481 ttgtcaaaag cagccaacaa aaagcattgg caaatcaatt tcttgaattt ttgcacaaac
     6541 cggaatcgca acatattatt tcttaccaca acgtaatgaa tccggtttta accgacggtg
     6601 ttgatccggc ttttgcggca atgccaacct ataaaaccag tgaatttacg ccaccgacta
     6661 ctgaaactat ccaaaaatgg ttagccgatt ggtcaaagag ctggtaaaaa gtgtttgaaa
     6721 aatagccgaa tggatgttcg gctatttttt tgtgcaatca aatcaatttg aatgcaaaat
     6781 ttttgcaaat tcatttgatt aatgagtcgt ttttgtgtta tttatagccg ccattattta
     6841 aaacccgaag atgttgattt tttcgtatag tgcggttaca aggaagttag tctgtattcg
     6901 ggcaataatt ttaacaataa aacttccaat aaacagggaa caaggagtaa atatctatga
     6961 ctcaagcggc taaaacctca ctcggtttat tagcattagc aggcgttgag ccttatgagc
     7021 ttaaaaaaga cgaagaatat atgaatgaag cccaacgtga gcatttcact aaaattctga
     7081 aagcatggca tagccaaatt atggaagaag ctgagcgtac aaaaagccaa cttcaagaag
     7141 atatgactaa ttttgctgat ccaaatgacc gtgcaagcca agaagaagaa tttagcttag
     7201 aactacgtaa tcgagatcgt gagcgtaagt tactgaaaaa gattgagcaa acattataca
     7261 aaattcaaga agatgactac ggtttttgcg aaacttgtgg tgtagaaatt gggattaaac
     7321 gtttagaagc acgcccaaca gctgatttat gtattgactg taaaacttta gctgaaattc
     7381 gagaaaagca gctaggctta taatcaactt tacagggtgc ctctttttta agaggcattt
     7441 actatttaga ggtgtattat ttttaattat attaaatcat tattttccaa gcctaaaaag
     7501 tcggctcaga aaaatgaaaa ggtagagagt gaaaaaagag cggaaagaga acccaaaact
     7561 tcccgttcta aacgcccagc cccgaactta acggaccaaa agacttatag aggtgggaaa
     7621 aaacaacaag ataatcctcc ctcccatttg ttacacaaga aatttgcggt gaatgccagc
     7681 caatatagaa ttagcccgaa ggattttcct aaaaatgcga tttcgattgt cgataaattg
     7741 cagattgccg gttatgaagc ttatattgtg ggtggctgta ttcgagatct actcttggag
     7801 aaaaagccta aagatttcga tattgcgacc aatgcgcgcc ctgaagaaat tcagcgtatt
     7861 ttcggcagac aatgtcgtct gattggtcgc cgtttccgct tggcacatat tgtttacggg
     7921 cgtgaaattt ttgaagtagc gactttccgt gccgggcacg aatctcatcg caatgacaaa
     7981 atttctaaag tcagcgatga aggaatgctc ctacgtgata acgtttacgg cacactccga
     8041 gatgatgccg aacgccgtga tttcaccgta aacgccttat attacgatat aaaacgcaat
     8101 cttatttttg atttcttcaa cggcattgaa gacttacgag cgggtaaact ttcgctaatt
     8161 ggtgagccag ccattcgtta ccaagaagat ccggtgcgta tgttgcgagc cgttcgcttt
     8221 atggcgaagt tggatatgtt tttggataaa gccactgcct ccccaattcg ggatatggct
     8281 gcactgttaa ccaacattcc ggcagcccgt ttatttgatg agtcactcaa attattacaa
     8341 accggtgcag ggctgaaaac ctatcaactt ttgcgtgaat atgaactatg gcaattcttg
     8401 tttccggtat tgccggcatt ttttactgtc aaaggcgatt cctatgccga aagaatgatt
     8461 accaaagcgt taaattcaac tgatgagcga attgccgata aattaccggt caatcctgct
     8521 tttttatttg ctgcactact ttggtatccg ttacgggaga aaatggaaca gcttaaaaat
     8581 gaaggtggct tcaacagcca cgatgcaatg atgttggcgg caaatgatgt gttggcagaa
     8641 agttgtaagg cgattgcgtt acaccgcaga cacacttcgg ttattcgaga tatttgggca
     8701 ttgcaattcc aactgatgaa acgaaccgg
//
LOCUS       DANKBV010000093         8647 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.093, whole genome shotgun sequence.
ACCESSION   DANKBV010000093 DANKBV010000000
VERSION     DANKBV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.093"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..865)
                     /locus_tag="PWY43_001768"
     CDS             complement(<1..865)
                     /locus_tag="PWY43_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2136631.1"
                     /translation="MSSRGKILNTISSLQHSLTKTEKKIAAAILSQPELLSQCSLSEV
                     AKQLDVGEATFIRFCRTLGFKGYTDFKLELAIELATNQDNRVLLDTDVSESDTSKDIA
                     EKLKVSLDNVIEETINLLDFNVLEKVVEELRKAKRIFLFGVGSSGLTAEDAKHKLMRI
                     GLQTDAVTNNHFMYMQAALVKEGDLVIGISHSGYSEEIVKSLRFSRANKATTVAITHN
                     LRSPVTEEANYVLINGNRQGHMQGDSIGTKMSQLFVLDLIYTLLVKAEPENALKQKQK
                     TLNVILEQRVKG"
     gene            complement(875..1339)
                     /locus_tag="PWY43_001769"
     CDS             complement(875..1339)
                     /locus_tag="PWY43_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL2136632.1"
                     /translation="MILADLSRDDYLKSLPPVFARLCKKLKSLDLVNLELGWQELENG
                     IRMNVMSFETTPAEGKKAELHRKFIDIQLLIDGEEMIEYGLSQPDLSKYDEYRDEDDY
                     QLTAEIDDKNNLILKPNMFAIFLPYEPHKPGNSVNGNKTLKKLVVKVPVELI"
     gene            complement(1586..3088)
                     /gene="ppx"
                     /locus_tag="PWY43_001770"
     CDS             complement(1586..3088)
                     /gene="ppx"
                     /locus_tag="PWY43_001770"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDL2136633.1"
                     /translation="MSSNIAPREFAAIDLGSNSFHMIVARIVNGSIQVLSRLKRRVRL
                     ADGLDENLVLSQDAIDRGIACLALFAKRLEGFSAENVKVVGTYTLRRAINNQAFLEQA
                     KAVFPYPISIISGQQEARLIYSGVSHTQPETGRKLVVDIGGGSTEMTIGDDFTPIRAE
                     SRHMGCVSFAKRFFPQGELTIERFDSAYQLAMEKIEDLMWEYKELGWDYALGSSGTIK
                     TVHQVLIANGYRDGLITESRLQKIIDICLEFKSLNEIKLRGLLDERADVLVPGLAILL
                     ALFHTFRIDVLRYSNGALREGVMYGLEKSFQVGDIRQRTAEALAEQFALDKQQAKRVE
                     HTALILFDQIKTWKNRRQVQDLRSMLKWASLLHEIGIVVNHNNVHRHSAYLIANRDLP
                     GFDFEQQTLLSVLMRYHLKGFKRSDIRSINRYQCRDVLTLVRVFRLAVLLNRSRQATV
                     VPKQLNLVIENNAWQLTFEQGFLANNPLISIDLEEEQKILASTNLQFAVS"
     gene            3284..3613
                     /locus_tag="PWY43_001771"
     CDS             3284..3613
                     /locus_tag="PWY43_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDL2136634.1"
                     /translation="MSEVNKSFHDMLEYVHLYRLKNKLHRETADNDRKIRDNQKRVLL
                     LDNLTQYITDEMSIEEVRAIISNMRDDYENRVDDYMIRNAELSKQRREIRQKMASHKQ
                     APTNKKA"
     gene            complement(3677..5107)
                     /locus_tag="PWY43_001772"
     CDS             complement(3677..5107)
                     /locus_tag="PWY43_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDL2136635.1"
                     /translation="MSTLTYLQKIGRALMVPVAALPAAALLMGIGYWIDPDGWGTNSQ
                     LAALLIKSGAAIIDNMSILFAVGVAFGMSKEKDGAAALSGLVGYLVITTLLSPGTVAQ
                     LKGIAVDQVPAAFGKINNQFIGILVGVISAEIYNRFYQVELPKALSFFSGKRLVPIVV
                     SFILLYVWPVIFGALVSFGESIKDMGALGAGIYAFFNRLLIPVGLHHALNSVFWFDVA
                     GINDIPNFLGGAKSLAEGKAVIGVTGMYQAGFFPVMMFGLPAAALAIYHSAKPAKKMQ
                     VASIMFAAALASFFTGITEPLEFAFMFVAPVLYVVHALLTGLSVYIAASMEWIAGFGF
                     SAGLVDLVLSTRNPLAVQWYMLLAQGIVFAVIYYVLFRVVIQAFNLKTPGREEDSEVQ
                     TVNTVSRSEQAAKFVEALGGKENITSIDACITRLRLTLNDTAKADETKLKALGSKGNI
                     KLGENGYQVILGPEAEFVADAMKKLN"
     gene            5670..7022
                     /gene="lysC"
                     /locus_tag="PWY43_001773"
     CDS             5670..7022
                     /gene="lysC"
                     /locus_tag="PWY43_001773"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDL2136636.1"
                     /translation="MSHLSVAKFGGTSVANFDAMTSSANIVIADANTRVVVLSASAGV
                     TNYLVELANGCEKEHRDEVIAAVRQIQYNIIEKLQHQDAVKAEIDELLERIETLAESA
                     SLATSAALSDELISHGEMMSTKIFTQLLIERGHPAVWVDVRDVVATNSHYGKAAPNDE
                     KTQQQSDNVIKPLIASGKIVITQGFIGRDDEGKTTTLGRGGSDYSAALLAEVLNANDV
                     LIWTDVPGIYTTDPRVVPNAQRIDTMAFNEAAEMATFGAKVLHPATLLPAVRSNIPVY
                     VGSSKAPEQGGTWVTRDPQPRPTFRAIALRRNQILLTLSSLSMLHAQGFLANVFAILA
                     KHKISVDVITTSEVSVALTLDKTGSASSGADMLSAELLDELNAYCNVQVESDLALVAI
                     IGNNLHAQSGVAKKLFHTLENYNIRLISYGASSNNVCTLVKNAEADDVVRSLHKGLFE
                     "
     gene            complement(7093..>8647)
                     /gene="parC"
                     /locus_tag="PWY43_001774"
     CDS             complement(7093..>8647)
                     /gene="parC"
                     /locus_tag="PWY43_001774"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2136637.1"
                     /translation="EIARMYEQGRGSIKMRAVWKKEEGEIVISALPHQASPSKIIEQV
                     ATQMRNKKLPMVDDIRDESDHENPIRIVIVPRSNRIDFDALMDHLFATTDLEKSYRVN
                     MNMIGLDGKPAVKNLLTILNEWLSFRRTTVTRRLNYRLDKILNRLYILDGLMIAFLNI
                     DEVIEIIRNEDEPKAELMARFNLTDVQAEAILNLRLRHLAKLEEHQLQAEKSELEKER
                     DELQLILGSERRLNSLIKKEIQADAKAFASPRRSPLVERAESKAIAESDLTPIEDVTV
                     ILSEKGWVRCAKGHDIDVQALSYRAGDDYLAHARGRSNQPVVFLDSTGRAYALDPTSL
                     PSARSQGEPLTGKITLPEGATVQQVLMANSETKVLMASDSGYGFICTFEDLVSRNKAG
                     KAVISLTENAKVLPPQLLENDENLSLVAMSNVGRMLVFPVSELPALSKGKGNKIINIS
                     AAAAKSGDEYLARLLVIKPTNSLVFVSGKRKITLKPSDIDNYRGERARKGSQLVRGLS
                     TNSTVEIVE"
BASE COUNT         2648 a         1911 c         1563 g         2525 t
ORIGIN      
        1 agcctttaac acgttgttcc aaaatcacat tcagtgtttt ttgtttctgt ttcaacgcat
       61 tttcgggttc ggctttaact agtaaggtat aaattaaatc aagaacgaaa agttgtgaca
      121 ttttagtacc gatagaatct ccttgcatat gcccctgtcg attaccatta attaacacat
      181 aattagcctc ttccgtgacg ggggaacgta aattgtgtgt aatcgcaacc gttgtcgctt
      241 tatttgcacg agaaaaacgt agtgatttca caatttcttc agaatagcct gaatgactaa
      301 tccctataac taaatccccc tctttcacca atgccgcctg catatacata aaatgattat
      361 ttgtgactgc atcggtttgt agcccaatac gcattaattt atgttttgca tcttctgcgg
      421 ttaacccaga agaacccact ccaaataaaa agatccgttt tgccttacgt aattcttcaa
      481 caactttttc caacacgtta aagtcaagca aattaatagt ctcttcaata acgttatcta
      541 aactcacttt gagcttctca gcaatatctt tcgatgtatc cgattctgaa acatccgtat
      601 caagcaatac tctattatct tgatttgtag ccaactctat tgcaagctct aatttaaagt
      661 cggtataacc tttaaaacct aatgtacgac aaaaacgaat aaaagttgct tcaccaacat
      721 ctaattgttt ggccacttca gataatgaac attggctaag taattccggc tgagataaaa
      781 tagcagcagc tattttcttc tctgttttgg ttaaactatg ttgtagcgag ctaattgtat
      841 taagaatttt tccccgacta gacatagtag cctcctaaat caattcaaca ggtactttca
      901 ccaccagttt tttcaatgtt ttgttgccat tcaccgaatt tccaggctta tgtggttcat
      961 aagggaggaa aatagcgaac atatttggct tgaggatcaa gttattttta tcatcaatct
     1021 cagctgttaa ttggtagtca tcttcatctc gatattcatc atacttagat aaatctggct
     1081 ggcttaaccc gtattcaatc atttcttcgc cgtctatcaa taattgaata tcgataaatt
     1141 ttcggtgtaa ctctgctttt ttcccctcag ctggcgtagt ttcaaatgac attacattca
     1201 tgcgaatacc attttctaat tcttgccagc ctaattccaa attaactaaa tctaaacttt
     1261 ttaatttttt acaaaggcga gcgaatactg gtggaagtga ttttaaataa tcatctcttg
     1321 ataaatcggc taatatcata taattctcct aaaaataact aattctaata atctgctcca
     1381 tactactaaa atttattaag atacactcca atagaagtag gataattcgt aaaatatata
     1441 atctcaataa gcaaacatca aaaatgagaa ggtgatcaaa ttttaaaaaa tactttttgc
     1501 aaaaaattag ctaaatttga ccgcttgttc gcaaaaggga atgcaataaa cgaaaaagga
     1561 agccttcgct tccttcattt aaatgttaag agaccgcaaa ctgaagattt gtactcgcca
     1621 atattttttg ctcttcttct aaatctattg aaatcagtgg attattcgct aagaagccct
     1681 gttcaaaggt aagttgccaa gcattatttt caatcaccaa attcaactgt ttcggcacta
     1741 cagtcgcttg gcgagaacgg tttaatagca ccgctagccg gaaaacccgt accaacgtta
     1801 atacatctct acattggtaa cggttgatac tacgaatatc cgagcgtttg aatcctttaa
     1861 ggtggtatcg cattaatacc gatagaaggg tttgctgttc aaaatcaaat cccggtaaat
     1921 ctcggtttgc aatcaaataa gcagaatgac gatgcacatt attgtggttt acaacaatac
     1981 caatctcgtg taataaactt gcccatttca gcattgaacg cagatcttgc acttggcggc
     2041 ggttcttcca agttttgatt tggtcaaata aaatcaacgc cgtatgctcc accctttttg
     2101 cttgctgttt atctaaagca aattgctcag ctaacgcttc tgcagtccgt tggcgaatat
     2161 ccccaacttg gaaacttttc tccaaaccat acattacccc ttctcttaaa gccccgttgg
     2221 aataacgtaa tacatcaatt ctaaaagtgt gaaaaagtgc taaaagaata gctaagcccg
     2281 gaactagcac atctgctcgt tcatcaagta agcctcgtag cttaatctca tttaacgatt
     2341 taaactctag gcaaatatca ataattttct gtaggcggct ttctgtaatt aagccatcac
     2401 gatagccatt ggcaatcagc acttgatgga cggttttaat cgtaccggaa gaacctaaag
     2461 catagtccca acctaactct ttgtactccc acattaaatc ttcgattttt tccattgcaa
     2521 gctgataggc actatcaaaa cgctcaatcg ttaattcccc ctgcgggaaa aagcgttttg
     2581 caaaactcac acaacccata tgacgacttt ccgctcgaat cggggtaaag tcatcaccaa
     2641 tagtcatttc cgttgaacca ccgccaatat ctaccactaa ttttctgcca gtttcgggtt
     2701 gggtatggct aacgcccgaa taaatcagcc tcgcctcttg ttgtccggaa ataatggaaa
     2761 tcgggtaagg aaatactgct ttcgcttgct ccagaaaagc ttggttattg attgctctac
     2821 gtaaggtata agtgccaacc accttcacat tctctgcaga gaacccttct aaacgttttg
     2881 caaataaagc taaacaagca atgcctctat caatcgcatc ttggcttaat actaagtttt
     2941 catcaagccc atctgccagc cgtacacgcc gtttcaaacg ggaaagcact tgaatggaac
     3001 catttacgat acgggctaca atcatatgaa agctgttcga gcctaaatca atagctgcaa
     3061 actctctagg agcaatattt gatgacataa tgaaaccctg taccaataac tgaaataagt
     3121 aagatattac catttttgag ccctagatat cactacaaat ttagcgttat tcgcgagaat
     3181 taatttaaat cataaaatgt gataaatttt tttgacgaat tgacggaaaa agaataggat
     3241 ttacgagctt acttaattca acactacatc aagggagatt gatatgtctg aagtgaataa
     3301 atcattccac gatatgctgg aatatgtgca tctttatcgt ctaaaaaaca aactgcaccg
     3361 tgaaacggcg gataatgatc gcaaaattcg agataatcag aagcgtgtac ttttattaga
     3421 taacctaacg caatatatta ccgacgaaat gtctatcgag gaagttcgag cgattatttc
     3481 caatatgcgt gatgactatg aaaatcgagt agatgattat atgatccgta atgcggaact
     3541 ttccaaacaa cgccgtgaaa tccgccaaaa aatggcctct cacaaacaag caccaaccaa
     3601 taaaaaagca taaaaagcca agcggtctga tttgcaaaaa aatatgcaaa tcagaccgct
     3661 tgtgattaag cagagattaa ttcagctttt tcattgcatc tgccacaaat tctgcttccg
     3721 gtcccaaaat cacttggtag ccattctcgc ctaatttgat attacctttt gaccctaacg
     3781 ctttcaattt ggtttcgtct gctttcgccg tatcatttaa ggttaggcgt aagcgggtaa
     3841 tacaagcatc aatgctggta atattctctt taccacctaa tgcttcaaca aatttggcag
     3901 cttgttcgct acgagaaaca gtattcactg tttgtacttc gctatcttcc tcacgccccg
     3961 gcgtttttaa attaaaggct tggatcacta ctcggaataa tacatagtaa attactgcga
     4021 atacgatacc ttgggccaat aacatatacc actgcactgc cagcggattt cgtgtcgata
     4081 acactaaatc gaccaaccct gcactaaaac cgaaaccggc aatccattcc attgaggctg
     4141 caatataaac agataatccg gttaataatg cgtgaaccac ataaagcaca ggtgcaacaa
     4201 acataaaggc aaattcaagc ggttccgtaa tgccggtgaa gaacgaagct aaagcagcag
     4261 caaacataat agaggctact tgcattttct tagccggttt cgcactgtgg taaatagcta
     4321 atgctgccgc aggtaagcca aacatcatca ccgggaagaa accagcttga tacatacctg
     4381 ttactcctat cactgctttg ccctcagcca atgattttgc cccacctaag aagtttggaa
     4441 tatcattaat gcctgcaaca tcaaaccaga aaaccgagtt taatgcgtgg tgtaatccaa
     4501 ccggaattaa taaacgatta aagaaagcat aaatacctgc ccctaacgcc cccatatctt
     4561 tgatagattc cccaaaagag actaaagcac caaaaataac cggccagaca taaagcaaga
     4621 taaatgacac cacaatcggc accaatcgtt taccgctaaa gaaagacaat gctttcggta
     4681 actctacttg gtagaaacgg ttataaattt cggctgaaat aacccctact aagatcccga
     4741 taaattggtt gttgatttta ccgaatgctg caggcacttg atcaactgcg atccctttta
     4801 gctgagcaac ggtccccgga gacaacaaag tggtaattac taaatacccg actaaaccgg
     4861 ataatgctgc tgccccatct ttttcttttg acataccaaa tgccacaccc acggcaaata
     4921 gaatagacat attatcaata attgccgccc cagatttaat cagcaatgcc gctaattggc
     4981 tatttgttcc ccagccgtcc ggatcaatcc aatagcctat acccatcaat aatgctgctg
     5041 ctggtaatgc agcaaccggc accattaatg cacgcccaat tttttgtaaa taggttaatg
     5101 tactcataaa agcctccaaa aatacctcat aatgagtaac tgaagcatag aaaaaaaccg
     5161 attaaagaac aatcaaataa atttggtttt gtgatccatt tcaaaatatt ttttaggagt
     5221 ttttattcaa cattagaaat atgtttactt tttatcgcta tattttaaat aattacctaa
     5281 atagcattgg attacctaaa tattcattga caaaacatta ataaaacggt atttctatgc
     5341 acacttttca atactgttga aaaagagtaa tgggcttgcc attacgggag aggtgcaaca
     5401 ctcaggcaac ttgattttag gcaactaggg ccgtttgaga attaagtgat gggagtgatt
     5461 gccgaggtgt gaattcacta gactggaatt aacatcggct gaataggttg aatcctatca
     5521 gctgtcattt aataggttat ttcaccatat taaatggagt gctctgatta attggttcaa
     5581 caataaaacc gtataatcta ctcaaatttc ctttccgaat atcccctaac acaatattta
     5641 tctatttttg ttttaaggaa atttttatta tgtctcatct ttctgtggct aaatttggtg
     5701 gtacaagtgt tgcaaatttt gatgcaatga cctcttctgc caatattgta attgctgatg
     5761 caaatactcg tgtggtggta ctttctgcct ctgctggcgt aaccaattac ttagtggaac
     5821 ttgctaacgg ctgtgaaaaa gaacaccgtg atgaagtcat cgcagcagtc cgccaaattc
     5881 aatacaatat tattgaaaaa ttacaacatc aagatgccgt taaagcggaa atcgacgaat
     5941 tattagaacg tattgaaaca ttagcagaat cagccagcct tgctacttca gctgccttaa
     6001 gtgatgagtt aatcagccat ggggaaatga tgtccaccaa aatcttcacg caattattaa
     6061 ttgaacgtgg acacccggca gtatgggtag atgtgcgtga cgttgtggca acaaacagcc
     6121 attatggaaa agcagcacca aatgacgaga aaacccagca acaatcagac aatgtgatta
     6181 agccattaat cgcatcaggc aaaattgtga ttacacaggg ctttattggt cgtgatgatg
     6241 aaggcaaaac cactacttta ggacgtggcg gttcagacta ctctgctgca ttgttagcag
     6301 aagtattaaa tgctaacgat gtactcattt ggacagacgt accggggatt tataccaccg
     6361 atcctcgtgt tgtaccgaac gctcaacgca ttgacacaat ggcatttaac gaagcggcag
     6421 aaatggcaac atttggagcg aaagtattac acccagcaac cttattaccg gcagtgcgta
     6481 gcaatatccc tgtatatgtt ggctcaagca aagcaccgga acaaggcggc acttgggtaa
     6541 cccgtgatcc acaaccacgc ccgactttcc gtgcgattgc attacgccgc aaccaaattt
     6601 tactcactct ttccagcctt tcaatgctac acgcacaagg tttcttagcg aatgtatttg
     6661 cgattttagc gaaacacaaa atttcggttg atgtgattac cacttccgaa gtcagcgttg
     6721 cattgaccct agataaaacg ggctcagcct cttcaggtgc agatatgtta tcagcggaat
     6781 tactcgatga gcttaacgca tattgcaatg tgcaagtaga aagcgacctc gctttagtcg
     6841 ctattatcgg caacaactta cacgctcagt ccggtgtggc gaaaaaatta ttccacactc
     6901 ttgagaatta caacattcgc ctaatcagct atggagcaag cagcaataat gtttgtacct
     6961 tagtgaaaaa tgcagaagca gacgatgtgg ttcgttcgct tcacaaaggc ttatttgaat
     7021 aagcaataac tccattgcaa gcggttattt ggggcaaaat tgttggattt accccctaac
     7081 cgcttcttct ctttactcaa caatctctac cgttgaattg gtactaagcc ctctcactag
     7141 ctgtgagcct tttcttgctc gttcgccacg gtagttatca atatcgctcg gttttaatgt
     7201 gattttgcgt ttaccggaca caaagaccag cgagttggtt ggtttgatga ccaataaacg
     7261 ggctaaatat tcatcaccgg attttgctgc ggcagcggaa atattgataa ttttattgcc
     7321 tttacctttg gaaagtgccg gtagttcact caccgggaaa accaacatcc taccgacatt
     7381 actcattgcc actaaagata aattttcatc attttccaat aattgcggcg gcagcacttt
     7441 tgcgttttcg gttaaagaaa taaccgcttt gccggcttta ttacgagaaa ccaaatcttc
     7501 aaaggtgcaa ataaagccgt agcctgaatc ggatgccatt agcactttgg tttccgaatt
     7561 tgccattaat acttgttgaa cagttgcacc ttcaggtaga gtgattttgc ctgtaagtgg
     7621 ctcaccttgc gagcgtgcag aaggcagtga agttggatcg agtgcataag ctcgcccagt
     7681 gctgtctaag aacaccacag gctgattact tctgcccctt gcgtgggcaa gataatcatc
     7741 tccggctcga tagctgaggg cttgaacatc aatatcatgt cctttggcac aacgtaccca
     7801 gcctttttca gacaaaatca ctgtcacatc ttcgattggg gttaagtcgc tttccgcaat
     7861 cgctttagac tccgctcgtt ccaccagagg tgagcggcgt gggctggcaa atgctttggc
     7921 atcggcttga atctctttct taatcaggct gttcaaacgg cgttccgagc ctaaaatcag
     7981 ttgcaattca tcacgttctt tttccagttc ggatttttcc gcctgtaatt gatgctcttc
     8041 caatttggct aagtggcgta aacgtaagtt caggatcgcc tctgcttgca catcggtcag
     8101 attaaaacga gccattaact cggctttcgg ctcatcttca ttgcgaataa tctcaatcac
     8161 ttcatcaata ttgagaaatg caatcatcaa accgtctaaa atatacaagc ggtttaaaat
     8221 tttatctaat cggtaattca ggcggcgagt gacagtggta cgtctaaaac tcagccactc
     8281 attgagaata gtcagcaaat tcttcactgc cggcttgccg tctaagccaa tcatattcat
     8341 attgactcgg tagcttttct ccaaatcggt cgtcgcaaac aggtgatcca ttaacgcatc
     8401 aaaatcaatg cgattagagc gtggcacaat cacaatgcga atcgggttct cgtggtcaga
     8461 ttcatcacga atatcgtcca ccatcggtag ttttttattc cgcatttggg ttgctacttg
     8521 ctcaataatt tttgaaggag aggcttggtg cggcaaggct gaaatcacaa tttcaccctc
     8581 ttcttttttc cacaccgcac gcattttaat agaaccacgc ccctgctcat acattctggc
     8641 aatttcc
//
LOCUS       DANKBV010000094         8624 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.094, whole genome shotgun sequence.
ACCESSION   DANKBV010000094 DANKBV010000000
VERSION     DANKBV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.094"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1301)
                     /locus_tag="PWY43_001775"
     CDS             complement(<1..1301)
                     /locus_tag="PWY43_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDL2136638.1"
                     /translation="MSRSKKVTEHLEHSAEKVVEEAQQAVDSAESFAKKEIEEDVVNE
                     AIEKDIQTEMQKAPKEQVVVQKSGGKGLALVALLVALAVGGAGHYMANKKFAEVESQI
                     QAVSAKANQPTTQTMVEMPNFENEKAQIAELVTEYQKALERIKALETAQNGYTQQISS
                     LQLQLQKLNNTSGSDNTFWLLSEADFLLNNAARKVVLDNDIETAKNLLLEADQVLSKA
                     PNTIHIREAIKTDLNTLTALHQIDQNALMQRVANLANKLDDLPILESEQASNSKQDGQ
                     VSDSLADWEKNLEKSAASFLDHFIRVSKRNAADEKAFVAPNQEIYLRENIRLRLQIAI
                     LAVPRQQSELYKKSLQTVGSWVRSYFETSNENVQHFLKELDELAEQTIYVDAPTSLQS
                     LKVIAQQINKTPEKIEKVEIKAEKELEQAEPVKAEQDAMPPS"
     gene            complement(1313..2077)
                     /locus_tag="PWY43_001776"
     CDS             complement(1313..2077)
                     /locus_tag="PWY43_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDL2136639.1"
                     /translation="MNVLVTRPDSRGQELVDMLNEQQIFAIHQPLFTIEAGKELPLLP
                     SAISRLKSDDYVFVVSPNAVDYAAKTLVDTGFHFRSDLKYFAVGQRTAKYLTEKAEQA
                     VIYPIEFENSEGVLALPEMQNLTDKTILILRADSGRELLAETAVLRGATIQYLECYRR
                     EPVTDDIPEKISLCKRLGVDTIVITSSEILRSLYEQAKADCRAWLFECDLVVVSRRIA
                     KIAKQMGWQEDKIRVSDKADNVTLMNTLLFKVGKSN"
     gene            complement(2077..3012)
                     /gene="hemC"
                     /locus_tag="PWY43_001777"
     CDS             complement(2077..3012)
                     /gene="hemC"
                     /locus_tag="PWY43_001777"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDL2136640.1"
                     /translation="MKDILRIATRQSPLALWQANFVKSELEKHFPQLSVELVTMVTKG
                     DIILDTPLAKIGGKGLFVKELELALLENRADIAVHSMKDVPMSFPEGLGLAVICERED
                     PRDAFVSNNYHSLEDLPKGAVVGTSSLRRQCQLMAKYPHLEVKSLRGNVGTRLSKLDN
                     GEYDAIILASAGLIRLGLQERIRSYISVEQSLPAAGQGAVGIETRANDERILNYIAKL
                     NHSSTACCVMAERAMNTRLQGGCQVPIGGYATLEGDELTLNALVGSLDGSQIIRASAK
                     GHKQEAEQLGILVAEQLLAQGADKILAAVYNENVQ"
     gene            3308..5917
                     /gene="adhE"
                     /locus_tag="PWY43_001778"
                     /gene_synonym="adhC"
     CDS             3308..5917
                     /gene="adhE"
                     /locus_tag="PWY43_001778"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL2136641.1"
                     /translation="MAKNTQPFDAQAEVNELVEKGLKALDEFRKLNQEQVDFIVAKAS
                     VAALDKHGILAMHAYEETGRGVFEDKATKNLFACEYVVNNMRHLKTAGVISEDDVTGI
                     TEIADPVGVVCGITPTTNPTSTTIFKALIALKTRNPIVFAFHPSAQQSSAHAAQIVRD
                     AAVAAGAPENCIQWITQPSMEGTSALMKHPGIATILATGGNAMVEAAYSCGKPALGVG
                     AGNVPAYVEKTAKLKQAVYDIVMSKSFDNGMICASEQAAIVDKEIYAEFIKEMQSYGV
                     YLVNKKEKALLEKYIFGIDKAKDENCSGAKLNSAVVGKPAAWIAEQAGFKVPEKTNIL
                     LAECAFVGEAEPLTREKLSPVLALLKSDSTEHGLELSEAMVNFHGLGHSAAIHTENKA
                     LAKTFGERVKAIRVIWNSPSTFGGIGDVYNSFLPSLTLGCGSYGKNSVGNNVSAVNLI
                     NIKRVGRRRNNMQWFKVPSKIYFERDSIQYLKSMHDVHKVMIVTDRSMVDLGFVDRIT
                     EQLRQRRNQVMIQLFTDVEPNPSLQTVQRGTELMRSFQPDTIIALGGGSPMDAAKIMW
                     LFYEQPEVDFRDLVQKFMDIRKRAFKFPQLGRKAKFVGIPTTSGTGSEVTPFAVITDG
                     DIKYPLADYSLTPTIAIVDPALVMSVPSHVAADTGLDVLTHATEAYTSILANDYTDGL
                     ALQAIKLVFENLEKSVKEFDETAREKMHNASTMAGMAFANAFLGICHSMAHKIGGKFH
                     TIHGRTNAILLPHVIRYNGTRPTKVATWPKYTNYVADVRFQDIARMLGLPAETPEQAV
                     EAYAQAVHDLAVRCGVKMSLREQGVDEQAFLNARRELALHAFEDQCTPANPRLAMIED
                     MEAIMTKAYYGKE"
     gene            6064..6960
                     /locus_tag="PWY43_001779"
     CDS             6064..6960
                     /locus_tag="PWY43_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDL2136642.1"
                     /translation="MQITILDGGISRELMRRNAPFHQPEWSAAALYEGPHFVQAVHED
                     FIAHGAEVITTDSYAVVPFHIGEKRFAADGKMLADLAGRLAKQAVKNSENFATQIAGS
                     LPPMFGSYRADLIQMERFAEVAQPLIDGLSPYVDIWLCETQSAIIEPISIKALLPKDN
                     RPLWVSFTLTDDEPTTEPQLRSGESVKQAVEEMVKLGVQAILFNCCQPEVIGEALSVT
                     QKTLAELKATHIRTGAYANAFAPQPKDATANDGLDEVRQDLTLEAYLGWAKKWTEQGA
                     TIIGGCCGIGVEYIETLAKNLK"
     gene            6970..8379
                     /locus_tag="PWY43_001780"
     CDS             6970..8379
                     /locus_tag="PWY43_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2136643.1"
                     /translation="MSNKKIGLISLTALVLSSMIGSGIFSLPQNMAAVAGAEAITIGW
                     LITGVGIIFLGLSFFFISRLRPDLDGGIYTYAREGFGELMGFMSAWGYWLCATIGIVG
                     YLTVAFEGLGVFTDNPNTVIFGQGNTLASFIGSSIIVWLVHALIAGGIKEAAFVNLIA
                     TFVKVAPLILFIGLGIWYFDVDLFNSDVKATALNNSVGDQVKSTMLITLWVFTGVEGA
                     SVLSAHAKKRSDVGLATVLGIIIALALYVAITILSLGILPRETIANMANPSMGPLLDA
                     MMGPTGKVIITACLIVSVLASYISWTMYSAEVPYRGSKNGAFPKILDKVNKNGTPINS
                     LWFTGFIVQFCLVLVFVFEQSYNTLLLISTSMILIPYFLIGAYLFKLAIQTNATWYIK
                     LTGFMAALYGLWIVYAAGLEYLLLSVVLYVPGILLFLYSNYKFNGRLTLSTAEKVILI
                     VLLVIFGYAVIELPALLAA"
     gene            8486..>8624
                     /locus_tag="PWY43_001781"
     CDS             8486..>8624
                     /locus_tag="PWY43_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 domain-containing protein"
                     /protein_id="HDL2136644.1"
                     /translation="MQTYQHKALCRVRTITLFLSLTKDKLQWETALNVAKAEFDLLVP
                     AI"
BASE COUNT         2425 a         1950 c         1699 g         2550 t
ORIGIN      
        1 gaaggaggca tcgcatcttg ctctgctttt acaggctctg cttgctctaa ttctttttca
       61 gcttttattt cgactttctc tattttttca ggggttttat taatttgctg agcaatcacc
      121 tttaagcttt gtaatgaagt tggagcatcc acataaatag tttgttctgc caactcatct
      181 agctctttca agaaatgttg cacattttca tttgaagttt caaaataact tcttacccaa
      241 gagcctaccg tttgtaacga ttttttataa agttcgcttt gctggcgagg aacagctaaa
      301 atcgcaattt gtaaacgcaa gcgaatgttt tcacgcaaat aaatttcttg attcggtgca
      361 acaaaggctt tctcatctgc tgcattacgt ttcgatacac gaataaaatg atctaaaaat
      421 gaagcagcac ttttttctaa atttttctcc caatccgcaa gagaatcact tacttgccca
      481 tcttgtttgc tatttgaagc ttgttcactt tccaaaatcg gcaaatcatc taatttattc
      541 gctaaatttg cgacacgctg cattaaggca ttttgatcga tctgatgcaa tgcggttaat
      601 gtatttaaat cagttttgat tgcctcacga atgtgaatcg tattcggtgc ttttgataaa
      661 acttgatctg cctctaacaa taaatttttc gctgtttcaa tgtcattatc taacaccact
      721 ttacgagccg cattattcaa taggaaatct gcctcagata atagccagaa tgtgttatcc
      781 gaaccagagg tattatttag cttttgcagt tgaagctgca aactactaat ttgctgggta
      841 taaccatttt gggctgtttc taaagcttta atccgttcta aagctttttg atattccgtg
      901 accaattcgg caatttgtgc tttttcattt tcgaaatttg gcatctcaac catagtttga
      961 gtagtaggct gatttgcttt agcagatact gcctgaattt gagattcaac ctctgcaaac
     1021 tttttatttg ccatataatg accagctccg ccaaccgcta aagccactaa tagtgcaact
     1081 aatgccaagc cctttccgcc cgatttttga acaaccactt gttcttttgg cgctttttgc
     1141 atttctgttt gaatatcttt ctctatagct tcgttcacta catcttcctc aatctctttt
     1201 tttgcaaaag attccgctga atcgaccgct tgttgtgcct cttctaccac cttttcagct
     1261 gaatgctcta aatgttcagt tactttttta gatcttgaca tactcgccac ctctagttag
     1321 atttgcccac tttaaataat aatgtattca tcaaagttac attatcggct ttatccgata
     1381 ccctaatctt atcttcttgc cagcccattt gtttagctat ctttgcaatc cgccggctca
     1441 ctacaaccag atcacattca aataaccatg ctctacaatc tgctttcgct tgttcataaa
     1501 gtgatcttaa aatttcacta ctggtgatca caatagtatc tactcccaag cgtttacata
     1561 agctgatttt ttcgggaata tcatcggtaa ccggctctct acgataacac tccaaatatt
     1621 gaatagttgc cccacgcaag actgcagttt cagcaagtag ctcacgccca gaatcggctc
     1681 ttagaattaa aatagtttta tcagttagat tctgcatttc aggtaatgcc aaaaccccct
     1741 cgctattttc aaattctatc ggataaatga ccgcttgttc agctttttct gttaaatatt
     1801 tcgcagtccg ttgccctacc gcaaaatatt tcaaatctga acgaaaatgg aacccggtat
     1861 ctactaatgt ttttgcagca tagtccactg cattcggcga aacaacaaat acataatcgt
     1921 ctgattttag ccgagaaata gccgaaggaa gcaaaggtaa ttctttaccg gcttcaatcg
     1981 taaatagcgg ttgatgaata gcaaaaattt gctgttcatt caacatatct accaattctt
     2041 gcccccgact atccgggcgt gtgaccaaca cattcattat tgtacgtttt cgttataaac
     2101 cgcagctaaa attttatctg caccttgagc aagcaactgt tccgccacca aaatgcctaa
     2161 ttgctccgcc tcttgcttat gtccttttgc cgaagcccga ataatttgtg agccatctaa
     2221 cgatccaact aacgcattaa gcgttaattc atcgccctct aatgtagcat aaccaccaat
     2281 cggcacttgg caaccgcctt gcaaacgggt attcattgca cgttctgcca tcacgcaaca
     2341 agcggtcgaa ctatggttta attttgcaat gtagttcaaa atccgttcat cattggctcg
     2401 agtctcaatc cctactgctc cttgaccagc tgcgggtaat gattgttcca cagaaatata
     2461 gctacggatt cgctcttgca agcctaaacg aattaagccg gcagaggcta aaataatcgc
     2521 atcatactcg ccattatcta atttgcttaa gcgagtgccg acattgcctc gcaatgattt
     2581 aacctctaaa tgcggatatt tcgccattaa ttggcactga cggcgtaagc tcgatgtacc
     2641 gaccacagca cctttcggca aatcttctaa cgaatggtag ttgttcgaca caaacgcatc
     2701 acggggatct tcacgttcac aaatcaccgc aagccccaag ccttcgggaa agctcatcgg
     2761 cacatctttc attgaatgca ccgcaatatc agctcgattt tctaaaaggg ctaattctaa
     2821 ctctttcaca aacaagcctt ttccgccaat tttggctaat ggggtgtcta aaataatatc
     2881 gcctttcgtt accatcgtaa ccagttcgac agataattgc ggaaaatgtt tttctaattc
     2941 gctttttaca aaatttgcct gccataaggc taacgggctt tgacgggttg caatgcgtaa
     3001 aatatctttc ataaattcta aaattaaaac attgagttaa tgaattatcc taccactttt
     3061 tagctgaact gccaaaaata atcgtggatt tcgctttgca tttaatcaaa aatccggctt
     3121 tttctcaaaa tgtatcaata tctatatgac taaatatttt atagcatata tacaacgtta
     3181 aaaaagttga tatagatcaa agcaaacata aaacattatt aatttttaac gacaaaataa
     3241 ctcttttggg taataatttc atcagttaaa aaaattatca aatttaaatt ttatgaggaa
     3301 ccaaactatg gctaaaaata cgcaaccatt tgatgcccaa gcagaagtga atgagcttgt
     3361 tgaaaaaggc ttaaaagccc ttgatgagtt tagaaaactc aaccaagaac aagtggattt
     3421 tattgtcgct aaagcctctg ttgctgccct tgataaacac ggtattttag caatgcacgc
     3481 ttacgaagaa accggacggg gtgtgtttga agacaaagcc actaaaaacc tgttcgcctg
     3541 tgagtatgtt gtcaataata tgcgacattt gaaaaccgca ggggtcatta gtgaagatga
     3601 tgtcaccggt attaccgaaa ttgccgaccc tgtcggtgtt gtctgcggta tcaccccaac
     3661 caccaaccca acctcaacca ccattttcaa agcgttaatc gcccttaaaa cccgtaaccc
     3721 tatcgttttt gctttccacc cgtctgctca acaatcttct gcccacgctg ctcaaattgt
     3781 gcgtgatgcc gccgtggctg ccggtgcacc ggaaaactgt attcaatgga ttacccagcc
     3841 ttcaatggaa ggtacatctg ctttaatgaa acacccgggc attgcaacta ttttagccac
     3901 cggcggtaat gcaatggttg aagccgctta ctcttgcggc aaaccggcat taggagttgg
     3961 tgcaggcaac gtaccggctt atgtagaaaa aaccgctaaa ttaaaacaag cggtgtatga
     4021 cattgtaatg tcaaaatctt tcgacaacgg tatgatttgt gcctctgaac aagcggcgat
     4081 tgtagataaa gaaatttacg ctgagtttat taaagagatg caatcttacg gcgtctattt
     4141 agtgaacaaa aaagagaaag ccttattgga aaaatacatt ttcggtattg ataaagccaa
     4201 agatgaaaac tgctcaggtg caaaactcaa ttcagcggtg gttggcaaac cggctgcgtg
     4261 gattgcagaa caagccggct ttaaagtacc ggaaaaaacc aatattctgt tagccgaatg
     4321 tgcctttgtg ggcgaagctg agccacttac ccgtgaaaaa ctttcgcctg tactggctct
     4381 gttaaaatcc gactccaccg aacacggttt ggaattgtct gaagcgatgg tgaatttcca
     4441 cggtttaggg catagtgccg ccattcatac cgaaaacaaa gccttagcca aaacctttgg
     4501 cgaacgggta aaagccattc gggtgatttg gaactcacca tcaacctttg gcggtatcgg
     4561 tgatgtatat aactccttcc tgccatcgct tacgctgggc tgtggttcct acggtaaaaa
     4621 ctctgtaggt aacaacgtta gtgcggtaaa tttaatcaat atcaaacgtg tgggcagacg
     4681 gagaaataat atgcaatggt ttaaagtgcc atcaaaaatc tactttgagc gtgactcaat
     4741 tcaatatttg aaatcaatgc acgatgtgca taaagtaatg atcgtaaccg accgttcaat
     4801 ggttgatctc ggctttgtgg atagaattac cgaacaactt cgccaacgtc gcaaccaagt
     4861 gatgattcag ctctttaccg atgttgaacc aaacccaagc ctgcaaaccg tacaacgtgg
     4921 tacagaatta atgcgtagct tccagcctga tactatcatc gcacttggtg gtggttcacc
     4981 aatggatgca gccaaaatta tgtggttatt ctacgaacaa ccggaagtcg atttccgtga
     5041 tttagtgcaa aaatttatgg atatccgcaa acgtgcattc aaattcccac aattaggcag
     5101 aaaagcgaaa tttgtcggca ttccaaccac ctccggaaca ggctctgaag tcacaccgtt
     5161 tgcagtaatt accgatggcg atattaaata tccgctcgct gactactcgc ttaccccaac
     5221 cattgcgatt gtcgatcctg cgttagtaat gtctgtgccg tctcacgttg ctgccgacac
     5281 cggtttagac gtattaactc acgctaccga ggcttacaca tcgattttag ccaacgacta
     5341 taccgacggc ttagccttgc aagccattaa gttagtgttt gaaaacttgg aaaaatcggt
     5401 caaagagttt gatgaaaccg cccgtgaaaa aatgcacaat gcttccacaa tggcagggat
     5461 ggcatttgcc aatgccttct taggtatttg ccactcaatg gcacacaaaa tcgggggtaa
     5521 attccacacc atccacggtc gaaccaatgc gattttactg ccacacgtta ttcgctataa
     5581 cggcacacgc ccaaccaaag tggcaacttg gccaaaatac accaattatg tcgcagatgt
     5641 gcgtttccaa gatattgcca gaatgcttgg cttaccggca gaaaccccgg aacaagcggt
     5701 ggaagcttac gcccaagcgg tacacgattt agccgttcgt tgcggcgtga aaatgtcgct
     5761 tcgtgagcaa ggcgttgatg agcaagcctt cttgaatgcc cgccgtgaac ttgccctaca
     5821 cgcatttgaa gaccaatgca caccagcaaa cccacgtttg gcaatgattg aagatatgga
     5881 agcgattatg actaaagcct actatggcaa ggaataaagc acaatttaat cgttaaactt
     5941 actaaatggc cgcttgcaag cggtcatttt tcttgaaatt tttacttttc ccttcacaaa
     6001 atactttcgc tcttttacaa agcatagtat ttttatcgct ccttttatct tacaggaaat
     6061 actatgcaaa ttacgatttt agacggcgga attagccgtg aattaatgcg tcgcaatgct
     6121 ccatttcatc agcctgaatg gtctgctgcc gctttgtatg aaggcccaca ttttgtgcaa
     6181 gcggtacacg aagattttat cgctcacggg gcagaagtga ttactaccga cagctatgcg
     6241 gttgtgccgt ttcacattgg cgaaaaacgc tttgcggcag acggcaaaat gcttgccgat
     6301 ttagccggtc gccttgccaa acaagcggtc aaaaattcgg aaaatttcgc aactcaaatt
     6361 gcaggctcac ttccaccaat gtttggctct taccgtgccg atttaattca aatggaacgc
     6421 tttgctgaag tcgctcagcc tttaattgac gggctttcgc cttatgtgga tatttggctt
     6481 tgcgaaaccc aaagtgcgat tattgagccg atttccatca aagcattatt gccgaaagac
     6541 aaccgcccgc tttgggtctc ttttaccctc accgatgatg aaccaaccac cgagccacaa
     6601 cttcgctcgg gcgaaagcgt taaacaagca gtcgaagaaa tggtgaagtt aggcgtgcaa
     6661 gcgattttat ttaactgctg tcagcctgaa gtgatcggcg aggcattaag tgtaacccaa
     6721 aaaactttag ccgaattaaa agctacccat attcgcacag gagcttatgc caacgccttt
     6781 gctccacaac caaaagatgc caccgccaat gacggtttag atgaagttcg ccaagattta
     6841 accctggagg cttatttagg ctgggcaaaa aaatggacgg aacaaggggc aactattatc
     6901 ggtggctgct gcggcattgg ggttgagtat attgaaacgc tggcaaaaaa tttaaaataa
     6961 ggaaatcgaa tgtcaaataa aaaaatagga ttaatttcac tcaccgcctt agtgctaagc
     7021 tcaatgattg gctcggggat tttcagctta ccgcaaaata tggcagccgt tgccggagca
     7081 gaagccatta ccattggctg gctgattacc ggtgtcggca ttatcttttt aggtttatcg
     7141 tttttcttta tttcccgctt gcgtccggat ttagacggtg gcatttacac ctacgcacgg
     7201 gagggttttg gcgagctgat gggctttatg tcagcgtggg gctattggct ctgtgccacc
     7261 attgggattg taggatacct caccgttgct tttgaaggtt tgggtgtatt taccgacaac
     7321 ccgaacaccg tgattttcgg gcaaggtaac accctcgcct cttttattgg atcttcaatc
     7381 attgtgtggc tggttcacgc cctcatcgca ggcggtatca aagaagctgc ctttgtaaac
     7441 ttaattgcca cttttgtgaa agtcgctccg ctcattctgt ttatcgggtt aggcatttgg
     7501 tatttcgatg tggatctgtt taatagcgat gttaaagcca ccgcacttaa taatagtgta
     7561 ggcgaccaag tgaaaagcac aatgttaatc accttatggg tatttaccgg tgtggaaggg
     7621 gcttctgtgc tttcggctca cgcgaaaaag cgttctgacg tgggcctggc aaccgtatta
     7681 ggtattatta ttgcacttgc tctttatgtg gcaattacca ttctttcact cggaattctg
     7741 ccacgggaaa ctattgccaa tatggcaaat ccgtcaatgg ggccgttgct cgatgcaatg
     7801 atgggcccaa ccggcaaagt gattattact gcttgtttaa ttgtatcggt gcttgcctct
     7861 tacattagct ggacgatgta ttcggcagaa gtgccttatc gtggctcaaa aaacggggct
     7921 ttcccgaaaa ttttagacaa agtgaataaa aacggcacgc cgattaactc actctggttt
     7981 accggcttta tcgtgcaatt ttgcttggtg ttggtgtttg tttttgagca aagctataac
     8041 accttattgc tgatttccac ctcgatgatt ttaattccct atttcttaat cggggcttat
     8101 ttattcaaat tagcgattca aaccaacgcc acttggtaca ttaaactcac cggctttatg
     8161 gcagcccttt acggattatg gattgtctat gccgcaggac tagaatattt gctgctttcg
     8221 gtggtgcttt atgtacccgg aattttactc ttcctctact ccaactataa attcaacggc
     8281 agattaaccc tttcaaccgc tgaaaaggtc atcttaatcg tgctactggt aatttttggc
     8341 tatgccgtga ttgagctgcc agcactatta gcagcataat tcccatcaaa aatgaccgct
     8401 tgcaagcggt tatttttcta ggatcttttg caaagattca ttagaataga aaaaacgtca
     8461 ataataaata aaaagaggag aaacaatgca aacttatcaa cataaagccc tttgtcgggt
     8521 aagaaccatt accctttttc tgagtttaac caaagataaa ttgcagtggg aaacggcatt
     8581 aaatgtggca aaagcagaat ttgatctatt agttcccgct attc
//
LOCUS       DANKBV010000095         8591 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.095, whole genome shotgun sequence.
ACCESSION   DANKBV010000095 DANKBV010000000
VERSION     DANKBV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.095"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            127..1491
                     /gene="mpl"
                     /locus_tag="PWY43_001782"
     CDS             127..1491
                     /gene="mpl"
                     /locus_tag="PWY43_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDL2136645.1"
                     /translation="MKKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFL
                     AAHGIEIIPNYDVAQLQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLL
                     KDKWVLAVSGTHGKTTTTGMLAWILDQNGIDTGFLIGGVAGNFGISARAGNSPFFVIE
                     ADEYDSAFFDKRSKFVHYTPKTLIINNMEFDHADIFDDLKAIQRQFHHLVRTMPQSGC
                     ILSAIADQNVQDTLKMGSYSQLQFIGEQNEWFAKPLNADCSQFEVFHLGVKQGEVHWS
                     IIGKHNMHNALMAIAAAHHAGVEVQGACEALGKFVNANRRLEVKGEVNGITVYDDFAH
                     HPTAIEATIDALRGKVGKNQRILAVLEPRSNTMKMGVHKDEIAPSLHDAEAVFVYQPD
                     IIAWSVSEITNVLSQPAYWSANIDDLVDLVVKEAKPTEHILVMSNGAFGGIHQKLLAK
                     LADK"
     gene            1651..2640
                     /locus_tag="PWY43_001783"
     CDS             1651..2640
                     /locus_tag="PWY43_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL2136646.1"
                     /translation="MNDLIIYNTEDGKSNVALLVVENEAWLTQNQLAELFDTSVQNIS
                     EHIKNILKDKELDENSVIKDYLITAQDGKNYQVKHYSLEMILAIGFRVRSPRAVQFRK
                     WANSTLRTYLEKGFLIDSERLKNPQGRLDYFDELLEQIREIRASELRFYQKVRELFKL
                     SSDYDKTDKNTQMFFAETQNKLIYAITKHTAAELICQRADATKMNMGLTSWKGKVVRK
                     GDIVIAKNYLDNNELDSLNRLVMIFLESAELRVKNNQDLTLGFWRRNVDMLIEFNGFS
                     VLGNGGTITHKQMESFVREQYEKFDIQRKCLKQKEADWEDLQALEKLESELTR"
     gene            complement(2688..3842)
                     /locus_tag="PWY43_001784"
     CDS             complement(2688..3842)
                     /locus_tag="PWY43_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HDL2136647.1"
                     /translation="MNQFFPLIFSLTLFSGVTLALEKAPRTMPTEGINKPLLGQILFF
                     DNMLSHNGSQNCSSCHNPDYAFIDNRTTPAKGVVSQGDTPTKFGKRNSPTMLYAKYSP
                     EFHFDEQSQEYIGGQFWDGRAKNLAEQAGMPPIDPNEMGMPNKLEVAKRLYNVPMYQR
                     FLGQFYGEKVWESYESVYAAMEDAIATFQQENKLLAPFDSKYDLFLKGEVQLTALEEK
                     GRALFFNKAQTNCSSCHQLQSAANHQEETFSNYHYYNVGVPGNPDLIAVNGFAPDFVD
                     LGLFENPNVKGDETQKGKFKTPTLRNVAVTAPYMHNGVFKELKTVLLFLDSYNNPERK
                     INPETGTAWQQAEFEPTIAHQKLKAKPLSDEEIDALKAFLKTLTDERYRK"
     gene            4206..4392
                     /locus_tag="PWY43_001785"
                     /pseudo
     CDS             4206..4392
                     /locus_tag="PWY43_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
     gene            4621..6741
                     /locus_tag="PWY43_001786"
     CDS             4621..6741
                     /locus_tag="PWY43_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL2136648.1"
                     /translation="MGARATSANAIALGTDTAATGNRATAFGAGALATGNRSTVMGWR
                     SAASGTRSFAMGSGATANADFSGAMGDKSVANATYSLALGSSANATSTATRGTAIGYN
                     ATVSGVNATALGVSAVSSNQSTVAIGDSAKASAGFAIALGSGANAALNNSVALGSNST
                     VSNAVAVPSATVNGITYSGFAANTTAAGNVVSVGSNTIKRQIQNVAAGQNSATSTDAI
                     NGSQLYMAMNATGNLANSTKNILGGNATVNPDGSVTYTNIGGTNKNTIEEALKAVKTE
                     VVAGSNVNITNATGANGQTIYTVNAYNTTASSSSPDYITVTGKAATAANTTNYEIGLT
                     KKAIDDFTKDTQATVVSNDGTVTVKSTERNANGTVIYDLSVNIPAQASQIQYFSVNST
                     VPENQANDGAKSRNSIAIGPNATATGGEQAAVALGTNSNANGNGALSLGVVTVSKGIQ
                     ATAVGHSANATANGTTALGRQTNATAGDATAVGSNANATAEKASAFGVAANASANASL
                     AVGANSIASAQSAVAVGTRANATAQFATALGMGAQATLNSSVALGSESVVRAATPTEN
                     ATVGGITYNGFAGVNKDTNYVVSVGSAGKERQIQNVAAGQISATSTDAINDSQLYMAM
                     NATSNLANSTANNFGGGSVVNPDGSVTQPKYNVTNAAGTQYGNTATNVGDAITNLNNY
                     VNQGFNIKDNAGETKGTVTPNESV"
     gene            6814..7299
                     /locus_tag="PWY43_001787"
     CDS             6814..7299
                     /locus_tag="PWY43_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136649.1"
                     /translation="MDTGSITVDNTTGAVTTPAEEDKVATTKTVSEAIQKAGWNAKSG
                     GNKADSDQEAAELINPGEEVIFAAGDNLKVKRVGTTFTYETAKDVKFDSVTFGDNGPK
                     ITNKDGNVNIAGNDGNPTKITGVKAGEADTDAVNVSQLKQAAASQNRSERFRFSNCWC
                     T"
     gene            7262..>8591
                     /locus_tag="PWY43_001788"
     CDS             7262..>8591
                     /locus_tag="PWY43_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL2136650.1"
                     /translation="MKGSGLATVGAPETGDNGQTIYTVDVAKAGAPTVTRGNVAVKEG
                     DENKVMTAGDVADAINNSEKTSSVVAGSKAVTVKAGKEDDKGNTEYTVDVATDKSIGR
                     DNDGNMTVNTDNVTIVKDPTTGEVKANTTTLNNSPEGKVTEPTGDDAKKLVTAGDIAN
                     AINNSGFNVTAGDGVDGGETKGKKTQLIKPSETVTFDAGKNMTLTQADGKFTYTTKDN
                     VAFNSIDMSNGKPDTTGSITNLKSGVGGTFADKAAPTDAERKAIADNINNATGDTLNN
                     AVNVGDVQAATTKVEGDKGVTVTSKTNDDGSTTYTVEAKTDGTTIKVNDKGEITANTS
                     ELTNNPDGKVEEPTEPNALVTAKTVADAINNAGFNIQANGDEKSLVKTGDTVQFLNGK
                     NIEITRDGNITVSTAKNVNFDSVQFGNEGPKITNNGGNINVGDKDGNAVKV"
BASE COUNT         2697 a         1563 c         1934 g         2397 t
ORIGIN      
        1 ttacaatatt aagcctaaaa atgaccgctt acccctctcc ctgatttttt cttctgcacg
       61 aagaaaaaat ctatccctct cccacaaggg gagaggggat gattctaaca ttttaaatat
      121 aaaaagatga aaaaacatat ccatatttta ggcatttgcg gcacctttat gggcggtgtg
      181 gcaatgattg cccgtgagct tggctataaa gtgaccggct cagacactaa tgtttaccca
      241 ccgatgagta cctttttagc tgctcacggt attgaaatta tcccgaatta cgatgttgct
      301 caactgcaac cggctcctga tttagtggtg attggtaatg cgatgagccg tggtaatccg
      361 tgcgtggaat atgtgttgga caaccaactc aattacacct ccggcccgca atggttgcac
      421 gatcatttgc ttaaagacaa atgggtgctg gcggtttccg gcacgcacgg caaaactacc
      481 acaacaggaa tgttagcgtg gattttagac caaaatggga ttgataccgg ctttttaatt
      541 ggcggtgttg ccggtaattt cggcatttcg gctcgtgccg gtaacagtcc attcttcgtg
      601 attgaggcgg acgagtacga ttctgcgttc tttgataagc gttctaaatt tgtgcattac
      661 accccgaaaa cgctgattat caataatatg gaatttgacc acgccgatat ttttgatgat
      721 ttaaaagcga ttcaacgcca gttccaccac cttgttcgca cgatgccaca aagtggctgt
      781 attttgtcgg caatcgctga tcaaaacgtg caagatacac taaaaatggg cagttacagc
      841 cagcttcaat ttattggcga acagaacgaa tggtttgcta aaccgttaaa tgccgattgc
      901 tcacagtttg aggtgtttca cttaggcgtt aaacaaggcg aggtgcattg gtcgattatc
      961 ggcaagcaca atatgcataa tgctctgatg gcgattgccg ccgctcatca cgcaggggtt
     1021 gaagtgcaag gggcttgcga ggcattaggc aagtttgtga atgctaaccg ccgtttggaa
     1081 gtgaaaggcg aggtaaacgg cattaccgtt tatgatgatt tcgctcacca cccaactgcg
     1141 atagaagcga ccattgatgc tctgcgtggt aaagtcggca aaaatcaacg cattttggca
     1201 gtgttagagc ctcgttcaaa tacaatgaaa atgggtgtcc ataaagatga aatagcccca
     1261 agcctacacg atgccgaagc agtgtttgtt taccagcctg atattattgc gtggagtgtg
     1321 agcgagatta ccaatgtgtt atcgcaaccg gcgtattggt cggcaaatat tgatgattta
     1381 gtcgatctag tcgtcaaaga agccaaaccc accgagcaca tcttagtgat gagcaacgga
     1441 gcctttggcg gtattcatca aaaactactt gccaaattag ctgataagta acctttacaa
     1501 gcggtaaatt ttttacaaaa ttttaccgct tgtttgtata actctctatt tttgtgatct
     1561 gcatcgaaaa attcaaaatc aattctggat atttatacat gttttattga aaatagcgac
     1621 taatatctct accaataaac agaataaaat atgaacgatt taattattta taatacggaa
     1681 gacggcaaaa gcaatgtagc cttgttagtg gttgaaaatg aagcttggct gacacaaaat
     1741 caacttgcag aactttttga cacctctgtg caaaatatat ccgaacacat taaaaatatc
     1801 ttaaaagaca aagagttaga tgagaattca gttatcaagg attacttgat aactgctcaa
     1861 gatggcaaga attatcaagt aaaacattat tcacttgaaa tgattctggc gatagggttt
     1921 cgagttcgta gcccaagagc agtacagttt agaaaatggg caaacagcac attacgcact
     1981 tatctcgaaa aaggtttttt aattgatagc gagcgtttga aaaatccgca gggtcgttta
     2041 gattatttcg atgaattact tgaacagatt cgtgagattc gggcaagtga attgcgtttc
     2101 tatcaaaaag tacgagagct tttcaaactc tctagcgatt acgacaaaac agataaaaat
     2161 acgcaaatgt tttttgcaga aacgcaaaat aagctcattt atgcaataac caaacacact
     2221 gccgctgagt tgatttgtca acgtgctgat gcgactaaga tgaatatggg attaactagc
     2281 tggaaaggaa aagtcgtgcg taaaggcgat attgtgattg cgaaaaacta tttagataat
     2341 aatgagctgg attctttaaa tcgcttggta atgatctttc ttgaaagtgc cgaattacgt
     2401 gtgaagaaca accaagatct tacgttaggt ttctggcgaa gaaatgtaga tatgttgatt
     2461 gagtttaacg gcttttctgt attaggaaat ggtggaacaa tcacgcataa acaaatggaa
     2521 agctttgtca gagagcaata cgaaaaattt gatattcagc gtaaatgttt aaaacagaaa
     2581 gaggcagatt gggaagattt gcaggcattg gaaaagttag aaagtgagct aacacggtag
     2641 ttaaacgcaa gcggtaaaat ttccccaaaa ttttaccgct tgtagcctta ttttctatac
     2701 cgctcatccg tcaaggtttt taaaaatgct ttgagggcat caatttcttc atcgcttaag
     2761 ggtttggctt tgagtttttg gtgggcgata gtgggttcaa attctgcttg ttgccacgcc
     2821 gtgccggttt cagggttgat ttttcgctcc gggttattgt agctgtctaa aaatagcaaa
     2881 acagtcttta attctttaaa cacaccgttg tgcatataag gtgcagtcac ggcaacatta
     2941 cgcaatgttg gggttttgaa tttgcctttt tgtgtttcat cacctttaac atttgggttt
     3001 tcaaataagc ctaaatcgac gaagtctggt gcgaagccgt taaccgctat caagtctggg
     3061 ttaccgggta cgcctacatt gtaatagtga tagttgctaa aggtttcttc ttggtggttt
     3121 gcagcactct gtaattggtg gcaactacta caattagttt gagctttatt gaaaaataaa
     3181 gctctgcctt tttcttccaa tgcagttaat tgtacctcgc cttttaaaaa tagatcatat
     3241 tttgaatcaa atggagcgag aagtttgttt tcttgttgga atgtcgcaat cgcatcttcc
     3301 attgccgcat aaacgctttc ataactttcc catacctttt caccgtaaaa ttgccctaaa
     3361 aatcgctgat acatcggcac attatataat cttttggcta cttctaattt gttaggcatt
     3421 cccatttcat ttgggtcgat aggcggcata ccggcttgct cggcgagatt tttggctcgt
     3481 ccgtcccaga actgcccgcc gatatattct tgactttgct catcaaagtg gaactcaggc
     3541 gagtatttcg cataaagcat ggtcggggag ttgcgtttgc cgaatttggt tggagtatct
     3601 ccttgtgaaa ccaccccttt tgccggagta gtgcggttat caataaaggc ataatccgga
     3661 ttatggcagc tggagcagtt ttgtgaaccg ttgtgtgaca gcatattgtc aaagaataga
     3721 atttgtccaa gtaggggctt atttattcct tctgtcggca tagttcgagg tgctttttct
     3781 agtgccaaag ttacgccgga aaatagagtt aagctaaaga ttagagggaa gaattggttc
     3841 ataaaagcat acctttttaa cgataactac agtgataatt caatagtgtt gaacgattat
     3901 ccgtaaacct tagcttattt taaaatttca ctgcgtataa atctcccctc aaaatgaggt
     3961 agtggcttga tttaagtcaa ggtgttttgc gttataacaa gcggttagat tcctcgtatt
     4021 atttacaata aatttacact ccagaagatg aattatatta caaatagcgt aaaatacgcg
     4081 cacttaaatt tctgagtcta taagtaagtt atttcactta aataggtggc ggttttttat
     4141 attggtgaag attatgttga acataggttc tacgtaatct tttttatttt tatatagggt
     4201 cgattatgaa tcaaattttt aaagttattt ggaatcaggc aacacaaagt tgggttgcag
     4261 tatctgaatt acaacgagcg aaaggtaaaa cgatgcaagt aaggcaacat tggctttatt
     4321 ggctggagct agtgccgtgt tgggagctaa tgatgctgtg gctgttgtat ctataggttc
     4381 aacaacaagt aattatattt caacacaagc aactgcatca tcggagaacg caattgctat
     4441 tggtgcggat tcaatctcaa ctggcagatc tactattgca attggtacag atgcgaatgc
     4501 aacgcataga acaaatttag tggatccaga agatacttct tacattacta taatattggt
     4561 gtagagagtc tcaataatac tggtcagaat tgggcattag ttccttctat tgctattggt
     4621 atgggggcta gagcgacttc agcaaatgct attgcattag gtacggatac tgctgcaaca
     4681 ggtaaccgtg caacagcttt tggtgcagga gctctagcta ctggtaaccg ttcaacagta
     4741 atgggatgga gatctgccgc tagtggcact cgttcatttg caatgggttc aggtgcaacc
     4801 gcaaatgctg atttttcagg tgcaatgggg gataagtctg ttgcaaatgc tacttattct
     4861 ttagctttag gttcaagtgc taatgcaact tctactgcaa ctagaggtac agcgattggt
     4921 tataatgcga cagtttcagg tgttaatgcg acagctttag gggtaagtgc agtatcttcc
     4981 aaccaatcta cggttgcaat tggtgatagt gctaaagcaa gtgccggttt tgctattgcc
     5041 ttaggctcag gtgctaatgc agcgctaaat aattcggttg ctttagggag taattcaacc
     5101 gtttccaacg ctgtggcagt tccttctgcg acggtaaatg gtattactta ttccggcttt
     5161 gcggctaata ctacggcagc agggaatgtt gtttctgttg gctcaaacac gatcaaacgc
     5221 caaatccaaa acgtagcggc aggtcagaac tctgctacat caaccgatgc aattaatggt
     5281 tcacagcttt atatggcaat gaacgcaaca ggtaaccttg ctaattcaac taaaaatatt
     5341 ttaggtggta atgctacagt aaatcctgat ggttctgtta cctacactaa tattggtgga
     5401 acaaataaaa atacgattga agaagcacta aaagcggtaa aaacagaagt tgttgcaggt
     5461 agcaatgtta atattacaaa tgcaaccggt gcaaatggac aaactattta tacagtaaat
     5521 gcgtataaca ccacggcaag ttcttctagc cctgattaca ttactgtaac aggtaaagct
     5581 gcaactgctg cgaatacaac taactatgaa attggtttaa ctaagaaagc cattgatgac
     5641 tttacgaaag atacccaagc tactgttgtt tcaaacgatg gtacagtaac tgtaaaaagt
     5701 acagagagaa atgcaaatgg cacggttatt tatgatcttt ctgtcaatat tcctgcgcaa
     5761 gcaagccaaa ttcaatattt ctctgtgaat tcaaccgtac ctgagaatca agctaatgat
     5821 ggcgcgaaga gcagaaactc aattgcgatc ggcccgaatg caacggcaac aggtggagag
     5881 caagcggcag tagcattagg cacaaactca aatgcaaatg gtaacggagc gttatcttta
     5941 ggtgttgtta cggtttcaaa aggtattcaa gctacggcag ttggtcattc agcaaatgca
     6001 acggctaatg gtacaaccgc acttggtcgc caaacaaatg cgacagcagg tgatgctaca
     6061 gcggtaggtt ctaatgctaa tgcaacggct gaaaaagctt ctgcctttgg tgttgcagca
     6121 aatgcgagtg caaatgcctc tttagccgta ggtgcaaatt caattgcctc agcacaatca
     6181 gcagtggctg ttggtacacg tgctaatgca acagctcaat ttgctacagc attaggtatg
     6241 ggagctcaag ctactctgaa tagttctgtt gctttgggta gcgaatcagt cgtaagagca
     6301 gcaacaccaa ctgaaaatgc aacggtaggg ggtattactt ataatggctt tgccggggta
     6361 aataaagata ctaactatgt tgtttctgta ggctctgcag gtaaagagcg tcaaatccaa
     6421 aatgtggcag cgggtcagat ttctgctaca tcaaccgatg cgattaatga ttcacaactt
     6481 tatatggcaa tgaatgcaac cagtaacctt gcaaattcaa ctgcaaataa ttttggtggc
     6541 ggttctgttg tgaatccgga tggctctgtg actcagccta aatataatgt aacgaatgca
     6601 gctggaactc agtatggtaa tactgcaaca aatgttggcg atgcaattac gaacttaaat
     6661 aactatgtaa accaaggttt caatattaaa gataatgcag gtgaaaccaa aggcacagtg
     6721 accccaaatg aatctgtata atttgtaaac ggtaaaggta cagtttcaaa tgttacacaa
     6781 gaagctgatg gcgtaactaa agtaaccttt gatgtggata ccggttctat cacggttgat
     6841 aacacaacag gtgcagtaac tacacctgca gaagaagata aagttgcaac tacaaaaaca
     6901 gtttctgaag cgattcaaaa agcaggttgg aatgcaaaat ctggtggcaa taaagctgat
     6961 agtgatcaag aagcagcaga gttgattaat ccgggcgaag aagtgatttt tgcagcaggc
     7021 gataacttaa aagttaaacg tgttggcact acattcacgt acgaaacagc gaaagatgtg
     7081 aaatttgata gcgtaacatt tggtgataat ggcccgaaaa tcacgaataa agatggtaat
     7141 gttaatattg caggtaatga tggtaatcca accaaaatta caggcgtgaa agcaggtgaa
     7201 gctgatactg atgcggtgaa tgtaagtcag ttaaaacaag cagcagcgtc acaaaaccga
     7261 agtgaaaggt tcaggtttag caactgttgg tgcacctgaa acaggtgata atggtcaaac
     7321 aatctatacg gtagatgtag cgaaagcagg tgctcctaca gtaactcgtg gtaatgtggc
     7381 tgttaaagaa ggcgatgaaa acaaagttat gaccgcaggt gatgtggctg atgcaattaa
     7441 taattcagag aaaacctcat ctgtagtagc tggttctaaa gccgtaactg ttaaagccgg
     7501 aaaggaagat gataaaggca atactgaata tacggtagat gttgcaacgg ataaatctat
     7561 cggtcgtgat aatgacggta atatgacagt gaatactgac aacgttacta tcgtgaaaga
     7621 tccgacaaca ggcgaagtta aagcgaatac cacaacatta aataattctc ctgaaggtaa
     7681 agttactgag cctacaggtg acgatgctaa gaaattagtg acggcaggtg atattgctaa
     7741 tgcaatcaat aattcaggct ttaatgtaac agcaggtgat ggtgttgatg gtggtgaaac
     7801 caaaggtaag aaaacacagc taatcaagcc aagtgaaaca gttacctttg atgcgggtaa
     7861 aaatatgaca cttacccaag ctgacggtaa attcacttac acaacgaaag ataatgttgc
     7921 attcaacagc attgatatga gcaatggtaa accagataca acaggctcaa tcactaatct
     7981 gaagagcggc gtgggtggta catttgcaga taaagcagca ccaacagatg cagagcgtaa
     8041 agcgattgcg gataatatta acaacgcaac aggtgatacg ttgaataatg ctgtgaatgt
     8101 gggtgatgta caagcagcaa ccaccaaagt ggaaggtgat aaaggtgtta ctgtaacatc
     8161 taaaactaac gatgacggct caaccactta taccgttgaa gcaaaaacgg atggtacaac
     8221 cattaaggta aacgataagg gcgaaatcac ggcaaacacc agcgagttaa caaataaccc
     8281 tgatggtaaa gtggaagagc ctacagaacc aaatgcatta gtaacagcta aaaccgttgc
     8341 agatgcaatt aacaatgcag gttttaacat tcaagcaaac ggtgacgaga agagcctggt
     8401 gaaaacaggt gatacggttc agttcttaaa tggtaaaaac attgagatta cccgtgatgg
     8461 gaacattact gtttcaactg caaaaaatgt gaattttgat agtgtacagt tcggaaatga
     8521 aggtcctaag attactaaca atggtggtaa tatcaatgtt ggtgacaaag acggtaatgc
     8581 agtcaaagta a
//
LOCUS       DANKBV010000096         8517 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.096, whole genome shotgun sequence.
ACCESSION   DANKBV010000096 DANKBV010000000
VERSION     DANKBV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8517
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.096"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            20..2242
                     /gene="glgB"
                     /locus_tag="PWY43_001789"
     CDS             20..2242
                     /gene="glgB"
                     /locus_tag="PWY43_001789"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HDL2136651.1"
                     /translation="MQDYTYSEKDAFFVERLSSGSCNDPFSYLGIHPSNNGVIIRVFL
                     PSAIACQVIDLNGKPVSSMLKIDDRGFFIAELIGKKTDFSYRLLVEYPDLTVDIEDPY
                     RFKTHIQELDNWLFAEGKQLRPYEKLGAHLVTQNEVGGVHFSVWAPNAQRVSVVGDFN
                     HWNGLVHSMRFHPDSGIWDIFLPNVKKGACYKFEVLDRNGVLRLKSDPYAFASQFRPE
                     TASIVSGLPSIVPAEKTCRNANAPDKPISIYEVHLGSWRRNIENNYWLSYEELADELI
                     PYVKDMGFTHIELLPISEFPFDGSWGYQTTGIYSPTSRFGTPEGLSYFIQKAHDKGIG
                     VILDWVVGHFPVDEHGLVYFDGTHLYEHSDPKEGYHKDWNTLIFNYGRNEVSNYLSGN
                     ALYWIERFGIDALRVDAVASMIYRDYSRSDGEWIPNKYGGRENLEAIDFLRHTNNILR
                     DESQGGASIAEESTSFAGVSHSTERGGLGFNYKWNMGWMNDTLRYMQKDPIYRRYHHN
                     LMTFGMTYQYSEQFVLPLSHDEVVHGKGSLLNKMPGDCWQKFANLRAYYGYMWGYPGK
                     KLLFMGNEFAQGKEWNFAESLDWYLLGEEGGEWHKGVLGWVKALNYLYTQTPALFEQD
                     YNPEGFEWLVMDDHDNSVFAFERKAKNGDSVIVVSNFTPIVRENYRFGVNELAEYQEI
                     LNSDLACYCGSNVSNANRFASELIASHGKAYSLSITLPPLSTIFLKRAADKKTTQNIT
                     "
     gene            2261..4270
                     /gene="glgX"
                     /locus_tag="PWY43_001790"
     CDS             2261..4270
                     /gene="glgX"
                     /locus_tag="PWY43_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDL2136652.1"
                     /translation="MHYFRGKPFPLGNKLDRQAGEFGTNFAIVSQNATAIELCLFDEN
                     HHETRIPMFSSGDTWHIFVAGVQAGTKYGYRVKGIQDEQAGLLFNPQKLLTDPYAKAI
                     EGKPDLSSAEAAAWYKWNDERDNASLAPKSIVIDEHFDWENDSQLFTPWAETIIYEMQ
                     VKGFSQRNFQIPSDIRGTFAGLVHPVSIDYLKTLGITAVELLPVYHIDETHLQKQGLV
                     NYWGYNVLGNFAIDPNLAADKQNPLKEFKQLVKTLHQAHIEVIMDIVFNHTAESDKNG
                     PMLSFRGIDNTAYWLDKQGEYENWTGCGNTLNLSNPYILCWAIDCLIYWVEECHIDGF
                     RFDLASILGRTPSFSEKADFFTALKSHSKLTHIKLIAEPWDIGAEGYQLGQFPEPFYQ
                     WNDCYRDDIRRFWLQNQGDWGLLARRLAGSDDVFAKYSVSKSINFITAHDGFTLQDLT
                     SYQQKHNWANGEQNRDGHNSNYSNNHGVEGQTADELVKKQREITACSLLATLFLSAGV
                     PMLLAGDEIGHSQQGNNNSYCQDNEITWLDWENMNHTRLDYVRSLIRLRKKIALLSKQ
                     NNWWQEDSVQWLNPSAQLMNIDDWHNQHNGLVVLLQEEWLLLFNANRQPQPFLLPQGN
                     WKVVLEQAQGTLMTDTQAVTLNNMGVCVLQKSDVMFANKLETQYA"
     gene            4263..5576
                     /gene="glgC"
                     /locus_tag="PWY43_001791"
     CDS             4263..5576
                     /gene="glgC"
                     /locus_tag="PWY43_001791"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDL2136653.1"
                     /translation="MLNQESTALVARQDLTNDTLVLILAGGRGSRLYELTDKRAKPAV
                     YFGGSRRIIDFALSNCINSNLLKIGVITQYAAHSLLRHLQRGWSFLPYERNQYIDMLP
                     ARQQLDENTWYRGTADAVYQNMAMMKSHYRSKYVVILAGDHIYKMDYTQMLKDHVDQG
                     AKCTVGCIEVPRSQATEFGVMAVNEKLKVKEFVEKPADPPAMPGKPDSSLASMGIYVF
                     NADYLYEMLEKEASTPGTSHDFGKDIIPKAVEQGVLYAHPFERSCKGRNASGAIYWRD
                     VGTIDSYWAAHMDLVSEDPELDLYDESWPIHGRPQQTAPARFFYNRARARTLDNSLIA
                     GGCVITDAEISNSVLFNRVHVEAESVIEHTVILPQVTIGKNCVIKRAVIDRHCVIPDG
                     LEIGVNAEEDAKRFRVSKNGIVLVTQSMLDKLAGKEANSDIKYTI"
     gene            5699..7135
                     /gene="glgA"
                     /locus_tag="PWY43_001792"
     CDS             5699..7135
                     /gene="glgA"
                     /locus_tag="PWY43_001792"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDL2136654.1"
                     /translation="MKVLHICSEMYPLIKTGGLADVMGALPFAQQQAGNDVRVLIPYY
                     PKVAQTLGETVEVATIGTFAGTVSVRFAYFNGLGVYVIDAPHLFMRDGNPYHDSNYQD
                     YPDNYKRFALLGYIGAQLAQGLDHWWGTAQVLHAHDWQAGLACAYLKSWGSNVKTLFT
                     IHNIAYIGRFNAFHLAELGLPWEFFHAEGLEFYGEISYLKAGLYFADRITTVSPTYAR
                     EITEEIAGAGLHGLLATRQYQGRLRGILNGVDAEVWNPETDTHIVVNYRANYMHGKAK
                     NKAELQRIFGLPEDKEALLMVMVTRLTEQKGADFLLTNIDTLMQHKVQIIVLGSGSPD
                     LENALRYFQHKYPNQIGVKIGYDEALSHQIIAGGDLILVPSRFEPCGLTQLYGLKYGT
                     LPLVRRTGGLADTVVDSHKESIENRSATGFVFDYPDAPAFLEAAMRAISLWQKQKLWS
                     SVRQNALAQDFGWAKVAEQYQNLYHEII"
     gene            7230..>8517
                     /locus_tag="PWY43_001793"
     CDS             7230..>8517
                     /locus_tag="PWY43_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDL2136655.1"
                     /translation="MSRQSLSPEMIKNVKNAILHKMVFALGVEPRDASKRNWLNATLR
                     VVRDLSTEDWLQTRRSQVANGSRRVYYLSMEFLMGRTFSNAMISEGVYELIGAALKEL
                     GQDLEEIINEEGDPGLGNGGLGRLAACYMDSLATMKIPAIGYGIQYEYGMFRQEIRNG
                     EQIEQPDEWLENEFVWPYLRSSKRFPVRFGGKTWREGKKVVWQPEEEITAQAHDQLIP
                     GFETTSTNSLCLWSAHASGRGFGLSDFNRGDYFAAMVKQNSSENVSRVLYPDDSTYNG
                     RELRLRQEYFLCSASVQDIIRRHEAEFGSCVNLADKVAIHLNDTHPTLAVPELMRILI
                     DEKGYGWEQAWAMTRKIFFYTNHTLMSEALETWPVEMLARVLPRHLEIIFDINEYFLE
                     EVRSQFPEDHDLISRVSLIDEQGDRRVRMAWLAVVAS"
BASE COUNT         2449 a         1611 c         2003 g         2454 t
ORIGIN      
        1 ctttaagcaa ggagtgccga tgcaagacta tacttattct gaaaaagatg ctttttttgt
       61 tgagcgactt tcttcgggtt cttgtaatga tcctttctcc tacctaggta ttcaccctag
      121 caataacggc gttattatta gggtatttct tccttctgct atagcttgcc aagtgatcga
      181 tttaaacgga aagcctgtgt catcaatgct aaaaattgat gatagaggct tttttattgc
      241 agagctgatt ggtaaaaaaa cagatttttc ttaccgcttg ttggtggaat accccgattt
      301 aacagtagat attgaagacc cttaccgttt taaaacccac attcaagagc tggataactg
      361 gctgtttgcc gagggaaaac aacttcgccc gtacgaaaaa ttgggggcac atttagtcac
      421 gcaaaatgag gttggtggtg tgcattttag cgtatgggca ccaaatgcac agagggtttc
      481 tgtggttggg gatttcaacc actggaacgg tttggtgcat tcaatgcgat tccaccccga
      541 ttccggcatt tgggatattt tcctgccgaa tgtgaaaaaa ggggcttgct ataaatttga
      601 agtgttggat cgaaacggcg tgttgcgttt gaaatctgac ccttacgcct ttgcctccca
      661 attccgcccc gaaaccgcat ctattgtttc cggcttgcca agcattgtgc cagccgagaa
      721 aacctgccgt aatgcgaatg ctcccgataa accgatttcc atttatgaag tgcatttagg
      781 ctcttggcga cgcaatattg aaaacaatta ttggcttagc tatgaagaac tcgctgatga
      841 actcattcct tatgtgaaag atatgggctt tacccatatt gaattgctgc ctatttccga
      901 atttccgttt gatggctctt ggggttatca aaccaccggc atttattcgc caaccagccg
      961 ttttggtacg cctgagggct tgagttactt tatccaaaaa gctcacgata aaggcattgg
     1021 tgtgattctt gactgggtag tggggcattt tccggtagat gaacacggct tggtttattt
     1081 tgatggaacg catttatacg aacattccga tccgaaagag ggctaccaca aagattggaa
     1141 taccttaatt tttaactacg gcagaaatga ggtgagcaat tatttgtctg gtaatgcact
     1201 ttattggatt gagcgttttg ggattgatgc gttgcgggtg gatgcggtgg cgtcaatgat
     1261 ttatcgtgat tactcacgtt cagacggcga gtggattcca aataaatatg gcggacgtga
     1321 gaacttagag gcaatcgact tcttacgcca caccaataat attttgagag acgaaagcca
     1381 aggcggtgcg agcattgcag aggagtccac ctcttttgcc ggcgtgtctc actccactga
     1441 aagaggcggt ttgggcttta attataaatg gaatatgggc tggatgaacg acactttacg
     1501 ctatatgcaa aaagatccaa tttaccgccg ttatcaccac aatttaatga cttttggtat
     1561 gacttaccaa tacagcgaac agtttgtgtt accgctttca cacgatgaag ttgttcacgg
     1621 caaaggttca ttgctgaata aaatgccggg ggattgctgg caaaaatttg cgaacttacg
     1681 agcctattat ggctatatgt ggggctatcc gggcaaaaaa ttgctcttta tgggcaacga
     1741 gtttgctcaa ggtaaagagt ggaattttgc cgagagctta gattggtatt tactcgggga
     1801 agaagggggc gaatggcata agggcgtatt gggttgggta aaagccctta actatttata
     1861 cacccaaacc cctgcactgt ttgagcaaga ttacaatcct gaaggttttg agtggttagt
     1921 gatggacgat cacgataatt cggtgtttgc ttttgagcgt aaagccaaaa atggcgattc
     1981 ggtgattgtg gtgagtaatt tcaccccgat tgtgcgtgaa aattaccgtt ttggggtgaa
     2041 tgaattggca gaatatcagg aaattctcaa ttctgattta gcttgttatt gcggtagtaa
     2101 tgtcagcaat gcaaaccgat ttgccagcga gttaattgca tcgcacggta aagcctactc
     2161 cttgagcatt actttgccac cgctatcgac tatcttcctc aaaagagccg cagacaaaaa
     2221 aacaacacaa aacataactt aaagaaacat aaaaaataat atgcactatt ttagaggtaa
     2281 gccttttcct ttaggcaata aacttgatag acaagcgggt gaatttggca caaattttgc
     2341 cattgtttcg caaaatgcaa ccgctattga gttatgtctt tttgatgaaa atcaccacga
     2401 aacccgtatt ccgatgtttt cttccggaga tacttggcac atttttgtag caggtgtaca
     2461 agcagggaca aaatatggct acagggtgaa aggcatacaa gatgaacaag ccggtttgtt
     2521 atttaatccg caaaaattat tgactgatcc ttacgctaaa gcgattgaag ggaagccgga
     2581 tttaagtagt gcagaggcag ccgcttggta taaatggaac gatgaacggg ataatgcctc
     2641 tcttgcacct aagtctatcg tgattgatga gcattttgat tgggaaaatg atagccagtt
     2701 attcactcct tgggcagaga ctattattta tgaaatgcag gtgaaaggtt ttagtcagcg
     2761 gaactttcag attccaagcg acattcgagg cacttttgcc ggtttggttc accctgtttc
     2821 gatagactat ttaaaaacct taggaatcac cgcagtagaa cttctacccg tttaccatat
     2881 tgatgaaacc catttgcaga aacaagggct tgtgaattat tggggctata atgtattagg
     2941 taattttgct attgatccga atttagcagc agataagcaa aatcctctta aagagtttaa
     3001 acagctcgta aaaacattac atcaagcaca tatcgaagtg attatggata ttgtgtttaa
     3061 ccacacagct gagagtgata aaaacgggcc gatgctaagt tttagaggca ttgacaatac
     3121 agcttattgg ctagataaac agggcgaata cgaaaattgg actggctgtg gtaatacctt
     3181 aaatttgagc aatccttata tactctgctg ggcaatcgat tgcttaatct attgggtgga
     3241 ggagtgccat attgatggat tccgctttga tcttgcctca attctaggta gaacaccgag
     3301 tttttctgaa aaagcagatt ttttcacagc acttaaaagc cattctaaat taacacatat
     3361 taagttgatt gctgagcctt gggatattgg tgcagaaggt tatcaattag ggcaattccc
     3421 tgagcctttt tatcagtgga atgattgtta tcgtgatgat atacgccgtt tttggttaca
     3481 aaaccaaggt gattgggggc ttttggcacg gcgattggca ggaagtgatg atgtgtttgc
     3541 taaatattcc gtttcaaaaa gtatcaattt tattacggca cacgatgggt ttacgttaca
     3601 agatctcacc agctatcagc aaaaacataa ttgggcaaat ggtgaacaaa accgagacgg
     3661 gcataatagt aactacagca ataaccacgg tgttgaaggg cagacagcag atgaattggt
     3721 aaaaaaacag agagaaatca ccgcttgtag tttgcttgct acgctctttt tatcggctgg
     3781 tgttccaatg cttttagccg gtgatgaaat cggacatagt caacaaggta acaacaatag
     3841 ttattgccaa gataatgaga tcacttggct agattgggaa aatatgaatc acacacggct
     3901 ggattatgtt cgtagtttaa tccggttgcg taaaaaaatc gctttgcttt ccaagcagaa
     3961 taattggtgg caagaggatt cggtacaatg gttgaatcct tctgctcagc taatgaatat
     4021 tgacgattgg cataatcaac ataatgggct tgtcgtttta ttacaggaag aatggctgct
     4081 tttattcaat gctaatcgac aacctcaacc gtttttactg ccacaaggca actggaaagt
     4141 tgtgttggag caagctcaag gtactttgat gacagacacg caagcggtta cgctcaataa
     4201 tatgggggta tgcgtacttc aaaaaagtga tgtaatgttc gctaataaat tggagacaca
     4261 atatgcttaa ccaagaatca acggctcttg ttgcaagaca agatttaacc aacgatacct
     4321 tagtattaat tcttgcagga ggacgaggtt ctcgcctata cgaattaacc gataaacgtg
     4381 caaaaccggc agtctatttc ggtggtagcc gccgtattat tgattttgcg ttatcaaatt
     4441 gtattaactc gaatttatta aaaatcgggg tgattaccca atatgcagca cactctttgt
     4501 tgcgccattt acaacgtggt tggtcatttt taccttatga acgtaaccaa tatattgata
     4561 tgttaccggc acgccaacag ctagatgaaa acacttggta tcgtggcaca gcggatgcgg
     4621 tgtatcaaaa tatggcgatg atgaaatcgc actatagatc aaaatatgtg gtgattttag
     4681 caggggatca tatctataaa atggactaca ctcaaatgct gaaagatcac gtcgatcaag
     4741 gtgcaaaatg tactgtaggt tgtatcgaag tgcctcgctc gcaagcaaca gagtttgggg
     4801 taatggcggt gaatgaaaag ctgaaagtga aagagttcgt cgaaaaacca gctgatccac
     4861 ctgcaatgcc gggaaaaccg gattcctcgc tagcatcaat ggggatttat gtatttaatg
     4921 cagattatct atatgaaatg ctggaaaaag aagcctctac accgggaacc tcacatgatt
     4981 tcggtaaaga tattattcca aaagcagtag agcaaggtgt gctatatgcc cacccatttg
     5041 aacgttcttg taaagggcgt aatgccagtg gggcaattta ctggcgagat gtcggtacga
     5101 ttgacagcta ctgggctgcc catatggatt tagtttctga agatccggaa ttagatttat
     5161 atgatgaatc ttggccgatt cacggtcgtc cgcaacaaac tgcaccggca cgttttttct
     5221 ataatcgtgc gcgtgccaga accctagata actcactgat tgcgggtggt tgtgtgatta
     5281 ccgatgcgga aatcagtaat tcggtcttat ttaaccgtgt tcacgttgaa gcggagtcgg
     5341 tgattgaaca cacagtgatt ttaccgcagg tgactatcgg taaaaattgc gtaattaagc
     5401 gcgctgtgat tgacagacat tgtgtaattc ctgatggctt agaaattggt gtaaatgccg
     5461 aagaagatgc gaaacgtttt cgtgtcagca aaaatggcat tgtattagtg acacagtcaa
     5521 tgttagataa attagcggga aaagaagcca attcggatat taaatataca atttagtaag
     5581 agatgatata atgcttcgag tttgtcattt gaggtcgcaa gctcgaagta ttgttatgat
     5641 taaagcgctt caagcggtct tatttttcaa ctattttaca catagaaaac ggttaaaaat
     5701 gaaagtttta catatttgct ctgaaatgta ccctttaatt aaaacaggcg gtttagctga
     5761 tgtaatgggg gcattgccat ttgcgcaaca gcaagctggt aatgatgtca gagtgttaat
     5821 tccttattac ccgaaagtcg cgcaaacttt aggggaaacg gtagaagttg ctactatcgg
     5881 cacttttgcc ggaacagtct ctgttcgatt tgcttatttt aatggcttag gcgtttacgt
     5941 tatcgatgcg cctcatttat ttatgcgtga tggtaaccct tatcacgaca gcaattatca
     6001 agactatccg gataactata aacgctttgc tttattaggt tacattgggg cgcaattagc
     6061 ccaaggtttg gatcattggt ggggaaccgc tcaagtttta cacgcacacg attggcaagc
     6121 agggcttgct tgtgcctatc ttaaaagttg gggaagcaat gtaaaaacgc tcttcactat
     6181 tcataatatt gcctatatcg ggcgctttaa tgcctttcat ttagcagagc ttggcttacc
     6241 ttgggaattt ttccacgccg aaggcttaga attttacggt gaaatttctt acctcaaagc
     6301 aggtttgtat tttgctgatc gcattactac cgttagcccg acctatgctc gtgaaattac
     6361 tgaggaaatt gccggtgcag gcttgcatgg tttactggca acccgtcaat atcaaggtag
     6421 attgcgtggc attttaaacg gggtcgatgc tgaggtgtgg aatccggaaa cggatacaca
     6481 tattgtggtg aattatcgtg cgaactatat gcacggtaaa gccaaaaata aagcggaact
     6541 ccaacgaatt tttggtttgc cagaagacaa agaagcatta ttgatggtaa tggtgactcg
     6601 tttaactgag cagaaggggg cagatttctt actcaccaat atagatacct taatgcaaca
     6661 taaggtacag attattgtat tgggaagtgg atcgccagat ttagaaaatg cgctacgtta
     6721 tttccaacac aaatacccga accaaattgg ggtgaaaatc ggttatgacg aggctttatc
     6781 gcaccaaatt attgcaggtg gtgatctgat tctagtacca agccgttttg agccgtgcgg
     6841 attaactcaa ctttatgggc tgaaatatgg cactttaccg ctggttcgcc gtaccggtgg
     6901 cttagctgat acggtggtag atagccataa agagagcatt gaaaaccgca gtgccaccgg
     6961 atttgtattt gattacccag atgcacctgc gtttttagag gcagctatgc gggcaatcag
     7021 cttatggcag aaacaaaaat tgtggtcgag cgttcgccag aatgcccttg ctcaagattt
     7081 tggttgggca aaagtggcag aacagtacca aaatttatac cacgaaatta tttaatttgt
     7141 attcacaaca tcatttcaag cggtcgaatt tgcggatttt tttgtaaaaa cgaccgcttg
     7201 caatattaaa aattaggaga atttactcaa tgagcaggca gtcattaagc ccagaaatga
     7261 tcaaaaatgt taaaaatgcg attttgcaca aaatggtgtt cgctttaggg gtcgaacccc
     7321 gtgatgccag taagcgtaac tggctcaatg caaccttacg tgtagtgcgt gatttatcta
     7381 ccgaagattg gttacaaacc cgccgtagcc aggttgcaaa tggtagccgc cgtgtttatt
     7441 acctttcaat ggaattttta atggggcgta cgttcagtaa cgcaatgatt tctgaaggtg
     7501 tgtatgagct tattggcgct gcgttaaaag agttagggca agatctggaa gagattatca
     7561 atgaagaggg cgatccgggc ttaggtaacg gtggcttagg gcgtttagcc gcctgctata
     7621 tggacagcct tgccacaatg aaaattccgg caatcggcta cggtattcaa tatgaatacg
     7681 gtatgttccg ccaagaaatc cgcaacgggg agcaaattga gcaacctgat gaatggttag
     7741 agaatgaatt tgtgtggcca tatctacgtt caagtaagcg tttcccggta cgctttggcg
     7801 gtaaaacttg gcgtgagggc aaaaaagtag tatggcagcc ggaagaagaa attaccgctc
     7861 aagctcacga ccaactgatc ccgggctttg agaccacctc aactaacagc ttatgtttat
     7921 ggtctgcaca cgcatcgggt agagggtttg gcttatctga ttttaaccgt ggcgattatt
     7981 ttgcagcaat ggttaaacaa aacagctcgg aaaatgtctc tcgtgtgctt tatccggacg
     8041 attctaccta taacggtcgt gaattacgct tacgccaaga gtatttcctt tgctcggcat
     8101 ctgtacaaga tattatccgc cgccacgaag cggagttcgg ctcttgtgtg aatttagccg
     8161 acaaagtggc aattcactta aatgacacac acccaaccct tgcggtgcct gagttaatgc
     8221 gtattttaat tgacgaaaaa ggctatggct gggaacaagc gtgggcgatg actcgtaaaa
     8281 ttttcttcta caccaatcac actttgatga gtgaagcgtt agaaacttgg ccggtagaaa
     8341 tgttagcccg tgtgctacca cgccatttag agattatttt cgacattaac gaatatttct
     8401 tagaggaagt gcgtagtcaa ttcccggaag atcacgattt gatttctcgt gtttccttaa
     8461 ttgatgaaca aggcgatcgc cgtgtgcgta tggcgtggct tgcggtagtc gcctcag
//
LOCUS       DANKBV010000097         8507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.097, whole genome shotgun sequence.
ACCESSION   DANKBV010000097 DANKBV010000000
VERSION     DANKBV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.097"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            501..2888
                     /locus_tag="PWY43_001794"
     CDS             501..2888
                     /locus_tag="PWY43_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDL2136656.1"
                     /translation="MEKMMRKINQLIISPYFFLSILSASANSDFWKSDLNFNLTNITK
                     RVGVDNFTTPVAGEPWAGIGPNGEAAGTAPLYYSRIPAMQVTEDNKLVVMFDLRWNRA
                     YDQDRIDPGIAISSDGGHTWTKKTAWSFDRTNHPARRSMDPTLLHNPIDNSLYVMHGT
                     WSMSDQQWYGGRVPHFNSGSWAATIYKSTDGGLNWEKNTEFSKFSNLDVFSKVTKNGK
                     PTLGFLGGVGSGIVMQDGTLVFPIQTAHQNGIATTIMYSKDNGKTWEMPEIDNPVAPN
                     QSSLENMVFELEPGKLVMTGRGNSRWAYSSTDMGKTWELFTPTNGLLPTSSQPTQGSS
                     IYVTLPNGRKVLLISKPDGKNDGWKRGDITLWMLDAKDPSHKHKVHIIRPGSGNAAGA
                     GYSSLAYKEGNLFVAFENDGDISVKNLTEHIAEMEAKAIEWGLPDELTPALDKINALP
                     YLNKAQKETLVEKMRRANDTAIAQSFVINKEMYNLKNNSDELDKLAKNITKALPSQQN
                     IYQRALAYVNKVTNTADTTYLNYNGIMDTYANLYESYLALNTKLDFTRYIQKARKFNE
                     YNTDILYRSFDNFFAHYDTGVKHNNLSLGLNTKLSENLRGGLFFEYSNKNRNSVQIGA
                     RAKYEMDNHQLSGFLRYRGVKHKDMLARNNSVDGYLNYAYRIQLDDKLSISPSVGAYV
                     SRSSRSLIDEDLAINSRTVYASDVGLNIHYKLNDIDAYIRPSIGFVNGDITLSQSNDM
                     TNTYKIKDKSNVYSVTTGLKKRFANGVALGADFKLHRYGSQTTESNFGLNVSYNW"
     gene            complement(3055..3591)
                     /gene="hpt"
                     /locus_tag="PWY43_001795"
     CDS             complement(3055..3591)
                     /gene="hpt"
                     /locus_tag="PWY43_001795"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL2136657.1"
                     /translation="MEKHHIETLHSKEEVLNRINELAKEINQHYHQKQCQNLVVVGLL
                     RGSFMFMADLVRKLELPVEVDFLTASSYGSSTESSRDVKILKDLDGEIQGKDVLIVED
                     IIDTGFTLSKVGEILKLREPNSLAICTLLDKPCRREVDVPVKWVGFEIPDEFVVGYGI
                     DYAQKYRHLDYIAKVVIE"
     gene            complement(3630..4979)
                     /gene="pmbA"
                     /locus_tag="PWY43_001796"
     CDS             complement(3630..4979)
                     /gene="pmbA"
                     /locus_tag="PWY43_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDL2136658.1"
                     /translation="MSITDKQTLLQQEQELRQAVEFALNFAKQAGAEAEVGVTKVAGL
                     SVSSRLEQVENIEFNNDGSLGISVYLVKRKGNASTSDLQPESIQKTVEAALAIAKYTS
                     EDACTGLADKELLAFEAEDLELYHQADISVDQAVELAIEAEHYALNADEKIVNSDGAT
                     FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTISREFDKLQAPK
                     WVGEQAAIKAVDRLNPQRIKTGEMPVIFYNDVATGLVGHLAGAISGGALYRKSSFLLD
                     KLGEQILPSWFAISERPHLLRQLASSAFDSEGVITQDREIITDGILQTYLMTSYSGRK
                     MGLKSTGHAGGIHNWLVKPNRTGGLNSLLKEMGTGLLVTEMLGSTINSVTGEYSRGAA
                     GFWVENGEIQYPVAEITIAGQLQDMYKNLVAVGDDIEHRSNIQTGSILLEKMMVSGE"
     gene            5078..5608
                     /locus_tag="PWY43_001797"
     CDS             5078..5608
                     /locus_tag="PWY43_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDL2136659.1"
                     /translation="MAKKRSKNEIDWTDEEEEIIWVSKNEIKRDSEHLKKIGAELIEL
                     TPQNLEKIPLDDDLKEAIRQAQGFKMEARRRQIQFIGKLLRNRDQEPIQEALDKVKNR
                     HNQQQALLHKLELVRNQLIAMGDASLDNLLTEYPQLDRQHLRNLIRGAIKEREANKPA
                     KNYREIYQYLKTEIVE"
     gene            5614..5985
                     /locus_tag="PWY43_001798"
     CDS             5614..5985
                     /locus_tag="PWY43_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HDL2136660.1"
                     /translation="MEYLSTILKAHIGLAYISLILLLTRGFLSSKMVDWRQYKILRIA
                     PHAVDTFLLITGIAAVAIFLAYDFFTLAQFSWLLPKLLFLVLYIVFATKAFKKGQPFS
                     LKFYLLAVVSFLLMMLTATFH"
     gene            5998..6711
                     /locus_tag="PWY43_001799"
     CDS             5998..6711
                     /locus_tag="PWY43_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL2136661.1"
                     /translation="MIGPFVNAGAIILGGVLGACLGSHLPERIRTTLSLVFGLCCFCL
                     GMMLTASVKYMSAVALALILGTIIGELIYLENRIGKVADLARGLIDRLFPPVHSLSQE
                     EFLEKFIALLVLFCVSGTGIFGAIKEGMTGDPSILYIKSILDFFTAMIFAASLGYAIA
                     AIAVPLILVQGILILLATEIMPLTTANMMADFSATGGLLLFATGFRICGIKNFPVANM
                     LPALLLAMPMSYFWEWLVH"
     gene            6714..7277
                     /locus_tag="PWY43_001800"
     CDS             6714..7277
                     /locus_tag="PWY43_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL2136662.1"
                     /translation="MKKRIIVGISGASGFQYGYKVLELLKPFSEIETHLVMTKGAELT
                     RTLETDYTREALHELADEIHSLNNVGASIASGSFKTAGMIVAPCSMRTLAAIANGFSD
                     NLLTRAADVVLKERRKLVLMVREAPLNLAHIDNMRKVTEMGGIIFPPIPAFYQQPKNL
                     DEMVTHSVSHALSLLDIDIPNTPHWGE"
     gene            7340..7780
                     /locus_tag="PWY43_001801"
     CDS             7340..7780
                     /locus_tag="PWY43_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDL2136663.1"
                     /translation="MHNPIPTHILDFIQTNHVVNIAAHTEQDFWAANCFYAFDQENAR
                     LIILTAKKTRHAGLMLENPHIVGTICGQIEKINEIEGVQFSATAECLEDKQAEKAALQ
                     IYYQKHPLARLKPSDVWVLSFNTIKHTGNKLMFAKKTIWERNII"
     gene            complement(7784..>8507)
                     /locus_tag="PWY43_001802"
     CDS             complement(7784..>8507)
                     /locus_tag="PWY43_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2136664.1"
                     /translation="LVILLSGRSEIDFNSEVFELDASLKPQAAFLPLNRAEMGRKIFR
                     KGQLQKELVIFLYPKWLIESQLNLASYHHFKQHLKAHQIILTQSMLKLIHDIVSEQEM
                     QPFGLLEKEGNLLALLASAFSQLTQAPRLKKDRVQQLTELLESGKFDGWSLKQIAIYF
                     HTNVTTLQNEFQHYHKTSIFAYLRRSKLEQAYKLLLQGQSITQAAEFAGYANSENFST
                     AFKRTFGITPSQIKRDKAKGII"
BASE COUNT         2557 a         1687 c         1616 g         2647 t
ORIGIN      
        1 ttcaagtctc gaccaggcac catttgacta aatttagttc aacagagcta aacgaaaaaa
       61 ccttagaaag cctgtaaaat caacgtttac aggctttttt gttgttttag ctcaatggca
      121 ctaaacattt catatcgcaa attttaagta gcctagcaaa ttttccgcca aatatgaccg
      181 cttgtaatgc cgttaggtta ttgcctggcg gcctttttgt tacagctctt atatttttta
      241 caccaaagtt cagaaaaagt cacttttatc actaacatta agatgaaacc ttgatacaaa
      301 gcttgggctt cggtgatatt taaaaaatat cacttttttc aataattgtg acaaatctct
      361 cagatttttt tatttttttg tgcttaaatc ttaatcataa aatgccgtta taattacttg
      421 ctatctggta ttttatacat aagattgaac catataatag ctgttcagct aaaaattagt
      481 ttttgcactt tatcccccta atggagaaaa tgatgagaaa aatcaatcag ttaatcatat
      541 ccccatactt tttcctaagt attttatctg cttccgctaa ctctgatttt tggaaaagcg
      601 accttaattt taatctcaca aatattacca agcgtgtcgg agtcgataac tttaccactc
      661 cagtagctgg cgagccttgg gctggtattg gtccaaatgg tgaagctgca ggtactgcgc
      721 cattatatta tagccgtatt ccagctatgc aagttaccga agataacaaa ttagttgtta
      781 tgtttgattt acgctggaat cgtgcgtatg accaagatcg tattgatcct ggtattgcga
      841 tttcttccga tggtggccat acttggacga agaaaaccgc ttggtctttt gatagaacta
      901 atcacccagc acgccgttca atggatccaa cgctattaca taatcctatt gataatagtt
      961 tatatgtaat gcacggaact tggtctatgt cggatcaaca atggtatggt gggcgtgttc
     1021 ctcactttaa tagtggcagc tgggcagcta ccatttataa atctactgat ggtggtttaa
     1081 attgggagaa aaatactgag tttagtaaat tcagcaattt agacgtattt tctaaagtca
     1141 ccaaaaatgg aaaacctacc ttaggctttt taggcggggt cggatctgga attgtaatgc
     1201 aagatggtac gttggttttc ccaattcaaa ccgcacatca aaatggtatt gcgaccacta
     1261 ttatgtattc taaggataat gggaaaactt gggaaatgcc agaaattgac aacccagttg
     1321 caccgaacca aagttcttta gaaaatatgg tgtttgaact cgaaccgggt aagctagtga
     1381 tgactggacg aggaaacagt cgctgggcat actcttctac tgatatgggt aagacctggg
     1441 aattatttac tccaactaat ggattattac caactagctc acaaccaact caaggctctt
     1501 ctatctatgt aacactacca aatggtcgaa aagtgctact aatctctaaa ccagatggaa
     1561 agaatgatgg gtggaaaagg ggtgatatta cactttggat gctagatgca aaagatccat
     1621 cgcacaaaca caaggtgcat attattcgtc caggttcagg taatgccgct ggtgctggtt
     1681 attcctcttt agcctataaa gaagggaatt tatttgttgc ttttgaaaac gatggtgata
     1741 tttcagtaaa aaatttaact gaacatattg ctgaaatgga agcaaaagct atcgaatggg
     1801 gattaccaga tgaacttact cctgctttgg ataaaatcaa tgcactgcct tatctcaata
     1861 aagctcaaaa agagacgcta gtcgaaaaaa tgcgtcgtgc taatgatact gcgattgcac
     1921 aatcttttgt gattaacaaa gaaatgtata atctgaaaaa caatagtgat gagctggata
     1981 aattggcaaa aaatattact aaagcactgc cgtctcaaca gaatatttat caaagagcct
     2041 tagcctatgt gaataaagtc accaatacag cggataccac ttatttaaat tataatggta
     2101 ttatggatac ctatgctaat ttatatgaaa gctatttggc tttaaatact aaattagact
     2161 tcactcgcta catccaaaaa gcgagaaagt tcaatgaata taacactgat attttatatc
     2221 gtagctttga taatttcttt gcccattacg acacaggtgt aaaacacaat aatctatcct
     2281 taggcttgaa tacaaaatta agtgagaatt tacgtggtgg cttattcttt gaatacagta
     2341 ataaaaatcg taatagcgta caaattggtg caagagctaa atatgaaatg gataatcacc
     2401 aactttctgg cttcttacgc tatcgtggcg taaaacataa agatatgcta gcacgtaata
     2461 acagtgttga tggctaccta aattatgctt atcgtattca attagatgat aaattaagca
     2521 ttagcccatc agttggtgct tacgtttcac gttcttctcg ctcattaata gatgaagatc
     2581 ttgcgattaa tagccgcacg gtctatgcaa gtgatgtagg attgaatatc cattataagc
     2641 taaatgatat tgatgcttat attcgtccaa gtataggctt tgtcaatggt gatataactt
     2701 tatctcaatc taatgatatg acaaacacat acaaaataaa ggataaaagt aacgtgtatt
     2761 ctgtcacgac agggttgaaa aaacgctttg caaatggtgt ggcattaggg gctgatttca
     2821 aattacatcg ttatggttct caaactactg aaagtaattt cggtctaaat gtaagttata
     2881 actggtaata ccgtaaactg attacgattt aaaagttaga ctagcccaag actgagttct
     2941 atattccata gagctcagtc ttttaatttt tctttctcga taagaatatt ttatatttca
     3001 accttaatcg agaatgtaat tagtcgtgaa aatctaagta gtgattgagt ttttttattc
     3061 aatcactact ttagcaatat aatctaaatg acgatatttt tgggcataat caatcccata
     3121 acccaccaca aattcatcgg ggatctcaaa tcccacccat tttacgggca catcaacttc
     3181 gcggcggcaa ggtttatcta ataaagtaca aatagcaagt gaatttggct cacgtaactt
     3241 gagaatctcc cctaccttgc ttaaggtgaa gccagtatca ataatatctt ctacaatcaa
     3301 tacatcttta ccttgaattt cgccatctaa atcttttaga attttgacat cacggctgga
     3361 ttccgtgctg ctgccatagc ttgatgcggt tagaaaatcc acttctacag gtaactctag
     3421 ctttcttact aaatctgcca taaacataaa cgagcctcgc aataaaccaa ccactacaag
     3481 gttttggcac tgtttttggt gatagtgttg attgatttct tttgctaatt cattgattcg
     3541 gtttaatact tcttcttttg aatgtagcgt ttcgatatga tgtttttcca ttgatttctc
     3601 caaataaaat tgattacaag cggccgtttt tactcgcctg aaaccatcat tttttctaat
     3661 aagattgaac ctgtttggat atttgagcgg tgttcaatat catcgcctac cgccactaaa
     3721 tttttataca tatcttgcag ttgtcctgca atggtaattt ctgcaaccgg gtattgaatt
     3781 tcgccatttt caacccagaa acctgccgct ccacgagaat attcgccagt cacgctatta
     3841 attgtcgaac ctaacatttc ggtcactaac aagcccgttc ccatttcctt gagtaagctg
     3901 tttaacccac cggtgcggtt tggctttacc agccagttat ggataccgcc ggcgtgtcct
     3961 gtgcttttca gtcccatttt tctacctgaa tagctggtca ttaggtaagt ttgtaagatg
     4021 ccatcggtaa taatttctcg gtcttgggta attaccccct cgctatcaaa ggctgatgaa
     4081 gcaagttggc gtaataaatg tgggcgttct gaaatcgcaa accagcttgg caaaatttgc
     4141 tcgccaagtt tatctaacaa aaagctggat ttacggtata acgctccacc actaatagct
     4201 ccggccaaat gccccactaa accggttgcc acatcgttat agaaaatcac aggcatttcg
     4261 ccggtcttaa ttcgttgcgg attcaatcta tctaccgctt taattgcagc ttgctcccct
     4321 acccatttcg gtgcttgtaa tttatcaaac tcacgggaaa tggtgtattc ataatcacgc
     4381 tcaagctgat cttcataagc tgaaatcacg ctacaagaga gtgaatatcg gctcgacagg
     4441 taactctgca acattccgtg cgtattgcca taaacacgca cgccactgtg agaattgaaa
     4501 gttgccccgt cactattcac aattttttcg tctgcattta aggcataatg ctccgcttca
     4561 atcgccaatt cgactgcttg atcaactgaa atatccgctt ggtggtacag ttccaaatct
     4621 tctgcctcaa aggcaagcaa ttctttatca gcaagcccgg tacaagcatc ttctgaggta
     4681 tattttgcaa tcgcaagggc tgcttcaacc gttttttgaa tcgactctgg ctgaagatcg
     4741 gaggtggaag cattaccttt gcgtttgact aaataaaccg aaataccgag agaaccatcg
     4801 ttattgaact cgatgttttc aacttgctct aaacggcttg aaaccgaaag cccggcaact
     4861 ttagtcacgc ctacttccgc ctctgcccca gcctgttttg caaaatttaa ggcaaattcg
     4921 accgcttgtc gtagctcctg ttcttgttgt aacaaggttt gtttatctgt aattgacatt
     4981 ttgttccatt gattgattgc attgatgaat atattctaac tcaaatttct gttagaatac
     5041 ggcatttttt agagtttatg taatgaaagg ataaagaatg gctaaaaaac ggagcaaaaa
     5101 cgaaattgac tggacagatg aggaagaaga aatcatctgg gtcagcaaaa atgagataaa
     5161 acgagattct gaacacttaa aaaaaattgg ggctgaattg atcgaattga ccccacaaaa
     5221 tttggaaaaa attccactag acgatgattt aaaagaggca attcgccaag ctcaaggctt
     5281 taaaatggag gctcgccgcc gtcaaattca atttatcggc aaattattac gcaaccgtga
     5341 ccaggagccg atccaagaag ccttagataa agtgaaaaat cgccataatc agcaacaagc
     5401 cctattacat aaattggaat tagtgcgtaa tcaactgatt gcaatgggcg atgcatcatt
     5461 agataattta ttgacggaat atcctcaatt agaccgccaa catttgcgta atttaattcg
     5521 tggggcaatt aaagagcgtg aagccaataa accggcaaaa aattaccgtg aaatttatca
     5581 atatttaaaa actgaaatcg tggagtgatt attatggagt acctatcaac cattcttaaa
     5641 gctcatatcg gcttagctta tattagttta attttattat taacccgtgg atttttatcg
     5701 tcaaagatgg tggattggcg acaatataaa attttgcgca ttgccccaca cgcagttgat
     5761 accttcttac ttatcaccgg tattgctgct gtcgctattt tcttagctta tgattttttc
     5821 accttagcac aattcagttg gttgctacca aagttactct tcttagtact ttatattgta
     5881 tttgcgacaa aagcgttcaa aaaaggacaa ccgttttcgc tcaaatttta tctgttagcc
     5941 gtagtctcat tcttattaat gatgctaact gccacttttc actaaataag ggaaactatg
     6001 atcgggcctt ttgtgaatgc cggtgcaatt attttagggg gcgtgctggg tgcttgttta
     6061 gggagtcacc tgcctgaacg tattcgcact acgttgtcgc tggtattcgg cttatgctgt
     6121 ttctgcttag gtatgatgct gactgcctca gtaaaatata tgtctgcggt tgccttagca
     6181 ttgattttag gcacgatcat cggtgaactg atttatttag aaaacagaat cggtaaagtt
     6241 gccgatttag ctcgtggctt aattgaccgc ctattcccac cggtgcatag tctttcccaa
     6301 gaagagtttt tagagaaatt tattgctctg cttgtgctgt tttgtgtgag cggtaccggc
     6361 atttttggtg caataaaaga gggaatgacg ggtgatccga gcattcttta cattaaatcg
     6421 attttagact tcttcactgc aatgattttc gccgcttcat taggttatgc cattgcagcg
     6481 atcgcagtgc cgttaattct tgtgcaaggt atcttaattc tactagcaac ggaaataatg
     6541 cctttaacca ctgccaatat gatggcagat ttctctgcaa cgggcggctt attgttattt
     6601 gcaaccggtt ttcgcatttg tgggattaaa aacttcccag tggcgaatat gctaccggca
     6661 ctgctccttg cgatgccaat gtcttatttc tgggaatggt tggtacatta atgatgaaaa
     6721 aacggattat tgtcggcatt agtggggcaa gcggttttca atatggctat aaagtgttgg
     6781 aactgctgaa accgttttca gaaattgaaa cccaccttgt aatgaccaaa ggggcggaac
     6841 tgactcgcac gcttgaaaca gattataccc gagaagctct acacgagtta gctgatgaaa
     6901 tacattcgct gaacaatgtt ggtgcaagca ttgccagcgg ttcatttaaa accgcaggaa
     6961 tgattgttgc cccttgctct atgcgaacgc ttgccgctat tgctaatgga tttagcgaca
     7021 atttactaac ccgtgcggca gacgtggtgt tgaaagaacg gcgtaaactg gtattaatgg
     7081 tgagagaagc tccgctcaac cttgctcaca ttgacaatat gcgaaaagtg accgaaatgg
     7141 gcggaattat ttttccaccg attccggcat tttatcaaca acctaagaac cttgatgaaa
     7201 tggtcaccca cagcgtgagc cacgcccttt ccctgttgga tattgacatt ccaaataccc
     7261 cacattgggg agaataattc ggcttatctt ttgctttttg aaatgctaga atacctgata
     7321 aaaactatca ggtattttta tgcacaatcc aatcccgacc catattttag atttcattca
     7381 aactaaccac gttgtcaata ttgcagctca taccgaacag gatttttggg cagcaaattg
     7441 cttttatgcc tttgaccaag aaaatgctcg cttgattatc ttaaccgcca agaaaaccag
     7501 acacgctggc ttgatgcttg aaaacccaca tattgtcggc acaatttgtg ggcaaattga
     7561 gaagatcaac gaaattgaag gtgtccaatt ctccgctacc gccgaatgtt tagaagataa
     7621 acaagccgaa aaagccgctt tgcaaattta ttaccaaaaa cacccgcttg cacggcttaa
     7681 gcctagcgat gtttgggtat tatcattcaa taccatcaag cacaccggta ataaacttat
     7741 gtttgcgaaa aagacgattt gggagagaaa tataatttga agctcaaata atgcctttag
     7801 ccttatcccg tttgatttgg cttggtgtta tcccaaaagt tcgcttaaat gccgttgaaa
     7861 aattctccga attggcatat ccggcaaatt cagctgcttg tgtaatactc tgcccttgta
     7921 atagtaattt ataggcttgt tctaatttag aacgccttaa atacgcaaaa atagaggttt
     7981 tgtggtaatg ctgaaattca ttttgtaatg tggtcacatt ggtatggaaa tatattgcaa
     8041 tctgcttcaa actccagccg tcaaatttac cgctctcaag caattctgtt aactgttgca
     8101 cccgatcttt ttttaaccgg ggagcctgtg tcagctgaga aaaagcactt gctaataaag
     8161 ctaatagatt accttctttc tcaagtaacc caaatggttg catttcctgc tctgacacaa
     8221 tatcgtgaat aagttttaac atactctgag tcaaaataat ttggtgggct tttaaatgct
     8281 gtttaaagtg atgatagctt gccaaattca actggctttc tattagccat ttagggtaga
     8341 gaaagataac aagctctttt tgaagttgcc ctttcctaaa aattttacgc cccatttcag
     8401 ctctatttag cggtaaaaat gcggcttgag gttttaaact tgcatctaat tcaaatactt
     8461 ccgaattaaa atcaatttcc gatcgaccac tcaacaatat aaccaat
//
LOCUS       DANKBV010000098         8476 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.098, whole genome shotgun sequence.
ACCESSION   DANKBV010000098 DANKBV010000000
VERSION     DANKBV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.098"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..501)
                     /locus_tag="PWY43_001803"
     CDS             complement(<1..501)
                     /locus_tag="PWY43_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL2136665.1"
                     /translation="MNLSNALKLIKYNELDVLEVNHNKFKAKIALQGAQLLSWQPYNA
                     EQDVLWLSEIEPFRIGSAIRGGIPICYPWFGSIKEPAHGTARIQEWRLVEHTCSDENV
                     GLVFALENKAKMEMMLGETCELYFTHLDPEPAQLALHSYFNIGDIEKVEVQGLPTRCF
                     DKLTDQE"
     gene            650..4846
                     /locus_tag="PWY43_001804"
     CDS             650..4846
                     /locus_tag="PWY43_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL2136666.1"
                     /translation="MKTKTFTRSYLASFVTIVLSLPAVASVVRNDVDYQYFRDFAENK
                     GPFSVGSMNIDIKDNNGQLVGTMLHNLPMVDFSAMVRGGYSTLIAPQYLVSVAHNTGY
                     KNVQFGAAGYNPDSHHYTYKIVDRNDYEKVQGGLHPDYHTPRLNKLVTEVVPAAVTNA
                     GTSIKPYLNEERFPMFLRAGSGTQALRGKESNKTTGIAGAYEYLTGGTTLQLSKSSPD
                     HWLDYSSNLYQVSYGPLSTYALPGDSGSGSYAYDMNEKRWVLVGVLNFYNGMDNQFNR
                     SAIIRKDFHEKKFAEDIAGTINNTVQNAQFNWTAQGKSSSLSQSNNVQKLNVDLKDSS
                     IANQNTSLPQENHGKTINFNGKDATIVLKQDIDQGAGALNLNANLTIRPETDQTWQGA
                     GIIVGKDKKVNWQVKNPQGDRLSKLGEGTLYVNGRGQNLGDISVGDGIVILNQQADHQ
                     GRKQAFNTVGIVSGRPTVVLGSADQVNPDNIYFGFRGGRLDLNGNSIAFKRIQNSDKH
                     ARIVNHNRDHISTLIIQGQDPLTSNDLIWGKWASNSPADIYEYTNPYQNKRKDYFRLK
                     GNSRVYYPTNATSNDHWEFLSSNREQAIQKILDAKNLRQRYDTFNGFIGEDASNKTNG
                     ILNVVFDTKTEVNTEQDKLKNIYTMSGGFNLNGELTLKGGTLLLSGHPTPHAYDIKNK
                     HDVVRENDWQDSHFTAKNITVNKMAQLYIGRNVNEVNSHFTATDKAKLNLGFINRSTP
                     SCYDSEYTGTTHCEVQAVISDNIFANLATTAIKGNVKLQNHSQLNLGKANLTGSVQAD
                     QTTHITLANHSHWLNNGTSQIGHLTMEKGSILSLNDKFATTEIPVRFNKMIIQGNLKG
                     NGRINYTANLAKGESDHLQVDGIAEGNFVLAVRNSTTEANPKSSLNLLSLKNSNQEGN
                     KVSISLENNYVDLGTYRYVLENRNHNYHLFNPLIPNSTSKEMNATSVSSIPKKESVTN
                     VPTLDKKETEQNLTQLQKDFSAHQLENQKAKQSMINAQSELRRLNSQLNVLQKYVNSR
                     RLGYYTQQAVLEQISIIQNKIKQTQTIFNDANATVKLTDQKLEEAKLALGSVNDLVLI
                     KASAPAMQATNQDTSMMNIIQADWISQYANTALSELSAQANSALQISNSLDRQLFKQS
                     DKFNVWSSVEHQKTEHKSDLYRPYKQQTNLTQLGIQMPIDNGLMFGVALSKNHANAEF
                     NEGVNGKSNLLMASLYGKWQSQQGTFISLDGSYGKAKNQLYLFGENHFTRRISSIGAN
                     IGHQFDLAGVQIQPTIGARYYHFSGQDYTLGGAKISSPNTHFMTYQAGLKASKTFSLD
                     DWKVEPSITTHYVDASNKRLAVNVNNNNINQRFGRYLKTEAGIRFEHNQWQFDLHAGF
                     INGNEIKKQHFAGVKLGYSW"
     gene            complement(4901..5995)
                     /gene="mobA"
                     /locus_tag="PWY43_001805"
     CDS             complement(4901..5995)
                     /gene="mobA"
                     /locus_tag="PWY43_001805"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL2136667.1"
                     /translation="MSKTIEERISAVILAGGLARRMNGVEKGLQLFEGKPLISHILAR
                     LSPQVSDIWLNVNRSIEQYQQLYPAFSYYQDSLPDFQGPLSGMLAGFEQIESDYLLFV
                     PCDTPFMPELLLQKLKTALRINNVQIAYAHDGERPHPTFALIHRSVQEDLKAYLGSNQ
                     QQLLAFFQSQKSVAVDFSEQKSAFTNVNTLEDLSRVSQFPVKNLAITGYSGTGKTTLL
                     EKLIPKLTACGIRVGLIKHSHHNVDVDKKGKDSYRLREAGANPTMIVCDERWALMTET
                     KQAVEFSQLIAKFNPQEVDLVFVEGFKHETLPKIQLHRKGIEKPLPELDQWTIATATD
                     YPLERENWLDINNIGGIADFIKNWLENKAS"
     gene            6082..6351
                     /locus_tag="PWY43_001806"
     CDS             6082..6351
                     /locus_tag="PWY43_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HDL2136668.1"
                     /translation="MKCERLEELLELLGEHWRKNPDLHLIDILQQLSVEVGEPDNFKA
                     LSDEVLIYQLKMRNAGKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            6415..7053
                     /locus_tag="PWY43_001807"
     CDS             6415..7053
                     /locus_tag="PWY43_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2136669.1"
                     /translation="MKKLALKTAFVALSALFTFNSTAIAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELSYKIPSQIKEKLPSDSKLVQYHVNFLGRQSEDLTRAWAL
                     AMALGAEDKVKTALFEGAQKDAFKSMDDIRSVFLANGITAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFADSSSTNDFIKRYVDAVVFLSKK"
     gene            7137..8177
                     /locus_tag="PWY43_001808"
     CDS             7137..8177
                     /locus_tag="PWY43_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL2136670.1"
                     /translation="MTKLSILNLVPIREGQTAKQAVDSMVKLAKHAESIGIERYWIAE
                     HHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLPNHSPYIVAEQYGTLETLYPNRV
                     ELGLGRAPGTDMRTATALRRNAQSLPFPDEISELKGYFEGTNPVSAYPAQGLNVPFYI
                     LGSSPESAYLAAERGLPYAFASHFAPRFMDDAVRIYRTHFKPSEVLDKPYVILGVNAI
                     AAETDQEAEQLATTQTQFFLNVVTNAQNYLQPPVESDDKVWENFVKAEIVPHFGPVSF
                     QQNALYNQEKTVVRQMTACSLIGSRESVEQQLKSLKARIEIDEIMAVSYIFDEQKQHQ
                     SYTWLKEIADKV"
     gene            8179..8472
                     /locus_tag="PWY43_001809"
     CDS             8179..8472
                     /locus_tag="PWY43_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL2136671.1"
                     /translation="MSKTMQQKAADAHNMCKLKGDSMLDNSDRQIAFEAVYDSEEIAV
                     QAVEFFTQKAKSVETEPCDIQSEITPIIDGFLMKMWITFSCQAEVILFQMAVR"
BASE COUNT         2816 a         1684 c         1577 g         2399 t
ORIGIN      
        1 ttcttgatcg gttagcttat caaaacagcg agttggcaag ccttgcactt ccactttttc
       61 aatatcacca atattaaaat aagagtgcaa tgccagttgt gctggttctg gatctaaatg
      121 ggtaaaatat aactcacaag tttcgcctaa catcatttcc attttcgctt tattctctaa
      181 ggcaaatacc aaaccgacat tttcatcaga acaagtatgc tcaactaaac gccattcttg
      241 aatgcgagct gtgccgtggg caggctcttt tattgaaccg aaccacggat aacaaattgg
      301 tataccgcca cgaatcgcag agccaattct aaacggttct atctcactta accacagcac
      361 atcttgctct gcattatacg gctgccaact tagcaattgt gctccttgta atgcaatttt
      421 agctttgaat ttattgtgat taacttctag cacgtctaat tcattatatt tgattaattt
      481 taatgcatta ctcaagttca taatttattc ctttgatgac taaaataatt gacagcaaac
      541 actacttagt aattttatat aaaataaggg aagacatctt gccaaactat cagttataag
      601 acagaatagc cagaattttt tattacttat aaggataaac ttctcattta tgaagaccaa
      661 aacatttact cgttcttatc ttgcttcttt tgtaacaatc gtattaagtt tacctgctgt
      721 agcatctgtt gtacgtaatg atgtggacta tcaatacttc cgcgattttg ccgaaaataa
      781 aggaccattt tcagttggtt caatgaatat tgatattaaa gacaacaatg gacaacttgt
      841 aggcacgatg cttcataatt taccaatggt tgattttagt gctatggtaa gaggtggata
      901 ttctacttta attgcaccac aatatttagt tagtgttgca cataatactg gatataaaaa
      961 tgttcaattt ggtgctgcag gttataaccc tgattcacat cactatactt ataaaattgt
     1021 tgaccgcaat gattatgaaa aggttcaagg agggttgcac ccagactatc atactcctcg
     1081 attaaataaa ttagtaacag aagttgtgcc tgccgcagtc accaatgcag gtacatctat
     1141 taaaccctac ttaaatgaag aacgcttccc tatgtttctt cgtgctggtt cagggacaca
     1201 agcgctaaga ggaaaagaaa gtaataaaac aactggaatc gctggtgctt atgaatatct
     1261 tactggcggt accacattac aattatctaa aagctcccct gatcactggt tagattattc
     1321 aagtaacctt tatcaagtaa gctatggacc actttcaacc tatgcactac ctggtgatag
     1381 tggttcaggt tcttacgcct atgatatgaa cgaaaaacga tgggtattag ttggtgtgct
     1441 caatttctat aatggtatgg ataatcaatt caaccgctct gcgattatcc gtaaagattt
     1501 ccacgagaaa aaatttgccg aagatattgc aggaacaatc aataataccg tacaaaatgc
     1561 acaattcaat tggactgctc aaggtaaatc cagctctctt agtcaatcaa ataatgtgca
     1621 aaaactcaac gttgatctaa aagatagtag cattgcaaac caaaacactt ctctgccaca
     1681 agaaaatcac ggtaaaacca ttaattttaa tggtaaagat gcaactattg tactaaaaca
     1741 ggatattgac caaggtgcag gtgcattaaa tctgaacgct aatctcacta ttcgtcctga
     1801 aacagaccaa acttggcaag gtgcaggtat tatcgtcggt aaagataaaa aagtgaattg
     1861 gcaagtaaaa aatccacaag gcgatcgttt atctaaactc ggggaaggaa cactctatgt
     1921 aaatggacgt ggacagaatc ttggcgatat cagtgtaggt gatggtattg taatacttaa
     1981 ccagcaagcc gatcaccaag gaagaaaaca ggcctttaat acagtaggaa tcgtaagtgg
     2041 tcgccccact gttgtgctag gtagtgcaga tcaagttaat cccgataata tttactttgg
     2101 atttcgcgga ggtcgtttag acctaaacgg taacagcatc gcctttaaac gtattcaaaa
     2161 cagcgataaa catgctcgta ttgtaaacca caatcgcgat cacatttcta ccttaataat
     2221 acaaggccaa gatcctctca ctagtaatga tcttatatgg ggaaaatggg caagtaatag
     2281 cccagcagac atttacgaat ataccaatcc ttatcaaaat aaacgcaaag attacttccg
     2341 tctgaaaggt aattcgagag tatattatcc aacgaatgct acaagtaacg atcactggga
     2401 atttctttcc agtaaccgcg agcaagcaat acagaaaatc ctagatgcca aaaacttaag
     2461 acagcgctat gacacgttta atggttttat aggggaagat gcttccaata aaactaatgg
     2521 gatattaaat gtcgtgtttg atacaaaaac agaagtaaat acagaacaag ataaattaaa
     2581 gaatatctac acaatgtcgg gaggatttaa ccttaatggt gaactcaccc ttaaaggtgg
     2641 tacattgttg ctttctggtc acccaacgcc acacgcttat gatattaaga ataagcatga
     2701 tgttgtgcgt gaaaacgatt ggcaagacag ccattttact gctaaaaata tcacggtaaa
     2761 taaaatggca caactctata tcgggagaaa tgtcaatgaa gtaaatagtc actttactgc
     2821 gactgataaa gccaaactca atttaggatt tattaatcgt tcaacgccaa gttgctatga
     2881 ttctgaatac acaggcacta cacattgtga agtgcaagcg gtcatttccg ataatatttt
     2941 tgcaaatcta gcaacaaccg ccattaaagg taatgttaaa ttacaaaacc atagccaatt
     3001 aaatttaggc aaagcaaacc tcactggttc tgtacaagct gatcaaacaa ctcatatcac
     3061 tttagcaaat cacagtcact ggttaaacaa tggtacgagc cagattgggc atcttacaat
     3121 ggaaaaaggg tcgatcctta gcctaaacga taaatttgct accacggaaa tcccagtccg
     3181 attcaacaag atgatcatcc aaggtaatct aaaaggtaat ggacgaatta actataccgc
     3241 aaatttagcc aagggcgaat ctgatcatct ccaagttgac ggtattgctg aaggaaattt
     3301 tgtccttgcc gttagaaata gcacaactga agcaaatcca aaaagctcat taaacctact
     3361 aagcttaaaa aatagcaacc aagaaggcaa taaagtttct atttctctag aaaataatta
     3421 tgttgatcta ggtacttatc gttatgtatt agaaaatcgt aatcacaatt accatttatt
     3481 taatccatta ataccaaatt caacctctaa agagatgaat gctacatctg tatcctctat
     3541 tccaaaaaag gaatctgtta ctaatgttcc tactttagat aagaaagaaa ctgaacaaaa
     3601 tcttactcaa ctacaaaaag atttttcagc acaccaatta gaaaatcaaa aagcaaaaca
     3661 atctatgata aatgctcaat ctgagctaag acgactcaat tcacaactga atgtattgca
     3721 aaaatatgtg aattctcgtc gcttaggtta ctatactcag caggcagttt tagaacaaat
     3781 tagcattatt caaaataaaa ttaaacaaac acaaacaata tttaatgacg ctaatgcaac
     3841 tgtaaaactc acagatcaaa agctagaaga agccaaatta gctctaggct ctgtaaacga
     3901 tcttgtatta ataaaagcct ctgctccagc aatgcaagca actaatcaag atacgagtat
     3961 gatgaatatt attcaagcag attggataag ccaatacgct aacacagcac tttctgaact
     4021 ctcggcacag gctaattctg ctctgcaaat cagtaatagc ttagatcgcc aactcttcaa
     4081 acaaagcgat aaattcaacg tatggagcag cgtcgaacat cagaaaaccg agcataaatc
     4141 agatttatac cgcccgtata aacaacaaac caacctgacc caactgggca tacaaatgcc
     4201 gatagataac ggtttaatgt ttggagttgc attatctaaa aaccacgcta acgcggaatt
     4261 taacgagggt gtaaacggta aatcgaatct actaatggca agcctatatg gtaagtggca
     4321 atctcaacaa ggcactttta tcagccttga tggcagctac ggtaaagcaa aaaaccaact
     4381 ctacctattt ggtgaaaacc actttacccg ccgaatttcc tctattggtg ctaacattgg
     4441 acatcaattt gacctcgcag gagttcaaat tcagccaaca ataggagcaa gatactacca
     4501 tttcagcggc caagactata cactaggagg agcgaaaatc agctcaccaa atacccactt
     4561 tatgacatat caagcgggtc taaaagctag taaaaccttt tcattggatg actggaaagt
     4621 tgaaccaagc attacaaccc actatgtgga tgcaagtaac aaacgcttag cagtaaacgt
     4681 caataataac aatatcaacc aacgtttcgg tcgctatctg aaaaccgaag ctggtatccg
     4741 atttgaacat aaccaatggc aattcgactt acacgctggt ttcattaacg gcaacgaaat
     4801 caaaaaacaa cattttgctg gtgttaagct aggatatagc tggtaagaaa aagcccctag
     4861 aactatttct aggggctttt tctactctta aaaagagtat ctaacttgcc ttattttcta
     4921 accaattctt aataaaatct gctattcccc caatattatt aatatccaac caattttctc
     4981 tctcaagcgg gtaatccgtt gcggtggcaa tcgtccattg gtctagctct ggcaatggtt
     5041 tttcaatccc tttgcggtgg agctgaattt ttggcaaggt ttcgtgttta aagccttcta
     5101 cgaaaacgag atcaacctct tgcgggttga attttgcaat taattgagaa aattcgaccg
     5161 cttgtttggt ttctgtcatt aatgcccaac gttcatcgca cacaatcatt gtcgggtttg
     5221 ctcctgcttc acgtaggcgg tagctgtctt tgcctttttt atctacatca acgttatggt
     5281 gggaatgttt aatcagccca acccgaatac cgcaagcggt caattttggg attaattttt
     5341 ccaataaggt ggttttacct gtgccgctgt agcctgtaat tgcaaggttt tttacaggga
     5401 attggcttac tcggcttaaa tcttccaatg tattcacgtt ggtgaatgcc gatttttgtt
     5461 cggaaaaatc aactgccacg cttttttgtg attggaagaa cgcaagcaat tgctgttggt
     5521 ttgagcctaa ataggctttc aaatcttctt gtaccgaacg atgaatcaac gcaaaagtgg
     5581 gatgtggacg ctcaccatca tgggcatatg caatttgtac gttatttatc cgcaaagcag
     5641 tttttaattt ttgcaataac agctcaggca taaagggcgt atcgcaaggc acaaaaagca
     5701 aatagtcact ttcaatttgc tcaaatcctg ccagcattcc actgagtgga ccttgaaaat
     5761 caggcaagct atcttgatag taagaaaaag caggataaag ctgctgatat tgctcaatag
     5821 aacgattgac attaagccaa atgtcactaa cttgtggtga aagtcgggct aaaatgtgtg
     5881 aaataagcgg ttttccttca aaaagttgta accctttttc taccccattc attcgacgag
     5941 ctaaaccgcc cgctaaaatc acggctgaaa tgcgttcttc tattgtttta ctcacagctt
     6001 tatcccttaa aattttgcaa aaataagagt aaaagtatat tatttcgttt aatttccaac
     6061 aagtttttta aggggtaaaa gatgaaatgc gaacgtttag aagagttatt agagttactt
     6121 ggcgaacatt ggcgtaaaaa tcctgactta cacctcattg atattttgca gcagctttca
     6181 gttgaagtgg gcgagcctga taatttcaaa gcgttaagcg atgaagtgtt aatctatcag
     6241 cttaaaatgc gaaatgcagg caaatttgag cctattcccg gcataaaaaa agattatgaa
     6301 gatgatttta aaacggcttt attgcgagct cgtggaattt taaacgatta agattgtctt
     6361 atccgttagc tagtttagca tttattattt atcaacattt ttgaaggaat cattatgaaa
     6421 aaattggcat taaaaacggc atttgttgca ctttctgccc tttttacatt taactcaacg
     6481 gcaatcgctg ctgatccaac cgcaggcaaa gaatatatcg aagtgcgtaa agcaccttct
     6541 gcacaaaaag aagtggtgga atttttctca ttctactgcc cacactgtta tgattttgaa
     6601 ttaagctaca aaatcccaag ccaaatcaaa gaaaaattac caagcgatag caaattagtg
     6661 caatatcacg ttaatttctt aggtcgccaa tctgaagatt taactcgtgc ttgggcatta
     6721 gcaatggcat taggtgcgga agataaagta aaaaccgcat tatttgaagg tgctcaaaaa
     6781 gacgcattca aatcaatgga cgatattcgc tctgtattct tagcaaacgg cattacagct
     6841 gaacagtttg atagcggtat taatagcttt gcggtaaatg gcttagtgaa taaacaagtt
     6901 caattagcgg aagacttcca aattcgtggt gtgcctgcat tcttcgtaaa tggtcaatac
     6961 caactcaact tagaaggctt tgcagattct tcatcaacga atgatttcat caaacgttat
     7021 gttgatgcgg ttgtgttctt aagcaagaaa taacccctta aaatagttag ggtttttaca
     7081 atgctgagta ggtaaatcct attcagcatt ttttatatgc aaactcggag aacaaaatga
     7141 caaagttatc tatcctaaac cttgtaccta ttcgtgaagg gcaaactgcc aaacaagcgg
     7201 tcgattctat ggtgaaatta gcaaaacacg cggaaagtat cggcattgaa cgctattgga
     7261 ttgcagagca ccacaatatg aaaaaccttg ccagctcggc aacacagctc ctcattcagc
     7321 atgccctttc tcacactgaa aaattaagag tgggatcagg tggcgtgatg ttgcctaacc
     7381 atagtcctta tattgtggca gagcaatacg gtacgcttga aacactctac cctaaccgtg
     7441 tagaacttgg gcttgggcga gcaccaggca ccgatatgcg aaccgctacc gctttacgcc
     7501 gtaatgcaca aagtctgcca tttcctgatg aaatcagcga actaaaaggt tattttgaag
     7561 gcacaaatcc tgtgtctgcc taccctgcac aggggttgaa tgtgccgttt tatattttag
     7621 gctcaagccc tgaaagtgcc tatttagcag cagaacgtgg cttgccttat gcctttgctt
     7681 cacatttcgc tcctcgtttt atggacgatg cggtgcgaat ttatcgcaca cactttaagc
     7741 cgtctgaagt gttagataaa ccctatgtga ttttaggtgt gaatgcgatt gcggcagaaa
     7801 ccgaccaaga ggcagaacag cttgctacca ctcagactca gtttttctta aatgttgtta
     7861 ccaatgccca aaactattta caaccgcccg tcgaatccga tgataaagtg tgggaaaatt
     7921 ttgtgaaagc ggaaatcgta ccgcatttcg ggcctgtttc gttccaacaa aatgcgttat
     7981 acaaccaaga gaaaaccgtt gttcgccaaa tgactgcttg ttcacttatc ggtagccgag
     8041 agagcgttga acaacagctc aagtcattaa aagcacgcat tgaaattgat gaaattatgg
     8101 cggtaagcta cattttcgat gagcaaaaac agcatcaatc ctatacttgg ttaaaagaga
     8161 ttgcagataa ggtgtgatat gagcaaaaca atgcaacaaa aagccgctga tgcacataat
     8221 atgtgtaagc taaaaggcga ctctatgtta gataactcag accgccaaat tgcctttgaa
     8281 gcagtgtatg atagtgaaga aatagctgta caagcggtcg aatttttcac acaaaaagca
     8341 aaatcggtag aaaccgagcc ttgcgacatt caaagtgaaa tcacacccat tatagatggc
     8401 tttctgatga aaatgtggat aactttcagt tgccaagcag aagtaatctt atttcaaatg
     8461 gcggtgagat agatat
//
LOCUS       DANKBV010000099         8309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.099, whole genome shotgun sequence.
ACCESSION   DANKBV010000099 DANKBV010000000
VERSION     DANKBV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.099"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(145..1149)
                     /locus_tag="PWY43_001810"
     CDS             complement(145..1149)
                     /locus_tag="PWY43_001810"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL2136672.1"
                     /translation="MAFNLKNRHLLSLVNHTEREIRFLLDLARDLKRVKYAGTEQQKL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGAHVTYIDPNSSQIGHKESMKDTARVLGRMY
                     DAIQYRGFKQSVVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIENCEKPLSQISYV
                     YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEDAFVKMCEKFAAESGARMTVTED
                     IETAIKGVDFVHTDVWVSMGEPLESWTERIDLLMPYRVTPELMQATDNPKVKFMHCLP
                     AFHNSETKVGKEIAEKYPALANGIEVTEEVFESPMNVAFEQAENRMHTIKAVMVASLA
                     "
     gene            complement(1237..2076)
                     /locus_tag="PWY43_001811"
     CDS             complement(1237..2076)
                     /locus_tag="PWY43_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL2136673.1"
                     /translation="MQSYPYLLVNVFATSHFGGNPLAVFYEADGLTDLQMQLIARQFN
                     LSEVVFIQSSTDSQAVKKLKIFTPDYEMPFAGHPTVGAAFVLHSAFDFPTTYRLQTNA
                     GLVAIQHQHNVTTFALTNGVNLENTPLSRAECASILGLNVADIASEPCYVNTGVEQLL
                     VELASQQAVEKCKINANLAEKAFLKDSVYVWCREGNEAKVRLFFQSQGSIVEDPGTGS
                     AAANLGGWHIAQGKIPIQLQISQGDEIQRPNRLLLKVDQNGKIFVGGEVILVGKGEFY
                     LPE"
     gene            2265..3599
                     /locus_tag="PWY43_001812"
     CDS             2265..3599
                     /locus_tag="PWY43_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL2136674.1"
                     /translation="MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLS
                     APPISVMLVILAVVTCASILEAAGGLKFMLQVAERILRSNPKRITFLGPLVTYLMTLM
                     LGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEIVK
                     LPGFEGVTLLNVVGVTITATFCGVIAMSLYSLRRGKELEDDPEYQRRMQDPELRKQIE
                     QTSATSLDEKLPASAKNSVYLFLAAIATIVIIAMIPAIKPTVAATGKVAGMDQIIQIV
                     MLTFGGLILILTKTDPKKVPNGIVFKSGMVATIAIFGIAWMSDTYFTHAMPAFKEGVT
                     EMVRAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAMGLPAPLLVGLMPATYAYFFI
                     PNYPSDIATVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACIVGIAVANVLI"
     gene            complement(3679..4089)
                     /locus_tag="PWY43_001813"
     CDS             complement(3679..4089)
                     /locus_tag="PWY43_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL2136675.1"
                     /translation="MNSTLTNLQPLAFLITRVVAGYMFLLHGTAKFFEFPMSMTNGNG
                     SVPLASIYGVGGILEIVGGIFFILGLFTRQTAFILSGMMAYAFFIAHGAQGNVLLPLL
                     NGGELAGVYSVLFLTYIFIGGGKYSLDAKLFNKA"
     gene            complement(4212..4889)
                     /locus_tag="PWY43_001814"
     CDS             complement(4212..4889)
                     /locus_tag="PWY43_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2136676.1"
                     /translation="MNLFGFRCFHCNHPLAIGVQGICSHCQKLLKPTPYCRHCGMSAV
                     EFRNACGYCLKNEPKWHKLVRVNEYRAPLSHWVHQFKFQQQYWWDQALARQLLLAIKQ
                     AQREQVLSLPEVIMPVPLFWHRHWKRGFNQSELLCDWLSKWLQIPLDTQSLQRVRATV
                     SQRELTAAERRKNLRGAFFYQPLKAYKRVAIVDDVLTTGSTLNVICAELLKAGVVEIQ
                     VWVLARA"
     gene            5007..5819
                     /locus_tag="PWY43_001815"
     CDS             5007..5819
                     /locus_tag="PWY43_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2136677.1"
                     /translation="MKSLQKPIKIVFFDIDDTLYVKHKAYIPKSITEQVLPRLKAKGI
                     IPAIATGRNHGCFPEALKPYLNPEIGFELFVTINGQYNFYKDQIISDYSLSREQIERI
                     IEKANALGILYAFITPMTIAVSETNPIVHEAVLPITPNYLIDPHYYEKEKIVQMLLFY
                     TEEQKQAVENSGIFANDLKEARWHPNAVDILRKENSKARGIQDVLATLNIEIESAMAF
                     GDGINDLEMLPTVGVGVAMGNGVPELKAVADFITKPLEEDGILYALETLEVI"
     gene            complement(5867..6136)
                     /locus_tag="PWY43_001816"
     CDS             complement(5867..6136)
                     /locus_tag="PWY43_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL2136678.1"
                     /translation="MGLLYLLLLNTLIGYGSYGEALNHWDTSKVSIVTTMLPIFTMIF
                     SNLSYYFYPHIFAKPDMNWISYLGALVVVSGAILAIAGDKLFRRN"
     gene            complement(6109..6786)
                     /locus_tag="PWY43_001817"
     CDS             complement(6109..6786)
                     /locus_tag="PWY43_001817"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL2136679.1"
                     /translation="MKQRPLLGFSLALTAAMTWGTLPIAAQQVLKVIDAQTLVWVRFV
                     VASVGLLLILGFAKRLPKLTACSKKELGLILLGVCGLSANFFLFAEALHYVSPTINQV
                     LWQLAPFSMILLGVLLFKEKFGLFQKIGFVLLIAGLVAFFNDKFAEILQLNTYALGVL
                     LSAGASLIWVLYGVAQKLLLAKFNSQQVLFIIYIGCSLLMLPFASPAQLGNLEDGFLW
                     GCFIYCC"
     gene            complement(6888..7145)
                     /gene="rpmA"
                     /locus_tag="PWY43_001818"
     CDS             complement(6888..7145)
                     /gene="rpmA"
                     /locus_tag="PWY43_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL2136680.1"
                     /translation="MATKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGSNVGMGKDHTLFATADGKVKFEVKGEKSRKYVSIIAD"
     gene            complement(7166..7477)
                     /gene="rplU"
                     /locus_tag="PWY43_001819"
     CDS             complement(7166..7477)
                     /gene="rplU"
                     /locus_tag="PWY43_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL2136681.1"
                     /translation="MYAVFQSGGKQHRVSEGQVVRLEKLELATGEKVEFDSVLMVVNG
                     EDVKIGAPVVAGAKVVAEVVAQGRGEKVKIVKFRRRKHSRKQQGHRQWFTEVKITGIQ
                     A"
     gene            7755..>8309
                     /locus_tag="PWY43_001820"
     CDS             7755..>8309
                     /locus_tag="PWY43_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2136682.1"
                     /translation="MAVQLFSLEQIQALANEDMQAVNAEILAQLNSEVLLINQLGHYI
                     IAGGGKRIRPLIAVLAARALGYRGKKHITAAAFIEFVHTATLLHDDVVDESDMRRGRP
                     TANAHFGNAASVLVGDFIYTRSFQMMTDLDSLRVLKVMSAATNALAEGEVQQLMNVND
                     PNTTEANYMRVIYNKTARLFEAATQ"
BASE COUNT         2413 a         1835 c         1609 g         2452 t
ORIGIN      
        1 aagacttacc caagattttt atttgaattt ttaatttact ccccgggtta gtgttttcca
       61 caaatcttct tcagctacga tgatctagcc ttaagctaaa aagcagatta ccacccaaga
      121 taatccgctt tttagactta gctattatgc caaacttgct accattaccg ctttaatagt
      181 gtgcattcga ttttctgctt gctcgaatgc cacattcatt ggcgattcaa acacctcttc
      241 ggttacttca atgccgtttg ccagtgccgg atatttttcg gcgatctctt taccgacttt
      301 agtttcactg ttgtggaacg ccggtaagca gtgcataaat ttcactttcg ggttgtcggt
      361 tgcttgcatt aattcaggcg ttactcggta aggcattaat aaatctatac gttcagtcca
      421 gctctctagc ggttcaccca ttgaaaccca aacatcagta tgcacaaaat cgacaccttt
      481 aattgcagtc tcaatatctt cggttaccgt cattcttgcc cccgattcag ctgcaaattt
      541 ttcacacatt ttcacgaatg catcttccgg taataaggct ttcggggcac aaattcgcac
      601 gtccatcccc aatttcgccc caatcaacag gagtgaatta cccatattat tgcgagcatc
      661 gccgatatag acataactga tttggctaag cggtttttcg caattttcga tcatcgttag
      721 cacgtcagcc aacatttgag ttgggtggaa ttcatcggtt aagccgttaa acaccggcac
      781 gccggcataa tcggcaagct cttgtactac cgattgttta aagccgcgat attgaatcgc
      841 atcatacatt ctgcctagca ctcgggcggt atctttcatt gattctttat gcccgatttg
      901 cgaggagttc gggtcaatat aggtaacgtg agccccttga tcgtaagctg ctacttcaaa
      961 ggcacaacga gtacgggtcg aggttttctc aaaaattaag gcaatatttt tgccttttaa
     1021 tttttgctgc tcagtgccag catatttcac ccgttttaaa tctcttgcta aatccaacaa
     1081 aaagcgaatt tcacgctcag tgtggtttac taaactgagt aaatgtctgt tttttaagtt
     1141 aaatgccatt ttgtttctcc ctgtaaacat taaaatttat tttatgcaaa ataattgcat
     1201 aaaaattcac ttatagcaaa cagaatttgc ttaccctcat tcgggtaaat aaaactcgcc
     1261 tttgccgact aaaataacct ctccgccaac aaaaatcttc ccgttttgat cgacttttaa
     1321 taacaggcga ttcggccgtt gaatttcatc accttgtgag atctgaagct ggattggtat
     1381 tttgccttgt gcaatgtgcc aaccgcctaa atttgccgct gccgaaccgg tgccggggtc
     1441 ttcgacaatc gaaccttgcg attgaaaaaa cagtcgcact ttcgcctcat ttccctcacg
     1501 acaccaaaca tatacactgt ctttcaaaaa tgctttttca gccagatttg catttatttt
     1561 gcacttttcg accgcttgtt gagaagctaa ctcaaccaat aactgctcca cgccggtgtt
     1621 tacataacaa ggctcggacg caatatccgc aacattcagc cccaatattg aggcacattc
     1681 tgcacggctt agcggtgtgt tttccaggtt cacaccattg gttaaagcaa aagtagtcac
     1741 attatgttga tgttgaatcg ctaccaatcc ggcattagtt tgcaagcgat aggttgttgg
     1801 aaaatcaaag gcggaatgca acacaaaagc cgctccaaca gttgggtggc cggcgaacgg
     1861 catttcataa tcaggcgtaa aaattttgag ttttttgacc gcttgtgaat cggtggaaga
     1921 ttgaataaat accacttcag aaaggttaaa ttggcgagca ataagttgca tttgtaagtc
     1981 ggttaatccg tccgcttcat aaaatactgc aagcgggttg ccgccaaaat ggcttgttgc
     2041 aaacacattc accagcaaat aaggatagct ctgcatttat cactccttac ataaacttaa
     2101 agccacattg ggcgaaaatt ctaccatttt ggtggcgaat cgtaaaattg gaaaatttat
     2161 cggtatccct ctttagagtt aactattttt gatttatttc aaagttttaa aaaaagatta
     2221 ggttatactt ctttctagtg attatttttt aatttgaggt tcttatgctt tatgcagaat
     2281 ttttactttt attagccttt ttatatgcag gtagccgtta cggtggcatc ggtttaggtg
     2341 tggtttccgg catcggctta tttgttgaag tgttcttctt aggaatgccg ctttccgctc
     2401 cgccaattag cgtgatgctc gtgattttgg ctgtagttac ttgtgcctcc attctagaag
     2461 ccgctggcgg tttgaaattt atgttgcaag ttgccgaacg cattttgcgt agcaacccaa
     2521 aacgtattac ctttcttggc ccacttgtca cttatctgat gaccttaatg ctcggcacag
     2581 ggcattcggt ttactctatt atgccgatta tcggtgatat tgccttaaaa aataaaattc
     2641 gcccggaacg cccgatggca gcagcctctg ttgcttcaca attaggtatt accggtagcc
     2701 cactttctgc tgcagttgcc tattacttaa ccgaaatcgt taaattaccg ggctttgaag
     2761 gggtgacttt attgaatgta gtcggcgtaa ccatcaccgc aaccttctgt ggtgtgattg
     2821 cgatgtcatt atatagctta cgtcgtggta aagagttgga agacgatcct gagtatcaac
     2881 gccgtatgca agaccctgag ctacgcaaac aaatcgagca aacgagtgca acgtctttag
     2941 acgaaaaact accggcaagt gcgaaaaact cggtttatct gttcctagcc gctattgcaa
     3001 ccattgtgat tattgcaatg attccggcta ttaaaccaac ggttgctgct acaggaaaag
     3061 ttgccggtat ggatcaaatc attcaaatcg tgatgctaac tttcgggggt ttaatcttaa
     3121 ttttaactaa aaccgacccg aaaaaagtac caaacggcat cgttttcaaa tccggtatgg
     3181 tagcaaccat tgcgattttc gggattgctt ggatgagtga tacttacttc acccatgcaa
     3241 tgccagcgtt caaagaaggg gtaacagaaa tggttcgagc ccaaccttgg acattcgcct
     3301 tcgccttgtt tgcggtatct gtagtagtaa acagccaagc cgtaaccgca aaaatgttat
     3361 taccggtagg cattgcaatg ggcttgcctg ctccattatt agttggttta atgccggcaa
     3421 cttatgctta cttctttatt ccgaattatc cgtctgatat cgcaaccgtt aactttgacg
     3481 taaccggtac aactaaaatc ggtaaatatt acttcaacca cagctttatg gctccgggtt
     3541 tagttggcgt aattactgcc tgtattgtcg gtatcgccgt tgcaaatgtg ctaatttaat
     3601 tttaacaaaa aaacaagcgg tcagattaag taaatttttt accaaatctg accgcttgat
     3661 tatttactgc aaaaccaatt aggctttgtt aaagagtttt gcatctaacg agtattttcc
     3721 gccaccgata aaaatatagg ttaagaataa taccgaatac acgcctgcta attcaccgcc
     3781 gtttaataaa ggtaatagca cattgccttg agctccgtga gcaataaaga acgcgtatgc
     3841 catcatacct gaaaggataa acgctgtttg gcgggtaaat agacctaaaa taaagaaaat
     3901 tccgcccaca atttccaaaa taccgcctac accataaatg gaggcaaggg gtacactgcc
     3961 gtttccgttg gtcatcgaca ttggaaattc aaaaaatttc gctgtgccgt gtaataaaaa
     4021 catataaccg gcaacaacac gggtgattaa aaacgctaaa ggttgtaaat ttgttaaagt
     4081 agagttcatt tgctttcctt aaaataaatt gtgatataaa ggtcaattga ttgaggcagg
     4141 cattctattt taaaaaccta tacttcacaa tccagccgtc atgaaataga ttatttacca
     4201 ttaataaaca attaggctct cgctaacacc caaacctgaa tttctactac tccagctttg
     4261 agtaactcgg cacaaattac atttaaggtg gaacctgtgg tgagcacatc atcaacaatc
     4321 gccactcgtt tataggcttt taacggttga tagaaaaatg ccccacgcag atttttccgc
     4381 cgttcagcag cggtcaattc tctttgtgag acggttgctc tcacacgttg taaactctgg
     4441 gtatccagcg gaatctgtaa ccacttcgat aaccaatcac acaataattc gctttgatta
     4501 aaccctcttt tccaatgtct gtgccaaaaa agtggtacag gcataatcac ttcaggcaag
     4561 ctcagaacct gttccctttg agcttgtttg attgccagca gcaattgtcg ggcaagggct
     4621 tgatcccacc aatattgctg ctggaactta aattgatgta cccaatgtga aagtggcgct
     4681 cggtattcat ttaccctcac taatttatgc cacttcggtt catttttcaa acaatagcca
     4741 caagcgtttc gaaactccac cgcactcatt ccacaatgac gacaataagg ggttggtttt
     4801 agcaattttt ggcaatggct gcaaatgcct tgcacgccaa tagcgagtgg atgattacaa
     4861 tgaaagcaac ggaagccgaa taaattcaca tttcctcctt ttatttgcag attatcaaat
     4921 ttagccttat tttttccggc tatttttgtg acatcgctcg aaaaaagagt aaaatctcat
     4981 tatcagtaaa taaaaaggaa accgagatga aatccctcca aaaaccgatc aaaattgtct
     5041 ttttcgatat tgatgacacc ctctatgtaa aacataaagc ctatattcca aaatcaatta
     5101 ccgagcaggt attacctcgt ttaaaagcca aaggcattat tccggccatt gccaccggca
     5161 gaaatcacgg ctgttttcct gaagctctca aaccttattt aaaccctgaa atcggttttg
     5221 agctgtttgt taccattaac gggcaatata atttctataa agatcaaata attagtgatt
     5281 attcgttaag ccgtgagcaa attgaacgta ttatcgaaaa agcgaatgct ttgggtattc
     5341 tgtatgcctt tattacgcca atgacgattg cagtttccga aaccaatccg attgtgcacg
     5401 aagcggtttt accgatcaca ccgaattatt tgatcgatcc acactactac gaaaaagaga
     5461 aaattgtgca aatgttactg ttctacaccg aagagcagaa acaagcggtc gaaaatagtg
     5521 gcatttttgc aaatgattta aaagaggctc gctggcaccc caatgcggta gatattctgc
     5581 gaaaagaaaa ttcgaaagct cgtggcattc aagatgtatt agcaaccttg aatattgaga
     5641 ttgaaagtgc gatggcattt ggcgatggta ttaacgactt ggaaatgctc ccgaccgtag
     5701 gtgttggtgt ggcaatgggt aatggcgtgc cggaattaaa agcggtggca gatttcatca
     5761 ccaagccgct tgaggaagac ggcattctat acgctcttga aactcttgag gtgatttaaa
     5821 agcaagcggt tgtaagtttt tctccattta caaccgcttg tagtttctaa ttacgcctaa
     5881 atagcttatc gcctgcaatc gctaaaatcg ccccactgac gaccaccaac gccccaagat
     5941 aactgatcca attcatatcc ggcttggcaa aaatatgcgg ataaaaatag tagctaaggt
     6001 tggaaaagat catcgtaaaa atcggcaaca tcgtggttac aatgctaacc tttgaagtat
     6061 cccaatgatt caacgcctca ccgtatgaac cgtagccaat taaggtgttc aacagcaata
     6121 aataaagcaa ccccacagaa aaccatcctc aagattgcct aactgtgccg gtgaggcaaa
     6181 cggtaacatc agcagagaac agccgatgta aataataaac agcacttgct gagagttaaa
     6241 tttcgctaat aataattttt gagccacgcc gtatagcacc caaatcaagc tggctccggc
     6301 actcaataac actccgagag cgtaggtgtt gagctgtaaa atttcagcaa atttatcgtt
     6361 aaaaaatgcg acaagccctg caatcaataa cacaaatcca atcttttgaa acaagccgaa
     6421 cttttcctta aacaataata cgcctagtag aatcatcgaa aatggagcta actgccacaa
     6481 tacttggtta atggtgggcg aaacatagtg tagagcctct gcaaacaaaa agaaatttgc
     6541 cgacagacca cacaccccta acaaaattaa accgagttct tttttgctac aagcggtcaa
     6601 tttgggtaaa cgttttgcaa atcctagaat cagcaatagc cctactgatg ccacaacgaa
     6661 cctaacccaa actaaagttt gggcatctat cactttcaat acttgctggg cggcaatcgg
     6721 tagcgttccc caggtcattg ctgcggttaa tgcaagtgaa aagccaagta aggggcgttg
     6781 tttcatcgtg gaaattcctt tttctattct atgtttctat attttagaaa gattgagata
     6841 caaaaaaacc ccgccattgg cgaggtctct tattgatttt gaaatgatta gtcagcaatg
     6901 atgcttacat atttacggct tttctcgcct ttcacttcaa atttaacttt accgtcagca
     6961 gttgcaaata aagtgtggtc tttacccata cctacatttg aacctgcgtg gaatttagta
     7021 ccacgttgac gaacgataat gctacctgct aatacagatt caccaccgaa acgtttaaca
     7081 ccaaggcgtt tagcttcaga atcacgaccg ttacgagttg aaccaccagc ttttttagtt
     7141 gccatcttct agatctcctc tgaaattatg cttgaatccc agtgattttc acttctgtga
     7201 accactgacg atgaccttgt tgtttacggc tgtgtttacg acgacggaac ttaacgattt
     7261 taactttctc gccacgacct tgtgctacaa cttcagccac tacttttgca cctgctacaa
     7321 ccggtgcacc gattttaaca tcttcaccgt taacgaccat taacactgag tcaaattcaa
     7381 ctttctcacc agttgcaagt tcaagttttt ctaaacgaac tacttgacct tcacttactc
     7441 ggtgttgttt gccgccactt tggaaaactg cgtacatatt tactccgcta tgtgccaact
     7501 gcttttgctt ttaggcacta aattaattca taaaaatagg gttgcaattc tacggaaaaa
     7561 gattcgaaag agcaagagcg attttgagaa ttttttgaaa tttgcaaaaa atggtaaaaa
     7621 tactaccgct tgtttcaact gcgactaaaa aacaatagac tgctccgatt ttacaacaaa
     7681 atcataattc ttttttgaac acaggctaaa ttaaggtaga atctactcaa ttttcgtctt
     7741 actttatgga ttcgatggct gtgcaacttt tttctttaga acaaattcaa gcccttgcga
     7801 atgaagatat gcaagcggta aatgccgaaa ttttagctca acttaattcc gaagtgctac
     7861 tgattaacca gctcggacat tatatcatcg caggcggtgg gaaacgtatt cgcccgttaa
     7921 ttgcggtgct tgcggctcgt gccttgggtt atcgtggtaa aaagcatatc acggcggctg
     7981 cttttattga gtttgtgcat actgctacac ttttgcacga tgatgtagtc gatgaatccg
     8041 atatgcgtcg tggcagacca accgcaaacg cccattttgg caatgcggct agtgtattgg
     8101 taggggattt catctatacc cgttcgttcc agatgatgac ggatttagac tctctgcgtg
     8161 tgttaaaagt gatgtctgcc gccaccaatg cgttggcaga aggggaagtt cagcaattaa
     8221 tgaacgtgaa cgacccaaac accactgaag cgaactatat gcgggtcatt tacaataaaa
     8281 ccgcccgctt gtttgaagcc gccactcaa
//
LOCUS       DANKBV010000100         8254 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.100, whole genome shotgun sequence.
ACCESSION   DANKBV010000100 DANKBV010000000
VERSION     DANKBV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.100"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            175..525
                     /locus_tag="PWY43_001821"
     CDS             175..525
                     /locus_tag="PWY43_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL2136683.1"
                     /translation="MIKYIYLAATVMILGLCGWIWHQSKQIDGLRAENQTQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKSQKIANELRNKVEVAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLHQQNNRQH"
     gene            470..694
                     /locus_tag="PWY43_001822"
     CDS             470..694
                     /locus_tag="PWY43_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136684.1"
                     /translation="MVLLIALSGCTSKTIVSTEYLYPPAAYLVPCERTAFSGKTYGDT
                     VEYLVKVMGERDLCANQIDRIREWQAEVSK"
     gene            695..952
                     /locus_tag="PWY43_001823"
     CDS             695..952
                     /locus_tag="PWY43_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018346247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136685.1"
                     /translation="MFGYKTKKQLIAEGYTHYGSLWGIPCYIGDVESEAPIIATANFI
                     PQWFLDVADWIMFTMHDLVNVNNPDAEPLMFKILLKNPIEE"
     gene            1319..1843
                     /locus_tag="PWY43_001824"
     CDS             1319..1843
                     /locus_tag="PWY43_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL2136686.1"
                     /translation="MTKKGEVKATSKGVGKLTDKQKRFVEEYLIDLNATQAAIRAGYS
                     EKTAYSIGEENLRKPEIRSAIQEAKNKRSERTQITQDDVLNGLLEVIAMSTGKKIVTE
                     TDVAKNDNGELVGFDIAKTKFEPAAANKALELLGKHLGMFKDKVDLINSDGSLNRPMV
                     IELIAPSLDDESTD"
     gene            1827..3047
                     /locus_tag="PWY43_001825"
     CDS             1827..3047
                     /locus_tag="PWY43_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDL2136687.1"
                     /translation="MKAQIELPPKLLPVFAKDYRYKGAYGGRGSGKTRAFAKMSAVSA
                     YKRAMQGESGVILCGREFMNSLEESSLEEVKQAIKSEPFLANFFDVGEKYVRTKDGRI
                     SYVFSGLRHNLDSIKSKARILIAWIDEAETVSETAWRKLIPTVRENNSEIWVTWNPEN
                     RGSATDTRFRQNPPENSCIIEMSYQDNPWFPDVLEQERLNDKQRLDDATYRWIWEGAY
                     LEASEAQIFRGKYQEMEFVPNPDFDGPYYGLDFGFAQDPTAAVKCWVFNGDLYIEFEA
                     GKVGLELDDTADFIAKGVPQIADHVVRADSARPESISYLKRHGLPRIIGVDKWKGSVE
                     DGIEHIKSYGKVFIHPRCQQTLNEFRLYSYKTDRLSGDVLPVVIDAHNHFIDALRYAL
                     TPLMQVKSAKGVLL"
     gene            3044..4420
                     /locus_tag="PWY43_001826"
     CDS             3044..4420
                     /locus_tag="PWY43_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDL2136688.1"
                     /translation="MSLEQDRLAFLAEALGLGNIKRRTLWKEFGYPNTLTFNHFLKAY
                     KRNSIAFAAINRLLDGCWVDCPVIVEGEQKNESKQTTEWESKVERFMKRYWSAIKEAD
                     RRNLVGNYSALLLQVRDGQQWDQPILQGALSSIGEFGLVKLIPVWQSQLSVTEFQEDV
                     TADNYGEPLMYQFSESAFGKKSVSRNIKVHASRVILLNEGGDFNSPHSGVPLLEAGYN
                     KLVDLEKTSGGSAEGFLKNASRQLGIKLTKDVDLRQLEDSAKALGFSSFSDALNDKIK
                     KINSGTDSALITHEGDASVLSVAPADPKPTWEISANEFAASVQIPFTILFGQQTGRLA
                     SDEDKTDWANRCNGRRNGFLTDVITQLLNRLWFIGVLPMPKNSDVTVSWSDLLAPSEK
                     EKIANAQALASVATTSQSAFGFAAVTANEIRESLGFEPLPDNLIPPTIDDENEDQDEN
                     QATADSDK"
     gene            4374..5312
                     /locus_tag="PWY43_001827"
     CDS             4374..5312
                     /locus_tag="PWY43_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL2136689.1"
                     /translation="MKIKTKIKPLRIPTNKADPLNMGVSVAKLFRHIDVIYSQIKRSI
                     KQQILQKMSLRLQANNFEYFPSYLTATELADLLETLQKIIDKEILSNGSQGENLWFDS
                     YIDEAALKGTQSAVTDLGLQSEAYRSQRNLSSIVFSPAYFNRLAIAHMASYSEWQGLS
                     DELRKSLAGVITEAVLNGKNVKETAREIRQKLDVSSKRAKRIAQSSQLAAYRRAEWQE
                     AEEAKEELGLNTKLLHFSALKATTRLAHAQRHGKYFDVAEVREWYSRDGNQFNCYCKQ
                     SVVVVNADGKTDIEPLLVGLAKERKQWVGALKNVKK"
     gene            5299..6669
                     /locus_tag="PWY43_001828"
     CDS             5299..6669
                     /locus_tag="PWY43_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDL2136690.1"
                     /translation="MSKNNVNIVTVINSKNISTESIDGDQHLIIRGVVPIVDDVVMND
                     GLYPADEINKSYNSLEGNFMPLGHPKIEGKYVSAQDVRAVNKHHVGAWAKNVRKESGK
                     VLVDMYINRRFAEGSEQGKTLLERLDGMVTNTNAEPIHVSTGLILNKVKQSGKSKGKK
                     YSWIATNMSFDHIAILLDVPGAATPDDGVGIFVNQDGSEMGVENVNLTQAADYRTEKL
                     FDKVRFYLMANSDLSFEEIHRALREAIRPEGKDKWSFWVEAVYPKYFIYSDDNSGKKY
                     KRSYFIDENDNVQLTSEPVEVVKKIGYEEITTNEEHNPMKTKILAVLNAANVKTEGLN
                     DDQLLEAYNKLQADKGKEPEKPKNEDDSEIDEKIKKAVNAAIEPLQQALQANTDKEQA
                     AMRAAVKAKFNMSDAAVNALQGEVLTELYAQTQQSVGLNNNSANGNGDNQWDGYSLNQ
                     EEKGNE"
     gene            6662..7096
                     /locus_tag="PWY43_001829"
     CDS             6662..7096
                     /locus_tag="PWY43_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136691.1"
                     /translation="MSNVIYRGSVKREPQTVNIVINDTSAPGAVVKLHNGKLEAAADL
                     KGRRFLLGNNRFKGQTIEQAYAKGDTATAFRLEPEQEYYAQIADGTYNFGDELTVKQA
                     GGKLTKAETGDVVLFFFDEESQRVISGGKGYADVVVANAYTK"
     gene            7111..8097
                     /locus_tag="PWY43_001830"
     CDS             7111..8097
                     /locus_tag="PWY43_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HDL2136692.1"
                     /translation="MLKFTKEQERFVINERINYDKKHALMAANSAGLLGNAMTLPKDV
                     WAEWDRDAVQIQREELVVFNDLAGISKSMPIGKLIHHFQTVSDSGSVNISLDGRSSAK
                     TDNVVLNYHGTPLPIIDSTFSFGWRDMSAAQSEGYQIDGAARANSLRKVAEKMEDLAL
                     NGDSSIVVGNAKLYGLRTAPNRMTNTHNLDLATATPKEIYDVFRDLISKFHAKNYYSP
                     VTLYVNYGDYFAMSTRDYSEQKSESILNKVMTIPQIAKIVPASRVPQNEILGLCKRSD
                     VYQVLNGMPLVTRPIARHNETDDYSFQIMAAVAVEFKFDDKGNAGYIQYTKS"
     gene            8109..>8254
                     /locus_tag="PWY43_001831"
     CDS             8109..>8254
                     /locus_tag="PWY43_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136693.1"
                     /translation="MKWLLTHDSHELKKGDVYEGETLPLWLVGKAIEIEEKSFEVATP
                     SDHA"
BASE COUNT         2547 a         1669 c         2010 g         2028 t
ORIGIN      
        1 cccgaccaaa tcactaaaaa tctagcattg acagtacagg tggctgtttg gtactgggta
       61 agtcgcaagt tatccgaatt tagcgacatt cttactgtta ctaaaaagat taatggcgga
      121 acgcacggtc taactcagcg tacagcttat tataaaaagc taatggcgga ctaaatgatt
      181 aaatacattt atctagcggc cacagtaatg attttgggtt tgtgtggctg gatttggcat
      241 cagtccaagc agattgatgg cttaagagcc gaaaaccaaa cacaagccca aaccattgag
      301 cagcagcaaa aagccaatca acggctaacc gatagcttag agcaagagcg acaagcggtc
      361 gaaaaaagcc aaaaaattgc aaatgagctg cggaataaag tggaggtggc caagaatgaa
      421 attacatcaa tactggcaca agacagttgt gctaaggctg atttgcctaa tggtgttgct
      481 gatagcatta agcggttgca ccagcaaaac aatcgtcagc actgagtatt tatatccgcc
      541 tgcggcttac cttgtgccgt gcgagcggac agcatttagc ggtaaaacct acggcgatac
      601 ggtcgagtac ctcgtcaaag taatgggaga acgtgatttg tgtgctaatc aaatcgaccg
      661 tatcagagaa tggcaggcag aggttagcaa ataaatgttc ggatataaaa caaaaaaaca
      721 gttaattgcg gaaggataca cgcattacgg cagtttgtgg ggcatacctt gttatattgg
      781 tgatgtagaa agcgaagctc ccataatcgc tacagctaat tttattccgc aatggttctt
      841 agatgtagct gattggatta tgttcacaat gcacgactta gtaaatgtga ataatcctga
      901 tgcagagccg ttaatgttta agattttact gaaaaatcca atagaagaat gattacagag
      961 gagcttaaac gctccttttt tattttaatg aaaagtaaat gctacataat ttagctatgg
     1021 tttcggggtt ggttggataa aacaccgagg aaatacctaa gtgatgtgat ttatataaaa
     1081 caaaaccccg aacatttgcg gtgttcgagg ttttttatta cccattgaaa cggaatgagt
     1141 aacaagttat ggaaattatt acatttttaa ccgtaaccat caaggagatt cttatggaat
     1201 atggtttatg gcaaataagc ctagcagtaa cagtccctat tttggcgttc gtgtcgccaa
     1261 agctgattaa tgctattgct aatctattaa atgcaatgaa gtaaaggaat ttaacccaat
     1321 gactaaaaaa ggcgaggtta aagccacgtc taaaggcgtg ggtaaattaa ctgataaaca
     1381 gaaacgattt gtagaagagt atctgattga cttgaatgcg actcaagctg cgattagagc
     1441 tgggtatagc gaaaaaacag catactcaat cggagaagag aacctgagaa aacctgaaat
     1501 cagaagtgca attcaggagg ctaaaaataa acgctcggag cgaactcaaa ttacacaaga
     1561 tgatgtgcta aatgggttgc ttgaagttat tgcaatgagt actggtaaaa aaattgtgac
     1621 agaaactgat gttgcaaaaa atgacaatgg tgagttggtc ggttttgata ttgcaaaaac
     1681 aaaattcgaa cctgccgccg caaataaagc tcttgagtta ctaggtaaac atttaggaat
     1741 gtttaaagac aaggttgatt taattaattc tgatggttca ctgaatcgac cgatggtgat
     1801 tgaattaatt gctccgagtt tagacgatga aagcacagat tgaactacca cctaaactgt
     1861 tgcctgtttt tgccaaagat tatcgttaca aaggtgctta tggtgggcga ggttcaggca
     1921 agactcgggc atttgccaaa atgtcggcag tgtctgctta taaaagagca atgcaagggg
     1981 aaagtggcgt tattctttgc ggtcgtgagt ttatgaactc attggaagaa agctctcttg
     2041 aagaagttaa acaggcgatc aagtctgagc cttttttagc aaattttttt gatgtcggcg
     2101 agaagtatgt tcgcactaaa gatgggcgga tttcttatgt gttttccgga cttcggcata
     2161 acttagatag catcaaatct aaagcccgta ttttgattgc gtggatagat gaagcagaaa
     2221 cagtaagcga aactgcttgg cggaaattaa ttccaaccgt gcgtgagaat aattccgaaa
     2281 tatgggtaac ttggaacccg gaaaatagag ggagcgcaac agatactcgc ttcaggcaaa
     2341 atccacctga aaacagctgc ataattgaaa tgagctacca agataatcct tggtttcctg
     2401 atgtgctcga gcaagagcgg ctaaatgaca aacagcggct tgatgatgcg acttatcgct
     2461 ggatttggga aggtgcttat ttggaagcga gcgaagcgca aatctttcgc ggtaaatatc
     2521 aagaaatgga atttgtgccg aaccctgatt ttgatggccc atattacggc ttagattttg
     2581 gtttcgcgca agatccgacg gcggcggtca aatgctgggt atttaatggc gatctgtaca
     2641 tcgagtttga agcaggcaaa gtgggcttag aacttgacga caccgcagac tttattgcca
     2701 aaggcgtgcc acaaattgcc gatcacgttg tgcgtgcaga ttcggcgcgt cctgagtcaa
     2761 ttagttacct caaacgtcac ggcttaccgc gcattatcgg tgtggataag tggaaaggct
     2821 cggttgaaga tggcattgag catattaagt cctacggcaa agtctttatt cacccacgct
     2881 gtcagcaaac gctcaacgaa tttcgccttt attcgtacaa aaccgaccgc ttgtcgggcg
     2941 atgtattgcc tgtcgtgatt gatgcccata accactttat tgatgcgcta cgctacgcct
     3001 taacgccgtt gatgcaagtt aaaagtgcca aaggagtttt actatgagcc tagagcaaga
     3061 cagactggct tttttagccg aagccctagg gcttggcaat atcaaacgaa gaacgttatg
     3121 gaaggagttt ggctacccta ataccttaac attcaaccat ttcttaaaag cctataagcg
     3181 caatagtatc gcttttgcag cgattaaccg cttacttgac ggttgttggg ttgactgccc
     3241 cgtaatcgtt gagggtgagc aaaagaacga atcaaagcag acaaccgaat gggagtccaa
     3301 agttgaacgt tttatgaaac gctactggtc ggcaatcaaa gaggcagata gacgaaacct
     3361 tgtcggtaac tattcggcgt tattacttca agtaagagat ggtcaacaat gggatcagcc
     3421 tattttgcaa ggcgcgctat ctagcattgg agagttcgga ctggttaaac tgatccccgt
     3481 gtggcagtca cagctttcag tgacagagtt tcaagaggat gtgaccgccg ataattacgg
     3541 cgaaccgctg atgtatcaat tcagcgaatc tgcctttggc aaaaaaagcg tgtcacgaaa
     3601 tatcaaggtt cacgcaagtc gtgtaatttt gctcaatgag ggtggcgact ttaattcccc
     3661 tcattctgga gttcctctgc ttgaggcggg ttacaacaag ctagttgatc tcgaaaaaac
     3721 atcgggcggc agcgccgaag gttttctgaa aaacgcaagc cgacaattag ggatcaaatt
     3781 aacaaaagat gttgatttga gacagctcga agacagcgca aaagcccttg ggttttcttc
     3841 attttctgat gcgttgaacg acaagatcaa gaaaatcaac tcaggaacag actcagcctt
     3901 gattactcac gaaggtgatg cctctgtgtt gtcagtcgca ccagcagatc cgaagccaac
     3961 gtgggaaatt tctgcaaatg agtttgccgc ctcagtacaa atccctttca cgattttgtt
     4021 cggacagcaa acagggcgat tggcaagtga cgaggacaaa accgactggg caaaccgttg
     4081 caacggtcgt cgaaatggtt ttctcaccga tgtgataaca caactactta atcgcttgtg
     4141 gttcatcggt gtgctaccta tgccaaaaaa tagcgatgtc acagtgtctt ggtctgactt
     4201 acttgcacca agcgaaaaag agaaaatcgc aaacgcacag gctttggctt cagttgcaac
     4261 tacatcgcag tctgcctttg gttttgccgc ggtaacagcg aacgaaatcc gcgaatcttt
     4321 agggtttgaa cctctgcctg ataatctgat tccgccaaca attgacgacg aaaatgaaga
     4381 tcaagacgaa aatcaagcca ctgcggattc cgacaaataa agccgatccg ctcaatatgg
     4441 gagtgtcggt tgccaagcta tttcgtcaca ttgatgtcat ttactcacaa attaagcgct
     4501 ccattaagca acaaatcttg caaaagatgt cactgcgact gcaagcaaat aattttgagt
     4561 attttccttc gtatcttact gcgactgagt tggcggattt gcttgaaacc ttgcaaaaga
     4621 ttatcgacaa agagattttg tcgaatggtt ctcaaggcga aaatctctgg tttgactcct
     4681 atatcgacga agcagcacta aaaggcactc agtctgcggt cactgattta ggtctgcaat
     4741 cagaggctta tcgcagtcag cgaaacttat catcaatcgt tttcagtcct gcgtacttca
     4801 atcgattggc gattgcacac atggcaagtt atagcgagtg gcaaggttta agcgacgagt
     4861 tgcgaaaatc tttggcgggg gtgatcacgg aggcggtttt aaacggcaaa aacgtgaaag
     4921 aaactgcccg tgagattcgg caaaaacttg atgtgtcatc aaaacgggcg aagcgaatag
     4981 cgcaatcaag ccagttggcg gcatatcgtc gggcagaatg gcaagaggcg gaagaggcaa
     5041 aagaagaact tgggctaaac accaagctct tacatttttc agcgctcaaa gctactacgc
     5101 gattagcaca cgcccaacgc cacggcaaat acttcgatgt tgcagaggtg cgcgaatggt
     5161 atagccgaga cggcaaccaa ttcaactgtt actgcaagca gtctgtggtt gtggtcaatg
     5221 ccgacggtaa aacagatatt gagcctctcc ttgttgggtt ggctaaagaa cgtaaacaat
     5281 gggtaggagc attaaaaaat gtcaaaaaat aacgtgaaca tcgtcaccgt cattaacagc
     5341 aaaaatatca gcacagaatc tattgacgga gatcaacacc tgattatccg cggcgttgtg
     5401 ccgattgttg atgatgttgt gatgaacgac ggactttatc ctgcggatga aatcaataaa
     5461 agctacaaca gcttagaggg caactttatg ccgcttgggc atccaaaaat cgaaggtaaa
     5521 tacgtttcag cccaagatgt tcgagcggtg aacaaacatc acgtcggtgc gtgggcgaag
     5581 aacgtacgca aggaaagtgg caaagtgctg gtcgatatgt atatcaaccg ccgctttgcg
     5641 gaaggttcag aacagggcaa aaccttgctt gaacgacttg acggaatggt gacaaacacc
     5701 aacgctgaac ctattcacgt ttccacaggg ctgattctaa acaaagtcaa gcaatcgggt
     5761 aaatccaaag gcaagaaata tagctggatt gcgacaaata tgtcgttcga tcatattgca
     5821 atattgcttg atgtgccggg cgcggcgaca cctgatgatg gcgtggggat ttttgtaaac
     5881 caagacggaa gcgaaatggg ggtggaaaat gtaaacctaa cacaagcggc ggattaccgc
     5941 acagaaaagc tgtttgacaa agtacgattt tacctgatgg cgaactcaga tttatcgttt
     6001 gaagaaattc atcgcgcgct gcgtgaagct attcgccccg aaggcaaaga taagtggtca
     6061 ttttgggtcg aagctgttta cccgaaatac ttcatctatt cggacgataa cagcggcaag
     6121 aaatacaagc ggagctattt tattgacgaa aatgacaatg tgcaactcac tagcgaacct
     6181 gtcgaagttg tcaagaagat tggctacgaa gaaattacaa ccaatgagga acataatcca
     6241 atgaaaacca aaatcttagc cgtgttgaat gcggcaaacg tgaaaacgga aggcttaaat
     6301 gacgatcagc tcttggaagc ctacaacaag ctgcaagcgg ataagggtaa agagcctgaa
     6361 aagcctaaaa atgaagatga cagcgaaatt gatgagaaaa tcaagaaagc agtcaatgcc
     6421 gcgattgagc cactccagca agccttgcaa gctaatactg acaaagagca agcagcaatg
     6481 cgtgcggcgg tgaaagccaa gttcaatatg agcgatgccg cagtcaatgc tttgcaaggc
     6541 gaggtattaa ctgagttgta tgcgcaaacg caacaatcgg tcggattaaa caataattcg
     6601 gccaacggca acggcgacaa tcaatgggat ggttactcac tcaaccaaga ggagaaaggc
     6661 aatgagtaat gtaatttatc gcggttcggt caagcgtgaa cctcaaacgg tgaatatcgt
     6721 gatcaacgat acttcggcac cgggtgcagt agttaaactc cacaacggca aattagaggc
     6781 ggcggctgat ttaaaaggtc gccgtttttt attgggcaac aaccgcttta aggggcagac
     6841 aatcgaacaa gcgtatgcga agggcgatac tgcaacggcg tttcgtttag agcctgaaca
     6901 ggagtattac gcgcaaattg ccgacggcac ttataacttt ggcgatgaac tcacggtaaa
     6961 acaagcgggc ggcaaactga ccaaagcgga gacgggcgat gtagtgctat ttttcttcga
     7021 cgaagaatcg cagcgtgtaa tcagtggcgg caaaggctat gccgatgtcg tggtggcaaa
     7081 cgcctacact aaataagagg taacaaagca atgttgaaat tcacaaaaga acaagaacgc
     7141 ttcgtgatta acgagcggat caattacgac aaaaaacacg cgctaatggc agcaaattcg
     7201 gcggggttat tgggcaacgc gatgactttg ccgaaagatg tctgggcgga atgggatcgt
     7261 gatgcagtgc aaattcagcg cgaagaattg gtggtgttta acgatttagc gggcatttca
     7321 aaatcaatgc cgattggcaa acttattcac catttccaaa cggtatctga tagcggttcg
     7381 gttaatatct cattagacgg tcgttcatca gcgaaaaccg ataacgtggt gcttaactac
     7441 cacggtacac cgttgccgat tattgattcc actttctctt ttggctggcg cgatatgtcg
     7501 gcagctcaaa gcgaagggta tcagatcgac ggtgcagcac gcgcgaacag tctccgcaag
     7561 gtggctgaaa aaatggaaga tctcgcttta aatggtgatt cctctattgt ggttggcaat
     7621 gccaagcttt acggcttgcg tactgcgcca aatcgtatga ctaacacaca caatctggat
     7681 ttagccactg cgacaccgaa agaaatttac gatgtgttcc gtgacttgat tagcaaattc
     7741 cacgcgaaga actactactc tcctgtaacg ctttatgtga actatggcga ctatttcgca
     7801 atgagtacac gtgattactc cgagcagaaa tccgaatcta tcttgaacaa agtgatgacg
     7861 attccgcaaa ttgcgaaaat cgtgccagca agccgtgtac cgcaaaatga aattctcggc
     7921 ttgtgcaagc gttcggatgt gtaccaagtg cttaacggta tgccgttagt gactcgtccg
     7981 attgcgcgcc ataatgaaac cgatgattac tcgttccaaa ttatggcagc cgttgccgtg
     8041 gagttcaaat tcgacgataa aggcaatgct ggctatatcc agtacactaa atcctaaggc
     8101 ggtgcaaaat gaagtggcta ttaacacacg atagccacga gttgaagaaa ggcgatgttt
     8161 acgaaggcga aacattaccg ctttggcttg tgggcaaagc tatcgaaatc gaagaaaaat
     8221 cctttgaagt tgcaacgcct tctgatcacg ctga
//
LOCUS       DANKBV010000101         8153 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.101, whole genome shotgun sequence.
ACCESSION   DANKBV010000101 DANKBV010000000
VERSION     DANKBV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.101"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(237..542)
                     /locus_tag="PWY43_001832"
     CDS             complement(237..542)
                     /locus_tag="PWY43_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136694.1"
                     /translation="MSKLNKLLSRKTSRKIIPEQVNFDINLPIYIPLFLSDKTAKIQA
                     VWELEQENGERDAGAVKRNRIQFRYLPEGVHKLTLIVGTPLLSKGSKVYQCLIKVNK"
     gene            917..2032
                     /gene="asd"
                     /locus_tag="PWY43_001833"
     CDS             917..2032
                     /gene="asd"
                     /locus_tag="PWY43_001833"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL2136695.1"
                     /translation="MQNVGFIGWRGMVGSVLMDRMVEENNFANINPVFFTTSQAGQKA
                     PVFGGKDAGELKNAFDIEELKKLDIIVTCQGGDYTNEVYPKLKETGWNGYWIDAASAL
                     RMKDDAIIVLDPVNQHVISEGLKNGIKTFVGGNCTVSLMLMAIGGLFEKDLVEWVSVA
                     TYQAASGAGAKNMRELLSQMGELEDSVKAELANPASSILEIERKVTAKMRAEDFPTEN
                     FGAALGGSLIPWIDKLLPETGQTKEEWKGYAETNKILGLSDNPIPVDGLCVRIGALRC
                     HSQAFTIKLKKDIPLAEIEQIIAAHNEWVKVIPNDKETTLRELTPAKVTGTLSVPVGR
                     LRKLAMGGEYLAAFTVGDQLLWGAAEPVRRILVQLTQ"
     gene            2142..3068
                     /locus_tag="PWY43_001834"
     CDS             2142..3068
                     /locus_tag="PWY43_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2136696.1"
                     /translation="MKPIFLEIRHLRTLIALKESGSVSLAAKRVHLTQSALSHQIKLL
                     EEQYDLTLFERKSSPIRFTPAGERLIKLAYDILPKVIEAELDLARVKQGEVGEVRVAV
                     ECHTCFDWLMPAMDTFRQHWPLVELDIVSGFHTDTVGLLLSHRADLAIVAEEEDSAGI
                     IYKPLFSYEMVAICSKDHPLATKDVWEAEDFKDETLITYPVPDDMLDLLRKILGPAGI
                     KVTRRNSELTIAIIQLVASRRGIAALPYWAVKPYLDRGYVVARKITQQGLYSTLYGAI
                     READATLAYLDDFHDTVKSQSFATLPGLMVLE"
     gene            complement(3135..4580)
                     /gene="pntB"
                     /locus_tag="PWY43_001835"
     CDS             complement(3135..4580)
                     /gene="pntB"
                     /locus_tag="PWY43_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDL2136697.1"
                     /translation="MSFGFVTAAYIIAAILFIMSLAGLSKHETAKAGCWYGIVGMAIA
                     LVATIFGPQSHGTIWILIAMAIGGFIGVKKALKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYGLHTNALMPANLDETAQAAFLAEQATLANIHNVEVFLGIFIGAVTFSGSLVAFG
                     KLSGKLFGRKVSSAALNIPHKHKWNLAAIIVSVFLMIVFLNNPHNIFPVLIMTIIALV
                     FGWHLVSSIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYI
                     MCKAMNRSFISVIAGGFGNEVQSGKGDEEYGEYRETTAEEVAEMLKNASSVIITPGYG
                     MAVAQAQYPVAEITAKLREKGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDE
                     INDDFEETDVVLVIGANDTVNPAALDDPSSPIAGMPVLEVWKAQNVIVFKRSMAVGYA
                     GVQNPLFFKENTQMLFGDAKERVDDILRALN"
     gene            complement(4595..6133)
                     /gene="pntA"
                     /locus_tag="PWY43_001836"
     CDS             complement(4595..6133)
                     /gene="pntA"
                     /locus_tag="PWY43_001836"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDL2136698.1"
                     /translation="MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEQDAGFKAS
                     FEDNAFLNAGANIGTQQEVWNADIIFKVNAPTEAEIELIKEGVTLVSFIWPAQNPQLM
                     EKLQSKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
                     GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEE
                     EGGSGDGYAKVMSEEFNRRAMALYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMK
                     PGSVIVDLAAATGGNCEYTKAGEIVVTDYQVKVIGYTDFPARLPTQSSQLYGTNLVNL
                     LKLLAPNKDGQIEINFDDVVLRGVTVVRDGELTWPAPPIQVSAQPQKQAAAPQAVKKE
                     EKPADPRVKYGVMAAAGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHT
                     PLMAVTNAISGIIIVGAVLQIAQPTGNFFIDILAFIAILVASINIFGGFKVTQRMLAM
                     FRKG"
     gene            6313..7029
                     /gene="trmJ"
                     /locus_tag="PWY43_001837"
     CDS             6313..7029
                     /gene="trmJ"
                     /locus_tag="PWY43_001837"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDL2136699.1"
                     /translation="MQILNQIQIILVETSLPANIGSAARAMKTMGLTHLRLVAPKQPL
                     DEQAEALAAGAKDVLDNAQCYATFDEAIADCQLVIGTSARLRHLQNTLIEPRDCGELV
                     VNRANLGKVAIVFGRERVGLTNEELLKCQYHLNFPTNPDYGSLNLAMAVQLASYEIRM
                     AYLQNSGEKQPLAADKDYPTAQALEHFFAHTERLYTELGFIRNDSVMLKLRRLYQRAE
                     LETNELNLLRGMLTAVEKAN"
     gene            complement(7116..>8153)
                     /locus_tag="PWY43_001838"
     CDS             complement(7116..>8153)
                     /locus_tag="PWY43_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDL2136700.1"
                     /translation="NKWLTDLSELEKFNVFVDDADVQAQVAAVKVENKRRLAKYVEET
                     QGIKLNPEAIFDVQVKRIHKYKRQQMNVLHIITLYNRILKNPTADWTPRVFIFAGKAA
                     SAYYAAKKVIRLINDVANVVNNDPRINDLIKVVFIPNYGVSLAQMIIPAADVSEQISL
                     AGTEASGTSNMKFALNGALTIGTLDGANVEILDHVGKENIFIFGNEVEQVEELRRNGY
                     SPYHYYEKDSELNEAISQILNGKFSPEDPYRYQDLILNSGDYYQACADYRSYVEAQEK
                     VAQAYRNKKAWTRSAIINIANMGYFSSDRSVMDYAKNIWHIEPMSEEQLKGDSTMGSI
                     IESSNKLLNLK"
BASE COUNT         2456 a         1753 c         1551 g         2393 t
ORIGIN      
        1 taaatatttc aaggctttta ttatttcata aaaataaaca taaataaatg tagtggtaaa
       61 atcaaaattt taaaaatatt gtataaataa tatattatac ttctttctaa attactatgt
      121 tgacgtaatt tatctttgat ttcttttaaa ctaccctcac cagggtgttt gctattttta
      181 aaatggtttc tgttttacaa tatacacata aatacgatca caacataaag ttaatattat
      241 ttattaactt taattaggca ttgataaacc ttacttcctt ttgataataa aggcgtacca
      301 acaattaatg ttaatttatg taccccttct ggcaagtatc taaactgaat acgatttcgc
      361 tttacagcgc cagcatcacg ctcaccattt tcttgttcta actcccatac tgcttgaatt
      421 ttcgctgtct tatctgataa aaacagcggg atatagatag gcaagttaat gtcaaaatta
      481 acttgctcag gaataatttt acgcgatgtt tttctcgata ataatttgtt taacttagac
      541 atagaatgtt cctttgtaaa atgtaaaata aactcattca ttactgtaaa gcttaaaata
      601 aaagtattag aaataatagt taaaaacctc ccatttaact aacctagatc aaactttagg
      661 taattacatc tacttcctat cgatatatcg gcaaaattta ttgattagac cacaattgga
      721 atgcataaat tgtgagcatt tccctattta taagattcta tttatttcca aaaatacacc
      781 ataatcgatg ttttatttta acaatagcgg ttaaattttg tcaaaacttt gcaaatcaat
      841 aaaaaacatt gatttttgca ctaaaatcct gtatttattc acaatcttta taacatcatt
      901 tttaggagca aacacaatgc aaaatgtagg ttttatcggt tggcgtggta tggtcggttc
      961 ggtcttaatg gatcgtatgg ttgaagaaaa taacttcgcc aatatcaacc ctgttttttt
     1021 cactacttca caagcgggtc aaaaagcccc tgtttttggc ggaaaagatg ccggcgaatt
     1081 aaaaaacgcc tttgacattg aagaattaaa aaaattagac attatcgtaa cttgccaagg
     1141 tggtgattat acaaatgaag tctatccaaa attaaaagaa accggttgga acggctattg
     1201 gattgatgcg gcttctgcat tacgtatgaa agacgatgct attatcgtat tagacccggt
     1261 gaaccaacat gtgatttcag aaggcttaaa aaacggcatt aaaacatttg taggcggaaa
     1321 ctgtaccgta agcctaatgc taatggctat cggtggttta tttgagaaag atttggtcga
     1381 gtgggtttct gtagcaactt accaagccgc ttccggtgcc ggtgcgaaaa atatgcgtga
     1441 attgctttct caaatgggtg aattagaaga tagcgtaaaa gctgaattag caaatcctgc
     1501 ctcttctatt ttagagattg aacgtaaagt tacggctaaa atgcgtgcgg aagacttccc
     1561 aaccgagaac ttcggtgcgg cattgggcgg tagcttaatt ccttggattg ataaattatt
     1621 accggaaacc gggcaaacta aagaagagtg gaaaggttat gcagaaacta ataaaatttt
     1681 aggtttaagc gataacccaa ttccagttga tggtttatgc gtgcgtattg gagcattacg
     1741 ctgccacagc caagcattca caattaagct gaaaaaagat attccgttag cggaaatcga
     1801 gcaaattatt gctgcccaca atgaatgggt gaaagtaatt ccaaacgaca aagaaaccac
     1861 attgcgtgaa ttaaccccgg cgaaagtgac cggtacattg agcgtgccgg ttggtcgctt
     1921 gcgtaaatta gcgatgggtg gagaatactt agctgcattt accgtaggtg accaattatt
     1981 atggggtgca gcagagcctg ttcgccgcat tttagttcaa ttaacccaat aattcgccac
     2041 tgctctatac aagcggttgg attttgcaaa ttttttgcag aatttgaccg cttgtttgtt
     2101 ataattattt tcatcttttt tcacttttag atgaataaat tatgaaaccg atttttcttg
     2161 aaatccgcca tttgcggaca ttaatcgcat taaaagagag cggtagcgta tctcttgcag
     2221 caaagcgtgt ccatttaaca caatccgcac tttcacacca aataaaatta ctggaagaac
     2281 agtatgatct tacgctattt gagcgaaaaa gctcccctat tcgctttaca ccagctggcg
     2341 aacgtttaat taagttggcc tacgatattt tgcccaaagt cattgaggct gaattagatt
     2401 tagcccgagt gaaacaagga gaagtcggag aggtacgtgt tgcagtagaa tgccatacct
     2461 gttttgattg gctaatgccg gcaatggata ctttccgtca acattggcct ctagttgaat
     2521 tagatatcgt atcgggtttc catactgata cggttggttt gctattaagc catcgtgcag
     2581 atctggcaat tgtcgctgaa gaggaagata gtgctggtat tatatacaaa cctctctttt
     2641 catatgaaat ggtagctatt tgctctaaag atcaccctct tgccacaaaa gatgtatggg
     2701 aagctgaaga ttttaaagat gagacattaa ttacctatcc agtacctgat gatatgctag
     2761 atttactgcg aaaaatatta ggccctgctg gcatcaaagt tactcggcgt aatagtgagt
     2821 taactattgc tattattcag ctagtagcaa gtcggcgagg cattgctgca ctaccttatt
     2881 gggcggttaa accatattta gatagggggt atgtggtggc aagaaaaata actcagcaag
     2941 gactatatag caccctttat ggcgctattc gagaagccga tgctacccta gcctacttag
     3001 atgatttcca tgataccgta aaatcgcaga gttttgcgac tcttcccgga ttgatggtat
     3061 tagagtaaaa aattaacaat ttatgttagc ctaagcacaa aaaagcgaac ttatgttcgc
     3121 ttttttattt aaatttaatt taacgcccgt aaaatatcat caacacgttc ttttgcatca
     3181 ccaaatagca tttgggtatt ttctttaaag aacagtgggt tttgcacacc ggcgtagcct
     3241 actgccattg aacgtttgaa cacgataaca ttttgtgctt tccatacttc aagcaccggc
     3301 attccagcaa tagggcttga tgggtcgtct aatgcagcag ggtttacggt atcgtttgca
     3361 ccgattacca ataccacatc ggtttcttca aaatcgtcat tgatttcgtc catctccagc
     3421 actacatcat aaggtacttt cgcttccgcc aataatacgt tcatatgccc cggtaaacgc
     3481 cctgccacag ggtgaatacc aaaacgcaca ttcacgcctt tttcacgcaa ttttgcggtg
     3541 atttccgcta ccggatattg tgcttgagcc actgccatac cataacccgg cgtgatgatc
     3601 acagagcttg catttttcag catttctgcc acttcttcgg ctgtcgtttc acggtattca
     3661 ccgtattctt catcaccttt gccagactga acttcattgc caaaaccacc ggcaatcacg
     3721 ctaatgaatg agcggttcat tgctttacac ataatataag aaaggatagc acctgatgat
     3781 cccactaatg caccggttac gattaataaa tcgttgctta gcataaagcc tgccgctgcg
     3841 gctgcccaac ctgaataaga attcagcatt gaaactacca ccggcatatc cgccccacca
     3901 atagaagaaa ctaagtgcca gccgaagact aacgcaataa ttgtcataat cagcacaggg
     3961 aagatattgt gcggattatt taaaaacacg atcatcaaaa aaacagaaac gataatggct
     4021 gccaaattcc atttgtgttt atgtggaata ttcagtgctg cagaagagac tttacgacca
     4081 aataatttac cgcttagttt accaaaggca accaatgaac cggagaaggt taccgcacca
     4141 atgaaaatac ctaagaacac ttccacattg tggatattgg caagagtggc ttgttccgct
     4201 aaaaatgccg cttgtgcagt ttcatctaaa tttgcaggca ttaatgcatt ggtatgaagt
     4261 ccgtagctgt taaaaccaac caataccgct gctaaaccga caaagctgtg tagaattgcc
     4321 actaattcag gcatttcggt catttctact tttagggctt ttttcactcc gatgaatcca
     4381 ccgattgcca ttgcgattaa gatccaaatc gtgccatgtg attgcgggcc gaaaatggtt
     4441 gccactaaag caatcgccat acccacaatc ccataccaac aacctgcttt ggcggtttcg
     4501 tgtttagaaa gaccggctaa gctcataata aagagaatag ctgcaataat gtacgcagca
     4561 gtcacaaatc caaaagacat agtttcgttt ctccttaacc ttttctaaac attgcaagca
     4621 tacgttgcgt tactttaaag ccaccaaaaa tattgatgct ggcaaccaaa atcgcaataa
     4681 atgccaaaat atcaataaag aaattaccgg tcggttgggc aatttgtaat accgcaccta
     4741 cgatgataat ccctgaaatc gcatttgtta ccgccattaa tggggtatgt agtgcgtggc
     4801 tgacgttcca aaccacataa taccctacca cacaagcgag aacgaatacg gtaaaatgcg
     4861 ataaaaacgc tgccggagcc acagatgcaa gccataggaa cagcacgcct gctgctgcca
     4921 tcacgccata tttcactcga gggtctgcag gtttttcctc ttttttgacc gcttgtggtg
     4981 cggctgcttg tttttgcggt tgggcagata cttgaatcgg cggagccggc caagttaatt
     5041 cgccgtcacg caccaccgtt acgccacgca acactacatc atcaaaatta atctcaattt
     5101 gaccatcttt atttggtgct aacagtttga gtaaattcac caagttagta ccataaagtt
     5161 gcgaagattg cgttggtaaa cgtgcaggaa aatcggtata gccaatcact ttcacttgat
     5221 aatcggtcac cacaatctcg ccggctttgg tgtattcaca gttaccaccg gttgcagcgg
     5281 ctaaatcaac aatcactgaa cccggtttca ttgaatcaac catctcttta gtgattaaac
     5341 gaggagcagg tttgcccgga atggcagcgg tggtaataat aatatccacc tctttggctt
     5401 gctcggcata taacgccatt gcacgacggt taaactcttc cgacatcact ttcgcatagc
     5461 cgtcacctga accaccttct tcttcaaaat caatttcgag gaaatcagcc cccattgatt
     5521 taacttgctc ttttacttca gggcgagagt caaaggcacg cacaatcgcc cccaggctat
     5581 ttgccgcccc gattgcagcc aagccagcaa caccggctcc aattaccaac acttttgccg
     5641 gtggtacttt acctgctgcg gtaatttgcc cagtaaagaa gctaccaaaa gcgtttgccg
     5701 cttcgatcac cgcacggtaa cccgaaatat ttgccatcga acttaaagca tcgagagctt
     5761 gggcacggga aatacgaggc acagcgtcca ttgccaacac attaattttc ttagactgca
     5821 atttttccat caattgtgga ttttgtgccg gccaaataaa gctgactaag gttacccctt
     5881 ctttgattaa ctcaatttca gcttctgtcg gcgcattcac tttaaaaata atgtctgcat
     5941 tccaaacttc ttgttgtgtg ccgatatttg cacctgcatt taaaaatgca ttatcttcaa
     6001 aactggcttt aaaacctgcg tcttgttcca caagtacgtc aaagccaagt tttttgatct
     6061 gctcaacggt ttttggcgtt gccgccactc gagtttcacc gtctaacagc tctcttggta
     6121 caccaataag cataaagact cctgtatggt tgttgtgatt aaaaacgctc taaatgtacc
     6181 actaaataaa acaaataggt aaaaaattat gcccaattaa accgattatt tcccattttt
     6241 tcaaatttta agcaggctaa atttttgcta cactattaac aggctaaatt taacaaaaaa
     6301 gaaacaaaac caatgcagat tctcaaccaa attcagatca ttcttgttga aacttcactc
     6361 ccggcaaata tcggttccgc agcccgagca atgaaaacga tgggcttaac ccatttgcga
     6421 ttagtcgccc caaaacaacc gcttgatgaa caagccgaag cccttgctgc tggggcaaaa
     6481 gatgtgttgg ataacgctca atgctacgcc actttcgatg aagccattgc cgattgccaa
     6541 ctggtgatcg gcaccagtgc cagacttcgc catttgcaaa ataccctgat tgaaccaaga
     6601 gactgtggtg aattagtggt aaatcgagcc aatcttggca aagtggcgat tgtatttgga
     6661 cgtgagcgag tggggctaac caacgaagaa ttattgaaat gccagtacca tttgaatttc
     6721 ccaaccaatc ctgattatgg ttctctgaat ttggcaatgg cagtacagct cgcaagttat
     6781 gaaatccgaa tggcttattt gcaaaattct ggcgaaaaac aaccgcttgc agcagataag
     6841 gattacccaa ccgctcaagc gttagagcat ttctttgctc acaccgaacg cttatatacc
     6901 gaattaggct ttatccgcaa tgattcagtg atgctaaaac tccgccgttt ataccaacgt
     6961 gccgaactgg aaactaatga gctgaattta ctgaggggaa tgctcacggc ggtggagaag
     7021 gcaaattgac aagcggttaa tttttagtag aaatttgcaa aatccctgac taaaacaaaa
     7081 acggtaagat tactcttacc gttttctcta aatcattatt ttaaattcaa cagtttattt
     7141 gaactttcaa taatactgcc cattgtagaa tcgcctttta attgttcttc gctcatcggt
     7201 tcgatatgcc aaatattttt cgcataatcc atcaccgagc ggtctgagga gaaatagccc
     7261 atattggcaa tattgatgat ggctgaacgt gtccaagctt tcttgttacg ataggcttga
     7321 gccacttttt cttgagcctc cacatagcta cgataatcgg cacaggcttg gtagtagtca
     7381 cctgaattta agatcaaatc ttgatagcga tacggatctt ccggtgagaa tttaccgttt
     7441 agaatttgtg aaattgcctc attcaattcg ctgtcttttt cgtagtagtg atacggtgag
     7501 taaccgttgc ggcgaagctc ttccacctgc tctacttcat taccgaaaat aaagatgttc
     7561 tctttaccaa cgtgatctaa aatttcgaca ttcgcaccgt ctaatgtacc aatggttaaa
     7621 gcaccattga gggcaaattt catattagag gttccggagg cctctgtacc cgctaaggaa
     7681 atttgctctg aaacatcagc tgccggaata atcatttgtg ctaatgaaac accgtagttc
     7741 ggaataaaca ccaccttaat taaatcatta atgcgagggt cattattcac cacatttgcc
     7801 acatcattaa tcaaacgaat cacttttttc gccgcataat aagcacttgc cgctttccct
     7861 gcgaagataa acacacgagg tgtccaatca gcagtcgggt ttttcaagat gcggttataa
     7921 agcgtgatga tatgcaacac gttcatttgc tggcgtttat acttgtgaat acgcttaact
     7981 tgtacatcaa aaatcgcttc aggattaagt ttaattccct gagtttcttc gacatatttg
     8041 gctaagcgac gtttgttttc tactttcacc gctgccacct gtgcttgcac atcagcatca
     8101 tcaacaaaca cgttgaattt ttccaattca cttaaatcag tcagccactt att
//
LOCUS       DANKBV010000102         8151 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.102, whole genome shotgun sequence.
ACCESSION   DANKBV010000102 DANKBV010000000
VERSION     DANKBV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.102"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(131..1873)
                     /locus_tag="PWY43_001839"
     CDS             complement(131..1873)
                     /locus_tag="PWY43_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HDL2136701.1"
                     /translation="MSIAIGGLSKATDIGAVALGTSSYASGFNSFAAMRQSAARADYA
                     AAIGSSSWANATASFAIGASATALGERSFAIGSGVPQTLDQNTGEARRSIYDGNNNTL
                     AAGKDSFSIGTKAKTNGDSAFAIGTNAHSGSFKVEASNIGELGDITVADKDKVANNAF
                     ALGTNTRALSNDTVAFSTNATAQGDGSIAFGVNTNTTGTGAIAFGNASKAWKDNAISI
                     GWNATSHAEKVITIGTNSTVTKEGGMAIGDTAKSTGNNSIAVGYTSNVSNSNATAIGT
                     RANVTHEGGTAIGFEAESIANYATAIAYNSTASGESSIAAGVNSTVSGIRAAAVGYNN
                     TVSGNDTFVLGSNVTVNTVGSVILGSDSAQAKATYENTATVGKLTYSGFAGNDNVAEG
                     DYVSVGNATNARQIKFVAPGNISSTSTDAINGSQLYATNKMLDNVTNSVVNNFGGNSQ
                     VDANGNITFTNIGETGKDTIHDAIKAAKTQVKQGSNVNVSKATVDGTDVYTVNAYNTT
                     VSAVTTNGKNYLNVADNFNTGTNTTNYAVSLTDDAIRDFTKDNDTVTGVVDDGKDYKL
                     KKFHLQKMKIALTK"
     gene            complement(2089..2346)
                     /locus_tag="PWY43_001840"
     CDS             complement(2089..2346)
                     /locus_tag="PWY43_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HDL2136702.1"
                     /translation="MNKIFKVIFNRTTQKMEVVSELAKSQGKAASSTDDRSSISPLVG
                     GLKGSFLVGGTALLMISAMPAEAVVSQVIMLDILQAILLTK"
     gene            complement(3091..3833)
                     /locus_tag="PWY43_001841"
                     /pseudo
     CDS             complement(3091..3833)
                     /locus_tag="PWY43_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4004..4342)
                     /locus_tag="PWY43_001842"
     CDS             complement(4004..4342)
                     /locus_tag="PWY43_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2136703.1"
                     /translation="MKLITTIIQPSKLDQVQDALSEIGIQGMTITEVKGFGRQYGHTE
                     QELSQMSRIDFRPKIKVEILVSNYQTDEVIDAILNASYSGQVGDGKIYVANIEQVIRT
                     RTGETDDEAV"
     gene            4600..6498
                     /gene="dnaK"
                     /locus_tag="PWY43_001843"
     CDS             4600..6498
                     /gene="dnaK"
                     /locus_tag="PWY43_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL2136704.1"
                     /translation="MGKIIGIDLGTTNSCVAVMDGDKPRVIENAEGARTTPSIIAYTD
                     KETLVGQPAKRQAITNPKNTLFAIKRLIGRRFTDAEVQRDIEIMPFEISKADNGDAWV
                     TVKGEKLAPPQISAEILKKMKKTAEDFLGEPVTEAVITVPAYFNDAQRQATKDAGRIA
                     GLDVKRIINEPTAAALAYGLDSKKENQTIAVYDLGGGTFDISIIEIDNFDGEQTFEVR
                     ATNGDTHLGGEDFDNRVINYLVEEFKKEQAVDLRNDPMAMQRVKEAAEKAKIELSSAQ
                     ETEVNLPYITADASGPKHLNIKVTRAKLESLVEDLVNRSLEPLKTALSDAGLSVSDIN
                     DVILVGGQTRMPLVQKKVADFFGKEPRKDVNPDEAVAIGAAVQGGVLSGSVTDVLLLD
                     VTPLSLGIETMGGVMTTLIEKNTTIPTKKSQVFSTAEDNQSAVTIHVLQGERKRAADN
                     KSLGQFNLEGINPAPRGMPQIEVTFDIDANGIINVSAKDKNTGKEQQIKIQASSGLSD
                     EEVEQMVRDAEANAEADKKFEELVQTRNQADGIAHATRKQITEAGDALNADDKAKIDA
                     AIAELETAAKGEDKAEIEAKIEALIKASEPLMQAAQAKTQGGEQPQQSQKDDGVVDAE
                     FEEVKDNK"
     gene            6640..6942
                     /locus_tag="PWY43_001844"
     CDS             6640..6942
                     /locus_tag="PWY43_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136705.1"
                     /translation="MLKLYPTTENVQPPFYKIYDHKSGETVIATEMENSNTPILMDDF
                     VWFEQIPTTEALLVDTWSVIENRAKQGLLKRAYTKDFEKHLADGKVEIYIAVNPHC"
     gene            7108..>8151
                     /gene="dnaJ"
                     /locus_tag="PWY43_001845"
     CDS             7108..>8151
                     /gene="dnaJ"
                     /locus_tag="PWY43_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2136706.1"
                     /translation="MAKKDYYEVLGLSKGASEKDIKRAYKRLAAKHHPDKNQGSKESE
                     EKFKEITEAYDVLTDSEKKAMYDQYGHAAFEQGGAGAGGFGGFGGGGFGGFEDIFSEM
                     FGSGFGGGGRRQRVVRGDDLRYDIEITLEEAVKGCKKDIRIQTLAECDTCHGTGAEAG
                     SKVETCSHCHGSGRVRRQQGFFVTEAVCPSCHGTGKRIEKPCRSCHGDGRVQKAKNLS
                     VTIPAGVDTGNQLRLSREGAAGENGAPAGDLYVVIHVKEHDIFERDGSNLYCEVPISF
                     TLAALGGEIEVPTLDGKLKLKIPAETQTGKLFRVRGKGVTSPRGGYAGDLICKVVVET
                     PVNLTEEQKALLRQ"
BASE COUNT         2655 a         1662 c         1557 g         2277 t
ORIGIN      
        1 tcaaccgtat aatccataat atctggatta gttgtattag ctgccccgtt tacattaatt
       61 ctattagaac cagctttcac agttgcagaa gtatcttttg tgaactcatt aattgcctta
      121 tcacttaagc ttactttgta agtgcgattt tcatcttctg gagatggaat tttttcaact
      181 tataatcttt accatcatca actacaccag taacagtatc attatcttta gtgaaatcac
      241 gaatcgcatc gtctgttaaa ctcaccgcat aatttgtagt atttgttcca gtattaaaat
      301 tatcagctac atttaagtag tttttaccat tcgttgtaac agcacttaca gtagtgttat
      361 atgcattgac agtatataca tctgtaccat caactgttgc tttagaaaca tttacattag
      421 aaccctgttt aacttgggtt ttagctgctt tgattgcatc atgaatagta tctttacctg
      481 tttcaccaat atttgtaaat gtgatattac catttgcatc aacttgtgaa ttaccgccaa
      541 aattatttac aactgaatta gtcacattgt ctaacatttt attcgttgca tataattgag
      601 agccgttaat tgcatctgtt gaagtgcttg agatattacc tggtgctaca aatttaatct
      661 ggcgagcatt tgttgcatta cctacactta cgtaatcacc ctcagcaaca ttatcgttac
      721 cagcgaaacc actataagtc aatttaccta cagttgctgt attttcataa gtcgcttttg
      781 cttgtgcgga atctgagcct aaaattacag aaccaacagt attaaccgta acattagaac
      841 ctaatacaaa ggtgtcatta cccgatacag tattgttata acctaccgct gctgcacgaa
      901 taccggaaac agtgctgttt acacctgccg caatagagct ttcaccactt gctgttgagt
      961 tataagcaat cgccgttgca tagttagcta ttgactcagc ttcaaaacca atcgcagtac
     1021 caccttcgtg ggttacattc gctcgagtac caattgctgt tgcattcgag ttgctaacat
     1081 ttgaagtata acctaccgca atagaattat taccagtcga ttttgctgta tcaccaattg
     1141 ccatcccacc ttctttagtt acagtagaat tggtaccaat agtaattact ttttctgcat
     1201 gagaagttgc attccaacca atactaatag cattatcttt ccaagcctta gatgcattac
     1261 caaatgcaat tgcacccgta ccagttgtat tagtatttac accaaaagca atagaaccat
     1321 ctccctgtgc agttgcatta gttgagaaag ctacagtatc gtttgataat gcacgagtat
     1381 ttgtacctaa agcaaaagcg ttatttgcaa ccttatcttt atctgcaaca gtaatgtcac
     1441 ctaattcacc aatattacta gcctcaactt taaagctacc agaatgtgca ttggtaccaa
     1501 ttgcaaaagc actatcacca ttagtttttg ctttagtacc aatagaaaat gaatccttac
     1561 cagcagctaa agtattatta ttaccatcat aaatacttcg tcttgcttct ccagtatttt
     1621 gatcaagagt ctgaggtaca cctgaaccaa tcgcaaaaga acgctcaccc aatgcagttg
     1681 ccgatgcacc aattgcaaaa cttgcagttg catttgccca agaagaagaa cctattgctg
     1741 ccgcataatc agctcttgca gcagattggc gcattgctgc gaatgagttg aacccagaag
     1801 cataagaaga tgtacctaaa gctactgctc cgatatctgt cgctttagat aaaccaccaa
     1861 ttgcaataga caaaccacct gtcgatttag aatagtcacc gatagcaata ccattattgt
     1921 aaccagaagt aacttgacta ctctgaataa ctgtattacg acctatgcca ataccttcta
     1981 tatgtagcgt tccattataa agattcccag tggtacttct gtcaatattt aaaggatcgt
     2041 tataagaaag actacctgga ttataatagt tataagcgta tgtatcccct attttgttaa
     2101 tagaatcgcc tgtagtatat ccagcattat tacctgagaa acaactgcct ccgccggcat
     2161 tgccgaaatc attaataacg cggttccacc tactaaaaaa gaacctttta gcccacccac
     2221 taaagggcta atactactac gatcatcagt ggaggatgcc gcttttcctt gcgatttagc
     2281 aagttcagac acaacttcca ttttttgcgt cgttcggtta aaaataacct taaatatttt
     2341 gttcataatt ttataccttt agatatataa aaaagcttat ttctgacgtt agaattaacc
     2401 ttagaaataa gcttacttcg taaataattc ggcaattaca acccaccgaa taaacaactt
     2461 tactgctctt tataagagga cagtcctctc gataatagta agattttcac ttcaaaacct
     2521 caacaatctt ttaacatctt tacaattctc ttattatcaa gataataaaa ttaacaaaac
     2581 cccttataaa tcaaacaaat acacagaaaa tatacttaat ttacaaataa ataccaaacg
     2641 gaaaattatt aacattctac ctatttttaa ttaaccaata tgcaacaaag ccatattaat
     2701 aataaggtta tcactggttt ttatccctta ttttttatga aaaatcttat aatattgaat
     2761 atttactcca gttttaatcg agaatcatgt tcttttttat tttgttattc tctttaggta
     2821 taaaaaaacc cggtaaatac cgggctttta tttcaatgag ttatccaact tgctagagcg
     2881 attaccgcaa taaaaataaa taaccaaata agttgtaatt ttacgctttt gcggttgatc
     2941 tcctggttgt gtaatcggcg ctgttctaac ttttgccgat atatcactaa atcggcttct
     3001 ttaaacgaga agccacaatg tatgcaatgt tctaaactgt cgctaatacg ctttcgacac
     3061 tctggacaac ggtgtaatgc cataattacc tcaatttatt actttgcata atcggctatc
     3121 cgtacattgt tcaatatgaa catgatagta ttgatgagta tcacgattga aaaacaagaa
     3181 atattgatta cctcttattt ttgttcccat aatgtagccg ttatcactgt tggtggataa
     3241 tatgtagtat ttttgattct ctatttcgat gtaatcatag gtagttctta gtccggcacg
     3301 aaataaccaa tatgcaatga gagcaaactg aaatagagaa aaataaagat gttaaacaga
     3361 gcaaaacttc ctcctaatag catctttcta aagtctagat caaacttggt attattccat
     3421 ctcttacgga agaagaatac actcacgcca cttacaaaaa tatagataaa tgagatataa
     3481 ataggcacta taccggtaaa taagtaaaaa gtgagaataa ccggtactgt aaatacagaa
     3541 accaacacaa tcacccttaa ccaacctaga tttcggaaat aatgcagctg aaaaacttta
     3601 tttagtaagc tataacctag cgcataacac aagaataaga taactgaagt accgaataca
     3661 taagccaaac ttcttgccat actggcattg ccgatttcaa catgccacca agggtagcca
     3721 tgaaatatgg cttgtcccca accgtaacag taagcggtag caaaactagt taatgtaaaa
     3781 aatgcaagag aaaatgaaga attgataatt ttattaatgt gtttatttag cataacaaag
     3841 aatgtctatt tccattaaaa cctaactatt tttgattact ataataaccc gtattacaca
     3901 aaaaacaaac atttaattca caccaaatta gaaaatttgc tttagtaagg ttgaaaatat
     3961 aactctaaaa cacgataata aaaagcttct ctgctaacaa tggctagact gcttcatcgt
     4021 cagtttcgcc ggtacgggtg cggataactt gttcaatatt cgctacataa attttgccat
     4081 cacccacttg cccagaatag gatgcattga gaatggcatc gatcacttca tcggtttggt
     4141 aattagaaac caatatctcc accttaattt taggacgaaa gtcaatgcgg ctcatttggc
     4201 taagctcttg ttcggtatgc ccatattggc gtccaaagcc ttttacttcg gtaatagtca
     4261 ttccttgaat accgatttct gaaagtgcat cttgcacttg gtctaatttt gaaggttgga
     4321 taatggtagt aatcagtttc atattatatc cttctctgtt tgagttgccg taagtataaa
     4381 aagctattga ggggtatgca aggtaggagg tattttctcc gtgaaatatt ctgccaaatc
     4441 tcccccacat acaagcggtt actttttcca aaaattttgc aattttttta tttttgccct
     4501 tgaacagtgc attttcgccc ttatttatgg tttcgtctga acgggaaatg caatcccgtt
     4561 tcttattcaa actgaattta ttattagagg aatcataaaa tgggtaaaat cattggtatt
     4621 gacttaggta caacaaactc ttgtgttgcg gtaatggacg gcgacaaacc tcgtgtaatt
     4681 gaaaatgcgg aaggggcaag aacaacgcca tctatcatcg cttacaccga taaagaaacc
     4741 ttagttggtc agccggcaaa acgtcaggca attactaacc cgaaaaatac tttatttgca
     4801 atcaagcgtt taatcggtcg ccgctttact gatgcagaag tacaacgtga tattgaaatt
     4861 atgccgtttg aaatcagcaa agcagacaac ggcgatgcgt gggtaacggt aaaaggcgaa
     4921 aaattggcac caccacaaat ttctgctgaa atcttgaaaa aaatgaagaa aaccgcagaa
     4981 gatttcttag gtgagccggt aaccgaagca gttattactg tgcctgcata ctttaacgat
     5041 gcacaacgcc aagccactaa agatgcgggt cgtattgcag gtttagatgt taaacgtatc
     5101 attaacgaac caacagcggc ggcacttgcc tacggtttag attctaagaa agagaaccaa
     5161 actatcgccg tttacgactt aggtggtggt acattcgata tttcaattat cgaaatcgac
     5221 aactttgacg gcgagcaaac ctttgaagtg cgtgcaacca atggtgacac ccacttaggt
     5281 ggtgaagatt tcgacaaccg agtcattaac tacctagtag aagaattcaa aaaagaacaa
     5341 gcggttgatt tacgcaacga cccaatggca atgcaacgtg tgaaagaagc ggcggaaaaa
     5401 gcgaaaattg agctttcttc ggcacaagaa actgaagtaa atcttccgta catcacagca
     5461 gatgcaagtg gtccgaaaca cttaaacatc aaagtgactc gtgcgaaatt agaatcttta
     5521 gttgaagatt tagtaaaccg ttcattagag ccattaaaaa cggctttatc tgacgctggt
     5581 ttatcagtta gcgacatcaa cgatgtaatc ttagtgggcg gtcaaacccg tatgccatta
     5641 gtacagaaaa aagtggcaga cttcttcggc aaagaaccac gcaaagacgt gaacccagac
     5701 gaagcggtag caatcggtgc agcagtgcaa ggtggcgtat taagcggctc ggtaacagac
     5761 gtattgttat tagacgtaac cccactttca ttgggtatcg aaacaatggg cggcgtgatg
     5821 acaaccttaa ttgagaaaaa cactacgatc ccaaccaaaa aatcgcaagt gttctcaaca
     5881 gcggaagaca accaatctgc ggtaaccatc cacgtgttac aaggtgagcg taaacgtgca
     5941 gcggataaca aatcgttagg tcaattcaat cttgaaggca tcaacccagc tccacgcggt
     6001 atgccacaaa ttgaagtaac tttcgatatt gacgcgaacg gtatcatcaa cgtgtctgcg
     6061 aaagacaaaa acactggcaa agaacaacaa atcaaaatcc aagcatcttc aggcttaagc
     6121 gatgaagaag tagaacaaat ggtgcgtgat gcggaagcga acgctgaagc agacaaaaaa
     6181 tttgaagagt tagttcaaac ccgcaaccaa gcagatggta ttgctcacgc aacccgtaag
     6241 caaattactg aagcaggcga tgcgttaaat gcagacgaca aagcgaaaat cgacgcagcc
     6301 attgcagaat tagaaaccgc tgcaaaaggc gaagacaaag cggagatcga agcaaaaatc
     6361 gaagcgttaa tcaaagcttc agaaccgtta atgcaagcag cccaagcaaa aacccaaggc
     6421 ggcgagcaac cacaacaatc ccaaaaagat gacggcgtgg tcgatgcgga atttgaagaa
     6481 gtgaaagata ataaataatc ttgcgtaact tgtaggggcg aaatatcttt cgcccctttt
     6541 gtgtagatat ataccaccta gcacgagccg tgctaggtta cacataattc agcccctttg
     6601 gggctgtgca agcggtgctt ttcaaaggaa attttgcaaa tgctaaaact ctaccctaca
     6661 acggaaaatg tacagccacc gttttataaa atttacgatc ataaaagtgg tgaaacggtc
     6721 attgctacag aaatggaaaa cagcaatacc ccgattttaa tggacgattt tgtttggttt
     6781 gagcaaattc caaccaccga ggctcttttg gtagatactt ggtctgtgat tgaaaaccgt
     6841 gctaaacaag gcttgctcaa acgagcttac accaaagatt ttgaaaaaca tttagcagac
     6901 ggcaaagtag aaatttatat tgcggtgaat ccgcattgtt aggctttgat agcgcaagcg
     6961 tccacgcttg tgcttaacca tcaaatttgg tacaagcgtg gacgcttgca ccatcttgtc
     7021 tagaacctat tttactataa aacgctctcc cacaaggggc gagggcaagc ggtcaaaaac
     7081 agagaaaaat ttacaaacgg aacaacaatg gcaaagaaag attattacga agtccttgga
     7141 ctgtctaaag gggcaagcga gaaagacatc aaacgagcat ataaacgttt ggcggcaaag
     7201 catcacccgg ataaaaacca aggcagtaaa gagtcggaag agaaatttaa agagattacc
     7261 gaagcctatg acgtgctgac cgacagcgag aaaaaagcga tgtacgacca atacggtcac
     7321 gccgcctttg aacaaggcgg tgccggtgca ggcggattcg gtggctttgg cggcggtggt
     7381 ttcggtggtt ttgaagacat cttcagcgaa atgtttggca gtggctttgg tggcggtgga
     7441 cgtcgccaac gtgtggtacg tggcgatgat ttacgttacg acatcgagat cacccttgaa
     7501 gaagctgtga aaggctgtaa aaaagatatt cgcattcaaa ccttagctga atgtgatact
     7561 tgtcatggta caggggcgga agcagggagc aaagtggaaa cctgttcgca ttgtcacggc
     7621 tccggcagag ttcgccgtca gcaaggtttc tttgtgactg aggcagtttg tccaagttgt
     7681 cacggtacag gtaaacgcat tgaaaaaccg tgccgttctt gccacggcga tggacgtgta
     7741 cagaaagcga aaaacctctc tgttaccatt ccggcaggtg tggataccgg taatcaactt
     7801 cgcttatcga gagagggagc tgcaggcgaa aatggcgcac cggcaggcga tttatatgtg
     7861 gtgattcacg ttaaagaaca cgatattttt gagcgtgacg gttcgaattt atactgtgaa
     7921 gtgccgatta gctttactct tgcagcttta ggtggtgaaa ttgaagtgcc aactttagac
     7981 ggcaaattaa aactcaaaat tccggcagaa actcaaaccg gtaaactatt ccgtgtacga
     8041 ggcaaagggg taacaagccc tcgtggtggc tacgcagggg atttaatctg caaagtggtg
     8101 gtcgaaacgc ctgtgaattt aaccgaagaa cagaaagcac tattacgaca a
//
LOCUS       DANKBV010000103         8122 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.103, whole genome shotgun sequence.
ACCESSION   DANKBV010000103 DANKBV010000000
VERSION     DANKBV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.103"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..436
                     /locus_tag="PWY43_001846"
     CDS             <1..436
                     /locus_tag="PWY43_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase"
                     /protein_id="HDL2136707.1"
                     /translation="IEGIENRLISFANCCLPIPGDEIIAHVSSGKGLVIHNACCGNVK
                     DYATEPSNYLPVEWEIAKEKAIQFETEIVLDIINEPGVLAGITSTLAKLNSNIGSINS
                     EAREGNIYQVRVQISVENKEHSELILRKLLSVKGVVKAARKQ"
     gene            648..1595
                     /locus_tag="PWY43_001847"
     CDS             648..1595
                     /locus_tag="PWY43_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL2136708.1"
                     /translation="MPTKPHKEYHELVELLISRGMLINDKSYAEKKLSQVGYYRLSGF
                     WHISRKPNQDGGLSENFLENVQFEEVYKLYIFDKKLRLLLLDIIERLEVNIRNIMAHE
                     LGRENPLAYEDYSLINQKFLKFQWKSNYAYANWLETTANKIKRSKDDSIEWHRKNEKE
                     IPFWVITETWDFGALSKFYSLLNGQHQRKIAKKFNVDENTLKNWLNEINIVRNLSAHH
                     SRIWNRKFSDIAIPKLDDPRWSKFKKMQQYFSNLSLDDKAKERIFGRVAILWFLVSQT
                     STNYHWLDKFEDLLNDFPNLPNTKVELMGFNREILEKIK"
     gene            complement(1659..2621)
                     /gene="hemH"
                     /locus_tag="PWY43_001848"
     CDS             complement(1659..2621)
                     /gene="hemH"
                     /locus_tag="PWY43_001848"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDL2136709.1"
                     /translation="MNQPQQIGILLANLGTPDEPTPSAVKRYLKQFLSDPRVIDLPKF
                     KWQAILNCIILPRRSPKVAKLYQSVWSEEGSPLLAISRQQQRAVQRYFDAKGKNVVVE
                     LGMSYGNPSIESATDNLIKQGVERIIVLPLYPQYSSTTTASVFDAFANGLKAQRKIVP
                     FDFIHSYHNDPLYIQALANSIDVKPDELLLFSFHGIPKRYETEGDFYAKHCQETAELV
                     AEKLGLPREKWYLSFQSRFGDEEWLQPYTDETLESFPQKGVKKVAVVCPGFSADCLET
                     LEEIAEENRENFEHTGGESYRYIPALNASGDHIKALVKLIEAKI"
     gene            2858..2992
                     /gene="rpmH"
                     /locus_tag="PWY43_001849"
     CDS             2858..2992
                     /gene="rpmH"
                     /locus_tag="PWY43_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL2136710.1"
                     /translation="MKRTFQPSVLKRARTHGFRARMATKNGRQVLARRRAKGRKSLSA
                     "
     gene            3028..3402
                     /gene="rnpA"
                     /locus_tag="PWY43_001850"
     CDS             3028..3402
                     /gene="rnpA"
                     /locus_tag="PWY43_001850"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL2136711.1"
                     /translation="MKKLNFSRELRLLAPVQFKAVFEEPLRASTPQMTLLARENNLEN
                     PRLGLTVAKKHLKRAHDRNRIKRIVRESFRLKQHELPNFDFVFVAKGGIGKLDNATLF
                     ETLEKLWSRHIRLSKKQAQQNP"
     gene            3351..3674
                     /gene="yidD"
                     /locus_tag="PWY43_001851"
     CDS             3351..3674
                     /gene="yidD"
                     /locus_tag="PWY43_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL2136712.1"
                     /translation="MVKTHSPEQKAGTTESINQPPRLSLLAKILLLPIYFYRYAISPL
                     IGPRCRFYPTCSTYAVEAIKIHGAIKGGYLAFKRILRCNPLSEGGEDPVPLCCKGNCK
                     HNDKK"
     gene            complement(3779..4120)
                     /gene="erpA"
                     /locus_tag="PWY43_001852"
     CDS             complement(3779..4120)
                     /gene="erpA"
                     /locus_tag="PWY43_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDL2136713.1"
                     /translation="MSDIQIPLIFTDAAARKVKSLIEGEENPNLRLRVYITGGGCSGF
                     QYGFTFDDQVNEGDLTIENQSVGLVVDPMSLQYLIGGTVDYTEGLDGSRFVVNNPNAS
                     STCGCGSSFSI"
     gene            4269..4604
                     /locus_tag="PWY43_001853"
                     /pseudo
     CDS             4269..4604
                     /locus_tag="PWY43_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determination protein"
     gene            4920..5768
                     /locus_tag="PWY43_001854"
     CDS             4920..5768
                     /locus_tag="PWY43_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2136714.1"
                     /translation="MDAKVEDRRITDLKARISALNLISMVNFTDRISVNWGDYSQIQV
                     EMLLFKKAHRNSSYAYYHLLSGVDLPLVSQDTIHQFFDKHQGKEFLTIVSKESFERNK
                     IYDRVRIHYWAPHISERTFSNKLIGKFFQKFIRSSELYMKRICSADKFKTFGTALGYA
                     SQWVSITDELVTILLDEELWIRDVFSHSLFCDELFIPTIINKYGWESRIYYSKGMVND
                     PEEFQGNLRYINWWDGSPYTWTDSSEDFSSLEKAINRGHFFARKFDIQTYPAMIDFIK
                     ARTQFS"
     gene            5995..7098
                     /gene="glf"
                     /locus_tag="PWY43_001855"
     CDS             5995..7098
                     /gene="glf"
                     /locus_tag="PWY43_001855"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HDL2136715.1"
                     /translation="MRKYDYLIVGVGLFGTIFAYEATKRGKKCLVIEKRDHIGGNCYT
                     QNIEGINVHKYGAHIFHTSNKLVWDYIQQFAEFNRFTNSPVARYKDELYSLPFNMLTF
                     NKMWGVIKPEEAEAKIKEQIAQEHITEPKNLEEQAISLVGRDIYEKLIKGYTEKQWGR
                     KCTELPAFIIKRLPVRYTYDNNYFYDTYQGIPIGGYTGIFERMLDGIEVKLGVDFFLE
                     REYYENLADKIVFTGMIDEYFGYRFGRLEYRSLRFDNELLDIPNFQGNAVVNYTEAEV
                     PYTRIIEYKHFEYGIQPKTVMTREYSKEYEQGDEPYYPINDARNNELYAKYKVLADEK
                     SNVIFGGRLAQYKYFDMHNIIAEALACVDKHFK"
     gene            7501..>8122
                     /locus_tag="PWY43_001856"
     CDS             7501..>8122
                     /locus_tag="PWY43_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2136716.1"
                     /translation="MLFNLKFWQKPAKIITDSDSFRFQLFNDPYLGYVKADNIKHETL
                     FIDSTNISKDKLNSIIEDNIKNSREIIFSPFLVGYDFSQSSIYNLFDSRINVFHLENK
                     LLSKLNRFYKAIIDYTIVVLSSVFWLPLLCLIAIWIKIEDPKGDVFFMQRRLGLNGKE
                     FLCYKFRSMYSDQSFMADWLEMNPEEKAYYNIYHKYMNDPRITKVGS"
BASE COUNT         2650 a         1277 c         1522 g         2673 t
ORIGIN      
        1 aattgaaggg attgagaacc gccttatcag ttttgcaaac tgttgcttac ctataccggg
       61 tgatgaaatt attgctcacg tcagttcagg taaaggattg gtaatccaca atgcttgttg
      121 tggtaatgtg aaagattacg caacagagcc gagtaattat ttacctgtgg aatgggaaat
      181 tgccaaagaa aaagcgattc aatttgaaac ggaaatcgta ctggatatta tcaatgaacc
      241 aggggtactc gcggggatca catcaacgct tgccaaatta aatagcaaca ttggcagcat
      301 taatagcgag gcaagagaag gaaatatcta tcaagtgaga gtacaaattt ctgtggaaaa
      361 taaagagcat tctgagctga ttctccgtaa gctattaagt gtaaaaggtg ttgtaaaagc
      421 ggcccgtaag cagtaacaag cggtaaaatt ttatcagttt tttgcaatta tagtccttaa
      481 aagtacagta ttgctattat taattgatga aaccttttga ctttaatcag ctaaaatgta
      541 taatcccttg caatcgtggc gtagcccctg tccctgaggt ttactaaatg taatcgactt
      601 agagtgtcaa gctacgtcct tgcctttctt aaaatgcctt caattttatg cctacaaaac
      661 cacataaaga gtatcacgaa ttagtagaac tactcatatc tagaggaatg ctcataaatg
      721 ataaaagcta tgcagagaaa aaattatctc aggtagggta ttatcgttta tctggatttt
      781 ggcatatatc tagaaagcct aatcaagatg gtgggcttag cgaaaatttt ctcgaaaatg
      841 tacaatttga agaagtttat aagctatata tttttgataa aaaactaagg cttttattat
      901 tggatattat tgaacgttta gaagttaata ttcgtaatat tatggctcat gaattggggc
      961 gtgaaaatcc tttggcttat gaagattatt ctctgattaa tcaaaagttc ttaaaatttc
     1021 aatggaaatc taattatgct tatgcaaatt ggctagaaac tacagctaat aaaattaagc
     1081 gtagtaaaga tgattctatt gaatggcata gaaaaaatga gaaggaaatc cctttttggg
     1141 taattactga aacctgggat tttggtgcat tatctaagtt ttactcattg ttaaatgggc
     1201 aacaccaaag gaaaattgct aagaaattta atgtagatga gaatacgtta aagaattggt
     1261 tgaatgaaat aaacattgta agaaaccttt ctgctcacca ttctcgtatt tggaacagaa
     1321 aatttagcga tattgctatt cctaagctag atgatcctcg ttggtcgaaa tttaaaaaaa
     1381 tgcagcagta tttttcaaac ttatcgctgg atgacaaagc aaaggagaga attttcggaa
     1441 gggtagctat tctgtggttt ttagttagtc aaaccagcac taattaccat tggttagata
     1501 agtttgaaga tttattaaat gacttcccta atttaccgaa tacaaaagta gaactgatgg
     1561 gatttaacag agaaatatta gagaaaatta aataaaataa gtacattcct atagggtgta
     1621 gataaaaatg ggcggattta ttatccgccc aaattatatt atattttcgc ttcgattaat
     1681 tttactaatg ccttgatatg atcgccactc gcatttaacg ctggaatgta gcgataagat
     1741 tctccgccgg tgtgttcaaa gttttcacgg ttttcttctg caatttcttc cagggtctct
     1801 aagcaatcgg cggagaagcc cgggcaaaca acagccactt tttttacccc tttttgtgga
     1861 aaactttcaa gcgtttcatc ggtataaggc tgtagccact cttcatcacc aaaacgggac
     1921 tggaaagaca aataccattt ttctcttggc agacctagct tttcagccac taattctgct
     1981 gtttcttggc aatgcttggc gtaaaaatca ccttccgttt cataacgttt aggaatgccg
     2041 tgaaatgaaa acagtaataa ctcgtcaggt ttaacatcaa tagagttagc taatgcctga
     2101 atataaagcg gatcattatg ataactgtga ataaaatcaa acggcacgat tttacgttgt
     2161 gcttttaagc cgttggcgaa agcatcaaac accgaagcgg tagtagtgct actgtattgc
     2221 ggataaagcg gtagcacaat aattcgttca accccctgct tgatgaggtt atcggtcgca
     2281 ctttcaatac ttggattgcc gtaagacatt cccaattcca ccaccacatt tttgcctttg
     2341 gcatcaaaat agcgttgtac cgctcgttgt tgttgccgag aaatcgccaa taacggtgag
     2401 ccttcttcgc tccatactga ttgatataat tttgccactt ttggcgaacg tcttggcaaa
     2461 atgatgcagt ttaggattgc ttgccattta aattttggta gatcgatcac acgaggatcg
     2521 cttaaaaatt gctttaaata acgcttcacc gcacttggcg tgggttcatc tggcgtacct
     2581 aaattagcca gcaaaatacc tatttgttgg ggttgattca ttgttctatt tctcatcaat
     2641 agttaatatt ttttcgatta tgccacaaac caatataaat ttctgcgatt ataacaagcg
     2701 gtcgtatttt tcaaaaattt tgcaaacaac ccgagttgtg cttggattgt ttcttgcttt
     2761 ttattttaat tgcctgtaga attgcaactc ttttttatgg tccttgtttg gcagaaacca
     2821 ttttatgcca actttttaaa ttttaggtag ataagcaatg aaacgtacat ttcaaccatc
     2881 agtattaaaa cgtgctcgta ctcacggttt ccgtgctcgt atggctacta aaaacggtcg
     2941 tcaagtgtta gcgcgtcgtc gtgctaaagg tcgtaaatct ttatctgcat aattgcagaa
     3001 ccagccaaaa cacctttctg attaagtgtg aagaagctaa atttctctcg ggagctacgt
     3061 ttgttagctc ccgttcaatt taaggcggtg tttgaagagc cacttcgagc cagcacaccc
     3121 caaatgactc ttcttgctag agagaataat ctagaaaatc ctcgtctagg cttaactgtt
     3181 gccaaaaaac atttaaaacg ggctcacgac cgtaatcgca ttaagcggat agtgcgtgaa
     3241 agttttcgcc tcaagcaaca cgaattgccg aatttcgatt ttgtatttgt ggcaaaaggc
     3301 ggcattggca aattagacaa cgcaacgctg tttgaaacat tggaaaaact atggtcaaga
     3361 cacattcgcc tgagcaaaaa gcaggcacaa cagaatccat aaatcagcca cctcgtctca
     3421 gcctattagc caaaattcta ttgctaccga tttatttcta tcgctatgct atcagcccat
     3481 tgattggccc acgttgccga ttttacccaa cctgctccac ctatgcggtc gaagcgatta
     3541 aaatccacgg agcaattaaa ggtgggtatt tagcgtttaa gcgtattctg cgttgcaacc
     3601 ctttgagtga gggaggcgaa gatcctgtcc cactttgttg taaaggcaac tgcaaacata
     3661 atgataaaaa atagcctaaa ctcaataagt taggctattt tgtttgatac aaacggtcat
     3721 ttttagtaaa aaatttactt gtgagactga tattgaataa ggcaataatt cgagtgaatt
     3781 aaatgctaaa ggaagaacca caaccacagg ttgagcttgc atttggatta ttcacgacaa
     3841 aacgtgagcc gtccagccct tcggtataat ccaccgtgcc accaattaaa tattgtaggc
     3901 tcatcgggtc aaccaccaag ccaacgcttt gattctcaat ggtaaggtct ccctcattga
     3961 cttgatcatc aaaggtgaac ccatactgga atccactaca gcccccaccc gtaatataca
     4021 cacgcaagcg aagattcggg ttctcttcgc cctcaattaa gctttttact ttacgagccg
     4081 ccgcatcagt aaagattaaa ggaatttgaa tatcgctcat tttgttttag atcaaaattt
     4141 tactgtgatt ggatattatc taataattac ataggaagtg caatcttaaa aaagagtaaa
     4201 tgaatgcagt ggaaataaga aaattatgta taatgctgta cagaaacatg caaattttag
     4261 aggaaactat ggcagattca gtaaaaagtc atgagcaaag ctcggatgag attgatttaa
     4321 ttgagatttt tcgtgctcta tgggataaaa aatggtggat tgttataaca acattcatta
     4381 ccacattact tgctggtatt tatgcattta ctgcaaaaga gcaatggacg tctaaggcaa
     4441 cagttattgc ccctaaatta acagagttag gtgagtattt aaatgttcgc agagaatata
     4501 gtcggatttt gcgtgtagat cctgttgatc caaatgcatt gtctgcgagt ttgttcaaca
     4561 attttaaccg attagcacaa tctcaagata ctaaaaatga attttttatc actttactta
     4621 aataagcaag attatctttt attacttcta acagagatat ttaatttgat taaaaaatag
     4681 ttgtcataaa atatgaagga tacttcaatt catttatatc tgctgggatt agagttatct
     4741 tgtatgcttt agcttctgaa aaggctaatt agagctttgt gagaaaatct agttgtattc
     4801 atcaaaaaca ggtttattat gtctaaaaag catgcttatc ttataattgc acatgaaaaa
     4861 tttgagcaat tactcttttt aattgagtta cttaatcatc ctagaaatga tatttattta
     4921 ttgatgctaa agtagaggat cgccgtatta ctgatttgaa agcaagaatc agtgctctga
     4981 atttgatatc aatggtaaat tttactgata gaatttctgt aaactggggc gattattctc
     5041 aaatacaggt tgaaatgctt ctctttaaga aagctcatag aaatagcagc tatgcttact
     5101 atcatttgct atctggtgtt gatttacctc tagtcagcca agatactatc caccaattct
     5161 ttgataaaca tcaaggtaaa gaattcctga ctatagtttc gaaagaatca tttgaaagaa
     5221 ataaaattta tgatcgagtc agaatccact attgggcacc acatattagt gaacgaactt
     5281 ttagtaataa gttgattggt aaattttttc aaaaatttat ccgatcttca gaattgtata
     5341 tgaaaagaat atgtagtgca gataaattta aaactttcgg tacggcttta ggatatgcct
     5401 cacagtgggt tagcattaca gatgagctag ttactatcct gctcgatgaa gaattatgga
     5461 ttagagatgt tttttctcat tcactatttt gtgatgaatt atttattcct acaattatta
     5521 ataaatatgg gtgggaatct aggatttatt attcaaaagg gatggtaaat gatccggagg
     5581 aatttcaagg taatttaaga tatattaatt ggtgggatgg ttctccttac acttggacag
     5641 attcgagtga agatttttcc tctttagaga aggctattaa tagagggcat ttctttgcta
     5701 gaaagtttga tatacaaact tacccagcaa tgatagattt tattaaggcg agaacgcagt
     5761 tttcttaaat ttatgagtta atacttctta ctaattcttt tcgtaattaa gactatggaa
     5821 tgagtgtttt ttgttttatg cctggataaa ttacagtatg aaagagggct taaaagaagt
     5881 aagttctggt gagttcagaa atcatttcct aagtagacta attttaggga agaaagttat
     5941 agctatagat gataataaat ttagatatta gttaatataa attagggcat tcttatgaga
     6001 aaatatgact atcttattgt tggagtaggt ctttttggga ctatttttgc ttatgaagcc
     6061 acaaaacgtg gtaaaaaatg tttggtaatt gaaaaacgtg accatattgg aggtaactgc
     6121 tatactcaaa atatagaagg tatcaacgta cataaatatg gtgctcatat tttccataca
     6181 agcaataaat tggtttggga ttacattcaa caatttgctg aatttaatcg ctttaccaat
     6241 tcaccggttg cacgttataa agatgaactc tatagcttgc catttaatat gttaaccttc
     6301 aataaaatgt ggggagtaat taaaccggaa gaagctgaag ccaaaattaa agagcaaatt
     6361 gctcaagagc atattacaga acctaaaaat ctggaagaac aagcaatctc attagttggt
     6421 cgtgatattt atgaaaaatt aatcaaaggc tatacggaaa aacaatgggg tagaaaatgt
     6481 acagagttgc ctgcttttat tattaaacgt ctacctgtgc gttacactta cgataacaat
     6541 tacttctatg atacttatca aggtattccg attggtggtt atacgggcat ttttgaaaga
     6601 atgctagatg gtatagaagt aaaacttggt gtagatttct ttcttgagcg tgaatattat
     6661 gagaatctag ctgataaaat tgtgtttacc ggaatgatcg atgaatactt tggttatcgg
     6721 tttggcagat tagagtaccg aagtttacgt tttgataacg aattattaga tataccaaac
     6781 tttcaaggta atgcggtagt taactatacg gaagcagaag ttccttatac ccgaattatt
     6841 gaatataaac attttgaata tggtatccaa cctaaaacag taatgacccg tgaatattcg
     6901 aaagaatatg aacaaggaga tgaaccatat tacccaatta atgatgcaag aaataatgag
     6961 ctttatgcta aatataaagt attagctgat gaaaaaagca atgtgatttt tggcggacgt
     7021 ttagctcagt ataaatattt tgatatgcac aatattattg ctgaggcctt agcttgtgta
     7081 gacaaacatt ttaagtagat aatttgcaag gggaatattt tgaataagat aatttctgcg
     7141 ataacattaa ttatttttga tatatttgcc atacttgttt caataatatg tgcagtatca
     7201 ttaagaaagg tattaaatgt tttttgggat atgccggtaa taacatattc ttatattaca
     7261 tttagtgccg tatatgttac tcttattctt cttcttgctt attttggtgt ctacaataga
     7321 agatttgagt tttggcacga aagcaagtta gtggttagag cttgcttttt atcttttatt
     7381 attttgtttg cagcattagc tttggggcag aatgcggatt tttattcaag aagtacatta
     7441 atactaatat tctgtatatc ttcaattaca attcctgtct ttaagatttt tgttaaaaag
     7501 atgttgttta atttaaaatt ttggcagaag ccggctaaga ttattacaga tagtgattct
     7561 tttagatttc agctttttaa tgatccttac ttaggttatg ttaaagcaga taatataaag
     7621 catgagacac tatttattga tagtacaaat attagtaaag ataaattaaa tagcattatt
     7681 gaggataata tcaaaaatag tagggaaatt attttttctc catttttagt aggttacgat
     7741 ttctctcaat caagtatata taatcttttt gactcaagaa taaatgtatt ccatttggaa
     7801 aataagttat taagcaaatt aaaccgattt tataaagcta taattgatta tacaattgtg
     7861 gttttgtcat cagttttttg gcttccttta ctttgtttaa tcgctatttg gattaagatt
     7921 gaagatccta aaggtgatgt attctttatg caaaggagat taggattaaa tggtaaagag
     7981 tttttatgct ataaattccg ttctatgtac tctgatcaat cttttatggc agattggtta
     8041 gaaatgaatc cggaagaaaa ggcttattat aatatttatc ataaatatat gaatgatcca
     8101 cgtattacta aggtaggttc tt
//
LOCUS       DANKBV010000104         8075 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.104, whole genome shotgun sequence.
ACCESSION   DANKBV010000104 DANKBV010000000
VERSION     DANKBV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.104"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1536)
                     /gene="mukB"
                     /locus_tag="PWY43_001857"
     CDS             complement(<1..1536)
                     /gene="mukB"
                     /locus_tag="PWY43_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDL2136717.1"
                     /translation="MIDTTELQNNPQIAPLARPNDVQRGKFLSLTLINWNGFFARTFD
                     LDDLVTTLSGGNGAGKSTTMAGFVTVLIPDLTLLNFRNTTEAGSTAGSRDKGLYGKLK
                     AGVCYAVLESFNSRGQRIITGVRLQQIVGRDKKVDIRSFSLQNIPQEQSIISLLTEQV
                     GNKARVLALNELKDQFENSEVQFKQYHSITDYHSFMFDLGVIPKRLRSAADRSKFYKL
                     IEASLYGGISSVITKSLRDYLLPENTGVRQAFQDMESALRENRMTLEAIKVTQSDRDM
                     FKRLITESTNYVSADYMRNANERRGNVQQALEQRKEWYAAKSKILLEQQRFVEFSRES
                     ADIAEAEQALEADYNSANDHLNLVMNALRHQEKIERYQDEVEELNIKLEEQQEALEEI
                     AEIAENAQARADEADDYVEELRSQMADYQQALDAQQTRALQYQQAVNALEKAKQLTGL
                     VNLDLNNIEDYHAEFVAQAEDLTDQVFELEQRLSVSDMAKTQFEKAFESVCKISGEID
                     RLQA"
     gene            complement(1712..2428)
                     /gene="mukE"
                     /locus_tag="PWY43_001858"
     CDS             complement(1712..2428)
                     /gene="mukE"
                     /locus_tag="PWY43_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDL2136718.1"
                     /translation="MTENIQDFISPKLALAIANPIFPELDSQLRAGRHINTEQLDQHV
                     FLMDFQAELESFYRRYHVELIRAPEGFFYLRPKASTLIARSAMSEMEMLVGKVLCYLY
                     LSPERLAQQGIFSQDDVYEELLSLADESKLLKAVNPRSTGSDLDRAKLAEKVGGALRR
                     LARIGIITRVGEQNSKKFVISESVFRFGADVRTGDDPREAQLRLIRDGEATTPALLNE
                     QAVEIDENIADEAPFDGENE"
     gene            complement(2477..3820)
                     /gene="mukF"
                     /locus_tag="PWY43_001859"
     CDS             complement(2477..3820)
                     /gene="mukF"
                     /locus_tag="PWY43_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDL2136719.1"
                     /translation="MQNELAQTIPELISWTKDREFSLSLSSDRLAFLLAISIYNQEQT
                     DGELLESDLIDLFRYVSNSFEQSDATFTQRANNAINDLVRQRLLNRFSSEFTEGLAIY
                     RVTPLGVGISNYYVRQREFSTLRLSIQLSIVADEIQRASVAAEEGIEQAQDERFWRNN
                     VFAPLKYSVAGIFDSIDLSQRIMDENQHQIRERIAELLSQNWHEAIMSCEQLLDETSG
                     NLRELQDTLNAAGDKLQAQLLRIQSCLMARNDLDFIDQLIVNLQNKLDRIISWGQQAI
                     DLWIGYDRHVHKFIRTAIDMDKNRVFGQRLRQSIQDYYNSPWLLYTAKADSLLDLRDD
                     EAMLNEAESVGELPSELEYESLSDVQEQIVSAMQAHLAPFRAEGKPIDLGVILREQLA
                     RFPESRHFDVARIIVDQAVKLGMASQDSQAVYPQWQPINEQGAEVQANVIDQYNK"
     gene            complement(4078..4965)
                     /gene="galU"
                     /locus_tag="PWY43_001860"
     CDS             complement(4078..4965)
                     /gene="galU"
                     /locus_tag="PWY43_001860"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2136720.1"
                     /translation="MKVIIPVAGLGTRMLPATKAIPKEMLTLADKPLIQYIVNECVAA
                     GFKEIVLVTHSSKNAIENHFDTSFELETMLEKRVKRQLLDDVRSIVPKDVTLIHVRQG
                     QAKGLGHAVLCGRTVVGDEPFAVVLPDVLLGEFTANQKTENLAAMVKRFQETGYSQIM
                     VAPVPMENVSSYGVADCHGVDIPLGGAAPIAKMVEKPSIEEAPSNLAVVGRYVFSSNI
                     WNLLEKTPVGVGDEIQLTDAIDMLIHQETVEAFHMTGRSFDCGDKVGYMKAFVEYSLN
                     HDKCGKEFKAFIKELAKSL"
     gene            complement(5101..5286)
                     /gene="csrA"
                     /locus_tag="PWY43_001861"
     CDS             complement(5101..5286)
                     /gene="csrA"
                     /locus_tag="PWY43_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDL2136721.1"
                     /translation="MLILTRKIGEVLLVGDDVEITVLSIRGNQVKLGVNAPKEISVHR
                     REIYERIKASVEEENAE"
     gene            complement(5376..8003)
                     /gene="alaS"
                     /locus_tag="PWY43_001862"
     CDS             complement(5376..8003)
                     /gene="alaS"
                     /locus_tag="PWY43_001862"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL2136722.1"
                     /translation="MKTTSDIRQSFLEFFQTKGHTIVPSSSLVPENDPTLLFTNAGMN
                     QFKDVFLGLEKRPYSRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYF
                     KQDAIKFGWEFLTSPQWLGLPKEKLYVTVYETDDEAYDIWHKEVGVPSEHIIRIGDNK
                     GAPYASDNFWAMGDTGPCGPCTEIFYDHGPEHWGGLPGSPEEDGDRYIEVWNIVFMQF
                     NRLADGTMEKLPKPSVDTGMGLERMTAVMQHVNSNYETDIFQTLIKEVATLLNVKDLD
                     NKSLRVVADHIRACSYLIADGVLPSNEGRGYVLRRIIRRAVRHGNILGTKEAFFYKLV
                     PTLATVMGQAGEVLTQKQAHIQKTLKAEEEQFARTLERGLNLLEEALAKVENNTLSGE
                     VAFKLYDTYGFPLDLTADVCRERDIKIDEDGFNAEMTAQRERAKASSSFGTDYNNVIK
                     VDSKTCFSGYTETSLNDATVIGLFSNGKSVESIQSGENAVVILDKTPFYAEMGGQVGD
                     SGQISAEICNFEVVDTQKYGQVFGHIGQLTSGSLSVGDKVNTIVDRERRHAITLNHSA
                     THLLHSALRQVLGDHVAQKGSLVSENILRFDISQPEAITKSQLEEVERIVNAKVRENI
                     QVIIEEMDIESAKAKGAMALFGEKYGEVVRVVEMSDFSIELCGGTHVKQTGDIGLFKI
                     TSEGAVAAGVRRIEAVTGENAIAWLHTLQQAVQQSAELLKADSHSFVEKIVQLQEKAK
                     RTEKELQQLKDKLAAQAGSDLAKQATQINGVNVVIQQLENVEPKALRTMVDDLKNQLG
                     SAVVVFATTAEDKVNLIVGVTKDLTDKVNAGQLVGAMAEKVGGKGGGRPDMAMAGGSE
                     PQHLLQALSLAQEWITAKL"
BASE COUNT         2236 a         1858 c         1503 g         2478 t
ORIGIN      
        1 cgcttgcaag cggtcaattt caccggaaat tttgcaaacc gattcaaacg ctttttcaaa
       61 ttgggttttc gccatatccg acacgcttaa acgttgttcc agctcaaata cttgatcggt
      121 aagatcttcg gcttgagcca caaattctgc gtggtaatct tcaatattat ttaaatctaa
      181 attcactaag ccggttagct gtttggcttt ttctaacgca ttcaccgctt gttggtattg
      241 caatgcacga gtttgctgtg catctaaggc ttgttggtaa tccgccattt gagaacgcaa
      301 ttcttcaacg taatcatctg cctcatccgc acgagcctga gcattttcgg caatttccgc
      361 aatttcttcc aatgcctctt gctgttcttc cagcttaata ttcagctctt caacttcgtc
      421 ttggtagcgt tcgattttct cttggtggcg taacgcattc atcactaaat ttagatgatc
      481 gttcgcacta ttgtaatccg cctctaacgc ctgctctgct tcggcaatat ccgcactttc
      541 acggctaaat tccacaaagc gttgctgttc gagcaaaatt ttggattttg ccgcatacca
      601 ctctttgcgt tgctctaagg cttgttgtac attgccacgg cgttcattcg cattacgcat
      661 ataatccgcc gacacataat tggtcgattc ggtaattaag cgtttgaaca tatcccgatc
      721 cgattgcgtg accttaatcg cctctaaggt catacggttt tcacgcagag cggattccat
      781 atcttggaac gcttggcgaa cgcccgtatt ttccggcaac aagtaatcac gcaaggattt
      841 ggtgatcaca cttgaaatac caccataaag cgaggcttcg attaatttgt agaatttgct
      901 acggtcggct gccgaacgaa ggcgttttgg gattacgccc aaatcgaaca taaaactgtg
      961 gtaatcagta atcgaatggt actgtttgaa ttggacttcg ctgttctcaa attgatcttt
     1021 gagttcattt aacgccagca ctcgagcctt attccccact tgttcggtca gtaggctgat
     1081 aatgctttgc tcttgaggaa tattctggag cgagaacgaa cgaatatcga cttttttatc
     1141 ccgtccgaca atttgttgca aacgcacgcc ggtaataatc cgttgcccac gggaattgaa
     1201 tgactctaac accgcatagc aaacacccgc ttttaactta ccgtataagc ctttatcacg
     1261 agaaccggcg gttgagcctg cttcagtcgt attacggaaa ttaagcaagg ttaaatctgg
     1321 gatcaagacg gtcacaaacc ctgccattgt ggtagactta cctgccccgt taccgcctga
     1381 aagcgtggtc actaagtcat ctaaatcaaa ggttcgagca aaaaaaccgt tccagttaat
     1441 cagcgttaaa gagagaaatt taccgcgttg tacatcattc gggcgtgcaa gcggtgcaat
     1501 ttgaggatta ttttgcaatt ctgtggtatc aatcattatg ttttctcact aattgtccta
     1561 gcacgctaaa gcgtactagg ttaggtatag gtgagtagct ccgctactct aatcgcaacc
     1621 tcagccttgg agggctgctt tatgcttaac ctagttcggc tttaccgtgt ctagggcaga
     1681 ctcattaaat gctgaatcta ttcgaatgaa atcactcatt ttctccgtca aagggggctt
     1741 catctgcaat attttcatct atttcaaccg cttgttcgtt taacaacgca ggggttgtcg
     1801 cttcaccatc acgaattaag cgaagctgtg cctctcttgg gtcatcaccg gtacgcacat
     1861 cagccccaaa acggaagacc gattctgaaa tcacaaattt tttgctgttt tgctcgccta
     1921 ctcgggtaat aatgccgata cgggctaatc tgcgtaacgc tccgccgact ttttctgcca
     1981 gcttggcacg gtctaaatcg gaaccggtcg aacgtgggtt taccgctttt aataatttgc
     2041 tttcatcggc aagactgagt aattcttcat acacatcatc ttggctgaaa atcccctgct
     2101 gagccaaacg ttccggactt aaatagaggt agcacaacac cttacccact aacatttcca
     2161 tttctgacat tgccgaacgg gcaattagtg tcgaggcttt cgggcgtagg tagaaaaagc
     2221 cttccggtgc tcgaatcaac tcaacgtgat aacggcggta aaaactctct agttccgctt
     2281 gaaaatccat caaaaagacg tgttggtcaa gctgctcggt attgatatgg cgaccggctc
     2341 gtaattggct gtctagctcc ggaaaaatcg ggttggcaat cgcaagtgcc agtttcgggg
     2401 aaatgaaatc ttggatattc tctgtcattg tcattctctt caattttgca aaattctggg
     2461 taaaaacgac cgcttgttat ttattgtatt ggtcgataac attcgcctgc acttccgccc
     2521 cttgctcgtt aatcggctgc cattgtgggt aaacggcttg gctatcttgg cttgccatac
     2581 ctaacttaac agcttgatcg acaataatac gagccacgtc aaaatggcgg gattccggga
     2641 atcgggcaag ctgttcacgc aagataacgc ctaaatctat cggtttacct tcagcacgga
     2701 acggcgctaa atgagcttgc attgccgaaa caatttgctc ttgcacatct gaaagcgact
     2761 catactctaa ctcacttggc aactcgccca ccgattctgc ctcatttaac attgcttcat
     2821 catcacgcaa gtccagtaac gaatcggctt ttgcagtata aagcaaccaa gggctgttgt
     2881 aataatcttg aatcgattgg cgtaaccgtt gcccgaatac acggtttttg tccatatcaa
     2941 tcgctgtacg aataaattta tgcacatggc ggtcatagcc gatccaaaga tcgatagctt
     3001 gttgtcccca gctgataata cggtctaact tattttgtag attcacaatc agctgatcga
     3061 taaaatccaa atcattacga gccatcaggc aactttgaat ccgcaataat tgagcttgca
     3121 gtttatcacc agcggcattt aaggtatctt gcaactcacg caaattcccc gaggtttcgt
     3181 ctaacaattg ctcacaactc ataattgctt cgtgccagtt ttggctaagc agttcggcta
     3241 ttcgttcacg gatttggtgc tgattttcgt ccataatccg ttgtgaaaga tcgatgctgt
     3301 caaagattcc ggccaccgaa tatttcaatg gggcaaacac attatttcgc caaaaacgtt
     3361 catcttgtgc ctgctctatt ccctcttccg ctgctactga agctcgctga atttcatctg
     3421 ccacaatcga aagttgaatc gataaacgca gggtagaaaa ttcccgctgg cgaacgtaat
     3481 aatttgaaat tcccacccct aacggcgtaa cacgataaat cgccaaacct tccgtaaatt
     3541 cgctactaaa acggtttaac aaccgttgtc tgactaaatc attaatcgca ttattggcac
     3601 gctgggtaaa agtggcatca gattgctcaa acgaatttga aacataacgg aaaagatcaa
     3661 taagatcact ttctaacaat tcaccatctg tctgttcttg gttataaata gagatagcaa
     3721 gtagaaacgc taagcggtcg gaagaaagcg aaagtgaaaa ttcacgatct ttcgtccaag
     3781 agatgagttc tggaatagtc tgtgccaatt cgttctgcat acgggcttaa tctttaattt
     3841 ctatacaaaa atggtattta ttgtaactga atgtaatcaa aatgggaatg gataaaacgc
     3901 agacaagaga aatgaacaat ataagcggtc aaatatgggt gattttttgc aaaaagtatt
     3961 ttaaaggtaa atgaatccga aaatttatta tcactacgct aatttatgat accataaata
     4021 gaaaggactg gatccaatag aatatcagac cttagatcat agtctaaact ctaaggctta
     4081 gagtgattta gctaactctt taataaatgc tttaaattct tttccgcatt tgtcatgatt
     4141 taaactatat tctacaaatg ctttcatata gccgacttta tcaccacaat caaatgagcg
     4201 ccctgtcata tgaaaagctt caaccgtttc ttggtgaatc aacatatcaa ttgcatccgt
     4261 cagctggatt tcatcaccta cgcctactgg cgttttttct aataaattcc aaatatttga
     4321 ggaaaataca taacgtccta ctaccgctaa attagaaggc gcctcttcaa tacttggttt
     4381 ttcaaccatt ttcgcaatag gcgctgcccc cccgagaggg atatctacac catggcagtc
     4441 agcaacccca tagctactta cattttccat tggaaccggc gcaaccataa tttggctata
     4501 tccggtttct tggaaacgct tcaccatcgc agctaaattt tccgtttttt ggtttgctgt
     4561 aaattcacca agtaatacat ctggtaatac aaccgcaaac ggttcatcgc caaccactgt
     4621 tcgtccacat agaaccgcat gacctaaacc tttggcttgc ccttggcgaa cgtgaattaa
     4681 ggtaacatct tttggtacaa tagaacgtac atcatctaat aattgacgtt ttactcgttt
     4741 ctccagcatc gtttccaatt caaaagaggt atcaaaatgg ttttcgatag cattctttga
     4801 agagtgagta accaatacaa tttctttaaa acctgcagcc acacattcat ttacaatgta
     4861 ttgaataaga ggtttatctg caagggtaag catttctttt ggaatagctt tggttgcggg
     4921 taacatgcgc gttcccaatc cagcaacagg aataatgact ttcatttgga atcctttgat
     4981 gacttaacat attagatata actgaaataa accgcttaga cataacaagc ggtcaatttt
     5041 cagaaaattt tgcattgcct gcttcgctgt tatcatctag cagctttaca gttaaaatgc
     5101 ttattccgca ttttcctctt ctactgaggc ttttattctt tcataaatct ctcggcggtg
     5161 tacagagatt tcttttggtg cattcacacc taatttaact tgatttcctc ggatgctgag
     5221 taccgtaatc tcaacatcat ctcctacgag taaaacttcc ccaatcttac gagttagaat
     5281 aagcataatt gtctcctaaa aagtgaaaaa ctctataaat acttaaatat agagtaaagt
     5341 tttaaaggcg agttcagctc gccttgttat tatttttaaa gttttgctgt aatccattct
     5401 tgagcaagtg acaatgcctg caataaatgt tgtggttctg agcctccggc cattgccata
     5461 tcaggacgtc cgcccccttt acctcccact ttttcagcca ttgcaccaac taattgaccc
     5521 gcattgactt tatcggttaa atctttggtc acacctacaa tcaaattgac tttatcttcc
     5581 gccgtagtcg caaatacaac aactgcactg cctaattgat tttttaggtc atcaaccatc
     5641 gttctcaatg cttttggttc aacattctct aattgttgaa tcactacatt cacgccattg
     5701 atttgggtag cttgtttggc taaatctgaa cctgcttgtg ctgcaagttt atcttttagc
     5761 tgttgaagct ctttttctgt acgtttagcc ttctcttgta attgaacaat tttctcaacg
     5821 aatgaatgac tgtctgcttt taacaattca gcactttgtt gaacggcttg ttgtaaggta
     5881 tgcaaccaag caatcgcatt ttcaccagtg acagcctcta tacggcgtac acctgctgct
     5941 accgcacctt cagaggtaat tttgaataag ccaatatcac cggtttgttt aacgtgtgtg
     6001 ccgccacaaa gttcaatcga gaaatctgac atttccacta cacgcactac ttcgccatat
     6061 ttttcaccaa ataacgccat tgcacctttc gcttttgcag attcaatatc catttcttca
     6121 atgattacct gaatattttc acgcacttta gcattcacga tacgttcaac ttcttcaagt
     6181 tgtgatttag taattgcttc cggctgagaa atatcaaaac gtaaaatatt ttctgacact
     6241 aatgagcctt tttgagcaac gtggtcgcct agcacttggc gtaacgcaga atgcagtaag
     6301 tgggtagcac tgtggtttaa tgtaatagca tgacgacgct ctctatctac gatcgtatta
     6361 actttgtcgc caacagaaag tgagccggaa gttaattgcc caatatgccc gaatacttga
     6421 ccatattttt gcgtatctac tacctcaaaa ttgcaaattt ctgcggaaat ttgaccgcta
     6481 tctcccactt gtccgcccat ttctgcatag aatggggttt tatctaaaat cactaccgca
     6541 ttttcacccg attgaataga ctcaaccgat tttccattac taaataaccc aattacagta
     6601 gcatcattta agctggtttc agtatatcca ctaaagcatg ttttactgtc cactttaatc
     6661 acattgttat aatctgtgcc aaagctactg ctggctttcg cacgctcacg ttgtgcggtc
     6721 atttctgcat tgaagccgtc ttcgtcaatt ttaatatcac gttcacggca aacatctgcg
     6781 gttaaatcta acgggaagcc ataagtatca taaagtttga acgcaacttc gccagataag
     6841 gtgttatttt caaccttcgc taatgcttct tctaataaat tcaatccacg ctcaagagta
     6901 cgggcaaatt gctcttcttc tgctttgagt gtcttttgaa tatgcgcttg tttttgggtc
     6961 agcacctcac cagcttgccc cattaccgtt gcaagtgttg gcaccaattt atagaaaaag
     7021 gcttcttttg tacccaaaat attaccgtga cgaaccgcac ggcggataat acggcgtaat
     7081 acatagcctc ttccttcatt tgaaggtaaa acaccatcgg caatcagata ggaacaagca
     7141 cgaatatggt ctgccactac acgtaaagat ttattatcta aatctttcac attaagcaaa
     7201 gtagcgactt ctttaattaa agtttggaaa atatccgttt cgtagttgga gtttacgtgt
     7261 tgcattaccg cagtcatacg ttctaacccc atccctgtat ctacagaagg cttaggcagt
     7321 ttttccatcg taccgtctgc taaacgattg aactgcataa atacaatatt ccatacctca
     7381 atatagcggt cgccatcttc ttctgggcta cccggtaagc caccccaatg ttcaggcccg
     7441 tgatcataga aaatctcggt acaagggcca caagggccgg tatcgcccat cgcccagaag
     7501 ttatctgaag cataaggtgc gcctttatta tcgccaatgc gaataatatg ttccgatggt
     7561 acacctactt ctttatgcca aatatcgtag gcttcatcat cagtttcata cacagttaca
     7621 tataattttt cttttggtaa gccaagccat tgtggagaag ttaaaaactc ccaaccgaat
     7681 ttgattgcat cttgcttgaa gtaatcacca aagctgaaat tccccatcat ttcaaaaaaa
     7741 gtatgatggc gagcggtata acctacgttt tctaaatcat tgtgtttacc acctgcacgt
     7801 acacaacgtt gtgccgtagt cgctctgcta taagggcgtt tttctaaccc aagaaaaaca
     7861 tctttgaatt ggttcatgcc cgcattggta aataataatg tcggatcatt ctcgggaaca
     7921 agagagctgc ttggaacaat cgtatgtcct ttagtttgga aaaactctag aaaagattgt
     7981 ctgatatctg aagttgtttt cattaaaaat cactcttttt gtaaatagaa gttaaacatt
     8041 tagttatctt gctattttac acatttttta tgcta
//
LOCUS       DANKBV010000105         8034 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.105, whole genome shotgun sequence.
ACCESSION   DANKBV010000105 DANKBV010000000
VERSION     DANKBV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.105"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..754
                     /gene="proA"
                     /locus_tag="PWY43_001863"
     CDS             <1..754
                     /gene="proA"
                     /locus_tag="PWY43_001863"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL2136723.1"
                     /translation="ALEKAGLPKLAVQAVTDPDRALLLELLKLDRYIDMVIPRGGAGL
                     HQFCKENSTIPVIVGGIGVCHLFVEKTADQEKSLEVIANAKTQRPSTCNTLETLLVEK
                     AIAVEFLPKLVAKMQQLGVTIHSNDLQKIEGIEPLDQAKLSQEWLSLDLNVVVVNDLQ
                     QAVEHIRHYGSQHSEGILTSDYQLARQFVQQVDATAVYINASTRFTDGGEFGLGAEVA
                     VSTQKLHARGPMGLEALTTYKWVCEGDYLVRG"
     gene            756..1601
                     /locus_tag="PWY43_001864"
     CDS             756..1601
                     /locus_tag="PWY43_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDL2136724.1"
                     /translation="MLNRLWRFIKLFAKRYNENNIAISAGYLTYSTMLAIVPFIVVLF
                     SIFTFFPIFHEANEQLKAFIYDNFAPSASEMVSEYIELFVSNAKQMGLVSFIGLFVVT
                     IMLIKSIDEALNTIWHDNRQRPMWFSFLLYFLILLFGPLLAGASIAISTYVMSLSIFN
                     GSGALSLGQHLLELTPFLLIWLLFTLVYKFIPIRKVKFIHAAIGALMAAIFFTLGKKA
                     FIWYITAFPSYQAIYGALAVLPIMLLWIQLSWQVVLLGGQFAAVLDEMETDKGSVNDR
                     LNSAG"
     gene            1576..2010
                     /gene="dtd"
                     /locus_tag="PWY43_001865"
     CDS             1576..2010
                     /gene="dtd"
                     /locus_tag="PWY43_001865"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL2136725.1"
                     /translation="MIGLIQRVKFAKVEVEGQVVGEIASGLLVLLGVEKDDDEAKADK
                     LLEKVLNYRVFSDSEGKMNLNVQQAGGSLLVVSQFTLAADTNKGLRPSFSKGASPVDA
                     ERLYAYFHQQAATKIHTQTGQFAADMQVSLQNDGPVTFWLQV"
     gene            complement(2093..2917)
                     /gene="mutM"
                     /locus_tag="PWY43_001866"
     CDS             complement(2093..2917)
                     /gene="mutM"
                     /locus_tag="PWY43_001866"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDL2136726.1"
                     /translation="MPELPEVETSVRGVSPYLVGQTIEQIIVRQPKLRWAVSPELSQM
                     AGAKITAVERRAKYLIIRTDKGDILVHLGMSGSLRILSQTADIAFGKHDHIDLVTTNG
                     TILRYNDPRKFGCWLWAEKAEDHELLRKLGPEPLSNDFTADYLFAKSRKKTTACKTFI
                     MTNEVVVGVGNIYACESLFMAGIHPELAAQNLTKKQCERLVKTIKEVLTKAIIQGGTT
                     LKDFIQPDGKPGYFSQVLQVYGRKGEACNDCGKTIETKVIGQRNTYFCPKCQRLPK"
     gene            complement(2963..4261)
                     /gene="brnQ"
                     /locus_tag="PWY43_001867"
     CDS             complement(2963..4261)
                     /gene="brnQ"
                     /locus_tag="PWY43_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2136727.1"
                     /translation="MTRNTLVVGFLLFAIFFGAGNLIFPPKLGYESSTEFFSAISGFV
                     LTGVGLPLLGIIVSAFYNGGYKSALDKIHPWFSLAFLTSIYLAIGPFFAGPRTAATAY
                     EMAIMPFLGDSSQVSMLIFTLIYFAFTLWLALNPNKMVDRIGAILTPILLITIIGLVI
                     RAGFILDTDPQITTPNTNEQSYFFKGMVDGYLTMDALASLAYSVIVLNAIRGKGVSQA
                     ALQRQTVSAGLVAAVALAVIYISLGWIGNNMPISADTLSMLSERKLDLGTYILNTVTT
                     QAFGELGRSVLGVIVSLACLTTAVGLAVSVSEYFNEIFPKISYKVYAILFTVISFTVA
                     NQGLKEVISKSIPVLLVLYPIAMTVILMLLINLVIKLPLSAQRLSLLLVTIVAIASVA
                     GVGFTENLPLKSYSMEWLPFAIIGAIIGAIIHPLTGRKHK"
     gene            complement(4374..5096)
                     /gene="fabG"
                     /locus_tag="PWY43_001868"
     CDS             complement(4374..5096)
                     /gene="fabG"
                     /locus_tag="PWY43_001868"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL2136728.1"
                     /translation="MSKIALVTGATRGIGKAIAEELVAKGYIVVGTATSEKGAESISA
                     YLGENGKGLVLNVADAESIESVLKQIKETFGDVDVLVNNAGITRDGLLMRMKDEDWFD
                     IIQTNLTSVYRLSKAVLRPMMKKGGRIITIGSVVGSMGNSGQTNYCAAKAGLVGFSKS
                     LAKEVASRGITVNVVAPGFIATDMTDELTEDQKNAILSQIPAGELGSPQDIAKAVAFL
                     ASDDARYINGETIHVNGGLYMS"
     gene            complement(5117..5581)
                     /locus_tag="PWY43_001869"
     CDS             complement(5117..5581)
                     /locus_tag="PWY43_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136729.1"
                     /translation="MSKETLFMLISALIALVILPLAYTVGIAKETLGGNMGMLSIAAG
                     CSFLAYFLSLNPKIRHAFIGGSAITAAAIQAWVLYGVLLSENDPKSAVVLFQIYLFSS
                     LGPLFFSAIWAFTRPRWIYPKQGSAVKYALFGGLTTVINYLITLFIFYILTL"
     gene            complement(5598..6533)
                     /gene="fabD"
                     /locus_tag="PWY43_001870"
     CDS             complement(5598..6533)
                     /gene="fabD"
                     /locus_tag="PWY43_001870"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2136730.1"
                     /translation="MSKFAMVFPGQGSQAVGMLADLAPQYPIVEETFKQASDVLGYDL
                     WDLVQNGTAEELGQTHRTQPALLASSVALFRIWQQKFPEQKPAVMAGHSLGEYSALVC
                     AGVLDFQDAIKLVELRGRAMQEAVPAGSGAMFAIIGLDNESIIKACEQAASETGEIVS
                     AVNFNSPGQVVIAGTKAAAEKAGELCKAAGAKRALPLAVSVPSHCALMKPAAEKLAEA
                     LQNIELNQPLVPVINNVDVAVETDADTIRNALVRQLYSPVRWTETVEKMAQDGITTLY
                     EIGPNKVLTGLAGRIVKELSAKAVNDVASFEAVEA"
     gene            complement(6950..7705)
                     /locus_tag="PWY43_001871"
     CDS             complement(6950..7705)
                     /locus_tag="PWY43_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL2136731.1"
                     /translation="MTEQNQQNQQPQEHIESVRARRRAQNQKKDPNAPFVREKLTLPN
                     GHNKLLLHSCCAPCSGEVMEAIHASGIEFTIYFYNPNIHPLKEYLIRKEENIRFAEKW
                     GIPFIDLDDDYENDRKEWFKKAKGMEWEPERGIRCTMCFDMRFEKAAEYAHNNGFPVF
                     TSCLGISRWKDMNQINDCGHRAAAKFDDVVYWDYNWRKGGGSQRMIEISKRERFYQQE
                     YCGCVYSLRDSNKWRLETGRQRIEIGKLYYSPD"
     gene            complement(7808..>8034)
                     /locus_tag="PWY43_001872"
     CDS             complement(7808..>8034)
                     /locus_tag="PWY43_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDL2136732.1"
                     /translation="TQAELIRNAIENGGELEQIPQVLAIMQEHQSLDYAMQKAREEAQ
                     KAVDSLGVLPESEYKQALVSLAYLSVDRSY"
BASE COUNT         2213 a         1708 c         1745 g         2368 t
ORIGIN      
        1 tgcgttagaa aaagccggct tgccgaaatt ggcagtgcaa gcggtgacgg atccggatcg
       61 agccttatta ttggaactat tgaagctaga tcgctacatt gatatggtga tccctcgagg
      121 cggtgcaggt ttacatcaat tctgtaaaga aaattcgacc attccggtga ttgtgggggg
      181 gattggggtt tgtcatctgt ttgtggaaaa aacagcggat caggagaaat ctctagaggt
      241 catcgctaat gccaaaacgc aacgcccaag cacttgcaat acgctggaaa cgctgttggt
      301 ggaaaaagcg attgcggtgg aatttttgcc gaaattggtc gccaaaatgc aacagcttgg
      361 cgttactatt cacagtaacg atttgcaaaa aattgaagga attgaaccgc ttgatcaagc
      421 aaaattgagc caagagtggt tatcgcttga tcttaacgtg gtggtggtga atgacctgca
      481 acaagcggta gagcatatcc gccattatgg ttcgcagcat tcagagggga ttttaacctc
      541 cgattatcaa cttgcccgtc aatttgtaca acaggttgat gccacggcgg tttatattaa
      601 tgccagcacc cgttttaccg atggtggtga gtttggttta ggggcagaag tggcagttag
      661 cacgcagaaa ttacacgcac gaggcccgat gggattagaa gccttaacca cctacaaatg
      721 ggtgtgtgaa ggcgattatt tggtgagagg ttaagatgtt aaatcgactg tggagattta
      781 ttaagctctt tgccaagcgt tacaatgaaa ataacattgc gatttcagcc ggttatctca
      841 cttatagcac aatgctggcg atagtgccgt ttattgtcgt gctgttttcc atttttacct
      901 tttttccgat tttccacgaa gcaaacgagc agttaaaagc gttcatttat gacaactttg
      961 ccccgagtgc gagcgagatg gtgtcggaat acattgagtt gtttgtcagt aatgctaaac
     1021 aaatggggct ggtgagtttt atcggcttat ttgtggttac tattatgctg attaagagta
     1081 ttgatgaagc attgaatacc atttggcacg ataatcgtca gcgcccgatg tggttctctt
     1141 ttttgcttta ttttctcatt ttgctgtttg ggccgctgct tgccggcgcg agtattgcaa
     1201 ttagcaccta tgtgatgtcg ctttctattt ttaatggaag tggggcgtta tcgctcgggc
     1261 agcatttatt agaattgacg ccgtttttat taatttggtt actttttacc ttagtgtata
     1321 aatttattcc gattagaaag gtaaaattta tacacgctgc tatcggggca ttaatggcag
     1381 cgattttctt taccttaggg aagaaagcct ttatttggta tattactgct tttccgtctt
     1441 atcaggcaat ttatggagca ttagcggtgt taccgattat gctgttatgg attcagttaa
     1501 gttggcaggt ggtattactt ggcggacaat ttgcggcggt tttagacgaa atggaaacag
     1561 ataaggggag cgttaatgat aggcttaatt cagcgggtta aattcgcaaa agtggaggtt
     1621 gaagggcaag tcgttggcga aattgcaagc ggtttgttgg tgttgctcgg tgtggaaaaa
     1681 gacgatgatg aagcgaaagc agataagcta ctggaaaaag tgctgaatta tcgggtgttt
     1741 tcggatagcg aggggaaaat gaatttaaac gtacagcaag ccggtggcag cttattagtg
     1801 gtttcacagt ttacccttgc ggcggatacc aataaaggct tgcgtcctag tttctcaaaa
     1861 ggggcaagcc cggttgatgc cgaacgtttg tatgcttact ttcatcaaca agccgccact
     1921 aaaattcaca cccaaaccgg acaatttgca gccgatatgc aagtcagcct acaaaatgac
     1981 gggcctgtta ccttttggtt gcaggtttag gttttaccct ctctctgctt gtttcactta
     2041 acgtttcaac aagctgtctc tctccctcag ggggagagag tttattatta gattatttcg
     2101 gcaatctctg acacttcggg caaaaatagg tattacgctg cccgattact ttggtttcaa
     2161 ttgtctttcc gcagtcatta caggcttccc cttttcttcc atacacttgc aacacttgag
     2221 agaaatagcc cggtttgcca tcaggttgga taaaatcttt cagtgttgtg ccgccttgaa
     2281 taatcgcttt cgttagtact tctttgatgg tttttactaa acgttcgcac tgttttttag
     2341 tcaggttttg tgctgcaagc tcagggtgaa tgcctgccat aaataaactt tcgcacgcat
     2401 agatattccc caccccaaca accacttcat tagtcataat aaaggtttta caagcggtcg
     2461 tttttttgcg gctttttgca aataagtaat ctgctgtgaa atcgtttgaa agcggttcag
     2521 gcccgagttt acgcaacagt tcgtgatctt ctgctttttc tgcccatagc caacaaccga
     2581 atttgcgagg gtcgttgtag cgtaaaattg tgccattggt ggtgactaaa tcgatatggt
     2641 cgtgcttacc gaaagcgata tcggcggttt gagacaaaat cctgagcgag ccggacattc
     2701 ctagatgcac caaaatatcg cctttgtcgg tgcggataat cagatatttg gcacgtcttt
     2761 ctactgcggt aatttttgcc cctgccattt ggcttaattc aggactcacc gcccaacgta
     2821 actttggctg acgaacgatg atttgttcaa tagtttgccc aaccaaatag ggcgatacac
     2881 cacgaacgct ggtttcaact tcgggtaatt caggcattat tctctcctaa aaaagaggca
     2941 gtgaatgctc actgccttta ctttatttat gctttcgacc ggtaagcggg tggataattg
     3001 ctccaataat agctccaatg atagcgaaag gtagccattc catcgagtag gattttagag
     3061 gcaagttttc ggtaaacccg acaccggcaa ctgaagctat cgctacaatt gtcactaata
     3121 ggagtgatag acgttgtgca gacaacggaa gtttaatcac taaattaatc agcaacatta
     3181 aaatgacagt cattgctatt gggtagagaa ctaataatac cggaatggat ttgctaataa
     3241 cttcttttaa tccttggtta gcaacagtaa aactaatcac agtaaataag atagcataaa
     3301 ctttatatga aattttcggg aaaatttcat taaagtattc actgactgat acggctaaac
     3361 cgacagcagt ggttaaacag gcaagtgaaa caatcactcc taagacacta cgtccgagtt
     3421 caccgaaggc ttgggtagtc acagtgttaa gaatgtaagt acctagatcc aattttcttt
     3481 ctgataacat cgaaagtgtg tctgcactaa ttggcatatt attaccaatc caacccaatg
     3541 aaatataaat tacagctaga gcaactgctg caactaagcc ggcagaaact gtttggcgtt
     3601 gtaatgcggc ttggcttacc cctttcccac gaatagcatt taatacaatt accgaatagg
     3661 ctaacgaagc aagtgcatcc atagttagat agccatcaac cataccttta aaaaagtaag
     3721 attgttcatt cgtattaggc gtagtgattt gtggatcggt atctaaaata aagccggctc
     3781 gaataactaa tccaatgatg gttataagca agatcggggt aagaattgca ccaatgcgat
     3841 caaccatttt atttggattt agcgctaacc atagggtaaa ggcaaagtaa attaaggtaa
     3901 aaattaacat tgacacttgg ctggaatccc ctaaaaacgg cattatcgcc atttcatagg
     3961 cagttgctgc cgtgcgcgga ccggcaaaga atggaccgat cgctaaatag atagaagtta
     4021 ggaatgctaa agaaaaccaa gggtggattt tgtctaatgc agatttatag cctccattat
     4081 aaaatgcact gacgataata ccgagtagag gaagccctac tccagttaaa acaaagcctg
     4141 aaatggctga gaaaaattct gtactacttt cataacctaa ttttggtggg aaaatcagat
     4201 tgccggcacc aaagaaaata gcaaacaata aaaagcctac tactaatgta tttctagtca
     4261 taacatattc acactttttc acctataaag caggtgataa cacaaaataa caaataacaa
     4321 aaccgaactc tgaatttaca aagctcggtt gaaaaaagac cgcttgtagc ggattagctc
     4381 atatataaac cgccattaac gtgaatggtt tcaccgttga tataacgggc atcatcgctg
     4441 gctaagaaag cgactgcttt ggcaatatct tgtggagaac cgagttcacc ggcaggaatt
     4501 tggcttaaaa tcgcattttt ctgatcttcg gttagctcat cggtcatatc agtggcgata
     4561 aagcccggag caaccacgtt taccgtaatg ccgcggcttg ctacttcttt cgctaaagat
     4621 ttagaaaaac caactaagcc tgctttggca gcacagtagt tggtttgccc cgagttgccc
     4681 attgagccga ccacagaacc gatcgtgata atgcgtccgc cttttttcat catcgggcgt
     4741 aataccgctt tggataaacg ataaaccgaa gttaagttag tttgaatgat gtcgaaccaa
     4801 tcctcatctt tcatacgcat taataagccg tcacgggtaa tgccggcgtt attgactaac
     4861 acgtccacat cgccaaaggt ctctttgatc tgctttaata cgctctcaat cgactctgca
     4921 tcggcaacat ttagcactaa gcctttgccg ttctcgccta aataagctga aatagattcc
     4981 gccccttttt cagaggttgc ggtgccgact acgatgtagc cttttgctac caattcttcg
     5041 gcaatcgctt tgccgattcc acgggttgcc cctgtaacta atgcgatttt gctcataaat
     5101 tctccttagt atgacgttat aacgtcagaa tataaaaaat aaaaagggta atcaagtagt
     5161 ttatcacggt cgttaagcca ccaaataagg catatttcac cgcactacct tgtttagggt
     5221 aaatccaacg tgggcgagta aatgcccaaa tagctgaaaa aaacagtggc cctaaactac
     5281 taaacagata aatttgaaat aaaacgaccg cacttttggg gtcattttcg cttaatagca
     5341 caccatataa tacccaagct tgaatagcag cagctgtaat agctgaacct ccgataaagg
     5401 cgtggcgaat tttcgggttt agacttaaaa aataggctaa aaatgagcaa ccggcagcaa
     5461 tggataacat tcccatattt ccgcctaatg tttctttagc gataccaaca gtataagcta
     5521 aaggtaaaat cactaaggca atcaatgcac ttattagcat aaatagggtt tctttactca
     5581 ttttacgctc ccgaaaatta agcctcaacc gcctcaaagc tggcaacatc atttaccgct
     5641 ttggctgata attcttttac aatacgacct gctaaaccgg ttaggacttt gtttggcccg
     5701 atttcatata aagtagtgat gccatcttgt gccatttttt caacggtttc cgtccaacga
     5761 acagggctgt aaagttggcg aacaagtgca ttacgaatgg tatcggcatc tgtttccact
     5821 gccacatcca cgttgttgat aacagggaca agcggttgat ttagctcaat attttgcaag
     5881 gcttcggcta atttttctgc cgccggtttc attaaagcac agtgtgaagg tacgctcacc
     5941 gctaatggta acgcgcgttt tgcacctgcg gctttacaca attctcctgc tttttctgcc
     6001 gctgcttttg tgccggcaat cacaacttgc cccgggctgt taaagtttac cgcagagaca
     6061 atttcaccgg tctcgcttgc tgcttgctcg cacgctttaa taatgctttc gttatctaaa
     6121 ccgataatcg caaacatcgc accgctgcct gccggcacgg cttcttgcat tgcacgacca
     6181 cgcaactcga ccaatttaat cgcatcttgg aaatccaaca caccggcaca caccaacgct
     6241 gagtattcac ccaaactatg ccctgccatc acggctggtt tttgctcagg gaatttttgt
     6301 tgccaaatgc ggaaaagtgc cacgcttgag gcaagcaacg caggttgagt gcggtgggtt
     6361 tgccctaact cttcggcagt accgttttgc actaaatccc ataagtcata gccaagcaca
     6421 tcacttgctt gtttgaaggt ttcttccacg attggatatt gtggcgcaag gtcggcaagc
     6481 attccaaccg cttgtgagcc ttgaccgggg aaaaccatag cgaattttga catttgttat
     6541 tattccttct gtaaattttt aagttgaaat ctatatctga gttttcgtta ctttctttgc
     6601 ttgtgcaaag aaagtaacca aagaaacacc cctacttcac cgcttatcct cacttagttt
     6661 gaattggttt cacggaaaat ttacattcgc ttcgctcaga gtaaattttc ctaccaattc
     6721 aaacacgttc gggcggttca gaagggaaga gtacatcact atattttgta aaaaattttg
     6781 aataatgtac cgcttgtccc gtctgagcga cttgcgattt ctaggaaaat ttgtactgtt
     6841 tgagcgtagc gagtttacaa attttccgtt aaggaaatcg taactaagcg aagttcgggc
     6901 cgcctttctt ttggttactt ttctttggcg aagcaaagaa aagtaacatt taatcgggtg
     6961 agtaatagag cttaccgatt tcaatccgct gacgccctgt ttccaaccgc catttgttgc
     7021 tatctcttaa cgaatagacg caaccgcagt attcttgctg gtagaaacgt tcacgtttgc
     7081 tgatttcgat cattcgttgt gagccgccgc ctttacgcca gttgtagtcc cagtaaacca
     7141 cgtcatcaaa tttggctgct gcacggtgac cacagtcgtt gatttggttc atatctttcc
     7201 atcgggaaat gcctaagcaa ctggtaaata cagggaaacc gttgttgtga gcgtattccg
     7261 ctgctttttc aaaccgcata tcaaagcaca ttgtgcaacg aatgcctcgt tccggctccc
     7321 attccattcc tttggctttt ttgaaccact cttttcggtc gttttcgtag tcatcgtcta
     7381 aatcgataaa cggaataccc cacttttcgg caaagcgaat attctcttcc ttgcggatca
     7441 aatactcttt cagcgggtga atgttcgggt tataaaaata gatcgtaaac tcaatgccgg
     7501 aggcgtgaat agcctccatt acctcacccg agcaaggggc acagcaagag tgcagtagta
     7561 atttgttatg cccgtttggc agcgttaatt tctcacgcac aaacggggca ttcgggtctt
     7621 ttttctgatt ttgagcacgg cgtctcgctc gtacgctttc aatatgttct tggggttgtt
     7681 ggttttgttg gttttgttca gtcattgtta tgttggggca aataacattt gcccaaaaga
     7741 attataggtt atggggcaaa tatcatttgc ccctacaagt ggttaaattt atatgatttt
     7801 ttgcaaatta ataagagcga tccaccgata aatacgccaa tgaaaccaag gcttgtttgt
     7861 actcgctttc cggtaaaacc cctaatgaat ccaccgcttt ttgggcttct tcacgggctt
     7921 tttgcatggc ataatccaga gattgatgtt cttgcataat tgccaatact tgtggaattt
     7981 gttccaattc gccgccgttt tcgatagcat tgcgaatcag ctctgcttga gtag
//
LOCUS       DANKBV010000106         7945 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.106, whole genome shotgun sequence.
ACCESSION   DANKBV010000106 DANKBV010000000
VERSION     DANKBV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.106"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1933
                     /gene="pnp"
                     /locus_tag="PWY43_001873"
     CDS             <1..1933
                     /gene="pnp"
                     /locus_tag="PWY43_001873"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL2136733.1"
                     /translation="GGFFKREGRPSEGETLIARLIDRPVRPLFPEGFFNEIQVIATVV
                     SVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQFVLNPTTSEQKISRLD
                     LVVAGTDKAVLMVESEADILTEEQMLAAVVFGHEQQQVVIENLKEFVKEAGKPRWDWV
                     APEPNTDLINKVKALAETRLGDAYRIVEKQVRYEQIDAIKAEVIAQLTAEDETVSEGT
                     IIDIITALESQIVRSRIIAGEPRIDGRTVDTVRALDICTSVLPRTHGSALFTRGETQA
                     LAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRIGSPKRREIGHGRLAK
                     RGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAM
                     GLVKEDEKFVVLSDILGDEDHLGDMDFKVAGTRTGVTALQMDIKIEGITPEIMRIALN
                     QAKGARMHILGVMEQAIPAPRADISDYAPRIHTMKIDPKKIKDVIGKGGATIRALTEE
                     TNTSIDIDDDGTVKIAATDGNAAKAVMARIEEIVAEVEVNQIYNGKVTRVVDFGAFVS
                     ILGGKEGLVHISQITNERVERVADYLTVGQEVQVKVVEIDRQGRIRLTMKDINNTNEA
                     NAEETVAENVVETEQENNF"
     gene            2014..2937
                     /gene="nlpI"
                     /locus_tag="PWY43_001874"
     CDS             2014..2937
                     /gene="nlpI"
                     /locus_tag="PWY43_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDL2136734.1"
                     /translation="MVQLFKLFRIRFLLLNLISVLMLSGCTSRYADLVSIKNLALAEL
                     NPQLRFEQEAMVVRLSQVLEEAPLTPSEQADLYFERGVIYDSLGLWSLARYDFAQAIS
                     LNQRMAAAYNYMGLYLLLEGDYDSSIDAFNAVLELDREYSYTYLNRGLAFYYSGRYSE
                     AQRDLLHFYEEDKKDPYRALWLYFNELEFTPTEAKNNLQKRANNLSSDYWGTNIVNYF
                     LGKLNLAQVRAIMERDAKENSSQYAEILTETYFYLAKQQLKLDHKDEAISLFRLALAN
                     QVFNFVEYRFALFELSQIRGMTQTTPVVDKE"
     gene            3140..4945
                     /locus_tag="PWY43_001875"
     CDS             3140..4945
                     /locus_tag="PWY43_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2136735.1"
                     /translation="MTTESLTFADLGLPQPILDAVNEMGFVNPSPIQQECIPHLLAGR
                     DVLGMAQTGSGKTAAFSLPLLAQIDPTQRHPQMLVMAPTRELAIQVADACEQFAKNMK
                     GIRVVTVYGGQRYDIQLRALKQGSQVVVGTPGRILDHIRRGTLDLSELKFIVLDEADE
                     MLRMGFIDDVETVMAELPETHQTALFSATMPEPIRRITRRFMKDPQEVKIKATERSAP
                     DIEQSYWLVNGFRKNDALLRFLEVEEFDAAIIFTRTKTGTIDITELCERNGYRTAALN
                     GDMTQQAREQTLDKLRSGRLDILVATDVAARGIDIERISLVINYDIPLEEESYVHRIG
                     RTGRAGRSGRALLFVEPRERRLLRNIENLIKKPINEVAIPNHEILMEKRREKFKARIS
                     KQLEHHDLEKYRELLEDLFTADQDHEELAAAMMMLLQEKQKLILPPDPVVRAARSERG
                     RDRNERNDRRNGREHRENNGVAMDLYRIELGREDGVEVRHIVGAIANEGDISSRYIGH
                     IKLYDKYSTIELPQGMPNHLVQHFAHKARVLSKPMQMTLLGSNSASNANPFEGRGRRG
                     NDRNDRGGRRDGGFNKDRKGGFKEKRFSEKRGRRD"
     gene            5027..5551
                     /gene="tadA"
                     /locus_tag="PWY43_001876"
     CDS             5027..5551
                     /gene="tadA"
                     /locus_tag="PWY43_001876"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL2136736.1"
                     /translation="MFIKPNQTSCPLQISEQDIAFMQYALDLADLAEAKGEIPVGAVL
                     VDKNQQIIGRGWNQTIQLCDPSAHAEMQAIRQAGQTLGNYRLLDCTLYVTLEPCPMCA
                     GAILHSRIKRLVFGASDYKTGAVGSRYHLFEDYKMNHFLEVQGNVLGRECSQKISDFF
                     KQRRLQKKGDALIK"
     gene            complement(5589..6683)
                     /locus_tag="PWY43_001877"
     CDS             complement(5589..6683)
                     /locus_tag="PWY43_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HDL2136737.1"
                     /translation="MKSTIFTLPLAIITLALAGCSSDNQESYAGLNLDAKYSKSRSVS
                     HFNDYVHFLKQKAAGAGVSAKTLNAQKFIHYNARAVQLDQQQAARRRDPNVPPPPPNP
                     SGVTHYLNKVLTQAKVDQASERWYEYNSQLTHASQKYGVQKEYILALWGMESSFGRNQ
                     GDFDVLSVLATLAFDGRREKLFTQEFVNAMKMLDDGTIKRYELLGSWAGAMGQTQFMP
                     TSYLTYAADGNNDGKKDIWENEADAFASIANYLSTVGWDNQLPWGVEVKLASPMDLSF
                     AGLETNKAKTLAEWQALGIYLAYPNGQESAKLAQLHGKEVWLIRPDKEAGRAFLVSNN
                     FRTLMDWNKSNNFGISIGKFADRILASVGQ"
     gene            complement(6686..7609)
                     /gene="truB"
                     /locus_tag="PWY43_001878"
     CDS             complement(6686..7609)
                     /gene="truB"
                     /locus_tag="PWY43_001878"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2136738.1"
                     /translation="MSRPRKRGRDIHGVFLLDKPQGMSSNDILQKVKRIFQANKAGHT
                     GALDPLATGMLPICLGEATKFSQFLLDSDKRYLVTAKLGERTDTSDAEGQVVETKAVA
                     VTKSQILAALEKFRGDILQVPTMFSALKHNGKPLYEYARAGITVEREARPITIFELNF
                     IEYKAPFLTLEVHCSKGTYIRTLVDDLGEELGCGAHVTMLRRTAVADYPIEKMMSYAD
                     LQNLAENQPLAELDQHLLPMDTAVSALAKINLSSEQTKAVGFGQRIRFDNLNQLYGLV
                     RLYSEDEQFLGVAEITNDTVIRPNRMINLTE"
     gene            complement(7612..>7945)
                     /gene="rbfA"
                     /locus_tag="PWY43_001879"
     CDS             complement(7612..>7945)
                     /gene="rbfA"
                     /locus_tag="PWY43_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL2136739.1"
                     /translation="KEVAVILQREVKDPRIGMVTVSDVEVSRDLAYAKIFVTFLFDND
                     AEAIKQGMQGLEKASPYIRTLLGKAMRLRIVPELRFIYDESLVEGMRMSNLVSSVIKN
                     DEEKHKED"
BASE COUNT         2299 a         1716 c         1709 g         2221 t
ORIGIN      
        1 gggcggtttc ttcaaacgtg aaggacgtcc tagcgaaggt gaaaccttaa tcgctcgctt
       61 gatcgaccgt cctgtgcgtc cacttttccc agaaggtttc tttaacgaaa ttcaagtgat
      121 tgcgaccgta gtatcggtaa acccacaaat cagtcctgat ctggttgcga tgatcggtgc
      181 atcggctgcc ctttcattat ccggcgtgcc gtttaacggt ccaatcggtg cggctcgtgt
      241 cggttttatc aacgatcaat tcgtattaaa cccaaccacc agcgagcaaa aaatcagccg
      301 cttagattta gtggttgcag gtacagacaa agccgtgttg atggtggaat ctgaagcgga
      361 tatcttaacc gaagagcaaa tgttagcggc ggtggtgttc ggccacgagc aacaacaggt
      421 tgtaatcgaa aacctcaaag aatttgttaa agaagcgggc aaaccacgtt gggattgggt
      481 tgcaccagag ccaaatacag atttaatcaa caaagtaaaa gcattagcag aaacacgcct
      541 tggcgatgct tatcgtatcg tagaaaaaca agttcgttac gagcaaatcg atgcgattaa
      601 agcagaggtg attgcacaac ttaccgcaga agatgaaact gtttctgaag ggactatcat
      661 cgacatcatc accgcattag agagccaaat cgtgcgtagc cgtattattg caggcgaacc
      721 acgcattgac ggccgtacgg tggataccgt gcgtgcattg gatatttgca ccagtgtgtt
      781 accacgcacc cacggttctg ctcttttcac ccgtggcgaa acccaagcat tagcagtagc
      841 aacattgggc acagagcgtg atgcccaaat cattgacgaa ttgaccggcg aaaaatctga
      901 ccgtttctta ttccactaca atttccctcc atactctgtg ggcgaaaccg gtcgtatcgg
      961 ctcgccaaaa cgccgtgaaa tcggtcacgg tcgtttagca aaacgtggcg tattagccgt
     1021 gatgccaacc gctgaagagt tcccgtatgt agtgcgtgtg gtgtctgaaa tcactgaatc
     1081 taacggttct tcttcaatgg catctgtgtg tggtgcgtct cttgcgttga tggacgcagg
     1141 tgtgccaatc aaagcagcgg ttgccggtat cgcaatgggg ctcgtgaaag aagacgagaa
     1201 attcgtggta ctttctgaca tcttaggtga tgaagaccac ttaggcgata tggactttaa
     1261 agtagcggga acccgtaccg gtgtgactgc gctgcaaatg gacatcaaaa tcgaagggat
     1321 cacccctgaa attatgcgta ttgccttaaa ccaagctaaa ggtgcaagaa tgcacatttt
     1381 aggtgtaatg gaacaagcca ttccggcacc tcgtgcagat atttctgact atgccccacg
     1441 cattcacaca atgaagatcg atccgaagaa aatcaaagat gtgattggta aaggcggtgc
     1501 aacaattcgt gctttaaccg aagagaccaa tacttctatc gacattgatg atgacggtac
     1561 ggtgaaaatt gcggcaactg acggcaatgc agcgaaagca gtaatggctc gtattgaaga
     1621 gatcgttgcc gaagtggaag taaaccaaat ctacaacggt aaagtaaccc gtgtggtgga
     1681 cttcggtgca ttcgtttcca tcttaggtgg caaagaaggt ttagtccaca tttcacaaat
     1741 caccaacgaa cgtgttgagc gtgtagcgga ctacttaacc gttggtcaag aagtacaagt
     1801 gaaagtggta gaaattgacc gtcaaggacg cattcgtctg acgatgaaag acatcaataa
     1861 taccaacgag gcaaatgcag aagaaactgt agctgaaaat gtggtagaaa cagaacaaga
     1921 aaataatttc tagttcaata ataaaggcga ataacggact ttgagtgtcc gttattcagt
     1981 ttttaatttt ccttaattga gggatatgct gtaatggtac aactttttaa gttgtttcga
     2041 atccgtttct tattactgaa tttaatttct gttttaatgc taagcggctg taccagtcgg
     2101 tatgccgatt tggtttcaat taaaaactta gcattggcag aacttaatcc tcaacttcgc
     2161 tttgaacaag aggcaatggt ggtaaggctc agccaagtat tggaagaagc cccattaacg
     2221 ccctctgagc aagcagacct ctattttgaa agaggcgtta tttacgacag tttaggttta
     2281 tggtcgttag cccgttatga ttttgctcaa gccatttcgc tcaatcaacg tatggcggca
     2341 gcttataact atatggggct gtatctgctg ctggaaggcg attacgacag ttcgattgat
     2401 gcgtttaatg ccgtattgga attagatcgg gaatatagct acacctatct caatcgaggt
     2461 ttggctttct attatagcgg cagatattct gaagcccagc gtgatcttct ccatttttat
     2521 gaagaagata agaaagatcc ttatcgagca ttatggctct attttaatga gctggaattt
     2581 acacccacag aagctaaaaa taatttacaa aagcgagcta ataatctttc ttcggattac
     2641 tggggaacaa atatcgtaaa ttatttttta gggaagctca atttagctca agttcgtgct
     2701 ataatggaac gtgacgctaa ggaaaatagc tcacaatatg cagaaatact gacagaaacg
     2761 tatttctact tagcaaaaca acaactcaaa ttagatcata aagatgaggc aatcagcctc
     2821 tttcgtcttg ctcttgcaaa tcaagtgttt aacttcgttg agtatcgttt tgccctgttt
     2881 gagttatcac aaattcgtgg tatgactcaa acgactcccg ttgttgataa agaataggga
     2941 aagattagga caatacaagc ggttaatttc gcaaaaaatt ttgtaaattt aaccgcttat
     3001 actcagggga tttttaaagc atcacttgct ttgttatttc atttcttcct aaatttcaac
     3061 aatataattt tcgttaatgg ctgtgcagaa aaatagtcga tattttctgc tttgttccgt
     3121 tccaaacaaa tggtttttta tgacaactga atctttaact ttcgccgatt taggcttacc
     3181 tcaaccaatc cttgatgctg taaatgaaat gggctttgta aacccatcac ccattcaaca
     3241 agaatgtatt cctcatttat tagccggtcg tgatgtgctt ggtatggcac aaaccgggag
     3301 cggtaaaacc gccgcattct cgctgccgct gttggcacaa attgacccaa cacaacgcca
     3361 cccgcaaatg ttggtgatgg cacccacccg tgagcttgcg attcaagtag ccgatgcttg
     3421 tgagcagttt gcaaaaaata tgaaaggcat tcgtgtagtg acggtttatg gtggacaacg
     3481 ttacgacatt caattacgtg ctttaaaaca aggctcacaa gtagtagtgg gtacacctgg
     3541 gcgtattctt gaccatatcc gccgtggcac attagattta tccgaattaa aattcatcgt
     3601 gttagatgaa gccgatgaaa tgctgcgtat gggctttatt gacgatgttg aaacggtaat
     3661 ggctgaatta ccggaaacac atcaaactgc tctcttctct gccaccatgc cggagccgat
     3721 tcgccgtatt acccgtcgtt ttatgaaaga tccgcaagag gtaaaaatta aggcaaccga
     3781 gcgttcagcc ccggatattg aacaaagcta ctggctggta aacggcttcc gtaaaaacga
     3841 tgcgttattg cgtttcttag aagtagaaga atttgatgct gctatcatct ttacccgcac
     3901 caaaaccggt actattgata ttaccgaatt gtgtgaacgc aacggctacc gtactgcggc
     3961 attaaacggc gatatgaccc agcaagcccg tgagcaaacc ttagataaat tacgctccgg
     4021 tcgtttagat attttagtcg ccaccgatgt tgctgcccgt ggtattgata ttgagcgaat
     4081 cagcttggtt attaactacg atattccgtt agaagaagaa tcttatgttc accgcatcgg
     4141 acgtaccggt cgtgccggtc gttcaggtcg tgccttactg tttgttgagc ctcgtgaacg
     4201 ccgcttatta cgcaatatcg aaaatttaat caaaaaacca attaatgaag tggcgattcc
     4261 aaaccacgaa attttaatgg aaaaacgccg tgagaaattc aaagcccgca tttctaagca
     4321 gttagaacat cacgatttag aaaaatatcg tgaattgttg gaagatttat ttactgcgga
     4381 tcaagaccac gaagaattag cagcggcgat gatgatgtta ctgcaagaaa aacaaaaact
     4441 gattctgcca ccggatccgg ttgtgcgtgc tgcccgttct gaacgtggac gtgaccgcaa
     4501 tgagcgaaac gatcgtcgta acggtcgtga acaccgcgaa aacaatggtg tggcaatgga
     4561 tttataccgc attgaattgg ggcgtgaaga cggtgtggaa gttcgtcata ttgttggagc
     4621 gattgccaac gagggggata tcagcagccg ttacatcggg catatcaaac tttatgataa
     4681 atactcaacc attgagttgc cgcaaggaat gccaaatcat ctcgtacaac atttcgctca
     4741 caaagcacgt gtattaagca aaccaatgca gatgacatta cttggctcaa acagtgcaag
     4801 taatgcaaat ccatttgaag gtcgtggtcg ccgtggtaac gatcgtaatg atcgtggtgg
     4861 tcgccgagac ggtggtttca ataaagaccg caaaggtggc tttaaagaaa aacgtttcag
     4921 tgaaaaacgt ggtcgtcgtg actaaacgac actaagcgaa gtaagcgatg ccccaccctc
     4981 gtggggcatt ttacaagcgg tcaaaaaaac tcaatttttt gcaactatgt ttatcaaacc
     5041 aaatcaaact tcttgtccac ttcagatctc cgagcaagac atcgccttta tgcaatacgc
     5101 ccttgatctc gctgatttag ccgaagcaaa aggcgaaatc ccggttgggg cagtactggt
     5161 tgataaaaac caacagatta tcggcagagg ctggaaccaa accattcaac tctgtgatcc
     5221 atctgcacac gctgaaatgc aggcaattcg tcaagcagga caaactttag ggaattaccg
     5281 tctgttagat tgtacccttt atgttacgct cgagccttgc ccaatgtgtg ccggtgcaat
     5341 tttacatagt cggattaagc gattggtttt tggggcaagt gattataaaa caggtgcggt
     5401 aggttcacgc tatcatctgt ttgaagatta taaaatgaac catttcttag aagtgcaggg
     5461 caatgtattg ggcagagaat gtagccaaaa gatcagtgat ttttttaagc aacgtcgttt
     5521 gcagaagaaa ggcgacgctt tgataaaata aaagcataga taggctttgc tactatgctt
     5581 ttattcgatt attgaccaac tgaagctaaa attcgatcgg caaattttcc gatactgata
     5641 ccaaagttat tcgatttatt ccaatccatt aaagtacgga aattattaga aactaaaaac
     5701 gctcttcccg cttctttatc cggacgaatc aaccaaacct ctttgccgtg taattgagct
     5761 aattttgccg attcttgtcc attcggataa gctaaataaa tacctaaggc ttgccattcc
     5821 gccaatgttt tcgctttgtt ggtttcaaga ccggcgaaac ttaaatccat cggtgaagct
     5881 aatttaacct caacacccca aggtagttga ttatcccaac ctactgttga aagataattg
     5941 gcaatcgagg caaatgcatc ggcttcattc tcccaaatat cttttttacc gtcattattg
     6001 ccatcagctg cataggttaa gtaagaagtt ggcataaatt gagtttgtcc catcgctcct
     6061 gcccaagatc caagcaattc atagcgttta attgtgccgt catcgagcat tttcatcgca
     6121 ttcacaaatt cttgcgtaaa taacttctca cggcgaccat caaaggcgag ggttgccaac
     6181 accgaaagta catcaaaatc cccttggtta cgcccaaagc tgctctccat tccccaaagt
     6241 gccaaaatat actctttttg taccccatat ttttggctgg catgggtaag ctggctatta
     6301 tattcatacc aacgttcact tgcctgatcc accttcgctt gagttaatac tttgtttaaa
     6361 taatgggtca caccacttgg atttggtggc ggaggcggta cattcgggtc acgtcttcgt
     6421 gcagcttgct gttgatctaa ctgcaccgct ctggcgttgt aatgaatgaa tttctgagca
     6481 tttaaggttt ttgcagacac tcctgcccct gctgccttct gtttcagaaa atgcacataa
     6541 tcattaaaat gactgaccga gcgagattta ctgtatttag catctaagtt taagccggca
     6601 taactttctt gattatcact tgaacaaccg gctaaggcta aagtgataat cgctaaaggt
     6661 aaggtaaaaa ttgtcgattt cattcttatt ccgttaagtt aatcattcgg ttaggacgaa
     6721 tcacagtatc attggtgatt tctgccacac cgagaaattg ttcatcttct gaatataaac
     6781 gtaccaaacc ataaagttgg ttcagattat caaatctaat tctctgacca aaacccaccg
     6841 ctttagtttg ctctgaactt aaatttattt ttgctaatgc agaaactgcc gtatccatcg
     6901 gcaataagtg ctggtctaac tctgcaagcg gttgattttc ggctaaattt tgcaaatctg
     6961 catagctcat cattttttca atcggataat ctgccactgc tgtacggcgt agcatcgtaa
     7021 cgtgtgcacc acagcccaac tcttcgccta aatcatcgac caaagttcgg atataagtac
     7081 ctttagaaca atgcacctca agggttaaaa atggagcctt gtattcaatg aaatttagtt
     7141 caaaaatcgt aatcgggcga gcctcacgct ccaccgtaat ccctgctcgg gcatattcat
     7201 acaagggttt gccgttgtgt tttaaggccg aaaacattgt cggcacttgc aaaatatcac
     7261 ctctaaattt ctccaaagca gccaaaattt gagattttgt cactgcaact gctttggttt
     7321 ccaccacttg cccttccgca tctgaagtat cggttcgctc gcctaatttt gcggtgacga
     7381 gataacgttt atccgaatcc aacaaaaatt gcgaaaattt ggtcgcttca cctaaacaga
     7441 tcggcagcat tccggttgcg agagggtcta acgccccggt atgtccggct ttatttgctt
     7501 ggaaaattcg ctttaccttt tgcagaatat cgtttgaaga cattccttgt ggtttatcta
     7561 ataagaaaac gccgtgaata tcacggccac gtttgcgagg tctagacaca attagtcctc
     7621 tttatgtttt tcttcgtcat ttttgattac actagacaca agattagaca tacgcatacc
     7681 ttcgactagc gattcatcgt agataaaacg cagttcaggc acaatacgca aacgcattgc
     7741 tttacctaaa agggttcgaa tataggggct tgctttctcc aagccttgca taccttgctt
     7801 aatagcttca gcatcgttat caaataagaa agtcacaaaa attttggcat acgccaaatc
     7861 tcttgatact tccacatcag aaacagtcac cataccaatg cgaggatctt ttacctcacg
     7921 ttgtaaaatc accgccactt ctttt
//
LOCUS       DANKBV010000107         7942 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.107, whole genome shotgun sequence.
ACCESSION   DANKBV010000107 DANKBV010000000
VERSION     DANKBV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.107"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(205..1629)
                     /gene="lpdA"
                     /locus_tag="PWY43_001880"
     CDS             complement(205..1629)
                     /gene="lpdA"
                     /locus_tag="PWY43_001880"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL2136740.1"
                     /translation="MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGI
                     CLNVGCIPSKALLHVAKVIEEAKNAVHNGVTFGEPTIDLDQVRAGKEAVVSKLTGGLA
                     GMAKARKVTVVEGLAAFTGPNTLVARDRDGNPTTITFDNAIIAAGSRPIQLPFIPHED
                     PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKD
                     VVAIYTKQIEKKFKLMLETKVTAVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPN
                     GKLIDAGKAGVEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASEC
                     SEGMTKLIFDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNAKAKKC"
     gene            complement(1829..3733)
                     /gene="aceF"
                     /locus_tag="PWY43_001881"
     CDS             complement(1829..3733)
                     /gene="aceF"
                     /locus_tag="PWY43_001881"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL2136741.1"
                     /translation="MSKQINVPDIGSDEVSVTEVMVKVGDTISVDQSIINVEGDKASM
                     EVPAPEAGVVKEVLVKVGDKVSTGSPMLILESADAAPAQEAPKAEAAPAAPVATTSAV
                     VEVNVPDIGGDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPVAGVVKEILIN
                     VGDKVSTGKLIMKFETASAATAQSAPVAEAAPAQASASAIKDVNVPDIGGDEVNVTEI
                     MVKVGDSVSEEQSLITVEGDKASMEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAG
                     AAPAPAAAQAASAPAPQAAAPAKADAAPAGQNQSGLSQEQVVASAGYAHATPVIRRLA
                     REFGVNLDKVKGTGRKGRIVKEDIQAYVKTAVQVFEKQGGTAAAATGAANGAGLGLLP
                     WPKVDFSKFGEVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITELENFRKEQNK
                     EAEKRKLDVKITPVVFIMKAVASALEAFPRFNSSISEDAQRLTLKKYINIGIAVDTPN
                     GLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIGTTHFT
                     PIVNAPEVAILGVSKSEMQPIWNGKEFEPRLMLPLSLSFDHRVIDGADGARFLSYINS
                     VLADIRRLVM"
     gene            complement(3937..6594)
                     /gene="aceE"
                     /locus_tag="PWY43_001882"
     CDS             complement(3937..6594)
                     /gene="aceE"
                     /locus_tag="PWY43_001882"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDL2136742.1"
                     /translation="MSENLRNDVDPIETQDWLASLDSLIREEGLERAQFIIEQVISQA
                     RAGGVALPTGVTTDYVNTIPVSEQPAYPGDHKIERRIRSAVRWNAIAAVLRSQKKDLD
                     LGGHISTFQSAATMYEVCFNHFFKAPTEKDGGDLVFFQGHAAPGMYGRAFLEGRITAE
                     QMDNFRQEAFTDGLSSYPHPKLMPEFWQFSTVSMGLGPVNAIYQARFLKYLENRGLKD
                     TSAQKVYAFLGDGEMDEIESKGALSFAGREKLNNLIFTISCNLQRLDGPVNGNGKIVQ
                     ELEALFTGCGWEVIKVLWGSGWDKLLEKDTSGKLKQLMMEVLDGDYLTFKSKNGAYVR
                     EHFFGRYPETAALVADMTDDEIWALRRGAHDSEKLYAAYAKAQKSDKPVVILAHQVKG
                     YRIPEAESKNTAHQSKKMSLDSLKGFRDYFELPLTDEQVESLEYVRFPEGSEEYNYLH
                     GQRKALNGYVPVRKPKFTVDFKVPELSEFQALLDEQPRGISTTMAFSRVLNTLLKDKN
                     IGQQIVPIIADEARTFGMEGLFRQIGIYNPHGQNYVPSDRDLVAYYREAKDGQVLQEG
                     INELGATASWVAAATSYSVSNLPMIPFFIYYSMFGFQRVGDMMWLAGDQLARGFMIGG
                     TSGRTTLNGEGLQHEDGHSHIQAGVIPNCVTYDPAFAFEVAVIVQDGIRRMYGENQED
                     IFYYITTLNEITEQPAMPAGAEEGIRKGLYKFETVEGKGKGHVQLLSSGAIMRHVRAA
                     AQILANDYGITADVFSAPSFNELGRDGADVARWNLLHPTETPRVPYVAQVLQDLPTVA
                     STDYMKMYAEQIRAYVPSKHYHVLGTDGFGRSDSRANLREHFEVDERYVVVAALAQLA
                     KEGTVENKVVAEAITKFGLNVDRINPLYA"
     gene            complement(6958..7905)
                     /gene="pyrD"
                     /locus_tag="PWY43_001883"
     CDS             complement(6958..7905)
                     /gene="pyrD"
                     /locus_tag="PWY43_001883"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDL2136743.1"
                     /translation="MQALALAGKSPFSILPTPDNPTQVMGLTFKNPIGLAAGADKNGE
                     AIDGFGKLGFGFIEVGTITPLAQDGNPKPRQFRIVDAEGIINRNGFNNKGVDVLVENV
                     KNATYSGILGINIGKNAVTPIEHSFDDYQICLRKVYQYADYVAVNISSPNTKNLRSLQ
                     YGEALDDLLRNLKNEQAQLSQKMGKYRPLVLKIAPDLTDEEIVSVADSLIRYQIDGVI
                     AGNTTLSRESIAGLPYSEQLGGLSGKPLHSLSTRLISKLSAELGERIPIIGCGGVHSL
                     ESGQEKVNAGASLLQVYSAMIYQGPSLIQTLAKHLQLNS"
BASE COUNT         2231 a         1796 c         1457 g         2458 t
ORIGIN      
        1 aagtctcaca acattgcgct gtggaacgtg gtgcatttta cggatttatc attttatgtc
       61 aatgctaaat ttagcattat agattcaaat gctgaaaaaa aactcactat tcaatagttt
      121 agttttaaaa ataatcaagc ggttgtattt tgcaaaaaat ttacaaaata caaccgctta
      181 ttttttagag attaaataat ctgattaaca ctttttggct ttcgcatttg gtaagtctgt
      241 aattgaacct tcaaatactt ctgccgctaa tcctaccgat tcgtgtaacg ttgggtgagc
      301 atgaatggtt aatgcgatat cttcagcatc acagcccatt tcgattgcaa gaccgatttc
      361 gcctaataat tcgccaccat tgctaccaac aatcgcacca cctaatacac ggtgagtatc
      421 tttatcaaaa attaacttgg tcataccttc tgagcattca gaagcaatcg cacgacctga
      481 agcagcccaa gggaatttcg ccacttcgta gttcaagcct tcttgtttac actctttctc
      541 agttttacca acccaagcca cttctggttc agtataagcg atggacggaa tgactttcgg
      601 atcgaagtaa tgtttcattc ctgcaatcac ttctgctgcc acgtgtccct cgtgtacacc
      661 tttgtgagct aacatcggct ggccgacaat atcaccaata gcaaagatgt gtggcacatt
      721 ggtacgcatt tgtttatcta cacgaataaa gccacgctca tctacttcca ctccagcttt
      781 accggcatca attaatttac cgtttggcac acgtccgata gcgactaata ccgcatcata
      841 acgtttagta tcgttgcaag ccttaccttc cattgataca tagataccgt catctttagc
      901 ttctactgcg gttactttgg tttcaagcat tagcttgaat tttttctcga tttgtttggt
      961 gtaaatcgct acaacatctt tatctgcagc aggaattact tggtcgaaca tttcaaccac
     1021 ttccacttct gaaccaagcg cgttatatac cgttcccatt tctaaaccga taataccacc
     1081 acccataata aggagttttt tcggtacttc ttttaattta agagcatcgg ttgaatccca
     1141 aactcgtggg tcttcgtgtg gaataaatgg tagttgaatc ggacgagaac ctgctgcgat
     1201 aatcgcatta tcgaacgtga tagttgtagg attgccgtca cgatcacgag cgactaacgt
     1261 attcggacca gtaaatgcag ccaagccttc tactactgtc actttacgag ccttagccat
     1321 tcctgctaag ccaccggtta attttgaaac aacggcttct ttaccagcac gtacctgatc
     1381 taaatcaatc gttggctcac caaaggtcac accgttgtgt actgcatttt tagcttcttc
     1441 aattactttt gcaacgtgta ataatgcttt agaagggata caacctacgt ttaagcaaat
     1501 accacctaag gttgaataac gctcaacaat tactgtttct aagcctaagt cggcacaacg
     1561 gaatgccgct gagtaacctg ctggacctgc accaagtacg acaacttggg ttttaatttc
     1621 tttgctcata gtttttcctc tttttattcc ccccctttag caaagagggg taagggagat
     1681 ttggcagaat ataaagcgaa gtgagagagc aatcaatcaa acacaaattt ctaccaaata
     1741 atttattctc ttcgtaaatt acgtctatca aatctccccc tacccctctt ttctaaagag
     1801 ggactttttt aggaggagct ttaccgaatt acatcactaa acgacgaata tctgccaaca
     1861 cgctattaat atagcttaag aatctcgcac catcagcacc atcgatcaca cggtggtcga
     1921 atgataatga aagcggaagc attaagcgtg gttcaaactc tttgccattc caaatcggtt
     1981 gcatttcaga tttagacaca cctaaaatag ccacttccgg tgcattcacg attggcgtaa
     2041 agtgcgttgt accaataccg cctaaacttg aaattgtgaa acagccacct tgcatatcag
     2101 atgctgtcaa tttaccttca cgggcttttt tcgagacttc cattaattcg cgagaaagtt
     2161 cgataatgcc ttttttgttc acatttttga acaccggcac cactaaaccg tttggcgtat
     2221 caaccgcaat cccgatattg atgtattttt tcaaggttaa acgttgtgca tcttcagaaa
     2281 ttgagctgtt gaaacgaggg aaggcttcta atgcacttgc cactgctttc ataatgaaga
     2341 ccacaggggt aattttcacg tctaatttac gtttttccgc ttctttattt tgctctttac
     2401 ggaagttttc taactcggta atgtctgtgc gatcgaaatg tgtaacgtga ggaatcatta
     2461 cccagttacg gtgtaagttc gcaccggaaa tcttattgat acggcttaat tcaacttctt
     2521 caacttcacc aaacttgctg aaatctactt tcggccacgg taataaacct aagcctgcac
     2581 cgtttgccgc acctgttgct gccgctgctg ttccgccttg tttttcaaat acttgaacag
     2641 ccgttttaac gtatgcttga atatcttctt taacgatacg gcctttacga cctgtacctt
     2701 ttactttgtc taggtttaca ccaaactcac gagctaaacg gcgaattacc ggtgttgcgt
     2761 gtgcatagcc agctgaagcc accacttgtt cttggcttaa acctgattgg ttttgacctg
     2821 ccggagctgc gtcagctttc gccggagctg ctgcttgtgg agcaggagct gaagccgctt
     2881 gagcagccgc cggagccggt gccgcacccg ctacttcaaa tttcataatt aatgaaccgg
     2941 ttgaaacttt atcacctgat ttcactaaaa tttctttcac cacaccggca aatggagccg
     3001 gaacttccat tgaagcttta tcaccttcaa ccgtgattaa agattgttct tctgaaacag
     3061 aatcacccac tttaaccata atttcagtta cgtttacttc atcaccaccg atgtccggta
     3121 catttacatc tttaatcgct gatgccgaag cttgagctgg agcagcttcc gcaaccggtg
     3181 cagattgtgc tgtagctgcg gaagccgttt caaatttcat aatcaattta ccagttgaaa
     3241 ctttgtcacc cacattgatt aagatttctt ttactacgcc ggcaaccggt gcaggcactt
     3301 ccattgaggc tttatcacct tcaacgttga tgatagattg atccacttca acgctatcac
     3361 ctactttaac cataatttcg gttacgttaa cttcatcgcc accgatatca ggtacattta
     3421 cttcaacaac ggctgaagtt gttgctactg gagcagcagg agctgcttct gcttttggtg
     3481 cttcttgagc aggagcagca tctgctgact ctaaaattaa cattggtgaa ccggttgaaa
     3541 ctttatcacc cactttcact aaaacttctt ttaccacacc tgcttcagga gccggcactt
     3601 ccatagaggc tttatcgcct tctacgttaa taattgattg atctactgaa atagtatcac
     3661 caacttttac catcacttca gtcacactga cttcatccga accgatgtcc ggcacattaa
     3721 tttgttttga cattttgaaa tccttcttga gttaccccct ctcccattgt gggaaggaca
     3781 gatttttctt tgtaagaaga aaaatcaggg agaggggaat gcaagcggtt gattttctca
     3841 acatttttgc aaataaaact aataccctct ctgctaaaag tttctaaacg aaacctttag
     3901 ctgtctctct cccatcaagg gagagagtat tagaaattac gcatataatg ggttaatacg
     3961 atctacattt aagccgaatt tagtaatagc ttccgcaacc actttgtttt caactgtacc
     4021 ttctttcgct aattgagcga gggctgcaac tactacataa cgctcatcaa cttcgaagtg
     4081 ttcacgtaag tttgcacggc tgtctgaacg accgaaaccg tctgtaccta acacgtgata
     4141 gtgtttactt ggtacataag cacggatttg ctctgcgtac attttcatat agtcggttga
     4201 agcaacagtt ggtaaatctt gtaacacttg agcaacgtac ggtacacgtg gtgtttcagt
     4261 tgggtgtaat aagttccaac gagccacatc tgctccatca cgacctaatt cattgaatga
     4321 tggtgctgag aatacatctg ctgtaatgcc gtagtcattc gcgagaattt gtgcagctgc
     4381 acgcacgtga cgcataattg cacctgagct taataattgt acgtgacctt tacctttacc
     4441 ctcaacagtt tcaaatttat ataaaccttt acggatacct tcttccgcac ctgccggcat
     4501 tgccggctgt tcggtaattt catttaaggt agtaatgtag tagaagatat cttcttggtt
     4561 ttcaccatac atacgacgaa taccgtcttg aacaataacc gcaacctcaa aggcaaatgc
     4621 agggtcataa gtgacgcagt tcgggattac acctgcttga atatggctat gaccatcttc
     4681 gtgttgtaaa ccttcaccgt ttaatgttgt acgacctgaa gtaccaccaa tcataaagcc
     4741 acgagctaat tggtcaccgg ctaaccacat catatcgcct acacgttgga aaccaaacat
     4801 tgagtagtag atgaagaatg ggatcatcgg taagttgcta acagaataag aagtcgctgc
     4861 tgccacccaa gatgctgttg cacctaactc attgatacct tcttgtaaca cttggccatc
     4921 ttttgcttca cggtagtaag ctactaaatc acggtctgac ggtacatagt tttgaccatg
     4981 tgggttataa ataccgattt gacggaataa accttccata ccgaaagtac gggcttcgtc
     5041 cgcaataata ggtacgattt gctgaccgat atttttatct ttcaataaag tattcaatac
     5101 acgagagaac gccattgtgg ttgaaatacc acgtggttgc tcatccaata atgcttggaa
     5161 ttcgcttaat tcaggcactt tgaaatcaac cgtaaatttc ggtttacgta caggcacata
     5221 accgtttaag gctttacgtt gtccgtgtaa gtagttgtac tcttcagaac cttcagggaa
     5281 tcttacatac tctaggcttt ctacttgctc atcggttaat ggtagctcga agtagtcacg
     5341 gaagcctttt aagctatcta atgacatttt tttcgattgg tgagcagtat ttttactttc
     5401 cgcttcagga atacggtagc ctttcacttg gtgagctaaa attacaaccg gtttgtccga
     5461 tttttgtgct tttgcatagg ctgcataaag tttttcagaa tcgtgtgcac cacggcgtaa
     5521 tgcccaaatt tcatcgtctg tcatatcagc aactaatgct gcagtttcag ggtaacgacc
     5581 gaagaagtgt tcacgaacgt aagcgccatt tttggatttg aaggttaaat agtcaccatc
     5641 aagcacttcc atcattaact gtttaagttt gcctgaagta tctttttcaa gcagtttatc
     5701 ccaaccacta ccccataaca ctttaatcac ttcccagcca caaccggtaa agagtgcttc
     5761 taattcttgc acgattttgc cgttaccgtt aaccggaccg tctaaacgtt gtaagttaca
     5821 gctaatagtg aagattaagt tatttaattt ttcacgaccg gcgaatgata atgcaccttt
     5881 agattcgatt tcatccattt cgccatcgcc taagaatgca tatacttttt gagccgaagt
     5941 atcttttagg ccacggtttt ctaaatattt taagaaacga gcttggtaaa tagcatttac
     6001 cggtcctaaa cccatagata cggtagagaa ttgccaaaat tcaggcatta atttagggtg
     6061 tgggtaagaa gataaaccgt ctgtaaatgc ttcttgacgg aagttatcca tttgttctgc
     6121 ggtaatacga ccttctaaga acgcacgacc atacataccc ggtgctgcgt ggccttggaa
     6181 gaacactaaa tcaccaccat ctttttcggt tggtgctttg aaaaagtggt taaaacacac
     6241 ttcatacata gttgccgcag attggaaagt tgaaatatga ccacctaagt caagatcttt
     6301 cttctggcta cgaagtactg ccgcaatcgc attccaacgt accgcagaac gaatacgacg
     6361 ttcgatttta tgatcacccg gataagctgg ctgctcggaa acagggatag tattgacata
     6421 atctgttgtt acgcctgttg gtaatgccac gccacctgca cgagcttggc taataacttg
     6481 ttcaatgata aattgagcgc gctctaaacc ttcttcacga attaatgaat cgagtgatgc
     6541 taaccaatcc tgagtttcga ttggatctac gtcatttctt aagttctctg acatagtatt
     6601 cttccttata aattaacgat tgaggtgata aataacaaaa ttttaacaaa ttttgtttgt
     6661 ttggtgattt ttagaccaat tcttatcgaa aaaatttcag taaaacaaca atttccgaat
     6721 taaacctctc ttgaggcata tactaaaaat catgattaaa acttattatc tatttgattt
     6781 taaacgattt ttatataatt ttataaaatt tgtttaaatt ttgtagataa atttgcagtg
     6841 caagatagca aaaataggca attttgtcta tttttgatag atagctaata ccgccatcta
     6901 ttccactaag atggcagaaa taaacctaac caatcatttt taactagaaa tctatcccta
     6961 actgttcagc tgaaggtgtt tggctaaggt ttgaattaat gatggtcctt ggtaaatcat
     7021 tgcggaatag acttgcaata aactagcacc tgcattcact ttctcctgcc cactctctaa
     7081 cgaatggact cccccacaac ctataattgg tattctctcg cccaactcgg cagataattt
     7141 tgaaattaac cttgtactta atgagtgtaa tggcttgcca cttaatccac ccagttgttc
     7201 cgaatacggt aatccggcaa tactttcacg agacaaggta gtattgccgg caatcacccc
     7261 atcaatttgg taacgaataa gactatcggc tacagaaacg atttcttcat cggttaaatc
     7321 aggcgcaatc tttaaaacaa gcggtcgata tttccccatt ttttgcgaaa gttgtgcctg
     7381 ctcatttttg aggtttcgca gcaaatcatc taatgcctca ccatattgta aactgcgcaa
     7441 atttttggta ttgggtgatg aaatattcac cgccacataa tcagcatatt ggtaaacctt
     7501 acgcaaacaa atttggtagt catcaaagct atgctcaatt ggtgtaaccg cattttttcc
     7561 aatattaata cctaaaatac cactataggt tgcatttttt acattttcta ctaaaacatc
     7621 aacgccttta ttattaaatc cattgcggtt aataattccc tctgcatcta caatacgaaa
     7681 ttgcctcggt ttagggtttc cgtcttgagc gagaggagta atagtcccaa cctcaataaa
     7741 cccaaatcct aatttcccaa aaccatctat cgcttcaccg tttttatccg ctccggctgc
     7801 aagccctatt ggatttttaa acgtgagtcc cattacttgg gtcggattat cgggtgttgg
     7861 cagaatcgaa aaaggcgatt ttccggctaa agcaagtgcc tgaatagaga attgatgtgc
     7921 tttttccgta tctagtgcaa aa
//
LOCUS       DANKBV010000108         7917 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.108, whole genome shotgun sequence.
ACCESSION   DANKBV010000108 DANKBV010000000
VERSION     DANKBV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.108"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(85..1251)
                     /locus_tag="PWY43_001884"
     CDS             complement(85..1251)
                     /locus_tag="PWY43_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2136744.1"
                     /translation="MTNLLLAIIYLSFISLGLPDAILGSAWPSMHQQLAVPLSYAGVV
                     SMIMSIGTITSSLMSDKLTYHLGTGKVMVISVALTALALWGFSISDAFWILCLWAIPY
                     GLGAGAVDAALNNYVAVHYASRHMSWLHCMWGVGASIGPHILGYALLQGRGWEAGYFT
                     ISMLQIGLTLMLFCSLPFWKLRKQITAEINHSAKPMSLKETLKIPGVKYGILAFFGYC
                     GVEQTAGLWASSYFVMVKDVGLEQAAAFGSIFFIGVTVGRGVSGFLTLKFSNRQMIKL
                     GSALILVGIAILCLPLGKYVTLASLIIIGLGCAPIFPCLIHSTPAFFGEDKSQSVIGV
                     QMASAYIGVLLLPPIFGFIANAISIQLLPLYLLLMFAIMAYAYRNLVRETSPSP"
     gene            complement(1403..2917)
                     /gene="putP"
                     /locus_tag="PWY43_001885"
     CDS             complement(1403..2917)
                     /gene="putP"
                     /locus_tag="PWY43_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDL2136745.1"
                     /translation="MFGLDPTTIMFVVYILGMIAIGFIAYRVTNNLSDYILGGRRLGS
                     FVTALSAGASDMSGWLLMGLPGAVYAGGLIEGWIAIGLTIGAYLNWKLVAGRLRSHTE
                     HSGDALTLPEYFHNRFGDKTRLIKILSAVIFLLFFAIYCASGVVAGARVFENLFGLPY
                     HQAIWYGAVATILYTFIGGFLAVSWTDTIQASLMVFALILTPFFVMYNLGGFDEMQAL
                     IDRAGEMAQKDYNDIFTGTSVLGIISLMAWGLGYFGQPHIVVRFMAAESVKSLENARR
                     ISMTWMVICLLGAIGIGYFGMAYFFGHPEQVGNISKNPEQIFIELAKLLFNPWVAGIL
                     LSAILAAVMSTLSAQLLICSSAITEDLYKGILRPQASDKELVWLSRLMVLAVAALAIY
                     IAQDPDSKVFGLVRNAWAGFGSAFGPVVLLSLFWKRMNGFGAIAGMLTGALIVHFWGD
                     ITLKLALPEIYSMIPGFIAATLMIIVVSLITPMPSKAVLDTFDRANKAYLAEVK"
     gene            complement(3011..3799)
                     /locus_tag="PWY43_001886"
     CDS             complement(3011..3799)
                     /locus_tag="PWY43_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL2136746.1"
                     /translation="MNAKKEKESSGNQSLIRGLTLLELLAKFPNGCPLAHLAELSGLN
                     KSTVHRLLQGLQQEGYVRPAPTAGSYRLTTKCLAIGQRALSSINILHIAAPHLEALNL
                     TLGETVNFAMRENDHAILINKLEATTGMMRTRAYIGQQMQLYCSGMGKLFLAYGSADY
                     IPQYWQTKSDIIQKLTVNTITTIDGMQTELETIRRQGFAMDNEENELGVSCIACPIFD
                     HQQKVHYAISVSLSTARLNQLGRENLLPAIQETAEKISAELGGR"
     gene            complement(3796..4374)
                     /locus_tag="PWY43_001887"
     CDS             complement(3796..4374)
                     /locus_tag="PWY43_001887"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL2136747.1"
                     /translation="MNQSSLTDLRSQLRQSMRLKRQSLTAGQQTQAAESIIPQALSLI
                     ESYQASHIAFYLPFNGEISPIPLMEQLLQQGKKLYLPLLHPFTSGQLLFVNYNCKTIL
                     KFNRLGIQEPILDVRNILPLQELEMIFTPLVACDKAGNRLGMGGGFYDRTLAQAPHLI
                     SVGLAHECQQVEQLPIESWDMPLDHIILGAAK"
     gene            complement(4376..4567)
                     /gene="ssrS"
                     /locus_tag="PWY43_001888"
     ncRNA           complement(4376..4567)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWY43_001888"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4672..4962)
                     /locus_tag="PWY43_001889"
     CDS             complement(4672..4962)
                     /locus_tag="PWY43_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL2136748.1"
                     /translation="MSANNIELQIFGQVLRLQCPVEQQEHLLASAQRLEERVAVLKEQ
                     SGIIQLEKVLAIVALNLNYELEQAQRQNAENKNVLVACIEQLDNSLGKFQSN"
     gene            5041..5598
                     /locus_tag="PWY43_001890"
     CDS             5041..5598
                     /locus_tag="PWY43_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HDL2136749.1"
                     /translation="MAITDSQQFKQLVSQLQIDDTPAEFHGFLCGLIAGGIQDESWKT
                     LTYQFTNDGHAFSIEPLNTLTDFYQQLSESFSEANTLFSLWLPQNEEDGFALADGISE
                     WTNSFLLGLGVAQPKLQQETDEVGEAIDDLDEIAKLGYSDDDNNEELLEAGEEVLEYL
                     RVLALFLHSHFALGVPKTEEKPPLH"
     gene            5696..6760
                     /gene="hemE"
                     /locus_tag="PWY43_001891"
     CDS             5696..6760
                     /gene="hemE"
                     /locus_tag="PWY43_001891"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDL2136750.1"
                     /translation="MSELKNDRYLKALLREPVDMTPVWMMRQAGRYLPEYKATRAEAG
                     DFMSLCRNADLACEVTLQPLRRYELDAAILFSDILTVPDAMGLGLSFGAGEGPKFARP
                     VASLNDVNQLPIPDPEDELQYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTKIKKMLYAEPHILHKLLDKLADSVILYLNAQIKAGAQAVMVFDTWGGVLAHED
                     YKAFSLHYMHKIVDGLIRENEGRKVPVTLFTKGGGLWLEAIAETGCDAVGLDWTVDIA
                     DARRRIGDKVALQGNMDPSVLYASPERIEQEVKNILAGFGNGSGHVFNLGHGIHLDVP
                     VESPKVFVDAIHNYSKQYHK"
     gene            6907..7497
                     /locus_tag="PWY43_001892"
     CDS             6907..7497
                     /locus_tag="PWY43_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HDL2136751.1"
                     /translation="MRNPIHKRMEHFEPWQNTTFMACLCERMYPNYQLFCEVTEQPEK
                     AKVFQNILNLVWEYLTIKGAKINFDNQLEKLEEIIPDVNEYAFFGVLPAQEACEALSE
                     LLHSIIAGSTLEQAIRISQISLGTVASYLEMQQDKELNEQELKNSEEIQEELDVQWQI
                     YRLLNECEKRDLDLIFELKDEIRSSGISNIGLNINQ"
     gene            7633..7905
                     /locus_tag="PWY43_001893"
     CDS             7633..7905
                     /locus_tag="PWY43_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL2136752.1"
                     /translation="MNKTELIDAIAAGANLTKKDAKAALEATLDAISASLKAGDAVQL
                     IGFGTFKVNERKARTGRNPKTGEEIKIAAAKVPAFVSGKALKDLVK"
BASE COUNT         2476 a         1721 c         1644 g         2076 t
ORIGIN      
        1 aaggggagag agctaaatta ttcgcttgtc gacccctctc cactcgtggg agagggacag
       61 attcttcttc gttcagaaga aaaatcaggg agagggggaa gtttctcgca ctaaattccg
      121 ataagcatac gccataatcg caaacatcaa cagcaagtaa agcggtaata attgaatgct
      181 gatagcatta gcaataaagc cgaaaatcgg tggcaataac aggacgccga tataggcact
      241 tgccatttgc acgccgatca ccgattgaga tttgtcctcg ccgaaaaacg caggggtcga
      301 atgaattaag cacgggaaaa tcggggcaca acctagcccg ataataatta aacttgccag
      361 cgtaacatat ttgcctaacg gcaagcacag tatcgcaatg ccgactaaga tcaaggctga
      421 accaagttta atcatttgtc tgttgctaaa tttaagcgtc aagaagccac ttacgcctct
      481 gccaacggta acgccgataa agaaaatact gccgaacgca gccgcttgtt ctaagccgac
      541 atctttaacc attacaaaat agctactcgc ccacagtccg gcggtttgtt ctacgccaca
      601 atagccaaaa aaagctaaaa tgccatattt cacgcccgga atttttagcg tttcttttaa
      661 gctcatcggt tttgccgaat ggttgatttc agcggtaatc tgcttgcgta atttccaaaa
      721 aggcagtgag caaaacagca ttagcgttag cccgatttgt aacattgaaa tggtgaaata
      781 gcctgcctcc cagcctcgcc cttgcaataa ggcgtagcct aaaatgtgcg gcccgataga
      841 agctccaacg ccccacatac aatgcagcca gctcatatgg cggcttgcgt agtgaactgc
      901 tacatagtta ttcaacgccg catcaaccgc tccagcccct aagccgtaag gaattgccca
      961 taaacataag atccagaaag catcactgat tgaaaagccc cacaatgcta aagccgttaa
     1021 ggcaacgctg atgaccatta ctttgcctgt gccgagatgg taggtcagtt tgtccgacat
     1081 taagctagag gtaatagtgc caatcgacat aatcatcgag accacaccgg cataggaaag
     1141 cggaacagca agctgttggt gcattgacgg ccacgccgag cctaaaattg catcaggcaa
     1201 cccaaggctg ataaaagaga gataaataat agcgagtagg aggttagtca tagcttacaa
     1261 tcggtcacaa aaagcatcgc tttttgaacg ttggctctgc caacgggagc gaagctgtga
     1321 acaatcctta caaggattgt gaataatttt tgataaattt tttgtaaaaa aatggcttaa
     1381 ggagacctta agccaaattt tattatttca cttctgccaa ataggcctta ttagcacggt
     1441 caaaggtgtc taatacggct ttgctcggca ttggcgtgat aagtgatacc acaatgatca
     1501 ttagggtagc agcaataaag cccgggatca tcgagtaaat ttccggtaag gcgagcttaa
     1561 gtgtaatatc gccccaaaaa tgcacgatta acgcaccggt cagcattccg gcaatcgccc
     1621 caaaaccgtt catacgtttc cagaatagtg aaagcaatac cacaggccca aatgccgagc
     1681 cgaaacctgc ccacgcatta cgcactaagc caaatacttt gctgtccggg tcttgggcaa
     1741 tataaatagc taatgctgcc accgcaagca ccatcaagcg gcttaaccaa accagttctt
     1801 tatcggaggc ttgagggcgt aaaatgcctt tgtataaatc ttcggtaatc gcacttgaac
     1861 aaatcagtaa ttgagcggaa agggtactca ttaccgcagc aagaatggca gataataaaa
     1921 tgccggctac ccaagggtta aataacaatt tagctaattc aatgaaaatt tgttccggat
     1981 ttttgctgat attgccgact tgctccgggt gtccgaagaa atatgccata ccaaaataac
     2041 caataccgat tgcacctaat aagcaaatca ccatccaagt catactgata cggcgtgcat
     2101 tttcaagaga cttcacactt tctgccgcca taaaacgcac cacaatatgc ggttgtccga
     2161 aataacccag cccccacgcc attaagctga taatgcctaa tacgctggtg ccggtgaaaa
     2221 tgtcgttgta gtctttttgt gccatctcgc ctgcacgatc aattaaggct tgcatttcat
     2281 caaaaccacc taagttatac atcacaaaaa acggggtaag aatcagggca aaaaccatta
     2341 atgaagcttg aatagtatcc gtccaactta ctgctaagaa accaccaata aaggtgtaaa
     2401 gaatggtagc aacagcccca taccaaattg cttggtggta aggtaagcca aataagtttt
     2461 caaatactct cgcaccggcc acgacgcccg atgcacagta aattgcaaaa aagagtaaaa
     2521 aaatgaccgc tgataaaatc ttaattaaac gggttttatc gccaaaacga ttgtggaagt
     2581 attcgggtaa ggttaaggca tcgccactgt gttccgtgtg agagcgtaat ctgcctgcga
     2641 ctaatttcca gtttaaataa gcaccgatag ttaaaccgat agcaatccag ccttctatca
     2701 aaccgcctgc ataaacggct ccgggtaatc ccatcagcaa ccagccggac atatctgatg
     2761 caccggcaga aagtgcggtc acaaagctac ctaaacggcg accgcctaaa atatagtctg
     2821 aaaggttatt ggtaacacga taggcaataa agccgatggc aatcattcct agaatataga
     2881 caacaaacat aattgttgtt ggatcaagac caaacattat taatgaatcc tcaagatttc
     2941 ttattgtata aagattaaag tgagtaaagg gtaaaagatt acctgaaata atcaaaatgg
     3001 caacacaaaa ttaccgtccg cccagctcag ctgaaatttt ttctgctgtt tcttgaatag
     3061 ccggtagtag attttcccgc cccagctgat ttaaacgagc agttgaaagc gaaacagaaa
     3121 tcgcataatg gactttttgt tggtgatcaa aaatcgggca agcaatacaa gaaaccccta
     3181 attcattttc ttcgttatcc attgcaaaac cttgacggcg aatggtttcc agctcggttt
     3241 gcattccgtc aatcgtggtg atggtattga ccgtcagttt ttgaatgatg tccgatttag
     3301 tctgccaata ttgtggaatg taatctgccg aaccataagc aagaaacagt tttcccattc
     3361 ccgagcaata gagttgcatt tgttgcccga tataggctcg ggtacgcatc attccggtgg
     3421 tagcttctaa tttattgatt aaaatagcgt gatcgttttc ccgcatcgca aaatttaccg
     3481 tctcgcctaa ggttaaattt agggcttcta aatgtggggc ggcaatatgc aaaatgttaa
     3541 tcgaacttaa ggctcgctgc ccgattgcca aacatttggt ggttaaacga taactgcccg
     3601 cagtcggtgc agggcggacg tagccctctt gttgtaagcc ttgtagcaga cggtgaaccg
     3661 tgcttttgtt cagccccgaa agctcggcta aatgggcaag cggacagccg tttgggaatt
     3721 tcgctaacaa ttccaatagt gttaagccac gaattaaact ttgatttcca ctcgattcct
     3781 tttctttctt tgcattcatt tggctgctcc caaaataata tgatctaacg gcatatccca
     3841 actctcaatc ggtaattgtt cgacttgctg acattcgtgt gccaaaccga cactaatcag
     3901 atgaggtgct tgagcaaggg ttctatcata aaagccacct cccattccca gccgatttcc
     3961 ggctttatca caagcaacaa gtggggtaaa aatcatctct aattcttgca acggaaggat
     4021 atttctaaca tctaatatcg gctcttgaat ccccaagcgg ttaaatttta gaatagtttt
     4081 gcaattgtaa tttacaaaca ataattgacc gcttgtaaaa gggtgcaaca gcggcagata
     4141 cagctttttg ccctgttgaa gtaattgttc cattaaagga ataggagaaa tttcaccgtt
     4201 aaaaggcaaa taaaaggcaa tatgtgaggc ttggtaagat tcaatcaatg aaagagcttg
     4261 aggaataatg gattcggctg cttgagtttg ctgaccggca gttaaacttt gccgtttaag
     4321 ccgcattgat tgccgtaatt ggctacgaag atcggttaaa gatgattgat tcatataaag
     4381 acccgagatg ccgctaccag taatggtcct tgaaccagtc ggttcaaggg agtcaaactt
     4441 aactgctttt aggcttctta ctcctaatta aattagggta agcttgctca ccagcgttag
     4501 atgtccaact caaagtttca aattatcggc tcagggacgt aactcgtaac gcacacccca
     4561 ggcaagactt atagtaacgg aatttgatga tttttcctag tatttaatcc cacaaaatgc
     4621 aaattctcaa tctttttggc tctattccta atcaattcga ttaaaaatta cctaattgga
     4681 ttgaaatttg ccaagcgaat tatctaactg ctcaatacaa gccaccaaca catttttatt
     4741 ttctgcattt tggcgttggg cttgctccag ttcataattt aaattcagtg ccacaatcgc
     4801 taacactttt tcaagctgaa taatgccgga ctgctctttc aataccgcca cacgctcttc
     4861 caaacgctgt gctgaagcca gtaaatgctc ttgctgttca acagggcatt gcaagcgtaa
     4921 aacctgccca aaaatctgta gttcgatatt atttgccgac ataaatacga aatcctcact
     4981 taatcgcaaa atttttgcta tattatgcca ttctttagca aaatcacaaa taatcccatt
     5041 atggctatca cagattccca acaatttaaa caactggtat cacaattaca aattgatgat
     5101 acaccggcag agtttcacgg ttttttatgc ggtttaattg ccggcggtat tcaagatgag
     5161 tcttggaaaa ccctcaccta ccaatttacc aatgacggac acgcattttc catcgagccg
     5221 ttaaacactt taaccgattt ttatcagcaa ttaagtgaaa gcttttcaga agccaacacg
     5281 ctgtttagcc tttggttgcc acaaaacgaa gaagatggtt ttgccttagc tgatggtatc
     5341 tccgaatgga caaacagttt cttacttgga cttggcgttg ctcaacctaa attacaacaa
     5401 gaaaccgatg aggttggcga ggcaatcgat gatttagatg aaatcgccaa actaggctac
     5461 agtgacgatg ataataacga ggaactactg gaggctggcg aagaagtgtt ggaatatctg
     5521 cgagttttag ccttattctt gcacagccat tttgctttag gcgtgccgaa aaccgaagaa
     5581 aagccaccat tacactaatg aataaataca agcggttcaa tttcatctct attttgcaaa
     5641 tatattatgc ggggcgaaaa atatttcgcc cctaccaaaa tcaaaggaaa aaccaatgtc
     5701 agaattaaaa aacgatcgct atttaaaagc gttattgcgt gaacctgtgg atatgacccc
     5761 ggtctggatg atgcgtcaag ccgggcgtta tttgccggaa tataaagcca cccgagcaga
     5821 agccggcgat tttatgtcac tctgccgtaa cgctgattta gcgtgtgaag tcaccttaca
     5881 accgctacgc cgttatgaac tcgatgcagc tatcctattt tccgatattt taactgtgcc
     5941 tgatgcaatg ggattaggtt taagttttgg tgcaggtgaa ggccctaaat ttgcccgccc
     6001 ggtggcaagc ctcaatgatg tcaatcagct accgattcct gatccggaag acgagttgca
     6061 atatgtaatg aatgcggtgc gtaccattcg ccgagaattg aaaggcgaag tgccactaat
     6121 tggtttttct ggcagccctt ggacactcgc cacctatatg gtggaaggtg gttcaagcaa
     6181 agcatttacc aaaatcaaaa aaatgcttta cgctgagcct cacattctac acaaattgct
     6241 ggacaagtta gctgacagtg tcatcttata cttaaatgcc caaatcaaag ccggtgctca
     6301 agcggtgatg gtattcgaca cttggggcgg tgtattggct cacgaggatt acaaagcctt
     6361 ctcattacac tatatgcaca aaattgtgga cggcttaatt cgagaaaatg aaggacgtaa
     6421 agtgcctgtt accctattca ccaaaggcgg aggcttatgg ttagaagcga ttgcagaaac
     6481 gggctgcgat gccgttgggc tagattggac agtcgatatt gccgatgctc gccgccgtat
     6541 tggcgacaaa gttgcgttgc aaggcaatat ggatccaagc gtgctgtatg caagccctga
     6601 acgaatcgaa caagaagtaa aaaacattct tgccggcttc ggcaacggca gcggacacgt
     6661 ctttaatctt ggacacggca ttcatttaga tgtgccggtg gaaagcccga aagtgtttgt
     6721 tgatgcgatt cacaactact cgaagcagta tcataaataa tggataaaat gccctctctc
     6781 cttgtgggag agggacagat ttttctttgt tcagaagaaa aatcagggag aggggcaagc
     6841 ggtcattttt atacagaatt ttgcaaaatc gttcccttct ctctgaaaaa taaagaagga
     6901 aatacaatga gaaacccgat tcataaacgc atggaacatt tcgagccttg gcaaaacacc
     6961 acatttatgg cgtgcctatg cgaaagaatg taccccaact atcagctttt ttgtgaagta
     7021 accgaacagc ctgaaaaagc taaggtattc caaaatattt taaatttagt ctgggaatat
     7081 ttaaccataa aaggggcaaa aattaatttc gataaccaac tggaaaaatt agaagaaatc
     7141 attcctgatg tgaacgaata tgcgtttttt ggtgtcttac ctgcacagga agcttgcgaa
     7201 gccctttctg aacttcttca cagtattatc gctggttcaa cccttgaaca agcaatccgc
     7261 atcagccaaa tttcattagg cacagttgcc agctatttgg aaatgcaaca agataaagaa
     7321 ctgaacgagc aagagctaaa gaattctgaa gaaattcaag aagaactgga cgtacagtgg
     7381 caaatatacc gcttgctaaa cgaatgtgaa aaacgtgatc ttgatctcat ttttgaactt
     7441 aaagatgaaa tcagaagcag cgggattagt aatatcgggt taaatattaa ccaatagcat
     7501 tgatatttgc aaaatcttgc ttaataatac tttgagaaat cttaactttg aattaagcta
     7561 tcgcaaggtt tcaataactt ctcagacgtt ttacgtcatt tattaaaaac acatatctta
     7621 gaggataaaa ctatgaacaa aactgagtta atcgatgcta tcgccgctgg tgcaaactta
     7681 accaagaaag atgctaaagc agcattagaa gcaactcttg atgcaatctc tgcaagctta
     7741 aaagcaggcg acgctgttca attaatcggc ttcggtactt ttaaagtaaa tgagcgtaaa
     7801 gcacgtactg gtcgtaaccc aaaaactggt gaagagatca aaattgcagc agctaaagta
     7861 cctgcattcg tttcaggtaa agcattaaaa gatttagtaa aataattctt taaagat
//
LOCUS       DANKBV010000109         7794 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.109, whole genome shotgun sequence.
ACCESSION   DANKBV010000109 DANKBV010000000
VERSION     DANKBV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.109"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..485
                     /locus_tag="PWY43_001894"
     CDS             <1..485
                     /locus_tag="PWY43_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF817 family protein"
                     /protein_id="HDL2136753.1"
                     /translation="KLFNVPLFTGFMYAAVGSYIIQAWRLFDLKIKSHLPYWLATLTA
                     LMIYLNFFTHHYIGDYRWYIAAFALGLYARTFVFFTPYDITRKMPLLLSFFLIGFFIW
                     LAENIGTFAGIWRYPNQFGAWSMVHMSKWSAWALLVMMTFTIIVHLKHIKQTVRVSSD
                     "
     gene            complement(571..1656)
                     /locus_tag="PWY43_001895"
     CDS             complement(571..1656)
                     /locus_tag="PWY43_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2136754.1"
                     /translation="MEYRYYITTALVIAVLQLFLFNFAKTVSWLFNLSSKNRKTVGIL
                     TFLVANGLVILHLTRTFALFRIMAFILVLLLFAFFISFFTACIYKIFNGNTTVNRWLK
                     IAYPFAFFGLLGLGYYNAYTPTVVHYSVKLDKPLEKPLRIGMASDLHLGKLFGTKQLD
                     QLAEIFNQQKVDLILLPGDIMDDNTEVYVADKMQPHLAKLKAPLGVYATLGNHDFFGA
                     QETIAKEITDAGITVLWDQTAEINGKFTLIGRNDDLVKNRPSAEQLLQNVNTKLPVIL
                     LDHRPTEIEQHAKLPIDIQVSGHAHKGQVFPASIITKLIYRLHYGYEKIGFGHYFVTS
                     GYGFWGVPMRLGSQSEVMIIDVEGENP"
     gene            complement(1678..2658)
                     /locus_tag="PWY43_001896"
     CDS             complement(1678..2658)
                     /locus_tag="PWY43_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL2136755.1"
                     /translation="MLFDSIQFSIGVTLPTILLMLFGIFPRRRKFIDDTFCNMASKVV
                     FNFTLPPMIFLNMLKSPLDFSSQINLISTGVGGSILIYLWAEWWAARHIENRRYRCIF
                     TQGTFRTNAAILGIALILNAYGDKALAAASVYISCLVITFNVLAVITLTSSLSEKKVN
                     FGRLILSILRNPLILAVIAGLTFNYFELSHQIPTPLESAGKLMASLTLPLALICTGAS
                     INFKQLKQFNQGVESTINKIVLFSASIRLIFAPIFLLLLGKFVFQLPPMELGIVFVAA
                     SAPVASATYAMTRNYGGDGEAAANLIGITTLGSMFSASIGLFLLRQIGWV"
     gene            complement(2761..3859)
                     /gene="prfB"
                     /locus_tag="PWY43_001897"
     CDS             complement(join(2761..3783,3785..3859))
                     /gene="prfB"
                     /locus_tag="PWY43_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550889.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2136756.1"
                     /translation="MFELNPIKNQLTDLSERTAVIRGYLDFEHKVERLEEVNAELEQP
                     DVWNKPEKAQALGKERAMLEQIVNTIKALEQGIEDVEGLIELAVEAEDQDTFNEAQVE
                     ADELEKKLADLEFKRMFSGPHDSADCYLDLQAGSGGTEAQDWTEMLLRMYLRWAESKG
                     FKAELMEASDGDVAGIKSATVKISGDYAYGWFRTETGIHRLVRKSPFDANNGRHTSFS
                     AAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQN
                     GRSQHQNKDQAMKQLKAKLYELEMSKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DHRTGVEHRTTQQVLDGDLDKFVEASLKAGL"
     gene            4115..5077
                     /locus_tag="PWY43_001898"
     CDS             4115..5077
                     /locus_tag="PWY43_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDL2136757.1"
                     /translation="MAKRPSDIDATLLQLEILRRIPKLPAKIDAKSLHQQLLDAGFER
                     DIRTVQRSLKMLCEHFDIECDERSKPFGYSWKIRSEGLALPILNEQQSLLLKLAEQQL
                     KYLLPANLMASMKPFFEQADRMVSGAKDKPEYQWLNKVCSTPTSLPLIPAKIKEEIFN
                     SVSQALFQNKLLKIEYQNQHGKKHQATIMPLAISQQGASIYLVCRYEGFDDNRLLALH
                     RIRKAEISTFSFERPKDFNLQAYQDAGHLGFGNGEKASLTFSISRDAGFHLTETPLAK
                     DQKILEESETHYRIQAIVADNDMLTWWLRKFGEDIWDIEKEALK"
     gene            5324..5965
                     /locus_tag="PWY43_001899"
     CDS             5324..5965
                     /locus_tag="PWY43_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDL2136758.1"
                     /translation="MPFQLTFCQQAGNQHQKNQDALFNGKAVYQWQLKNAESYLLDND
                     KVIFGVADGVSSHPRNHLASHYLMALLSQCQSLDSQWLRQAHGDFCEKLAKDYFGASC
                     TFVACELHHNGRGRMVNVGDSRAYKITANGEWQQLSFDHTILAEMKQKGLVKEGSNYA
                     SMYNGLTDCIVADFDADDFRIHIAEFQLEKGESILLCSDGLHEQLAPEAIQAI"
     gene            6132..6953
                     /locus_tag="PWY43_001900"
     CDS             6132..6953
                     /locus_tag="PWY43_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDL2136759.1"
                     /translation="MKNDFHYAAELIRNADGILITAGAGMSVDSGLPDFRSVGGLWNS
                     YPPLKKFNLQFMQIATPLAYQERPELAKWFYAHRLNQYRQAEPHQGYAILKRWAESKQ
                     HGYFVFTSNVDGHFRKAGFSEDRIYECHGTLDHLQCLANCKNKSWWAYDYQPVTDDEH
                     CLVLSEPPTCPDCGGLARQNVLMFNDWSYAEGYQLTKETLLDEWLRKAQRPVVIEIGA
                     GKVVPTVRNFSERTAKQKKGGLIRINPIDAGVPKMHFLSLEMGGLEALQGIEECL"
     gene            7029..>7794
                     /locus_tag="PWY43_001901"
     CDS             7029..>7794
                     /locus_tag="PWY43_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDL2136760.1"
                     /translation="MSQNNTEKEIAEIRKIEDVVRTKLSIPDYQRPYKWSVKHITQLL
                     NDLYFHFENGKKVYRVGSVVIHTYTGKDENSESKTKFDIVDGQQRLISLSLILFLLSN
                     DNTFPLLSEKLSHSISADNLIKNKVAVEQFIRDRVQDKQEFATYILETCEFVYIELGD
                     IDEAFQFFDAQNARGKSLAPYDLLKAYHLRELKADKETIYQCVENWEQAVEAEIADLK
                     EIISETLFRLRRWQKYQTAEKFTNKELDTFKGASSDK"
BASE COUNT         2300 a         1624 c         1646 g         2224 t
ORIGIN      
        1 ctaaattgtt caatgtgcca ttatttactg gttttatgta tgcagcagtt ggttcttaca
       61 ttatccaagc gtggcggttg tttgatttga aaattaaaag ccacctgccc tattggttag
      121 ccactttaac ggcactgatg atttacctga atttctttac gcatcattac attggggatt
      181 accgttggta tattgccgct tttgcattag ggctgtatgc acgtactttt gtatttttca
      241 ccccttatga cattacacga aaaatgccac tgctgctttc tttcttcctg ataggttttt
      301 tcatttggtt ggcggaaaat atcggtacgt ttgccggaat ttggcgctac ccgaatcagt
      361 ttggggcttg gtctatggtg catatgagca agtggagtgc ttgggcgtta ttggtgatga
      421 tgacttttac catcattgtg catttaaagc atatcaagca aacagtcaga gtctcgagcg
      481 attaacaagc tgtgggtttt ggcgaaaatt ttactcatca gttattttca gtggaattag
      541 gggcaaataa taatttgccc ctatatcaaa ttatggattt tccccttcca catcaataat
      601 catcacttcc gactgtgagc ctaaccgcat cggcacgccc cagaatccgt agccggaggt
      661 gacaaaataa tgcccgaagc cgattttttc atagccgtaa tgtaatcgat agatcagttt
      721 ggtaataata cttgcaggga acacttgccc tttgtgagcg tgtcctgaaa cttgaatatc
      781 aatcggcaat ttagcatgtt gctcaatttc agtcgggcgg tggtcaagca aaattaccgg
      841 tagtttagtg ttcacgtttt gtaaaagctg ttcggcagaa ggacgatttt tgactaaatc
      901 atcattacgc ccaataaggg taaattttcc gttaatttcg gccgtttgat cccataaaac
      961 agtaataccg gcgtcggtaa tttcttttgc aatcgtttct tgagccccaa aaaagtcgtg
     1021 attgccaagg gtagcataca cgccaagagg agcttttaat ttggctaaat gaggctgcat
     1081 tttatcggca acataaactt cggtattgtc gtccataata tcgcccggca ataaaatcag
     1141 atcgactttt tgctggttga aaatctctgc cagttggtca agttgcttgg ttccgaacag
     1201 ttttcctaaa tgcagatcgc ttgccatacc aatgcgaagc ggtttctcta atggtttatc
     1261 caatttcacc gaataatgta ctactgttgg ggtataagcg ttgtaatagc caagcccaag
     1321 taagccaaag aatgcaaatg ggtaggcgat tttcagccag cgattgacgg tagtattgcc
     1381 gttgaatatt ttgtaaatac aagcggtgaa aaaactgata aaaaatgcaa aaagtaacaa
     1441 taccaatatg aatgccatta ttctaaatag cgcaaaagta cgagtaagat gcaaaatcac
     1501 taatccgttt gcgactaaga aagtgagaat accaactgtt ttacggtttt tacttgataa
     1561 attaaataac cagctgacag tctttgcaaa gttgaataaa aagagttgta acaccgcaat
     1621 cacaagggcg gtggtaatgt agtaacgata ttccatattt ttccttcaat aaattggcta
     1681 aacccagccg atttgtctta ataaaaataa gccgatgctg gcactaaaca ttgagccgag
     1741 agtcgtaatg ccgatcagat ttgcggctgc ctcgccgtct ccgccatagt tgcgtgtcat
     1801 tgcgtaggtg gctgaagcaa cgggagcaga agcagccaca aagacaatgc ctagttccat
     1861 cggcggaagt tgaaacacaa atttcccgag tagtagcaag aaaattggag caaaaatcag
     1921 ccgaatcgag gcagaaaaca gcacaatttt attgatggtg ctttccactc cttgattaaa
     1981 ttgtttcaac tgtttgaaat taatgcttgc tccggtgcag ataagtgcta atggtagtgt
     2041 caggctcgcc attaatttac cagcagactc aagcggggtt ggaatttggt ggcttaattc
     2101 aaaatagtta aaagttaagc ctgcaatgac ggctagaatc agcggatttc tcaatatcga
     2161 caatattaat ctgccaaaat tcactttttt ctcgcttaac gatgaagtga gggtaatcac
     2221 tgcaagcaca ttaaaggtaa tcaccaagca cgaaatataa actgaagcgg cagcgagagc
     2281 tttatcgcca taagcattga gaattaaggc aatgcctaaa atcgccgcat tggtacggaa
     2341 agtgccttga gtgaaaatac aacgataacg gcggttttca atatgtcgag ccgcccacca
     2401 ttccgcccat aaatagatca aaatcgaacc gccgactccg gtcgagatca ggttgatttg
     2461 gctggagaaa tctaacgggc tttttagcat attcaagaaa atcatcggtg gcagggtaaa
     2521 attaaatacc acctttgatg ccatattgca aaaggtatcg tcaataaatt ttcgtctgcg
     2581 tggaaaaata ccgaacagca ttaacaaaat ggttggtaag gtaacgccaa tgctaaattg
     2641 gatagagtcg aaaagcataa tgtttgcctt aaatgatggc acgagtgtct tttgcttgta
     2701 ccgtagatta tcaataaagt accagcgtgg acgctggtac tatcatcaat ggaaatgaaa
     2761 ttacaatcct gcctttaagc ttgcttcgac aaatttatcc aaatcaccgt ccagcacttg
     2821 ttgggtggtg cgatgttcca caccggtacg gtggtcttta atccgtgaat cgtctagcac
     2881 ataagagcgg atctggctgc cccagccgat gtcggatttg ttgtcttcca tcgcctgttt
     2941 ttccgcattt ttcttgctca tttccagctc atatagcttc gctttaagct gtttcatcgc
     3001 ctgatcttta ttttgatgtt gtgaacgacc gttctggcac tgcaccacaa tgccgctcgg
     3061 tatatgggta attcgcaccg ccgattcggt tttattgacg tgctgaccac ccgcccctga
     3121 ggcacgatac acatcaatcc gcaaatccgc agggttgatt tcaatatcaa tatcgtcatc
     3181 gacttccgga taaacgaaag ccgccgagaa cgaggtatga cgaccgttat tggcatcaaa
     3241 cgggctttta cgcaccaagc ggtgaatacc cgtttcggtt ctgaaccagc cgtaagcata
     3301 gtcgccggaa attttcacgg tggcggattt aatccctgcc acatcaccgt ctgaggcttc
     3361 cattagttcg gctttaaacc ctttgctctc tgcccaacgc aaatacatac gcagtaacat
     3421 ttctgtccag tcctgagctt ccgttccacc cgaaccggct tgcaaatcca aatagcaatc
     3481 ggcactgtcg tgtgggccgc tgaacatccg cttaaattct aaatcggcaa gttttttctc
     3541 taattcgtct gcttccactt gggcttcatt gaaggtatcc tgatcttctg cttccaccgc
     3601 cagctcaatc aacccttcaa catcttcaat cccttgctct aaggctttaa tcgtattgac
     3661 gatctgctcc aacattgccc gctctttacc aagcgcttgt gctttttccg gcttgttcca
     3721 tacatcgggt tgttccagct cggcattgac ctcttctaag cgttcgactt tatgctcaaa
     3781 gtcaaagata ccccctgatc accgcggtgc gctcgcttaa atcagttagt tggtttttaa
     3841 ttgggtttaa ttcaaacatt gtcgttcctg tttttgctta ttttaaaaag atgaattata
     3901 gctgaagtta agcaagcggt cgaattttgc aagaaatgga gcaatgatcg tttctagtaa
     3961 tgagatttgg ttcataaaag ttgcaactgt ttctgctatt tggctttgat agctaaaaat
     4021 ctttaaaaaa acaagcggtc gttttaccac attttttgca aaacgaccgt agcttcttgc
     4081 tataatcgct gaaaaaatgc tgcaaggaga ttttatggca aagcgcccaa gtgatattga
     4141 tgcaacccta ttgcaactgg aaatcctacg ccgtatcccc aaattacccg ctaaaattga
     4201 tgctaaaagt ttgcaccaac agctattaga tgccggcttt gaacgggata ttcgcactgt
     4261 tcagcgttcg ctcaaaatgt tatgcgagca ttttgatatt gaatgcgatg agcggagcaa
     4321 accctttggt tatagctgga aaatccgctc agaggggctg gcattgccga ttctcaacga
     4381 acaacaatca ttactgctaa aacttgccga gcaacagctg aaatatctgt tacccgcaaa
     4441 tttaatggca tcaatgaaac ccttttttga gcaggcagac agaatggttt ccggggcgaa
     4501 agacaagccc gaataccaat ggctaaataa agtttgctcc acgccaacca gcctaccgct
     4561 gatccctgca aaaattaaag aagagatttt taattccgta agccaagcct tatttcaaaa
     4621 taagttgtta aagatcgaat atcagaacca gcacggcaaa aaacaccaag cgacaattat
     4681 gccgttggcg atttcacaac aaggtgcgag catttatttg gtctgccgtt acgaagggtt
     4741 tgacgacaac cgcctgttgg ctcttcaccg cattcgtaaa gcggagattt ctacttttag
     4801 ttttgaacgc ccgaaagatt ttaatttgca agcctaccaa gatgccggac acctcggttt
     4861 tggcaatggc gaaaaagcta gccttacctt ttcgattagc agagacgccg gtttccattt
     4921 aaccgaaaca ccgcttgcca aagatcaaaa aattctcgag gagagtgaaa ctcactaccg
     4981 tattcaagcc atagtcgctg ataatgatat gctaacctgg tggctacgca aatttggcga
     5041 agatatttgg gatatcgaga aagaagcgct aaaataagct aaacaagcgg tcggttttgt
     5101 gggaattttt gctaaatagt atcgaccgct cctccctaaa cagaccttta tcataaatac
     5161 aactctttca atcagatatt cttactgtct attgatgaac gattcgaatt tttctagtgg
     5221 ttgaaattta aactaatttt cttgcttttt ggcatttttc agacttttaa ttctctccat
     5281 tagcctgttt ttttacgctc tttttcataa acaggaataa ccaatgcctt ttcaacttac
     5341 tttctgccaa caagccggca atcagcacca gaaaaatcaa gatgcactgt ttaacggcaa
     5401 agcggtgtac caatggcaac ttaaaaatgc cgagagttat ctgctggata acgacaaagt
     5461 aatttttggg gttgcggacg gcgtttccag ccaccctcga aatcatcttg cctcacatta
     5521 tttaatggcg ttactcagcc aatgccaatc ccttgatagc caatggttgc gacaggctca
     5581 cggcgatttt tgcgaaaaat tagcgaaaga ttattttggt gcttcttgca cttttgttgc
     5641 ttgtgaatta caccataacg gcagagggcg tatggttaat gtgggcgaca gtcgagccta
     5701 taaaattacg gcaaacgggg agtggcaaca actgagtttt gatcacacca tactggctga
     5761 aatgaaacag aaaggcttgg ttaaggaagg gagcaactat gcctcaatgt ataacggctt
     5821 aaccgattgt attgttgccg attttgacgc cgatgatttc cgcattcata tagccgaatt
     5881 tcaactggaa aaaggcgaga gcattttgct ctgttctgac ggtttgcacg aacaactcgc
     5941 cccagaagct attcaggcga tttaggacgg atacgaaagc ccgtctcatc gtctgcaaat
     6001 ttgcagaaaa atggtaaaaa tgcaccgctt gtatgacgat ttttctgcgg tgatttgtga
     6061 aattgtgtaa ttaaaacgac attatttgtc gcattggtgc ttaaaatccg cataaaacag
     6121 aggagaaaaa aatgaaaaac gatttccatt atgccgctga attaatccgc aatgcagacg
     6181 gcattttaat taccgcaggg gctggaatgt cggtggacag tggcttgccc gatttccgta
     6241 gcgtgggagg gttatggaat tcctacccac cgctcaaaaa atttaatttg caatttatgc
     6301 agattgccac accgcttgcc tatcaggaac gccctgagtt ggcaaaatgg ttctatgccc
     6361 atcgcctaaa tcaataccgc caagccgaac cgcaccaagg ctatgccatt ctcaaacgct
     6421 gggcggaaag caaacaacac ggctatttcg tcttcaccag caatgtggac gggcatttcc
     6481 gcaaagccgg tttttcggag gatcggattt acgaatgcca cggcacgcta gaccatttgc
     6541 aatgtttggc aaattgcaaa aataaatcgt ggtgggcgta cgactatcaa cctgtaactg
     6601 acgatgaaca ttgccttgta ttgagcgaac caccaacttg ccctgactgc ggaggattag
     6661 cccgccaaaa tgtactgatg tttaatgatt ggagttatgc cgaaggctac caacttacta
     6721 aagagacact gttagatgaa tggctacgca aagcccaacg ccctgtggtg attgaaattg
     6781 gtgcaggcaa agtcgtacca actgtgcgta atttttccga acgcaccgcc aagcagaaaa
     6841 aaggtggttt aatccgcatc aacccaatag atgccggcgt acccaaaatg cactttttaa
     6901 gtttggaaat ggggggatta gaggcgttgc aggggattga ggaatgctta taagcggtct
     6961 aaattcacca agactttgca aattttgcta caataaaaac attgaaatat tgtaataaag
     7021 gatctgaaat gagccaaaat aatacagaaa aagaaattgc ggaaattaga aaaattgaag
     7081 atgttgtaag aacgaaatta tctattcctg attatcagcg cccttataag tggtcggtaa
     7141 aacatattac ccaattatta aacgatctct attttcattt tgaaaatggt aagaaagttt
     7201 atcgagtagg ttcggttgtt atccatacat atacaggtaa agatgagaat agtgagtcta
     7261 aaacaaaatt cgatatagtt gatggacaac aacgtttaat tagcctttct ttaattttat
     7321 ttttattgag caatgataac acttttccac ttttatctga aaagctctca catagtatta
     7381 gtgcggataa tcttataaaa aataaggttg cagtagaaca atttattcga gatagagtac
     7441 aagataaaca ggaatttgct acctatattt tagaaacttg cgagtttgtt tatattgaat
     7501 taggggatat tgacgaggct ttccaatttt ttgatgctca aaatgcaaga ggaaaatcgc
     7561 ttgcacctta cgatctatta aaagcgtatc atttaagaga acttaaagca gataaagaga
     7621 cgatttatca atgtgtagag aattgggagc aagcagtaga agctgagatt gctgatttaa
     7681 aagaaatcat cagtgagacg ttatttagat tacgccgttg gcaaaaatat caaacagcag
     7741 aaaaattcac gaataaagaa ctcgatacat tcaaaggtgc tagctcggat aaaa
//
LOCUS       DANKBV010000110         7785 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.110, whole genome shotgun sequence.
ACCESSION   DANKBV010000110 DANKBV010000000
VERSION     DANKBV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.110"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            52..1446
                     /gene="murF"
                     /locus_tag="PWY43_001902"
     CDS             52..1446
                     /gene="murF"
                     /locus_tag="PWY43_001902"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL2136761.1"
                     /translation="MIKLTTQQIAEILNAKLIGNGETVVETTSTDTRQAVENGLFFAL
                     KGENFDAHHYLQNAVAQGCVAVVVERECEIDVPQLVVADTRLALGELAKWLKAKLNPK
                     TVAMTGSSGKTTVKEMTAKILQKMTACEQEVLYTFGNLNNDLGVPMTLLRLTEQHKYA
                     VIELGANHIGEIAYTTAIAQPDACLVNNVAAAHLEGFGSLAGVAQAKGEIYRGLKENG
                     KAIVNLAFYYPQWQKEIDSREMQSFSYIGSDSDSYADYWAEDVELHLSGSRFTLHTPQ
                     GEIAIDLPYLGKHNVSNALAATSLAMSVGATLEAVKAGLEQRSMVKGRLYPVQINDHC
                     LFIDDSYNANVDSMKSAISVLENYPAYRIFAVGDMAELGEESSKCHQEVADFAKNAAL
                     DLIVSFGKESAVISQQAEHHFTDKQQMVDFLLPIILQKIEQKQPLVLLAKGSRSQKME
                     TVISELCKPFNLNF"
     gene            1467..2549
                     /gene="mraY"
                     /locus_tag="PWY43_001903"
     CDS             1467..2549
                     /gene="mraY"
                     /locus_tag="PWY43_001903"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL2136762.1"
                     /translation="MLVWLAELLVQYNTAFNVVSYITFRAIMALLTAMGIGLWIGPKV
                     IRRLQILKFGQEVRNDGPESHFKKRGTPTMGGVMILAAIGVSTLLWADLRNSYVWFTL
                     FVLFGYGAVGFVDDYRKISRKNTDGLIARWKYFWLSAIALVAVFGMYAVGKDTAATQL
                     VVPFFKDVMPQLGLFYIVLSYFVIVGTSNAVNLTDGLDGLAIVPTIMVASAFALIAWA
                     TGNFNFAQYLHIPFIPNAGELVILCTAIVGAGLGFLWYNTYPAQVFMGDVGSLSLGGA
                     LGVIAVLVRQELLLVVMGGVFVVEALSVILQVGSYKLRQKRIFRMAPIHHHFELKGWP
                     EPRVIVRFWIITLMLVLVGLVTLKLR"
     gene            2613..3914
                     /gene="murD"
                     /locus_tag="PWY43_001904"
     CDS             2613..3914
                     /gene="murD"
                     /locus_tag="PWY43_001904"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL2136763.1"
                     /translation="MQKQYENKTVTIIGLGTTGLSCVDFFKKKNANIQVIDTRANPAG
                     ADKLDVTIPLHTGGLNSEWLLKSDLIVISPGLAVATPEIQQAIQAGVEIVGDVELFCR
                     EAKAPIIAITGSNGKSTVTTLTTEMAKQAGIKVGMGGNIGVPVLSLLEGDYDLFVLEL
                     SSFQLETTYSLKAKAATVLNISEDHMDRYDSIADYRQAKLRIYDNAENIIVNGEDAQT
                     YPIQAVKKVIRFAEQNAEYSLRNQMLYSGDDAIIQTKQMLISGRHNEMNALAAMALAE
                     AANVPREGIVKALQIYGGLPHRFQAVATNDGVRWVNDSKATNVGSTVAALNGLNIAGT
                     LYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAALAELSEQSVLVETMQQAIEAI
                     RPQLKKGDMVLLSPACASLDQFKNFEQRGEVFMALAQGQHA"
     gene            3914..5092
                     /gene="ftsW"
                     /locus_tag="PWY43_001905"
     CDS             3914..5092
                     /gene="ftsW"
                     /locus_tag="PWY43_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HDL2136764.1"
                     /translation="MLERIKSEWDKWVRLTPSNSLYDRTLIWLFLGLLIIGFVMVTSA
                     SLPVSTRLNNDPFYFAIRDGLYIIASIIFCYVFVQIPIEKWEKHNLALFFISIGFLIA
                     VLIFGRSINGAVRWIPLGILNFQPAELAKLAVICYFASFYVRKYDEIRKEKASFWRPA
                     VILFLFGFLLILQPDLGSTFVLFVLTFSMLFIVGAKIMQFMFLGVVGTVLFAVLILTS
                     EYRLKRVTSFMDPFADAYGDGFQLSNSQMAFGQGEFWGQGLGNSVQKLEYLPEAHTDF
                     VMAVIGEEFGFFGIACIVLLLILLTVRALKISKESLVLEERFKGYMAFGIAIWVFLQG
                     FVNLGVASGLLPTKGLTFPLVSYGGSSLVIMSIAIAVLLRIDHENRAERIGHAKIKTI
                     "
     gene            5211..6266
                     /gene="murG"
                     /locus_tag="PWY43_001906"
     CDS             5211..6266
                     /gene="murG"
                     /locus_tag="PWY43_001906"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL2136765.1"
                     /translation="MAKKLLVMAGGTGGHVFPAIAVARELQKQGWQIRWLGTKDRMEA
                     TLVPKHGIEIDFIEISGLRGKGVAALLKAPFAIFKAVMQARKIIKNYQPDAVLGMGGY
                     VSGPGGIAAKLCGVPVILHEQNAVAGLTNVWLSKIARKVLQAFPTAFKEAEVVGNPVR
                     EDLSALPLPNERFAERGYPINILVMGGSQGARVINQTVPEVAKVLGNNVFISHQVGKG
                     NLAGIEEVYQQTGNGIASEFIDDMKAAYEWADLVICRSGALTVCEIAAVGLPAVFVPF
                     QHKDRQQFLNANYLAADGAAVIIEQSDFTPKALQQALEPLIANRQLLLDMAMRARAKS
                     TPMAAKRVAEVIIQEAN"
     gene            6342..7769
                     /gene="murC"
                     /locus_tag="PWY43_001907"
     CDS             6342..7769
                     /gene="murC"
                     /locus_tag="PWY43_001907"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL2136766.1"
                     /translation="MKNFQDKVKKLVPEMRRVSQIHFIGIGGAGMSGIAEVLLNEGYQ
                     ISGSDIADRAVTQRLSAAGAKVFIGHQAENIAGASVVVVSTAIDETNPEIIAAREARI
                     PVIRRAEMLAEIMRFRHGIAIAGTHGKTTTTAMISMIYTEAKLDPTFVNGGLVKSAGK
                     NAHLGASRYLIAEADESDASFLHLQPMVSVVTNIEPDHMDTYGGDFEKMKETYVRFLR
                     NLPFYGLAVMCADDETVMEIAPRVGRQVITYGFSENADYRIEDYQQTGFQEHYTVVCP
                     SGERIDVLLNVPGMHNALNATAALAVAKEEGIGNEAILAALADFQGAGRRFDQLGSFI
                     RPNGKIMLVDDYGHHPTEVDVTIQAARSGWEGKRVVMIFQPHRYSRTRDLFDEFVQVL
                     SKVDALILLDVYAAGEAPIVGADSKALARSIRNLGKVDPILVSDTDQLGEVLDQIIQD
                     GDLILAQGAGSVSRISRGLAESWKN"
BASE COUNT         2170 a         1475 c         1912 g         2228 t
ORIGIN      
        1 tgacaaatac aaaatgctcg taattaattt tataaagaaa aagataaatc aatgataaaa
       61 ctaaccaccc aacaaatcgc tgaaatccta aatgccaaat tgatcggcaa tggcgaaact
      121 gtggtagaaa ccaccagcac cgacacccga caagcggttg aaaacggctt attttttgca
      181 ttaaagggcg agaactttga tgctcatcac tatcttcaaa atgcagtcgc acaaggttgt
      241 gtagcggttg tggttgagcg tgagtgtgaa attgatgtac cacaacttgt ggtggcagat
      301 acccgtttgg cattggggga attggcgaaa tggttaaaag ctaagctgaa cccgaaaacg
      361 gtagcgatga caggttcttc cggcaaaaca accgtcaaag agatgacggc aaaaattctg
      421 caaaaaatga ccgcttgcga acaggaagtg ctttatactt ttggtaactt aaacaatgac
      481 ttaggtgtac cgatgacctt gcttcgctta accgaacagc ataaatatgc ggtcattgag
      541 ctgggggcaa atcatatcgg tgaaatcgct tataccaccg ctatcgctca gcctgatgcc
      601 tgcttggtga ataatgtggc tgcggcgcat ttagaggggt ttggttcatt agcaggcgtg
      661 gcacaagcca agggcgaaat ttatcgtggc ttgaaagaaa atggcaaagc gattgtcaat
      721 ttagcttttt attatccgca atggcaaaaa gagattgact cacgggaaat gcaatctttc
      781 tcttatatcg ggtcagattc ggattcttac gcagattact gggcggaaga tgtagaactg
      841 catttaagcg gttcacgctt tacgctacat accccacagg gcgaaattgc tattgatctg
      901 ccttatttgg gcaaacataa cgtgagcaat gccttagcgg caacttcgct ggcgatgtct
      961 gttggggcaa cgctagaagc agttaaggca gggcttgagc agcgttcaat ggtaaaaggg
     1021 cgtttgtatc cggtgcaaat taacgaccac tgtttgttta ttgatgactc ttacaacgcc
     1081 aatgtggatt caatgaaatc ggcgatttct gttttggaaa attatccggc ttatcgtatt
     1141 tttgcagtgg gtgatatggc ggaattgggt gaagaaagta gcaaatgcca ccaagaagta
     1201 gcggattttg ccaaaaatgc agcattagat ttaatcgtga gctttggaaa agaaagtgcg
     1261 gtaatcagcc aacaggcaga acatcatttt accgataaac agcaaatggt tgatttccta
     1321 ctgccgatta ttttgcaaaa aatcgaacaa aaacaaccgc ttgtattatt agcaaaaggc
     1381 tctcgtagcc agaaaatgga aaccgtgatt tccgaattgt gtaaaccgtt taacctaaat
     1441 ttctaattat ttttaaggac taagcaatgt tagtttggct tgcagaacta ttggttcaat
     1501 ataacacagc gtttaacgtt gtctcttata ttactttccg tgccattatg gcattattaa
     1561 ctgcaatggg aatcgggctt tggatcgggc cgaaagtaat tcgccgtttg cagatcttaa
     1621 aatttggtca ggaagtgcgt aatgacggac cggaaagcca tttcaaaaaa cgtggtacgc
     1681 caacaatggg cggagtgatg attctcgcag cgattggggt gagtacattg ctttgggcag
     1741 atctgcgtaa ttcctatgtt tggttcacgc tgtttgtgct atttggctac ggtgcagtgg
     1801 gctttgttga tgattatcgt aagatctctc gtaaaaatac cgatggtttg attgcccgtt
     1861 ggaaatattt ttggttatct gctatcgcat tagtggcggt gtttggtatg tatgcggtgg
     1921 gtaaagatac cgctgcaaca caattagtcg tgccgttttt taaagatgtg atgccgcaac
     1981 tcggtttatt ttatattgtg ttatcctatt tcgtgattgt cggcaccagt aatgcggtaa
     2041 acctaacgga cggcttagac gggcttgcga ttgtgccaac tattatggtc gcatctgcct
     2101 ttgcgttaat tgcgtgggca accggtaact ttaattttgc acaatattta catattcctt
     2161 ttattccaaa tgccggtgaa ttggttattt tatgtactgc gattgtggga gcaggtttag
     2221 ggttcttatg gtataacacc taccctgcac aggtgtttat gggcgatgtt ggctcacttt
     2281 cgctcggcgg tgcattaggc gtgattgcag tattagttcg ccaagaatta ttattggtgg
     2341 taatgggcgg tgtatttgtg gttgaagccc tctcggtgat tttacaagta ggttcataca
     2401 aattacgcca aaaacgtatt ttccgaatgg ctccaattca ccaccatttt gaactcaaag
     2461 gctggcctga accacgggtt atcgtgcgtt tctggattat cacgctaatg ctggttttag
     2521 tcggcttagt aacattgaaa ttacgttaat tgattgaata aggtgcattg agataatgta
     2581 ccttatgtta tttttaaatt acagaataaa ttatgcaaaa acaatacgaa aacaaaaccg
     2641 ttactattat cggcttaggt acaacagggt tatcttgtgt ggatttcttt aagaaaaaaa
     2701 atgccaatat ccaagtgatt gacacccgag caaacccagc cggagcggat aaattggacg
     2761 taactattcc gttgcatacc ggtgggttaa acagtgaatg gttgctgaaa tctgatctta
     2821 ttgtaattag cccaggttta gccgttgcca cacctgaaat tcagcaagcg attcaagctg
     2881 gtgttgaaat agtcggtgat gttgaactgt tttgtcggga agcgaaagct ccgattattg
     2941 cgatcaccgg ttcaaacggc aaaagtaccg taacgacttt aactaccgaa atggcgaagc
     3001 aagcggggat taaagtggga atgggtggca acatcggtgt gccggtatta agtttgctgg
     3061 agggcgatta tgaccttttt gtgctggagc tatccagctt ccagcttgag acaacttatt
     3121 cactgaaagc aaaagctgcc actgttttaa atattagcga agatcatatg gatcgctacg
     3181 attcgattgc cgactatcgc caagcgaaat tgcggattta cgataatgcg gaaaatatca
     3241 tcgtaaacgg tgaagatgcc caaacttacc cgatacaagc ggtcaaaaaa gtgattcgtt
     3301 ttgcagaaca aaatgcggaa tatagcctac gcaatcagat gctttattca ggcgatgatg
     3361 caattatcca aactaaacag atgctgatta gcggccgtca taatgagatg aatgcgttag
     3421 cggcgatggc attagcagaa gcggcgaatg ttcctcgtga aggcattgtg aaagctttgc
     3481 aaatttatgg tggtttgcca caccgtttcc aagcggtggc aaccaatgat ggcgtgcgtt
     3541 gggtcaatga ttccaaagcg accaatgtgg gtagcacggt agcggcatta aacggcttga
     3601 atattgccgg tacactttat ttacttttag gtggtgacgg caaggctgcg gatttttccg
     3661 aactcaaacc attaattaat aagccaaata ttgtctgcta ttgtttcggg caagatggag
     3721 cggcattggc agaattaagc gaacaaagtg tgttggttga aacgatgcag caagcaattg
     3781 aagcaatccg cccacaactg aaaaaaggcg atatggtact gctttctccg gcttgtgcca
     3841 gtttagatca gtttaaaaac tttgagcaac gtggcgaggt atttatggca ttagcacaag
     3901 gacagcacgc ataatgttag aaagaattaa gtcggaatgg gataaatggg tcagattaac
     3961 cccttctaat tcactttatg acagaactct aatttggcta tttttaggcc tgctaattat
     4021 cggttttgtg atggtaacat ctgcttcact cccagttagc acaaggttga ataacgatcc
     4081 attttatttt gcgattcgag acgggctgta tattattgct tcaattatct tttgttatgt
     4141 ttttgtacaa atcccaattg aaaaatggga aaagcataac ttggcactgt tttttatttc
     4201 aatcggtttt ttaatcgccg tactcatttt cggccgctca attaatggtg cagtgcgttg
     4261 gattccgttg ggtattctca actttcagcc ggcagaatta gccaaattag cggtcatttg
     4321 ctattttgcc agtttctatg tacgtaaata tgatgaaatt cgtaaagaaa aagccagttt
     4381 ctggcgaccg gcagtgattt tattcttatt cggcttcttg ctgattttgc agccggattt
     4441 aggtagtacc tttgtattat ttgttctcac tttctcaatg cttttcattg tgggggcaaa
     4501 aattatgcaa tttatgtttt taggcgtggt gggaacggtg ttatttgcgg tgttgatttt
     4561 aacctcggaa taccgtttaa aacgtgttac ctcctttatg gatccgtttg ctgatgctta
     4621 tggcgacggt ttccagctct cgaactcaca aatggcattt ggacaaggtg aattttgggg
     4681 acaaggttta ggtaattcgg tacaaaagct agaatatttg ccagaggcac atactgactt
     4741 tgtaatggcg gttattggtg aagaatttgg tttctttggt attgcttgca tcgtactttt
     4801 acttattcta ttaacggtga gagccttaaa aatcagtaaa gaatctttgg tgctggaaga
     4861 gcgttttaaa ggctatatgg catttgggat tgcgatttgg gttttcctac aaggcttcgt
     4921 aaacttaggg gtggcatcag gtttgctgcc aacgaagggc ttaaccttcc cattagtgag
     4981 ttatggtggt tcgagtttgg taattatgtc gattgcgatt gcagttttgc tacgaattga
     5041 ccacgaaaac cgtgccgagc gaatcgggca cgcaaaaatt aaaaccatct agccgagcgg
     5101 tctgcaacgt tacaatttgc aaaattttac aaaaatgaga ccgcttgtat tagctggaaa
     5161 gcataaaaat tcagtaaaat agcacgtttt agaataaaaa agagttgaaa atggcgaaga
     5221 aattattagt aatggcaggt ggaacagggg ggcacgtttt ccctgcaatt gccgtagctc
     5281 gagagctaca aaaacaaggc tggcaaatcc gctggctggg gacgaaagac cgaatggaag
     5341 ccactcttgt gccgaaacac ggtattgaga ttgattttat tgaaatctca ggcttgcggg
     5401 gtaaaggcgt tgcagcattg ttgaaagcac cgtttgctat ttttaaagcg gtgatgcaag
     5461 cccgcaaaat cattaaaaat tatcagcctg atgcggtgtt gggaatgggc ggttatgtgt
     5521 ccggtccggg cggtattgcg gctaaattat gtggcgtgcc ggtgatttta cacgagcaaa
     5581 acgcagttgc cggcctaacc aatgtgtggc tatccaaaat tgcccgcaag gtgttacagg
     5641 ctttccctac tgcttttaaa gaggccgaag tagtaggtaa tcctgtacgt gaagatttat
     5701 ctgctcttcc gctgccgaat gaacgtttcg ccgaacgagg ctacccgatt aacattttag
     5761 taatgggtgg cagccaaggg gcaagagtga tcaaccaaac cgtgccggaa gtggcaaaag
     5821 tgttgggtaa taatgtgttt atcagccacc aagtcggtaa aggtaattta gccggtatcg
     5881 aagaagtcta tcaacaaacc ggcaatggga ttgcttccga gtttattgat gatatgaaag
     5941 ccgcgtatga gtgggcagat ttggtgattt gtcgttccgg tgcgttaacg gtgtgtgaaa
     6001 ttgccgccgt aggcctgccc gctgtgtttg tgccgttcca acataaagac aggcaacaat
     6061 ttttaaacgc taactatttg gcggcagatg gagcagcagt gattatcgaa cagagcgatt
     6121 tcactccaaa ggcattacag caagccttag aacccttaat tgccaatcgc caactcttat
     6181 tggatatggc aatgcgagcc agagcaaaat ccaccccaat ggcagcaaaa cgtgtagcgg
     6241 aagtgattat tcaagaagcg aattaactta ctacaagcgg tcgtttttgg taaaaatttt
     6301 gcaaaatgta accgcttgcg aatagattaa aaggacaata aatgaaaaat ttccaagata
     6361 aagtcaaaaa attagtgcct gaaatgcgtc gtgtcagcca aattcatttt atcgggattg
     6421 gcggagccgg tatgagcggc attgcggaag tgttattaaa cgagggatac caaatttcag
     6481 gttcggatat tgcagacaga gcggttacac aacgtctatc cgcagccgga gcgaaagtct
     6541 ttatcggaca ccaagcagaa aatattgctg gggcgagtgt ggtggtggtt tctactgcga
     6601 ttgatgaaac caaccctgaa attattgccg ctcgagaagc tcgaattccg gtgattcgcc
     6661 gagcagaaat gcttgccgaa attatgcgtt ttcgccacgg cattgcgatt gcaggcacac
     6721 acggtaaaac cactacaacc gcaatgattt caatgatcta caccgaagca aaattagatc
     6781 cgactttcgt gaatggcggt ttggtaaaat ctgccggcaa aaatgctcat ttgggggcga
     6841 gccgttattt aattgccgaa gccgatgaga gtgatgcttc tttcttgcac ttgcagccaa
     6901 tggtgtcggt agtaaccaac attgagcctg accatatgga cacctatggt ggcgattttg
     6961 agaaaatgaa agagacttac gtacgatttt tgcgtaactt acctttctac ggcttagcgg
     7021 taatgtgtgc cgatgatgaa acggtaatgg aaattgctcc tcgtgtcggc agacaagtga
     7081 ttacctatgg ctttagcgaa aatgcggatt accgaattga agattaccag caaaccggct
     7141 tccaagagca ttacaccgta gtttgcccaa gcggtgaacg aattgatgtg ctgttgaatg
     7201 tgccgggtat gcacaatgcc ttaaatgcaa ccgctgcgtt agcggtggca aaagaagaag
     7261 ggattggcaa tgaggcgatt ttagctgctt tagctgattt ccaaggggca ggacgccgct
     7321 ttgaccaatt aggttcattt attcgcccga acggcaaaat tatgttagtt gatgactacg
     7381 gccaccaccc aactgaagtt gatgtaacca ttcaagccgc tcgttcaggc tgggaaggta
     7441 aacgtgttgt gatgattttc caaccgcacc gctattctcg tacccgtgat ttattcgatg
     7501 aatttgtaca agtactgtct aaagtcgatg ccttgatttt attggatgtg tatgctgccg
     7561 gcgaagctcc gattgtgggg gcagacagta aagccttagc ccgttcaatc cgtaatttag
     7621 gtaaggtcga tccgattttg gtttcagata ccgatcaact tggcgaggta ttagatcaaa
     7681 ttattcaaga cggtgattta attttggcac aaggtgcggg cagtgtaagc cgcatttctc
     7741 gtggcttggc tgagagctgg aaaaattaaa tactctctcc ccttg
//
LOCUS       DANKBV010000111         7531 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.111, whole genome shotgun sequence.
ACCESSION   DANKBV010000111 DANKBV010000000
VERSION     DANKBV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.111"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..640)
                     /locus_tag="PWY43_001908"
     CDS             complement(<1..640)
                     /locus_tag="PWY43_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136767.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKN"
     gene            complement(836..1696)
                     /locus_tag="PWY43_001909"
     CDS             complement(836..1696)
                     /locus_tag="PWY43_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL2136768.1"
                     /translation="MSIKKVTLASLILLGLTACSSGGSGSSSNNVAKPNTKPQTTQQQ
                     TDKAAAEKAAKDKAAKEKAEAERLAAEKAAKEKAEAERLAAEKEVDPIAIIKAKGIDD
                     TNYGLKLGNSKETIEGKVTVRTQSYLYNQPYSVVTAQAVQRNGWENGNYIDKVDGTVT
                     VKGLKTEKLPTEGTATYSGKAFNHDGAKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLI
                     VLEEGTINNTQISSTVHGNNPLNLSGKYTVEFFGKNAEEIAGEIQTKANLTGEVSNFG
                     LAGTRGDIQK"
     gene            1899..3353
                     /locus_tag="PWY43_001910"
     CDS             1899..3353
                     /locus_tag="PWY43_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDL2136769.1"
                     /translation="MSEISTLAPQLLWQWFDKACAIPHPSYHENELAEFIVNWAKSKN
                     FFVERDEVGNVLIRKPATKGMENRQTVALQAHLDMVPQANTGTVHDFTKDPIQPYIDG
                     EWVKAKGTTLGADNGIGLASCLAVLDSDDLAHPTIEVLLTMTEEAGMEGAIGLRPNWL
                     TADIMINTDTEENGEIYIGCAGGENVNVTLPIFLVPHQQDSAVTVTLKGLNGGHSGCD
                     IHTTRANAIKVFARILEQAHNEVEFQIAEIQGGSVRNAIPREAHATLVFSAKNQQKLT
                     ACLSQTAEQVKAELKIAEPNFQFLIEEAQLPEQAFDLASTARVIHFLNVMPNGVVRNS
                     DVVENVVETSLSIGVLNTEASEVKATILARSLIEGGKEEIRSKIRSLTALVGAEVEFS
                     GNYPGWEPNPNSKITPLTKAIYDEILGYESQIKVIHAGLECGLINKIYPNMDFVSIGP
                     TILNAHSPDEKVHIPAVEIYWKLLTKLLEQAPEK"
     gene            complement(3428..4717)
                     /locus_tag="PWY43_001911"
     CDS             complement(3428..4717)
                     /locus_tag="PWY43_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2136770.1"
                     /translation="MDIRQKIAHNKMTSFQWLVVGLTVLLNMLDGFDLMALAFTAKSI
                     REELQLDGAAIGSLMSAGLFGMAIGSLFLSPLADKFGRRPLLLIGTGLATLGMLLTYF
                     ATSAFEIGVWRVLTGLGVAAILTGTNVLVSEYSSQKWRGLAIAIYASGFGIGAMLGGM
                     SAVLLQGEYGWRAVFLVGSILTGAVFVLLFFVLPESVEFLISKHPVNAKARLNQIAVK
                     LGLGNGWELPEIEAKSKSKVAITRLFEPEYRRNTLLIWLAFIAISASFYFISSWTPAL
                     LEEAGMPKTQSQTVGMAISVGGTIGSLIFGFLVSRWAAKHILQAFSILAASAVVAFVF
                     AGNLGLAITLAILVGGLINGCITGLYTINPTLYAPDFRSTGVGTAIGVGRLVSIVSPT
                     LAGILLDAGWKKNELYLGAAVVLLIAAFTVHFLKQRK"
     gene            complement(4921..5256)
                     /locus_tag="PWY43_001912"
     CDS             complement(4921..5256)
                     /locus_tag="PWY43_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDL2136771.1"
                     /translation="MVSEQRQNPEEQKSEEQKINVEVLYAYPDKYFLKKLQVPQNATI
                     QTIILLSGVLEKYTEIDLRENKVGIFSRPAKLNETVEEGDRIEIYRPLIADPKEIRRK
                     RAAEQAEKK"
     gene            complement(5246..5680)
                     /locus_tag="PWY43_001913"
     CDS             complement(5246..5680)
                     /locus_tag="PWY43_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HDL2136772.1"
                     /translation="MPIVNQSALVPYSAEQMYQLVNQYEKYPEFLSGCVSTKTLSTGE
                     NELKAELHIQKLGISQTFSTHNKMTPPYKIEMALIEGPFRHLHGAWTFTPFDEQSCKI
                     ALQLNFEFSSPIVAMVFGKVFNELTLKMVNAFKQRAKEVYGV"
     gene            complement(5735..6790)
                     /gene="modC"
                     /locus_tag="PWY43_001914"
     CDS             complement(5735..6790)
                     /gene="modC"
                     /locus_tag="PWY43_001914"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDL2136773.1"
                     /translation="MLKLKLLQQLGNLTLNVNLDLPNKGITAIFGRSGAGKSSLINLI
                     AGLSTAQAGFIELNGQVLFDSQNGINLPPEKRNIGYVFQEHRLFPHYSVEKNLKYGYK
                     RSDSADFLQIVELLGIAPLLNRYPASLSGGEKQRVAIGRALLSSPNILLMDEPLSALD
                     LPRKQELLDYLDKLSARLEIPILYVSHSLEEVVRLADHLVLLENGKVTAFDTLSAVWH
                     SSAFSAWQPNHQRISLLELPVEETLPHYNMLGLSLNAQRLWVKQFPRHQIGEKLRVTI
                     ASKEVSISLQKPTQSSIRNVLKGNVWKIETHLERVDLAIQVENQQIWSTISLWSFNEL
                     AIQPGQEVYLQIKSVSL"
     gene            complement(6777..7469)
                     /gene="modB"
                     /locus_tag="PWY43_001915"
     CDS             complement(6777..7469)
                     /gene="modB"
                     /locus_tag="PWY43_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDL2136774.1"
                     /translation="MLNFTPQELNAIVLSLKVASIAVVLALPLAVWVAWLLARKQFWG
                     KNLLNGIVHLPLVLPPVVIGYLLLISMAKRGVIGQYLWEWFHFSLSFSWKGAVLASMV
                     MAFPLMVRSIRLAFEAVDPKLEQAARTLGATPLKVFFTLTLPLSFSGIIAGGVLGFAR
                     SLGEFGATITFVSNIPNQTQTIPAALFTFIETPDGEMAAARLCLVAILISLIALFCSE
                     WLAERQKRYAQT"
BASE COUNT         2195 a         1641 c         1582 g         2113 t
ORIGIN      
        1 catttttgcg gaaaatccgc ccatttacat tcacaatcgg cgaagatgag gcattattca
       61 cgttatctgt ttgggtatat tgcagataaa aatcgggctg ccacgcctca ttttcagcaa
      121 tttggacttg agtattgcgg gcaattttag caagttccgg cggtaaatgg gcttgaatag
      181 ccttaaattc ctgctctgcc gcacgaaatt gctgatcttc tgccaaaatc aacgccaaat
      241 caaaacgagg gtaaaccaaa ctcgggggct tagctaacag ttggcgatag tgaaaaactg
      301 cggtttgatg atcttttttc gcataggcaa gtgcggcttg cgcatagtgc cacaacaacg
      361 ggtcaaaatt ttcagtttgc ggataacggc ttaaaagcgg ttcaagcgtt gcccaatctt
      421 ggctgagaat cgcttggtta atttgctgtt caacattctg attgactgaa tacgcaggct
      481 gataagcggt cggtttttgt gaatttttta caacattttg ctcaaattta accgcttgcg
      541 gctgacgctc aaaggaaatc gggtcagtgt tgtgttcaat caccttttct gcccatacaa
      601 cggggctgaa cagcgaaaat aaaaggggat attttttcat aaagacaaag aaaattggta
      661 ggggcggacc tatatgttcg cctctacggg atttatcact ctctcccctt gtgggagagg
      721 gttattagaa gctgtaaatt gaaatctaaa ctattgtgtc ttatggtatc tacaagcaat
      781 atcaagacaa atgccgttta atttttcgtt aaacggcatt gaacgttaga aaaggttatt
      841 tttggatatc gccacgagtg ccggcaagtc caaaattact cacttcccca gtaagatttg
      901 cttttgtctg aatttcaccg gcaatttcct ctgcattttt accgaaaaat tcgactgtat
      961 attttccaga aaggtttaaa ggattattgc catgtactgt tgaagaaatc tgagtattat
     1021 taatcgtccc ctcttctaat acgattaagt ttccaaattc tccctcaact ctacctgaac
     1081 cggtacgctt agagaaatta acattatagg ttagatcacc accattaaaa cctttagctc
     1141 catcgtggtt aaatgctttt cctgaatatg ttgcagtacc ctctgttggt aatttctctg
     1201 ttttcaatcc tttaactgta actgttccat caactttatc aatataatta ccgttttccc
     1261 agccatttct ttgaactgct tgtgctgtta ctacagaata tggctgatta tataaataac
     1321 tctgtgttct gactgtgact ttaccctcaa tagtttcttt actattacca agttttaagc
     1381 cgtaatttgt atcatcaata ccttttgctt taataatagc tataggatct acttcttttt
     1441 ctgctgctaa acgctccgct tccgctttct ctttcgccgc tttttccgcc gctaaacgtt
     1501 ctgcttctgc tttctctttc gctgccttgt cttttgctgc tttttctgct gccgctttat
     1561 cggtttgttg ctgagtagtt tgcggtttag tattcggttt tgccacattg tttgagctac
     1621 tgcccgaacc accactacta cacgccgtta aacccaataa aatcaaactg gctaaagtta
     1681 cttttttgat agacataaaa tgttcctttt gtaagataaa caaaaataga tgtgtataaa
     1741 acacacgcat attgtacaaa aaaaaaacaa ctcgtcatca agtacttttt aagatttttt
     1801 tgttaaattt ctcctaaata ttctacaatg tcagaggtct atatcagctt gaatacaagc
     1861 ggttattttt tcttaaaatt ttacaaaagg ggcttattat gtctgaaatc tcaacgctcg
     1921 caccgcagct actttggcag tggtttgata aagcctgtgc tattccgcac ccgtcttacc
     1981 acgaaaatga actggctgaa tttattgtga attgggcaaa atcgaaaaat ttctttgttg
     2041 aacgtgatga agtgggtaat gtgttaatcc gtaaaccggc aactaaaggt atggaaaacc
     2101 gccaaactgt cgctctgcaa gcccatcttg atatggtgcc acaagccaat accggcacgg
     2161 ttcacgattt cactaaagat ccgattcagc cttatattga cggcgaatgg gtaaaagcca
     2221 aaggcacaac attgggggca gataacggca ttggattggc ttcgtgcttg gcggtgttgg
     2281 atagcgatga tttagcccac ccaaccattg aagtgttgct cacaatgacc gaagaagccg
     2341 gtatggaagg ggcgattggt ttacgcccga actggctaac cgccgatatt atgattaaca
     2401 ccgacaccga agaaaacggg gaaatttaca tcggctgtgc aggtggggaa aatgtgaatg
     2461 tgacactgcc aattttcttg gtgcctcatc agcaagatag tgcagtaacc gttacgctta
     2521 aaggcctaaa cggcgggcat tcgggctgtg atattcacac cacccgagcg aatgcgatta
     2581 aagtgttcgc ccgcattttg gaacaagccc acaacgaagt ggaattccaa attgcagaaa
     2641 ttcaaggcgg ttcggtgcgg aatgccattc cacgagaagc ccacgcaacc ttggttttct
     2701 ctgcaaaaaa tcagcaaaaa ttaaccgctt gtttaagcca aaccgccgaa caagtaaaag
     2761 cagaactcaa aattgccgag ccaaacttcc aatttttaat tgaagaagct caactgcctg
     2821 agcaagcctt tgaccttgcc agcactgctc gagtaatcca tttcttaaat gtgatgccaa
     2881 atggtgtggt gcgtaatagc gatgtggtgg aaaatgtggt ggaaacctca ctcagtattg
     2941 gtgtgcttaa caccgaagcg agtgaggtga aagcgaccat tcttgcccgc tcattaattg
     3001 agggcggaaa agaggaaatt cgcagtaaaa tccgctcatt aaccgcatta gtgggagcag
     3061 aagtggaatt ttcgggcaac tacccgggct gggagccaaa cccaaattcc aaaatcacgc
     3121 cattaaccaa agcgatttat gatgaaattt taggttatga atctcaaatt aaagtgattc
     3181 acgcagggct agaatgtggc ttaatcaaca aaatctaccc gaatatggat tttgtctcga
     3241 ttggaccaac tattctcaac gcccattctc cggacgaaaa agtgcatatt ccggcagtgg
     3301 agatttattg gaaactccta accaaactac ttgaacaagc acctgagaaa taaatttaat
     3361 caaaaaaatt atataatctt gccccgtaat taattgaata aaaccttaat taattacggg
     3421 gtagctctta ttttctctgt tttaaaaaat gtaccgtgaa agcagcaatt aataagacaa
     3481 ctgctgcacc taaataaagc tcgttctttt tccagccggc gtctaataaa atacctgcaa
     3541 gagttgggga aacaattgag actaaacgcc ctacaccgat ggcggtgcct acgcccgtgc
     3601 tgcggaaatc cggtgcgtat agggttgggt taatggtata aagcccggta atgcagccgt
     3661 taattaatcc gccaaccaaa atggctagtg ttatcgcaag ccctaaattg ccggcaaaca
     3721 caaaggcaac cacagcagat gcagccagaa tactgaatgc ctgcaaaata tgttttgccg
     3781 cccagcgact gactaaaaag ccgaaaatta acgagccgat tgtaccacca actgaaattg
     3841 ccattcccac agtttggctt tgggttttag gcattccggc ttcttctaat agtgccggag
     3901 tccaagagct gataaagtaa aatgaggctg aaattgcaat aaaggcaagc caaattagca
     3961 acgtattgcg gcgatattca ggctcgaata aacgggtaat tgccactttg cttttacttt
     4021 ttgcctcaat ttcaggaagt tcccagccgt ttcctaagcc taatttaacg gcaatttgat
     4081 ttaaacgagc tttagcattc accgggtgtt ttgaaattaa aaattccacc gattcgggca
     4141 atacaaagaa taataaaacg aataccgcac cggttaaaat cgacccaacg aggaaaaccg
     4201 cacgccagcc atattctcct tgcaataaca ccgcagacat tccgcccagc attgcaccaa
     4261 tcccaaagcc tgaggcataa attgcaatcg ctaagccacg ccatttttgg ctggagtatt
     4321 cactcactag cacattagtg ccggttaaaa tggcggctac gcctaaaccg gttaaaacac
     4381 gccatacacc aatttcaaac gcagaagtag cgaaataagt taggagcatt cctaacgtgg
     4441 ctaagcctgt gccgattaaa agcaacgggc ggcgaccaaa tttatcagca agtggtgaaa
     4501 gaaagagtga gcctatcgcc ataccgaata aaccggcact cattaagctg ccgatggctg
     4561 caccgtctaa ctgcaattct tctcggatgc ttttggcggt aaaggcgagt gccattaaat
     4621 caaagccgtc cagcatatta agtaacaccg taagcccaac cactaaccat tggaatgaag
     4681 tcattttgtt atgagcaatt ttttgacgaa tatccataag ctccctctgc atcaaaattg
     4741 ataaaatttt atttagagtg aactatagaa agttgattaa atataatcaa actcaaaatt
     4801 tacataaaat ttatgaaaga ttttcatatt taatcaaagg agggaattta tagcaggaat
     4861 ggcgagggag aaaatagata gggcgaataa ttattcaccc ctagattgaa taggagaaag
     4921 ctatttcttc tccgcttgct cagccgctct tttgcgtctg atttctttcg gatcagcaat
     4981 taacgggcga tagatctcaa ttctgtcgcc ttcttccacc gtttcattca gttttgccgg
     5041 gcggctaaaa ataccgactt tgttttcacg caagtcaatt tcggtatatt tttccaacac
     5101 gccggagagc aaaataatag tttgaatggt cgcgttttgc ggtacttgca gttttttcag
     5161 aaaatattta tcagggtaag cataaagcac ctcaacatta attttctgtt cttcactttt
     5221 ttgttcttct ggattttgac gttgttcaga caccatacac ctcttttgca cgttgtttga
     5281 aagcatttac catttttaaa gttaattcgt tgaacacctt gccgaacacc attgctacaa
     5341 ttgggctgga aaattcaaaa tttaattgca gggcaatttt gcaactttgc tcatcaaagg
     5401 gggtgaatgt ccaagctccg tgtaaatggc ggaaaggccc ttcaattaat gccatctcga
     5461 ttttataggg cggcgtcatt ttattgtggg tactgaaggt ttggctaatg cctaatttct
     5521 gaatatgtaa ctctgccttt aattcatttt cgccggtgct aagggttttg gtactcacgc
     5581 agccggataa aaattcgggg tatttttcat actgattaac aagttgatac atttgctcgg
     5641 cactataagg cacgagggca gattgattaa cgattggcat aatgtctctt ttggaaatta
     5701 acaagcggtc atattttagc gtaaatttgc aagcctataa agaaacactt ttgatttgaa
     5761 gatagacctc ttgccccggt tggattgcta attcattaaa agaccaaagg ctgatcgtac
     5821 tccaaatttg ctgattttcg acttgaattg ctaaatcaac ccgctccaaa tgcgtttcaa
     5881 ttttccaaac attgcctttt aacacattac gaattgagct ttgagtcggt ttttgtaagc
     5941 tgatcgaaac ctctttgctg gcaatagtca cccttagctt ctcgcctatt tgatggcgag
     6001 ggaattgctt cacccataat cgctgtgcgt tcaagctcaa gccaagcata ttatagtggg
     6061 gaagtgtttc ttccaccggt aattcaagca ggctaattcg ctgatgattt ggctgccaag
     6121 cggaaaatgc cgagctgtgc caaaccgctg aaagggtatc aaatgcggtt actttgccgt
     6181 tttccaacag cacaagatga tctgccaatc gaactacttc ctctaaactg tggctgacat
     6241 acagaatcgg tatttcaagc cgagcggata atttatctaa atagtccagt agctcttgct
     6301 tacgagggag atcgagggca gataatggtt cgtccatcag caaaatattc ggtgagctta
     6361 ataacgcccg cccaatcgcc acacgttgtt tttcgccacc ggaaaggctt gccggatagc
     6421 gattaagcaa aggggcaatg cctaacagtt ccacaatttg caaaaaatcc gcagaatccg
     6481 accgcttgta gccatatttt aagttttttt ccaccgaata atgagggaaa aggcgatgct
     6541 cttgaaacac atagccaatg ttgcgttttt caggtggtaa attgatgccg ttttggctgt
     6601 caaacagcac ctgaccgttt aattcgataa agccggcttg agcggttgaa agccccgcaa
     6661 ttaaattaat caggctggat ttaccggcac cggaacgccc gaaaatagcg gtgatccctt
     6721 tattcggcaa atcgagattg acattgagcg tcagattgcc gagttgctga agaagtttaa
     6781 gtttgagcat agcgtttttg cctttctgcc agccattccg aacagaatag ggcaatgagt
     6841 gaaatcagaa tcgccaccaa gcacaaacgg gcggcagcca tttcgccatc gggggtttca
     6901 ataaaggtga aaagggcggc aggaatggtt tgggtttggt tgggaatgtt ggagacaaag
     6961 gtgatggttg ccccaaactc ccccaaagag cgggcaaaac ccagcacgcc accggcaata
     7021 atgcctgaaa aagagagcgg taaggtgagc gtgaaaaata cttttaacgg tgtcgcccca
     7081 agcgttctgg cggcttgctc caatttagga tcgacggctt caaaagctaa acggatagaa
     7141 cgcaccatca gcggaaacgc catcaccatt gaggcaagca cagccccttt ccacgaaaaa
     7201 ctcaagctga aatgaaacca ttcccacaaa tattgcccga ttacccctcg ttttgccatc
     7261 gagatcagta acaaatagcc aatgacaacc ggcggtaaaa ccagagggag atgcacaata
     7321 ccattgagta gatttttgcc ccaaaactgt ttgcgggcaa gcagccacgc cacccacacc
     7381 gcaagcggta atgcaagcac tacggcaatg gaggcaactt ttaggcttaa aacaatcgca
     7441 ttgagttctt gtggggtgaa attaagcata atctaaaact attcggttat aagcttaccc
     7501 tcgccccttg tgggagaggg atagattttt c
//
LOCUS       DANKBV010000112         7420 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.112, whole genome shotgun sequence.
ACCESSION   DANKBV010000112 DANKBV010000000
VERSION     DANKBV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.112"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..218
                     /locus_tag="PWY43_001916"
     CDS             <1..218
                     /locus_tag="PWY43_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL2136775.1"
                     /translation="IYTDFLSREEIEFITQCKQKNLIKKQLNIMGIPFKENANGFPVV
                     RRDYAQAKQRKPIATNDSDWVSNAFKA"
     gene            240..1280
                     /locus_tag="PWY43_001917"
     CDS             240..1280
                     /locus_tag="PWY43_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2136776.1"
                     /translation="MARPRKYENNGLPQNLLCRRRKRGNGQIVEYYFYVLSNGKEKSL
                     GTNKYEAVLEAAKLNFENSKKSPVVLFIDVAKRYELEIVPTKKAKNTRQSNLQAIHWL
                     CKFFGNPPAPLEKIEPKHISQYLQWRKDTPAVANIEVGLFNTIWNMAREWGYTTLPSP
                     SQGVKKFPTKYREVYVEDYILDKIYEFADERMADIIETAYLLGQRPIDICNIHHSHIY
                     DGILHITQQKTGKKVRFEISGRLKEILDKRLGYESDWIFTNKWGKKLKRRSLGDHFKE
                     IKERAMKAYPELADEIAKVQMRDMRAKAATDISLMTTDEQAQKQLGHTSKRMTHHYIR
                     KDKILKPTDEIT"
     gene            complement(1439..1726)
                     /gene="rplY"
                     /locus_tag="PWY43_001918"
     CDS             complement(1439..1726)
                     /gene="rplY"
                     /locus_tag="PWY43_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDL2136777.1"
                     /translation="MSFKFEAEVRSAQGKGASRRLRHNGQVPAIIYGGNAEPVSIILD
                     HDKVNNAQVHDSFYNEVLTIVVAGKEEQVKVQAIQRHPTKPKLVHLDFKRA"
     gene            complement(1878..3146)
                     /locus_tag="PWY43_001919"
     CDS             complement(1878..3146)
                     /locus_tag="PWY43_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HDL2136778.1"
                     /translation="MSDLKQQALDFHEFPIPGKISVTPTKALDTQHDLALAYSPGVAE
                     PCLAIEKDPSASYRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAG
                     IDVFDIEINETDPEKLVEIIASLEPTFGGINLEDIKAPECFYVEQKLRERMGIPVFHD
                     DQHGTAIITGAAVINGLQVVGKAISEVRLVVNGAGASAIACTNLLRSLGVKKENITMC
                     DSKGVIFQGRGDKMDETKQFYAIEDNGWRTLADAVANADVFLGCSKGGALTQEMVKTM
                     AKDPLILALANPVPEITPAEAKAVRPDAIVCTGRSDFKNQVNNVLCFPFLFRGALDVG
                     AKTINEEMKMAAAHAIAELAKEPVPFEVISTYGELSFGPEYIIPTPFDPRLIVNVSIG
                     VAKAAIQSGVATRPITDWEAYAEQLKKLVA"
     gene            complement(3303..4214)
                     /locus_tag="PWY43_001920"
     CDS             complement(3303..4214)
                     /locus_tag="PWY43_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HDL2136779.1"
                     /translation="MKWLMLCREPRLYSCCRIKAACEAKGIELDILDPNRMLLGLENG
                     EFKIYYQAGESYDNNRPEPVLLPNYDGILPRFGTGSTEMGCSVLRHFEAKGIPVLNHS
                     AAFALARDKWRTLQKLAKHNLPVPNTNFAGHLVSVKSQLNQFAFPLISKVLNGSQGNG
                     VMLFEGKNNAEAVLATFRQVNEPYLCQQFVGEAKGQDIRAFVIGNEVVAAMSRTSVTG
                     DFRANIHQGGTAQPIELTQMERALAIKATKTIGLDIAGVDFLRTEKGVVILEVNASPG
                     FEGIERVNDVDIASEMVSYFINKMKNA"
     gene            complement(4211..4930)
                     /gene="nfsA"
                     /locus_tag="PWY43_001921"
     CDS             complement(4211..4930)
                     /gene="nfsA"
                     /locus_tag="PWY43_001921"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HDL2136780.1"
                     /translation="MQSKPTLETILSHRSIRKFTEQPISEEIFQTLIEAGKAASSSNH
                     LQCVTVIRVTDRQIRETLKAVSGMQYVVDCAEFLVFCIDFNKHKQLVPESQLEWAEVS
                     LIGAVDTGIFAQNVFLAAESLGLGAVYIGALRNDIERVSKALNLPEYCVPLVGMCLGY
                     PAQEPAIKPRLPNSLVCYNNQYQDFDWTEFAKYNENLTAYYQARIGEPQQWQAAIHKT
                     LDKPVRPHILPFFNQKGLLKK"
     gene            complement(4942..5727)
                     /gene="mtgA"
                     /locus_tag="PWY43_001922"
     CDS             complement(4942..5727)
                     /gene="mtgA"
                     /locus_tag="PWY43_001922"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDL2136781.1"
                     /translation="MEQSMAKRNKRFLSSLLKKIGLFFIPRKIKSKLGWLWFGSSRLL
                     TFGGTFFLIPILIFSVLPVPYSAYMVQKKVENLVQGKSYPIKKEWVSLNEISWQMQMA
                     VIASEDQKFESHFGIDLQAIETALKLNAKSKKVRGGSTISQQTVKNMFLWHGQSWLRK
                     GIEVPLTLLVENVWSKDRILEVYLNIAEFGNGIFGVEAAAKHFFKKSAKQLTLQESAL
                     LAASLPNPLVFRVDKPGPSMRKRQAWIMRQVSLLDGKNYLDKL"
     gene            complement(5702..6022)
                     /gene="trpR"
                     /locus_tag="PWY43_001923"
     CDS             complement(5702..6022)
                     /gene="trpR"
                     /locus_tag="PWY43_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDL2136782.1"
                     /translation="MKTLYNQRDPNEWTQFLALLEQAVAEDKLEPFFSMFLTADERGS
                     LGLRLQIVQALLKGEDSQREIQQNLNTSAATITRGSNMLKTLDQDFLQWVNGKVNGTV
                     NGKA"
     gene            complement(6102..6743)
                     /gene="lolA"
                     /locus_tag="PWY43_001924"
     CDS             complement(6102..6743)
                     /gene="lolA"
                     /locus_tag="PWY43_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDL2136783.1"
                     /translation="MKTLFKKSVFGLALFGLANVALADAQSVAELQRRLEQVSQYSAD
                     FDQTVRSSKGKQIQQGKGKFQVKRPNLFRMDTKSPQENLIVSDGKDLWFYDPFVSQVT
                     VNTVQDAVNNTPFVLLTSSDKSHWDQYDVSQSADTFVLKPKSKKSNLKQFDVRIDQSG
                     LLKGFSTIERDGQSNLYVLRNITTGGVSSDLFKFSVPKGAELDDQRGGKKSKK"
     gene            complement(6854..>7420)
                     /locus_tag="PWY43_001925"
     CDS             complement(6854..>7420)
                     /locus_tag="PWY43_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="has 3'-5' exonuclease, 5'-3' exonuclease and
                     5'-3'polymerase activities, primarily functions to fill
                     gaps during DNA replication and repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2136784.1"
                     /translation="EIFGVELGEVTSEQRRSAKAINFGLIYGMSSFGLSNQLGIGRAE
                     AQKYMDLYFQRYPAVQQFMTDIREVAVEKGYVETLFGRRLYLPDIKSGNAILRKAAER
                     VAINAPMQGTAADIIKVAMIGIDNAIRDNDEIKMIMQVHDELVFEVKADKIDHYSQLI
                     KTEMEKAISLKVPLIAEVGVGENWDEAH"
BASE COUNT         2251 a         1659 c         1332 g         2178 t
ORIGIN      
        1 acatctacac tgacttcctt tctcgtgaag agattgaatt tattactcaa tgcaagcaga
       61 aaaatctaat caaaaaacaa ctcaacatta tgggcattcc atttaaagaa aatgcaaatg
      121 gattccctgt tgtccgccgt gattatgctc aggctaaaca gcgtaaacct attgcaacga
      181 atgattctga ctgggtatca aatgcattta aagcgtaaat tgagaagaag gaggcactta
      241 tggcacgccc tcgaaaatat gaaaacaacg gattgccaca aaacctactc tgtcgtcgca
      301 gaaagcgtgg gaacgggcaa atcgtggaat actactttta cgttctctcc aatggcaaag
      361 aaaaatccct gggcacaaat aaatatgaag ccgttttaga agcagctaaa ctcaattttg
      421 aaaatagcaa aaaatcccca gtcgttttat ttattgatgt agctaaacgc tacgagctgg
      481 aaattgtccc taccaaaaaa gccaaaaaca ctcggcaatc taatttacag gctattcatt
      541 ggctatgtaa attctttggt aacccacctg caccgctcga aaaaattgag ccaaagcaca
      601 tcagtcaata tttacaatgg agaaaagaca cgccggctgt tgctaatatt gaggtaggtt
      661 tatttaacac aatttggaac atggcaagag aatggggata cacaacactt ccaagtccat
      721 cgcaaggtgt gaaaaaattc ccaactaaat atcgtgaggt ttatgtagag gattatatcc
      781 ttgataaaat ctatgaattt gcagatgaac gaatggcaga tattatcgag acagcgtact
      841 tgctaggaca aaggccgatt gatatttgca acatccacca ctcacacatc tatgacggca
      901 tactgcacat tactcagcag aagacaggga aaaaagtgag atttgaaatt agtggtcggc
      961 taaaagaaat tttagataaa agattgggat acgaatcaga ctggattttt accaataaat
     1021 ggggaaaaaa attaaaacgg cgaagtttgg gcgatcattt taaagaaata aaggagagag
     1081 caatgaaagc atatcctgaa cttgctgatg aaatcgctaa agtacaaatg cgtgatatgc
     1141 gtgccaaagc agccaccgat atttccctaa tgacaaccga tgaacaagct caaaaacaac
     1201 ttgggcatac atcaaaaaga atgacacatc attacatcag aaaagacaaa atcctcaaac
     1261 ctaccgatga aatcacctaa aaataaagcc cctttgccaa agacaaaaga gccatttaat
     1321 cgcctaaaat ccgttccgaa actattttgt aaataattga ttttattgac ctaaaaaact
     1381 ataaaatcgc aaaaagtttc ggaacagaaa acagcgttaa acccttttaa atcaagaatt
     1441 aagcacgttt gaagtctaag tgaactaatt ttggtttagt tgggtggcgt tgaattgctt
     1501 gtacttttac ttgctcttct ttacctgcaa caacgatagt taatacttcg ttatagaatg
     1561 aatcgtgaac ttgtgcgttg ttcactttat cgtgatctaa aatgattgat acaggctctg
     1621 cattaccacc gtagatgatt gcaggaactt gaccattgtg acgcaggcgg cggctcgcac
     1681 ccttaccttg cgcagaacga acttcagctt caaatttgaa tgacatagtt ttattctctt
     1741 aaataaaatg gggaaatccc cgtgaataga aaaaacagca ggcgacccag ctgtttccga
     1801 aaaaatatcg gcatatccgt ctcgatatgc cagctacgta ttttactgaa gttgcagaaa
     1861 aatgcaatgt aaaaatatta agcaaccagt tttttcaact gctctgcata agcttcccaa
     1921 tctgtaattg gtcgagttgc tacacctgat tgaattgctg cttttgctac accaatcgag
     1981 acattcacga tcaaacgtgg atcaaatggc gttggaataa tatattccgg gccaaacgat
     2041 aattcgccat aagttgaaat aacttcaaac ggcacaggtt ctttagccaa ctctgcaatc
     2101 gcatgggctg ctgccatttt catttcttca ttaatggttt ttgccccaac atctaatgct
     2161 ccacggaata agaacgggaa acaaagcaca ttattcactt ggtttttaaa gtctgaacga
     2221 ccggtacata caatcgcatc tggacggaca gccttcgctt cagcaggtgt aatttccggc
     2281 acagggttcg ctaaagcaag aattaatgga tcttttgcca ttgttttaac catttcttgc
     2341 gttaaagctc cgccttttga acaacctaag aaaacatctg cattcgcaac ggcatctgcc
     2401 aatgttctcc aaccattatc ttctattgca tagaattgtt tggtttcatc catcttatca
     2461 ccacgacctt ggaaaataac gcccttagaa tcgcacattg tgatattttc ttttttcaca
     2521 cctaatgaac ggagtaagtt agtacacgca atcgctgatg ctccagcacc gtttaccact
     2581 aaacgtactt ctgaaattgc tttacctacc acttgtaatc cgttaatcac cgccgcaccg
     2641 gtaataattg ccgtaccgtg ttgatcatcg tggaacacag ggattcccat acgttcacgt
     2701 aatttttgct ctacataaaa acattcaggt gctttaatat cttccagatt aatgccacca
     2761 aaagtcggtt ctaacgatgc aataatctcc actaatttct ccggatcagt ttcattaatt
     2821 tcaatatcaa acacatcaat tccggcaaat ttcttaaata gaacaccttt gccttccatc
     2881 acaggcttac ttgctaaagc accaatattg cctaagccta acactgctgt accattagaa
     2941 attactgcaa ctaagttacc tcgtgccgta taacgataag aagctgaagg atctttttca
     3001 atcgctaagc aaggctcagc aaccccagga gagtacgcta aagctaaatc gtgctgagta
     3061 tctaatgctt tagtaggagt aacggaaatt tttccgggga ttgggaactc gtgaaaatca
     3121 agcgcctgtt gttttaaatc gctcatttta gaaaccttat aatctataaa aataaaagta
     3181 atcaataata gccaaagagt agccatttta ccctaaattt tttcgcattt ttatggcttt
     3241 gctctcattt tttatgatat ttacaaaaaa taatacaaaa acgtccgctt gttttactca
     3301 aatcaagcat tcttcatctt attgataaaa taagaaacca tttcagaagc tatatcaaca
     3361 tcatttactc tctctatgcc ttcaaatcct gggctggcat tgacttctaa aataactacg
     3421 cctttctctg tccgcagaaa atcaacgcct gcaatatcaa gccctatcgt tttagttgct
     3481 ttaattgcta acgcacgttc catctgagtt aattcaatcg gttgagccgt tccaccttga
     3541 tgaatattgg ctctaaaatc acctgttacg ctggttcggc tcatggcagc aaccacttca
     3601 ttgccaatca caaacgcccg aatatcttgc cctttagctt cgcccacaaa ttgttggcat
     3661 agataaggct catttacttg cctaaaggtc gctaacacgg cctcggcatt atttttgcct
     3721 tcaaatagca ttacgccatt cccctgtgaa ccatttagca cttttgaaat aagtggaaaa
     3781 gcaaattgat tgagttgtga tttcacagaa accaaatgcc ctgcaaaatt cgtattagga
     3841 accggcaaat tgtgtttggc taatttttgt aatgttcgcc atttgtctcg ggctaacgca
     3901 aatgccgctg aatgatttag aacaggaata cctttcgcct caaaatggcg taacaccgaa
     3961 cagcccattt cggtactgcc tgtgccaaaa cggggtaaaa tgccgtcata attcggcaac
     4021 aataccggct ccggacggtt gttatcatag ctctcgccag cttgatagta gatcttaaat
     4081 tcgccatttt ctaatccgag tagcattcga ttcgggtcta aaatatccag ctcaatcccc
     4141 tttgcctcac aagcggcttt tattcgacag cagctatata aacgaggctc tcgacatagc
     4201 attagccact tcattttttc agcaatcctt tctgattgaa aaacggcaaa atatgcggac
     4261 gaacaggctt atccaatgtt ttatgaatag ctgcttgcca ttgttgtggc tcgccgattc
     4321 tggcttggta gtaagcggtc aaattttcat tatattttgc aaattcagtc caatcaaaat
     4381 cctgatattg attgttataa caaaccaaac tattcggcaa acgaggtttt attgccggct
     4441 cttgtgccgg ataccccaaa cacataccca ccagtggaac acaatattcc ggcaagttaa
     4501 gggctttaga aacccgctca atatcattac gcaatgctcc aatatacacc gctcccagcc
     4561 ctagtgattc ggcggctaaa aatacattct gggcaaaaat accggtatct accgccccaa
     4621 tcaacgacac ctccgcccat tccaactgcg attcagggac aagttgtttg tgtttattaa
     4681 aatcaatgca gaacaccaaa aattcagcac aatctactac atattgcata cctgaaacgg
     4741 cttttaatgt ttctcgaatc tgccgatcgg ttacacgaat caccgtcaca cattgcagat
     4801 gatttgaact tgaagccgct ttacctgcct caattaaggt ttggaaaatc tcttcagaaa
     4861 taggctgttc ggtaaattta cgaatagaac ggtgagataa aatcgtctca agagtcggtt
     4921 tactttgcat atttttctcg tctaaagttt atctaaataa tttttgccat caagcaaact
     4981 tacttgtcgc ataatccacg cttggcgttt tcgcatactc gggccgggtt tatctaccct
     5041 aaacaccaac gggttaggca aagaggctgc caatagtgcg gattcttgca aggttagctg
     5101 tttggctgat tttttgaaaa aatgctttgc ggctgcttcc accccgaaaa tgccgttacc
     5161 aaattccgca atattgaggt acacctctaa aatccgatcc ttactccaca cattttcgac
     5221 taacagggtt aaaggcactt caatcccttt gcgtaaccag ctttgcccgt gccacaaaaa
     5281 catatttttt accgtttgtt gcgaaatggt tgaacctcca cgcacttttt tggatttagc
     5341 attcagtttc aaggcagttt ctatcgcttg taagtcaatg ccaaaatggc tttcaaactt
     5401 ttgatcttct gaggcaatca cagccatttg catttgccac gaaatctcat tcaggctcac
     5461 ccattctttt ttgatcgggt aagatttccc ctgaaccaga ttctccacct ttttttgcac
     5521 catatacgca gaataaggca ccggtaacac cgaaaaaatc agtatcggaa tcaggaaaaa
     5581 tgtgccacca aaggttaata agcgactaga accaaaccaa agccagccca gtttactttt
     5641 tattttgcga ggaataaaaa aaagccctat tttcttcaaa agagagctta aaaatcgctt
     5701 gttacgcttt gccattgact gttccattaa ccttgccgtt cacccattgc agaaaatctt
     5761 ggtcgagtgt tttaagcata tttgaaccac gagtgatggt tgccgcactg gtatttaaat
     5821 tttgttggat ttcccgttgc gaatcttcac cttttagcaa agcttgcaca atttgtaaac
     5881 gcaagcctaa tgaacctcgc tcatcggcgg tgagaaacat tgagaaaaac ggctcaagtt
     5941 tatcttccgc aaccgcttgt tcaagcagag ccaaaaactg cgtccactcg tttggatctc
     6001 gctggttata gagtgttttc atactgtccc ttgaagtaaa aacggtgaca agcggtcaga
     6061 tttttacaaa attttgcaga aaaacgaccg cttgctctcc attatttttt cgatttttta
     6121 ccgccacgtt ggtcgtctaa ctctgcccct ttcggtacgc tgaatttaaa caaatcgctt
     6181 gagacaccgc cggtagtaat attgcgtaac acatataagt tactttgccc gtcacgctca
     6241 atggtgctga aaccttttaa taaaccgctt tggtcaatcc gcacatcaaa ctgttttaaa
     6301 ttgctttttt tcgattttgg cttaagcacg aaagtatcag cactctggct gacatcatat
     6361 tgatcccagt ggcttttatc actgctggtt aataagacga atggcgtatt attaactgca
     6421 tcttgcacgg tatttaccgt aacttgtgaa acgaaaggat cgtaaaacca taaatctttg
     6481 ccgtcagata caatcaaatt ttcttgtggt gatttggtat ccattctaaa taagttcggg
     6541 cgttttactt ggaatttgcc tttgccttgc tgaatttgtt tacctttgct agaacgcacg
     6601 gtttggtcga aatccgcact atattggctt acttgttcca aacggcgttg taattctgca
     6661 acagattgag catcagccaa tgccacattt gccaagccaa ataacgctaa accgaatacc
     6721 gattttttaa ataatgtttt catttgtttt cctttttacc aacaagaatt ttcttgtttg
     6781 acaataaaat catcaaaagt ttcatattaa tgaactgtta caacaataca ataaaagtcg
     6841 tgaattgtat agtttaatga gcttcgtccc agttttcgcc tacgccaact tcggcaatca
     6901 gtggcacttt gaggctaatc gctttttcca tttcggtttt gattaattgg ctgtaatggt
     6961 cgattttatc ggctttgact tcaaacacca attcatcgtg aacctgcata atcattttga
     7021 tttcatcatt atcacgaatc gcattatcaa taccaatcat cgccacctta ataatatcgg
     7081 cagccgtgcc ttgcattggg gcgttgattg ccacccgttc tgctgcttta cgcaaaatag
     7141 cattgccgga tttaatatca ggcaagtaca aacggcgacc aaatagggtt tcaacatagc
     7201 ctttttcaac tgcaacttca cgaatatcgg tcataaattg ttgaaccgcc gggtaacgtt
     7261 ggaagtagag atccatatat ttttgagcct ctgcacgccc aatccctaat tggtttgaaa
     7321 gcccaaatga cgacattccg taaatcagcc cgaagttaat tgctttcgca ctgcggcgtt
     7381 gttcggaggt gacttcacct aactccaccc cgaaaatttc
//
LOCUS       DANKBV010000113         7302 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.113, whole genome shotgun sequence.
ACCESSION   DANKBV010000113 DANKBV010000000
VERSION     DANKBV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.113"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..93)
                     /locus_tag="PWY43_001926"
     CDS             complement(<1..93)
                     /locus_tag="PWY43_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase N-4/N-6"
                     /protein_id="HDL2136785.1"
                     /translation="MTTLTWYGKEEAVKKAKQTPYCLLRELPDFS"
     gene            complement(234..2720)
                     /gene="torA"
                     /locus_tag="PWY43_001927"
     CDS             complement(234..2720)
                     /gene="torA"
                     /locus_tag="PWY43_001927"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL2136786.1"
                     /translation="MKKQDSIDVSRRGFLKNSSLSLAAGAGLASGTTGVVGALASTSA
                     KAAEMKTVVTAAHWGPLGVVVENGKVVKSDSVFEISVPNELQHVVADQVHGETRVKYP
                     MVRKGYLEGNKDTTIRGRDEWVRVSWDKAFELVANEMKRVREAHGANAIFGGSYGWYS
                     SGALHASRTLLQRYLNVTGGFVGVKGDYSTGAAQVIMPHVLGTIEVYEQQTSWEVILE
                     SSDIVVLWSANPLNTLRIAWTSTDQQGIEYFKKLKESGKRIICIDPVKSESCEFLGAE
                     WVPVNTGTDVALMLGMAHTLVAEGKHDQAFLKKYTTGFEKFEEYLLGKADNQPKDAKW
                     ASQICGVPEETIKKLATDFQSKRTMLMAGWGIQRQRHGEQGHWMLVTLAAMLGQIGLP
                     GGGFGLSYHYSNGGVPSATGGILGSITANPAVEAGAKTWLDETSKSSFPVARISDALL
                     NPGKTIDYNGTQITYPDIKIVYWAGGNPFTHHQDTNTLVKAWQKPDTIIVNECHWTPT
                     ARMSDIVLPATTSYERNDLTMSGDYSMMNIFPMKQVVEPQFEAKNDYDIFAELAKRAG
                     KEAEFTEGKSEMDWLQSFYQTAFEAARKNRVIMPKFEKFWQENKPLSFKASDKAKKWV
                     RYEEFRNDPLLNPLGTPSGKIEIFSEVIAKMNYDDCKGHPTWMEHEEYSGKTTAEEPL
                     ALVTPHSKWRLHSQLAYSSLRKQYAVNDREPVLIHTEDAAARGITSGDIVRIFNKRGQ
                     VLAGAVVTDGIIKGTLALHEGAWYDPLDLGTSEQPLCKNGNPNVLTRDEGTSKLAQGN
                     SPNTATVQVEKFTGQAPEVTVFKEPKYTQG"
     regulatory      complement(2739..2934)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(2938..4053)
                     /locus_tag="PWY43_001928"
     CDS             complement(2938..4053)
                     /locus_tag="PWY43_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL2136787.1"
                     /translation="MAKMKKIAVFVGIMALGAAVLGGTQYAMKATSSTEFCVSCHSMT
                     HPQAEWEGSVHFSNRKGIRAECSDCHLPQDGLHYVKAKIIALKDVWHTVVTNKLPDQE
                     AYEAHRLEMAQRVWAEMKENDSMTCKSCHSFDAMVLSEQTEAAQKMHKLAQETNQTCI
                     DCHKGIAHFMPDVPVDNAAAMGELAKHGAEFSTNDKTLYTLAMTPVQLAQGGEIRLLP
                     YAELHQWTAEGEQVKATVNGWQQEGAESVVYMDLGKRIMVALLPDDAQSKVNVTKTVF
                     DNVTNSNWKEINFEITAPKNAVTANITALNHFGDNLNQTHCSGCHAPIGAEHYTANQW
                     IGVVNSMKDRTSMSADDVRAVTIYLQRNAKDVAGSSH"
     gene            complement(4195..5946)
                     /locus_tag="PWY43_001929"
     CDS             complement(4195..5946)
                     /locus_tag="PWY43_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2136788.1"
                     /translation="MDKRSRWSCLVNEQGEISEWEDKAGADYFAELPQICHYAQALLY
                     SDKNSLQFYYSSNHQWLKISLERHQSFVLTQAEHTDLPYGLSKREIELLTLISSGLSN
                     EEIANELCISKRTVDKHIENIFAKTHTESRTLLAVFAITEHLVCLPTPGKLTQSILAS
                     FNIEQLALQIIAKKQPLVTPSTRHELPMFTISHHHTRPIIIGVPYVEQGIGQIDTREL
                     LNGTQLAVETINQQGGIHGREVQIATAGFCVKDKASILQAYHHLFDQEVDAISTAYAC
                     YLPEVHELIATKGIPYLHIATHSGSNKLAQNLPNQRIENMFQVCASDVTYGLGISHFL
                     QYYRYHYAPWLQNKTVMVVTVSWQKVDIGIESLTLSLREQGWNVDYLELTQEQDNYAK
                     LMTQVHNLTPSIIVFASYFAEDIVQFYQTFIRNPTNAIIYSIYAPSAFLPHEKLCEGV
                     LWSTNSGLSENYLGRQFSQHYEQFFGKSPTYSQASIAYDQANILANAWKQSVSPRHFK
                     AVSNAIRLQPHYGVNGTYYFNTDSQIGLTYAETHDLSISLPQLVYQIQQGQSRVIAPE
                     LFANAQFILPPWFSEKA"
     regulatory      6110..6266
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            6255..7280
                     /gene="moaA"
                     /locus_tag="PWY43_001930"
     CDS             6255..7280
                     /gene="moaA"
                     /locus_tag="PWY43_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDL2136789.1"
                     /translation="MQSIIPIKNVGDDVVSGLVDRFQRQYTYLRLSITDVCNFRCNYC
                     LPDGYKPPSHKQTFLSVDEIQRVAKAFANLGTEKIRITGGEPTLRKDFLEIAHTISQT
                     PGIRKVALTTNGYRLERDVELWQQAGITDINVSVDSLDPRQFQLITGENKLQSILKGI
                     DKAFEIGYPKIKVNAVLMKQYTAPELDKFLTWIKDKPIQMRFIELMETGEMDSFFQAQ
                     HLSGQSVMTRLLQEGWQLQPKAMSDGPAKVLSHPDFKGEIGLIMPYEKNFCASCNRLR
                     VSALGKLHLCLFGEEGIDIRDLLASDEQQLQLETRLKSSLQGKREHHYLHIGDSGVRN
                     NLASIGG"
BASE COUNT         2002 a         1578 c         1494 g         2228 t
ORIGIN      
        1 cgagaagtcc ggcaactcac gcaataggca atatggggtt tgttttgctt ttttgacggc
       61 ttcttctttg ccgtaccaag taagggttgt cataaaatgc acctgaaaag taaaagtgga
      121 ttattgtaac aagcggtcaa aaaaatagaa agttttacaa aaaattaagc aaatttaacc
      181 gcttgcaaca tagaaaaggc acagttttat actgtgcctt tggttatatg aaattagcct
      241 tgtgtgtatt taggctcttt aaatactgtt acttctggtg cttgtccggt gaatttttca
      301 acctgtaccg tagcggtatt tggtgagtta ccctgtgcta attttgatgt gccttcatca
      361 cgggttagca cgttagggtt gccgttttta catagcggtt gctcgctggt gccgagatcg
      421 agcggatcgt accaagctcc ttcgtgcaag gctaatgtac ctttgataat gccatctgtc
      481 accactgcac cggctaatac ttgaccacgt ttgttaaaaa tacgcacaat atcaccgctt
      541 gtaatgccac gagcggcggc atcttcagta tggatcaaca ccggctcacg gtcattaacc
      601 gcatattgtt tgcgtaatga tgagtaagct aactggctgt gtaaacgcca tttagagtgt
      661 ggagtcacca atgccaacgg ttcttctgcg gtggttttac ccgagtattc ttcgtgttcc
      721 atccaagttg ggtgaccttt acagtcatca taattcattt tggcaatcac ctcagagaaa
      781 atctcaattt tgccggacgg tgtgcctaat ggatttagga gtgggtcatt acgaaattct
      841 tcataacgaa cccatttttt cgctttgtct gatgctttaa aagaaagtgg tttattctct
      901 tgccagaatt tctcgaattt cggcataatc acacgatttt tgcgtgctgc ctcaaatgca
      961 gtttgataga aactttgtag ccaatccatt tcagatttgc cttcggtaaa ttccgcttct
     1021 ttgcctgcac gtttggctag ttcggcaaaa atatcgtagt cgttttttgc ctcaaattgc
     1081 ggttcgacca cctgtttcat cgggaaaata ttcatcattg aataatcgcc cgacatagtt
     1141 aagtcattac gctcatagct ggtggttgcc ggcagcacga tgtcagacat acgagccgtt
     1201 ggtgtccaat ggcattcatt gacgataata gtatccggtt tttgccacgc tttcaccaaa
     1261 gtgttggtgt cttggtggtg ggtaaacgga ttaccccctg cccaatagac aattttgata
     1321 tcaggataag taatttgtgt gccattgtaa tcaatggttt tgcccgggtt gagcagtgca
     1381 tcggaaatac gagccaccgg gaatgaagat tttgatgttt catctaacca ggttttcgct
     1441 ccggcttcta ctgccggatt tgctgtgata gagcctaaaa tgccccctgt ggcactcggc
     1501 acaccgccgt ttgagtaatg gtagcttaaa ccgaaaccac cacccggtaa gccgatttga
     1561 cctaacattg cagctagggt gactaacatc caatgccctt gttcaccgtg tcgttggcgt
     1621 tgaatccccc aacctgccat taacattgtg cgtttagatt ggaaatcagt ggctaatttt
     1681 ttaatggttt cttctggcac accacaaatt tgacttgccc attttgcatc tttcggttga
     1741 ttgtctgcct taccgagcag atattcttca aacttctcaa aaccggtggt gtatttttta
     1801 aggaatgctt gatcgtgttt gccttcagcg actaaggtat gagccatacc gagcattaac
     1861 gcaacatccg tgccggtatt taccggtacc cattctgcac ctaaaaattc acaagattcg
     1921 ctttttaccg ggtcaataca gataatgcgt ttcccgctct ctttgagttt tttaaagtat
     1981 tcaatacctt gttgatcggt tgaagtccac gcaatgcgta gcgtatttaa cgggttagct
     2041 gaccaaagca ccacaatatc tgaactttct aaaatcactt cccaactggt ttgctgttcg
     2101 tacacctcga ttgtacctaa aacgtgtggc ataattactt gagctgcacc ggtagaatag
     2161 tcgcctttta cgccaacaaa tccgccggta acatttaaat agcgttgtaa cagtgtacgg
     2221 gaagcgtgta atgccccaga actataccaa ccgtaagaac caccaaaaat agcattcgcc
     2281 ccgtgagcct cacgaacacg tttcatttca ttcgcaacta attcaaatgc cttatcccac
     2341 gaaactcgca cccattcatc acgcccacgt atagtagtat ctttattacc ttctaagtag
     2401 cctttacgca ccattgggta ttttactcgt gtttcaccgt gtacttggtc tgctaccacg
     2461 tgttgcaatt catttggcac agaaatctca aaaacagaat cagatttcac cactttgcca
     2521 ttttctacca ctacgcctaa aggcccccag tgggcagcag ttactactgt tttcatctcg
     2581 gcagctttgg cactggtgga agctaatgcc cccactacac cggtagtacc actggctaaa
     2641 cctgcaccgg cagctaagct taatgatgaa ttttttagaa aaccgcgacg acttacgtca
     2701 atactgtctt gttttttcat aataaacctc gtttgaagtt aagttaaaaa tagacacctt
     2761 tctaaagtcg agtggataaa ccatttctcg tgtatccatt ggtaaagaac cgacggtaat
     2821 ttttgcaaaa tgttaggaaa aaatgaccgc ttgtaatagg tgctttacgg cttgctacca
     2881 tatctctgct atcagtagac acttaggctc acaagctcgg tatcctctaa gttacaatta
     2941 atgggaactg cctgctacat cttttgcatt acgttggaga tagatagtca ccgcacgcac
     3001 atcatcagcc gacatagatg ttctgtcttt cattgaattt accaccccaa tccattgatt
     3061 tgcggtgtaa tgttctgccc caatcggtgc atggcaaccg ctacagtgtg tttggtttaa
     3121 gttatcgcca aaatgattta gtgcagtaat attggcagtg actgcatttt taggagcagt
     3181 aatttcaaaa ttgatttctt tccaatttga gttggtgaca ttatcaaaca ctgtttttgt
     3241 gacattcact ttgctttgag cgtcatcagg tagaagagca accataatac gcttgcctaa
     3301 atccatatac accacagatt ccgcaccttc ttgttgccag ccattaaccg ttgctttaac
     3361 ctgctcgcct tctgctgtcc attgatgtaa ttctgcataa ggtaacaagc gaatttctcc
     3421 accttgagcg agctgcactg gagtcattgc gagcgtataa agggttttat catttgtgct
     3481 aaattccgca ccgtgtttag ctaattcgcc cattgctgcg gcattatcta ccggcacatc
     3541 aggcataaaa tgggcaattc ctttatggca atcaatacag gtttgattag tttcttgggc
     3601 tagtttgtgc attttctgtg ccgcttctgt ttgttcagaa agtaccatcg catcaaaact
     3661 atgacagctt ttacaggtca tagaatcgtt ttctttcatt tctgcccaaa cacgttgtgc
     3721 catttctaga cgatgggctt cataggcttc ttgatcaggt aatttattgg ttacaacggt
     3781 gtgccaaaca tcttttaatg cgatgatttt agctttgaca taatgcaagc cgtcttgtgg
     3841 tagatggcaa tccgaacact cggctcgaat tcccttacgg ttggaaaaat ggacactgcc
     3901 ttcccactct gcctgtgggt gagtcataga atggcagctc acacaaaatt ctgttgagct
     3961 ggtggctttc attgcatatt gtgtgccacc taataccgca gcacctaatg ccataatacc
     4021 gacaaataca gcaatttttt tcatcttagc cataaattac cccacttaat acactaaaaa
     4081 ttaaagacag aggcattgta ttaactccca agaggtataa aaataagtaa aaatacctat
     4141 tacctcgaaa gagtgagaat aatttgatct aaggcaaaaa tatgtagtga aattttaggc
     4201 tttttcagag aaccacggcg gtaagataaa ttgtgcattg gcaaacaact ccggtgcaat
     4261 cacacggctc tgcccttgtt ggatttggta aaccaactgt ggcaagctga tagataagtc
     4321 gtgggtttct gcgtaagtta atccaatttg gctatcggta ttaaaatagt aagtgccgtt
     4381 taccccataa tgtggctgta agcggatcgc attagagacg gctttaaaat gccgaggtga
     4441 aacactttgt ttccacgcat ttgctagaat atttgcttgg tcataagcaa tactggcttg
     4501 ggaataagtg ggcgatttgc cgaaaaattg ttcgtaatgt tggctaaact gtctgcctaa
     4561 ataattttcg cttaacccac tattggtact ccagagaacg ccctcgcaaa gtttctcgtg
     4621 tggcaaaaac gccgaaggtg cataaatact gtaaataatg gcatttgttg ggttgcgaat
     4681 gaacgtttga taaaactgta caatatcttc tgcaaaataa gaggcaaaca caataatgga
     4741 tggcgtgaga ttatgcacct gtgtcattaa tttggcataa ttatcttgct cttgggtgag
     4801 ttccagataa tccacattcc agccttgctc ccgcaaactt agggttaaac tttcaatacc
     4861 gatatcaact ttttgccaac tcaccgttac caccatcacc gttttattct gaagccaagg
     4921 ggcataatgg taacggtaat attgcagaaa gtgagatata cctaagccat aggttacatc
     4981 actggcgcaa acctgaaaca tattttcaat tcgttgattc ggtaaatttt gggcaagttt
     5041 atttgagcca ctatgagttg caatatgcaa ataaggaatg cctttagttg caattaattc
     5101 gtgtacttcc ggcaagtagc acgcataagc ggttgaaatg gcatcaactt cttgatcaaa
     5161 taaatgatgg tatgcctgaa gaatacttgc tttatcttta acgcaaaacc ctgccgttgc
     5221 aatctgcact tctctaccgt gaatcccccc ttgttgattg atggtttcaa ccgcaagttg
     5281 agtgccgtta agtaactctc gggtatcaat ttgcccaata ccctgttcaa cataaggcac
     5341 gccaataatg atcgggcgag tatgatggtg gcttatggta aacatcggta gttcgtgccg
     5401 agttgaaggc gttacaagcg gttgtttttt cgcgataatt tgcaatgcca gttgttcaat
     5461 gttaaagctg gcaagaatgg attgggttag ctttcccggt gtaggcaaac agactaaatg
     5521 ttcggtaata gcaaaaaccg ctaataaggt tcggctttcg gtgtgtgttt ttgcaaaaat
     5581 attttcgata tgtttatcaa ctgtccgttt gctgatacaa agctcattgg cgatttcctc
     5641 attgctcaaa ccactgctaa ttagagtgag aagctcaatc tcccttttac tcagtccata
     5701 aggcaaatcg gtatgctctg cttgagtcaa tacaaacgat tggtggcgtt ctaggctgat
     5761 cttaagccat tgatggtttg aactatagta aaactgcaag ctatttttat ctgaatagag
     5821 caatgcctgt gcatagtggc aaatttgcgg taattcagca aaatagtctg cccctgcttt
     5881 gtcttcccat tctgatattt cgccttgttc atttactaaa caagaccatc ggctacgttt
     5941 atccattctc acctcgaact aaaaacgtta gctaaaatag caaaattatc tcggcattaa
     6001 tatgatataa atcaaactaa gcaacggttg ccagattttt ataggtagag catacaattt
     6061 aaattatacc tcttgcctaa ttaggtttct caccataaaa tgaaaaccaa tttattgaag
     6121 actccgagca ttatttccta actctaaaaa ttaaagacaa atttttgata tggaatttat
     6181 gccagtaaca gcataaagct gttcaaagga taacttagaa atgggttgtc ctctcgtgtt
     6241 tagaaaggtg tcaaatgcag tctattatcc caattaaaaa cgtgggtgat gatgtcgttt
     6301 caggtttagt tgatcgcttt cagcgtcaat atacttattt gcgactatcc attactgatg
     6361 tttgtaactt ccgttgtaat tattgcttgc ctgatggcta taagccgcct tcacataagc
     6421 aaacttttct tagcgttgat gaaatccagc gggtggctaa agcctttgcc aatttaggga
     6481 cggaaaaaat ccgtatcacc ggtggtgagc caactttacg caaagatttc ctcgaaatcg
     6541 ctcacactat ttcacaaaca cccggtatcc gtaaagttgc cctaaccacc aacggctatc
     6601 gcctagagcg tgatgttgaa ctgtggcaac aagccggtat taccgatatt aatgtgagtg
     6661 ttgatagcct tgacccacgc caatttcaac tgattaccgg cgagaacaag ttgcaatcta
     6721 ttttaaaggg tattgataaa gcctttgaaa ttggctaccc aaaaattaaa gtcaatgcgg
     6781 tgctaatgaa gcaatatacc gcccctgaat tggataaatt cctcacttgg atcaaagaca
     6841 agccgattca aatgcggttt attgagctga tggaaaccgg tgaaatggat agtttcttcc
     6901 aagctcaaca tctttctggt caatcggtga tgacccgctt attacaagaa ggctggcaat
     6961 tacaacccaa agcgatgtct gacggcccgg caaaagtgct ttctcaccct gattttaaag
     7021 gcgaaatcgg cttaattatg ccctacgaga aaaatttctg tgcctcttgt aaccgcttgc
     7081 gtgtttccgc acttggtaaa ctgcatcttt gcttatttgg tgaagaaggg attgatattc
     7141 gtgatttact ggcaagcgat gagcaacagt tacagcttga aacccgccta aaatcttcct
     7201 tacaaggcaa gcgagaacac cactatttgc atattgggga tagtggggtc aggaataatt
     7261 tggcgagtat cggtggttaa acaaccaccg agcgtgtttt tt
//
LOCUS       DANKBV010000114         7139 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.114, whole genome shotgun sequence.
ACCESSION   DANKBV010000114 DANKBV010000000
VERSION     DANKBV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.114"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..671)
                     /gene="xseA"
                     /locus_tag="PWY43_001931"
     CDS             complement(<1..671)
                     /gene="xseA"
                     /locus_tag="PWY43_001931"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2136790.1"
                     /translation="MSNILTVTQLNYSVRHLLEMEMSHIWLTGEISNFSQPVSGHWYL
                     TLKDDKAQVRCAMFKMKNVRVNFRPQNGMQVLVRASVTLYEPRGDYQLIIESMQPAGE
                     GLLQQQFEQLKAKLSAQGLFAQEHKKPIPTFVKTVGIITSSSGAALQDILHILQRRDP
                     SLSVVIYPALVQGKEATQDIVNMIELANRRNECDVLIVGRGGGSLEDLWCFNEESVAY
                     AIYHS"
     gene            complement(822..898)
                     /locus_tag="PWY43_001932"
     tRNA            complement(822..898)
                     /locus_tag="PWY43_001932"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(862..864),aa:Met,seq:cat)
     gene            1108..2214
                     /gene="mltA"
                     /locus_tag="PWY43_001933"
     CDS             1108..2214
                     /gene="mltA"
                     /locus_tag="PWY43_001933"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDL2136791.1"
                     /translation="MNWKAYKKAIAAASVVAIATVLVACSSSGQKGKTTSYDAEKAKF
                     GAKYNGRHFMPYGTHFQAGRLGLNAAPVNQLDFLQQLSNVRAYSSLADSYAIAYGKIS
                     RWISSGGNINDLAKYGISTRLMRGEDGYQNVLMTGYYSPVIKARRTPQGQYQHPIYAM
                     PSSKRFSRAEIYAGALQGQGLELAYSNSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQ
                     NGFKYASVGRLLVEDGEIPKEKMSVQAIREWGQRNPSRVQGLLERNPSYVFFKNDPTG
                     QVKGSAGVPLVPLASVASDRSIVPSGSVLLVEMPLIDSKGNWTGKHEMRLMVALDVGG
                     AVKGHHFDLYQGIGDKAGHIAGLMKHYGRVWVLN"
     gene            2285..2689
                     /locus_tag="PWY43_001934"
     CDS             2285..2689
                     /locus_tag="PWY43_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136792.1"
                     /translation="MLANNLQNITASTEPKYKISEIDVRILIRQLNNIEQCIYPELAK
                     PGYQQIYANWNLAENLTMQYFEYQLLKELLGEENQKLMQNDTLSTEYFHLLHSRLNHQ
                     KANVDPEKCDTFKPRYKEIYKSMENALTKKNQ"
     gene            complement(2723..4240)
                     /gene="purF"
                     /locus_tag="PWY43_001935"
     CDS             complement(2723..4240)
                     /gene="purF"
                     /locus_tag="PWY43_001935"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2136793.1"
                     /translation="MCGIVGIIGNSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFR
                     LRKANGLVSDVFQQEHMVRLQGNVGIGHVRYPTAGSSSVSEAQPFYVNSPFGITLVHN
                     GNLTNNAELKARLYNEARRHVNTNSDSESLLNIFAYFLDLYSTQHLSPDNIFETVRKT
                     NDSIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKREIEGKTEYMFASESVALDVVGF
                     EFVRDVLPGEAIYVTFDGQLHSQICADNPKLNPCIFEYVYFARPDSVIDGVSVYSARV
                     HMGELLGEKIKREWGRIIDDIDVVIPIPETSNDIAVRIANMLYKPYRQGFVKNRYVAR
                     TFIMPGQAQRKSSVRRKLNAIASEFKGKSVLLVDDSIVRGTTSEQIVEMARAAGAKRV
                     YFASAAPEIRYPNVYGIDMPTCEELVAYDRSVEEVAQMIGVDKLIFQDLEALYKSIQL
                     ENPTIHRFDDSVFTGEYITGDVDKCYLDSIARSRNDKAKAEAAKQATNLEIHNER"
     gene            complement(4255..4758)
                     /locus_tag="PWY43_001936"
     CDS             complement(4255..4758)
                     /locus_tag="PWY43_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL2136794.1"
                     /translation="MVDLIVIGIIAFSVLISLWRGFTNEALSLLGWVIAFFVASSFYP
                     YLSGYLTQINSVYLQNSEFLRNGIAAAILFILTLIVCGVINGLLGKVIDLSGMSITDR
                     ILGGAFGALRGILIVSAILFFLDTFTQASQTELWKSSLLIPHFDFIVKWFFEQLQANS
                     SFLNTTK"
     gene            complement(4904..5365)
                     /gene="rnhA"
                     /locus_tag="PWY43_001937"
     CDS             complement(4904..5365)
                     /gene="rnhA"
                     /locus_tag="PWY43_001937"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDL2136795.1"
                     /translation="MKLVEIFTDGSCLGNPGKGGIGILLRYNGYEKTVSKGYFQTTNN
                     RMELRAVIEALAMLKEPCKVQLNSDSQYMKNGIQKWIFNWKKNDWKTSDKKPVKNKDL
                     WVALDQEIQRHQIEWSWVKGHSGHRENEICDELAKQGANNPTLDDVGYIAD"
     gene            complement(5458..6789)
                     /gene="argG"
                     /locus_tag="PWY43_001938"
     CDS             complement(5458..6789)
                     /gene="argG"
                     /locus_tag="PWY43_001938"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005722481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDL2136796.1"
                     /translation="MSATILEALPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTA
                     NLGQPDEDDYNAIPKKAMEYGAENARLIDCRQQLAHEGIAAIQCGAFHISTGGIPYFN
                     TTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
                     QLFIDELGGRLEMSKFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIV
                     KPIMGVAFWDESVEIKAETVSVTFEEGVPVALNGKRIDDAVELIMEANRIGGRHGLGM
                     SDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEK
                     VEDAPFTPADRIGQLTMRNLDIVDTRGKLAVYTDAGLLAANNSVIPQLGKK"
     gene            6973..>7139
                     /locus_tag="PWY43_001939"
     CDS             6973..>7139
                     /locus_tag="PWY43_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL2136797.1"
                     /translation="MKKLLVIRNDKIGDFMVCFPAFAMLKQSMPNVEITALVPSYTAP
                     LAELCPSIDKI"
BASE COUNT         2162 a         1520 c         1364 g         2093 t
ORIGIN      
        1 tgtgaatgat aaatggcata agcgacactc tcttcgttga aacaccataa atcctctaat
       61 gaaccgccac ctcgcccgac aattaataca tcacactcat tgcggcggtt tgccagttca
      121 atcatattca caatatcttg cgttgcttct ttgccttgca cgagagccgg gtaaataacc
      181 actgataagc tcggatctcg tcgttgcaaa atatgtaaaa tatcttgtaa tgcggctccg
      241 cttgaggagg taataatacc aacggtttta acaaaagtag gaattggctt tttatgctct
      301 tgagcaaaca agccttgagc tgaaagtttt gctttaagtt gctcaaattg ctgctgtaac
      361 aagccttcgc ccgccggttg catactctca ataatgagct gatagtcacc tcgtggctca
      421 tagagtgtaa cacttgcccg caccagcacc tgcataccgt tttgtgggcg aaaattcact
      481 cttacatttt tcattttaaa catcgcacaa cgcacttgtg ctttgtcatc ttttaaagtg
      541 agataccaat gccccgaaac tggttggctg aaatttgaga tttcgccggt tagccaaata
      601 tggctcattt ccatttcgag caaatggcgg acggaatagt tgagttgggt aacagttaaa
      661 atattgctca taataatcaa aaaagcgtaa atgtagtaga agtgtaacga gaaatgaaag
      721 cgagaggaag cttttgcagt caacaagcgg ttattttttg ctgttttttg gcaaataaaa
      781 cgaaaaccct cgatgtgatt cactatcgag ggtttggaat gtggttgcgg gggccggatt
      841 tgaaccagcg accttcgggt tatgagcccg acgagctacc aagctgctcc accccgcgtc
      901 tgatgaggct ataatatatc aatttgacat aatgacaagt aaaatcatac aaaattgctc
      961 taagtgatgt aaattacaac agatgtagta taaaaatacg cttattgttg taaaaacaat
     1021 caaattatat tctggtttta ttttgtcgct gtttttgcta ttcttctgtt tatttttcac
     1081 attttattaa cgatataagg atattcgatg aattggaaag catataaaaa agctattgcg
     1141 gcagctagcg tggtagcgat tgcaacggta ttagtggcgt gttcgtcttc aggccaaaaa
     1201 ggcaaaacaa cctcttatga tgcggaaaaa gctaaatttg gagcaaaata taatggtcgt
     1261 cactttatgc cttatggcac acattttcaa gctggacgtt taggattaaa tgctgctcct
     1321 gttaatcaac tagacttttt acagcagctt tctaatgtca gagcctatag tagcttggca
     1381 gattcttatg ctatcgctta cggaaaaatc agtcgttgga tctcatcggg cggtaatatt
     1441 aatgatttag caaaatatgg tattagtact cgtttaatgc gtggcgaaga tggctaccag
     1501 aatgtgttaa tgacagggta ttactcgccg gttatcaaag cccgtagaac gcctcagggg
     1561 caataccaac acccgattta tgcgatgccg tctagcaaac gtttttcacg ggcggaaatt
     1621 tacgcaggag cattgcaagg tcagggctta gaactggctt atagtaattc aatgctcgat
     1681 aacttcttat taggtgttca aggtagcggt tacgttgact ttggtgatgg tcgcttaaac
     1741 tattttgctt atgcagggca aaatggcttt aaatatgcca gcgttgggcg tttattagta
     1801 gaagatggcg aaattccgaa agagaaaatg tcagtgcagg ctatccgtga atgggggcaa
     1861 cgtaacccta gccgtgttca aggtttgttg gagcgtaatc catcgtatgt tttctttaaa
     1921 aacgatccta ccggtcaggt aaaaggctct gccggtgtgc cgttagtacc acttgcatct
     1981 gttgcatcag atcgtagcat tgtgccatca ggtagcgtgt tgttggtcga aatgccgttg
     2041 attgatagca aaggaaattg gaccggcaaa cacgaaatgc gtttaatggt ggctcttgat
     2101 gttggcggtg cagtgaaagg ccatcacttc gatttatatc aagggattgg cgataaagca
     2161 ggccatatcg caggtttaat gaaacattac ggccgtgttt gggtattaaa ttaatacagg
     2221 gtaactatgt taaaaaaact atctgccata ttcttacttc taccgagtac ttctaccgag
     2281 cgccgtgctt gcaaataatt tgcaaaatat aaccgcttca actgagccta aatataaaat
     2341 ctcagaaata gatgtcagaa ttttgatacg ccagctcaat aatattgagc aatgtatcta
     2401 tcctgagtta gctaaacccg gttaccagca aatctacgca aattggaatc tggctgaaaa
     2461 tttaacgatg caatattttg aatatcagct tctaaaagag ttgctcggag aggaaaatca
     2521 gaaactgatg caaaatgata cgctttctac cgagtatttc catttactgc attctcgttt
     2581 aaatcaccaa aaagcaaatg tagatcccga aaaatgcgat acatttaaac ctagatataa
     2641 agagatttat aaaagtatgg aaaatgcact cactaagaaa aatcaatagt aaaaaactcg
     2701 cagttttcac tgcgagtttc ttttatcttt cgttatgaat ttctaaattg gtggcttgtt
     2761 ttgccgcctc tgcttttgct ttatcgtttc gagatcttgc tatactgtct aaatagcatt
     2821 tatctacatc acctgtaata tattctcctg taaatacaga gtcatcaaag cgatgaatag
     2881 tcggattttc cagttgaata gacttataaa gtgcttcaag gtcttggaaa atcaatttat
     2941 ccacccctat catctgtgca acctcttcca ctgagcgatc ataagccact aattcttcac
     3001 aagtcggcat atcaatgcca tacacattcg ggtagcgaat ttccggtgcg gcagaggcaa
     3061 aataaacccg ttttgcacca gctgctcgtg ccatttccac gatttgttca gacgttgtac
     3121 ctcgtacaat agaatcatca accagtaaca cgcttttgcc tttaaattca gaggcaatcg
     3181 catttaattt acggcgaacc gagcttttac gctgtgcttg ccccggcata ataaaagttc
     3241 gagctacata gcggttttta acaaaccctt gacgataggg tttatacaac atattagcaa
     3301 tacgtaccgc aatatcattt gaggtttcag gaatcgggat caccacatca atatcatcga
     3361 taattcgtcc ccattcacgt ttaattttct cacctaataa ttcgcccata tgcactcgtg
     3421 cagaatatac agaaacgcca tcaatgacgg aatcaggacg ggcaaaataa acatattcaa
     3481 aaatacaagg attcagtttt ggattatcgg cacaaatttg cgaatgtaat tgcccatcaa
     3541 aggtaacata aatcgcttca cccggcagca catctcgcac aaattcaaac cccactacat
     3601 caagagccac actttccgaa gcaaacatat attcggtttt accctcgatt tcacgtttac
     3661 ccagcactaa cgggcgaata ccgaatgggt cacggaaagc aaccataccg tgtccgataa
     3721 tcatcgcaat gcaagcataa gcaccacgaa tgctatcatt ggttttacga accgtttcaa
     3781 agatattgtc tgggcttaaa tgctgagtgg aatagagatc taaaaagtaa gcaaaaatat
     3841 taagaaggga ttcagaatca gaattagtgt tcacatggcg gcgggcttcg ttgtataagc
     3901 gagctttaag ttccgcatta ttagttaaat taccgttgtg aactaaggta ataccgaaag
     3961 gtgaattgac ataaaatggc tgggcttcag acacacttga gctacctgct gttgggtagc
     4021 gaacgtgacc aattccaaca ttgccttgta atctcaccat atgctcttgc tggaaaacat
     4081 cgctgactaa gccgttagct ttgcgtaagc ggaaacgatt ttcatcgtct atggtgacga
     4141 tacctgcggc atcttgtcct cggtgttgaa gtaatgttaa accatcataa atcgcctgat
     4201 tcaccggcga attcccaata ataccgacaa tgccgcacat atagaaaaac ctctttattt
     4261 agtcgtgttt aaaaaactgg aatttgcttg taactgttca aagaaccact tcacaataaa
     4321 gtcaaaatgc ggaattaata gcgatgattt ccataattcc gtttggcttg cttgggtaaa
     4381 ggtatctaag aaaaataaaa tagccgacac aattaaaata ccacgcaatg ccccaaaagc
     4441 accgcctaaa atacggtcgg tgatagacat tccgctcaaa tcaatcactt tgcctaataa
     4501 accgttgatc acaccacaaa caattaaagt caaaatgaac agaatcgccg ctgcaatacc
     4561 attgcgtaaa aattctgaat tttgcaaata aaccgaatta atttgtgtga gataaccgct
     4621 taaataagga taaaacgagc ttgccacaaa aaaggcgatt acccaaccaa gtagcgataa
     4681 tgcttcattg gtaaaacctc gccataaact gataagcact gaaaaagcaa tgattccgat
     4741 aacaattaaa tcaaccataa cacaccttaa agcgtaaagc tctcctattc tacaaaaaaa
     4801 gaaaacgttt gcgtagcact atttcgctaa aaataagtgc tttatgcaaa tacaagcggt
     4861 taaattcggt aaaaaattta cacaatccga ccgcttgtaa aacctagtct gcaatataac
     4921 caacatcgtc caacgttgga ttatttgctc cttgtttagc cagctcatcg caaatctcat
     4981 tttcacgatg tcctgaatgc cctttcaccc aactccattc aatttggtgg cgttgaattt
     5041 cttggtctaa agcaacccat aaatctttat ttttaactgg ctttttatcg ctggttttcc
     5101 agtcattttt cttccagtta aaaatccatt tttggatccc gtttttcata tattggctgt
     5161 cactattgag ttgcacctta caaggctctt ttagcattgc taaagcctca atcaccgccc
     5221 gcaattccat tcggttattg gtggtttgaa aatagccttt gctcacggtt ttttcgtacc
     5281 cgttataacg cagcaaaata ccaatccccc ctttgccggg gttgcctaaa caagaaccgt
     5341 ctgtaaaaat ttcaactaat ttcatcatca cgctttgaaa ataaaagccc caaagcatta
     5401 actttggggc agcttttagg ctaaattcta aaagttatca gaatggagat caactgatta
     5461 tttttttcca agttgtggaa tcacgctatt attagccgcc aataaacctg catcggtata
     5521 aactgctaat ttaccacggg tatcgacaat atctaagtta cgcattgtta attgaccgat
     5581 acgatctgcc ggcgtaaacg gtgcatcttc tactttttcc atacttaaac gttctgcttc
     5641 gtaggttaag tttggcgatt cagtgttaag aatagtgaaa tcgttaccac ggcgtaactc
     5701 aagcgtgacc gtaccggtaa tcgcttttgc cacccaacgt tgagccgttt cacgcaacat
     5761 taaagcttgc gggtcaaacc agcggccttg atacaataaa cgccctaaac gtaatccgtt
     5821 aatgcgatat tgctcaatcg tatcttcgtt gtgaataccg gtaactaaac gctcgtaagc
     5881 gatgtgtaat aatgccattc ccggtgcttc ataaatacca cgagatttcg cttcgataat
     5941 acggttttcg atttggtctg acattcccaa accatgtctg ccaccaatgc ggttagcctc
     6001 cataattaat tcaacggcat catcaatacg tttaccgttt aacgccaccg gcacaccttc
     6061 ttcaaaggta acagaaacag tttccgcttt aatttcaact gattcgtccc agaatgccac
     6121 gcccataatc ggttttacga tcttcatacc ggtgcttaat tcttctaaat ctttcgcttc
     6181 gtgggttgca cctaacatat tcgagtctgt agagtaggct ttttccaccg acattttata
     6241 gtcaaaaccg ttttcgatta agaatttaga catttccaag cgaccgccta attcatcaat
     6301 gaatagttgg tctaaccacg gcttataaat ttttaatttt ggattggtta ataaaccgta
     6361 acgatagaaa cgctcaatat cgttaccttt aaaggttgaa ccatcgcccc agatattcac
     6421 atcatcttct ttcattgccg caaccaacat tgtgccggtt accgcacgac caagtggcgt
     6481 ggtgttgaaa tacggaatac cgccggttga aatatggaaa gcaccgcatt gaattgcggc
     6541 aatgccttcg tgagctaact gttggcgaca gtcgattaat cgtgcatttt cagccccata
     6601 ttccatcgct tttttcggaa ttgcgttgta atcatcttca tccggctgac ctaaatttgc
     6661 ggtgtacgca taaggcactg cccctttttt acgcatccat aacagtgctg ctgaggtatc
     6721 taaaccgcct gaaaaggcga tacccacttt ctgccctaaa ggtaaagctt ctaaaattgt
     6781 tgctgacata cgaattccta taaattttga aagtaaaaat attcaaacgc aaacgcttac
     6841 gttcaagcaa aatattctag ctgaaaagta gtaaaaattc atctaaaatt tagcccgatg
     6901 atttgcaaat tttttgtgaa atttgaccgc ttataattta ctgaaaacct aaaagcaata
     6961 gagaaaatca gaatgaaaaa actactcgta atccgtaatg ataaaattgg ggactttatg
     7021 gtctgctttc cggcatttgc tatgttgaag caatcaatgc cgaatgtgga aattaccgct
     7081 cttgtgccaa gttatactgc tccacttgcc gagctttgcc cttctattga taagattat
//
LOCUS       DANKBV010000115         7138 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.115, whole genome shotgun sequence.
ACCESSION   DANKBV010000115 DANKBV010000000
VERSION     DANKBV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.115"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..257)
                     /locus_tag="PWY43_001940"
     CDS             complement(<1..257)
                     /locus_tag="PWY43_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2136798.1"
                     /translation="MSFYNDLMESLTAMKEHLEGKRTLRTETLSRPEPLQISADEVKA
                     IRAKLNLSQAVFAKRLRTSVKTYQGWEQGKSTPNPQATILLR"
     gene            complement(424..987)
                     /locus_tag="PWY43_001941"
     CDS             complement(424..987)
                     /locus_tag="PWY43_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2136799.1"
                     /translation="MNAVFMTTNNTPILFTENSGGTERRRVIFKFDRIVPIEERDFNL
                     IDKIQAETGGIIRLLFDTFPEPLEAKALLEKQRVSQEALAVKMEVNHVLEFAQEFEIL
                     PTVNGLAMGSSLNNGTRDSAIYPAYIYFCQLNDIEPINRRTFTRAFKQALKELDKGEY
                     QTRESNGRTVTNVHYIDKLRTFNKWQG"
     gene            complement(1243..1656)
                     /locus_tag="PWY43_001942"
     CDS             complement(1243..1656)
                     /locus_tag="PWY43_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136800.1"
                     /translation="MKHELNLAGITALMSEPIKATLSFLDFPHIRIERLYHDNGRPFF
                     IGVLSLDLEDHINIEMAGATGFLEALAITAYHQQLTATPLLVNDDLLSEINKLPHPQR
                     TNPFIDRHGNLERAKTILAGLFMVLTNADKWQLFI"
     gene            complement(1663..1914)
                     /locus_tag="PWY43_001943"
     CDS             complement(1663..1914)
                     /locus_tag="PWY43_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136801.1"
                     /translation="MRLAIRHYLDNDKGNQTPLFTFLSLYSETEPYPLPELLIVLGNR
                     IAKLEQQHNAMPSETDSITLGILRKQLSQLLKVAERIKE"
     gene            complement(2007..2618)
                     /locus_tag="PWY43_001944"
     CDS             complement(2007..2618)
                     /locus_tag="PWY43_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL2136802.1"
                     /translation="MMKEINQAVFPKVFHKETVAMTNTLKVAEYFEKRHDNILKRLET
                     LECSAEFSLLNFKAAKYIDEQGKSRKMYLMTQDGFTLLVMGFTGKKAIQFKESYITAF
                     NAMRKQIIARAELKSDQLRLNEAIKLNLEKTGKQDIHAYARENNLVYITALGATAKKW
                     LLAQGYSANDEIRHHLNEQQLKLVDDLLAENAVMIKLGLEYDQ"
     gene            complement(2626..2859)
                     /locus_tag="PWY43_001945"
     CDS             complement(2626..2859)
                     /locus_tag="PWY43_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDL2136803.1"
                     /translation="MTESTITHLADDVRIIRPIHARQKLGVGKTTFQDWQNPKSKRYR
                     PDFPKKIQLGANSVGYLESEINAFIQSLADNRV"
     gene            complement(3048..4043)
                     /locus_tag="PWY43_001946"
     CDS             complement(3048..4043)
                     /locus_tag="PWY43_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136804.1"
                     /translation="MLTQEFLSLSEASDFVKSKGLDLEECDLIFLASKKEFRVFNNIY
                     SIPRLNDSNPLYKVNGLKGVFELLLETQLLDVAERLTKALADSNIEVSEYSNGYHTVL
                     VLKNKQTKNEVEVLYAPYGEFQISELRYSYASVYFPVFDGLGKQFDIINGMVESRMLE
                     SLSERNFLNSKLFYKMLQKSLNNYYVVNSPFDQFYFKLKEIIFYPIGLRPYCVSSEEL
                     DNNNDLKEIYDEEDFSNIFYFQKEELEAYIDKNLKIETKLEQTDITSIFDGDIPPKTD
                     QDKLAHFIELIIQKSEFMKDGKIPTYSELHTMLSHKYPHDAIPSKNTIKKYLGQH"
     gene            complement(4440..4838)
                     /locus_tag="PWY43_001947"
     CDS             complement(4440..4838)
                     /locus_tag="PWY43_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2136805.1"
                     /translation="MKPYQTIKDTNGNDLFVLVPVAEFEQLTGDNLYDFPYDDETDDD
                     LVEVEYDKDEFDGVTIPFEVVKIKLEQNINNLGAWRIYRNLSQQEVAEKTGLSQSAIS
                     QAERKGNRPQKRTREKLAKIYGCTPEQLAG"
     gene            complement(4881..5147)
                     /locus_tag="PWY43_001948"
     CDS             complement(4881..5147)
                     /locus_tag="PWY43_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2136806.1"
                     /translation="MNKITFTGKADKQLSKIDSRYILAIIEAIESLALFPNVEKDIKR
                     LQGYISRFRMRVGRYRVIFDWLEGEPRIIEIQTIAKRDERTYTH"
     gene            complement(5520..6110)
                     /locus_tag="PWY43_001949"
     CDS             complement(5520..6110)
                     /locus_tag="PWY43_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2136807.1"
                     /translation="MPEFCERLQNSNLELLTRYLIQWQLLTMIRANEAVTAEYTDIDE
                     EKRLWVIPPQKMKQKHGGKPHIVPLSKQAYSLLQKIKTLANGNGYLFPSVRTKTGRMN
                     PQTVNKAIRKSLGYSKEALTAHGLRKTTSSYLHEKGILPDVVELCLSHTIGGLRGVYN
                     KAKYLPQRREAMQLWGDYVAQCMAKSGVSHLKLVAW"
     gene            complement(6205..6771)
                     /locus_tag="PWY43_001950"
     CDS             complement(6205..6771)
                     /locus_tag="PWY43_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDL2136808.1"
                     /translation="MARSTTPLNSTQIDKAKPKEKEYTLADGKGLYLLVKPNGTKLWR
                     FNYYKPYTNPKKQALISIGSYPDVSLQQARQTKDEYNALLAQNIDPQAHRIETTITEQ
                     NRLVNTFAYVAEQWKASKEDVIKPLTLSKNWRIAEIYLLPHLKDLPIDKIIPAVVKPV
                     LDIPYKANKAKMFRKSMRLLNEILDYRK"
     gene            complement(6973..7058)
                     /locus_tag="PWY43_001951"
     tRNA            complement(6973..7058)
                     /locus_tag="PWY43_001951"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7022..7024),aa:Leu,seq:gag)
BASE COUNT         1859 a         1374 c         1387 g         2518 t
ORIGIN      
        1 cgcaaaagaa tagttgcttg tgggttcggt gtgcttttcc cttgttccca cccttgatag
       61 gttttaacgc tggttcgtaa acgcttggca aataccgctt gagagagatt tagctttgct
      121 ctgattgctt ttacttcatc ggctgaaatc tgtagcggtt cagggcgtga tagggtttca
      181 gttcgcagtg ttcgcttgcc ctctaaatgc tctttcattg cggttaggct ttccattaaa
      241 tcattataaa aactcatatc taccccttag agagcagttt caataaaagt taaattcatc
      301 ttatctattc cgttgcggtt ctactgggtg ttatagtatc cgcaaagtga ggataaatca
      361 ataaaacaag taacaaaaaa gcctagcata tttcatctag gctttgcttg ttggcggtag
      421 gggttagcct tgccacttgt taaaggtgcg tagtttgtcg atatagtgaa catttgtaac
      481 cgtccgcccg ttgctttccc ttgtttggta ttcccctttg tctaattcct taagggcttg
      541 tttaaacgct cgggtaaagg ttcggcggtt gattggctct atatcgttga gctggcaaaa
      601 gtagatataa gctggataaa tcgcactgtc tcttgtgccg ttattaagtg aacttcccat
      661 tgccagcccg tttactgtcg gcaatatttc aaactcttga gcaaactcta aaacgtggtt
      721 cacttccatt ttaaccgcta gagcttcttg gctcactcgt tgcttttcta gtagggcttt
      781 tgcttcgagt ggttcgggga aagtgtcaaa cagtaggcga ataatgccgc ccgtttcggc
      841 ttgaatttta tcaattaaat taaagtccct ttcttcaatc ggcacaatgc gatcaaactt
      901 aaaaatcact ctccgccttt ccgtgcctcc gctgttttcg gtaaacagaa tcggggtgtt
      961 attggttgtc ataaagaccg cattcacttt ttggctaaac ggctttttgt gttttgggtt
     1021 gaagaatata tcatcgcccc ctgtgattgc cctaatcgtt gccccgtccc cggtatattt
     1081 cggttgctct ccggagatga taaatgtttt atcaatgata atgcaacgct taacttcatc
     1141 atcaagagct tttagcgtta tgcctacgtg gttattttca cctcccagca gtttcgctat
     1201 ttcggtgtaa gtgcttttcc ctgttccgcc tagccctgtt acctagataa ataattgcca
     1261 tttgtcggcg ttagttagca ccataaacaa gccggctaaa atcgtcttag ctcgctctaa
     1321 gttgccgtgt cggtcaataa atgggtttgt tcgttgtggg tggggtagct tgttgatttc
     1381 actcaataag tcatcattca ctaatagcgg cgtggcggtt agctgttgat gataggcagt
     1441 aatcgctaga gcctctaaaa agcccgtagc ccctgccatt tcaatattga tatgatcttc
     1501 aaggtcaaga gaaagcacac cgataaaaaa cggtctgccg ttatcgtggt acagtcgttc
     1561 aattcggata tgcggaaaat ctaaaaagct caaggttgct ttgataggtt cactcattaa
     1621 agcagtaata cccgctaaat taagctcgtg tttcattttg gtttattcct ttattctttc
     1681 cgccactttt aacagttggc taagttgttt tcttaaaatg ccgagtgtga ttgaatcggt
     1741 ttcgcttggc attgcgttat gttgttgttc cagcttcgct attcggttgc ctagcacaat
     1801 taaaagctcg ggcaatgggt aaggctcggt ttcagaatag aggcttaaaa acgtgaataa
     1861 gggtgtttga ttgcctttgt cgttatcgag atagtgccga atagccagcc gcattaaaac
     1921 ggggttttgc ttggcggtaa tgcacctcat tatttaccct cttgtttgcg gtagtaactt
     1981 aatgcggttt cttgtagcct tgcttttcat tggtcgtatt ccagccctaa cttaatcatc
     2041 acggcatttt ctgccagcaa gtcatcaacc agtttgagct gttgttcgtt taaatggtgt
     2101 cggatttcat cattcgctga ataaccttga gcaagtagcc attttttagc agttgctcct
     2161 agtgccgtga tgtaaacaag gttattttct cgggcgtagg cgtgaatatc ctgcttgcct
     2221 gttttctcaa gattgagttt gattgcctcg tttagtcgga gctgatcgct tttcaattca
     2281 gcccttgcga taatttgctt tctcattgca ttaaacgctg tgatatagct ttctttgaat
     2341 tgtattgctt tcttaccggt aaagcccatc actaaaagcg taaagccgtc ttgggtcatt
     2401 aaatacatct ttcttgattt gccttgctca tcgatatatt tagccgcctt aaaattgagg
     2461 aggctaaatt cagcagaaca ttctaaggtt tcaaggcgtt tcaaaatgtt gtcgtgtcgt
     2521 ttctcgaaat attccgccac tttgagcgtg ttagtcattg ctacggtttc tttgtgaaag
     2581 actttcggga atacggcttg attgatttct ttcatcattt cccccttata ctcggttatc
     2641 tgccagcgat tgaataaagg cgttgatttc gctttcgaga taacccacgg aatttgcccc
     2701 tagctgaatc ttcttcggga agtctgggcg atagcgtttt gatttcgggt tctgccagtc
     2761 ttgaaaagtg gttttgccta cgcctaactt ttggcgtgcg tgaatagggc ggataatgcg
     2821 aacatcatca gctaagtgtg tgatagtgct ttcggtcatt tatattcccc tatgctgtta
     2881 aattgaaatg cttaaacctt gaaagggttt aggcggttgc agtagggatt ttaagaaatg
     2941 aaaagttatt caggggaaga gtgaactatt gaacagtgtt cattgaacaa atgaactgtt
     3001 gttgaacggt ttaaataaaa aaatccgcca taattagcgg attttgatta gtgttgtcct
     3061 aaatatttct tgattgtatt tttagatggg attgcatcgt gtggatattt atgacttagc
     3121 attgtatgaa gttcactata ggttggtatt ttaccgtctt tcataaactc acttttttga
     3181 ataattagct cgataaaatg agccaattta tcttgatctg tttttggtgg gatatctcca
     3241 tcaaatattg atgttatgtc tgtttgttct agttttgttt ctatttttag gtttttatca
     3301 atgtaggctt ctagttcttc tttttgaaaa tagaatatat ttgaaaaatc ctcttcatcg
     3361 taaatttctt ttaaatcatt attattatct agttcctcag atgatacgca ataaggccgt
     3421 aagccaatag ggtaaaatat gatttctttt aacttgaaat aaaactgatc aaatggactg
     3481 tttaccacat aataattatt taaacttttt tggagcattt tataaaatag ttttgaattt
     3541 aaaaaatttc tctcggatag agattctaac attcggcttt caaccatacc attaataata
     3601 tcaaattgct ttcctaaccc gtcgaatact ggaaaataaa cagacgcata agaataacgt
     3661 aattcactaa tctgaaactc gccataagga gcatataaaa cttcgacttc attctttgtt
     3721 tgtttatttt taagtaccaa taccgtgtga tagccgttcg aatactctga aacctcaata
     3781 ttgctatctg ctagggcttt tgtaagacgt tcagccacat ctaataattg tgtttctaga
     3841 agtaactcaa aaactccttt taatccattg actttataaa gaggattgct gtcatttaat
     3901 ctaggaattg aatatatatt attgaatact ctaaattctt tttttgaagc tagaaaaatt
     3961 aaatcacatt cttctagatc taatccttta gatttaacaa aatcactagc ttccgaaagt
     4021 gataaaaatt cttgagtaag catacataac gcccctttaa cgctgccctt tactgtgaga
     4081 gcgtggaaag agaaataaag ggctttctcc tttcgttttg gtagctactc cgccactatc
     4141 cacactaatt tgaataaatc cgtatagtta cgaattgaac tttataggct gtattttgcc
     4201 aattattcag ataaattaca aataataaac tgtgttttat tacagtgatt taaaataggc
     4261 taacggtagg tttctgaacg ctataaaaaa gggcattcaa gcggtcattt ttcattaaga
     4321 ttttgcgaaa acttgccccg tattgatccg caatcggtgc gtaattggta cagaattaag
     4381 caataaaaaa gccgttaata tcaatcaacg gctatttgaa tgtgaggttt tgacaggtcc
     4441 taacctgcta actgttcagg tgtgcaaccg taaattttag ctaatttttc ccttgtacgt
     4501 ttttgtgggc ggttgccttt gcgttctgct tgtgaaatag cggactgtga aagccctgtt
     4561 ttttctgcca cttcttgttg tgataaattg cggtaaatcc gccacgctcc taagttgttt
     4621 atattctgtt ccagcttgat tttaaccacc tcaaaaggaa tagttacacc atcaaattca
     4681 tctttgtcat attccacttc aactaaatca tcatcggttt catcatcata ggggaaatca
     4741 tataaattgt cccctgttag ctgttcaaat tccgccaccg gcacaagtac aaataaatcg
     4801 ttgccgttag tgtctttaat ggtttgatat ggtttcatct cgtttctccg tgctgtttgg
     4861 ggcatttctg ccccttgtct ttagtgtgta tatgttctct cgtctcgttt ggcgatagtc
     4921 tgaatttcaa taattcttgg ctcgccctcc agccagtcaa aaatcactct ataccgcccc
     4981 acacgcattc taaacctgct gatataacct tgtagccttt taatgtcttt ttctacgtta
     5041 ggaaataatg ctaggctttc aatcgcttca ataattgcca gtatatatcg gctatcaatc
     5101 tttgaaagct gtttatctgc tttccctgtg aatgtaattt tattcattta tgatttcctt
     5161 ataaattcac aataacacca ttatactttt ttatatttca ctaatcaata aattattagc
     5221 tttttataag tataaatcag gttttaaaat aaaatataag taaatagagc agaataaaag
     5281 gcaagtaagc tacttttacc cacttttaac ccactttacc cactgcttac ccactgttta
     5341 cccactgtca ttttttcttt atttttcagt tagttataaa aaaataatag tgaaaagtgg
     5401 gtaaagtggg taaaaatcga aaagtttaca gaattttcat caaggggaat tgctggcgat
     5461 aaaaaagccg tcaggcgata acctaacggc attacaagcg gtcatttttg gcggaaaatt
     5521 taccaggcga ctagttttaa gtgacttact ccgctttttg ccatacattg agcaacataa
     5581 tctccccata actgcatagc ttcccgcctt tgtggtaaat atttagcctt gttataaacg
     5641 ccccgtaatc cgcctatagt gtgagataaa cacagctcta ccacgtcagg caatatgcct
     5701 ttttcgtgta gataactgct tgtagttttg cgtaagccgt gagcggtcag tgcttctttg
     5761 ctatatccta aagactttct gatcgcttta ttaacggttt gcgggttcat tctgcctgtt
     5821 ttcgttctta cacttgggaa taaatagccg ttgccattgg caagcgtttt gattttctgt
     5881 agtagcgaat aagcctgttt tgataatggt actatatgcg gcttgcctcc gtgtttctgt
     5941 ttcatttttt ggggtgggat aacccaaagc ctcttttctt cgtcaatatc tgtatattca
     6001 gctgtaactg cttcatttgc tctgatcata gtaagaagtt gccattgtat taagtatcga
     6061 gtaagtaact ctaaattaga gttttgtaac cgttcgcaaa attcgggcaa ttcctcaaaa
     6121 ggaatataag caaattcccc tcttggtttt gcttcaaatg cttttttgat attgctacaa
     6181 ggatttagcg gaatgatgaa taagctattt acggtagtct aaaatttcat taagtaacct
     6241 catcgacttt ctgaacattt tagctttatt tgctttgtag ggaatatcta aaacaggctt
     6301 cactactgcc ggtataattt tatctatcgg caagtctttt aagtggggga gtaagtatat
     6361 ttcagcaatc cgccagttct tagagagtgt tagcggtttt ataacgtcct ctttgcttgc
     6421 cttccattgc tccgccacat aagcgaaggt gtttactaaa cggttttgtt ctgtgatggt
     6481 ggtttctata cggtgggctt gcggatcgat attttgagca agaagagcat tatattcatc
     6541 tttagtttgc cttgcttgct gtaaagaaac atcggggtaa ctgcctatgc tgattaaagc
     6601 ctgtttttta gggttagtgt atggtttgta ataattaaac cgccacaact tagtcccgtt
     6661 gggtttgaca agcagataaa gccctttgcc gtcggctagg gtatattctt tttcttttgg
     6721 cttggcttta tcaatttgag tgctatttaa tggtgtagtt gaacgtgcca tcttttcccc
     6781 ttgcgttagt tatacgctat taagttttag ttatacgact ttttagattt gctataccgt
     6841 ataactaaac gtataactaa aatcttcgga tttgattgta ctcgataggt atgcatagga
     6901 caataaaaaa gccttgaagt attgattttc aaggctttta aggtacctat agaactctat
     6961 aggtctttaa tttggtgctc tgggcgagac ttgaactcgc acggactgtg tccaccaccc
     7021 cctcaagatg gcgtgtctac caattccacc accagagctt tattcttttt aatgttaagc
     7081 gattattgag ggatatcgct attttctatt tttacaggtt ctacttgctt ttgttcaa
//
LOCUS       DANKBV010000116         7127 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.116, whole genome shotgun sequence.
ACCESSION   DANKBV010000116 DANKBV010000000
VERSION     DANKBV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.116"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            355..2493
                     /locus_tag="PWY43_001952"
                     /pseudo
     CDS             355..2493
                     /locus_tag="PWY43_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694042.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            2564..4252
                     /locus_tag="PWY43_001953"
     CDS             2564..4252
                     /locus_tag="PWY43_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL2136809.1"
                     /translation="MKFYPSLIASAVSFAVVLSGQAAANVPDAGSLQRELDQRQAQQK
                     VKPSGEWFNQAAKAPYTATDGQELTFPLTQVQVFDWTNQEISTPELAPILANYVGKAV
                     SLTDLSNLANEITEFYRSHNYLVAKAILPPQEIEQGKVKILLFKGNVGEVRLQNQSAL
                     SDKFVARLANTTVNGSEFILKDELEKFALTMNDVPGVNTGLELSAGKQAGEANLLIKL
                     QNAERFSGYVSADNQGNKNTGRYRLATGIKANNLIGWGDELKVDLMSSNNANLKNARL
                     DYSSLIDGYATRLGLTANYLDYELGGNFKALQSQGHTHSIGAYVLHPTIRLPDFRLNT
                     KVSFNHQGLTDKQQAVNVAQKRQVNSLTVGINGSWNSLKDGTSYFSLSALFGNLANHT
                     NEQAHYVATDFQPKSRFTVYQYSLSHEQALPKSFAFNLGVNGQFADKTLDSSQKMLLG
                     GLYGVRGHQAGVASVDEGHLVQTELKHYLPLFSKSLLTSSVFYDYGWGKYYKHSGFLA
                     RGVNNSVKLQSIGAGLSLSEAGSYAINASVAKPLGKKIENADKHQFWFSAIKTF"
     gene            4264..7086
                     /locus_tag="PWY43_001954"
     CDS             4264..7086
                     /locus_tag="PWY43_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HDL2136810.1"
                     /translation="MFKRNAISLCILTACSGLAYANTADLPQGERVRVGTATIERTTD
                     KMTVNQSSNKVQIDWNSFDIGKNKEVEFKQPTEMAVAYNRVTGGNASQIQGKLTANGK
                     VYLANPNGVLITKDAEVNVGGLLVTTKDLEKISENGTTKDKFSRKLAKEGKILNQGKI
                     TAKGERAFVVLVGDDVANEGEINAENYYKKETKRVRECYNDPWSWGQQCYDIDKEIVK
                     STSGEVHLSSGENVTFTLSDRYIDVALDDNTVNSIIKNEGAIIANGDITFTAKGRNQA
                     LDSLIINNGVLQANKVQNKNGTIILSAGETQLHEKSQIKGEKVSFAEASYYDGPKENI
                     KVVSKTGSKVTAPVIDFKGKTVNIQGSFGREDRTEHYNDELKTLRTVVNIEVPNTEDI
                     RIADTDNGGSGSFIQAGALGSLLANNGRVNVQGKGFDVSGKLNVNAFKQTDSVLKFIN
                     QADINIHNADIHSNGRLFMLTNLPNDTDFQSNIRITDSKINLGNGAMGFGRSAKRWEM
                     HDVVRRADTELRKKFNLDMKNVELNQVDDFVVAGGFQQVNLDNVKARGKTNFYLDSGN
                     SRVFVGEQIYEYGVTDIEKRTQLANLDQRRKRWNSDNYDEIDLNWEYNQRFDGFAAKH
                     NHRAPIAGTEINIANSDIGLDNGFVHLMAEKINLDDSKINITFNQDNSLDESTQINRL
                     GLNGKVSMKNSHINVVGDEKDGISPARHYATMFLVGELLGEKSSIFLKSHQGAVLRTD
                     SNVKIAGKNSKDDLKITAITTGKKMGNEIIGPDGLPTSEDNDANASNTAFAIGYEPNA
                     TAIVENASITALAPNGGLAYTGTSKTQIDAKDNATFTFFEKPRAGYDKLLGDIKGNPD
                     KLTPRDFARLYDNIKGIKASSLSEQQLGLADTAKKTSEHTLANSLDVENLVSVTVCDE
                     QNKCEERMLGNKNTNAKVIVGDIKFE"
BASE COUNT         2243 a         1522 c         1645 g         1717 t
ORIGIN      
        1 agagctaata ccgctaaaac gcttgcacta attttggtta acgttggaaa tttcatatta
       61 ttctccgctt aatcttagga ttaacttacg aagattacct tttccacaat gaggagtaaa
      121 gaacaaaaag cattttgtta taacgaaatg atataaaaaa taaagtacgg atgcttattt
      181 ttcaaaattt cagataagaa aggaaagata gtagctaatt cttaagataa ccctatttta
      241 aacgtgtata taaaaccttc atatacaaat aattctcatt agctaattga tagcaattct
      301 caatagtgtt atattattta ttgctctacc ttatacctaa aaagaggagt aaaaatgcaa
      361 ctttctaaac tatcccttgc gattactgtt agtttattct ctgcaaatat ttttgcaaat
      421 caatccatag aattagatac cgtcaacgtc attgccaccc gtgatccaag taaatttgct
      481 gatacgccac aaaaacaaac taaagatgca cttttggtaa aacaagcgac gagtgtggcg
      541 gtggcattaa aagcgttgcc gaatgtggat gtgcaaggag gggcccgtgc gattgcgtag
      601 aagccgtcta ttcgtgggtt gagcgataat cgtgttgtgc aggtgattga cggagtgcgt
      661 cagaattttg atttggctca ccgtggttct tattttgtgc cgatgtcatt gattcaagaa
      721 attgaagtaa tcaaaggccc aagcagttcg ttgtggggga gcggtgcctt aggtggtgtg
      781 gtggcaatgc gcacaccgaa tgcgttggat ttattgaaaa ataatgacaa attcggggca
      841 aaaatccgtc aaggttatca aacggcgaat aatctttccg aaaccgaagc ctcggtgttt
      901 gcagcaaatg atcggtttga tgccttgctg agtggtttct acaataatgc tgacaatttg
      961 cgtagtggtg aaggcaggga gctgaataac accggctata agcaagccgg tggcttggta
     1021 aaattcggtt ggcaactgaa tgaggcaaac cgtgtggagc tttcacaccg tgagagccaa
     1081 tttaagcaaa cagcaccaag taacaatgat gtcgaaaatc aatttaccac tgcagacatc
     1141 aacgccaaaa ttgcggcgtg gcacgcagcc aatccgcctt caacaccgaa tttctttgcg
     1201 aaattagcgg cattttatgg cggtttgggc gcacaatttg gtagcgtgag ttatttgagc
     1261 gatcaaaaaa ttaacgataa aagcaccgca cttaactact atttcaaccc gagcaatcct
     1321 tatgtcaatg cgcaagtgac agtctatcaa aacagcacca cggaaaaaga gcaacgtaaa
     1381 gtgagtggtg tggaagatcg aaccaaatta accacgcgcg gcatcaatct gcgtaaccat
     1441 tccgaattgc cttggttctc gttggtctat ggtgtggatt atatgcagga taaagccgac
     1501 accgagcgtg gcacaaacaa tagtgatgcc cgctatcgtt ctaatccgta tcaagcaaaa
     1561 tccaatacga caggtgcata tttaatcgcc catattccgc tttggggcga aaaactgctg
     1621 ttctcgccga gtgtacgtta tgaccgtttc aacacctcaa gcgaggcggt gaaataccaa
     1681 gacagccatt ggtcgccggc ggcaaaattg acatggaaag cgacaacttg gttagatttt
     1741 accgcaaaat ataatgaagc cttccgtgcg ccttcaatgc aagagcgttt tacaggcggt
     1801 tcgcactttg gtactcaagg acagggcggt gcaccgatta tcaatatttt tgtacaaaac
     1861 ccgaatttac gtcctgaaac tgcgaaaaat aaagagatca ctgcccatgt gcattttgat
     1921 gatctgatca cacaagggga taaattcagc gttgaagcca cttatttccg taacgatgtg
     1981 agggatttca tcaatcttca actttacaaa gccaatccgg caagcccgat ggaagtgatt
     2041 ccaacccgtt cgcaatatcg caatgtaacc aacgcacgat taagcggtgt ggagttacaa
     2101 acccaatatc aaaccgagcg tgttacgttc ttggcaaact acggctcaac caaaggtaaa
     2161 gataaagaca gcggagaagc cttgtcgaac attgctgcaa gcaaaatcgg ctttggggtc
     2221 aattatgccg tggtgcaaga caaattcacg gtgggggcaa acgtaacccg ttatcaagca
     2281 caacatcgtg tgccgaaaaa ccacggtgtg acttaccaag gctatacgct gaccgatcta
     2341 cacgccactt acgccccatt aaaaggcgaa tggaaaaacc tgcgtttaga cttggcggtt
     2401 gaaaacctat tcgataaaaa ataccaaccg gcattcagct taatggaagg cagcggtcgt
     2461 aatgtgaaac tcagtgcggc ttacagcttc taatgtcttt aacggaagac ggcacaagcg
     2521 gtcgttttcc ggttattttt tgcaatatta aaagggagtt actatgaaat tttatccgtc
     2581 gctgatcgca agtgcggtca gttttgccgt tgttttgagt ggacaagcgg cagcgaatgt
     2641 gccggatgcc ggctcgttac agcgtgaatt agaccaacgt caagctcaac aaaaagtcaa
     2701 accgagtggc gaatggttta atcaagcggc aaaagcacct tataccgcaa cggacggtca
     2761 agaactgaca tttccattaa ctcaggtaca agtttttgat tggaccaacc aagaaatctc
     2821 taccccggaa ttagccccga ttttggcaaa ctatgtgggt aaagcggttt cgctgaccga
     2881 tttaagcaac ttggcaaatg agatcaccga gttttatcgc agccacaatt atctggtggc
     2941 aaaagccatt ttaccgccac aggaaattga acaaggtaag gtgaaaatcc tgttgtttaa
     3001 aggcaatgtc ggtgaagtgc ggttacaaaa tcagagtgct ttaagcgata aatttgttgc
     3061 acgccttgcc aacaccacag tgaatggttc ggaatttatc ctaaaagacg agctggaaaa
     3121 atttgccctc acgatgaatg atgttcccgg ggtgaatacc ggcttggagc tttctgccgg
     3181 taagcaagca ggcgaggcga atttgctgat taagctccaa aatgccgagc gtttctcagg
     3241 ctatgtatcg gcagataacc aaggcaataa aaataccgga cgttaccgtt tagccacagg
     3301 gataaaagcc aataatctga taggttgggg cgatgagttg aaagtggatt tgatgagttc
     3361 caacaacgct aatttaaaaa atgcgcgttt ggattattcg agcttgattg acggctacgc
     3421 tacccgtttg ggcttaacgg cgaattatct cgactacgaa ttaggcggta actttaaagc
     3481 cctacaatcc caagggcata ctcacagcat cggggcttat gtgctgcacc cgacaatccg
     3541 cttgccggat tttcggttaa acaccaaagt cagctttaat catcaaggtt taaccgacaa
     3601 acaacaagcg gtcaatgtgg cacaaaaacg ccaagtaaac agcttaacgg tgggcattaa
     3661 cgggtcgtgg aattcgctta aagacggcac aagctatttc tctctgtctg ccttgttcgg
     3721 caatttagct aaccacacca acgagcaagc gcattatgtg gcaacagatt ttcagcccaa
     3781 atcccgtttt acggtctatc aatacagcct gtctcacgaa caagcgctgc caaaatcctt
     3841 tgccttcaac cttggcgtga acgggcagtt tgcggataaa accctcgaca gctcacaaaa
     3901 aatgttactg ggcggtttat acggcgtgcg tggtcatcaa gccggtgtcg cttcggtgga
     3961 tgaggggcat ttagtgcaaa ccgaactcaa gcattattta ccgctctttt ccaaaagcct
     4021 gctgaccagc tcggtcttct atgattatgg ttggggaaaa tactataaac acagcggatt
     4081 tttggcgcgt ggtgtaaaca atagcgtgaa attacagagt atcggagcag ggctttcgct
     4141 ctctgaagca ggcagttatg ccattaacgc cagtgttgcc aaaccgctgg gtaaaaaaat
     4201 cgagaatgca gataaacacc aattctggtt ctccgccatc aaaacctttt aaggagacaa
     4261 tttatgttta aacgtaatgc aatttctctc tgcattttga ccgcttgcag cggtttagcc
     4321 tatgccaata cagcagattt gccgcaaggt gaaagggtgc gtgtcgggac ggcaacgatt
     4381 gagcgcacaa ccgataaaat gaccgtgaat caatccagca ataaagtaca aattgattgg
     4441 aacagctttg atattggtaa gaataaagaa gtggaattca aacagccgac agagatggcg
     4501 gtggcatata accgtgttac cggcggaaac gccagccaaa ttcaaggcaa gctgaccgca
     4561 aacggcaaag tgtatttagc caacccgaac ggtgtactca tcaccaaaga tgcggaagtg
     4621 aatgtgggcg gtttgttagt gaccacaaaa gatttagaaa aaatcagcga aaacggcaca
     4681 acaaaagata aattcagccg caaattagcg aaagaaggca aaattcttaa ccaaggtaaa
     4741 attactgcca aaggcgagcg ggcttttgtg gtattggtgg gcgacgatgt ggctaatgaa
     4801 ggggaaatta acgccgaaaa ttactacaaa aaagagacca aacgggtgag agaatgctat
     4861 aacgacccgt ggagctgggg gcaacaatgc tacgatattg ataaagagat cgtaaaaagc
     4921 accagtggcg aagtgcattt atcctccgga gaaaatgtca cctttacctt aagcgaccgc
     4981 tatattgatg tggcgttaga cgataatacg gtcaatagca ttatcaaaaa tgaaggtgcc
     5041 attattgcca acggcgacat tacttttacc gccaaagggc gaaatcaagc cttggacagc
     5101 ttaattatca ataatggcgt gttacaagcc aataaggtgc aaaataaaaa cggcaccatc
     5161 attttatctg ccggtgaaac ccaattacac gaaaaaagcc agattaaagg tgagaaagtg
     5221 agctttgccg aagcttccta ttacgacggc ccgaaagaaa acatcaaagt ggtgtccaaa
     5281 acaggctcaa aagtgaccgc acctgtgatc gatttcaaag gcaaaaccgt gaatattcaa
     5341 ggcagttttg gtcgtgaaga cagaacagag cattacaatg atgaactcaa gacattgcgt
     5401 acagtggtaa atattgaagt gccgaatacg gaagatattc gcattgccga taccgacaac
     5461 ggcggtagcg gttcctttat acaagcgggt gctttaggct cattacttgc aaataacggt
     5521 cgagtgaatg tacagggcaa aggctttgat gtctcaggca agttgaatgt gaatgccttt
     5581 aaacaaacgg attccgtgct gaaattcatc aatcaggcgg atatcaatat ccacaacgca
     5641 gatattcact caaacgggcg tttatttatg ctgactaacc tcccgaatga cacggatttc
     5701 caatcaaata tccgcattac cgacagcaaa atcaacctag gaaacggtgc aatgggcttt
     5761 ggtcgcagtg caaaacgttg ggaaatgcac gatgtggttc gacgtgccga tacggaactg
     5821 cgtaaaaaat tcaatctgga tatgaaaaac gtggaattaa accaagtgga tgattttgtg
     5881 gtggcgggcg gtttccaaca agttaatttg gataacgtga aagcccgtgg caaaaccaac
     5941 ttctatctgg attccggcaa ctcccgcgtg tttgtcggtg agcagattta tgaatatggc
     6001 gtaaccgata ttgaaaaacg cacccaattg gcgaatttag accagcgtag aaaacgttgg
     6061 aattcggata actatgatga gatcgatctc aactgggaat acaaccaacg ttttgacggc
     6121 tttgcagcaa aacacaatca ccgcgcgccg attgccggta cggaaatcaa tattgccaat
     6181 agtgatatcg gcttagataa cggttttgtg catttaatgg cagaaaaaat caatctggac
     6241 gacagcaaaa tcaatatcac cttcaaccaa gacaacagct tggatgaaag tacccaaatc
     6301 aaccgcttag gactaaacgg caaagtgtcg atgaaaaaca gccatattaa cgtagtgggc
     6361 gatgaaaaag acggtatctc accggctcgc cattatgcca cgatgttctt agtgggggag
     6421 ctgcttggtg aaaagagttc tattttcctc aaatcccacc aaggtgcagt attgagaacc
     6481 gacagcaatg ttaaaattgc cggtaaaaac agcaaagacg acctcaaaat taccgccatt
     6541 accaccggta aaaaaatggg caatgaaatc atcggaccgg acggcttgcc aacctcggaa
     6601 gataacgatg ccaacgccag caacaccgcc tttgccatcg gctatgaacc taacgctacg
     6661 gctatcgtcg aaaatgcaag cattaccgca ttagcaccaa acggcggctt ggcatacacc
     6721 ggcacgtcta aaacccaaat tgacgcgaag gataatgcta cctttacctt ctttgaaaaa
     6781 cctcgagccg gctatgacaa attgcttggc gacatcaaag gtaaccccga taaattaacc
     6841 ccacgcgatt tcgcccgttt atacgacaac attaaaggca tcaaagccag cagcctatcc
     6901 gaacaacagc ttggcctagc agacaccgcg aagaaaacca gcgaacacac cttagcaaac
     6961 agcctagacg tagaaaattt ggtttcagtg acagtgtgtg atgagcagaa taagtgtgag
     7021 gagcgtatgt taggtaacaa aaataccaat gctaaagtta ttgtcggaga tatcaaattt
     7081 gagtagcgtt gaagaaagtg gtagaagctc agacttgatc tgataaa
//
LOCUS       DANKBV010000117         7094 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.117, whole genome shotgun sequence.
ACCESSION   DANKBV010000117 DANKBV010000000
VERSION     DANKBV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.117"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(83..955)
                     /gene="rsmI"
                     /locus_tag="PWY43_001955"
     CDS             complement(83..955)
                     /gene="rsmI"
                     /locus_tag="PWY43_001955"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL2136811.1"
                     /translation="MKNRQNSSEINPNYGILYIVATPIGNLGDITQRALDTLSSVDLV
                     AAEDTRHSGLLLSHYGIKKPFFALHDHNEQQKAVVLVEKLAKGENIALISDAGTPLIS
                     DPGFHLVRHCRQAGVKVVPIPGACAAVTALCASGIASDRFCFEGFLPAKTKSRCDKLT
                     ELENEPRTLIFYESTHRILDTLADMQTVFGEDRYVVMAREITKTWETIYGDQLGNLIE
                     WLNEDSNRIKGEIVLVVEGKPQSSEEAFSSQAIKLLTLLCQELPLKKAAAIVAETFGY
                     KKNALYQYGLEHFE"
     gene            1029..2750
                     /locus_tag="PWY43_001956"
     CDS             1029..2750
                     /locus_tag="PWY43_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HDL2136812.1"
                     /translation="MATILNHTMKKALVPTAIALFISACTSVNPVTESIKNEAYSSSE
                     FYINKADQTKEAEDKISYQLLAVRKLIDENKEYEAQNTFSEILTAEMNEVQKLEYALV
                     SAQLAALQGKNEQATSQLKAIQDPLLSSAQRLRYIQTQARIAANQKDVIGIVRARSQL
                     NNFYKMNRERQENNDIIWQTLRDANRGMLEKTVPEAGEMELAGWLALINIYNQNVTTP
                     AQMPQAINNWKAQYPSHSAVAVMPTELQGVSNFQQTQLNSVALLLPLSGDAKILGDII
                     KRGFNDAKADDSTAVQTFDTDSSDVNSLISQAKQQGANVIVGPLLKSRVDEMLLSPEI
                     QNVNILALNATENVRNIAQVCYYGLSPESEAQSGAEKIYQDGNSVAIVAAPQDDYGNR
                     SAEAFAKRWRQLTNSDADVRYYNQPLDAVAAIQNAGVSKAALYILGNAEQVLEIKQGI
                     DSSTLKDRLAIYTSSRSNSPNNGIDFYTSMEGVKFSEVPLLADQSSSEYKKAENLANS
                     DFSMMRLYAMGADAWSLATKFNEFRHIPGYKISGLTGELKAGQHCNIERSLSWMQYRN
                     GAIQQAY"
     gene            2725..3096
                     /locus_tag="PWY43_001957"
     CDS             2725..3096
                     /locus_tag="PWY43_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDL2136813.1"
                     /translation="MELFNKLINKREQGSFYEQKARVFLEQQGLRFVAANQAFKGGEL
                     DLIMQDGQTVVFVEVRQRKSNRFGSAVESIDYRKQQKWQNAANMWLLKQNQSLETANC
                     RFDVVAFEGNQTPVWIPNFLG"
     gene            3102..3686
                     /locus_tag="PWY43_001958"
     CDS             3102..3686
                     /locus_tag="PWY43_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2136814.1"
                     /translation="MLNKIQDRFTESIQTQISAAELLPAKLSEAANRIVACLLRGDKV
                     IVCGHGRSYGNAELLVSHLLHRYELARPSFAAVQLQLSGVLAGVVAQDNELEAVYKKQ
                     LQAVAKEGDLFIVFSPVGNEECILQAIHTAKNQHLDLDIIALTGSNNDHTLGLLDESD
                     IEISAPSTNQLRVIESHHFYVNLLCELIDHLLFS"
     gene            3696..4286
                     /gene="yraP"
                     /locus_tag="PWY43_001959"
     CDS             3696..4286
                     /gene="yraP"
                     /locus_tag="PWY43_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDL2136815.1"
                     /translation="MNKLANKLLKGSLLVLGFISLQGCITTAVVTSVAVATKVATDPR
                     TTGTQVDDEILEEKVAYNINKDEQIKQEARINVVAYNGKVLLIGQAPSMDVVENAKNL
                     AAGAEGVTEIYNEIRQGEKIGFGQITQDSWITTQVKSKLLVNGEVKATEVKVVTENGE
                     VFLMGKVSQNQADAAAEAARNVGGVTKVVKVFSYAQ"
     gene            4576..4767
                     /locus_tag="PWY43_001960"
     CDS             4576..4767
                     /locus_tag="PWY43_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136816.1"
                     /translation="MLFKEQGYDEFLAEQIKAGQEAIARGETFTKEQINNELNHLFAD
                     MQKRQQLRENENLFEVNYA"
     gene            4760..5047
                     /locus_tag="PWY43_001961"
     CDS             4760..5047
                     /locus_tag="PWY43_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2136817.1"
                     /translation="MPKSWELSLTARSNVIEILQSVEDYTGHWLSVVSLWEQLQNKLD
                     LIAFMPTIGRRREDGTLETFCRNYRIVYEIHLEKVYIITIIHSRRLYPRPS"
     gene            complement(5194..6336)
                     /gene="argE"
                     /locus_tag="PWY43_001962"
     CDS             complement(5194..6336)
                     /gene="argE"
                     /locus_tag="PWY43_001962"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDL2136818.1"
                     /translation="MKQISFIQRYAQLIELPTISSLVAEEDLSNRRLIELLATWLADF
                     GFKTEILAVEGSHNKYNLLATYGEGEGGLLLAGHTDTVPFDEGKWRFNPFQLTEKAGK
                     LYGLGTADMKGFFAFVVEVVSQLDLTQIKKPIRILATADEETTMLGARTFAQHSHIRP
                     DCAIIGEPTSLKPIRAHKGHIGESVRITGRSGHSSDPAKGINAIELMHQSIGYLMKMR
                     DELREKYHNPLFQVTHPTMNFGNIHGGDAINRICACCELQFDMRPLPNMAVQDLYAMV
                     NEHLKPMLEQYGDLIEIRHLHDGIPGYECEHSAQIVQVVEKLLGEKCDAVNYCTEAPF
                     IQQLCPTLVLGPGSIEQAHQPDEFLEMKYIEPTKELLTKLIYHFCG"
     gene            complement(6340..>7094)
                     /locus_tag="PWY43_001963"
     CDS             complement(6340..>7094)
                     /locus_tag="PWY43_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2136819.1"
                     /translation="TTLSKAIENYFTSGSFGVKFYPFLMHPCFRKSCAFLIFIIGMVS
                     SFSGLFYLVAEHITILFSERSLTTYFHHSTLELMIPVGTMIDEKISELSPLSVVMRSM
                     FVLQAVVFFFIYAIGITHIAHNKLRVIGLLLSLCFAIGCSLVAGIQPSSRGEFGIYNL
                     GASITFLIGNLTLIIAGFDLYHPSMQFFKRFSIGAGVIGAICILITIFLPTLFSPLIE
                     RAGIYTIMLWEILAGFAIIKRLLKLPSFRQQG"
BASE COUNT         2163 a         1399 c         1574 g         1958 t
ORIGIN      
        1 gaaatgtttc gttcagaaac atttttcaga gagaggggag tgccaatctg caaaaaactc
       61 acaaaatctg accgcttgta tgttactcaa aatgctccaa tccatattga tacagggcat
      121 ttttcttata gccgaaggtt tctgccacaa tggcagcggc ttttttaagc gggagttctt
      181 ggcagagtaa tgtcagtaat ttgatcgctt ggctggaaaa ggcttcctca ctactttgcg
      241 gtttaccttc aaccaccaga acaatttcgc ctttaattcg gttgctatcc tcatttagcc
      301 attcaattaa gttacccagt tgatctccat aaatggtttc ccacgttttc gtgatttcac
      361 gcgccattac cacatagcga tcttcgccaa aaacagtctg catatcggca agagtgtcta
      421 aaatacggtg ggtggattca taaaaaatca gtgtgcgagg ctcgttttca agctcggtaa
      481 gtttatcgca acgggatttt gtttttgccg gtaagaaccc ttcaaaacaa aaacggtctg
      541 aagctatgcc tgaagcacat aaggcagtaa cagcggcaca agccccgggg atcggcacaa
      601 cttttacccc ggcttggcgg caatgacgga caagatgaaa gcccgggtcg ctaatcagtg
      661 gcgttccggc atcggaaatt aacgcaatat tttccccttt tgctaatttt tcgactaaaa
      721 cgaccgcttt ctgctgttcg ttatggtcgt gcaaggcaaa aaacggtttt ttaataccgt
      781 aatggctcag caataaaccg ctgtggcggg tgtcttcggc ggcgactaaa tcgacagagg
      841 aaagggtatc taacgctcgt tgggtaatat cgcctaaatt gccgatgggg gtggcaacaa
      901 tatataaaat tccgtaattt gggttgattt cagatgaatt ttgtctgttt ttcatttgct
      961 tttagctcgt ttgataagta agatttcaca ttaaatttag cacattatat tctattgagg
     1021 atctgacaat ggcaactatt ttaaaccata caatgaaaaa agcattggtg ccaacggcga
     1081 ttgcattatt catttctgcc tgtacatcgg taaatcctgt caccgaatcc attaaaaatg
     1141 aagcctattc aagctcggaa ttctatatta ataaagcaga tcaaaccaaa gaagcggaag
     1201 ataaaatttc gtatcaactt ttagctgtac gcaaattaat tgatgaaaat aaggaatatg
     1261 aggctcaaaa tacctttagt gaaattctga ccgcagagat gaacgaggtg caaaagttag
     1321 agtatgcctt ggtttccgcc cagcttgccg ccttacaagg gaaaaacgag caagcaacaa
     1381 gccaattaaa agccattcaa gatccgctat taagttcggc acagcgtttg cgttatattc
     1441 aaacccaagc ccgtattgct gccaatcaga aagatgtgat tggcattgtg cgtgcaagat
     1501 cgcaacttaa caatttctat aaaatgaacc gtgaacgcca agaaaataac gatattattt
     1561 ggcaaacctt gcgtgatgca aatcgaggaa tgctggaaaa aactgtgcct gaagcgggtg
     1621 agatggagct tgctggttgg ttggcattaa ttaatatcta taatcaaaat gtgacgacac
     1681 cggcacaaat gccacaagca attaataatt ggaaagccca atacccgagc catagtgcgg
     1741 ttgccgtaat gccaacggaa ttacaagggg tgagtaattt ccaacaaacc cagttaaaca
     1801 gtgtcgcatt gctattgcct ctaagtggtg atgcaaaaat tttgggtgat attatcaagc
     1861 gtggttttaa tgatgctaag gctgatgact caaccgcagt gcaaactttt gataccgatt
     1921 catctgatgt taattcctta atttcacagg caaaacagca aggggcaaat gtgattgtcg
     1981 ggccattgct caaatctcgt gttgatgaaa tgctacttag cccagaaatc caaaacgtga
     2041 atattttggc actgaatgcc actgaaaatg tgcgtaatat tgctcaggtg tgttactacg
     2101 gtttatctcc tgaatctgag gcacaatcag gggcggaaaa aatttatcaa gacggtaata
     2161 gtgtcgctat cgttgcggcc cctcaagatg actacggcaa ccgctcggca gaagcctttg
     2221 ccaaacgctg gcgtcagctt accaatagcg atgctgatgt gcgttactac aatcaaccgc
     2281 ttgatgccgt tgccgctatt caaaatgcag gtgtgagcaa agcagcttta tatattttgg
     2341 ggaatgccga gcaagtatta gaaattaaac aaggtattga tagctcaacc ttaaaagatc
     2401 gtttagcgat ctatacctca tctcgcagta attcgccaaa taacggcatt gatttttaca
     2461 cttcaatgga aggggtaaaa tttagtgaag tgccactgtt agctgatcaa agttctagtg
     2521 aatataaaaa agccgagaat ttagccaata gcgatttctc aatgatgcgt ttatacgcaa
     2581 tgggggctga tgcgtggtca ttggcaacca aattcaatga attccgccat attccgggct
     2641 acaaaatttc aggtttaacc ggtgagttaa aagccggaca acattgtaat attgaacgta
     2701 gtttatcttg gatgcaatat cgcaatggag ctattcaaca agcttattaa taaacgggaa
     2761 caaggctcgt tttatgaaca aaaagcccga gtgtttttag aacagcaagg gcttcgtttt
     2821 gttgccgcta accaagcatt taaaggtggc gaactggatc tgattatgca agacggacaa
     2881 accgttgtgt ttgtagaagt tcgccagcgt aaaagcaacc gcttcggttc agccgttgag
     2941 agtattgatt accgcaagca acaaaaatgg caaaatgccg ctaatatgtg gttgcttaaa
     3001 caaaaccaaa gtttagaaac tgcaaattgc cgttttgatg tggttgcttt tgaaggaaac
     3061 caaacgcctg tgtggattcc taacttttta ggttagaaag aatgttaaat aaaatccaag
     3121 atagatttac cgaaagtatt caaacccaaa tttccgccgc tgagctattg ccggctaaat
     3181 taagtgaagc ggcaaaccgc attgttgcct gtttgttgcg tggcgataaa gttatcgtct
     3241 gcggacacgg tcgctcttac ggtaatgccg aattattggt cagccactta cttcaccgtt
     3301 atgaactggc aagaccgagt tttgctgccg tacaactgca attaagcggt gtattggcag
     3361 gagttgtggc acaagataac gagttagagg ctgtttataa aaaacagttg caggcggttg
     3421 caaaagaggg tgatctcttc attgtttttt cgcctgtggg taatgaagaa tgtatattgc
     3481 aagctattca tacggcgaaa aatcaacatt tagatttaga tattattgct ttaacaggca
     3541 gtaataacga ccacacactc ggcttattag atgaaagtga tattgaaatt tcagcccctt
     3601 ccaccaatca actgagggtg attgaaagcc atcattttta tgtgaattta ttatgtgaac
     3661 ttatcgacca tttactattt tcgtaaggag agattatgaa caaattagca aataaactat
     3721 taaagggcag cctattagta ttaggtttta tctctttaca aggttgtatc accacggcgg
     3781 tggtgacttc tgtggcggtt gcaactaaag tggcgacaga cccacgcacc accggcacgc
     3841 aagttgatga tgaaatttta gaagaaaaag tcgcttacaa cattaataaa gacgagcaaa
     3901 tcaaacaaga agcccgaatt aacgtggtgg cttacaacgg caaagtgtta ttaatcgggc
     3961 aagcaccaag tatggacgta gtggaaaatg cgaaaaacct cgctgctggg gcggagggcg
     4021 tgacggaaat ttacaacgaa atccgccaag gcgaaaaaat cggctttgga cagattacac
     4081 aagacagctg gattaccacc caagtgaaat ccaaactgtt agtaaatggc gaggtaaaag
     4141 ccaccgaagt gaaagtcgtc accgaaaacg gcgaagtgtt tttaatgggc aaagtcagcc
     4201 aaaaccaagc tgatgccgcc gccgaagccg ctcgcaatgt aggtggagta accaaagtgg
     4261 tgaaagtatt tagttatgcg cagtaattga taagctcaat tagtttatat ttttgtaact
     4321 cacaagcggt ctaaatttgt catttttttg caaatttaga ccgcttgttt gatataagaa
     4381 gatgacctta gataggaaga taaaatctat gagtggatga tcgtaatgat gatttttaat
     4441 tcatttgact cgaaaaatat gcacacccta tcaacataag gattcccttg actcttcatt
     4501 gctttgagcg tagatttaaa cttaaactat gtcttgaaca tagtggaatc aagacacaaa
     4561 tcaaaggagc agaatatgct attcaaagaa caaggatatg atgagtttct tgcagagcaa
     4621 attaaagctg gtcaagaagc aatagctcga ggagaaacct ttaccaaaga acaaatcaat
     4681 aacgaattaa atcatttatt tgcggatatg cagaaacgtc agcaattacg agaaaatgaa
     4741 aatttgtttg aggttaatta tgcctaaatc ttgggagctg tcacttaccg caaggtctaa
     4801 tgtcattgag attttacagt cggtagaaga ttatacaggg cattggttga gcgtagtttc
     4861 actttgggaa caattacaaa ataaactcga tctcattgca tttatgccta caatagggcg
     4921 acgtagagaa gatggcacat tagaaacatt ctgccgcaat tatcgtattg tctatgagat
     4981 acacctagaa aaagtatata ttattactat aattcattct cgccgtttgt atccaagacc
     5041 tagctagaga ttaataccta tctaacagaa tctccttaaa tcaatgatga agtatggaag
     5101 tttataggtc actcaatact aaatatcaag cggtcatttt tgccgattct tttgcaaaaa
     5161 gtatggcgaa aatgagcgct tgtttctgtt gatttatcca caaaaatgat aaatcaattt
     5221 agtcaataac tcttttgtcg gttcgatata tttcatttcc aaaaactcat cgggctggtg
     5281 ggcttgttca atggagccgg ggcctagtac cagcgttggg cagagttgtt gaatgaatgg
     5341 ggcttcagtg cagtaattga cggcatcgca tttttcgccc agtagctttt caaccacttg
     5401 cacgatttgt gccgagtgtt cgcattcgta gcccggaatt ccatcgtgta aatggcggat
     5461 ttcaattaaa tcgccatatt gttccagcat cggttttaaa tgctcgttta ccattgcata
     5521 caaatcttgc accgccatat tcggtagtgg tcgcatatca aattgcaatt cgcaacaggc
     5581 acaaatgcgg ttaatggcat cgccgccgtg aatattgccg aagttcattg tcgggtgggt
     5641 gacttggaac agcggattgt ggtatttctc tcgcagttca tcacgcattt tcattaaata
     5701 accgatagat tggtgcatta attcaatcgc attaatgcct ttggcggggt cgctggagtg
     5761 tccgcttctg ccggtaatgc gaaccgattc gccaatatgc cctttgtggg ctcgaatcgg
     5821 tttgagcgag gtcggctcgc caataatcgc acaatcgggg cgaatgtggc tgtgctgagc
     5881 aaaggttctt gcccccagca ttgtggtttc ttcatcagcg gtggcaagaa tgcgaatcgg
     5941 ctttttgatt tgggttaggt cgagttggct cacaacctcc accacaaagg cgaaaaagcc
     6001 tttcatatct gccgtgccta agccgtagag tttgcctgct ttttcggtta attggaatgg
     6061 attgaagcgc cattttcctt catcaaacgg cacggtgtcg gtatgccctg ccaataataa
     6121 accgccctca ccctcgccat aggtcgcaag taaattgtat ttattatggc taccttctac
     6181 cgctaaaatc tcggttttaa agccgaaatc tgccagccaa gtggcgagca attcaatcaa
     6241 acggcggttc gataaatctt cttcggcaac aaggctggaa atggtcggta gctcaatcag
     6301 ttgagcatag cgttgaataa aggaaatctg tttcattggt tatccttgtt gtctgaatga
     6361 gggcaatttc aacaggcgtt taatgatggc aaaaccggct aaaatttccc acagcataat
     6421 ggtataaata cctgctcgtt caattaacgg gctaaacagg gttggtagga aaatcgtgat
     6481 taaaatacag attgccccga taacgcccgc accaatgcta aagcgtttaa aaaattgcat
     6541 tgaagggtgg tagagatcga agcctgcgat gatcagcgtt aaattgccga ttaaaaacgt
     6601 aatgcttgcc cctaaattgt aaatgccgaa ttcgccacga ctgcttggct gaataccggc
     6661 aactagtgag cagccaatcg caaagcatag cgaaagcaat aagccgatga cccgcaattt
     6721 gttgtgagca atgtgggtga tgccgattgc ataaataaag aaaaaaacga ctgcttgtag
     6781 cacaaacatt gaacgcatta ccaccgaaag tggtgataat tcgctgattt tttcatctat
     6841 cattgtgccg actggaatca tcaattcaag ggtgctatgg tggaagtaag tggtgagcga
     6901 tctttcgcta aataaaatcg tgatgtgttc cgccaccaaa taaaatagcc cgctaaaact
     6961 tgaaaccata ccaataataa aaatcaaaaa ggcacagctt ttacgaaaac aagggtgcat
     7021 tagaaaaggg tagaatttca ccccaaatga accgctggta aagtagtttt cgatggcttt
     7081 ggaaagcgtg gtta
//
LOCUS       DANKBV010000118         7082 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.118, whole genome shotgun sequence.
ACCESSION   DANKBV010000118 DANKBV010000000
VERSION     DANKBV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.118"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..2879
                     /gene="mukB"
                     /locus_tag="PWY43_001964"
     CDS             <1..2879
                     /gene="mukB"
                     /locus_tag="PWY43_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDL2136820.1"
                     /translation="QAQQAVSLRQKLNDLEQRLQQQQNAQRLVAEFNQKSQQQLNSAE
                     ELDAYFEEQQARLEDLEAELSDFVEQRSTQRQQREQLNQQYQQLAKNAPAWHTAQSAL
                     TRLEEQCGEKFEASQSVMQFMQNMLSKEREATLARDELARKEQLLDEQINRLSQPDGS
                     EDARLNQLAERFGGVLLSELYDDVSIEDAPYFSALYGEARHAIVVRDLEAVKTQLEKL
                     DDCPSDLYLIEGDPSAFDDAVFSAEELGEGVVVKVSDRQWRYSKFPEVPLFGRAAREK
                     HLATLKAERDETAEKHAERAFDVQKCQRLHQHLSQFIGTHLNLAFQENPEMLMQEIAA
                     ERAEIERELAQSAGNEQQLRHQLESVKAQLQMLNKILPLANLLADETLEARAEECREQ
                     LELAEEDEMFIRQFGATLSQLEPIAAALKSDPALFEQLEADYRRSVAEQKVLQQKVFS
                     LADVMNRRLHFSYQETVGTEGSALTEQLRQRLENAQREREQARDQLRQAQAQLSQYNQ
                     VLTGLRSSYEAKNQMLQELIREIDDLGVRGDSGAEERARFRRDELQQRLSQQRSRKGY
                     LDKQIAVIEAEMDNLNRTLRKAERDYHTQRELVVQAKASWCIVQRLSRNSDVEKRLNR
                     RELAYQSAEELRSISDKALGALRTAVADNEYLRDSLRASEDSRKPENKVAFFIAVYQH
                     LRERIRQDIIKTDDPIDAIEQMEIELSRLTNELTGREKKLAISAESVANILRKTIQRE
                     QQRILQLNQGLQNIAFGQVKGVRLVVNIRDTHAILLNALSNNREEHKDLFDSQKLSFS
                     EALAMLYKRVNPHIEMGQRTPQTIGEELLDYRNYLDLEVETFRGADGWMRAESSALST
                     GEAIGTGMSILLMVVQSWEEEARRMRAKDILPARLLFLDEAARLDATSINTLFELCER
                     LDMQLLIAAPENISPERGTTYKLVRKITNNQEYVHVVGLKGFAK"
     gene            2921..3301
                     /locus_tag="PWY43_001965"
     CDS             2921..3301
                     /locus_tag="PWY43_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2136821.1"
                     /translation="METLAIFLFSEIWKFDFVFTFIFKILKINVFFSDLINMKLARKA
                     QRRLSFLRVFSLAFAAFIVNTTEFIPIALLSDIASSFQMEPSSAGIMMTVYAWVVFLL
                     SLPLMLLTANMERKRLLLMLFMGE"
     gene            3298..3946
                     /locus_tag="PWY43_001966"
                     /pseudo
     CDS             3298..3946
                     /locus_tag="PWY43_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4013..5746)
                     /locus_tag="PWY43_001967"
     CDS             complement(4013..5746)
                     /locus_tag="PWY43_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HDL2136822.1"
                     /translation="MFARLRSLLPTNSRQYREETSQKITWGHWFALFNIVLALLISSR
                     YAFNADWPNTLVGKLYFFVSLFGHFSFVVFAGFLLILFPLSFIVKNHRTYRGLSVILA
                     TIGQTVLLVDTEVFKQFYLHLSPLVWDLLVNPEEGELTRKWQLLFVPMPLLLLAEMLY
                     SRWCWQKLRSFSRQKWGKYVALFFVISFTATHLIYAWADMNAYRPITAQRANYPLSYP
                     MTARNFLEKHGFIDRVEFNQEIEESGRLDTFYLNYPKSPLIATKTPSTNILLINLSGL
                     SKQIINAETMPKLTEISQQSHRFMQHYSSGDSASAGALGLFYSLSGKYLDSVLADKTV
                     SPLIEIARKSDYQLGLFSHNGFADPIYHQAIFSGILLPTAHSNVGAIGQWKNWISSRS
                     LENPFFSYLDLRMPFAHTAQEQAKLLDWQFAEIWQHLTQNALLANTLVIITADISNEV
                     GAENSFDKQHIQVPMMFYWQGESKEYHFLSSHLDIMPTLLSQFFGVQNPLNDYAQGMD
                     LSTQNQRLWVSSSNHKWNVAIMPNGDQYQIDRKGNFKHFNSNGEHEKDARPPLALFLQ
                     MIQQSNQFIEK"
     gene            complement(5751..5972)
                     /locus_tag="PWY43_001968"
     CDS             complement(5751..5972)
                     /locus_tag="PWY43_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1414 domain-containing protein"
                     /protein_id="HDL2136823.1"
                     /translation="MATKSKYHTKQFEQLLADLIATIEKHKAPVDLSLMALGNMITNI
                     LIENIQSPAQREALADAFADALKQSLKTK"
     gene            6211..6783
                     /locus_tag="PWY43_001969"
     CDS             6211..6783
                     /locus_tag="PWY43_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2136824.1"
                     /translation="MSRRSEPKEEMVNRILSATESLIVQEGLQNLSMRSIAKEAGIAS
                     GTLYLYFKTKDDLLHSLAAQLLECYNGYMHFEFKPEMALFEQYKALVNKKWQFLIERL
                     DLVKQFQAVIGIDELIRETIADETSFWNKLILEGKKQRIIADLPNELLYGLSIGTLVD
                     IQYLQNLNQENQFDGYLDEIILRTWKAITF"
     gene            6814..>7082
                     /locus_tag="PWY43_001970"
     CDS             6814..>7082
                     /locus_tag="PWY43_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HDL2136825.1"
                     /translation="MTTETQKLGKGRKFLIVLTLLIVLIVFAAIVGLQKIGAMKKAEA
                     AANMPEAVSEVTAMQVTTQEWTPTISAVGYIRPNQGAMLSAETSGV"
BASE COUNT         2097 a         1317 c         1631 g         2037 t
ORIGIN      
        1 tgcaagctca gcaagcggtg agtttacgtc aaaaactcaa cgatttagaa cagcgtttgc
       61 aacaacagca aaatgcccag cgtttagtgg cggaatttaa ccaaaaatcg caacaacaac
      121 tcaattcagc ggaagaatta gacgcctatt ttgaagagca gcaagctcgc ctagaagatt
      181 tagaggcaga gctttcggac tttgtagaac aacgttcaac ccaacgccaa caacgtgaac
      241 agcttaatca acaatatcag caattagcga agaatgcacc ggcttggcat acggcacaat
      301 cggctctgac tcgtttagaa gagcaatgtg gcgagaagtt tgaagccagc caatcagtga
      361 tgcagtttat gcaaaatatg ctgagcaaag agcgtgaagc cacccttgct cgtgatgaat
      421 tggctcgtaa agaacaactg cttgatgagc aaattaatcg cttaagccag ccggacggct
      481 cggaagacgc ccgcttaaac cagttggcag aacgttttgg tggagtgtta ctctctgagt
      541 tatatgatga tgtatcaatt gaagatgcgc cttatttctc cgccttatac ggcgaggctc
      601 gccacgctat tgtagtgcgt gatttagagg cggttaaaac acagctagag aaattagacg
      661 attgcccaag tgatctttat ttaattgaag gtgatccgtc cgcctttgac gatgcggtat
      721 ttagtgccga ggaattaggc gaaggcgtgg tggtgaaagt ctccgatcgc caatggcgtt
      781 attctaaatt ccctgaagtg ccgttattcg gacgtgctgc tcgtgaaaaa catttagcga
      841 ctttaaaagc agagcgtgat gaaaccgcag aaaaacacgc tgaacgtgcc tttgatgtgc
      901 agaaatgcca gcgtttacat caacacttaa gccaatttat cggcacgcac ttaaatttag
      961 cgttccaaga aaaccctgaa atgttaatgc aagagattgc ggcagaacgg gcagaaattg
     1021 aacgtgaatt ggcacaatca gccggtaacg aacagcaatt acgtcaccaa ttagaaagcg
     1081 taaaagccca gttgcaaatg ttgaataaaa ttctaccgct tgcgaattta ttagccgatg
     1141 aaacgctgga agcacgagcg gaggaatgtc gtgagcaatt agaattggca gaagaagatg
     1201 aaatgtttat ccgccaattt ggagcaacat taagccaact tgagccgatt gcagccgcat
     1261 taaaaagtga tccggcttta tttgaacagt tggaagccga ttaccgccgt tcagttgccg
     1321 agcaaaaagt gttacagcaa aaagtgttca gcttggctga tgtaatgaat cgccgcctcc
     1381 attttagcta tcaagaaacg gtgggtacag aaggctctgc attaaccgaa cagctacgcc
     1441 aacgtttgga aaatgcacaa cgtgagcgtg aacaggctcg tgatcagctg cgtcaagctc
     1501 aagctcaact ttcacaatat aaccaagtat tgaccggctt acgcagttct tatgaggcga
     1561 aaaaccaaat gctacaagag ttaattcgtg aaattgacga tttgggcgtg cgtggtgata
     1621 gcggagcgga agagcgagcc agattccgcc gtgatgaatt gcaacaacgt ttaagccagc
     1681 aacgttcccg caaaggttat ttagataaac aaattgcggt gatcgaggcg gaaatggata
     1741 accttaaccg caccttacgt aaagccgaac gtgattatca tacacaacgt gaattggtgg
     1801 tgcaggcaaa agccagctgg tgtattgtgc aacgtttatc tcgtaacagc gatgtggaaa
     1861 aacgtttaaa tcgccgtgaa ttggcttatc aatcggctga agaattacgt tcgatttcgg
     1921 ataaagctct cggggcatta cgcaccgccg tggcggataa tgaataccta cgtgatagct
     1981 tgcgggcttc tgaagatagt cgcaagccgg aaaacaaagt ggcgttcttt attgcggttt
     2041 atcagcattt gcgtgagcgg attcgccaag acattatcaa aaccgatgat ccgattgatg
     2101 cgattgagca gatggaaatc gagctttcac gcttaaccaa tgaattaacc ggtcgtgaga
     2161 aaaaactggc gatcagtgcg gaaagtgtgg caaatatttt acgtaaaacc attcagcgtg
     2221 aacaacaacg cattttgcaa ctgaaccaag gtttacaaaa tattgcattc ggtcaggtaa
     2281 aaggggtgcg tttggtggtg aatatccgag atacgcacgc tattttactc aatgcgttat
     2341 ccaataatcg tgaggaacac aaagatttat tcgacagcca aaaactgagc ttctccgaag
     2401 ccttagcgat gttgtataaa cgggtgaacc ctcatattga gatggggcaa cgtacaccac
     2461 agaccattgg tgaagagtta ttggattacc gtaactactt agatttagaa gtggaaacct
     2521 tccgtggtgc agacggctgg atgcgtgcag aaagtagtgc attatcgacc ggtgaggcga
     2581 tcggtacagg tatgtcgatt ctcttaatgg tagttcaaag ctgggaagaa gaagctcgcc
     2641 gtatgcgtgc caaagatatt ctaccggctc gcttattgtt cttagatgaa gcggctcgat
     2701 tagatgctac ctcaattaat acgctatttg aactctgcga acgtttggat atgcagttat
     2761 taattgcagc ccctgaaaat attagccctg aacgaggcac aacttataaa ttggtgcgta
     2821 aaatcaccaa taaccaagag tatgttcacg ttgttggttt aaaaggcttt gctaaataaa
     2881 acttagcact ttaatctaaa tatgtataaa aagattgttg atggaaacat tggcaatctt
     2941 tttattttca gaaatttgga aatttgattt tgtttttaca tttattttta aaattttgaa
     3001 aataaatgtg tttttctcgg atttaattaa tatgaaactt gctcgaaaag ctcaacggag
     3061 attatcgttc cttagagtat ttagtcttgc ctttgcggct tttatagtaa acaccaccga
     3121 atttattcct attgcattgc tttcagacat tgcaagcagt ttccaaatgg agccttcatc
     3181 ggcaggcatt atgatgacgg tttatgcgtg ggtggtattt ttattatcgt taccgttgat
     3241 gttactcacg gcgaatatgg aaagaaaacg gctgttgctg atgttattta tgggcgaatg
     3301 atcgggcaag ccttaggctg gcgggcaacc tttggcatta ttgggattat cgctttgatt
     3361 gtgctaatga tgatttggcg gttgctgcct tatttaccaa gcaaaaatgc aggctcgata
     3421 aaaagtttgc ctttgttatt taaacgcccg atgttaatcg gcatttattt gctggtggca
     3481 ttgatcgttt caggacattt taccgcttac agctatattg agccgtttat gattaaaatc
     3541 agttcggtga gtgtggaaaa caccaccttt attttgctgg tttttggctt ggcaggtgtg
     3601 gtggcaagtt ttttatttgg tcggttatat ggcaaagccc cgaataaatt tatcttaagc
     3661 gggatcgcca ttattatgtg cagccagctt tgtttactgt ttttgagtga ctcaacagtg
     3721 gcaatgttta tggtcgcttt tgtctgggga attggcatta ccggaatgag cattgcgttg
     3781 caaatgaagg tgcttgaact tgcccctgat acgactgatg tcgcaactgc aattttttcc
     3841 ggcacttata atttaggcat tggcggtgga gcattactgg ggagcagctt ttgtagcaat
     3901 ggtttggttt gtatttgtta gcttgaaata tagacagctc agatagtgac aagcggtcat
     3961 attttgctga aattttgcaa attttcctgc aaaaatgacc gcttgttgaa cattactttt
     4021 caataaattg attgctttgt tgaatcattt gtaagaacaa cgctaatggt ggtcgagcat
     4081 ctttctcgtg ttcgccattg ctgttaaagt gcttaaagtt gcctttacga tcaatttgat
     4141 attgatcgcc attcggcata atcgccacat tccatttgtg attagaactg gatacccata
     4201 aacgttggtt ctgggtgctt aaatccatac cttgtgcata atcattcagc ggattttgca
     4261 cgccgaagaa ttggctcagt aaggttggca taatatctaa atggcttgaa aggaaatggt
     4321 actctttgct ttcgccttgc cagtagaaca tcatcggcac ttgaatgtgt tgtttatcga
     4381 agctattttc tgctccaact tcattactaa tatcggcggt aatgattacc agcgtattcg
     4441 ccaatagtgc attttgggtc aaatgttgcc agatttcagc gaattgccaa tctaataatt
     4501 ttgcctgctc ttgagcggtg tgggcgaagg gcattcttaa gtctaaataa ctaaagaacg
     4561 gattttccaa tgagcgagag ctgatccagt ttttccattg cccaatcgcc cctacattac
     4621 tgtgcgccgt aggcaataaa ataccagaga agatcgcctg atgataaatc gggtcggcaa
     4681 aaccattatg tgaaaacagc cctaattgat agtccgattt tctagcgatt tcgataagcg
     4741 gtgacacggt tttatcggct aaaacggaat ccaaatattt accgcttaaa ctgtaaaata
     4801 gacctaatgc accggcagag gctgaatcac cactactgta atgctgcata aagcggtggc
     4861 tttgttggct gatttctgtc agcttaggca ttgtttcggc atttattatc tgtttagata
     4921 aacctgataa attaatcagt aaaatattag ttgagggcgt tttggtcgca atcaagggag
     4981 attttggata attcagatag aaagtatcta gtctgccact ttcttcaatt tcttgattaa
     5041 attctactcg gtcaataaaa ccgtgttttt ctaagaaatt acgagccgtc atcgggtaag
     5101 aaagtgggta gtttgctcgt tgagcggtaa tcgggcggta ggcattcata tctgcccacg
     5161 catagattaa atgagtagcg gtaaagctga tcacgaaaaa gagagcgaca tatttgcccc
     5221 atttttggcg gctaaaacta cgcagtttct gccagcacca gcgagagtag agcatttctg
     5281 ccagcaaaag gagtggcatt ggtacaaaca gcagttgcca tttacgggtc agttcccctt
     5341 cttccggatt aactaacaga tcccatacaa gcggtgataa atgcagataa aattgcttga
     5401 agacttcagt atccaccaat aacacggttt gcccgatggt ggctaaaatc acggaaagcc
     5461 cacgataggt gcgatgattc ttaacaataa aactgagcgg aaacagaatt aatagaaatc
     5521 ctgcaaagac cacaaagctg aaatgcccga acaggctgac aaagaaataa agtttgccta
     5581 caagcgtgtt cggccagtct gcattaaatg cgtaacgaga ggaaatgagt agtgccaaca
     5641 caatattaaa tagtgcaaac caatgccccc acgtgatttt ctgtgaggtt tcttctcgat
     5701 attggcgaga attggtcggc aataaactgc gtaggcgtgc aaacatcatc ttatttggtt
     5761 tttagtgatt gttttaatgc atcggcaaag gcatcagcga gagcttctct ttgtgccggg
     5821 ctttgaatat tttcaattaa aatgttagtt atcatattgc ctaatgccat taacgataaa
     5881 tccaccggtg ctttatgctt ttctatggtg gcgatgaggt cagccaatag ctgctcaaat
     5941 tgtttggtgt ggtatttaga tttcgttgcc atattgaaat aaagatgatc ctataattag
     6001 gtacaaatag tagcacaaaa ataataataa agtagcgtta agcgttattg tttgagtgat
     6061 ttttgcaaag ttttagccaa aaatgaccgc ttgttcaata aatgaggaat tttgcaacta
     6121 ttttatttta atttgatagg tgttttctgc tttctttttt cataaaaaaa gctaaaatga
     6181 acggctgttc attataggta aggagataaa atgtctaggc gaagcgagcc aaaagaagag
     6241 atggtaaacc gtattttatc ggcaaccgag tcgctgattg tgcaagaagg tttgcaaaac
     6301 ctttcaatgc gtagtattgc aaaagaagca gggatcgctt ccggtacatt atatctttac
     6361 tttaaaacca aagatgattt attgcactca ttggctgccc aattactcga atgttataac
     6421 ggttatatgc attttgagtt taagcctgaa atggcgttat ttgagcaata taaagcgttg
     6481 gtcaataaaa aatggcagtt tttaatcgag cgtttagatt tggtaaaaca gtttcaagcg
     6541 gtgataggga ttgatgaatt gattcgggaa accatagctg atgaaacttc cttttggaat
     6601 aaattaattt tagaagggaa aaagcaaagg attattgctg atctacctaa tgagttgctt
     6661 tatggattaa gcatcggcac attggtggat atccaatatc tgcaaaatct gaatcaagaa
     6721 aatcagtttg atggatattt agacgagatt attttacgca cttggaaagc aatcactttt
     6781 taagtttctt tttattagtt ttatggagaa tgtatgacaa cggaaacaca gaagctgggt
     6841 aaaggcagaa agtttttaat tgtactcaca ttgttaatcg tattgattgt gtttgctgca
     6901 atcgtagggt tacaaaaaat cggggcaatg aaaaaagccg aagcagcggc aaatatgccg
     6961 gaggcggtga gcgaagttac tgctatgcaa gtgacgaccc aagaatggac accaaccatt
     7021 tctgcggtcg gttatattcg cccaaatcaa ggggcaatgt taagtgctga aacttcaggc
     7081 gt
//
LOCUS       DANKBV010000119         7020 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.119, whole genome shotgun sequence.
ACCESSION   DANKBV010000119 DANKBV010000000
VERSION     DANKBV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.119"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..652)
                     /locus_tag="PWY43_001971"
     CDS             complement(<1..652)
                     /locus_tag="PWY43_001971"
                     /EC_number="5.99.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484645.1"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2136826.1"
                     /translation="MTTEINYEGIEQMPIKRFTEDAYLNYSMYVIMDRALPFIGDGLK
                     PVQRRIIYAMSELGLNASAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYRY
                     PLVDGQGNWGAPDDPKSFAAMRYTESKLSKIAEILLGELGQGTVDYQPNFDGSLEEPK
                     YLPARLPHILLNGTMGIAVGMATDIPPHNINELADASVMLLDNPKATLDDVLSVVQG"
     gene            837..1874
                     /gene="pstS"
                     /locus_tag="PWY43_001972"
     CDS             837..1874
                     /gene="pstS"
                     /locus_tag="PWY43_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL2136827.1"
                     /translation="MAKRFKLLTVLGISFSLSVAANAQTITGAGASFPYPIYAKWAGL
                     YEKETGNKVNYQSIGSGGGQQQIIAKTIDFGASDDPMKGETLAEHKLLQFPAIIGGTV
                     PVVNIPELKSGELKLSGKVLADIFLGKITQWNDPAIVALNQDIALPAKTILVVRRSDG
                     SGTTFGWTNYLSKVSAEWKEKVGEGKSVKWPVGQGGKGNEGVAAYVKQLKYSIGYVEY
                     AYAKQNALAWVSMQNADGKFVQPSRETFAAAAANADWSQTAGMGVILTNEAGEKAWPI
                     TAASFILIHQHSDKPEQTKGVFKFFDWAFKHGKDAAAELDYVPLPDNVVEKIQTQWKT
                     EVKDGQGNSLY"
     gene            1955..2914
                     /gene="pstC"
                     /locus_tag="PWY43_001973"
     CDS             1955..2914
                     /gene="pstC"
                     /locus_tag="PWY43_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL2136828.1"
                     /translation="MHKHKIDNKCLNHPWVEGLFHHTTRFFALLVFALLVVVLVSLLL
                     GSWEALSRFGVSFLWENEWNPVNDRYGALVPIIGTLISAGIALVIAVPVSFGIATFLT
                     ELAPQWLKRPVGTAVEMLAAIPSIIYGMWGLFVFVPLFQEYVQPYLIDTFGQLPFIGM
                     LFSGAPFGIGLFSAGLVLAIMIIPFIASIMRDVFSVVPPLLKESAYGLGSTTWEVIWK
                     VVLPYTKTGVIGAIMLGLGRALGETMAVTFVIGNAFMLPDSVFSPSTSIASAIANEFN
                     EAGGLQKSALMSLGLILFLITSIVLAASRLLIARMGKQEGKRE"
     gene            2911..3759
                     /gene="pstA"
                     /locus_tag="PWY43_001974"
     CDS             2911..3759
                     /gene="pstA"
                     /locus_tag="PWY43_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL2136829.1"
                     /translation="MKNYQNRRFYCRKIKNVLMLSLAYSAVLFGLFWLVWIIVTLLQK
                     GIPALSWELFTRPTPAPNETGGLRNAIVGSILIVSTATLIATPIGILAGTYLSEYGRY
                     TKLAKFTRFLNDVLLSAPSIIIGLFIYAIYVAQVKHYSGWAGSLALAVLVIPIVVRTT
                     DSMLNLVPNNLREAAAALGCPRWRVITMLSYRAARSGIITGVLLAVARISGETAPLLF
                     TALSNQFASLDMNAPMANLPVVIYQYAASPFADWNTLAWAGAMLITLFVLSLNIITRL
                     YFSKTK"
     gene            3769..4539
                     /gene="pstB"
                     /locus_tag="PWY43_001975"
     CDS             3769..4539
                     /gene="pstB"
                     /locus_tag="PWY43_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDL2136830.1"
                     /translation="MLPTDLETKLHINNLNFYYGDFHALKNINMRIAKNKVTAFIGPS
                     GCGKSTLLRTLNRMFELYPNQRATGEINLDGENLLTSDTDISIIRAKVGMVFQKPTPF
                     PMSIYDNVAFGIRLFEKLPKSELNDRVEWALTKAALWNEVKHKLNQNGDSLSGGQQQR
                     LCIARGIAVKPEVLLLDEPCSALDPISTMKIEELINELKHDYTVAIVTHNMQQAARCS
                     DYTAYMYLGELIEFGDTKQIFDKPKFRQTEDYIKGKMG"
     gene            4623..5309
                     /gene="phoB"
                     /locus_tag="PWY43_001976"
     CDS             4623..5309
                     /gene="phoB"
                     /locus_tag="PWY43_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HDL2136831.1"
                     /translation="MNNKILIVEDERAIREMLSLYLSKQYQVIEAEDYQSAINKLGEK
                     PELILLDWMMPGRSGIHLIKYLKKQPETEKIPIIMLTARSSEEDCITGLNAGADDYIT
                     KPFSPQVLLARIEAVWRRSYDPPSNLINIDGLILDQQAQRVLVAEQEVKLSGTEYKLL
                     HFLITHPEKVYRREQLLDFIWGNDIYVEDRTVDSYIRRLRKSLEPYGFERYIQTVRGS
                     GYRFSNHFQD"
     gene            5312..6592
                     /gene="phoR"
                     /locus_tag="PWY43_001977"
     CDS             5312..6592
                     /gene="phoR"
                     /locus_tag="PWY43_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDL2136832.1"
                     /translation="MKVKLSLKHFLLELLLAAAVAGVFNLFFPAYFFWFSFCLLVLLI
                     WHHHTESSLLQLLRPNEQSGRSKVTTMEHLSQSAAFYHERLRQDKIKTLRLLSKLNKH
                     IQYLPDAIIICQENGEISWCNNVAQELFEFYWDKKSNKSIFNVIFYPEFKSYFRQQRH
                     HRPLVLLTHNKRYIELNLNRYDSNSRLIVARDVTSFVRLMHSRQTFLANMNHELRTPL
                     TILQGYLEMLENEPHNMALQQKALNAMKEQSQRMTNLLRQLSLLVKIEHSNDSEHNEV
                     QMSALISAIQKNTEILSQNQQILFDIEPNLTVWGDQEQLQSAVSNLIYNAIRHAGEGV
                     TIQICCQRDNQEPNTAIFSVQDDGIGIDAHHITHLTERFYRVDESRSNKTGGSGLGLA
                     IVKHALEQHHSTLEIESEAGKGSRFSFKLPLIKK"
     gene            6854..>7020
                     /locus_tag="PWY43_001978"
     CDS             6854..>7020
                     /locus_tag="PWY43_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136833.1"
                     /translation="MTPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVV
                     AKKDVKEGQDF"
BASE COUNT         2028 a         1522 c         1453 g         2017 t
ORIGIN      
        1 gcccttgtac cacgcttagt acatcatcaa gcgttgcttt cgggttatcc agcaacatca
       61 cactggcatc agccaattca ttaatattgt gtggtggaat atcggttgcc attcccaccg
      121 caatccccat cgtgccattc aacaaaatat gcggtaaacg agccggcaga tatttcggtt
      181 cttccaacga gccatcaaaa ttgggctgat aatccaccgt tccttgccct aactcgccca
      241 ataaaatttc tgcaatttta gacagtttcg attcggtata acgcattgca gcgaaggatt
      301 tcggatcgtc cggagccccc cagttgcctt gtccgtccac taacggatag cggtaagaga
      361 acggttgagc cattagcacc attgcttcat agcaggcact atcaccgtgc ggatggaatt
      421 tacctaatac atcccccacg gtacgggctg attttttata ctttgccgag gcgtttagcc
      481 caagttccga catcgcataa ataatacggc gttgcaccgg tttcaagcca tcgccaataa
      541 acggcaatgc ccggtccata attacataca tcgaataatt gaggtaagca tcttcggtaa
      601 aacgcttaat cggcatctgt tcaatgcctt cataattgat ttctgtggtc attttttatc
      661 gatatttata ttaaaaaaac gagggctatt ttctacgatt tagcctgttt agtctagcct
      721 gaaacacaag cggtcgtttt ttctgatttt tttaccattg tcatcatttt gtcatattct
      781 ttttctacac taagcacgtg aaattaactc acgctaactt taaggagaac aatatgattg
      841 ccaaacgatt taaactactt accgttttag gcatttcttt ttcactttct gtcgcagcaa
      901 atgctcaaac catcacagga gccggtgctt ctttccctta tcctatctac gccaaatggg
      961 caggtttata tgaaaaagag accggtaata aagtcaatta ccaatctatc ggttccggtg
     1021 gcggtcagca acaaattatt gccaaaacaa ttgatttcgg tgcttctgat gacccgatga
     1081 aaggcgaaac attagcagaa cacaaacttt tgcaatttcc cgccatcatt gggggaactg
     1141 taccggttgt caatattcct gaacttaaat cgggcgaact gaaactgtcc ggtaaagttc
     1201 ttgccgatat attcctaggc aaaattacac aatggaacga tccggcaatt gtcgcactta
     1261 atcaagatat cgccctgccg gcaaaaacaa ttttagtcgt acgccgctca gacggttcag
     1321 gcacaacttt cggttggaca aactatcttt ctaaagtctc tgccgaatgg aaggagaaag
     1381 tcggcgaagg aaaatcggtg aaatggccgg tcggacaagg cggaaaagga aatgaaggtg
     1441 tcgcagctta tgtgaaacag ctcaagtatt ctattggtta cgtagaatat gcctatgcta
     1501 aacaaaatgc tctggcttgg gtttctatgc aaaatgcaga tggtaaattt gttcagcctt
     1561 cccgtgaaac gtttgctgct gccgctgcta acgccgattg gagccaaaca gccggtatgg
     1621 gcgttatttt aaccaacgaa gcgggagaaa aagcctggcc gattaccgcc gccagtttta
     1681 tcttgattca tcaacatagc gacaaaccag agcaaactaa aggggtattt aaattcttcg
     1741 actgggcatt taagcacggt aaagatgcgg ctgccgaact ggattatgtg cctctaccgg
     1801 ataatgtagt agagaaaatc caaactcaat ggaaaacgga agttaaagat ggacaaggaa
     1861 attcgctcta ctaatgcaaa acgaccgctt gtatggagct taactaacaa caagcggtcg
     1921 ttttttcaca acattttgca aaagagttgt cactatgcat aaacataaaa ttgataacaa
     1981 atgcttaaac catccttggg tagaggggtt atttcatcac acaacccgtt tctttgcttt
     2041 gctggttttt gccttgttgg tggtggtgct ggtttcatta ttgcttggca gttgggaagc
     2101 attaagccga ttcggggtat cattcctatg ggaaaacgag tggaaccctg tgaatgaccg
     2161 atacggtgca ttagtgccga ttatcggcac attgatttct gccggcattg cattagtcat
     2221 tgccgttcca gtctcgttcg gcattgcgac tttcttaacc gaacttgcac cgcaatggct
     2281 aaaacgccct gtcggcacag cggtagaaat gctggcggct attccctcta ttatttacgg
     2341 tatgtggggg ctgtttgtat ttgtaccctt atttcaagaa tatgtgcaac cgtatttgat
     2401 agatacattt ggtcagcttc cctttatcgg tatgcttttt agcggggctc ctttcgggat
     2461 cgggctattc agtgccggct tggtattggc gattatgatt attcccttta ttgcctcaat
     2521 tatgcgtgat gttttcagtg ttgtaccgcc tttactgaaa gagtctgctt acggtttagg
     2581 ctcaaccact tgggaagtta tttggaaagt cgtgctacct tacaccaaaa ccggagtaat
     2641 cggtgcaatt atgctcgggt taggtcgagc attaggcgaa acaatggcag ttacctttgt
     2701 gatcggtaat gcctttatgc tacctgattc ggtattttcc ccttcaacct ccatcgcttc
     2761 tgctatcgcc aatgaattta atgaagccgg cggattacaa aaatcagcgt tgatgagcct
     2821 agggctaatt ctgttcctta ttacttcgat tgttttagcc gcttcacgtt tattaatcgc
     2881 acgaatgggt aaacaggaag gaaaacgaga atgaaaaatt accaaaaccg acgcttttat
     2941 tgccgaaaaa taaaaaatgt cttgatgtta agtcttgcct attccgctgt attatttggc
     3001 ttattttggc tggtctggat tattgttact ttactccaaa aaggtatccc tgcactgtct
     3061 tgggaactgt ttacccgccc gactcctgca ccaaatgaaa caggaggatt acgtaatgca
     3121 attgtcggct ctatactgat tgtcagtacc gcaacactga ttgcaacacc aatcggtatt
     3181 ttagccggca cttacttgag cgaatacggg cgttatacca aattggcaaa attcacccgt
     3241 tttttgaatg atgtgttgct ctctgctcct tctattatta tcggcttatt tatttatgcg
     3301 atttatgtcg cacaggttaa acactattca ggctgggcag gctcattagc attggcggta
     3361 ttagtgattc cgattgttgt tcgaactacc gacagtatgc tcaatcttgt gcctaataat
     3421 ctgcgtgaag cagctgcggc tctcggctgc ccacgatgga gggtaattac aatgctctct
     3481 tatcgtgccg cccgctccgg cattattacc ggcgtgttgc tcgctgttgc ccgtatttcc
     3541 ggagaaaccg caccgctatt gtttaccgca ttatccaacc agtttgcctc tttagatatg
     3601 aatgcaccaa tggcaaacct ccctgtggtg atttatcaat acgcagccag cccgttcgct
     3661 gactggaata cattggcttg ggcaggtgca atgcttatta ctctgtttgt gctttctctt
     3721 aatatcatca ctcgtctgta tttcagcaaa acaaaataag gactctatat gttaccaacg
     3781 gatttagaga caaaattaca tattaacaac ctcaattttt attatggcga tttccacgct
     3841 ctgaaaaata tcaatatgcg tattgccaaa aataaagtga ccgcatttat cggtccttcc
     3901 ggttgtggaa aatcaacatt actgcgaacc ctaaaccgaa tgtttgagct ctacccgaat
     3961 caacgtgcaa cgggggaaat caatcttgat ggagagaatc tactcacctc cgatacggat
     4021 atttcgatta ttcgagccaa agtcggtatg gttttccaaa aaccgacccc attcccgatg
     4081 tccatttacg ataatgttgc ttttggtatt cgtctgtttg aaaaattgcc gaaatccgag
     4141 ctaaacgatc gtgtagagtg ggcattaacc aaagcggctt tatggaatga ggtaaagcat
     4201 aaattaaacc aaaatggtga cagtctctcc ggcgggcaac agcaacggct ctgtattgca
     4261 cgaggtattg cagtgaaacc cgaggtgtta ctgctggatg agccctgctc cgcacttgac
     4321 ccgatttcga ctatgaaaat cgaagaactt atcaacgaac tcaagcacga ctacacggta
     4381 gcgatagtta cccacaatat gcagcaagca gcccgctgtt ctgactatac cgcttatatg
     4441 tatctaggcg agctgattga attcggtgat accaaacaaa ttttcgacaa accaaaattc
     4501 cggcagacgg aagattacat taaaggtaaa atggggtaat ctgcaagcgg tcggttttag
     4561 taaaaaattt gcaaatttct gcgataaatc gaccgcttgt cacaccgcat cggagaggaa
     4621 gaatgaataa caaaattctg atcgttgaag acgaacgtgc tattcgtgaa atgctaagtc
     4681 tctatctcag caaacaatat caggtaattg aagcggaaga ctaccaaagt gctatcaata
     4741 aactgggcga aaaacccgag cttattttac tggattggat gatgccgggg cgttcgggta
     4801 tccatttaat taaataccta aaaaaacagc ctgaaaccga aaaaattcca atcattatgc
     4861 taacagcccg cagtagcgaa gaagactgta ttaccggtct gaatgccggt gcagacgact
     4921 acattaccaa accgttttca ccacaggtgt tgcttgcccg gattgaagca gtatggcggc
     4981 gaagctatga tccgccaagc aatctcatca atatagacgg attgattctt gatcaacagg
     5041 cacaacgggt tttggttgcc gaacaggaag tgaaattaag cggcacggaa tataaactgc
     5101 ttcacttttt aattactcac cctgaaaaag tgtatcgtcg tgaacagttg cttgatttta
     5161 tttggggtaa tgatatttat gttgaggatc gcacggtaga ctcttatatt cggcgattgc
     5221 gtaagagtct tgaaccctat ggttttgagc gttatattca aaccgtacgt ggctcgggat
     5281 accgtttttc caaccatttt caggactaat aatgaaagtt aagctctcac ttaaacactt
     5341 tttattggaa cttttgctgg cagcagccgt tgccggcgta ttcaatctgt ttttccctgc
     5401 ttattttttc tggtttagtt tttgtttact ggttctactt atttggcatc accacaccga
     5461 atcttctctc cttcagttac tcagacctaa tgagcaaagc ggcaggtcaa aagtaacgac
     5521 aatggagcat ttatctcaaa gtgccgcatt ctatcacgaa cgtttacgcc aagataaaat
     5581 caaaacctta cgtctgttat caaaattaaa taagcatatt caatacttac ccgatgcgat
     5641 cattatttgc caagaaaacg gggagatttc ttggtgcaat aatgttgcac aagagctatt
     5701 tgagttttat tgggacaaaa aatcaaataa aagcattttt aatgtgattt tttatcctga
     5761 atttaaaagc tatttccgcc aacaacgaca ccatcgccca ttggtactgc tgactcataa
     5821 taaacgctat attgaattga atctcaatcg atatgacagt aactccagac ttattgtcgc
     5881 acgagatgta acctcttttg tccgccttat gcactcacga caaacctttt tggcaaatat
     5941 gaaccacgaa ctgcgaacgc ctcttaccat attacaaggt tatttggaaa tgctggaaaa
     6001 cgagccgcat aatatggcat tacagcaaaa agcgctcaat gcgatgaaag agcaaagtca
     6061 acgtatgact aacctgcttc gccaactctc acttttggtt aaaatcgagc attccaatga
     6121 ttccgaacac aatgaggtcc agatgtcggc gttaatctcc gccatacaaa aaaataccga
     6181 gattttaagc caaaatcagc aaatactgtt tgatattgag cctaatttaa cggtatgggg
     6241 agatcaagag caactccaaa gcgctgtatc aaatttaatt tacaatgcaa tacggcacgc
     6301 cggtgaagga gttaccattc aaatatgttg tcaacgtgat aatcaagagc cgaatactgc
     6361 gattttcagc gtgcaagatg acggtattgg tatcgatgcc catcatatta cccatctgac
     6421 agaacgtttc tatcgtgtgg acgaatctcg tagcaacaaa accggcggta gcggtttagg
     6481 tctggctatt gtcaaacacg ctcttgaaca gcaccattcc acactggaaa ttgaaagtga
     6541 agccggtaaa ggaagcagat ttagttttaa actacctcta ataaaaaaat agcgatgcaa
     6601 tcaaactatt ttcacaaggc ttttcacaaa ctaatcccca ttttacactg aagttttgaa
     6661 tttctttata actgaggtgc aagatcaaaa taataggcga ttttttataa aaatcacgct
     6721 atactagaca agttttattt cttgttattg agaggcggct atcaaaaatg ttttacttaa
     6781 aagccttttt gctagtcgca atttagagaa ataaaacgac aataatagta aattctttaa
     6841 aaggaaaatt tcaatgactc caattgtaaa acagtttaaa tacggtcagc acaccgtgac
     6901 cttagaaacc ggtgctatcg cacgccaagc aacggcagca gtaatggcaa gtatggacga
     6961 cacaaccgta tttgttaccg tagtagcgaa aaaagacgta aaagaagggc aagatttctt
//
LOCUS       DANKBV010000120         7016 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.120, whole genome shotgun sequence.
ACCESSION   DANKBV010000120 DANKBV010000000
VERSION     DANKBV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.120"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..975
                     /locus_tag="PWY43_001979"
     CDS             <1..975
                     /locus_tag="PWY43_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2136834.1"
                     /translation="QGPYKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWKAEN
                     PGKPFPVSVAIGADPATILAAVTPVPDTLSEYAFAGLLRGHKTEVVKSISNELEVPAS
                     SEIVLEGYIDLEETALEGPYGDHTGYYNEQEYFPVFTVTHITMRKDAIYHSTYTGRPP
                     DEPAVLGEALNEVFIPILQKQFPEIVDFYLPPEGCSYRLAVVTMKKQYAGHAKRVMMG
                     VWSFLRQFMYTKFVIVCDDDVNARDWKDVIWAITTRCDPARDTTLIENTPIDYLDFAS
                     PIAGLGSKMGIDATNKWAGETSREWGTPIKKDDNVVKRIDEIWDSLGI"
     gene            complement(1059..1883)
                     /locus_tag="PWY43_001980"
     CDS             complement(1059..1883)
                     /locus_tag="PWY43_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2136835.1"
                     /translation="MDSLTHLFTQFQFRTDLFHLGQLCQIGTFNEENKGYLHFVRQGR
                     FILNQAAERPITIERPAIIFSPTNILHSLHPLDDSGLDIFCINFDFGKGIRNPLTHTL
                     HNIVILELAQYPELGVLADQIFSEVNQKGCGYQAMIHHLCAYFTLKVARQCLEKGYIQ
                     TGLLKGLADKQLGKVLLAIHSAPERDWNVEELANVALMSRSRFAAYFKQIMGVSPMDY
                     LTNWRVAVAQHLLQKGIPVALVAEQVGYSHNAALSRVFMRELGLSPTEWLAQNKES"
     gene            2010..2855
                     /locus_tag="PWY43_001981"
     CDS             2010..2855
                     /locus_tag="PWY43_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2136836.1"
                     /translation="MMSQEQFEEINQERRQALNHGSRIAMAASVLGALGIASTANAKQ
                     ASAEKSPYAEPEQYGLKRHGMTIDPKRVALVVVDPQIDFLSPKGVMWGVLGDSIKENN
                     TVANIESLFKAAKAVDMPTFVSSHYYYPTDHKWEFGSPGEHFMHDSGMFARKGQYTME
                     GFENSGADFMPEYKPYIFDGKTVIASPHKIFGPETNDLVLQLRKRKIDQVILAGMAAN
                     LCIDSHLRELIEQGFEVTVVKDATAGPRIPEGDGYLAALTNYRIIANDLWTTEEAVKK
                     MTAKA"
     gene            3312..6254
                     /gene="rne"
                     /locus_tag="PWY43_001982"
     CDS             3312..6254
                     /gene="rne"
                     /locus_tag="PWY43_001982"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDL2136837.1"
                     /translation="MKRMLINATQKEELRVALVDGQRLFDLDIESPGHEQKKSNIYKG
                     KITRVEPSLEAAFVDYGAERHGFLPLKEISREYFPSDYVFNGRPNIKDIIQEGQEVIV
                     QVSKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERLELKEALDCLDV
                     PEDVGLIVRTAGVGKSPEELQWDLKVLLHHWEAIKKAAESRPAPFLIHQESDVIVRAI
                     RDYLRRDIGEILIDNKKVFEKAKNHIRLVRPDFINRVKLYEGEVPLFSHYQIESQIES
                     AFQREVRLPSGGSIVIDVTEALTAIDINSSRSTRGGDIEETALNTNLEAADEIARQLR
                     LRDLGGLIVIDFIDMTPVRHQREVENRIREATRQDRARIQFARISRFGLLEMSRQRLS
                     PSLSEASSHICPRCQGTGKVRDNESIALSILRLLEEEAIKENTAQVHTIVPVQIASYL
                     LNEKRKAISSIEKRHDVQIVVVPSESMETPHFSVYRLRENEITPTLSYDLTKHYEARN
                     EEEHPHHHHQEDVLVTRNEPVITVESVLQSSELNLQPSPTPTEPPKPSLLSRLIATIK
                     GWFSEEKVEEKPKPKARNQRDRRQRRERPNRNTKAENQERKTVQAQATPAEEKPVRSE
                     KKARRAVVEEVSGVTEQESRSNSREVQTERRQRRDLRKKVRVETQDVEAENLTPAVEE
                     TVAKQVENVQKNQQIQPLATQELPVETVPVVETTNEGRENNRQRRLPRHLRVGNQRRR
                     DNQRNQIASPMPLTSAVASPELASGKVWVQVTSTVKVEEKASFLSVDQMLEQQENQLV
                     ETEKKSEEKKLHTVSPLGGFVAQATNRDLVKEKQEQLSKPKEEKVASEDVKPVEVKEH
                     IAPFGSVVAQHSERDLVKEKNERVSKFQSQATVAPAIVEEVTNPIEVPKSTYESDYRF
                     NGRLGTFSRVEHTQAAMTIASAPSDKLEVVEISQWQNSKYYFYGKGFGGYTSAVSQVQ
                     SAPRSTNG"
     gene            6400..6960
                     /locus_tag="PWY43_001983"
     CDS             6400..6960
                     /locus_tag="PWY43_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136838.1"
                     /translation="MKKSFLSLLVLGTTMALAACDQAKEAAQSTTDKAVEVKEAVAEK
                     AAEVKSATEAKVEELKAAANDAAQATADKTEEAKKAVAEKATELKTTTEAKVEELKAA
                     ANDAAQATTDKTEEAKKAVAEKAAELKATTEAKVEELKAAASDATQATADKAEEAKKA
                     VAEKAAEVKVETETKVEEMKKAIESK"
BASE COUNT         2275 a         1494 c         1539 g         1708 t
ORIGIN      
        1 caagggcctt acaaaaaacg ccagaattta ggcatttatc gtcagcaact tatcggcaaa
       61 aacaaactga ttatgcgttg gctttcccac cgtggtggtg cgttggactt ccaagagtgg
      121 aaagccgaaa acccgggtaa accgtttccg gtgtcggttg cgattggtgc agatccagcc
      181 actattttag cggcagtcac ccctgtgccg gatacgcttt cggaatatgc gttcgcaggg
      241 ctgttgcgag ggcataaaac cgaagtggtc aaatctattt ccaatgagtt agaagtgccg
      301 gcaagtagcg aaattgtgct ggagggctat atcgatctgg aagaaaccgc attggaaggc
      361 ccttatggcg atcacaccgg ctactacaac gagcaagagt attttccggt atttaccgtt
      421 acccatatta ctatgcgaaa agatgcgatt tatcactcca cttacaccgg cagaccacct
      481 gatgaaccgg cagtgttagg cgaggcgtta aacgaagtct ttattccgat tttgcaaaaa
      541 cagtttccgg aaattgtcga tttctacttg ccacctgaag gctgttctta ccgtttagcg
      601 gtggtgacaa tgaaaaaaca atatgccgga cacgccaaac gggtgatgat gggggtttgg
      661 tcgttcctac gtcaatttat gtacaccaag tttgtgattg tgtgcgatga tgatgtgaat
      721 gcccgagatt ggaaagacgt gatttgggcg attactacta ggtgcgatcc ggcacgagat
      781 accacactca tcgaaaatac ccctattgac tatctggatt ttgcctcacc gattgccgga
      841 cttggctcaa aaatggggat tgatgccacc aataaatggg cgggtgaaac ctcaagagag
      901 tggggaacgc caatcaaaaa agatgacaat gtggtgaaac gcattgatga aatttgggat
      961 tctcttggca tttaaggcaa catttaaccg cttgtgcaac ataacaagcg gttaaatttc
     1021 tgatattttt accattctgc tctttattgc caatcaggtt acgattcttt attttgagct
     1081 aaccactcgg taggcgaaag ccctaattcc cgcataaaga cacggctgag agcagcatta
     1141 tggctataac cgacttgctc tgccaccaat gctaccggta tccctttttg taataaatgc
     1201 tgggcaaccg ctactcgcca gttagtgaga taatccatcg gtgaaacccc cataatttgt
     1261 ttaaaataag cagcaaagcg tgagcgagac attaatgcca catttgccaa ctcctctaca
     1321 ttccaatctc gttccggtgc actgtgaata gctaacagca ctttccccag ttgcttatct
     1381 gccaagcctt ttaacaaacc tgtttgaata tacccttttt ctaaacattg acgagcaacc
     1441 ttcaaggtaa aataggcaca taaatggtgg atcattgcct gataaccaca ccctttctga
     1501 tttacttcac taaaaatttg atcggctaat acccccaatt ccggatactg tgccaactct
     1561 aagatcacaa tattgtgcaa ggtatgcgtt aatgggttgc gaatcccttt gccaaaatca
     1621 aaattaatgc agaagatatc taacccacta tcatctaacg ggtgcaaact gtgcaaaata
     1681 ttggtcggtg aaaaaataat cgctgggcgt tcaatcgtaa tcgggcgttc agcagcctga
     1741 tttaaaataa aacgtccttg gcgaacaaaa tgtaaatagc ctttattctc ttcattaaaa
     1801 gtcccgattt ggcacaactg acctagatga aataagtccg tacgaaactg aaattgggta
     1861 aaaagatgag tcaaactatc cacattaagc tccttgggta aacaattgag acgaacggtt
     1921 aaaacaagcg gaagggagtc attataatag caacccgttc ggtaacgcaa gttatcatta
     1981 caatttgtat atctattaat gaggataaaa tgatgtcaca ggaacaattt gaagaaatca
     2041 accaagaacg ccgtcaagcc cttaaccacg gctctcgtat tgcaatggca gccagtgtat
     2101 taggtgcatt aggtattgca tcaacagcaa atgcaaaaca agccagtgca gaaaaatcac
     2161 cttatgccga gccggaacaa tacggtttaa aacgccatgg aatgactatt gatccaaaac
     2221 gtgtggcatt agtggtagtt gatccacaaa ttgatttcct aagcccgaaa ggcgtgatgt
     2281 ggggcgtgtt aggtgattca atcaaagaga acaacacggt agcgaatatt gaaagtttat
     2341 ttaaagcagc aaaagcagta gatatgccaa cctttgtttc gtcacactac tattacccaa
     2401 ccgatcacaa atgggaattt ggttcaccgg gcgaacactt tatgcacgac agcggaatgt
     2461 ttgcaagaaa aggtcaatac accatggaag gctttgaaaa ttcaggtgca gactttatgc
     2521 ctgagtacaa gccatatatt tttgatggca aaacagtgat cgcctctcca cacaaaatct
     2581 ttggaccgga aaccaatgac ttagtgctgc aattacgcaa acgtaaaatc gaccaagtaa
     2641 ttttagccgg tatggctgcc aacttatgta ttgattcaca cttgcgtgaa ttaatcgaac
     2701 aaggttttga agtaacagtg gtgaaagatg caaccgcagg gcctcgtatt cctgaaggtg
     2761 acggctacct tgcagcatta accaactacc gtattatcgc aaatgacctc tggaccactg
     2821 aagaagcagt gaaaaaaatg acggcaaaag cataatattc gctttacaag cggttaaatt
     2881 tagctctttt tttgcaaatt taaccgcttg gatttcacgc ttatctccag cactcacact
     2941 aaatctcatt atttttcctt atcaccccat tcattttcat tatattcatc tccacaacaa
     3001 ataacagcta ccatacaaca attaccttgc ctttttcagg gttaaaatgc gataatgact
     3061 cgttattttt ggcttgaaaa agcaatagaa aattgcaaaa aataaagcaa aaataaccgc
     3121 ttgtaataga gataaacaag ccttaagtga ttgattttgc agtttcttta agacaagaca
     3181 aagtaaacgt aagacaacca tcaatgcact atcgggaatg aagacagtcg tgagatagac
     3241 attaaatcca ttcccttatt aatgggtcaa aatgaccgtg taagacaaaa acaaaataat
     3301 gagaaaacac aatgaaaaga atgttaatca atgcaactca aaaagaagag ttgcgtgttg
     3361 ccctcgttga tgggcaacgc ttatttgact tggacattga aagcccgggt cacgaacaga
     3421 aaaaatccaa tatctacaag ggtaaaatta cccgtgtcga accaagcctt gaagctgcat
     3481 tcgtagatta cggtgcagaa cgccacggct tcctaccttt aaaagaaatt tcccgagaat
     3541 acttcccgtc agactatgtg tttaacggtc gcccgaatat caaagatatt attcaagaag
     3601 ggcaagaagt gattgttcaa gtcagcaaag aagagcgtgg caacaaaggg gctgccttaa
     3661 ccacttttat ttcgcttgcg ggcagttact tagttttaat gccgaacaac cctcgtgccg
     3721 gtggtatttc ccgccgtatt gaaggcgatg agcgtttaga actcaaagag gcattagact
     3781 gcttagatgt gccggaagat gtcggcttaa tcgtgcgtac cgccggtgtc ggcaaatcgc
     3841 cggaagagtt acaatgggac ttaaaagtct tgttacacca ctgggaagcg attaaaaaag
     3901 cggcggaatc tcgccctgct ccgttcttaa ttcaccaaga aagtgacgtg attgtgcgtg
     3961 caattcgtga ctatctacgc cgtgatattg gcgaaatctt aatcgacaac aaaaaagtgt
     4021 tcgagaaagc caaaaaccac attcgccttg ttcgcccgga tttcatcaac cgtgtgaaat
     4081 tatacgaagg cgaagtacca ctattcagcc actatcaaat tgaatcgcaa atcgaatcgg
     4141 cgttccaacg tgaagtgcgt ttaccttccg gtggctctat cgtcattgat gtcacagaag
     4201 ccttaaccgc gattgatatt aactcttcac gctcgacccg tggtggcgat attgaagaaa
     4261 ctgcactcaa tactaactta gaggcagccg atgaaattgc tcgtcagtta cgtttacgtg
     4321 acttaggcgg tttgattgtg atcgacttta tcgatatgac gccggttcgc caccaacgcg
     4381 aagtggaaaa ccgtattcgt gaggcaaccc gccaagaccg tgcgagaatc caatttgccc
     4441 gtatttcccg tttcggtttg ttagaaatga gccgtcagcg tttaagccca tcattaagtg
     4501 aggcttcatc gcatatctgc cctcgctgtc agggaacggg taaagtacgt gataatgaat
     4561 caatcgcact gtcaatttta cgtttattag aagaagaggc aatcaaagaa aacactgcac
     4621 aggttcacac cattgtgccg gtgcaaatcg ccagctactt actcaatgaa aaacgtaaag
     4681 cgatttccag cattgaaaaa cgccacgatg tacaaattgt tgtagtaccg tctgaaagta
     4741 tggaaacacc gcatttcagc gtatatcgcc tgcgtgaaaa tgaaattacc ccaacgttaa
     4801 gctacgattt aaccaagcat tatgaagctc gcaatgagga agaacaccca catcatcacc
     4861 atcaagaaga tgtgctggtt acccgtaacg agccggtgat tacggtagag tctgtgttac
     4921 aaagctcgga gctgaatctc caaccttcgc caacacctac cgaaccacca aaaccatcgc
     4981 tattaagccg cttaattgcg accattaaag gctggtttag cgaagaaaaa gtggaagaaa
     5041 aaccgaagcc gaaagcacgc aaccagcgtg atcgtcgcca acgccgtgaa cgcccgaacc
     5101 gcaataccaa agcggaaaac caagagcgta aaaccgtgca agcgcaagct actccggcgg
     5161 aagaaaaacc ggtgcgtagc gagaaaaaag cacgccgagc ggtagtggaa gaagtttccg
     5221 gcgtaaccga gcaagagagt cgtagcaact ctcgtgaggt tcaaaccgaa cgccgccaac
     5281 gtcgcgacct acgcaaaaaa gtgcgtgttg aaacacaaga cgtggaagca gaaaacctca
     5341 cgccagcagt tgaagaaacc gttgcgaagc aagttgaaaa tgtgcaaaaa aatcagcaaa
     5401 ttcaaccgct tgcaactcaa gagttgccgg ttgaaacagt tcctgtggta gaaacaacga
     5461 acgaaggacg tgaaaataac cgccaacgcc gtttacctcg ccatttgcgt gtgggtaatc
     5521 aacgtcgtcg tgataatcaa cgtaatcaaa tagcttcgcc tatgcctctg acatcagcgg
     5581 ttgcttcgcc ggagcttgcc agcggtaaag tttgggttca ggttacctct actgtcaaag
     5641 tagaggaaaa agcatcattc ttatctgttg atcaaatgct tgaacagcaa gaaaatcagc
     5701 ttgttgagac agagaaaaaa tcagaagaga agaagcttca tacagttagt ccgctaggtg
     5761 gctttgtagc acaagcaact aaccgtgatt tggttaagga gaagcaagaa cagctttcca
     5821 aaccgaaaga agaaaaagtt gcttctgaag acgtaaaacc agtagaggta aaagagcata
     5881 ttgctccatt tggtagtgtt gttgctcagc attctgaacg tgatttagtt aaagagaaaa
     5941 atgagcgagt cagtaaattc caatcgcaag caacggttgc accagcgatt gtggaagagg
     6001 ttactaaccc gattgaagtg ccaaaatcaa cttacgaaag cgattatcga tttaacggca
     6061 gattaggtac attctctcgt gttgagcata cccaagccgc tatgaccatt gcttctgcac
     6121 caagtgacaa gcttgaagta gtcgaaatta gccaatggca aaactctaaa tactatttct
     6181 atggcaaagg gtttggcgga tataccagcg cagtgagcca agtgcagtca gcgcctcgtt
     6241 caaccaatgg ttaagtacaa ttaattggtt taaatgccta ttagttataa ctaataggca
     6301 tttttttatc aaagtatgaa ctttctatca atttttctgc ctaagtaaac gcactttatt
     6361 acaaagtgcg ttatttttag tttatttaag gaactcggta tgaaaaaatc atttttatca
     6421 ttattagtgt taggtacaac tatggcactt gctgcttgtg atcaggcaaa agaagccgcg
     6481 caatcaacta cagacaaagc ggttgaagta aaagaagctg tagctgaaaa agctgcagaa
     6541 gttaaatcgg ctactgaagc gaaggttgaa gagttgaaag cggcagcaaa tgatgcagct
     6601 caagcaacag cagataaaac agaagaggca aaaaaggcag tggcagagaa agccactgag
     6661 ttaaaaacca caactgaagc taaagtggaa gaactaaaag cagcagcaaa tgatgcagct
     6721 caagcaacaa cagataaaac agaagaggca aaaaaggcag tagcagagaa agctgctgag
     6781 ttaaaagcca caactgaagc taaagtggaa gaactaaaag cagcagcaag tgatgcgact
     6841 caagcaacag cagataaagc agaagaagct aaaaaggcag tagctgagaa ggcagcagaa
     6901 gtgaaagttg agacagaaac aaaagttgaa gaaatgaaaa aagcgattga aagcaaatag
     6961 tgctttttag tttctctgta gctatcgaaa cacgctaaaa ataaagcctg gattat
//
LOCUS       DANKBV010000121         6992 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.121, whole genome shotgun sequence.
ACCESSION   DANKBV010000121 DANKBV010000000
VERSION     DANKBV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.121"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..601)
                     /locus_tag="PWY43_001984"
     CDS             complement(<1..601)
                     /locus_tag="PWY43_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDL2136839.1"
                     /translation="MKLTKIALSLTALVVSSMSVAGTLSTSSMVDILAFDGQRVKSGT
                     TSVQMSENKTHQVVVEVGGTVEGDFFTSDQIILTFQGSAENAKLETPRLNSKLDLRKF
                     QENQTVAIKTDSGKVISHKQDFLKGEGFLAKTRVEDNLTKYNLAKKTASVEQFATAPL
                     EAKGQIVVDTNKVSQEELQLLFRKADKDTQKRFLEWAKKN"
     gene            739..1638
                     /gene="corC"
                     /locus_tag="PWY43_001985"
     CDS             739..1638
                     /gene="corC"
                     /locus_tag="PWY43_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249295.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDL2136840.1"
                     /translation="MSDEQQSVNTTEKSEKKSILHSLFSGLFQNEPKNREDLVEVIRD
                     SVENELIDSDTKEMIEGVMEISELRVRDIMIPRAQIVFIDANEPLDVCIDLIIESAHS
                     RFPVIRGEKDTIEGILHSKDLLKYLRSDSEPFNLQQVLRPAVIVPESKRLDRMLKEFR
                     SERFHMAVVVDEFGAVSGLVTIEDILEQIVGDIEDEFDEEEVKPIRQLSRHTYAVLPL
                     TDIKKFNEFFATDFNDEEVDTIGGLVMQAFGYFPKRDEQIELNGIDFKVTSANSRQLI
                     QLRVTLSDEQLEKMEKTSPNEEE"
     gene            1697..3205
                     /gene="lnt"
                     /locus_tag="PWY43_001986"
     CDS             1697..3205
                     /gene="lnt"
                     /locus_tag="PWY43_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDL2136841.1"
                     /translation="MNFAKNPPFLTACLLAIILGAIGTFAYSPFDIWILAFASAAGLV
                     WSATLANRKTALWATFAWSIGYFCTGVNWVSVSMTQFGGVPTIVSYIAVFLLACYLAI
                     YNLLFSVVSHRLKLNNPFALAAIFTFTEYLRGVVFTGFPWLQFGYSLIDSPFAGIAPI
                     LGVEGLTFLVIVASSYLVKIIQQENKTVPSLMVLAVIFGLSFATRFLQFVQIDTEKQP
                     LVVSLVQGNIEQKMKWDPQHFNHSVRTYEQLILPLLGNSELIILPESAIPALENQISP
                     LLGSLDHLARQHGSEIIIGTLYQNEQEELFNSSVLLGEKEREYALHSNPRYNKHHLVP
                     FGEYVPFGSVLDWMREVFILPINLSQGSFVQPSFLAKNNRFNMAICYEIIFGDQLQQN
                     QKSQNSDYLLTITNDAWFGSSIGPWQHFQMARMRALELGKPLLRAANTGITAIVDANG
                     KVIEQLPQFTADALTATIVPTKGDTPFKQLGHWLIYSFSLFCVIASLLLRRK"
     gene            complement(3251..3715)
                     /locus_tag="PWY43_001987"
     CDS             complement(3251..3715)
                     /locus_tag="PWY43_001987"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDL2136842.1"
                     /translation="MATFQGNYIATGLKFGIVATNWHKIFIDQLLNGATDKLLRHGVE
                     EKNIDTVWVPGALEISIAAKKMAQSGRYDAIICLGAVVRGATSHYDVVVNESAKGISS
                     SALETGVPIINGILTVENLEQAIERSGTKAGNKGEECAMVAIEMANLLKALA"
     gene            complement(3921..5120)
                     /locus_tag="PWY43_001988"
     CDS             complement(3921..5120)
                     /locus_tag="PWY43_001988"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDL2136843.1"
                     /translation="MFKFSTVEEAIQAIAQGKIILVSDDADRENEGDFICAAEFATPE
                     NINFMAKYGKGLICTPISTEIAKKLNFHPMVLENEDNHQTAFTVSVDHIQTGTGISAF
                     ERSLTCLKMLDENAKPEDFRRPGHVFPLVAKDGGVLVRNGHTEATVDLARLAGLKPAG
                     LCCEIMAEDGTMMQMPELQAFAQAHNMPFITIQQLQEYRRKHDSLVNVVSVVKMPTKY
                     GEFKAHSFVETISGKEHVALVKGDIGDGENVLCRIHSECLTGDAFGSQRCDCGQQFAA
                     AMNQVEQEGRGVVLYLRQEGRGIGLINKLRAYELQDRGMDTVEANLALGFKDDEREYY
                     IAAQMFEKLGVKSIRLLTNNPAKIEGLIEQGLNVVAREPIMVEPNEHDLEYLKVKQHK
                     MRHMFNF"
     gene            complement(5139..5786)
                     /gene="ribE"
                     /locus_tag="PWY43_001989"
     CDS             complement(5139..5786)
                     /gene="ribE"
                     /locus_tag="PWY43_001989"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDL2136844.1"
                     /translation="MFTGIIEEVGQIAQIKKQGEFVVVTVKAKKILTDVHLGDSIAVN
                     GVCLTVTSFTQEQFTADVMSETLKRTSLGELSINSSVNLERAMAANGRFGGHIVSGHI
                     DGTGVVAEITPADNSTWYRIKAEPKLMRYIIEKGSITIDGISLTVVDVDAESFRVSII
                     PHTIKETNLGTKKIGSLVNLENDIVGKYIEQFLLKKEPENPQSKITADFLKNAGF"
     gene            complement(5968..>6992)
                     /gene="ribD"
                     /locus_tag="PWY43_001990"
     CDS             complement(5968..>6992)
                     /gene="ribD"
                     /locus_tag="PWY43_001990"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDL2136845.1"
                     /translation="LVGCVIVKNGNIIAEGYHQKAGSWHAERNAILNCEEDLTGATAY
                     VTLEPCCHHGRTPPCSELLIERGIKKVFIGSRDPNPLVSGKGAAQLRAAGIEVVEDFM
                     RTECDALNPIFFHYIQTKRPYVLLKYGMTADGKIATYTGNSKWITGEAARANVQKSRH
                     EYSAIMVGVETVLADDPMLNSRMENAKQPVRIVCDSQLRTPLDSQLVQTARQYRTIIA
                     TLNNDAKKQAEYTACGVEILVTGERDKRVDLADLLQKLGELGIDSLLIEGGSQLNFSV
                     LEQGLVNRVHCYIAPKLIGGKAAKTPIGGKGIGELAQAVKLRLKSTELLGEDILLDYE
                     VDLLRN"
BASE COUNT         1882 a         1598 c         1348 g         2164 t
ORIGIN      
        1 tatttttctt cgcccattct aagaaacgtt tttgggtgtc tttatctgcc tttctaaata
       61 aaagctgtaa ttcttcttgt gaaactttat ttgtatccac cacaatttgt ccttttgctt
      121 caagaggtgc cgtcgcaaac tgttcaactg aagctgtttt tttcgctaag ttatatttcg
      181 ttaaattatc ttcaactctt gttttcgcaa ggaaaccttc cccttttaag aaatcttgtt
      241 tatgtgaaat aaccttacct gaatcggttt taattgccac agtctgattc tcttggaatt
      301 tacgaagatc taactttgaa tttaatcttg gcgtttctaa ttttgcattt tccgcactgc
      361 cttggaaagt caaaatgatt tgatcagagg tgaaaaaatc cccttcaact gtcccaccga
      421 cttccactac cacttgatgc gttttattct cactcatttg aaccgaagtc gtgccggatt
      481 taacacgttg cccgtcaaag gctaaaatgt ccaccattga tgatgtactt aacgtcccgg
      541 ccacactcat cgaactgaca actaaggctg ttaaacttaa tgctattttg gttaatttca
      601 tttcaaactc ctaaaatatg aagaaataaa aagatttttg cttatcctat gctaatttga
      661 gtgaaaatag caatgtttta tttgctttgt gactgaattt gcttattata agcgtacttt
      721 atttaagtgg aacaaactat gagtgatgaa cagcaaagtg taaacaccac cgaaaaatca
      781 gaaaaaaaat cgattttaca ctctctattt agcgggcttt ttcaaaacga gcctaaaaac
      841 cgtgaagatc tagtcgaagt gattcgagac tctgttgaaa atgaattgat cgacagcgac
      901 accaaagaga tgatcgaagg agtaatggaa atctcggaat tacgagtgcg ggatattatg
      961 attccccgtg cgcaaattgt gtttattgat gccaatgaac ctttagatgt ctgtattgat
     1021 ttaattattg aatccgcaca ctctcgtttt ccggtgattc ggggtgagaa agatactatc
     1081 gaaggtattc ttcactctaa agatttattg aaatatttac gttctgactc tgaacccttt
     1141 aatctgcaac aggtattacg ccctgcggtt attgtaccgg aaagcaaacg cctagaccga
     1201 atgctaaaag agttccgttc agaacgcttc catatggcag tagtggttga tgaatttggt
     1261 gcggtttcag gcttagtcac cattgaagac attcttgaac aaattgtggg tgatattgaa
     1321 gatgagtttg atgaagaaga ggtcaaacca attcgccagc tttcaagaca tacttatgcg
     1381 gtattaccgc tcaccgacat caaaaaattc aatgaatttt ttgctaccga ctttaatgac
     1441 gaagaggtgg ataccattgg tggtttagtg atgcaggctt tcggttattt cccgaaacga
     1501 gatgagcaaa ttgagttaaa cggaattgat tttaaagtca cctcagctaa tagccgccaa
     1561 ttgattcagc tacgagtcac actttccgat gaacagttag agaaaatgga aaaaacctct
     1621 cctaacgaag aagaataata tagtgagttt caatcaaggc acgtttcggc gtgcctcatt
     1681 tatttagtga gttattatga attttgcaaa aaatccgcct tttttaaccg cttgtctcct
     1741 tgcaatcata ctgggtgcaa tcggcacttt cgcctattcg ccttttgata tttggattct
     1801 tgcctttgct tccgcagccg gcttggtttg gtcagccact ctggcaaaca gaaaaaccgc
     1861 attatgggca acctttgctt ggtctatcgg ctacttctgc accggtgtaa actgggtttc
     1921 tgtgagtatg acccaatttg gtggtgttcc taccatagta agctacatag cagtattcct
     1981 gttggcgtgc tatctcgcta tttataatct gctgtttagt gtagtttcac acaggctcaa
     2041 actgaataat ccgtttgcat tggctgcgat ttttaccttt accgaatatt tacgaggcgt
     2101 ggtatttacc ggttttccgt ggctacaatt cggctatagc ttaatagaca gcccctttgc
     2161 tggtattgcg ccgattttgg gcgttgaagg tttgactttc ttggtaatag ttgcaagtag
     2221 ctatctagtg aaaattattc agcaagagaa caaaactgtg ccatcattga tggtactagc
     2281 ggtcattttt ggcttaagtt ttgccactcg ctttctccaa tttgtgcaaa ttgatacaga
     2341 aaaacaaccg cttgtagtga gtttagtaca agggaatatt gagcaaaaaa tgaagtggga
     2401 tccgcaacat ttcaaccaca gcgtacgaac ctacgaacag ctcattctgc ccctattagg
     2461 taacagtgaa ctgattattt tgcctgaatc cgccattccg gcacttgaaa atcaaatcag
     2521 cccgctgctt ggctcgctcg atcacttagc tcgccaacac ggaagtgaaa ttattatcgg
     2581 cacactctat caaaatgagc aagaggaact gtttaacagt agcgtgctat taggcgaaaa
     2641 agagagggaa tatgccctgc acagcaaccc tcgctacaac aaacatcacc ttgtgccgtt
     2701 tggcgaatat gtgccgtttg gctcggtgct ggattggatg cgggaagtgt ttattctacc
     2761 aattaatctt tcacaaggca gtttcgttca accatcattc ttggcgaaaa acaaccgatt
     2821 taatatggcg atttgctatg aaattatttt tggtgatcag ttacaacaaa accaaaaaag
     2881 ccaaaattca gattatttat taactatcac caatgatgcg tggtttggtt cttccatcgg
     2941 gccttggcaa catttccaaa tggcaagaat gcgagcgtta gaacttggca aaccattgct
     3001 tcgagctgcg aataccggta ttaccgccat tgttgatgcc aacggaaaag taattgaaca
     3061 actaccgcaa tttactgctg atgcactaac tgccaccatt gtgccaacca aaggcgatac
     3121 gccgtttaaa caattaggtc actggttaat ttacagtttc agcctatttt gtgtcattgc
     3181 cagcctgtta ctacgcagaa aataaaaaaa tccccttagg ctcttgggtc taaggggatt
     3241 ttatcgctga ttacgctaag gcttttaata ggttcgccat ttcaatcgct accattgcac
     3301 actcttcgcc tttattaccg gctttagtgc ctgaacgctc aatcgcttgc tctaaatttt
     3361 ccaccgttaa aataccgtta ataatcggca cgccggtttc taaagcagaa gagctgatgc
     3421 cttttgctga ctcgttcact accacatcat agtggctggt tgctccacgc acgaccgcac
     3481 ctaagcagat aatcgcatca taacgaccgc tttgtgccat ttttttcgct gcaattgaga
     3541 tttctaatgc acccggcacc catacggtgt cgatattttt ttcttctacg ccgtgacgca
     3601 ataatttatc ggttgcaccg tttaacagtt gatcgataaa aatcttgtgc cagttagttg
     3661 ccacaatacc gaattttaaa ccagttgcga tgtagtttcc ttgaaatgtt gccattgttt
     3721 gttttccttc tttaaaataa atttgatact caataaccct ctccccttgt gggagaggga
     3781 tagatttttc ttcgttcaga agaaaaatca gggagagggg aaattgcaaa ctttttctaa
     3841 aaaatatacc ctctctctga aaaatacttc tgaacgaagt atttttctgt ctctctccca
     3901 caaggggaga gagtattgga ttagaaatta aacatatgtc gcattttgtg ctgtttaact
     3961 ttaaggtatt ccaaatcgtg ttcgtttggc tcaaccataa tcggctcacg agccactacg
     4021 tttaaacctt gctcaatgag tccttcaatt tttgccggat tattggttaa caaacgaatc
     4081 gattttacgc ctaatttttc aaacatttga gcggcgatat aatattcacg ctcatcatct
     4141 ttgaaaccta atgccaggtt ggcttcaacc gtgtccattc ctctatcttg caattcgtaa
     4201 gcacgcagtt tattaattaa accgatgcca cgtccttctt ggcgtaaata aagcaccacg
     4261 cctctgccct cttgctccac ttggttcatt gccgctgcga actgctgacc acaatcgcaa
     4321 cgttgtgagc caaaggcatc gccggttaaa cattcggaat ggatacggca taatacattt
     4381 tcgccatcac caatatcacc tttaactaaa gcgacgtgtt ctttgcctga aatagtttca
     4441 acgaaactgt gtgctttgaa ctcaccgtat ttggtcggca tttttaccac cgaaaccaca
     4501 ttcaccaagc tgtcgtgttt tctacgatac tcttgtaact gctgaatagt aataaacggc
     4561 atattgtgtg cttgtgcaaa tgcctgtaat tccggcatct gcatcatcgt gccgtcttct
     4621 gccataattt cacagcaaag ccctgccggt ttcaagcccg ccaaacgagc taaatccaca
     4681 gttgcttcgg tatgaccgtt acgcactaat acgcctccgt cttttgccac taatgggaaa
     4741 acatgccccg gacgtctaaa atcttccggc ttagcatttt catcaagcat tttcaggcaa
     4801 gttaatgaac gctcaaaagc ggaaataccg gtgccggttt gaatatggtc aacagaaacg
     4861 gtaaatgcgg tttgatgatt atcttcgttc tctagcacca tcgggtgaaa atttaatttt
     4921 ttggcaattt ctgtcgaaat cggcgtacaa atcaaccctt taccatattt cgccataaag
     4981 ttgatatttt ccggtgtcgc aaattccgct gcacaaatga agtccccttc attttcacgg
     5041 tcggcatcat cgctgactaa aataattttc ccttgtgcaa ttgcttgaat tgcttcttct
     5101 accgttgaaa acttaaacat tatttgtcct ttatttggtt aaaaccctgc atttttaaga
     5161 aagtccgcag taattttact ttgcggattt tcaggctctt ttttcaataa aaactgttcg
     5221 atatatttgc cgacaatgtc attttctaaa ttcaccaaac taccgatttt tttcgtaccg
     5281 aggttggttt ccttaatggt atgcggaata atcgataccc ggaaagattc cgcatcaaca
     5341 tccaccaccg ttaaactaat gccatcaata gtaattgagc ctttttcaat gatgtaacgc
     5401 attaacttag gttcggcttt aatgcgatac caagttgaat tatctgctgg tgtaatctct
     5461 gcaaccacac ccgtgccatc aatatgacct gacacaatat gcccaccaaa acgcccgttt
     5521 gccgccattg cccgctcaag attaaccgag ctgttgatac ttaattcgcc aagtgatgta
     5581 cgttttaagg tttctgacat aacatcagca gtaaattgct cctgcgtaaa tgaagtcacg
     5641 gttaaacaca cgccatttac cgcaatgcta tcccctaaat gtacatcggt cagaatcttt
     5701 tttgctttaa ccgttaccac cacaaactcg ccttgtttct taatttgggc tatctggcca
     5761 acttcttcga taatgcctgt gaacatttaa atttttctcc gatttacttc caatttagtc
     5821 tgagaactgc ttatcttact attcccctct cccgtttacg ggagagggaa gattacactc
     5881 attttcccct ctctctgcat aaaatttctg aacgaaattt tatgctgtct ctctcccgta
     5941 aacgggcgag agtaaggaaa tttcaattta attacgaagc aagtccactt cataatccaa
     6001 caaaatatct tctccgagca attcggtgga ttttagacgt aatttaaccg cttgtgctaa
     6061 ctcgccaatc cccttgccac caatcggggt ttttgccgct ttgccgccaa tcagtttcgg
     6121 ggcaatatag caatgtactc gattgactaa cccctgttcc agcacgctga aattgagttg
     6181 cgaaccacct tcaatcaata agctgtcgat cccgagttcg cccagctttt gcaaaagatc
     6241 ggctaaatcc acccgcttgt cacgttcgcc tgtcactaaa atttcaacac cgcaagcggt
     6301 atattctgcc tgcttttttg catcattatt gagcgtagca ataatggtgc gatattggcg
     6361 tgcagtttgg actagctggc tatccaatgg ggtacgcagt tggctgtcac atacaatccg
     6421 caccggctgt ttagcatttt ccattcggct gttgagcatt gggtcatcgg caagcaccgt
     6481 ttccaccccg accataatcg cactatattc gtggcggctt ttctgtacat tagctcgggc
     6541 agcctcgccg gtgatccatt tcgaattccc cgtataagtc gcaattttac catctgccgt
     6601 catcccatat ttcagcaaca cataagggcg tttggtttga atgtaatgga agaaaatcgg
     6661 gttcagcgca tcgcactccg ttcgcataaa gtcttccaca acctcaatgc cggcggctct
     6721 aagctgtgcc gctcctttgc cagaaacaag tgggttcgga tctcgagagc cgataaacac
     6781 ttttttgatc ccacgttcaa tcagcagttc ggaacaaggc ggggttcgcc cgtggtggca
     6841 acaaggctct aaggttacat aagcggttgc ccccgtcaga tcttcctcgc agtttaaaat
     6901 cgcattgcgt tcagcgtgcc aactaccggc tttttggtga tacccttcgg cgataatgtt
     6961 gccgtttttc acgatcacac agccaactag cg
//
LOCUS       DANKBV010000122         6963 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.122, whole genome shotgun sequence.
ACCESSION   DANKBV010000122 DANKBV010000000
VERSION     DANKBV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.122"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     repeat_region   34..199
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=34..56
                     /rpt_unit_seq="gcagccacctacgcgtggctgtg"
     gene            449..1924
                     /gene="rng"
                     /locus_tag="PWY43_001991"
     CDS             449..1924
                     /gene="rng"
                     /locus_tag="PWY43_001991"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDL2136846.1"
                     /translation="MSSTELLVNVTPSETRVALVENGILKELHIEREAKRGIVGNIYK
                     GRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTECVDENEKKQFLVKDISELVREGQ
                     DIVVQVVKDPIGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKSLVE
                     PYCDELGGFIVRTAAEEATEESLKQDVEFLKRLWRKVLERKGKYSAKSMLYGELALAQ
                     RVLRDFIGTHINSVRIDSKLTYEQVREFLAEFMPELTDCVTLYTGQQTLFDAYGVEKA
                     IQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHTIFNTNIEAIQAIAH
                     QLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRK
                     RTRESLERILCCDCQACQGRGTVKTVETVCYEIMREIVRVHHLFKTENIHVYAAKDVA
                     EYLINEESHALLAELQAFIGKKIEIKLEPYYHQEQFDVVVM"
     gene            2078..3580
                     /gene="lysS"
                     /locus_tag="PWY43_001992"
     CDS             2078..3580
                     /gene="lysS"
                     /locus_tag="PWY43_001992"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL2136847.1"
                     /translation="MSEVENQELDLNGEMLARREKLNKIREQGNAFPNTFRRDALAKD
                     LHAQYDAVEGETLKEQNIQVKVAGRIMLKRVMGKASFFTIQDVSGAIQLYVARDNLSD
                     SVYEEKVSLWDLGDIVGVEGALFKTKTGELTVRCSEVQLLTKSLRPLPDKHHGLTDLE
                     TRYRQRYLDLISNEESRRTFMIRSQVVSGIRKFFLERDFIEVETPMLQVIPGGAAAKP
                     FITHHNALDVDMYLRIAPELYLKRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEY
                     YQAYADYHDLMDNTEELLRQLALDILGTTDVPYGDYVFDFGKPFERITMHDAIVKYGN
                     GIQREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAE
                     ISPLARRNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEA
                     MFKDEDFIVALEHGLPPTAGEGLGIDRLAMIFANAPSIRDVILFPAMRQK"
     gene            complement(3658..4731)
                     /locus_tag="PWY43_001993"
     CDS             complement(3658..4731)
                     /locus_tag="PWY43_001993"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HDL2136848.1"
                     /translation="MAQQITLFEDVPLPLTLGGELSPITVAYQTYGRLNAEKSNAVLL
                     CHALTGDAKPYYDSPTEKGWWQDFIGKGLAFDTDKYFFICSNVLGGCKGTTGPASINP
                     KTNKPYGSLFPIITVQDIVNVQKALLDYLEIPQLHAVVGGSFGGMQATQWGISYPDKV
                     RNIINLCSSLTLSAEAIGFNHVMRQAIINDPHFNGGNYYDGIAPDNGLKIARMLGMLT
                     YRTDIQLAKAFGRETKQQGQIWGDHFQVESYLSYQGTKFLGRFDANTYLRLLRALDLY
                     DPALGYENEETALKRIKANYTFVAVTSDQLFKQVDVYKSKQKLEQAGVNLEFHEFHSD
                     FGHDAFLVDYDFFEPMIKAGLAK"
     gene            4999..5682
                     /locus_tag="PWY43_001994"
     CDS             4999..5682
                     /locus_tag="PWY43_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2136849.1"
                     /translation="MNQTRDRLEKLPLFHKMQQFIIKALKIIAIFPLLGIAILLLVDS
                     LTSYIVRDKIYTDSKQIPYREYAVVLGTAKFYSKDVINQYYKYRLEAAKQLVQEGKVN
                     QLLLSGDNKTPYYNEPKTMTSDLLKMGIATSKIKQDYAGYTTFDSIIRAKKVYKLPPF
                     TIISQKFHCERALFIAKFRDIDAICYAATYPEGAYQVRMREILARSAMVFSLLIGKEP
                     ESLEDIRSK"
     gene            complement(5719..6558)
                     /gene="purU"
                     /locus_tag="PWY43_001995"
     CDS             complement(5719..6558)
                     /gene="purU"
                     /locus_tag="PWY43_001995"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDL2136850.1"
                     /translation="MLQQHKILLTECPDDTGLVAKITNICYKHQLNIIKNSEFVENET
                     KRFFMRTELNGIFNDETLLTDLKFTLPEGSSYKLLPKQRKRIVILVTKEAHCLGDLLM
                     KNYYGGLDVEIAAVIGNHETLKSLVERFDIPFHLVSHENLTRVEHDKLLAEKIDEYSP
                     DYIVLAKYMRVLNPEFVARYPNRVVNIHHSFLPAFIGAKPYHRAYERGVKIIGATAHF
                     VNDELDEGPIIMQNVINVDHTYTAEAMMRAGRDVEKTVLSQALELVLADKVFVYKNKT
                     IIL"
     gene            complement(6628..>6963)
                     /locus_tag="PWY43_001996"
     CDS             complement(6628..>6963)
                     /locus_tag="PWY43_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDL2136851.1"
                     /translation="KSISEKLDIIIAEKEEEMKQEELEKLKQLESLNKYREMLKQDGL
                     SIDELAELLADKVIKTRKARKALSPRPAKYKFVDANGNEKTWTGQGRTPKALQDALDS
                     GKKLEDFAI"
BASE COUNT         2116 a         1194 c         1469 g         2184 t
ORIGIN      
        1 cttacatcta gacagacgag caagtgctga aacgcagcca cctacgcgtg gctgtgaatt
       61 gaaacactga catcggcgta aatgcgtgga agattttcga gcagccacct acgcgtggct
      121 gtgaattgaa actccgtgtt tgtggttatg ttgatgtaca ggcaattgca gccacctacg
      181 cgtggctgtg tattgaaacg gtgtacccac aaatcctatg ttttcgccat tggctgctac
      241 cttcgcatca atatatattg aaatatcaat ggcaaatttg tatgttgtta attagactga
      301 ttgcatctgc tatgtatcat agtaatgacg aaaatattct attttgcaaa aaataatcag
      361 aaattacccg cttgttcgaa ctgagttttt gttttctaag atgtgaaatg atacacttta
      421 gatagtttta aaatatagag acagtaagat gagtagtacg gaattattag tgaatgttac
      481 cccaagcgaa acaagggtag cgttggtaga aaacggaata ttgaaagaat tacatattga
      541 gcgggaagct aaacgcggga ttgttggtaa tatttataag ggaagggtga caagggtatt
      601 accaggtatg caatctgctt ttgtggatat cggcttggag aaggctgctt ttttgcacgc
      661 ttctgacatc gtttcgcata cggaatgtgt tgatgaaaat gaaaagaagc aatttctggt
      721 taaagatatt tctgaattgg tgcgtgaagg gcaagatata gttgtacaag tagtgaaaga
      781 tccaattggt actaaaggag ctaggctcac tactgatatt acgttaccat ctcgttattt
      841 agtgtttatg ccagaaaata gccatgtggg agtatctcag cggattgaaa gtgaagaaga
      901 gcgtgctcgt ttaaaaagct tggttgagcc ttattgtgat gaacttgggg gctttattgt
      961 acgtaccgct gcagaagagg caactgaaga aagtttaaag caagatgttg aatttttaaa
     1021 acggttgtgg cgtaaagtat tagaacgaaa agggaaatat tccgcaaaat caatgcttta
     1081 tggagagctt gcgcttgctc aaagggtact gcgtgatttt attggtacac atatcaattc
     1141 ggttagaatc gattcaaagc taacttatga gcaagtgcga gaatttttag ctgagtttat
     1201 gccagaatta acggattgtg tcaccctcta taccggtcag caaactttat ttgatgccta
     1261 tggtgtagaa aaggcaatcc aaaaggcatt ggataaacgg gttgatttaa aatcgggggg
     1321 ctatttaatt attgagcaaa ctgaggcgat gacgactatt gatattaata ctggtgcttt
     1381 tgtcggacat cgtaaccttg cacatacgat tttcaacaca aatattgaag caatacaggc
     1441 aattgcacac cagctacagt tgcgtaattt aggtggtatt atcattattg attttattga
     1501 tatgcaagag gaagaacatc gtgagcgcgt tttacaatcg ttacaagagg cattaaaagg
     1561 cgatcgtgtt aaaaccaatg tgaatggctt tactcagctt ggcttggtgg agatgaccag
     1621 aaaacgtaca agggagagtc tagagcgaat tctttgttgc gattgccaag cttgccaagg
     1681 gcgtggcaca gtgaaaactg tagagacggt ttgctatgaa attatgcgtg aaattgtgcg
     1741 tgtacatcat ttgtttaaga cagaaaatat tcacgtttat gcagctaaag atgttgctga
     1801 atatttaatt aacgaggaaa gccacgcatt attggctgag cttcaggcgt ttatcggtaa
     1861 aaaaattgag attaaacttg agccatatta tcatcaagag cagtttgatg tggtcgtgat
     1921 gtaatctatt acttggttga ggctaaatag ttagcctcaa tatcgttagt tacaaaaaat
     1981 ttagaaaatc tgaccgcttg tttcctatcc aaattcctca aaataacgta taatcgtcca
     2041 gttttattta tccttttttg aaatctaagg taattcaatg tctgaagtag aaaatcaaga
     2101 attagatcta aacggtgaaa tgttagcccg ccgtgaaaaa ttaaacaaaa tccgtgagca
     2161 gggtaacgca tttccaaata cattccgtcg tgatgcgtta gccaaagatc tgcacgctca
     2221 atatgatgca gtggaaggtg aaaccttaaa agaacagaat attcaagtga aagttgccgg
     2281 tcgtattatg ttaaaacgag taatgggtaa ggcatctttc tttaccattc aagatgtgag
     2341 cggtgcgatt cagctttatg tagctcgtga taacttaagt gacagcgttt acgaagaaaa
     2401 agtcagcctt tgggatttag gcgacattgt gggcgtcgaa ggtgcgttat ttaaaaccaa
     2461 aaccggtgaa ttaacggttc gttgctctga agttcaactt ttaaccaagt ctttacgccc
     2521 gttgccggat aaacatcacg gtttaacgga tttagaaact cgttatcgtc agcgttattt
     2581 agacttgatt tctaatgaag aatctcgccg tacttttatg atccgctcgc aagtagtttc
     2641 aggcattcgc aaattcttct tagaaagaga ctttattgaa gttgaaacgc caatgttgca
     2701 agtgatcccg ggcggtgcgg cagcgaaacc gtttatcact catcataatg cgttagatgt
     2761 ggatatgtat ctgcgtattg ctccagagct ttacttaaaa cgtttagttg tgggtggttt
     2821 tgagcgagta tttgagctta accgtaactt ccgtaacgaa ggggtttcag tacgccacaa
     2881 tcctgaattt acaatgattg agtactacca agcctatgcg gattatcacg atttaatgga
     2941 taataccgaa gaactgttac gccaattagc cttagatatt cttggcacaa ctgacgtacc
     3001 ttatggtgat tatgtattcg atttcggtaa accgtttgag cgtattacga tgcacgatgc
     3061 gattgtgaaa tatggtaacg gcattcaacg tgaagattta gacagcttcg agaaaagtgt
     3121 ggaaattgcg aaaggcttag gcattgaaat tcaaaaatct tggggtttag gctcggtagt
     3181 caatgcgatt tttgaagaag tggcagaaca tcaattaatt cagccaactt tcttgatggc
     3241 tcacccggca gaaatttcac cattagctcg tcgtaatgat gaaaacccag aggtaactga
     3301 tcgttttgag ttatttatcg gtggtcgtga aattggtaat ggtttctcag aattaaacga
     3361 tgcagaagac caagcagagc gttttgatgc tcaagttgcg gcgaaagatg ccggtgatga
     3421 tgaagcaatg tttaaagatg aagactttat cgtggcattg gaacacggtc tgccgccaac
     3481 agcgggtgaa ggtttaggaa ttgaccgctt agcaatgatt ttcgctaacg ccccatcaat
     3541 ccgtgatgta attttattcc cagcgatgcg tcagaagtaa tcagaaatag aacaaaagca
     3601 cacttcggtg tgcttttttg tattacaagc ggtcaaattt ataataaaaa atgcaattta
     3661 ttttgctaat ccggctttaa tcatcggttc aaaaaagtca taatcaacca aaaaggcatc
     3721 gtgtccgaaa tcggagtgaa actcgtggaa ctccaaattt acgcctgcct gttctaattt
     3781 ttgtttgctc ttatacacgt caacttgctt gaatagttgg tcgcttgtta ccgctacaaa
     3841 agtgtagttg gctttaatac gttttagggc ggtttcttca ttttcatagc caagagccgg
     3901 gtcgtataaa tctaatgctc ggagtaaacg gaggtaagtg ttggcatcga aacgacctaa
     3961 aaatttcgtg ccttgatagc ttaaatagga ttccacttgg aaatgatcgc cccaaatttg
     4021 tccttgctgt ttggtttctc gtccaaaggc tttagcaagt tgaatatcag tacggtaagt
     4081 cagcataccg agcatacggg cgatttttaa gccgttatca ggtgcaatgc catcataata
     4141 gttcccaccg ttaaagtgtg gatcgttaat aatagcttgg cgcataacgt ggttaaaacc
     4201 gatagcctcg gcacttaggg taagtgacga acaaaggttt ataatgttgc gaactttatc
     4261 agggtaacta ataccccact gtgttgcttg cattccgccg aaagatcccc ctaccactgc
     4321 gtgaagttga gggatttcta aatagtccaa taatgctttt tgtacattaa caatatcttg
     4381 cactgtaata attggaaaca aactaccgta aggtttattg gttttagggt taatcgaagc
     4441 tgggccggtt gtgcctttac aaccgcctaa tacattactg caaataaaga aatatttatc
     4501 ggtatcaaac gcaagccctt tgccgatgaa atcttgccac cagcctttct cggttggtga
     4561 gtcatagtaa ggcttagcat caccggtaag ggcgtggcaa agcaagaccg cattgctttt
     4621 ctctgcattt aatctgccgt aagtctggta agccactgtg ataggggaca gttcgcctcc
     4681 taatgtgagt ggtaaaggta cgtcctcaaa aagggtaatc tgctgagcca taaaaatcct
     4741 gtttgcaaaa aattctgctt ttttgaccgc ttgttgtaat gaccgttaaa ttagcggtgt
     4801 tcatttcaat tgtcaagaat ttttagacgt ctaaacgtct agatttttaa acggctaaaa
     4861 tcaaaatttt attgctgaga agatatttat tacttaacaa ataacatgaa atagaataaa
     4921 tttattgaac gattagacta ttttgtttgt ctctaattta cccatcttaa atagggtagc
     4981 aaaagtaaag gatctcattt gaaccaaacg cgtgataggt tagaaaaatt gccacttttc
     5041 cataaaatgc agcagtttat tattaaggca ttgaaaataa ttgcaatttt ccccttattg
     5101 ggaatagcta tattattatt ggttgattca cttacaagtt atattgtgag ggataaaatt
     5161 tatacagata gtaaacagat tccttaccgg gagtatgcag ttgtactcgg tacggcaaaa
     5221 ttttactcta aagatgtgat taaccaatat tataaatatc gtttggaagc cgctaagcaa
     5281 ttagtccaag aaggaaaggt caatcagctt ttgttaagtg gagataataa aacgccgtac
     5341 tataatgagc caaaaacaat gacaagtgat ctacttaaaa tggggattgc gacttcaaaa
     5401 attaagcagg attatgcggg gtataccact tttgattcaa taattagggc taaaaaagta
     5461 tataaattac cgccatttac gattatctcc caaaaattcc actgtgaaag ggcattgttt
     5521 attgcaaaat ttagggatat tgatgcaatt tgttatgccg ctacatatcc agagggggct
     5581 tatcaggtac gaatgcggga aattttagcc agatcggcaa tggtttttag tttgttgata
     5641 ggtaaagagc ctgaatcatt ggaagatatc cgttctaaat gagatagccg gtcagataat
     5701 gttgaccggc tattttgctt ataaaataat ggttttgttt ttatatacaa atactttgtc
     5761 agcaagcact aattctaagg cttggctaag tacggttttc tcgacatcac gtccggcacg
     5821 catcatagcc tctgcggtgt aggtgtgatc aacattaatc acattttgca taataatcgg
     5881 gccttcatct aattcatcat tcacaaaatg ggcggttgca ccgataattt tcacaccacg
     5941 ttcgtatgca cgatgataag gttttgcacc aatgaaagcg ggtaagaacg agtggtgaat
     6001 attgacaact cggtttggat aacgagcgac aaattccgga ttcagcaccc gcatatattt
     6061 agccaacaca atataatcag gtgaatattc atcaattttt tccgctaata atttatcgtg
     6121 ttccactcgg gttaaatttt cgtggctgac taaatggaat ggaatatcga aacgttcaac
     6181 taaacttttt aatgtttcgt ggtttccaat cacggcagca atttctacat ctaatccacc
     6241 atagtaattt ttcatcagta agtcacctaa acaatgtgct tctttggtaa ctaaaataac
     6301 gatacgtttt ctttgtttag ggagtaattt atagcttgag ccttcaggta aggtaaattt
     6361 taaatcagtg agtaaggttt catcattaaa aattccgttt aattcggtac gcataaaaaa
     6421 acgtttagtt tcattctcaa caaattcaga atttttaata atatttaatt ggtgcttata
     6481 gcaaatattg gtaattttag cgacaagtcc tgtatcatca ggacattcgg ttaataatat
     6541 tttgtgttgt tgtagcattt catttcctat tataaaaata agagaactca attgagttct
     6601 ctttaaacta aaaatagtta tagccaatta aatagcaaaa tcttctaatt ttttaccact
     6661 atctaaagca tcttgtaatg cctttggtgt tctgccttga cctgtccaag ttttctcatt
     6721 gccgttagca tcaacaaatt tatatttagc cgggcgaggt gatagtgctt tacgtgcttt
     6781 gcgggttttg attactttat ctgctaataa ttcagctaat tcatcaatag ataacccatc
     6841 ttgttttagc atttctctat atttatttaa gctttctaat tgttttagtt tttctaattc
     6901 ttcctgtttc atttcttctt ctttttcagc aataattata tctaatttct cagaaattga
     6961 ttt
//
LOCUS       DANKBV010000123         6764 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.123, whole genome shotgun sequence.
ACCESSION   DANKBV010000123 DANKBV010000000
VERSION     DANKBV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.123"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(112..861)
                     /gene="moeB"
                     /locus_tag="PWY43_001997"
     CDS             complement(112..861)
                     /gene="moeB"
                     /locus_tag="PWY43_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDL2136852.1"
                     /translation="MELTDHEMLRYNRQIILKSVDFDGQEKLKASRVLIVGLGGLGCA
                     ASQYLASAGVGHLTLVDFDLVSLSNLQRQILHTDATIGQPKVESAKARLAAINPNIQI
                     ETINQRCSDEQLAKLIANVDLVLDCTDNVTVRNQLNLQCFKQKRPLVSGSAIRFEGQV
                     SVFCYQEQEPCYECLSRLFGDATLSCVEAGVIAPIVGIIGSIQALEAIKVLLNIGRTL
                     SGKLLMIDGLHFSVREMALPKQNNCPVCTSY"
     gene            complement(960..2189)
                     /gene="moeA"
                     /locus_tag="PWY43_001998"
     CDS             complement(960..2189)
                     /gene="moeA"
                     /locus_tag="PWY43_001998"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDL2136853.1"
                     /translation="MALLALSDALDNMLARLSTPKLVEQLPLNECANRVLAEDIFSPI
                     NVPNFDNSAMDGYAVRISDLAQNLTLNVAGKAFAGNPFNGEIKAGECVRIMTGALVPQ
                     GVDAVVMQEETTLNSDGSVTFNHIPKLGSNIRRIGEDVKQGALVLKKGSLLNVASLPL
                     LASLGIASVPVFVKLKVAILSTGDELTSVGEPLSEGKIYDTNRFAVRLMLEKLNCEIL
                     DFGILPDDPILFEQTFRQAQQQADVLITSGGVSVGEADFTKDVLEKLGEIGFWKIAMK
                     PGKPFAFGKLEQAWFFGLPGNPVSALVTFYQLAQPALAKLSGLSTDKIAKLTQNLTAC
                     SAGKLKKAVGRQDFQRGYYYANEKGELEVRAVGTQGSHIFSAFNESNCFIVLEAERGN
                     VDAGEKVTIQPFNSLMY"
     gene            complement(2211..2828)
                     /locus_tag="PWY43_001999"
     CDS             complement(2211..2828)
                     /locus_tag="PWY43_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HDL2136854.1"
                     /translation="MFDSLAVQFVVLWAVIDPIGSVPVYLAKTIGLPIEARRKIALNA
                     VLIAAGILIFFLIGGQVLLESMQIPLPAFQAAGGLVLLLFALTMIFGESKPEQEMRIS
                     SNLNELAVYPLAVPSIASPGAMMAIVLLTDNHRFHIGDQIITAGIMLLVLFITYLLLL
                     AANRIQHFIGNAGAAIISRVMGLILAAIAVNNLLIGVRDFFVQIS"
     gene            complement(2896..4107)
                     /locus_tag="PWY43_002000"
     CDS             complement(2896..4107)
                     /locus_tag="PWY43_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2136855.1"
                     /translation="MISRQMQSLLKRLVAQFPAVLVTGARQVGKSTLLQHIGEGYQYI
                     TFDDPMLLELAKQKSRLFFLNHQGKLILDEVQYVPEIFALLKLEIDKKQQNGLFLLSG
                     SQAFELMQNVSETLAGRIAILKLQGLSLREILGVKFDQPFLPTTDYLLAREQELKSPE
                     NIWQLIHKGDMPRLYAQEVDWEIYYRSYVATYIERDVRQLTNIASTTDFTRFMVSIAS
                     RSGELLNYSNIAQEVGVSNETIKRWVAILQTSGIIYLLQPYHNNHLKRAIKTPKVYFL
                     DTGLMAYLTKWLTPETIAQGAKSWQFFETFVVSQIIKSFHNQGIEPPIYFYRDTNQKE
                     IDLIIEYDRTLYPIEIKVTASPTKKMAKSFDLLNSVSSEWKLGQGAIINQYPTKLYLS
                     ENLVALPVSYL"
     gene            4304..6235
                     /locus_tag="PWY43_002001"
     CDS             4304..6235
                     /locus_tag="PWY43_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2136856.1"
                     /translation="MIFFTDLILKRGHSVLIDNSSVTIHTGQKVGLVGKNGCGKSSLF
                     ALLKNELQPEGGDVSLPKNWAISWVNQETPALAISALDYVIQGDREYTHLMAELDIAN
                     EKNDGNRIAIIHTQLDTIDAWTIQSRAATLLNGLGFSTEQLQLPVKSFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLERWLSNYRGTLLLISHDRDFLDPIIDRVIH
                     IEHQKLNEYTGNYTSFEIQRATKIAQQNAAYQQQQRKVAHLQSFIDRFKAKATKAKQA
                     QSRIKALEKMELIAPAYADSPFEFEFRPPLALPSPLLMMEKVSAGYAEKTVLESVKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELPPQNGSIQLAKGVQLGYFAQHQVDTLRFDE
                     SPLWHLQKLAPEKTEQELRNYLGGFDFKGDKVVQAVKSFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLEMRQALVDALTYYEGSLVVVSHDRHLLRSTVNEFYLVHDRKVEKF
                     KGDLDDYQKWLNEQNALANEPKSVLAKDSANSTACSSTNENSAAQRKEQKRLEAERRQ
                     QTAPLRKKITQLEKELEKATEKLNKLEAMLTESDIYEAENKAKLTETLAKQLEAKRLV
                     EEIEMQWLEAQEEFETMLR"
     gene            6514..>6764
                     /locus_tag="PWY43_002002"
     CDS             6514..>6764
                     /locus_tag="PWY43_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2136857.1"
                     /translation="MKNLRLSIHSSEHIWLRELFLKRRLELGLTQRTLGEKMGVLYSF
                     IGKVETGDRRLDIFEFIAYCKGLDLDPLSVLQEIEQQFP"
BASE COUNT         2104 a         1495 c         1301 g         1864 t
ORIGIN      
        1 acggtgtaaa taataggcta aaacgtttta gccgtcaaat aaaaaaaata aactgtcgtt
       61 cattttttac atttcttgac attttttcgc caataagtat gagaaaaata cttaataaga
      121 ggtacaaacc ggacagttat tttgcttagg taatgccatt tctctgacag aaaaatgcaa
      181 gccgtcaatc attaacaatt ttccggaaag agttctgccg atattaagta gcactttaat
      241 tgcttccaac gcttgaatac tgcctatgat gccaacaatc ggagcaatga cccccgcttc
      301 aacacaactt aaagtcgcat caccaaacaa cctgcttaaa cattcatagc acggttcttg
      361 ttcttgataa caaaatacac tcacctgccc ttcaaatcga attgccgaac cggaaacaag
      421 cggtcgtttt tgcttaaaac attgcaaatt taactgatta cgcacagtaa cgttatcggt
      481 acaatccagc actaaatcta catttgcaat cagcttggct aactgctcat cggaacatcg
      541 ctgattgata gtttcaatct gaatgttcgg attaatcgcc gctaatcgag ctttggcaga
      601 ttccaccttt ggctgcccga tagtcgcatc ggtatgtaaa atttggcgtt gcagattaga
      661 aagtgagacc agatcaaaat ccaccaaagt caaatgccca acgccggcag aagctaagta
      721 ttgcgatgcc gcacagccca aaccacctaa accgacaatc aacactcggc tggcttttaa
      781 cttttcctgc ccgtcaaaat ccacgctttt taggataatc tggcgattat aacgtagcat
      841 ttcgtgatcg gtaagttcca tttttacacc taaagagtaa acgatggcgc aagcgtccac
      901 gcttgtgcct atatttttat tgctagcaca agcgtggacg cttgcgctat cttaattcat
      961 taatacatca gcgaattaaa cggctgaata gtgacttttt ccccagcatc aacattgcct
     1021 cgttctgctt ctagcacaat aaaacagtta ctttcattaa aagcgctgaa aatatgtgag
     1081 ccttgcgtgc caactgcacg aacttctaac tcaccttttt cattggcata gtaatagccc
     1141 cgttggaaat cttggcgacc gaccgctttt ttcaattttc cggcactgca agcggttaga
     1201 ttttgggtta attttgcaat tttatctgtc gataaacccg aaagtttggc taacgccggt
     1261 tgcgctaatt ggtagaaagt cactaatgca gagacagggt tgcccggcag cccaaaaaac
     1321 caagcctgtt ctaatttacc gaatgcaaaa ggcttgcccg gcttcatcgc aattttccag
     1381 aaaccgattt cgcctaattt ttccagtaca tctttggtaa aatctgcctc tcccacagat
     1441 acgccgccac tggtaatcaa cacatcggct tgctgttggg cttgcctgaa agtttgctca
     1501 aacagaattg ggtcatcagg caaaatccca aaatctaaaa tctcgcagtt gagtttttcc
     1561 agcattaaac gcaccgcaaa acggttggtg tcgtaaattt ttccctcact caaaggctcg
     1621 ccaactgagg ttaattcatc gccggttgaa agaattgcca cttttaattt tacaaaaaca
     1681 ggcacagaag caataccgag tgaagctagc aacggtaaac tggctacatt caataatgag
     1741 ccttttttca agaccaatgc cccctgttta acatcttccc caatgcggcg aatgttactg
     1801 cctaattttg gaatatggtt aaaggttaca gagccatcgg aatttaacgt agtttcctct
     1861 tgcattacaa cggcatcaac gccctgtggc actaatgctc ccgtcataat ccgcacgcat
     1921 tcgcctgcct ttatttcgcc gttaaacggg ttaccggcaa aggctttacc agccacattc
     1981 aaggttaaat tttgtgctaa atctgaaata cgaaccgcgt agccgtccat cgccgaatta
     2041 tcaaaattcg gtacattaat cggggaaaaa atatcctctg ccaacactcg gttagcacac
     2101 tcatttaacg gaagttgttc caccaattta ggtgtcgata aacgagcaag catattatct
     2161 aacgcatcac tcaaagccaa aagtgccata atttcctacc tattcaacgt ttagctaatt
     2221 tgtacaaaga aatcccgtac gccaatcaac agattattca ccgcaatcgc agccagaatt
     2281 aaccccatca ctcggctgat aatcgcagcc cctgcattac caataaagtg ctgaatacgg
     2341 tttgccgcta atagcaacaa ataggtaata aagagtacca aaagcataat gccggcggtg
     2401 ataatttgat cgccgatatg aaaacggtga ttgtcggtta ataatacgat tgccatcatc
     2461 gccccgggag aagcaatcga cggtacagct agcggataaa ccgccaattc attcagatta
     2521 ctgctgatcc gcatttcttg ctcgggcttg ctttcgccaa aaatcatcgt taaagcaaat
     2581 aacaacagca ctaagccgcc tgccgcttgg aatgcaggta acggaatctg cattgattct
     2641 aacagcactt gcccaccgat aaggaagaaa atcaaaatac cggcggcaat caagaccgca
     2701 tttagtgcaa ttttacgccg tgcttcaatc ggcaaaccaa tggtttttgc caaataaaca
     2761 ggaacagaac caatcgggtc gatcaccgcc cataaaacca caaattgtac cgctaatgaa
     2821 tcaaacatat tctctcctgt tatcttgata atttgcaaaa ttttagcata aaacaaccgc
     2881 ttgcacggct ttttcttaca gatagctcac cggtaacgct accaaatttt cacttaaata
     2941 aagtttggtc ggatattggt taataatcgc tccctgccct aatttccatt cactagaaac
     3001 tgagtttagc aagtcaaaac tttttgccat ttttttggtt gggcttgccg tgaccttaat
     3061 ttctatcgga taaagcgtgc gatcatattc aataattaag tcgatctctt tctggttagt
     3121 atcacggtaa aaataaatcg gcggctcaat tccttgatta tgaaaggatt ttataatttg
     3181 gcttaccaca aaggtttcaa agaactgcca gctttttgcc ccttgagcaa tagtttcggg
     3241 ggtaagccat ttggttaaat acgccattaa cccggtgtct agaaaataca cttttggggt
     3301 cttaattgcc cgtttcaaat gattgttgtg gtatggctgg agcaaataga taatgccgga
     3361 agtctgcaaa atggcgaccc aacgtttaat cgtttcattc gatactccaa cttcttgagc
     3421 aatattactg taattgagta gctcaccact acgagaggca atagagacca taaaacgggt
     3481 aaaatccgtt gtgctggcaa tattggttaa ttggcgaaca tctcgctcaa tgtaggtcgc
     3541 tacataagag cgataataaa tctcccaatc cacctcttgt gcatataaac gaggcatatc
     3601 gcctttatgg ataagctgcc agatgttctc cggtgatttt aattcttgct cccgagctaa
     3661 caaatagtcg gttgtcggta aaaaaggttg atcgaacttc acccctaaaa tttcacgcaa
     3721 cgaaagccct tgtaatttta aaatagcaat acgaccggca agtgtttcac tcacattctg
     3781 cattaattca aaggcttgcg accccgaaag taaaaacaaa ccgttttgct gttttttatc
     3841 aatttctaac tttaataacg caaaaatttc aggcacatac tgtacttcgt ctaaaataag
     3901 cttgccttga tggtttaaaa aaaacaggcg agatttttgt ttagccagct ccagcaacat
     3961 tgggtcatca aaagtaatat attgatagcc ctcaccaata tgttgtaaca atgtcgattt
     4021 accaacctgt cttgcccctg tcactaaaac ggcaggaaat tgcgccacga gtcgttttaa
     4081 cagcgattgc atttgccgag aaatcatttt tcctccaaaa tagcctaaat caagtttaac
     4141 ctcatatata atgttgaatt aaacttaatt taaattattt tttaagttta attcaacaca
     4201 gaatattgaa ttaaactgaa tttcttattt tagttcacaa agcaatggct ttgctttttt
     4261 atgctaaaat acgcaaaatt tttaacaaaa tagaccgctt gcaatgatct tttttacaga
     4321 cttaatttta aaacgagggc attccgttct cattgataac agctccgtta ccattcacac
     4381 cgggcagaaa gtcggtttgg tgggaaaaaa cggctgtggc aaatcctctc tttttgcctt
     4441 actgaaaaat gaattacaac ctgaaggtgg cgatgtatcg ttgccgaaaa actgggcgat
     4501 ctcgtgggta aatcaagaaa cgcctgcgtt agctatttca gcgttagact atgtgattca
     4561 aggagatcgg gaatataccc atcttatggc tgagctagat atcgcaaatg aaaagaatga
     4621 cggcaaccgc attgcgatta tccatactca acttgatacc attgatgctt ggacaattca
     4681 atcccgtgcc gctaccctgc ttaacggctt ggggtttagc accgaacaac tgcaactgcc
     4741 ggtgaaatcc ttctccggtg gctggcgtat gcggttaaat ttggcacaag ctctgatttg
     4801 tcgttccgat ctgttattgc ttgacgagcc aaccaaccac ttagacttag atgcggttat
     4861 ttggttggag cgttggctga gtaactatcg tggcacttta ttgttaattt cgcacgaccg
     4921 tgattttctc gatccgatta tcgatcgggt gattcatatt gaacatcaaa agctcaatga
     4981 atacaccggc aattacacct cgtttgagat tcaacgtgcg accaaaatcg cccagcagaa
     5041 tgccgcttat cagcaacagc aacgtaaagt tgctcatttg cagagtttta tcgatcgctt
     5101 taaagccaaa gcgactaaag cgaaacaggc acaaagccga attaaagcgt tggaaaaaat
     5161 ggagcttatt gccccagcct atgctgattc gccgtttgaa tttgaatttc gcccaccgct
     5221 tgcactgcct agcccgttat taatgatgga aaaagtcagt gccggctatg cggaaaaaac
     5281 ggtgttggaa tcggtcaaac tgaatttggt tcccggctct cgtattggtt tactcgggag
     5341 aaacggtgca gggaaatcca cgctgattaa attattggcg ggcgaacttc cgccccaaaa
     5401 cggctcaatt caactggcaa aaggcgtgca actcggctat ttcgcccaac atcaggtgga
     5461 tacactacgt tttgatgaaa gcccactttg gcatttacaa aaactcgcac cggaaaaaac
     5521 cgagcaggaa ttacgcaatt atttaggcgg tttcgatttt aaaggcgata aagtggtaca
     5581 agctgtgaaa tcattctccg gcggagaaaa ggcccgttta gtgcttgcct taattgtatg
     5641 gcaacgcccg aatttactgc tattagatga gccaaccaac catttagatt tagaaatgcg
     5701 acaagccctg gttgatgcac tgacctatta cgaaggctcg ctagtggtgg tttcgcacga
     5761 ccgccattta ttgcgtagca ccgtgaatga attttattta gtgcacgacc gcaaagtgga
     5821 aaaatttaaa ggtgatttag acgattacca aaaatggctc aatgagcaaa acgccttagc
     5881 caacgagccg aaatccgtac ttgcaaaaga ttcggcaaat tcgaccgctt gctctagcac
     5941 caatgaaaat agtgcagctc aacgcaaaga gcaaaaacgc ttagaggcag agcgccgcca
     6001 acaaaccgcc ccattgcgga aaaaaattac tcaactggaa aaagagttag aaaaagcaac
     6061 cgaaaaatta aataaactcg aagcaatgct taccgaaagc gacatttatg aagccgaaaa
     6121 taaagcaaaa ctaactgaaa cacttgcgaa acagttagag gcaaaaaggc tggttgaaga
     6181 aattgaaatg caatggttgg aggctcagga ggagtttgag accatgttac gctagggttc
     6241 tacaagatag cgcaagcgtc cacgcttgtg ccatttaagg gaactataca cctcaaaaaa
     6301 acaatgatca ttaagctagc ttttttataa atttaaaaac ctatagatta aataaataat
     6361 gatattaata gtaaaatcac gagtgcaact acactgacga taataaaatc aggcatactt
     6421 attctcccta taaaatgtgg taatgtaact acaaattaat tatataatga acctaaaaaa
     6481 ggttcaacat tatttgtacc taatataggt gcaatgaaaa atcttagatt atcaatacat
     6541 tccagcgagc atatttggtt aagagaacta ttcttaaaac gccgtttgga actagggcta
     6601 acacaacgca cattaggaga aaaaatgggc gttctttact catttatcgg aaaagtagaa
     6661 acaggagatc gcagattaga tatttttgaa tttatcgctt attgcaaagg gttagattta
     6721 gatcctttat ctgtattgca agaaattgaa caacaatttc ctca
//
LOCUS       DANKBV010000124         6704 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.124, whole genome shotgun sequence.
ACCESSION   DANKBV010000124 DANKBV010000000
VERSION     DANKBV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.124"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..617
                     /gene="xseA"
                     /locus_tag="PWY43_002003"
     CDS             <1..617
                     /gene="xseA"
                     /locus_tag="PWY43_002003"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251679.1"
                     /note="bidirectionally degrades single-stranded DNA into
                     large acid-insoluble oligonucleotides; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2136858.1"
                     /translation="VWNHKFNRFQQLPLRLNAQHPSKQIQSQQQRFDYMEYRLEQAIY
                     ALVAAKKQVGKELNVRLIHQHPQKQIERQSQMLKQLELKLHWQMEQQLSKKQQKWKNI
                     TQRFERNPLPYQLYQQQTMVAKMSQRLAYSIEKIMLKEQRHFQELCTKLDGLSPLKIL
                     TRGYSITQTKDGKTLTSTENIERGEAIITQLKTGKIISQVVDFL"
     gene            930..1015
                     /locus_tag="PWY43_002004"
     tRNA            930..1015
                     /locus_tag="PWY43_002004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:964..966,aa:Leu,seq:caa)
     gene            1109..1933
                     /locus_tag="PWY43_002005"
     CDS             1109..1933
                     /locus_tag="PWY43_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2136859.1"
                     /translation="MKPLQNGTNKPFTITIASTKGGSAKSTNAANIGAFCAEHGLKTL
                     LIDTDTQPTLSSYYELAYQVPGGTYEFLHFKDVEPTHIISKTTIPNLDLIQSNDPSNK
                     ISPMLRDSPDGALRFSLLLQKIEGYDVIIIDTRGTRDITVDMSVLAADVLFCPILPHI
                     LSAKEFIRGTIGMYQDLETFTAFGFTLPPLKAMINCVDHTKDVKLVSDYLHTLFQNEF
                     DENKTLLEFSVPDRVAYREAATFSKPVYRQSQAEYETIRQLCALLMPQFAQSHFSE"
     gene            1945..3306
                     /gene="dnaB"
                     /locus_tag="PWY43_002006"
     CDS             1945..3306
                     /gene="dnaB"
                     /locus_tag="PWY43_002006"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2136860.1"
                     /translation="MSNPNKPTTHLYSAEAEAAVIGGLLLDNTLFDDVIGKINSADFY
                     FGVHQALFKGITDLIEVGKPADILTLDEYLKQKNLLKEIGGFAYLAEVSKNTPSAANV
                     GAYADVVLLHSKHRQLLKLGQFIVDQTQTAKTPEKLESVIDEVEKKMTEFSLSDNTKS
                     ATDLSDIFASMLSRMELSAKNGDPVTGTPTGIQGIDEATTGGQPGDLIVIGARPSMGK
                     TAFSQTIAYHTLEKFESAPVFYHSMEMPADQILQRFLAMRARVSLQDIRQADRLDDED
                     WGKLTMAMGHILEHWKNRLVIDDEGSLTPQKLRTKVRQNARKYGKPAAIFIDYIQLMQ
                     TSQRYENRNLEIANISRSLKLLAKEMECPVYALSQLNRSLEQRANKRPINSDLRESGS
                     LEQDADVILFIYRDEIYDADSPFKGTAEIIIGKQRNGPLTSVMTNFRSEFALFENRTN
                     TTH"
     gene            3315..4964
                     /locus_tag="PWY43_002007"
     CDS             3315..4964
                     /locus_tag="PWY43_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL2136861.1"
                     /translation="MTKDEKRLAAIAKGLNVSPISNTAPSYQTATPTHYSSDVEYITV
                     TLDKLRPYEHNPRKTRNPNFEMIKESIRRRGLDHKPNITRRPGEDFYIVADGGNTRIQ
                     ALKELFTETKDPKFWSISCQYKPWQGDTADSVEAELNLLIGHLIENDTRADLSFIEKA
                     LGILQAKEYYEKKLNKSLSARELATELEGDGYIIHYTLANKMERCVLYLYPHIPEVLF
                     KGLGHTQIDKLLAIRNNAEEVWAAHQFDTDINFESLWSENLSKFNEATPFQAKEFQSE
                     LITAMVEALDGKVSFETLYLDIDLDEQKFKKIAAKQQEIDNLVEKTVDQINTRQEQQA
                     AKFEPVIKNENKSVATEPAKNVIDDVIDDNESDDEQNNAITDLSQLDSPLLDKQDNQT
                     EDYLSQIAHNFGLTPGMSIQEQREQRAEANGLTFACEGRQPVEDIWLIHPARQYRSEA
                     YSLALDIAESVGIDHLVEHVVKQPVDYTYAMKPTDSELSPSVLTLYSFLSMLQTAKHK
                     DENNCVLDSNFLFELDDITLVKLFRLIRVSRHIHQQGGKND"
     gene            4957..5514
                     /locus_tag="PWY43_002008"
     CDS             4957..5514
                     /locus_tag="PWY43_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDL2136862.1"
                     /translation="MIKTITVNEALIGNALLNIKEGNIHPCLKMGFSEEQLKAINNLS
                     LDEIMDISNSVVSFAKVEINHETFWKLLAIAQANTQQRQIIDRALLLGASIEMLHQYF
                     GLSTSEVSARRQLLGIEEKMGRKAAASDEESTHIWEIWQKYKQKMESLDSQEGLELLC
                     LIAEEGNMNLTVVWKLVSEWQSKHK"
     gene            5687..>6704
                     /locus_tag="PWY43_002009"
     CDS             5687..>6704
                     /locus_tag="PWY43_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136863.1"
                     /translation="MFSSINNPTEHGLLFFGNQHETVPTRLLQDPCLTPRAKFAWQLI
                     KCNVQAFQGGLFPSYETLSKMLSDRPYAEAKLSEKVVTQTLYLLRLTRWLTLCETVRD
                     ERGYVQGNVYILHDEPVPILDAIQLNSDYLSFLDKCTKHKDSLINGVANHIVESLMAD
                     KTKWHYVSHLDCLQNRYKTYQQQQQMKDKPLSQAEQELSKIQQNILTSAMEVRDFDEE
                     VRKKSSEILTSNKEVRDENDDKSLILDLLPLGKSDVLYSTLYINKYCTGEPSEITWPQ
                     EIPLTSLEQKLTAQAMKGLDLGLCQAILLEAQQRIIQKQDVRKPKGYLYTLIQRAKQG
                     EFKPY"
BASE COUNT         2207 a         1301 c         1311 g         1885 t
ORIGIN      
        1 gagtttggaa ccataaattt aaccgtttcc aacaactccc cttacgcctg aatgctcagc
       61 atccaagcaa acaaattcaa tcgcaacagc aacgttttga ttatatggaa taccgcctcg
      121 agcaggcaat ttatgcactt gttgcagcca aaaagcaggt ggggaaagag ctaaatgtcc
      181 gtttaattca ccaacatccg caaaagcaga ttgaacgcca atcccaaatg cttaagcaat
      241 tggagctgaa actccattgg caaatggagc aacaactcag caaaaaacag caaaaatgga
      301 aaaacattac gcaacggttt gaacgcaacc cgctacctta tcaactttat cagcaacaaa
      361 cgatggtggc aaaaatgtcg caacgcttgg cttattctat cgaaaaaata atgctgaaag
      421 agcagcgaca ttttcaagag ttatgcacta aacttgatgg acttagccca ttgaaaatct
      481 tgaccagagg ctactcaatt acccaaacta aagacggtaa aacgcttact tcaacagaaa
      541 atattgaacg tggtgaggct attatcaccc aactaaaaac aggaaaaata ataagtcaag
      601 tagtggattt tttgtagggg aaattttgaa cgctgccaat ttttaaaatt atttatagta
      661 aaatcagtag gttaaatatt tataaacagg gttgttcact tagttatcca caggtgatgt
      721 gcataactat ttttgtaaag atgaattgag tgctgatttt taaatcaacc gatttcaaaa
      781 ggcattttta cgcttgcaca agcggtcata tttccctata atcgtgcaaa aattaagcca
      841 gtgtggcgaa atcggtagac gcagcggatt caaaatcttt tgcgtaaact gataaaacat
      901 acaggcaatt gaataaaaat gcactctcag cctgagtgat gaaattggta gacatgatgg
      961 attcaaaatc cattggtgaa taaccgtgtc ggttcgagtc cgacctcagg caccattcaa
     1021 ctgcgttcct ttcaaataat actcactcta aatatacagt ttataatgcc aatactcctt
     1081 aacttcaact ttgttaagag agtaaattat gaaaccatta caaaacggta caaacaaacc
     1141 ttttaccatt actattgctt cgaccaaagg aggctctgct aagagtacga atgccgctaa
     1201 cattggtgca ttctgtgcag aacacggtct taaaaccctt cttatcgata ctgataccca
     1261 accgacatta agttcctatt atgaactcgc atatcaagtt ccgggcggaa cttatgaatt
     1321 tcttcatttt aaagatgtag agcctactca tatcatttct aaaacgacta ttcctaatct
     1381 tgacttgatt cagtcaaatg atccgtctaa caaaatcagc ccaatgctga gagattctcc
     1441 tgatggagca ttacgtttca gtttactact acaaaaaata gaaggctacg atgtcatcat
     1501 tattgatacc cgtggaacaa gagatattac tgtcgatatg tctgtgctgg cagctgatgt
     1561 tttattctgc cctattcttc cgcacattct atctgctaag gaatttatcc gtggcacgat
     1621 tggaatgtat caagatttag aaacttttac tgcatttggc tttactctcc caccgctgaa
     1681 agcgatgatt aactgtgttg atcataccaa ggatgtcaaa ttagtctctg actatctgca
     1741 cacgttgttc caaaatgagt ttgatgaaaa caaaacatta ctggaatttt ctgtgcctga
     1801 tcgtgtggca taccgagaag cggcaacttt ttcaaaaccg gtctatcgcc agagccaagc
     1861 tgaatatgaa accattcgtc agctttgtgc attactgatg ccacagtttg cacaatctca
     1921 tttttctgag tagttggggg atttatgtct aacccaaata aaccaaccac ccatctctat
     1981 tcagcagaag ctgaagccgc tgtcatcggg ggattgttat tagataacac tctctttgac
     2041 gatgtcattg gcaagattaa ttcggctgac ttttattttg gtgtgcatca agccttattc
     2101 aaggggatta cggatttaat tgaggtgggg aaacctgcag atattctcac attagatgaa
     2161 tacctaaaac agaaaaatct gttgaaggaa atcggcggtt ttgcctatct tgcagaagtt
     2221 tcaaaaaaca ccccttcggc agccaatgtt ggagcttatg ctgatgtcgt tctgctacat
     2281 agtaaacacc gacaactttt aaagctgggc cagtttattg tcgatcagac tcagacagca
     2341 aaaacaccgg aaaaacttga atcagttatt gatgaagttg agaaaaaaat gactgaattc
     2401 agcctttctg acaataccaa atctgcaaca gatttaagtg atatttttgc cagtatgctc
     2461 tctcgtatgg agctttcggc taaaaatggt gatcctgtga ctggaacacc gacaggtatt
     2521 caaggcatcg atgaagcgac taccggtggg caaccgggtg atttaattgt gattggtgcc
     2581 cgtccttcga tggggaaaac ggcattttct caaactattg cctaccacac ccttgaaaaa
     2641 tttgagtctg ctcctgtgtt ctatcacagt atggaaatgc cggcagatca aattttacaa
     2701 cgttttttag caatgcgtgc cagagtaagt ttacaagata ttcgccaagc agaccgcttg
     2761 gacgatgaag attggggaaa actaacgatg gcaatggggc atattcttga acattggaaa
     2821 aatcgtttag tcattgatga tgaaggtagc cttacccctc aaaaattacg caccaaagtt
     2881 cgacaaaatg ctcgtaaata tggaaaaccg gccgcaattt tcattgatta cattcaacta
     2941 atgcaaacct cgcaacgtta tgaaaatcgc aatttagaga ttgctaacat ctctcgttcg
     3001 ctcaaattac tggcaaaaga gatggagtgc ccagtgtatg cactctctca gcttaatcgc
     3061 tctttggaac aacgtgctaa caaacgtccg atcaactctg atttacgaga atctggttct
     3121 cttgaacaag atgccgatgt cattttattc atctatcgag atgaaattta tgatgcggat
     3181 tcccccttta aaggtaccgc agaaatcatt atcggtaagc aaagaaatgg accacttact
     3241 tctgtaatga ccaattttag aagtgaattt gctctatttg aaaaccgaac aaacacaacg
     3301 cattaggata gattatgaca aaagatgaaa aaagactggc agcgatagcg aaaggattaa
     3361 acgtttcgcc tatttcgaat actgctccat cctatcaaac agcaacacca acgcattata
     3421 gtagcgatgt tgaatatatt acagtgacgc tagataaatt acgtccatat gagcataacc
     3481 ctcggaaaac ccgtaaccct aacttcgaga tgatcaaaga gtctattcgt cgtcgaggat
     3541 tagatcataa gccgaatatc acccgtcgtc cgggagaaga tttttacatt gtcgctgacg
     3601 gtggtaatac ccgtattcag gcacttaaag agctgtttac tgagaccaaa gatccaaaat
     3661 tttggtccat cagttgtcaa tataaacctt ggcaaggtga tactgccgac agtgtcgaag
     3721 ctgaactcaa tttacttatc gggcatttga ttgaaaatga tacccgtgcg gatttgtcat
     3781 ttattgaaaa agctcttggt attttacagg cgaaagaata ttacgagaag aaattgaata
     3841 aatcactttc agcaagggaa ttagctactg agttagaggg tgatggctat attatccact
     3901 atacgttggc taataaaatg gagcgatgtg tactttatct atatccccat attccagaag
     3961 ttctatttaa aggtttagga catactcaaa tcgataagtt attagctatt cgtaataatg
     4021 ccgaagaggt atgggcagcc catcaatttg atactgatat taattttgaa tcgctctggt
     4081 cagaaaattt atccaaattt aatgaggcta caccatttca agcaaaagaa ttccaaagtg
     4141 aattgattac ggcaatggtt gaagctttag atggaaaagt gagttttgaa acactttatc
     4201 tggatattga cttagatgaa caaaaattca aaaaaatagc ggctaaacaa caagaaattg
     4261 ataatttagt tgaaaaaaca gtcgatcaaa tcaataccag acaggaacaa caagcagcaa
     4321 aatttgaacc agttatcaaa aatgaaaata aatctgttgc cacagaacca gccaaaaatg
     4381 ttattgatga tgtaatagat gataatgaaa gtgacgatga gcagaataac gccattactg
     4441 atttatctca attggattct ccattattgg ataagcaaga taatcaaact gaggattatc
     4501 tctcccaaat tgctcataac tttggactca caccgggaat gagtattcaa gaacagcgag
     4561 agcaacgagc agaagccaat ggacttactt ttgcttgtga aggtcgccag ccggttgaag
     4621 atatttggtt gattcatcct gctcgtcaat atcgttcaga ggcgtattcc cttgccttag
     4681 atattgcaga gagtgtgggc attgaccact tggttgaaca tgttgtgaaa cagcctgtgg
     4741 attacactta tgcaatgaaa ccgacagata gtgaactttc accgtctgtt ctaacactct
     4801 attcattcct ttcaatgtta cagacagcta aacataagga tgaaaataac tgtgtcctag
     4861 attccaactt cctgtttgag cttgatgata ttaccttagt caaacttttc cgtctcattc
     4921 gggtcagtcg ccatattcat caacaaggag gaaaaaatga ttaagacaat taccgtaaat
     4981 gaagcattga ttggtaacgc cctgctcaat attaaagaag gaaatatcca tccttgttta
     5041 aaaatgggat tttctgagga acaactaaaa gcgatcaata atctcagtct tgatgaaatt
     5101 atggatatca gcaactctgt tgtgtctttt gccaaagtgg agattaatca cgaaaccttt
     5161 tggaaattgt tggcgattgc acaagctaat acccaacaac gacagattat tgaccgagca
     5221 ttattgcttg gagcttctat tgaaatgtta catcagtatt ttggtttatc aacctctgaa
     5281 gtttctgcac gtcgccaatt acttggtatt gaagaaaaga tggggcgaaa agcagccgca
     5341 agtgatgaag aatctaccca tatttgggag atttggcaaa aatacaaaca gaaaatggaa
     5401 agcctagatt cacaagaagg cttagagctc ctgtgcctga ttgctgagga aggtaatatg
     5461 aatttaacgg ttgtctggaa attggtttcc gagtggcaat caaaacataa gtaaaaaata
     5521 aatgccccaa agttggcgct ttggggcatt cgatattgga gtcttatatc tctattctat
     5581 ggctcataga cttttaggag accgactttg catgagtcct gagtattata gccataggat
     5641 agagaatagc aagagaaaaa ctctttaatc cgaatggaga gaaacaatgt tcagcagtat
     5701 aaataaccca acagaacacg gattgctatt ttttggcaac caacacgaaa cagtacctac
     5761 tcgcttatta caagatccct gtctaacacc aagggcaaaa tttgcatggc agttgattaa
     5821 gtgtaatgtt caagcctttc aaggtggctt atttccttcc tatgagacac tcagcaaaat
     5881 gttgtctgat agaccctatg ctgaggctaa attatcggaa aaagtagtga ctcaaaccct
     5941 ttatttactc cgtcttaccc gatggctgac attgtgtgaa accgtacgtg atgagcgagg
     6001 ttatgtgcaa ggtaatgtgt atattctgca tgatgagcct gtacccattt tagatgctat
     6061 tcagttgaat agcgactatc tctctttctt agataaatgc accaagcata aagatagtct
     6121 tattaatggt gtagcaaatc atattgtaga aagtctgatg gcagataaaa ctaagtggca
     6181 ttatgtctct catttagatt gtctgcaaaa tcgttataaa acatatcaac agcaacaaca
     6241 aatgaaagat aaacctcttt cacaagctga acaagaattg agcaaaattc aacaaaatat
     6301 cctgacttcc gctatggaag tcagggattt tgatgaggaa gtcaggaaaa aatcctcaga
     6361 aatcctgact tccaataagg aagttaggga tgaaaatgac gataagtcct tgattttaga
     6421 cttacttcca ttagggaagt cagatgtact gtacagtact ttatatatta ataagtactg
     6481 tactggggaa cctagcgaaa ttacttggcc acaagaaatc ccactaacct cacttgaaca
     6541 aaaactcact gcacaagcaa tgaaagggtt agatctgggg ttatgccaag ctattttgct
     6601 ggaagcacaa caacgcatta ttcaaaagca ggatgtgaga aaaccgaaag gttatctcta
     6661 tacgcttatc cagcgggcga agcagggtga atttaaaccc tact
//
LOCUS       DANKBV010000125         6527 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.125, whole genome shotgun sequence.
ACCESSION   DANKBV010000125 DANKBV010000000
VERSION     DANKBV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.125"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(233..1618)
                     /gene="hflX"
                     /locus_tag="PWY43_002010"
     CDS             complement(233..1618)
                     /gene="hflX"
                     /locus_tag="PWY43_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL2136864.1"
                     /translation="MLNESLPNSEENSAFGFALSEASQTTEAASPTDPENQDKAILVH
                     LYLGQHKDVENLLEFKTLAESAGVEVLATLTTSRQSPHIKYYVGQGKAEEIAEAVEQL
                     GATVVLVNHQLSPAQTRNLQSLCGCRVVDRTGLILDIFAQRARSHEGKLQVELAQLRH
                     LSTRLVRRVTNQDQQKGGAVGLRGPGETQLETDRRLIKVRIQQLLNRLEKVSKQRNQN
                     RQTRQKADIPTVSLVGYTNAGKSTLFNAITQAGVYAADQLFATLDPTLRRIQVQDVGT
                     TILADTVGFIRFLPHDLVSAFKSTLQETTEATLLLHVIDGSDERKNENIDAVNQVLDE
                     IGALDIPTLLVFNKIDKLEDVEPHIERNADAMPVAVYLSAQENSGIELLFQAIQERLR
                     SNLVCENLLLPVTAGAIYAQFKTENCIKTEHFNEFGERMIYIEINEVQWNKWVKQFPD
                     LAEYIELAKWG"
     gene            complement(1638..1910)
                     /gene="hfq"
                     /locus_tag="PWY43_002011"
     CDS             complement(1638..1910)
                     /gene="hfq"
                     /locus_tag="PWY43_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDL2136865.1"
                     /translation="MAKGQSLQDPYLNALRRERIPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPARALSHNNNNQAQHAQPAAEVTEQAE"
     gene            complement(1979..2932)
                     /gene="miaA"
                     /locus_tag="PWY43_002012"
     CDS             complement(1979..2932)
                     /gene="miaA"
                     /locus_tag="PWY43_002012"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL2136866.1"
                     /translation="MNKKPLALFLMGPTASGKTDLAIALRQELPIEVISVDSALIYKG
                     MDIGTAKPSKEELALAPHRLIDILDPAESYSAMNFHADALREMADITSQGKIPLLVGG
                     TMLYYKALLEGLSPLPSADPAIRAEIESRAAQHGWATLHQELVKIDPIAGARINPNDS
                     QRINRALEVFYITGKTMTELTAQQGEALPYNILQFAIAPQDRAVLHQRIEQRFHKMIE
                     LGFEEEVKRLFLRKDLHINLPSIRCVGYRQMWEYLQGDISLDEAIFKGICATRQLAKR
                     QITWLRGWQGELTWLDSLAPTSSKAKMVEKIDHYLKNCDNI"
     gene            3378..3644
                     /locus_tag="PWY43_002013"
     CDS             3378..3644
                     /locus_tag="PWY43_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2136867.1"
                     /translation="MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATE
                     KRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADLMEQLNARD"
     gene            3631..3900
                     /locus_tag="PWY43_002014"
     CDS             3631..3900
                     /locus_tag="PWY43_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL2136868.1"
                     /translation="MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYC
                     DHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLRIGSHSHLFG"
     gene            complement(3919..5802)
                     /gene="mutL"
                     /locus_tag="PWY43_002015"
     CDS             complement(3919..5802)
                     /gene="mutL"
                     /locus_tag="PWY43_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL2136869.1"
                     /translation="MINTTPKQLIHILPPQLANQIAAGEVVERPASVVKELVENSLDA
                     GANTIQIDIEKGGSQLIRIRDNGCGISKQDLPLALARHATSKISSLEDLEAILSLGFR
                     GEALASISSVSRLTLTSRTAEQTEAWQAYAQGREMAVEIQPASHPIGTTIEVANLFFN
                     TPARRKFLRTDKTEFAHIDEVVRRIALAKPNISFSLSHNGKIVRQYRKVQDNSVEQQQ
                     KRVAAICGDEFIQHSTYIDWQHGDLHLHGWVGSPALARLQNDLCYSYVNGRMMRDKTI
                     NHAIRQAYGDSIASGYYPAFVIFLDLDPTMVDVNVHPAKHEVRFHQGRLVHDFILQGV
                     LNSLQQTQPTLELRNEVNEPLPSYIQDTNRQAAGQNIFVSQTAPQAAEFTQKNASRSS
                     YTPKFERSNSVSSSAQKWYKELVGGDLHQTTPTIKTQSTLVEPKTEPQTEHKTEAVSQ
                     KITQIAPLASHHSQALAIVKNQAVLIKEEENFYLLPLATLAELKLKLQLEKGQSQALL
                     IPLNLSLDQQQTERWQQTKEELTELGFSISEKQWQAQTRLTVLSVPQAVREQNLQQLL
                     FALFNQTQAVNLAEFFAKKCEKPTACTLSDVIALLSEIELYANGKKELEGIRKEVDFS
                     KFL"
     gene            6086..>6527
                     /locus_tag="PWY43_002016"
     CDS             6086..>6527
                     /locus_tag="PWY43_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL2136870.1"
                     /translation="MQMIELAQQAKQASFELAQFGNLQKNQALLTIADSLEQRAEEIL
                     YANAKDIEFAQAQGISLAIIDRLLLNEDRLKDIANDVRNVAKLADPVGQVMDGGVLDS
                     GLKIERQRVPLGVILTIYEARPNVTIDVASLCLKTGNAVILRGGK"
BASE COUNT         1686 a         1342 c         1480 g         2019 t
ORIGIN      
        1 tatcagtggt agtaactgct tgattaagct tttcttcccc acttttgggg aagaataaat
       61 aaaatccttc aaatagaacg gggaaatctg gtttatttga gggatttttt attggctaaa
      121 ttggatttaa tggataaaac aagcggtcaa ttctttacac ttttttgcaa aaaaacgtaa
      181 agaattgacc gcttgcataa tttaaatata acttactcag aagtgagatt aattaccccc
      241 attttgccag ttcaatatat tcggctagat ccggaaattg tttcacccat ttgttccatt
      301 gcacttcgtt aatttcaata tagatcatcc gttcaccgaa ctcattaaaa tgctcggttt
      361 taatgcagtt ttctgtttta aattgtgcgt aaattgcacc ggcagtaacc gggaggagca
      421 agttttcaca cactaagtta ctgcgaagcc tttcttgaat ggcttggaat aacagctcaa
      481 tgccgctatt ttcttgggcg gaaagataca ccgcaactgg catagcatca gcattgcgtt
      541 caatatgcgg ttctacatcc tctaatttat cgattttatt gaatactaat aaagtaggaa
      601 tatctaacgc tccgatttcg tccagcactt gatttaccgc atcaatattt tcgtttttgc
      661 gttcgtctga gccgtcaatg acgtggagca gtaaagtagc ttcggtggtt tcttgcagag
      721 tagatttaaa ggctgaaact aaatcgtgcg gtagaaaacg gataaaacct accgtatcgg
      781 caagaattgt cgtgccgaca tcttgcacct gaatccggcg taaagtcggg tcaagggtcg
      841 caaaaagttg atcggcagca taaacaccag cttgggtaat cgcattaaac agggtagatt
      901 tgccggcatt ggtatagccc actaaggata ctgttggaat atcggctttt tgtctggttt
      961 gtcgattttg attgcgttgc ttgctgactt tttccaagcg gttgagtaac tgttggattc
     1021 gcactttaat caaacggcga tcggtttcga gctgtgtttc acctggacca cgcaagccaa
     1081 ctgctccgcc tttttgttgg tcttgattag taactcggcg aaccaaacgg gtcgataaat
     1141 ggcgaagttg tgccagctcg acttgtagtt tgccttcgtg cgatcttgcc cgttgagcga
     1201 aaatatccaa aatcaagccg gttctatcca ccacacggca gccgcataac gattgcaaat
     1261 tacgggtctg agccgggcta agttgatggt tcaccagcac tactgttgcc ccgagttgtt
     1321 ctaccgcttc ggcaatttcc tccgctttgc cttgccccac ataatatttg atatgcggac
     1381 tttggcggct ggtggtaagc gttgccagca cttcgacacc ggcagattct gccagcgttt
     1441 taaattccag caaattttca acatctttat gttgcccgag atagagatgc accaaaatgg
     1501 ctttatcttg attttctgga tcggtaggcg aggcggcttc tgtagtttgg cttgcttcag
     1561 ataaagcaaa gccaaacgca gaattttctt ctgagtttgg caatgattcg tttaacattg
     1621 gttgagtaat aatgttatta ttccgcttgt tcagttactt cggcagcagg ctgagcgtgt
     1681 tgggcttggt tgttattgtt atgcgataac gcacgggcag gaacaaccgt tgaaatagcg
     1741 tgtttataca ccatttggct tacagtgttt tttaataaaa tcacaaattg gtcgaacgac
     1801 tcgatctgac cttgtaattt aatgccgtta actaaataaa ttgaaacagg aatacgctca
     1861 cgacgaagtg cattcaaata tggatcttgt aaagattgac cttttgccat tgttattttc
     1921 ctttttatag ttgtgtgatt tttaagacac caaataatgc gataagtgcc aaaggcgttt
     1981 agatattatc acaattcttt aaataatgat cgatcttttc caccattttt gctttagaac
     2041 tggtcggagc taaactatct aaccaagtca gttcgccttg ccagccacgc agccaagtaa
     2101 tctgccgttt tgccagctgg cgggtggcac aaatgccttt aaagatcgcc tcatcgagcg
     2161 aaatatcgcc ttgcaaatat tcccacattt ggcggtagcc cacacagcga atcgagggca
     2221 aattaatatg caaatctttg cgtaaaaata accgcttgac ctcctcttca aagcctagct
     2281 caatcatttt gtgaaagcgt tgttcaatcc gttggtgtag aactgcacga tcttgaggag
     2341 cgattgcaaa ttgtaagata ttataaggta gagcctcacc ttgctgagca gtaagttcgg
     2401 tcatcgtttt gccggtaatg taaaaaactt ctaacgctcg gttgatccgt tggctgtcat
     2461 ttgggttaat tcttgcaccg gcaatcgggt cgattttcac taattcttga tgcaatgttg
     2521 cccagccgtg ttgtgccgct cggctttcga tttcagcccg aatggctgga tcggcagagg
     2581 gcaggggaga taacccttca agcaatgcct tgtaataaag cattgtgcca cccaccaaga
     2641 gcggaatttt accctgtgag gtaatatctg ccatttcacg caacgcatca gcgtggaaat
     2701 tcatcgccga atagctctcg gcagggtcta aaatatcaat caagcgatgc ggtgcaaggg
     2761 caagctcttc cttgcttggc tttgccgtgc caatatccat tcctttgtaa attaaggcag
     2821 aatcgacact gattacttca atcggcaatt cttggcgtaa agcaatagct aaatcggttt
     2881 tcccggaggc agtcggcccc attaaaaaaa gggcaagggg ttttttattc atattaatat
     2941 actgtatata gatacaaaaa aagccgtgaa attcacagct tttttgttac aatcctgatt
     3001 gtgctactgc tactggtagg ctcgttaggg ctattcgcag tagcctgagc ctaaatcatc
     3061 cttagtagca ctaaggaggc ttacaatgaa agaactacta ttgattttag ttgcacttgg
     3121 gtttctgtgt gcagcagttg aaattttcac gctctttcaa ctaatctatc tgttcttgta
     3181 agcggctaga tgctaggggc gggttcgtgt tccgcccaga cttctttata ttaccttgat
     3241 ttcttgagta actcaatgat ttcttgtaac gagctaattt taactattca tttcttttct
     3301 ttgaatttct tttttattgc acaatttttt gttaaatgtt attttgtata tcatttgata
     3361 tacaaaagga gtttttgatg gcaaatagtg cattagtaag cgtaagaatc agccctgaaa
     3421 ttaaagagca agcaagtgag gtgttagccg gtatcggctt gactgtctct gatgtgatgc
     3481 gaatgacatt agcgaaaatc gcaacagaaa aacgttttca gtttgattat cagcctaatt
     3541 ttgagacagc agaagtatta agggctgtta agaatggcga ggaaaagcta cattcagcaa
     3601 atagtattgc ggatttaatg gagcaactca atgcgagaga ttgagtatag taatgctttt
     3661 aaacgtgact ataaaaaatg tgcggttgag tctttatcct cttcattaat tgaggcgttg
     3721 tattttctga ttaatgacaa gccattacct gagaaatact gcgatcacgc tttaaccggc
     3781 gaatggaaag gttttaggga ttgccatatc caacctgatt tggttcttat ctatcgaaaa
     3841 gtggaagatg ataagctaga gctgctgcga attggcagcc actctcattt atttggctaa
     3901 agggtattgc cctttagact ataaaaactt ggaaaaatcc acttccttcc ttattccctc
     3961 cagctctttt ttcccattgg cataaagctc aatttctgag agtagagcaa tcacgtcact
     4021 tagcgtacaa gcggtgggtt tttcgcattt ttttgcaaaa aattcggcta aattgaccgc
     4081 ttgtgtttgg ttgaacagag caaataaaag ctgttgcagg ttttgttctc tgacagcctg
     4141 tggtacggaa agcactgtga gccgtgtttg ggcttgccac tgtttttcgc tgatcgaaaa
     4201 gccaagctca gttagttctt ctttggtttg ttgccagcgt tcggtttgtt gttgatctaa
     4261 actgagattc agtgggatca acaacgcttg agattgccct ttttccagtt gtagcttgag
     4321 tttaagctcg gctaatgttg ccagtggaag caggtaaaaa ttttcctctt ctttaattaa
     4381 caccgcttgg ttttttacaa tcgctaaggc ttgtgaatgg tggcttgcaa gcggtgcaat
     4441 ttgcgtaatt ttttgcgaaa cggcttctgt tttatgttca gtttggggtt ctgtttttgg
     4501 ctcaacaagc gtgctttgcg ttttgatagt tggcgttgtt tggtgcaaat cgccaccaac
     4561 caattcctta taccattttt gagcggacga actcacactg ttgcttcgtt caaatttagg
     4621 cgtatagctt gatcgacttg catttttttg cgtaaattcg gccgcttgtg gagcggtttg
     4681 cgacacaaaa atattttgcc ccgcagcttg gcggttagtg tcttgaatat agctcggtaa
     4741 cggctcgttc acttcatttc gcagttcaag cgtaggttgg gtttgttgca ggctgttgag
     4801 tacgccttgc aaaataaagt cgtgtaccag tcttccttga tgaaagcgca cctcgtgttt
     4861 agccgggtga acgtttacat caaccattgt cggatctaaa tccaaaaaga tcacaaaagc
     4921 cggatagtaa ccacttgcaa tgctgtcgcc gtaggcttgg cgaatggcgt gattgatggt
     4981 tttatcccgc atcattcgcc cgttcacata gctgtagcaa aggtcgtttt gcagacgggc
     5041 aagagccggt gagccaaccc agccgtgcag gtgcagatcc ccgtgttgcc agtcaatata
     5101 agttgaatgt tgaataaact catcaccgca aatggctgca acccgttttt gttgctgctc
     5161 taccgaattg tcttgcactt tgcgatattg gcggacgatt ttaccattat ggcttaagct
     5221 gaaactgata ttcggcttgg caagggcaat gcggcgaacc acctcgtcaa tatgggcaaa
     5281 ttcggtttta tcggtgcgta gaaatttacg ccgtgccggt gtgttaaaaa acagatttgc
     5341 tacttcaatg gttgtgccaa tcgggtgtga ggcaggctga atttccaccg ccatttcccg
     5401 cccttgtgca taggcttgcc atgcttcggt ttgctctgcg gtacgagagg tcagtgttaa
     5461 acgtgaaacc gagctgatac ttgccaatgc ttcgccccga aagcctaaac ttaaaatggc
     5521 ttctaaatct tccaagctgc tgattttgct cgtagcgtgg cgagcaagtg ccagcggtaa
     5581 atcttgcttg ctgataccac aaccgttatc tcgaatgcgg ataagctgag agccgccttt
     5641 ttcaatgtcg atttgaatag tattggctcc ggcatctaaa ctgttttcca ctagctcctt
     5701 taccacagac gcagggcgtt ccaccacctc gccggcagca atttggtttg ccagttgagg
     5761 cggaagaatg tggattagct gtttaggggt agtgtttatc attttgttta agttttatat
     5821 gtttttaccc ctctctctgc ctacagattc tgaacgaatc tgtaggcggt ctctctccca
     5881 caaggggaga gagttaaatt ctaccctctc cccttgtagg agagggatag atttttctcc
     5941 gttcaggaga aaaatcaggg agaggggaat tgcgtaatgt tttaaaattc ccccatttta
     6001 tccaattttc ttggcttttg catatttctt cacataaaag aacgtgttag attatggaga
     6061 atattgatta aatgagacct aatttatgca gatgatagaa cttgcccagc aagccaaaca
     6121 agctagtttt gagctggctc aatttggcaa cctacagaaa aaccaagcct tactcacgat
     6181 tgccgatagc cttgagcaac gtgcagaaga aattttatac gctaatgcca aagatattga
     6241 atttgctcaa gcacaaggga tttcattggc gattattgac cgcttattat tgaatgaaga
     6301 tcggctaaaa gacattgcta acgatgtgcg aaatgttgcc aaattagcgg atccggtggg
     6361 gcaggtgatg gacggtggcg tgttagacag cggtttgaaa atcgaacgcc agcgtgtgcc
     6421 gctcggggtg attttaacca tttacgaagc tcgcccgaat gtgaccattg atgtggcatc
     6481 attatgccta aaaaccggca atgcagtgat tttgcgtggc ggtaaag
//
LOCUS       DANKBV010000126         6407 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.126, whole genome shotgun sequence.
ACCESSION   DANKBV010000126 DANKBV010000000
VERSION     DANKBV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.126"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            37..987
                     /gene="lpxM"
                     /locus_tag="PWY43_002017"
                     /gene_synonym="msbB"
     CDS             37..987
                     /gene="lpxM"
                     /locus_tag="PWY43_002017"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249087.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDL2136871.1"
                     /translation="MTRITAQDGYTHKFLWSFLHPKYWGVWLGVLGLVILAYVPVRIR
                     DKLSAFVGKMAYRYLKQKNKKGYHRAKVNLRYCFPDWSEKKREEVVEKMFITVAQTML
                     AIGETAIRPASYLQKRCEFTGFENVVKAKESGKNVIMLVPHTWSIDMAGVAMFSLGYP
                     MTSMYNPHRNALVDWLWNITRERLGGRMHTRQNGIKAFLADVRKGDVGYFLPDEDFGE
                     ELSVYADFFATEKATLPGLNKMARVANAEVIPMFSIYNAEKAKYEMEILPPLEFSGDP
                     IQSAREMNRVIEYFVEKNPEQYVWILRLLKTRRNREDIYL"
     gene            1115..1654
                     /gene="apt"
                     /locus_tag="PWY43_002018"
     CDS             1115..1654
                     /gene="apt"
                     /locus_tag="PWY43_002018"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL2136872.1"
                     /translation="MNQLDLIKSSIKSIPDYPKAGIIFRDITSLLEVPTAFQATIDAI
                     VAEFKDKGITKIVGTESRGFIFGAPVALALGVPFVLVRKPKKLPREVISQSYTLEYGE
                     DTLEIHKDAVSANDNVLVIDDLLATGGTIDATAKLIRRLGGNVEHAAFVIWLPELGGK
                     ERLEKEGIKSFSLVSFEGH"
     gene            1745..3802
                     /gene="dnaX"
                     /locus_tag="PWY43_002019"
     CDS             1745..3802
                     /gene="dnaX"
                     /locus_tag="PWY43_002019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL2136873.1"
                     /translation="MSYQVLARKWRPQRFSEVVGQQHVLSALENSLREDRLHHAYLFS
                     GTRGVGKTSIARLFAKGLNCVNGITAEPCGECANCKAIEEGHFIDLIEIDAASRTKVE
                     DTRELLDNVQYKPTVGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFLLATTDPQ
                     KLPITILSRCMQFHLRALEQSQIARHLEFILNAENIPFEPLALEKLAKAAQGSIRDSL
                     SLTDQAIAMSNANITLAIVSQMLGLIDDNQPLELVQATAQADGEKAMAIIEQIAAKGV
                     DWQQLLSDVAEVLHKIAMLQLVKTPTTEETLVHFLAKQIPPEDVQFFYQLILNSKKEL
                     PFAPEQRAGVEMAILRALAFHPKQAEKIATVAVEKVTQPNHSVSAVTTAKPQNQISQL
                     RENFEQNQQKTVTVANTTTAVESIAQPPQAVDTAISPSAPATLSPAMAALEARKKLQA
                     QQANLAQTEKKKPELKAPQVIAKKSANQTASLQERFISLAENQHKAVLQVSKKTESAP
                     KSVEDEDYRWTWLNPELENQADSAKPSDIKQAILQERTPELVAKTIHLSCQQDKWCDI
                     ANSLNLGGFSRQIALNSYLAEQNEQHLALVLKPNMAHLDNPESRKNLEAALAKKGYSY
                     ELTIGDNSEQQTPLEIRRAIFKQLTEAAKSALLNDEKLMLLRNAFDAQIDESTIRAVA
                     ETE"
     gene            3808..4716
                     /locus_tag="PWY43_002020"
     CDS             3808..4716
                     /locus_tag="PWY43_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2136874.1"
                     /translation="MKALELIDLVKTYPTGVKAVKGINLVVEQGDFYALLGHNGAGKS
                     TTIGIISSLVNKTSGSVKVFGYDLDTQKVELKHQIGLVPQEFNFNQFEKVIDVLVQQA
                     GFYGIPRSEALVRAEKWLKLLDLWEKRTHLTRELSGGMKRRVMIARALMHNPKLLILD
                     EPTAGVDIELRRSLWDFLRDLNNQGTTIILTTHYLEEAETLCRHIGIIQHGELIENTS
                     MKALLAKLESEVFVLDLQNSEQNQPLVIQNYPHKWLDENTLEVNVKRQQGLTNLFSQI
                     SAQGAEVLSMRNKSNRLEELFINMAQ"
     gene            4718..5479
                     /locus_tag="PWY43_002021"
     CDS             4718..5479
                     /locus_tag="PWY43_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2136875.1"
                     /translation="MNLIGFYTLAYKECRRILRIWRQTLVPPVITTTLYFLIFGTLIG
                     GRIGEMNGVGYTQFIAPGLIMMTAITASYVNTASSFFLSKFTRDFEEMLISPLSTHNI
                     IWGYVTGSILRAGLAATLVMCVAMVFVSFEIYSVAIIVLTLLLTIIAFALGGVINAVF
                     ARSFDDVGLIPTFVLTPLTYLGGVFYSISLLPEFWQTVSKFNPIVYMINGLRYGFLGI
                     SDVPLGYTFAVLTSLCIGLYIIAYSLIQRGVGLRS"
     gene            5500..6255
                     /gene="modA"
                     /locus_tag="PWY43_002022"
     CDS             5500..6255
                     /gene="modA"
                     /locus_tag="PWY43_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2136876.1"
                     /translation="MKALKKLAITMALTLSGTAFSQEITVFAAASMTNVLQDIQQDFE
                     KAYPQDKITFSFASSSVLAKQIEQDAPADIFISADQKWMDYVAEKQPEKVKYRRNLVR
                     NELVLIAPTESNIEPKAIQAVDFAKELANSYLSVGDPNHVPAGKYAKKALSHYQLWGS
                     VEPRLARAKNVRDALSFVERGESPLGIVYSTDAKVSNKVKVVSVFPPESYGEIVYPAA
                     AVSEKASAATFLDFLKTPKAKAKFEAAGFYPVN"
BASE COUNT         1935 a         1327 c         1405 g         1740 t
ORIGIN      
        1 aagcaaagaa agtgacaaat aatgaggaat tatagaatga cacgaattac cgcccaagac
       61 ggctatacgc acaaattcct atggtctttt ctacacccga aatattgggg cgtatggtta
      121 ggtgtattag gattagtgat tttagcctat gtgccggtgc gaattaggga taaattatcc
      181 gcttttgtgg gtaagatggc gtatcgctat ctcaagcaga aaaataaaaa aggctaccac
      241 cgtgctaaag tgaatttgcg ttactgtttt cccgattgga gtgagaaaaa gcgtgaagaa
      301 gtggtcgaga aaatgtttat cacggtagca caaaccatgt tggcgattgg cgaaactgca
      361 atccgcccgg cctcttattt acaaaaacgg tgtgaattta ccggatttga aaatgtggtg
      421 aaagccaaag agagcggtaa aaacgtgatt atgttagtgc cacatacttg gtcgattgat
      481 atggcggggg tggcgatgtt ctcgctcggt tatccgatga catcaatgta caatccacac
      541 cgtaatgcgt tggtggactg gctgtggaat attacccgtg agcgtttagg tgggcgaatg
      601 cacactcgtc aaaatgggat taaagcgttt ttagccgatg tgcgtaaagg tgatgtcggt
      661 tattttctgc ccgatgaaga tttcggcgaa gagctaagtg tgtatgccga cttttttgcc
      721 accgaaaaag ccactttgcc gggcttaaat aaaatggcaa gagtggcaaa tgcggaagtg
      781 atcccaatgt tctcgattta taatgcggaa aaagcaaaat atgaaatgga aattttgcca
      841 ccgcttgaat tttcaggaga cccaattcaa tccgcccgag aaatgaaccg agtaattgaa
      901 tattttgtgg agaaaaaccc cgagcagtat gtttggattt tacgtttgct taaaaccaga
      961 cgaaacaggg aagatattta tctataatat agggaaaagg aaactgaaaa gtaagctgta
     1021 ctatcaaacg taaggttatc aatttctcct tgaaatttct gctgtttttc tatagagttt
     1081 tccagttatt tttttattac acgtaggtca aacaatgaac caattagatt taattaaatc
     1141 ttctatcaaa tccattccag attatccgaa agcagggatt atcttccgtg atatcacttc
     1201 acttttagaa gtgccaactg cattccaagc caccattgat gccatcgttg cagaatttaa
     1261 agacaaaggt attactaaaa ttgtcggcac agaatctcgc gggtttattt ttggtgcccc
     1321 tgttgcatta gcgttaggcg tgccttttgt gttggttcgt aaaccgaaaa aattaccacg
     1381 tgaagtgatt tcgcaatctt acacgcttga atatggtgaa gacacgctag aaattcataa
     1441 agacgcagtg agtgctaatg ataatgtatt ggtcatcgac gatttactcg caaccggtgg
     1501 tactattgat gcaactgcaa aactaattcg tcgcttaggt ggtaacgtag aacacgctgc
     1561 ctttgtgatt tggctgcctg agttaggtgg taaagagcgt ttggaaaaag aagggattaa
     1621 atcctttagc ctagtcagct ttgaaggtca ttaatgggga acaagcggtg gctttttgct
     1681 aaaattttgc aaaatttttc ggaaagatga ccgcttgtga tttccaaatt gaatagaaaa
     1741 atcaatgagt tatcaagttt tagcccgtaa atggagaccg caacgcttct cggaagtagt
     1801 tggtcagcaa cacgttttat ctgctcttga aaacagctta agagaagata gactgcacca
     1861 cgcttacctg ttttcaggca ctcgtggtgt gggtaaaacc tctattgccc gtttattcgc
     1921 caaaggctta aactgtgtaa acgggatcac cgccgagcct tgcggtgaat gtgcgaattg
     1981 taaagcgatt gaagaagggc attttatcga tctcattgaa attgatgctg cttctcgtac
     2041 taaagtggaa gacacccgtg agctacttga taacgttcaa tacaaaccca ccgttgggcg
     2101 ttttaaggtt tatttaattg acgaagtgca tatgctctct cgtcatagtt ttaacgcctt
     2161 actaaaaacc cttgaagagc cgcctgaata tgtcaaattc ttgctggcta ccactgatcc
     2221 gcaaaaactg ccgattacga tcctctctcg ctgtatgcaa tttcatctgc gggcattaga
     2281 gcaatcgcaa attgcccgtc atcttgaatt tattctaaat gcagagaaca ttccgtttga
     2341 gcctttggca ctagaaaagt tggcaaaagc agcacaaggt agtatccgag actctctcag
     2401 cctcaccgat caagccattg caatgagtaa tgccaatatt acgcttgcga tagtcagcca
     2461 aatgctaggg ttgattgacg ataatcaacc actagaatta gtgcaagcaa ccgcccaagc
     2521 cgacggtgaa aaagcaatgg cgatcatcga gcaaattgca gcgaaaggcg ttgattggca
     2581 acaattatta agcgatgtgg cagaagtgtt acacaaaatt gcaatgttgc aattagtgaa
     2641 aacacctaca accgaagaaa cattagtgca ttttctggca aaacaaattc cgcccgaaga
     2701 cgtgcaattt ttctatcaat taatcctcaa cagtaaaaaa gagttgcctt ttgcaccgga
     2761 gcaacgtgcc ggtgttgaaa tggcgatttt gcgtgcgttg gctttccacc ctaaacaggc
     2821 agaaaaaata gcaacagttg cagtggaaaa ggttacacaa ccaaaccatt cggtttcagc
     2881 ggttacgaca gcaaaaccac aaaatcaaat tagccagcta agagaaaatt ttgagcaaaa
     2941 tcagcaaaaa acggttactg ttgccaatac taccacagcg gttgaaagca ttgctcaacc
     3001 gccacaggca gtagataccg ctatttcgcc gtctgctcct gcgactcttt caccggcaat
     3061 ggcagccctc gaagcccgca aaaagctaca agcacagcag gcaaatttag ctcaaaccga
     3121 aaaaaaaaag cctgagctaa aagcccctca agttattgca aaaaaatcgg caaatcagac
     3181 cgcttcattg caagagcggt ttatcagctt ggcggaaaat cagcacaaag ccgtactgca
     3241 agtttcaaag aaaaccgaat ccgcccctaa aagtgtggaa gatgaagatt atcgctggac
     3301 gtggctcaat cctgaattag aaaatcaagc agacagtgcc aaaccttctg atattaaaca
     3361 agcgattttg caagaacgca cccctgaatt agtggcaaaa accattcatt tatcttgtca
     3421 gcaggataaa tggtgtgaca tcgccaactc actcaattta ggcggatttt cccgtcagat
     3481 cgccttaaat agctatttag cggaacaaaa tgaacagcac ctcgcattgg tgttaaagcc
     3541 gaatatggct cacttagata atccggaatc acgtaaaaat ttagaagctg cccttgccaa
     3601 aaaaggctat tcatacgaat tgaccattgg tgacaatagc gaacagcaaa ctccgcttga
     3661 aattcggcga gccattttca aacaattaac tgaggcagca aaatcagctc tgcttaacga
     3721 tgaaaaatta atgttacttc gcaacgcttt tgatgcacaa attgatgaat cgaccattcg
     3781 agcggtggcg gaaactgagt aaaacaaatg aaagcattag aattaattga tttggtaaaa
     3841 acctacccaa caggtgtaaa agccgttaaa ggcattaatc ttgtggtcga gcaaggtgat
     3901 ttctatgccc tacttgggca taacggggca ggtaaatcta ctactattgg cattatcagc
     3961 tcactggtaa ataaaaccag cggcagcgtg aaagtgtttg gctatgattt agacacccaa
     4021 aaagtcgaat taaagcatca aatcggctta gtgccacaag agtttaattt caatcagttt
     4081 gaaaaagtga ttgatgtgct agtgcaacaa gccgggtttt acggcattcc tcgcagtgaa
     4141 gccttagtac gtgccgaaaa gtggctgaaa ttgctcgacc tatgggaaaa acgcacccac
     4201 ctcacccgtg agctttctgg tggaatgaaa cgtcgggtga tgattgcccg agccttaatg
     4261 cacaatccaa aactactgat tttagatgaa cctaccgcag gggtggatat tgaactccgc
     4321 cgctcattgt gggatttctt gcgggatcta aacaatcaag gcacaactat tattctcact
     4381 acccactatt tagaagaggc ggaaacccta tgccgtcaca tcggcattat tcaacacggc
     4441 gaattaattg aaaacacctc aatgaaagcc ttgcttgcca aattggaaag tgaggtattt
     4501 gtgttagatt tgcaaaattc tgagcaaaat caaccgcttg tgattcaaaa ttacccgcat
     4561 aaatggcttg atgaaaacac ccttgaagtg aatgtaaaac gccagcaagg gctaaccaat
     4621 ctgttttcgc aaatttccgc ccaaggggct gaggtgctaa gtatgcgaaa caaatcaaac
     4681 cgcttggaag aactatttat caatatggct cagtaaaatg aatttaatcg gattttatac
     4741 cttagcttac aaagagtgcc gccgcatttt gcggatttgg cgacaaactt tagtgccacc
     4801 ggtgattact accacgctct attttttaat cttcggcacg ctgattggtg ggcgaatcgg
     4861 ggaaatgaat ggcgtgggtt acacccaatt tattgcaccc ggcttaatta tgatgaccgc
     4921 catcaccgcc tcttatgtga ataccgcctc ttcatttttc ttgagtaaat ttacccgaga
     4981 ttttgaagag atgctgattt cgccactctc tacccataat attatttggg gttatgtcac
     5041 cggcagtatt ttgcgtgcag gtttagccgc aaccttagta atgtgcgtgg caatggtgtt
     5101 tgtctcgttt gaaatctact cggttgcgat tatcgtctta accctgttgc tgacaatcat
     5161 tgcctttgcg ttaggtggcg taattaatgc ggtgttcgcc cgctcctttg atgatgtggg
     5221 gctgattcca acctttgtgc ttactccctt aacttactta ggcggcgtat tttactctat
     5281 ttcattactg cctgaatttt ggcaaacggt gtctaaattc aacccgatcg tttatatgat
     5341 taacggctta agatatggct ttctcggtat cagcgatgtg ccgttaggtt atacttttgc
     5401 agtgcttact tcactttgca tcggcttgta tattattgcc tattccttaa ttcaacgagg
     5461 ggttgggctg cgttcttaac taattgtgag gttatgaaaa tgaaagcatt aaaaaaacta
     5521 gcaattacaa tggcattaac gttatcaggc acagcctttt cacaagagat taccgtgttt
     5581 gctgccgctt caatgacaaa tgtgttacaa gatattcagc aggattttga aaaagcctat
     5641 ccacaggata aaatcacgtt ctccttcgct tcatcatcgg tattagcaaa gcagattgag
     5701 caagatgcac cggcagatat ttttatttcc gccgaccaaa agtggatgga ttatgttgcc
     5761 gaaaaacaac ctgagaaggt taaatatcgc cgcaatttag tcagaaacga gttagtatta
     5821 attgcaccaa ccgagagcaa catcgagcca aaagcgatac aagcggtcga ttttgccaaa
     5881 gaattagcaa acagctattt atcggtggga gacccaaacc acgttcctgc cggcaaatat
     5941 gccaaaaaag ctcttagcca ttaccaatta tggggaagtg ttgaacctcg tttagcgagg
     6001 gcaaaaaatg tacgagatgc cctttctttt gtcgagcgag gcgaatcccc gcttgggatt
     6061 gtctattcca ccgacgccaa agtgtcgaat aaagtcaaag tggtttccgt tttcccaccg
     6121 gaaagctacg gcgaaattgt ttaccctgct gcggcagtaa gtgaaaaagc ctctgccgcc
     6181 acatttttag atttcctcaa aactccgaaa gcaaaagcga aatttgaagc agcagggttt
     6241 tatcctgtaa attaaattta ctctctcccc ttgtgggaca aacgcttgcg tttgaacgtt
     6301 ggcttttcca acggctctgt aggagcgagc gaagcgagta tcgagacaga aaaatatgtc
     6361 gttcagacat atttttcaga gagagggcaa atagcgttta agatatt
//
LOCUS       DANKBV010000127         6340 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.127, whole genome shotgun sequence.
ACCESSION   DANKBV010000127 DANKBV010000000
VERSION     DANKBV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.127"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            90..1124
                     /gene="mltG"
                     /locus_tag="PWY43_002023"
     CDS             90..1124
                     /gene="mltG"
                     /locus_tag="PWY43_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL2136877.1"
                     /translation="MLKKILIALGLAGVIACGGLFYGYQKLTSLAEHPITVQPNQLFV
                     LEKGVSSQKLAALLEEQGIVTHDDADLIPYLMRLYPELSKFKAGAYSLAGLTTVKDLL
                     AHLSSGKEVQLNVQFIEGKTFKIWREQLAKADYLQHTLQGKSEKEIADLLGIPHEKIE
                     GWIAPDTYNYTPNSTDLALLKRAYQKQKTALEQAWKNRAENLPLATPYEMLILASIVE
                     KETGIATERPQVASVFINRLRNNMRLQTDPTVIYGMGDRYNGNIRRKDLEEATPYNTY
                     QIDGLPPTPIAMPSEASIKAVANPDNTPYLYFVADGTGGHKFSKTLNEHNKAVAEWIQ
                     IERKRKNEQK"
     gene            1135..1755
                     /locus_tag="PWY43_002024"
     CDS             1135..1755
                     /locus_tag="PWY43_002024"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL2136878.1"
                     /translation="MRGKFIVIEGLEGAGKTTAHQVILKELEQAGINDVVMTREPGGT
                     PLAEKLRHLIKHETEEAINDKAELLMLYAARVQLVENVIKPALAEGKWVLGDRHDMSS
                     QAYQGGGRKLDRHLLETLKESVLGTFEPDLTIYLDLDPVIGLERARGRGELDRIEQQS
                     LDFFYRTRERYLELTQHNDKAVIINAEQPIEQVTADIQQVIRNWLK"
     gene            1755..2732
                     /locus_tag="PWY43_002025"
     CDS             1755..2732
                     /locus_tag="PWY43_002025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL2136879.1"
                     /translation="MYPWLESTYQQLTDSFLQGRGHHALLFKTDVGLGTDTLIYKVAE
                     WLLCSQKLRHQPCLECKSCLLMQSNNHPDFHILEPIEDKDIGIDQVRELIYKLQNFAQ
                     QGGNTVVYIRGVDRLTEASSNALLKTLEEPRENVYFLLEAPLQATVLATIQSRSQTWV
                     LYAPQASEVMAWLQTQFPAISTDDLEIALRLCHSRPLICKNFIENDRLQQRKTFLQTF
                     WRFYKSHDVFLLFQAFAQEKELILQQLEWLESFFSDALKAKMAISHGWLNPDLQNGIL
                     PFSQQLTTHGLLKGHQILQQTQRDLTEINAVNPELMLLDCLTKLVLVFE"
     gene            2863..3105
                     /locus_tag="PWY43_002026"
     CDS             2863..3105
                     /locus_tag="PWY43_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL2136880.1"
                     /translation="MARTTSVTLGEPLNDFVNEMVASGRYGSTSEVVRTALRMLEEKE
                     QYLAQLRQAIDDGDASGYATETIDEMMLRVKAELNV"
     gene            3098..3382
                     /locus_tag="PWY43_002027"
     CDS             3098..3382
                     /locus_tag="PWY43_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2136881.1"
                     /translation="MYKLSAQALRDQEKLFRYSISQFGVQQTESYFADLKHILNLLAD
                     FPTMGTSAEHIRGGLRMHTHQSHTIYYRIKADHIFIVRILHNQMNHKRYI"
     gene            3387..4175
                     /locus_tag="PWY43_002028"
     CDS             3387..4175
                     /locus_tag="PWY43_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HDL2136882.1"
                     /translation="MKDLFIIDSHCHLDSLDYETRHKNVDEVIENAKARGVHHFISVC
                     TTVGRFEAMKNLTAHRNDVSLSCGVHPLNVEDEPFDYDKLFQFAQDPKVVAIGETGLD
                     YHYTPETKALQQSLFVQQIEIANKLNKPLIIHSRSARQDTMDMLEQNNAEKCGGVLHC
                     FTEDWAMAKRALDIGFYISISGIITFRNAEELREVVRKVPLDRLLVETDSPYLAPIPY
                     RGKPNQPAYVRETCEYVATLKGVSTEELARITTENVQHLFNIKL"
     gene            4185..4706
                     /locus_tag="PWY43_002029"
     CDS             4185..4706
                     /locus_tag="PWY43_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HDL2136883.1"
                     /translation="MRAILKYFLILVSLSFFIVLGGAVNYAMPSYEDTVVTGMEVRRM
                     DKDGIISKANPADGEVRDVYFLFTEDPETKKVMVYRNEDTGWGLPPYFKFGSADIQAK
                     AQAYANEKQRVQIKYYGWRINWLNEFRNIVSIKPLEEAETVSKPIMSYVLYAVLAFLF
                     FLSVQFIRGIFKD"
     gene            4781..5482
                     /gene="lpxH"
                     /locus_tag="PWY43_002030"
     CDS             4781..5482
                     /gene="lpxH"
                     /locus_tag="PWY43_002030"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDL2136884.1"
                     /translation="MTYYFIADLHLNENQPEITHLFLQFMQEKALLAEAVYILGDLFD
                     FWIGDDEQSELISTVKQAIKTLTTSGTKCYFICGNRDFLIGKRFSEQTGMVILPDYHV
                     IDLFGTSTLLCHGDTLCTDDVKYQQFRKKVHQKWRQAIFLMFPLSWRIRIAEKIRSKS
                     KQEKRDKSAEIMDVNAEVTAQIVAKYNTTQLIHGHTHRQAIHQHNGFTRIVLGDWKEN
                     YASILSVTEQGAEFI"
     gene            5479..6264
                     /locus_tag="PWY43_002031"
     CDS             5479..6264
                     /locus_tag="PWY43_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2136885.1"
                     /translation="MINVIIPCYNAENTLVRAVQSVLNQSGLNLLWLIDDASTDNTLA
                     LAKHLQAQVPDKIRVEQMPQNGGVAKARNWGALQSETDLIAFLDADDAYENGALQVAE
                     KIFEFKPETGLVRLALKPVNLDQRYSSHPNFELAWQYMQMTGAGNTVFRRSFFLACGG
                     FPQHQLFQELGGEDGALGIATTQISSVATAFNDVGVLHYCREGMHAERLLNAILFNEK
                     DPNVTEEKVKQANLITENIVNNIRNLQDCLNSKGIGIKPLIYA"
BASE COUNT         1993 a         1340 c         1279 g         1728 t
ORIGIN      
        1 actcaaatcc catttgcaaa tttttctgaa aaaacaaccg ctttatagta gaattcagtc
       61 aatttttatc acttttattt tattcatcaa tgctaaaaaa aatccttatt gcattaggct
      121 tagccggcgt tattgcttgc ggtggactgt tttacggcta ccaaaaactc acctctttag
      181 ccgaacaccc gatcacggtt cagccgaatc aacttttcgt attggaaaaa ggcgtttcca
      241 gccaaaaatt agcggctctg ttagaagagc aaggcattgt cacccacgat gacgccgatc
      301 ttatccctta tttaatgcga ttataccctg agttaagcaa attcaaggct ggtgcttatt
      361 cactcgcagg cttaacgaca gtaaaagatt tgctcgctca tttaagctcc ggcaaagaag
      421 tgcaacttaa cgtgcaattt attgagggca aaacttttaa aatttggcgg gaacaactag
      481 cgaaagccga ttatttacag cacacgctac aaggcaaatc ggaaaaagaa atcgctgact
      541 tactcggcat tccacacgaa aaaatcgaag gttggatagc cccggatact tacaactaca
      601 cgcccaattc caccgattta gccttgctta aacgggctta tcaaaagcag aaaacggcgt
      661 tggaacaggc ttggaaaaat agagcggaaa atctaccgct tgcaacacca tacgaaatgc
      721 tgattcttgc ctcgattgtg gaaaaagaga ccggcattgc gaccgaacgc ccacaagtgg
      781 catcggtctt tattaaccgt ttacgcaaca atatgcgact gcaaaccgat ccaaccgtca
      841 tttacggaat gggcgatcgt tataacggca atatccgccg caaagattta gaagaggcaa
      901 cgccatacaa tacttaccag attgacggct taccgccaac gccgattgcc atgccgagtg
      961 aagcttcgat taaagcggtt gccaacccgg ataatacacc ctacctctat tttgtggcag
     1021 acggcacggg cggtcataaa ttcagcaaaa cactgaatga gcataataaa gcggtggcag
     1081 aatggattca aattgaacgc aaacgcaaaa atgaacaaaa atagtgagaa caatatgcgt
     1141 ggaaaattta ttgtaattga aggcttagag ggcgcaggca aaacgactgc tcaccaagtg
     1201 attctcaaag agttggaaca agccggcatt aatgacgtag taatgacccg agaacccggc
     1261 ggcacccccc ttgccgaaaa gttacgccat ttaattaaac acgaaaccga agaagccatt
     1321 aacgataagg ctgagctgct aatgctctac gctgctcgtg ttcaattagt cgaaaatgtg
     1381 atcaaacctg cccttgccga aggcaaatgg gttctgggcg accgccacga tatgtcttcc
     1441 caagcctatc aaggcggtgg acgtaagttg gatcgtcact tactcgaaac cttaaaagaa
     1501 tcggtactcg gcacattcga gccagattta acgatttatc tcgatctcga tccggtgatt
     1561 ggtttagaaa gagccagagg cagaggcgaa ttagaccgca tcgaacagca aagccttgac
     1621 ttcttctacc gcacacgaga gcgttattta gaattaaccc aacacaacga taaagccgtg
     1681 atcatcaatg ccgaacaacc catcgaacag gtgactgcgg atattcaaca agtaattaga
     1741 aactggctga aataatgtac ccttggctcg aaagcactta tcaacaactt accgattcat
     1801 ttttacaggg gcgaggtcat cacgccttac tgttcaaaac agatgttggt cttggtacag
     1861 acaccttaat ctacaaggtg gctgaatggt tgctttgcag ccaaaaactc cgccatcaac
     1921 cttgtcttga atgcaaaagt tgcctgctta tgcaaagcaa caaccacccc gatttccaca
     1981 ttcttgagcc gattgaagac aaagatattg gtatcgacca agtgcgtgag ttgatttata
     2041 aactccagaa tttcgcccaa caagggggaa atacggtcgt ttatattcga ggagttgatc
     2101 gtttaaccga ggcaagcagt aatgccctgc tcaaaacctt ggaagagcca cgtgaaaacg
     2161 tctatttctt gctcgaagca ccgcttcaag caacagtttt agctaccatt caaagccgca
     2221 gccaaacttg ggtactttac gccccacaag cctcagaagt aatggcttgg ctacaaacac
     2281 aatttccggc aatctcaacc gacgacttag agattgcact ccgcctttgc cactcacgcc
     2341 cactaatttg caaaaatttt atcgaaaatg accgcttgca acagcgaaaa acctttttgc
     2401 aaaccttctg gcgtttctac aaaagccacg atgtcttctt gttattccaa gccttcgccc
     2461 aagaaaaaga gctgatttta cagcaactcg aatggctgga aagtttcttt agcgatgccc
     2521 taaaagctaa aatggcaatc agccacggtt ggctaaaccc ggatctccaa aacggcattc
     2581 tcccttttag ccaacaacta acgacccacg gcttacttaa agggcatcaa attctccaac
     2641 aaacccagcg ggatttaacc gaaattaacg ccgtcaatcc ggaattgatg ttgctagatt
     2701 gcctgacgaa gttggttttg gtgtttgagt aaaagccctc tctcatagaa ggtagctttc
     2761 aattagccgt aaaaagaggc ttctagccct ataatatagt ttataacttg caaaattatc
     2821 tatctttgtt attgtatgaa taattcacac aaggagaata ttatggcaag aacaaccagc
     2881 gtgactttag gcgaaccttt aaatgatttt gtaaatgaaa tggtggcaag tgggcgttat
     2941 ggctctacca gtgaagtagt gagaaccgct ttacgaatgc ttgaggaaaa agagcaatac
     3001 ttagctcagt tgcgacaagc cattgatgat ggtgatgcta gtggttatgc aaccgaaacg
     3061 attgatgaaa tgatgttgag agtaaaggcg gaacttaatg tataaacttt ctgctcaagc
     3121 actccgagat caagagaaac tttttcgtta cagtatctct caatttggtg ttcaacaaac
     3181 agaatcttat tttgcagatc taaagcatat tttgaattta ttagctgatt ttccaacaat
     3241 gggaacttct gcggaacata ttcgtggtgg cttgcgaatg cacacacatc aatctcatac
     3301 aatttattat cgaataaaag cagatcatat atttattgtt cgcatcttac ataaccaaat
     3361 gaatcataaa cgctatattt agaaaaatga aagacttatt tatcatagac tcacattgtc
     3421 atctagattc tctagactac gaaactcgtc acaaaaacgt ggacgaagta attgaaaatg
     3481 ccaaagcccg aggggtgcat cattttatct cggtttgcac tacagttggg cgttttgaag
     3541 caatgaaaaa cttaaccgcc catcgtaatg acgtttcact ttcttgtggc gttcacccgt
     3601 tgaatgtgga agatgaacct tttgattacg ataaattatt tcagttcgct caagatccga
     3661 aagtggttgc cattggggaa actggtttgg attatcacta cacaccggaa accaaagcgt
     3721 tgcaacaatc gcttttcgtt caacaaattg aaattgcaaa caaactcaat aagccactca
     3781 ttatccattc tcgttcggca cgccaagata cgatggatat gttggaacaa aataatgccg
     3841 aaaaatgcgg tggggtgttg cattgcttta ccgaagattg ggcaatggca aaacgggctt
     3901 tagacatcgg cttttatatc tcaatttcgg gcattattac tttccgcaat gcagaagaac
     3961 tccgagaagt cgtgcgaaaa gtgccgttag atcgcttatt ggttgaaaca gattcccctt
     4021 atcttgcccc gattccttat cgtggtaagc cgaaccagcc ggcgtatgtg cgggaaactt
     4081 gcgaatatgt ggcgacatta aaaggcgttt caactgaaga attagctcgc attacaaccg
     4141 aaaatgtgca acacttattc aacattaaat tataaggaac taatatgaga gcaatactta
     4201 agtattttct gattcttgtc tcactatcat tttttattgt attaggcggt gcagttaatt
     4261 atgcaatgcc aagttatgaa gataccgtag taacaggtat ggaagtacgc cgaatggata
     4321 aagatggtat tatcagcaaa gcgaatcctg ctgatggcga ggtgcgcgat gtttatttct
     4381 tattcacgga agatcctgaa accaaaaagg taatggttta tcgtaatgaa gataccggct
     4441 gggggttgcc tccttatttc aaattcggat ctgccgacat tcaagccaaa gctcaagcct
     4501 acgctaacga aaaacaacgt gtgcaaatta aatattacgg ctggcgaatc aattggctaa
     4561 atgaatttag aaatattgtt tcaatcaagc cattagaaga agcagaaaca gtcagcaaac
     4621 caattatgag ctatgtactt tatgccgtat tggcgttctt gttcttcctc tcagtacaat
     4681 ttattcgtgg catttttaaa gattaatgcc gattaacctt tgacaaacgg ttcgattttg
     4741 taaaaaattg aaccgttttc ttttttatca acaatatcaa atgacgtact attttattgc
     4801 cgatctccac ttaaatgaaa atcagcctga aattacccac ctctttttgc aatttatgca
     4861 agaaaaagca ctacttgcag aagcggttta tatcttaggt gatctgtttg atttctggat
     4921 tggcgatgat gagcagtctg aactcatttc aaccgttaaa caggcaatca aaaccctcac
     4981 tacaagcggt acaaaatgct attttatttg cggaaatcgt gattttttaa ttggcaaacg
     5041 attcagcgaa caaacgggta tggttatttt gcctgattat cacgttatag atttattcgg
     5101 cacatctacc ttgctttgcc acggcgatac gctttgtact gatgatgtga aatatcaaca
     5161 atttcgtaaa aaagtccacc aaaaatggcg acaagctatc tttttgatgt ttccgctttc
     5221 ttggcgaatt cgtattgcgg agaaaattcg atccaaaagc aaacaagaaa agcgtgataa
     5281 atccgctgaa attatggacg taaacgctga agttaccgct caaattgtcg caaaatacaa
     5341 tacaactcaa cttatacacg ggcatacgca tcgacaagca atccatcagc ataatggatt
     5401 tacccgcatt gtattaggcg actggaaaga aaattacgcc tctattttaa gcgtaaccga
     5461 gcaaggagct gaatttatat gattaatgtg attattcctt gctataacgc cgaaaacacc
     5521 cttgttcgtg cagtgcaaag tgtattaaat cagtctggat taaatctatt gtggcttatt
     5581 gatgatgctt caacggataa tacgcttgcc ttagctaaac atttacaagc ccaagtgcct
     5641 gataaaatta gagtggaaca aatgcctcaa aatggcggtg ttgccaaagc acgcaactgg
     5701 ggagcattac aaagtgaaac agatttaatt gcctttttag atgctgatga tgcttatgaa
     5761 aacggggcat tgcaagttgc tgaaaagatt tttgagttta aaccggaaac cggcttggtt
     5821 cggttagcat taaaaccggt aaacttagat caacgttaca gctctcaccc taattttgag
     5881 ttagcttggc aatatatgca aatgactggt gcaggcaaca ctgtatttcg gcgtagtttc
     5941 ttcctcgctt gtggcggctt tccacaacat cagctcttcc aagaattagg tggagaagat
     6001 ggtgctttag gcatagccac tacacaaatt agctctgtag ctactgcatt taatgatgtc
     6061 ggtgttttac attactgccg agagggtatg cacgcagaaa gattattaaa tgcgatttta
     6121 ttcaacgaaa aagatcccaa tgttactgaa gaaaaagtta aacaagccaa cctaattacc
     6181 gaaaatattg ttaataatat tagaaatcta caagactgtc ttaatagtaa agggattggt
     6241 attaagcctt tgatatacgc ttgaatggag ctaaaaaagc atttgcagat tttccctaaa
     6301 aaaacaaccg cttgtattct tgagccttct ttcctctccg
//
LOCUS       DANKBV010000128         6307 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.128, whole genome shotgun sequence.
ACCESSION   DANKBV010000128 DANKBV010000000
VERSION     DANKBV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.128"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1046)
                     /gene="hisD"
                     /locus_tag="PWY43_002032"
     CDS             complement(<1..1046)
                     /gene="hisD"
                     /locus_tag="PWY43_002032"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDL2136886.1"
                     /translation="MQTLIWNNLTETEQQQYLARPAQVVGESIKAAVNEIKANVIANG
                     DKALFELAEKFDKVKLESLVVSEQQIQQAQSRISPELKQAIQNAKANIEAFHKAQQNQ
                     EVDIETQPGVCCQVITRPINAVGLYIPGGSAPLFSTVLMLAVPAKIAGCNKIVLCSPP
                     PIADEILYTAHLCGVETIYAVGGAQAIIAMANGTQTVAKVDKIFGPGNAFVTEAKRQV
                     VQEGTAIDMPAGPSEVLVIADEFADPEFVASDLLSQAEHGADSQVILVTPSESLAKAV
                     AEELEKQVSVLPRAETACKALAHCRTFIAENLQQAVEISNKYAPEHLIVQTEDARSLL
                     ADLDNAGSIFLGAYS"
     gene            complement(1058..1957)
                     /gene="hisG"
                     /locus_tag="PWY43_002033"
     CDS             complement(1058..1957)
                     /gene="hisG"
                     /locus_tag="PWY43_002033"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDL2136887.1"
                     /translation="MTTQNRLRIAMQKKGRLSQECSELLKQCGVKIIWNDQRLIAYSE
                     NMPIEILRVRDDDIPGLVFDGVVDLGIIGENVLEEVELGRLSAGENVAYKKLRQLDFG
                     GCRLSLAIDRDKNYNNIKDLEDAVIATSYPNLLKRYMKEQNVPFKSILLTGSVEVAPS
                     AGIANAICDLVSSGATLEANGLKEVEVIYRSTACLIQRQEPLSAEKQALVDKLLTRIQ
                     GVQQANESKYIMLHAPKDKLAEITALLPGVENPTILPLANDQTRVAMHVVSQENLFWE
                     TMEQLKEMGASSVLVLPIEKMLA"
     misc_feature    complement(1990..2117)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            2316..3173
                     /locus_tag="PWY43_002034"
     CDS             2316..3173
                     /locus_tag="PWY43_002034"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL2136888.1"
                     /translation="MQISLKKIYSGKVRDLYEIDEKRMLMVATDRLSAFDVILDDPIP
                     RKGEILTQISNFWFNKLAHIMPNHFTGDSVFDVLPQEEAEAIQHRAVVCKRLIPVKIE
                     SIVRGYLTGSGLKDYQQTGTICGLTLPEGLVEASKLPQPIFTPSSKAEVGDHDINISY
                     EECERQIGQELAAKVRDAALKLYTEAANYALTKGIIICDTKFEFGLDENGELTLMDEV
                     LTPDSSRFWSVETYREGTNPPSFDKQFVRDWLENSGWNKQPPAPKIPAEVIEKTVAKY
                     QEALDLLTK"
     gene            complement(3314..4795)
                     /gene="ilvC"
                     /locus_tag="PWY43_002035"
     CDS             complement(3314..4795)
                     /gene="ilvC"
                     /locus_tag="PWY43_002035"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDL2136889.1"
                     /translation="MANYFNTLNLRQKLDQLGRCRFMDRDEFADGCNFLKGKKIVIVG
                     CGAQGLNQGLNMRDSGLDIAYALRAEAIAEKRASFKRASENGFVVGTYEQLIPEADLV
                     INLTPDKQHSKVVADVMPLMKQGAAFGYSHGFNIVEEGEQIRPDLTVVMTAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGLAIAKAWASATGGDRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSIVCYDKLVSEGKDPAYAGKLIQYGWETITEALKQGGITLMMDR
                     LSNSAKLRAFELSEQIKEELAFLFEKHMDDIISGEFSSTMMADWANGDANLLKWREET
                     GKTAFENAPQADGIKISEQEYFDNGVLMVAMVKAGVELAFDTMVSAGIYEESAYYESL
                     HELPLIANTIARKRLYEMNVVISDTAEYGNYLFSHVATPILAEKIIPTLEKGDLGEPT
                     PAVEIDNVTLRDVNDAIRNHPIELIGQELRGYMTDMKRISSQG"
     gene            5017..6261
                     /locus_tag="PWY43_002036"
     CDS             5017..6261
                     /locus_tag="PWY43_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HDL2136890.1"
                     /translation="MKINKIILQSCGECGQQVRVPLMLNGNAVCPVCHNILRKNNTWS
                     LRRSAFLALAILILLPFALHFPLMSIDLLGIKINATVWDGIWKMATEGYPYTAFMVLV
                     CAVIMPIAFVLLILTVQLQRIIRQRPRYTLIFLSKIKEWVMLDVYLVALAVAAFKIRD
                     YADLQFDVNLIAFVLVTILNTLLFIKTDPKQAWERFYPEYHALPFDHPSNPTLCPTCE
                     YCFDETILDLKGQQRCPRCESNLSISDNVKIQRVWACLIAGTIMMIPANIFPISTTEL
                     AGQVSADSLMSGVLLFMEMGSYTVAAIVFIASIAVPVSKITIIFYLLLAIHYKWKHPI
                     HWQMKLLHYVHFVGRWSMLDLFVLSLMMSLLERGQILSFSVGEAAFYFGAAVFLTMFA
                     SANLDARMLWKIHYDHKRKATH"
BASE COUNT         1758 a         1461 c         1218 g         1870 t
ORIGIN      
        1 gaatacgccc ctaagaaaat cgagccggca ttatctaaat ccgccaacaa cgagcgggca
       61 tcttcagttt gcacaattaa atgctcaggt gcatatttat tgctaatttc gaccgcttgt
      121 tgcaggtttt ctgcaataaa agtacgacag tgagccaatg ctttacaggc agtttcagca
      181 cgaggcagaa ccgacacctg cttttctaat tcttcagcga ctgcttttgc caagctctca
      241 cttggtgtga ctaaaattac ttgggaatcg gccccgtgtt ccgcttgaga gagcaagtcg
      301 ctcgccacaa attcagggtc ggcaaactca tcggcaatca ccaacacttc cgacgggcct
      361 gccggcatat caatcgccgt gccttcttgc acaacttggc gtttcgcctc cgtcacaaac
      421 gcattgcccg ggccgaaaat tttatccact ttcgctaccg tttgtgtgcc atttgccatc
      481 gcaataatcg cttgagcccc cccaactgcg taaatcgttt ccaccccaca caaatgggcg
      541 gtgtagagaa tttcatcggc aatcggcggt ggtgagcaaa gcacaatctt gttacaaccg
      601 gcaatttttg ccggcactgc aagcattaac acggtagaga aaagcggggc tgaaccgccc
      661 ggaatataaa gccccaccgc attaatcggg cgagtgatga cttggcaaca cacccccggc
      721 tgggtttcaa tatccacctc ttgattttgc tgtgccttgt ggaaagcctc gatattcgct
      781 ttcgcatttt gaatcgcttg cttaagctct ggtgaaatac ggctctgtgc ttgctgaatc
      841 tgctgttctg atacaactaa gctctctaat tttactttat cgaacttttc agccaattca
      901 aataatgcct tatcgccgtt ggcaattaca ttcgctttaa tttcattaac tgccgcttta
      961 atactctcgc caaccacctg tgccggacgg gcaagatatt gctgctgctc agtttctgtt
     1021 aaattgttcc aaattagggt ttgcatattt attcctttta cgccaacatt ttctcaatcg
     1081 gtaaaaccaa tacggaactt gcgcccattt cttttagctg ctccattgtt tcccagaaca
     1141 ggttttcttg gctgacaacg tgcattgcca cacgggtttg gtcgttagct aacggtaaaa
     1201 tagtcgggtt ttccacaccg ggtaataaag cggtaatttc cgccagtttg tctttggggg
     1261 cgtgcagcat aatatactta gattcgttcg cctgttgcac accttgaatg cgggttaata
     1321 atttatccac taacgcttgt ttttccgctg aaagtggttc ttggcgttga attaggcaag
     1381 cagttgaacg gtaaatcact tcaacctctt ttaatccgtt cgcctctaat gttgcacctg
     1441 aagaaaccaa gtcacaaatc gcattcgcaa ttccggcact tggagcaact tcaaccgaac
     1501 cggtgagtaa aatactttta aacggcacat tttgctcttt catataccgc tttaataaat
     1561 tggggtaaga agtggcaatc acagcatctt ctaaatcttt gatattgtta taatttttat
     1621 cacgatcaat agctaacgat aaacggcagc caccaaaatc taactgacga agttttttat
     1681 aggcaacatt ctcaccagca cttaatcgcc ctaattccac ttcctctaat acattttcac
     1741 cgataatccc aagatcaact acgccgtcaa aaactaagcc tggaatgtcg tcatcacgca
     1801 cccgtaaaat ttcaatcggc atattttctg aataggcaat caggcgttga tcgttccaaa
     1861 tgattttcac gccacattgt ttaagcaact cactacattc ttggcttaag cgaccttttt
     1921 tctgcatcgc aattcttaat cgattttgtg ttgtcattgt tgtttccttc ttatgtttta
     1981 aattgtaaaa ataaaaaaac ctctcgaaat cacttccgag agggttaaat ttatgctgcg
     2041 tttaggcgta ctcggaagtc ttatcttccg cacataccaa atgcccgaaa gattattcag
     2101 gtaaatgatg gtggtgatga gtacgaataa aagccatttt gttgttctcc aaactaaatt
     2161 gctttaaaag atacgcttaa attttggcaa tgacaagtgt tttttgcgaa aaaattgcta
     2221 tttttcatct ctctaaaaca actatcgcaa acgtttgcgt aaagcggtat aatttgcctg
     2281 attttttgca aattcaacaa agtaggaata acactatgca aatcagcctc aaaaagatct
     2341 actccggcaa agtccgtgat ctctacgaaa tcgatgaaaa acgtatgctt atggttgcca
     2401 ccgaccgatt atctgccttt gatgttattt tagacgatcc tattcctcgt aaaggcgaaa
     2461 tcctcaccca aatctccaac ttctggttca acaaattagc ccacattatg ccaaaccact
     2521 tcaccggcga cagtgtgttt gacgtgttac cgcaagaaga ggcagaagct attcaacatc
     2581 gtgcggtggt ttgcaaacgc ttaataccgg tgaaaattga atcgattgtg cggggttatt
     2641 taaccggctc cgggctgaaa gattaccaac aaactggcac gatctgcggc ttaacattgc
     2701 cggaaggctt ggttgaggcc agcaaattac cgcaaccgat cttcacaccg tccagcaaag
     2761 ccgaggtggg cgatcacgat attaatatca gctacgaaga gtgtgaacgc cagatcggtc
     2821 aagaacttgc cgccaaagtg cgtgatgccg ctctaaaact ctacactgaa gcggcaaact
     2881 atgccctgac caaaggaatt attatttgcg acaccaaatt cgaattcggc ttagacgaaa
     2941 acggtgaact aacgctaatg gacgaagtcc taaccccgga ttccagccgt ttttggtcag
     3001 ttgaaaccta ccgagaaggc acaaatccac cttcatttga taaacaattc gtgcgtgact
     3061 ggctggaaaa tagcggttgg aacaaacaac ccccagcccc gaaaataccg gcagaggtaa
     3121 ttgaaaaaac cgtagcgaaa tatcaagaag cattggattt attaaccaaa taacaccaca
     3181 agcggtcaaa tttacgctat tttttgcaaa acaaaaacct gtaagccaac acttacaggt
     3241 tttttagtta gtggtaaaaa gtgcggttat tctaccgcac ttttgcaaaa ttttgtgtaa
     3301 atctgaccgc ttgttagcct tgcgaagaaa tacgtttcat atctgtcata taaccacgta
     3361 attcttgacc gattaactcg attgggtggt tacggattgc gtcattcaca tcacgtaagg
     3421 taacgttgtc gatttccact gccggagttg gttcacctaa atcgcctttc tctaacgttg
     3481 ggataatttt ctccgctaaa atcggggttg caacgtgaga gaataagtag ttaccgtatt
     3541 ctgcggtatc tgaaattacc acgttcattt catataaacg cttacgagcg attgtatttg
     3601 cgattaacgg taactcgtgt agagactcgt agtaagccga ttcttcataa ataccggctg
     3661 aaaccattgt atcaaaggct aattcaaccc ctgctttcac cattgcaacc attaacacac
     3721 cgttatcaaa gtattcttgc tcagaaattt taataccgtc tgcttgtggt gcgttttcaa
     3781 aggctgtttt acctgtttct tcacgccatt ttaataagtt ggcatcgccg tttgcccagt
     3841 ctgccatcat agttgatgag aactcaccac tgatgatgtc gtccatatgt ttttcaaata
     3901 agaacgctaa ttcttctttg atttgctcag aaagctcgaa cgcacgcaat ttggcactgt
     3961 ttgataagcg atccatcatt aaggtgatac cgccttgttt taacgcttcg gtaatggttt
     4021 cccaaccgta ttggatcaat ttacctgcgt aagccggatc tttaccttcc gacactaatt
     4081 tgtcgtagca cacgattgaa cctgcttgta acataccgca aagaatggtt tgttcaccca
     4141 ttaaatcaga tttcacttct gctacgaatg aagattctaa tacgcccgca cggtcaccac
     4201 cggtagcact tgcccacgct ttcgcaatgg ctaaaccttc gcctttaggg tcattttccg
     4261 ggtgaacggc aattaaggtt ggcacaccga aaccacgttt gtactcttca cgcacttccg
     4321 tgcccgggca tttcggagcg gtcatcacaa ccgttaaatc cggacgaatt tgctcacctt
     4381 cttccacaat gttgaaaccg tgtgaataac caaatgccgc accttgtttc attaacggca
     4441 ttacatcggc aactacttta gagtgctgtt tgtccggcgt taagttaatc actaaatccg
     4501 cttcaggaat taattgctcg taagtaccca ccacaaagcc gttttcagaa gcacgtttaa
     4561 atgaagcacg tttttcagca atcgcttcgg cacgtaaagc gtaagcaata tctaagcctg
     4621 aatcacgcat atttaaacct tggtttaaac cttgagcacc acaacccaca atcacgattt
     4681 ttttgccttt taagaaattg cagccatctg caaattcatc acgatccata aagcggcaac
     4741 gacctaactg gtctaatttt tgacgtaaat ttaaagtgtt gaaatagtta gccatttttt
     4801 aatccttatt tgaattaatg agattgatat gatgttttgt ttgcacattg ttatagtttg
     4861 atttgattat accttaacct aatattgcgt aaattgaaaa tattaatatt tcatattgca
     4921 taaaatgcaa cttgcaaaaa ttttgccaaa tttaaccgct tgcaatctct acgataaagt
     4981 agaaaataac cacctaattt aatagaagat caaaaaatga aaataaacaa aatcattcta
     5041 caaagctgtg gcgaatgtgg gcaacaggta cgagtgccgc ttatgctcaa tggaaatgcc
     5101 gtttgcccgg tatgccataa catattacgc aaaaacaaca cttggagctt acgccgcagt
     5161 gcttttttgg cgttagcaat tttgattttg ctcccctttg cacttcattt ccctctaatg
     5221 agcattgact tactcggcat caaaattaac gctaccgtat gggacggcat ttggaagatg
     5281 gcaaccgaag gctaccctta caccgctttt atggtgctag tctgtgcggt aataatgccg
     5341 attgcatttg tattgctaat tttaaccgtt cagctacaac gtattattcg ccaacgcccc
     5401 cgttataccc tgattttctt aagcaaaatc aaagaatggg taatgcttga tgtctattta
     5461 gttgcccttg cagttgcagc ttttaaaatt cgagattatg ccgatctaca atttgacgta
     5521 aatttaatcg cctttgtgtt ggtaaccatc ttaaacaccc tactttttat taaaaccgat
     5581 cccaaacaag cgtgggaacg tttttaccca gaatatcacg ccttaccgtt tgatcaccca
     5641 agcaatccaa cactttgccc gacctgtgaa tattgctttg atgaaaccat cttggattta
     5701 aaaggacaac aacgctgccc tcgttgtgaa agcaatttat caatttccga taacgttaaa
     5761 atacaacggg tttgggcttg cttaattgca ggaacgatta tgatgattcc ggcaaatatt
     5821 ttcccgattt cgaccactga attggcaggg caggtttcgg cagattcatt aatgtccggc
     5881 gtgcttctgt ttatggaaat gggtagctac accgtggcag cgattgtgtt tatcgccagt
     5941 attgccgtgc cggtgagtaa aattaccatt attttttatc tcttgctggc aattcattac
     6001 aaatggaagc acccgattca ttggcaaatg aaactactgc actacgtcca ttttgtcgga
     6061 cgctggtcaa tgctggattt attcgtgtta tcattgatga tgtcgctact cgaacgaggg
     6121 cagattctca gtttctcggt aggggaagcc gctttctatt ttggtgcagc cgtattctta
     6181 acgatgtttg cctctgcaaa tttagatgcc agaatgctgt ggaagattca ttacgatcat
     6241 aaacgcaaag ctacccacta aattcccttt atcgttaaaa acataaaata gcttaggacg
     6301 ggcttct
//
LOCUS       DANKBV010000129         6261 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.129, whole genome shotgun sequence.
ACCESSION   DANKBV010000129 DANKBV010000000
VERSION     DANKBV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.129"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..241
                     /locus_tag="PWY43_002037"
     CDS             <1..241
                     /locus_tag="PWY43_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2136891.1"
                     /translation="IKDQQVVLGLITSKFGDLEDKNAIIERIKEAAQIVPINQLCLSP
                     QCGFASTEEGNILTEAAQWAKLNLIKEIAEEVWGS"
     gene            complement(298..1686)
                     /locus_tag="PWY43_002038"
                     /pseudo
     CDS             complement(298..1686)
                     /locus_tag="PWY43_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
     gene            complement(1846..2745)
                     /gene="cdd"
                     /locus_tag="PWY43_002039"
     CDS             complement(1846..2745)
                     /gene="cdd"
                     /locus_tag="PWY43_002039"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL2136892.1"
                     /translation="MFRTDITERFEQLKAEKNCAVTQAIIEQLIKQDFHGQPSYEVVD
                     ELCEKFKRSRVELALYCIAIAACYAVTPVSDFNVGAVAIGKNGDFYFGANQEFSGECM
                     QQSVHAEQSAISHAFLAGETLITDVVVNYTPCGHCRQFMNELNSAASLKVHLPHSQNN
                     LLHSYLPDSFGPKDLGIEKVLFDEQPQQFEVKGDHLQQAAIFAANNAYAPYSNAFSGV
                     ALQVGERIVCGKYTENAAFNPTFLPLQSALNYRLFLGLADIPVTRVVLAEAKGSLSSY
                     AMTQALAKSLFGLEIEYLSLQAV"
     gene            complement(2759..3661)
                     /locus_tag="PWY43_002040"
     CDS             complement(2759..3661)
                     /locus_tag="PWY43_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL2136893.1"
                     /translation="MISSSLKLGYFSLIVATLFWGGNFVLGKVLSGAVQPITLTYLRW
                     LPAFLILVLLFFRPTWRVMHLLKPRLGVMWVLGLLGVILFPATLYEGLKTTTALNASL
                     YLAISPVLVLFLNRLVFKEKISPIILSGAIISLLGVLWLLMQGKLTRLFSLAINQGDL
                     WAIASTISWAVYCCVIRLRPQGISNTVFLTVLVGLAVLTLTPFFIYEYVAESAKNLAN
                     LTALQWSGVGYLVIGPSILSYAFWNFGIAIVGSAKGAVMTNFTPLFAAIFSIILLNES
                     VHSYHIISAILITAGILICSYKKH"
     gene            complement(3658..4014)
                     /gene="folB"
                     /locus_tag="PWY43_002041"
     CDS             complement(3658..4014)
                     /gene="folB"
                     /locus_tag="PWY43_002041"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDL2136894.1"
                     /translation="MSDKVFIRELTAFASIGAYNWEHTIKQRLVFNIDMAWDFSKAAE
                     TDDVQYCLNYAEVSQKVLDFVESQPFKLVETVAYQLAELLQKEYQLSWIRIELHKPKA
                     VAQASSVGVIVERGHQ"
     gene            4084..4689
                     /gene="plsY"
                     /locus_tag="PWY43_002042"
     CDS             4084..4689
                     /gene="plsY"
                     /locus_tag="PWY43_002042"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL2136895.1"
                     /translation="MNLIPYLLILTAYLLGSISSAVIFCKLAGLPDPREVGSHNAGAT
                     NVLRIGGKSAALGVLLFDILKGTLPVAMGIYFELSPSQLGFIALAVCLGHIFPIFFQF
                     RGGKGVATAFGAISALSFIVAGGAIGTWLLVFLISGYSSLSAVFTALVVPFYIWWFEP
                     QFTFPVALVCCLLVYRHHDNIQRLWRGQEDKLWDKFKKNRA"
     gene            complement(4763..>6261)
                     /gene="parE"
                     /locus_tag="PWY43_002043"
     CDS             complement(4763..>6261)
                     /gene="parE"
                     /locus_tag="PWY43_002043"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2136896.1"
                     /translation="YSIAFANGVKVEDLTVIGTCPKKQTGTTVRFYPNPKYFDSPRFS
                     VSRLRHLLRAKAVLCPKLTINFIDKINNTEESWYYEDGLSDYLSEALNGYETVPNPPF
                     IGDVTAETEAVSWALTWLPEGGELVAESYVNLIPTAQGGTHVNGLRNGLLKAMVEFCE
                     IHNLLPKGVKLTADDVWNRCAYVLSLKIQEPQFAGQTKERLSSRQASSYVDSTLKDAF
                     SLWLNQNVQTGKLIAEMAISSAQSRMRAAKKVVRKKLVSGPALPGKLADCTAQDLSRT
                     ELFLVEGDSAGGSAKQARDKEYQAILPLRGKILNTWEVSSDQVLASQEVHDIAVALGI
                     DPDSDNLEELRYGKVCILADADSDGLHIATLLCALFLRHFPKLVQNGHVYVAMPPLYR
                     IDIGKDEVHYALDEAEKEAILARLAKKKGKPNVQRFKGLGEMNPSQLRETTMDPSTRR
                     LVQLTFDDTAEEGELNTFEIMDMLLGKKRAEDRKQWLQNRGDEADLAV"
BASE COUNT         1972 a         1422 c         1130 g         1737 t
ORIGIN      
        1 cattaaagat caacaagtgg tactaggctt aattacctct aaatttggcg atttagaaga
       61 taaaaatgcg atcattgagc gtattaaaga agcggctcaa attgtgccaa tcaatcagct
      121 atgcttatca ccacaatgcg gctttgcctc caccgaagaa ggcaatattt taaccgaagc
      181 cgcacaatgg gcgaaactaa acttaattaa agaaattgcg gaagaagttt ggggtagcta
      241 atcctctata atacaaatag ccgagtaaat actcggctat tttagtttgt tttaacatta
      301 caaccagaag agatacccaa cagtcgcaat cacaaagata gagataatat ttagccagaa
      361 accaacttta atcatctcac tttgtttaat ttgccctgta ccaaatacaa tcgcattcgg
      421 tggggtggca accggcaaca taaaggcgca agatgctcct agaccaataa ttaaggctaa
      481 tccgaccggt ggcatattta atgcttgtgc tatagagatg aaaatcggca ctaacaaagc
      541 cgcactggct gtgttagagg taacctcagt taggaaaata ataaatgctg ccacaattaa
      601 accaattagg taaaaatgcc ccccttcaat tagtgagacg accccatcag ccataatttt
      661 gctcgcacca gtttgtccta atacagcact taacgtcaaa ccaccaccaa ataggaataa
      721 taccccccac tcggtgtttt cttgaatctg ttgccaattt gcgacacgag taatgcaaat
      781 aagtgctgcg gcaatcaatg caagaatggt gtcaaagctc gcaattttgc cattaattcc
      841 cagaaatgca gaaataatag ggttaatttt atcaccaaaa atccaaccaa gcgcaataaa
      901 gacgaaaata gccaaggtga taatacgaga acgatttaat tcaattttct caaatgatcg
      961 atcaaaacta actgaaaaat ttggtttaaa tacgatatat agtgttgcaa tcattaatgg
     1021 gaataaaatt agcataattg gcaaaccata ttttaaccaa tcagcaaaag tcagattcaa
     1081 ttgagaagca acaatcgcat tcggtgggct tcccacgaaa gtacccattc caccaatact
     1141 ggcactataa gcaattccta ataacacgaa aagataagta ttgtggttct gtttctgatc
     1201 aagtttactt aaaatcccca ttgccaaagg taacatcatt gcagctgtag ctgtattact
     1261 catccacatt gacaaaaacg ctgttaccgc aaataaaata actgcaatcg ataatttacc
     1321 tttagctaat gccataattt tattggcaat catacgatct agctactgaa tgtgtaatgc
     1381 tgtcgctaag gcaaagcctc caaaaaataa gaaaatcgtt ggatcagcaa aagcaacaag
     1441 agcttctttt gatttaacca gccccagtgc tacagccaaa atcggaatta ataatgccgt
     1501 aaccgtaacg tgtaatgctt ctgttagcca taagacggca atgaaaatta acaatgccaa
     1561 ccccttattg gcttcaggcg taaaaggcaa ggtatttaac aaaataataa ataaaatagc
     1621 atctgcaaca aaaatcaagc tattgcgtgt atttttactc ggttcaataa gaggagtatt
     1681 ttccataaaa acctcacaaa atatagataa tcagtggtat cactatatat aaaaaaacga
     1741 tataggcaaa tataattgtt gcaaaagtgt gaagctcatc aaaaaattta acactaaaaa
     1801 ataaattgct taaagataca gcacctttgc aaaaataaat caaaattaca ccgcttgtag
     1861 cgaaaggtat tcaatctcta acccaaatag tgattttgcc aatgcttgag tcattgcata
     1921 actactgaga gagcctttcg cctcagccag cacaactcga gtaacgggaa tatcggcaag
     1981 ccctaaaaat aatcgatagt tcaacgcgct ttgcaacggt aaaaaagtag gattaaaagc
     2041 tgcattctca gtatatttac cacaaacaat acgctcacct acttgcagtg caaccccact
     2101 aaatgcgttg ctgtaagggg cataagcatt attggcagca aaaattgccg cttgttgtaa
     2161 atgatcgccc ttaacctcaa attgttgcgg ttgttcatca aataagactt tctcaatccc
     2221 taaatctttc ggtccaaaac tatccggcaa atagctatgg agcaaattat tttggctatg
     2281 cggtaaatgt acttttaagc ttgccgcact gtttaattca ttcataaatt ggcggcaatg
     2341 cccacaaggg gtatagttca caaccacatc tgttatcaaa gtttcccctg ctaaaaatgc
     2401 gtgcgagatt gcactttgct ctgcgtgaac ggattgttgc atacattccc cggaaaactc
     2461 ttggtttgcc ccaaaataga aatctccgtt tttgccaatc gcaaccgccc ctacattaaa
     2521 atcggaaact ggtgtaaccg cataacaggc agcaatggca atgcaatata aagccaactc
     2581 aacacgagag cgtttaaatt tttcacagag ttcatcaacc acttcataag agggctgccc
     2641 gtgaaaatct tgtttgataa gttgctcaat aatagcctgt gtaactgcac agtttttctc
     2701 tgcttttaat tgttcaaaac gctctgtaat gtctgttcga aacatacggt acctccgttt
     2761 aatgtttttt atagctgcaa atcagtatcc ctgccgtaat cagtatggca ctaataatat
     2821 ggtaagaatg aactgattca tttaataaaa taatactgaa tattgctgca aagagcggcg
     2881 taaaattagt cattaccgct cctttagcag aaccgactat tgcaatgccg aaattccaaa
     2941 acgcataaga gagaatagaa gggccgataa ccaaatagcc taccccactc cattgtaaag
     3001 cggtcaaatt tgctaaattt tttgcagatt ctgctacata ttcatagata aaaaatggcg
     3061 ttaacgttag aactgccagc cccactaata cggtcaaaaa caccgtattg cttatacctt
     3121 gtggacgtag ccgaatcaca caacaataaa ccgcccagct tatagtagaa gcaatcgccc
     3181 ataaatcacc ttgattaatt gccaagctga ataagcgagt tagcttccct tgcatcaata
     3241 gccacagcac tcccagcaaa ctgattattg ccccacttag aataatcggg ctgattttct
     3301 ccttaaaaac gagccgattt aaaaatagca ccaatacagg cgaaatagct aaatagagac
     3361 tcgcattcaa tgctgttgtc gtttttaaac cttcatataa ggtagccggg aataaaatta
     3421 ccccaagcag acctaatacc cacatcacac ctaaacgagg ttttaataag tgcattaccc
     3481 gccaagttgg gcgaaaaaag agcaaaacta aaattaaaaa agccggcaac caacgcaaat
     3541 aggttaaggt aataggctga actgccccac ttaacacttt gcctaataca aaattcccac
     3601 cccaaaaaag agtagcaacg attaaactga aataccccaa tttcagtgat gatgagatca
     3661 ttgatgacct cgttctacaa tcacacctac actgcttgct tgggcaaccg ctttcggctt
     3721 atgcagttca atccgaatcc aagaaagttg atactctttt tgtaatagtt ccgccagttg
     3781 ataggcaaca gtttcaacta atttgaaagg ttgagattca acaaaatcca gcactttttg
     3841 cgatacttcc gcataattca agcaatattg tacatcatcg gtttctgccg ctttggaaaa
     3901 atcccacgcc atatcaatgt taaaaaccaa gcgttgctta atcgtatgct cccaattgta
     3961 agccccaatg gaagcaaaag cggttagttc tcgaataaaa accttatcac tcatttttcg
     4021 tcctgtttta ttttctgaat ttctgtaaaa tatagcacaa gataaattta atcaggaaaa
     4081 tttatgaatt taattcccta tttattgatc ttaaccgcct atttgctcgg ctcgatttcc
     4141 agtgcggtca ttttttgtaa gctggcaggc ttgcctgacc caagagaagt cggctcacac
     4201 aatgccggtg caacgaacgt actacgaatt ggcggtaaaa gtgccgcatt aggtgtgctt
     4261 ttattcgata ttttaaaagg cacattaccg gtggcgatgg ggatttattt tgaactctct
     4321 ccctcacaac tcggtttcat cgccttagcg gtttgtcttg ggcatatttt cccgattttc
     4381 ttccaattcc gtggcggaaa aggcgttgcc accgcatttg gtgcaatctc cgcattaagc
     4441 tttattgttg caggcggtgc tatcggcact tggctactgg tgtttttaat ctccggctat
     4501 tcttctttaa gtgccgtctt cactgccttg gtcgtaccgt tttacatttg gtggttcgaa
     4561 ccacaattta ctttccccgt cgccttagtt tgttgcctat tggtttaccg ccatcacgat
     4621 aatattcaac gcttatggcg tgggcaggaa gacaaactgt gggataaatt caagaaaaat
     4681 agagcataaa aataggcata gtgaaccgtt cgctatgcct atttgcaaat tattgcggaa
     4741 aaatcaccgc ttgtcttctc gactaaaccg ctaaatcagc ctcatcgcct cgattttgta
     4801 gccattgttt acgatcttcc gctcgttttt tacccaataa catatccata atttcaaagg
     4861 tattcaattc accctcttct gccgtatcat caaaggttaa ttgcactaag cggcgagtgc
     4921 ttggatccat cgtcgtttca cgcaactgac tcggattcat ttcgcctaag cctttaaagc
     4981 gttgcacatt tggcttgcct tttttcttgg caaggcgagc taaaattgcc tctttttccg
     5041 cctcatctaa agcataatgc acctcatctt tacctatatc aatacgataa agtggcggca
     5101 ttgccacata aacgtgtccg ttttgcacca atttcgggaa atgacgaagg aataacgcac
     5161 acagcaaggt agcaatatgc aaaccgtcag aatccgcatc ggcaagaata caaactttgc
     5221 cataacgcaa ctcttctaaa ttatcgctat cagggtcaat tcccaaggct accgcaatat
     5281 cgtgtacttc ttgtgaagcc aatacttggt ctgatgaaac ttcccaagtg tttaaaatct
     5341 taccacgcag cggcaaaatg gcttgatact ctttatctcg agcttgtttt gccgaaccgc
     5401 cggcagaatc accttctacc aagaaaagtt cggtacggct gagatcttga gcagtacaat
     5461 ccgccagttt gcccggtaaa gccggaccac tcaccaattt cttacgcacc acttttttcg
     5521 ccgcacgcat acggctctgg gcggaactaa ttgccatttc agcaattaat ttacctgtct
     5581 gcacattttg gttaagccat aaactaaagg catctttcaa ggtgctatcc acatagcttg
     5641 aggcttgacg ggaagacaaa cgctctttgg tctgccctgc aaattgtggc tcttgaattt
     5701 tcagagaaag cacataagca caacggttcc acacatcatc agcggttaat tttacccctt
     5761 tcggcaataa attatggatt tcgcaaaact ccaccatcgc ctttaataaa ccgttacgca
     5821 agccattaac gtgcgtgccg ccttgtgcgg tcggaatcag gttcacatag ctttccgcta
     5881 ctaactcgcc cccttccggc aaccaagtta atgcccagct taccgcctct gtttcagcag
     5941 taacatcacc aataaacggt ggattcggca cggtttcata accgtttaat gcctcgctca
     6001 aataatcaga taagccgtct tcgtaatacc aactttcttc ggtattgttg attttatcaa
     6061 taaaattgat ggtgagtttc gggcaaagca ccgctttggc tcgtaacaaa tggcgtaaac
     6121 gactcaccga aaaacgaggg ctatcaaaat attttgggtt cgggtaaaaa cgcactgtcg
     6181 tgccggtctg ttttttcggg caagtaccaa ttaccgtcag atcttccact tttaccccgt
     6241 tggcaaaggc aatcgaatag a
//
LOCUS       DANKBV010000130         6258 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.130, whole genome shotgun sequence.
ACCESSION   DANKBV010000130 DANKBV010000000
VERSION     DANKBV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.130"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            15..599
                     /locus_tag="PWY43_002044"
     CDS             15..599
                     /locus_tag="PWY43_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HDL2136897.1"
                     /translation="MLKKLLIIDNHDSFTFNLVDLLRKLDVPAKVVLVEELDLNEVEH
                     FSHILISPGPDVPRAYLQTFAMLERYHQTKSILGVCLGHQTICEFFGGKLYNLNDVRH
                     GQQRTLTQIEPNPIFKGLPKQLKVGLYHSWGILQNSLQNTPLVPTAVCDQNVLMAFKH
                     QHLPIFGVQFHPESFITEFGQQMLANWLEITQDF"
     gene            696..1778
                     /gene="prfA"
                     /locus_tag="PWY43_002045"
     CDS             696..1778
                     /gene="prfA"
                     /locus_tag="PWY43_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL2136898.1"
                     /translation="MKDSIINKLESLSERHEELQALLGDASIISDQDKFRAYSKEYSQ
                     LEEVVGTFNRWKKLNNDIEEAQLLLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLP
                     KDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYK
                     EIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEIN
                     PSDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNKAKALAVLAS
                     RIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMN
                     GKIDELIQPIITEYQADQLAALSDQN"
     gene            1866..3119
                     /gene="prmC"
                     /locus_tag="PWY43_002046"
     CDS             1866..3119
                     /gene="prmC"
                     /locus_tag="PWY43_002046"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2136899.1"
                     /translation="MQTDNVTLSEWQFGEKPNTLANLVLAGIKTATCSGYCFYKLDNE
                     PLPQKGERHIVLDSQNCPVVMIELTCIERKKWFEIDEKFALLEGADSFSEWERTHRNF
                     FNAQLERAIDEQFELVCEQFHTVNIFSKTYAQWFRFAEQTLLENAEQDPFLNAKFDAN
                     LLLQAVTKRSKSAIFAFSETVLSERELVELAEKLARRAKGEPMAYILGYREFWSLPLK
                     VSPATLIPRPDTERLVELALEYANKRLENQKMLQILDLGTGTGAIALALASELGEKAQ
                     IIGVDFQNEAVQLAEENRQHLGLNQVTFLQSDWFSSLANQQFDLIVSNPPYIDEADEN
                     LTVGDVRFEPLTALVADNKGLSDLQKIIQNAPLYLKPQGALMLEHSWQQGNAVRDLFD
                     LNLWEQVETAQDYAGNDRVTKAILK"
     gene            3128..3994
                     /locus_tag="PWY43_002047"
     CDS             3128..3994
                     /locus_tag="PWY43_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HDL2136900.1"
                     /translation="MDDIIEELLKELKDPKVTISETELKKFLYREILRLTTLIDRNVS
                     EQQVFGQMSALVKKARYNVNGEGDEERINQLLDLVYREWGFHCYYEDYFHTENLLLNQ
                     VIRKRRGMPVSLGAVVLYLAAVLDLPIYPVNFPTQLVLRAEMRQPNGATKVRFINPWN
                     GEFLTIEMLNKWLEGEMGFGVEVTPDLLRRAEPQELLERVETVFKMALTREGKYEETL
                     RLIEYRLAFSPEDPYEIRDRGMVLASMDCYQAALEDINYFIDQCPEDPSAEILKMEVK
                     GLQQKSQESLVH"
     gene            4085..4939
                     /gene="kdsA"
                     /locus_tag="PWY43_002048"
     CDS             4085..4939
                     /gene="kdsA"
                     /locus_tag="PWY43_002048"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDL2136901.1"
                     /translation="MTDKIVKVGNIEVANHKPFTLFGGMNVLESRDMALRVCEQYVEV
                     TQKLNVPYVFKASFDKANRSSIHSYRGPGMEEGLKIFQELKQTFGVNIITDVHEIYQC
                     KPVAEVVDVIQLPAFLARQTDLVEAMARTGAVINVKKPQFLSPGQMGNIVEKIAECGN
                     DKVILCDRGTNFGYDNLVVDMLGFGIMKKVSKGCPVIFDVTHSLQCRDPFGAASGGRR
                     DQVTELARSGMAIGVAGLFLEAHPDPNSAKCDGPSALPLSKLEAFVSQMKAIDELVKS
                     FEEIDTSK"
     gene            5162..6109
                     /locus_tag="PWY43_002049"
     CDS             5162..6109
                     /locus_tag="PWY43_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDL2136902.1"
                     /translation="MLNIVFLDRTGIPATHEIPRPNFPHTWTEYATTSADQTFERVKE
                     ADIIITSKVLLQRDLLAKLPKLKLIAITATGTNNVDLAAAKELGIAVKNVTGYSTVTV
                     PEHVIGMIYSLKHRLADYHRDLITSDRWATCGQFCYVDYPIHDVRGSTLGIFGKGNIG
                     AEVARLAQAVGMQVIFAEHQGATEIREGYTAFEEVFKQADIISLHCPLTEQTQNLINA
                     ETLALMKPTAYIINTGRGPLIDEQALLEALEQGKIAGAALDVLVKEPPEKDNPLMLAA
                     KRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVETGK"
BASE COUNT         1883 a         1246 c         1460 g         1669 t
ORIGIN      
        1 aagaaaataa taaaatgcta aaaaaactat taatcatcga caaccacgac tcttttacct
       61 tcaacttggt ggatttactc cgtaagcttg atgtgccggc gaaagtggtg ctggttgaag
      121 agcttgacct caatgaggtg gagcatttta gccatatttt gatttcgccc ggccctgatg
      181 tgccgagagc ctatctacaa acctttgcga tgttggagcg ttatcatcaa accaaatcaa
      241 ttctaggcgt ttgcttgggg catcaaacga tttgcgagtt ttttggcggc aagttgtata
      301 acctaaacga tgttagacac gggcagcaac gcacgctcac tcaaattgag cctaacccca
      361 tatttaaagg tttgcctaag cagttgaaag ttgggttgta tcactcttgg ggcattttgc
      421 aaaattcgtt gcaaaataca ccgcttgtac caaccgcagt ttgcgatcaa aatgtgttaa
      481 tggcgtttaa acaccaacat ttgccgattt ttggcgtgca gtttcacccg gaatctttta
      541 tcactgaatt tggtcaacaa atgttggcaa attggcttga gattacacaa gatttttagc
      601 gtgaaatccg ctataatccg tcagttattt acccttttaa aatctgtagg ggcgaaaaat
      661 ttttcgcctt acttaatatg caagagataa tttaaatgaa agattcgatt atcaataaat
      721 tagaaagttt aagtgaacgc cacgaagaac tacaggcgtt acttggcgat gcctcaatta
      781 tttccgatca agacaaattc cgtgcttatt ccaaagaata ttcgcaatta gaagaagtgg
      841 ttggcacctt taaccgctgg aaaaagctga acaatgatat tgaagaggct caattattac
      901 tcgatgatcc tgatatgaag gatatggcag cggaagaaat tgcggaaaat aaagccgaaa
      961 tcgagaatct tgagcaacat ttacaaattt tattattgcc gaaagatccg aatgatgaat
     1021 acaacgcctt ccttgaaatc cgtgccggaa cgggcggtga cgaggctggt atttttgccg
     1081 gtgatttata ccgtatgtac agccgttatt gcgaaagcaa acgctggcgg attgaagaaa
     1141 tgtcggcaaa tgagagcgaa caaggcggat ataaagagat catcgtgaaa atttcaggcg
     1201 aaggcgttta cggtcagctt aaatttgaat cgggcggaca ccgtgtacaa cgtgtgccga
     1261 aaaccgaatc acaagggcgt attcacacct ctgcttgcac ggtagcggta atgccggagt
     1321 tgccggaaag cgaaatgccg gaaatcaatc catctgatct gcgaattgac acctaccgct
     1381 catcgggtgc aggcggtcag cacgttaata ccaccgactc tgcggtgcgg attacccata
     1441 tcccgaccgg gattgtggtc gagtgccaag acgagcgttc acaacacaaa aataaagcta
     1501 aagcgttggc ggtgttagcc tctcgtattg tacaagtcga gcaggaacgc caagccgcgg
     1561 agcaagcgga tacccgccgt aacttactcg gttcaggcga ccgttcggat aaaatccgca
     1621 cctacaacta cccgcaaggg cgtgtgaccg accaccgtat taacctcacg gtttatcgtt
     1681 tagacgaagt gatgaacggc aaaattgacg agctgatcca accgattatc actgaatatc
     1741 aagcggatca attggcggcg ttgtctgatc aaaattaagt cacaagcggt taggttttgc
     1801 caattttttg taaaattaga ctgcttgttt atatcttaga ggaatcctga taatactgaa
     1861 atatcatgca aactgataac gttactttat ctgaatggca atttggggag aaaccaaata
     1921 ccctagccaa ccttgtgctt gctggcataa agacggctac ttgttctgga tattgctttt
     1981 ataagttaga caatgagcct cttccgcaaa aaggtgagcg acacatagtg ttagacagcc
     2041 aaaattgtcc tgttgtgatg attgaattaa cttgtataga gcgaaaaaag tggttcgaaa
     2101 ttgatgaaaa gtttgcactt ttagaggggg cggattcctt cagtgagtgg gaaaggacgc
     2161 accgaaattt ttttaacgca cagcttgaac gggcgattga tgagcagttt gaactcgttt
     2221 gtgaacagtt tcatacggta aacatttttt caaaaacgta tgcccaatgg tttcggtttg
     2281 ctgagcagac tctccttgaa aatgccgagc aagatccgtt tttaaacgcc aaatttgatg
     2341 ctaatttgtt gctgcaagca gtcacaaaac gctcaaaatc ggccattttt gctttttccg
     2401 aaacagtgct ttctgaacgt gagctggtgg aattagcgga aaaattagct cgacgtgcca
     2461 aaggtgagcc gatggcatat attttaggct atcgggaatt ttggtcgttg ccgttaaaag
     2521 tttcaccggc aacactcatt cctcgccctg atacggaacg attggttgag ttggcgttgg
     2581 aatatgctaa caagcggtta gaaaatcaga aaatgttgca aattctggat ctcggtactg
     2641 gcacaggggc gattgcgttg gcactggcga gtgaattagg tgagaaagcg caaattatcg
     2701 gcgtggattt ccaaaatgag gcggtgcaac tggcagaaga aaatcgccaa cacttgggct
     2761 taaatcaggt tacattttta caaagcgatt ggttcagttc actggcaaat caacaatttg
     2821 atttaattgt ctcaaatccg ccctacattg atgaagcaga cgaaaatttg acggtgggag
     2881 acgtacgttt tgaaccttta accgccttag ttgccgataa taagggattg agcgatttgc
     2941 aaaaaattat tcaaaatgca ccgctttact taaagccaca aggggcgtta atgctcgaac
     3001 acagctggca acaggggaat gcggttcgtg atttatttga tttaaacttg tgggaacagg
     3061 tggaaactgc tcaagattat gccggcaatg accgagtaac caaagcgatt ttaaaataag
     3121 gcagaaaatg gacgatatta ttgaagaatt gttaaaagag ttgaaagatc cgaaagtaac
     3181 catcagcgaa accgagctga aaaagtttct ttatcgtgaa attttacgat taactacgct
     3241 cattgatagg aatgtaagtg agcaacaagt gtttggtcag atgtctgcgt tggtgaaaaa
     3301 agctcgctat aacgtgaatg gcgaaggcga tgaagagaga attaaccaat tacttgattt
     3361 agtctatcgt gaatggggct ttcattgcta ctatgaagac tatttccaca ccgaaaattt
     3421 attgctaaat caagtgattc gcaaacggcg tggaatgccg gtttcacttg gggcggtggt
     3481 gctttattta gctgcggtat tagatttacc gatttacccg gttaattttc cgactcaact
     3541 tgtgctacgg gctgaaatgc gccagccgaa cggagccact aaagtgcgtt ttatcaaccc
     3601 ctggaacggt gaatttctca ctatcgaaat gctgaataaa tggctggagg gtgaaatggg
     3661 ctttggggtc gaagtcaccc ctgatttatt aagacgggca gaaccacaag agttgctgga
     3721 gagggtcgaa actgtgttta aaatggcact tacccgtgaa ggcaaatatg aagaaacctt
     3781 acgcttgatt gaataccgct tggcatttag tccggaagat ccgtacgaaa tcagagaccg
     3841 aggaatggta ctggcaagta tggattgcta ccaagcagca ttagaagata ttaattattt
     3901 cattgaccaa tgccctgaag atccttctgc cgaaatttta aaaatggaag tgaaaggact
     3961 acaacaaaaa agccaagaaa gtttggtgca ttaagctgag aagctgcccc aaaggggctg
     4021 agctatgatt aacctagtac ggctttgccg tgctaggcag taaaataaga aacaaggact
     4081 ccctatgaca gataaaattg taaaagttgg caatattgaa gtggcgaatc acaagccgtt
     4141 tacccttttt ggtggcatga acgtgctgga aagccgagat atggcactga gagtgtgtga
     4201 gcaatatgtt gaagtcacgc aaaaactaaa cgttccttat gtatttaaag cctcctttga
     4261 caaagcaaac cgttcttcaa tccactctta ccgtgggccg ggaatggaag aaggcttaaa
     4321 gattttccaa gaattaaaac aaacctttgg cgtgaacatc attaccgatg tacacgaaat
     4381 ttaccaatgt aaacctgtgg cagaagtggt tgatgtgatt caacttcctg cttttttggc
     4441 ccgacaaacc gatttagtgg aagcaatggc ccgtaccggt gcggttatta atgtcaaaaa
     4501 accacaattt ttaagccctg ggcaaatggg gaacatcgta gaaaagattg ctgaatgtgg
     4561 caatgataaa gtgattttgt gcgaccgtgg cacaaacttt ggttacgata atctagttgt
     4621 ggatatgctt ggtttcggca ttatgaaaaa agtgtcgaaa ggttgcccgg tgatttttga
     4681 tgtgactcac tcattacaat gccgtgatcc attcggtgcg gcttccggtg gtcgcagaga
     4741 tcaagtaacc gaactggcac gctccggtat ggcgattggt gtagccggct tgttcttaga
     4801 agctcacccg gatccaaact cggcgaaatg tgatggtcca tctgcattac cgctgtcaaa
     4861 attagaagcc tttgtttcac agatgaaagc gattgatgag ttggttaaga gctttgaaga
     4921 aattgatact tccaaataaa ctttctccgc attaaatcct ttcctacttt ctttgcttcg
     4981 ccaaagaaag taggcaaaga aaggcgaccc tacttcgcta cttttcttcg cttagtgtga
     5041 atgggtttaa cgaaaaattt acattcgctt cgctcagagt aaattttcct acccattcaa
     5101 acacgctcag gtagctcaga agggatgtaa gaaatgtaaa aagacagaat aggagaaaat
     5161 aatgttaaat attgtttttc ttgaccgtac cggtattcct gctactcacg agattcctcg
     5221 cccgaatttt ccgcatactt ggacagaata tgctaccacc tctgccgacc aaacttttga
     5281 gcgggtaaaa gaggcggata ttatcatcac cagcaaagtg ctgctccaac gtgatttact
     5341 ggctaaatta cccaagctga aattaattgc gatcacagca accggcacga acaatgtgga
     5401 cttagcagca gcaaaagagt tagggattgc agttaaaaat gtgaccggct attccaccgt
     5461 caccgtgccg gaacacgtca ttggtatgat ttattccctg aaacaccgct tagccgatta
     5521 ccatcgtgat ttaattacat cagatcgctg ggcaacctgt gggcaatttt gctatgtaga
     5581 ttacccgatt cacgatgttc gtggctcaac cttaggcatt ttcggtaaag ggaacatcgg
     5641 ggctgaagtg gcacggctgg cacaagcggt cggaatgcag gttatttttg cagaacatca
     5701 aggcgcaact gaaattcgag aaggctacac cgcctttgaa gaggtattca aacaagcaga
     5761 tattatttca ctacactgcc ctttaaccga gcagactcaa aacctgatta atgcagaaac
     5821 cttagcctta atgaagccga ccgcttatat tatcaatacg ggtcgaggcc cactgattga
     5881 tgaacaggca ttgcttgagg cattagagca aggcaagatt gccggcgctg cgttagatgt
     5941 tttagtgaaa gagccgcctg aaaaagacaa ccctttaatg ttagcagcaa aacgtttacc
     6001 aaatttactg attacaccac acgttgcgtg ggcaagcgac tctgcggtca caacgctagt
     6061 gaacaaagta acacaaaata tggaagagtt tgtagaaaca ggtaaataat ttgtagatac
     6121 cataagatat tgtttagatt tcaatttacc gtttcgaaca accctctccc cttatgggag
     6181 agggacgatt tttcttcatt cagaagaaaa atcagggaga gggaacaagc gggtattttt
     6241 catcagaatt ttgcaaat
//
LOCUS       DANKBV010000131         6232 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.131, whole genome shotgun sequence.
ACCESSION   DANKBV010000131 DANKBV010000000
VERSION     DANKBV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.131"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..398
                     /locus_tag="PWY43_002050"
     CDS             <1..398
                     /locus_tag="PWY43_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019518296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2136903.1"
                     /translation="PEMVERNSGHIINIGSIAGTYPYPGGNVYGGTKAFVKQFSLNLR
                     ADLAGTKVRVSNVEPGLCGGTEFSNVRFKGDDEKAAKVYENVQFVRPEDVANIVLWLN
                     QQPEHVNINRVEVMPVAQTSSALNVARNI"
     gene            410..1600
                     /gene="trpB"
                     /locus_tag="PWY43_002051"
     CDS             410..1600
                     /gene="trpB"
                     /locus_tag="PWY43_002051"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDL2136904.1"
                     /translation="MSDTLLNPYFGEFGGMYVPEILVPVLKELEQAFVDAQQDPTFQA
                     EFADLLKNYAGRPTALTLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRM
                     GKTRIIAETGAGQHGVATALACAMLGMPCVVYMGAKDVERQSPNVFRMRLMGAEVIPV
                     QKGSCSLKDACCEAMRDWAANYDTTHYLIGTAAGPHPFPTMVREFQKMIGEETKRQIL
                     AKEDRLPDAVIAAVGGGSNAIGMFADFIEEQSVKLIGVEPAGKGIETGEHGAPLKHGT
                     TGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNATGRAEYVSITDAEAL
                     EAFQALAKNEGIIPALESSHALAYALKMIKQNPEKEQLLVVNLSGRGDKDIFTVDKIL
                     NGGE"
     gene            1602..2408
                     /gene="trpA"
                     /locus_tag="PWY43_002052"
     CDS             1602..2408
                     /gene="trpA"
                     /locus_tag="PWY43_002052"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDL2136905.1"
                     /translation="MSRFDTLFTALKAKNEGAFVPFVTLCDPGFERSFEIIDTMIKNG
                     ADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDSCFELIAKIRAKYPDIPIGLLLC
                     ANLIFVPIAEVFFQRCQQSGVDAVLIADLPVFAAQEFVPTAEKYGIQPVFICPPNADQ
                     NTIERIAQLTQGYTYLVSRAGVTSAENQAHASNLDNLVESLKQTGAAPILQGFGIAKP
                     EQVKEALRLGADGAISGSAIVKIIERNLSNQANLLKELAEFVQVMKAATK"
     gene            2458..3864
                     /locus_tag="PWY43_002053"
     CDS             2458..3864
                     /locus_tag="PWY43_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDL2136906.1"
                     /translation="MKLTKIALSVVAVISAVAVGGSWYTGKQVEEKYQELVVQANNQL
                     KQLSGEFGANIELKDVQIDRHFFSSNAKYRLEVDLGNGKKLDFVGNDKLHHGPLPLNR
                     LAKLNLAPVMMSAESKLQAPEALKKLMDEHIGTGFSNISYSGDVEGHFDISPIKHSDE
                     TGHIETTPIKVQYSYDQKLKGVDSTVELESLKIKAEEFDFQIQGIVYDVQTENNESYP
                     NLTLGKGSGKINAIEFKSKDGKLSQIKDVVVKGDNLLKGDRLISTGDLEAATFNIEGV
                     EMGKLKMDMAYDLDAKLTNDITPLLSNPQTLENEKTGELLLQLLAKSFKFHINNFSLE
                     NSKGKVDLALLLNMAQFDPQNLGNMQAVLQALSTSKFTSNINRQYAEDIIRQVSIVTE
                     KLGEEEAKAFAKQQVDAVFLNAGVEQYGLRKVDDNNVKIELTIDNGKVNLNGRELPED
                     ELQMALFMIVMGAGSLGQ"
     gene            3987..>6232
                     /gene="polA"
                     /locus_tag="PWY43_002054"
     CDS             3987..>6232
                     /gene="polA"
                     /locus_tag="PWY43_002054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2136907.1"
                     /translation="MATIAKNPLVLVDGSSYLYRAFHAFPPLTNRLGEPTGAMYGVLN
                     MLKSLISQVNPSHIAVVFDTKGKTFRYELFKQYKSHRPPMPDDLRPQIEPLHRIIKAL
                     GIPLISIEGVEADDVIGTLAVQAAKDGKDVLISTGDKDMAQLVNDHIMLINTMNNTLL
                     DREGVIEKYGIPPELIIDFLALMGDSADNIPGVKGVGEKTALALLQGIGSLEQIYANL
                     DQIATLSFRGAKNFAPKLEAEKANADLSYLLATIKTDVELDVTHDQLLTQPQDRAELA
                     QLFEHYEFKRWLNEVNQNANPVTQTSAEKVPNNYQVTQAVRNEENVANVVEIDRSKYE
                     TVDTEQKLSAWIEKLTAARLIAVDTETDSLDAMSANLVGISFALENGEACYIPLGHKQ
                     KAQPQQADMFGDEERSESPSSDELVKNQLNLTACLSKLKPILLNPEIKKIGQNIKYDL
                     TIFANYGIGLQGVAFDTMIESYTQNSTGRHNMDDLAARYLGHKTIPFEELAGKGKNQL
                     TFDKIEIAKAAEYAAEDADVTMKLHQVLWEKLQQEPELVKLFDEIEMPLVGVLSRIER
                     NGVLIDPKKLLTQSAEIEKRLEEVETLVHAEAGQTFNLASTKQLQEILFEKLGLPVLK
                     KTPKGAPSTNEEVLDELAQQGHLVPKLLMEHRGLSKLKSTYTDKLPQLINKKTGRVHT
                     SYNQAVTATGRLSSSDPNLQNIPIRNEEGRRIRQAFVAREGYKIVAADYSQIELRIMA
                     HLSNDEGM"
BASE COUNT         1943 a         1172 c         1425 g         1692 t
ORIGIN      
        1 tgcctgaaat ggtggagcgg aacagtgggc atattattaa tatcggctcg attgccggca
       61 cttaccctta cccgggtggt aatgtatatg gtggtactaa agccttcgtg aaacagttta
      121 gcctaaattt gcgggcagat ttagccggca ctaaagtgcg tgtttccaat gttgagccgg
      181 ggctatgtgg cggtacggaa ttttcaaatg tacgctttaa aggcgatgat gaaaaagcgg
      241 ctaaagttta tgaaaacgtg cagtttgtgc gcccggaaga tgtggcaaat attgtgcttt
      301 ggctgaatca gcaacctgaa cacgttaata ttaatcgggt tgaagtgatg ccggtggctc
      361 aaacttcttc tgccttaaat gtagcgagaa atatttaaaa ggaaatatta tgtcagacac
      421 acttcttaat ccttattttg gtgaatttgg tggtatgtat gtgccggaaa ttttagtgcc
      481 ggtgctaaaa gaattagaac aagcctttgt agatgctcag caagacccaa cattccaagc
      541 tgaatttgcc gatttactca aaaactatgc cggtcgcccg actgctttaa ccctttgtcg
      601 taatttaacc aaaggcagta aaaccaaact ctatttaaaa cgtgaagatc ttttacacgg
      661 tggggcacat aaaaccaacc aagtgctagg gcaaatctta ctcgctaaac gaatgggtaa
      721 aacccgcatt attgccgaaa caggagcagg acagcacggt gtggcaaccg cattagcgtg
      781 tgcgatgtta ggaatgcctt gcgtggtgta tatgggcgca aaagatgtag aacgccaatc
      841 gccaaatgta ttccgtatgc gtttaatggg agcggaggtg attccggtac aaaaaggctc
      901 ttgctcgctc aaagatgcct gttgcgaggc aatgcgggat tgggcggcaa attatgatac
      961 tacccattac ctaatcggca ctgcagccgg cccgcacccg ttcccgacaa tggtgcgtga
     1021 atttcagaaa atgattggcg aagagaccaa acgtcaaatt ttagcaaaag aagaccgctt
     1081 gcctgatgcg gtgattgctg ccgtaggcgg tggatcaaat gctatcggta tgtttgcaga
     1141 ttttatcgaa gagcaatccg tcaaattgat cggtgttgag ccggcaggaa aaggaattga
     1201 aaccggtgaa cacggtgcac cgctcaaaca cggcacaacg gggatctatt tcggtatgaa
     1261 atcgccgatt atgcaggata aagacgggca aattgaagag tcttactcca tttcagccgg
     1321 cttagatttc ccttctgtcg ggccgcaaca cgcttattta aatgcgacag ggcgagctga
     1381 atatgtatcg attaccgatg cagaggcttt agaggcattc caagcattag ccaaaaatga
     1441 ggggattatt ccggcgttag aatcttccca cgccttagcc tatgcgttaa aaatgattaa
     1501 gcagaaccct gaaaaagagc agttattagt ggtgaattta tcgggtcgag gcgataaaga
     1561 cattttcacc gtggataaaa ttttaaatgg aggagagtaa aatgagccgc tttgatacgc
     1621 tatttactgc attaaaagca aaaaacgaag gggcttttgt gccgtttgtg acattatgcg
     1681 atcctggttt tgaacgcagt tttgagatta tcgacacgat gattaaaaac ggggctgatg
     1741 ctttagagct aggctttccg ttttctgacc ccctcttaga cgggccggtg attcaagcag
     1801 cgaataaacg agctttagat ggtggctata gcactgatag ctgttttgag ctgattgcca
     1861 aaattcgagc taaatatcca gacattccta tcggtttact actgtgcgcc aatttaattt
     1921 ttgtgccaat cgcagaagta tttttccaac gttgccaaca aagtggagtt gatgcggtgt
     1981 tgattgctga tttgcctgtt ttcgctgccc aagagtttgt gccaaccgct gaaaaatatg
     2041 gaattcagcc ggtgtttatt tgcccgccga atgctgatca aaacaccatt gaacggattg
     2101 ctcaattaac ccaaggctac acttatttag tttcccgtgc cggtgtaact agtgctgaaa
     2161 accaagctca cgcaagtaat ttagataatt tagtggaaag tttgaaacaa accggagctg
     2221 cacctatttt acaaggtttt ggcatagcca aacctgaaca ggtgaaagag gcgttaaggc
     2281 tcggagcaga cggggcgatt tcaggctcag cgattgtgaa aattattgaa cgtaatttat
     2341 ctaaccaagc taatttgctc aaagaacttg ccgaatttgt acaagtgatg aaagcggcta
     2401 ctaagtaaac tacaagcggt cattttttaa atttttttga aaataaaagg aaacagaatg
     2461 aaattaacaa aaattgctct ttcagttgta gcggtaatta gtgctgttgc agttggcgga
     2521 agttggtaca caggtaagca ggttgaagaa aaatatcaag aattggttgt tcaagctaat
     2581 aaccaactta aacaactgag tggcgaattt ggtgcgaata ttgagcttaa agatgtgcag
     2641 atcgatcgcc atttctttag ctctaatgcg aaatatcgtt tagaagtgga tttgggtaat
     2701 ggtaagaaat tggactttgt tggtaatgat aaacttcatc acggtccgct accgcttaat
     2761 cgtttagcga aacttaattt agctcctgta atgatgagtg cggaaagcaa attacaagct
     2821 cctgaagccc ttaaaaaatt gatggatgaa catattggca ccggtttttc taatatcagc
     2881 tattcgggcg atgtggaagg gcattttgat atttccccga ttaaacatag tgatgaaaca
     2941 ggtcatattg aaaccacgcc aatcaaagtg caatatagct acgaccaaaa attaaaaggg
     3001 gttgatagca ctgttgagct agaaagtctt aagataaaag cagaagaatt tgattttcaa
     3061 attcaaggta tagtttatga tgttcaaaca gaaaataatg aaagttatcc taatttaact
     3121 ttaggcaaag gtagtggaaa aattaacgct attgagttta agtctaaaga tggcaaatta
     3181 tctcaaatta aagatgttgt ggtgaagggc gataatctat taaaaggcga tcgtttaatt
     3241 tctaccggag atttagaggc agccactttc aacattgagg gtgttgaaat gggtaaatta
     3301 aaaatggata tggcatacga tttagatgct aaattaacca atgatattac gcctttatta
     3361 tctaatcctc aaacattaga aaatgaaaaa acaggcgagt tattattaca actattagca
     3421 aaatcattta aattccatat taataatttc tcacttgaaa acagcaaagg caaagttgat
     3481 ttggctttat tactgaatat ggctcaattt gacccacaaa atcttggtaa tatgcaagcg
     3541 gtattgcaag ccctttcaac aagtaaattc acttcaaata tcaaccgtca atatgcggaa
     3601 gatattattc gccaagtctc aatagttaca gaaaaattag gcgaagaaga agcgaaagca
     3661 tttgccaagc aacaagttga tgctgttttt ctcaatgcgg gagttgaaca gtacggatta
     3721 agaaaagtag atgacaacaa tgtgaagatt gaattaacta tagataacgg aaaagtgaat
     3781 ttaaacggac gtgaactgcc cgaagatgaa cttcaaatgg ctctgtttat gattgtgatg
     3841 ggagcaggct cactcgggca ataatcataa tttcccagta aaatgaagcg tatttttgca
     3901 atagcagaaa tacgcttttt ttatcctatt tttcgcctat tcttgtataa taacgccaat
     3961 atttatttga ttataaagag atttttatgg caactatcgc aaaaaatcca ctggtattgg
     4021 tggatggttc ttcctattta tatcgagcat ttcacgcttt ccctccgctc actaatcgct
     4081 taggcgaacc gacaggggca atgtatggcg tgctgaatat gttaaaaagc ctgatttcac
     4141 aagtgaatcc aagccatatt gctgtggtgt ttgataccaa agggaaaacc ttccgttatg
     4201 agttgtttaa gcaatataaa tcgcaccgcc caccaatgcc cgatgattta cgccctcaaa
     4261 ttgagccttt gcatcgcatt atcaaagcct taggcattcc actgatttcg attgaaggtg
     4321 tggaagctga tgatgtgatc ggcacgcttg ctgtgcaagc cgctaaagac ggcaaagatg
     4381 tgttgatcag caccggcgat aaagatatgg cacaattggt gaacgatcac attatgttga
     4441 ttaacacgat gaacaatact ttgttagatc gtgaaggcgt gattgaaaaa tatggcattc
     4501 cgcctgagtt gattatcgac tttctggcat taatgggcga ttccgccgat aacattccgg
     4561 gcgtaaaagg tgtgggggaa aagaccgcat tagctttatt gcaaggcatt ggttcacttg
     4621 aacagattta tgctaatttg gatcaaattg ccaccctttc tttccgtggg gcaaaaaatt
     4681 tcgccccaaa attggaagcg gaaaaagcga atgccgattt atcctatttg cttgctacca
     4741 tcaaaaccga tgtggaactt gatgttactc acgatcaact tttaacacag ccgcaagacc
     4801 gtgctgagct tgctcaatta tttgagcatt atgaatttaa acgctggctg aacgaagtga
     4861 atcaaaatgc caatccggtc actcaaactt cggctgaaaa agtaccgaat aattaccaag
     4921 taacacaagc ggtcagaaat gaggaaaatg ttgcaaatgt ggtcgagatc gatcgtagca
     4981 aatatgagac cgttgatact gaacagaaat tatcagcctg gatcgaaaaa ctcaccgcag
     5041 ctcgtttgat tgcggtggat accgaaaccg acagccttga cgcaatgtcg gctaatttgg
     5101 tcggtatttc ctttgcactg gaaaatggcg aggcgtgcta tattccgctt gggcataagc
     5161 aaaaagccca accgcaacaa gccgatatgt tcggtgatga ggagcgttca gaaagcccgt
     5221 cttccgatga attggtaaaa aatcagctaa atttgaccgc ttgtttaagt aaactcaagc
     5281 cgattttgct taacccggag attaaaaaga tcgggcagaa tattaaatat gatttaacca
     5341 tttttgcgaa ctacggcatt gggctacaag gtgttgcttt tgatacgatg attgaatctt
     5401 acacccaaaa cagcaccggt cgtcataata tggacgattt agccgcccgt tatttggggc
     5461 ataaaacgat tccgtttgaa gagcttgctg gtaaggggaa aaatcaactg accttcgata
     5521 aaatcgagat agcaaaagcg gctgaatatg cggcagaaga tgccgatgtg acgatgaaac
     5581 tccaccaagt attgtgggaa aaactacagc aagagcctga attggtgaag ttattcgatg
     5641 aaattgaaat gccacttgtg ggcgtacttt ctcgaattga acgcaatggc gtgttgattg
     5701 acccgaaaaa attattgacc caatcggctg aaattgaaaa acgtttggag gaagttgaaa
     5761 cattagtaca cgcagaagca gggcaaacct tcaatcttgc ctcaactaaa caattacaag
     5821 agattttgtt tgaaaaatta ggcttgccgg tgttgaagaa aaccccaaaa ggtgcaccct
     5881 caaccaacga agaagtgtta gacgagttgg ctcaacaagg gcatttagtg ccgaagttat
     5941 taatggagca tcgtgggtta agtaagttga aatctaccta caccgataaa ttaccacaac
     6001 tgattaacaa aaaaacaggg cgggtgcata cctcttacaa tcaagcagtc accgcaaccg
     6061 gacgcctttc ttccagcgat ccgaatttgc agaacattcc gatccgcaat gaagagggca
     6121 gacggattcg ccaagctttt gtcgcgagag aaggttataa aattgtcgct gccgactatt
     6181 cacaaattga gctacggatt atggctcatt tatcgaatga tgagggaatg at
//
LOCUS       DANKBV010000132         5891 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.132, whole genome shotgun sequence.
ACCESSION   DANKBV010000132 DANKBV010000000
VERSION     DANKBV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.132"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..973)
                     /locus_tag="PWY43_002055"
     CDS             complement(<1..973)
                     /locus_tag="PWY43_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDL2136908.1"
                     /translation="MSLPNYYQDPSVLHLNTTPHHAYFIPFAKGQTPNQLEREQSELF
                     TLLNGDWKFNFYPSPHDLPEDFLNTKFTHQIPVPSNWQTQGFDAHQYTNINYPIPFDP
                     PYVPHQNPCGVYQREITFNPKPNKRYLLNFEGVDSCLFVYVNGEFVGYGQISHSTNEF
                     DITKQLKQGKNSLVVVVLKWCDGSYLEDQDKFRMSGIFRDVYILEREQNYLQDFFIRY
                     QLNADLSKAELKVETQFSTYTEAVHFQLFDPENRLVCEQTSGDFCQEICNLCLWNAED
                     PTLYTLCLSYQNEIIEQKIGFRQIRVENGVMLFNNAPIKFKGVNRHDS"
     gene            1151..2008
                     /gene="menB"
                     /locus_tag="PWY43_002056"
     CDS             1151..2008
                     /gene="menB"
                     /locus_tag="PWY43_002056"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDL2136909.1"
                     /translation="MLYPSEEFLYAPVEWVDHSEGYTDIRYHKSKDGIAKITINRPEV
                     RNAFRPQTVKEMIEAFSDARFDEKIGVIVLTGEGEYAFCAGGDQKVRGDYGGYKDESG
                     VHHLNVLDFQRDIRTCPKPVVAMVAGYAIGGGHVLHMLCDLTIAADNAKFGQTGPKVG
                     SFDGGWGASYMARLVGQKKAREIWFLCRQYNAQEALDMGLINTVVPYADLEKETVRWC
                     REMLQNSPIALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNEKREP
                     DFSKFRRNP"
     gene            2091..4493
                     /gene="mrcB"
                     /locus_tag="PWY43_002057"
     CDS             2091..4493
                     /gene="mrcB"
                     /locus_tag="PWY43_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HDL2136910.1"
                     /translation="MSDVELNEQSEQEPEQKPKKRNYFLSTLFKIGVIGACSMAFYGI
                     YLDGKIRSKMDGQIWELPAEVYARIESIKIEDNLTLEQTKQALLDNGYRQVSQVATPG
                     DFKIEENQLVLLRRAFPFPETPESQRVLRLTFKQDKLTYIEDLVQGKLIEEFRLDPKL
                     LALLHSDSDEERQALRLQQYPRLLIESLILTEDRRFYQHDGISPIGIARALITNYQAG
                     RTVQGGSTLTQQLVKNLFLSNERSLVRKVNEALMSVILDFRYDKNRILETYLNEIYLG
                     QNGSYQIHGFALASQFYFGRPIQEISLDQIALLVGMVKGPSLYNPWRNPQGAVERRNV
                     VLRLLLDHQVINQELYALLSSRPLNIREKGTIYREQPAFMQALTIDLKNELGEIKSAQ
                     LSGTKIFTTLDRKQQRSAEQAVITGLENLESKNKKVSDLQSAIVVAEYRTGKVRAIVG
                     DRLTQFAGFNRAIQSKRQIGSLVKPSVYAIALAQPELFRLNTPIQNKQISIKVGSTYW
                     TPRNYDRSFSGSVMLMDALVRSLNIPTVNIGMKVGLKKVIAKQKEMGWDNAEIPPYPS
                     TLLGSYSISPYDVTRSYQVIANEGLKTPLTTIEAIISHNGEMFYQRDSGKVSEQVLPP
                     EAAVQTMYAMQQVVERGTARSLQNDFAKLKLAGKTGTTNNARDTWFVGIDGEHVTTVW
                     LGKDNNGDTNLTGSSGALYVYKQYLERALPQAYRLPKLNTMQWVGINSYGSWNCDSGR
                     QIPVWRDKGQRYCTSAGNVVATEKPSVWDALSLKKENIEAKAEEAEGKQPIPTEEVAP
                     IE"
     gene            4668..5174
                     /locus_tag="PWY43_002058"
     CDS             4668..5174
                     /locus_tag="PWY43_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2136911.1"
                     /translation="MAMSNIGLDKEIAKKLADELNGLLATYQVFYTNVRGYHWNVRGV
                     NFFELHAKFEEIYDDLVTKVDEIAERILTLGHTPQNAFSQYLTKSQIQEHIGVSAAQE
                     CLRGTLNGFKTLLGQQREILALASDANDEGTASQMSDYIKEQEKLVWMFTAACAECSD
                     YAQGCSCN"
     gene            5233..>5891
                     /locus_tag="PWY43_002059"
     CDS             5233..>5891
                     /locus_tag="PWY43_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDL2136912.1"
                     /translation="MAMSQGFQFKQFFIRHDRCAMKVNTDGILLGAIAHIKDCQNILD
                     LGTGSGLVALMLAQRTDDCCHITALELEPNAYQQAVENAQNSAWANRISVLQGDVMQQ
                     AFEQKFDLIVSNPPYFADSLAARTTERDLARSISHYSHLNWLAKAKPWLAENGKITLI
                     LPIAAAEKLVDQSEQIGLYCVEQWLIFTKSGKLAKRSIVSFSLNKQKREHKELIIYDL
                     D"
BASE COUNT         1818 a         1117 c         1328 g         1628 t
ORIGIN      
        1 cggaatcgtg gcggtttacc cctttaaatt taatcggggc gttgttgaat agcatcacgc
       61 cattttccac acgaatctga cgaaagccga ttttttgctc aataatttcg ttttggtagc
      121 tcaagcatag cgtgtagagt gtcggatctt ccgcattcca caaacacaaa ttgcaaattt
      181 cttggcaaaa atcaccgctt gtttgctcgc agactaagcg gttttcaggg tcgaaaagtt
      241 gaaaatgcac agcttcggtg taggtggaaa attgtgtttc caccttgagt tctgcctttg
      301 aaagatcagc gtttaactgg tagcggataa agaaatcttg caggtagttt tgctcacgtt
      361 ccaaaatata aacatcacgg aaaatgcctg acattcggaa tttgtcttga tcttccaaat
      421 aactgccgtc acaccatttt agcaccacca cgacgaggct gtttttgccc tgtttaagct
      481 gtttggtgat gtcaaattca ttggtgctgt ggctgatttg cccgtagccg acaaattcgc
      541 cgtttacata aacaaacagg cacgaatcta cgccctcaaa atttagcaaa tagcgtttgt
      601 taggcttggg gttaaacgtg atttcacgct gatacacccc gcaagggttt tggtgtggca
      661 cataaggcgg atcaaacgga atcgggtagt tgatgttagt gtattgatgg gcatcaaagc
      721 cttgagtttg ccagttagac ggcacgggga tttggtgggt aaatttggtg ttgaggaaat
      781 cctccggcag atcgtgcgga cttggataga aattaaactt ccaatcgcca tttagcaaag
      841 taaataactc agattgttcc cgttccaact gattcggcgt ttgcccttta gcaaaaggaa
      901 taaaataagc gtggtgtggc gtggtattaa ggtgcaacac actcgggtct tggtagtaat
      961 tcggtaacga cataaaagcc ctatgatcaa aaagatataa atacgaatac actttaattc
     1021 tcttataaaa tagggcaatc aactatgtga aaaattgtga ggcggatcac aaaataattt
     1081 cctcaccttt ttccttcgat tccgttataa tcacaagaat tttttatgtc atttttcaag
     1141 gaatttacca atgttatatc caagcgaaga atttttatat gcaccggttg agtgggttga
     1201 tcacagcgaa ggttacaccg atattcgtta ccataaatca aaagacggta tcgctaaaat
     1261 caccattaat cgtccggaag tgcgtaatgc tttccgtccg caaacggtaa aagagatgat
     1321 tgaagccttt tctgatgccc gttttgatga aaaaatcggc gtcatcgtat taaccggtga
     1381 aggcgaatat gcgttctgtg ccggcggcga ccaaaaagtg cgtggcgatt acggcggcta
     1441 caaagatgaa agcggtgtgc atcacttaaa tgtgttggat ttccaacgtg atattcgtac
     1501 ctgcccgaaa ccggtggtgg caatggttgc cggctatgcg attggtggtg gacacgtttt
     1561 acatatgtta tgtgatttaa ccattgccgc tgacaatgca aaattcggtc aaaccggccc
     1621 gaaagtgggg tcatttgacg gcggttgggg tgcaagctat atggcacgtt tagtcggtca
     1681 gaaaaaagcg cgtgaaattt ggttcttatg ccgtcaatat aatgcacaag aagcgttaga
     1741 tatgggctta atcaataccg ttgtgcctta tgctgattta gaaaaagaga ctgtgcgttg
     1801 gtgtcgcgaa atgctacaaa acagcccgat tgccttacgt tgcttaaaag cagcattgaa
     1861 tgcagattgt gacggtcagg ctggcctgca agagcttgct ggtaatgcaa caatgttgtt
     1921 ctatatgaca gaagaagggc aagaaggacg caatgcgttc aacgagaaac gtgagccgga
     1981 cttcagcaaa ttccgccgca atccataatt gacggcaaat acaagcggtt cgattttgca
     2041 aaaaatttgc aaaaatcgac cgcttacctt ttctaattta agttaatttt atgtcagatg
     2101 ttgagttaaa cgagcagagc gagcaagagc ctgagcaaaa accgaaaaag cgaaactatt
     2161 tcctttcaac cttattcaaa atcggggtga taggagcttg cagtatggct ttttacggca
     2221 tttatttaga cggtaaaatc cgtagcaaaa tggatggaca gatatgggaa ttaccagctg
     2281 aggtgtatgc tcgtattgaa agcattaaaa ttgaagacaa cttaacgctt gagcaaacca
     2341 aacaagcgtt gctggataac ggctatcgtc aagtttctca agttgcgaca ccaggcgatt
     2401 tcaaaattga agaaaatcag ctggtgttgc tacgccgagc tttcccgttt ccagaaacac
     2461 cggaatcgca acgagtgctt cgtttaactt tcaaacaaga taaattaact tatattgaag
     2521 atttggtgca gggtaagttg atcgaggagt tccgcttgga tccgaaatta ctggcgttac
     2581 tgcattccga tagcgatgaa gaacgtcaag cattacgctt acaacaatac cctcgcttgt
     2641 taattgagtc actgatttta accgaagatc gccgctttta tcagcacgat gggattagcc
     2701 caatcggtat tgcccgtgct ttaatcacta actaccaagc tggcagaaca gtgcagggtg
     2761 gcagtacctt aacgcaacag ttagtgaaaa acttgttctt aagtaatgaa agatcgttgg
     2821 tgcgtaaggt aaatgaagcg ttaatgtcgg tgattttaga cttccgttac gataaaaacc
     2881 gcattttaga aacctatctc aacgaaattt atttagggca gaacggtagc taccaaattc
     2941 acggttttgc attggcaagc caattctatt ttggtcgtcc gatccaagag attagcttag
     3001 accaaattgc gttattggtg ggaatggtaa aaggtccatc actgtataac ccgtggcgaa
     3061 atccgcaagg tgcggtagag cgacgtaatg tggtgttgcg attattgctg gatcatcaag
     3121 tgatcaatca agaactttac gctctgttaa gctcccgccc gcttaatatt cgtgaaaaag
     3181 gcacgattta ccgtgagcaa cctgccttta tgcaggcatt aaccattgat ttgaaaaatg
     3241 agttaggcga aattaagtca gctcaacttt ccggtactaa aatttttacg acccttgacc
     3301 gtaaacagca acgttcagcc gaacaagcag tgattaccgg tttggaaaat ctggaaagta
     3361 aaaataaaaa agtcagcgat ttgcaatcgg ctattgtcgt ggcggaatac cgcacaggta
     3421 aagtgagggc gatagtagga gaccgcttaa cccaatttgc cggctttaac cgtgcgattc
     3481 agtcaaaacg ccaaatcggt tctttagtca aaccatcggt ttatgcgatt gccttggcac
     3541 agcctgaatt attccgttta aatacaccga tccaaaataa acaaatttca attaaggttg
     3601 gttcaaccta ctggacaccc cgtaactacg accgcagttt tagtggctcg gtgatgttaa
     3661 tggacgcctt agtccgctct ctgaatattc caacggtgaa tattggtatg aaagttggct
     3721 taaaaaaagt cattgccaaa caaaaagaga tgggctggga taatgcggaa attccgcctt
     3781 acccatcaac cttgctaggt tcatattcga tctcgcctta cgatgtaacc cgctcttatc
     3841 aagtaattgc caacgaaggg ctgaaaacac cattaaccac cattgaggcg attatttcgc
     3901 acaatggcga aatgttctat cagcgagata gcggcaaagt gagcgagcag gtgcttccac
     3961 cggaagctgc ggttcaaaca atgtatgcga tgcaacaggt tgtggaacga ggtacggctc
     4021 gtagcttaca aaacgatttt gccaaactga aattagccgg caaaaccggt acaaccaata
     4081 acgctcgaga tacttggttt gtgggaattg atggcgaaca cgtcacgacc gtctggctgg
     4141 gtaaagataa caatggggat accaacctca ccggttcaag tggtgcattg tatgtgtata
     4201 agcaatatct tgagagagcc ttgcctcagg cgtatagatt accgaaactt aatacgatgc
     4261 aatgggtggg gattaatagc tatggtagct ggaattgcga tagtggcaga caaattccag
     4321 tttggcgaga taaagggcaa cgttattgta ccagtgcagg taatgtagtg gctacagaaa
     4381 aaccaagtgt ttgggatgca ctttcgctga aaaaagaaaa tatcgaggct aaagcagaag
     4441 aagctgaagg gaagcaacca atccctactg aagaagttgc accaattgaa taaaaataaa
     4501 tttgatagcc attatcattc aagacgcaca atctgatagg ttatacctat aaaaagtata
     4561 tattataacc atttgctagt tttcattttg gattgtaata tagtaataaa ttaaataagc
     4621 agtatctaat ttaatttttt aagattaaca attaagagga gcatattatg gcaatgtcaa
     4681 atatcggttt agataaagaa atcgcaaaaa aattagcaga tgagttaaat ggtttattag
     4741 ctacttacca agtgttttac actaatgtac gtggctacca ttggaatgta cgtggtgtga
     4801 acttctttga attacatgca aaatttgaag aaatctacga tgatttagtg acaaaagtag
     4861 atgaaattgc agaacgtatt ttaaccttag gtcacacacc tcaaaatgcg ttcagccaat
     4921 atttaaccaa gtcacaaatt caagaacata ttggtgtttc agctgcacaa gaatgtttac
     4981 gtggtacttt aaatggtttc aaaacattac tgggacaaca acgtgaaatt cttgctctag
     5041 caagtgatgc aaacgatgaa ggtactgctt ctcaaatgag tgattacatc aaagaacaag
     5101 aaaaattagt atggatgttc actgcagctt gtgctgaatg ttcagactac gcacaaggtt
     5161 gttcttgtaa ctaagttgca ataatataat taacccccga taatgaaaga ttatcggggg
     5221 ttactttttc agatagctat gtcgcaagga tttcaattta aacagttttt tattcggcac
     5281 gacagatgtg caatgaaagt caatacagat ggtattctat tgggagcgat tgctcacata
     5341 aaagattgtc agaatatttt agatctgggt acaggttctg gtttagtagc acttatgctt
     5401 gctcagagaa cagatgattg ttgtcacatt actgcgttgg aacttgaacc aaatgcctac
     5461 caacaagcgg tcgaaaatgc ccaaaattct gcatgggcaa atcggatttc cgttttgcaa
     5521 ggtgatgtga tgcagcaggc atttgaacag aaatttgact tgattgttag taacccacct
     5581 tattttgcag attcacttgc agctcgaacc acagagagag atttagctcg ctcaattagc
     5641 cattacagcc acttgaattg gctggcaaaa gcaaaaccat ggttagcaga gaacgggaaa
     5701 attacactga ttttacctat agcagctgca gaaaaattag ttgaccaaag tgaacaaatt
     5761 ggattgtatt gtgttgaaca atggttaatt tttaccaaga gcggcaaatt ggctaaacgc
     5821 agtatagtga gttttagttt aaacaagcaa aagcgtgaac ataaagagct gatcatctat
     5881 gatctagata a
//
LOCUS       DANKBV010000133         5887 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.133, whole genome shotgun sequence.
ACCESSION   DANKBV010000133 DANKBV010000000
VERSION     DANKBV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.133"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1474
                     /gene="ettA"
                     /locus_tag="PWY43_002060"
     CDS             <1..1474
                     /gene="ettA"
                     /locus_tag="PWY43_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDL2136913.1"
                     /translation="QPGIKIGYLPQEPKLAPQQTVREAVEEAVAEVKNALTQLDEVYA
                     MYADENADFDKLAAKQAELEAIIQAHDGHNLQNQLERAADALRLPDWDAKIEHLSGGE
                     RRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITHDRYFLDNV
                     AGWILELDRGEGIPWEGNYSSWLEQKEKRLAQEQAAESARQKSIEKELEWVRQNPKGR
                     QAKSKARMARFEELNSGEYQKRNETNELFIPPGPRLGDKVIEVQNLTKSYGDRTLIDD
                     LSFAIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIVLGETVVMASVDQFRDAMD
                     DKKTVWEEVSNGQDILTIGNFEIPSRAYVGRFNFKGVDQQKRVGELSGGERGRLHLAK
                     LLQRGGNVLLLDEPTNDLDVETLRALENALLEFPGCAMVISHDRWFLDRIATHILDYG
                     DEGKVTFYEGNFSDYEEWKKKTFGEAATQPHRVKYKRIAK"
     gene            1562..2338
                     /gene="dnaQ"
                     /locus_tag="PWY43_002061"
     CDS             1562..2338
                     /gene="dnaQ"
                     /locus_tag="PWY43_002061"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDL2136914.1"
                     /translation="MTEEKIIRQVVLDTETTGMNMAGGPPQIGHNIIEIGAVEVINRR
                     LTGRTYHVYIKPPREVDEEAIAVHGITNEFLQDKPVFAEIAEEFLDFIKGAELIIHNA
                     PFDVAFMDQEFSYLPNPPAKTAEMCTVTDSLQMARRMYPGKRNNLDALCDRLGIDNSK
                     RVLHGALLDAEILADVFLMMTGGQISLLGEEETEVVSEVIDIEEEFSAIVLNADEAII
                     LKANDEEEESHLALLKLIEKKSKGNCLWTKALTDTAEISH"
     gene            complement(2402..2851)
                     /gene="rplI"
                     /locus_tag="PWY43_002062"
     CDS             complement(2402..2851)
                     /gene="rplI"
                     /locus_tag="PWY43_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL2136915.1"
                     /translation="MQVILLDKVAHLGSVGDQVTVKSGFARNFLIPQGKAVMATAANI
                     AHFEARRAELEAKAAAALATAQERAAKIAALAAVSVSATAGDDGRLFGSVSAKDIAEA
                     LTAAGVGVAKSEVRLGEGPLRTTGEHQVKIHLHPEVNATVAVNVVAE"
     gene            complement(2867..3094)
                     /gene="rpsR"
                     /locus_tag="PWY43_002063"
     CDS             complement(2867..3094)
                     /gene="rpsR"
                     /locus_tag="PWY43_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL2136916.1"
                     /translation="MARYFRRRKFCRFTAENVVEIDYKDIATLKNYISESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLALLPYTDNHQ"
     gene            complement(3069..3443)
                     /gene="priB"
                     /locus_tag="PWY43_002064"
     CDS             complement(3069..3443)
                     /gene="priB"
                     /locus_tag="PWY43_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDL2136917.1"
                     /translation="MNQRKQASNLPIDNCLILSGTVSSLVKQSLNPLGVPHYSFWLEH
                     RSIQTEVNLERQAWCKIQVVLAGNQFSYLTQQIKLGESVRIKGFIHTHKDFNGLNQLV
                     LHTEQIEFIDQEKPNGTLFPSS"
     gene            complement(3418..3792)
                     /gene="rpsF"
                     /locus_tag="PWY43_002065"
     CDS             complement(3418..3792)
                     /gene="rpsF"
                     /locus_tag="PWY43_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL2136918.1"
                     /translation="MRHYEIVFMVHPDQSEQVPAMIERYTASVKEAGGQVHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQSVIDELETNFRYNDAVLRNLIVHTKAAVTEASPM
                     VKAKESKVAEAVAEVESEEAGE"
     gene            4026..5312
                     /gene="purD"
                     /locus_tag="PWY43_002066"
     CDS             4026..5312
                     /gene="purD"
                     /locus_tag="PWY43_002066"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL2136919.1"
                     /translation="MNILIIGNGGREHALAWKVRQSPLADKVFVAPGNAGTAMENGIE
                     NVAISATDVPALVTFAKQNSVGLTIVGPEAPLVVGVVDAFRAHGLTIFGPTQAAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
                     LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRV
                     GEGDSGLNTGGMGAYSPAPVVTKAIHQRVMDEIIYPTVQGMASENTPYTGFLYAGLMI
                     MPNGQPKVIEFNCRFGDPETQPIMMRLESDLVELCLKACEGKLDSVQSKWCEQAALGI
                     VLAAAGYPGDYRKGDEIIGIPTSTSNQKVFLAGVEQKEGKLLTNGGRVLCATALGNSV
                     FEAQQQALQLAEQIQWQGKFYRRDIGYRAVARKQAK"
     gene            5346..>5887
                     /locus_tag="PWY43_002067"
     CDS             5346..>5887
                     /locus_tag="PWY43_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136920.1"
                     /translation="MSTTLNEINITELDAGPEIKSLSLNDAVNSFLNDSACPICGKNC
                     QYWQRLKRSLNRKENQGKGDKVLVKNPSPISIEKNLINAYKEYRKFEEDLKDKIHSQE
                     SIYDLKLAKELFHSYTNLASHLSLEQDKNHFNDKALLKNYPDSLKSALVLAEWTKEYH
                     GKYDNFINDIKSDNWTSAAI"
BASE COUNT         1777 a         1222 c         1343 g         1545 t
ORIGIN      
        1 acaaccggga attaaaatcg gctatcttcc acaagaaccg aaacttgccc cacagcaaac
       61 cgtgcgtgaa gcggtggaag aagcggttgc ggaagtaaaa aacgccttaa cccagcttga
      121 tgaagtctat gcgatgtatg ctgatgagaa tgccgatttc gacaaattag cggcaaaaca
      181 ggcggaatta gaggcgatta ttcaagccca cgatggccat aaccttcaaa accagttaga
      241 gcgtgctgcc gatgcgttac gtctgccgga ttgggacgca aaaatcgagc atttatcagg
      301 gggtgaacgc cgccgtgtgg cactttgccg tctgttgctg gaaaaaccgg atatgttatt
      361 gttagatgag ccgaccaacc acttagatgc ggaatcggtg gcgtggttgg agcgtttcct
      421 ccacgactac gaaggcaccg tagtggcaat tacccacgac cgttacttct tagacaacgt
      481 tgcgggctgg atcttagagc ttgaccgtgg tgaaggcatt ccttgggaag gtaactactc
      541 ttcttggttg gagcagaaag aaaaacgctt ggcacaagag caagcggcgg aatcggctcg
      601 tcaaaaatcg attgagaaag agttggaatg ggtacgccaa aatccaaaag gtcgccaagc
      661 gaaaagtaag gctcgtatgg cacgctttga agagcttaac tcaggcgaat atcaaaaacg
      721 taacgagact aacgaactct ttattccacc gggtccacgc ttaggtgata aagtgatcga
      781 ggtacaaaac ctgactaaat cttacggcga tcgcactttg attgacgact tgtcgttcgc
      841 tattccgaag ggggcgattg tcgggattat cggggcaaat ggtgcgggta aatcaacctt
      901 gttccgtatg ctttctggta aagaacagcc ggattctggt tcaatcgtgc ttggtgaaac
      961 ggtggtaatg gcatcggtcg atcagttccg tgatgcgatg gacgataaga aaaccgtgtg
     1021 ggaagaggtg tcgaacgggc aggatattct cacgattggt aactttgaaa tcccaagccg
     1081 tgcttatgtg gggcgtttca acttcaaagg cgtagatcag caaaaacgtg tgggcgagct
     1141 ttcaggcggt gaacgtggtc gtttacactt ggcgaagcta ctacaacgtg gcggtaacgt
     1201 gttgttactg gacgagccga ccaacgactt agacgtagaa accctgcgtg cgttggaaaa
     1261 tgcgctctta gagttcccgg gctgtgcaat ggtaatttcg cacgaccgtt ggttcttaga
     1321 ccgtatcgcc acccatattt tagactacgg cgatgaaggc aaagtgacgt tctacgaggg
     1381 taacttctcc gactatgaag agtggaagaa gaaaaccttc ggtgaagccg ccacccaacc
     1441 gcaccgagtg aaatataaac ggattgcgaa gtaacaatgt aggggcgaaa tatgtttcgc
     1501 ccctaatcaa atgcagaatt aagggataaa aatatttcac ccctacaatt ttaaattgaa
     1561 tatgacagaa gaaaaaatta tccgccaagt agtgctggat actgaaacca caggaatgaa
     1621 tatggcaggt ggcccgccac agattgggca taatattatt gagatcggtg cggttgaagt
     1681 cattaaccgc cgtttaaccg gcagaactta tcacgtttat atcaagccac cgcgtgaggt
     1741 agatgaagag gcgattgcag ttcacggcat caccaatgaa tttttgcaag ataaacctgt
     1801 ttttgctgaa attgccgagg agtttttgga tttcatcaaa ggtgcggaat taattattca
     1861 taatgccccc ttcgatgtgg cgtttatgga tcaagaattt tcttatctgc ctaatccacc
     1921 ggcaaaaacc gcagaaatgt gtaccgtaac cgatagcctg caaatggcac gaagaatgta
     1981 ccccggcaaa cggaataatt tagatgcact ttgcgacagg ctcggcattg ataatagcaa
     2041 gcgtgtacta cacggggcgt tactggatgc ggagatctta gctgatgtat tcctaatgat
     2101 gaccggcggt cagatttcat tattaggcga agaagaaact gaagtggtaa gtgaagttat
     2161 tgatattgaa gaagaatttt ctgcaattgt gttaaatgcc gatgaggcaa ttattcttaa
     2221 agcaaacgat gaagaggaag agtcgcattt agcacttctg aaactgattg agaaaaaatc
     2281 caaaggaaat tgcctatgga caaaagcatt aaccgacaca gcagaaatat ctcactgatt
     2341 ctaaccaata aataaagcaa acaaaaaacc cagctttcgc tgggttttct atttctactg
     2401 attattcagc aacaacgttt actgctacag ttgcattcac ttcagggtgt aagtgaattt
     2461 taacttggtg ttcgccggtt gtacgaagtg gaccttcacc taaacgaact tcagatttag
     2521 caactccaac acctgctgca gttaatgctt cagcgatgtc tttagctgat acagaaccga
     2581 ataaacgacc atcatcacct gcagttgcag atactgatac tgctgctaat gctgcaattt
     2641 tcgctgcacg ttcttgtgca gttgctaatg ctgctgcagc ttttgcttct aattctgcac
     2701 gacgtgcttc aaagtgagca atgtttgccg cagttgccat aaccgcttta ccttgtggga
     2761 ttaagaagtt acgagcaaaa ccagatttaa cggttacttg gtcgcccaca gaacctaagt
     2821 gagcaacttt gtctaataaa ataacttgca ttgtactctc tcctgattat tggtggttat
     2881 cagtatatgg aagtaacgca aggtagcgag cgcgtttaat tgcacgagct aattgacgtt
     2941 gatacttcgc acgagtacca gtaatacggc taggaacaat cttgccgctt tcagaaatgt
     3001 agttctttaa tgtagcgata tctttgtaat cgatttcaac aacattttcc gctgtgaaac
     3061 ggcagaactt acgacgacgg aaataacgtg ccatttggct tctcctgatc tataaattca
     3121 atttgttcgg tgtgtaagac taactgattt aaaccgttaa agtctttgtg agtatggata
     3181 aaacctttaa ttctcacgct ctcaccgagc ttaatttgtt gggttaaata actaaattga
     3241 ttgcctgcca aaaccacttg gattttgcac cacgcttggc gttctaaatt cacttcggtt
     3301 tggattgaac gatgttctaa ccaaaaactg taatgaggca caccaagggg gtttaggctt
     3361 tgtttaacca agctggaaac cgttccgctt aagattaaac aattatcaat cggcaaatta
     3421 ctcgcctgct tcctctgatt caacttcagc taccgcttca gcaactttgc tttctttcgc
     3481 tttcaccatt ggtgacgctt cagttactgc cgctttagtg tgaacgatta agttacgaag
     3541 aactgcatcg ttataacgga agttagtttc taactcgtcg attacacttt gaggtgcttc
     3601 tacattcatt aacacatagt gtgctttgtg aagtttgttg attgggtatg ctaattgacg
     3661 acgaccccaa tcttctaagc gatgaacttg accgccagct tcttttacag acgctgtgta
     3721 gcgctcaatc attgctggta cttgttcgct ttggtccggg tgaaccataa aaacgatttc
     3781 gtagtgacgc attgactatc cttacgggtt agcagcctcc aactttagcc agtcaccaac
     3841 ttgtggaagc aaggattaaa aaacgaatgt gactgaacgg caggattata ctgaaaaaag
     3901 cctattttgc aagcaacaat ttcgtatttt gcaaaaaatc cgagaaaaac gaccgcttgt
     3961 cagcagataa acaaatcaag tacaatacgc aaacgtttgc tttatcattt ttgcaaggaa
     4021 tcccaatgaa tattttaatt atcggcaacg gtgggcgtga acacgctctt gcttggaaag
     4081 ttcgtcaatc gccgctcgca gacaaagtct ttgtcgcccc cggcaatgcc ggcacagcga
     4141 tggaaaacgg cattgaaaat gtagctattt ccgccactga cgtgcctgcc ttagttacat
     4201 tcgctaaaca aaatagcgta ggtttgacga ttgtcggccc tgaagcaccg ttagtagttg
     4261 gcgtggttga tgcttttcga gcccacggtt taaccatttt cggcccgacc caagccgctg
     4321 ctcaattaga aggttctaaa gcatttacca aagatttctt ggctcgccat cagattccaa
     4381 ccgcagaata ccaaaacttc accgaaattg aaccggcact tgcctactta aaacaaaaag
     4441 gtgcaccgat tgtaatcaaa gcagacggtt tagccgcagg caaaggcgta attgttgcaa
     4501 tgactttaca agaggcggaa gatgcagtgc gtgatatgct gtcaggcaac gcctttggtg
     4561 aagccggcag ccgtgtggtg atcgaggaat ttttagacgg agaagaggcc agttttattg
     4621 tgatggtgga cggcaaaaat gtcgaaccaa tggcaacctc acaagaccac aaacgtgttg
     4681 gcgagggcga tagcggctta aataccggcg gaatgggggc ttactcccct gctccggtgg
     4741 taacaaaggc aatccaccaa cgtgtaatgg acgaaattat ttacccgacc gtgcaaggaa
     4801 tggcaagcga aaatacccct tacaccggtt ttctctatgc cggcttaatg attatgccaa
     4861 acggtcagcc gaaagtgatt gaatttaact gccgttttgg agaccctgaa actcagccga
     4921 ttatgatgcg tttggaatca gacctcgttg aattgtgctt aaaagcgtgc gaaggcaaat
     4981 tggatagcgt gcaatcaaaa tggtgtgaac aagccgcatt aggcattgtt ttagctgccg
     5041 caggctaccc gggcgattac cgtaaaggcg atgaaatcat aggcattccg acctctacct
     5101 ccaatcaaaa agttttctta gcaggggttg agcaaaaaga aggaaaactg ctcaccaacg
     5161 gtggacgtgt actttgtgcc accgcacttg gcaacagtgt atttgaagct caacaacaag
     5221 ccttacaact tgccgaacaa attcaatggc aaggcaaatt ctatcgccga gatattggct
     5281 accgagcagt ggcaagaaaa caagcgaaat aatttataaa ctcttaaaac aaggaaaaat
     5341 ttattatgag tacaacactt aatgaaatta atatcactga actcgatgca ggccccgaaa
     5401 ttaaatcact atcattaaac gatgcagtaa actcttttct aaatgactct gcgtgcccta
     5461 tatgtggaaa aaactgtcaa tattggcaaa gactaaaaag atctttaaat agaaaagaaa
     5521 atcaaggtaa gggagataaa gttcttgtga agaatccatc tccaatttct attgagaaaa
     5581 atttaattaa cgcttataaa gaatacagaa aatttgaaga ggatttaaag gacaaaatcc
     5641 actctcaaga aagtatatat gatctaaaac tagctaaaga attatttcat tcttatacaa
     5701 atttagctag tcatttaagt ttagagcaag ataaaaatca ttttaatgat aaagcattat
     5761 taaagaatta tcctgatagt ttaaaaagtg ctttagtact tgccgaatgg actaaagaat
     5821 atcatggcaa atatgataac tttattaatg acatcaaatc agataattgg acaagtgcag
     5881 caattaa
//
LOCUS       DANKBV010000134         5820 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.134, whole genome shotgun sequence.
ACCESSION   DANKBV010000134 DANKBV010000000
VERSION     DANKBV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.134"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(149..445)
                     /locus_tag="PWY43_002068"
     CDS             complement(149..445)
                     /locus_tag="PWY43_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136921.1"
                     /translation="MSNTIQTSNHTLSSTAECLSTAEMSLLVRLLKLARTQHELILTA
                     LPALQQLQSPLAEQAEATTQQAAILRLVEETLLARYPTIQDAELKQQIFEWFNS"
     gene            602..1060
                     /locus_tag="PWY43_002069"
     CDS             602..1060
                     /locus_tag="PWY43_002069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136922.1"
                     /translation="MDKAFNDEYHPLATDLINDIFTVQGISNRGRIALIRQYAELLVR
                     SLLGVSFSERITLGDKKTTRALAELKAKRQHYYELAVAVEYIRDVGNAATHTLYTGEI
                     SGKMVNKSLFTWHKFMPIISLSTFTNIRLARMLPPWCCLGCCRLVFACWC"
     gene            1108..1443
                     /locus_tag="PWY43_002070"
     CDS             1108..1443
                     /locus_tag="PWY43_002070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136923.1"
                     /translation="MIILLFKVHGIERMTLWVERNRRKLEQIDRLAVENEEQVTQFIE
                     KEYSAYDFALAQMGANIHPVPYQDFDVAAARYREYVSEMSVLQSPEIVELKGIMDFVY
                     LGVRQEKAR"
     gene            complement(1459..2286)
                     /locus_tag="PWY43_002071"
     CDS             complement(1459..2286)
                     /locus_tag="PWY43_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136924.1"
                     /translation="MPTEITRLTLRFDGLDATEHQVDLYSLGEALQGFARIISTAGHF
                     SVTQTYSKSFSMHSVKTYAQEVRANCFSVDMVLNWAQQNQILSGSFGILLAAVIPYIF
                     QRNKAKTEEMKMLKDSLDKAIEALGNRDQAVIERLASTIEKMAEELRPAARLAVSPIG
                     ESCKTISIIDTQTKQSIGIINESDKESIMSLSENEITGIQVFDILISELDKVKATCKV
                     NINNAEQRTNAVISDPLFGSAENPYLQAFAQNKAIKVTGKATIKDGEIIKLTIMDIH"
     gene            complement(2303..3142)
                     /locus_tag="PWY43_002072"
     CDS             complement(2303..3142)
                     /locus_tag="PWY43_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2136925.1"
                     /translation="MKWYEFAEQQRKICGLTQVELGDKLNVEQGTVGNWLNGRREPEL
                     ATILKIFHALGITQVTFNSDSQIVDIQSKQRIHWYELAEQRRKNLGLSQAELADEFEV
                     SQATVGNWLSNRREADIYTIAKLLERLGIVDVVLHTDATISLTSGIFDTNTPQLKVSA
                     ALKFRGQDIGEKLTLEDELSLHYFSQDVRAYAVRIDGEKLEPRIVSGEYIVVEPGAKL
                     QSYDEVLIKLKDGRYMIRVLLTGRNKEWRYADPNTMQQDTDFDPSQIETMEYIAAIIK
                     PPR"
     gene            complement(3142..3801)
                     /locus_tag="PWY43_002073"
     CDS             complement(3142..3801)
                     /locus_tag="PWY43_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136926.1"
                     /translation="MKEKWNDIVKRRIKELDIHRTVFAERVGVSSSGLGSWLNESRVP
                     NLDQIAKMLYLVGIDSVTLHSADCLMSVRESYKLNKIEGVPVKGSLAFGFPEMGNAVE
                     KATLKTAEQKQFIDFRASSPRSEAYQILGTALSPRIRRNEFVVIDPNKKPLETDEVLV
                     YFKETEEYAIKRLVELAKKEGDPVFLADLNQDMLRMAVEEDAITMYRIEGFAKESKVI
                     R"
     gene            3898..4092
                     /locus_tag="PWY43_002074"
     CDS             3898..4092
                     /locus_tag="PWY43_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2136927.1"
                     /translation="MNKIRQFRRAIGLSQQELASVIGVTTGAIGFYERGLREPNLATI
                     KKLAEALNCKPADLYPVLEG"
     gene            4141..4584
                     /locus_tag="PWY43_002075"
     CDS             4141..4584
                     /locus_tag="PWY43_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136928.1"
                     /translation="MTMKQTIIEMIESCTGGKSAVAGFLGFTESELNNRLYQTKGQRF
                     KDEELIAIEQEFGVSHWSDEINRQLGKVAFCVPNADELDAEELSELQLHELAKRGLLF
                     AELEKALADGEVTQAEAEVLSKLLNRSQCATAKVITATIELYSKR"
     gene            4647..5486
                     /locus_tag="PWY43_002076"
     CDS             4647..5486
                     /locus_tag="PWY43_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL2136929.1"
                     /translation="MNQLLNISEQKSSITMSSREIAELCEKEHRHVLRDIRAYVGAII
                     QMERGINVKSLDWSGKEGVELFGHTPIGGVICRYEVNPQNNQSYPIYYLDKSATLTII
                     SGYNILLRKKIIDRWQELETQVPQSSKTPHIPQTYAEALRLAADQAEQNQLLQLENKQ
                     KTEENNALKSYFEPGLTPAQFVKGLNGVNSNKINDYLRTRGWLYKEKHCWRVCSQVRD
                     KYLTESFKRIEVDPVERIEMTTYKPILLEKGAVKIFEAYLQGKLPMKADWNGKYYHSK
                     VAV"
     gene            5483..>5820
                     /locus_tag="PWY43_002077"
     CDS             5483..>5820
                     /locus_tag="PWY43_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136930.1"
                     /translation="MRFTSTINNVRLVEWEINITQGALVDLINQASSWAKAVVIDGIT
                     YYWMSFSKVCEELPAVFSKEDTVYRQYKVLKEKGIIDHFKMDGKDYVRLTEKGCEWNK
                     FEPIRESEKNP"
BASE COUNT         1661 a         1122 c         1302 g         1735 t
ORIGIN      
        1 cattgagccg tagctcgttt tgatgggaga ataatacatt atgtgtcaaa ttaagatcaa
       61 tacaaaaaga gtgaaaaatt atacaaaatg tgttgattgg ctatttttta atctattaag
      121 ggtaaaaagc cccttccggg gcttgtgatt atgaattgaa ccattcaaag atttgttgct
      181 tgagttcagc gtcttgaatt gtggggtagc gagcaagtag ggtttcttca acaaggcgga
      241 ggattgccgc ttgttgagtg gttgcctcgg cttgttctgc taatgggctt tgcagttgtt
      301 gcaatgccgg tagggctgtt aaaataagct cgtgttgtgt gcgagctaat tttaacaggc
      361 gaaccaagag gctcatttct gcggtggata agcattcggc agttgagcta agtgtgtgat
      421 ttgatgtttg aatagtattt gacatagcgt cctccgttga tctaaaaaca aattgccctg
      481 aacattcagc gacattgtgt gaagtgaggc ttttttagat cggtacgctg tcgatatgat
      541 gttaaaataa tgcaaatgaa ggttattgtc tgtgatcaat ttcatatttt gaggataaaa
      601 aatggataaa gcatttaacg atgaatatca ccctttagcg accgatttga ttaatgatat
      661 tttcacggtg caagggattt caaatcgggg cagaatagcc cttattcgcc agtatgcgga
      721 gctgttggtg cggtctttac tcggcgtgtc attttcagag cgtattacgc tgggcgataa
      781 gaaaaccact cgtgcgttgg cggagttaaa agccaaaagg cagcactatt acgagctggc
      841 ggtagcggtg gaatatattc gagatgtggg gaatgccgca acgcatacac tctacaccgg
      901 tgagatttcg gggaagatgg taaataaatc actattcact tggcacaaat ttatgcctat
      961 tatttcgttg agtactttca caaatatccg cttggctcga atgctgccgc cttggtgttg
     1021 tttgggttgt tgccgcctcg ttttcgcttg ttggtgttga tccgattgca tcgtcaattt
     1081 cctcaaagtt atgccattgc ggaaaagatg attattttat tgtttaaggt gcatggcatt
     1141 gagcgaatga cattatgggt ggaacgaaac cggcgtaagc tggagcaaat agatcggttg
     1201 gcggtagaaa atgaagaaca ggtgacgcag tttatcgaaa aagagtactc cgcttatgat
     1261 tttgcccttg ctcaaatggg ggcgaacatt caccctgtac catatcagga ttttgacgtg
     1321 gcggcagctc gttatcgtga atatgtatcg gaaatgagcg tgctgcaatc gccggagatt
     1381 gtagagctga aagggataat ggattttgtg tatttggggg tgaggcaaga aaaagcccgc
     1441 tgatggcggg cagaaaattt agtgaatatc cataatcgtc agtttgatga tttcgccgtc
     1501 ttttatggtt gctttgccgg ttactttgat tgctttgttt tgagcaaagg cttgtaaata
     1561 aggattctct gcactgccaa atagcggatc tgaaatcacc gcatttgtgc gttgctcagc
     1621 gttattgatg tttactttac aagttgcttt aactttatcc agctctgaaa tcagtatatc
     1681 aaatacctga atcccggtta tttcattttc agacaaggac ataatcgatt ctttgtctga
     1741 ctcattaatt atgcctatag actgcttggt ttgtgtatca ataatggaga ttgttttaca
     1801 actttccccg attggcgaaa cagcaagtct tgctgcaggg cgaagctctt ccgccatttt
     1861 ttcaatggtg gaagccagtc tttcaataac agcttgatct cgattaccca aagcctctat
     1921 agccttgtct aacgagtctt tcaacatttt catttcttct gtttttgcct tgtttcgctg
     1981 gaaaatataa gggataaccg ctgcaagcag tatgccaaat gaaccggata atatctgatt
     2041 ctgttgagcc cagtttaata ccatatcaac cgaaaagcaa ttcgctctga cttcttgtgc
     2101 ataagttttc acgctatgca tagaaaaact tttagagtag gtttgagtaa ctgaaaaatg
     2161 accagcagtt gaaatgatgc gagcaaagcc ttgtaaggct tcgccgaggc tgtaaagatc
     2221 aacctgatgc tctgttgcat ccaatccgtc aaatctaagg gttaatcggg ttatttctgt
     2281 tggcattgtt actccttatt aattatctcg gcggcttaat aatggcggcg atgtattcca
     2341 ttgtctcaat ttggcttggg tcaaaatcgg tatcttgttg cattgtatta ggatcagcat
     2401 atcgccattc tttatttctg ccggtcagta ataccctaat catatatctg ccgtctttga
     2461 gtttaatcaa cacttcatca tagctttgga gcttcgctcc cggctctacc acgatatatt
     2521 cgccactcac aatgcgaggc tcgagttttt cgccatcaat ccgtactgca taggcacgca
     2581 catcttgaga gaagtaatgt agtgacaatt cgtcttctag cgttaatttc tccccgatgt
     2641 cctgccccct aaattttaag gcggcactga ctttgagttg tggtgtattt gtatcaaaaa
     2701 tacctgaggt caaagaaatt gtggcgtcgg tatgtaatac cacatcgaca atccctaatc
     2761 gctccaataa tttagcaata gtgtaaatat ccgcttctct acggttacta agccaattcc
     2821 caaccgttgc ttgagaaacc tcaaattcat ctgccaactc agcttgtgaa agccctaggt
     2881 ttttacgtcg ctgttcagct aattcatacc agtgtattct ttgtttactt tgtatatcga
     2941 ctatttggct atctgaatta aaggtaactt gagttatgcc taatgcgtgg aaaattttta
     3001 atatggttgc tagttcaggc tcacgtcgtc catttaacca atttccaaca gtgccttgct
     3061 caacgtttaa tttatccccc agctctactt gggtaagccc gcatattttc ctctgctgct
     3121 ctgcgaactc ataccatttc attaccgaat caccttactc tctttcgcaa agccttcgat
     3181 tcgatacatt gtgattgcat cttcctcaac agccatacgc aacatatctt gattcaggtc
     3241 ggctaagaaa acggggtcgc cctctttttt cgctagttcg actaaacgtt tgattgcata
     3301 ttcttccgtt tctttgaaat acaccagcac ttcgtctgtt tcgagtggtt ttttatttgg
     3361 atcgatcacc acaaattcat tgcggcgaat acgaggactg agagccgtgc ctaaaatttg
     3421 ataagcctct gaacgagggg aagaggctct aaaatcaata aattgttttt gctcagcggt
     3481 tttgagtgtc gccttttcta cggcattgcc catttcgggg aagccgaaag cgagcgagcc
     3541 ttttaccggc acaccttcaa ttttattgag tttatagctt tctcttacag acattaaaca
     3601 gtctgcacta tgtagtgtta cgctatcaat gcctactaag taaagcattt tggctatttg
     3661 gtcgaggttt ggcactctac tttcatttaa ccaacttcct aaaccagaag atgaaactcc
     3721 cactcgctct gcaaaaacag ttcgatgaat atccagctct ttaattcgac gtttaacaat
     3781 gtcattccat ttctctttca taggtgcatt ttacctcata aaaccagtaa ataagttatc
     3841 tcctctttac aataaataac actaaatgta ttattgcaca ttaagaggtt ttaatttatg
     3901 aataaaatta gacaattccg gcgggctata gggttatctc aacaagaatt agcgtctgta
     3961 attggtgtta ccactggtgc tattggattc tacgaaagag gtttacgaga acccaaccta
     4021 gccacgatta aaaaacttgc tgaagcctta aactgcaagc cagcggattt atacccggtg
     4081 cttgaaggtt agtttaggct tttggctgaa aatgaaaacc acaaaaaacg ataggaaatt
     4141 atgacgatga aacagacgat tatcgagatg atcgagagtt gcaccggtgg caaatcggct
     4201 gtagccggct ttcttggatt tactgagagc gagctaaaca accgcttata ccaaaccaaa
     4261 ggacaacgct ttaaggacga agagctgatt gcgattgagc aggaatttgg cgtaagccat
     4321 tggagtgatg aaattaatcg tcagcttggc aaggtggcgt tttgtgtgcc gaatgcagac
     4381 gagctagatg cggaggaact ttctgaactg cagttacacg agcttgcgaa acgaggcttg
     4441 ttatttgcag agctggaaaa ggcattggcg gacggcgagg ttactcaagc cgaggctgag
     4501 gtattaagta agctattaaa ccgttcgcaa tgtgcaacgg caaaggtgat tacagcaacc
     4561 attgagctgt atagcaaacg ttaggcaata aaaaaccacc gctgtaacgg tggctcttta
     4621 aaactaaaca acgaaaggta ttttcgatga atcaattatt aaacatttca gaacagaaat
     4681 caagcattac gatgagtagt cgtgagatag cagagttatg cgaaaaagaa catcgtcacg
     4741 ttttaagaga cattagggct tatgttggtg ctattatcca aatggagaga ggaattaatg
     4801 ttaaatctct tgattggagc ggaaaagagg gtgtagaact ttttggacat acccctattg
     4861 gcggagttat atgtcgatat gaggttaatc ctcaaaataa tcaaagttac ccaatctatt
     4921 acctagataa atcagcaacg ctgacaatta tctctggcta caatattttg cttagaaaga
     4981 aaattatcga ccgctggcaa gagctggaaa cgcaagtgcc gcaatcttct aaaacacctc
     5041 atattccaca aacgtatgcg gaagcgttac gtttggcggc agaccaagca gaacaaaatc
     5101 agttattgca gttagagaac aagcaaaaga ccgaagagaa caatgcactg aaatcgtact
     5161 ttgaacctgg tttaacacca gcccaatttg tgaaagggct gaatggcgta aacagcaaca
     5221 aaattaatga ttatttgcga actcgtggct ggttatacaa agaaaagcat tgttggcgag
     5281 tgtgtagcca agttcgggat aagtatctaa cggaatcatt taagcgaatt gaggttgatc
     5341 ctgtagagcg tattgagatg actacctaca agccaatttt gcttgagaaa ggtgcggtta
     5401 agatttttga ggcttattta caaggtaagt taccaatgaa agctgattgg aacggcaaat
     5461 attatcacag taaggtggcg gtatgagatt cacatcaaca ataaacaacg tccgcttagt
     5521 ggagtgggaa atcaacatca cgcagggggc attggtcgat ttaatcaatc aagcctccag
     5581 ttgggcaaaa gcggtggtta ttgacggcat aacctattac tggatgtcgt ttagcaaagt
     5641 gtgcgaagag ttacctgctg tatttagcaa agaagatacg gtttaccgcc aatataaggt
     5701 gttgaaagaa aaaggcatta tcgaccactt caaaatggac ggtaaagatt atgtccgttt
     5761 aactgaaaaa ggttgtgaat ggaataaatt cgagccgatc cgagagtcgg aaaaaaatcc
//
LOCUS       DANKBV010000135         5769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.135, whole genome shotgun sequence.
ACCESSION   DANKBV010000135 DANKBV010000000
VERSION     DANKBV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.135"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..925
                     /gene="metG"
                     /locus_tag="PWY43_002078"
     CDS             <1..925
                     /gene="metG"
                     /locus_tag="PWY43_002078"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HDL2136931.1"
                     /translation="NKLVNLASRNASFIAKRFEGKLAEKLDDEALFNEFTAQAETIAN
                     FYENREFNKAIRAIMELTDKANKYIDDKAPWVIAKEEGKEAELQAVCSMGIELFRVLM
                     SYLKPVLPKLAERAEAFLQSELRWDNIATPLFGHTVAPFKSLFSRLEKKQIDAVIDET
                     KALFAEANKKTENQTKGTQKVEQQAVEKQENIAVEPISPEITIDDFAKLDLRAAKVLN
                     CEAVPKSDKMLRFELDLGDHQRQVFSGIKAAYPNPEALIGRFVIVIANLAPRKMSFGM
                     SEGMILSAGTGGANLFLLDADSGVQPGMQVK"
     gene            935..1351
                     /gene="ndk"
                     /locus_tag="PWY43_002079"
     CDS             935..1351
                     /gene="ndk"
                     /locus_tag="PWY43_002079"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL2136932.1"
                     /translation="MIQQTLSIIKPDATRRNLIGKILAQLEENGLTIKALKKVHLTRE
                     QAEGFYAEHKGKDFFEPLVEFMTSGSIVVSVLEGENAINHYRALMGATNPDNREKGTI
                     RDLYALSMRENSVHGSDSEESAKREIAYFFVPSEIV"
     gene            complement(1408..1884)
                     /gene="greA"
                     /locus_tag="PWY43_002080"
     CDS             complement(1408..1884)
                     /gene="greA"
                     /locus_tag="PWY43_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL2136933.1"
                     /translation="MKQIPMTVRGAEQLREELDFLKNVRRPQIIDAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVTKMANNGKVIFGATVQLVNADTDEE
                     VTYRIVGDDEANIKEGLISVNSPIARGLIGKEVNDSVSITTPGGKVEFDIVGVEYI"
     gene            1981..3426
                     /gene="dacB"
                     /locus_tag="PWY43_002081"
     CDS             1981..3426
                     /gene="dacB"
                     /locus_tag="PWY43_002081"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDL2136934.1"
                     /translation="MIFKNFTRAFLNKFILSTLLIPSITQAEINTEELLNTLPAGTSV
                     SFIAKNLDTNQIITQYQSDIFMLPASTQKVFTALAAKLTLTDDFRFQAALLSNGKVEN
                     GVLKGSLIARFSGDPELTSGQIYQLISKLKQQDINKIEGDLILDTSVFASHDKASGWI
                     WNDLTMCFNAPPAAINVDHNCFYVTLNADQPIGEFAKVNVPSAYPVQVFSSAYIVEPK
                     EAPFCQLDVVVHDNNRYQIKGCMARQSQPFGLSFSVQDPTNYGANIIKAHLKSLKIAF
                     NGQVKESLTAQNGTLLAEHYSEPLPVLLKKMMKKSDNQIADALFRTVANKQHNRPASF
                     QLGSYVIRRLLKTKANIDFKNSVVTDGSGLSRHNQVSSRTMLESLEYIAQNEESLKLF
                     ETFPIAGVDGTISGRGSISTEPLAKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFI
                     NGYSTGELESKTKRAPLNTFENKFYMALFNE"
     gene            complement(3513..3782)
                     /locus_tag="PWY43_002082"
     CDS             complement(3513..3782)
                     /locus_tag="PWY43_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2136935.1"
                     /translation="MINHNLADFVFEYFTENNLKAGTLRYRYIKENVIDAYSTRVDDA
                     FQGKGIAGELYNALIAFAQEKGLKIKPSCSYIEVKMQRSHRDLIA"
     gene            3954..5543
                     /gene="purH"
                     /locus_tag="PWY43_002083"
     CDS             3954..5543
                     /gene="purH"
                     /locus_tag="PWY43_002083"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL2136936.1"
                     /translation="MAIQQALLSVSDKKGIVEFARGLSARGVKLLSTGGTAKLLAEAG
                     LLVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGTDDEVMNQHGIERIDMVVVNLY
                     PFAQTVANPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQHQ
                     NSLTLETRFNLAIKAFEHTAQYDSMIANYFGQKVPPYQGAEEEDLTQPSGQFPRTLNL
                     NFVRKQTMRYGENSHQNAAFYVENELKEASVSTAKQLQGKALSYNNIADTDAALECVK
                     EFSEPACVIVKHANPCGVALGKNILDAYNRAYQTDPTSAFGGIIAFNRELDGETAQTI
                     ADRQFVEVIIAPTVSASAKEVLATKKNVRVLECGEFSEAVKRLDFKRVNGGLLVQDAD
                     QGSVSIDDLKVVSERKPTEAELKDLLFCWKVAKYVKSNAIVYAKDGQTIGIGAGQMSR
                     VYSAKIAGIKAEDEGLQVAGCVMASDAFFPFRDGIDAAAKVGITCVIHPGGSMRDQEV
                     IDAANEHGMAVVLTGMRHFRH"
     gene            complement(5598..>5769)
                     /locus_tag="PWY43_002084"
     CDS             complement(5598..>5769)
                     /locus_tag="PWY43_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL2136937.1"
                     /translation="SVKAINASRMALRRTSSPQVSLDKVIETMYETGKDMNAKYRETS
                     TGGLAIKILPCD"
BASE COUNT         1722 a         1206 c         1205 g         1636 t
ORIGIN      
        1 aaacaaatta gtgaatcttg cttctcgcaa cgccagtttt atcgctaaac gctttgaggg
       61 caaattagct gaaaaattag atgacgaagc gttatttaat gaatttaccg ctcaagcaga
      121 aaccatagcg aatttctatg aaaaccgtga atttaataaa gctattcgtg ccattatgga
      181 attaaccgat aaagccaata aatacattga cgacaaagcc ccttgggtca tcgccaaaga
      241 agaaggcaaa gaggctgaat tacaagcggt ttgttcaatg ggcattgagt tattccgtgt
      301 gctaatgagc tacctaaaac cggtattacc aaaactggca gaacgtgctg aagcattctt
      361 acaaagcgaa ttgcgttggg ataatatcgc taccccatta ttcgggcata ccgttgcacc
      421 gtttaaatcg ctgttctcac gccttgagaa aaaacagatt gatgcagtta ttgatgaaac
      481 caaagcctta tttgcagaag cgaataaaaa aaccgaaaac caaaccaaag gaacacaaaa
      541 agtggaacaa caagcagtcg aaaaacaaga aaatattgca gttgagccaa tcagccctga
      601 aattacgatt gatgatttcg ctaaattaga tttgcgtgca gccaaagtat tgaactgcga
      661 ggcagtgcca aaatcagaca aaatgttacg ttttgagctg gatttaggtg atcaccaacg
      721 ccaagtgttc tccggcatta aagcggccta cccaaatccg gaagcactta tcgggcgttt
      781 tgtgattgta attgccaacc ttgctccacg caaaatgtcg ttcggtatgt cggaaggaat
      841 gattttatct gccggcacag gcggtgcgaa cctgttctta ttagatgcag acagcggtgt
      901 tcagccggga atgcaagtta aataggagaa gatgatgatt caacaaacgc tttctattat
      961 taagccggat gctacacgcc gtaatttaat cggcaaaatt ttggctcaat tggaagaaaa
     1021 cgggctgact atcaaagctc taaaaaaagt acatcttacc cgtgagcaag cggaaggatt
     1081 ttatgcggaa cacaaaggca aagatttctt tgagccgtta gtggaattta tgacatcagg
     1141 atctattgtc gtttctgtac tggaagggga aaatgcgatc aaccattatc gtgcattaat
     1201 gggagcaacc aatccggata accgtgaaaa aggcacaatt cgtgatcttt acgccttaag
     1261 tatgcgtgaa aattcagtac acggctcaga ttcagaagag tcggcaaaac gtgaaattgc
     1321 gtacttcttt gtgccgagtg agattgttta ataaagtagg ggcgaaaaat ttttcgcccc
     1381 actattgtaa ttcgccccta gcgtagatta aatatattcc acacccacaa tgtcaaactc
     1441 gaccttaccg cccggtgtgg taatgctcac cgaatcgttc acctctttgc cgattaaacc
     1501 acgagcaatc ggggaattaa ctgaaattaa gccctcttta atattggctt catcatcacc
     1561 cacaatgcgg taagtgactt cttcatctgt atcagcattc actaactgca ccgttgcccc
     1621 aaaaatcact ttgccgttgt ttgccatttt agtgacgtca ataatttgag cattgcctaa
     1681 tttgccttca atttcttgaa tacgcccctc acagaaacct tgttgttcac gggctgcgtg
     1741 atactcggcg ttctctttta aatcgccgtg ttcacgggct tctgcaatcg catcaataat
     1801 ttgagggcga cgcacatttt ttagaaaatc caattcttca cgcagttgct ctgcaccacg
     1861 caccgtcata ggaatttgtt tcattaaatc tatttcctta ctaaaaatta tcggtttacg
     1921 aattaaaacg aaagggctat tattcaattt ctcactaaaa atttcaactc cccttttatt
     1981 atgattttta aaaatttcac tcgtgctttt ttaaataaat ttatcttatc tacacttctg
     2041 attccgagta ttacccaggc tgaaattaat accgaagaat tattaaatac cttgcctgcc
     2101 ggcacttctg ttagctttat tgctaaaaat ttagacacta accaaatcat cactcaatat
     2161 caaagtgata tttttatgct acctgccagc acccaaaaag tctttacggc attagcagcc
     2221 aaattaacct taaccgatga tttccgcttt caagctgccc ttttaagtaa cggaaaagta
     2281 gaaaacgggg tgttaaaagg cagtttaatt gcccgtttta gtggcgaccc tgagctgaca
     2341 agcggtcaaa tttatcaatt aattagcaaa ttgaagcaac aagatatcaa taaaattgaa
     2401 ggggatttaa ttttagacac ttcggttttt gccagccacg acaaagcctc cggttggatt
     2461 tggaacgact tgacgatgtg ctttaacgct ccaccagcgg caattaatgt agatcacaac
     2521 tgtttttatg tcactttaaa tgcagaccag cctattggcg aatttgctaa agtgaatgtg
     2581 ccgtctgcct acccagtgca ggtgtttagc tctgcttata ttgtcgagcc gaaagaagcc
     2641 ccattttgcc agcttgatgt agtggttcac gataacaacc gttaccaaat caaaggctgt
     2701 atggctcgcc agtctcagcc gttcggctta agtttttcgg tgcaagatcc aaccaattac
     2761 ggagcaaata tcattaaagc tcatttaaaa agcctgaaaa ttgcgtttaa cgggcaagta
     2821 aaagagtcat taaccgccca aaacggaacg ctccttgccg agcattattc cgagccgttg
     2881 ccggtgttgc tgaagaaaat gatgaaaaaa tcggataacc aaattgccga tgccctgttt
     2941 agaaccgttg ccaacaagca gcacaaccgc cctgcctcat tccaattagg cagttatgtg
     3001 attcgtcgac tattaaaaac caaagccaat attgacttta aaaacagtgt ggtgaccgat
     3061 ggctcagggc tttcccgcca taatcaagtg agtagccgaa caatgctgga aagccttgaa
     3121 tatatcgccc aaaacgaaga aagcttaaaa ctgtttgaaa ccttcccgat tgcaggtgta
     3181 gatggtacta tttccggcag aggctcaatt tcaaccgaac cgcttgccaa aaacctgatt
     3241 gctaaaaccg gctcactgaa aggggtttac aaccttgccg gctttatgaa aaatgcccgt
     3301 ggcgaacgca tcgcttttgt gcaatttatt aacggctatt ccactggcga gctggaaagc
     3361 aaaaccaaac gggctccgct taatacgttt gaaaataaat tttatatggc gttgtttaat
     3421 gaatagggct tatattttgt gtgacggatt tttgagaatt gaataagaca gcggtcgtaa
     3481 atttttgaaa ttttacgacc gcttgctcct ctctaagcaa ttaaatctcg atgacttcgt
     3541 tgcattttta cctcaatata actacaactt ggcttgattt tcagcccttt ttcttgcgca
     3601 aacgcaatta aagcattgta aagctctcct gcaatacctt taccttgaaa ggcatcatct
     3661 actcgggtac tgtaagcatc aatcacgttc tcttttatgt agcgataacg tagtgtgcct
     3721 gcttttaggt tgttttcggt aaaatactca aatacgaaat ctgctaaatt gtggttaatc
     3781 atagttcctc cgattttttt cctctattct acccgcttta atcacgagtg caagcggtcg
     3841 attttgcaga aaaatttgca aaatcgacca cttgtagatg aaaaaagcaa acgtttgcgt
     3901 tataatcttt gccgtttatt ttcacgttaa cactcatttt aaggaattta agaatggcga
     3961 ttcaacaggc gttattaagt gtgtctgata aaaaaggcat tgtcgaattt gctcgtgggc
     4021 tatcggctcg tggggtaaaa ttactttcta cgggcggaac cgcaaaatta ctggctgaag
     4081 ccggtttgct agtaacagaa gtatcagatt acaccggctt tcctgaaatg atggacgggc
     4141 gtgtaaaaac cctgcacccg aaagtgcacg gcggaattct aggtcgccgt ggtactgatg
     4201 atgaagtgat gaaccaacac ggcattgaac gaattgatat ggtggtagta aacctctacc
     4261 cttttgctca aaccgttgcc aatccaaatt gtagccttga agatgcggtg gaaaatattg
     4321 atatcggtgg tccgacaatg gtgcgttctg cagcgaaaaa tcataaagat gtggcgattg
     4381 tggtcaataa caatgatttt gatgctattc ttgccgaaat ggatcaacat caaaacagtt
     4441 taactttaga aacccgtttt aatcttgcta ttaaagcgtt tgagcatacc gcacaatacg
     4501 attcaatgat tgccaactat ttcgggcaaa aagtgccgcc ttatcaaggg gctgaggaag
     4561 aagatttaac ccaaccaagt ggacaattcc ctcgcacctt aaacctcaat tttgtgcgta
     4621 aacaaacgat gcgttatggc gaaaattcgc atcaaaacgc tgcattctat gtcgaaaatg
     4681 agttaaaaga agcctctgtt tccaccgcca aacagttaca aggtaaagcc ctttcgtata
     4741 ataatattgc agataccgat gcggctcttg aatgcgtgaa agaatttagc gagcctgctt
     4801 gtgtgattgt aaaacacgct aacccttgtg gtgttgcttt gggtaaaaat attttagatg
     4861 cctacaaccg tgcttaccaa accgatccaa cttctgcttt cggtggcatt attgccttta
     4921 accgtgaatt agacggcgaa actgctcaaa ccatcgcaga ccgtcaattt gttgaagtga
     4981 ttattgcacc aaccgtttct gcttctgcca aagaagtgtt agccactaag aaaaacgtgc
     5041 gtgtgcttga atgtggcgag ttctcagaag ctgtaaaacg cttagatttc aaacgggtaa
     5101 acggcggatt attagtgcaa gatgccgacc aaggctctgt atctattgac gatttaaagg
     5161 tggtttcaga gcgtaaacca actgaagctg agctaaaaga cctattgttc tgctggaaag
     5221 tggcgaaata tgtgaaatcg aatgcgattg tgtatgccaa agacgggcaa actatcggta
     5281 tcggtgcagg gcagatgagc cgtgtttatt ccgcaaaaat tgcaggtatt aaagcggaag
     5341 acgagggctt acaagttgct ggttgtgtaa tggcttccga tgcgttcttc ccgttccgtg
     5401 atggtattga cgctgctgca aaagtaggga ttacttgtgt gattcaccca ggtggttcaa
     5461 tgcgtgatca agaagtgatt gatgccgcca acgaacacgg tatggcagtg gtactaaccg
     5521 gaatgaggca tttccgtcat taatgttcta caaactaaaa ggctacctta ttttgataag
     5581 gtagcctttt tcattaatta atcacaaggt aaaattttga ttgctaagcc accggttgag
     5641 gtttcacggt attttgcatt catatctttg ccggtttcgt acattgtttc aatgacttta
     5701 tctaaactta cctgaggcga agaagtacgg cgtagtgcca ttctgctggc attaatcgct
     5761 ttgactgaa
//
LOCUS       DANKBV010000136         5765 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.136, whole genome shotgun sequence.
ACCESSION   DANKBV010000136 DANKBV010000000
VERSION     DANKBV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.136"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..846)
                     /locus_tag="PWY43_002085"
     CDS             complement(<1..846)
                     /locus_tag="PWY43_002085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136938.1"
                     /translation="MENLKLLTHPWAEKGLKNEIPLNREGSGVTSATATYEDGFPDVT
                     MKPIHEGGKAPSGKDMNGVLYELSTHIVHLNKGCLYPFEMDFCNAIGGYPKGSLLISN
                     DKGKLFISLIDNNTTDFNSENYFGKWDCISTQDLLNSPDGFKFIGQCESIAQLRTIEP
                     TEDQQRILVKSYYAGKNLGGGEFYADFADTTTADNAGTVIVTAGGKRWKRVYSTLNIF
                     DFGVSLDSVNNDAIKRLQAWQEPVLGLGNVIPASSRIVPASHISQVAYRFNGIDYLSE
                     DYYKAD"
     gene            complement(919..1539)
                     /locus_tag="PWY43_002086"
     CDS             complement(919..1539)
                     /locus_tag="PWY43_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HDL2136939.1"
                     /translation="MNKPHLQKTIISQYANSPIMNRIIQFADEYLETKDCFRQFYNIY
                     WDIDTAEGVGLDFWGVVLGLSRFVEINDQTTFQGSSIASTELKDKVNPTSYRMNDAMY
                     KSMLFVKAMTNIVYATAPNINKILQILFKERGRAYFVKSGTMTARYVFEFKLLDVEKA
                     IVMTLLPRPTGVLCDFYEPEQLYTWGFNENELAPFGQAAFYIGDKN"
     gene            complement(1536..2732)
                     /locus_tag="PWY43_002087"
     CDS             complement(1536..2732)
                     /locus_tag="PWY43_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL2136940.1"
                     /translation="MTIQTNVPSIQFTPQGLILPSEQEILNGVLADFNAAFGGGLSQN
                     LETPQGQLASSIAAIIADKNNQIAWLVNNLDPTYSDGIMQDAIAKIYFVKRKGRVNSF
                     ATCQFVGLPGVTIPKGLVIKDTTNNEWILDEEISILENGTVEGRVIANGVYGAKANTI
                     TKIHQSIIGLDRVTNPQDAVVGTERESRQDFAERYRESVAMNAQGMPSAVHANVMKLE
                     GVIDCYVIDNPTNKTVKHGATGYPIKPHSVYVAVRGGNDLDIAKMIWLYSGNGCDFNG
                     NTEIVVTDDTYVTPKPTYYINFMRPLDTPIFFRVKIKSGLVGDYLNEMKEAIKMQFDQ
                     SSHRKIGRIFYAMNYVSSIIKSFPKDYLLDVEVSSDGVNFENKVQLGIDQYPILFSEN
                     IILVEE"
     gene            complement(2729..3079)
                     /locus_tag="PWY43_002088"
     CDS             complement(2729..3079)
                     /locus_tag="PWY43_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136941.1"
                     /translation="MTTLFLHPQRWDLCLDDEGNIAYAKDPYAKAQDVASAIKLFKGE
                     LYYDTEKGIPYFDETLGKKQSLALYQYRLEQAALRVKGVVSAKANIESGASREVTGSV
                     TFTDEQNRQMSVKL"
     gene            complement(3083..3745)
                     /locus_tag="PWY43_002089"
     CDS             complement(3083..3745)
                     /locus_tag="PWY43_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDL2136942.1"
                     /translation="MSNNLGLLKPDQVLGGTGEFEFIISGIVNRIQTVTLVQVIAVNA
                     TGIGPVGTVDVQPMVAQLDGSGKAYAHGVIHNIPYFRLQGGSNAVIIDPEVGDIGMCG
                     FCSRDISSVKVNKAPSTPQSRRKFDYADGLYFGGFLNGVPSQYLFFKDSGIELVSTGE
                     ILLKGTSIKLDAPVETTSTVNAAGDITDNAKSGGKSMKNMRQVYDTHTHNGGPQPDRK
                     TG"
     gene            complement(3735..4622)
                     /locus_tag="PWY43_002090"
     CDS             complement(3735..4622)
                     /locus_tag="PWY43_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136943.1"
                     /translation="MSSFTKKKLKFSFVLGQQDPHFDKDENNTVIVKGLRASVTIQSG
                     HGALMPSAKLMIYGLGQKVMNKLAKIRWNTDDAKLNYVRVEASQDGGESYILVYEGVI
                     QYAYPNYGSSPEVILTIDSTSALQHKIRPVPPLSFNGEVDVASAAAQICQKMSMQFEN
                     NGVTEKVSNPYLSETALDQVFKLCDAINAKAVVEFNTIAITPQGQARDILVPVISPSS
                     GLIGYPTPTITGTRFQCLFDPVIRVQGLVEIKDSLVEICNGKWLVVGLSHYLESEVSG
                     GRWLSEVDAIYPGGVKIVK"
     gene            complement(4622..4918)
                     /locus_tag="PWY43_002091"
     CDS             complement(4622..4918)
                     /locus_tag="PWY43_002091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136944.1"
                     /translation="MDLYHIPLKAVANQRVRCQIEGQELEITLHTRLEEQLYISIKCN
                     GVAVVHNRICQDLTPLIDVGYLPMKGVLFFKDMQGRQAPHCKELGKRYRLFWGA"
     gene            complement(4921..5628)
                     /locus_tag="PWY43_002092"
     CDS             complement(4921..5628)
                     /locus_tag="PWY43_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136945.1"
                     /translation="MTWSSVGIPSVPGLPKNIGDAAIKFGGALGINLLFGNYWGVFDQ
                     NGIPLLLADNVKSVKYQSRSKISTAPLEKGSFAAYNKVIEPYTVNVVMTKGSGGVKER
                     GAFLALVDAFANSTDLFMVITPEAIYPNCNIVGYDYVREAGDGARLIKVNLHLQEIRE
                     VEVEYTKTKAESAEAVQEQGNVTPEKVNTEAGNTSVLFDIGQEIGDMKETLKTEGAGA
                     VIDKLKDGLMNAVGMGG"
BASE COUNT         1631 a         1340 c          997 g         1797 t
ORIGIN      
        1 gtccgcctta taataatcct cagacaagta atcaataccg ttaaatctat aagctacttg
       61 tgagatatgg ctagccggca caattcttga acttgccgga atcacattcc ccaaccctaa
      121 aaccggctct tgccacgctt gcaagcgctt gatggcatcg ttattcacgc tgtctaagct
      181 gactccaaag tcaaagatat tgagtgttga ataaactcgt ttccatcgtt tgccaccagc
      241 cgtcacaatc accgtgccgg cattgtctgc ggttgtggta tccgcaaaat cagcataaaa
      301 ctctccaccg cctaaatttt tcccagcgta gtaggattta accaaaatgc gttgttggtc
      361 ttctgttggc tcgatggtgc gtaattgagc aatagattca cattgaccga tgaacttaaa
      421 tccgtcagga gaattaagta aatcctgagt agaaatgcaa tcccacttcc cgaagtaatt
      481 ttcggaattg aaatcagttg tattgttgtc tatcaagcta ataaatagct ttcctttatc
      541 attgctaatt aatagtgaac cttttggata tcctcctata gcattacaaa agtccatttc
      601 aaatggataa aggcaacctt tattaaggtg gacaatatgt gtcgataact catataacac
      661 tccgttcata tccttacccg aaggtgcttt tcctccttca tgaataggct tcatagttac
      721 atccgggaaa ccatcttcgt aagtcgccgt ggcggatgta actcctgaac cttctcgatt
      781 cagtggtatt tcatttttta atcccttttc agcccaagga tgcgtgagta gctttaagtt
      841 ttccatgatt tctccaacaa aaaaccgttt atggaataag aaccataaac ggttaagtta
      901 atgaataatt taatttgttt agtttttatc gccaatataa aaagcagctt gcccgaatgg
      961 agctaactca ttttcattaa atccccaagt atataattgc tctggctcat aaaagtcaca
     1021 aagcacaccg gttggacgag gtagtaatgt cataactatt gccttttcta catccagcag
     1081 tttgaattca aaaacatacc tagccgtcat tgttcccgac ttaacaaagt aagctcgacc
     1141 tctttcttta aatagtatct gcaggatctt attgatattc ggtgctgttg catacacaat
     1201 attcgtcatt gctttaacaa aaagcattga cttatacatt gcatcattca tcctgtagct
     1261 agttgggtta actttatcct ttagctcagt cgaggctata ctggaacctt gaaaggtagt
     1321 ttggtcatta atttctacaa aacgagataa cccaagcacc actccccaaa agtcaagtcc
     1381 aacaccttca gcagtatcaa tgtcccaata aatattatag aactgcctga agcaatcttt
     1441 tgtctcaaga tattcatcag caaattgaat gatacggttc ataattggac tgtttgcata
     1501 ctgactaatg attgtttttt gtagatgagg tttattcatt cttccaccaa aattatattt
     1561 tccgaaaaaa gaatggggta ttgatctatc cccagctgaa ccttattctc aaaattaaca
     1621 ccatcactac ttacttcaac atcaagcaag taatccttag gaaaagattt aatgatcgaa
     1681 gaaacgtaat tcattgcata aaaaattcta ccaatcttgc gatgagaaga ttgatcaaat
     1741 tgcattttaa ttgcttcttt catctcattg agataatcgc caaccagccc agacttaatt
     1801 tttaccctaa agaaaattgg cgtatccaaa ggtctcatga aattgatgta atatgttggc
     1861 ttaggagtaa cgtaagtatc atcagtgact actatttcag tattaccatt aaagtcacat
     1921 ccattaccag aatataacca tatcattttt gcaatatcta aatcatttcc tcctcttaca
     1981 gcaacataaa cactatgagg ttttatagga tatccagttg ctccatgttt tactgttttg
     2041 tttgtaggat tatcaataac atagcaatca atcactcctt ctaatttcat tacattagca
     2101 tgcacagcag atggcatacc ttgagcattc atagctacgc tttctctata tcgttccgca
     2161 aaatcctgtc tgctttcccg ttctgtacca acaaccgcat cttgtgggtt ggtaacacgg
     2221 tctagcccga taatcgattg atgaattttt gtgatcgtat tagcttttgc accataaaca
     2281 ccgttagcaa taacacgtcc ttccacagtg ccgttttcta atatgctgat ctcttcatct
     2341 aatatccatt cattattggt ggtatctttg ataactaacc cttttggaat agttacaccc
     2401 ggtaaaccaa caaattgaca ggtggcaaaa gagttcactc gcccttttcg ttttacgaaa
     2461 taaattttag caatagcatc ttgcataatg ccgtcggaat aagtcggatc aagattattc
     2521 actagccacg caatttgatt atttttatca gcaataatcg cagctatcga gctggcaagt
     2581 tgtccctgtg gtgtttctaa gttttggctt agtccaccac caaatgccgc attaaagtcc
     2641 gccagcacac cgtttagaat ttcttgttcg ctcggtaaaa ttaagccttg cggtgtaaac
     2701 tgaattgatg gcacattcgt ctgtatcgtc atagtttcac gctcatctgt ctattttgtt
     2761 catcggtaaa agtcacagag ccggtcactt cccgactggc tccgctttca atatttgctt
     2821 tggcagaaac cactccttta actctgaggg ctgcttgctc taagcgatat tgatataatg
     2881 ctagagattg cttcttccca agtgtttcat caaaataagg aatccctttc tccgtgtcat
     2941 aatacaactc gcctttaaac agctttatgg cacttgccac atcttgagct ttagcataag
     3001 gatcttttgc ataagcgata ttcccctcat catcaaggca taaatcccac ctttgcgggt
     3061 gtaaaaataa cgttgtcata cattaccccg ttttccggtc aggttgtggc ccaccattgt
     3121 gcgtgtgcgt atcataaacc tgtcgcatat ttttcattga tttacctcct gatttggcat
     3181 tatcggtaat atctccggca gcgtttactg ttgaggtagt ttcaacaggg gcatctaatt
     3241 tgattgatgt gccttttagc aaaatttcac cggtagaaac caactcaatg cccgaatctt
     3301 tgaaaaagag atattggctc ggcacaccgt taagaaaacc gccaaaatac aagccatctg
     3361 cataatcaaa ttttcgtctg ctttgtgggg tcgagggggc tttgttcact ttcactgagg
     3421 aaatatcacg actgcaaaaa ccacacattc caatatcgcc tacttccgga tcgataataa
     3481 ccgcattgct gccaccctga agtctaaaat aagggatatt gtggattaca ccgtgtgcat
     3541 acgctttgcc actaccgtcg agttgtgcca ccattggttg cacatctacc gtgccgaccg
     3601 gtccgatgcc ggttgcattt accgcaatca cttgcactaa tgtcactgtt tgaattcgat
     3661 tcacaatgcc ggaaataata aattcaaatt cgcctgtgcc acctaaaact tgatcgggct
     3721 ttagtaaacc taaattattt gacaattttc acacctccgg ggtaaatggc atcgacttct
     3781 gataaccaac gccctcctga tacttcactt tctaaataat ggcttaatcc aacaaccagc
     3841 catttgccat tacaaatttc aactaaactg tctttaattt ccacaagccc ttggacgcgt
     3901 ataacaggat caaataaaca ttgaaacctt gtgccggtaa tggttggggt tggatatcca
     3961 attaatccgc tactcggcga aataacggga actaaaatat ctcttgcttg accttgcggc
     4021 gtgatagcaa tcgtattaaa ttccacgacc gcttttgcat tgatggcatc acataattta
     4081 aaaacttgat ctaaagcggt ttcagacaag taagggttgc tcactttctc tgttacacca
     4141 ttattctcaa attgcatact cattttttgg caaatttgag cggccgctga agccacatca
     4201 acttcaccgt taaaacttaa tggcggaacc ggtctaattt tgtgttgtaa agctgatgta
     4261 ctatcaatcg ttaagatcac ctcagggctt gaaccataat tcggataggc atattgaata
     4321 acgccctcat aaaccaatat atagctttct cccccatctt ggcttgcctc tactctgacg
     4381 taattcagct tagcgtcatc agtattccag cggattttcg ccaatttatt cattaccttt
     4441 tgaccaagcc cgtatatcat tagctttgca ctcggcatta gtgcaccgtg tccgctttga
     4501 attgtgacac tcgccctcaa tcccttaaca atcaccgtat tattttcatc tttgtcaaaa
     4561 tgcggatctt gctgtccaag cacaaaagaa aacttcagct ttttcttggt aaaacttgac
     4621 attacgcccc ccaaaacaag cggtatcttt tgcctaattc tttgcaatgc ggagcttgcc
     4681 gcccttgcat atctttaaaa aacaaaacgc ctttcatcgg caggtaacct acatcaatta
     4741 aaggcgttaa atcttgacaa attcggttat gcaccaccgc tacaccgttg cattttatgc
     4801 tgatataaag ctgttcttcc aaacgggtat gcaaggtgat ttcgagttct tgtccctcaa
     4861 tttggcagcg tactcgttga tttgccaccg ctttaagtgg aatatgatat aagtccaaaa
     4921 tcaacctccc attccaacgg cgttcatcaa gccatctttc agtttatcaa taactgcacc
     4981 ggccccctcg gtttttaatg tttctttcat atcaccgatc tcttgtccta tatcaaaaag
     5041 gactgaagta ttgccggctt cagtatttac tttctctggc gttacattcc cttgctcttg
     5101 cacagcttct gccgattctg ctttagtttt cgtgtattcc acctctacct ctcgaatttc
     5161 ctgcaggtgt aaatttactt taatcaacct tgcaccgtct ccggcttcac gcacatagtc
     5221 ataaccaacg atattgcagt tcgggtaaat agcttcagga gtgatcacca taaataaatc
     5281 agtgctgttg gcaaaggcat cgacaagggc taaaaatgcc cctcgttctt tcacaccgcc
     5341 actgcctttt gtcattacta cattcacggt ataaggctcg atcactttgt tgtaagcggc
     5401 aaatgagcct ttttcgagtg gtgcggtaga aatctttgaa cggctctgat atttcaccga
     5461 tttcacatta tccgcaagta aaagaggaat gccgttttga tcgaacactc cccaataatt
     5521 gccaaataat aaattgatac ccaacgcacc accgaatttt attgccgcat cgccgatatt
     5581 tttcggtaac cccggcacac tcggtatgcc gacagaactc caagtcatat tttctccaaa
     5641 aaaataaccg cttgcggaac aagcggttgg gttaattaac gttgagctgt acaaattttt
     5701 ccgtcgcagt cattgcctaa ctctaagtag tcagcagaga tagcaaaggc ggttaaaaat
     5761 agaat
//
LOCUS       DANKBV010000137         5667 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.137, whole genome shotgun sequence.
ACCESSION   DANKBV010000137 DANKBV010000000
VERSION     DANKBV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.137"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            12..824
                     /locus_tag="PWY43_002093"
     CDS             12..824
                     /locus_tag="PWY43_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2136946.1"
                     /translation="MGRPELERAVLSVQNQTYPCKHYVFVDGEKYHQQAADILKNFPS
                     VIVTYLPMNTGADGWVNSSINAIALFLVKEDIICYLDDDNWFEPTHVESGVHALLEMQ
                     SDYAYSLRNFYDLDGEFLCIDTIESLGEYNCVYQEPLECNYQLGGVKGVLSINLNDCQ
                     HIDTNCYFIRSEIARLISTAWYTGIHNDRNVYKKLQELALKGNCTKKVTVNYTLDTRK
                     YYGSVFNELLEEPFLLSEYDAYQFTNEVIKFHSEESLKAWGGCLIWEQNNGS"
     gene            956..1654
                     /gene="aphA"
                     /locus_tag="PWY43_002094"
     CDS             956..1654
                     /gene="aphA"
                     /locus_tag="PWY43_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDL2136947.1"
                     /translation="MTAATMFASLIAFSAQAGGPKVAYTQPGIDMRTHEAQQAPIHWV
                     SIEQIKESIKDLPPMAVSFDIDDTVAATSGCFHYGKQKYSPDDFSYLKNQDFWDEINA
                     GCDKYSLPKQVAINLIKMHQERGDQVYLITGRTAGKNEQVTTIMQKYLGIKNMQPVNF
                     MGGKAHSTKYDKTPAIIEHKVQIHYGDSDDDILAAREAGIRGIRILRAANSNYTPFPQ
                     AGGYGEEVVVNSSY"
     gene            1835..2596
                     /gene="udp"
                     /locus_tag="PWY43_002095"
     CDS             1835..2596
                     /gene="udp"
                     /locus_tag="PWY43_002095"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDL2136948.1"
                     /translation="MSEVFHLGLTKAMLKGAKIAIVPGDPARSERIAKKMDNPEFLAS
                     TREFTSWLGYLDGEPVVVCSTGIGGPSVSICVEELAQLGVRTFLRIGTTGAIQEHINV
                     GDILITTGAVRLDGASQHFAPMEYPAVANFECTNALYAAAVESGVTPYVGITASSDTF
                     YPGQERYDTFTGKIYRHFQGSLKQWQDLNVMNFEMESATLFTMCSALGLRAGMVAGVI
                     VNRTQQEIPNEATIHDTEHKAVSVVVEAARKLIKG"
     gene            2671..3267
                     /locus_tag="PWY43_002096"
     CDS             2671..3267
                     /locus_tag="PWY43_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136949.1"
                     /translation="MKKWLMLITLIFGITACFSSLEFDESKQEKINSFLIVGNKLYVV
                     GELHDYEFEHQQVAELNNFLKSLIANKIKSVEANFEVKESKKVEGSYRIILDGKSLTE
                     SERSTVMDTFHFNSAQNPSITYQAEGNLVKLANREELLQKFRFKQALEANVRYYKMKS
                     GLQNTAEGALSIVMLPVAVVAVLPFYMFWVGACATYGC"
     gene            3278..3763
                     /locus_tag="PWY43_002097"
     CDS             3278..3763
                     /locus_tag="PWY43_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDL2136950.1"
                     /translation="MYSLYLKILSECDWCCDPKTLSTYQQEWLLHEGSLTQKLYDVAT
                     NFNVEVVQEKWIAKNSEKTTACNEDVWSREVLLKDGEKNWIFAQTLVPRSTIENIAQN
                     VLELGNQPIGLWLFSQNPTRTKLEWTQTENGLFARRGVYEIRGYSLEIRELFLETFPY
                     L"
     gene            complement(3801..4670)
                     /locus_tag="PWY43_002098"
     CDS             complement(3801..4670)
                     /locus_tag="PWY43_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2136951.1"
                     /translation="MKNINTQAVGWIGLGQMGEPMAKHLLNSGVNVGVYNRDPAKAEK
                     VVEAGGTLYPSVLELVKAYDVIFLMIADYAAVQQVLSDEVLAELNGKVVVNMSTISPS
                     QNQAVEALLAQHGAEFVEAPVSGSSKVAEAGKLLVLAAGKEETVELLKPLFAAFSTTT
                     FYYGEVGKAGGIKLMINSLLGIFIQAYGEALNFADQYGIPKEKVIEMISGSFMNSPIF
                     QAKVPMYQANEFPPAFMMKHMTKDFNLASEEIAKLGKSYPLIEQATKTYNQANDSGLS
                     EVDMAAIYQFLAK"
     gene            4800..5591
                     /locus_tag="PWY43_002099"
     CDS             4800..5591
                     /locus_tag="PWY43_002099"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL2136952.1"
                     /translation="MKPTILLYDSGMGGLTIYDEIRKVLPHAHYLYCFDNAFFPYSEK
                     SEAVLIERAVKIVQKIAEKQPLDMVVVACNTASTVVLPALREHFNFPIVGTVPAIKPA
                     AAISETKVIGLLATKGTVARPYVDELIRKYAQDCTVEKIGTTDLVEIVEEKQRSGKVD
                     MNRLQKVVEAWQGKPELDTVILGCTHFPLVKAELQQLLPDVKYFVDPGVGIAKRVLSL
                     LEERKFQQKADFENQAFCTKQSSAFNIKQDVMQNWGFNNLSVIDI"
BASE COUNT         1764 a          990 c         1179 g         1734 t
ORIGIN      
        1 tcagttcaac aattggtcgt cctgaattag aaagagctgt tttaagcgta caaaatcaga
       61 cttatccttg taaacattat gtttttgttg atggagagaa atatcaccaa caagcggctg
      121 atattttaaa gaattttcca agtgttattg ttacttatct cccaatgaat accggggctg
      181 atggctgggt aaatagcagc attaatgcaa ttgcactatt tttagtgaaa gaagatatta
      241 tttgttatct ggatgatgat aattggtttg aacctactca cgttgaatct ggtgtgcatg
      301 ctttattaga aatgcaatct gattatgctt actctttacg taatttttat gatttagatg
      361 gcgagttttt gtgtattgat acgattgaat cgttgggaga atataattgc gtttatcaag
      421 aaccattaga atgtaattat caattaggtg gtgttaaagg tgttttatca ataaatctga
      481 atgattgtca gcatattgat acgaattgct attttattcg tagtgagata gctcgcctta
      541 tcagtacggc ttggtacaca ggtattcata atgatagaaa tgtatataaa aaattacaag
      601 aattggcact aaaaggaaat tgtacaaaga aagttacggt taattatact cttgatacac
      661 gtaaatatta tggatcagta tttaatgaat tattagagga gccattttta ttaagcgaat
      721 atgatgccta tcaatttacg aatgaggtga ttaaatttca ttcagaagaa agccttaaag
      781 cttggggagg ttgcttgatt tgggaacaga acaatggctc ataagcgtta agcgtaatcg
      841 tttgcttgtg atcatattca caaaataagg tttttgtatt agctaagagt gcctaataag
      901 attaaaatag gtatggttaa tcatatggag gtattttata aaatttacat taaaaatgac
      961 ggcagcgaca atgtttgcat ctcttattgc tttttcagca caagcaggcg gaccaaaagt
     1021 agcttatacc cagccgggaa ttgatatgcg aacacatgaa gcacagcaag cgccaattca
     1081 ttgggtatct attgagcaaa ttaaagaaag catcaaagat ttacccccta tggcggtaag
     1141 ttttgatatt gatgatactg ttgctgcaac tagcggttgc ttccactacg gaaagcaaaa
     1201 atattcgcca gacgatttca gctacttaaa aaatcaagat ttttgggacg aaattaatgc
     1261 tggctgtgat aaatattctt tgccaaagca agttgctatt aatttaataa aaatgcacca
     1321 agaacgtggt gatcaagttt atttaattac ggggcgtacc gcaggtaaaa atgaacaagt
     1381 tacaacgatt atgcaaaagt atcttggtat taaaaatatg caaccggtaa attttatggg
     1441 ggggaaagca cattcaacaa aatatgataa aacccctgcg attattgaac ataaggtgca
     1501 aattcactat ggtgacagtg atgatgatat cttagctgcc cgtgaagccg gtattcgagg
     1561 tattcgtata cttcgtgcag caaattctaa ttatacgcca ttccctcagg ccggaggata
     1621 tggtgaagaa gttgttgtca attcaagtta ttaatttctt agtctgaaat atggccacca
     1681 ttgcgtggcc atcatttttt atttatataa tgacgaaacc gtttaccttt gatctagttc
     1741 acaaaatcaa actttgcttg caaaaaattg tcgtaaatta accgcttgct aagatagaat
     1801 ggctcatatc cagttagata aaaaggagat aaaaatgtct gaagtatttc atttaggttt
     1861 aaccaaagcc atgctcaaag gtgcaaaaat tgcaatcgtt ccgggcgatc cggctagaag
     1921 tgaacgtatt gccaaaaaaa tggacaaccc agagttttta gcctcaaccc gtgaatttac
     1981 ctcttggtta ggttatttag atggcgaacc ggtggtggtt tgttcaaccg gtattggcgg
     2041 accgtcggtg tcgatttgtg tggaagagtt agcacagtta ggtgttcgca cattcttacg
     2101 cattggcaca acaggtgcaa ttcaagagca tattaatgta ggcgatattt taattaccac
     2161 cggagcggtg cgtttagatg gtgcgagcca acattttgca ccaatggaat atcctgcagt
     2221 agcgaatttt gaatgtacta atgctcttta tgcagcagca gtggaatcag gcgttacacc
     2281 ttatgtgggg attacagctt catcagacac tttctacccg gggcaagaac gttacgacac
     2341 tttcaccggc aagatctatc gtcatttcca aggttcatta aaacagtggc aagatctcaa
     2401 tgttatgaat tttgaaatgg aatccgccac tctctttacg atgtgttcgg cattaggctt
     2461 gcgagcaggt atggttgctg gtgtaattgt aaaccgcact caacaagaga ttccgaatga
     2521 agcaaccatt cacgacaccg aacataaagc cgtttcggta gtagtggaag cggcaagaaa
     2581 attaattaaa ggctaatctt taaatttaaa agcaaaaaag gtaaattgtc tttgttacaa
     2641 tttacctttt ttctttttag ggagctttct atgaaaaaat ggttgatgtt aattacatta
     2701 attttcggca ttaccgcttg tttttccagt ttagaatttg atgaaagtaa acaagaaaaa
     2761 atcaattcat tcttgattgt tggtaataaa ttatatgtgg tgggtgaact gcacgattat
     2821 gaatttgagc atcagcaagt tgcggaactg aataattttc tcaaaagcct aattgctaat
     2881 aaaatcaaat ctgttgaggc aaacttcgag gttaaggaat ccaaaaaagt ggaaggtagt
     2941 tatagaatta ttttagatgg gaaatcactt acagaaagtg aaagaagtac ggtgatggat
     3001 acctttcatt ttaattcagc tcaaaatcca tctattactt atcaggcgga agggaacttg
     3061 gtgaaattag ccaatagaga agagcttttg caaaaattcc gatttaaaca agcgcttgag
     3121 gcgaatgtgc gttattacaa aatgaaaagt ggtcttcaaa atacggctga aggagcgtta
     3181 agtattgtaa tgttgcctgt tgctgtagta gctgttttac cattctatat gttttgggtt
     3241 ggtgcttgtg ccacttacgg atgttaaata actttttatg tattctctat acctgaaaat
     3301 tttatcggaa tgcgattggt gttgtgatcc aaaaacactt tctacctacc agcaagaatg
     3361 gcttctgcac gaagggtcac ttacccaaaa attgtatgat gtcgcaacca atttcaacgt
     3421 ggaagtagta caagaaaagt ggattgcaaa aaacagtgaa aaaacgaccg cttgtaatga
     3481 agatgtttgg tcgcgagaag tgttactgaa agatggtgaa aaaaattgga tttttgccca
     3541 aactcttgtg ccaagatcaa caatcgaaaa tatcgctcaa aacgtgttgg aattgggcaa
     3601 tcagccgatt ggtttatggc tattttctca aaatcctact cgcaccaaat tagaatggac
     3661 acaaacagag aacggtttat tcgctcggcg tggggtgtat gaaattagag ggtattctct
     3721 ggaaattcga gagctttttt tagaaacctt tccttattta taaaaaaatc ggttggtaat
     3781 gacaccaacc gattttcatc ttattttgcc aagaactgat aaattgcagc catatccact
     3841 tcgctcaagc ctgaatcatt ggcttgattg taggttttag tggcttgctc aataagcggg
     3901 taagatttgc ctaatttcgc aatttcttcg cttgccaaat taaaatcttt ggtcatatgt
     3961 ttcatcataa acgcaggcgg aaattcatta gcttggtaca tcggcacttt tgcttggaag
     4021 atcgggctgt tcataaacga gcctgaaatc atttcgatta ctttctcttt cggaatgcca
     4081 tattggtcgg cgaagtttaa cgcctcgcca taggcttgaa tgaagatgcc cagtagcgag
     4141 ttgatcatca atttaatccc accggcttta cccacttcac cataatagaa agtggtggta
     4201 ctaaaggcag cgaaaagcgg tttgagcaac tcaacggttt cttctttacc ggcagccagt
     4261 accaatagtt tacctgcttc tgccactttg cttgaaccgg aaaccggagc ttcaacaaat
     4321 tctgcaccgt gttgggctag tagagcttct accgcctggt tttggcttgg ggaaatggtg
     4381 ctcatattca ccactacttt gccgtttaat tcagccaaca cttcatcgct taacacctgt
     4441 tgcacggcgg catagtctgc aatcatcaag aaaatcacat cgtaggcttt caccaattcc
     4501 aacacagaag gatataatgt gccgcctgct tcaactacct tttctgcttt tgctgggtcg
     4561 cggttgtaaa cgcccacgtt cactccgcta tttagcaaat gttttgccat cggttcgccc
     4621 atttgcccta aaccaatcca accgactgct tgtgtattaa tgtttttcat tatttttctc
     4681 cttactgaat aaagcaaatt gattattgcg attttccctc atcaagtcaa atagagagaa
     4741 cttgaatata gcaatggaaa agagtagcat tttagcactt acttttttga acaataccaa
     4801 tgaaaccaac aattctttta tacgattccg gaatgggcgg attgacgatt tacgatgaaa
     4861 tccgaaaagt gttaccgcac gctcactatt tatattgctt tgataacgct tttttcccat
     4921 attccgagaa gtcggaagca gttttaattg agcgagcggt caaaattgtg caaaaaattg
     4981 cagaaaaaca accgcttgat atggttgttg ttgcttgtaa tacggcaagc acagtggttc
     5041 tgccggcatt gcgtgagcat tttaattttc cgatagtagg aactgtaccg gcaattaaac
     5101 cggcagcggc aatttccgaa actaaagtca tcggcttgct cgccactaaa ggcacggttg
     5161 ctcgccctta tgttgatgaa ttgataagaa aatatgctca ggattgtacg gtagaaaaaa
     5221 ttggcacaac cgatttggtt gaaattgttg aagaaaaaca acgaagcggt aaagtggata
     5281 tgaaccgact acaaaaagtg gtcgaggctt ggcaaggtaa gccggaattg gatacggtta
     5341 tactcggttg tacccatttt cccttagtca aagctgaatt acagcaatta ttgcccgatg
     5401 tgaaatattt tgttgatccg ggtgtaggta ttgccaaacg ggtgctaagc cttttggaag
     5461 aacgcaaatt tcaacaaaaa gccgattttg aaaatcaagc attctgcact aaacaaagtt
     5521 ctgcatttaa tatcaaacaa gacgttatgc agaattgggg atttaataat ctttcggtga
     5581 ttgacattta aacaaaacaa tctgttttga tgaaaaaagg ttcagttgag caaaaacttt
     5641 aaaaaagggc ttgcactcaa ttttaaa
//
LOCUS       DANKBV010000138         5654 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.138, whole genome shotgun sequence.
ACCESSION   DANKBV010000138 DANKBV010000000
VERSION     DANKBV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.138"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..733
                     /locus_tag="PWY43_002100"
     CDS             <1..733
                     /locus_tag="PWY43_002100"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDL2136953.1"
                     /translation="VKKEIQAGNSYLLNLSYPTAIETNYSLAEIFAASKAKYKCYLQG
                     RFVCFSPECFVRIENNRIFSYPMKGTINANEENAEQKLMASEKEFTEHNTIVDLIRND
                     LALVAKNIEVTKYRYVEKVETHRGAILQTSSEICGELGENWQENIGSILAKLLPAGSI
                     SGAPKEKTVSIIQQAELGKRGYYTGIFGYFDGESLESAVAIRYIAQTSNGLVFHSGGG
                     ITSQSVLTDEYNELLEKVYVPISAI"
     gene            711..1331
                     /locus_tag="PWY43_002101"
     CDS             711..1331
                     /locus_tag="PWY43_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDL2136954.1"
                     /translation="MCRFPLFETLSIIDGKVQNLQYHQQRFEQATQEYFGCEPRFSLA
                     DILVVPEASQRGKVRCRIDYNATEFELKFFIYSPKKVERFRLVEVENWDYHLKFSDRK
                     RFDLLPILQNEEVIILNNGYVSDCSIGNLLFLKQGIWYSPQHYLLKGTQLSKLLDEGK
                     VVLAKIAKQDLHQYEKIMLINALNPFEEPRALPCSQIYFCDLDRKS"
     gene            1432..2538
                     /gene="recA"
                     /locus_tag="PWY43_002102"
     CDS             1432..2538
                     /gene="recA"
                     /locus_tag="PWY43_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL2136955.1"
                     /translation="MAEKKSQKNTPVKQIDPEQKEKALAAALAQIEKQFGKGSIMKLG
                     DTQALDIEAVSTGSLGLDSALGIGGLPMGRIVEIYGPESSGKTTLTLSVVAQAQKNGK
                     TCAFIDAEHALDPIYARKLGVDTDGLLISQPDNGEQALEICDALVRSGAVDVIIVDSV
                     AALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKATNCLVIFINQIRMKIGVMFG
                     NPETTTGGNALKFYASVRLDIRRSGVVKDGDEVIGSETKVKIVKNKVAPPFREVQFDI
                     MYGEGIARMNELLILAESHGFINKAGAWFSYEGEKIGQGKNNAIKWLKEHPEVASKIE
                     QDIRNLLISNPTFTATPDSENADNADDEFSEEEL"
     gene            2597..3073
                     /gene="recX"
                     /locus_tag="PWY43_002103"
     CDS             2597..3073
                     /gene="recX"
                     /locus_tag="PWY43_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL2136956.1"
                     /translation="MTTSTTKTNKYTAVNYLLYLLSKRDYSEQELRQKLKQKEYELTE
                     IDAAIEKAQANQWQSDERFCTTFIRYRSMQGIGPRRLKQELKLKGVKDWLINQELENA
                     EVDWFELAEQIFEKKRPLVWDLKAKQKMWRFMVSRGFYNDHFSHLMALDYSSDEYE"
     gene            3066..3377
                     /locus_tag="PWY43_002104"
     CDS             3066..3377
                     /locus_tag="PWY43_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL2136957.1"
                     /translation="MNREKITFFSRFEADILSGKKTITIRDKSESYFQPNQELAVFTN
                     ETDRFFANIKVLSVTPIVFDELNEQHAKQENMSLAELKQVIREIYPNDNAFFVIEFTL
                     I"
     gene            3387..3923
                     /locus_tag="PWY43_002105"
     CDS             3387..3923
                     /locus_tag="PWY43_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDL2136958.1"
                     /translation="MVLFKSTKQTETCPQCGSPLQIKQGKKGLFLGCSAYPECDYIKP
                     LHQAGHIIKQLDELCPECGGNLQLKQGNYGIFIGCSHYPECHFIVHEEAEVEEEFDCP
                     ECKQHKLIARKGRSGKIFYGCIGFPNCKFTLATRPINHLCEKCGCELATIKKIKGKQF
                     TICANKSCQHQTELKPNE"
     gene            3916..4470
                     /locus_tag="PWY43_002106"
     CDS             3916..4470
                     /locus_tag="PWY43_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDL2136959.1"
                     /translation="MNNFLEIVNQLKQEEVVAYPTEAVFGLGCNPQSEKAVRKLLVLK
                     QRPEEKGLILLASDLKYLLPYIDEARMTEQAWQRLSHIGEQAITWIVPVKNTVPSYLT
                     GKFDSIAVRLCNVPAVVQLCEASGVALTSTSANLSGEPPCRNAKEVIDQFGQDFPVLH
                     ADTLGRENPSEIRDIFSQQIFRQG"
     gene            4477..5301
                     /gene="aroE"
                     /locus_tag="PWY43_002107"
     CDS             4477..5301
                     /gene="aroE"
                     /locus_tag="PWY43_002107"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL2136960.1"
                     /translation="MNRYAVWGNPIAQSKSPRIHQLFALQTQRNIEYIANLGDETAFE
                     QQLADFFASGAKGCNITAPFKERAFKLADEFSETALLAQACNTLKRLEDGRLYADNTD
                     GAGLVSDLERLGWLQKGHKTLILGAGGATKGVLLPLLKAGLEITLYNRTQEKAVVLAE
                     KFAKFGKIQTACLSDLANQRFNLIVNATSLGLQGKYVELPESVIASANIYDMQYAPNM
                     QTPFLNYARRCGATACQDGLGMLVGQAAFAFSLWEGQFPDVEPVLAQLKLEMQTVK"
     gene            5468..5544
                     /locus_tag="PWY43_002108"
     tRNA            5468..5544
                     /locus_tag="PWY43_002108"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5502..5504,aa:Met,seq:cat)
     gene            5547..5631
                     /locus_tag="PWY43_002109"
     tRNA            5547..5631
                     /locus_tag="PWY43_002109"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5581..5583,aa:Leu,seq:tag)
BASE COUNT         1822 a         1037 c         1222 g         1573 t
ORIGIN      
        1 agtgaaaaag gaaattcaag ccggcaatag ctacttgctg aatttgagct acccgactgc
       61 cattgaaacc aattattcat tggcggaaat ttttgccgcc agcaaagcaa aatataaatg
      121 ttatttgcaa ggcaggttcg tctgtttttc ccctgaatgc tttgtgcgaa ttgaaaataa
      181 ccgcattttt tcctacccga tgaaaggcac aattaatgca aatgaggaga atgccgagca
      241 gaaattaatg gcttcggaaa aagagtttac cgaacataat accattgtgg atttgatccg
      301 aaatgacttg gcgttggtgg cgaaaaatat tgaagtgact aaataccgtt atgtggaaaa
      361 agtagaaacc catcgagggg caattttgca gacaagttcc gagatctgtg gcgaattagg
      421 tgaaaattgg caagaaaata tcggttcgat attggcaaaa ttactacctg ccggctccat
      481 tagcggtgca ccgaaagaga aaacggtttc gattattcag caagcagaat taggaaaacg
      541 gggttactac accggtattt ttggttattt tgatggcgag agcttagaaa gtgcggtggc
      601 aattcgttac attgctcaaa cctcaaacgg gcttgtgttt catagtggtg gtggcattac
      661 ctcacaaagt gtgttaactg atgaatataa tgaactgtta gagaaagtct atgtgccgat
      721 ttccgctatt tgaaaccctt tcgattattg acggcaaagt tcagaactta cagtatcatc
      781 aacaacgttt tgagcaagca acacaagaat attttggctg tgaacctcgt tttagccttg
      841 ctgatatttt agttgtgcca gaggcttctc aacggggcaa agtacgttgc cgaatagatt
      901 acaacgcaac agaatttgag ctgaaatttt ttatctattc gcccaaaaaa gtagaacgtt
      961 ttcgcttggt tgaggtggaa aattgggatt atcacctcaa atttagcgac cgcaagcggt
     1021 ttgatttgct ccctatttta caaaatgaag aggtgattat tctcaataat ggctacgtca
     1081 gtgattgctc catcggcaat ttactgttcc taaaacaagg aatctggtat agcccgcaac
     1141 attatttgct caaaggcact cagcttagta aattgcttga tgaagggaaa gtcgttttag
     1201 ccaaaatagc caagcaagat ttgcatcaat atgaaaaaat tatgttgatt aatgctctta
     1261 atccctttga ggaaccaagg gcattgcctt gctcgcaaat ttatttttgt gatcttgatc
     1321 gaaaatccta aaaaaatgct tgcaactgga taagtaaata ctataataat atacagttat
     1381 tttgcagcaa caagcggttg cttttagata aaagtttaca aaggatagaa tatggcagag
     1441 aaaaaaagtc aaaagaacac tccggttaaa cagattgatc cggaacaaaa agaaaaagcg
     1501 ttggcagccg cattagctca aattgaaaag caatttggca aaggctcaat tatgaagtta
     1561 ggcgatactc aagcccttga tattgaagca gtttcaaccg gttctcttgg tttagactca
     1621 gcattaggca ttggtggttt accaatgggt cgtattgtgg aaatttatgg accggagtct
     1681 tcaggtaaaa ctacattaac tttatctgtg gtggctcaag cccaaaaaaa tggtaaaact
     1741 tgtgccttta ttgatgccga acacgcactt gatcctattt acgcccgtaa gttaggggta
     1801 gatacagatg gattattaat ttcacaaccg gataatggcg aacaagcatt agaaatttgt
     1861 gatgcgttag ttcgttccgg tgcggttgat gtcattattg tggactctgt tgcggcactt
     1921 acaccaaaag cagagattga aggcgatatg ggtgattccc atatgggctt acaagctcgt
     1981 ttaatgtcac aagcattgcg taaattaacc gcaaatatca aagcgaccaa ctgcttggtg
     2041 attttcatta accaaattcg tatgaaaatt ggggtaatgt tcggtaaccc tgaaaccaca
     2101 actggtggta atgcccttaa attctatgct tcagtacgtt tagatattcg tcgttcaggc
     2161 gtagtaaaag acggtgatga ggttatcggt agcgaaacaa aagtgaaaat tgtgaaaaac
     2221 aaggttgctc caccgttccg tgaagtccaa tttgatatta tgtacggtga gggcattgcc
     2281 cgtatgaatg aattactcat tttagcggaa tctcacggct tcattaataa agcaggggct
     2341 tggttctctt atgaagggga gaaaatcggt cagggtaaaa ataatgccat caaatggtta
     2401 aaagaacacc ctgaagtggc gagtaaaatt gagcaagata ttcgaaatct actcatttca
     2461 aacccaactt ttacggctac tccagattct gaaaatgcag acaatgcaga tgatgaattt
     2521 agtgaagaag aactctaatt aaatttatgc ggttttcgct caacgtgaaa atcgcatttt
     2581 tatttataac cgataaatga caacatctac tactaaaaca aataaataca cagcggttaa
     2641 ttatctgcta tatttacttt caaagcgtga ttacagcgag caagagcttc ggcaaaaact
     2701 aaagcaaaaa gagtatgaac tcacggagat tgatgcagcg attgaaaaag ctcaagcgaa
     2761 tcagtggcaa agtgatgaac gcttttgtac cacttttatc cgttatcgtt caatgcaagg
     2821 cattgggcct cgccgtttaa agcaagagct aaaacttaaa ggggtaaaag attggttaat
     2881 taaccaagaa ttagaaaacg ccgaagtgga ttggtttgaa ttagctgagc aaatttttga
     2941 gaaaaaacga ccgcttgtat gggatctgaa agcaaagcag aaaatgtggc gatttatggt
     3001 tagccgtggt ttttataacg atcattttag tcatttaatg gctctggatt acagtagcga
     3061 tgaatatgaa tagagagaaa attacctttt tcagccgatt tgaagccgat attttaagcg
     3121 gtaaaaaaac aattacgatt cgagacaagt cagaatctta ttttcaacca aaccaagaat
     3181 tagcggtttt tactaatgaa accgatagat ttttcgccaa tattaaagta ctatcggtaa
     3241 cacctatagt gtttgatgaa ttaaacgagc aacacgctaa acaagaaaat atgagcttag
     3301 ccgaattgaa acaagttatt cgggagattt atccgaatga caatgcgttt tttgtgattg
     3361 aatttacgct tatttagcaa atcaatatgg tacttttcaa atcaaccaaa cagaccgaaa
     3421 cttgcccaca atgtggctcg ccgttgcaaa ttaagcaggg caaaaaaggg ttatttttgg
     3481 gttgttcagc ttaccctgaa tgtgattata ttaagccatt acatcaagcc ggtcatatta
     3541 ttaagcaatt agatgaactt tgccctgaat gtggcggaaa cctccaatta aaacaaggca
     3601 attatggtat ttttatcggc tgtagccatt accctgaatg ccacttcatc gtacacgaag
     3661 aagctgaggt tgaagaagag tttgattgcc ctgaatgcaa gcaacataaa ctgattgcta
     3721 gaaaagggcg ttcaggcaaa atattttatg gttgtatagg cttccctaat tgcaaattta
     3781 cattagcaac tcgaccgatt aaccacttgt gtgaaaaatg tggttgtgaa ctcgctacaa
     3841 tcaaaaaaat taagggcaag caattcacca tttgtgctaa caaaagttgc cagcaccaaa
     3901 cagaattaaa accgaatgaa taatttttta gaaatagtta atcaattaaa gcaagaagaa
     3961 gttgtagctt acccaaccga agcggttttt ggcttaggct gcaatccaca aagtgaaaaa
     4021 gcggtaagga aattactcgt attaaaacaa cgccctgagg aaaaagggct aattttactg
     4081 gcttctgatt taaagtatct tcttccctat attgatgaag ctcgaatgac ggaacaggct
     4141 tggcaacgcc taagccatat tggcgagcag gcaattactt ggatcgtacc ggttaaaaat
     4201 actgtgccaa gttacctcac tggcaaattt gattctattg ccgtacgttt atgtaatgtg
     4261 ccggcggtgg ttcagttgtg tgaggcaagc ggcgttgcgt tgacctctac cagtgccaat
     4321 ttgagcggag aaccaccttg ccgcaatgca aaagaagtca tcgatcagtt tggtcaagat
     4381 tttcctgtat tacacgcaga cacgctaggt agagaaaacc cttccgaaat tcgagatatt
     4441 ttcagccaac agatttttag acaaggataa gccgaaatga accgatatgc cgtgtggggc
     4501 aacccgattg ctcaaagtaa atccccccgt attcatcaac tctttgcttt acaaacccaa
     4561 cgcaacattg aatatatcgc taacttagga gatgaaacgg cttttgagca acaattagcc
     4621 gattttttcg cttctggagc aaaaggttgc aacatcaccg ccccttttaa agaaagagcc
     4681 tttaaattgg cagatgaatt tagcgaaacc gctttgttgg cacaggcgtg taatacgctt
     4741 aaacgcttag aagatggacg gttatatgcc gataataccg acggggccgg tttagtttct
     4801 gatttggaac ggctaggttg gttacaaaaa gggcataaaa ccttaattct tggagcaggt
     4861 ggagcaacca aaggcgtatt acttccgtta cttaaagcgg ggttagagat tactctatat
     4921 aaccgcacgc aggagaaagc ggtcgtttta gccgaaaaat ttgcaaaatt tggcaaaatt
     4981 cagaccgctt gtttaagcga tttggcaaac caacggttta atttaatcgt caatgccacc
     5041 tctctcggtc tgcaaggcaa atatgttgaa ttgcctgaat ctgttatagc atcggcgaat
     5101 atttacgata tgcaatatgc ccccaatatg caaactcctt ttctcaatta tgcaaggcgt
     5161 tgtggagcaa cagcgtgcca agacggatta ggaatgttgg ttggacaggc agcgtttgcg
     5221 ttttctttgt gggaagggca attccccgat gttgagcctg tactggctca attaaaatta
     5281 gaaatgcaaa cggtgaaata gcagaatata agctattatt ttataaacaa aaaatgctag
     5341 atttaagcca aaacgacgaa taactataca agcgaatcaa aaattcaaat tttttaaaaa
     5401 aagtatttga cggaaagccc taaaatcagc ataatgcact ccgttgaccg atatggtgaa
     5461 cgcaaatggc tacatagctc agctggttag agcacaacac tcataatgtt ggggtcgcaa
     5521 gttcgaatct cgctgtagcc accattgcgg gactggcgaa attggtagac gcaccagatt
     5581 taggttctgg cgccgcgagg tgtgtgggtt caagtccctc gtcccgcacc atttatcgat
     5641 taacaaaaat tagt
//
LOCUS       DANKBV010000139         5552 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.139, whole genome shotgun sequence.
ACCESSION   DANKBV010000139 DANKBV010000000
VERSION     DANKBV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.139"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..308
                     /locus_tag="PWY43_002110"
     CDS             <1..308
                     /locus_tag="PWY43_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2136961.1"
                     /translation="EQVEFYFKLPKRFKIPTPLGSYNPDWAVVFKGENKIYLVAETKN
                     TGKGIQAGVDEEKLDESERMKIDYARKHFKGLQRDYKDLDYRVVQKVEELSVEYCKI"
     gene            440..2872
                     /gene="gyrB"
                     /locus_tag="PWY43_002111"
     CDS             440..2872
                     /gene="gyrB"
                     /locus_tag="PWY43_002111"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL2136962.1"
                     /translation="MSEQTQATSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHM
                     VFEVVDNAIDEALAGHCKDIIVTIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTV
                     LHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLA
                     IIGNTEKTGTSVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIKLIDERDGKE
                     EHFKYEGGIKAYVEYLNEGKTHIHNTPFYLTTEKDGIGVEISLLWNDSYNENVYCFTN
                     NIPQRDGGTHLAGFRGALTRALKNYMDNSGVLKKADANIDASGDDAREGLVAVISVKV
                     PDPKFSSQTKDKLVSSEVRGAVESSMNEALSDYLAENPDDAKNIVTKIIDAARAREAA
                     RKAREMTRRKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAI
                     LPLKGKILNVEKARFDKMLSSQEVATLITALGTGIGRDDYNLEKLRYHKIIIMTDADV
                     DGAHIRTLLLTFFYRQMPELIENGYIYIAQPPLYKVKKGKQERYIQDNDEMSQYEIDI
                     ALEGAGLYVSEDAPALSGVALENLIAQYNGVQKLIERLSRRYPTLLLNELIYSKPLSV
                     EFAKNQANMTAWSEQFVATLMAKEVGGSFYRINTLFNEERQIYEPEIIVTTHGMDSTY
                     RLDYNFINGNEYARIVALGNELNDLLSDSAYVVRGERRQEISSFAEALDWLVKESRKG
                     LTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDF
                     IETNALYAQLDI"
     gene            complement(2925..3887)
                     /gene="cmoB"
                     /locus_tag="PWY43_002112"
     CDS             complement(2925..3887)
                     /gene="cmoB"
                     /locus_tag="PWY43_002112"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDL2136963.1"
                     /translation="MIDFRPFYQQIATTDLSAWLETLPLQLKQWEKTTHADYAKWAKV
                     ADFLPNSTACINLKDKVESISSKPLSEGEVKQITHHLKQLMPWRKGPYHLHGIHIDTE
                     WRSDFKWDRVLPHLAPLKDRTILDVGCGSGYHMWRMVGEGAKMVVGIDPTELFLCQFE
                     AVRKLLGNDRRANLIPLGIEQMQPLAAFDTVFSMGVLYHRKSPLDHLSQLKAQLVRGG
                     ELVLETLVIDGDINDVLMPADRYAKMKNIYFIPSVAALINWLEKVGFKNVRCVDEAIT
                     TLEEQRKTDWLENESLVDFLDPNDHSKTIEGYPAPKRAVILANA"
     gene            complement(3984..>5552)
                     /locus_tag="PWY43_002113"
     CDS             complement(3984..>5552)
                     /locus_tag="PWY43_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HDL2136964.1"
                     /translation="KKAALASALGEYFERLSTNYFWADFYLGQEIANSEFVHYPTEKW
                     FPIDDEAIPPMEMMDEFLWEYFDPNGEITPELLVDLQSGNYDRGIVTLPYVRQSDNET
                     VYIPQSIIGNLFVSNGMSAGNTKNEARVQGLSEVFERFVKNRIITEAISLPEIPQSVI
                     DGYPTIKASIEKLEQEDFPILCYDASLGGEFPVICVILLNQNNGTCFASFGAHPNFQV
                     AFERTVTELLQGRSLKDLDVFAPPSFNNEDVADHANLETHFIDSSGLISWDLFKRDAD
                     YPFVHWDFSGTTEQEFNNMMAIFNKHNQPVYIMDYEHLGVYACRILAPGMSNIYPSDD
                     LVYANNNMGMDWREILLDLPHFHHDAETYQELLDELDEQSIDDLTRVREFIGVVAEKG
                     SAMQTLRIGELKSMLHLALGNLEQASDWANWTANMNASVFSAERNNYYRCLIQSIELF
                     LDEERDSEQYRMVFEKMYGKQAVEAAWGAIQGGNPFHGLTASDESLENMTAHQKLLNA
                     YRKVQKAKVNPIAR"
BASE COUNT         1669 a         1121 c         1204 g         1558 t
ORIGIN      
        1 gtgagcaagt ggagttttat tttaaattac ccaaacgttt taaaattcca acgccattag
       61 gcagctataa tcccgattgg gcagttgtgt ttaagggaga aaataaaatt tacttggttg
      121 ccgaaacgaa aaatacaggc aaaggtattc aagcaggagt agatgaagaa aaattagatg
      181 aaagtgaacg aatgaaaatc gactatgccc gtaagcattt caaaggctta cagagagatt
      241 ataaggattt ggattatagg gttgtacaga aagtggagga gctgtctgtg gagtattgta
      301 aaatttaatc tgtggtcttg tagaattgag atagactgca atttcaacta aaatttaacc
      361 gcttgttttg cgatactttt acgcattttt ctgctataat cggcacaatt ttttcgattt
      421 caaattagta ggaataagaa tgtctgaaca aactcaagca acaagttatg attcttccag
      481 tattaaggtg ttacgcggct tagatgcggt gcgtaaacgc ccgggaatgt atatcggcga
      541 taccgatgac ggtacgggct tacaccatat ggtgtttgag gtggttgata atgcgattga
      601 cgaagcatta gccgggcatt gtaaagacat tattgtcaca attcattccg ataattcggt
      661 ttctgtgcaa gatgacggtc gtggaattcc ggtggggatt cacccggaag aaggcgtttc
      721 ggcagcagaa gtcattatga ctgttcttca cgcaggcggt aaatttgacg ataactccta
      781 taaggtttcc ggcggtttgc acggtgtggg cgtatcggtg gtgaatgcac tttcctctaa
      841 attacaactc actattcgcc gtgaaggaca cgttcacgag caattctata gcttaggtga
      901 gcctgatgca cctttagcga ttatcggcaa taccgaaaaa acggggacgt cggttcgttt
      961 ctggccaagt ttggatattt tcaaaaataa aaccgaattt gaatataaaa ttctgtctaa
     1021 acgcttgcgt gaactatcat ttttgaattc aggcgtttcg attaaattaa ttgatgaacg
     1081 agacggcaaa gaagagcatt ttaaatatga gggcggtatt aaagcctatg ttgagtattt
     1141 aaacgaaggc aaaactcata ttcacaatac gccattctat ttgaccactg agaaagacgg
     1201 cattggcgtg gaaatttccc tactgtggaa tgacagttac aacgaaaatg tgtactgctt
     1261 taccaataat attccacagc gtgatggcgg tacgcactta gccggtttcc gtggggcatt
     1321 aactcgtgcg ttgaaaaact atatggataa cagcggggta ttgaaaaaag ccgatgcgaa
     1381 cattgatgcc tcaggtgatg atgcccgtga aggtttagta gcggtgattt cggtgaaagt
     1441 gccggatccg aaattctcct cacaaaccaa agataaattg gtgtcgtcag aagtgcgtgg
     1501 agcagtggaa agctcaatga atgaggcttt atccgactat ttagccgaaa atccggacga
     1561 tgcaaaaaat atcgttacca aaattattga tgcagcccgt gcaagagaag cagctcgtaa
     1621 agcccgtgaa atgacccgcc gtaaaggggc gttagattta ggcggcttac cgggtaaatt
     1681 agctgattgt caggaaaaag atccggcgtt atcggagctt tacttggtgg agggtgactc
     1741 tgccggtggc tcggcaaaac aagggcgtga ccgtaaaaac caagcgattt tgccgttaaa
     1801 agggaaaatt ctgaacgtgg aaaaagcccg ctttgataaa atgctttctt cacaagaagt
     1861 agcaaccttg attaccgctt taggcacagg catcgggcgt gatgattata atttggaaaa
     1921 attacgttac cacaaaatca ttattatgac cgatgcggac gtggatggtg cacatattcg
     1981 tacgctttta ttaactttct tctaccgtca aatgccggag ctgattgaaa acggctatat
     2041 ttatattgca cagccaccgc tttataaagt gaaaaaaggt aagcaagagc gttatattca
     2101 agataacgat gaaatgtcgc aatatgaaat tgatattgca ttagaaggtg caggcttata
     2161 tgtgagtgaa gatgctccag cgttaagtgg cgtagcgtta gagaacttaa ttgcccaata
     2221 taacggcgta caaaaattaa ttgaacgttt aagccgtcgt tacccaacat tattacttaa
     2281 cgaattaatt tattcaaaac cgctttcggt ggaatttgca aaaaatcaag caaatatgac
     2341 cgcttggagc gagcaatttg tcgctacatt gatggcaaaa gaggtgggcg gcagtttcta
     2401 ccgtattaat accttgttca atgaagaacg ccaaatttat gagccggaaa ttattgtgac
     2461 tacacacggt atggacagta cctatcgttt agattacaac tttatcaacg gtaatgaata
     2521 cgctcgcatt gtggcattag gcaacgagct gaacgattta ctatctgatt cagcctatgt
     2581 ggtgcgtggt gaacgtcgcc aagaaatcag cagttttgct gaagccttag attggttggt
     2641 gaaagaatct cgtaaaggct taactattca acgttataaa gggttaggtg agatgaatcc
     2701 ggaacaatta tgggaaacca cgatggatcc ggtggctcgt aaaatgctac aggtcaatat
     2761 taccgatgcg attgcggcag ataaactctt cagtacgctg atgggcgatg aagttgaacc
     2821 acgccgagat ttcattgaaa ctaacgcttt gtatgctcag ttagatattt agttaaaaaa
     2881 tccgcatttg acatgcggat tttttattct cttaaactag cattttaagc attcgctaaa
     2941 atcaccgccc gcttcggagc agggtagcct tcaatggttt tgctatgatc gtttggatct
     3001 aaaaaatcca ctaaactttc attttccaac caatcggttt ttcgctgctc ttctagtgta
     3061 gtgattgcct catcaacaca acgcacattt ttaaagccga ctttttccag ccaattaatg
     3121 agtgcagcaa cagaaggaat aaaatagata tttttcattt tcgcatagcg atcagccggc
     3181 attaaaacat cattaatatc gccatcaatc accagcgttt ctaacaccaa ttcaccgcct
     3241 ctgactaatt gggctttgag ttgagaaaga tgatcaagcg gtgatttgcg atgataaagc
     3301 acccccattg aaaacacggt atcaaaagca gcaagcggtt gcatttgctc aataccaagt
     3361 ggaatcagat tagcccgtct atcattccct aagagtttcc gcactgcctc aaattggcac
     3421 aggaacagct cggtcgggtc aataccaact accatttttg ccccttcgcc taccattcgc
     3481 cacatatgat aaccgctacc gcagccgaca tctaaaatgg tgcggtcttt taacggtgca
     3541 agatgcggaa gcacacgatc ccatttaaaa tcggaacgcc attcggtatc aatatgaatc
     3601 ccgtgtagat gataagggcc tttacgccac ggcattagtt gctttaaatg gtgggtaatt
     3661 tgcttgactt ccccctcaga taaaggcttg cttgaaatag actcaacctt gtcttttaga
     3721 ttgatacaag cggtcgaatt tggtaaaaaa tctgcaactt ttgcccactt ggcatagtca
     3781 gcgtgggtgg tcttttccca ttgtttgagt tgtaagggta aggtttctaa ccaagcagaa
     3841 aggtcggttg tggcaatttg ttgataaaaa ggacgaaagt caatcatttt gaaatttatg
     3901 tctaggggag agacctatgc cctcccgaaa ggtagtaaat agaaaggaca ggcacagaac
     3961 ctacccctac gaaaaatata aaattatctc gcaatcggat ttactttcgc tttttgcact
     4021 ttgcgataag cgttcagtaa tttctgatgt gcagtcatat tttcaagcga ttcgtccgat
     4081 gcagttaaac cgtggaacgg gttaccgcct tggattgcac cccaagcagc ttcaaccgct
     4141 tgtttaccgt acattttctc aaacaccata cgatattgtt ccgaatcacg ctcttcatct
     4201 aagaacaact caatcgattg gattaagcag cggtaataat tgttacgttc tgctgagaat
     4261 acgcttgcat tcatattcgc tgtccagttt gcccagtctg aggcttgctc taagttacct
     4321 aaagctaaat gcagcatcga tttaagctca ccgatacgca atgtttgcat tgcagagcct
     4381 ttctctgcca ccacgccaat aaattcacgc acacgggtta aatcatcaat gctttgctca
     4441 tctaattcat caagcagttc ttggtaagtt tcagcatcgt gatggaaatg tggcaaatct
     4501 aacaagattt cacgccaatc catacccata ttgttattgg catagaccaa atcatcgctt
     4561 ggatagatat tcgacatacc cggtgcaaga atacggcaag cgtacacacc taaatgctca
     4621 taatccataa tatacacagg ctgattatgc ttgttgaaaa tcgccatcat attattaaat
     4681 tcttgctccg ttgtgccgga gaagtcccaa tgcacgaacg gataatccgc atcacgtttg
     4741 aaaagatccc acgaaattag accgcttgaa tcaataaagt gtgtctcaag gttcgcatga
     4801 tctgctacat cttcattatt aaatgaaggt ggggcaaaca catctaaatc ctttaagcta
     4861 cggccttgta ataactcggt tacggtacgt tcaaacgcca cttggaaatt cgggtgtgca
     4921 ccaaaagagg caaaacaagt accgttattt tggtttaaca agatcacaca aataactgga
     4981 aattctccgc ctaatgaagc gtcatagcat aaaatcggga agtcttcttg ctctagtttc
     5041 tcgatagagg ctttgattgt cggataaccg tcaatcacac tttgcggaat ttccggcaag
     5101 ctgattgcct cagtaataat acggtttttg acaaaacgct cgaacacttc ggataagccc
     5161 tgcactcgag cttcattttt tgtgttgcct gccgacatac cgtttgaaac aaacaggttg
     5221 ccgataatgc tttgcggaat gtaaaccgtt tcattgtcag attggcgaac gtaaggtagg
     5281 gtaacaattc cacgatcata atttcctgat tgtaaatcaa ccaataattc cggcgtgatt
     5341 tcaccattcg gatcaaaata ttcccataaa aactcgtcca tcatctccat tggtggaatg
     5401 gcttcatcat caatcggaaa ccatttttcg gttggataat gcacaaattc actattagca
     5461 atctcttgac ctaaataaaa atccgcccag aaatagttgg tagataaacg ctcaaaatac
     5521 tcacctaatg cagaggcaag agcggctttt tt
//
LOCUS       DANKBV010000140         5469 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.140, whole genome shotgun sequence.
ACCESSION   DANKBV010000140 DANKBV010000000
VERSION     DANKBV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.140"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..519)
                     /locus_tag="PWY43_002114"
     CDS             complement(<1..519)
                     /locus_tag="PWY43_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL2136965.1"
                     /translation="MNNLRIKMISPEIALAKYNELDVLEINHSKVKAKIALQGAQLLS
                     WQPKNTQQDVLWLSEIEPFQTGVAIRGGIPICHPWFGTDKKPAHGTARIQAWNLLEHT
                     SSADGIRVVLGLENQAKLEMILGEVCELYFTHLAPEPAQAALHTYFQVGDITKTEVQG
                     LATQCFDKLTNQE"
     gene            643..1647
                     /gene="fbp"
                     /locus_tag="PWY43_002115"
     CDS             643..1647
                     /gene="fbp"
                     /locus_tag="PWY43_002115"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDL2136966.1"
                     /translation="MKTLGEFIIEKQAEYPGAKGELSGILSSIRLAAKIIHREINRAG
                     LSQDILGTAGSENVQGEAQMKLDVFANETMKKALLAREDVAGFASEEDDNFVAFDNER
                     GRNAKYILMTDPLDGSSNIEVNVSVGTIFSIYRRVSPIGTPVTQEDFLQEGRKQVAAG
                     YVTYGSSTMLVYTTGNGVNGFTYDPSLGLFILSHPDLKTPTEGKYYSINEGQYVTFPQ
                     GVKRFIKYCQETDEAIKRPYSSRYIGSLVSDFHRNLLKGGIYIYPTSTVYPKGKLRLL
                     YECNPIAFLAEQAGGMATDGINPILDIKPTELHQRVPFFVGSTSMVKQAGEFMQEFAK
                     "
     gene            1831..3936
                     /locus_tag="PWY43_002116"
                     /pseudo
     CDS             1831..3936
                     /locus_tag="PWY43_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S6"
     gene            complement(4146..4874)
                     /locus_tag="PWY43_002117"
     CDS             complement(4146..4874)
                     /locus_tag="PWY43_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2136967.1"
                     /translation="MKSFDKVWFSAKELEELDLPDLPKQATNITRKALKELWIKRDAV
                     GVKGGGNEYHITSLPRKAQEALGFKSGINAIKDSIVREITPEEQNKGSELSGTVLNNI
                     ISMIDSFASIKVSAGFGSFNECVTKPDGQEPYADSLLHTLGVNAKKCAVFWASGDSMY
                     PTIDNGDQLLVDLSRNEIRGDNNIYIVQNGDSVWVKRVKLLWDGVELISDNREEYELI
                     KITADQAQNLQIIGQVVHIGHSLV"
     gene            5084..5311
                     /locus_tag="PWY43_002118"
     CDS             5084..5311
                     /locus_tag="PWY43_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2136968.1"
                     /translation="MNKKKRAQDMHRADIRAELMKKGITFSQLGIENGLSKTTVRNAL
                     DKPYKNGEEIIAKALGKTPAEIWPSRYPDVA"
     gene            5326..>5469
                     /locus_tag="PWY43_002119"
     CDS             5326..>5469
                     /locus_tag="PWY43_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDL2136969.1"
                     /translation="MKDWFSAKELAGISGLSKYPTNVTRQAKKEQWATRPLKGVKGGG
                     FEYH"
BASE COUNT         1731 a         1107 c         1065 g         1566 t
ORIGIN      
        1 ttcttgattt gtcaatttat caaaacattg tgttgctaag ccttgtactt ccgttttcgt
       61 aatatcaccg acctgaaaat aagtatgcaa tgcagcctgt gccggctccg gtgctaagtg
      121 agtaaaatac aactcacaga cttcgcctag tatcatctcg agttttgcct gattttctaa
      181 ccccaaaaca acacgaatcc catctgctga ggaagtatgt tccagcaaat tccacgcttg
      241 aatacgggct gtgccgtgag caggcttttt gtctgtacca aaccaagggt ggcaaatcgg
      301 aatgccgcca cgaatcgcca cacctgtttg gaaaggttca atttcactta accataatac
      361 atcttgctga gtatttttag gttgccaact cagtaactgc gcaccttgca atgcaatttt
      421 agccttcact ttggagtgat taatctctaa aacatccaat tcattatatt ttgctaatgc
      481 tatttcaggg ctgatcattt tgattcgtag attattcatc gtattgctcc tcatcttgaa
      541 agataaaatg tgaagtggat cacagaatta aagcttaaac cgctacctct ttaaaggtaa
      601 tcgttataat aagggcattc taacatagcg aaggagaaaa aaatgaaaac actcggcgaa
      661 tttattattg aaaaacaagc agaataccca ggggcaaaag gggaattaag tgggatttta
      721 tcttctattc gtttagccgc taaaattatt catcgagaaa tcaaccgtgc cggtttaagt
      781 caagatattt taggtactgc aggttctgaa aatgtacagg gtgaagccca aatgaaactt
      841 gatgtattcg ccaacgaaac gatgaaaaaa gcgttacttg cacgtgaaga tgttgccggc
      901 tttgcatcgg aagaagatga taattttgtt gcctttgata atgagcgtgg gagaaatgca
      961 aaatatattt taatgaccga tccgcttgat ggctcctcca atattgaagt gaatgtttct
     1021 gtgggaacaa tcttctctat ttaccgccgt gtctccccta tcggcacccc agtaacacaa
     1081 gaagatttct tgcaggaagg gcgtaaacaa gttgccgccg gttatgttac ttatggttct
     1141 tcaaccatgc tggtttacac cacaggcaat ggcgtgaacg gttttaccta tgatccgtca
     1201 ttaggcttat ttattctttc acaccctgat ctaaaaaccc ctaccgaagg aaaatattac
     1261 tccatcaatg aagggcagta tgttactttc ccacaaggtg tgaaacgctt tattaagtat
     1321 tgccaagaaa ccgatgaagc catcaaacgt ccatactctt cacgctatat cggctcgctg
     1381 gtgtccgatt tccaccgcaa tttattaaaa ggcggtattt atatttaccc aacctccacc
     1441 gtatatccaa aagggaaatt gcgtttactc tatgaatgca acccgattgc gtttcttgcc
     1501 gaacaagccg gaggaatggc gacagatggt attaatccga ttttagatat taagccaaca
     1561 gagctacacc agcgtgtacc gttctttgtc ggttcaacct caatggtcaa acaagcgggc
     1621 gaatttatgc aagaatttgc aaaataatgc tcctgcttat cactcaataa aactttcaac
     1681 aaagcagctg cgtattccgt agctgctttt gcctatctta agattgtaaa tttgtataaa
     1741 aaacaggtaa gacctcttgc caaataaata attatctata gaatacccca cctttttact
     1801 ccattaatat tagcaattta ttaacatttt atgataaata aaaactttcc actttcttac
     1861 cttgccagct ttgtggcatt tgctgttagt ttatctgcaa cagcctctgt tttcagagat
     1921 gatatcgatc ttcaatacta ccgtgatttt gcggagaata agggtaaatt caccgtaggt
     1981 gcaagcaata ttgaaattaa agataaaagt ggcaatacat tagatgcact cttcccaaat
     2041 gtaccgatgc cagattttag tgctgcaaac cgtaacttag ggatagcaac tctagttgca
     2101 ccgcaatatt tagtcagcgt tgcccataat acacaataca atactgtgga atttggcgcc
     2161 ccaggcacta atgccgatgc acaccactac acttacaaag tcgttgatcg taacgactat
     2221 ggaattgttg aaggtgggca acatcaagat tatcaagtgc cacgtctcaa taaattagtc
     2281 actgaggtcg cacctgcaac agtgactgat ctgggtaata atgcttcagc ctaccaagat
     2341 agtagtcgtt ttactcactt tgctcgctta ggctctggtc gtcaaatagt caaaaatacc
     2401 gagcataaag acaaccaaat ttcaacatct taccaatatt taaccggggg agttcattta
     2461 ccaatcgcgg tacataattc cgattactgg ctagatttta gaggaaatgc attaaacaat
     2521 agcccatatg gagcattaac tgcatttggt actcgtggtg atagcggttc aggtgtgtat
     2581 ggctacgatc agaaaacgaa acgctggcta ctactggcta cttacacctt tggtacgcct
     2641 gcaaacaact actacaaccg tgccggtatt atccgtcagg attatcacga taaacaattt
     2701 gccgaagata ttgccggcac attaaccaat gcgcaacaaa atgccgtttt tgaatggtct
     2761 gcacagggta aagacagtag cattggtaat aaagggaaaa acttaacggt ttcgcttgct
     2821 gatcgttcaa ttaaagacaa tagcgcttct ccattaggct atggtggcac acctcagcta
     2881 caacttccgc aagataacac cggaaaaacc ctaaacattg aaggacaaga tagcactatc
     2941 gtattaaaaa ccgatattga ccaaggtgct ggcgcattaa atttcaatgc taatgcaaca
     3001 gttcgccctg aaaatgatca aagctgggta ggagctggaa ttgtagtggc aaaaggtaaa
     3061 caagttaatt ggcaagtcaa aaacccacaa ggtgaccgtt tatctaaatt gggcaaaggg
     3121 acactacata ttaacggtaa aggcgaaaac cttggcgaca ttagtgttgg acaaggcact
     3181 gtcattttga accaacaagc gggtgaaaat ggcaaaaaat ctgcatttaa ccaagttggt
     3241 attgtgagcg gccgttcaac cgttgtatta aacagtgctg atcaagtcga tccaaataag
     3301 atttactttg gcttccgtgg tggtcgttta gatctcaacg gcaacaacat tgcgtttaat
     3361 cgtattcaaa atagtgatga tggtgcgcgt attgttaata atcacctaca aaaagctgca
     3421 acactcacca ttaatggccc gaagccccct gaagcaacgg acttaaaatg gggaacgtgg
     3481 aaagaaaaca gtgctgatat ttatgaatat atcaacccac atgctaataa ccgtaccgat
     3541 tactttacgc tcaaaggcaa tccaaaccaa tatatgccaa cgaatggcgc aagtaatgcc
     3601 cattggacct tcctgtcttc taacaaggat gcagcagtaa aacaagtact cgcgcaaaaa
     3661 ggtctggagc atcgctacaa tagctttaat ggttttattg gcgaaacaga taacacccag
     3721 cataacggtc gtttgaatgt ggtgtatgat ccaaaagcct ctacaccacc ggcaactgcc
     3781 tctgaagtaa cttggggtaa ggggctagta gctggtgcag atatttatct gttcaccaat
     3841 ccgaaaacga aaattcggga atactttgca ttaaaaggtg atccaaaaca acctgttcct
     3901 cgcggtggct taagcagcga gcattgggaa tttttagtgt ggggtactga tcttttttgc
     3961 atagagagaa agaaaaaaat ttgggcggaa aaataacggc gagccccgtg aatacaaggg
     4021 ttcgccgtta ttgtttggtt ggagctatgc aaaaaacaaa atgagcagga ttagaaaaaa
     4081 acgagcagtt tttgcgttat tttttgcata ggtttgatcg gttttaaaac tattttaaat
     4141 ctgttttaaa ctaaagaatg cccgatgtgt accacttgcc cgatgatttg taggttttgg
     4201 gcttggtctg ctgtaatctt tattaactcg tactcttctc ggttgtctga aataagctct
     4261 acaccgtccc atagcagttt tacacgctta acccaaacac tgtcgccgtt ttgtacaatg
     4321 taaatattat tgtcgcctcg tatctcatta cgacttaaat caactagtag ctggtcgcca
     4381 ttatcaatag tcggatacat cgagtctcca cttgcccaaa aaacagcaca cttcttggca
     4441 ttaacaccaa gcgtatgcaa caagctatca gcataaggct cttgtccatc aggttttgtt
     4501 acacattcat taaagctccc aaagcctgca gatactttaa tagatgcaaa actatcaatc
     4561 attgagatta tgttattcaa aacggtgcct gataattcag atcctttgtt ttgctcttca
     4621 ggtgtaatct ctcttactat gctatccttg attgcattta ttccactttt aaatccgagt
     4681 gcttcttggg ctttacgagg caatgaagtt atgtgatatt cattcccccc accttttact
     4741 ccaactgcat cacgttttat ccagagttct tttaatgcct ttcttgtaat gtttgttgcc
     4801 tgctttggaa ggtcaggcaa atccaattct tctagttctt tggctgaaaa ccaaacttta
     4861 tcaaatgatt tcataattaa ccccttctta atcaaaaatg attttaaaat caaaattgat
     4921 taagttattt aaaatcaata gcttacaaat agatttaaaa aagatttaaa aaatttatta
     4981 aatcactatt gatttagaaa agaataagta ttatatttag tcctgtagtt aatcaagtac
     5041 gctaaagcgt agcaaataaa ctaaatgtgg aggatctcac aaaatgaata aaaagaaaag
     5101 agctcaagat atgcatagag ctgatattcg agctgaattg atgaagaaag gaataacatt
     5161 ttctcagtta gggattgaga atgggctttc caaaacaaca gtaagaaatg ctcttgataa
     5221 accttataaa aacggtgaag aaattatcgc aaaagcatta ggaaaaacgc cagccgaaat
     5281 atggcctagt cgttaccctg atgttgcata acaaggatag ctgttatgaa agattggttt
     5341 agtgcaaaag aattagcagg tattagtggc ttatcaaaat acccaacaaa tgtaactaga
     5401 caagccaaaa aagaacaatg ggcaactcgt ccattgaaag gggttaaagg aggtggtttt
     5461 gaatatcac
//
LOCUS       DANKBV010000141         5380 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.141, whole genome shotgun sequence.
ACCESSION   DANKBV010000141 DANKBV010000000
VERSION     DANKBV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.141"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..627)
                     /locus_tag="PWY43_002120"
     CDS             complement(<1..627)
                     /locus_tag="PWY43_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL2136970.1"
                     /translation="MTTTYFHLMAKPSSFHCNIQCEYCFYLSKEQTIPPEKSQFMNDE
                     TLQNYIRHYIEANQSQRVDFVWQGGEPTLLGLDFYKQAVKYQQKFANGKQITNAIQTN
                     GIALNRQWASFFKENHFLVGLSIDGLSDIHDRYRISTNGKPTFERVKSAVDLLKEVGV
                     EFNTLTVVNDRNWQRGRETYQALKALGSRYMQFIPVVESHKGELQPFSV"
     gene            complement(717..2165)
                     /locus_tag="PWY43_002121"
     CDS             complement(717..2165)
                     /locus_tag="PWY43_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL2136971.1"
                     /translation="MNNIIYILLDQVRKDMLGTYGHQVVKTPNLDRLAKDGIRFNNAF
                     TPASVCGPARTSLFTGLMPSSHGIIKNGEKGGTGEIPQQNPNIAGVEGYNSFVIGKWH
                     VGTKSVPEDYGIAGHNFDGYGYPGSQVYKNLVFNQPPTHSNRYREWLAEKGYDAPEVS
                     RAYFGDNPHLRVQELCGLLSGSREQTIEWFIIDEAKKYIQASLDEKKPFFAWINFWGP
                     HTPCVVPEPYYSMYDPSQVVLDESFFKPLEGKPNHFRTISQMWGMWEASEDRWKEVIS
                     KFWGYITLIDDAIGELFRFLEENNLYENTFLVATADHGDAMGAHKMIEKGEFMFETTY
                     NIPLIIKDPHSTRVNEVDDNLVYLHDLTSTVYDVANQPIPTAFQGESILPITRQSQNN
                     DRKGLLCQLAGHFVYFEQRMWRRKDYKLVFNATDRCELYDVRKDPEEMHNLFYDEAYR
                     AVKKEMLEEMRQEMKRLNDPLENWVYRIIDEI"
     gene            complement(2257..3954)
                     /locus_tag="PWY43_002122"
     CDS             complement(2257..3954)
                     /locus_tag="PWY43_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL2136972.1"
                     /translation="MLLTIITFLVVTGLVGYISWQKTRNDDLSTSKGYFLAGRGLSAT
                     IIGCSMVLTSLSTEQLIGVNANSYAGNFSIIAWTVPAVIPLCFLALYLLPKYIRNGYT
                     TIPEFFENRFDRQTRLIMSGLFLVFYLFIVIPTALYTGAIAFNKIFNLETAFGLSYGA
                     AITYTVIAIGIVGAIYAIFGGLKAVAVSDTWNAVLLVIGALLVPVFALIYLGNGSMAE
                     GVKIITTTHVEKWNAVGSATDAVPWPTIFTGIMVVHFFYWTTNQAIVQRALGARDLKA
                     GQKGILIAALFLLLLPLILNLPGLLSFHILGEGLKPIDVSYPELVNKTFPTVLQGFFI
                     AALFGAILSTFNSFLNSAATIYCKDLLPSISKKQFTDAELIRYAKKVSAVMAVITMIF
                     GPLLMFGSDGIFLLTKRFAGFVNIPIVALFAVGLFNKTVSGKAARIALLAHVILYFCI
                     VWVFDVKINFVYVQAGLFVFDVALMLILGTFLKRATPYEDNKINHANVDLTGWEYAPL
                     AVASIVLGLFTLYAFLSPLGLASPDGNPAMVLAVYAVLQAVVCVVFFAKCKGKKVVN"
     gene            complement(4098..4649)
                     /locus_tag="PWY43_002123"
     CDS             complement(4098..4649)
                     /locus_tag="PWY43_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2136973.1"
                     /translation="MNNMPTPEQLMSCQLSVMSDLEKGVVIYSQGYHAREFYFLKSGL
                     IGLYHMLENGKESLVRVYKACDYFGFRTFFGNSTYHCSARVLQPAELIRITPLNFMEF
                     WANNSDLIISLLKQLSTELQDAEHRLSMVSYQKTQERVISSIHYLHQRYPNYHWTNRE
                     IAEFAGCETETAIRLRRELKKIS"
     gene            4748..>5380
                     /locus_tag="PWY43_002124"
     CDS             4748..>5380
                     /locus_tag="PWY43_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2136974.1"
                     /translation="MTMAVIIILIICGVVVNITSALFGIGGGVLMMPILHSLFPELPL
                     QVISACSLTIVIGSATINLCLFHHQQIKINRKNVLIWSLAMMLGVQIGFELSFLFSDN
                     SIIAIFILTLLTLSFKTLFHKPRVKPKPKEAVSNATMCSLGGLIAGITGIGGGSIMAP
                     IVTQIRSVSPKQVAVYTNYMMVIGGIGNLYGYLSRPFVLNSTLATWQIGYV"
BASE COUNT         1457 a         1223 c         1185 g         1515 t
ORIGIN      
        1 aacggaaaag ggttgtaatt cccccttgtg gctttccacc accggaatga actgcatata
       61 acggctaccg agggctttga gggcttgata ggtttctctg ccccgttgcc agttgcggtc
      121 gttcaccacc gtgagggtgt taaattccac gccaacctct ttcaataaat cgaccgcact
      181 tttcacccgt tcaaaggtcg gtttaccgtt ggtggaaatg cggtagcggt cgtgaatgtc
      241 gctcagtccg tcaatggata agcccaccag aaaatgattc tctttgaaaa agctcgccca
      301 ctgacggttt agggcaatgc cgttggtttg gattgcgttg gtgatctgct tgccgtttgc
      361 aaatttttgt tgatatttga ccgcttgttt gtagaaatct aagcccagca gggtcggctc
      421 gcccccctgc cacacaaaat ccacccgttg gctttggttg gcttcaatgt aatgacgaat
      481 gtagttttgc agcgtttcat cgttcataaa ctgcgatttt tctggcggaa tggtctgttc
      541 cttgctcaga taaaaacagt actcgcactg aatattacag tgaaaactgc tgggttttgc
      601 cattaggtgg aagtaggtgg tggtcattgt atctctaaat tgtaggggcg gactttatgt
      661 tcgcccattg tttacattaa ttttcgggcg ggtataaaac ccgcccctac aaacaattat
      721 atctcatcaa taatccgata cacccaattt tccagtgggt cgttgaggcg tttcatttcc
      781 tgtcgcattt cttccaacat ctcttttttc acggctcggt aggcttcgtc ataaaagagg
      841 ttgtgcatct cttcggggtc ttttctgaca tcgtagagtt cacaacggtc ggtggcgttg
      901 aagactaatt tgtaatcctt acgccgccac attcgctgtt caaaatagac aaaatgccct
      961 gccagttggc acaacaagcc tttacggtcg ttgttttggc tttgtctggt tatcggcaga
     1021 atactctcgc cttggaaagc ggtcgggatc ggttgatttg ctacatcata cacggtggag
     1081 gtgagatcgt gcaaataaac gaggttgtca tcgacttcgt tcacccttgt agagtgtgga
     1141 tctttgataa tcagcggaat gttgtaggtg gtttcaaaca taaactcgcc tttttcgatc
     1201 atcttatgcg cccccatcgc atcgccgtgg tcggcggtag caacgaggaa ggtattttca
     1261 tagagattgt tctcttccaa gaaacgaaac agctcgccaa tggcatcgtc aatcagcgtg
     1321 atgtagcccc agaatttaga aatcacctct ttccaacgat cttcgctcgc ctcccacatt
     1381 ccccacattt gtgaaatggt gcggaagtgg ttcggtttgc cctcaagcgg tttgaagaaa
     1441 ctctcgtcca ataccacttg gcttgggtca tacattgaat aatacggctc cggcactaca
     1501 cacggagtgt gcggccccca gaagttgatc cacgcaaaga acggtttttt ctcatcaagg
     1561 gaggcttgaa tgtatttttt cgcctcgtca atgataaacc actctattgt ctgctcgcga
     1621 ctgccggaaa gcaaaccaca gagttcctgc acacgcaggt gtggattgtc gccaaaatag
     1681 gctcgactga cttcaggtgc gtcatagcct ttttccgcca gccattcacg gtagcggttg
     1741 gagtgcgttg gcggctgatt gaacacgagg tttttataca cttggctacc cgggtagcca
     1801 tagccgtcaa agttatgacc ggcgatgcca taatcttcgg gaacggattt tgtaccaacg
     1861 tgccatttgc cgatcacaaa ggagttatag ccctccacgc cggcaatatt agggttttgt
     1921 tgtggaattt cgcctgtgcc gcctttttcg ccgtttttga taatcccgtg gctggacggc
     1981 attaagccgg taaacagcga ggtgcgagcc ggcccgcaga ccgaagccgg cgtaaaggcg
     2041 ttgttaaacc gaatgccgtc tttcgccaag cgatccaaat ttggggtttt caccacctga
     2101 tgcccgtaag tgccgagcat atctttacgc acttgatcta acaaaatata gatgatgtta
     2161 ttcataaatg acctctttgt tagggcaaga gccctgatga aatagtcttc caaatataca
     2221 ctcccctctt tagaaaagag gggagtgtat atttggttaa ttcacaacct ttttaccttt
     2281 acattttgca aaaaacacaa cacaaaccac cgcttgtagc accgcataca ccgccagcac
     2341 catcgctggg ttgccgtctg gcgaggctaa accgagcgga gaaaggaaag cgtatagcgt
     2401 gaataagccc aatacgatag aagccactgc cagtggggcg tattcccaac cggttaagtc
     2461 cacattagcg tggttgattt tgttgtcttc gtatggggtc gcacgtttta agaaagtgcc
     2521 taaaatcagc attaacgcca catcgaacac gaacaaaccg gcttgcacat acacgaagtt
     2581 gatttttaca tcaaacaccc aaacgatgca gaagtagaga ataacgtgtg cgagcaaggc
     2641 aatgcgtgcg gcttttcctg agacggtttt gttgaataac cccacagcga aaagtgccac
     2701 aatcggaatg ttcacaaaac cggcgaagcg tttggtcagc aagaaaatac cgtctgaacc
     2761 gaacatcagt aacggtccga agatcatcgt aatcaccgcc attaccgcag agactttttt
     2821 cgcatagcgg ataagttccg catctgtgaa ctgttttttg ctgatagacg gtagcaagtc
     2881 tttacagtaa atggttgccg ctgagtttaa gaacgagttg aaggtactca aaatcgcccc
     2941 gaacagtgcg gcaataaaga agccttgtag cacggttggg aaagttttat tcaccaattc
     3001 agggtaagac acatcaatcg gtttcaagcc ctcacctaaa atatggaagc tgagtaagcc
     3061 cggtaagttt aagataagcg gtagcaataa taagaacagt gccgcaatta agatcccttt
     3121 ttgaccggct tttaagtctc tcgcccccaa ggcacgttgc acaatggctt ggttggtcgt
     3181 ccagtagaag aaatgcacca ccataatacc ggtgaaaatg gtcggccacg gcactgcatc
     3241 ggtggcacta cccaccgcat tccatttttc aacgtgagta gtggtgatga ttttcacccc
     3301 ttctgccata ctgccattac ctaaatagat aagtgcaaac accggcacta ataatgcccc
     3361 aatgactaac aagaccgcat tccaagtgtc tgacaccgcc accgctttta agccaccgaa
     3421 gatcgcataa atcgccccaa caataccaat cgcaatcacc gtgtaggtaa tcgccgcccc
     3481 gtagcttaag ccgaaagcgg tttcaaggtt gaagatttta ttgaaggcaa tcgcaccggt
     3541 gtaaagtgcg gtcggaatga cgatgaaaag atagaacacc aaaaacaagc ccgacataat
     3601 caaacgggtt tggcggtcga aacggttttc aaagaactcc ggaatggtgg tgtaaccgtt
     3661 gcggatatat ttcggcaaca gataaagtgc caagaagcaa agcggaatga ccgccggcac
     3721 agtccacgca ataatcgaga aattaccggc ataagagttg gcattcacgc cgataagttg
     3781 ctcggtagaa agggaagtta agaccattga acagccgata atcgtagcac tcaagccacg
     3841 ccctgccaag aaataacctt tagatgttga aagatcgtca tttctggttt tttgccagga
     3901 tatatacccg actagtccag tcacaaccag aaaagtaatt attgttaata acatattctt
     3961 cacctcaata tgactatata aatgacatat cccattagag acggcaatag cataaatctt
     4021 atttttaacg atagaatatg atgtttgtca taatagatat gtgatggtga tcacaactat
     4081 gaataattta tgacaattta actgattttc tttaattccc gccgtaaccg aattgctgtt
     4141 tcggtttcac aacctgcaaa ttcagcgatt tcacggttag tccagtgata attggggtaa
     4201 cgttgatgta aatagtggat gctgcttatt acccgctctt gcgttttttg ataggacacc
     4261 atcgataaac gatgttctgc atcttgtaat tctgtagaga gctgttttaa tagggagata
     4321 attaagtcag aattgtttgc ccaaaactcc ataaaattta atggggtaat tcgtatcagt
     4381 tctgccggct gcaaaactcg ggctgaacaa tggtaagtgc tgttaccaaa aaaggtccga
     4441 aaaccaaagt aatcacaggc tttataaacc cttactaagc tctctttacc attttccagc
     4501 atatgataca gccctatcaa gccactcttt aaaaaataaa attctctggc gtgataaccc
     4561 tgagaataaa tgacgactcc tttttctaga tcagacatta cacttaattg gcaagacatc
     4621 aattgttcgg gggttggcat attgttcatt gattatcctt attattttat ttttgccata
     4681 tatgattgcg atcataatcc tttatttaga aaattttgca tacttatcgc actttaggag
     4741 atggattatg acaatggccg tgataattat tttgatcatt tgtggtgttg tagtcaatat
     4801 cacttctgca ctatttggga ttggcggcgg tgttttaatg atgccgattt tacattcact
     4861 gtttcctgaa ctgcctctcc aagtgatttc cgcctgctct cttaccattg tgattggttc
     4921 agccactatc aatttatgct tattccacca tcaacagata aaaatcaatc gaaaaaatgt
     4981 gttgatttgg tcacttgcga tgatgttagg cgtacaaatt gggtttgaac tcagttttct
     5041 tttctctgat aacagcatta tcgctatctt tattctaacc ctgctgacac tttcatttaa
     5101 aaccctattt cacaaaccaa gagttaaacc taaaccaaaa gaagcagtat ctaatgccac
     5161 aatgtgcagt ttaggcggtt taattgcagg tattaccggc attggcggcg gttcgattat
     5221 ggctccgatt gtcacgcaaa tacgctcggt ttcaccgaaa caggttgcag tttatactaa
     5281 ctatatgatg gtgattggcg gaatcggcaa tttatatggc tacttatccc gcccttttgt
     5341 gttgaattca accttagcaa cttggcaaat cggctatgtg
//
LOCUS       DANKBV010000142         5313 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.142, whole genome shotgun sequence.
ACCESSION   DANKBV010000142 DANKBV010000000
VERSION     DANKBV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.142"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            9..1118
                     /gene="rnd"
                     /locus_tag="PWY43_002125"
     CDS             9..1118
                     /gene="rnd"
                     /locus_tag="PWY43_002125"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDL2136975.1"
                     /translation="MIHYQWVETNEQLEAVCEQARKTKVVALDTEFIRTRTYYPILGL
                     IQLFDGQQVSLIDPNTISDFSPFVSLLADKNIIKVLHACSEDLEVFEYQFKQLPEPML
                     DTQVMAAFAGIGISMGFAKLVSHYLNIELDKGASRTDWLARPLSAEQLQYAAADVWYL
                     LPVYEKLAQALTKSTWQSAVAEECVALLKKAKQPINKEKAYKNIANAWRLNREELAIL
                     QVLAKWRIEEAQKRNLALNFVVKEASLVQIAKSCPKHTSSLLEFMHPNEVRIHGKKIL
                     WLVEQGKAIESNDYPELIQRLVDEKGYKYYMQAMLQKLLEIQPLDIVPELVASKRQLN
                     QLFKWYIDGKPSEKIPELLIGWRKPFGEQLLSVLS"
     gene            1175..1657
                     /gene="lrp"
                     /locus_tag="PWY43_002126"
     CDS             1175..1657
                     /gene="lrp"
                     /locus_tag="PWY43_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDL2136976.1"
                     /translation="MENKKPPKALDAIDLKILNELQRNGKISNIELSKRVGLSPTPCL
                     ERVKRLEKNNVIMGYKALLNPELLDAPLLVIVEITLVRGKPDVFEEFNKAIQQLDEIQ
                     ECHLVSGDFDYLLKTRVADMASYRKLLGTTLLRLPGVNDTRTYVVMEEVKQTNYLLLK
                     "
     gene            1668..4328
                     /locus_tag="PWY43_002127"
     CDS             1668..4328
                     /locus_tag="PWY43_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HDL2136977.1"
                     /translation="MIEQLKPHLKGKENLIKLMLLLTGFLGVYLLIAWASYSPLDNAW
                     SVASSITQTTLNKTGHFGAWSIDMLYAMFGKVAVLVPIALIIFSIYSLGMGLSVDMKW
                     KTVFIRLASFLLLMVGLAGLFSVLFSNSAYYLSGGFVGGVWETILSDTIGQFGALLIA
                     MLFTIAGLYFCSVQSLLPILTQFYEWIMAKSEESKTAESLQHLQNTEQKQPLVTENEA
                     VEVVDDHKPQFTDISAFKRPNIGGLRHSENVEIDNREMYEIERDLELPTINILIQPDM
                     VNQVAEQATSGYELPNEESNVLQMPTIRLNTAIENEVDTIENSSSLEDLKIAESMLPQ
                     TQLDNDIEDTEEAIDDVREITPEFMVTPPKAMKDVSEQPAYPKGYGDTLIHPLLQRKV
                     ITEKPTTPLPTLDLLDKAPIQSQQITEQEIRETSVRLEAELANFGVKATVEDVLVGPV
                     VTRYEIQPAAGVKASKITNLASDIARGLMFKAIRITEVIPNKPYMGIETPNKHRETVW
                     LRDVLDSDEFRNTTATLPMALGKDISGEPVVVDMAKMPHLLVAGQTGGGKSVGVNTMI
                     LSLLFKLTPEQVRFIMIDPKVVELSIYNDIPHLLTPVVTDMKKAANALRWAVEEMERR
                     YLLVSHLQVRNIEGYNAKIDQAAAMNLPIPDPTWRPGDSMDSLPPPLQKLSYIVLIVD
                     EFADLMMSAGKEVEEYIMRIAQKARAVGIHLILATQRPSTDVITGVIKANIPSRIAFT
                     VASQIDSRTILDSGGAEALLGRGDMLYSGAGSPDIIRIHGAFMKDEEVQRVADNWRAR
                     RKPNYLESIVESRSEEADGKNDGGTGDLDPLFDEVVEYITETGSVSISNIQRRFSLGF
                     NRAARIVDQMEAQGIVSEPLKGGKREVLAR"
     gene            4480..>5313
                     /locus_tag="PWY43_002128"
     CDS             4480..>5313
                     /locus_tag="PWY43_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDL2136978.1"
                     /translation="MRLDDIKESKNVEDRRFNNVGRTKVGRGKGGILTFIIVLVGAYY
                     GVDLTGLMGGGVEYEEQTSALNESEEKQLRTLSAKVLTTTENIWTSYFQQNGLTYKEP
                     SLVLYRGAIQTACGTGQSAMGPFYCPVDQKVYLDLSFYDDMRKKLRASGDFAFSYVIA
                     HEVGHHVQNLLGITSKTQRAQMKASSKAEANQISVNVELQADCFAGVWGHQLAKANRL
                     EEGDVEEAFNAAQAVGDDRLQQQSRGYVVPDSFTHGSSAQRLAWFRKGLTTGNPAVCN
                     TF"
BASE COUNT         1670 a          963 c         1205 g         1475 t
ORIGIN      
        1 tggagtcttt gattcactac caatgggttg aaacaaatga gcaattagag gccgtatgtg
       61 aacaggccag aaaaacaaaa gtagttgcct tagatacaga gtttatccgt acacgtacct
      121 attatccgat attgggttta attcaactct ttgatggcca acaggttagc cttattgatc
      181 caaatacgat ttctgacttc tcgccttttg tgtcattatt agccgataaa aatatcataa
      241 aagtattaca tgcctgtagt gaggatttag aagtttttga gtatcaattt aagcaattgc
      301 cagagccgat gttggatact caggtgatgg cagcctttgc cggcattggt atttcgatgg
      361 ggtttgcaaa attggtttcc cactacttaa atattgaact agacaaaggc gcttcccgaa
      421 ccgattggct tgccagaccg ctaagcgctg aacaattaca atatgcggca gctgatgttt
      481 ggtatttatt gccagtttat gagaaattgg cacaagcatt aacaaaaagc acttggcaaa
      541 gtgctgtagc agaggagtgt gtcgctttgt tgaagaaagc gaaacagcct atcaataagg
      601 aaaaagccta taaaaatatt gccaatgctt ggcgattgaa tcgggaagag ttagccatat
      661 tacaagtatt agcgaaatgg cggattgaag aagcacaaaa gcgcaatctt gccttaaatt
      721 ttgtcgtgaa ggaggcaagt ttagttcaaa tcgcaaaaag ctgtcctaaa cacacttctt
      781 cgctgttaga atttatgcac ccaaatgaag tcagaattca cggaaaaaaa atactatggt
      841 tggttgagca aggaaaagca atagaatcga atgattatcc tgaactcatt cagcgcttgg
      901 tggatgaaaa gggttataaa tattatatgc aagcgatgtt gcaaaaatta ttagaaattc
      961 aaccgcttga tatcgtaccg gaattggttg caagtaaacg tcaattaaat cagctattta
     1021 aatggtatat tgatggaaaa ccatcagaaa aaattcctga attattaatc ggttggcgaa
     1081 aaccttttgg tgaacagctg ctatcggttt tatcttaaat ttcattcggg ttacatagac
     1141 tatgtacagt ataattgcct aaaaaggagt ttatatggaa aataaaaaac caccaaaagc
     1201 attagatgcg attgatttaa aaatcttaaa cgagctacaa cgtaacggga aaatttctaa
     1261 tattgaatta tcaaagcgag ttgggctttc tccaacgccc tgcttagaac gggtaaaacg
     1321 gctagaaaaa aataacgtaa ttatgggcta taaagcttta cttaaccctg aattacttga
     1381 tgccccttta ctggtgattg ttgaaattac tttggtgcgt ggtaagcctg atgtatttga
     1441 agaatttaat aaagcgattc aacaacttga tgaaattcaa gaatgccacc tggtatcggg
     1501 ggattttgat tatctgttaa aaacccgtgt ggcggatatg gcatcgtacc gtaagttatt
     1561 aggcacaaca ttgcttcgtt tgcccggcgt aaatgacact cgaacctatg tggtgatgga
     1621 agaagtaaaa caaaccaact atttgttgtt aaagtaagga gaattgggtg attgaacaac
     1681 ttaaaccaca cctaaaaggc aaagagaact tgatcaaact tatgcttttg ttgaccggct
     1741 ttttgggcgt ttatttgcta attgcttggg caagttatag tccattagat aatgcttggt
     1801 ctgttgcaag cagcattaca caaacaacct tgaataaaac aggtcacttt ggtgcttgga
     1861 gtattgatat gctctatgca atgtttggta aggttgcagt tttagtacct attgcattaa
     1921 ttattttctc aatttatagc ttgggaatgg gtttaagtgt cgatatgaaa tggaaaactg
     1981 tcttcattcg cctagccagt ttcttattat tgatggtcgg tcttgccggg ttatttagcg
     2041 tgcttttttc aaactcagct tattatctgt cgggtggctt tgtcggtgga gtttgggaga
     2101 caatattatc tgatactatc ggacaatttg gtgccttatt gattgcaatg ctttttacta
     2161 tagctggctt atatttttgc tctgttcaat cattattgcc aatattaacg caattttatg
     2221 agtggataat ggcaaaatca gaagagagca aaacagctga atctcttcag catttgcaaa
     2281 atactgagca aaaacaaccg cttgtaactg aaaatgaagc ggtggaagtt gtagatgacc
     2341 ataaaccgca atttaccgat atctccgcct ttaaacgccc aaacattggt ggtttaagac
     2401 actcggaaaa tgtagagata gataaccgag aaatgtatga aattgaaaga gatcttgagc
     2461 taccgactat taatatttta atacagcctg atatggttaa ccaagttgcg gaacaagcta
     2521 catcgggcta tgagttacca aacgaagaat ccaatgtatt gcaaatgccg accattcgct
     2581 taaataccgc tatcgaaaac gaggtggata caatcgaaaa cagttcatca ttggaagacc
     2641 tgaaaatagc agaatctatg ttacctcaaa ctcagcttga taatgatatt gaagatactg
     2701 aagaagctat tgacgatgtg cgtgaaatta caccggaatt tatggtaacg ccgcccaagg
     2761 cgatgaaaga tgtgtctgag cagccagcct atccaaaagg ctatggtgat acgttaattc
     2821 acccgttgtt acaacgaaaa gtgataaccg aaaaaccaac aacaccattg ccaacgctag
     2881 atttgttgga taaagcaccg attcaaagcc agcaaattac cgaacaggaa atacgagaaa
     2941 cctctgtgcg gctagaagca gaattagcaa actttggcgt aaaagctacc gttgaagatg
     3001 tattggtcgg tccggtagtg acccgttatg aaatccaacc ggctgccggg gtaaaagcct
     3061 ccaaaattac caatttagcg agcgatatag ctcgtggctt aatgtttaaa gcgattagga
     3121 ttactgaagt tatcccaaac aaaccttata tgggtattga aactccgaat aaacaccgtg
     3181 aaacagtgtg gttgcgtgat gtgctagaca gcgatgaatt ccgcaatacc actgcaacat
     3241 tgccaatggc actaggtaaa gacatcagtg gtgagcctgt ggtggtcgat atggcgaaaa
     3301 tgccacactt actggtggca gggcagaccg gtgggggtaa atcggttggg gtgaatacga
     3361 tgattttaag tctattgttc aaacttacac cagaacaagt ccgctttatt atgattgacc
     3421 ctaaagtggt ggagctttcc atttataacg atattcctca cttattaacg ccggtagtaa
     3481 ccgatatgaa aaaagctgcc aacgcactac gctgggcggt ggaggaaatg gaacgccgtt
     3541 atttgctggt aagccattta caggtgcgta atattgaagg ctataatgcg aaaatcgacc
     3601 aagcggcagc gatgaatttg ccgattccag atccaacgtg gcgaccgggc gattcaatgg
     3661 atagccttcc gcctccgcta caaaaattaa gctatattgt gctgattgtc gatgagtttg
     3721 cggatttaat gatgtctgcc ggtaaagaag tggaagagta cattatgcga attgctcaaa
     3781 aagcccgagc ggtgggaatt cacttaattc ttgctaccca acgtccgtca accgatgtga
     3841 ttaccggcgt gattaaagcc aatattccaa gccggattgc gtttacagta gcgagccaaa
     3901 ttgactcaag aactatttta gattcaggtg gagcagaggc attattaggg cgaggcgata
     3961 tgctctattc aggagctggc agcccggata ttattcggat acacggtgcg tttatgaaag
     4021 acgaagaagt gcaacgcgtg gcggataatt ggcgggcaag aagaaaacca aactacttgg
     4081 aaagcattgt ggaatcccgc tcggaagagg ctgatggcaa aaacgatggc ggaacgggcg
     4141 atttagatcc gctatttgat gaggttgttg aatatatcac ggaaaccggt tcggtctcta
     4201 tcagtaatat tcaacgccgc ttctcgcttg gttttaaccg tgcggctaga attgtggatc
     4261 aaatggaagc tcaaggcatt gtttctgagc cacttaaagg tggcaaacga gaagtgctgg
     4321 cgaggtaaaa cacatacaag cggttagatt tctgtgaaat tttgcaaatt ttgacaaaaa
     4381 tctaaccgct tgtatttatc taatgctaaa taagcatagt ataaggctag tctttgggct
     4441 aaaattaacg caatttttac atcttttaag gttgaatata tgcgattaga tgatattaaa
     4501 gaaagtaaaa atgttgaaga cagaagattc aacaatgtgg gtagaactaa ggttggtaga
     4561 gggaaaggtg gaatattaac ctttattatc gtgcttgttg gagcttatta tggtgttgac
     4621 ttaacaggtt taatgggcgg tggcgtagaa tatgaggagc agacatcagc attaaatgaa
     4681 agtgaagaaa aacagctaag aaccttatcc gctaaagtat taacgaccac agaaaatatt
     4741 tggacttcct acttccagca aaatggtcta acttataaag agccgtcttt ggtgctttac
     4801 cgtggggcaa ttcaaactgc ctgtggcacc ggtcaatctg caatgggtcc tttctattgc
     4861 cctgtcgatc aaaaggttta tttagattta tcattctatg atgatatgcg taaaaaatta
     4921 agagcatcgg gcgattttgc cttctcttac gtgattgcac acgaggttgg ccaccacgtc
     4981 caaaatttat tgggcataac aagcaaaacc caacgggctc aaatgaaagc ctcatctaaa
     5041 gccgaagcaa accaaatttc agtcaatgta gaattgcaag ctgactgttt tgccggcgtt
     5101 tggggacacc aactcgcaaa agccaaccga ttagaggaag gcgatgtgga agaagccttt
     5161 aatgctgccc aagcagttgg agatgaccga ttacagcaac aaagtcgtgg ctatgtcgtg
     5221 ccggatagct tcacccacgg ctcatctgca cagcgtttgg cttggtttag aaaaggttta
     5281 accacaggaa atcctgcagt ttgtaataca ttt
//
LOCUS       DANKBV010000143         5311 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.143, whole genome shotgun sequence.
ACCESSION   DANKBV010000143 DANKBV010000000
VERSION     DANKBV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.143"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..699
                     /locus_tag="PWY43_002129"
     CDS             <1..699
                     /locus_tag="PWY43_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDL2136979.1"
                     /translation="EVNIQKSRYFQQKSLFNELKQELIQLKSTQLSLENEIETRKTEF
                     NNQIIRYEMQKSDLNIRLMEFESVSDIIVNASLDGKIESTSVTVGQIIKENDPLAQIL
                     PQNKGNYQLVMWVPNSAISFIKQGDEVNIRYEAFPFEKFGQFKGKITSISTLPASLQE
                     LSFYKNLPVNLEQGIPLYKILIELADQNVKYNDTSLYFMSGMKAEATLFLEKRKLYEW
                     IFFPMYQLRKNMEQ"
     gene            699..1490
                     /locus_tag="PWY43_002130"
     CDS             699..1490
                     /locus_tag="PWY43_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HDL2136980.1"
                     /translation="MTGIKQLKFDFRRRIPVILQTETAECGLACLAMMLGYYDKNTNL
                     FELRSQYGTSSRGVTLHTLISIANDCGLITRPLSLELDEVPQLRLPCILHWDFNHFVV
                     LTHASEKQFIIHDPAFGKRKLSRAEFSNHFTGVALEVWSEVKFEKSNNENTISLYETL
                     KHISGIKGALVKIFALSMLIELIALLMPIGTQLVMDHVMQAKDQSLLLIICIGLFLFT
                     LFRTMVSMFRAWVSLKMNYLIDFQWTASFFSHLLKLPLDFFENAK"
     gene            1868..2785
                     /locus_tag="PWY43_002131"
                     /pseudo
     CDS             1868..2785
                     /locus_tag="PWY43_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2787..3455)
                     /locus_tag="PWY43_002132"
     CDS             complement(2787..3455)
                     /locus_tag="PWY43_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL2136981.1"
                     /translation="MKYLYALIFSLFFSSVYAAERQLQCKVVGISDGDTLTCLVNKTP
                     LKIRLQHIDAPEQAQPYGNKAKQALAQFVFQQNITAIVSGYDRYQRLLATLYLNDKNI
                     NLALVENGMAWAYTTKREYIEAQDQAQQAKIGLWQDKFPINPSQWRKTHTSTLPQAVK
                     KPENIANQPPTIDCQIKLSCNQIGNYAQAKRYFQQCGWKELDGNNDSIPCNKLYRQEQ
                     KNRN"
     gene            complement(3499..4866)
                     /locus_tag="PWY43_002133"
     CDS             complement(3499..4866)
                     /locus_tag="PWY43_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDL2136982.1"
                     /translation="MSIDTILNQINSFVWGPPLLILISGVGLYLTFSLKGIQLSQLGQ
                     AFRYMFKPEKGKGQSGDISAFAALSTALAATIGTGNIVGVATAIHSGGPGALFWMWLI
                     ALFGMATKYAEGLLAIKYRTKDRAGFVAGGPMYYIELGMGKKWRWLAKAFAFFGVLVA
                     LLGIGTFPQVNGITHALESTFDVPIIFSAIILTLIVTAIILGGVQRISKIATIVVPFM
                     AIGYVIAALSVILLNMEKVPQAIITIVHAAFNPQAALGGAVGFTVMQAIQLGVARGIF
                     SNESGLGSAPIAAAAAQTKEPVRQGLISMTGTFLDTIIVCTMTGLVIVISGAWAGDVK
                     GAELTTTAFTQGLNSSFGAIIVTVGLVFFAFTTILGWCYYGERCFVYLTRGRTQYIKL
                     YRLLFIALIASAPFLQLDTIWTLADIVNGLMAFPNLIALIALRKVIISETKDFFARLK
                     TGKAE"
     regulatory      complement(4985..5080)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT         1605 a          965 c         1069 g         1672 t
ORIGIN      
        1 gaagtgaata ttcagaaaag ccgttatttc caacaaaaat cactgtttaa tgagctaaag
       61 caagaattaa ttcaattaaa atccacccaa cttagcttgg aaaatgaaat tgaaacccgt
      121 aaaaccgaat ttaataatca gattattcgc tatgaaatgc aaaaaagcga tttaaacatt
      181 cgtttaatgg aatttgaatc tgtttcagat attattgtta atgcctcttt agacggaaaa
      241 attgaaagta ccagcgtaac agttgggcaa attattaaag aaaatgaccc gcttgcacaa
      301 attctgccac agaataaagg aaattatcag ctagtgatgt gggtaccgaa tagtgccatt
      361 tcatttatta aacaaggcga tgaggtgaat attcgctatg aggcttttcc gtttgaaaaa
      421 ttcgggcagt ttaagggcaa aattacgtca atttcaacct taccagcttc tttgcaagaa
      481 cttagttttt ataaaaatct ccctgtaaat ttagagcaag gtattccgct ttataaaatt
      541 ctgattgagc ttgccgatca aaacgtaaaa tataatgata cctcccttta ttttatgagt
      601 ggaatgaaag ccgaagccac gttattttta gaaaaacgta aactgtatga gtggatattt
      661 ttccctatgt atcaattacg caaaaatatg gagcaataat gacaggaatt aagcagttaa
      721 aatttgattt tcggagaaga atacctgtta ttttgcaaac tgaaacagct gaatgtggct
      781 tagcctgttt agcgatgatg ttgggctatt acgataaaaa caccaattta tttgaattgc
      841 gaagtcaata tggtacttcc tctcgtggcg taacgctgca tactttaatt tctattgcaa
      901 atgactgtgg tttaataact cgccctcttt ctctagagtt agatgaagta ccacaacttc
      961 gtctgccttg tattcttcat tgggatttta atcattttgt agtattaact cacgcaagtg
     1021 aaaaacagtt tattatccac gatcctgctt tcggtaaacg taaattatcc cgtgctgaat
     1081 tttccaacca ttttaccgga gtcgctttag aagtttggtc tgaagttaaa tttgagaaaa
     1141 gcaacaatga aaataccatt agtttgtatg aaaccttaaa gcatatttct ggcataaaag
     1201 gtgcattagt gaaaattttt gccctttcaa tgctcattga actcattgcc ttattgatgc
     1261 caattggaac acagttagtg atggatcacg ttatgcaagc gaaagatcaa tcattacttt
     1321 taattatctg tattgggcta tttttattta ccctttttcg cacaatggtc agtatgttta
     1381 gggcttgggt ttccttgaaa atgaattatt taattgattt tcaatggaca gccagtttct
     1441 tttctcatct cttaaaatta ccgcttgatt tctttgaaaa cgccaagtag gtgatattca
     1501 ttcccgattt agttcattaa aaaccattca gaaaactcta acttcaagca ttattaccac
     1561 gattattgat acgattatga tcatcagttt aattattatg atggtgttat atggcggttg
     1621 gctcttttgg gtagtattag ctttttcctt tatctatttt attcttcgaa tggttactta
     1681 tttagtttat cgtcaactga atgaagagca gattattaaa ggagcaaaag cccattcgca
     1741 ttttatggaa actttatatg gtattacaac gttaaaatca ttaggattgg aaaataaacg
     1801 acaagaacag tggatgtcgc tcaatgcgga tgcttttaac acatcaatta aggtaactaa
     1861 atttgatatg ctgttttccg gtattcatac ctttatttct accatcgaac agattttaat
     1921 tctttggatt ggggcgacaa tggtgattga aaatgtgatg acattaggaa tgtttgtcgc
     1981 ttttaatgcc tatcgaggct ctttctcttc acgaatgggg aatttaattg atattatttt
     2041 tagcctaaaa attctttcat tacatagaga gcggattgca gacattgctt taaatgaagt
     2101 cgaagatgaa agtaaatatc aagaactcag atttgaacat aaccaaacag atatagaagt
     2161 taaagacctt tgtttcaaat acgatccttt ttctaaaaat gtgcttgaaa atgtcaattt
     2221 agtgatcaga agtggggaaa gtgttgctat taccgcacca tcaggttatg gcaaaacaac
     2281 gttattaaaa ttaattgcag gtttattaaa accaacctca ggttctgtgt attttaacgg
     2341 cattgatatt tatcagttag gattaagtca atatcggagc caaattgctt gcgtattaca
     2401 ggaagataaa ttcttttctg gttccatttt agagaatatt gttagttttg aaagtaatta
     2461 tgatcgagaa tgggctatcg aatgtagcaa acttgcgaat attcacaatg atattatggc
     2521 aatgccgatg aattatgaaa ccttattagg agaattagga aataatttgt ctggcggaca
     2581 aagataacga ttgtttattg ctcgagcttt atataaaaag cctaaaattc ttttgatgga
     2641 tgaggcgact agccatttag atgaagaaaa tgagaagctt gtcaatcaag ctatttcacg
     2701 aatgaagata acaaggatta ttattgctca tagacaatcg actattcagt cggcggatag
     2761 aattattgcc ctgaatgaca ggtaaattaa ttcctatttt tctgttcttg ccgataaagt
     2821 ttattgcacg gaatgctgtc gttgttgccg tctagctctt tccagccgca ttgttgaaag
     2881 tagcgttttg cctgtgcgta attgccgatt tggttgcagc ttaatttaat ttggcaatca
     2941 atcgttggtg gctggtttgc aatattttcc ggttttttga ccgcttgtgg taaggttgag
     3001 gtgtgtgttt ttcgccattg gctcgggtta atcgggaatt tgtcctgcca taagccgatt
     3061 ttagcttgct gtgcttgatc ttgtgcttcg atatattccc gctttgtggt gtaagcccac
     3121 gccataccgt tttccactaa ggctaagttg atgtttttat cgtttaagta gagggtggca
     3181 agtaatcgtt ggtaacgatc atagcctgaa actatggcag taatgttttg ttgaaacacg
     3241 aattgagcca gagcttgttt ggctttgttg ccgtaaggct gcgcttgttc gggggcatca
     3301 atatgttgta gccttatttt tagtggtgtt ttattgacca aacaagtgag ggtgtcgcca
     3361 tcgctaatgc caacgacttt gcattgtagt tggcgttcgg cggcatatac cgatgagaaa
     3421 aataagctaa aaatcagggc gtaaagatat ttcattatta aaaaagagag ggctaataag
     3481 ccctcattct atcatttttt attcggcttt acctgtttta aggcgagcga agaaatcttt
     3541 tgtttcacta ataatcactt tgcgtaacgc aatcaatgcg attaaattcg ggaatgccat
     3601 taagccgttc acgatgtctg ccagcgtcca aattgtatcg agttgtaaaa atggagcaga
     3661 ggcgatgagt gcaataaaca gcaagcggta taatttaatg tattgggtgc gacctcgagt
     3721 gagatagaca aaacagcgtt caccgtagta gcaccagcct aaaatggtgg taaaggcaaa
     3781 gaagactaaa ccaactgtca caattatcgc accgaatgag ctgtttaaac cttgagtaaa
     3841 agcggttgtg gtgagttctg cgccttttac atcgcctgcc caagcccctg aaatcacaat
     3901 tactaagccg gtcattgtgc agacgataat agtgtctaag aacgtgccgg tcattgaaat
     3961 taggccttga cgtaccggct ctttggtttg ggcggcagcg gcggcgatgg gggcagagcc
     4021 taatcctgat tcattcgaga aaataccacg tgcaacccct aattgaatcg cttgcatgac
     4081 ggtgaatccg actgctccgc ctaatgccgc ttgtgggttg aaagctgcgt gtacaatagt
     4141 aataattgct tgtgggactt tttccatatt cagtagaatg acggataaag cggcgatgac
     4201 atacccaata gccataaacg gtacaacaat ggttgcaatt ttagaaatcc gctgcacgcc
     4261 acctaaaata atagcggtaa cgataagggt taaaatgatc gcactgaaga taatcggcac
     4321 atcaaaggtg ctttctaagg cgtgggtaat gccattcact tgggggaacg tgccaatacc
     4381 aagcaaagcg accaatacgc caaaaaacgc aaaggcttta gcaagccaac gccatttttt
     4441 tcccattccc agttcgatat agtacatcgg accaccagcc acaaatccgg ctctatcttt
     4501 agttcgatat ttaattgcca ataagccttc ggcatatttc gtagccatac cgaataaggc
     4561 aatcagccac atccaaaata atgctccagg cccgcccgaa tgaatagcgg ttgccacgcc
     4621 gacaatattc cccgtgccga tagtagctgc tagggcagtg gaaagggctg caaaagcgga
     4681 aatatcgccc gattgccctt tgcctttttc cggtttgaac atatagcgaa aggcttgccc
     4741 tagttggctt aattgaatac ctttgagtga aaaggttaaa tataacccta cgcctgaaat
     4801 taaaatcagg agtggcgggc cccaaacaaa actgttgatt tgattaagaa tagtgtcgat
     4861 agacatttga tgttcctcgt aaaaactgct tacaaggaaa gaaaaggaat gcaagagaca
     4921 agcggtcaaa aaacggaaat tttttgcaaa gaattagcaa aaaaacaccg cttgtatcgt
     4981 ataacttttc tcccctgtcc ttttgcctga gcgtttcaca cattgctgta ttttcgcctt
     5041 cggcggcgga ttttaatccg ctctctccaa gggttcgtcc agtaacagtc cccgcagtgt
     5101 tcactgcacc tgaaagagtt tacctcgtcg gtgaaatgtt aattcatttc tctccagcta
     5161 ccttcatccg aacaatctaa tttattttat aatgtaaaat aaaaaagatt tgccaaattc
     5221 tagcaactct tttgcttccg tcaagtgaat ttaacgatat ttttgttctt tcggtacttc
     5281 ttctaaatcg ctaaataata cggcatattt t
//
LOCUS       DANKBV010000144         5310 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.144, whole genome shotgun sequence.
ACCESSION   DANKBV010000144 DANKBV010000000
VERSION     DANKBV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.144"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(230..1870)
                     /gene="pgi"
                     /locus_tag="PWY43_002134"
     CDS             complement(230..1870)
                     /gene="pgi"
                     /locus_tag="PWY43_002134"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL2136983.1"
                     /translation="MQKINPVQTFAWNALEQHKADNLSIPQLFAEDPNRFDKYSLRFE
                     DQLLVDFSKNAINGKTLTLLRQLANECGLESAKNAMFSGKKINRTENRAVLHVALRNR
                     ANTPIAVDGKDVMPEVNAVLAKMKDFCERVISGEWKGYTGKTITDVINIGIGGSDLGP
                     YMVTEALRPYKNHLNMHFVSNVDGTHIAEVLRKVNPETTLVLVASKTFTTQETMTNAL
                     SARKWLLDSAKDESAVAKHFVALSTNAKEVEKFGIDTANMFEFWDWVGGRYSLWSAIG
                     LSIALSLGFENFEQLLAGAHAMDKHFLITPIEKNIPTTLALVGIWNNNFLGAESEAIL
                     PYDQYMHRFAAYFQQGNMESNGKFVGRDGKPVTHQTGPIIWGEPGTNGQHAFYQLIHQ
                     GTKLIPCDFIAPAQTHNPLSDHHNKLLSNFFAQTEALAFGKSKETVEKEFLDAGKTLE
                     EVAEIVPFKVFSGNKPTNSILVQKITPYSLGALIAMYEHKIFVQGVLFNIYSFDQWGV
                     ELGKQLANRILPELETDEKINSHDSSTNGLINQFKAWR"
     gene            complement(1903..2151)
                     /locus_tag="PWY43_002135"
     CDS             complement(1903..2151)
                     /locus_tag="PWY43_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HDL2136984.1"
                     /translation="MLTPKNLEAIAQQLHNALPQSLKNVGNDLDEKFKQILQAQLARL
                     DVVTREEFDVQSQVLLRTREKLTELEKRLDELTQRTDE"
     regulatory      2276..2320
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2324..2992
                     /gene="queC"
                     /locus_tag="PWY43_002136"
     CDS             2324..2992
                     /gene="queC"
                     /locus_tag="PWY43_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDL2136985.1"
                     /translation="MTHQKPKAVVIFSGGQDSTTCLFQAIAEFGKENVEVVTFQYGQR
                     HSIELEKARWISNDLGVKQTLIDTSVIKAITTNALMDNNAEIQQQGSTPNTFVDGRNA
                     LFLLYTAIYAKGQGIQTIFTGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYNFNIRTP
                     LMYLTKKETWALADKLGAFDYIRDHTHTCYLGVEGGCHDCPSCVLREKGLNEYLAERE
                     VSNV"
     gene            2985..3407
                     /gene="queD"
                     /locus_tag="PWY43_002137"
     CDS             2985..3407
                     /gene="queD"
                     /locus_tag="PWY43_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDL2136986.1"
                     /translation="MFKIAKEFSFDMAHMLDGHDGKCQNLHGHTYKLQVEVAGELFQE
                     GAKRGMVMDYSDLKAVVKQHILEPMDHAFIYDLQSERESQVAQLLIDLNSKVYGIPTR
                     TTAEEMAKYMFEKLEKVGLPVSLIRLWETPTSYCEYSK"
     gene            3407..4033
                     /locus_tag="PWY43_002138"
     CDS             3407..4033
                     /locus_tag="PWY43_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDL2136987.1"
                     /translation="MAELISNPRFPIVEIFESLQGEGFNTGMPAIFVRFGKCNLTCPW
                     CDTNYNQFEQWTLSDILAKVRSYSAKNVIITGGEPTIQPNLSLLLEQFKKEGYFLAIE
                     TNGLKEVPAQIDYIATSPKRMYQEKYQRRCIEFANEVRIVVDGEVQGFCEQIESQIKA
                     EHYYLSPCEVEGKMNLLETITQIGLLNQRPNKPKWQLSIQTHKIVGIE"
     gene            complement(4129..5196)
                     /locus_tag="PWY43_002139"
     CDS             complement(4129..5196)
                     /locus_tag="PWY43_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136988.1"
                     /translation="MKFNKKLILTFAATLVLSACGGSSSGGSPSTPNQPKPVLVPKTQ
                     NTPLAQNTPQVQNAPQVQNVPQVQNVPQMENNTQSQNNSQIQDQPEVAPPATQIAPSI
                     PEKKIDGSFDKIGTVILDAEKQTITLNKFILVDKPDSPKKYDRVSGKPIIKEKNQIIL
                     PLADISTGALTSDQNADYLIGRSGDLFYGVYHDTQSNILVEEADKFSKYFVIYDENRV
                     NSKVSQDLTATYDKKGGVVYGANAGIKEFEARLNKLGDVNIEFKNGKANGNISDENSV
                     KPIFTITGDTKSLVISPTADNPVISVILNERKQSYSQGMEKAVMDIKFIDSAKGAGDQ
                     KYLIGEAKSESWQAIMVSEKQ"
BASE COUNT         1567 a          960 c         1077 g         1706 t
ORIGIN      
        1 tgagtgaaga gtagaggtaa aatagtaagc ctttttaacc agtttattta ttgtacctat
       61 tttagaaaaa agaaaagtag aaaagagata ttaggtaaac tcatagtgca aaagaatata
      121 gaaaaaactg cttgtgtaga aatggtgctt agttcagtcc tcagctaaaa ttttagaaaa
      181 aaagcataga gattttttac ctctatgctt tttattgtat tgactatatt taacgccaag
      241 ccttgaattg gttaattaaa ccatttgtgg agctgtcgtg gctgttgatt ttttcatcgg
      301 tttcgagttc gggtaaaatg cggtttgcaa gctgtttgcc taactccacg ccccattggt
      361 cgaaactgta aatgttgaac agcacgcctt gcacgaagat tttgtgttca tacattgcaa
      421 ttaatgcccc taagctgtac ggggtgattt tttgcaccaa gatagagttg gtcggtttgt
      481 ttccgctgaa cactttaaac ggcacgattt cagctacttc ttccaacgtt ttacctgcat
      541 ctaaaaactc tttttcaact gtttcttttg atttaccgaa agctagtgct tcagtttgag
      601 caaagaagtt agaaagcagt ttgttgtgat gatcgcttaa cggattgtga gtttgtgccg
      661 gtgcgataaa atcacaagga attaattttg tgccttgatg aatgagttga tagaatgcgt
      721 gctgaccgtt agttcccggt tcgccccaaa taatcggacc tgtttggtga gtaaccggtt
      781 tgccgtcacg ccctacaaat ttaccgtttg attccatatt gccttgttgg aaataagccg
      841 caaagcggtg catatattgg tcgtaaggta aaatcgcttc tgattcagca cctaagaaat
      901 tgttattcca aatgccgact aaagctaacg tagttggaat gtttttctca atcggcgtga
      961 ttaagaaatg cttatccatt gcgtgagcac cagcaagtag ctgctcgaag ttctcaaaac
     1021 ctaatgaaag ggcaatcgaa agcccgatgg cagaccataa tgagtaacga ccgccaaccc
     1081 aatcccagaa ttcaaacata ttagcggtat caataccgaa tttttccact tctttggcat
     1141 tggttgaaag tgccacgaaa tgttttgcta ccgcagattc atctttggca gaatcaagta
     1201 gccatttgcg agctgaaagg gcattggtca tcgtttcttg agtggtaaag gtttttgaag
     1261 ccactaacac taacgtggtt tcaggattaa ctttacgcaa cacttcagca atatgcgtgc
     1321 cgtccacgtt agacacaaag tgcatattaa ggtgattttt ataagggcgt aatgcctctg
     1381 ttaccatata aggacctaga tccgagccac caataccgat attgatcaca tcagtaatcg
     1441 ttttgccggt atagcctttc cattcgccgg aaataacacg ctcgcagaaa tctttcattt
     1501 tcgctaacac ggcattcact tccggcatta catctttgcc atcgactgcg attggtgtat
     1561 tggcacggtt acgcagtgca acgtgcaata cggcacggtt ttcagtgcgg ttgatttttt
     1621 tgccggagaa catcgcattt tttgctgatt ccaaaccaca ttcatttgcc agttggcgaa
     1681 gtaaggttaa ggtttttccg ttgatcgcat tttttgaaaa atcgactaat aattgatctt
     1741 caaaacggag ggaatatttg tcgaaacggt ttggatcttc ggcaaaaagt tgaggaatag
     1801 aaaggttgtc tgctttatgt tgctctaagg cattccaagc aaaagtttgc acagggttaa
     1861 ttttttgcat ctgagtttcc ttatactaaa ttaaaaaatc gattattcat ctgttctttg
     1921 agtcagttca tcaagacgtt tttctaattc ggttaatttt tcacgagtgc ggagtaacac
     1981 ttgagattga acatcaaatt cttcacgagt gacaacatca agacgggcta attgggcttg
     2041 aaggatttgc ttaaattttt catctagatc attaccgaca ttttttaagc tttgcggtaa
     2101 ggcgttatgg agttgttgag caatcgcttc taagttcttc ggcgtgagca tatttaaatt
     2161 ccttactata aatgagtatc tcaattttat tgaattttgg gtttaagaga aagcaaattc
     2221 tttgtaaaat atcacctttc gcaaaataca agcaaaaaaa gaccgcttgt attttttatt
     2281 tgtggttcgt aaacctccca cacaaaaaaa ctaaggacac aaaatgactc atcaaaaacc
     2341 aaaagccgtt gtgatttttt ccggcggtca agactctacc acttgtttat tccaagctat
     2401 tgctgaattt ggcaaagaaa atgtggaagt cgttaccttt caatatgggc aacgccattc
     2461 gattgaactt gaaaaagcac gctggatttc taacgattta ggcgtcaaac aaacgctgat
     2521 tgatacttcg gtgattaaag cgattactac caatgcgtta atggataata atgctgaaat
     2581 tcaacaacaa ggcagtacac ccaatacctt tgtggatggg cgtaatgcct tatttctgct
     2641 ttataccgcc atttatgcca aagggcaggg cattcaaact atttttaccg gcgtgtgtga
     2701 gaccgatttt agtggttacc ctgattgccg agatgtattt gtaaaatcaa tgaatgtaac
     2761 gctaaattta gcgatggact ataatttcaa cattcgcacg ccattgatgt atttaactaa
     2821 aaaagaaact tgggcattag cagataagct gggtgctttt gattatattc gagaccatac
     2881 ccacacctgt tatttaggcg ttgaaggtgg ttgccacgac tgcccgagtt gcgtattgcg
     2941 agaaaaaggc ttgaatgaat atttagcaga aagggaggta agcaatgttt aaaattgcta
     3001 aagaatttag ttttgatatg gctcatatgc tagatggaca cgatggcaaa tgccaaaact
     3061 tacacggaca cacctacaaa ttgcaagttg aggtggcagg tgagctattt caagagggag
     3121 ctaaaagagg aatggtaatg gactattcgg atctaaaagc agtggtaaaa caacatattt
     3181 tagagccgat ggatcacgct tttatttacg acttacagag tgagcgtgaa agccaagtag
     3241 cacaattatt aattgattta aactccaagg tttatggtat tccaactcgt accacagccg
     3301 aagaaatggc gaaatatatg tttgaaaaat tagagaaagt gggcttaccg gttagcctaa
     3361 ttcgcttgtg ggaaacgcct acttcttatt gtgagtacag caaataatgg cggagttaat
     3421 ttcaaaccct cgttttccga ttgttgagat ctttgaaagc ttacaagggg aaggctttaa
     3481 taccggaatg ccggctattt ttgtacgttt tggcaaatgc aacctcactt gcccgtggtg
     3541 cgataccaat tacaatcaat ttgagcaatg gacgttaagc gatattcttg ccaaagtaag
     3601 aagctattct gcgaaaaatg tgattattac cggaggcgag ccaacgattc agcctaattt
     3661 atcgttatta ttagaacaat ttaaaaaaga gggatatttt ttagcgatcg aaaccaacgg
     3721 cttaaaagag gttccggcac aaattgatta tattgcgacc agcccaaagc gaatgtacca
     3781 agaaaagtat caacgccgtt gcattgaatt tgcgaatgaa gtccgtatcg tcgttgatgg
     3841 agaagtacag ggtttctgtg agcaaataga aagccaaatt aaagctgagc attactatct
     3901 ttcaccttgt gaggtagagg gaaaaatgaa cttgctggaa accatcaccc agattgggtt
     3961 attaaatcag cgtccaaata agcctaaatg gcagctaagt atccaaacgc ataaaatcgt
     4021 ggggattgaa taagtagaag ttagaagatg attttctaca atttaaaaga ctgagccgat
     4081 attatcggac tcagtctttt ttacttcagt ttttagcaga agataacttt attgtttctc
     4141 gctaaccatt atagcttgcc agctctcgct ttttgcttca ccgattaagt atttttggtc
     4201 gccagctcct tttgcagaat caataaattt aatatccatt acagcttttt ccataccttg
     4261 actgtaactc tgtttacgtt catttaatat tacagatata acagggttat ctgctgttgg
     4321 agaaattact aaactttttg tatcaccagt gattgtaaag atcggtttta cggaattttc
     4381 atcacttata ttaccgtttg ccttaccatt tttgaattca atgttaacat ctcccagttt
     4441 atttaaacgt gcttcaaact cttttatacc tgcatttgca ccatatacaa cccctccttt
     4501 tttgtcataa gtggctgtta agtcttgaga tactttactg ttaacacgat tttcatcata
     4561 aataacaaaa tatttgctga atttatccgc ttcttctaca agaatattac tttgtgtatc
     4621 gtgataaacg ccatagaaca gatctccgct acgtcctatt agataatcag cgttttgatc
     4681 agatgttaat gctccggtag aaatatctgc caacggtagt atgatctggt tcttttcttt
     4741 aataattggt ttgccactaa cacgatcata ttttttgggg ctatcaggtt tatcaactag
     4801 aataaattta ttgagtgtaa tagtttgttt ttctgcatca agtattactg tcccaatttt
     4861 atcaaaagat ccatctattt tcttttcagg tattgacgga gcaatttgtg ttgctggagg
     4921 agcaacttct ggctgatcct gtatttggga attattttga ctttgggtgt tattttccat
     4981 ctgaggaaca ttttgtacct gaggaacatt ttgtacctga ggagcatttt gtacctgagg
     5041 agtattttgt gccagaggag tattttgtgt ttttggtact agtactggtt tgggttggtt
     5101 cggtgttgaa ggcgaacctc cgctactact tcctccgcaa gcacttaaaa ctaaggttgc
     5161 ggcaaatgtt aaaattaatt ttttattgaa tttcattggg ttattcctta aatataaaaa
     5221 cgagactatg agaagtctat catatttttt tatatgaaat aagaatgaat ggtctgagga
     5281 gttttacgta aacgtttccc ttatgaaaaa
//
LOCUS       DANKBV010000145         5280 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.145, whole genome shotgun sequence.
ACCESSION   DANKBV010000145 DANKBV010000000
VERSION     DANKBV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.145"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..694)
                     /locus_tag="PWY43_002140"
     CDS             complement(<1..694)
                     /locus_tag="PWY43_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL2136989.1"
                     /translation="MEKRTLALLKVHGTAVLFGASVIFGALIQSSAETIVLGRVMIAF
                     AIISLYFLWKKQPLVKLNLQQMAQLAFSAALLAAHWVTFFVAVKVGGVAVATLGFASF
                     PAFVALFESLFFREKLRFREVVLLIAITVGLILVTPEFTFGNQATQGLLWGVLSALVY
                     GILAVANRRTMSKISGVQASWWQYIFATLFLLPLSITEFPSVSTQDWFWIFCIGFLCT
                     TLAYTLFISSLDT"
     gene            complement(694..1179)
                     /locus_tag="PWY43_002141"
     CDS             complement(694..1179)
                     /locus_tag="PWY43_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HDL2136990.1"
                     /translation="MPILAILFGIFFYIYCEISLLVNIGSIIGVLPTILLLVVISFLG
                     LWIVKLRGVYTLFSIKQDLSQGKIPTEAVGNSVMFIVAGILLIIPGFLSDILAILCVL
                     PFSRKWIQAFVINSVKNKVFMHFTAQTSSFSATSAQDSNTFDAEFERKPDEQDQHNRI
                     K"
     gene            complement(1181..2504)
                     /gene="pepQ"
                     /locus_tag="PWY43_002142"
                     /pseudo
     CDS             complement(1181..2504)
                     /gene="pepQ"
                     /locus_tag="PWY43_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
     gene            2515..2757
                     /gene="tusA"
                     /locus_tag="PWY43_002143"
     CDS             2515..2757
                     /gene="tusA"
                     /locus_tag="PWY43_002143"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDL2136991.1"
                     /translation="MTQLTVNKELDTLGLRCPEPVMLTRKTIRNMEEGEILHIIADDP
                     ATTRDIPSFCEFMDHQLIQKEVDSTPYHYWIKKGLE"
     gene            complement(2824..3255)
                     /gene="rraB"
                     /locus_tag="PWY43_002144"
     CDS             complement(2824..3255)
                     /gene="rraB"
                     /locus_tag="PWY43_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDL2136992.1"
                     /translation="MYNLDELRTETDETISSLLADGSDPEALYIIEHHVAHHDFDKLE
                     KLVVDAYKLGYEISEAEEVEDENGKVIFVCDIVSEIHLNADLITAQQKELLPLIEKAG
                     AEYEGWGTYFEDPNADEDEYGDDGEFFDDENDDFEDEPRRH"
     gene            complement(3270..3881)
                     /locus_tag="PWY43_002145"
     CDS             complement(3270..3881)
                     /locus_tag="PWY43_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL2136993.1"
                     /translation="MKLNYHKTHFLTSAPDIRHLPEDTGVEIAFAGRSNAGKSTALNA
                     LTNQKSLARTSKTPGRTQLINLFEVETNCKLVDLPGYGYAAVPEQMKIQWQKALGEYL
                     QKRECLGGVVILMDIRHPLKDLDQQMIEWAVSANLPVLLLLTKADKLSQSARSKQVKM
                     VREAILPFQGDIQVEAYSALNKIGIDKLSNKLDSWFAPKLITE"
     gene            complement(3883..4299)
                     /locus_tag="PWY43_002146"
     CDS             complement(3883..4299)
                     /locus_tag="PWY43_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL2136994.1"
                     /translation="MSWYKALFSFNGRLNRRGFWIGFGIHFLFLFMVAMWINLAELSL
                     LSVLPLIITGFSLSAIIIKRLHDRNRSAKALLMALVPIICYLAAPYSQGIMQILLGQV
                     MPAFIGMLLFLEWGVFAGNPEPNQYGEQGLSFKFRE"
     gene            complement(4303..5133)
                     /locus_tag="PWY43_002147"
     CDS             complement(4303..5133)
                     /locus_tag="PWY43_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL2136995.1"
                     /translation="MFEYFLSLSPIWQAFLAGLFTWGCTIFGSSFVYFFKSVNRKLLD
                     SMMGFAAGVMIAASFWSLLAPSLEYAEADYGSLAWLPAAIGFLAGGAFIRLIDYVVPH
                     LHLSKPIEQAEGNDQLKQGLSKSTLLFLAITIHNIPEGLAIGVAFGALATQVSGVDAS
                     IMGAIGLAIGIGLQNIPEGSSLALPIRGEGHSRKKAFWYGSMSAVVEPIAAVIGAVFV
                     LSMTAILPYALAFAAGAMIFVVVEELIPESQSSGNTDIATLSLMLGFVVMMVLDVTLG
                     "
BASE COUNT         1688 a         1196 c          984 g         1412 t
ORIGIN      
        1 tggtatcaag gcttgaaata aacagcgtat aggcaagggt tgtacataaa aaaccgatac
       61 agaaaatcca aaaccaatct tgggtggaaa cgcttggaaa ttcagtgatg gagagaggca
      121 acagaaaaag tgtagcaaaa atatactgcc accagcttgc ttgcacaccg gaaattttgc
      181 tcatggtacg gcgattcgct accgccaaaa taccatacac caaagcagat aacacgcccc
      241 acaataaacc ttgagtggct tgattaccaa aagtaaattc aggggtaact aaaattaaac
      301 caaccgtgat ggcaattaat agcaccactt cacgaaagcg taatttttca cggaagaaca
      361 ggctttcaaa taacgccaca aatgccggaa agctggcaaa gcctaaggtt gctaccgcca
      421 caccacctac tttaaccgcc acaaaaaaag taacccagtg ggcagctaat aaagctgcag
      481 aaaatgcaag ttgagccatt tgctgtaagt tgagctttac aagcggttgt tttttccata
      541 aaaaatacaa agagatgata gcaaaagcaa tcatcactcg cccaagcaca atggtttcag
      601 ccgagctttg aattaatgct ccgaagatca ccgaggcacc aaataaaacc gctgtgccgt
      661 gtactttaag tagtgcaagt gttcttttct ccactatttg atcctattat gttgatcttg
      721 ctcgtccggc ttgcgttcaa attctgcatc aaacgtgttg ctatcttgcg cagatgtagc
      781 agaaaaagac gaagtttggg cggtaaaatg cataaacacc ttattcttta cgctattaat
      841 tacgaaggct tgaatccatt ttcggctaaa gggtaacacg cataaaatcg ctaaaatatc
      901 acttaaaaag ccgggaataa tcagcaaaat gccggctaca ataaacatca ccgagttgcc
      961 gactgcttca gtcggtatct tgccttggct taaatcttgc ttgatggaaa agagggtata
     1021 aacgcccctt aacttcacaa tccataaccc taaaaatgaa atcacgacca gtaacaaaat
     1081 cgtgggtaac acgccaatga tcgagccaat attcaccagc agcgaaattt cgcaatagat
     1141 ataaaagaaa ataccaaaca gaatcgctaa aataggcata ttatcctccc aacgttgcgg
     1201 cagcgtttgc cgtcagattt tctgccccat tggctcgcat gacaatattg tcttcggtac
     1261 gaatcccgcc aaaggctttg aaagcctcaa ttttctgcca gttaaaggca gaagataagg
     1321 cactgttttt ccaaggctta agtagcatct caataaaata aaaacccggc tcaatggtaa
     1381 gcaccatatt ttcctgtagc tcacgggtac aacgcaaact tgggtaaacc tctggaggag
     1441 ctttatggct gcctcgggca ttttgctgaa aaccgcccac atcgtgcact tgaataccca
     1501 ataaatgccc tagtccgtgt gggaagaacg tacggctaat cccttgttca aaaatttgct
     1561 cggctgaaag atgaataaat tgatgttccg ctaataattg cgaaatcatc tgctgcattc
     1621 gggtgtgata atgcaggtag ttcaccccaa tactcagctc agcaataatc tgcaacttca
     1681 actgttccat tttccgccac cagctcggca aattcagaac cgctgtcaaa ggcataggtg
     1741 cgggtgagat cggaggcata accatttacc gttgtgccgg catcaattaa aaaactgtgg
     1801 cgttttgcgt gggtgtcaaa atctaacgcc gtataatgta gcaccgcacc attttgatta
     1861 atcgccacaa tattgccata aggaacattg agatcagatt gctgcgtcgc tttcaaatat
     1921 tcaaggttga tttcaaattc gcttttgccg acaaaaaatg cctcttttgc tacttgatgc
     1981 ccttttaagg ctgaaaattg ggcttggtaa atactttcaa tttcaaactc cgttttgatg
     2041 gagcgggcaa aattcaggct attcagcact ttctgcggat taatatggct aaagcctaac
     2101 tttttggcta gctcaacatc ttcaccgata aaggcacaag cggtcggatt ttggataaag
     2161 gttgcaattt cattctctgc tctcaacaaa acccactcaa attccgcagc gaaaaaggcc
     2221 tccgttggca cttttagagt ggaaacccaa taatcttcag gggcataaaa atagattttc
     2281 ggcttatttt tgccatctaa aaataaccag caattttcag cttggggata tgggaaaaag
     2341 taattaaaat aagggttaat tttaaacggg acggtttgat cgtctaaaaa taaggttttt
     2401 gccttgccgg cataaatcca taaaccgtct aaaaaggcat tttccagcaa gctttgcact
     2461 gtctgttgca aacgtgcgag atgcgtagag aatagatttt tcataaggaa taaaatgact
     2521 caattaactg taaataaaga attagatacc ttggggctac gttgcccaga gccggtgatg
     2581 ctcacccgta aaacaatccg taatatggaa gagggagaaa ttttgcatat catcgccgat
     2641 gacccggcca ctacccgtga tattccgagt ttctgcgaat ttatggatca ccaattaatt
     2701 caaaaagagg tggactccac cccataccat tactggatta aaaaaggctt ggaataaacc
     2761 acaagcggtc gattttagcc ataaaattgc aaatttatgc acaaaatcga ccgcttgttt
     2821 taattaatga cggcgaggtt cgtcctcaaa atcgtcattt tcatcatcga aaaattcacc
     2881 gtcatcgccg tactcatctt catcagcatt cggatcttca aagtaggttc cccagccctc
     2941 atactccgca ccggcttttt caattaatgg cagtaactct ttttgttgtg cggtaatgag
     3001 atccgcattc aagtgaattt cgctcacaat gtcgcataca aaaatcactt taccattttc
     3061 gtcttccact tcttccgctt cggaaatttc atagcccagc ttataggcat caactactaa
     3121 tttttccaat ttatcaaaat cgtggtgagc aacgtggtgt tcaataatat aaagggcttc
     3181 agggtctgaa ccatcggcta ataatgatga aatggtttca tcggtttctg tgcgtaattc
     3241 atcgagattg tacatattga attccttact tattctgtga ttaattttgg tgcaaaccag
     3301 ctatctaatt tgttcgacag cttatcaata ccgattttat tcagtgcgga ataagcctct
     3361 acttggatat caccttggaa tggtaaaatc gcttctctta ccatttttac ctgcttagag
     3421 cgtgccgatt ggcttaactt atccgctttg gtcaacagca gtaacaccgg caagttggct
     3481 gacactgccc attcaatcat ctgttgatct aaatctttca acgggtggcg aatatccatt
     3541 aaaatcacca caccgcctaa acattcacgc ttttgtaaat actcgcctaa tgctttttgc
     3601 cactggattt tcatctgttc aggtactgct gcatagccat agccaggtaa atccactaat
     3661 ttacaattcg tttcaacctc aaacaagtta atcaactgcg ttcgccccgg tgttttggaa
     3721 gttcgggcta aacttttttg gttagttaaa gcgttcaaag cagtcgattt accggcattt
     3781 gaacgcccgg caaaagcaat ctccacacct gtatcttcag gtaaatgacg aatatccggt
     3841 gctgaagtta aaaaatgggt tttatgatag tttaatttca ttttattctc taaatttaaa
     3901 agataagcct tgctcgccat attgattcgg ttcaggatta ccggcaaata caccccattc
     3961 taaaaagagc aacattccaa taaatgccgg catcacctgt ccaagcaata tctgcataat
     4021 tccttgtgaa taaggagctg caagataaca aataatcggc actaatgcca ttaacagggc
     4081 ttttgccgaa cgatttcggt cgtgtaatcg tttaataata atagcagaca ggctaaatcc
     4141 tgtaattata agcggtagca cactcaataa agacaattca gccaaattta tccacatagc
     4201 aaccataaac aggaaaagaa agtggattcc aaatccaatc caaaaacctc gtctatttag
     4261 cctaccgtta aaactaaaaa gtgctttata ccaactcatt ttctacccta aagttacatc
     4321 aagtaccatc attactacaa agcctaacat caggcttaaa gtcgcaatat cagtatttcc
     4381 cgaactttgc gattccggaa tcagttcttc tacgaccaca aaaatcatcg ctccggcagc
     4441 aaaggcaaga gcataaggta aaatagccgt catcgacagc acaaaaaccg ccccaatcac
     4501 cgccgcaata ggctcaacca ctgctgacat agagccatac caaaatgctt tcttccgact
     4561 atgcccttcg cctcgaattg gcaatgcaag tgacgagcct tcgggaatgt tttgtaaccc
     4621 tattccgatt gccaaaccga tagctcccat aatggaagca tctacgcctg aaacttgggt
     4681 tgccaaagca ccgaaagcca cccctatggc taatccttct ggaatattat gaatggtaat
     4741 cgcaagaaaa agcaaagtag attttgataa gccttgttta agttgatcat ttccttcagc
     4801 ttgctcaatc ggcttactca agtgcaaatg cggcacaaca tagtcaatta atcgaataaa
     4861 agctccaccg gcaagaaagc caatcgctgc aggcagccaa gctaaagagc cataatccgc
     4921 ctcggcatat tctaaagaag gtgcaagcaa agaccaaaac gaggctgcaa tcattacacc
     4981 cgcggcaaag cccatcatac tgtctaataa ttttcgatta accgatttaa aaaaatagac
     5041 aaatgacgaa ccgaaaatcg tacaacccca agtaaataaa cccgctaaaa aagcttgcca
     5101 tattggagaa agtgaaagga aatattcaaa caagaaatag taccagctaa aataaatatt
     5161 ggctcattct atcaaagatt cagggttatt aatatagtgt agccaaaggc tcaacgaaga
     5221 tttcacttgt aaaacttagc aagaaaataa ccgcttgcat ttccctttca taaatacaat
//
LOCUS       DANKBV010000146         5133 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.146, whole genome shotgun sequence.
ACCESSION   DANKBV010000146 DANKBV010000000
VERSION     DANKBV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.146"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            41..304
                     /locus_tag="PWY43_002148"
     CDS             41..304
                     /locus_tag="PWY43_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2136996.1"
                     /translation="MSKMKSYLYHNLIAIDQLFNALTGGAADETFSSRCYRGAVLAEK
                     PKKRWRFWYAFVNGLFFDKNHCKTAYESEIKRKQYPPEFTEIT"
     gene            406..1587
                     /locus_tag="PWY43_002149"
     CDS             406..1587
                     /locus_tag="PWY43_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDL2136997.1"
                     /translation="MSILDTYLHGVEVVEVNAGGVTISTAATSVIGVVCTGDQADAET
                     FPLNTPVLITNPLNYLEKAGSTGTLRRTLNSIGSIVKTPTVIVRVAESDDNDTLTANI
                     VGTQENGKFTGIKALLTAQSTVFVKPKLLCVPQHDNQAVATELLSVAKKLNAFAFISD
                     NGATTKEQAYTYRQNFSQREGMMIFGDWQSYNTDKKAYDTDYAVARACALQAYIDKTV
                     GWHKNISNVELDGVTGITKPVEFDINESSTEANYLNEKGITICLNHNGFRYWGSRTLA
                     TDTRWAFQQSVRTAQIIKETIGAGLTWAVDMPLTPLRVKTMLEAINNKLRSWASGDDP
                     RILGARVWVAEEITADIIKSGKFVIKYDYHWIPSLESLGLEQRVNDEYVVDLVNTLKA
                     L"
     gene            1596..2102
                     /locus_tag="PWY43_002150"
     CDS             1596..2102
                     /locus_tag="PWY43_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL2136998.1"
                     /translation="MGLPAKLKNFNFSVDGTSYLGETNEVTQPKLAMQLEDYRAGGMI
                     APVGINMGLEKLELEFKTGGHEDDLIKLFGGTISGNAFRFNGAYQHDDDDSVDAVELV
                     CRGRIAEIDEGSSKAGNDTEHSYKASLTYYKKTVNGVDIIEIDTLNQIYIVDGKDRLA
                     EIRKAMGL"
     gene            2183..2497
                     /locus_tag="PWY43_002151"
     CDS             2183..2497
                     /locus_tag="PWY43_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL2136999.1"
                     /translation="MKKVTLKQGILRGEKRITEIEVRKPLTKQLRGTNLTSLMQLSVD
                     EWCIVLPRITTPKLDKADFATMSAADLLKLSGKALDLMSEDFDEADEEDSEEQGKGEI
                     LA"
     gene            2521..2637
                     /locus_tag="PWY43_002152"
     CDS             2521..2637
                     /locus_tag="PWY43_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL2137000.1"
                     /translation="MADIATVFHWTPNAFDEMTIVELGQWREKARLRSGVES"
     gene            2698..3084
                     /locus_tag="PWY43_002153"
     CDS             2698..3084
                     /locus_tag="PWY43_002153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137001.1"
                     /translation="MMILFLIWLADFIGKVHVIINVFFLLAIILCIVGGITFCANSSE
                     YDKAEIKWHNWGKTKVYLAIKVAIASAIIGAIIPSKNTYYAMVGVYVGQEIIANPTSQ
                     RLFDKSIQAIELKLDEVINSDLKKDK"
     gene            3084..3521
                     /locus_tag="PWY43_002154"
     CDS             3084..3521
                     /locus_tag="PWY43_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2137002.1"
                     /translation="MLQQFAMMSLGYFVFMRSTIPYQDTSREMNWSHVQNDVVGAMPK
                     SQFTGKAGETMEISAELRPEITGGKMSILALEMMAEQGSAYPLISGSTFMVLGWFVID
                     RISEQETTFFADGTPRAISFSMSLKRVDDSLLANIIDEVAGFI"
     gene            3521..4762
                     /locus_tag="PWY43_002155"
     CDS             3521..4762
                     /locus_tag="PWY43_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDL2137003.1"
                     /translation="MDFLAELTNHNHRTPAVSVTVRPKPSKDNEGEKAKDISSLITHR
                     LIQLTLTDNRGFEADQLDLELDDTDGLLALPSRGAILSVGLGWQNSPLTYKGEYTVDE
                     LTHDGPPDKVTIRARSADLRGTLTNRHERSFHRTTIGKIVKQIAEENKLKPIVGKEFE
                     NEEVKHIDQTNESSINLLQRLAEQFDAIATVKNGNLIFIKAGNATTASGKPLPLFRIT
                     RSSGDSHSFSIAEGDNYKAVKAYWHNTQTGKRGEVTWDENSQVKKVTKPTMRKKTKVK
                     RGADGKPIKGKDGKSIKETVFVKGKGRQVNAVVQSKPIESDSEAIKTLPHTYISQQSA
                     INACKNHFAKLERGVATFSLTLAEGNAELIPELPVQVSGFKAEIDSNEWIISQVTHSL
                     NKGGGFTTALEMELKPKEEKE"
     gene            4762..>5133
                     /locus_tag="PWY43_002156"
     CDS             4762..>5133
                     /locus_tag="PWY43_002156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137004.1"
                     /translation="MTQNVPYGNPNNLYDDNQKWEGRMSVKKGKVHIYLKHDIRQPFY
                     VLHGACCGYFECTGMIEDWYIYSPVEWEPLANLCQEISSNQRQLWISGLLQVEPSKPC
                     EEKIYRIAVHLVHLRGWSIPGL"
BASE COUNT         1564 a         1091 c         1213 g         1265 t
ORIGIN      
        1 tatttggcgg ggtggacaaa aaactatgag gactttttaa atgagcaaaa tgaaaagcta
       61 tctctaccat aacttaattg ccatagacca actctttaac gctctcaccg gcggagcagc
      121 ggacgaaaca ttttctagcc gttgctaccg tggtgcagtg cttgcggaaa agccgaaaaa
      181 gcgttggcgt ttttggtatg cgtttgtgaa tggcttgttt tttgacaaaa atcattgcaa
      241 gactgcttat gaaagcgaga ttaaacgaaa gcaatacccg ccggagttta ccgaaatcac
      301 ttaatcctgt taaaccgcat ttcacagccc gaaccgctcg caacccgagc ggttattttt
      361 taaagtctta cctgttattt aacccttccc aacacaagga taactatgtc aattttagat
      421 acctatctac acggcgttga ggtggttgag gtcaatgcag gtggtgtcac catttccacc
      481 gctgcaacct ctgtcattgg ggtagtctgc accggagacc aagccgatgc cgagactttt
      541 ccgctgaata cgcccgtttt aattactaat ccgctcaact accttgaaaa agcaggtagc
      601 accggcacgt tacgccgcac acttaattcg attggctcaa tcgttaaaac gccaacggtg
      661 atcgtacgag ttgcggagtc ggacgataac gacaccttaa ccgcaaatat tgtcggcacg
      721 caggaaaacg gcaaatttac cggtattaag gcgttattaa ccgctcaatc aaccgtgttt
      781 gttaagccga aattgctttg tgtgccacag cacgataatc aagccgttgc gaccgaacta
      841 ttaagcgtgg ctaaaaagct gaatgcgttt gcgtttattt cagataacgg tgcaaccacc
      901 aaagaacagg catataccta tcgccaaaat ttctcccagc gtgaaggtat gatgattttt
      961 ggtgactggc aatcgtataa caccgacaaa aaagcctatg ataccgatta tgcggtggcc
     1021 cgagcttgtg cgttgcaagc ctatattgac aaaacagtcg gctggcataa aaacatctca
     1081 aacgtagagc tagacggtgt aaccggtatc actaaaccgg tagagtttga tattaacgag
     1141 agctcaaccg aggcaaacta cctcaacgaa aaaggcatta ccatttgctt aaaccataac
     1201 ggtttccgct attggggttc acgcactctt gccaccgata cccgctgggc gtttcagcag
     1261 tcggtgcgaa cagctcaaat cattaaagag accatcggtg caggcttaac ttgggcggtg
     1321 gatatgccac tcacaccgtt gcgtgtgaaa acaatgctag aggcgattaa caacaagctc
     1381 cgctcttggg catcgggtga tgaccctcgc attttaggtg caagagtttg ggtagctgaa
     1441 gagatcaccg cagatattat caaatcaggc aaatttgtta ttaaatacga ttaccattgg
     1501 attccgtccc tcgaaagcct aggcttagag caacgggtca atgatgaata tgtagtggat
     1561 ttagtcaata cacttaaagc gttatagagg taaaaatggg attaccggca aaacttaaga
     1621 attttaattt ttcggtggac ggcacaagct atttgggcga aaccaatgaa gtgacacagc
     1681 cgaaactggc tatgcaactt gaagactacc gtgcaggcgg aatgattgcc cctgtgggta
     1741 tcaatatggg tttagaaaaa ctggagcttg aatttaaaac gggcggacac gaagacgatt
     1801 taatcaagtt attcggtggg acaatcagtg gtaatgcgtt ccgtttcaac ggtgcatacc
     1861 aacacgatga tgacgattca gtcgatgcgg tggagttagt ctgccgtggg cgaatcgctg
     1921 aaattgacga aggctcaagc aaagccggta atgataccga gcatagctat aaagcctcgc
     1981 taacctatta caaaaaaacg gtgaatggtg tcgatattat cgaaatcgac accctcaacc
     2041 aaatttacat tgtcgatggc aaagaccgct tagcggaaat tcgcaaggca atgggtttat
     2101 agatttcccc ctaaattttc cccaaaagcc ccttcccctt ttttcaaggg gcttttaacg
     2161 ataaataatt aaggattaaa caatgaaaaa agtaacatta aaacaaggca ttttgcgtgg
     2221 tgaaaaacgt attaccgaga ttgaggtgcg taagccatta accaaacaac tgcgtggcac
     2281 aaatcttacc tcattaatgc agttaagcgt tgatgagtgg tgtattgtac tgccacgcat
     2341 taccacgcca aaattagaca aagccgattt tgccacaatg tcggcagcag atttacttaa
     2401 actttccggc aaagccctag atttaatgag cgaagacttc gatgaggcag acgaagagga
     2461 cagcgaagaa cagggaaagg gcgagatttt agcctaattc cacagtttgt tgatgacgct
     2521 attgctgata ttgctaccgt gtttcattgg acacccaatg cctttgatga aatgacgatt
     2581 gtcgaactcg ggcaatggcg agaaaaagcc cgcttacgaa gtggggtaga aagttaaaac
     2641 aagcggtcgt gtttcgcaaa aaatttgcaa aattcgaccg cttttagaaa ggtgtttatg
     2701 atgatcttat ttttaatttg gctcgctgat tttattggga aagttcacgt tatcattaac
     2761 gtatttttct tgcttgcgat tattttatgt attgttggag gtataacctt ttgtgctaac
     2821 tccagtgagt acgataaggc agaaataaaa tggcataatt gggggaaaac aaaagtttat
     2881 cttgccatta aagtagcgat tgcatctgcg attattggag cgattatccc ttcaaaaaat
     2941 acctattatg caatggtggg agtatatgtc gggcaagaga tcatcgctaa tccgacctcg
     3001 caaagattat ttgataaatc tattcaagcc attgagttaa aacttgatga agtgattaat
     3061 tcagacttaa aaaaagataa gtaatgctcc aacaattcgc aatgatgtcg ctaggctatt
     3121 ttgtgtttat gcgatcgaca atcccttacc aagacaccag ccgagagatg aactggtcgc
     3181 acgttcaaaa tgatgtggtt ggggcaatgc caaaaagcca atttaccggc aaggcagggg
     3241 agacaatgga gatttcagcc gagctacgcc ctgaaattac cgggggcaaa atgtcaattc
     3301 ttgcccttga gatgatggca gagcaaggct cggcttatcc gttgattagt ggttcaacct
     3361 ttatggtgct aggctggttt gtgattgatc gaatcagcga gcaggaaacc accttttttg
     3421 cagacggcac tccccgagcg attagctttt cgatgtcgct taaacgggtt gatgatagtc
     3481 tgctcgccaa tattattgat gaggtcgcag ggtttattta atggattttt tagcagagct
     3541 aaccaatcat aaccaccgca caccggctgt ttcggtgacg gttcgcccaa agccaagcaa
     3601 agataatgag ggcgaaaaag ccaaagatat ttccagcctg attactcacc gcttaattca
     3661 acttacctta accgacaacc gaggctttga ggcagatcag ctggatttag agctggacga
     3721 cactgatggc ttactcgctt tgccaagtcg aggagcgatt ttatcggtcg ggttaggctg
     3781 gcaaaacagc cccctgacct acaagggcga atacacagtg gacgagctaa cccacgacgg
     3841 cccgccggat aaagtgacga ttcgggcaag aagtgccgac ttgcgaggca cgctgaccaa
     3901 tcgacacgag cggagctttc accgcacgac tattggtaaa attgtgaaac agatcgccga
     3961 agagaacaaa ctcaagccga tagtgggcaa ggagtttgaa aacgaggagg tgaaacacat
     4021 tgaccaaacc aacgaaagct ctattaatct cttgcaacgc ctcgcagaac aattcgatgc
     4081 catcgccact gtgaaaaacg gcaatttgat tttcattaaa gccggaaacg ccacaaccgc
     4141 aagcggtaaa ccgttacccc tgtttcgcat tacccgcagt tcgggggatt cccacagttt
     4201 ttcgattgcc gaaggggata actacaaagc ggttaaagcc tattggcaca acacgcaaac
     4261 cggcaagcga ggcgaggtga cgtgggacga aaacagccaa gttaaaaaag tgactaagcc
     4321 cacaatgcgg aaaaagacca aagtaaaacg tggagcagac ggcaagccaa taaaaggcaa
     4381 agacggtaaa agcattaaag agaccgtctt tgtaaaaggc aagggcagac aagttaatgc
     4441 ggtggtgcaa agcaaaccga ttgaaagcga cagtgaggca attaaaaccc tacctcatac
     4501 ctatattagc cagcaatcag cgattaatgc gtgtaaaaac cattttgcaa agcttgagcg
     4561 aggtgtggca acttttagcc taactcttgc cgagggcaat gccgagctta tccccgaatt
     4621 accggttcag gtttcaggct ttaaggcgga gattgatagc aatgagtgga taatctcaca
     4681 ggttacgcac agtttaaata aaggtggtgg gtttacaaca gcgttagaga tggagttaaa
     4741 accgaaagag gagaaagagt aatgacgcaa aatgttccct acggaaaccc aaacaacctt
     4801 tatgacgaca accaaaaatg ggaaggcaga atgagcgtga aaaaaggtaa ggttcacatc
     4861 tacctcaagc acgatattcg ccaaccgttt tatgtgttac acggtgcttg ctgtggatat
     4921 tttgaatgca ctggaatgat tgaggactgg tatatttaca gccctgttga atgggaaccg
     4981 cttgctaacc tatgccaaga aatttcttca aaccagcggc aactatggat ttcagggctt
     5041 cttcaggtag aaccttcaaa accgtgtgaa gagaagattt atcggattgc agtgcattta
     5101 gtacatctaa gagggtggag tattccaggc ctt
//
LOCUS       DANKBV010000147         5132 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.147, whole genome shotgun sequence.
ACCESSION   DANKBV010000147 DANKBV010000000
VERSION     DANKBV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.147"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..329)
                     /gene="trxA"
                     /locus_tag="PWY43_002157"
     CDS             complement(<1..329)
                     /gene="trxA"
                     /locus_tag="PWY43_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2137005.1"
                     /translation="MSNVIHTTDATFEQDVLKSDVPVLLDFWAPWCGPCRMIGPVLDD
                     LSVDAQFDGKVKIVKMNVDENPSVPAQFGVRSIPFLLLFKNGEVIAQQVGAIPHAQMA
                     EFIKQAL"
     gene            complement(576..1241)
                     /locus_tag="PWY43_002158"
                     /pseudo
     CDS             complement(576..1241)
                     /locus_tag="PWY43_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
     gene            1496..4063
                     /gene="clpB"
                     /locus_tag="PWY43_002159"
     CDS             1496..4063
                     /gene="clpB"
                     /locus_tag="PWY43_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL2137006.1"
                     /translation="MNFEKMTTKLQEALAESQSLAVGKDNPYIEPAHLLYALVKQDGG
                     SVAPLFTALNINVQTLISELQAILDRLPQVQGGNTQVSQQLVRLLNQSDKLAQQFGDS
                     FISSELFVLASIEDNGDLGKLWKKFGLSKEKLTQAISQIRGGETVNNQNAEETRQALQ
                     KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPEGLKNKRVLSLDMGALIASAKYRGEFEERLKAVLNELAKEEGQVILFID
                     EIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQYIEKDAALERRFQKV
                     LVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYVSDRQLPDKAIDLI
                     DEAASSLRMEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEEMAAKE
                     REYSELEEIWKAEKSALLGTQHIKSELENARIAMDQARRENDFEKMSELQYGVIPNLE
                     KQLQAVENGEKNADAQLLRTKVTEEEIAEVLSKATGIPVAKMMEGEKEKLLRMEEVLH
                     ARVIGQNEAVDAVTNAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELSKTLANFLFDD
                     PDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRHPYSVVLLDEVEKAH
                     PDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHLIRENAYKGYDVVKEMVM
                     EVVGQHFRPEFINRIDETVMFHSLGQEHIRSIAKIQLQRLIQRLAERGYEVTVTDAAL
                     DHIGKAGFDPLFGARPLKRAIQQELENPLAQQILSGKLLPNKAVTVDYQNGQIVATQ"
     gene            4277..>5132
                     /locus_tag="PWY43_002160"
     CDS             4277..>5132
                     /locus_tag="PWY43_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2137007.1"
                     /translation="MSQLFPEATIRTQAPYRNDIVGSFLRTDALKKAREDFNQQKITR
                     DELREVTRQEVKKLVELQKQHGVKAVSDGEFSRTWWHLDFLAELDGMDWQETEVFNVN
                     FTGHKPKGQTVKIVGDIDFSESHPFVDAYRLLKEAAGDYPTKFTIPAPTMLHIICCVR
                     AEDYQPLPQYQDEQKLYDDIANAYIKAMHKFYDMGLRNLQLDDTSWGQFCAEDKRKEF
                     AARGFNLTDLAEKYVALLNRIVDAKPADMSITMHICRGNFRSSWFSEGGYGPVAEVLF
                     DKCRVDGFF"
BASE COUNT         1621 a         1019 c         1112 g         1380 t
ORIGIN      
        1 tataatgctt gtttgataaa ttcagccatt tgtgcgtgcg gaattgcacc aacttgctgg
       61 gcaatcactt caccattttt gaataatagt aaaaatggaa tgctgcgtac gccaaattgt
      121 gctggtacag atggattttc atctacgttc attttgacaa tttttacttt accatcaaat
      181 tgtgcatcaa cagataaatc gtcaagtacg gggccgatca tacggcaagg accacaccaa
      241 ggcgcccaga agtctaataa taccggtaca tctgatttta aaacatcttg ttcaaatgta
      301 gcatctgttg tatgaatgac attgctcata agtttatcct taaatattaa attaaaaaaa
      361 gaaacgccct aactggcaag ttaaggcgaa cagttgtgta taagatagag attaatctat
      421 aaaatttcaa gcaagattta ccaattacac caataaatat ttgcatagtg agattttatt
      481 atctcttcaa ttaaaagtag aaacgggcta tatctaaaca actgcaacaa aaagggctaa
      541 tcactaagat tagcccaact cgttctcaac aataattaga acttcacacc gaaaccggca
      601 aataggacaa ccggatcaag ttttactttt acttcgtgtt gcatatcatt agttacgctt
      661 ggcgttttga atttcgccgt ggttttgata cgggtgtacc acgctgaggc gttgaaatag
      721 atgttatcgg ttaatttcac atcaacaccg agattcgcca ccggtccgaa agagtgtttt
      781 ttcacattta aatccgttac cgctgcatgt actgattttg cgtggtaaaa acgggtatag
      841 ttcagacccg caccgatata aggtttcact ttagcggttg gttcaaagaa atagtattga
      901 gcataaaggc tcggtggtaa ttgtcttaag ctcacaacac gacctaaatt cgcatttgca
      961 cctaatgccg gcacaatcgc attgatttaa tgcgagaatg gcgttgcacc taataattcg
     1021 atacccacat tatccgccaa catataagtg gccgttaaac ctaactgggt gttgtttttt
     1081 acttttaaat caatatcaac cgcagttttc gtatctgaac ttgaatttgc cttaacatgc
     1141 acagcaccgg cacggaatat cacatcacca gcttgatgtg ccatcgcagt tccagttaat
     1201 aatgccccac ctaaaataat tgctaaagtt gattttttca ttttcatatc ctcaaaaata
     1261 attagaatac ctataatgag gtaaacttta gcagagtgta aatgaaatga aatagaaatg
     1321 aattgatatt tatcaaactt ttaacacaaa gatacccaac aagagttatt ttatttattt
     1381 ttgtgatctt tttcacattt ttacttgaaa attatttacc aagctccatt taatgtttca
     1441 acaagagatg taaataaaaa tttacacttt ctaaactcat tgtaaggaga aagaaatgaa
     1501 ttttgaaaaa atgaccacaa aactgcaaga agccttggca gaatcccaat cgcttgcagt
     1561 tggaaaagat aatccttata tcgaacctgc ccatttactg tatgcgttag taaaacaaga
     1621 tggtggatct gttgcccctc tctttaccgc attaaatatc aatgtgcaaa ccttaattag
     1681 tgaattgcaa gctattttag accgcttgcc gcaagttcaa ggcggtaata cacaggtttc
     1741 acagcaatta gtgagattgt taaatcagtc agacaagctc gcacaacaat ttggcgacag
     1801 ttttatttcc tctgaattgt ttgtgttggc aagcattgag gataatggcg atttaggtaa
     1861 attatggaaa aaatttggct taagcaaaga aaaactgaca caagctatca gccaaattcg
     1921 aggaggagaa accgtgaata atcaaaacgc agaagaaacc agacaagcat tacaaaaata
     1981 tacgattgat ctgaccgagc gtgccaaagc tggcaagcta gatccggtaa tcggtcgtga
     2041 tgaagaaatc cgccgtgcgg tacaagtttt acaacgccgc actaaaaata acccagtatt
     2101 aatcggtgaa ccgggggtcg gtaaaactgc gatcgtagaa ggcttggcac agcgtattgt
     2161 aaacggcgaa gtgccggaag gcttgaaaaa taagcgtgta ctttcgctgg atatgggagc
     2221 attaattgcc agtgcgaaat atcgtggtga atttgaagag cgtttaaaag cggttttaaa
     2281 cgaactggcg aaagaagaag ggcaagttat cctctttatt gatgaaattc acacgatggt
     2341 tggagcaggc aaaacagacg gagcaatgga cgccggcaac ttattaaaac caagccttgc
     2401 ccgtggtgaa ttgcactgtg tgggggctac aacccttgat gaatatcgcc aatacattga
     2461 aaaagatgca gcccttgaac gccgtttcca aaaagtattg gtggacgaac caacggttga
     2521 agatacgatt gcgattttac gtggtttgaa agagcgttat gaaatccatc atcacgttca
     2581 aattacagac cctgccatcg tggcggcggc aacgctttcg caccgttatg tgtctgaccg
     2641 tcaattgccg gataaagcga ttgacttgat tgatgaagcc gcttcaagcc tgcgtatgga
     2701 gattgattct aaacctgagc cgttggataa attagaacgc cgcattatcc aactcaaatt
     2761 ggaacgccaa gcgttacaaa aagaggaaga tgaagcgagc cgtcagcgtt tagccaaatt
     2821 agacgaagaa atggcagcga aagagcgtga atattccgag ctggaagaaa tctggaaagc
     2881 ggaaaaatcc gccttacttg gtacacagca tattaaatcg gagttggaaa atgcccgaat
     2941 tgcgatggat caggctcgcc gtgaaaacga tttcgagaag atgtcggaat tgcaatatgg
     3001 cgtgattcca aacttggaaa aacagttaca agcggtcgaa aatggcgaaa aaaatgcaga
     3061 tgctcagctc ctccgtacta aagtcaccga agaggaaatt gcggaagtgc tttcaaaagc
     3121 aacgggcatt cctgttgcaa aaatgatgga aggcgagaag gaaaaactcc ttcgtatgga
     3181 agaagtatta cacgctcggg ttatcgggca aaatgaagcc gttgatgcgg tgacaaatgc
     3241 gattcgccgt agccgtgccg gactttccga tccgaataaa ccgattggtt cattcctatt
     3301 cttaggaccg accggcgtgg gtaaaaccga gttaagcaaa accttagcta atttcttgtt
     3361 tgacgatcct gatgctatgg tgcgaattga tatgtctgaa tttatggaaa aacacagcgt
     3421 atcccgctta gtgggcgcac ctccgggcta tgtcggctac gaagaaggcg gctatttgac
     3481 cgaagcggtt cgccgccatc catattcggt agtgttactt gatgaagtgg aaaaagctca
     3541 ccctgatgtg tttaacattt tattgcaagt gttagatgac gggcgtttaa ccgacgggca
     3601 aggcagaaca gtcgatttcc gcaatacggt agtgattatg acctcaaact taggctcgca
     3661 tttaattcga gaaaatgcgt ataaaggcta tgatgtggtt aaagaaatgg tgatggaagt
     3721 cgtcggacag cacttccgcc ctgaatttat caaccgtatt gatgaaacgg tgatgttcca
     3781 ctcacttggt caagagcata tccgttcgat tgcgaagatt cagctacaac gcttaatcca
     3841 acgcttggca gaacgaggct atgaggtgac agtaaccgat gcagcattag atcatatcgg
     3901 caaggctggt ttcgatccgc tgttcggagc aagaccactg aaacgtgcga ttcaacaaga
     3961 gcttgaaaat ccgcttgctc aacagatctt aagcggtaaa ttattaccga ataaagccgt
     4021 tacggtggat taccaaaacg ggcagattgt tgcaacacaa taatgtctcc gatggtgcga
     4081 gcgtcctcgc tcgtacctta caagcggtgg atttatcgta aaaatttgca aaaaattgct
     4141 cgaaaatgac cgcttgcaca gtaaaaattt tcatattgcc cttcattaat tttcataagc
     4201 taatgaaggg ctttttatta ctctttttgc tatcattaac ggcgtttata accaatacaa
     4261 ttacaaggag taatttatga gccaattatt tccggaagca acaatccgca ctcaagcccc
     4321 gtatcgtaat gatattgtag gcagctttct acgtaccgat gcgttaaaaa aagccagaga
     4381 agatttcaat cagcaaaaaa tcacccgtga cgaattgcgt gaagtcacac gccaagaggt
     4441 caaaaaatta gttgagttgc aaaagcaaca cggtgtaaaa gcagtctctg acggtgaatt
     4501 tagccggact tggtggcatt tagatttctt agctgaactt gatggaatgg attggcaaga
     4561 aaccgaagtc tttaacgtga actttaccgg tcataaaccc aaagggcaaa cggtaaaaat
     4621 cgtgggcgat attgatttct ccgaaagcca cccttttgtg gacgcttatc gtcttttaaa
     4681 agaagctgcc ggcgattacc caaccaaatt cacgattccg gcaccgacaa tgttacatat
     4741 tatctgctgt gtgcgtgcgg aagattacca accgttacca caatatcaag acgaacaaaa
     4801 actgtacgat gatattgcta atgcttatat caaagcaatg cacaaattct acgatatggg
     4861 gctacgtaac ttacaattag acgacaccag ttggggacaa ttctgtgccg aagataaacg
     4921 gaaagagttt gcagccagag gatttaattt aactgattta gcggaaaaat atgtcgcatt
     4981 attaaaccgt attgttgatg caaaaccggc ggatatgtca attacgatgc acatctgccg
     5041 tggtaacttc cgttcttctt ggttctcgga aggcggttat ggaccggttg ccgaagtatt
     5101 attcgataaa tgccgtgttg acggcttctt ct
//
LOCUS       DANKBV010000148         5039 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.148, whole genome shotgun sequence.
ACCESSION   DANKBV010000148 DANKBV010000000
VERSION     DANKBV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.148"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(87..872)
                     /gene="znuB"
                     /locus_tag="PWY43_002161"
     CDS             complement(87..872)
                     /gene="znuB"
                     /locus_tag="PWY43_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDL2137008.1"
                     /translation="MFDILFPAWLAGVLLAFITAPLGAFVVWRKMAYFGDTLSHSALL
                     GVALGIFLELDPYLAVIGMTILLALLLVWLEQKTNYSVDTVLGIIAHTSLSLGVITIS
                     LLDNVRVDLMSYLFGDLLAINFSDVVFIGIGATLICLILMLFWKRLLSITISPELAQI
                     EGLNVARLRLLLMILTALTIALSMKFVGALIITSLLIIPSATARRFAKTPEKMVLIAI
                     LFSIVAVTGGLLLSGWKDTPAGPSVVICAGLIFLLSLFKKEYH"
     gene            complement(959..2296)
                     /locus_tag="PWY43_002162"
     CDS             complement(959..2296)
                     /locus_tag="PWY43_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL2137009.1"
                     /translation="MSSLSFDFSEDFRPLAAKMRPRNLAEYVGQSHLIGEGKPLRRAI
                     EAGHSHSMIFWGPPGTGKTTLAEIIAHHLDAEVERISAVTSGVKEIREVIEQAKLNRQ
                     AGRRTLLFVDEVHRFNKSQQDAFLPHIEDGTIIFIGATTENPSFELNNALLSRARIYI
                     LKPLQAVEILKILQMAIADTERGLGNETLVLEDDVLALLADYVNGDARFALNCLELMV
                     DMAQDSAKGKVLNKSLLTEVLGERQARFDKGGDRYYDLISALHKSIRGSSADGALYWY
                     ARILTAGGDPLYVARRLLAIASEDIGNADPRAMQIAIVAWDCFTRVGAYEGERAIAQA
                     IIYLAVAPKSNAVYQAFNEAKRLAKEAKDYDVPAHLRNAPTQLMKSLGYGEEYRYAHN
                     EPNAYAAGENYFPPELKDTEFYFPSERGMEKQIKEKMAWLKAQDQASPIQRYK"
     gene            complement(2306..2794)
                     /gene="mreD"
                     /locus_tag="PWY43_002163"
     CDS             complement(2306..2794)
                     /gene="mreD"
                     /locus_tag="PWY43_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL2137010.1"
                     /translation="MRTNPIFQILVIISIFVVAFVLEIMPWPVGFQGLRPTWVVLVLI
                     YWALALPDKMSVGTAFIAGIVWDLILGSILGIHALVLSVAIYFVAKYHLILRNLSLWL
                     QSLLVMAYIVLIRFFIFVIEYILHSAEFNSQELLGAVISGVLWSWIFLLMRHIRRQLR
                     LR"
     gene            complement(2794..3849)
                     /gene="mreC"
                     /locus_tag="PWY43_002164"
     CDS             complement(2794..3849)
                     /gene="mreC"
                     /locus_tag="PWY43_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL2137011.1"
                     /translation="MKPIFAKAPSLGMRLIIAVIFSIMLIISDGRSSLMIQARNLLET
                     AVSGLYYFANTPRSILDGVSGNFIDNNKLQLENNLLKEQLREKSADLLLLDQLKVENQ
                     RLRLLLSSPLRQDEYKKIAEVLAAEMDAYRQQVVINQGRLDGAFVGQPIIDERGVVGQ
                     VISVGEKSSRVLLLSDITHAIPVQVLRNDVRGIASGTGVNNELVIDNLPRSIDVVKGD
                     VLVTSGLGGRFPEGYPVAIVETVENDTKSQFARIVARPLASFDRLRYLLLLWPTAEEK
                     NYSQSLSPAQVREVVEERRNSVNPFERLKQMSNKKTEVQLEAKEEQIENDEINENPAA
                     QPEAENEQNYHTEEGAE"
     gene            complement(3951..5003)
                     /locus_tag="PWY43_002165"
     CDS             complement(3951..5003)
                     /locus_tag="PWY43_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDL2137012.1"
                     /translation="MFKKFLGLFSNDLSIDLGTANTLVYVKGQGIVLDEPSVVAVRQD
                     RMGSLKSIAAVGSHAKLMLGRTPKSIMAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPAGATQVERRAIKESAIGAGAREVYLIEEPMAAAIGAGLPVHEAMG
                     SMVIDIGGGTTEVAVLSLNGIVYSTSVRIGGDKLDEAIIAYVRRHFGSAIGEATAERI
                     KQEIASAKIESDDDVKTMEVHGHNLAEGAPRTFELNSRHVLEAIEQPLNGIVEAVKSV
                     LEQCPPELAADIFERGMVLTGGGALLHNLDRLLSDSTGVQVVVAEDPLTCVARGGGKA
                     LEMIDMHGGDVFSIDD"
BASE COUNT         1572 a         1179 c          884 g         1404 t
ORIGIN      
        1 taatagctac atttaaacag attgattaga aaattcaatg ccctttacat aaaaatctta
       61 tgctttattt tcatacatta ttctgtttaa tggtattctt ttttaaatag cgataatagg
      121 aaaatcaagc cggcacaaat gactaccgat ggtcctgccg gagtatcttt ccagccggat
      181 agcaataaac cgccggttac tgcaacaata ctgaataaaa ttgcaatcag caccattttt
      241 tcaggcgttt tagcaaaacg tcttgcagta gcggaaggaa taatcagcag tgaggtaata
      301 attaaagcac cgacaaattt catacttaat gcgatagtta atgcggttaa aatcatcagc
      361 aataaacgta agcgagccac atttaatcct tcaatttgag caagttccgg gctgatggta
      421 atcgacaaca accgcttcca aaacagcatt aaaatcaaac aaattagggt tgctccgatt
      481 ccgataaaca ccacatcaga aaaattaatt gccagtaaat cgccgaataa gtaagacatc
      541 aaatccactc gtacattatc caacaaacta atggtaatta ccccgagaga aaggctagta
      601 tgggcgataa tccccaacac ggtatccacc gaataattgg ttttttgctc caaccaaacc
      661 agcaataatg caagaagaat agtcattcca attacagcaa gatacggatc gagttctaaa
      721 aaaatcccta atgccacgcc gagcaatgca gagtgcgaaa gtgtgtcgcc gaaatacgcc
      781 atttttcgcc acaccacaaa agcccctaga ggtgcggtaa taaaggctaa caaaacaccg
      841 gcaagccaag ccggaaataa aatatcaaac atattggttc tcaaaggcaa gtaaaacaag
      901 cggttatttt ttcggaaaaa tttgcaaatt ttttgctgat ttagaccgct tgtcatattt
      961 atttataacg ttgaatcggg ctggcttgat cctgtgcttt cagccacgcc attttttctt
     1021 taatctgttt ttccattccc cgctcacttg ggaagtagaa ttcggtatct ttcagctcag
     1081 gcgggaaata attctccccg gcggcataag cgtttggctc attgtgggca tagcgatact
     1141 cttcgccata gcctaaagat ttcattaatt gagtgggagc attacgcaaa tgcgccggca
     1201 catcgtagtc ttttgcctct ttagcgaggc gctttgcttc attaaaagct tgatagaccg
     1261 cattgctctt tggggcaact gccaaataaa tgatcgcttg ggcaatcgct ctttcacctt
     1321 cataagcccc cactcgggta aagcaatccc aagccacaat cgcaatttgc attgctcgag
     1381 ggtcggcatt accaatatct tcagaagcaa tcgcaagcag ccttcttgcc acataaagcg
     1441 gatcaccacc ggcggttaaa attcgggcat accagtataa tgctccatca gccgatgagc
     1501 ctcgaatgga tttatgtaac gcagaaatga gatcatagta acgatcaccg cctttatcaa
     1561 aacgagcttg tcgttcgcct aacacttcgg tcagcaaact tttattcagc actttccctt
     1621 tagctgaatc ttgtgccata tctaccatta attccaaaca gttcaaggca aaacgggcat
     1681 caccattaac ataatcagca agcaatgcca gtacatcatc ttcaagtacc aaagtttcat
     1741 tgcctaaacc acgttcggta tcggctatcg ccatttgcaa aatttttaga atttcgaccg
     1801 cttgtaacgg ctttaaaata taaattctgg ctcgggaaag cagtgcgtta tttagctcaa
     1861 aagagggatt ttcggtagtc gcaccaataa aaataatagt gccgtcctca atatgcggta
     1921 aaaacgcatc ttgctggctt ttgttaaagc ggtgaacttc atccacaaaa agcaacgttc
     1981 gtctgccggc ttggcggttt aatttggctt gttcaatcac ctctcggatc tcttttacac
     2041 cgctggtcac agcggaaata cgttcaactt ccgcatcaag atgatgggcg ataatttcag
     2101 ctagggtcgt ttttccggtg ccgggcggtc cccaaaaaat catagaatga ctatgtccgg
     2161 cttcaatcgc tctgcgtaac ggtttgcctt cgccaattaa atgagattgc ccgacatatt
     2221 cagccaagtt tcgaggtcgc attttggctg caagcggtcg aaaatcttca ctaaaatcaa
     2281 aagacaaact actcatttta acctcttaac gtaaccgcag ttggcgacga atatgacgca
     2341 ttaataagaa aatccaagac caaagcacac cactgattac agcacctaac aattcctgtg
     2401 aattaaattc ggcactgtgt aaaatgtatt caatcacgaa aataaagaaa cggatcagca
     2461 caatatatgc cattactaat aggctttgta gccatagcga taagtttcgc agaatcagat
     2521 ggtatttcgc aacaaaataa atcgccaccg ataacactaa ggcgtggatg cctaaaatcg
     2581 agccaagaat cagatcccag acgatgccgg caataaaagc tgtccccaca ctcattttat
     2641 caggtaatgc caatgcccaa taaattaaca ccaaaacgac ccaagtaggg cgtagccctt
     2701 ggaatcctac cggccaaggc ataatctcaa gtacaaaagc aaccacaaaa atcgaaatta
     2761 tcacaaggat ctgaaaaatc gggtttgttc tcattattcc gctccttctt ccgtatgata
     2821 gttttgttca ttttccgctt ctggttgtgc tgccggattc tcatttatct catcattctc
     2881 tatttgttct tcttttgcct ctagctgcac ttcagttttc ttgttagaca tctgttttaa
     2941 acgttcaaat ggattcacgc tattacgtct ttcttcaaca acttcacgta cttgcgctgg
     3001 cgacaagctt tgggagtagt ttttctcttc cgccgtcggc caaagcagta ataagtaacg
     3061 taagcgatca aatgaggcta acggacgagc aacaattctg gcaaattgac ttttggtatc
     3121 attttcaacc gtttctacaa tcgccaccgg atatccctca gggaaacgtc cacctaagcc
     3181 tgaagtcacc agcacatcac cttttaccac atcaatagaa cgaggcaagt tatcaatgac
     3241 caattcatta ttcacccctg taccactggc aatgccacgt acatcattgc gtagcacttg
     3301 aacaggaata gcatgagtaa tatcagagag caataaaaca cggctagatt tttcccctac
     3361 agaaataact tgtccaacaa cacctctttc atcaataata ggttgcccga caaaagcacc
     3421 gtctaaacgc ccttgattaa tcactacttg ctgacgataa gcatccatct cagcagctaa
     3481 tacttcagca atttttttat actcatcttg ccgaagcgga gaactcaata acaatcttaa
     3541 acgctgattt tcaaccttta gctgatcaag caataataaa tcggcacttt tttctcttag
     3601 ctgctctttt aataaattat tctcaagctg taatttatta ttatcaataa aattgccact
     3661 taccccatct aaaattgaac ggggggtatt agcaaaataa tataatccac ttaccgcggt
     3721 ttctaataga ttacgcgctt gaatcattaa agagctacgc ccatcggaaa taatcagcat
     3781 aatagaaaat atcactgcga taatcaggcg catccctaaa gagggagctt tagcaaaaat
     3841 aggtttcatt agagaaatat atgtgaattt attgggaatc tataacgaaa attgacaata
     3901 gaatcaatcc attgccaacc tccatttaaa cagctgctaa gagaaatgta ttaatcatca
     3961 atactaaata catcaccacc gtgcatatcg atcatttcta atgctttacc accaccacga
     4021 gctacacaag ttaatggatc ttctgctact acgacttgca cgccagttga atcagaaagt
     4081 aaacgatcta aattatgtaa taatgcacca cctcctgtta acaccatacc gcgctcaaaa
     4141 atgtctgctg ccaattcagg tgggcattgc tctaagactg atttaactgc ctctacaata
     4201 ccatttaatg gttgttcaat tgcttctaat acatgtcttg agttaagctc gaaagtacga
     4261 ggagcgcctt cagctaaatt gtgtccgtgt acttccattg ttttaacatc atcatcggat
     4321 tcaatcttag cactagcaat ttcttgttta attcgctctg ccgttgcttc accaatagca
     4381 gaaccaaaat gacgacgtac ataagcaata atagcttcat ctaacttatc accaccaata
     4441 cgcacagaag ttgaatacac aatgccgttt aaagaaagca ctgccacttc ggttgtaccg
     4501 ccaccaatgt caattaccat tgatcccatt gcttcgtgaa ccggcaaacc tgcaccgatt
     4561 gcagccgcca ttggctcttc aattaaatag acttcacgcg ctcctgctcc aattgcagat
     4621 tctttaattg cacggcgttc cacttgtgtt gcacctgctg gcacgcacac taatacacga
     4681 gggcttgggc gcataaagtt attactatgt acttgtttaa taaagtgttg caacattttt
     4741 tctgttacaa aaaaatccgc aatgacacca tctttcattg gacgaatcgc cattatgctt
     4801 tttggtgtac ggcctagcat taattttgca tgtgagccta ccgccgcaat actttttagc
     4861 gatcccatac gatcttgacg tacagcaacc actgagggtt catctagcac aataccttga
     4921 cctttaacat aaaccagtgt atttgctgtc ccgagatcga tagaaagatc gtttgaaaat
     4981 aatcctaaaa attttttaaa cataaaagaa tccgttaaaa taaaattatt caaaaataa
//
LOCUS       DANKBV010000149         5008 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.149, whole genome shotgun sequence.
ACCESSION   DANKBV010000149 DANKBV010000000
VERSION     DANKBV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.149"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..3145
                     /locus_tag="PWY43_002166"
     CDS             <1..3145
                     /locus_tag="PWY43_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL2137013.1"
                     /translation="ENTIEQSAIATLQGLGWDYTYGKTILAGLEHEWRERATEVVLKP
                     LLAEAIAKFNPNLPACEVENVVAQVCRAESSDLAERNRQAYDWLRNGVKITYQLDGEQ
                     VSDVVQLIDFQRPENNDFRIVNQLDIGGKKGKRIPDLIGFVNGLSLVVFELKNPLKEN
                     ADIGKAFAQLQTYKDEISDLFVFNQALVISDSIVARIGSLTADFDRFTPWRVVDEKNQ
                     NKRIVFEDELTALLQGVMNPKNLLDYVQNFVVFERDGKNRLIKKIGAYHQFYGVNEAV
                     DCTLLATTGNRKIGVFWHTQGSGKSLSMLFYAGKVLSQGCLKNPTLVLVTERNDLDGQ
                     LYATFCGGEALLKQTPIQADGRDELRSALASRSAGGVIFTTIQKFGLMEGELAHPVLN
                     ERENIIVITDEAHRSQYGFSQKINHKGEYREGYAKHLRSALPNASFIGFTGTPIELDD
                     KDTQEVFGKYVSIYDFEDAVEDGATVPIIYEPRQISLGESGEFTKVMEEAQQLIDDDE
                     NSYNFRLREKLHSVDSRLQKMAEDIIAHYDERTKQQDGKAMVVVMSRAICVKLYEKIT
                     ALRPEWHSNDVLQGSIKIVMTSNASDPAEWQKHNQDKKTLEKRFKDPDDPLNIVIVRD
                     MWLTGFDAPCCNTMYIDKPMSGHNLMQAIARVNRVFRNKSRENGGLIVDYVGLTDELE
                     KAMKQYTNAGGKEKPVRDISAVLEKMVEHITVIRGQFATPIDGQAVDIAKMLQISEPP
                     KLLNAILTSANHILALDRIQPPDNTAKDKTPRKNAFLQSVRLAKKGYALCGALKAVEP
                     YKQELAFYDAVRATIIKNSTAPRNSSSENDRLLQLTALMNRAVQSDGVVDLFDLLKKD
                     RPNINLLSDEFLETVKNSPTKDLWLSAMERYLASQLREQSGANLATKKAFEQKLKEAM
                     NQYHNHNLSVLEILEELIALAKEFEARQKRGEALGLSPAEMAFYDALARNESAVREMG
                     DEVLMKLAKDITDKLRKSVTVDWQYKDSVRAKMRTLIRIALRTYKYPPDLQAEAIEFV
                     LQQAEEIAGEFAITETA"
     gene            complement(3179..>5008)
                     /gene="glnE"
                     /locus_tag="PWY43_002167"
     CDS             complement(3179..>5008)
                     /gene="glnE"
                     /locus_tag="PWY43_002167"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDL2137014.1"
                     /translation="NLLTQTQVETLHNAYIFYRQIENVLQAIDDKQTQTLPTDEASQA
                     RLVFACQSYYQQDLYSEKTHWVEHSFNDWQQFMAVLSQYQQAVRQIFNEIIGEEETQA
                     NCNPVNEKLAEWKDILHYNIRLEDLSAVLKGYPKVAENDYTEIFRHFSTTFQDWVKRP
                     IGVRGREVLRNLMPRIADSIFKGEDHLLLLPRVLNIVDKITTRTTYLELMLEKEQILP
                     QLLALCSKSVMIAEQIARYPMLLDELMSHRGLTDVQAFEKYQSALQDYLIRIPEEDEE
                     ALIDGLRQFKQTQILRIAAADILGVLLVMKISDHLTYLAEAIINAVVNMAWKQVSQRF
                     GVPEHLEADEKGFAVIGYGKLGGIELGYNSDLDLVFLHNAPEDSQTVGGKKEISSHQF
                     YLKLAQKINSIFNLNTSAGVLYEVDMRLRPSGEAGLLVSTFNAYEHYQKNEAWTWESQ
                     ALVRTRCVFGAENLKQAFEKIRQSTLAQPRASGQLRQEICEMRQKMYQHLSSHSAEQF
                     HIKQDQGGITDIEFIAQYLVLAHSHQHPKMAVWSDNVRIFDSAVECGILSSEQSEQLQ
                     HCYTALRNKIHHLNLLRKDSVVDASEFTTERAFVREMWQRLLA"
BASE COUNT         1402 a         1090 c         1181 g         1335 t
ORIGIN      
        1 cgaaaacacc attgaacaat ccgccattgc gactttgcaa ggcttgggct gggactacac
       61 ctacggcaaa acgattttgg caggtttaga acacgaatgg cgggagcgag ccactgaggt
      121 ggtattaaag ccgctgcttg ccgaagcgat tgcaaaattc aacccgaatt tgcccgcttg
      181 tgaggtggaa aatgtggtgg cacaggtgtg ccgtgcggaa agtagtgatt tggcggagcg
      241 taatcgtcag gcttatgatt ggctaagaaa tggggtcaaa atcacttatc agttggacgg
      301 tgagcaggtg tctgatgtgg tgcagctgat tgattttcag cgtccagaaa ataacgattt
      361 tcgcattgtc aatcagctgg atattggcgg taaaaaaggc aaacgcattc ctgatttgat
      421 tggctttgtc aatggcttgt cgctggtggt atttgagctg aaaaatccgc tcaaagaaaa
      481 tgccgacatc ggcaaggcgt ttgcccaact gcaaacctat aaagatgaaa tttctgattt
      541 gtttgtgttt aatcaggcac tcgtgatttc agacagcatt gtcgcccgta tcggttcgct
      601 gaccgccgat ttcgaccgtt ttaccccttg gcgtgtggtt gatgaaaaaa atcagaacaa
      661 acgcattgtg tttgaagatg aactcaccgc cctactgcaa ggcgtgatga accccaaaaa
      721 tctactggat tatgtgcaga attttgtggt gtttgaacgg gacggcaaaa accgtttgat
      781 taagaaaatc ggggcgtatc atcagtttta tggcgtgaat gaagcggtgg attgcacctt
      841 gcttgccacc acaggcaacc gcaaaatcgg tgtgttttgg catacgcaag gctcgggcaa
      901 atcgctttcg atgctgtttt atgcaggtaa agtattaagc caaggctgcc tgaaaaatcc
      961 aactttggtg ttagtcaccg aacgcaacga tttagacggt cagctttacg ccactttttg
     1021 cggtggtgag gcattgctca aacaaacgcc tatccaagcc gatgggcgag acgaactccg
     1081 ctctgccctt gccagtcgtt cagcaggcgg tgtaattttt accaccattc aaaaatttgg
     1141 cttaatggag ggcgagcttg cccacccagt gttaaacgag cgggaaaaca tcattgtgat
     1201 taccgatgaa gcacaccgtt cgcaatatgg ttttagccaa aaaatcaacc acaaaggcga
     1261 gtatcgtgag ggttacgcca agcatttacg cagtgcattg ccgaatgctt cttttattgg
     1321 ttttacaggc acgccgattg aacttgatga caaagacacc caagaagtct tcggtaaata
     1381 tgtctcgatt tatgattttg aagatgcggt ggaagatggg gcaaccgtgc cgattattta
     1441 tgagccacgc caaatcagct taggcgaaag tggcgagttt accaaagtga tggaagaggc
     1501 acaacaactg attgatgacg atgaaaacag ctataacttc cgcctgcgtg aaaaactgca
     1561 cagcgtggat agccgtttgc aaaaaatggc ggaagatatt attgcccatt acgatgagcg
     1621 taccaaacag caagacggca aggcgatggt tgtggtgatg agccgtgcca tttgcgtgaa
     1681 attgtacgaa aaaatcaccg cacttcgtcc agaatggcac tcaaacgatg tgcttcaagg
     1741 cagcattaaa attgtgatga caagtaatgc cagcgaccct gccgagtggc aaaaacacaa
     1801 tcaggataag aaaaccttag aaaaacgctt taaagaccca gacgatccgc tcaacatcgt
     1861 gattgtgcgg gatatgtggc tgacaggctt tgatgcgccc tgctgtaaca caatgtacat
     1921 tgataaacca atgagcggac acaacctgat gcaggcaatc gctcgggtaa atcgggtatt
     1981 tcgcaacaaa agccgtgaaa atggcggctt gattgtggat tatgtgggct tgaccgatga
     2041 gctggaaaaa gcgatgaagc agtacaccaa cgcaggcggc aaagaaaagc cggtgcggga
     2101 catttcagcg gtgctggaaa aaatggtgga acatatcacg gtcattcgtg ggcaatttgc
     2161 cacaccgatt gacggacaag cggttgatat tgccaaaatg ttgcaaatca gcgaaccgcc
     2221 taaactgctc aatgcgattt tgacatcagc caaccatatt cttgccctag accgcattca
     2281 gccacctgat aacactgcca aagacaaaac cccacgcaaa aacgcctttt tgcaatcggt
     2341 acgcttggcg aaaaaaggtt atgctttgtg tggggcatta aaagcagttg aaccctataa
     2401 acaggaactg gcgttttatg atgcagtgcg tgccaccatt atcaaaaaca gcaccgctcc
     2461 tcgcaattct tcgagcgaaa atgaccgctt gttgcagctt accgccttga tgaatcgtgc
     2521 ggtacagtcg gatggtgtgg tggatttatt tgatttgctg aaaaaagacc gcccaaacat
     2581 caacctgctt tctgatgagt ttttggaaac agtaaaaaac agcccaacca aagatttgtg
     2641 gctatcagca atggagcgtt atcttgcctc acaactgcga gagcaaagcg gtgccaatct
     2701 tgccacaaaa aaagcatttg aacagaaact caaagaggcg atgaaccaat accacaacca
     2761 caatttaagc gtattggaaa ttttagaaga gctgatcgcc ctcgccaaag agtttgaagc
     2821 ccgccaaaaa cgaggggaag cgttgggact aagccctgcg gaaatggcgt tttatgatgc
     2881 tttggcacgt aatgaaagtg cggtgcggga aatgggcgat gaggtattga tgaaacttgc
     2941 caaagacatt accgataaat tacgcaaatc ggtcaccgta gattggcaat ataaagactc
     3001 ggttcgtgcc aaaatgcgaa ccttaatccg cattgctctg cgtacctata aatacccgcc
     3061 cgatttacag gcggaagcga ttgagtttgt gttgcaacaa gcggaagaga tcgccggaga
     3121 gttcgctatc accgaaacgg cgtaatctat attagggcgt gcaatgtacg ccctaaattt
     3181 atgctaataa cctctgccac atttcccgca caaatgctcg ctcggtggta aattcacttg
     3241 cgtccaccac gctgtctttg cgtaataagt ttaggtggtg gattttgtta cgcagtgcgg
     3301 tgtagcaatg ttgtagctgt tcgctttgtt cggaacttaa aataccgcat tctactgcgc
     3361 tgtcgaaaat tcggacatta tccgaccata ctgccatttt cgggtgttgg tggctgtggg
     3421 ctaataccaa atattgggcg ataaattcaa tatcggtaat acctccttga tcttgtttaa
     3481 tatgaaattg ctcggctgaa tgcgagctta aatgttggta cattttctgt cgcatttcgc
     3541 aaatttcttg gcgtaattga ccgcttgcac gaggctgagc aagcgtgctt tggcggattt
     3601 tttcaaaggc ttgtttgagg ttttccgccc caaatacaca tcttgtccgc acgagggctt
     3661 ggctttccca cgtccacgct tcattttttt gataatgctc gtaggcattg aaagtgctga
     3721 ctaaaagccc agcctcgcct gatgggcgaa gtcgcatatc cacttcatat agtacgccgg
     3781 cactggtatt aaggttaaaa atcgagttaa ttttttgagc caattttaag taaaattgat
     3841 ggcttgaaat ctctttttta ccgccaacag tttggctatc ctctggggca ttatgcaaga
     3901 aaaccaagtc gagatctgaa ttatagccca gttcaatccc gcctagcttg ccgtagccaa
     3961 taactgcaaa ccctttttca tcagcctcta aatgttccgg cacaccaaag cgttggctga
     4021 cttgcttcca cgccatattt accaccgcat taataatcgc ttctgctaag taagtaagat
     4081 gatcgctgat cttcattacc agcagcacgc ctaaaatatc agcagcggcg atgcgtaaaa
     4141 tttgggtttg tttgaactga cgtaagccat caatcagtgc ttcttcatct tcttcaggaa
     4201 tgcggattaa ataatcctgc aaggcagatt gatatttttc aaaagcctgc acgtcggtta
     4261 agcctctatg gctcatcagc tcatcaagta gcattggata tcgagctatc tgttccgcaa
     4321 tcataaccga cttactacac aatgctaaaa gttgaggcag aatctgctct ttttcgagca
     4381 ttaattccaa ataggtagtt cgggtggtga ttttatcgac aatattcaac acgcgaggca
     4441 ataacaataa atgatcttca cctttaaaaa tgctgtcggc aattctaggc attaaattgc
     4501 gtagcacctc tcgccctctt accccaatcg ggcgttttac ccaatcttga aaggtagtag
     4561 aaaagtgacg gaaaatctcc gtataatcat tttctgcaac ctttggataa ccttttagca
     4621 ctgcggataa atcttcaagg cgaatgttat agtgcaaaat gtctttccat tcggctaatt
     4681 tttcattaac cggattgcag ttggcttgtg tttcctcttc accgataatt tcattaaaaa
     4741 tctggcgtac tgcttgttga tattggctca atactgccat aaattgttgc caatcgttaa
     4801 aggaatgctc cacccaatgc gttttttcgc tatatagatc ttgctgataa tagctttggc
     4861 aagcaaatac aaggcgggct tgcgaggctt catcagtggg tagggtttga gtttgtttat
     4921 cgtcaatcgc ttgtaataca ttttcaattt gacggtaaaa aatataggca ttgtggagcg
     4981 tttccacctg cgtttgggtt aataaatt
//
LOCUS       DANKBV010000150         4913 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.150, whole genome shotgun sequence.
ACCESSION   DANKBV010000150 DANKBV010000000
VERSION     DANKBV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.150"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1369)
                     /gene="thiE"
                     /locus_tag="PWY43_002168"
     CDS             complement(<1..1369)
                     /gene="thiE"
                     /locus_tag="PWY43_002168"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDL2137015.1"
                     /translation="MSNYSNSPYQPNVIWTVAGSDSCAGAGLQTDLHTFHDFNLVGCS
                     VVTSVTAQHPHGVLCVTPVDDHTFRQQFEALLVQGYPNAIKIGLLCSQAQVEILCEYI
                     QKIRAESSHYCYVVYDPVAVASSGQALSDSILLPIVQQKLYPLVDLITPNGTELALLS
                     DTEIQTFGDVKTASHKLFAQGIKAVLAKGGHFEWLGETVRDYLLTANEIYAFSHARQQ
                     SVNTHGTGCTYASAVGAMLATGFDLADAVTVATAYLQKGLSEKQGRGQTALSSLCHTG
                     YPDDIAFFPQTELISAPLKLPKEPFAPTEYQLGLYPVVDSVKWLERLILVGVKTLQIR
                     IKNRPLAEIEQEIAHSVALAKQHNVRLFVNDYWQLAMKYQAYGVHLGQEDLATADLNA
                     IQQSGLRLGVSTHCYFEIMRALAVKPSYIAFGHVFPTQTKVMPSQPQGLTNLAKYVAL
                     VAPLNI"
     regulatory      complement(1437..1533)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1669..2982)
                     /locus_tag="PWY43_002169"
     CDS             complement(1669..2982)
                     /locus_tag="PWY43_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2137016.1"
                     /translation="MSKTHSNPTKVAVASMVGTAIEYFDNYIYTMAAVLVFNVQFFNS
                     DDPVSNQLMSLSVLALAFFTRPMGSILFGHFGDKYGRKQTLIASLLLMGLSTVAIGLL
                     PNYAAIGIWATVLLCLCRIGQGLGLGGEWGGAALVATEHAPKHKRAFFGIFPQMGAPI
                     GLLLANGAFYLVNLIYGEQTFLDWAWRIPFVASIVMIAIGLYIRIKISESPIFLKAEQ
                     QGKNRHTPLKTLFSQHLKPFMIGVLIATAGYVLFYILVAFTPIFVKSPVAVSQAGHAT
                     GLGLPVNLYTQYLLITSVIFGIAIFFSGIYSDKIGRRLLLLLATGATVVYGLTMPFFL
                     ENGTPTSIFIFLTIGMILIGLSFGPMAVILPELFPTEVRYTGASLAYTAAGILGASVF
                     TIIAVKINENFGLFGVGGYLSVASLLSFWALWQVHETKDLELEEI"
     gene            complement(2975..3793)
                     /gene="thiM"
                     /locus_tag="PWY43_002170"
     CDS             complement(2975..3793)
                     /gene="thiM"
                     /locus_tag="PWY43_002170"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDL2137017.1"
                     /translation="MTTSLPYHSHYLQKIRQNRPLVHNITNIVAANFSANGLLALGAS
                     PIMAAAIEEMESVPALSNAVVINIGTLIGKEVEAMELAGKTANRLGIPLILDPVGVGA
                     TPFRRQTVETLVDQIHFTAIRGNAGELATLAGVEWQAKGVDAGEGSHNVVEIAQQIAK
                     RYQTLVAISGETDVVSSGSHTALLHNGSPMFPKITASGCLLSAVCGAFLAVADKEHYF
                     NALIEACAAYAVAGEVAAQALKPTQYGQFAVNLLDTLGALTPETVEQYVRVEYV"
     regulatory      complement(3850..3946)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4003..>4913)
                     /locus_tag="PWY43_002171"
     CDS             complement(4003..>4913)
                     /locus_tag="PWY43_002171"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611280.1"
                     /note="Catalyzes the reduction of nucleoside
                     5'-triphosphates to 2'-deoxynucleoside 5'-triphosphates;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2137018.1"
                     /translation="DNPDSLASCCRLRNEISDHSFSYSLGAGGVATGSINVITINMNR
                     LVQDNRDLSEEVSKIHKYQYAYRKLMEEYLAAGMLPVYDAGFISLHKQFLTIGINGMA
                     EAAESQGLTVGYNPDYIDFVQSRLKIIFQANQAAGEKYGVKFNTEFVPAENLGVKNAK
                     WDKEDGYFVPRDCYNSYFYVVEDEEINALDKFLLHGRELIEWLDGGSALHLNLDEALT
                     QTGYRSLLDTAAKTGCNYFCINVKITICNECSHIDKRTLHSCSKCGSDNIDYGTRVIG
                     YLKRVSAFSSARQKEHSLRFYHREAA"
BASE COUNT         1361 a         1098 c         1152 g         1302 t
ORIGIN      
        1 gaatgttaag cggtgcaacg agagcaacat atttggctaa atttgttaag ccttgtggtt
       61 gagacggcat tactttggtt tgagtaggga aaacgtggcc aaaagcgata taagacggtt
      121 tcaccgccaa tgcccgcatt atttcaaagt agcagtgggt tgaaacacca agccgtaacc
      181 cagattgctg aattgcattt aaatcggcgg tggctaaatc ctcctgccct aaatgcacgc
      241 cgtaagcctg atacttcatg gctaactgcc agtaatcatt tacaaacaaa cgcacattgt
      301 gctgtttcgc cagtgcgacc gaatgggcaa tttcttgctc aatctctgca agcggtcgat
      361 ttttgattct aatttgcaag gtttttaccc ctacaagaat taagcgttca agccatttaa
      421 cgctatctac aacagggtaa agcccaagtt gatattccgt tggagcaaaa ggctctttcg
      481 gcaatttaag aggggcagaa atcagctcgg tttgtgggaa gaaagcgata tcatcagggt
      541 agccggtgtg gcaaagtgaa cttaaagcag tttgccctct gccttgtttt tccgataagc
      601 ctttttgcaa ataagcggtg gcaacagtga cggcatcagc taaatcaaag ccggttgcca
      661 acatcgctcc aaccgctgaa gcataggtgc aacctgtgcc atgggtattc acactttgct
      721 gtcgggcgtg actaaaggca taaatttcat ttgcagtcag cagataatct cttaccgttt
      781 cccccagcca ttcaaaatgt ccacctttgg ctaacacggc tttgattccc tgtgcaaaca
      841 gtttgtggct agcggttttc acatcaccaa aggtctggat ttctgtgtcg cttaataaag
      901 cgagttcagt gccgtttgga gtgattaaat ccaccagcgg atacaatttt tgttgcacta
      961 tcggcaataa aatactgtcg gataacgcct gcccactgct tgccactgcc acagggtcgt
     1021 aaaccacata acaataatgc gagctttctg cacggatttt ttggatatat tcacacaaga
     1081 tctctacttg tgcttgcgag cagagtaagc cgattttgat tgcgttcggg tagccttgaa
     1141 ccagcaaggc ttcaaactgt tggcggaagg tgtgatcatc aacaggcgtt acacacagca
     1201 caccgtgcgg atgttgagcc gttacagaag taactactga acaacccact aggttaaaat
     1261 cgtgaaaggt atgtaaatcg gtttgcagac cggcaccggc acaggaatct gagccggcaa
     1321 ccgtccaaat gacgttaggt tggtatggag agtttgaata attagacatc gcaaaatcct
     1381 cataatatgc ttagggtttt gcttgccaga gatgtggtaa tgatcccaaa atctgaattt
     1441 gggtaaaact tgttccctac gcaggtacta acctacaggt tccacggatc tcgctgttgc
     1501 gatctcagcc ttacggcact ccgacaagcg tttttagtat agcttacaag cggttatttt
     1561 ttataaaaaa atgcaaaata tccgttgtga agtctattag agggaaattt gcattccctc
     1621 atcttggggc aaggtggtaa cttgccccgt cgttaagtga gtggtttttt atatctcctc
     1681 taattccaaa tctttggttt cgtggacttg ccacaatgcc cagaagctca gtaatgaggc
     1741 aaccgataaa tagccgccta caccgaataa gccgaaattt tcattgattt tgaccgcaat
     1801 aatagtgaac acgctagctc ctaaaatacc ggcagcggtg taggcaagcg aagcccctgt
     1861 gtaacgtact tctgttggga agagttcggg tagaattact gccatcgggc cgaagcttaa
     1921 gccgattaag atcattccaa tggtgaggaa aataaagatc gaagtcggtg tgccgttttc
     1981 gaggaaaaac ggcattgtta agccgtaaac tacggttgct cctgtggcaa gtaacagcag
     2041 taaacggcgt ccgattttgt ctgaataaat gccggagaag aaaatcgcaa tgccgaaaat
     2101 cacggaggta atcagcaaat attgggtgta gagatttacc ggtaaaccta agcctgtagc
     2161 gtgtcctgct tgagaaactg ccaccgggct tttgacgaaa atcggggtga aagcaactaa
     2221 aatgtagaac aacacatagc cggcggtggc aatcagcacg ccgatcataa aaggcttgag
     2281 gtgttggcta aacaaagttt taagcggggt atggcggttt ttaccttgtt gttccgcttt
     2341 aaggaaaatc gggctttcgc tgatcttaat tcggatataa agcccaatgg cgatcatcac
     2401 aatagaagcg acaaacggaa ttcgccacgc ccaatccaag aaggtttgtt caccgtaaat
     2461 taaattcacc aagtagaacg ctccgtttgc caacagcaag ccgattgggg cgcccatttg
     2521 cggaaaaata ccaaaaaatg cccgcttgtg tttcggggcg tgttcagtgg caacgagagc
     2581 agctccgccc cattcgccgc ctaagcctaa accttgtccg attctacaga ggcagagtaa
     2641 cacggttgcc caaatgccaa tggcggcata attaggcaaa agcccgattg ctacggtaga
     2701 taagcccatt aataagagcg aggcaatcag agtttgtttg cgtccgtatt tatcgccgaa
     2761 atgcccgaat aaaatcgacc ccatcgggcg ggtgaaaaag gcaagagcta gtacggaaag
     2821 cgacattaat tggttggaaa ccggatcatc gctattaaaa aactgcacat taaacaccag
     2881 cactgctgcc atcgtgtaga tgtagttatc aaaatattca atcgccgtgc cgaccattga
     2941 ggcaaccgcc actttagttg ggtttgagtg agttttagac atattcaacc ctcacatatt
     3001 gctcaacggt ttcaggcgtt aatgccccaa gggtatccag cagattgacg gcaaattgcc
     3061 cgtattgagt tggtttgagt gcttgagcag ccacttcgcc tgcaacggca taagcggcgc
     3121 aagcctcaat aagtgcgttg aaatagtgct ctttgtcggc aacggctaag aatgccccgc
     3181 ataccgcact taataaacag cctgaagcgg taattttggg gaacatcggg ctgccgttgt
     3241 gcaataaagc tgtgtgtgag ccagagctta ccacatcggt ttcaccactg attgcgacta
     3301 aggtttggta acgtttggca atctgttggg cgatttcaac cacattatgc gagccttcgc
     3361 cggcatcaac gcctttggct tgccattcta caccggcgag ggtagctagt tcccccgcat
     3421 ttccacgaat ggcggtgaag tgaatttggt cgactaaggt ttcaactgtt tgacggcgga
     3481 aaggtgttgc tccaacgccg acagggtcaa gaatgagcgg aatacctaaa cggttggcgg
     3541 ttttgccagc aagctccatt gcttctactt ctttgccgat aagcgtgccg atattaatca
     3601 ccacggcgtt actgagtgcc ggcacggatt ccatctcctc aatagcagct gccataatgg
     3661 gagaagcacc aagagccaat aagccgttgg cagaaaaatt agcggcaaca atgttggtaa
     3721 tgttgtgaac aagcggtcga ttttgtcgaa ttttttgcaa ataatgcgag tgatagggga
     3781 gtgatgtggt catcgtttat tccttaatat gattgaaaaa caacgcacca acaccaacgg
     3841 ggaatagatg ggtttacgcc tttagtttcc tacgtcagtg ctaactgcat caggttcaac
     3901 gggtatttct cagcttgccc ttaggcaagc accccgacta aatgaggctt taatataatc
     3961 actaaaaaag cggttgtaaa gggctacaac tgcagttttc aattaagctg cttctcggtg
     4021 atagaaacgc aagctgtgct ctttttggcg ggcggaacta aaggcggaga ctcgttttaa
     4081 atagccgatt acccgagtgc cgtaatcaat attatcggag ccacatttgc tacaactgtg
     4141 caacgtgcgt ttgtcgatgt ggctacattc gttgcaaatc gtgattttaa cgttaataca
     4201 aaaataattg cagccggttt tagcggcggt atccagcaag gagcgatatc cggtttgggt
     4261 taaggcttcg tctaaattca agtgtagagc ggagcctccg tccagccatt caatcagctc
     4321 tctgccgtgc agtaagaatt tatccaaagc attaatttct tcatcttcta ccacatagaa
     4381 ataggaattg tagcaatctc gtggtacgaa atagccgtcc tctttgtccc atttggcgtt
     4441 tttgacccct aaattttcag caggcacaaa ttcggtatta aattttaccc catacttttc
     4501 accggctgct tggttagctt ggaaaataat ttttaaccga gattgcacaa aatcaatata
     4561 gtctggattg taacctacgg ttaaaccttg tgattcagca gcttctgcca ttccgttaat
     4621 gccaatagtt aggaattgtt tatgcagtga aataaaaccg gcatcgtaaa ccggcaacat
     4681 accggcagct aaatactctt ccatcagttt gcggtaagcg tattgatatt tatggatctt
     4741 gctgacttct tcgcttaaat cacggttatc ttgcaccaaa cggttcatat taatagtaat
     4801 tacattgatt gaacctgtcg caacacctcc agcccctaat gaataggaga agctatgatc
     4861 gctgatttca tttctcaagc ggcagcaaga agcaagggaa tccggattat ctg
//
LOCUS       DANKBV010000151         4871 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.151, whole genome shotgun sequence.
ACCESSION   DANKBV010000151 DANKBV010000000
VERSION     DANKBV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.151"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            253..714
                     /gene="rimP"
                     /locus_tag="PWY43_002172"
     CDS             253..714
                     /gene="rimP"
                     /locus_tag="PWY43_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL2137019.1"
                     /translation="MATLEQKLEDLVLDSIEALGFELVGIECQRAGRFLTVRLYIDKE
                     GGVTIDDCSDVSRQVSAIFDVEDPIADKYNLEVSSPGLDRPLFTVAHYERFIGQDVVI
                     HLRIPMFDRRKWQGKIKQVEGDFITLTVENEDCQFAFGNIQKANLVPVFNF"
     gene            735..2231
                     /gene="nusA"
                     /locus_tag="PWY43_002173"
     CDS             735..2231
                     /gene="nusA"
                     /locus_tag="PWY43_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL2137020.1"
                     /translation="MSKEILLAAEAVSNEKQLPKEAIFEALETALTIATKKSRDLDID
                     VRVVVDRKTGDFQTFRRWAVVENVNNMTREISLEAAQFEDPNVQLGDFIEDEVESIEF
                     GRVAIQTFGQVVKTKIREAEKTKIIDEFRSDLGKIILATVKKVTRDNIFLELQGKSED
                     IKGEAVIVREDMIPRENFRPGDRVRGVLYEIKPESKGPQLFVTRAKPVMLEELFKLEV
                     PEIGEGVIEIKGSARDVGSRAKIAVKTHDKRIDPVGACVGMRGARVQAISNELGGERV
                     DIVLWDENPAQFVINAMAPADVSSIVINDETHSMDIAVEAKNLAQAIGRNGQNVRLAT
                     QLTGWALNVMTVEELTKKSEEEDGKVINLLTSALEIDEEFAQVLIEEGFTSLEAIAYV
                     PVDTLTAIDGLEDEDLVEELQSRAKNAITKKALEEEKLLKSAQIEEKLLTLEGMDRHI
                     AFKLAEKQITTLEELAEQGVDDLADIEELSEEKAGELIMAARQICWFN"
     gene            2216..4783
                     /gene="infB"
                     /locus_tag="PWY43_002174"
     CDS             2216..4783
                     /gene="infB"
                     /locus_tag="PWY43_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2137021.1"
                     /translation="MLVQLSKEDNKMTEEKKTLSLGGARKASKVNTTTTSGKVKAVEV
                     KEKKPKIDAKALQEKAAKEAAAAKARAEKEAAEKLAKEKAEAEAKEKAAKKAAESTAS
                     KPISVPVMPSSKAKEEKPKAQQVQPKQEKAVDPEKEAKRKEEAELRRKQEELAQQKAE
                     MEAKRAAENARRLAEIAREEAEDNGNEDFDDERFTSSYAREAERDNDRRSEGNRGRNG
                     KGNVAKAKKGGREEDKNERSADRRNHKEVKGKGKQGKKGAALQQAFTKPAQVVKADVV
                     IGETITVAELANKMAVKATEIIKMMMKMGEMVTINQVIDQETAQLVAEELGHKVILRK
                     ENELEEAVLEDRDTHAEKVTRAPVVTIMGHVDHGKTSLLDYIRKAKVAAGEAGGITQH
                     IGAYHVETDDGKMITFLDTPGHAAFTSMRARGAKATDIVVLVVAADDGVMPQTIEAIQ
                     HAKAAGAPLVVAVNKIDKPEANPDRVEQELLQHEVISEKFGGDVQFVPVSAKKGMGID
                     DLLEAIILQSEVLELTAVKDGMASGVVIESYLDKGRGPVATILVQSGTLNKGDIVLCG
                     FEYGRVRAMRDENGKDVESAGPSIPVEVLGLSGVPAAGDEATVVRDEKKAREVALYRQ
                     GKFREVKLARQQKAKLENMFSNMAAGDVAELNIIVKADVQGSVEAISQSLMELSTDEV
                     KVKIVGSGVGGITETDATLAAASNAIMVGFNVRADASARRVIENENIDLRYYSIIYEL
                     LNDVKAAMSGMLQPEFKQEIIGLAEVRDVFRHPKFGAIAGCMVTEGIVKRNNPIRVLR
                     DNVVIFEGELESLRRFKDDVSEVRNGMECGIGVKNYNDVKVGDQIEVFEVVEVKRTI"
BASE COUNT         1565 a          863 c         1226 g         1217 t
ORIGIN      
        1 tggttcaaat ccggcccccg caaccagttt taagctcaca gctttggtta aagttgtgag
       61 ttttgtttta tatgctgtag caaatttgca aaaaatttgg caaaaaagac cgcttgttgt
      121 ggttgctaca gcaaaaaaca aaccgcttaa agccccacgg tttcccgtct ggggcttttt
      181 tgcggaaatt ggtacagcat taacgtatca aagctggttt atccggcttt ttttatattt
      241 ggagattatg acttggcaac tttagaacaa aaattagaag atttagtgct cgattctatt
      301 gaagcattag gctttgaatt agtcggcatt gaatgccaac gtgcaggccg ttttttaacg
      361 gtgcgtttat atattgataa agaaggtggc gtgacgattg atgattgtag cgatgtcagc
      421 cgccaagtga gtgcgatttt tgatgtggaa gatccgattg ccgataaata taacttagag
      481 gtttcttcac cagggttaga tcgtccatta tttaccgttg cccactatga gcgttttatc
      541 gggcaagatg tagtgattca tttacgcatt ccgatgttcg atcgccgtaa atggcaaggt
      601 aaaatcaaac aagtagaagg tgatttcatc actttaacgg tagaaaatga agattgccag
      661 ttcgcatttg gcaatattca aaaagcaaat ttagttccgg tttttaattt ttaagataaa
      721 ggtaaactga caaaatgagt aaagagattt tattagctgc ggaagcagtt tcaaacgaaa
      781 agcaattacc aaaagaggca atttttgaag ccttagaaac cgctttaacc attgcaacca
      841 aaaagagccg tgatttagat attgacgtgc gtgtggtggt agatcgtaaa accggtgatt
      901 tccaaacttt ccgtcgttgg gctgtggttg aaaatgtgaa taatatgacc cgtgaaatca
      961 gcttagaagc ggcacaattt gaagatccaa acgtacaatt aggcgatttt attgaagatg
     1021 aagtagagtc tatcgagttc ggtcgtgtgg cgattcagac ttttggtcag gtagtcaaaa
     1081 ccaaaattcg tgaagcggag aagacgaaaa tcattgatga attccgttcg gatttaggca
     1141 aaattattct tgccactgtg aaaaaagtaa cccgtgataa tattttcctt gagttacaag
     1201 gtaaatcaga agatatcaaa ggcgaagcgg tcattgtgcg tgaagatatg atcccacgtg
     1261 aaaacttccg cccgggtgac cgtgtgcgtg gtgtgttata cgaaatcaag cctgaatcaa
     1321 aaggacctca gcttttcgtt acccgtgcta aaccggtgat gttagaagag ctattcaaat
     1381 tggaagtgcc tgaaattggc gaaggcgtaa ttgagatcaa aggttcagcc cgtgatgtcg
     1441 gctctcgtgc aaaaattgcc gtgaaaaccc acgataaacg tattgacccg gtaggggctt
     1501 gtgtgggtat gcgtggtgct cgtgttcaag ctatcagcaa tgaattaggc ggcgagcgtg
     1561 tggatatcgt actttgggac gaaaatccgg ctcaatttgt gattaatgca atggctccgg
     1621 cagatgtaag ctctattgtg attaacgatg aaactcactc aatggatatt gcggttgaag
     1681 cgaaaaatct ggctcaagct atcggtcgta acggtcaaaa cgtgcgtttg gcgacacaat
     1741 taactggttg ggcattaaat gtaatgaccg ttgaagagct aacgaagaag agcgaagaag
     1801 aagatggtaa agtcatcaat ttattaactt ctgctttaga aattgatgaa gaatttgctc
     1861 aagttttaat tgaagagggc tttacgtctc ttgaagcgat tgcttatgtg ccggttgata
     1921 ctttaacggc gattgacggt ttagaagatg aagatttagt cgaagaatta caatctcgtg
     1981 ccaaaaatgc gatcacgaaa aaagcgttag aagaagagaa gttattaaaa tcagctcaaa
     2041 ttgaagaaaa attattaacg cttgaaggta tggatcgcca tatcgcattc aaattagctg
     2101 aaaaacagat tacgactctt gaagaacttg cagagcaagg tgttgatgat ttagccgata
     2161 ttgaagaact ttcagaagag aaagctggcg agctaattat ggcagcacgc caaatttgtt
     2221 ggttcaacta agcaaagagg ataataaaat gacagaagag aaaaaaacac tttcgcttgg
     2281 tggcgctcgc aaagcgagta aagtgaatac aaccacaacc agtggtaaag taaaagcggt
     2341 tgaagtaaaa gagaaaaaac cgaaaattga tgcaaaagca ttgcaagaaa aagcagcaaa
     2401 agaagctgcc gcagcgaaag ctagagctga aaaagaagca gctgaaaagt tagccaaaga
     2461 aaaagccgaa gctgaagcta aagagaaagc tgcaaaaaaa gcagcagaat cgaccgctag
     2521 taaaccgatt tctgtgccgg taatgccaag cagcaaagca aaagaagaaa aaccgaaagc
     2581 acaacaggta cagccaaaac aagagaaagc ggttgatcct gaaaaagaag ctaaacgcaa
     2641 agaagaagct gagttacgcc gtaagcaaga ggaattagct cagcaaaaag cggaaatgga
     2701 agcaaaacgt gctgcagaaa atgcccgccg tttagcagaa attgctcgtg aagaagcgga
     2761 agataacggc aacgaagatt ttgatgatga gcgttttact tcttcttatg ctagagaggc
     2821 tgagcgtgat aatgaccgcc gtagtgaggg aaatcgtggt cgtaacggca aaggcaatgt
     2881 ggctaaagcg aaaaaaggcg gacgtgaaga ggataaaaac gaacgcagtg cagatcgtcg
     2941 caaccataaa gaagtaaaag gcaaaggtaa acaaggtaag aaaggtgcag ctttacaaca
     3001 agcctttacc aaaccagctc aagttgttaa agctgatgtg gtgattggtg aaactatcac
     3061 tgtggctgaa ttggctaata aaatggcagt gaaagcgact gaaatcatca aaatgatgat
     3121 gaaaatgggt gaaatggtga cgatcaacca agttatcgac caagaaaccg cacaattagt
     3181 ggcggaagaa cttgggcata aagtgatttt gcgtaaagaa aatgagttag aagaagcggt
     3241 tttagaagat cgtgatactc acgcagaaaa agtaacccgt gcaccggtag tgactattat
     3301 gggccacgtt gaccacggta aaacctcgtt acttgactac attcgtaaag ccaaagtggc
     3361 ggcaggtgaa gcaggtggta ttacccagca cattggtgca taccacgttg aaaccgatga
     3421 cggcaagatg atcactttct tagatacccc ggggcatgca gcctttacct caatgcgtgc
     3481 gcgtggtgcg aaagcaactg acatcgttgt tctcgtcgtt gcggctgatg acggtgtaat
     3541 gccacaaact attgaagcta tccagcacgc caaagcagcg ggtgcgccat tagtggtagc
     3601 ggtgaataaa attgataaac cggaagcgaa ccctgaccgt gtagaacaag agttactaca
     3661 acacgaggtt atttctgaga aattcggtgg cgatgtgcaa ttcgtgccgg tatctgcgaa
     3721 aaaaggtatg gggatcgatg atttactaga agccattatt ctgcaatccg aagtgcttga
     3781 attaaccgca gtaaaagacg gtatggcaag cggcgtggta atcgaatctt acttagataa
     3841 aggtcgtggc cctgtggcaa ctatcttggt tcaatccggt acgctaaaca aaggcgacat
     3901 tgttctatgt ggttttgaat acggacgtgt gcgtgcaatg cgtgatgaaa acggtaaaga
     3961 tgttgaatca gcaggtccgt ctattccggt agaagtatta ggcttatcag gcgtgcctgc
     4021 tgccggtgat gaggcaaccg tcgtgcgtga tgagaagaaa gcccgagaag tggctcttta
     4081 tcgccaaggt aaattccgtg aagtaaaact ggctcgtcag caaaaagcta aactggaaaa
     4141 tatgttcagt aatatggcag caggtgatgt ggcagaattg aacatcattg ttaaagcaga
     4201 cgtacaaggc tcggtagaag cgatcagcca atccttaatg gaactttcaa ccgatgaggt
     4261 gaaagtgaaa attgttggtt caggtgtagg tggtattacc gaaactgatg caaccttagc
     4321 cgcagcttct aatgctatta tggtcggctt taacgtgcgt gccgatgcct ctgcccgccg
     4381 tgtgattgaa aacgaaaata tcgatttacg ttactactcc atcatttatg agctattaaa
     4441 tgacgtgaaa gcagcaatga gcggtatgtt acagcctgaa ttcaaacaag aaattatcgg
     4501 cttggcagaa gtgcgcgatg tgttccgcca cccgaaattt ggggcgattg ccggttgtat
     4561 ggtgacagaa ggtatcgtta aacgtaacaa cccaatccgt gttctgcgtg ataacgtagt
     4621 aatttttgaa ggcgagttag aatctctccg ccgtttcaaa gatgacgtat ctgaggtgcg
     4681 taacggtatg gaatgtggta tcggcgttaa aaactacaac gacgtaaaag ttggcgacca
     4741 aatcgaagtg tttgaagtgg ttgaagtaaa acgtactatt tagcctaatt ttgtgtaggg
     4801 gcgtattgca tacgcccttt gtatttttta gggcgtgaag catcacgccc ctataaaaga
     4861 gaaaaatatg t
//
LOCUS       DANKBV010000152         4848 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.152, whole genome shotgun sequence.
ACCESSION   DANKBV010000152 DANKBV010000000
VERSION     DANKBV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.152"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..280)
                     /locus_tag="PWY43_002175"
     CDS             complement(<1..280)
                     /locus_tag="PWY43_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2137022.1"
                     /translation="MALNVPLHRYFDYLLPPAFNACQGGRVVVPFGRQTKIGIVVDFP
                     QTSDVPTEKLKPIKAALDSSPIFDSEMWQLLNWAARYYHAPIGEVLTGA"
     gene            complement(292..904)
                     /locus_tag="PWY43_002176"
                     /pseudo
     CDS             complement(292..904)
                     /locus_tag="PWY43_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(897..1794)
                     /gene="rarD"
                     /locus_tag="PWY43_002177"
                     /pseudo
     CDS             complement(897..1794)
                     /gene="rarD"
                     /locus_tag="PWY43_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
     gene            complement(2021..3238)
                     /locus_tag="PWY43_002178"
     CDS             complement(2021..3238)
                     /locus_tag="PWY43_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2137023.1"
                     /translation="MDRFPKSDKLAQVRYDIRGPIHKEALRLEEEGHKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIVQYYQSKGMRGMDVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPMPDYPLWTAASTLAGGKAVHYLCDEENEWFPDLED
                     IKSKITPRTKGILVINPNNPTGAVYSRQILLEIAELARQHNLIIFADEIYEKIVYDGA
                     VHHHIAALAPDVLTVTYNGLSKAYRVAGFRQGWMVLSGPKHQAKGFIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFVLPGGRLLEQRNKMHELLVQIPGISCVKAKGALYM
                     FPKIDTEMYGIKDDQKFIYDLLQQEKVLLVQGSGFNWKKPDHFRVVTLPYAHQIEEAI
                     GRLANFLKTYRQD"
     gene            3426..4730
                     /locus_tag="PWY43_002179"
     CDS             3426..4730
                     /locus_tag="PWY43_002179"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HDL2137024.1"
                     /translation="MLIFHQLKQELTKSYSKVGREQGLVAIQASMPLLEANIPLLGWL
                     KTQKTSYPHFFLQYRDTDQTIATIGTLRQFYTLEAAQSFIQQHHFPLIGGLQFEGNTQ
                     FILPQFSLVKNQQNLTACFYFDSDNFEQQAVIFEQFLANFSQITELGLTDNPLVSMSA
                     TSHFEQWKANIEKAIISIEQGDFQKVVLANAMRLHFEQPICAYSLLAQSQKTNLGCYH
                     FLWAESADVAFLGSTPERLYQRQGLYFSTEALAGTAAVTTSEVQTELNAQWLLSDPKN
                     IYENQLVVDDIENQLKDCVTEFAVSEAKIKRLHNVQHLRRQIQATLKPDTSDSDCLSK
                     IHPTAAVAGLPRQKAKGFITENEPFTRHWYAGTLGVMGNEEAEFCVTLRSALISQNSI
                     TLYAGAGIVAGSDPQSEWQEIKRKAQGMAKLLDVDLVTKGEL"
BASE COUNT         1568 a         1046 c          907 g         1327 t
ORIGIN      
        1 aggctccggt tagcacttca ccaatcgggg cgtggtaata acgagccgcc cagttcagca
       61 gttgccacat ttctgagtca aaaatcgggc tagaatccaa cgctgcttta atcggtttga
      121 gtttttcggt tggcacatca ctagtttgcg ggaaatcgac cacaatgccg attttggtct
      181 ggcgaccaaa aggcacaacc acccgcccgc cttggcaggc attgaatgcc ggaggtagca
      241 aataatcaaa ataacgatgt aacggcacat tcagtgccac tctgattaat ctcacttctc
      301 taaaccaatt ttgattgcaa gcccgataaa tatcgagccg cagattttat ttaaccaatt
      361 tccaaaggtt tgattatgcc gtaaaaaacg cccaacaggt gcagcggcta agactaaaat
      421 acttacccaa ataatgctgg tggataaaaa cgttagcccc aacagcataa aaggtatttt
      481 ccaaattact ggatacaaac tgcggcaata acgccaagaa aaagagcaat actttcgggt
      541 ttaatagatt ggtaatcaac ccttgacaga atatcttcca tagaggaagt ttgtctagat
      601 tgattttctc taacaccaac ggctctcgcc acattttaat gccgagatag cagagataaa
      661 ttgcccctgc gtatttcaga aaattaaata aagtgggcga atgtgccaca atgcctgcaa
      721 gcccgagtga aaccgcaaga atatggcata aaatgcctgc tagaatgccg aatgaagaca
      781 ttaagcccga ctttttcccc tcagcaatag tgcgagttaa aatatagata gtatccgaac
      841 ccggtgtaag atttagcaga atacaagcgg tcaaaaatcc ccaataattt acaatatcac
      901 tcattattca cactcaattt acgtttacgc tgctgacgaa gagcaaacat accatctaat
      961 actaaaaatg aaatggagat caacaagcaa atcatcaaca agtaagattc atttgctaac
     1021 ctttcgccaa ttaagaaaga caccaccaac atcaatagtg gctcaacata gcctaataaa
     1081 ccaagcaaat taaagggtaa aatcgtgctg gctagcgtat agctgataag tgcagtgcca
     1141 ctaattaaac ccagtaatgc aataaaatag taaatattgg ggttttgtgt ttcgacatat
     1201 tgcatatcgg tttgagagat aaaataacaa gctacaggga gcaaatataa aatttctacc
     1261 acaaagctat gtaaatggct taagccaaac tttcggcgaa ggtaaaaata gattgggtaa
     1321 cctgtgcaaa caaataaact tgctatcgaa atttttcccg taaacaaaat attactcgct
     1381 acaccaatga aagcaaccac aatcgccaac catttattca aggaaagcgt ttctttataa
     1441 accactttgc caaatgccac cataataatc ggcattaata gatagcccat tgaaacctca
     1501 atcgcttttc cgctattggg agcccataaa aacccacatc tgcccgccca caatagatgc
     1561 cgtgattaaa ataaccagca ataaataagg ctcttgcttt aggcgttgaa gaaataccca
     1621 aaactcattt tgttttttaa ataagtataa agcaaggaaa agaaaaggaa tcgtaaccag
     1681 catacgaatg ccgaaaatgc tcacacctgc cagcggtttt agaaagatcg ccaaatagta
     1741 catacagccg aataacactg aggctgaaag agataaaaga atgcctttta acatagtgat
     1801 aagaaagtaa ttatttttta atttttcaaa tctcccctac ccctcttttc taaagaggat
     1861 tgtcgagttc taaataaatg aataatgttc ggaggtaggg gcgggtccag tgcccgcccg
     1921 caaattaata tcgttagcgg acaggcacag gacctgtccc tactgatatt atagaactta
     1981 caaccctgct tttataaagt ggggtaaagg gagattcgac ttagtcttga cgataggttt
     2041 tcaagaaatt cgcaaggcga ccaatcgctt cttcaatttg atgtgcataa ggcaaagtta
     2101 ccacacggaa gtgatccggt tttttccagt taaaaccgga accttgcact aatagcactt
     2161 tttcttgttg caacaaatcg tagatgaatt tttggtcatc tttaatgccg tacatttcgg
     2221 tatcaatttt cgggaacata tataacgccc ctttcgcttt tacacagcta atacccggaa
     2281 tttgtaccaa taattcgtgc attttatttc gttgttccag caaacgaccg cccggcaaca
     2341 caaattcatt gatactttga taaccaccta atgcggtttg aatggcgtgt tgcatcggcg
     2401 tattggcaca taaacgcata gaagagagca tatccaaccc ttcaataaaa cctttggctt
     2461 gatgcttcgg accgctcagc accatccagc cctgacggaa accggcaaca cgatacgcct
     2521 tggataaacc gttataagtt acggttaata cgtccggtgc aagagcagca atatggtgat
     2581 gtaccgcacc atcatagaca attttttcat aaatttcgtc agcaaaaata attaagttat
     2641 gctgacgggc aagttctgca atttccagca aaatttgacg gctatacact gctccggtcg
     2701 ggttattcgg gttgataacc aaaatccctt tagtgcgagg cgtgattttc gatttaatat
     2761 cttcaagatc agggaaccac tcattttctt catcgcacag gtaatgcact gctttaccac
     2821 cggctagggt tgaagctgct gtccataacg gataatcagg cattggaatt agaatttcat
     2881 cgccatcatt taataacgcc tgcatcgaca ttgtaatcag ttcagatacg ccgttgccga
     2941 tgtaaacatc gtttacatcc ataccacgca taccttttga ttgataatat tgcacaatcg
     3001 ctttgcgggc agaatacaaa ccttttgaat cacaatagcc ttgtgcggtt ggaagattac
     3061 gaatcacatc cactaaaatt tcatcgggtg cttcaaagcc aaaaggggct gggttaccga
     3121 tatttaattt taaaatttta tgcccttctt cttctaagcg aagtgcctct ttatgaattg
     3181 gcccacggat atcgtaacga acctgagcta acttatctga tttgggaaaa cgatccattg
     3241 taaacttcct ataatgtatt atctaattgt agtctcatcc cgctgttcaa aaaatcggta
     3301 attcggaatg aatttgtaaa cattactcta atttgggagg aaaaaaaaga ggaaatacgt
     3361 taaaatggag accttttttt caatcagcta aaaacaagcg gtttcttttt ctgatttttt
     3421 tgcaaatgct tatttttcat caattaaaac aagaacttac aaaaagttac tcaaaagtgg
     3481 gaagagagca aggattggtc gccattcaag cctctatgcc gctattggag gcaaatattc
     3541 cgctactcgg ttggttgaaa acacaaaaaa caagctaccc tcactttttt ctgcaatatc
     3601 gtgatacaga tcagaccatt gccaccattg gcacacttcg ccaattttat acgctagaag
     3661 ctgcacaatc ttttattcaa caacatcatt tcccgttaat cggcgggctt caatttgaag
     3721 gcaatacgca atttattttg cctcaatttt cattggtaaa aaatcagcaa aatttaaccg
     3781 cttgcttcta ttttgatagt gataactttg aacaacaagc ggttattttt gaacaatttc
     3841 ttgcaaattt cagccaaatt accgaattag gactaacgga taatccgctc gtttcaatga
     3901 gtgccaccag ccattttgag caatggaaag ccaatattga aaaagcgatt atttctattg
     3961 agcagggcga ttttcaaaaa gtggtattgg caaatgcgat gagacttcat tttgagcaac
     4021 ccatttgtgc ttacagcttg ctggcacaaa gccaaaaaac aaatttaggt tgttaccact
     4081 ttttatgggc ggaaagtgct gatgttgcct ttcttggctc aacccctgag cggctatatc
     4141 aacgccaagg gctatacttt tctaccgaag ccctcgccgg caccgctgcg gttaccacca
     4201 gtgaagtgca aaccgaactc aatgcacaat ggctgttaag tgatcccaaa aatatctatg
     4261 aaaatcaatt agtggttgat gacattgaaa accaactcaa agattgcgtg accgaatttg
     4321 cagtatccga ggcaaaaatt aagcgtttgc ataatgtgca acacctacgc cgccaaattc
     4381 aagcaacgct caaaccggac acctcagatt cagattgctt aagcaaaatc cacccaactg
     4441 cggcagttgc agggcttccc cgccaaaaag caaagggttt tattaccgaa aatgagcctt
     4501 ttacccgcca ttggtatgca ggtacattag gcgtaatggg caacgaggaa gccgaatttt
     4561 gcgtaacact ccgttctgca ttaatttcac aaaattcaat tacgctttat gccggtgccg
     4621 gcattgtggc aggctcagat ccacaatcgg aatggcaaga aattaaacgt aaggcacaag
     4681 gaatggcaaa actgttggat gttgatttag taacaaaagg ggaactgtaa gctagacttt
     4741 cagaacttaa tctcttaatt atttctttct atattttcat ttccaacttc ccaacaaaat
     4801 atattattgc caccaaatta gtagtgaata ttccataatt aaaaaatt
//
LOCUS       DANKBV010000153         4755 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.153, whole genome shotgun sequence.
ACCESSION   DANKBV010000153 DANKBV010000000
VERSION     DANKBV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.153"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..514
                     /locus_tag="PWY43_002180"
     CDS             <1..514
                     /locus_tag="PWY43_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137025.1"
                     /translation="DESERIISASIEFSNYFANKYSENIEEGIKNAEKEFKKNYNKSL
                     RYDAKKAQQRVQKYVEKVNQKNPQALKDLDNISNLKKINENLKKAAKFTGIISSIKDY
                     YDLAEGFRNGIKSNDWEPFLKTSAQIGASALMSMLVASMITGPTTIGSIIISAAFISI
                     GGYIISLASR"
     gene            990..3029
                     /gene="malQ"
                     /locus_tag="PWY43_002181"
     CDS             990..3029
                     /gene="malQ"
                     /locus_tag="PWY43_002181"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL2137026.1"
                     /translation="MSNKQNTAHYFYSPNIKEYASPSAFEKIERLLNTDNDLQKNNQI
                     LPLVKVITQPNAEQQAVEISLNFTNVAKWQIVLENGEVRNGEIKAEQSVLTLPADLPL
                     GYHQLSISTENDEESCSLIVTPPKAFQPKPLEERKKLWGAFLQLYTLRSDNNWGIGDF
                     GDLKQFLANISDYGADFVGLNPIHALFPADPDAASPYSPSSRLWQNIIYIDVCAVEAF
                     QQSEQAQQWFNSEETQRQLAELRAKDYVDYSQVAQLKLKALQFAFEHFSQPSQQQSQT
                     EFNAFIEQAGEPLKIQATFDALHQWISSEFGEKGGWTNWAEEYQSYHSPKVAEFQTSQ
                     ATQIRFYMWLQFVANQQLAECNQFAQSLSMPIGFYRDLAVGVTQSGAETWADKALFVQ
                     EASIGAPPDMLAPNGQSWGLSPMHPHILQSRGYQPFIDLVRANMKHCGALRIDHILGF
                     ARMWWVANGESAKEGIYVRYPLEDLLSILALESQRHQCLVIAEALGIVPEGILDSLEQ
                     KGIFAYNVFYFEKAENAFKPLEDYPYQAMTTLSTHDLPTIQGYWKGYDFELGEKFNTY
                     PSKAVLRKLKEERSYDRLNIRNAVEKVMELEPNEVGVTMKFTHQLQRYVAHTHSALFG
                     TQPEDWLNMLEPVNIPGTSTEYPNWRRKLSATTEQIFANKDIQQLLKDINEIRKG"
     gene            complement(3097..3828)
                     /locus_tag="PWY43_002182"
     CDS             complement(3097..3828)
                     /locus_tag="PWY43_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDL2137027.1"
                     /translation="MGCSSFSTKLKQEFFSGWTTFEASWLIVFLAIQIGIFIYQPDTW
                     IATIAAITGILCVVFVGKGKISNYLFGLISVSLYAYISYTFQLYGEMMLNLLVYVPVQ
                     FIGFYFWRKNMTSENTVNNAGVEEVIAKALTAKQWVIVAITTIIGTFLYIELLKYLGS
                     ALAILDGATVVISIVAQILMVLRYREQWALWIIVNMMTIALWTAMYFQNGETSLPLLV
                     MYVMYLCNSIYGYYNWIKLHRKHQQ"
     gene            4228..>4755
                     /locus_tag="PWY43_002183"
     CDS             4228..>4755
                     /locus_tag="PWY43_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL2137028.1"
                     /translation="MNKIYRIVWNHAKRTWIVASKLSRSANKSNATNTSLTTNIIKLS
                     TLSLALSAGFASAATYSPTYGTIPDNNPQTSIAIGDGSKVNSTLVSTITGLEGNSGKV
                     STALGYNTTATADFSTALGGFANASFASTAVGGQATADKRSVAVGYNATAMGLREVFI
                     GDHAGLNHSNSTEYNI"
BASE COUNT         1666 a          916 c          840 g         1333 t
ORIGIN      
        1 agatgaatca gagaggatta tatcagccag tattgaattc agtaactatt ttgctaataa
       61 gtactctgaa aacattgaag aaggtattaa aaatgcagaa aaagaattta aaaagaatta
      121 taataaaagt ctaagatatg atgctaaaaa agcacagcag agggtgcaaa aatatgtcga
      181 aaaagtcaac caaaagaatc ctcaggcact aaaagatctc gataacatat ccaacttaaa
      241 aaaaattaat gagaatttaa aaaaagcagc aaaatttact ggtattatta gcagtattaa
      301 ggattattac gatctagcag aagggtttag aaatggcata aagtctaatg attgggaacc
      361 ttttttaaag acatctgctc agataggtgc ttcagcttta atgagtatgt tagtagcctc
      421 aatgataaca ggcccaacga caattgggag tattattatt agtgctgcat ttatttctat
      481 agggggatat attatttcac tagcttcccg ttagatagtc tttgttatac tcaatagcct
      541 actatactac ctataggcta ttgaactatc atacatcagc tctattaacc aaaaaaacaa
      601 aaaaaaatat tgcaaataaa aataaaatta ttgagatagg tccagagtac ataattaact
      661 catacttttt tttaagttta atcctaagag cctgagctac aaaaggatat aaaaataaat
      721 taaccagcaa atataataga gttataaaat aaatgaaaaa tgtatttgaa ttccaggcct
      781 caaagctacg caaatcagaa aaaagcacag cacaggatat taataaaaac aaaaatggta
      841 tatttaataa taaaacataa ccctttagag ttaaattaaa tttttttaaa atcatatcac
      901 aaagacaaac ctgttgccta ctcttcttac tcatattaat cttcacaaga tcgttatttt
      961 aacaaataca gtaaaggaaa aaagtcataa tgtcaaacaa acaaaataca gctcactatt
     1021 tttactcccc aaatattaaa gagtatgcca gcccctctgc ttttgagaaa atagagcggc
     1081 ttttaaatac cgataacgat ttgcaaaaaa ataatcaaat tctaccgctt gttaaagtga
     1141 ttactcagcc aaatgccgag caacaagcgg tcgaaatttc gctgaatttt acaaatgtag
     1201 caaagtggca aattgtgctt gaaaatggtg aggttcggaa cggtgaaatt aaggcagagc
     1261 aatcagttct aaccctgcct gccgatttgc ctttaggcta tcatcagctt tctatttcaa
     1321 ctgaaaatga tgaagaatct tgctcgctga ttgtaacacc gccgaaagct ttccagccta
     1381 agccattgga agaaaggaaa aagctctggg gtgcattttt acagctttat actttgcgtt
     1441 ccgataacaa ctggggaatt ggcgattttg gcgatctcaa acaattcctt gccaacattt
     1501 ctgattatgg tgcagatttt gtgggcttaa accctattca cgcccttttt ccggcagatc
     1561 ctgatgcggc aagcccttac agcccgtcct ctcgactttg gcaaaatatt atttatattg
     1621 atgtttgtgc agtagaagcc tttcaacaat ctgaacaggc tcaacaatgg tttaacagtg
     1681 aagaaactca acggcagctt gctgaattgc gagcgaaaga ttatgtcgat tacagccaag
     1741 tagcgcaact gaaattaaag gctctgcagt ttgcttttga gcatttttca cagccatctc
     1801 aacaacaatc gcaaaccgag ttcaatgcct ttattgagca agcgggcgag ccattaaaaa
     1861 tccaagcgac gtttgatgcc ttgcaccaat ggatttcatc tgaatttggc gaaaaaggcg
     1921 gctggacaaa ctgggcggag gaataccaaa gttaccattc gcctaaagta gcagaatttc
     1981 aaacctcaca ggcaacgcag atacgttttt atatgtggtt gcaatttgtg gcaaaccagc
     2041 aattagcaga atgcaaccaa tttgctcaat cactttcaat gccgattggt ttttaccgcg
     2101 atttagccgt tggtgttacc caaagcggag cagaaacgtg ggcggataaa gccttatttg
     2161 tgcaagaggc gtcaatcggt gcaccacctg atatgctcgc cccgaacggg caaagctggg
     2221 ggctttctcc aatgcacccg catattttac aaagccgagg ttatcagccg tttattgatt
     2281 tagtgcgtgc taatatgaaa cattgtgggg cattgcgaat cgaccatatt ctcggctttg
     2341 ctcgtatgtg gtgggtggcg aacggtgaat cggcaaaaga agggatttat gtccgttatc
     2401 cgctcgaaga tttgctctct attttggcat tagaaagcca acgtcaccaa tgtttagtga
     2461 ttgcagaggc tcttggcatt gtgcctgaag gtattttgga ttctctcgag caaaaaggca
     2521 tttttgccta caatgttttt tattttgaaa aggcagaaaa cgcgtttaaa ccgctggaag
     2581 attatcctta ccaagcaatg acaaccctaa gcacccacga cttaccaacc attcaaggct
     2641 attggaaagg ctatgatttt gagctaggcg aaaaatttaa cacttaccca agcaaagccg
     2701 tattgagaaa gctgaaagaa gagcgttcgt atgaccgctt gaatatccgt aatgcggtgg
     2761 aaaaagtgat ggaactcgag ccaaatgaag tgggggtcac aatgaaattt acccatcagc
     2821 tacaacgcta tgtggctcac actcacagtg cattattcgg tacacagccc gaagattggc
     2881 tcaatatgtt agagccggtg aacattccgg gaaccagcac cgaataccct aactggagaa
     2941 gaaaattatc cgcaaccacc gagcagattt ttgcaaataa agatattcaa caactgctaa
     3001 aagatataaa cgaaattcgt aaaggataat aacaagcggt catttttagg taaattttta
     3061 caaaaaatga ccgcttgtaa tttgtattta ttcgctctat tgctgatgtt ttctgtgaag
     3121 tttaatccag ttgtaataac cgtaaataga gttacataaa tacatcacat acatcaccaa
     3181 cagcggcaag ctggtttcgc cgttttggaa gtacattgcc gtccataatg caatggtcat
     3241 catattcaca attatccata atgcccattg ctcacggtag cgaagtacca tcaaaatttg
     3301 tgccacaatc gaaataacca ctgttgcacc atctaatata gctaacgcac tgcctaaata
     3361 tttaagcaat tcaatataaa gaaatgtgcc gataatggtg gtaatggcga caatcaccca
     3421 ctgttttgcg gttaaagctt tagcaattac ctcttccaca ccagcattat ttaccgtgtt
     3481 ttcgctggtc atatttttac gccagaaata gaaaccgata aactgcaccg gcacatatac
     3541 aagtaaattc agcatcattt caccatataa ctgaaaggta taagagatat aagcataaag
     3601 cgaaacggaa attaagccga atagatagtt actgattttt cctttaccga caaataccac
     3661 gcataaaata cctgttattg ccgcaatagt tgcaatccaa gtatcgggtt gataaataaa
     3721 aataccgatt tgaatcgcta aaaaaacaat caaccaactt gcctcaaaag tggtccaacc
     3781 actaaaaaac tcttgtttta acttagttga aaaagatgaa cagcccatat ttcctccata
     3841 taaagtgaag tattcttttc aaattctaag ttatttaaat taagaaatca aaatacagag
     3901 taccttttga taaatatacg aaacatttat tatattaata aacctatatt taattggcat
     3961 ttaactaaat cagtgttttt taaaactaaa aaacatacca aaaaccaaat aaaactctgc
     4021 actccacctc aatattagta caaaacataa aatttaaaat aatacattta tatcaaaaaa
     4081 ttgctatatt tattcctaaa aagccactat ttacaccata aaacctcacc tatataattt
     4141 ttcagcctat tgtatatatt tggctaggca atactcaaat cattaaaatt attaaaatta
     4201 ttaaaaaata agaacaggag tagttctatg aataaaattt atcgcattgt ttggaaccat
     4261 gcaaaacgaa cttggattgt tgcttctaaa ttatcaagaa gtgctaataa atctaatgca
     4321 acaaatacat cactaaccac taatattatt aaactcagca cactaagtct tgcgctttct
     4381 gcaggttttg caagtgctgc aacctattca ccaacctatg ggaccatacc agataataac
     4441 ccacaaacat ctatcgcaat tggtgatgga tctaaagtta actcaacact agtaagcaca
     4501 ataacaggct tagaaggaaa tagcggtaaa gtttctacag cattagggta taacacaacc
     4561 gcgacagccg atttttcaac tgcattagga ggctttgcta acgctagttt tgcttcaaca
     4621 gctgttggtg ggcaagccac tgccgataaa cgttccgtag cagtagggta caatgcaacc
     4681 gcaatgggct tacgtgaagt ttttattggc gatcatgccg gattaaatca ctctaacagc
     4741 acagaataca atatt
//
LOCUS       DANKBV010000154         4663 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.154, whole genome shotgun sequence.
ACCESSION   DANKBV010000154 DANKBV010000000
VERSION     DANKBV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.154"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..214
                     /locus_tag="PWY43_002184"
     CDS             <1..214
                     /locus_tag="PWY43_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL2137029.1"
                     /translation="VFVDLGSAILSTQTAIDLLEPELAEKVIISYAPLVEGAFAAVVA
                     ASAGDSLEAVLNEANEAAALKQEQAN"
     gene            complement(254..1099)
                     /locus_tag="PWY43_002185"
     CDS             complement(254..1099)
                     /locus_tag="PWY43_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HDL2137030.1"
                     /translation="MDYFVNVNEQNFSEVWAAAAQVPTVFHFISPREVASLEMDALLR
                     RISDENPNQFLLATVNCDEQQALAAQFRIQQIPTLYLFAGGQPIDAIQGAVSEQELRI
                     RLANILPKEEELKFQQALELLQDQRYTEALPLLKSAWEMTDKKNSDFALLYAETYIAM
                     KSVEEAREILKAIPIQDRDSRWHGLQAQIELLEQASESPELQQLQADYANSKTPEIAI
                     KLANQLHQAHKNEEALTLLFDWLKSDLNVANGEVKSQFLAILSALGTDPIVPKFRRQL
                     YSLLY"
     gene            complement(1143..1469)
                     /gene="glpE"
                     /locus_tag="PWY43_002186"
     CDS             complement(1143..1469)
                     /gene="glpE"
                     /locus_tag="PWY43_002186"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDL2137031.1"
                     /translation="METTFKEISPQQAWELIENEGAVLADVRDARRYVYSHPQDAFHL
                     TNQSYGKFLDEVDYDEPVVVICYHGVSSQSTAQFLIEQGFEKVYSVKGGFEAWERNGL
                     PIETSY"
     gene            1620..2078
                     /gene="exbB"
                     /locus_tag="PWY43_002187"
     CDS             1620..2078
                     /gene="exbB"
                     /locus_tag="PWY43_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HDL2137032.1"
                     /translation="MEQLFQFLEHHTDFVILGLLGLLSVICLWKAIERIMFYSKVDIS
                     KYDTLEELDIDLTHNLTTISTIGSNAPYIGLLGTVVGILLTFYHLGQSGGDIDAASIM
                     VNLSLALKATAVGILVAIIAMVFYNGFGRKVEENKLKWLALQSKKPHQAV"
     gene            2115..2552
                     /gene="exbD"
                     /locus_tag="PWY43_002188"
     CDS             2115..2552
                     /gene="exbD"
                     /locus_tag="PWY43_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDL2137033.1"
                     /translation="MKKFDEINIIPFIDIMLVLLAIVLVTASFISQGKIQVNVPKAST
                     TQPMKADDLAKLLTITENNEFFFNDQAITKEQLIAEVATWDKSQKVSLKVDGAVAFEK
                     FVELTDILSANEIKNVAIITKKETAPAPSSTPGSPAQVPAVAP"
     gene            2568..3467
                     /locus_tag="PWY43_002189"
     CDS             2568..3467
                     /locus_tag="PWY43_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDL2137034.1"
                     /translation="MKKKHSRIGLLASLAIHTALIGGTFAWLHNHKVEKIAEQNSLSM
                     EMVAALLEQPQVAVAPDPITEEVKEIEPEKAEVPPEPKLTPEPTPEPEAIPDPTLNPK
                     KEKPKEEKRKERHHHKHKHRHKEKREEEKPKDKPKERPKHHHKHIKALERGPEIKQGI
                     VAKAIPNAAEGEKVVAGVVGGQKNGSPNSTSTTGSANTGASSGNASELAAYKVALQRA
                     LQRKANNSYPQREKMMRKTGTVTIKFTVTSSGSITNVSVLNSSGNSNLDNAAVRSAEG
                     LSMPPPPAGFPPTVTVPVRFALE"
     gene            complement(3536..4417)
                     /gene="cysZ"
                     /locus_tag="PWY43_002190"
     CDS             complement(3536..4417)
                     /gene="cysZ"
                     /locus_tag="PWY43_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDL2137035.1"
                     /translation="MSSVFSEPQNQVGLGFHYFIYGWRLLLQRQFLPFVIFPILINTV
                     LMVGLIWGFFANIGGLLDMLLPSWLEWLSFVLIPLIFLMILVGFYFAFTTLANFVAAP
                     FNALLAEKVELQLTGEPLGDMSFGAMLKDVPRMLKREWQKMMYSIPRLIALFVLGFLP
                     VVGQTVAPVLTFIFGAWLIAIQYCDYPFDNHKISFQRMRNALSQSRTLNFTFGALVSL
                     FTMIPFVNLVVMPVAVCGATALWVKEYRNFFLNNQTGEFSKADYTFTKVNSTSLSTET
                     RTGEVSPNVRNGDIRKQ"
     gene            4529..>4663
                     /locus_tag="PWY43_002191"
     CDS             4529..>4663
                     /locus_tag="PWY43_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDL2137036.1"
                     /translation="MELHIIFFILAGLLIAVLIGFSLWSARREKSNIFSNTFSTRPIA
                     V"
BASE COUNT         1479 a          985 c          882 g         1317 t
ORIGIN      
        1 tgtgtttgtc gatttaggca gtgcaatttt aagtacccaa accgccatag atttactgga
       61 gccggaactt gccgaaaaag tgatcattag ctacgctcct ttagttgaag gtgcttttgc
      121 tgctgtagtt gcagcctctg ccggcgatag tttagaagcg gtattaaatg aagcgaacga
      181 agccgctgct cttaagcagg aacaagccaa ttagtaaaaa tgccgtatcg aaaaatacgg
      241 cattttagtt ttattagtac aatagtgaat aaagctgtct tctaaatttc ggcacaatcg
      301 ggtctgtacc cagtgcagat aagattgcca gaaattggct ttttacctcg ccgttcgcta
      361 catttaaatc cgattttaac caatcaaaaa gtaacgtgag tgcttcttca tttttatgag
      421 cttggtgtag ctgatttgcc aatttaatcg caatttcagg ggttttgctg ttcgcataat
      481 ccgcttgaag ctgttgtaat tcgggcgatt cgctggcttg ttccaataat tcgatttggg
      541 cttgcaaacc gtgccaacgg ctgtctctgt cttgaatcgg gatcgcttta aggatttcac
      601 gagcctcttc cacgcttttc attgcaatgt aagtttcggc atagagcaaa gcgaaatcgc
      661 tgtttttctt atcggtcatt tcccacgctg atttcagcag cggcaaggct tcggtataac
      721 gctgatcttg caataattcc aaggcttgtt gaaatttcag ctcttcctct ttcggtaaaa
      781 tatttgccaa ccgaatccgt aattcctgct cacttactgc accttgaatt gcatcaatcg
      841 gctgaccgcc ggcaaacaaa tagagcgttg gaatttgttg aatgcgaaat tgagcagcca
      901 atgcttgttg ctcatcacaa tttaccgtcg ccaataaaaa ttggttaggg ttttcatccg
      961 aaatacgacg tagtaaggca tccatttcca atgaagccac ttcccttggc gaaataaaat
     1021 gaaacaccgt cggcacttgt gccgctgctg cccatacttc actaaaattc tgctcgttta
     1081 catttacaaa gtagtccata caaaaccttc ttaagctaaa aattactttt tatattgtag
     1141 cattaataag aagtttcaat aggtagcccg tttctctccc acgcttcaaa accacctttt
     1201 acactataaa ctttctcaaa cccttgttcg attaaaaact gtgctgtact ttggctactc
     1261 actccgtgat agcaaatcac aaccaccggt tcgtcataat cgacctcgtc taaaaactta
     1321 ccataactct ggttggttaa atgaaacgca tcctgtgggt ggctgtaaac ataacggcgg
     1381 gcatcacgca catcggcaag caccgcacct tcgttttcga ttaattccca agcctgttgc
     1441 gggctaattt ctttaaatgt agtttccata atcactttcc ctcaaaaaac gtgcaactat
     1501 accataaaat tctaaaaaga atggctatta ttccaagttg aaaatttgat gtttgtcaga
     1561 tataattgca aactattttc aaatgcactg atttaaaact accacagttt gaggatttta
     1621 tggagcaatt atttcaattt ttagaacatc atactgactt tgttatatta ggattactag
     1681 gcttattaag cgtaatctgc ctttggaaag ctattgaacg tattatgttt tattccaaag
     1741 tagatatttc aaaatacgat accctcgaag aattagacat cgatttaacc cacaatttaa
     1801 ctaccatttc gactatcggc tctaatgccc cttatatcgg cttattagga accgttgtcg
     1861 gtattctttt aactttctat catttaggtc aatcaggtgg tgatattgat gcggcctcta
     1921 ttatggtaaa cctttcttta gctctaaaag ccactgctgt cggtatctta gtggcaatta
     1981 ttgcaatggt tttctacaat ggttttggtc gtaaagtgga agaaaacaaa ctgaaatggt
     2041 tggcattaca atcaaaaaag cctcatcaag cggtgtgatt tagtccattt tttgcaaatt
     2101 aataaggcag aacaatgaaa aaattcgatg aaatcaacat cattccattt attgatatta
     2161 tgttggtatt actggctatc gtgttagtta ccgcctcttt catttctcag ggtaaaatcc
     2221 aagtaaatgt accgaaagca agtacaacac agccaatgaa agcagacgat ttagcgaagc
     2281 ttttaaccat taccgaaaat aatgagtttt tctttaatga tcaagcaata actaaagagc
     2341 aattaattgc tgaagttgcc acttgggata aaagccaaaa agtgtcactc aaagttgatg
     2401 gtgcagtcgc cttcgaaaaa tttgtggaat taaccgatat cctttctgca aatgaaatta
     2461 aaaatgtggc aatcattact aaaaaagaaa ctgctccagc accttcaagc acacctggta
     2521 gccctgcaca agtgccagca gtcgcacctt aaatagggta aacaggtatg aaaaaaaaac
     2581 attctcgtat cggcttattg gcatctttgg ctattcacac tgccttaatt ggtggcactt
     2641 ttgcttggtt acataaccac aaagtggaaa aaattgccga gcaaaactct ctttctatgg
     2701 aaatggtggc agcattatta gagcaacctc aggttgccgt tgctccggat cctattaccg
     2761 aagaggtcaa agaaatcgaa ccggagaagg cagaagtacc acctgaacct aagcttactc
     2821 cggagccaac tccagagccg gaggcaattc cagatcctac gctaaatccg aaaaaggaaa
     2881 aaccgaaaga agaaaagcgt aaagagcgac accaccataa acataagcat cgtcacaaag
     2941 agaaaaggga agaagaaaaa ccgaaagata agcctaaaga gcgcccgaaa catcatcaca
     3001 agcatattaa agcattagag cgcgggccag agataaaaca aggtattgtc gccaaagcga
     3061 ttccaaatgc ggctgaaggt gaaaaagtgg ttgcgggggt tgttggaggg caaaaaaacg
     3121 gttcaccaaa ttcaacctca actacaggtt cagcaaatac tggggctagc agtggtaatg
     3181 ccagtgaact tgctgcttat aaagtcgcat tacagcgagc attacagcgt aaggcgaata
     3241 actcttaccc tcagcgtgaa aaaatgatgc ggaaaaccgg tacagttacg attaaattta
     3301 ccgttacatc gtccggttca attacaaatg tatccgtact caattcatcg ggaaatagta
     3361 atttagataa cgctgcggta cgcagtgcag aaggtttgag tatgcctccg ccaccggcag
     3421 gcttccctcc tacggtaaca gtaccggtaa gatttgccct tgaataaaaa cagccctcaa
     3481 aagcaatgta cttttgaggg ctttttattg caaaaaattg actaaaaatg accgcttact
     3541 gcttgcgaat atcgccattt ctcacattcg gactcacttc acccgtacga gtttctgtgc
     3601 ttaacgaggt agaatttact ttcgtaaaag tataatccgc tttgctgaat tcacctgttt
     3661 gattatttaa gaagaaattg cggtactctt ttacccataa tgccgttgcg ccacaaactg
     3721 ccacaggcat aactactaaa ttcacaaatg gaatcatcgt gaataggctc actaaagcac
     3781 caaaggtaaa atttaaagtg cgagactgcg acaacgcatt tcgcatacgc tgaaaactga
     3841 ttttatggtt atcaaacgga tagtcacaat attgaatggc gatcaaccaa gcaccgaaga
     3901 taaaagtcag cacaggtgct acggtttgcc caaccacagg caaaaagcct aatacaaaaa
     3961 gagcaatcag ccttggaatg ctatacatca ttttttgcca ttctcgtttc agcattcgag
     4021 gcacatcttt taacattgca ccgaatgaca tatcgcccaa aggctctccg gtaagttgca
     4081 gttcgacttt ttctgctaat aacgcattaa agggagcagc aacaaagtta gccaaggtag
     4141 taaaagcaaa ataaaatccc actaatatca tcaaaaaaat cagtggaatc agtacaaagc
     4201 taagccattc cagccagctt ggcagtaaca tatccaacaa gccaccgata ttggcaaaaa
     4261 atccccaaat cagccctacc atcagcacgg tattaataag aattgggaaa atcacaaaag
     4321 gcaaaaattg acgttgtaac aataaccgcc agccatatat aaaataatga aagcctaatc
     4381 cgacttgatt ttgcggctca gaaaaaactg aagacataat gattccttat tttctatcaa
     4441 aattaattag aaatatggta agctacgcac attattttcg caaatatgaa aatacatttt
     4501 ttattgatta tttgataaag aggacacaat ggaactgcat attatttttt ttattcttgc
     4561 cgggctactg attgctgtac taattggatt tagcctttgg tccgctcgtc gtgaaaaatc
     4621 aaatattttt tcaaatacat tctcgaccag acctattgct gtg
//
LOCUS       DANKBV010000155         4606 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.155, whole genome shotgun sequence.
ACCESSION   DANKBV010000155 DANKBV010000000
VERSION     DANKBV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.155"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..789)
                     /locus_tag="PWY43_002192"
     CDS             complement(<1..789)
                     /locus_tag="PWY43_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2137037.1"
                     /translation="MSYKVVFSLLFSLFLSTANANWTKINDVNYTWGPFNIYHITLFT
                     ESGTYVEGTRPLMLTLQYKKPWEGRDFAVSLARTWNNLGIKLPEKELDEVITHLRKTF
                     PDIKPEDTLHYIALEDRGYFILNDKVVSDVFNKAFNDAIVAIWLDPKMDISHQLLDPS
                     YKPRVESKNIESQAEEKSAEVKAQEPIANTEVSKEMKESQTVEQESNKTETLEVDKTQ
                     SSVANEATQPVENKDKKEPEKLEEKPKEMKIENPEVFISPIYDDI"
     gene            979..1194
                     /locus_tag="PWY43_002193"
     CDS             979..1194
                     /locus_tag="PWY43_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDL2137038.1"
                     /translation="MFPEFRDLITQLKQNNSHFARLFDKHNELDQRIKNMEANIELAT
                     YEEVEALKKEKLHLKDQLYAILKQESA"
     gene            complement(1253..2410)
                     /locus_tag="PWY43_002194"
     CDS             complement(1253..2410)
                     /locus_tag="PWY43_002194"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL2137039.1"
                     /translation="MSLDLADIRTQITNLDRNLLKLLAERHRLAFDVVRSKEITQKPL
                     RDLEREKILLQELVNFAEAENYQLDPQYVTQIFQRIIEDSVLTQQNYLQNKLNAQKEE
                     QVSIAFLGMRGSYSNMASRQFAKKYQGTLAELSCHSFNEVFEKVSLGEADFGVLPLEN
                     TTSGSINEVYDLLQHTDLTLVGELAYPIKHCVLANGTVDLAEVDTIYTHPQPAQQCSQ
                     FLAGLDKVHIKYCESSSHAMQMVARLNKPNIVALGNEDGGKLYGLTNIKTDIANQAHN
                     ITRFIVVAKEAVQVSPQVQTKTLLLMTTTQQAGALVDALMVFKNHHIRMMKLESRPIY
                     GKPWEEMFYVELEANIHSENTQKALAELEQVTSYLKVLGCYPSEIIEPVKL"
     gene            complement(2532..4598)
                     /gene="prlC"
                     /locus_tag="PWY43_002195"
     CDS             complement(2532..4598)
                     /gene="prlC"
                     /locus_tag="PWY43_002195"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDL2137040.1"
                     /translation="MSNPLLNYTGLPAFSQIKPEHIKPAVEAVIKECRETVEKVAQIE
                     NPTWENFYMPQAMAGDKFSRAWSPVGHLNAVKNSPELREAYQACLPLLSEYSTWAGQH
                     QGLYQGYVKLKNSPEFETYSVAQKKAIENSLQDFELSGISLPAEKQKRYGEISARLAE
                     LNSQFSNNVLDATMGWDIVITDEAQLKGLPESALEAAKLSAESKGKKGYRFTLEIPSY
                     LPVMTYCENKALREEMYRAYVTRASDQGPNAGKWDNSAVIDEILKLRHEKAQLLGFKT
                     YSDYSLATKMAENPQQVLDFLNDLATRSKAQGKNELCDLKKFAKAHFGIEHLDLWDIP
                     FYSEKQKQALYSVNDEELRLYFPENRVLSGLFEVIKRIFGMRVEEQKEVDVWHKDVRF
                     FNIFDKNDRLRGSFYLDLYARENKRGGAWMDDCINQKRLADGTLQKPVAYLTCNFNKP
                     IGDKPALFTHDEVTTLFHEFGHGIHHMLTEIDVGDVAGINGVPWDAVELPSQFLENWC
                     WEEEALAFISGHYETGEPLPKAKLEQLLAAKNFQAAMFVLRQLEFGLFDFRLHTEYDP
                     SKQGQVLALLKEVKDQVAVAKGVEWARTPHSFSHIFAGGYAAGYYSYLWAEVLSADAY
                     SRFEEEGIFNPETGQSFLDNILTRGGSEEPMVLFERFRGRKPTLDALLRHKGILGNAE
                     PVDCTQ"
BASE COUNT         1293 a         1015 c          860 g         1438 t
ORIGIN      
        1 gatatcatca taaatcggtg aaatgaacac ttccggattt tcaatcttca tctcctttgg
       61 tttttcttct agcttttctg gctctttctt atctttattc tctaccggtt gagtcgcttc
      121 atttgccaca ctggattggg tcttatctac ctctagggtt tctgttttgt ttgattcttg
      181 ctctacagtt tgagactctt tcatctcctt agaaacctca gtattggcaa taggttcttg
      241 tgctttcact tccgctgatt tttcctcagc ttgtgattct atattttttg actcaactct
      301 tggcttataa gagggatcaa gtaattgatg gctaatatcc attttcggat caagccaaat
      361 tgctacaatg gcatcattga aagctttatt aaacacatca gagactactt tatcatttaa
      421 aataaaataa cctctatctt ctaatgcaat atagtgcaat gtatcttcag gcttaatatc
      481 tgggaaagtt tttctcaggt gggtaataac ctcatctagc tctttttcag gtagcttaat
      541 gcctaaatta ttccaagttc ttgctaaaga aaccgcaaaa tcgcgccctt cccacggttt
      601 tttatattgt agagtaagca ttaaaggacg tgtaccttct acataagtac cgctctcagt
      661 aaaaagcgta atatggtaaa tattaaatgg cccccacgta taattcacat cattaatttt
      721 tgtccaattt gcatttgctg ttgataaaaa tagagaaaac aacagcgaaa agaccacttt
      781 atagctcatt atttttgtgt ccttaaatct aaaattccta ctcattatta tagccttaaa
      841 tatggctcac acacattata attttcgata taaattttgt gatcaagttc aaaaaaatag
      901 aaagatttaa cagtaaatca acaaaaaaat gctagtattt aatcaagtaa aatttgcaac
      961 cactattagg agttaaatat gtttccagaa tttcgtgatc tcataaccca attaaagcaa
     1021 aacaattcac attttgctcg cttatttgat aaacataatg agctagacca gcgtattaag
     1081 aatatggagg caaatattga actggcaaca tatgaagaag ttgaggcact aaaaaaagaa
     1141 aaattgcatt taaaagacca actttatgca attctaaaac aagaatcggc ttaacctaaa
     1201 gtaaaagctc agaaaatatt ctgagcttta atttcggtca tattagacaa tattacagtt
     1261 ttaccggctc aataatttcg cttgggtagc aacccaacac tttgaggtag ctggtaactt
     1321 gctccaattc cgccaatgct ttttgggtgt tctcggaatg aatatttgcc tccagctcca
     1381 cataaaacat ctcttcccaa ggtttgccgt aaatcgggcg ggattccagt ttcatcatac
     1441 gaatatgatg atttttgaat accattaacg catcgaccaa tgcacccgcc tgttgcgtgg
     1501 tggtcattaa cagtaacgtt ttggtttgta cctgtggcga aacctgcaca gcctctttgg
     1561 caacaacaat aaaacgggtg atattgtgag cttgattagc aatatcggtt ttgatattcg
     1621 tcaagccata caattttccg ccatcttcat tgcccaaggc aacaatattc ggcttattca
     1681 agcgagccac catttgcatt gcgtgcgagc tgctttcgca atatttgata tgtactttat
     1741 ctagcccggc taaaaattgg ctacattgct gggcaggctg agggtgggtg taaatggtat
     1801 ccacctccgc caaatcaacc gtgccgtttg ccaatacaca atgtttgatt gggtacgcca
     1861 attcacccac taaggttaaa tcggtatgct gcaataaatc atacacttca ttgatcgaac
     1921 ctgaagtcgt gttttcaagc ggcagtacgc caaaatcggc ttcaccaagg ctgactttct
     1981 caaatacttc attaaaagaa tggcagctta attccgccaa tgtgccttga tattttttgg
     2041 caaactgtcg agatgccata ttggaataag agcctcgcat tcccaaaaac gcaatcgaaa
     2101 cctgctcttc tttttgtgca ttcaatttat tttgcagata attttgctga gtcagcactg
     2161 aatcttcaat aatacgttgg aaaatttggg tcacatattg cggatcaagc tggtaattct
     2221 ccgcttcggc aaaatttacc agttcttgaa gcaaaatctt ttcccgttct aaatctctca
     2281 agggtttttg tgtgatttct ttgctccgca ccacatcaaa ggctaagcgg tgacgttctg
     2341 ccagtagctt taataaattg cggtctaagt tggttatttg ggttcgaata tcggctaaat
     2401 ctaaagacat tatttcactc tctgtcaggc taaatgctga aaattataac gagttgcaaa
     2461 agaaagaaaa gacaagcggt caaatttaca aaaaaatttg caaatctaac cgcttgtaaa
     2521 acgtgtttac tttattgagt gcaatcaaca ggctcggcat tacctaaaat gcctttgtgt
     2581 ctgagcaagg catcaagggt tggcttacgc ccacggaagc gttcgaacag caccattggc
     2641 tcttctgaac cgcctcgggt taagatatta tctaagaaag actgccccgt ttcagggttg
     2701 aaaatcccct cttcctcaaa gcgtgaatag gcatctgctg aaagcacttc cgcccataaa
     2761 taactgtagt agcccgccgc atagccgcct gcaaaaatat gcgagaaact gtgtggcgtt
     2821 ctcgcccact ctacgccttt ggcaacagcg acttggtctt tcacctcttt aagtaaggct
     2881 aacacttgtc cttgtttgct cgggtcatat tcggtgtgta ggcggaaatc aaacaagcca
     2941 aattctaatt ggcgaagcac aaacattgcc gcttggaagt ttttcgccgc caataattgc
     3001 tccaatttcg cttttggtaa cggctcgccg gtttcataat gccctgaaat aaaggctaat
     3061 gcctcttctt cccagcacca gttttctaag aattggcttg gcaactccac tgcgtcccac
     3121 ggcacgccgt taataccggc aacatcgccc acatcaattt cagtcagcat atggtgaatg
     3181 ccgtgtccga attcatggaa caaggtagtc acttcatcgt gagtaaacaa tgccggttta
     3241 tcgccaatcg gtttattgaa gttacaggtt aaatatgcca ccggtttttg taatgtgccg
     3301 tcagccaagc gtttttggtt gatgcaatca tccatccacg ccccaccacg tttgttttca
     3361 cgagcgtata aatctaagta gaacgagcca cgcaagcggt catttttatc aaaaatattg
     3421 aaaaaacgta cgtctttgtg ccatacatca acctcttttt gttcttccac acgcataccg
     3481 aaaatgcgtt taatcacctc aaataagccc gataatacac gattttccgg gaaatacagg
     3541 cgaagttctt catcattgac tgaatacaat gcctgttttt gtttttcgct gtagaacgga
     3601 atatcccata aatctaaatg ttcaatgccg aaatgggctt tagcaaattt tttcaaatcg
     3661 cataattcat ttttgccttg agctttcgag cgagtggcta aatcgttcaa gaaatccagc
     3721 acctgttgtg gattttccgc cattttggtg gctaatgaat aatcagaata ggtcttaaag
     3781 ccgagtagtt gggctttttc gtgacgcagt tttaagatct catcaatcac cgcactatta
     3841 tcccatttgc ctgcattcgg gccttgatca gaggcacgag tcacataagc acggtacatt
     3901 tcctcacgca atgctttgtt ttcacaataa gtcatcaccg gcaaataact tgggatttca
     3961 agagtgaagc gatagccttt cttacctttg ctttctgcgg ataattttgc cgcttctaat
     4021 gccgattccg gcaagccttt aagctgtgct tcatcggtaa tcacaatatc ccagcccatt
     4081 gttgcatcaa gtacgttgtt gctaaattgc gagttgagtt ccgccaaacg agctgaaatt
     4141 tcgccgtagc gtttttgctt ctctgccggc agtgaaatgc ctgataattc aaaatcttgc
     4201 aagctgtttt caatcgcctt tttctgtgca accgaataag tttcaaactc agggctgttt
     4261 tttaacttca catagccttg atataaccct tgatgttgcc ccgcccaagt gctgtattcc
     4321 gataataacg gcaagcacgc ttggtaagcc tcacgcaact ctggggaatt tttcaccgca
     4381 tttaaatgcc cgaccggtga ccacgcacgg gagaatttat ccccagccat tgcttgtggc
     4441 atatagaaat tttcccacgt cgggttttca atttgtgcga ctttttcaac agtttcacgg
     4501 cattctttga tcaccgcttc taccgccggt ttaatatgtt caggtttgat ttgtgagaac
     4561 gcaggcaagc ccgtgtaatt taaaagaggg ttagacatat taatgt
//
LOCUS       DANKBV010000156         4580 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.156, whole genome shotgun sequence.
ACCESSION   DANKBV010000156 DANKBV010000000
VERSION     DANKBV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.156"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..666
                     /locus_tag="PWY43_002196"
     CDS             <1..666
                     /locus_tag="PWY43_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2137041.1"
                     /translation="VVFDGTKNQFIPCENQSEDKANYFEISDLDYIKAEEKGELVAVV
                     HSHPEPNGKPILSVLDRKMQVQTGLDWWLVHNRQIHKFRNVPHLIGREFKHGTMDCYT
                     LYRDAYMLAGYEMDEFERQDDWWHTGQNLYLDNIQGQGFERVETPQIGDVILMQVGAD
                     VPNHAAIYIGEQMVIHHSPNRLSKRDLYDGYWLRHTHSIWRHKLAEKLDFDGILNDIA
                     VNN"
     gene            935..1525
                     /locus_tag="PWY43_002197"
     CDS             935..1525
                     /locus_tag="PWY43_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL2137042.1"
                     /translation="MITVKFYGHLRKFGTDFRLEVKDTAEAIRALCSQLTGLRETLRD
                     GIYKVRIGKQYLDASALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFQAVLGVALIGAA
                     FFLGPVGWGVLGASSAMMMGAMGASMLLGGVAQMLTKMPNAPNIGNEKEKENSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETMTVEV"
     gene            1528..>4580
                     /locus_tag="PWY43_002198"
     CDS             1528..>4580
                     /locus_tag="PWY43_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDL2137043.1"
                     /translation="MGLFSRKRKNHTPKEAPETGRSKQIVNIVELLCEGEIEGLVDGF
                     KSIYLDGTQIQNDDGSYNFNNVSGQLNVGTQDQNVLDGYDSSQNEVSVGVEVKKKSGA
                     IVRTVTDERISRLRLTLGVRSLFHQNNQGDTNTTNVDLKITIGTRQYSHSFNGKYSSQ
                     YLESVVFDNLPPVPFNISVERVTKDSNSQRLQNGTIWSSYTEIIDTEFTYPNSAVAGI
                     SFDSEYFNNIPTRNYLIKAKKVKVPSNYDPVKRTYTGFWDGTFKIAWTNNPAWEIYDL
                     APILSKMLGVEISFDKWALYDVARYCDQLVPDGMGGMEPRFTCNVWLTEVKTAYDLLN
                     DFCSVFRAIPIWTGTEVSVIIDRPRDPVWTYTNANVVGGFERSYSARKSRHNAVQVTY
                     SDKTNGYESAIEYVSDDEEIKKHGLNLSQITAFGCTSRGQAHRTGKWILETEKREKET
                     ITFTVGREGLMHLPGDIIRVADSHYAGTEIGGRVLAINGRKVTLDREISIDNASYFTY
                     INGEATHSSIKIQSVNGKEITLDSTPTGLETYGVWSLSTKQISSGLYRSISIVENADG
                     TNTITALQHEPQKEAIVDNAAHFVETARTLYKAPQINAVEVSTGYDGKLYISSDISSG
                     DGKLTYDIKINKDGNLYQFKKGLADPNIELSDLPNGDYSVIIYGKNAKGQIVTEKTQT
                     FTIDRPPAPTGVVVTGGLGQITLEWDWVNEVTQTEIFAAETDNFALAKKIAKVTARTY
                     AHTLKGNKVVRYYWLRHTRGINVGPFYQQQGVRGQTAVDLDARLTELNTQLSRNIVNE
                     VFDVAAPARGLELVKTVANLTDKGTKLASSQVYNQADGKLYTWNGTAYSATVAAEDVT
                     GKLSKSKIDTSLISQLTGADNTANLARRLAETAQSNINQEITNRQDAVTAEANNRTKA
                     IQAESANLTKKIQAEATARGAAVTQLQNVDAQQAQLISAVTAKANNALSGLEEEKTAR
                     ANGDKAEAQAREALTARMGAAESNIVTIQRTVANNAQSISEVSQN"
BASE COUNT         1439 a          929 c         1047 g         1165 t
ORIGIN      
        1 gttgtttttg acggcacgaa aaatcaattt atcccttgtg aaaaccaatc ggaagacaaa
       61 gccaattact ttgaaatatc cgatcttgat tacatcaaag cagaagaaaa aggcgaactg
      121 gtagcggtgg tacattcaca tcccgagcca aacggcaagc ctatattatc tgtgcttgat
      181 cgcaaaatgc aggtacagac cggtttagat tggtggctag tgcataatcg gcaaatccat
      241 aaattccgca atgtaccaca tttaatcggg cgtgagttta aacacggcac aatggattgc
      301 tacacgctct accgtgatgc ctatatgctt gccggttacg agatggacga gttcgagcga
      361 caagatgact ggtggcatac cgggcaaaat ctatatttgg ataatattca agggcaaggt
      421 ttcgagcgag tagaaacccc acaaatcggc gatgtgattt taatgcaggt tggagctgat
      481 gtacctaacc acgcagcgat ttatattggc gaacagatgg taatccatca cagcccgaac
      541 cgattatcta agcgtgattt atatgatggc tactggttac gccataccca cagtatttgg
      601 cggcataaac tggcggagaa gttagatttt gatggcatat tgaatgatat tgctgtaaat
      661 aactaacaaa aaatgaccgc ttgtaacaaa aacaaacccc gaagcgtttg cagcacttcg
      721 gggtttttca tatccactta cctaactaag aggacataaa ttaaatgaat gataaacgat
      781 ttacattcaa atttctagga gttcttatgg aagcaattaa tattacccca aaagaaatca
      841 gaaaaacaat gtggacagcc gcaataattc tctttttgtt tgcgttcatc tggaaagctc
      901 ctgaatttat cactgctatt cgttggtggt aactatgatt acagttaaat tttacggaca
      961 tcttagaaaa ttcggtacag actttagact tgaagttaaa gacaccgcag aagccatcag
     1021 agcgttatgc tcacagctta caggcttgcg tgaaacgttg cgagacggta tttataaagt
     1081 ccgcatcggg aaacagtatt tagatgcctc agcccttgaa aaagggcttt tttattgcct
     1141 gaagaaaggg caaaccattc attttacccc tgttatcaaa ggggcgaaaa gcgggggtgt
     1201 tttccaagct gtgttaggtg tagccttaat tggagccgca ttttttctcg ggccggtcgg
     1261 ttggggagtt ctaggggctt cttccgcaat gatgatggga gcaatggggg catcaatgtt
     1321 gcttggcggt gttgctcaaa tgcttaccaa aatgccgaat gccccgaata tcggtaatga
     1381 aaaagagaaa gaaaactcaa cggctttttc aaatttaaat aacttggtgg ctcaaggcaa
     1441 acctgtgccg ttggcttatg gcttaattcg gaccggctcg cttgtgatat cgcagggagt
     1501 tgagacgatg actgtagagg tataattatg ggattattta gtcgtaagag aaaaaatcat
     1561 actcctaaag aagcaccgga aaccggtcgc tctaaacaga ttgtaaatat tgtggaattg
     1621 ctgtgcgagg gcgaaatcga gggcttggtt gatggcttta aatcgattta cttagacggc
     1681 acgcagatcc aaaacgatga cggcagctat aactttaata atgtgagtgg tcagttaaat
     1741 gtcggtacgc aagaccaaaa tgtgttagac ggttacgaca gctcacaaaa tgaagtgagt
     1801 gtgggtgtag aagtcaagaa aaaaagcggt gcaatcgtcc gcaccgttac tgatgaacgc
     1861 atcagccgtt tgcgtttaac gttgggcgtg cggtcactat tccatcaaaa taatcaaggc
     1921 gataccaata ccaccaatgt tgatttaaaa atcacgattg gcacacggca atattcgcat
     1981 agctttaacg gcaaatacag ctcgcaatac cttgaatctg tggtgtttga taacctgcct
     2041 cctgtaccgt ttaatatctc ggttgaacgt gtaacaaagg atagcaattc tcaacgacta
     2101 caaaacggta caatttggag cagctatacc gaaattatcg acaccgaatt tacttaccct
     2161 aattcagcgg ttgccggtat cagttttgat tcggagtatt ttaataatat cccgacccga
     2221 aactacctaa ttaaagcgaa aaaagtcaaa gtgccaagca attatgatcc agttaaacgc
     2281 acttacaccg gcttttggga cggcacattt aaaattgcgt ggacgaacaa ccctgcttgg
     2341 gaaatttacg atttagctcc gattttaagc aaaatgcttg gggttgaaat cagttttgac
     2401 aaatgggcgt tgtatgatgt tgctcgctat tgcgatcaac ttgtgccgga cggtatgggt
     2461 ggaatggagc ctcgtttcac ctgtaacgta tggctcacgg aagttaaaac tgcctatgac
     2521 ttgttgaatg atttctgctc ggtattccgt gcgattccga tttggaccgg aacagaagta
     2581 tcggtcatta tcgaccgtcc gcgtgatccg gtttggactt acacaaatgc taatgtagtt
     2641 ggtggatttg aacgatccta ctctgctcgc aaatcacgcc ataatgcagt gcaagttact
     2701 tattcggata aaacaaatgg ctatgaaagt gcgattgaat atgtctctga tgacgaggaa
     2761 atcaaaaagc acggcttaaa tttaagtcaa attactgctt tcggttgtac ctctcggggg
     2821 caagcacacc gtaccggaaa atggattttg gaaaccgaaa aacgggaaaa ggaaaccatt
     2881 acttttaccg tgggacgtga gggcttaatg cacttgccgg gtgatattat ccgtgttgcc
     2941 gacagccatt atgccggtac ggaaattggc ggacgtgtac tggcgattaa tggcagaaaa
     3001 gttacccttg accgtgaaat tagcattgat aacgccagtt attttaccta tatcaacggt
     3061 gaggcaacgc actctagcat taaaatccaa tcggtaaacg gtaaagaaat cacgctagat
     3121 agcacgccaa ccggcttaga aacctacggc gtatggtcgt tatcgactaa acaaatcagc
     3181 tcgggcttat accgctccat ctcgattgtt gaaaatgcgg acggtacgaa tacgattacg
     3241 gcattgcaac acgaaccgca gaaagaggcg attgtagata acgctgctca ctttgtggaa
     3301 acggcgagaa cgctttacaa agccccgcaa attaacgcag tagaagtctc gactggttat
     3361 gacggcaagc tctatatctc cagtgatatt agttctggcg acggtaagct aacctatgat
     3421 attaaaatca acaaagatgg taatttgtat cagttcaaga aaggattagc cgacccaaat
     3481 attgagttga gcgatttacc gaatggcgat tacagcgtga ttatttacgg caaaaacgcc
     3541 aaagggcaaa tcgtcaccga aaaaacacaa acctttacta ttgatcgacc tccagctccg
     3601 accggtgtag tagtaacagg tggattaggg caaattaccc tcgaatggga ttgggtaaat
     3661 gaagtcacac aaaccgaaat ttttgcggcc gaaaccgata atttcgccct tgcaaaaaaa
     3721 atcgcaaaag tgaccgctag aacctacgct cacacactaa aaggtaacaa ggttgtacgc
     3781 tattattggc tacgccatac cagaggcatt aatgtcggac cgttttatca acaacagggg
     3841 gtaagaggac aaacagcggt tgatttggat gctcgattaa ccgaattaaa cactcagtta
     3901 tcacgcaata ttgttaatga ggtttttgat gttgctgcac cggcaagagg tttagaatta
     3961 gtgaaaacag tcgctaattt gaccgacaaa ggtactaaac tggcgtcatc tcaagtttac
     4021 aatcaggctg acggcaagct ctatacttgg aacggtacag catactcagc tacagtggct
     4081 gcggaagatg ttaccggcaa attatcaaaa tctaaaattg atacgtccct tatttcgcaa
     4141 ttaacaggtg cagataacac cgcaaatttg gcaagaagat tagcggaaac agctcaaagc
     4201 aacattaacc aagagattac taaccgacaa gatgccgtta ctgccgaagc aaataaccgt
     4261 actaaagcca ttcaagccga aagtgcgaat ttaacgaaaa aaattcaagc tgaagcaacc
     4321 gctcgtgggg ctgctgttac acagttacaa aatgttgatg ctcaacaagc acaactcatt
     4381 tctgctgtca cagcaaaagc taataatgct ctatcaggct tggaagagga aaaaacagca
     4441 cgagcaaacg gcgataaagc tgaagctcaa gcacgtgaag cattaacagc aagaatggga
     4501 gcagctgaaa gcaatatcgt aactattcaa cgtacggtag caaataatgc ccaaagcatt
     4561 agtgaagtca gtcagaattt
//
LOCUS       DANKBV010000157         4503 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.157, whole genome shotgun sequence.
ACCESSION   DANKBV010000157 DANKBV010000000
VERSION     DANKBV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.157"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..147
                     /locus_tag="PWY43_002199"
     CDS             <1..147
                     /locus_tag="PWY43_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137044.1"
                     /translation="VMDSGTIHKVAHYFYGDYERADELLRLNPHIRQPNFIERGTLLN
                     CYWE"
     gene            150..1277
                     /locus_tag="PWY43_002200"
     CDS             150..1277
                     /locus_tag="PWY43_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2137045.1"
                     /translation="MENEIVVEIDGSQHKNWKSYDIDSDFLIPADSFNFELGKSSNIE
                     VLPNYSGKTAIVKINGKTVLTGIVDTTHHTISKSGRGFSLNGRDKAAILVDCSAPITN
                     VQGLSLLDAVKKIVTPLGIKEVQLKAEKNPTLSKVDIDVGESAWEAIMRCANSAGLHC
                     WFDPKGVLIVGGADYAQPPVATLYCVKESGDSRKNNFVDATLSYDVSQSYSEVTFLAQ
                     KHGKDSDNAKHDFKWVYKNEEMELYKPKTIVLGDVENLEALMKQAKKQVSDWQLESFN
                     LTIIVPDHKTKDGTLWQAGQRVHVVCEEYDLDGIFFLMGRRFRLSRTGGTTTELRLKL
                     DGVWTPDAYQAKAQEARKRKGKKGRKKKEPVEYVGSWELGK"
     gene            1282..1929
                     /locus_tag="PWY43_002201"
     CDS             1282..1929
                     /locus_tag="PWY43_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005711896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL2137046.1"
                     /translation="MRKLAKQTKEVAKGVQDSVRAAFRGVLNLVKSGSDIQQVQVSAL
                     ADETIQDLELMQHFGFTSVPPAGTQAVVIPLGGKTTHGIIVATENGSFRVKNLNDGEV
                     AIYDSSGSTIILKNGRVIDIECDSFNIKCKQYSVTASGGANFNTPKLETDQQFVSQGQ
                     ISGNGGMAVQGGEGASFSGNVNQTSGSFKTSGDVTAGTVSLKNHTHQSDGRGKPN"
     gene            2038..2388
                     /locus_tag="PWY43_002202"
     CDS             2038..2388
                     /locus_tag="PWY43_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDL2137047.1"
                     /translation="MDREISPLTRDYTSDNIATLQNAVYIRLTTPKGSWWADGRVGSL
                     LHLIQREKDLSRVGLLAQQYAEEALQPIIDDGRAKSITVTHEQPKDGSLNLLISVIDN
                     RGVKFEFKHPVKLV"
     gene            2401..3462
                     /locus_tag="PWY43_002203"
     CDS             2401..3462
                     /locus_tag="PWY43_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL2137048.1"
                     /translation="MYQIPTLEDVRREILRDIQSLEPTADIEVDSDYFVRASALASCV
                     VGLYAHQNWIVRQYFPDTADSEYLEMHAKLRNLYRKNATYASGSLKVFGTAGSVINEG
                     LQVKFGERFYVTKSSDVIGDDGSATVRVISMATGASSNVKSESKAQLTAAPAGVSSDC
                     LLLPDVTGGTEVESDATLLARLLEQIRRPPSGGNKNDFRQWAESVDGVTSAFVYPLRR
                     GEGTVDIAITSGDTVPSDEIVRATQAYIDEVRPVTAKSVYVLKPVEKRVNFNIKVQLD
                     TDTTLDGVKAEIENALENYFLSLKPADTLIISQIEAVISDLVGVVDREIVQPTRNQTI
                     DAYREMGWFRLGDVTVELM"
     gene            3462..4028
                     /locus_tag="PWY43_002204"
     CDS             3462..4028
                     /locus_tag="PWY43_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDL2137049.1"
                     /translation="MVTINSKHGKALGGLLPPVSYDPNGRWLALSLEVEGRELDRIDA
                     RAKTLIDAVDVSTGIFIDDWERVCGLTANSSLLLETRIERVIAKLNQLGGLSISYLME
                     QAKALGYEFKIHEPEPFRCGINRCGDRLWDKDVIWMFFVDFNRAENTDPYLPAPVIKT
                     PQALKLLLDEIKPAFTRYWVRDFTAWNN"
     gene            4029..>4503
                     /locus_tag="PWY43_002205"
     CDS             4029..>4503
                     /locus_tag="PWY43_002205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2137050.1"
                     /translation="MRQTMNPITTDNGRFKDGNPATGEYGTVVTAEHMNNVQDSVISM
                     QNEIITVLNEAGIAVNPNDNTQLWQALQMIAGQVESIADLRQFEPVKDRQVVFVKAYH
                     TGSDKGGGYFVSDFADTVSADNSVTLFVTANGKRWKRLYSTLNIFDFGVRLDSVNN"
BASE COUNT         1318 a          845 c         1139 g         1201 t
ORIGIN      
        1 gtaatggata gcggcacaat ccataaagta gcccattatt tttatgggga ttacgagcgg
       61 gcggatgagc tgttacgcct taatccgcat attcgccagc cgaactttat tgagcgaggc
      121 acgctgttaa attgttattg ggagtaaaaa tggaaaatga aattgtggtg gagattgacg
      181 gctcgcagca taaaaactgg aaaagttatg atattgacag cgatttttta attcctgctg
      241 atagttttaa ttttgagttg ggtaaatcta gcaatataga ggttttacct aattattcgg
      301 gcaaaacggc aatagttaaa atcaatggca aaacggtgct cactggcatt gtggatacca
      361 cacatcacac cattagcaaa agcgggcgtg ggtttagcct aaatggacgt gataaagccg
      421 ctattttggt ggattgctct gccccgatta ccaatgtgca agggctctct ttgcttgatg
      481 ctgttaaaaa aattgtaacc cctcttggga ttaaagaggt gcaactgaaa gccgagaaaa
      541 acccgacctt aagcaaggta gatattgatg tgggcgagtc agcgtgggaa gcgattatgc
      601 ggtgtgccaa ctcggcaggt ttgcattgtt ggtttgaccc caaaggcgtg ttaattgtcg
      661 gcggtgcgga ttacgctcaa ccgcccgttg ccacgttata ttgcgtgaaa gagtcggggg
      721 atagccgtaa aaataacttt gttgatgcaa cactttctta tgatgtgtcg caaagctatt
      781 cggaggtcac ttttcttgct caaaaacacg gcaaagattc tgataatgcc aagcacgatt
      841 ttaaatgggt gtataaaaat gaggaaatgg agctttataa accgaaaact atcgtgctgg
      901 gcgatgtgga gaacctcgaa gccttgatga aacaggcgaa aaaacaggta tcagattggc
      961 agttggagtc atttaacctc acgattattg tacctgacca taaaaccaaa gacggcacgt
     1021 tgtggcaagc tgggcaacgt gtgcacgtgg tatgtgagga gtatgattta gatggtattt
     1081 tctttttaat ggggcgacgt tttcgactct ctcgcacggg tggcacaacc accgaattgc
     1141 ggttaaaact ggatggcgtg tggacacctg atgcttatca agctaaagca caagaggctc
     1201 gcaagcgtaa aggcaaaaaa ggacgtaaga aaaaagagcc tgtagaatat gtaggctcgt
     1261 gggagttggg aaaataatat gatgagaaaa ttagcaaaac agactaaaga ggtggcaaaa
     1321 ggtgtgcaag atagcgtgcg tgctgctttt agaggggtat taaatttagt caagagtggc
     1381 agtgatattc agcaggtaca ggtgtccgct ttagcggacg aaactatcca agatttagag
     1441 ctaatgcagc attttggttt cacttccgtg ccacctgccg gcacgcaagc ggttgttatc
     1501 cccttaggtg gtaaaaccac gcacggtatt attgtggcga ctgaaaacgg atcgttccga
     1561 gtcaaaaatc tcaacgatgg agaggtggca atttacgatt cgagtggctc aaccatcata
     1621 ttaaaaaacg ggcgagtgat tgatattgag tgcgatagct ttaacattaa atgcaagcaa
     1681 tattccgtta ccgcaagcgg tggagcaaat tttaacaccc cgaaactaga aaccgaccag
     1741 cagtttgttt cacaagggca aatcagcggc aatggcggaa tggcggtgca aggtggagaa
     1801 ggggcaagtt ttagcggcaa tgtaaaccaa accagtggta gctttaaaac aagtggggat
     1861 gtgactgcag gtacggtatc ccttaaaaac catactcatc aaagtgatgg gcgaggtaaa
     1921 ccaaactgat gatttcaagc ggttattttt tgcaaaattt ttgcagaatc taaccgcttg
     1981 ttgtttgtgg aagtctctcc ccgttactcc tgtctttaat gtctctacca tagcaatatg
     2041 gacagagaaa tcagcccgct cactcgggac tatacaagtg acaacatagc tacactgcaa
     2101 aatgccgtgt atatcagact aacaacgcca aaaggctcat ggtgggcaga tgggcgtgta
     2161 ggttctttgc tccatcttat tcagcgagaa aaagatttaa gccgcgtagg gcttttagcc
     2221 caacagtacg ccgaagaggc tttgcagccg attattgatg acggacgagc caaaagcatt
     2281 acggtgacac acgagcagcc aaaagacggc tcgttaaatc ttttaatctc cgttattgat
     2341 aatcgtgggg ttaaatttga gtttaaacac cctgtaaaac tggtttaagt ggtttttaaa
     2401 atgtatcaaa tcccaacgct tgaagatgtt agaagagaaa ttctacgaga tattcaatct
     2461 ttagagccaa ctgctgatat tgaggtggat tcggattatt ttgttcgtgc ctccgctttg
     2521 gcaagctgtg tggtggggct gtatgctcac caaaactgga ttgttcgtca gtatttccca
     2581 gatacggcag actccgaata cctcgaaatg cacgctaaat tgcgaaatct ttaccgtaaa
     2641 aatgcgacct atgcgagtgg ttcgttgaaa gtttttggta ctgctggctc agtaatcaat
     2701 gagggcttgc aagttaaatt tggcgagcgt ttttatgtaa ccaaaagctc ggacgttatt
     2761 ggtgatgatg gctctgcaac ggtgcgagtg atttcaatgg caacaggggc atcaagcaat
     2821 gttaaatctg aaagtaaagc acaactgact gctgctcctg ctggcgtatc aagcgattgc
     2881 ttgttattgc ctgatgtaac aggcggaaca gaagtggaaa gtgatgcgac actgcttgct
     2941 cggttattag agcagattcg ccgaccaccg tcaggcggga ataaaaatga cttccgccaa
     3001 tgggcagaaa gcgttgatgg cgtaacctct gcgtttgttt atccattacg gcgtggcgag
     3061 ggtacggtag atattgcaat cacctcaggt gataccgtgc caagtgatga aatcgtgcga
     3121 gcaactcaag cttatattga tgaagtccgc cctgttacgg ctaaatcggt ttatgtactt
     3181 aagccagttg aaaaacgggt caattttaac atcaaagtgc aacttgatac ggatacgacg
     3241 cttgatggtg taaaagcgga aatcgaaaac gctttagaga attatttttt atcactcaaa
     3301 cctgctgaca ccttaattat ttcgcaaatt gaggcagtaa ttagtgattt agtgggcgta
     3361 gtagatcgtg agattgtaca gccaaccaga aaccaaacga ttgatgccta ccgtgaaatg
     3421 ggctggttca gattgggtga tgtaactgtg gagttgatgt gatggtaacg attaactcta
     3481 aacacggcaa ggcattgggc ggtttattac cgcctgtatc gtatgaccca aacggcagat
     3541 ggctagcgtt atcccttgaa gttgagggga gagagctaga ccgcattgat gcgagagcca
     3601 agactttaat cgatgccgtt gatgtatcaa caggcatttt tattgatgat tgggagcggg
     3661 tttgtggctt aacggcgaat agctcactac tgcttgaaac ccgaattgag cgtgtgattg
     3721 cgaagttgaa tcagcttggt gggttgtcga tttcgtactt aatggagcaa gcgaaagcac
     3781 taggctacga atttaaaatc cacgagcctg aaccctttag atgcggtatt aaccgttgtg
     3841 gcgatagact ttgggataaa gatgtgattt ggatgttttt tgtcgatttt aaccgagccg
     3901 aaaacaccga cccttatttg cctgcacctg tgattaaaac gccacaagca ttaaaactat
     3961 tattggacga aattaaacct gcatttaccc gttattgggt aagagatttt acagcgtgga
     4021 ataactaaat gagacaaacg atgaacccta ttacgactga taacggtcga tttaaagacg
     4081 gtaatcccgc aacgggagag tacggcacag ttgtaacagc cgaacatatg aacaatgtgc
     4141 aagactccgt tatctcaatg cagaatgaga ttataactgt attgaatgag gctgggattg
     4201 cagttaatcc taatgataat actcaacttt ggcaggcatt gcagatgatt gctgggcaag
     4261 ttgagagtat tgctgattta cgccaatttg agccagtaaa agaccgccag gttgtatttg
     4321 ttaaagcgta tcatactggc agcgataaag gtggtgggta tttcgtttcg gattttgcag
     4381 atacagtttc tgcagataat agcgtaaccc tatttgtgac agcaaatgga aaacgctgga
     4441 aacgacttta ttcaacactc aatatctttg actttggagt cagattagac agcgtgaata
     4501 acg
//
LOCUS       DANKBV010000158         4459 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.158, whole genome shotgun sequence.
ACCESSION   DANKBV010000158 DANKBV010000000
VERSION     DANKBV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.158"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..559)
                     /locus_tag="PWY43_002206"
     CDS             complement(<1..559)
                     /locus_tag="PWY43_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2137051.1"
                     /translation="MQFINIANEGVKSLSPYQAGKPIEELERELGIQNIIKLASNENP
                     FGFPESAKKAILNQLDDLTRYPDSNGFELKTTIAQKFGVQANQITLGNGSNDLLELFA
                     HTFAGEQDEIIYSQYAFIVYPLVTKAINAVAREILAKNWGHNLDAFLAAITEKTKLIF
                     IANPNNPTGNFLTQAEIEGFLAKVPA"
     gene            831..2150
                     /locus_tag="PWY43_002207"
     CDS             831..2150
                     /locus_tag="PWY43_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL2137052.1"
                     /translation="MNHLLYSVEDKPPFGLSLLLASQHLLAALGGIIAVPLVLGNVLK
                     LSTADTIILVNAALLISGIVTIIQCRGIGPIGIRLPSVMGTSFTFVAAALAIGFSESG
                     IAGIMGASLVGSLVMIIGSFFMPYIRKLFPPVVTGTVIMMIGLSLIPVAVDWFAGGQR
                     TDPHYATPENLIMASFVLIIVVALVQWGKGIFSAAAIVIGMMIGYIVSLALGWVSFNG
                     VKEAQLFALPQPLHFGLEFPISGIIGMSIAYLVTIVESSGNFLALGNATKTEITGKHL
                     RGGVLCDGLGSAFAAIMSTTPFSSFSQNIGVISLTGVASRYVVAFTGALLVLAGLFPV
                     FGALIVSIPLPVLGGAGLMMFAMIIAAGIQMLDNIQRSKRNGLIIAISIGCGLAVTTR
                     PELLDKLPYFFKEVFGSGITVGSLLALILNLVLPEDKITKKENKSDE"
     gene            complement(2320..4263)
                     /gene="ftsH"
                     /locus_tag="PWY43_002208"
     CDS             complement(2320..4263)
                     /gene="ftsH"
                     /locus_tag="PWY43_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL2137053.1"
                     /translation="MVKNILLWVVIAIVMMTAYEGFNSNFSGSSNTVPYSTFLSDIKE
                     NRLKGVDFKQNDDTILVTKNDGATYQTVMPMYDEYLMADLAKTNAVITGQPAERRGLL
                     SQILISWFPMLLLIGFYVFYMRQMQGGGGRGAMGFGKNKAKMLTADEVKTRFSDVAGC
                     DEAKEEVGEVVEFLRDPTKFQKLGGRIPKGILMVGPPGTGKTLLAKAIAGEARVPFFT
                     MAGSDFVEMFVGVGASRVRDLFDQAKKNAPCIIFIDEIDAVGRKRGGAGFSGGHDERE
                     QTLNQMLVEMDGFEGSEGVIIIAATNRADVLDDALTRPGRFDRQVTVDLPNVKGREQI
                     LKVHLKKVPLAEGVDPMQIARGTPGYSGAQLANLVNEAALFAARKNKRVVTMEDFEEA
                     RDKINMGPERRSNTMTEKEIINTAYHEAGHVIVGYLMPEHDPLNKVTIVPRGQALGFA
                     QFLPEGDRVSETFTKLESQLSTLFAGRIAEGLIFGEDKITTGASSDIHRATQIARAMV
                     TQWGFSKELGPIFYQNEDGMGAIKGVSEESQKLIDVEMRKIIDRNYQRAKQTLEENMD
                     ILHAMKDALLKYETLDSKQIDDLMARRPVGEPSGWTDTPTKDDNATPPSGEQAADVSS
                     TLEDNPNKDNNIGDSLADTKAEH"
     gene            complement(4384..>4459)
                     /locus_tag="PWY43_002209"
     CDS             complement(4384..>4459)
                     /locus_tag="PWY43_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200140.1"
                     /note="Specifically methylates the uridine in position
                     2552 of 23S rRNA in the fully assembled 50S ribosomal
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase"
                     /protein_id="HDL2137054.1"
                     /translation="KVRKPEASRDRSREVYIVATGYKG"
BASE COUNT         1215 a          975 c          848 g         1421 t
ORIGIN      
        1 ttgccggcac tttagctaaa aagccctcaa tttccgcttg ggttaaaaaa ttaccggtcg
       61 gattattcgg gttggcaata aaaattaatt tagttttttc ggtaattgca gccaaaaagg
      121 catctaaatt gtgtccccaa tttttagcca gaatttcacg ggcaacggca ttaatcgctt
      181 tcgtcaccaa cggatacaca ataaacgcat actgagaata gataatttca tcttgctcac
      241 cggcgaaggt gtgggcaaac agctctaata aatcgtttga gccattgcct aaggtgattt
      301 gattggcttg tacgccaaat ttttgggcaa tagtggtttt taattcaaag ccgtttgaat
      361 ccggatagcg ggttaaatcg tcaagctgat tcagaatcgc cttttttgca ctttcaggaa
      421 aaccaaacgg gttttcgttc gaggctaatt taatgatatt ttgaatccct aattcacgct
      481 ctagctcctc aatcggcttt cctgcttggt aaggcgaaag ggatttcacg ccctcatttg
      541 cgatgttgat aaattgcata ataacctctg attattctta taaaatataa gttcggattg
      601 tagtaaagaa tgattgcctt taccagtaaa ttttttgtta caactggaaa gaaattggta
      661 caaacattcg gattagcttt gcaaaaagcg taaaaaatct taccgcttgt catttatcag
      721 ttagggaaaa taaaaaatca ctctcttata taaaaacaat tacgcaaacg attgcttatt
      781 aaaaaaactt cagtataatg ccgacatttt ttaactctcg aggaatcact atgaaccatt
      841 tactttattc tgtcgaagac aaaccgcctt tcgggctgag cttattgctt gcctcacaac
      901 atttattagc cgctttaggt ggcattatcg ctgtgccttt agtgctaggg aatgtactca
      961 aactctctac tgcggatact ataatcttag ttaatgctgc acttttaatt tcaggcatcg
     1021 tcactattat tcaatgtcga ggtattggcc caatcgggat tagactaccg agtgtaatgg
     1081 gaactagctt tacgtttgtt gcagcagcat tagcgattgg tttcagtgaa agtggcattg
     1141 ccggcattat gggagcctct ttagttggct ctctagtgat gattatcggc agttttttta
     1201 tgccttatat ccgtaagctt tttccacccg tggtaacagg tactgtcata atgatgattg
     1261 gcctaagttt aattcctgtt gcagtagact ggtttgctgg tggtcagcgt actgatccac
     1321 actatgcaac cccagaaaat cttataatgg ctagttttgt attaattatt gtcgttgctt
     1381 tagtacaatg ggggaaaggt attttttcag ctgctgctat tgtgattggt atgatgatag
     1441 gttatatcgt ttcgctagct ctgggttggg taagttttaa tggcgtgaaa gaagcacaac
     1501 tttttgcgtt accacaacct ctacattttg gcttagaatt tccaatctcc ggtatcatcg
     1561 gaatgtcaat tgcttattta gttaccattg tagaatctag cggaaatttc ttagcactag
     1621 gcaatgcaac taaaactgaa attaccggaa aacacttacg tggaggtgta ttatgtgatg
     1681 gtttaggctc agcatttgcg gcaattatgt ccacaactcc cttttcttct ttctcacaaa
     1741 atattggggt catttcatta accggtgtag ccagtcgcta tgttgtagca tttacaggcg
     1801 cattacttgt actagccggt ttatttcctg tatttggagc attaatcgtg tctatcccgt
     1861 tacctgtgct tggcggtgca ggcttaatga tgtttgccat gattatcgca gccggtatcc
     1921 aaatgttaga taatattcaa cgtagcaaac gaaacggttt aattattgct atctctatcg
     1981 gttgtgggct ggccgtaaca actcgacctg agctattgga taagcttcca tacttcttta
     2041 aagaagtttt tggctcaggt attacggtcg gctccttatt agcacttatt ttgaatttag
     2101 ttttacctga agataaaata accaagaaag aaaataagag tgatgaataa atcaccctaa
     2161 aaaaacaagg acagctcaca atacaacttt gatgctgtcc tttattccca tagagagcat
     2221 atttaaattt aggttaataa aaaaatctgc actctatatt ggtgtttacc tttagagtac
     2281 agatttcttc ttattctaac tttctagcaa acaattagat taatgttccg ctttggtatc
     2341 ggctaacgaa tccccaatgt tattatcttt atttggatta tcctctaaag ttgaacttac
     2401 atccgctgcc tgttcaccac ttggtggagt ggcgttatcg tctttagttg gggtatctgt
     2461 ccaacctgat ggttcaccca ccggacggcg tgccattaaa tcatcaattt gcttgctgtc
     2521 taaggtttcg tatttcaata aggcatcttt cattgcgtgt aagatgtcca tattttcttc
     2581 caaagtttgc ttcgctcgtt gatagttgcg gtcaatgatc ttacgcattt ccacatcaat
     2641 caatttttgc gactcttccg atacgccctt aattgcaccc attccatctt cattttgata
     2701 gaaaatcggg cctaattctt ttgagaagcc ccattgagta accattgctc gtgcaatttg
     2761 ggttgctcga tgaatatcag aggaagcccc tgtggtaatt ttatcttcac caaaaatcaa
     2821 gccctcagca attcgccctg caaatagggt ggaaagttgg ctttccaatt tggtaaatgt
     2881 ttcgcttaca cggtcacctt ccggtaagaa ttgagcaaaa cctaacgcct gaccacgagg
     2941 cacaattgtc actttattca gcggatcgtg ttccggcatt aaatagccaa caataacgtg
     3001 tccagcttcg tggtaagcag tattgatgat ctctttttcc gtcattgtat ttgaacgtcg
     3061 ttccggcccc atattgattt tgtcacgggc ttcttcgaaa tcttccatcg ttaccacacg
     3121 tttattctta cgggcagcaa ataaggcagc ttcgttcacc aagtttgcta attgagcacc
     3181 agagtaaccc ggcgtaccac gagcaatttg catcgggtca actccctctg caagcggtac
     3241 ttttttcaag tgaactttca aaatttgctc acgccctttc acgttcggca aatcgaccgt
     3301 gacttggcgg tcgaaacgcc ccggacgagt taaggcatca tctaacacat cggcacggtt
     3361 ggttgcagcg atgataatca caccttcaga gccttcaaag ccgtccatct caactaacat
     3421 ttggttgagg gtttgttcac gctcatcgtg tccaccgctg aaacctgcac caccacgttt
     3481 acgccctacc gcatcaattt catcaataaa aataatacaa ggggcatttt tcttggcttg
     3541 gtcgaataaa tcacgcaccc gtgaagcacc cacacctaca aacatttcca cgaagtcgga
     3601 acctgccatt gtaaagaatg gcactcgtgc ttcacctgca atcgctttcg ccaataaggt
     3661 tttacccgta cccggagggc ctaccatcaa gatccctttt ggaatgcgcc cacctaattt
     3721 ttgaaattta gttgggtcac gcaaaaactc aacaacttcg cctacttctt ctttggcttc
     3781 atcacagccg gctacatcag aaaatctggt cttaacttcg tctgcggtca acattttggc
     3841 tttatttttc ccaaagccca ttgcaccacg tcctccgcca ccttgcattt gtcgcatata
     3901 aaacacatag aaaccgatta acagcaacat tgggaaccaa gaaatcaaaa tttgcgagag
     3961 caagccacga cgctcagcag gctgaccggt aatcactgca ttggtttttg ctaaatctgc
     4021 cattaagtac tcatcataca tcggcattac ggtttggtag gttgcaccat catttttcgt
     4081 caccaaaatg gtgtcatcat tctgtttgaa atcgacccct tttaaacgat tttctttaat
     4141 atcgcttaaa aaagtcgaat atggcactgt atttgagcta ccactgaaat tagaattaaa
     4201 accttcatag gctgtcatca tcacaatggc aatcaccacc caaagtaaaa tatttttaac
     4261 catatcgttc aaggttaatc ccctatctgc tgaataaaaa actgttcaag attactataa
     4321 aagcggtcga attttccaat ttttttgcaa aatgttaata aaaaatgacc gcttgcttgt
     4381 tttctagcct ttataacctg tcgctacaat ataaacttct cgagaccgat ctcgagacgc
     4441 ctccggtttt ctgactttt
//
LOCUS       DANKBV010000159         4436 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.159, whole genome shotgun sequence.
ACCESSION   DANKBV010000159 DANKBV010000000
VERSION     DANKBV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.159"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(74..580)
                     /locus_tag="PWY43_002210"
     CDS             complement(74..580)
                     /locus_tag="PWY43_002210"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2137055.1"
                     /translation="MRILPRNENYAATENGEIFSFLSNKILKPRPLKNGYLRVQLVDE
                     NGVKDYLIHRLVCETFHGYPDLHVNHKDRDKTNNRPENLEWCTRSENMLHCSRLGGFS
                     KQSERMKKMNIESCSVPVAAITLDGKLFKKYTSMTSAGKDGFSHSKISLCIQGKRKTH
                     KGYIWQKL"
     gene            complement(558..1154)
                     /locus_tag="PWY43_002211"
     CDS             complement(558..1154)
                     /locus_tag="PWY43_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL2137056.1"
                     /translation="MPLSITANPTKAVQLKHQGEALSGGSETLVVHGTQDPIISQNKA
                     HCLGRNNGQENVLFEVKGAEAVRISEIQDKTPTLKARMGTGGNNIPCIALAGNTIGRQ
                     PQNDGNGNVFDESGVSYMLTRTDVHAVSAGTTIRKLTPTECERLQGFPDGWTKITYRG
                     KSAEECPDSPRYKAIGNSMCTNVMKWIGERLSAYLTSQ"
     gene            complement(1075..2394)
                     /locus_tag="PWY43_002212"
     CDS             complement(1075..2394)
                     /locus_tag="PWY43_002212"
                     /inference="COORDINATES: protein motif:HMM:NF024795.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HDL2137057.1"
                     /translation="MNNIIKFTYGSICSGIEAASVAWHDIGTPLWFSEIEPFPCAVLA
                     HRFPDVPNLGDMTALPEKILNRKIPAPDVLVGGTPCFTAGHMVLTDKGYMPIETLSVG
                     DLVVTHKGQLKPILRVGSEIKPVGKLTAVGLPESIVCTPEHPFYAQKWQTINTKRNGK
                     YHRKTIISKPEWIEAHKLEGYQWVSLTDFSVMPYGGFHSKLTDDEVLLIAGYYLGDGW
                     IRRWKGKNKKAVVISVNKEKYRKFSLSFAKLPHHITTENNSVIKITICDTELADFLYS
                     QFGEKAGGKTIPAWCLSHSFRSKIFEGYMITDGSFRNGVYTANSISKSLAYGIAALSQ
                     TLGYISSVSKVTVAPTKEIQGRIVNQNDYYQMRAFLQSTSRKSRLDENRLLRSVQSFE
                     ITGNEVVYNIEVADDNSYILNNAVVHNCQSDKGGTVKASGGGFEWRE"
     gene            complement(2391..3296)
                     /locus_tag="PWY43_002213"
     CDS             complement(2391..3296)
                     /locus_tag="PWY43_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDL2137058.1"
                     /translation="MQFQEHNNRKKADKFAEYITGEALRRYVAEKVKQYARNNVSVFD
                     GAAGSGQLEQFVRPTSFTAVEVQSESCDVLRHNFPTAQIYNQSFFLYPNGEPCDCVIM
                     NPPFSLKFKDLSQAEQAQIQSEFPWKKSGVVDDIFVLKGLANSHQWGFFILFPGVGYR
                     QTEKRFREEVGNQLVELNRIQNAFEDTPIEVLFLVVDKTKTTDDCSRELIDCAGGQIQ
                     QICADKWKIRPDHWESIQPPPPPQEEIKPLELEAFAREQAIERIVGEVRLSKLIAEFF
                     EPELRRTFNAFIDDICVAVQAEKIV"
     gene            complement(3530..4207)
                     /locus_tag="PWY43_002214"
     CDS             complement(3530..4207)
                     /locus_tag="PWY43_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL2137059.1"
                     /translation="MSNQVQFPVFNFNSSAVRVIIDPNQEPWFCGADVCRILGYVNES
                     LTLQKHCKENGVSKRYLTDKMQRQQKAIFINEPNLYRLIIKSRKPEAEKFEAWVFEEV
                     LPQIRKTGKYALQNNQQNLPLAPPPKKYSFDFTEDELQSLIWAWFAFVRGIHTFRYIY
                     PMFQKLGSNIAPEIYGQGFEYSHTAQSAHKILERITKEFDYDPMTNWRVLKHVRGFDP
                     AFKKPTF"
BASE COUNT         1171 a          939 c          921 g         1405 t
ORIGIN      
        1 cccctcattt agagggattg agttaaataa tcgttaagcc tttgtcctat ccaccgcata
       61 caagggacgg ccattataac ttttgccata tgtaaccctt gtgcgttttg cgtttaccct
      121 gaatgcaaag gctgattttt gaatgtgaga aaccatcttt acctgcactt gtcattgagg
      181 tgtatttttt gaataatttc ccgtccagag ttattgcggc aacaggaact gagcaactct
      241 caatattcat ttttttcatc ctctcggact gcttgctaaa acctccaagt cttgagcaat
      301 gcaacatatt ttctgagcgt gtacaccact ctaaattttc aggtcggtta tttgttttgt
      361 ctctgtcctt atgatttaca tgtagatctg gataaccgtg aaaagtttcg cataccagtc
      421 tatggattaa atagtctttt actccattct catctacaag ctgaactctt aaataaccat
      481 tctttaaagg tctaggtttt aagattttgt tgctcaagaa actaaaaatt tctccattct
      541 cggtagctgc atagttttca ttgcgaggta agatacgcac ttaatctctc tcctatccat
      601 ttcattacat ttgtacacat cgaatttcct atcgccttat atcgtgggct atcagggcat
      661 tcttcggcag atttgccacg gtatgtgatt ttcgtccagc cgtctggaaa gccttgtagt
      721 ctctcgcatt cggtgggagt aagttttcga atagttgttc cagcactgac agcgtgaaca
      781 tcagttcggg taagcatgta acttacacca ctctcatcaa atacgttgcc gtttccgtcg
      841 ttttgtggct gtctgccgat agtattaccg gcaagggcaa tacaagggat attatttcca
      901 cccgtcccca ttcttgcttt cagtgttggc gttttgtcct gaatttcgct aattcgtaca
      961 gcttcagcac cttttacttc aaataagacg ttctcttgcc cgttgtttcg acctaaacaa
     1021 tgagctttgt tttggctgat aattggatct tgcgtaccgt gaacaactag cgtctcactc
     1081 ccgccactca aagcctcccc ctgatgcttt aactgtaccg cctttgtcgg attggcagtt
     1141 atggacaacg gcattattta agatgtagct gttgtcatct gccacctcaa tgttataaac
     1201 aacttcattt ccagttatct caaaggattg cacgcttctc aataatctat tttcatcaag
     1261 gcgacttttt cttgatgtcg attggaggaa cgctcgcatt tgataatagt cattctgatt
     1321 gacaattctt ccttggattt cttttgtcgg agcaacagtt actttggaaa cagatgagat
     1381 atatcctaag gtttgggata atgcagcaat gccgtaagca agggatttcg aaatgctatt
     1441 tgcggtataa actccgtttc taaaagagcc atcggtaatc atataccctt caaaaatttt
     1501 acttctgaaa gaatgagata aacaccaagc cggtatagtt tttccgccgg ctttctcacc
     1561 aaattgcgaa taaagaaaat cagcaagttc agtgtcacaa atagtgattt taattaccga
     1621 attattttcg gtagtaatat gatgaggaag tttggcaaaa gaaagagaaa acttccgata
     1681 tttctcttta tttacagaaa taacaacagc ttttttattt ttgcctttcc atcttcttat
     1741 ccagccatca ccaagataat aaccggcaat tagaaggact tcgtcatcag tcagtttgct
     1801 atgaaaacca ccatagggca taactgaaaa atcagttaat gatacccatt gatagccctc
     1861 tagcttatga gcctcaatcc actcaggttt ggaaatgata gttttcctat ggtattttcc
     1921 attccttttg gtgtttatcg tttgccattt ttgagcataa aaaggatgtt caggggtgca
     1981 aacgattgat tcaggtaagc caacagccgt taattttcca actggtttta tttcactgcc
     2041 aacacgcaaa ataggtttta gttgtccttt atgagtaaca actaaatctc cgacagaaag
     2101 tgtttcgatt ggcatatatc ctttatccgt taaaaccata tgcccagcgg taaagcaagg
     2161 cgtaccccca accaacacat caggagcagg aattttacga ttgaggattt tttcaggtag
     2221 ggcggtcata tcgcccaagt tcggcacatc ggggaaacgg tgagcaagca ctgcacacgg
     2281 aaacggctca atctcgctga accataacgg cgtaccgatg tcgtgccacg ctacacttgc
     2341 tgcctcaatg ccggagcaga tagatccata ggtgaatttt attatgttgt tcatacaatc
     2401 ttctccgcct gcaccgcaac gcaaatatca tcaataaatg cgttaaaagt gcggcgtaat
     2461 tctggttcaa aaaattctgc gatcagcttg gataagcgaa cctcgccgac aattcgttca
     2521 atcgcttgct ctcgggcaaa cgcctctaat tcaagcggtt ttatctcttc ttgtggcggt
     2581 ggtggcggtt ggatactttc ccaatgatca ggtctaattt tccatttatc agcacaaatt
     2641 tgttgaattt gcccaccagc acaatcgatt aactcccgag agcaatcatc ggtcgttttg
     2701 gttttgtcta ccaccaagaa taggacttca atcggggtat cctcaaaggc gttttggatg
     2761 cggttgagtt ctacaagttg attgccaact tcttctcgaa agcgtttttc tgtttggcgg
     2821 tagccaacgc cgggaaagag aatgaaaaat ccccattggt gactatttgc cagtcctttt
     2881 aggacgaaaa tatcatcaac caccccagat tttttccacg gaaattcgga ttgaatttgt
     2941 gcttgttcgg cttggcttaa atccttaaat ttaagggaaa atggcggatt cataatcacg
     3001 caatcgcacg gttcgccgtt aggatagaga aagaagctct gattatagat ttgagccgta
     3061 gggaaattgt gccgtaacac atcgcagctt tcggattgga cctctaccgc agtaaaagag
     3121 gttgggcgga caaattgctc caactgccca gaacctgccg ccccgtcaaa aacagaaaca
     3181 ttatttctgg catactgctt gaccttttca gcaacataac ggcgtagagc ttcacctgtg
     3241 atgtattcag cgaatttgtc ggcttttttg cggttattgt gttcttggaa ttgcattttt
     3301 cctccaaaaa ataaccgctt gcggaacaag cggttgggtt aattagcgtt gagctgtaca
     3361 aattttgcca tcgcagtcgt tgcgtaactc tagatggtca gcagcaactg caaaggcggt
     3421 taaaaagagt atgatttttg cgtatttcat cgtctaatcc tcaaaatttg ggtgcaggaa
     3481 accgccgctt gatgtttcgc aagcggtcgg gttttataat tttttgtgat tagaacgttg
     3541 gctttttaaa tgccgggtcg aaacctcgaa cgtgtttgag tactcgccag tttgtcatcg
     3601 ggtcgtaatc aaattctttg gtaatccgtt caagaatttt atgagccgat tgtgcggtgt
     3661 ggctatattc aaaaccctgt ccgtagattt caggtgcgat atttgagccg agtttttgaa
     3721 acatcgggta gatatagcgg aaagtgtgaa tgccacgcac gaaagcgaac caagcccata
     3781 tgaggctttg gagttcatct tcggtaaagt cgaaagagta tttctttggc ggtggtgcaa
     3841 gcggtaggtt ttgctgatta ttttgcaacg catatttacc agtcttgcga atttgtggca
     3901 gaacttcttc aaatacccac gcttcaaact tttccgcttc tggtttgcgt gatttgataa
     3961 tcagtcggta taggtttggt tcgttgatga agatcgcttt ttgttgtctc tgcattttat
     4021 cggtgaggta tcgtttcgat accccatttt ctttacagtg tttttgcaga gtaaggcttt
     4081 catttacata acctaaaatt cggcaaacat ctgcaccgca aaaccacggt tcttgatttg
     4141 gatcgataat gacacgaaca gcagatgaat tgaaattgaa aacagggaat tgaacttgat
     4201 ttgacatttt ttgtaccttt tggatttaag tatttaatcc gatcattaag tgggtgatcg
     4261 gggttcaact taccctccaa aagatgggcg gagcttattc agatgtattg tatatcgctc
     4321 tctcgccccg atcatttcgg attaaaccta aaatttaggt acaaaaaaac cgctttatat
     4381 cggaggcgga aaccgctttt ggaaagtaag tgtggttatc ttagccgaaa gggcgg
//
LOCUS       DANKBV010000160         4389 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.160, whole genome shotgun sequence.
ACCESSION   DANKBV010000160 DANKBV010000000
VERSION     DANKBV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.160"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1254)
                     /locus_tag="PWY43_002215"
     CDS             complement(<1..1254)
                     /locus_tag="PWY43_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2137060.1"
                     /translation="MSKLSDFISIKHGFAFKGEFITTEENANCLITPVNFSIGGGFKS
                     DKFKYYTGEIPEKYILQPNDLIVTMTDLSKQADTLGYPALVPNISGKKMLHNQRIGLV
                     EFLDNELDKEYLYFLMRTKEYRHQILSTATGATVHHTSPSKILDFEFEKPDLQTQKLI
                     AQYLMILEEKIQLNTQTNQTLEAIAQAIFKSWFVDFDPVRAKAQAILDGKTSDEANLS
                     AMAVFSGKAIEDLSQTEYQELWEIADAFPSEFGDEGLPIGWKFNQADNLFDVGIGKTP
                     PRKESEWFSDNANDTEWISIKDMGNQGLFITESSEYLKVEAVDKFNIKRIPENTVILS
                     FKLTVGRVSITTKETTTNEAIAHFKIPSSSNLSSEFLYCYLKNFDFNNLGSTSSIATA
                     VNSKMIKEMEILEPSDLVINHFNEYI"
     gene            complement(1256..2914)
                     /locus_tag="PWY43_002216"
     CDS             complement(1256..2914)
                     /locus_tag="PWY43_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL2137061.1"
                     /translation="MPNNTARPALDNTVSQAFLNDLDQTLWTAADKLRKNLDAANYKH
                     IVLGFIFLKYISDSFTDFQAKLKTQLTTPESELYLDPALFDEQEFSQILAEELEQRDY
                     YAAENIFWVPEQARWDNIKSLSKLNLGDELPWGDKFKGVSRLIDDAFEAIERENPKLK
                     GVLQRIAGFGVPDEMLTGLIDLFSRTNFTQPMHNGEPVHLQAKDILGHVYEYFLGQFA
                     LAEGKKGGQYFTPKSIVTLIVEMLKPYSGRIYDPAMGSGGFFVQADRFIPAHQGNRNA
                     ISVYGQESNPNTRKLAVMNMAIRGIPFDFGDKPEDTLLNPLHIDKKMDVVMANPPFNQ
                     KAWWSESLANDPRWAYGTPPQGNANFAWLQHMIYHLSPKGKMALLLANGSMSSQTSGE
                     GDIRKNIVQADLVEAMIALPNQLFTNTQIPACIWIINKAKARKKEVLFINATQIGYMK
                     DRVLRDFTADDIAKISDTYHNWQKQDGYENIPAFCYCATLDEIAKNDFVLTAGRYVGA
                     AVEEDDGVPFAQKMQELTALLNEQFRQGRALEEKIVANLKGLGF"
     gene            3075..3452
                     /locus_tag="PWY43_002217"
     CDS             3075..3452
                     /locus_tag="PWY43_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDL2137062.1"
                     /translation="MEYQFTHSIHGVVAKCSMDHEAFARWLNTEITGNPKELEAIFVE
                     IEKCRVAFPNHYECIFEGREYSLYFDYDEVMVKANNLDTAFDENEMEDGFQFYNEESI
                     AFCGLDDFEKLLKAYQYFVQTYH"
     gene            3545..4132
                     /gene="yihI"
                     /locus_tag="PWY43_002218"
     CDS             3545..4132
                     /gene="yihI"
                     /locus_tag="PWY43_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDL2137063.1"
                     /translation="MSRTKKTRRITDIMPARKADKPAKPTPKLGGGKKLKLTRYELDV
                     LAREEKRKRKHKGLPTGSRNADPAEQKKAVVKEVKDPRIGSRKKVPLMVEFVNQPEKG
                     RTIKAVPVEPIKPTLSPEQELEQLENNECLNQLLDDLEAGKTLSAEDQKFMNECLDRI
                     DELMTELGIEYEDEEGDNGDALLRQFETMDINKFR"
     gene            4164..>4389
                     /locus_tag="PWY43_002219"
     CDS             4164..>4389
                     /locus_tag="PWY43_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HDL2137064.1"
                     /translation="MVLAALILISLGGYALHLMLKVRAQKKHEQALIEQAKQAQKDRY
                     IRILDSIEVIARAMISEQCDFSEGVLRLKPL"
BASE COUNT         1222 a          814 c          996 g         1357 t
ORIGIN      
        1 aatatattca ttaaagtgat taataactag atctgatggt tctaaaattt ccatctcttt
       61 tatcattttt gaattgactg ccgttgcgat tgatgatgta cttcctagat tattaaaatc
      121 aaaatttttt aagtagcagt ataaaaactc tgagcttaga tttgatgagc taggaatttt
      181 aaaatgagca atagcttcgt tagtagttgt ttcttttgtc gtaattgaaa ctctaccaac
      241 ggttaattta aaactcaaaa ttacagtatt ttcaggaatt cttttaatat taaatttatc
      301 tacggcttca actttgaggt attcgctact ttccgtaata aacaatccct gatttcccat
      361 atctttaata gaaatccatt ctgtatcatt tgcattatca gaaaaccatt cactctcttt
      421 tcttggtggg gttttcccaa tgccaacatc aaataaattg tctgcttgat tgaatttcca
      481 gccaataggt aacccttcat ctccaaactc acttgggaag gcatcggcga tttcccaaag
      541 ctcttggtat tcggtttggc ttaggtcttc aatcgctttg cctgaaaaga ccgccattgc
      601 tgataaattg gcttcatcgc tggttttgcc gtccaaaatc gcttgggctt tggctcgtac
      661 agggtcaaaa tccacaaacc aacttttaaa aatcgcctga gcaatggctt ctaaggtttg
      721 gttggtttgg gtgttgagtt ggattttttc ttccaatatc attaaatatt gagcaattag
      781 tttttgggtt tgcaagtctg gcttctcaaa ctcgaaatct agtattttgc taggggaagt
      841 atgatgaaca gttgcaccag tagctgtact taatatttga tgtctgtatt ctttcgtacg
      901 catcaaaaaa tacaaatact ctttatctag ttcgttatct aaaaattcaa ccaagcctat
      961 cctttggtta tgcaacattt ttttgcctga aatatttggc actaatgctg ggtatcccaa
     1021 tgtatcggct tgcttgctta aatccgtcat tgttacaatt aaatcatttg gctgtaaaat
     1081 gtatttttca ggtatttctc ctgtataata tttgaactta tctgatttaa aaccgccacc
     1141 aatagaaaaa ttaactggcg taatcaaaca gttggcattt tcttctgttg tgatgaattc
     1201 acctttaaat gcaaatccgt gtttaatgga aataaaatcg gatagtttac tcatcttaaa
     1261 accccaaccc ttttaaattt gccacaatct tttcttccaa tgctcgccct tgtctaaatt
     1321 gttcatttaa aagtgcggtt agttcttgca ttttttgagc aaacggtacg ccgtcatctt
     1381 cttcgactgc cgctcctaca tatcgcccag ccgttagcac aaagtcattt ttggcgattt
     1441 cgtccagcgt ggcacaataa caaaacgcag ggatattttc gtagccgtcc tgtttttgcc
     1501 agttgtggta ggtgtcgctg attttggcaa tgtcatcagc ggtaaaatca cgcaagacac
     1561 ggtctttcat atagccgatt tgggtagcgt tgataaacag cacctctttt tttctggctt
     1621 tggctttatt gataatccaa atgcaggcag ggatttgggt gttggtgaat agctgattgg
     1681 gcagggcaat catcgcttcg acaaggtcgg cttgcacgat gtttttgcga atgtcgcctt
     1741 cgcctgaggt ttggctactc attgagccgt tggcgagcag gagtgccatt ttgcctttgg
     1801 gggagaggtg gtaaatcata tgttgcagcc acgcaaagtt ggcgttgcct tgcggcggtg
     1861 tgccgtatgc ccagcgtggg tcgtttgcta ggctttcact ccaccacgct ttttggttaa
     1921 aggggggatt tgccatcaca acatccattt ttttgtcgat gtgcaaaggg tttagtaggg
     1981 tatcttcggg cttgtcgcca aagtcaaagg gaatgccacg aatcgccata ttcatcaccg
     2041 ccagtttgcg ggtgttgggg ttggattctt gcccataaac cgaaatggcg ttgcggttgc
     2101 cttgatgtgc cggaataaag cggtcagcct gcacaaaaaa tccgccgctg cccattgcag
     2161 ggtcataaat ccgccctgaa tagggtttga gcatttcaac aatcagggta acaatggatt
     2221 tgggcgtgaa gtattgaccg ccttttttgc cttcagctag ggcaaattgt ccaagaaagt
     2281 attcatagac gtgccctaaa atgtctttgg cttgcagatg cacaggttcg ccattgtgca
     2341 taggctgggt gaaattggtg cgtgagaaca ggtcaattaa gcctgtgagc atttcatcag
     2401 gcacgccaaa gccggcaatg cgttggagta cgcctttgag tttggggttt tcccgttcga
     2461 tggcttcaaa ggcatcatca atcaagcggc tgacaccttt aaatttgtct ccccaaggca
     2521 attcatcgcc aagattgagt ttgcttaatg atttgatgtt gtcccagcgg gcttgctccg
     2581 gcacccaaaa gatgttttcg gcagcgtagt aatctctctg ttccaactct tcggcaagaa
     2641 tttggctaaa ttcttgttcg tcaaatagtg cagggtcaag atagagttcg ctttcggggg
     2701 tggtaagctg ggtttttagc ttggcttgga aatcggtaaa gctgtcggag atgtatttta
     2761 ggaagataaa gccaagaacg atgtgtttgt agttggcggc atcgaggttt ttacgcagct
     2821 tgtcggcggc tgtccagagg gtttggtcga gatcgtttaa aaaggcttga ctgaccgtat
     2881 tgtcaagagc aggtcgtgca gtgttatttg gcatagtggt tccttttgga tgtatcaata
     2941 ctaaaatgac ctgtatttta gcagatttac ggataatcga ggaactgata acatttaaaa
     3001 attagcgttt tgcaaaattt tccaaaaaaa gaccgcttgt taggctataa tagccgtaat
     3061 agaggagaag gtgtatggaa tatcaattta cccatagtat tcacggggtg gtggcaaaat
     3121 gttcaatgga tcacgaagca tttgcccgct ggttgaatac ggaaattacg ggcaatccga
     3181 aagagctaga agctattttt gttgaaattg aaaaatgtag agtcgctttt cctaatcatt
     3241 atgaatgtat atttgaaggt cgtgagtaca gcctctattt tgattatgat gaggtgatgg
     3301 taaaagcgaa taatttagat accgcttttg acgagaatga aatggaagat ggctttcaat
     3361 tttataatga ggaaagcatt gctttttgtg gcttagatga ttttgaaaaa ctgttaaaag
     3421 cctaccagta ctttgtgcaa acttatcatt gatgggaatg ctgaattaaa acgatactat
     3481 taagggaacc gaattccctg tagaatagtg atttattaaa attttattaa tcgattaaag
     3541 aattatgagc cgtactaaaa aaacacgccg cattactgat attatgccag caagaaaagc
     3601 cgataaacca gcgaagccaa cacctaaatt aggtggcggc aaaaaattga aactcactcg
     3661 ttatgagtta gatgtgttag cccgtgagga aaaacgtaag cgtaaacata aaggcttgcc
     3721 gacaggctca cgcaatgctg atccggcaga gcagaaaaaa gcagtggtga aagaggtgaa
     3781 agatccgcgt atcggtagcc gtaaaaaagt gccgttaatg gtggaatttg tgaaccagcc
     3841 ggaaaaaggg cggacgatta aggctgtgcc ggttgagcca atcaaaccaa cgctttcacc
     3901 ggaacaagag ctggagcagt tagaaaataa cgaatgttta aatcagttgt tagatgattt
     3961 ggaagcgggc aaaaccttat ccgcagaaga tcagaaattt atgaatgagt gtttagatcg
     4021 cattgatgaa ttaatgactg agcttggcat cgaatatgaa gatgaagagg gcgataatgg
     4081 tgatgcgtta ttacgccaat tcgaaacgat ggatattaat aaattccgct aggtagccga
     4141 ttatgatgct aagaatattc ctgatggtgc ttgccgcttt gattttaatt tcgctgggcg
     4201 ggtatgcact gcacttaatg ctgaaagtgc gagctcagaa aaagcacgaa caagcactga
     4261 ttgaacaggc aaagcaagca caaaaagatc gttatattcg gattttagac agcattgaag
     4321 ttattgctcg ggcaatgatt tccgaacagt gcgatttttc ggaaggcgtt ttacgtttaa
     4381 aaccgctat
//
LOCUS       DANKBV010000161         4357 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.161, whole genome shotgun sequence.
ACCESSION   DANKBV010000161 DANKBV010000000
VERSION     DANKBV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.161"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(140..358)
                     /gene="ilvM"
                     /locus_tag="PWY43_002220"
     CDS             complement(140..358)
                     /gene="ilvM"
                     /locus_tag="PWY43_002220"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDL2137065.1"
                     /translation="MEEYQLTLKANKRPETLERILRVIRHRGFEVKCLNVESTDSTFN
                     LNFTLASKRPLSLLSHQLEKLFDIIEIN"
     gene            complement(368..2020)
                     /locus_tag="PWY43_002221"
     CDS             complement(368..2020)
                     /locus_tag="PWY43_002221"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDL2137066.1"
                     /translation="MNGARFITESLKAHGVTTLFGYPGGAIMPTYDAIYDSGLDHLLC
                     RNEQGAAMAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASH
                     LIGTDAFQEADVLGLSLACTKHSFIVQNIEELPEILALAFQIAQSGRPGPVLVDIPRD
                     VQLAQTSALPIVYPKEPADAQDPNKLVVAKQLLATAKRPVLYVGGGVSMADGVQAVRN
                     FVKIANIPSVSTLKGLGTIDPTDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDR
                     VTGKLDTFAPHAKVIHIDIDNAEINKLRQAQVALKGNLIEAVNYLAQPLDIEPWREDV
                     RNLKQQFDFQYQENSGDGNIDAVALLHTLSQRKAKNSIVTTDVGQHQMWSAQHLTYHD
                     PKNYITSAGFGSMGFGLPAAIGAIKARAEDQVILITGDGSIMMNIQELGTLKRADLAV
                     KILLLDNQRLGMVRQWQSLFFHGRHSQTILDDNPDFVLLAKAFDIDGERIEKASEVSD
                     AIDRMLNAKGAYLLHVCIPQEDNVWPLVPPGACNADMLDDEV"
     gene            2362..3864
                     /gene="nrfA"
                     /locus_tag="PWY43_002222"
     CDS             2362..3864
                     /gene="nrfA"
                     /locus_tag="PWY43_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDL2137067.1"
                     /translation="MKFLIKSLAVATISILGCLQTALAEEAKTESLTDKAVKHEKLGV
                     KIESANHLFAEKYPLQYDSWKSTAKSTDRGSALEADPRYVILWAGYAFAKDYNKPRGH
                     FYAVTDVRDILRTGAPKDENDGPQPMACWTCKGPDVPRLIEEKGERGYFDPKWAKYGA
                     EIVNSIGCADCHDTTSEEFKQGKPALRVARPHVLRALNTVGWKFEDLDKHGKRPAVCA
                     NCHVEYYFKNKTDVTFPWDKGVDVDSIEKYYDEINFTDWTHALSKAPMLKTQHPDFEV
                     WSQGTHGKNGVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFEATCKTCHEQSKETLEK
                     RVKEYKHEVKEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKEILMAIRHAQWRWDYSA
                     AGHGNHFHAPDVMLRTIATGIDRAADARAKLGVVLAKHGVTTPVAIPDISTKEKAQLA
                     IGLDIPKEQAAKDEFLKTVVPQWEKEARAKGLLPAEEADKPVAAPKAEAK"
     gene            3883..>4357
                     /locus_tag="PWY43_002223"
     CDS             3883..>4357
                     /locus_tag="PWY43_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDL2137068.1"
                     /translation="MKKLISKAGRAVALIQAVVFAGFFAISSANALMPEQPKPLWQSE
                     TADDAKVAQDARRDPNKYCTQCHESANATGKPFHHAGKHFQKDVKSPNNGEPLTCVSC
                     HGNISESHRKGAKDVMRFNPHGKASNPSLERSVNEQNQVCFACHTAEKLREKFWAH"
BASE COUNT         1370 a          861 c          933 g         1193 t
ORIGIN      
        1 gaatgggaaa ataaagagca ataagtgaaa aaaaagagtt gaaatttgaa gaaatgaata
       61 aaatcaaaag gatagcgaat gataaatttt aagcataaaa aagccgtgta tttttttaca
      121 cggcgaatga aaagtctgat taattgattt caatgatatc aaacagtttt tctaattgat
      181 ggcttaaaag agagagtgga cgtttacttg ctaatgtaaa atttaggtta aaggtgctgt
      241 ctgttgattc tacatttaag catttcacct caaaaccacg gtgacgtata acccgtaaaa
      301 tacgctctaa ggtttcaggg cgtttgttag ctttaagcgt taattgatat tcttccatct
      361 tattctccta tacctcatcg tctaacatat cggcattaca cgcccccggc ggcaccagtg
      421 gccaaacgtt atcttcctgt ggaatacaaa cgtgaagtaa gtaagcccct ttggcattga
      481 gcattctgtc tattgcatct gacacttcgc tcgctttctc aattcgctca ccgtcaatat
      541 caaaggcttt cgccaacaac acaaaatcag ggttatcatc taaaatggtt tggctgtgtc
      601 gcccgtggaa gaacagcgat tgccattggc gaaccatacc taaacgttgg ttatccagta
      661 ataggatttt gaccgctaaa tcagctcttt taagcgtgcc gagttcttga atattcatca
      721 taatcgagcc atcaccggta attaaaataa cctgatcttc cgctcgagct ttgattgccc
      781 caatcgccgc aggcaagcca aaacccatag agccaaaacc ggcagaggtg atatagtttt
      841 tcggatcgtg ataagttaaa tgttgtgccg accacatttg atgctgccct acatcggtag
      901 tgacgatgct gtttttggct ttccgctgtg aaagcgtgtg taataacgcc acggcatcaa
      961 tattgccatc accactattt tcttgatatt gaaagtcaaa ttgctgcttt aaattacgca
     1021 catcttcacg ccatggttca atatctaacg gttgagccaa gtaattcacg gcttcaatca
     1081 aattcccttt taaggcaact tgtgcttgac ggagcttgtt gatttccgca ttgtcgatat
     1141 caatgtgaat cactttagcg tgaggggcaa aagtgtctaa tttgccggta acacggtcat
     1201 caaagcgggc accacaggca atgagtaaat cacactcttg taccgcgtaa tttgctgctt
     1261 ttgtgccgtg cataccgatc attcccatat agagcggatc ggttggatcg atagtgccta
     1321 acccttttaa ggtagaaaca gacggaatat ttgcaatttt tacaaaattt ctgaccgctt
     1381 gtacaccatc agccatacta acgccaccgc cgacataaag taccgggcgt ttggcggtgg
     1441 ctaataattg tttggcaacc actaatttgt tgggatcttg tgcatcggca ggttctttag
     1501 gataaacgat aggcagagcc gaagtttgag ctagttgcac atcacgtgga atatcgacca
     1561 gcaccggacc aggtctgcca ctttgtgcaa tttggaatgc gagggctaaa atttcaggga
     1621 gctcctcaat attctgaaca ataaaactgt gtttagtgca agcgagtgac aaacctagta
     1681 catcagcttc ttggaaggca tcagtgccga ttaaatggct ggcaacttgt ccggtaattg
     1741 ctaccacagg aatagaatca agtgcggcat cgcctaaacc ggtgattaaa ttagttgccc
     1801 cagggccgga ggtcgcaata caaaccccta ctttacctgt tgcacgggca taaccgatag
     1861 ctgccattgc agcaccttgt tcattgcggc aaagtaaatg atctaagcct gaatcataaa
     1921 ttgcatcata ggtaggcata atagccccgc ccggatagcc aaaaagtgtc gttaccccgt
     1981 gagcttttaa actctcggtg ataaatcttg ctccattcat ggatgttgtg tccttaatat
     2041 ttttattatt ttaaaaccag gcaatataga taacataaat tccctgtttt ttaaacttta
     2101 ttttcagtaa aaataaatag gattccatca tttatttgga caaataaaag tcgtttttat
     2161 ttaggtagag aaaggagtta ctacttaaga ggtattttgg caaattttat tgatttaatt
     2221 caataaatct tcattttgat atagtcggct tggtttaaat ttcatattct cgcaactgaa
     2281 tatcagggat tatcttatta attttaatag gaaaaattct tatttttatc taattatttt
     2341 tacctgagtg ggagagccaa aatgaaattt ttaataaaaa gcctagctgt ggcaacaatt
     2401 tcaatattgg ggtgtttaca aactgcttta gctgaagagg cgaaaactga atcattaaca
     2461 gataaagcag ttaagcatga aaaattaggc gtgaaaattg agtctgcaaa ccacctattt
     2521 gcagaaaaat atccgctaca atacgattct tggaagtcaa ctgcaaaatc cacagatcga
     2581 ggaagtgcgt tagaggctga ccctcgttat gttattttgt gggcaggcta tgcttttgcg
     2641 aaagattata ataaaccacg aggtcacttt tatgcagtta ccgatgtgcg tgatattttg
     2701 cgtaccggcg cgcctaaaga tgaaaatgat ggtccacaac ctatggcttg ttggacatgt
     2761 aaaggccctg atgttccacg cttaattgag gagaaaggtg agcgaggata ttttgatcct
     2821 aaatgggcga aatatggtgc tgaaattgta aactcaatag gttgtgcaga ctgccatgac
     2881 acgacatcag aagagtttaa acaaggtaaa cctgcactac gtgttgcacg tcctcacgta
     2941 ttacgtgcat tgaatacggt tggttggaag tttgaggatt tagataaaca tggtaaacgc
     3001 cctgctgtgt gcgctaactg ccatgtggag tactatttca aaaataaaac agatgtaact
     3061 ttcccttggg ataaaggtgt agatgtagat agcattgaaa aatactacga tgaaatcaat
     3121 tttacagact ggactcatgc tttatcgaaa gctccgatgc taaaaacgca gcacccagat
     3181 tttgaagttt ggtcacaagg tacacatggg aaaaatggtg taacctgtat tgattgccat
     3241 atgccaaaag tgaaagacaa agacggcaaa gtttatactg accacaaaat cggtaatccg
     3301 tttgaccaat ttgaagcgac ttgtaaaact tgtcatgaac aaagcaaaga aacattggaa
     3361 aaacgtgtaa aagagtataa acacgaagta aaagaggcaa tgattcgctt agaagatcaa
     3421 ttagtgaaag ctcactttga agcgaaagct gcttgggaag ctggagcaac tcaagaagag
     3481 atgaaagaga tcttaatggc aattcgtcac gctcaatggc gttgggatta ctctgcggca
     3541 ggtcacggta accacttcca cgcacctgat gtaatgttac gtaccatcgc aacaggtatc
     3601 gaccgtgcag cagatgcccg tgctaaatta ggtgtagtgt tagcgaaaca cggtgtaaca
     3661 acgccagtgg cgattccgga catctcaact aaagagaaag cacaacttgc tattggttta
     3721 gatattccta aagagcaagc agcaaaagat gagttcttaa aaactgtggt tcctcaatgg
     3781 gaaaaagaag ctcgtgctaa aggtttatta cccgcagaag aagcagacaa accggtagca
     3841 gcaccaaaag cagaagcaaa ataatgtagc ttgaggtgaa ctatgaaaaa attaatctca
     3901 aaagcaggac gagcagtcgc cctcatacaa gcggtcgtat ttgcaggttt ttttgcaatt
     3961 tccagtgcga atgcgttaat gcctgagcag ccaaaacctc tttggcaatc agaaacggct
     4021 gatgatgcaa aagtagctca agatgcacgc cgtgatccaa ataaatattg tactcaatgt
     4081 cacgaatcgg caaacgcaac aggtaagcct ttccatcacg caggcaaaca tttccaaaaa
     4141 gatgtgaaaa gcccgaataa tggagagcca ctcacttgtg taagttgcca cggtaatatt
     4201 tctgaaagcc accgcaaagg ggcaaaagac gtaatgcgtt ttaacccaca cggtaaagca
     4261 agtaacccat cacttgaacg ttcggtaaat gagcaaaatc aagtctgttt tgcttgccat
     4321 acggcagaga aattgcgtga aaaattctgg gctcacg
//
LOCUS       DANKBV010000162         4285 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.162, whole genome shotgun sequence.
ACCESSION   DANKBV010000162 DANKBV010000000
VERSION     DANKBV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.162"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(24..1313)
                     /locus_tag="PWY43_002224"
     CDS             complement(24..1313)
                     /locus_tag="PWY43_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2137069.1"
                     /translation="MDSIPLSSLFITLAILLVLSAFFSGSETGLMSLNRYKMRHLAEQ
                     GHKGAKLAEKLLARTDVLLSLILICNNLVNIAASAIATMIGMQLSGEAGVAIATGLLT
                     FVMLVFSEILPKTVAAIYPEKVGFFASFVLTPLKKLLSPIVFLMNLIIAGLMKLLRIQ
                     QKDKQGLSSEELRSVVLDAGKFIPAEHREMLVSILDMEKVTVDDIMVPRNDISGIDID
                     DDWKAIMRQLNHAPHARVIIYKGDMDNNIVGILRVREAFRLMLEKNEFTKEMLLRATD
                     EVYFIPEGTPLTTQLMNFKANKERIGLVVDEYGDIKGLVTLEDILEEIVGEFTTSSAP
                     TLEQEVQQQSDGTVIIEGSANLRDLNKLFDWDLPLEEARTFNGLILEHLEKIPDEGME
                     FELYNLKVMILEVSENMVKQAKVEHFSPKIEKEQDSA"
     gene            complement(1437..2234)
                     /gene="ccsA"
                     /locus_tag="PWY43_002225"
     CDS             complement(1437..2234)
                     /gene="ccsA"
                     /locus_tag="PWY43_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDL2137070.1"
                     /translation="MLLPLLSIVAYALALLWVAPTLVNLENAATVNKKPNMMLVFGFG
                     LLATILHFSVLYNELFLIEGQNFTVTNVISLMSFIMAACVTLALLKWKTVWFPLIIVY
                     SFAICSVGVAGFVKGSFIRNVQENAGLLFHLGIALFSYALFFLALIYAFQLKWLDRKL
                     KSKTMFFCQILPPLMTVERHFFTLTLAAQAMLTLTLFSGVVYLHNFFEPEHIHKAIFS
                     FLAWLVYSIQLLGQWKLHWRGKRVLIYSISGMMLLTIGYFGSRLIVN"
     gene            2450..2974
                     /gene="yceD"
                     /locus_tag="PWY43_002226"
     CDS             2450..2974
                     /gene="yceD"
                     /locus_tag="PWY43_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDL2137071.1"
                     /translation="MQKVKLPLTIDPYKDAQRRVDYEGYISGKLLTRLGESVCNVQSD
                     AQVTLSLYVDPQRLTVIKGTAKVDVEFDCQRCGDPFTQTLDCSFVFSPVSNMDQADIL
                     PEIYEPIEVNEFGEVNLLDMIEDEFILELPLVPMHSEEHCEVSVSEQVFGELPEELVQ
                     KPNPFAVLANLKKN"
     gene            2991..3161
                     /gene="rpmF"
                     /locus_tag="PWY43_002227"
     CDS             2991..3161
                     /gene="rpmF"
                     /locus_tag="PWY43_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2137072.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTTAAVSVDKTSGETHFRHHVTA
                     DGYYRGRKVINK"
     gene            3180..4202
                     /gene="plsX"
                     /locus_tag="PWY43_002228"
     CDS             3180..4202
                     /gene="plsX"
                     /locus_tag="PWY43_002228"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL2137073.1"
                     /translation="MVDITLAVDVMGGDFGPRVTIPALEKALKQNPSLSFLLFGDEHQ
                     ITPLLQKHTLLNHPKIQIHHTEQVIEADVPFTTAIRQSKNSSMRLAIEAVKEGKAQGC
                     VSGGNTGALMGLAKLLIEPLPNIERPALTSLIPTLNGKSSVMLDLGANIEVSNHQLQQ
                     FAEMGNLFAQVMLDLVYPRLSLLNIGTEENKGTAQLQAVHQLLKNRQDLNYTGFIESD
                     KLTSYLTDVIICDGYTGNIALKALEGAAKNIISLFKKEKADSNICRNTKRYLLKAMFY
                     RYYRKLQQINPDRHNGATLLGLSKVVVKSHGGANINAFYYAIDYAVQQIQGDIPNKIL
                     EGLTQK"
BASE COUNT         1389 a          910 c          781 g         1205 t
ORIGIN      
        1 atttactttc ttttttctta aaattaggct gaatcttgct ctttttcaat tttcggtgaa
       61 aaatgttcaa ctttcgcttg tttaaccata ttttcactca cttcaagaat catcactttc
      121 aagttgtaga gttcaaattc cattccttca tcaggaattt tttctaaatg ctctaaaatc
      181 aagccgttaa aggttctggc ttcctcaagc ggtaaatccc aatcaaatag tttgttcaga
      241 tcacgcaggt tggctgagcc ttcaataatc accgtaccat ctgattgttg ctgaacttct
      301 tgctcaaggg tcggtgctga tgaagtcgta aattcaccca caatttcttc taaaatatct
      361 tcaagagtga ctagcccttt aatatcacca tactcatcaa ccactaaacc aatacgctct
      421 ttattcgctt taaagttcat taattgtgta gtgagtggtg tgccttcagg aataaaatag
      481 acttcatctg ttgcacgcag tagcatttct ttggtaaatt cgtttttctc caacattaaa
      541 cgaaaggctt cacgcacccg caaaatccca acaatattgt tgtccatatc ccctttgtag
      601 ataatcaccc gagcgtgagg ggcgtgatta agctgacgca taatcgcttt ccagtcatca
      661 tcaatatcaa tgccgctgat gtcgttgcga ggcaccataa tgtcatcaac cgtcactttt
      721 tccatatcta aaatagagac caacatttca cggtgttcgg caggaataaa tttaccggca
      781 tcaagcacta cactacgcag ctcttcggag cttaagcctt gtttatcttt ttgttgaatc
      841 cgcaataatt tcatcagccc ggcaataatc aaattcatta aaaatacaat cgggctgagt
      901 aattttttaa gcggtgttag cacaaagctg gcgaaaaaac cgactttttc agggtaaatt
      961 gccgctactg ttttaggtag gatttccgaa aaaaccaaca ttacaaaagt tagcaaaccg
     1021 gttgcaattg ccacaccggc ttcaccggat aactgcatac cgatcatcgt tgcaatcgca
     1081 gaggcggcaa tgttgactaa gttattacaa attaagataa ggctaagtag cacatcggtg
     1141 cgtgccaaga gtttttccgc cagtttcgcc cctttatgcc cttgctcggc aaggtgacgc
     1201 attttgtagc ggtttaaaga catcaagccg gtttccgagc ctgaaaaaaa tgcagagagt
     1261 actaaaagaa tagctaaggt aataaataaa ctactcaggg gaatactgtc caaaattcaa
     1321 ttcctatgta aattaatcat aaaaggtagc gtaacactaa aagcgttgtc actacctcac
     1381 tttgtcggtt cagccgactt gcaaaaaatt ttgctttttt gaccgcttgt agatcattaa
     1441 ttgacgatca aacgactacc gaaatagccg atggtgagca acatcatacc cgaaatcgaa
     1501 taaataagca cccgttttcc acgccaatgt aatttccact gtccaagtaa ctgaatgcta
     1561 taaacaagcc aagctaaaaa ggagaaaatt gctttatgaa tatgctccgg ctcgaaaaag
     1621 ttatgcaaat aaaccacacc ggaaaataaa gtgagcgtca gcatagcttg tgcagctaag
     1681 gttaaagtaa agaaatggcg ttcaaccgtc attagcggtg gtagaatctg gcaaaagaac
     1741 attgttttgc tctttagttt tctatccagc catttgagtt gaaaagcata aattaatgct
     1801 aaaaagaaga gagcataaga aaataatgca atccctaaat ggaaaagcag tcccgcattt
     1861 tcttgcacat tacggataaa actacctttt acaaatccag ccacccccac actacaaatg
     1921 gcgaaagagt acacaataat taaaggaaac caaactgttt tccattttaa caacgcaagt
     1981 gtgacacaag ccgccataat gaaactcatc aaagagatta catttgttac cgtaaaattc
     2041 tgcccttcga ttaaaaataa ttcattatat aacacagaaa aatgcaaaat cgtggcaagt
     2101 aacccaaacc caaagactaa catcatatta ggctttttat taactgttgc ggcattttct
     2161 aaattcacta gagtaggggc aacccaaagt agcgctagag cgtaagcaac aatggataaa
     2221 agaggaagta acatattgtc tcgaatctcg tcaaataaga ataactatca gaattttaat
     2281 caaaagtaac cgttttagct agtttttgct gttgctagat caaaaattat cctaaaaaat
     2341 gtgcttttcg ccttaaattg aaccctacct tttctcggta agattatctc actgaaaaca
     2401 ataattttcc tttgactaat ttgctttcta tctgtaatat gcacacccta tgcaaaaggt
     2461 aaaactaccc cttacgattg acccttacaa agatgctcag cgtcgcgttg attatgaagg
     2521 ctacatttca ggtaaacttc taacacgctt gggtgaatca gtatgtaatg tgcaaagtga
     2581 tgcacaagtc actctctcgt tatatgtcga tccacaacgc ctaactgtga ttaaaggcac
     2641 agcaaaagta gatgtggaat ttgattgcca acgatgtggt gacccgttta cacaaacgct
     2701 cgactgttca tttgttttca gtccggtgtc taacatggat caggcggaca tcttgcccga
     2761 aatttatgaa cctatcgaag taaacgagtt cggtgaagta aatttactag atatgattga
     2821 agatgaattt attcttgaat tacctctagt cccgatgcat agtgaagaac actgtgaagt
     2881 gtccgtaagt gaacaggtat ttggcgagtt gccggaagaa ctggtacaaa aaccaaaccc
     2941 gttcgcagta ttagctaatt taaagaaaaa ctagatctag gagaaagccc gtggctgttc
     3001 aacaaaataa gaaatctcgt tcacgtcgtg atatgcgtcg ttcacacgat gcattaacaa
     3061 ctgcagcagt ttcagtagat aaaaccagtg gtgaaactca ctttcgccac cacgtaactg
     3121 cagacggtta ctaccgtggt cgtaaagtaa tcaacaagta attcctttcg aggtttatct
     3181 tggttgatat cactcttgca gtagatgtga tgggcgggga ctttggtccc cgcgttacta
     3241 ttcctgcact ggaaaaagcc ttaaaacaaa atccaagcct ttctttttta ttatttggtg
     3301 atgaacacca aataacccct ttattacaaa aacacacact actcaaccac cccaaaatcc
     3361 aaattcatca taccgaacaa gtgattgaag ctgatgttcc gttcactact gcaatccgcc
     3421 aaagcaaaaa cagctctatg cggttagcga ttgaggcagt aaaagagggc aaagcacaag
     3481 gttgtgttag tggcggtaac actggtgcgt taatgggatt ggcaaaatta ttgattgaac
     3541 cattaccaaa tattgaacgc cctgcactca cttcgctaat tcctaccctc aacggcaaat
     3601 ccagcgtaat gttagattta ggggcaaata ttgaagtaag caatcaccag ctacaacaat
     3661 ttgcagaaat gggaaacctg tttgctcaag taatgcttga tttagtttac cctcgcctgt
     3721 ctttattgaa tattggtaca gaagaaaata aaggcacggc acaacttcag gcagtgcatc
     3781 aactgctgaa aaacagacaa gacttaaatt acaccggctt tattgaaagc gataaattaa
     3841 ccagctatct cactgatgtg attatttgtg acggctacac cggcaatatc gcgttaaaag
     3901 cgttggaagg ggcagctaaa aatattattt ctctctttaa aaaagagaaa gccgattcca
     3961 atatttgccg taacaccaaa cgatatttac tgaaagcgat gttctaccgt tactaccgca
     4021 aattgcaaca aattaaccct gaccgccata acggggcgac cttgctcggc ttatctaaag
     4081 tagtcgtgaa aagccacggc ggggcaaata tcaatgcctt ttattacgct attgattatg
     4141 cggtgcaaca aattcaaggc gacattccaa ataaaatcct agagggttta acccaaaaat
     4201 aagcgaactc aactgttcgc ttattttcat ttcttgtttc acttaaagat acaagcggtc
     4261 aaatttgcaa aaaaatttac ccatt
//
LOCUS       DANKBV010000163         4269 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.163, whole genome shotgun sequence.
ACCESSION   DANKBV010000163 DANKBV010000000
VERSION     DANKBV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.163"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            24..1220
                     /locus_tag="PWY43_002229"
     CDS             24..1220
                     /locus_tag="PWY43_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2137074.1"
                     /translation="MLNTQFRTHFPFFEKSDGWTYLDSAATTLKPDVLIEATSEFYAS
                     AGSVHRSQYDLPQTEQYELARDLVAKRFNVETREAVIWTSGTTQSINTVAYGLEHQIA
                     AGDEIVISVAEHHANFIPWQQLAQRTRAKLVVLPLNDKYEIELETLKNALSEKTKIVA
                     FNLVSNVTGVRQPAEQIIPLIRQSCSAKIVLDIAQAVCSEQVDVQKLDADFYAFSAHK
                     MYGPNGVGVLTGKLQSLEQIQPLVFGGKMLKNITESELTVAELPYRLEAGTPNIAGII
                     GFGQVLAWLEKWDFVDLNHQLYDLAEYTCKRLNSYQNLQIIGQQNTPTISFVVKNQHP
                     ADIAAFLTQSKIAIRQGEHCAKPYLRHLEEVGTVRLSLAHYNNQQDVEHFFNALDFTL
                     EILQES"
     gene            1327..2301
                     /gene="pfkA"
                     /locus_tag="PWY43_002230"
     CDS             1327..2301
                     /gene="pfkA"
                     /locus_tag="PWY43_002230"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL2137075.1"
                     /translation="MSKKIKKIAVLTSGGDAPGMNAAIRGVVRTALNEGLEVCGIQDG
                     YYGLYHDKVIPLDRRSVSETINRGGTFLGSARFPDFKRPEVRKHCVETLKKYSIDALV
                     VIGGDGSYMGAKLLTEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETALDAIDRLRD
                     TSSSHQRISIVEIMGRHCGDLTLSAALAGGCEYVIVPEKGLDKDALMRSIEEGFHKGK
                     RHSIIAITELMTDVHALAKEIEARFGNETRATVLGHIQRGGSPCAFDRILASRMGVYA
                     VELLLQGFGGHCVGIQNEQLVHHDIIDAINNMRRPFKEELFEASRKLF"
     gene            2369..3076
                     /locus_tag="PWY43_002231"
     CDS             2369..3076
                     /locus_tag="PWY43_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HDL2137076.1"
                     /translation="MFKEKPVISNQHGALAMAMLPFLYAVIESGFSTLHIFFGLSWLF
                     LYFFSYPFLSLFSKKPTERNKKWAKIYFGLSILFALPLIYAQPSILQFLPIILPLGLV
                     QVHYAKQKDERNLWNDIAGILTFGIMGMAAYYLVTKQYQFEILLHPTLFFIATTFYIK
                     SMVRERRNPIYMEISIAIHLVLAMLYILCSLQAVFFAYLIATARAIIVPSFGWNVKQV
                     GMLEFPVILLFLASLIW"
     gene            complement(3121..>4269)
                     /gene="thiI"
                     /locus_tag="PWY43_002232"
     CDS             complement(3121..>4269)
                     /gene="thiI"
                     /locus_tag="PWY43_002232"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2137077.1"
                     /translation="LEGKTFSVRVRRKGKHDFRSLDVERYVGGGLNQRIETAKVKLTN
                     PDVTVRIDIEHDKMLLIKARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSR
                     VHYCFFNLGGAAHEIGVKQMAYHIWSRYSTSHKVRFIAINFENVVGEILEKVDNGQMG
                     VVLKRMMVRAASRIAERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITH
                     DKEQIIAMAKAIGTDDIAKSMPEFCGVISKNPTVKAIETKILEEEGNFNFEVLEQAVQ
                     TASYLDIREIAQQTEKEVVSVETTTELSKNDVVLDIRSPEEVDEKPFRLDGVEVKELP
                     FYKLSSQFSTLDQSKTYLLYCQRGVMSKLQALYLKENGYSNVKVFRVK"
BASE COUNT         1212 a          928 c          848 g         1281 t
ORIGIN      
        1 ccacaactct taggaattaa cttatgctta acacccaatt tcgcacccat ttcccttttt
       61 ttgaaaaatc tgacggatgg acgtatttag attctgccgc tacaacgctg aaacctgatg
      121 tactcattga agcaacaagt gagttttacg cttctgcggg gtcggtgcat cgcagtcagt
      181 acgatttgcc acaaaccgaa caatatgaat tagctcgaga tttagtcgca aaacgtttca
      241 atgtggaaac tcgtgaagcg gtgatttgga caagcggtac aacccaaagt attaacaccg
      301 tggcttatgg cttggagcat caaattgcag caggcgatga aattgtaatt tcagtggcgg
      361 aacatcacgc taattttatt ccttggcagc agttggcaca gcgtacaagg gcgaaactcg
      421 tggtgttgcc acttaatgat aagtatgaaa ttgagcttga aacccttaaa aatgcgttat
      481 cggaaaaaac caaaattgtt gcctttaatt tagtttctaa cgtaacagga gttcgccagc
      541 cagcggaaca aataatcccg cttatccgcc aatcttgctc tgctaaaatt gtgttagata
      601 ttgctcaagc agtttgctca gagcaagtgg acgttcagaa attagatgca gatttctacg
      661 ctttttccgc ccataaaatg tatggtccaa acggtgtggg cgtgcttacc ggcaaattgc
      721 aaagtttgga gcaaattcaa ccgcttgtat ttggcggcaa aatgctgaaa aacattacgg
      781 aaagtgaatt aactgtggca gagttacctt atcgcttgga agctggcacg ccgaatatcg
      841 ccggcattat cggctttggg caagtgctag cttggctcga aaaatgggat tttgtcgatc
      901 ttaatcatca gctttatgac ttggctgaat acacctgcaa gcggttaaat tcttatcaaa
      961 atttgcaaat tatcgggcaa caaaatacac cgactattag ctttgtggtg aaaaatcagc
     1021 accctgccga tattgccgct ttcttaaccc aaagtaaaat tgcgatccgc caaggggagc
     1081 attgtgccaa gccttattta cgccatttag aagaggtcgg aacagttcga ctttcacttg
     1141 cccattacaa taatcaacaa gatgttgagc attttttcaa cgctctagat tttacccttg
     1201 aaattttgca agaaagctaa gctaaatcaa aaaaatacgt attttttagt agaacgaaaa
     1261 ggctgaatat tgtaaccttg caccagtttc caatttaact ttaatttatc aacaaggtgt
     1321 ttttaaatga gtaaaaaaat caaaaaaatt gcagtattaa ccagtggtgg tgatgcacca
     1381 ggaatgaacg cggcaatccg tggtgtggtt cgtaccgcac ttaatgaagg tttagaggtt
     1441 tgtggtattc aagacggcta ctatgggctt taccacgata aagtgatccc acttgaccgc
     1501 cgttccgtgt ctgaaaccat caaccgaggc ggtacattct taggttctgc ccgcttccct
     1561 gattttaaaa gaccagaagt tcgcaaacat tgtgtcgaaa ccttaaaaaa atacagcatt
     1621 gatgcattag tggttattgg tggcgacggt tcttatatgg gggctaaatt attaaccgaa
     1681 gaatttggtt acccttgtat cggtattccg ggtacgattg ataacgatat tgtgggaacg
     1741 gattacacca tcggctatca aactgccctt gaaaccgcat tagatgcgat tgaccgcctg
     1801 cgtgatactt caagttcaca ccaacgtatt tcgattgtgg aaattatggg tcgccactgt
     1861 ggtgatttaa ccctaagtgc tgccctcgcc ggtggttgcg aatatgtgat cgtaccggaa
     1921 aaaggcttag ataaagatgc attaatgcga agcattgaag aaggtttcca caaaggtaaa
     1981 cgccattcaa ttattgcaat tacagaattg atgaccgatg ttcacgcatt agcgaaagaa
     2041 attgaagcac gtttcggcaa tgaaacccgt gcaaccgtat taggtcatat tcaacgtggt
     2101 ggttcgcctt gtgccttcga ccgtattttg gcctcccgta tgggcgtcta tgcggtggaa
     2161 ttattactgc aaggctttgg cggtcactgt gtcggtattc aaaacgagca gttggttcac
     2221 cacgatatta ttgatgcgat caataatatg cgtcgtccgt tcaaagaaga attatttgaa
     2281 gcctctagaa aattattcta acttttctta caatttaaag gcggctgttt agtcgccttt
     2341 ttatttgcca cattaagagg gaaattaaat gttcaaagaa aaacccgtta tatccaacca
     2401 acacggtgca ttagcaatgg caatgttgcc gtttctctac gccgttattg aaagtggttt
     2461 tagcactttg catattttct ttgggctgtc gtggctgttt ctctacttct tttcctaccc
     2521 ttttctctca cttttcagta aaaagccaac tgaacgcaat aaaaaatggg cgaaaatcta
     2581 ctttggctta agcattctat ttgcattacc tttgatctac gctcagcctt cgattttgca
     2641 atttttaccg ataattctac cgcttgggtt agtgcaagtt cactacgcca aacaaaaaga
     2701 tgaacgcaat ttatggaatg atattgccgg cattctaacc tttggcataa tgggaatggc
     2761 agcttattat ctggtaacga aacaatatca gtttgagatt ctgctccacc ctaccctatt
     2821 ttttattgct acgactttct atataaagag tatggtgcga gagcgaagaa acccaattta
     2881 tatggaaatc agtattgcaa tacatttggt tttagcaatg ctttacatac tctgctctct
     2941 acaagcggtc ttttttgctt atttaattgc aactgcccga gccattattg tgccaagctt
     3001 tggctggaat gttaaacaag ttggaatgct tgaatttcct gtgatcttac tctttttagc
     3061 aagccttatt tggtaaaaaa ttgcacctta atcaatcatt aaggtgcaat caaagaggca
     3121 ttatttcact cgaaacactt ttacattgct gtagccattc tctttcaaat aaagagcctg
     3181 cagtttactc attaccccac gttggcaata gagcaagtag gttttgcttt ggtcaagcgt
     3241 actaaactgg ctggaaagtt tataaaacgg gagttctttt acttcaaccc cgtctaaacg
     3301 gaatggtttt tcatcaacct cttccgggct gcgaatatct agcacaacat cgtttttact
     3361 caattctgtt gtggtttcaa ccgaaacaac ctctttttcg gtttgctgag cgatctcacg
     3421 aatatctaaa taactcgccg tttgaaccgc ttgttctagt acctcaaaat taaagttgcc
     3481 ttcttcttcc aaaattttag tttcgattgc cttaaccgtc ggatttttag aaattactcc
     3541 acagaactcc ggcattgatt tagcaatatc gtcagtgcct atcgcttttg ccattgcaat
     3601 aatctgctct ttatcgtgag tgatgagcgg acgtaatacc agcgtatctg atgccttatc
     3661 aattaaacgt aaattagtca gtgtttggct agagacctgc cccaacgcct caccggtcac
     3721 aatcgcttga atatcaaaac gctcggcaat acggcttgcc gctcgcacca tcatacgttt
     3781 taataccacg cccatttgtc cgttatcaac tttttctaaa atctcaccca ctacattttc
     3841 aaaattaatg gcgataaaac ggactttgtg cgaggtacta taacggctcc aaatatgata
     3901 tgccatctgt ttcacgccaa tttcgtgtgc ggcaccacct aaattgaaaa aacagtaatg
     3961 cacacgagaa cctctgcgaa tcagcatata gctcgataca cccgaatcaa aaccacccga
     4021 aatcaatgac aacacatctt cttgtgtgcc gattggatac ccgcctaacc cttcgtggcg
     4081 agctttaatc agcaacattt tatcatgttc aatatcaatt ctgaccgtaa catctgggtt
     4141 ggttaatttc actttcgccg tttcaatccg ctgattcagc ccaccaccga cataacgctc
     4201 tacatcaaga gaacggaaat cgtgctttcc tttacggcga acccgcacgc taaacgtttt
     4261 gccttctag
//
LOCUS       DANKBV010000164         4182 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.164, whole genome shotgun sequence.
ACCESSION   DANKBV010000164 DANKBV010000000
VERSION     DANKBV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.164"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..328)
                     /locus_tag="PWY43_002233"
     CDS             complement(<1..328)
                     /locus_tag="PWY43_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596212.1"
                     /note="membrane spanning protein in TolA-TolQ-TolR
                     complex; involved in the tonB-independent uptake of group
                     A colicins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system subunit TolQ"
                     /protein_id="HDL2137078.1"
                     /translation="MQTEFSLLSLFLEASIVVKIVMLILIVFSILSWAVIIQRSKILT
                     KAKKDSLAFEDRFWSGEDLNRLHQGLENRRDALNGSEQIFYVGFKEFTRLQQANPDAP
                     ESIIQGS"
     gene            complement(350..751)
                     /gene="ybgC"
                     /locus_tag="PWY43_002234"
     CDS             complement(350..751)
                     /gene="ybgC"
                     /locus_tag="PWY43_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDL2137079.1"
                     /translation="MDFPIRVYYEDTDAGGVVYHSNYLNFFERARTEFLRQQHFSQQE
                     LFSQSLAFVVKRIEIDYKLPARLDDLLNVETTLSELKKATLIFKQRLWKDGDCLSEAN
                     VTVACVDLIKMKPVAIPDDIRQALQKSYFSI"
     gene            complement(914..1201)
                     /gene="ybgE"
                     /locus_tag="PWY43_002235"
     CDS             complement(914..1201)
                     /gene="ybgE"
                     /locus_tag="PWY43_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDL2137080.1"
                     /translation="MIHKLYNLTAKGLLKALSFILAAVLFATIWVNSTAFALSFGGKT
                     PYLAMLVFYGMAILWVHGIGFEIRAAIWKVIFLPLLGYLIVIPALWILLVK"
     gene            complement(1201..1299)
                     /locus_tag="PWY43_002236"
     CDS             complement(1201..1299)
                     /locus_tag="PWY43_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CydX/CbdX family cytochrome bd oxidase small
                     subunit"
                     /protein_id="HDL2137081.1"
                     /translation="MFYVTWVLGVLLAIFFATVITISIEKTGKFDE"
     gene            complement(1314..2447)
                     /gene="cydB"
                     /locus_tag="PWY43_002237"
     CDS             complement(1314..2447)
                     /gene="cydB"
                     /locus_tag="PWY43_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDL2137082.1"
                     /translation="MIDYEILRFIWWILIAVLLIGFAITDGFDMGVLNLLPIIGKSNV
                     ERRIMINTIAPHWDGNQVWLLTAGGAIFAAWPTVYATSFSGFYIAMVLVLAALFFRPV
                     GFEYRAKIDNPKWRSAWDWGLFIGGFVPSLVFGVAFGNLLQGVPFEFNNINQSQYTGS
                     FFGLLNPFALVCGIISLMMLTTQGATWLQMKTTDELHNNSRKVAQVTASIVLVAFILA
                     GVWLYFKDGFVITSELDHNAQSLLTNKTVAVQAGAWFRNYAEMPVLWVVPALVVVGAL
                     LTIIASKANRSGLAFFSSSLMIVGVILTAAVSMFPFIMPSITHPEMSLTVWDATASHT
                     TLTVMLFVAGIFVPLVLAYTIWSYIKMYGRLDASYLEENKAAY"
     gene            complement(2460..4010)
                     /locus_tag="PWY43_002238"
     CDS             complement(2460..4010)
                     /locus_tag="PWY43_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL2137083.1"
                     /translation="MLDVVELSRLQFALTALYHFLFVPLTLGLSFVLVVMETLYVTTG
                     KEVYKDMTKFWGKLFGINFALGVTTGITMEFQFGMNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWERLSKAKHLLATYCVAFGSNFSALWILVANGWMQNPIASEFN
                     FETMRMELSSFSELVLNPVTQSKFLHTVTAGYVCGAIFVLGISSYYILKGRDLGFAKR
                     SFSVGASFGLIAIISVLIMGDESGYEIGKAQPTKLAAMEGEFETQPAPAAWNAFVIPN
                     TAEMKNEFSIHIPYAAGIIATRSLDKEIKGLKDIRVINEQRVRTGMVAYGLLEKLRSG
                     NYTEADKEAFKAVQKDLGFGLLLKQYTPNVVDATEEQIQKAADNTIPNVGPTFWAFRV
                     MMLSGFLMLILITTAFIQNMRGKVESSRFLLRGLLFGLPLPWIAIESGWFLAEYGRQP
                     WAIYEMLPVGVSNSALTTGDLWFTIGLLCGLYTLFLVVEMYLMFKYGRLGPSALKTGR
                     YYFEQSTK"
BASE COUNT         1493 a          839 c          661 g         1189 t
ORIGIN      
        1 tagaaccttg aataatcgat tctggcgcat ctgggttcgc ttgttgtaaa cgggtaaatt
       61 ctttaaaccc aacataaaag atttgctctg aaccatttaa tgcatcacga cgattctcta
      121 aaccttggtg taaacggttt agatcttccc ctgaccagaa tcgatcttca aaagcaaggg
      181 aatctttttt ggctttagtt aaaattttgc tgcgctgaat aatcaccgcc caagacaaaa
      241 ttgagaatac aataaggatc agcatcacga tctttaccac aatacttgct tctaaaaata
      301 acgaaagaag actaaattca gtttgcatta ataaaactcc gaatagtttt taaatagaaa
      361 aataactttt ttgtaatgct tgacgaatat catcaggaat tgcaaccggc ttcattttga
      421 ttaaatcaac acaagcgacc gtaacatttg cctcactcag acaatcgcca tctttccaca
      481 atcgttgttt aaagataagg gttgctttct tcaattctga gagtgttgtt tccacattta
      541 ataaatcatc taaacgagca gggagcttat aatctatttc aatcctctta acaacaaaag
      601 caagggattg gctaaaaagt tcctgctgtg agaaatgctg ctgtctcaaa aattccgttc
      661 ttgcccgctc aaaaaaattc agatagttgg aatgataaac tactccgccg gcatcagtat
      721 cttcataata aacacgaatc gggaaatcca taagcattat ttctctcatt caaaatgaaa
      781 aaacgcccaa aatttaggcg caaattgtga gatattttag gatcagaaat attgttatgc
      841 aagtatattt tttggtaaat tgcaaaaata ttttttatct atcaaaaata aaaatagaga
      901 cataaaaatg cctctattta acaagtaaaa tccataatgc cggaataaca ataaggtaac
      961 ctagaagtgg taaaaaaatc actttccaaa tagcagctcg gatttcaaat ccaattccat
     1021 gtacccataa aatagccata ccataaaata ccaacattgc taaatacggc gtttttccac
     1081 caaaagaaag agcgaaagcg gtcgaattta cccaaatagt tgcaaataat acagccgcta
     1141 aaataaaaga aagggctttt aacaagccct ttgctgttaa gttatagagt ttatgaatca
     1201 ttactcgtca aacttacctg ttttttcaat gctgatcgtt atgactgttg caaagaagat
     1261 tgcaagcaat acacctaaga cccaagttac ataaaacata ttgtgtctcc ttattagtaa
     1321 gctgctttat tttcttctaa ataactagca tctaaacgac catacatttt gatatatgac
     1381 caaatagtat atgctaatac taacggtaca aagataccag caacaaataa cattacagtt
     1441 aatgttgtgt ggctagcagt agcgtcccaa accgttaagc tcatttctgg atgagtaatt
     1501 gacggcataa tgaatgggaa cattgatact gcagcagtta aaatcacccc aacaatcatc
     1561 aatgatgatg agaagaatgc taaacctgaa cgatttgctt ttgaggcaat aatagtcagt
     1621 aatgcaccta ctacaactaa tgcaggaaca acccataata caggcatttc agcataattt
     1681 ctaaaccatg caccagcttg aaccgctaca gttttattcg tcaatagaga ttgtgcatta
     1741 tgatctaact cgcttgtgat cacaaaacca tctttaaaat ataaccatac acctgcaaga
     1801 ataaatgcca ctaatacaat agaagctgtc acttgtgcca cttttcttga gttgttatgt
     1861 agctcatcag tagttttcat ttgtaaccaa gttgcaccct gtgtggttaa catcattaag
     1921 ctgataatac cgcacactaa tgcaaatgga tttaataaac caaagaatga acccgtatat
     1981 tgtgattggt taatattatt aaattcaaat ggtacacctt gtaataagtt accgaatgca
     2041 acaccgaata ctaatgatgg aacgaaacca ccaataaata atccccaatc ccaagcacta
     2101 cgccatttcg gattatcaat ttttgcacga tactcaaaac ctacagggcg gaaaaataat
     2161 gctgcaagca caagcaccat tgcaatatag aaacccgaga acgaagttgc atagactgtt
     2221 ggccatgcag cgaagatcgc accaccagcg gtcaataacc atacttggtt accatcccag
     2281 tgaggggcaa ttgtgttgat cataatacga cgttcaacat tacttttacc aatgataggt
     2341 aaaaggttta atacgcccat atcaaaacca tcagtaattg caaagccaat taacaatacc
     2401 gcaattaaga tccaccaaat aaagcggaga atttcataat cgatcatttc taactcctat
     2461 tatttagttg attgttcaaa ataataacgg cctgttttta atgcactagg gccaagacga
     2521 ccatatttga acattaaata catttctaca actaagaata gtgtatataa accacacaat
     2581 aagccgattg taaaccataa atcacctgtt gttaatgcag agtttgacac accgacaggt
     2641 aacatttcat agatagccca aggctgacgg ccatattcgg caaggaacca accagattca
     2701 atcgcaatcc aaggtaacgg aagaccgaat aacaagcctc ttaataagaa acggctactt
     2761 tctactttac cacgcatatt ttgaataaat gctgtcgtaa tcaagataag catcaagaag
     2821 ccagataaca tcattacacg gaatgcccag aatgtagggc ctacatttgg aattgtatta
     2881 tctgctgctt tttgaatttg ttcttcagtc gcatcaacca cattaggtgt atattgtttt
     2941 aacaataacc caaagcctaa atctttttgt acagctttaa atgcttcttt atccgcctct
     3001 gtataattgc cagaacgtaa tttttctaat agtccgtagg ctaccatacc ggtacgaaca
     3061 cgttgttcat taattacacg aatatctttt aggcctttaa tttctttatc taaagaacgg
     3121 gttgcaataa taccagctgc ataaggaata tgaattgaga attcattttt catttccgca
     3181 gtatttggaa tcacaaaagc attccaagca gctggagcag gctgagtttc gaattcacct
     3241 tccattgcag ctaatttagt cggctgagct ttaccgattt cataaccaga ttcatcaccc
     3301 ataatcagta cagagatgat cgcaattaaa ccaaaacttg cacctactga gaatgaacgt
     3361 tttgcaaagc ctaagtcacg accttttaag atatagtatg aactaatacc taatacaaaa
     3421 atagcaccac aaacatagcc tgcggttaca gtatgtaaga atttagattg tgtaactggg
     3481 tttaatacaa gttcagagaa acttgaaagc tccattcgca tggtttcaaa gttgaattca
     3541 gaagcaatcg ggttttgcat ccaaccatta gcaactaaaa tccatagtgc agagaagtta
     3601 gaaccaaacg caacacagta agttgctaat aaatgtttcg ctttcgataa acgttcccag
     3661 ccaaagaaga acaagcccac aaaggtagat tctaagaaga acgccattaa accttcaatc
     3721 gctaatggag cgccaaaaat atcacctaca taatgagaat aatatgacca gttcatacca
     3781 aactggaatt ccattgtaat accagttgtt acccctaggg caaagttaat accaaataat
     3841 ttaccccaga atttagtcat atctttatat acttctttac ccgtagttac atacagggtt
     3901 tccatcacca caaggacgaa agaaagacct aatgtaagcg gtacgaataa gaagtgatag
     3961 agtgcagtta gagcaaactg caaacgtgag agttcaacta catctaacat ctcaaacctc
     4021 ttatattaat atactaattt gacaattaga ttactgtaaa agtaatcacc cacaaattca
     4081 ctcaattcta ataaataacc caaattggtt aattttataa taaagtgtcg acgagtattc
     4141 tacctgtaaa tagctaatta aaaaatagta attttgttat at
//
LOCUS       DANKBV010000165         4177 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.165, whole genome shotgun sequence.
ACCESSION   DANKBV010000165 DANKBV010000000
VERSION     DANKBV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.165"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..576
                     /locus_tag="PWY43_002239"
     CDS             <1..576
                     /locus_tag="PWY43_002239"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248581.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer of AccA2D2 subunits; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HDL2137084.1"
                     /translation="KDSFIVLAGTLHNMPVVTASFNFEFMGGSMGSVVGAKFVKAAEK
                     ALAEKIPFICFSASGGARMQEALFSLMQMAKTSAILAKMKEQGVPFISVLTDPTLGGV
                     SASLAMLGDVNIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLEHGAVDMIVHRK
                     EMRDILARLCAKLTNQPTPFKVAELVVENAE"
     gene            592..1893
                     /gene="folC"
                     /locus_tag="PWY43_002240"
     CDS             592..1893
                     /gene="folC"
                     /locus_tag="PWY43_002240"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2137085.1"
                     /translation="MNTLSIPKATDSLETWLSYLEHAHSKPIDMGLDRIRKVATELDL
                     LKPAPYVITVAGTNGKGSTCRLLEVTLLKAGFRVGVYSSPHLIHYNERVRINGEVLPD
                     ADHVQAFAKINAKKTASLTYFEFSTLSAFELFKQNQLDIVILEVGLGGRLDATNIIDP
                     NLAVITSIDIDHVAFLGDNREDIGREKAGIFRENIPVVIGEPDCPHSIKQIADSLNCH
                     AFYRGKDWQFEIKNNLFHWYSEEKSFTDLPIPLIPIPNAGTALAVLMQLPFEIDEKII
                     RQALNEAQMTGRFQTLTDTDFTHFSQPKPAAKVIIDVGHNPHAARYLADKLRELKQPN
                     QKIYAVFSALEDKDLSGIVEPLANIIDEWHCAGLDCWRGQTGEAVLAKLVNVLPNSTA
                     CSYENVAQASRVLFETANENDIVLVFGSFHTVADFMLWLET"
     gene            complement(2000..3190)
                     /locus_tag="PWY43_002241"
     CDS             complement(2000..3190)
                     /locus_tag="PWY43_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HDL2137086.1"
                     /translation="MFSHIQAAPADPILGLGEAFKADSREGKINLGIGVYMTDEGKTP
                     IVKAVKEAEKRLLETENSKNYLTIDGVQAFNAATQALLFGEGAEVITSGRAKTAQSLG
                     GTGALRIAAEFIKRHTNAKNVWISTPTWPNHNGIFDAVGVNVKGYRYYNKETNGLDWE
                     NLIADLSQAEAGDVVLLHGCCHNPTGIDPTPAQWEQLAALSAEKGWLPLFDFAYQGFA
                     NGLEEDAYGLRAFVKNNRELLVASSFSKNFGLYNERVGAFTLIADNADDANRAFTQIK
                     SIIRVLYSNPSAHGASALAVALADPDLKAQWIAELDEMRNRIKEMRSQLVQKLKEKGA
                     NKDFSFITEQNGMFSFSGLTPEQVDKLKDEFAIYAVRSGRINVAGITSKNIDALADAI
                     VKVL"
     gene            complement(3298..>4177)
                     /locus_tag="PWY43_002242"
     CDS             complement(3298..>4177)
                     /locus_tag="PWY43_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HDL2137087.1"
                     /translation="LQEKIAFYHLLANAHYKTTPTDLRRLFDGENQLLFSQQENEQLI
                     GGIWAMQEGGLDEKLTQAIWLGERRPQGSLVAQYLCFQGNLPQACKLHSIRISRIAVL
                     PEKQNQGVGKRLISQFILHNSVEKRPLDFISVSFGLTTPLHAFWQSCGFQLVQITPNK
                     EASSGYQSAMMIYPISEQGKLFAQQATQAFARDFALQPFFTEICEELQNFEQFRPLAT
                     HNLDERDWKNLTAFAHGKRTFSAAYVSLKRLYLSDKARFSELDIFERKGQKISKMEVE
                     NYRKLVLQNLNNKSLK"
BASE COUNT         1179 a          878 c          877 g         1243 t
ORIGIN      
        1 aaagattctt ttatcgtgct tgccggtaca ttgcataata tgccggtggt tacggcctcc
       61 tttaactttg agtttatggg cggctcaatg ggttctgtgg tgggagcaaa atttgtgaaa
      121 gccgcggaaa aagctttagc tgaaaaaatt ccgtttatct gtttttcggc atcgggcggg
      181 gctcgtatgc aagaggcgtt attctcttta atgcaaatgg caaaaaccag tgcgatttta
      241 gccaaaatga aagagcaggg cgtaccgttt atttctgtat taaccgaccc aactttaggt
      301 ggggtttccg caagtttggc aatgctgggc gatgtgaata ttgctgagcc aaaagcccta
      361 atcggctttg ctggtcctcg tgtaattgag caaaccgtac gtgaaaaact gccggaaggt
      421 ttccaacgtg ccgagttctt attagaacac ggggcagttg atatgatcgt gcatcgtaaa
      481 gagatgcgtg atatccttgc ccgtctttgt gcaaaattaa ccaatcagcc aacgccgttt
      541 aaagtggcgg aattggttgt agaaaacgca gaataattta acttaatctg aatgaataca
      601 ttaagcatac ctaaagccac ggattctttg gaaacgtggc tttcctattt agaacacgcc
      661 cattctaaac cgattgatat gggcttagat cgtattcgta aggtcgcaac agagctggat
      721 ttactcaagc ctgcccctta tgtgattacc gttgccggta ctaacggcaa gggttcgacc
      781 tgccgtttgt tagaagttac cttattaaaa gccggatttc gggttggggt ttactcttca
      841 ccgcacttaa ttcactataa tgaacgggtg cgaattaacg gcgaagtatt acctgatgcc
      901 gaccacgtcc aagcatttgc aaaaattaat gcaaaaaaga ccgcttcact cacttatttt
      961 gagtttagta cgctgtctgc atttgagcta ttcaaacaaa atcagctcga tattgtgatt
     1021 ttagaagtcg gattaggtgg gcgattagat gccactaata ttattgatcc aaaccttgcc
     1081 gtgattacct cgattgatat cgaccacgtt gcatttttag gcgataaccg tgaagacatc
     1141 gggcgtgaga aagccggcat tttccgtgaa aatattccgg ttgtcatcgg cgagcctgat
     1201 tgccctcatt cgattaaaca gattgctgat tcgttaaatt gtcacgcttt ttaccggggt
     1261 aaagactggc agtttgaaat taagaataat ctgtttcatt ggtattctga agaaaaatcc
     1321 tttaccgatt taccaatccc gcttattccg attccaaacg ccggcaccgc attagcagta
     1381 ttaatgcaac tgccatttga aattgatgaa aagattattc gacaagcctt aaatgaagct
     1441 caaatgacag ggcgttttca aaccctgacg gataccgatt ttactcattt ttctcaacct
     1501 aaaccggctg caaaagtaat tattgatgta gggcataacc cacacgcagc acgttattta
     1561 gctgacaaac tgcgggagtt aaagcaacca aatcaaaaaa tctatgcggt gtttagtgcg
     1621 ttagaagata aggatttatc cggtattgtc gagccattag ctaatattat tgatgaatgg
     1681 cattgtgcag ggctagattg ttggcgtgga caaacaggcg aggcggtgtt agcgaaattg
     1741 gtaaacgttt tgccaaattc aaccgcttgt agctatgaaa atgtggcaca ggcaagccga
     1801 gtattgtttg aaacagccaa cgaaaatgat attgtgctgg tgttcggctc atttcatacg
     1861 gtagcagatt ttatgctttg gttggaaacc tagttctacg aataatcaac gttggtgcaa
     1921 gtgtccacac ttgtacctaa aagataaaaa agaggcacaa gcactcaggc ttatgcctct
     1981 atatatcttt aaggtttaat taaagcactt tcacaatcgc atcggcaaga gcatcaatgt
     2041 ttttgctggt aatgcctgct acattgattc tgcctgaacg cacagcataa attgcaaact
     2101 catcttttaa cttatccact tgctccggtg ttaagccact gaatgagaac ataccatttt
     2161 gctcagtaat gaaagagaag tctttgtttg cccctttttc ttttaatttc tgcactaatt
     2221 ggctacgcat ttctttgata cggttacgca tttcatctaa ttccgcaatc cattgtgctt
     2281 taaggtcagg atcagccaat gcaactgcta gtgcacttgc accgtgagca gatgggttag
     2341 agtaaagtac acgaataatc gatttgattt gagtgaaagc acggttggca tcatcagcat
     2401 tatctgcaat tagggtaaat gcaccgacac gctcgttata taacccaaag tttttcgaga
     2461 atgagcttgc gaccaacaat tcacggttat ttttcacaaa agctcgtaaa ccgtaagcat
     2521 cttcctctaa accgtttgca aagccttgat aagcaaagtc aaataacggc aaccagcctt
     2581 tttcggcaga taatgctgct aactgttccc attgtgcagg tgttgggtca attccggtcg
     2641 ggttgtggca gcaaccgtgc aataacacca catctccggc ttccgcttgg cttaaatcgg
     2701 caattaaatt ttcccaatct aaaccgttag tctctttatt gtagtagcga tagcctttta
     2761 cattcacgcc tactgcatca aaaatgccgt tatggtttgg ccaagtcggg gttgaaatcc
     2821 atacattttt cgcattcgta tgacgtttga taaattccgc cgcaatgcgt aatgcccctg
     2881 tcccacctaa actctgtgca gttttagcac gacctgaggt aatcacttca gccccttcgc
     2941 caaataataa cgcttgcgtt gccgcattga atgcttgaac accgtcaatg gttaaataat
     3001 ttttactgtt ttccgtctct aataaacgtt tttctgcttc ttttaccgct ttgacaatcg
     3061 gggttttgcc ttcatcggtc atataaacac cgataccaag gttgatttta ccttcacggc
     3121 tatctgcttt aaacgcttca cctaagccta aaattgggtc tgccggagca gcttgaatgt
     3181 gactaaacat tgacattgga gttcctcatc aaaatgaaaa ataaacagtg aatttatacg
     3241 attaaattgc agatttagca agagcaaaaa cacaaaaaat gtaataaaac ctataaatta
     3301 ttttaatgat ttattgttta agttttgcaa aacgagtttt cgataatttt ccacctccat
     3361 tttggagatt ttttgccctt ttcgttcaaa aatatccagc tctgaaaagc gtgctttatc
     3421 cgataaataa agccgtttta aactcacata cgccgctgaa aacgtccgct tgccatgagc
     3481 aaacgcggtt aaatttttcc aatcacgttc atctaaattg tgtgtggcaa gcggtcgaaa
     3541 ttgttcgaaa ttttgcaatt cctcacaaat ttccgtgaaa aatggctgaa gggcgaaatc
     3601 tctggcaaag gcttgggttg cctgctgtgc aaataacttt ccttgctccg aaatcgggta
     3661 aatcatcatt gcactttgat aaccactgct tgcctcttta ttcggtgtga tttgcacaag
     3721 ttggaaacca caagattgcc aaaatgcgtg gagtggagta gttagcccaa agctcacaga
     3781 gataaaatca agcggtcgtt tttctaccga attgtgcaaa ataaactgag aaatcaaccg
     3841 cttgccaacg ccttgatttt gcttttcagg caataccgca atccgagaaa tgcgaatgga
     3901 atgcaatttg caagcctgcg gtaaattccc ttgaaaacag agatattgag ccactagact
     3961 gccttgcgga cggcgttccc ctagccaaat ggcttgggtt aatttttcat ctaaaccacc
     4021 ctcttgcatt gcccaaattc cgccgataag ttgctcgttt tcctgttgtg aaaacaaaag
     4081 ctgattttcg ccatcaaata aacggcgtaa gtcagttgga gtggttttgt aatgggcgtt
     4141 ggcgagtaag tggtagaaag cgattttttc ctgtaaa
//
LOCUS       DANKBV010000166         4030 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.166, whole genome shotgun sequence.
ACCESSION   DANKBV010000166 DANKBV010000000
VERSION     DANKBV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.166"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            84..338
                     /locus_tag="PWY43_002243"
                     /pseudo
     CDS             84..338
                     /locus_tag="PWY43_002243"
                     /inference="COORDINATES: protein motif:HMM:NF017476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1470..1742
                     /locus_tag="PWY43_002244"
                     /pseudo
     CDS             1470..1742
                     /locus_tag="PWY43_002244"
                     /inference="COORDINATES: protein motif:HMM:NF017476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2814..3386
                     /locus_tag="PWY43_002245"
                     /pseudo
     CDS             2814..3386
                     /locus_tag="PWY43_002245"
                     /inference="COORDINATES: protein motif:HMM:NF017476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3387..4030
                     /locus_tag="PWY43_002246"
                     /pseudo
     CDS             3387..4030
                     /locus_tag="PWY43_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252915.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
BASE COUNT         1453 a          611 c          889 g         1077 t
ORIGIN      
        1 aggaaagcgt aactgtaggt gacaacttag cagttcaaca agataaaaat aataatacgg
       61 gtggtaagaa ctatcatgta acgttgaata acactttaaa cctgggtaat gatggttctg
      121 taacaattgg tgatacagtt attaatcaaa atggtttaac tattaacaat ggtggaccga
      181 gtgttacaac aataggtatt aatgctggta acaaagtaat tactaacgta gcagatggcc
      241 agaatgacac agatgcagta aatctccgcc agttaaaagc tgtgcaaact aaagtagaaa
      301 aaggtacaaa tgttgcatct gtcactgcaa ataaagatgc aacaggtgta acaacttata
      361 ctattaatgc tgatggtaca actgcgagtg cagatgaagc ttctaaagat ttcttaactg
      421 ttactacggg tgagaaaaac caagataatg taacagatta caaagttggc ttaactaaga
      481 aagcaacgga tgctttagcg aaggctgaaa ctgcgattca agagtttact acttcagtaa
      541 atggtcaaac tgttgaaaca attaacaaag acaacaaaga tgttggtttt gtaaatggta
      601 agggcacaat tgctcgtaat gataatagta acattacctt tgatgtagat gttgatgata
      661 atactattaa agtaggtgag gatggtaaat taactgttaa caaagaggca gttgatacta
      721 atacttatgt tactggtggc acagtagtgt atgctgataa tggcgatgca acaacaacgt
      781 taaacaaaaa tgatggttca tctgttactg taacaggcat taaaaataac tttgttaata
      841 aagcagaaac ctctacagat ggtaaaactg caactttaac tcgtaatgat ggtggtacag
      901 ttaatattga cctaactaat acggtaaaca cagctgtaaa tgaagcaaca gagaaaggct
      961 ttaagatttc agctgataaa ggtgatgaag atcacgttaa gctaggcgaa acagttaaat
     1021 atacctctac tgataaaaac attattacga ccgtaacgaa taaccaaatt gattttgcgt
     1081 taaattcaac tttagctatt ggtggcaaag atggtaaatc aggtattggt ttagatggta
     1141 ctaacggtac aatcggttta actggcccag cgggtaaaga cggtaaatct gcaacagcaa
     1201 atattactgt taaaaatggt caaccaggtg ttgatggtca agatggggag accaaaactc
     1261 gtattgttta tgatggtgaa gaagttgcaa cccttaacga tggcttaaaa tttggcgcta
     1321 acacaggtga tgttcacaat gcaaaattaa atacgcaagt gaatgtaaaa ggtgctgaag
     1381 acaataccga gtggaataag tttgatgcag gtcagaacat catgacccaa atcagcggta
     1441 ataatattac tgttgcattg gcgaagaatg tgaacttgac tgaagatggc tctgtaacta
     1501 ttggtggcac aaacattact aatgaaggta ttaccattaa taatggcccg aaaatcacta
     1561 ataaagaagg tagtgtaagt gttgctaatc aagatggaag tccaacaaaa attactaacg
     1621 tgaaagcggg tgatgtaaac aatacttcta cagatgctgt taatggaagc cagttatata
     1681 ctgtacaaga aattgcaaat gcaggttgga atttaactat taatgaagga cagaatagca
     1741 gtaatgttaa gcctaaagag acggttgatc taaataataa agatggcaac attcaaatta
     1801 ctaaagatgc ccataatgta acatttgcat taaataatga gttaactatt ggtggaccag
     1861 gtaaaaatgg tgctgatggt aaagacggct ctatcggtgt taaaggtgct gatggttcaa
     1921 ctggtgttgc tttaaatggt aaagatggtt caattggcat taacggtaaa gatggtgcta
     1981 atgcaaatat tactgttaaa aatggtaaac ctggtgttga tggcgtagat ggtacaacta
     2041 aaacacgtat tgtttatgaa accaaagatg gtccggaaga agttgcaaca cttaacgatg
     2101 gtttaaaatt cgttggtgat gatgatcaag ttattactaa gaaacttaac caaactttaa
     2161 atgtaactgg tggtgctgat ttaaccaaat tggctgataa caatattggt gtagtaaata
     2221 ctaataatgg attagttgtt aaattatcta aagatgtaaa cttaactaaa gatggttcgg
     2281 taactattgg taatacaact ttaaatgatt caggattaac tattgctaat ggtccaagtg
     2341 tgactaacac aggtattagc gctggtaaca aacagatcac caatgtatca agcggtttaa
     2401 ctaatgcaaa tggcgaaaaa actgatcttg ccaatgcaac gacaaccaat gcggttaacg
     2461 taggcgattt aaaagataca gttaataacc taactaatgc aacaacgggt ggctttggtt
     2521 taactggcga tgataagaat actgaagtta agcaagactt aggtaaaacg attcaagtta
     2581 aaggcaaaga tggtgtaacc gtaaccgcaa atgttgctga aaaagcatta gaaattgtat
     2641 tagccggtga tgtatctgta agtggtaaag acggaaaaga cggctctatc ggtgttaaag
     2701 gtacagatgg caaagacggt gtaactatca atggtaacgg cactgttgta gtcggcaaag
     2761 atggtgctcc aggtaaaatt ggcatcaacg gtaaagatgg tgcaaatgca acaatgactg
     2821 ttgagaaagg cgctcctgga ttagatggtg ctaaaggcaa ggatggtaaa gatggtactc
     2881 aaactactcg tatcgtttac acaaacgaaa caggtggtaa agaagaagtt gcaaccttaa
     2941 acgatggttt acgttttacc ggtaataacg aagttgagaa caaacataag ttaaacagct
     3001 tagttactat tattggtgag ggtgtaaata aagaacagtc aggcgctttc caatcagcag
     3061 caggcaatat taatgttgtt gcagatggca atggtaattt aactgttaaa ttagcgaaaa
     3121 acctaaactt aactgattca ggttctgtaa ccattggtgg cacaaacatt actaatgaag
     3181 gtattaccat taacaatggt ccgaaaatca caaatgatgg cgataatgtt aaagttggtg
     3241 atgtaaatgg tgcgccagtt aaaatcacga atgttaaaga aggtgattta agcgaaactt
     3301 ctaaagacgc agttaacggt tcacagcttt atgcaactaa ccaaaatgta accaacttaa
     3361 gcaataaagt agctcaaggc ttcaatatta ctgctgataa tggtacggct gacaatgttc
     3421 aattaggtga aactattacc tatacttcat ctgataaaaa tatcgttaca acggtaagcg
     3481 acaataagat cgattttaaa ctgagtgctg aagctgtaga taaatttacg gttaaatact
     3541 tctctgtaaa ttcaacggtt gcagaaaaca gattaaatga tggtgctaaa ggtgttaact
     3601 ctattgcaat tggtccgaat gcaacctctg tagatcaagg tgctattgca ctgggtacaa
     3661 actcaaatgc aaatggtaat gctgcagcgg ctttaggtgt caactcaaaa gctgaaggta
     3721 ttcaagcaac tcggtaggtt ctcaagcaaa tgcaactgca aatggtacta ctgcgattgg
     3781 tcgtgaaaca acagcaagtg caggtgaggc aacagctgtg ggttctaacg caaatgcaac
     3841 atcagagaaa gcaactgctt taggtgtagg ctcaaaagcg acagataaag cagcattagc
     3901 tgtaggtaca aatgcaactg caagcgcaca gtctgcagta gctgtaggta ctcaatcggt
     3961 agctgataaa cagttcgctg ttgcagtagg tatgggggct caagcaacct tagaaaactc
     4021 tgtagcatta
//
LOCUS       DANKBV010000167         3993 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.167, whole genome shotgun sequence.
ACCESSION   DANKBV010000167 DANKBV010000000
VERSION     DANKBV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.167"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            150..1955
                     /locus_tag="PWY43_002247"
     CDS             150..1955
                     /locus_tag="PWY43_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL2137088.1"
                     /translation="MLSKLQRFTLMIKSTIIKHPLEILFVTYVTILLINNVSDYELIK
                     RFQNLIALFPICFILLYLIRDTKAYWLFALIPIAIVFFFDQINLTLLEDSRYWATIFC
                     VFLCLISQYWYKNNHYFVAGTTNKLVNIVFATVLSGIIFATLSAITIAIIALFDLNKQ
                     NNFHIFEKFGIFSSFWAFPVLFLTLEQQTTNDSRYLNRISEMLLNWILSPALIIYTAI
                     IYAYVIFIAAKGQMPDGVVANVALPYLAFGLAIQAVQLLLKNAKWQWFYSLFTYLALL
                     PLTLIWYAIYIRFSHYGLTEARIYLVIGTITLSICYTLLLFKQLAQYRLFSAVSIAII
                     LISVFILDPKTVALKDQTKRLDNYLLKHKLLDVNNKISTKAVLKYQESLNENRDEAEY
                     FYSMLHYVARGMPAAQFKEKYGVNSSYDLMSEIRERNTDPRYFEATNSELVRLTKADM
                     ANAREYITFSSQLYRPNSFSHQKIENKLCQKLLSQGYYFEGNILNIEYQYSETAQDCS
                     SITQDPTEFVIYFKGLGTTIEFNINDILKKIFAKHHLSITQQHKNEVLEKIKGDLLKM
                     DLGNSVLGLKHIELRFEDNIGYIVESISTQYIMLK"
     gene            complement(1998..3993)
                     /locus_tag="PWY43_002248"
                     /pseudo
     CDS             complement(1998..3993)
                     /locus_tag="PWY43_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249500.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
BASE COUNT         1171 a          784 c          765 g         1273 t
ORIGIN      
        1 atttttaaag cctatagttg aataacttag gctttcttat tttaatgaat atgttcacct
       61 ctaaaaacaa ataaagatta aaactgttta cctattattt attcacatgt atactgtgag
      121 atattccttt cataggtagg agatctatta tgctaagtaa gctacaacgc tttaccttaa
      181 tgataaaaag caccatcatt aagcatccac tggaaatttt attcgttact tacgtcacga
      241 ttttacttat caataatgtg agcgactacg aattaatcaa acgctttcaa aatctaattg
      301 ctctttttcc tatttgcttt attttgcttt acctgatacg cgataccaaa gcttattggc
      361 tattcgctct gattccgata gcaatcgtct ttttctttga tcaaattaat ttaacattat
      421 tggaagattc tcgttattgg gctaccatat tttgtgtatt cctttgtttg atcagtcaat
      481 attggtacaa aaacaaccat tattttgtag ccggaacaac taataaatta gttaacattg
      541 tctttgctac agttttatct ggcattattt ttgcaacatt aagcgctatt accatagcca
      601 ttattgcttt atttgattta aataaacaaa acaactttca tatttttgaa aaatttggca
      661 ttttttctag tttttgggca ttccctgttt tatttttaac tcttgaacaa cagactacca
      721 atgattctag atatctaaac cgcattagtg aaatgttact aaactggatt ttgtctccgg
      781 cattaattat ctatactgca attatttatg cttatgtaat ttttattgcg gctaaggggc
      841 aaatgccaga tggggttgtt gcaaacgtag cgcttcccta tttagctttc ggcctagcta
      901 tacaagcggt gcaattatta ctaaaaaatg caaaatggca gtggttttat tctctcttta
      961 cctatttagc acttttgccg ttaacattaa tttggtatgc catctatatt cgtttcagcc
     1021 attatggctt aactgaagcc cgtatttatt tagtgattgg cacaataacc ttatctattt
     1081 gctatacctt attactattt aaacagttgg cgcaatatcg tctcttttct gcagtgtcga
     1141 tagcaattat tttaatttca gtgtttattt tagatccgaa aacagttgcg cttaaagatc
     1201 aaaccaagcg gttggacaac tatttgctaa aacacaaatt attagatgta aataacaaaa
     1261 tcagcacaaa agctgtactt aaataccaag aatcattaaa tgaaaaccga gatgaagccg
     1321 aatattttta ttcaatgctc cactatgttg ccagaggaat gcctgctgca caatttaaag
     1381 agaaatatgg cgtaaattca agctacgatt taatgagtga aataagagaa agaaatactg
     1441 acccacgtta ttttgaagct actaattctg agctggttcg cttaactaaa gcagatatgg
     1501 caaatgctcg tgaatatatt acatttagta gccaattata tcggcctaat tcattttccc
     1561 atcaaaaaat agaaaataag ctatgccaaa aactcctttc tcaaggttac tattttgagg
     1621 gaaatatatt aaatattgag tatcaatact cagaaacggc tcaagactgc tcttcaataa
     1681 cacaagatcc tactgaattt gttatctact ttaaaggact tgggactact attgaattta
     1741 acattaacga tattttgaaa aaaatctttg ctaaacatca tttatctatt acccagcaac
     1801 acaaaaatga ggttttagaa aagattaaag gcgatttact caaaatggat ttaggaaatt
     1861 cagtacttgg gcttaagcat attgagttaa gatttgaaga caatatcggt tatatagtag
     1921 aaagtattag tacacaatat attatgctga aataaaagga gatgctcgtt caattaaatt
     1981 tgaacgagca tttttcgtta aaaattcaat ttaagcagcc actcaaaatt agtctcattt
     2041 aaccgatatt gcggtttcaa attcgggccg caagagtttg agccaatgcc gctcattttg
     2101 taatcgacac acagcacggt ggaagggctt tctaccaaat cataactgtg gcttttagtg
     2161 gttagctctt cctgtgtgta aggcgaaaga ttaaaactaa acggctgtgg agaagtaaca
     2221 gttatcccat ttgcttgcac ataatgcgta ccgaagtggc tgccgttttc ctgcgggttg
     2281 agataatcgg tatgattttc tcgggtgctg gtttgataag ctccgagttt cgccaaatgg
     2341 tatttgtcga tataactctc ctgctcgcca tagccaaaat agctcacttg gttattccgt
     2401 ttattcaaga aaaaccgcaa gccaaagcga ggtaaaaacg gcaaatgagc ggggcgggtt
     2461 gcctgaacct gaatcgtcat ttcgccattc ggggcaagat gataattcac tttcagggtt
     2521 aaaatccgac tttgagcggt tgccacaatg ccacattcgg cagtaatgtt tacgccgttt
     2581 ttatcttgca tcagttcggt atgatacgcc ctggtgtaac agttatcgta gccggctttt
     2641 ttccaaaact cacgaattaa gcggtcgtta tcggttgggg ctcgccaaat attgaaatcc
     2701 aacggttgct caattactgc ctcgccgttt ttctcaatcc ggctgaaaat gcctttgagt
     2761 ttatcgaaag tgtagcgata gtcgccctgt tgaatccgcc attcatttgc aaaatcttgc
     2821 acaaaaatga ccgcttgcgg gcgtggatta agtgctggag tgaccatcgc ttgatgcagc
     2881 gtgatttggt caaagcctaa gtgatgccct ttttccaaca gagccgtgct ttcatttaaa
     2941 gaataatcaa gggttaaaag ttgcaatttg ccgtttggtt cgggcagcgt tagcggcaaa
     3001 caagcggtct gatttggctg acaatttacc aaacattgtc cctgcagggt ggtttcgccg
     3061 ttttcgctga aacagtaatg cacggtcaga taatcagcta agtcgctgaa atccaactga
     3121 tttttcagcc aaattttgcc cgcttttaac tcagctcgca ccgggcgatt cacatttttc
     3181 acttctaaca aattagagtg tggcacacgc tgttgggaaa ccaagccgtc catacagaaa
     3241 ttgccatcgt gcaaggtttc gccgaaatca ccgccatagc cgtatttttc ggtcttctgt
     3301 tcggcactta gctcagagtt ggggagcaga ggagcatggt cgcaccattc ccacacaaag
     3361 ccaccgcacg caccgctgta gcgttggaag gtttgccaat aatcttccat atcaccactc
     3421 gaattgccca tcgcgtgaga atattcacac aaaataaaag gtttgtggtt ggcagagtcg
     3481 gaaaaatatt cctctaaccg ccggtgcttg ggtacatctc gctgtataaa tcgaggtttg
     3541 aaagatcgtt ttgatgtgcc gaatgttggt aaatcgagcc ttcgtagtga gttaaacggc
     3601 ttggatcccg ttgttttacc caacgggctg cgacttcaaa attttcacca aagccggatt
     3661 cgttaccaag tgaccaaata ataaccgaag tgcggttttt gtctcgctcc acattggcaa
     3721 tgttgcgatc taaaatcgcc tgtttgaaca gcggatctct tgcaaaataa cagaaattat
     3781 cgaccgcttg ttggcgaatt tcttcctgtt tgttcgggtt tggcacattc aggaaaatgc
     3841 tgttttccgg actttcaata tacactgcac tggagccgtg agattcaaga tcactttcgg
     3901 caatcagata aaaaccgagc tgatcgcaca attcattaaa ccacggagcg ttcgggtaat
     3961 gggcggtgcg aatagcgttg atgttatggg ctt
//
LOCUS       DANKBV010000168         3950 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.168, whole genome shotgun sequence.
ACCESSION   DANKBV010000168 DANKBV010000000
VERSION     DANKBV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.168"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(112..1050)
                     /gene="yvcK"
                     /locus_tag="PWY43_002249"
     CDS             complement(112..1050)
                     /gene="yvcK"
                     /locus_tag="PWY43_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDL2137089.1"
                     /translation="MENTDIIPFKPTRHHYLNQLNAVVALGGGHGLGRLLSALSFLKE
                     RLTGIVATTDNGGSTGRIRSQQGVIAWGDLRNCLNQIIVKPTIASRLFEYRFAGTGEL
                     AGHNLGNLILTALENMQIRPLEGINLVRELLHVRSQLIPMSEIPVHLIAQLHSGESVF
                     GEVQIDSLAETPQHLALDTMVVATPEAISAIQSAELILLGPGSFFTSIMPNLLVAGIA
                     NSIAQSKARVVFIDNIGQEHGPAGQLSLNQRIEWMENCIGQARINAVITTDDAGSELP
                     EHIQILRKNLSANDVYYRHDRNKLSKAIDELLAQNI"
     gene            complement(1064..2140)
                     /locus_tag="PWY43_002250"
     CDS             complement(1064..2140)
                     /locus_tag="PWY43_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2137090.1"
                     /translation="MFQMLQDWYRKKFTDPQIVVLFSILLIGFGIIYFFSDLLMPLLV
                     ALVFAYLLEWPIRFLSSKLKLPRTLSVILVLGGFIALLSFLGVVLLPSLWNQAVTFIQ
                     DLPSMFNLLNAWLQALPEHYPELVDYATLDSIVNTAKSNILSMGESLLTLSINSIISL
                     VGLGIYTFLVPLMVFFLLKDKIVLMRSFSKVLPQNRRLATRVWFEMQQQIANYIRGKF
                     LEILIVGVVTYIIFLFFDLRYPLLLSVAVGISVLVPYIGAVLVSIPVMLIALFQFGLS
                     PDFYYLMLAFIISQLLDGNLLVPFLFSEAVNLHPLTIIIAVLIFGGLWGFWGVFFAIP
                     LATLVKAVLQALPSAQSDAIILEK"
     gene            complement(2171..2926)
                     /locus_tag="PWY43_002251"
     CDS             complement(2171..2926)
                     /locus_tag="PWY43_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2137091.1"
                     /translation="MKKEEVGHHFLARLGKTRLRPGGKLATDWLIANGDFDKNKKVLE
                     VACNMGTTAIQLAQQFECQIVGIDLDEDALEKARKNIKENHVEHLIQVQRANATKLPF
                     DDESFDIVINEAMLTMLPLEAKEKAIREYFRVLKPNGFLLTHDIVIQDKDSDTLLAEL
                     RDAINITVSPLAKADWKSLFQRCGFRNVDTFSGEMSLLSPKGLIHDEGVLGAMKIIGN
                     ALKPENRETFNKMYKLFNDPDKKLGFIAVCSQK"
     gene            3078..3287
                     /locus_tag="PWY43_002252"
     CDS             3078..3287
                     /locus_tag="PWY43_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HDL2137092.1"
                     /translation="MSKQKNDYLHQRGQIKESAVKALVTDPLFRQRVERNRKGKGSYR
                     RQEKHKKTIYGQKETPFKNIRLESF"
     gene            complement(3336..>3950)
                     /gene="bioD"
                     /locus_tag="PWY43_002253"
     CDS             complement(3336..>3950)
                     /gene="bioD"
                     /locus_tag="PWY43_002253"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDL2137093.1"
                     /translation="KPIACGGDDSLPTEPTQYDYATEDNSDVLTILDSCPTNVSYRDI
                     NSYTFIHSSTPVFAALDAVHHIQLEKLNTDLKRLENAYPNVVVEGTYGWLTPINKDYY
                     FADWVMQNNMPVVLVVGIKEGCVNHALLTANEILQRGVKLVGWVANRVNPGLRHYHEL
                     IELLTQKINAPLLGQISYMGHPEHKELSSYIQNPEPLLAYFSKP"
BASE COUNT         1280 a          739 c          757 g         1174 t
ORIGIN      
        1 gcaaagccac caaatggatt gttgattatc tagacggtaa atgggttgag cgtaagaaat
       61 aaagcttaaa agacaagcgg taaaattttc taaaaatttt accgcttgct tttagatgtt
      121 ctgagcgagt agttcatcaa ttgctttact taatttattg cgatcgtgtc ggtaataaac
      181 gtcattcgca gataagtttt ttcgcaagat ttgaatatgt tcgggaagtt cagaaccggc
      241 atcatcagtc gtaattaccg cattaattct agcctgtccg atacagttct ccatccattc
      301 aattcgctga ttaagtgata gttgcccagc tgggccatgt tcttgtccga tattgtcaat
      361 aaatactact cgggctttgc tttgagcaat agagtttgct atgcctgcca ccagcaaatt
      421 cggcataata ctggtaaaaa agctacctgg gcctagcagt attaactcag cactttgaat
      481 agctgaaatg gcttctggtg tagcaacaac catagtatct aacgctaaat gttgtggtgt
      541 ttctgctaaa gaatcaattt gcacttcacc aaatactgat tcgcctgaat gcaactgtgc
      601 aattaaatgc accggtattt cagacattgg gataagttgc gatcttacgt gcaataattc
      661 tcgcaccaaa ttaatccctt ctagcggtcg gatttgcata ttttcaaggg cggtgagaat
      721 taaattgcct agattatgtc cagcgagttc gcctgtgcca gcaaaacgat actcaaacaa
      781 ccgagaagct atcgttggtt taacgataat ttgatttaaa cagttgcgta aatctcccca
      841 agcaatcacc ccttgttgtg aacgtattct accggttgag cctccattat cggtagtggc
      901 aacaatgccg gtaagccgtt cctttaaaaa agagagcgcg gagagcaatc gacctaagcc
      961 gtggccccca cctagtgcaa caactgcatt gagttggttt aaatagtgat gtcgggtagg
     1021 tttaaaagga ataatatctg tattttccat cttgggattt cgtttatttt tctaaaataa
     1081 tggcatctga ctgggctgaa ggcaatgctt gtaatacagc tttaaccaaa gtcgctaaag
     1141 gaatcgcaaa aaacactccc caaaagcccc ataaaccacc aaagatgagt acggcaataa
     1201 taattgtcag agggtgtaga tttactgctt cagaaaataa aaagggtacg agtaaatttc
     1261 cgtctagcaa ctgactaata ataaatgcga gcattaaata gtagaaatcc ggcgataacc
     1321 cgaactggaa tagagcaatt aacattaccg gaatgctgac taaaaccgca ccgatgtagg
     1381 gaactaacac tgaaatccct accgctacag aaagtagtaa cggatagcgt aaatcaaaaa
     1441 agaggaaaat aatataagtg acaaccccaa caattagaat ttctaaaaat ttacctcgaa
     1501 tatagttggc aatttgttgt tgcatttcaa accatactcg tgttgcaagt cgacggtttt
     1561 gcggtaatac tttactaaaa gaacgcatta aaacgatttt atctttcagt aagaaaaaga
     1621 ccatgagtgg gactaagaag gtgtaaatgc ctaagccaac taagctgatg atagagttaa
     1681 tcgagagtgt tagaagtgat tctcccatac tcaaaatatt acttttagcg gtattcacaa
     1741 tggaatctag ggtcgcataa tctactaatt cagggtaatg ctccggtagg gcttgtaacc
     1801 aagcattgag cagattaaac atcgaaggta aatcttgaat aaaggtaact gcttgattcc
     1861 aaaggctggg tagtagtact acgcctaaaa aagataataa agcgatgaaa cctcctagta
     1921 ctagaatgac actcaaagtt cgaggtaatt tgagtttgct cgacaaaaaa cggattggcc
     1981 attccaataa ataggcaaac acaagtgcaa ccagaagggg cataagcaga tcgctaaaaa
     2041 aatagataat accgaaaccg atcaatagaa tactaaacag cacaactatt tgcgggtcgg
     2101 taaatttttt tcgataccaa tcttgcaaca tttgaaacat atggattgcc tttaaattat
     2161 gcgatgaaaa ttatttctgg ctacagactg caatgaaccc tagctttttg tccgggtcat
     2221 taaatagttt gtacatttta ttaaaggttt cgcgattttc tggttttaat gcattgccga
     2281 taattttcat ggcgcctaaa acaccttcat catgaatcag acctttaggg gaaagtagag
     2341 acatttctcc ggaaaaggta tcaacattac ggaaaccaca acgttggaat aatgatttcc
     2401 agtctgcttt agcaagtgga gagacagtga tattgattgc atcacgcaac tcggctaata
     2461 aagtatcgga gtctttatct tggataacaa tatcgtgcgt taataaaaag ccatttggtt
     2521 ttaatacacg aaaatattca cgtattgcct tttctttcgc ttctagagga agcattgtca
     2581 gcatggcttc attaattaca atatcaaaac tttcgtcgtc aaatggcagt tttgttgcgt
     2641 tggcgcgctg tacttgaatt aaatgctcaa catggttttc tttgatattt ttacgtgctt
     2701 tttcaagcgc atcttcgtct aaatcaatac caacaatttg gcattcaaat tgttgagcta
     2761 attggattgc agtagtcccc atgttacagg caacttcgag cactttttta tttttatcaa
     2821 aatccccgtt tgcaattaac caatcagttg ctaatttccc cccggggcgt aaacgagttt
     2881 tgcctaaacg agctaaaaaa tgatgaccaa cttcttcttt tttcataaaa acctctggat
     2941 ttgcaaaata cgttaaatat atgaccgctt gtattgtgat ctatgttcgt tattatactc
     3001 aattttttca ttttcttata tacaacatca acagattttg ccagaataca acctcatttt
     3061 gatatgagga aaatagaatg agtaagcaaa agaatgatta cctccaccag cgaggtcaaa
     3121 ttaaagaaag tgcagtaaaa gcattagtga ctgatccact ttttagacaa cgtgtagaaa
     3181 gaaatcgtaa agggaaaggg agctatcgca gacaagaaaa acacaaaaaa actatctatg
     3241 ggcaaaaaga aaccccattt aaaaatattc ggttagagtc tttttaaatg gggttttatt
     3301 tgtagcttat tgtagtttta taagcctgat aaacactatg gtttactgaa ataggccaac
     3361 aacggttctg gattttgaat ataagaagaa agttctttat gctctgggtg acccatatag
     3421 gaaatttgcc caagtaaagg cgcattaatt ttttgtgtca gtagttcaat taattcgtga
     3481 taatgccgta gccctggatt tactctgttt gcgacccaac ctactaattt tacaccacgc
     3541 tgcaaaattt cattcgcagt aagtagtgca tggttaacac aaccttcctt aattcccacc
     3601 actaaaacaa ccggcatatt gttttgcatt acccaatcag caaaatagta atccttgtta
     3661 ataggagtta gccagccata ggttccttct acgacaacat ttggataggc attttctagt
     3721 cgttttaaat ctgtgtttaa tttttccagt tgaatatgat gaactgcatc taaagctgca
     3781 aatacagggg tactggaatg aataaaagta tagctattaa tatcacgata gctaacatta
     3841 gttggacagc tatctagtat ggttaatacg tctgaattat cttcagtagc ataatcatat
     3901 tgagtaggtt ctgtgggtaa tgaatcatca ccgccacaag caatgggttt
//
LOCUS       DANKBV010000169         3946 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.169, whole genome shotgun sequence.
ACCESSION   DANKBV010000169 DANKBV010000000
VERSION     DANKBV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.169"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            246..1610
                     /gene="glmU"
                     /locus_tag="PWY43_002254"
     CDS             246..1610
                     /gene="glmU"
                     /locus_tag="PWY43_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL2137094.1"
                     /translation="MTQLSVVILAAGKGTRMYSDLPKVLHPVAGKPMVKHVIDTAKQL
                     DAQQIHLIYGHGADLLKAHLSEEPVNWVFQAEQLGTGHAMQQAAPFFADDENIVMLYG
                     DAPLITKATLERLIAAKPENGIALLTVELENPTGYGRIIRENGSVVAIVEQKDANEAQ
                     LKVKEVNTGVMVASGASFKKWLKNLNNNNAQGEYYITDVIAMANQEGFKVQAVQATEF
                     MEVEGANNRLQLAALERFYQKREAEKLLLAGVAIIDPSRFDLRGTVTHGKDVQIDVNV
                     ILEGEIKLGNNVKIGAGCVLKNCEIGDNVEIKPYSVIEDSIVGAKSAIGPFSRLRPGA
                     ELAEETHIGNFVEIKKATIGKGSKVNHLTYVGDAEIGKECNIGAGVITCNYDGANKFK
                     TIIGDNVFVGSDSQLIAPVTIASGSTIGAGATVTKDIAENELVISRVPQRHIQGWQRP
                     TKKK"
     gene            complement(1763..2830)
                     /locus_tag="PWY43_002255"
     CDS             complement(1763..2830)
                     /locus_tag="PWY43_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL2137095.1"
                     /translation="MKKTLVALAVTAFAASASATTVYDAEGSKVDFDGSLRIVLDRTN
                     SKELNNHNVVKTKKDAHTGIRNTGSRFGVRVKHDLAQDFYALGRVEFRFNKNTSDDGF
                     GDLYTKRAYVGLGSKQYGELTFGRQLTIADDLSQSVDYNYGINPKGDYIQTDGNQVIR
                     YNYRGVENLHVSANYNFAQDRKDGEVLANKLQNAYGIGALYTVDNWDLRAAYGHTNYK
                     TATDSTKHRKDGVLGSVGYKFGDLKLSVDSGYRHEKVENEKGNAFYVSPNFEYQVVPA
                     SKVYGNYLYEREKVKKVSKEKTHGFLLGVDYQFHKQVVAFVEGSYTRTSTKDYNKVTN
                     GYKYTGKSTNKAIGVGMRVYW"
     gene            3333..3851
                     /locus_tag="PWY43_002256"
     CDS             3333..3851
                     /locus_tag="PWY43_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein"
                     /protein_id="HDL2137096.1"
                     /translation="MQKKLFIAQIKQNLSELNVFSTDNIFLNSPYFSQQTGLVSVFIA
                     EIEKTVELLLNQTEVLYSEFYAEKLVKQFDALKNAVEKIQSKPESAQFHSSYQFSPNI
                     HRLAPNKRLQEYRKALRALNEKISWLVEQNLNTQNEATKQTLQNQITETEYRKMKCLK
                     AIENLEQELLFK"
     gene            complement(3855..3928)
                     /locus_tag="PWY43_002257"
     tRNA            complement(3855..3928)
                     /locus_tag="PWY43_002257"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3894..3896),aa:Cys,seq:gca)
BASE COUNT         1350 a          734 c          686 g         1176 t
ORIGIN      
        1 aatttaatat tttagcaaag atatacacta ttgtagagag atagcacaaa taagtgctac
       61 ctatttatac cagttttcca caagataaga atcgacataa tagaaaaaga taacaaacag
      121 acaaatttct tttgcttgtc gatgaaatcc acttataaaa aggttaaaat attacaaaaa
      181 tatcacttat agcaatttac acgttactat aagccttcat ctatttagtc aaataaggat
      241 tctatatgac tcaactgagc gttgttattt tagctgccgg taaaggcact cgtatgtatt
      301 cagatttacc caaagtgtta cacccagttg ccggtaaacc catggtaaaa cacgttattg
      361 atactgccaa acagctagat gcccagcaaa ttcatttaat ttacggacac ggtgccgatc
      421 tgttaaaagc acatttaagc gaagagcctg taaactgggt attccaagcc gaacaattag
      481 gcacaggaca tgcaatgcaa caagcagctc cttttttcgc tgatgatgaa aacattgtaa
      541 tgctttatgg tgatgccccc ctaatcacaa aagccacttt agagcgttta atcgcagcca
      601 aaccggaaaa tggtattgca ttactaaccg ttgaattgga aaacccgaca ggctacggac
      661 gaattattcg tgaaaatggt tctgtggtag cgattgttga gcaaaaagat gccaatgaag
      721 cacagcttaa agtcaaagaa gtgaataccg gcgtgatggt agcaagcggt gcgagcttca
      781 aaaaatggtt gaaaaaccta aataacaaca atgctcaagg tgaatattac atcactgatg
      841 tgatcgcaat ggcaaaccaa gaaggcttta aagtacaagc ggttcaggca actgaattta
      901 tggaagtaga aggggcaaac aaccgtttgc aacttgctgc acttgaacgg ttctaccaaa
      961 aacgtgaggc ggaaaaactg ttattggctg gtgtagctat tatcgaccca agccgttttg
     1021 atcttcgtgg cactgtcacc cacggaaaag atgttcagat cgatgtgaat gtcattctag
     1081 aaggtgaaat caaactcggt aacaatgtaa aaattggtgc aggctgcgta ctgaaaaact
     1141 gcgaaattgg cgataatgtt gaaattaaac cttattctgt gattgaagat tcgattgtcg
     1201 gggcaaaatc cgctatcggt ccattctctc gcttacgccc gggggcagaa ttagcggaag
     1261 aaacccatat cggtaacttt gtggaaatca aaaaagctac catcggcaaa ggttcaaaag
     1321 taaatcatct cacttatgta ggcgatgcgg aaatcggcaa agaatgtaac atcggagcag
     1381 gtgtaatcac ttgtaactat gatggtgcga ataaattcaa aaccattatt ggcgataacg
     1441 tatttgtcgg ctcagacagc caactcattg ccccggtcac gattgccagc ggttcaacca
     1501 ttggtgccgg tgcaacagtc actaaagaca ttgcggaaaa tgaactggtt attagccgtg
     1561 tgccacaacg ccacattcaa ggctggcaac gcccgactaa aaagaaatag tgccagtggt
     1621 caaaagttat tatatttaaa aattggcttt gctaatactt gcaaagccat acgcttaaaa
     1681 aaacataatg aaccatatat ttaaaacaaa acccctaaca ataaatattg ttaggggttt
     1741 atcaatttag gaaatcaatt gattaccagt atacacgcat accaacaccg atagctttgt
     1801 tagttgattt gcctgtgtat ttataaccat tagttacttt attgtaatcc ttagtactag
     1861 tacgagtata agaaccctca acaaaagcaa ctacttgttt atggaattgg tagtcaacac
     1921 ctaataagaa gccatgagtt ttttctttac ttaccttttt cactttttca cgctcatata
     1981 ggtagttacc atatacttta gatgctggta ctacttggta ttcaaagttt ggagatacat
     2041 agaaagcatt tcctttctca ttttctactt tttcatgacg atagccagag tcaacactta
     2101 attttaagtc accaaattta taaccaacag aacctaatac accatcttta cggtgcttag
     2161 tgctatcagt agctgttttg tagtttgtat gaccataagc tgcacgtaaa tcccagttat
     2221 ctacagtgta taatgcacca atgccgtatg cattttgaag tttatttgct aatacttcac
     2281 catctttacg atcttgtgca aagttatagt ttgcacttac atgtaagttt tcaacaccac
     2341 ggtagttata acgaataact tggttaccat cggtttgaat ataatcgcct tttggattaa
     2401 taccgtagtt gtaatctact gattgactta aatcatcagc aatcgttaat tgacgaccaa
     2461 aagttaattc accatattgc ttactaccta aaccaacata agcacgttta gtatataaat
     2521 caccaaaacc atcatcactg gtatttttgt taaaacggaa ctcaacacga cctaatgcat
     2581 agaaatcttg agctaaatca tgttttactc ttacaccaaa acgtgaacca gtgttacgga
     2641 ttcctgtgtg tgcatctttt ttagttttaa caacattatg attgtttagc tctttagaat
     2701 ttgttctatc aagaacaata cgtaaagaac catcaaaatc tacttttgaa ccttctgcat
     2761 cgtaaacggt ggttgctgat gctgacgctg cgaacgcggt tactgctaat gcaactaatg
     2821 tttttttcat aattttcacc ttagatttta tatgttatat gataagtaat taccctcatt
     2881 aaggtgtaag agtaattatc caagatacaa tgtatcagca aaacgcctaa cattgttgtc
     2941 gccataataa ttgcaaaaag aaaactcaat gtcaatagtt caaactccaa aacaaagacg
     3001 gagtttttct tttaaaatca aatggttata attacattat ttcatttgga gaaaatccac
     3061 tcatttttaa cagaaaaaat gaagcgcata acatattgat aaataaggat ttttcatatt
     3121 taatttgata tagttcaaac ttatcaaaaa gttcatttat tttgtgacat agttcacatt
     3181 tttgatattt aatttctttt ttgcctgttt ttcacgcacg aattgtaaga aattagtaaa
     3241 aatcaaccgc ttgtacgcaa agtgttttgc tttaaaatag aagttatatg aaataaaatt
     3301 cacttaaatt ttctttattg ataacatcaa ccatgcaaaa aaaacttttt atcgcccaaa
     3361 taaaacaaaa cctctcggaa ttaaatgtat tttcaacgga taatattttt ctgaattcac
     3421 cctatttctc acagcaaacc ggtttggtga gtgtgttcat tgctgaaatt gaaaaaacag
     3481 ttgagctatt attgaaccaa actgaggtgc tatatagtga attttacgct gaaaaattgg
     3541 ttaaacaatt tgatgcatta aagaatgccg tagaaaaaat ccaaagtaag ccagaatcag
     3601 ctcaattcca ttcttcctat caattttctc caaatattca tcgtttagcg cccaacaaac
     3661 gcttacaaga ataccgtaaa gcgttacgtg cattaaatga gaaaattagc tggctagttg
     3721 aacaaaattt aaatacacaa aatgaggcaa ctaaacaaac cctgcaaaac caaatcacag
     3781 agacagaata tcgcaaaatg aaatgcttaa aggcgattga aaatttagaa caagagttgc
     3841 tatttaaata gtgatggagg cgtgtcccgg agtcgaaccg agctacatgg atttgcaatc
     3901 cagtgcataa ccgctttgct aacacgccat aagatttgaa gatttg
//
LOCUS       DANKBV010000170         3928 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.170, whole genome shotgun sequence.
ACCESSION   DANKBV010000170 DANKBV010000000
VERSION     DANKBV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.170"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1018)
                     /gene="glnE"
                     /locus_tag="PWY43_002258"
     CDS             complement(<1..1018)
                     /gene="glnE"
                     /locus_tag="PWY43_002258"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDL2137097.1"
                     /translation="MLTTLIPLSQLNLPPNLQNSEQIQPLVTAISLSNFVHQTLQKQP
                     DLLAEWLAKFPTPDDCCNYTERLAKILARVENEEMLGRVLRQFRHRELARLSFIQSNK
                     LATVELVFQHLSDLAESLILAARDWLFQRCCAEYGTPKNTLGETQELLILGMGKLGGR
                     ELNFSSDIDLIFTYPDIGETEGGRKPIENSKFFTRMGQRLIKVLDEITADGFVYRTDM
                     RLRPFGDSGALVLSFTAMEDYYQEQGRDWERYAMIKAKILGEDSQNLNHRYLKQMLRP
                     FVYRRYLDFSAIQSLREMKEKISREVVRRNLTDNIKFGAGGIREVEFIAQTFQMIRGG
                     RDKIL"
     gene            complement(1190..1471)
                     /locus_tag="PWY43_002259"
     CDS             complement(1190..1471)
                     /locus_tag="PWY43_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDL2137098.1"
                     /translation="MYYVIFAQDNPNMLEKRLEVRPAHLARLEKLQAEGRLLIAGPNP
                     TEDGSSVTGSTVIAEFNSLEEAQQWASQDPYVEAGVYGDVIIKPFKRVF"
     gene            complement(1473..1937)
                     /gene="yciA"
                     /locus_tag="PWY43_002260"
     CDS             complement(1473..1937)
                     /gene="yciA"
                     /locus_tag="PWY43_002260"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDL2137099.1"
                     /translation="MKDKPYGKAPEGKLILRTLAMPSDTNANGDIFGGWIMSQMDLGG
                     AILAKELAKGRVVTVTVDKMIFHRPISVGDVVCCYGKLMHIGRSSMQVKVEVFIKQVY
                     EGNRERYLVTEALFTYVAIDKEGKPRAVPRENNPELDEALSLIDLTPEKQGE"
     gene            complement(1927..2484)
                     /locus_tag="PWY43_002261"
     CDS             complement(1927..2484)
                     /locus_tag="PWY43_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDL2137100.1"
                     /translation="MKQLLEFIPLILFFVVYKMSGIQMASIALVIATIVQMIALKMLY
                     GKIEKQQIIMGGSVVFFGLLSAYFNEVKYLQWKVTIVYALFALVLLVSQFVFKTPLIK
                     KLLGKEIQLPEAVWNKINFGWAVFFLICMLLNIYISQYLSEEIWVDFKSFGIIGLTLV
                     ATVITGFVLYPYLKDAEKQEQKNER"
     gene            complement(2493..3302)
                     /locus_tag="PWY43_002262"
     CDS             complement(2493..3302)
                     /locus_tag="PWY43_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2137101.1"
                     /translation="MAQALLYFTTKYLQSIEGYFMSIKFVDLLQQSWNFMRNQQAFSL
                     FAIVTIVVVQLVFILVSPNSPELMVESQPQQLQIDASKMVSILLPTILLGVVNLLINV
                     LMIFNIQSINDGNYRQFFQNAGNALKHFLPVLLLQFVMVLPLSLGASFAMASPETVII
                     ALPVLVVGFYFFIKLSLVIYAYLLEKPQKSLSESIKFTLQLSRGKMLPLILFCVISYL
                     LPGMLSRLFTVFGNDFIGIFITIVLSAVINVFMAIFSFRFYQVYRQMPAVR"
     gene            3373..3810
                     /locus_tag="PWY43_002263"
     CDS             3373..3810
                     /locus_tag="PWY43_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDL2137102.1"
                     /translation="MLSLFDAGQRYLNTLPNQKKLANFMPDYHIIRLVKFSSKVMPAF
                     ACFALVWQYCFTDPTQSILANSVLTAVFAISIPFQGLYWLGKRARSPLSLSLLEWYES
                     LRQKLISAQVKLEETQAVPSFQDFANLLKLAEETWGDEYFDEL"
BASE COUNT         1261 a          824 c          712 g         1131 t
ORIGIN      
        1 gtaaaatttt atctcgcccg ccacggatca tttggaaagt ttgggcgata aactccactt
       61 cccgaatccc accggcacca aatttaatat tatcggttaa attacggcga accacttcac
      121 ggctgatttt ttctttcatt tcacgcaacg attggattgc actgaaatcc agataacggc
      181 ggtaaacaaa agggcggagc atttgcttta aatagcgatg atttaaattt tggctatcct
      241 cgcctaaaat ttttgcttta atcatcgcat aacgttccca atcacgccct tgctcttggt
      301 aataatcttc cattgcagta aaactcagca ccaaggctcc gctatcacca aaggggcgaa
      361 gtcgcatatc ggtacgatac acgaaaccat cagcggtgat ctcatctaat actttaatta
      421 accgctgccc cattcgggta aaaaatttgc tgttttcaat cggtttgcgt ccaccttcgg
      481 tttcaccaat atcggggtag gtaaaaatca ggtcaatatc ggaagaaaaa ttaagctctc
      541 gtccgcccaa tttgcccata cctaaaatca gcagttcttg tgtttcgcca agagtgttct
      601 ttggcgtgcc atattcggca cagcaacgct gaaacaacca atctcgagcg gctagaatca
      661 ggctctcggc taaatccgac aaatgctgaa ataccaattc taccgtcgct aatttatttg
      721 actgaataaa gctcagacga gccaattcac ggtggcgaaa ttgccgcaaa accctcccca
      781 acatttcttc attttccact cttgctaata tttttgctaa acgctcagta taattgcagc
      841 aatcatcagg cgtaggaaac ttggcaagcc attctgccag taaatccggc tgtttttgca
      901 aggtttgatg cacgaaattg gaaagcgaaa tcgccgtcac aagcggttga atttgctcag
      961 aattttgcaa attgggcggt aaattgagtt gggataaggg aattagggtg gtaagcataa
     1021 atttaggctc aaaattgaaa atagatagaa ttttacgctc gatcaattcc cctctttagc
     1081 aaagagggct taggggagat ttggcagagg taattttact ctcatacgaa acattaatat
     1141 cgtaatcaaa tctcccccag cccctctttt ctaaagaggg gagaaaaaat taaaacactc
     1201 ttttaaacgg tttaataatc acatcgccat acacaccggc ttccacataa ggatcttggc
     1261 ttgcccattg ttgagcttct tcaagtgagt tgaactctgc aattacagtt gaaccggtta
     1321 cgcttgagcc gtcttcagtc gggttaggcc ctgcgattaa taatcgacct tctgcctgta
     1381 atttttccaa acgagccaaa tgagccgggc gaacttctaa acgtttttcc aacatattgg
     1441 gattatcttg ggcaaagata acataataca tactattctc cttgtttttc aggggttaaa
     1501 tcaattagcg ataatgcttc gtctaattcg gggttatttt ctctcggcac agcacgcggt
     1561 ttgccttctt tatcaatcgc tacataggta aataaggctt cggttacgag ataacgttcg
     1621 cgattgccct cataaacttg tttgataaag acctcgactt tcacttgcat cgaagaacgc
     1681 cctatgtgca ttaacttgcc gtagcagcag accacatcac cgactgaaat cgggcggtgg
     1741 aaaatcattt tatctaccgt taccgtaacg actctacctt ttgccaactc tttggctaaa
     1801 attgcaccgc ctaaatccat ctgcgacata atccaaccgc cgaaaatatc gccattggcg
     1861 ttagtatctg acggcattgc caaagttctt aaaatcagtt tcccttccgg ggcttttcca
     1921 tagggtttat ctttcatttt tttgttcctg cttttcagca tcttttaagt aagggtaaag
     1981 gacgaaaccg gtaataaccg ttgcgactaa ggttaagcca ataatgccaa aggatttgaa
     2041 atcaacccaa atctcttccg ataaatattg actgatataa atattcagca gcatacaaat
     2101 cagaaagaat actgcccaac cgaagtttat tttattccac actgcttcag gcaactgaat
     2161 ttctttgcct aataattttt tgattaatgg tgttttgaac acaaattggc tcactaatag
     2221 caccaatgca aacagagcgt aaacaatggt tactttccat tgtaaatatt taacttcatt
     2281 aaaataggca ctcagtaagc cgaaaaagac caccgagcca cccataataa tttgctgttt
     2341 ttcaattttg ccgtagagca tttttaaggc gatcatttgc acgattgtag cgataaccaa
     2401 tgcgatagac gccatctgaa taccgctcat tttatacacg acaaaaaaca gaatcagcgg
     2461 aataaattca agtaactgtt tcattgatac tcctaacgca ctgccggcat ttggcggtaa
     2521 acttggtaaa agcggaagct gaaaatcgcc ataaagacgt taattaccgc acttaaaaca
     2581 atggtaatga atatgccaat aaagtcatta ccaaacactg tgaacaaacg agataacatc
     2641 cccggtaata aataagaaat cacacaaaat aaaatcagcg gtagcatttt tccacgtgat
     2701 aattgaagcg taaatttaat actttccgat agacttttct gcggtttctc caataaataa
     2761 gcataaatca ctaacgataa tttgataaaa aaataaaaac cgactaccaa caccggcaat
     2821 gcaataatca ctgtttcagg cgatgccatt gcaaaagatg caccgagaga aagcggtaac
     2881 accatcacaa attgtaacaa cagcacaggt aggaagtgct ttaatgcgtt tcctgcattt
     2941 tggaaaaatt gacgataatt gccatcgtta atcgattgga tattgaaaat cattaagaca
     3001 ttgatcaaaa gattgacaac acctaataaa atagttggca atagtataga caccatttta
     3061 gaggcatcaa tttgaagttg ttgcggttgc gactccacca ttagctcagg cgaatttggc
     3121 gacactaaaa taaaaaccaa ctgcacaacc acaatggtga caatggcaaa cagtgaaaat
     3181 gcttgttgat tacgcataaa gttccaactt tgctgcaata aatcgacaaa tttaattgac
     3241 ataaaatacc cttcaataga ttgtaaatat tttgttgtga agtatagcaa ggcttgagcc
     3301 aaactgcatt aacctattgt agattttaat cagttagcta aaataaaaga aaaatttgaa
     3361 agagataatc ctatgttaag cttatttgat gcaggacagc gttacctcaa cactctgccg
     3421 aatcagaaaa aattagccaa ttttatgcca gattaccaca ttattcgctt ggtaaaattt
     3481 tcctctaaag taatgcctgc ttttgcttgc tttgcattag tttggcaata ttgctttacc
     3541 gatccgaccc aatctatttt agccaattcg gtacttactg ctgttttcgc catttctatt
     3601 ccgtttcaag ggttatattg gcttggaaaa cgggcaagat caccgttatc tttatcatta
     3661 ttggaatggt atgagtcgct tagacaaaaa ttaatttcgg cacaagtcaa attagaagaa
     3721 acacaagcgg taccttcttt ccaagatttt gcaaatttac tcaagctagc agaagagact
     3781 tggggagatg aatattttga tgagttataa aataaaaaaa gccccactta aaataagtgg
     3841 ggtaaaactt ttgttctaat tctggaggtt tgaattactg ttttataaaa ggagacaaat
     3901 aaaacaagaa caaaagaaaa ataaaatc
//
LOCUS       DANKBV010000171         3866 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.171, whole genome shotgun sequence.
ACCESSION   DANKBV010000171 DANKBV010000000
VERSION     DANKBV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.171"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..297)
                     /locus_tag="PWY43_002264"
     CDS             complement(<1..297)
                     /locus_tag="PWY43_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2137103.1"
                     /translation="MFDTGSLDTLQALGVKIDGMPEFKVLSYLKPSADKAVNVGTVFE
                     PDLEALNSLNPDLIIVGTCTAKTFDEVSQIAKTIDMTDNGDKLIESGLQRLDSFG"
     gene            complement(258..422)
                     /locus_tag="PWY43_002265"
     CDS             complement(258..422)
                     /locus_tag="PWY43_002265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137104.1"
                     /translation="MIKKALFALTATAVFAASAFAKDVTIPTAERLLSKKPRLKLPCL
                     TPEVWILYKP"
     gene            596..2059
                     /locus_tag="PWY43_002266"
     CDS             596..2059
                     /locus_tag="PWY43_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2137105.1"
                     /translation="MAEYSGTTQEKMKDKQVEQINISPHSIEAEQAVLGGIMLSNDHW
                     DNVAERVQPHDFYNYAHRIIFEQMAELVRHNKPIDIITLDQALKDKGVLQDVGGFAYL
                     AELSKNTPSAANILAYADIVRDRSDLRSVISAGNQIAEMGYKTKGRNSKEVLDEAERI
                     VFEIAEKRNNANEGPQRIDDILLKTLARMDSLSKNRHNGGVTGVSTGFVDLNKKTAGL
                     QPSDLIIVAARPSMGKTTFAMNLCENASLVDIEDKDDLDEQGNPKKKPAKPVLIFSLE
                     MPADQIMMRMLASLSRVDQTKIRTGQISDDEDTAKISSTMAILQERNNIYIDDSSGLT
                     PTELRSRARRIYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEV
                     PVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAE
                     IIIGKQRNGPIGRVRLTFQGQYSRFDNYTGGHYDDEY"
     gene            2049..2354
                     /locus_tag="PWY43_002267"
     CDS             2049..2354
                     /locus_tag="PWY43_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDL2137106.1"
                     /translation="MNTKPTQPITLCAIYRSKAKEGMYLYVPKRDQFEQVPETLRQLF
                     GKPEFVMMFNLTGEKPLVRAKNEEVLQLLIEQGYYLQMPPPPENLHKAFIAQQKGEK"
     gene            2354..2803
                     /gene="nrdR"
                     /locus_tag="PWY43_002268"
     CDS             2354..2803
                     /gene="nrdR"
                     /locus_tag="PWY43_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDL2137107.1"
                     /translation="MRCPFCSTEETKVIDSRLAASGYQIRRRRECISCKERFTTFESA
                     ELVVPYVIKNNGSREPFDAEKLRTGLRRALNKRPVSADDVEKAISQIIIQLQAMGERE
                     VPSRHIGTLAMNALKQLDKVAYIRFASIYLSFSDIEEFIKEIEKLKE"
     gene            complement(2877..3746)
                     /locus_tag="PWY43_002269"
                     /pseudo
     CDS             complement(2877..3746)
                     /locus_tag="PWY43_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1177 a          784 c          843 g         1062 t
ORIGIN      
        1 gccgaaagaa tcaagacgct gtaatccgct ttcgattaat ttatcgccat tatccgtcat
       61 gtcgatggtt ttcgcaattt ggctgacttc atcaaatgtt tttgctgtgc atgtgccaac
      121 aatgatcaaa tccggattta aagaatttaa cgcttctaaa tccggctcaa ataccgtacc
      181 gacattcacc gctttgtctg cactcggttt aagataagaa agcaccttaa attcaggcat
      241 accatcaatt tttacaccta aggcttgtaa agtatccaaa cttccggtgt caaacacggc
      301 aattttagac ggggtttctt tgaaagtaac ctctctgcag ttggaatagt aacatctttg
      361 gcaaatgcag aagcagcaaa cacagcagtt gcggtcaatg caaacaaggc ttttttaatc
      421 attggataac tccatttata ttgaaaataa ttctcaaaga ttttaacgaa aattgatttt
      481 aagtaaagcg gaaaataaca agcgggcgaa ttttgcctaa aatttgcaaa tttcaattta
      541 attaaaattc cgatacacta tgtacatcaa tacgtcatta tagcaagaga aaaatatggc
      601 agaatatagc ggaacaaccc aagagaagat gaaagataag caggttgagc aaattaatat
      661 ctcaccacat tcgattgagg cagagcaagc ggtgcttggt ggcattatgc tcagtaatga
      721 tcactgggat aatgttgctg agcgtgtaca accgcacgat ttttacaact atgcgcatcg
      781 aattattttt gaacaaatgg cggagttggt gcgccacaat aaaccgattg atattattac
      841 gctggatcaa gccttaaaag acaaaggggt gctgcaagat gtaggaggtt ttgcctattt
      901 agccgagctt tctaaaaata ccccgagtgc agcaaatatt ttagcctatg cggatattgt
      961 gcgagatcgt tccgatttaa gaagtgtaat cagtgccggc aatcaaattg cggaaatggg
     1021 ctacaaaacg aaagggcgta actccaagga agtgttagac gaagcagagc gaattgtgtt
     1081 tgaaattgca gaaaaacgca ataatgccaa tgaagggcca caacgtattg acgatatttt
     1141 acttaaaaca ctcgcccgaa tggattcgct atctaaaaat cgccataacg gcggagttac
     1201 tggtgtttct accgggtttg ttgatctcaa taaaaaaacc gcagggttac agccttcgga
     1261 tttaatcatt gtggcagccc gtccttcaat ggggaaaact acttttgcaa tgaacttatg
     1321 tgaaaatgct tcattagtgg atattgaaga taaagatgat ttagacgaac agggtaatcc
     1381 gaagaaaaaa ccggcaaagc cagtgctaat tttcagcctg gagatgccgg cagaccaaat
     1441 tatgatgcgt atgcttgcct cactttctcg ggtcgatcaa accaagatcc gaaccggtca
     1501 gatttcagat gatgaagaca ctgctaaaat ctccagcaca atggcaattt tacaagaacg
     1561 taataatatc tatattgatg atagctccgg tttaacaccg accgaattac gttctcgagc
     1621 gagacggatt tatcgtgaaa atggcggatt aagcttgatt atggtggact atctccaatt
     1681 aatgcgagcg ccgggatttg cagataatcg tactttagaa atcgcagaaa tttcccgttc
     1741 tttaaaggca ctagcgaaag aattagaagt gccagtagtc gctctttctc agctaaaccg
     1801 gagtttggaa caacgtgccg ataaacgtcc ggtaaactcg gatttgcgtg agtcaggttc
     1861 tattgagcaa gatgcggact taattatgtt tatttatcgt gatgaggtct ataacgacaa
     1921 ttccgatctg aaaggcattg cggaaattat tatcggaaaa caacgtaacg gcccgattgg
     1981 tcgggtgcgt ttaacgttcc aagggcagta ttcccgtttt gataattaca caggagggca
     2041 ttatgatgat gaatactaaa ccaactcagc caatcacact ttgtgcgatt tatcgcagta
     2101 aggcaaaaga aggaatgtat ttatatgtgc ctaagcgtga ccaatttgag caagtaccgg
     2161 aaacattacg ccagttattt ggtaagcctg aatttgtgat gatgtttaat ttaaccggtg
     2221 aaaagccatt ggtgcgtgct aaaaacgaag aagtgttaca attacttata gagcaaggct
     2281 actatttgca aatgccacct ccgccggaga atttgcataa agcctttatc gcacaacaga
     2341 aaggggaaaa ataatgcgct gcccattttg ttccactgaa gaaactaaag tgatcgattc
     2401 acggcttgca gcaagcggtt atcaaattcg ccgccgccgt gaatgtataa gctgtaaaga
     2461 acgttttact acctttgaat cggccgaatt agttgtgcct tacgtgatca aaaataatgg
     2521 tagccgagag ccgtttgatg ccgaaaaatt aagaacaggg ttaagacgtg cattaaataa
     2581 acgccctgtc agtgctgatg atgtcgaaaa agcaattagc caaatcatta ttcagctgca
     2641 agcgatgggc gaacgagaag tgccgagtcg ccatatcggt acattggcaa tgaacgcact
     2701 taaacaatta gacaaagtag catatatccg ctttgcctct atttatttaa gctttagtga
     2761 tattgaagag ttcattaaag aaattgaaaa attaaaagag tagttgcaaa aaaacataaa
     2821 aaaatgaccg cttgtgagtt taaaatccac aagcggtcat ttttctttaa aaatactcaa
     2881 ttagctttta cttgttccaa cacaaaccct acaaacgccc gcacaatgct agacggataa
     2941 cgatgggcgg cataaatcat cgtcaaatca aatttggggt tttctatctc gcccaagact
     3001 tccaccaagc cattttctgc cacgagtcta tccaccatgc tgtcggcaag cagcgccact
     3061 gctttgccct gttttgccat atattcaatg gcatagctgt cattgctggc aaaaagatag
     3121 ggaatttcca ccgcttgtct gccagtgcca agtgtaatga cgcttgcttc attttttgcc
     3181 caagcggcaa tggggaaatt tttcaaatct ttgatggtat tgggcgtacc caatcgtgcc
     3241 aataaatcag ggtgtgccac ccatttactg ccgatttgcc ccactttttt ggcgataaac
     3301 tgttcgccgt gtaactgccc aatccgaaat gctacatcaa tgccgtctgc cgacaaatca
     3361 agcagacggt cggtcagcgt gcaatgcacc tgtacctgtg ggtaggctgc ctgaaaatca
     3421 gctatccacg ccaaaacagg ctcgcaggca gggcttgccg acaggcgtaa tttgcccttt
     3481 aaattgcctt gctcttcaaa caacacttct ttggcagtgg caagctgttc aatggacaaa
     3541 tgcacctgtt catacaggcg ttgccctgcc attgtgggtt taacgcctga tttttagtgg
     3601 tcaaagagtt gaatgttaag ctctttttcc aaatcggcaa tttgacggct aatggttgca
     3661 atcggtacat tgagtcgctc ggaggctttg gagagactgc cgtgctggat aacggcgaca
     3721 aagaggcgga gggtgttgag gttcattgtt tttctcccca atgttcaatt tcttgaataa
     3781 aatgtttggg gctgtagtag attagcccta aatctcacac cattttcgca acgttttgag
     3841 ctgttctttt ggtgtaccaa agttaa
//
LOCUS       DANKBV010000172         3800 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.172, whole genome shotgun sequence.
ACCESSION   DANKBV010000172 DANKBV010000000
VERSION     DANKBV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.172"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(303..1376)
                     /locus_tag="PWY43_002270"
                     /pseudo
     CDS             complement(303..1376)
                     /locus_tag="PWY43_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            1530..2708
                     /gene="lpxB"
                     /locus_tag="PWY43_002271"
     CDS             1530..2708
                     /gene="lpxB"
                     /locus_tag="PWY43_002271"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDL2137108.1"
                     /translation="MKQQFPVIGLVAGEVSGDILGAGLIKALKVHYPNARFIGVTGSR
                     MIEAGCQSLFDMEELSVMGLAEVVKHLPRLLKRRKQVIDEMLKLKPDVFIGIDAPDFN
                     LTVEEKLKASGIKTIHYVSPSVWAWRQKRVFKIARAANLVLAFLPFEKAFYDKFNVPC
                     RFIGHTMADAIPLEPNRTEACLALNIDESKRYMAILVGSRGSEIQFLAEPFLKAAQLL
                     KARFPDLQFLVPMVSQKRMEQFNAIKQQVAPELELNIIQGKARQAMIASECTLLASGT
                     AALEAMLCKSPMVVGYRMKPLTYWLAKKLVKTDYISLPNLLAQAPLVPELIQEACNPE
                     NLAQELSLFLADDEKNQQHKALLKQQFMQLHQSIQCNADEQAAQAVIDLLNGSTSGQI
                     "
     gene            2735..3787
                     /gene="sohB"
                     /locus_tag="PWY43_002272"
     CDS             2735..3787
                     /gene="sohB"
                     /locus_tag="PWY43_002272"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDL2137109.1"
                     /translation="MWKEVLLNYGIFLLELLTVFGIIAVVVMLILESKKQNENGSISI
                     TNLSEKYKEQQKSLSGFFLSEEELKHQEKQEKKVAKEKAKAEKKRLKAGEECVESPSR
                     LFVLNFNGDMMAHEVNSLRREIDAVISLANPEKDEVLLKLESPGGVVHGYGLAASQLQ
                     RLRDRNIYLTAAVDKVAASGGYMMACVANKIVSAPFAVIGSVGVVAQVPNIHRLLKKH
                     DIDVDVMTAGEYKRTVTLVGENTEKGKQKFQQELEETHSLFKQFVARRRPQLDIEQVA
                     TGEHWFGQQAIELNLVDEIATSDDLLLNAIKEKEVIELKYKEKKKLAQKLGSQFEQSA
                     ENLLAKILHKSRSTLM"
BASE COUNT         1123 a          662 c          833 g         1182 t
ORIGIN      
        1 taataacccc tcatcacttt ttgcagttta acctcttttg caacagataa tttaaattta
       61 ggcgttttgg ctgctcagtt attgccatta tgattatatt agttcacact aaaattttag
      121 tgttgtttct taaagctgaa gatgctaata ggtgagtaag cggtttttac cactttgaag
      181 ataaatgcaa tagcttcttg tttgaaggat tagttgtatt taatcataaa aaatctgcac
      241 cttaatcagt tggttagccc aacttttagg tgcagatcat tctagtacgc ttttcggttt
      301 tattaccaat atgctcgtaa gcctacaaca gtcgtataat tgcgagattt tgcggttttt
      361 acatcattag tatagtgttt agcacgagtt gcttgccatt caacaaagcc ttttaagtgt
      421 tttggaacaa tgtaatattc cgttcccaac ataaattggt ttcttttaac tttgctatca
      481 tttgccaagc ctacagcatt aagtttgttt tctttataag catacattgt gtaagcattc
      541 cattgatcgg ttaaacgatg gagtaacaca gtgcgaactt ctttttcgat acgtttgtga
      601 ccgatagcat tttgatcttc cgtttcacgg cgttctaaat ctaagccaag ggtagttttt
      661 ccgtaggtat aagctgtacc gaaggcaaga gcagtgcgtt cataattgcc gatagattgc
      721 tcagaacgct cacgggttgc acctgttgct aattttaagc tttggttatc atcaatttta
      781 tagttataaa ttgctgctgc accccaaata tctttacgtt tattggctaa atctaagcca
      841 ttgtttccac gttggctatt tccgccatag aatgctccga gttttaatcc ttcgatgcct
      901 ttgtaaacat attaaagcgt acgtcgtgat gaaccggtaa gtaagccatc acttaagctg
      961 agtgtatttg gtaagtcagt ttgttttact tcatcggcaa tattagtcat attgccgtat
     1021 gttaattcac cgtattgttt atgcccgatt cctgcataga gttgatgatc ataaatatga
     1081 tcgaagttat gttgtgagct tgaatcgcca cgcatacgcc attccacacg acctaaagca
     1141 tagaatccgt gacccacttg ctgggttaaa cggaaaccaa aacgagagcc gttgttatcc
     1201 actgcgtgat taacgtggtt gcgggtagct ttaccattta ccggtgaggt tttatctgag
     1261 gtgctttccc acttaatacg agcagaacca ataaattcaa tttgtgtacc ggtttcttta
     1321 tgatcgtaaa tcgtaatagc tagagctgag tttgcgatta aggctgatgc taaaatcgct
     1381 catgttgttt tttcataaaa atcactccat atgaatagat aaagggaggt agtgtatgtg
     1441 aatattcatt gctagacaat aatgttttgt tagtttatat gatgattggt tataatagag
     1501 tcacttacag atcttcaata atatttaata tgaaacaaca attccccgtt attggcttag
     1561 ttgctggtga ggtctctggc gatattcttg gtgccgggtt aattaaggcg ttaaaagtac
     1621 attacccgaa tgctcgattt atcggtgtta caggctctcg aatgattgag gctggctgcc
     1681 agtctttgtt tgatatggaa gagctttctg taatggggct agcagaggtg gttaaacatt
     1741 tacctcgttt acttaagcgt cgtaaacagg ttattgatga gatgcttaaa ttgaaaccag
     1801 atgtatttat cggtattgat gcaccggatt ttaatttaac cgttgaagaa aaactcaaag
     1861 cgagtgggat taaaacaatc cattatgtta gcccttcggt gtgggcttgg cgacaaaagc
     1921 gggtgtttaa aattgccaga gcagctaatt tagtgttggc atttctgcca tttgagaaag
     1981 cgttctatga taaattcaat gtgccttgtc gttttatcgg gcatacgatg gcagatgcga
     2041 tcccactcga accaaatcgt acagaggcat gtttagcgtt aaatattgat gaatccaagc
     2101 gatatatggc aattttggtg ggaagccgtg gcagtgaaat tcagtttttg gctgagcctt
     2161 ttttgaaggc tgctcagttg ttaaaagccc gttttccgga tttacagttt ttagtgccaa
     2221 tggtcagcca aaagcgaatg gagcaattta atgcaattaa acagcaagta gctcccgagt
     2281 tggaactgaa tattattcaa ggcaaggctc gtcaggctat gattgcgtca gaatgtactc
     2341 ttttggcatc gggaacagca gcactagagg caatgttgtg caaatctccg atggtagttg
     2401 gttatagaat gaaaccgctg acttattggt tagcaaaaaa attagtgaaa accgattata
     2461 tctccttacc gaatttattg gctcaagccc cattggtacc agaattgatc caagaagcat
     2521 gcaacccgga aaatttagct caagagttat ctctattttt agctgatgat gagaaaaacc
     2581 aacaacataa agcattgcta aaacagcagt ttatgcagct acatcaatca attcaatgta
     2641 atgctgatga gcaagctgcc caagcggtta tcgatctcct aaacggctcg acaagcggtc
     2701 aaatttagtt agttttttgt aaaaaggaaa atttatgtgg aaagaagtat tacttaatta
     2761 tggtattttt ttattagagt tattaactgt atttggcatt attgcagtgg tggtgatgct
     2821 gattttggaa tcgaaaaagc agaatgaaaa cggctcaatt tcaattacca atttatctga
     2881 gaaatataaa gagcagcaaa aatctctttc cggctttttt ctaagtgaag aagaattaaa
     2941 acatcaggaa aagcaggaga aaaaagtagc aaaagagaaa gctaaagcag aaaaaaaacg
     3001 cttaaaagca ggcgaagaat gtgtagaaag cccttcccgt ttatttgtat tgaattttaa
     3061 tggcgatatg atggctcacg aagtaaattc cctacgccgt gaaattgatg cggtaattag
     3121 cctcgcgaat ccggaaaaag atgaagtttt acttaaactt gaaagtccgg gcggagtggt
     3181 gcacggttat ggattggctg cttcgcagtt acaacgtttg cgtgatcgca atatttattt
     3241 aaccgcagcg gtagataaag tagctgccag cggtggttat atgatggcgt gtgtggcgaa
     3301 taaaattgtc tctgccccat ttgcggtgat tggctcagtg ggggtggtgg ctcaggtgcc
     3361 gaatatccac cgcttactga aaaaacacga tattgatgtg gatgtgatga ctgcaggtga
     3421 atataaacgc acggtgacgc tggtgggaga aaataccgaa aaagggaaac agaaattcca
     3481 gcaagaactg gaagaaacgc actctctgtt taagcaattt gtggctcgac gccgcccaca
     3541 actggatatt gagcaggttg caacaggcga gcattggttt ggtcagcaag cgattgagct
     3601 aaatttggtt gatgagattg caaccagtga tgatctgctc ctcaatgcaa ttaaggaaaa
     3661 agaagtgatc gagctaaaat ataaagagaa gaaaaaactt gctcagaaat tgggttctca
     3721 attcgagcaa tcagcagaga acctgctcgc taagatttta cataaaagtc gttcaacgct
     3781 gatgtagcgt atatattatt
//
LOCUS       DANKBV010000173         3787 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.173, whole genome shotgun sequence.
ACCESSION   DANKBV010000173 DANKBV010000000
VERSION     DANKBV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.173"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            40..771
                     /locus_tag="PWY43_002273"
     CDS             40..771
                     /locus_tag="PWY43_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDL2137110.1"
                     /translation="MFDWITNPEAWVALFTLTGLEIVLGIDNIIVISILVSRLPIEQR
                     QSARIIGLGLAMGTRILLLLSLAWMMRLVEPLFTIGNNPISGRDLILLIGGLFLIVKS
                     AMELKESISAESHEEKSESIKKASFFMVLIQIAIFDIIFSLDSVITAVAMADDIPVMV
                     IAIIIAVAVMMLAAKTIGDFVDNNPTIKNLALAFLILIGIVLVGEGFDIHIPKAVVYT
                     AMGFSVVVEMLNIKMRRNQAKLEQA"
     gene            897..1934
                     /gene="nagZ"
                     /locus_tag="PWY43_002274"
     CDS             897..1934
                     /gene="nagZ"
                     /locus_tag="PWY43_002274"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005711660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDL2137111.1"
                     /translation="MLLIDIKDKELTQEEVEMLEHPLVSGLILFTRNFYDKAQIQALV
                     KSIRQRVKKPLLITVDQEGGRVQRFREGFTKLPAMQSFSLLAQTQEHALQLARETGWL
                     MAAEMFALDIDLSFAPVLDLGHECKAIGDRSFGKDPYTILPIAEAFIDGMLEMGMATT
                     GKHFPGHGYVLADSHLETPFDDRAKEVIFNHDILPFKQLISKGKLSAIMPAHVVYTEC
                     DNQPASGSIYWLKDILRKQLNFNGVIFSDDLGMRGASFMGNVVERTEKAIQAGCDLLL
                     LCNQPEGVVQVLDGLKYQPTQIQKARHLSLMKRKTVTWRELENSKAYQQAQARLTALQ
                     NHWLAWKETNA"
     gene            2059..2640
                     /locus_tag="PWY43_002275"
     CDS             2059..2640
                     /locus_tag="PWY43_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2137112.1"
                     /translation="MQNKTEILPLVASPISHFRNKAKMVVSGSVERPILGILKDQHDP
                     QSGVDLTDCLLYPASFYSLFPILKDFIGRAGLVPYNIKKKKGELKYILITESQYNQSL
                     MLRFVVRSESKRALVERELPHLLEKLPPNSVVSLNIQPQHAAVLEGETEIFLTERKVI
                     EENFNSVPLFIRPQGFFQTNPTVASQLYLTAQQ"
     gene            2890..3438
                     /locus_tag="PWY43_002276"
                     /pseudo
     CDS             2890..3438
                     /locus_tag="PWY43_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
     gene            3608..3787
                     /gene="rumB"
                     /locus_tag="PWY43_002277"
                     /pseudo
     CDS             3608..3787
                     /gene="rumB"
                     /locus_tag="PWY43_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617603.1"
                     /note="RNA uridine methyltransferase B; catalyzes the
                     formation of 5-methyl-uridine at position 747 in 23S rRNA;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase"
BASE COUNT         1170 a          767 c          745 g         1105 t
ORIGIN      
        1 aaatatcact taatccctta tttttaaaag gacattttta tgtttgactg gatcacaaat
       61 cctgaagctt gggttgcgct ctttacactt accgggcttg aaatcgtatt aggcattgat
      121 aacatcatcg tcatcagtat tctcgtttcc cgtttaccta ttgaacaacg ccaatctgcc
      181 cgaattatcg gcttggggct ggcaatggga acccgtattt tattgcttct ctcgcttgct
      241 tggatgatgc gtttggtcga gcctcttttc actattggca acaatccaat ttcaggtaga
      301 gatctcattc tactcatcgg tggattattc ctgattgtta aaagtgcaat ggagctgaaa
      361 gaatccattt ctgccgaatc tcacgaagaa aaaagcgaat ccattaaaaa agccagcttt
      421 tttatggtgt taattcaaat tgcaattttt gatattattt tctcccttga ttcagtcatt
      481 actgctgtgg caatggcaga tgatattccg gtaatggtta ttgcgattat cattgcagtt
      541 gcggtaatga tgttagcagc gaaaaccatt ggggactttg tagataacaa cccaactatt
      601 aaaaatctcg ctttagcgtt cttgatttta atcggtattg tattagtcgg cgaaggtttt
      661 gatattcata ttccaaaagc agtggtttat acggcaatgg gcttctcggt tgtggttgaa
      721 atgctcaaca taaaaatgcg tcgtaaccaa gctaaattag aacaagcata attgctctgt
      781 ctcatttatg cccttaatca ttcgcccttg atcaacatca agggcttttt aatgtagaat
      841 aggcacaaca agcggtcata tctgctcatt tttttgcaaa tagctcaacg aaaattatgt
      901 tactgattga tattaaagat aaagaactga ctcaagaaga agtcgaaatg ctggaacacc
      961 ctttagtttc cgggctaatt ttattcaccc gtaatttcta cgacaaagca caaattcagg
     1021 cattagtgaa atccattcgc cagcgggtga aaaaaccgtt gcttattact gtcgaccaag
     1081 aaggcggtcg ggtgcaacgc ttccgtgaag gctttacaaa attgcctgca atgcagtctt
     1141 ttagcctgct agctcaaacc caagagcacg cattgcaact cgcgagagaa acaggctggt
     1201 taatggcggc tgaaatgttt gcattggata ttgatttaag ttttgcccct gttttagatt
     1261 taggacacga atgcaaggca attggcgatc gatcatttgg gaaagatcca tacacgattt
     1321 tgccaattgc tgaagccttt attgacggaa tgttggaaat ggggatggca actacaggca
     1381 agcattttcc tggtcacgga tatgttttag ctgattcaca tttagaaacg ccttttgatg
     1441 atagagcaaa agaggtgata tttaatcacg atattctccc cttcaaacaa cttattagta
     1501 aaggtaaact ctccgctatt atgccagctc acgttgttta taccgaatgc gataaccagc
     1561 ctgcaagcgg ttcaatttac tggttaaaag acattttacg caaacaactc aatttcaatg
     1621 gtgtcatttt ctctgatgat ttgggtatgc gaggtgctag ctttatgggt aatgttgtag
     1681 aacgcaccga aaaagccatt caagccggct gcgatttatt gctgctttgc aatcagccag
     1741 agggcgtcgt tcaggtttta gatggcttga aatatcagcc tactcaaatt caaaaagcgc
     1801 gtcatttatc attaatgaag cgtaaaactg tcacttggcg tgagctagaa aacagtaaag
     1861 cttatcagca agctcaagct aggctgaccg ctttacaaaa tcactggcta gcgtggaaag
     1921 aaacaaatgc ctaatctagc taactgctca cattttaaac gtggtgattg cacctcctgc
     1981 caatgacttg aaaaaccata ccagagccaa ttagccgata aagaagccga cctcaagcgg
     2041 ttaatttctc catttatttt gcaaaataaa acggaaatct taccgcttgt agcatccccc
     2101 atttcacatt ttcgcaataa agcgaaaatg gtggtttcag gtagtgtgga acgcccaatt
     2161 ttaggcattt taaaagatca acacgacccg caaagtgggg tagatttaac cgactgccta
     2221 ctttacccag cttcttttta tagcttattc cccattctta aagattttat tggccgagca
     2281 ggccttgtgc cctacaacat caaaaagaaa aaaggcgagc tgaaatatat cctaatcact
     2341 gaaagccaat ataaccaatc gttaatgctc cgttttgtgg tgcgaagcga atcaaagcga
     2401 gcattggtgg agcgagaatt accccatctg ttagaaaaat taccgcctaa ttcggtggtt
     2461 agcctaaata ttcaaccgca acacgccgct gttttagaag gtgaaacgga gattttctta
     2521 accgaacgaa aagtgatcga ggaaaatttc aatagcgtac cgctatttat tcgcccacaa
     2581 ggctttttcc aaacaaatcc aactgtagcc agccagcttt acttgacagc tcaacaatga
     2641 gttgaaaatt taccaataaa aaagtgttgg gatctgtttt gtggcgtagg cggttttggt
     2701 ttacattgta caaaagcatt acaaaaaaaa ggattgatgt tgaattaact ggcattgaaa
     2761 tttcagcatc cgcaattgaa agtgccacaa aatcagcggc aacattagcc ttagaacttg
     2821 acaaaatttg actgctatgg ttaaaatgaa ttgccaatta acggcttatc tatgaaaaag
     2881 ggcagtctta tgaaaatgaa aacagcatta gcaattgcat taggttcttt attcagcttt
     2941 tcggtaacag cagccgaact ggaaaaaatt accgtgccga ttcaacttct tgatgtagaa
     3001 aaaggcaata aagatattgg tacagtagaa attactgaat ctaaataatc taaatacggc
     3061 ttagtattta ctccaaatct aaaagattta acagaaggct tacacggctt ccatattcac
     3121 gaaaacccaa gctgtgatgc aaaagaaaaa ggtggcaagc tcacagcagg cttagccgca
     3181 ggaggacact ggaaccctaa taaagcccca catcacggct tcccgtggtc tgatgatgct
     3241 catttaggcg atttacctgc attaaccgtt ttacacgatg gtacatcaac taatccagta
     3301 ttagcccctc gcttgaaaaa attagatgaa attaaaggtc gttcattaat gatccacgaa
     3361 ggcggcgata accactcaga ccacccggct cctcttggtg gtggtggtcc tcgaatggcg
     3421 tgtggtgtaa ttaaataaaa tttgatctag ttagaaagtg ttggtttaaa tatcattcca
     3481 acactttttt atttctagcc attaccatat aattgcctcc tattaaggag aaatatatgt
     3541 ctataaaagc aaaaaaagaa ccccataaaa tacttccaga acaagcaact ttttctaaga
     3601 gttcagagta agatttatct cgctcgattc tgcaaaattt gccttaaatg aaaacagcac
     3661 cccacctgat ttggtgattg taaacccacc tcgtagaggc attggtcaat ctttagcgga
     3721 atttttaaac aatttgggct caacttattt aatctattcc agctgcaatg cccaatctat
     3781 ggcaaaa
//
LOCUS       DANKBV010000174         3735 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.174, whole genome shotgun sequence.
ACCESSION   DANKBV010000174 DANKBV010000000
VERSION     DANKBV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.174"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..484
                     /locus_tag="PWY43_002278"
     CDS             <1..484
                     /locus_tag="PWY43_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HDL2137113.1"
                     /translation="KGWVLSKVEKSTVGENQIQQAKQPDQVWQTSLTPSKLGIVSLKP
                     ESLSISGLADYVGFLKDTGQDPKRFEITFWRKVFQPISMAVMMLLAISFIFGPLRSST
                     MGAKIVIGIVAGFMFYVANIVFGNLSLIATWLPVPVGALIPSLLCLIIVWWLLSKKRD
                     "
     gene            710..>3735
                     /gene="dnaE"
                     /locus_tag="PWY43_002279"
     CDS             710..>3735
                     /gene="dnaE"
                     /locus_tag="PWY43_002279"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL2137114.1"
                     /translation="MSDCRFVHLKVHSDFSMINGLAKVKSLVKTAVANNMVAMGLTDF
                     TNFCGLVKFYGEALGAGIKPIIGADVYVRTEAGSEDYFELTLLAKNNKGYHNITQILS
                     KAYQQGYIEFPMVEREWLAELNEGIIILSGGRMGDVGKALLKENRQEAEEKVAFYQQY
                     YPNHFYLSVCRTGRADEERYIKQAVEFSQKNAIPLVAVNDVVFLKEDDFDAHEIRVAI
                     HDSYTLDDPKRPKKYSPKQYFRTEEEMCKLFADLPSALANTVEIAKRCSVTVRLGEYF
                     LPNFPTGELSTEDFLVKKSKEGLEERLEFLFPDPEERANKRSVYDERLQVELDVINQM
                     GFPGYFLIVMEFIQWSKDNDIPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLN
                     PERVSMPDFDVDFCMDGRDRVIEHVAETYGRQAVSQIITFGTMAAKAVIRDVGRVLGH
                     PYSFVDRISKLIPPDPGMTLEKAFAAEPKLPELYEADEEVKDLIDMARKLEGVTRNAG
                     KHAGGVVIAPTAITDFSPLYCDSEGLHPVTHFDKNDVEYAGLVKFDFLGLRTLTIIKW
                     ALEMINERLAREGKEPIRIETISLDDKKSFALLLAAKTTAVFQLESRGMKDLISRLKP
                     DCFEDIIALVALFRPGPLESGMVQNFIDRKHGKEEVSYPDPQYQHECLKPILEPTYGV
                     IVYQEQVMQIAQELAGYTLGGADLLRRAMGKKKPEEMAAQREVFEKGAEAKGIDGKLA
                     GQIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTSEMDNTDKIVG
                     FYDECMNMGLTVVPPDVNSGKHRFSVNEKGEIVYGLGAIKGVGEGPIEAILAARQKEG
                     KFKDLFDLTARVDLKKINRRTFESLIMSGAFDKLGPHRAALMKNLEDALKASDQHAKM
                     EELGQSDMFGVLTETPEEVQNAYANTPKWSEQVILDGERATLGLYLSGHPIGRFLKEL
                     SHYAPNRLNELQPTFRGQMTTVSGIVMASRVAVTKRG"
BASE COUNT         1037 a          745 c          940 g         1013 t
ORIGIN      
        1 aaaaggctgg gtgttgtcta aagtggaaaa atcgactgtg ggagaaaatc agatccagca
       61 agctaaacag ccggatcaag tttggcaaac cagtttaacc cctagcaaat taggcattgt
      121 ttcactcaaa ccggagtcgc tctcgatttc aggcttggca gattatgtcg gcttcttaaa
      181 agataccgga caagatccaa aacgttttga gatcaccttc tggcgaaaag tattccaacc
      241 aatctcaatg gcggtgatga tgctacttgc gatctccttt attttcgggc cgcttcgtag
      301 tagtacgatg ggggcgaaaa ttgtgattgg tattgttgcc ggctttatgt tctatgtggc
      361 aaatattgtg tttggcaatt taagcttaat tgccacttgg ttacctgtac cggttggagc
      421 attaattcca agcctactct gtttgatcat cgtttggtgg ctgttaagca aaaaacgtga
      481 ttaattttta ctcccctctt tagataaagc agggttgtca ggttctgaaa gggcgaatgt
      541 aattcgcccc tacattcgga ttttaaatgt aactctgagc aagaataatg ctcgtttgta
      601 ggggcgaata acattcgccc aacgggattt tattcacagc aggcttcact ttttagaacc
      661 tgacagtctt tactaaagag gggaactgct ttacttagat tatattccta tgtcagattg
      721 tcgtttcgtt catttaaaag ttcacagtga tttctcaatg attaatggtt tagctaaggt
      781 taaatcgtta gtcaaaacgg cggttgccaa taatatggtg gcgatgggct tgacggattt
      841 tactaatttt tgcgggttgg tgaaatttta tggcgaagca ttgggagctg gaattaagcc
      901 gattatcggg gcggatgttt atgtgagaac cgaggcaggc agtgaagatt attttgaact
      961 gacattactt gcaaagaaca ataaaggcta ccataacatt acccaaattt tgtctaaggc
     1021 ctatcagcaa ggctatattg aatttccgat ggtggaacgt gaatggctgg cagagttgaa
     1081 tgaaggcatt atcattcttt cgggcgggcg aatgggcgat gtgggtaaag ccttactcaa
     1141 agaaaaccga caagaagctg aagaaaaagt ggctttttat cagcaatatt acccgaatca
     1201 tttttacctt tccgtttgcc gtaccggacg ggcagacgaa gaacgctata tcaaacaagc
     1261 ggtcgaattt tcacaaaaaa atgcaattcc gttagtggcg gtaaatgatg ttgtcttctt
     1321 gaaagaagat gactttgacg cacacgaaat tcgggttgcc attcacgaca gctacaccct
     1381 agacgacccg aaacgcccga aaaaatactc accgaaacaa tatttccgta ctgaagagga
     1441 aatgtgcaag ctgtttgccg atttaccgag tgcattggca aacacggttg aaattgcgaa
     1501 acgctgttcg gttaccgtgc gtttgggcga gtatttcctg ccaaatttcc cgaccggtga
     1561 gctttccacc gaagatttct tggtaaaaaa atcgaaagaa ggcttagaag agcgtttaga
     1621 gtttctattc cctgatcctg aggaaagggc gaacaagcgg tcggtttatg acgaaagatt
     1681 gcaagttgaa ttagatgtga tcaaccaaat ggggttcccg ggttactttt tgatcgtgat
     1741 ggagtttatc caatggtcca aagataatga tattccggta ggaccgggtc gtggctcagg
     1801 ggcaggctca ttggtggctt atgcgttgaa gattaccgat cttgacccat tggaatttga
     1861 tttgcttttt gagcgttttc tcaaccctga gcgtgtttca atgcccgatt ttgacgttga
     1921 tttctgtatg gacgggcgag accgtgtgat tgaacacgtt gcggaaacct acgggcgaca
     1981 agcggtatcg caaattatca cattcggtac tatggcggcg aaagcggtca ttcgagatgt
     2041 agggcgagtg cttggacacc cctatagctt tgtggatcgc atctccaaac tcattccgcc
     2101 cgatccagga atgacgcttg aaaaagcctt tgccgctgag ccaaaattgc ctgagctata
     2161 tgaagccgat gaagaggtga aagaccttat tgatatggct cgtaagctcg aaggtgtaac
     2221 tcgtaatgca ggtaaacacg ctggtggtgt ggtgatcgcc ccaactgcga ttaccgattt
     2281 ttcgccgctt tattgcgata gcgagggctt acacccggtt acccattttg ataaaaatga
     2341 cgtggaatat gcaggattgg tgaagtttga cttcttaggc ttgcgtacgc tcaccatcat
     2401 caaatgggcg ttggagatga ttaacgaacg tttggctcgt gaaggcaaag agccgattcg
     2461 gattgaaacc atttcgcttg atgataaaaa atcctttgct ctgttgctgg cagctaaaac
     2521 tacagcagta ttccagcttg aatcacgggg aatgaaagac ttaatttctc gcctgaagcc
     2581 ggactgtttt gaagatatta tcgcattggt ggcactattc cgcccagggc cgctggaatc
     2641 aggtatggtg cagaacttta ttgaccgtaa acacggcaaa gaagaggttt cttatcctga
     2701 tccgcaatac caacacgaat gcttaaaacc tattctcgag ccgacttatg gggtcattgt
     2761 ttaccaagaa caggttatgc aaattgcaca agagcttgcc ggttatacac tcggtggagc
     2821 ggacttactc cgccgtgcaa tgggtaagaa gaaaccggag gaaatggctg cacagcgtga
     2881 agtgtttgaa aaaggggcgg aagccaaagg gattgacggt aaacttgccg gtcaaatttt
     2941 tgacttggta gaaaagtttg ccggctacgg ctttaataaa tctcactcag cagcttatgc
     3001 gttggtttct taccaaacac tttggctaaa agcccattat ccggcagaat ttatggctgc
     3061 ggtaatgacc tcagagatgg ataataccga taaaattgtc gggttctacg atgagtgtat
     3121 gaatatgggc ttaaccgttg tgccgcctga tgtaaacagc ggtaaacatc gttttagcgt
     3181 aaatgaaaaa ggcgaaattg tttacgggct aggagcaatt aaaggcgttg gggaaggacc
     3241 gattgaggca attctggcag ctcgccaaaa agaggggaaa tttaaagatc tatttgattt
     3301 aaccgctcgg gtcgatctga aaaaaatcaa ccgccgcacc tttgaaagcc tgattatgtc
     3361 cggtgctttt gacaaattag gccctcaccg agctgccttg atgaagaatt tggaagatgc
     3421 acttaaagcc tcagatcagc acgctaaaat ggaggaactc gggcagagcg atatgtttgg
     3481 cgtgcttacc gagacaccgg aagaagtgca aaatgcttac gccaatacgc caaaatggtc
     3541 ggagcaagtg attttagatg gagagcgtgc cacgctcggg ctgtatttaa gcggacaccc
     3601 gattggtaga ttcctcaaag aattatccca ctatgcccct aatcggctga atgaattgca
     3661 accgactttc cgagggcaaa tgaccacggt ttcaggaatt gttatggcat ctcgagtggc
     3721 agttaccaag cgagg
//
LOCUS       DANKBV010000175         3680 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.175, whole genome shotgun sequence.
ACCESSION   DANKBV010000175 DANKBV010000000
VERSION     DANKBV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.175"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1331)
                     /gene="srmB"
                     /locus_tag="PWY43_002280"
     CDS             complement(<1..1331)
                     /gene="srmB"
                     /locus_tag="PWY43_002280"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDL2137115.1"
                     /translation="MTQLMTFEELDLSPQLLKALAHKGYNRPTAIQQATIPAALEGRD
                     LLGSAPTGTGKTAAFLLPAIQHLLDYPRCKPGAPRILILTPTRELAMQVAEQAQALAK
                     FTQLSIATITGGVAYQNHGEVFNKNQDIVVATPGRLMQYIKEENFDCRAVEVLIFDEA
                     DRMLQMGFGQDAEKIAAETRWRKHTWLFSATLEGELLEDFAERLLNDPIKVDAEPSRR
                     ERKKIQQWYYHADNVEHKTKLLARIISEFSVERAIVFVRRREDVRELSETLRKRGIRS
                     TYLEGEMAQTQRNQAINRLKEGVVNVLVATDVAARGIDIDDIDFVLNYDLPYSADTYL
                     HRIGRTARAGKKGSAISLVEAHDYKLLGKIKRYTEEVLKPRIIEGLEPRTKAPKDGEL
                     NVTSKKEKARIKKKKEAKKEAAKPKVKVRHKDTKNIGKRRKPSSESAAVKQA"
     gene            1491..1754
                     /locus_tag="PWY43_002281"
     CDS             1491..1754
                     /locus_tag="PWY43_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDL2137116.1"
                     /translation="MFVEIYGRLSCPYCVRAKTLAEKMKQELSDFDFKFIDMIAENIS
                     KQDLEPRVGKPVATVPQIFVDNNHVGGCTDFQAFVEEKFGITV"
     gene            complement(1802..1900)
                     /locus_tag="PWY43_002282"
     CDS             complement(1802..1900)
                     /locus_tag="PWY43_002282"
                     /inference="COORDINATES: protein motif:HMM:NF028261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HDL2137117.1"
                     /translation="MSSAAIITMVVAMTIIWGGLLFAIKRLPKEEQ"
     gene            complement(1900..3408)
                     /locus_tag="PWY43_002283"
     CDS             complement(1900..3408)
                     /locus_tag="PWY43_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL2137118.1"
                     /translation="MSNQSSNAPQRETFSGRGAFIMAAIGSAVGLGNIWRFPYTTYEN
                     GGGAFILPYIIALLTAGLPLLFLDYSIGHKYRGGAPLSFRRFSSKFETFGWWQVLINV
                     IIGIYYAVVLGWAASYTYFSFSGAWGDKPVDFFIGEFLKMGDITQGVSFEFVGMVTGP
                     LIAVWLIAIAVLAMGIKKGISAASNLLMPLLIVMFLVLVGYSLTLPGAAKGLDALFTP
                     NWEKLADPSVWIAAYGQIFFSLSICFGIMITYASYLKKETDLTSSGLVVGFANSGFEL
                     LAGIGVFAALGFMATAAGQEVNEVAKGGIGLAFFAFPTIINQAPMGALVGVLFFGSLT
                     FAALTSFISVIEVIISAVQDKLNSGRVKATLLVGIPMMVVSVLLFGTTTGLPMLDVMD
                     KFVNQFGIVAVAFVSLIAIVTSEKLSTLEKHLNEVSSFKVGFIWRLCIVITSGVLAFM
                     LFSEIAKVYREGYEGYPSWFVNTFGWGMAIGLVVVAYLLSKMAWKNEPKGDE"
BASE COUNT         1095 a          856 c          724 g         1005 t
ORIGIN      
        1 gcctgtttaa ccgcagccga ttcactactt ggtttacgcc gtttaccgat atttttggta
       61 tctttgtggc ggacttttac ttttggtttt gccgcttctt ttttcgcctc ttttttcttc
      121 ttaatgcgtg ctttttcttt cttgctggtc acatttagct cgccatcttt cggggctttg
      181 gtgcgaggct ctaagccctc gataatgcga ggttttagta cttcttcggt gtaacgttta
      241 attttgccga gtaatttata atcgtgagct tccaccaatg aaattgccga accttttttg
      301 ccagcacgag cagtacgccc aatacggtgc aagtaagtat ccgcactata aggtaaatcg
      361 tagttcagca cgaaatcaat atcgtctata tcaatgcctc gagccgccac atcggttgcc
      421 actaacacat tcactacgcc ttcttttaaa cggttaatag cttggttacg ttgggtctga
      481 gccatttcac cttcgagata agtagaacga attccccgct tacgcaaggt ttcgctgagt
      541 tctcgcacat cttctcggcg gcggacaaac acaatcgctc tttccaccga aaattcgctg
      601 ataatacgag ctaataattt ggttttatgc tctacattat cggcgtggta ataccattgc
      661 tgaatttttt tacgctcacg gcgacttggt tcggcatcga ctttaatcgg atcatttaat
      721 aatcgctcgg caaaatcctc taataactcg ccctcgaggg tcgctgaaaa caaccaagta
      781 tgcttacgcc aacgggtttc tgccgcaatt ttctccgcat cttgcccgaa gcccatttgc
      841 aacattctat ctgcttcatc aaaaatcagc acttccaccg ctcggcagtc aaaattttcc
      901 tctttaatgt attgcattaa acgacccggg gtagcaacca caatatcttg atttttatta
      961 aagacttcac cgtgattttg atacgccacc ccaccggtaa tagtcgcaat gctcaactga
     1021 gtaaatttag ccagtgcttg ggcttgctcc gccacctgca ttgccagctc tcgggttggg
     1081 gtaagaatta aaatgcgagg agcccctggt ttgcagcgag gataatctaa taaatgctga
     1141 attgccggca gtaaaaaagc ggcggttttg ccggtaccgg taggggctga accgagcaga
     1201 tcacgtcctt ctaacgcagc cggaatcgtg gcttgttgaa tagcggttgg gcggttatag
     1261 cctttgtgtg ctaaggcttt aagtaattga gggcttaaat caagctcctc aaacgtcatt
     1321 aattgggtca tctctgtttc tctacttgta aatttgcccc agtatatcac attaactgct
     1381 tgccgtgatg gtgaagaaat ttatcctaaa aggctgaaaa aattgttaca cttacacaga
     1441 gccgtttaac ggacaattcg ctcactttta ctttatagaa ggaacattat atgtttgttg
     1501 aaatttatgg acgtttatct tgcccatact gcgtgcgtgc aaaaacatta gccgagaaaa
     1561 tgaaacaaga attaagcgat tttgatttta aatttattga tatgatcgct gaaaatattt
     1621 caaaacaaga cctagagcct cgtgttggca agccggttgc aactgtgcca caaatttttg
     1681 tggataataa ccacgttggc ggttgcaccg atttccaagc ctttgtggaa gagaaatttg
     1741 ggattaccgt atagccaaaa gcccgctaac aagcgggctt ttttatacaa aaatttgcaa
     1801 attattgttc ttctttcggc agacgtttaa tcgcaaaaag taaaccgccc cagataatcg
     1861 tcattgccac caccatggta ataattgctg ctgaactcat tactcatctc cttttggttc
     1921 atttttccac gccatttttg atagcaaata agccaccacc actaagccga ttgccattcc
     1981 ccagccgaag gtgtttacaa accagcttgg gtagccttca taaccttcac gatacacttt
     2041 ggcgatttcg ctgaataaca taaatgccag tacgccggag gtaataacga tacataaacg
     2101 ccagataaag ccgactttga atgaagaaac ttcgttcagg tgtttttcaa gggtacttaa
     2161 tttctcgctg gtgacaatcg caatcaagga aacaaacgcc accgcaacaa taccgaattg
     2221 gtttacgaat ttatccatca catcaagcat tggtaaaccg gtggttgtac cgaatagcaa
     2281 gacagatacc accatcatcg gaatgccgac tagtagcgtg gctttcacac gacctgaatt
     2341 gagtttgtct tgcaccgctg aaataatcac ttcaattacc gagataaatg aggttaatgc
     2401 cgcaaaggtg agtgaaccaa agaacagcac gccaactaat gcccccattg gtgcttggtt
     2461 aataatggtt gggaaagcga agaatgccag gccgataccg ccttttgcta cctcgttcac
     2521 ttcttgccct gcagccgttg ccataaagcc gagtgccgca aatacgccaa tacccgctaa
     2581 taattcaaaa ccactgttcg caaaacccac cactaagcct gaactggtta aatcggtttc
     2641 ttttttcagg tatgaagcat aggtaatcat aatcccgaag cagatagaga gtgagaagaa
     2701 gatttgaccg taagcggcaa tccacacact tggatcagcc aatttttccc agtttggggt
     2761 aaataacgca tctaagcctt tcgcagcacc cggtagggtt aatgaatagc cgaccaacac
     2821 taagaacatc acgatcaaca gcggcattag caggtttgag gcagcagaaa tccctttttt
     2881 gatccccata gccaacacag caatagcaat cagccatacc gcaattaacg gccctgtaac
     2941 cataccgaca aattcaaagc tcacgccttg agtaatatcg cccattttta agaattcacc
     3001 gataaagaaa tccaccggtt tatcgcccca cgctccgctg aatgagaagt aggtgtaact
     3061 tgctgcccag ccgagtacca ccgcataata aatcccgatg atcacgttga tcagcacttg
     3121 ccaccaaccg aaggtttcaa atttcgagct gaaacggcgg aaagagagcg gtgcaccacc
     3181 acgatattta tgcccgatag aataatctaa gaacagcagc ggtaaacctg cggtcaaaag
     3241 ggcaatgata tacggcagaa taaaagcccc accgccgttt tcataagtag tataagggaa
     3301 acgccagatg ttccctaagc cgacagcgga accaatagcc gccataataa atgcaccacg
     3361 tccggagaac gtttctcttt gcggtgcgtt tgatgattga ttagacacgg taaatacctc
     3421 aaactgataa agttttatat aaaaaagaat tgagttaaat gtaaattaat cacactttaa
     3481 gtgcggaata tgcccaattt caggttgaat gtaaagtaga aaaatataga aaaacagata
     3541 aaaaacgctc aattttcaat ataaaactcc aaataaaaac aaaaattcaa acaaaaaaac
     3601 taaaaacaag acatcttcag ttaaatataa ataaaaacct aaaagctacc agcataatta
     3661 tttatattaa aattaacaaa
//
LOCUS       DANKBV010000176         3616 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.176, whole genome shotgun sequence.
ACCESSION   DANKBV010000176 DANKBV010000000
VERSION     DANKBV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.176"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            50..3616
                     /locus_tag="PWY43_002284"
                     /pseudo
     CDS             50..3616
                     /locus_tag="PWY43_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484777.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1291 a          566 c          858 g          901 t
ORIGIN      
        1 aacctaaatg gagtttttag tcgttatcta ggacagcccc taatgttagt taggtcaaac
       61 agttaaattt accgatccag acagcaatat tgtgacaaca gttaaagata atgaaatcca
      121 atttagctta aattctacgt tatctgttgg taaagacggt aaagatggtc agccaggtga
      181 agccggtaca atcggcgtta aaggccaaga cggcaaagat ggtgtttcaa ttaaaggtga
      241 tgacagtaaa gacggcgtac caggcgaagc atcagttgtt gtaggtcgta atggtgccga
      301 tggtattgac ggtaaagttg gcgtaaatgg taaagacggt gcgtctgttg tattaaacgg
      361 caaagatggc tctatcggct taacaggccc gaaaggtgct gatggtcagc caggtgcaag
      421 cacaaatatt gctgttaaag atggcaaaca aggtgttgat ggcactggta aagatggctt
      481 accaggtgat aacggcgaaa ctcgtatcgt ttacaaaaat ggcgataaag aagagcaagt
      541 tgctaactta aatgatggct tgatctttac tggtaacaat gcagatacgt taaatcgtca
      601 taagctaaac acagtagtta atgttgtggg tgaaggtgtt gataaagcag catcagctgt
      661 atttaaatca gcagcaggta acatcaatgt taaagctgat ggtacagata agcttgaaat
      721 tcaacttaac cgtgagctta aagatttaac aaatgcgaaa tttgttgagt acaaacaagg
      781 tgatgatggt aaagaggttg ttgataatac taaaccatca acagagctta ctaataaagg
      841 tatcactatt acaccagcta atagtgaaaa tggcgcagat gctaaaccag tatcattaac
      901 agataaaggt ttagataacg gtaacaacca aattgttaac gtgaagagcg gtatcgatgg
      961 tgttaacggt caagatggtc aagatggtca accagttaaa tctgtaaatg atttaacaga
     1021 agagcagctt aaagaagttg gcacaaatgc agcgaatatc aatgatgtac gtaatgctac
     1081 tgatgatcta gtgaaaacgg gcttcaagat cactgctgat aattctaaac ttgcagatga
     1141 tgcaaaagaa gatactgtag agcttggtca aacagtagag tatcgtagtg aaagcggtaa
     1201 catcgttaca acggtacacg ataacaagat cgacttcgac ttagcgaata acctcaatgt
     1261 aggtggaaaa gacggtaaag acggcttaac atttaaatca gcggatggtg ctcaaggtgt
     1321 taatggtgaa gatggtgaaa acggcttacc gggtcaaaat ggcacaacac gtatcgttta
     1381 ccaaccgact gataaagacg gtaacccaca aggagatcct gaaactgttg caaccttaaa
     1441 cgatggctta atctttacag gtaataatga gttactaaac cgtcacaagc taaacacagt
     1501 agttaaaatt gtgggtgaag gtgttgataa ggcaacttct gaagcattta aatcagcagc
     1561 aggtaatatc aatgttgttg ctaatgagaa agatcagctt gaaattcagt taaaccgtga
     1621 cttaaaagat cttgctacag caaactttgt tgaatacaaa gaagacggtt ctataaatgc
     1681 agatgcaccg aaaacagttg taaacaacaa tggtttaacg gtaacaccga aagacggaaa
     1741 agatggtaaa gacggtaatg tagtatcgtt aaccaaagac ggtctaaata atggtggtaa
     1801 ccaaattgtt aacgtgaaga gcggtattga tggtattaat ggtcagcctg gtaaagatgg
     1861 taaagctgtt gaatctgtga atgatttaac agatgagcag ctagaaaaag taggtactaa
     1921 tgcagcgaac attaaagatg tacgtaatgc aacggatagc ttaatcaaca aaggctttaa
     1981 cattactgcg gataattctg aactggaaga tggtgctaaa gaagataatg tgaaattagg
     2041 ggaaaccgta aattacactt ctaaagataa aaacatcatc actaccgtaa gtaataatgc
     2101 gatcgacttt gccttaaaca acaatttaac cattggtggt ccgggtaaag acggtaaaga
     2161 tggtgttgat ggtaaggttg gtgttaaagg tgccgatggt acagctggtg tgaccttaaa
     2221 cggaaaagac ggctcaatcg gattaactgg tcctaaaggt caagacggta aagatggtgc
     2281 aagtgcaact atctctgtaa aagacggtgc taaaggcctt gacggtaacg acggtaaaga
     2341 tggcgaaagc aaaacccgta tcgtttatga gaaaccagac ggtacaactg aggaagttgc
     2401 aacactgaac gatggcttaa gattcgtagg tgatgacggt aaagttatca ataagaagct
     2461 aaatgaaacc ttacaaattt caggcggcga taccgattta gaggatttat ctgatgataa
     2521 tatcggcgtt gtgaataaag atggtaagtt aatggtgaaa ttagcaagaa acattgactt
     2581 aacagacgaa ggttcggtaa caatcggtga tacttatatt gacacgaatg ttgttggtgt
     2641 aggtgattcg gcattaaccg gtgatagctt aacagtaggt ggcgataatt cgattactgt
     2701 tgacggtaat aaaggtacaa tcggtggctt aaccaataag acatttgatc caaataactt
     2761 tactagtggt caagcagcaa cagaagacca gttaaaacag gtttatgatg tagcgaactc
     2821 tggttgggat attgcagcaa atggcaataa cagcaccaat gttggtccta aaggtaaagt
     2881 ttcatttaat aatgaagacg gtaatatcat cattactaaa gaaacgactg acaacaatgt
     2941 aacctttgca ttaaataacg atttaactat cggtggtccg ggtaaagatg gcacaatcgg
     3001 tatcaacggt aaagatggtt cgaatggcac tatgactttc gagcaaggta aaccgggtgt
     3061 tgatggtaaa gatggcgaaa cgaagactcg tatcgtttat gaaaccaaag acaaagacgg
     3121 caaccctgtt aaagaagaag tagcaacctt aaacgatggc ttaaaattcg ttggtgatga
     3181 tggtaaagtc attactaaga aactaaacga aacgttaagt attaccggtg gtgagaaaga
     3241 tgcaactaag ctctcagatg ctaataatat cggtgtagta aataacaacg gtactttaac
     3301 ggttaaatta gcgaaagaca ttgatttaac gaaagatggt tctgtgacta ttggtgatac
     3361 tacggtaaat aatgatggtt taactatcaa agatggccca agtgtaacta aagccggtat
     3421 caatgcaggc gataagaaaa tcactaacgt tcaagacggt gaaatttctg caaccagcaa
     3481 agatgcggta aacggtagcc agttatatca agtgcaagaa gttgctaacg caggttggaa
     3541 cttaacagca aatggtaaag acaaaggcaa cgttaaacca ggagcaacag ttgatctaaa
     3601 caacaaagac ggtaac
//
LOCUS       DANKBV010000177         3528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.177, whole genome shotgun sequence.
ACCESSION   DANKBV010000177 DANKBV010000000
VERSION     DANKBV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.177"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            75..1067
                     /locus_tag="PWY43_002285"
     CDS             75..1067
                     /locus_tag="PWY43_002285"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDL2137119.1"
                     /translation="MKKSFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSEVGNGM
                     QDNLSGIEKAVKVNVKCIPSSTYEVVHSLAKWKRHTLARFGFKEGEGLFVHMKALRPD
                     EDSLDRTHSVYVDQWDWEKVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSV
                     LPKQITFVHSEDLVQRYPNMTSKERENAICKECGAVFLIGIGGKLSDGKPHDGRAPDY
                     DDWTTESEQGYKGLNGDILVWNEELGTAFELSSMGIRVDETALRLQVSLTGDEDRLEM
                     DWHKDLLAGRLPLSIGGGIGQSRLVMFLLRKKHIGEVQSSVWPKAVFEEFENIL"
     gene            complement(1191..1610)
                     /gene="atpC"
                     /locus_tag="PWY43_002286"
     CDS             complement(1191..1610)
                     /gene="atpC"
                     /locus_tag="PWY43_002286"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL2137120.1"
                     /translation="MASQFELTVVSAERKIFEGTVTSVRVSGTDGELGVYAGHAPLLT
                     AIKPGMVKFTLADGKEEFIYVSGGFLEVQPTVVTVLADTAIRGEELDEQRILAAKRKV
                     EETLSKTSDADITAKLAREIAKLRVYELTKTKLANKR"
     gene            complement(1638..3011)
                     /gene="atpD"
                     /locus_tag="PWY43_002287"
     CDS             complement(1638..3011)
                     /gene="atpD"
                     /locus_tag="PWY43_002287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL2137121.1"
                     /translation="MATGKIVQIIGAVIDVEFPQDAVPKVYDALKVESGLTLEVQQQL
                     GGGVVRCIALGSSDGLKRGLKVENTNKAIEVPVGTKTLGRIMNVLGEPIDEAGPIGEE
                     ERWTIHRAAPSYEEQANSTELLETGIKVIDLVAPFAKGGKVGLFGGAGVGKTVNMMEL
                     IRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRVA
                     LTGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVL
                     QERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVVLSRNIASLGIYPAVDP
                     LDSTSRQLDPLVVGEEHYNVARGVQGTLQRYKELKDIIAILGMDELSEDDKRLVARAR
                     KIERFLSQPFHVAEVFNGVPGKFVPLKETIRGFKGILEGEYDHIPEQAFYMAGSIDEV
                     VERAKQM"
     gene            complement(3032..>3528)
                     /gene="atpG"
                     /locus_tag="PWY43_002288"
     CDS             complement(3032..>3528)
                     /gene="atpG"
                     /locus_tag="PWY43_002288"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDL2137122.1"
                     /translation="FFNPMGLEIKGHINGLGDTPAMEDLVGIVNGMVNAYREGEIDEV
                     YVVYNRFINTMSQKPTVQQLLPLPALENDSLEQTGSWDYLYEPNPQALLDSLLVRYLE
                     SQVYQAVVDNLASEQAARMVAMKAATDNAGNLINELQLVYNKARQASITNELNEIVAG
                     AAAI"
BASE COUNT          989 a          807 c          698 g         1034 t
ORIGIN      
        1 agcactcatc aattcttaaa tgcaatcaag cacgagaaat aaaaatcaac attctttaat
       61 taggaaagca aacaatgaaa aaatctttta ttttacagca acaagaaatc agtttcgtta
      121 aaaacacctt tacgcaatac cttattgata aattaggcat tatcgaagta caaggtccta
      181 ttttgagcga agtcggcaac ggtatgcaag acaacctttc cggtatcgaa aaagcggtaa
      241 aagtaaacgt aaaatgtatt ccaagctcta cttatgaagt ggttcattct cttgcaaaat
      301 ggaaacgcca caccttagct cgctttggat ttaaagaagg agaagggtta tttgttcata
      361 tgaaagctct tcgccctgat gaagattctt tagatcgcac acactcggta tatgttgacc
      421 aatgggactg ggaaaaagtc attcctgaag gtcgccgcaa cttcgatttc ttaaaagaaa
      481 ccgtaaacga aatctataaa gcaattcgtt taaccgaatt agcggtggag gctcgtttcg
      541 atattccatc tgtattgcct aaacaaatta ctttcgttca cagcgaagac ttagtgcaac
      601 gctatccgaa tatgaccagc aaagagcgtg aaaatgctat ctgtaaagag tgcggagcgg
      661 tgttcttaat cggtatcggc ggaaaattat cagacggtaa acctcacgat ggacgtgctc
      721 cggactacga tgactggaca accgaatccg aacaaggcta caaaggttta aacggtgata
      781 ttctcgtttg gaacgaagaa ctcggcacag cgtttgagct ttcctcaatg ggtattcgtg
      841 tggatgaaac tgccttacgc ctacaagtct ctttaaccgg cgacgaagac cgcctcgaaa
      901 tggattggca caaagattta ttagccggca gactcccgct ttctatcggc ggcggtatcg
      961 gtcaatctcg cttagtgatg ttcttactac gcaaaaaaca catcggtgag gtgcaatcca
     1021 gtgtctggcc aaaagcagta tttgaagagt ttgagaatat tctttaataa aacaaaaaag
     1081 cgaaccttag ggttcgcttt tgctttctac aagcggtctt attttgcaaa aaatttactg
     1141 tttttaaccg cttgtaaaac aaaaccccac tttcgagggg ttttttgata ttaacgttta
     1201 ttcgctaatt tggttttagt gagttcgtaa acacgcagtt tcgcaatttc tctcgctaac
     1261 ttcgcggtta tatccgcatc gctggttttt gaaagtgttt cttctacttt gcgttttgcg
     1321 gcaagaatac gctgttcgtc tagctcttcg ccacgaattg cggtatctgc caacactgtt
     1381 actacagtcg gttgtacttc aaggaaacca cctgaaacgt aaataaactc ttctttgccg
     1441 tcagctaaag tgaatttaac cattcccggt ttaattgcgg ttaatagcgg tgcatgccct
     1501 gcatacaccc ctaattcacc gtctgttcct gaaacacgca cgctggtaac tgtgccttca
     1561 aagattttac gttctgcact cactaccgtg agttcaaatt gagatgccat gttgttctcc
     1621 tttagtttag aacaaaatta catctgtttt gctctttcaa ccacttcgtc aattgaaccc
     1681 gccatataga acgcttgttc cggaatatgg tcgtattcgc cttctaaaat gcctttaaag
     1741 ccacgaattg tttcttttaa cggtacgaat ttgcccggta cgccgttgaa tacttccgca
     1801 acgtggaatg gttgtgaaag gaaacgttcg attttacgcg cacgagcaac aaggcgtttg
     1861 tcgtcttccg ataattcatc catacccaaa attgcaatga tgtcttttaa ctctttataa
     1921 cgttgtaatg taccttgtac accacgagca acgttatagt gttcctcacc aaccacgagt
     1981 ggatctaact gacgagaggt tgagtcaaga gggtcaaccg ccgggtaaat accgagtgaa
     2041 gcgatattac ggcttaatac caccgttgag tctaagtggg caaaggtagt tgccggagac
     2101 gggtcagtta agtcatccgc cggaacatat accgcttgca cagaggtaat agagccggtt
     2161 ttggttgagg taatacgctc ctgtaatacg cccatctctt ctgcaagagt cggctgataa
     2221 cctaccgcag acggcatacg acctaataac gcagatactt cggtaccagc taaagtataa
     2281 cggtagatgt tatccacgaa gaataatacg tcacgacctt catcacggaa tttttctgcc
     2341 atggttaagc cggttaatgc cacacgcaga cggttgcccg gtggttcgtt catttgaccg
     2401 taaaccagcg atactttgtc taatacgtta gagtctttca tttcgtggta gaagtcatta
     2461 ccctcacgag tacgctcacc tacaccggcg aataccgagt aaccgctatg ttcgatagcg
     2521 atgttacgga ttaactccat catatttacg gttttaccta cacccgcacc accgaataag
     2581 ccaactttac cacccttagc aaatggagca actaagtcga taactttgat accggtctct
     2641 aaaagctcgg tgctgttcgc ttgttcttcg tagcttggtg ctgcacggtg gattgtccaa
     2701 cgttcttctt caccgattgg gcctgcttca tcaattggct cacctaatac gttcataata
     2761 cgaccgagtg tttttgtacc aaccggtact tcaatcgctt tattcgtatt ttcaactttt
     2821 aagccacgtt ttaaaccatc agatgaacca agtgcgatac aacggaccac accgccacct
     2881 aattgttgtt gaacttcaag ggttaatcct gattcaactt ttaatgcatc ataaactttt
     2941 ggtactgcat cttgcggaaa ctcaacatcg attaccgcac cgatgatctg tacaattttt
     3001 cccgttgcca ttaccgttcc tctattcatt gttaaattgc tgcggcaccc gcgacaattt
     3061 catttaattc attcgtaata cttgcttgac gagctttgtt atacactaac tgtaactcat
     3121 taatcagatt acctgcgtta tcggttgctg ctttcattgc caccattcga gcagcctgtt
     3181 cagacgcaag attatctacc actgcctgat aaacttgaga ttctaaataa cgaaccagta
     3241 agctgtctaa taacgcttgt ggatttggtt catagagata atcccaagaa ccagtttgct
     3301 ctaatgagtc attttccagt gcaggcaaag gaagcaactg ttgtactgtc ggtttttgtg
     3361 acatcgtgtt tataaaacgg ttatatacca catacacttc atcaatttcg ccttcacggt
     3421 aggcatttac cataccatta acaataccga ctaaatcttc cattgccggt gtatcaccca
     3481 atccattgat atgaccttta atctctagcc ccattgggtt aaaaaagg
//
LOCUS       DANKBV010000178         3455 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.178, whole genome shotgun sequence.
ACCESSION   DANKBV010000178 DANKBV010000000
VERSION     DANKBV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.178"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..581)
                     /locus_tag="PWY43_002289"
     CDS             complement(<1..581)
                     /locus_tag="PWY43_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137123.1"
                     /translation="MKKYLMLCSLWFLSACFNESYDFRGKEFEEPVQAILVESDKIYF
                     IGSTIDAIFDKTLIDNTKASDVIYFLNKLLQSPYGRKISNHLITLAIYDENIQIQYSP
                     QFIAKNLTMSDIQALQKLGFQFSHSKEYLWKSYAGLGGKITQFKNRKEILEKYKFKQP
                     IIAKAKSYQKSIEFSFDDTISVIAAPITIPASI"
     gene            815..1105
                     /locus_tag="PWY43_002290"
     CDS             815..1105
                     /locus_tag="PWY43_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL2137124.1"
                     /translation="MALRPLHDKVILKREEVETKSAGGIVLTGSAATKSTRGKVIAVG
                     TGRILDNGSVQPLAVKVGDVVIFNEGYGVKTEKIDGEEVLILAEHDILAIVE"
     gene            1174..2814
                     /gene="groL"
                     /locus_tag="PWY43_002291"
     CDS             1174..2814
                     /gene="groL"
                     /locus_tag="PWY43_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608625.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL2137125.1"
                     /translation="MAAKDVKFGNDARVKMLKGVNILADAVKVTLGPKGRNVVLDRAY
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNE
                     GLKAVAAGMNPMDLKRGIDKAVAAVVEELKAISKPCETSKEIEQVGTISANSDSTVGQ
                     LIAQAMEKVGKEGVITVEDGTGLEDALDVVEGMQFDRGYLSPYFINKPEAGTVELENP
                     YILLVDKKVSNIRELLPVLEGVAKAGKPLLIIAEDIEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTAGTVISEEIGMELEKATLEELGQAKRVVISKDNTTIID
                     GIGDETQIKGRVAQIRQQIEDSTSDYDKEKLQERVAKLAGGVAVIKVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALVRAANKVADTLKGDNEEQNVGIKLALRAMEA
                     PLRQIVANAGEEASVVARNVKEGSGNYGYNAGTEQYGDMLEMGILDPTKVTRSALQFA
                     ASIAGLMITTECMITDLPKEEKLDPAAMGGMGGMGGMM"
     gene            complement(2939..3265)
                     /locus_tag="PWY43_002292"
     CDS             complement(2939..3265)
                     /locus_tag="PWY43_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDL2137126.1"
                     /translation="MLKRILGITLCTILVSSNAIASVKTSSLTVAKPTLSKNLPTECE
                     KMFNTANKLVSDAERQPGTHTQVLKMKNKLSLTKQQILKMDSELQQKSCEKGLTALNS
                     LKQKHQ"
BASE COUNT         1108 a          543 c          752 g         1052 t
ORIGIN      
        1 ataatagatg cgggaatcgt aattggtgct gcaatgaccg aaatggtgtc atcaaagcta
       61 aattctatgc ttttctgata acttttcgct ttagcgatta taggttgctt aaatttatac
      121 ttttctagta tttccttgcg atttttaaac tgtgtgattt taccaccaag acctgcatag
      181 cttttccata gatattcttt tgaatggcta aattgaaaac ctaacttttg tagagcttga
      241 atatcgctca tggtgagatt ttttgcaata aattgcggac tgtactgaat ttgaatattt
      301 tcatcataaa tggctaaagt aattaggtga ttactgatct tccttccata cggactttga
      361 agaagtttat tcaaaaaata aataacatct gaagcttttg tattatctat cagcgtttta
      421 tcaaatatcg catcaattgt ggaaccaatg aaataaattt tatctgattc aaccaaaatt
      481 gcttgtacag gttcttcaaa ttcttttcct ctaaaatcat aactttcgtt aaagcatgcg
      541 gagagaaacc atagtgagca gagcattaag tattttttca ttttattatc ctatcttgaa
      601 attaatagtt taaatggtat aagagaattg gggtttttct aaacagaaac agatttctta
      661 ggtatatagt tttatcaaaa atggtcaaat ttgtaaaaaa ttttacaaat ttgaccgctt
      721 gcacttgaaa ctctaaaatc tgcctttata ttgttaggcg ttacgcattt tgcgtgaagt
      781 tttatttatt catttttaag aaaaggaaca tattatggca cttcgtccat tacacgataa
      841 agttatttta aaacgtgaag aagttgaaac aaaatcggca ggcggtattg ttttaaccgg
      901 ttcggcagca acaaaatcaa cccgtggcaa agtaattgca gtaggaacag gtcgcatttt
      961 agacaatggt tctgttcagc ctttagcagt taaagtgggc gatgtagtga tttttaacga
     1021 aggctacggc gtaaaaaccg agaaaattga cggcgaagaa gtgctaattt tagcagagca
     1081 cgatattttg gcgattgtag aataatttcg ttctgcacaa gcggttaaaa aagcaaaatt
     1141 ttttacatat ttcaaataca aaggatttag aaaatggcag caaaagacgt gaaatttggt
     1201 aacgatgcac gcgtaaaaat gttaaaaggc gtgaatattt tagccgatgc ggtaaaagta
     1261 actttaggtc cgaaaggtcg taatgtggtg ttagatcgtg cttatggtgc accaacgatt
     1321 acaaaagacg gtgtgtcggt tgcccgtgaa atcgaattag aagataaatt tgaaaatatg
     1381 ggggctcaaa tggtgaaaga ggttgcctct aaagccaatg atgccgccgg tgacggtaca
     1441 actaccgcaa ctgtattagc tcaagcgatt gtaaacgagg gcttaaaagc agtggctgcg
     1501 ggtatgaacc caatggattt aaaacgtggt atcgataaag cggttgcagc agtggttgaa
     1561 gagttaaaag cgatttcaaa accgtgcgaa acctcaaaag aaattgagca agtagggacg
     1621 atttctgcaa actcagattc aacagtcggt caattaattg ctcaagcaat ggaaaaagtg
     1681 ggcaaagagg gcgtgattac tgttgaagac ggtacaggtt tagaagatgc attagacgtg
     1741 gttgagggta tgcaatttga ccgtggttac ttatcgccat actttatcaa taaaccggaa
     1801 gccggtacgg ttgagttaga aaacccatat attcttttag tggataaaaa agtgtctaac
     1861 attcgtgaat tattaccggt gttagaaggt gttgcaaaag caggtaaacc gttattaatt
     1921 attgccgaag atattgaagg cgaggcgtta gctaccttag tggtgaacac tatgcgtggt
     1981 atcgtgaaag tggcggcagt gaaagcacct ggctttggtg atcgccgtaa agcaatgtta
     2041 caagatattg cgatcttaac tgcaggtact gtgatttctg aagagatcgg tatggagctt
     2101 gaaaaagcaa cccttgaaga gcttggtcaa gcaaaacgtg tggttatcag caaagataac
     2161 accacaatta ttgacggtat tggtgatgaa actcaaatca aaggtcgtgt agcacaaatc
     2221 cgccaacaaa tcgaagattc aacttcagat tacgacaaag aaaaactaca agagcgtgta
     2281 gcaaaattag ccggtggtgt tgcggtaatt aaagtaggtg cggcaactga agtggctatg
     2341 aaagagaaaa aagaccgtgt tgatgatgct cttcacgcaa cccgtgctgc ggtggaagaa
     2401 ggtattgtac cggggggcgg agttgcttta gtgcgtgcag ccaacaaagt ggctgataca
     2461 ctaaaaggcg ataacgaaga gcaaaatgta ggtatcaaac ttgccttgcg tgcaatggag
     2521 gctccattac gccaaatcgt tgccaatgca ggtgaagaag cctcagttgt agcccgcaac
     2581 gtgaaagaag gttcaggcaa ctacggctat aacgcaggta cagagcagta cggcgatatg
     2641 cttgaaatgg gaattttaga cccaactaaa gtgactcgtt cagcacttca attcgcggct
     2701 tctattgcag gtttaatgat cactacagaa tgtatgatta ccgatctgcc aaaagaagag
     2761 aaattagatc ctgccgcaat gggcggtatg gggggaatgg gcggaatgat gtaattgccc
     2821 attgagtgaa caaaagctac tgaaattaat tcagtagctt ttttgttggt aacaagtggt
     2881 taaatttatg ctattttttg caaataaaaa accaagcaag taagcttggt tttagcgttt
     2941 attgatgttt ttgtttaagg ctgtttagtg ctgttaaacc tttctcacaa cttttttgct
     3001 gtaattcaga atccattttt aggatttgtt gctttgttaa agaaagttta tttttcattt
     3061 ttagcacttg agtatgagta ccaggctgtc tttctgcatc agagaccaat ttatttgccg
     3121 tgttaaacat tttctcacat tcagttggca aatttttact taaagtaggt tttgcaactg
     3181 taagtgatga ggttttaaca cttgctatag cattactgga aaccaatatg gtacaaagtg
     3241 ttattcctaa aattcttttt agcatgcgtt ctccacacat taagtgttta aaagtaaaaa
     3301 tctaatatgt tatttaataa agcttgctga tgttatcaga actttaattt aaagtcatct
     3361 aaaactaaga tctttttgcc aagaataaat tttcaagatc gttagtataa aaaatagtaa
     3421 ataaattgtt acttatttgt taagtataac cgtgt
//
LOCUS       DANKBV010000179         3393 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.179, whole genome shotgun sequence.
ACCESSION   DANKBV010000179 DANKBV010000000
VERSION     DANKBV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.179"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            15..521
                     /locus_tag="PWY43_002293"
     CDS             15..521
                     /locus_tag="PWY43_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137127.1"
                     /translation="MEAKYNMIIELAQQQGLTHIQLTQKHCLHLAASVGKLCSGVVNN
                     AKQKIKTATGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELYGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            525..884
                     /locus_tag="PWY43_002294"
     CDS             525..884
                     /locus_tag="PWY43_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137128.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKIQKNG
                     MLEVIEYRISQIKELLK"
     gene            881..1360
                     /locus_tag="PWY43_002295"
     CDS             881..1360
                     /locus_tag="PWY43_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137129.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKALNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYNFEQIEEDPEQVKPKPSKPKRK
                     PFTPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNALAEMVKRTQEANNPKHA"
     gene            1494..1706
                     /locus_tag="PWY43_002296"
     CDS             1494..1706
                     /locus_tag="PWY43_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137130.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFLLVEEKIIDVLENNGFETVAT
                     ILAGSDGWCNAINAYLAEQNKHRNEF"
     gene            1719..2642
                     /locus_tag="PWY43_002297"
     CDS             1719..2642
                     /locus_tag="PWY43_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL2137131.1"
                     /translation="MTNQNQQVAQKQDKFPIKTLFESPNVKQKLQELLNKNAATFTTS
                     VLQIVNSNSMLRNAEPMSVFNAACMAATLNLALQNGLGFAYIVPYQNRKEKKTEAQFQ
                     LGYKGLIQLAQRSGQFKRLVAVPVYEKQLLAEDPINGYEFDWKQKPAKDEKPIGYYAY
                     FKLINEFTAEIYMTTQEVHDHANRYSQTYRKGYGVWHDNFEAMALKTVMKLLLSKQAP
                     LSVEMQQAVLADQAVVKDVENAEFAYPDNEIQEAEFTELKVSDEVFEKCKQTIINKES
                     TLQDMCDNGYEFNKEQYAELEKLENEQQSNV"
     gene            2635..3297
                     /locus_tag="PWY43_002298"
     CDS             2635..3297
                     /locus_tag="PWY43_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation protein TolB precursor"
                     /protein_id="HDL2137132.1"
                     /translation="MYKFKPRCSMLHQLMSEPRTKADREAGLISDTAKAAVREVAKFD
                     LFGYQSFDGNKYTEKGNQLEEQAIKLSGFTRGLVLKKNTERRENDWISGECDIYVPSR
                     KLIIDTKCSWDIGTHPFFADEAIAKAEKAGYDIQMQGYMWLWDCEEAQIDFVLFPTPI
                     DLIGTYESTEKLIDLVEQIPQQKRITTVTVKRDEAIIAKIKDRVKAASKYYDKLIQEA
                     MN"
BASE COUNT         1218 a          730 c          677 g          768 t
ORIGIN      
        1 aaaaggaacg aaaaatggaa gctaaataca acatgattat tgagctggca caacaacaag
       61 gcttaaccca tattcaactt actcaaaaac actgtcttca tcttgctgca tcagtgggga
      121 aactatgttc tggagtcgtt aataatgcga aacagaagat taaaacggct acgggagaaa
      181 tttttacatt actcaccatc attaaccatc aaatccagtt cgatgaagac ttagagcttc
      241 acacgtggaa agctagtgga acaaccccac ccgagcaaag agcgatacgt atgttacatc
      301 atacctcgca atttgcttgg tttgctcctt ggttagtgga cagcaatgaa aaagaacaaa
      361 accaaaatac aatcaggatt aatttattta acaaatgctc tgggattctg attgaactat
      421 atggcattct taaagaatac gagctaactc ttgatgagtg cttagatgaa ttcttgaaaa
      481 ataagcattg tttgaatgaa tttagtattc acaaggagta aaaaatgacg acgtttagta
      541 tttataccag tgtatggcac gacagctcca cgaaaggctt tcgccacaac attaagcata
      601 aacgccacga ttgctggaga gcggatatcc gcataaagca aaaaatcaat gatgtgtggg
      661 tgcagatttc aagaatacgc aaacgcttca agtgccctga acaggcaaaa tcctttgtag
      721 aacaatggaa acaaattgat tcccctgcat ttatgcaaca ccaaatcaac cgccaagcat
      781 taagagaaga attatcaaac cttgagcaac gtaaaatcca aaaaaacgga atgcttgagg
      841 tgattgagta tcgtattagc caaatcaagg agttactgaa atgaaacgca ataaagtaga
      901 aagctatact atccgcttat taaaaaataa tcatacaaaa cgctacttcg gagaagttat
      961 attaaacggc ataaaagaaa tcagcacggc agagatgatg tctgaaatta acgctattaa
     1021 ggcattaaat aagcgtattg aagctctgaa taaaaccaaa gccgttaatt tgcctaatta
     1081 tcctgaaatc aggggtcatc agcctattat gtacaattac aattttgaac aaattgagga
     1141 agatcctgaa caggttaagc ctaaaccatc aaaaccaaaa cgcaaacctt tcacacccta
     1201 cgggctaaac ggctactttg tggatagcaa cggcaatatt cgcctgcacc tagacagacg
     1261 agcaagtgcc gaaacaatta cccttgaaac tgatttcttc aacgctcttg ccgaaatggt
     1321 aaaacgtaca caagaggcaa acaatcctaa acatgcttag acaatggatt acatacggct
     1381 tatacgccct gctttttatg gttgccatca ccctcctaat gcaatgaaga gcttatccct
     1441 gaaagcacct gcaagcctga aacttgcgaa tttaaagtaa cggagaaagt gtgatgatag
     1501 gatacgaaga ctacacagat gcttcacttc aaccagaaga agaccctagc gaagaggctt
     1561 tcctcttagt ggaggagaaa attatagatg tgctagaaaa caacggcttt gaaaccgttg
     1621 ctactatcct agccggttct gatggctggt gtaacgcaat taatgcctac ctggccgaac
     1681 agaacaaaca cagaaatgaa ttttagagga ataagataat gacaaaccaa aatcaacaag
     1741 tagcacaaaa gcaagataaa ttcccgatta aaaccctatt tgaaagcccg aatgtaaagc
     1801 aaaaactaca agagctttta aacaaaaacg cagccacgtt tacaacaagc gtgttgcaga
     1861 ttgtgaatag taactcaatg ttacgcaatg ccgaaccgat gagcgtattc aacgccgctt
     1921 gtatggcagc aacgcttaat ttggcattac aaaacggctt aggttttgcc tacattgtgc
     1981 cgtaccaaaa ccgcaaagag aaaaaaaccg aagctcaatt ccaacttggc tacaaaggtt
     2041 taattcaact ggctcaacgt agcgggcaat ttaaacgcct tgttgccgtg ccggtgtatg
     2101 aaaaacaact gcttgccgaa gaccccatca acggttacga gttcgattgg aaacaaaaac
     2161 cggctaaaga tgaaaaaccg attggctatt acgcttattt caaactgata aatgaattta
     2221 ccgccgaaat ctatatgacc acgcaagaag tccacgacca cgccaaccgc tacagccaaa
     2281 cctaccgcaa aggctacggc gtatggcacg acaactttga ggcaatggct ctcaaaactg
     2341 tgatgaagct cttgctttcc aaacaagctc cattatcagt ggaaatgcaa caagcggtct
     2401 tggcagatca agcggtcgta aaagatgttg aaaatgcaga atttgcctac cctgacaacg
     2461 aaatccaaga ggcggaattt accgaactca aggtcagcga tgaggtgttt gagaaatgca
     2521 aacaaaccat catcaacaaa gaaagcacct tgcaagatat gtgcgataac ggctacgagt
     2581 tcaacaaaga gcaatatgcg gagttggaga agttagaaaa tgaacagcaa tcaaatgtat
     2641 aaattcaaac cccgttgctc aatgctccac caactaatga gcgaacctcg caccaaagcc
     2701 gaccgtgaag ccggactgat ttccgacacc gcaaaagcag cggtgcgtga agtggctaaa
     2761 tttgacctat tcggttatca atcctttgac ggcaacaaat acactgaaaa aggcaatcag
     2821 ctagaagaac aagccattaa gttaagcggt tttactcgtg gcctggttct aaaaaagaac
     2881 accgaacgcc gagaaaacga ttggatttca ggcgagtgtg atatttatgt accaagccga
     2941 aagctgatta tcgatacaaa atgcagttgg gacatcggca cgcacccgtt ttttgcagac
     3001 gaagccatcg ccaaagccga aaaagccgga tatgacattc aaatgcaagg ctatatgtgg
     3061 ctgtgggatt gcgaagaggc tcaaatcgat tttgtgttat tccctactcc aatcgacctt
     3121 atcggtactt acgaaagcac agaaaagctg attgaccttg tggaacaaat cccccaacaa
     3181 aaacgcatta ccaccgtgac ggttaagcgt gatgaagcca tcattgccaa aatcaaagac
     3241 agagtgaaag cagccagtaa gtattacgac aagcttatac aagaagctat gaattaattc
     3301 aatcgccccg aaaggggctt tttaataggt gacaaatatg acaacccaaa agaaatacga
     3361 actcctccaa aacgacacaa tcaaccacaa cgg
//
LOCUS       DANKBV010000180         3379 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.180, whole genome shotgun sequence.
ACCESSION   DANKBV010000180 DANKBV010000000
VERSION     DANKBV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.180"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1904)
                     /gene="rsxC"
                     /locus_tag="PWY43_002299"
     CDS             complement(<1..1904)
                     /gene="rsxC"
                     /locus_tag="PWY43_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDL2137133.1"
                     /translation="MSNPNLVIERIRQNKETGKLWDFPGGIHPMENKSQSNSKPIRTL
                     NLPKFFYVPLVQHSGWAGELLVKVGDVVLKGQPLTKGDHFRQLPVHSPTSGRVIGIEP
                     YVSAHASGLPEITVIIETDGKDKWVERQPIEDFLSLTSDQIIQKVYQYGIAGLGGAVF
                     PTATKLSLADKRCKLLVINGAECEPYITCDDRLMQDFTADLIEGIRILRYVLRPEEVV
                     IAIEDNKPHAIQALETALKGSNDMFVRAIPTKYPSGASDQLIQVLTGLEIPAGKRTIE
                     MGIVMHNVGTAFAVKRAIIDDEPLIERVVTLTGDKIRNKGNVWARLGTPIQHLLEQVD
                     YQADKRFPVFLGGPMMGFILPALQAPITKTANCLIAPDHFEYAPPEPERSCIRCSSCS
                     DACPVGLLPQQLYWFARAEDHDKSKEYHLDACIECGVCAYVCPSYIPLIQYFRQEKAK
                     IEEIEEKARKAEEAKIRFEAREARLQKERDARTARISQAAEKRREEVANAGGVDPVKA
                     ALERLKAKKAVETVQEIPAEVQVKPNGEPDNSDLMAQRRARRLAKQAEQAVEKSENVT
                     NALIGANNDLPLQSMNETASDKSVGANNYSPEAESEDPRKAAVAAALARAKAKKQAQQ
                     AVEKSENITNA"
     gene            complement(1891..2508)
                     /gene="rsxB"
                     /locus_tag="PWY43_002300"
     CDS             complement(1891..2508)
                     /gene="rsxB"
                     /locus_tag="PWY43_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDL2137134.1"
                     /translation="MLDLPIIAYILIAIAVIALIFGAVLGYSSIKLKVESDPIVDQID
                     ALLPQSQCGQCGYPGCKPYAEAIANGDVITKCVPGGQPLVVNLAELLGVEVPPMEGMV
                     EPEMKVAFVIEDLCIGCTKCIQACPVDAIIGTNKAMHTIIPDLCTGCELCVAPCPTNC
                     IEMRPIKPTTQSWNWKFDQDLIIPIVNTTELQRKLVTGGGKNEQS"
     gene            complement(2508..3089)
                     /gene="rsxA"
                     /locus_tag="PWY43_002301"
     CDS             complement(2508..3089)
                     /gene="rsxA"
                     /locus_tag="PWY43_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDL2137135.1"
                     /translation="MVDYILLVISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGLA
                     TTFVLTVASLSAYLMETYLLEPLNAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRL
                     LGIYLPLITTNCAVLGVALLNVNLANNLVESVLYGFGAAAGFSLVLVLFAALRERLAA
                     ADVPRPFQGASIALITAGLMSLAFMGFTGLVKL"
BASE COUNT          924 a          817 c          709 g          929 t
ORIGIN      
        1 gcattggtaa tattttctga tttttcgacc gcttgctgtg cctgtttttt ggctttggct
       61 cgggcaaggg cagccgcaac ggcggctttg cgtgggtctt cgctttctgc ttcgggcgaa
      121 tagttattcg cccctacaga tttatccgat gcagtttcgt tcattgattg taggggcaaa
      181 tcattatttg ccccaatcaa ggcatttgta acattttccg atttttcgac cgcttgttct
      241 gcctgtttgg ctaaacgtct ggctctgcgt tgtgccatta aatcgctgtt atcaggctcg
      301 ccgttaggtt ttacctgtac ttctgccggt atttcttgga ctgtttcaac cgcttttttc
      361 gctttcaggc gttcaagagc cgcttttaca ggatcaacac cacctgcatt tgccacttct
      421 tcacggcgtt tttccgccgc ttggctaatg cgagcagttc tggcatctcg ttctttttgt
      481 aagcgagctt ctctcgcctc aaagcggatt ttagcctctt ccgctttgcg ggctttttcc
      541 tcaatttcct cgatttttgc tttttcttgg cggaaatatt ggattaacgg aatgtagctt
      601 gggcaaacgt aagcacaaac gccacactca atgcaggcat ctaaatggta ttctttcgat
      661 ttatcgtgat cttctgcacg ggcaaaccaa taaagttgct gtggcaataa ccccaccggg
      721 caagcatcgg aacagcttga acagcggata caactacgct ccggttcagg tggtgcatat
      781 tcaaaatgat caggggcaat caagcagttc gcagttttgg taatcggggc ttgcaatgca
      841 ggcaaaataa agcccatcat cggtccgcct aaaaacaccg gaaagcgttt atcggcttga
      901 taatctacct gctccaacaa gtgctgaatt ggcgtgccta atcttgccca cacgttcccc
      961 ttgttgcgaa ttttatcgcc tgtcagcgtc actacccgtt caatcaaagg ttcatcatca
     1021 ataattgccc gtttaaccgc aaacgccgtg ccgacattgt gcatcacaat gcccatttca
     1081 atagtgcgtt tgccagccgg aatctcaagt ccggtcaaca cttgaatcag ctgatcagaa
     1141 gctccggagg gatatttagt cgggatcgcc cgcacaaaca tatcgttcga gccttttaaa
     1201 gcggtttcta aagcttgaat agcgtgcggt ttgttgtctt caatcgcaat caccacttct
     1261 tccgggcgaa gcacataacg cagaatgcga ataccttcga taagatccgc agtgaaatct
     1321 tgcattaagc gatcgtcaca ggtgatgtaa ggctcacatt ctgccccgtt aatcaccagt
     1381 aatttacagc gtttatccgc caaacttaat tttgttgcgg ttgggaatac cgcaccgcct
     1441 aaaccggcaa tgccatattg gtaaactttt tggataattt gatcgcttgt taggcttaaa
     1501 aaatcctcaa tcggctggcg ttccacccat ttatctttac catcggtttc aataatcacc
     1561 gttatttcgg gcaaacctga ggcgtgagcg gaaacataag gctcaatccc aatcacccgc
     1621 ccggaggttg ggctgtgtac gggcagttga cggaagtgat cgcctttcgt gagcggctgc
     1681 cctttcaata ccacatcgcc gactttgact aataattccc ctgcccagcc agaatgttga
     1741 accagtggca catagaaaaa cttcggtaaa tttaaggtgc gaattggctt gctgtttgat
     1801 tgggatttat tttccatcgg gtgaataccg cccgggaaat cccacagttt acccgtttct
     1861 ttattttggc ggattcgctc aatcactaaa ttaggattgc tcatttttgc ctccgccggt
     1921 aactaatttt ctctgcaatt cagtggtatt cacaatcgga ataattaaat cttggtcaaa
     1981 tttccagttc caagattggg ttgtcggttt aattggtcgc atttcaatac agttggtcgg
     2041 gcaaggggcg acacaaagtt cacagccggt gcaaagatcc ggaataatgg tgtgcattgc
     2101 tttattcgtg ccgataatcg catcaaccgg gcaagcctga atgcacttag tacagccaat
     2161 gcagagatct tcaatcacaa aagccacttt catctcaggc tctaccatac cttccattgg
     2221 cggcacttct accccaagca attcggcaag attcaccact aaaggttgtc cacccggcac
     2281 acatttagtg atcacatcac cattagcaat cgcctcagca taaggcttac agcccgggta
     2341 gccacattga ccgcattggc tttgtggtaa aagggcatca atttgatcga caatcgggtc
     2401 ggattcaact ttcagtttaa tcgaggaata gcctaaaacc gccccgaaaa tcagtgcgat
     2461 cacagcaata gcgataagaa tataagcgat aattggcaaa tcaagcatta gagtttcacc
     2521 aatcctgtaa agcccataaa ggcaagcgac atcaagcctg cggtaatcag agcaattgat
     2581 gctccttgga acggacgagg cacatcagcg gcagccaaac gctcacgcag agccgcaaat
     2641 aaaactaaca ccagcgagaa accggcagcg gctccaaaac cataaagaac agattccacc
     2701 aagttattgg ctaaattcac gtttaataat gccaccccta atacggcaca gttggtggta
     2761 atcagcggta aatagatccc aagcaagcga taaagtgtcg ggctggtttt atgcacaatc
     2821 atttccgtta attgcacgat caccgcaatc actaaaataa acaccaaggt acgcaaaaat
     2881 tgagcgttta acggctcaag caaataggtt tccatcaaat aagcagagag cgaggcaacc
     2941 gtcagcacaa aggttgtcgc caaccccata ccaatagcag tttccacctt tttcgacact
     3001 cccataaacg gacaaagccc tagaaattta actaagacaa agttattaat aagggcggtg
     3061 ctgatgacca acagaatata atcgaccata aattccacaa aattaaatat acaagcggtc
     3121 aaaaacgcat tttattttgc aaaaaacaga gaaaatctga ccgtttgtta aagcatctca
     3181 ttaaaaataa acgttatatt atctcttgtt ttcggctcat tcacaataag aaatcagagg
     3241 tacttctagt aaacatacca tatcaaatat gatcttgtta cctttgtttc tatctctggt
     3301 agaatggaga atgtgaggac taagctatac agcatgaaag gtgaaaggag cttgattttc
     3361 taagttaatc ctaaaaagt
//
LOCUS       DANKBV010000181         3345 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.181, whole genome shotgun sequence.
ACCESSION   DANKBV010000181 DANKBV010000000
VERSION     DANKBV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.181"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(93..263)
                     /locus_tag="PWY43_002302"
     CDS             complement(93..263)
                     /locus_tag="PWY43_002302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137136.1"
                     /translation="MTEQLNTQSKSGEEDQEKSQDRYAVGWPSRRISDCVQHFRSRKI
                     YPFASNNDVKQG"
     gene            complement(289..474)
                     /locus_tag="PWY43_002303"
     CDS             complement(289..474)
                     /locus_tag="PWY43_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137137.1"
                     /translation="MYNLDDAIQECREKFGEDKPYFSEAGTLTFAGCKALSRLRDEKL
                     GIKAPPNFPFPRVEDLS"
     gene            complement(491..566)
                     /locus_tag="PWY43_002304"
     tRNA            complement(491..566)
                     /locus_tag="PWY43_002304"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(531..533),aa:Trp,seq:cca)
     gene            983..1177
                     /locus_tag="PWY43_002305"
     CDS             983..1177
                     /locus_tag="PWY43_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137138.1"
                     /translation="MKLSTNELKALSDERRGVRAKSYKLSLETIALIEQLSKQLDMPQ
                     NQLIKLAVEKLQEQTNLTTN"
     gene            complement(1145..1585)
                     /locus_tag="PWY43_002306"
     CDS             complement(1145..1585)
                     /locus_tag="PWY43_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137139.1"
                     /translation="MLIDIKGVLNLWGSFVRFTDCKGYPTMQAFMRESPQEREKQRYL
                     PRLDDDTLAKVDRQIRKLDPAGKAKAYLANLAKPNDKREELESLESDELQFAILYLRY
                     VAGLENKPIWQKLSLSKGAFENELRLAERFVEGVLIGSEIRLFL"
     gene            complement(1575..1934)
                     /locus_tag="PWY43_002307"
     CDS             complement(1575..1934)
                     /locus_tag="PWY43_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL2137140.1"
                     /translation="MVELILPYPPSVNDYWRWVSKTRRVVCKRGKDYQWATFLATRNK
                     PKFGGQVEIECDVYFPDNRDRDLDNLGKCILDSLVYSKVIVDDSRKYVKKLTFEDKGN
                     QKGGAVIVRIKERLNVN"
     gene            complement(1927..2955)
                     /locus_tag="PWY43_002308"
     CDS             complement(1927..2955)
                     /locus_tag="PWY43_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL2137141.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDISPAAFGERTLLQPAPTDL
                     ATVKSGKVANFLANSTACKELAEFAKSAKVRSTLNQRDKYSNDLPYMRFVKSIQTCQC
                     RDGKYCHPERVIANIENGAVQLCWHHDKMRMDGEIPTEKLEALADKNWERFIAQQIRL
                     TLRKTQATVIEFADLVLFASIKGLLNELDDNALQHFLGYPALNNSGVNKESNIGFDDV
                     PNARANLKQWAEKLKLKVEPEPLAAFMAKPKLQRFEWRKWLQYVKAQPCMCCGKQADD
                     PHHIIGYGGAMGSKQHDLFVIPLCRIHHDELHRNVGKFEQDYGSQLELLIKFLHRALG
                     LGVLEIDG"
     gene            complement(3082..>3345)
                     /locus_tag="PWY43_002309"
     CDS             complement(3082..>3345)
                     /locus_tag="PWY43_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL2137142.1"
                     /translation="VVMLLPADKTTEWYTIIQQHANEVIDIIGYHDEKGTWRTGRLQF
                     INPVTGKPAQGNNKGSMIVVFDPFIEGLVTRQMPLNKIKEWGK"
BASE COUNT          972 a          788 c          564 g         1021 t
ORIGIN      
        1 ttggttaaaa attctgctag aatactgttc cccaacaaat aaactagcag agggtaaaaa
       61 tgattgaatt tactctgtcc catcagaagc atttaacctt gcttcacgtc attattactg
      121 gcaaatggat aaattttcct acttcggaag tgctgaacgc aatctgatat acgtcgagat
      181 ggccagccca cggcatagcg atcttgagat ttttcttgat cttcttcacc gctttttgac
      241 tgagtattta gttgttccgt catagcaatc cctcattgat ttttgtagct aagatagatc
      301 ttcaaccctt gggaaaggga aatttggcgg agccttaatt cctaactttt catctcttaa
      361 tcgagagagg gctttgcaac cagcaaaagt aagtgttcca gcttcagaaa aataaggctt
      421 atcttcacca aatttttctc tgcattcttg aattgcatca tcaaggttat acataacatt
      481 tactcacgtt tggcaagggt ggctggactc aaaccaacaa ccaacgattt tggagaccgt
      541 tgctctacct attgagctac acccttaaaa taaaaagccc caagcatttc tgctcagggc
      601 tgtaaaattc tttttaagtt caccacctat gcaatgaacc gcaacttacc acaaataata
      661 cactttatac ttaagctata caacactttt taatcaaaca aatacaaaaa agcccataat
      721 ttacaaaact atgggctttc taatgaaaat tcacttgttt tattcggtgt tccgaactat
      781 aataatctca ctttcaacgg ttcgggttga aagtgagtgt gaggcttaat cctcacgctt
      841 gaagaaggaa caaacgatgt ttaagtacat tgtcctagtt atcttcttgt tagttatcag
      901 ctccccagcc tactaacttg aaataactcg ctgggggcga aagctcccag ctcttcaaac
      961 agggtcattt taggaattta gaatgaaatt gtcaaccaat gaattaaaag cactatctga
     1021 tgaaagaaga ggggtgagag cgaaatctta caagctaagc ctcgaaacta ttgctcttat
     1081 tgaacaatta tcgaagcagt tagatatgcc tcaaaaccaa ctgattaagt tagccgttga
     1141 gaaactacaa gaacagacga atctcactac caattaatac tccctccaca aatcgctcgg
     1201 caagtcgcaa ttcattttca aaggccccct tgctgagcga caacttctgc caaataggct
     1261 tattttctag ccctgcaaca taccgcagat acaaaatagc aaattgcagt tcatcactct
     1321 cgagtgattc taactcctca cgcttatcgt tcggtttagc taaattggca aggtatgctt
     1381 tggctttacc ggctgggtct agttttctaa tctgccgatc aaccttggct aaggtatcat
     1441 catctaatcg aggtaaatag cgttgttttt ctctctcttg cggagattca cgcataaacg
     1501 cctgcattgt tggatagcct ttgcaatccg taaacctaac gaaactaccc cataagttta
     1561 atacaccttt aatatcaatt aacattcaaa cgctccttaa ttctcacaat gaccgcaccg
     1621 cctttttgat tgcctttgtc ctcaaatgtg agctttttca catattttcg ggagtcatca
     1681 acaatcactt tgctatatac caacgaatct aaaatacact tacctaagtt atccaaatcc
     1741 cgatctcggt tatcggggaa gtaaacatca cactcaattt caacctgtcc gccaaatttc
     1801 ggcttatttc gagtagcaag aaatgttgcc cattggtaat ctttacctcg tttacacacc
     1861 actcgtcttg ttttgcttac ccaccgccaa tagtcattta cgcttggcgg atagggcagt
     1921 attaattcaa ccatctattt ctaatacccc caaacctaac gctcgatgta aaaacttaat
     1981 caacaactct aattgcgagc cgtaatcttg ctcaaactta cccacattac gatgtaattc
     2041 atcatggtgg attcggcaga gtggtattac aaataaatcg tgctgtttac tccccatcgc
     2101 tccgccatag ccgataatat gatgtggatc gtctgcttgt ttaccgcaac acatacaagg
     2161 ctgagctttt acgtactgca accacttccg ccattcaaac cgctgtaatt tcggttttgc
     2221 cataaatgcc gccaacggct ccggctcgac tttcagtttt aacttttcag cccattgttt
     2281 tagattggct cgagcattcg gcacatcatc aaagccgata ttactttctt tattcacacc
     2341 gctattattc aaagccggat aacctaaaaa gtgctgtaag gcattatcat ctaactcatt
     2401 taacaaacct ttgatactcg caaacaacac taagtccgca aattcaatga cggtagcttg
     2461 tgtcttgcgt aaggttaagc ggatttgttg ggcgataaat ctttcccagt ttttatctgc
     2521 caatgcttct aatttttcag ttgggatttc accgtccatt cgcattttgt cgtgatgcca
     2581 acataactgt actgcaccat tctcaatatt tgcgattact ctttctgggt gacagtattt
     2641 accatctcgg cattggcacg tctgaattga ctttacaaac cgcatatacg gcaaatcatt
     2701 actatatttg tcacgctgat ttaacgttga acgaactttt gccgatttag caaactctgc
     2761 caactcttta caagcggtcg aatttgccaa aaaatttgca accttgcctg atttaaccgt
     2821 cgctaaatcg gtcggtgcag gttgcaacaa agtccgctca ccgaaagcag caggagatat
     2881 atctttaggc actttgtaaa acacaatacc cacttcggtt tgaaagtaag gggtaagtag
     2941 caaaccttcc gccattattg cccccatttc tcggtttcat cataggcttt cacgaatagc
     3001 ataaatacgc caatacagat taaaaaaatc agtacgccaa tcactgcacc aataccaata
     3061 aatagttccc aataatccat tttatttacc ccattccttg attttattaa gtggcatttg
     3121 acgagtaacc aatccctcta tgaacggatc aaacacaaca atcatcgagc ctttattatt
     3181 gccctgagcc ggtttacctg ttactgggtt aataaattgt agtcgtcctg tacgccaagt
     3241 gcctttttca tcgtgatagc ctatgatgtc tataacctca ttagcgtgtt gctggataat
     3301 ggtgtaccat tctgtagtct tatccgctgg taacagcatt accac
//
LOCUS       DANKBV010000182         3251 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.182, whole genome shotgun sequence.
ACCESSION   DANKBV010000182 DANKBV010000000
VERSION     DANKBV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.182"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..745
                     /gene="aroC"
                     /locus_tag="PWY43_002310"
     CDS             <1..745
                     /gene="aroC"
                     /locus_tag="PWY43_002310"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2137143.1"
                     /translation="KYGIRDYRGGGRSSARETAMRVAAGAIAKKYLREQFGIEVRGYL
                     SQIGNIKIDPKTVENIEQIDWHAVNSNPFFCPDPVAIEGFDALIRELKKDGDSVGAKL
                     TVIAERVPVGLGEPVFDRLDADLAHALMSINAVKAVEIGDGFAVVEQRGSQHRDEMTP
                     QGFLSNHAGGILGGISSGQPIIAHIALKPTSSITIPGRSVNKNNEAVEVITKGRHDPC
                     VGIRAVPIAEAMMAIVLLDHLLRFKAQCR"
     gene            764..1591
                     /gene="mepA"
                     /locus_tag="PWY43_002311"
     CDS             764..1591
                     /gene="mepA"
                     /locus_tag="PWY43_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDL2137144.1"
                     /translation="MKLLKTLLVSSLAFTATALNATQWEKIKTPVQGKAQSIGGYSNG
                     CIIGAQPLALKGEGYQVIRSARNRYYGHPQLLDYLTYLGKKTKAAGVPTILIGDMGMP
                     AGGRFASGHASHQTGLDADIWFKFGPMDDETARNPAGLATIMVNRSTERVDDSLWNQH
                     HTNLVKLAATDPRVDRIFVNPAIKVKLCQTAGNDRAWLRKIRPWYGHDSHFHVRLTCP
                     ADAASCENQAPVPAGDGCGEELYSWFEPKEASGTPKAKVLPTAPVQCQMILSEQGLN"
     gene            1664..>3251
                     /locus_tag="PWY43_002312"
     CDS             1664..>3251
                     /locus_tag="PWY43_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2137145.1"
                     /translation="MRLFVAEKPSLARAIADVLPKPHTKGDGFIQCGEHDTVTWCIGH
                     LLEQAEPDVYDERFKMWRMEHLPIVPEQWKLIPKKETLKQFKVVEKLIKKADVLVNAG
                     DPDREGQLLVDEVFGYLNLSAERKSQIQRCLVSDLNPNVVSRAIEKLQKNSDFIPLAT
                     SALARARADWLYGINMTRAYTLQGRWAGYKGVLSVGRVQTPVLGLIVRRDLEIEHFVP
                     KDYFEVLAQIVVPETQERFKAQWQPSKACEDYQDEDGRVLSRPLAENVVKRITDQPAT
                     VTEYQNKIEKETAPLPYSLSVLQMEAAGRYGLSAQAVLDICQKLYETHKLITYPRSDN
                     RYLPTEHFNDRFKVMAAIAHHLPEYQQKPEVIDATIRNRCWNDKEVEAHHAIIPTARQ
                     GTVHLTENEKRVYQLIARQYLMQFCPDAEYRKSKIGLEIAGGSFVAQARNLVIAGWRA
                     LLGKEDSLDDAEPLLPIVKKGQILHCEKGEIVSKKTQPPRHFTDRSLLSAMTGIARFV
                     QDKELKKILRETDGLGTEATR"
BASE COUNT          920 a          750 c          791 g          790 t
ORIGIN      
        1 aaaatacggc attcgagatt accgtggcgg tgggcgttct tctgctcggg aaaccgcaat
       61 gcgggttgcc gccggggcga ttgccaaaaa atatttgcgt gagcaatttg gcattgaagt
      121 gcgaggctac ctctcacaaa tcggcaatat caaaattgac ccgaaaaccg ttgaaaatat
      181 tgaacaaatt gattggcacg cagtcaatag taatcctttc ttctgccccg atcccgttgc
      241 gattgaaggc tttgatgcct taattcgtga gctgaaaaaa gatggcgatt cggtgggggc
      301 aaaattaacc gtgattgcag agcgtgtacc tgtagggtta ggcgaacctg tgtttgaccg
      361 cctagatgcc gaccttgccc acgccttaat gtcgatcaac gccgtgaaag cggtggaaat
      421 tggcgatggt tttgcagttg tggaacaacg tggtagccag caccgagatg agatgacgcc
      481 gcaaggcttt ctctctaatc acgccggcgg aattttaggt ggtattagct ccggtcagcc
      541 gattatcgct catattgcgt taaagccgac ctcaagcatt actattcccg gcagaagcgt
      601 gaacaaaaac aacgaagccg ttgaagtgat caccaaaggc agacacgacc cctgtgtagg
      661 tattcgtgct gtgccgattg cagaagcgat gatggcgatt gtactactcg accatctgtt
      721 aagatttaag gctcaatgcc gttaaacaca taaaattgga aaaatgaaat tattaaaaac
      781 acttttagtc tcaagccttg cttttacagc taccgcactg aatgcaacgc aatgggagaa
      841 aatcaaaact ccggttcaag gaaaagcaca atctatcggc ggttatagta acggctgtat
      901 tattggggca cagccccttg cattaaaagg cgaaggctac caagtgatcc gctcggctcg
      961 taaccgctat tacggacacc cacaactgct cgattatctc acttacttag gcaaaaaaac
     1021 caaagctgcc ggtgtgccaa ccattttaat cggcgatatg ggaatgcctg ccggtgggcg
     1081 ttttgcctcc ggtcacgcct cccatcaaac cgggctggat gctgatattt ggtttaagtt
     1141 cggaccgatg gacgatgaaa ccgcccgcaa tccagcaggg cttgccacca ttatggtaaa
     1201 ccgtagtacc gagcgggttg atgattcact ctggaatcaa caccatacta atttagtaaa
     1261 acttgctgca accgatcctc gagtggatcg catttttgtc aatcctgcca ttaaagtgaa
     1321 actctgccaa accgcaggca atgatcgtgc ttggttacgc aaaattcgcc cgtggtacgg
     1381 acacgactcc catttccacg tccgtttaac ctgcccggct gatgccgcaa gttgtgaaaa
     1441 tcaagcccct gtgcctgccg gtgacggctg tggtgaagag ctttactcgt ggtttgagcc
     1501 gaaagaagcc tccggcacac caaaagccaa agtgctaccc acagcgcctg ttcaatgtca
     1561 gatgatttta tctgaacaag gcttgaatta attgcaaaat aacaaccttt tttgaccgct
     1621 tgtcagccca caagcggttc attttcttaa ataatttacg attatgcgtt tatttgttgc
     1681 cgaaaaaccc agccttgccc gagcgattgc cgatgtgtta ccaaaacccc ataccaaagg
     1741 agatggcttt atccaatgcg gcgaacacga cactgttact tggtgtatcg gtcatttatt
     1801 ggaacaggct gaacccgatg tctatgatga gcgtttcaaa atgtggcgaa tggaacattt
     1861 gccgattgtg cctgaacaat ggaaattgat tcccaaaaaa gagacgctca agcagtttaa
     1921 agtggtggaa aagctgatta aaaaagccga tgttttagtc aatgccggag acccggatcg
     1981 agaagggcag ctgttggtgg acgaagtttt tggctatttg aatttatctg ctgaacgcaa
     2041 aagccaaatt cagcgttgtt tggtaagcga tctcaacccg aatgtggtga gccgagccat
     2101 tgaaaaattg caaaaaaaca gcgattttat accgcttgca acctctgctc tcgcccgagc
     2161 cagagcggat tggctgtatg gtatcaatat gacccgagcc tataccctac aggggcgttg
     2221 ggcaggctat aaaggcgtgt tatcagtcgg acgtgtgcaa acgccggtat tagggctgat
     2281 tgttcgccga gatctggaga tcgagcattt cgtgccaaag gattattttg aggtgttggc
     2341 acagattgtc gtgccggaaa cccaagagcg atttaaggcg cagtggcaac cgagtaaagc
     2401 ctgtgaagat tatcaagatg aagatgggcg ggtgctgtct cgtccattgg cagaaaatgt
     2461 ggtaaaacgg attaccgacc agccggcaac cgtcaccgaa tatcaaaata aaattgaaaa
     2521 agaaactgca ccgctgcctt attctctttc ggtattacaa atggaagccg ccggcagata
     2581 tgggctatcc gcccaagcgg tgttggatat ttgccaaaag ctgtatgaaa cccataaact
     2641 aattacctac cctcgttctg ataatcggta tttgccgacc gaacatttca acgacagatt
     2701 taaagtgatg gcggcgattg ctcatcattt acctgaatat caacaaaaac cggaagtgat
     2761 tgatgccact attcgcaatc gttgctggaa tgataaagag gtggaagccc accacgccat
     2821 tattcccact gcacggcaag ggacggtaca tttaaccgaa aatgaaaaaa gagtttatca
     2881 actcattgcc cgccaatatc taatgcaatt ctgccctgat gcggaatatc gcaaaagtaa
     2941 aatcgggctt gaaattgcag gtggtagttt tgtcgctcaa gcacgcaacc ttgtaattgc
     3001 cggctggcga gcgttactgg gtaaagaaga tagccttgac gatgcagagc cactactacc
     3061 gattgtcaaa aaagggcaaa tcctccattg tgaaaaaggg gaaattgtca gcaaaaaaac
     3121 acaaccgccc cgccacttta ctgatcgcag tttgctttca gcaatgaccg ggatcgcccg
     3181 ttttgtacaa gataaagaat tgaaaaaaat cttgcgtgaa accgatggtt taggcacgga
     3241 ggcaacccga g
//
LOCUS       DANKBV010000183         3249 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.183, whole genome shotgun sequence.
ACCESSION   DANKBV010000183 DANKBV010000000
VERSION     DANKBV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.183"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..422)
                     /gene="orn"
                     /locus_tag="PWY43_002313"
     CDS             complement(<1..422)
                     /gene="orn"
                     /locus_tag="PWY43_002313"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDL2137146.1"
                     /translation="MAQNEQNLIWIDLEMTGLDPEKERIIEIATIVTDKDLNILAEGP
                     VLAVHQTDDLLEKMSDWCVKTHTANGLIERVKASKLTERAAELQTLDFLKRWVPKGAS
                     PICGNSIAQDKRFLYKYMPDLADYFHYRHLDVSTLKELA"
     gene            complement(432..911)
                     /gene="coaD"
                     /locus_tag="PWY43_002314"
     CDS             complement(432..911)
                     /gene="coaD"
                     /locus_tag="PWY43_002314"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL2137147.1"
                     /translation="MSYNVIYAGTFDPITNGHLDIICKASHLFGKVIVAVAQNPSKQP
                     LFSLEERVELVKQSCTQWQNIEVIGFSGLLADFAKQQNAKALIRGIRGADDIDYEIQL
                     AQLNYKLSGDLETIFFPPSIEWRYLSSTMVREIYRHNGDISQFVPQAVKNALKIAKV"
     gene            complement(913..2193)
                     /gene="waaA"
                     /locus_tag="PWY43_002315"
     CDS             complement(913..2193)
                     /gene="waaA"
                     /locus_tag="PWY43_002315"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDL2137148.1"
                     /translation="MLRLLYICLSYLLQPVVLLLMWYKGRKQPAYRKRLWERYGIYDE
                     SEKPKAKGVVIHAASVGEVIAATPLIKAVSKQYPELPLIVTTVTPTGSGRVQAAFGNS
                     VSHFYLPYDLPDAIERFLNFIDPKLMIVIETELWPNLIRKVNIRKIPFVIANARLSPR
                     SAKRYGWVKGSIKDMLNQISLIMAQDEVSRDRYLALGYAPAKMVNTGNLKFDLEITPQ
                     LHQSVIKTKASLNLTERPIWIAGSTHEGEEKLILDAHKLLLEHYPNLVLILVPRHPER
                     FGLVELLIQKSDLNYVKRTENKTLSQNTQVMLGDTMGEMMLLYGLSDIAFVGGSLVKH
                     GGHNPLEPIAFNIPVVSGLYTYNFPEIFEKLREVKGVIEIESSVEALTESIQLLLKQP
                     QIGQEIAKSGLSVLQENQGALKRHLDLLASYLEK"
     gene            complement(2473..>3249)
                     /locus_tag="PWY43_002316"
     CDS             complement(2473..>3249)
                     /locus_tag="PWY43_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HDL2137149.1"
                     /translation="RWEYKMEEGKIDEVGQIVPKYEPEIEVKNVAPTLGNGEATDKVF
                     LYTESQRPDEQMTAFEDEHGTYFMNSKDLRAVQHVERLTQMGVHSLKIEGRTKSFYYC
                     ARTAQVYRKAIDDAAAGKPFDESLMDTLESLAHRGYTEGFLRRHTHDEYQNYEYGYSI
                     SERQQFVGEFTGKRNAEGLAEVAVKNKFLVGDSVEMMTPKGNIVFKIERMLNRKNENV
                     DAALGDGHFVFLDVPADIELDFALLMRNLVNTNTRNPHQA"
BASE COUNT          918 a          753 c          590 g          988 t
ORIGIN      
        1 gccagctctt ttaaagtact cacatctaag tggcgataat ggaaataatc ggctaaatca
       61 ggcatatatt tatacaaaaa acgtttatct tgagcaatgc tgttgccaca aatcggagaa
      121 gcgccttttg gcacccagcg ttttaagaag tctaaagttt gcaactctgc tgcccgttca
      181 gtcaatttgc tcgctttcac tcgttcaatc agcccgttag cggtatgggt tttgacgcac
      241 caatcactca ttttctctaa taaatcatcg gtttggtgta ccgcaagtac cggtccttcc
      301 gctaaaatat ttaaatcctt atcggtcaca atggtggcga tttcaataat gcgttctttt
      361 tccggatcta accctgtcat ttcaagatcg atccaaatta aattttgttc gttttgagcc
      421 attttatttc cttacacttt tgcaattttt agggcatttt tgaccgcttg cggcacaaat
      481 tggctgatgt caccattgtg gcgatagatt tcccttacca tcgttgaaga aaggtaacgc
      541 cattctattg aaggggggaa aaaaatagtt tctaaatcgc ccgataattt gtaatttaac
      601 tgagcgagtt ggatttcata gtcaatatca tctgccccac gaataccacg gattaacgct
      661 ttggcattct gctgtttggc aaagtctgct aacaatccac taaagccaat cacctcaata
      721 ttttgccact gggtacagct ttgtttaaca agctccactc gttcttcaag actaaaaagc
      781 ggctgcttgc ttgggttttg agcaacggca acaatcactt ttccaaataa gtgagaggct
      841 ttgcaaatta tgtccaaatg cccattggta atgggatcaa aggtgcctgc ataaattacg
      901 ttataactca ttttatttct ctaaatagga ggctaataaa tccaaatggc gttttaatgc
      961 cccttgattt tcctgtaaca cgcttaagcc ggattttgca atttcttgtc cgatttgggg
     1021 ctgcttaagc aataattgaa tgctctcggt caaagcctcg actgaacttt caatttcaat
     1081 cacgcctttc acctcacgca atttctcaaa gatttcaggg aaattatagg tgtaaagccc
     1141 agaaaccacc ggaatattaa aagcgattgg ttcaagtgga ttatgcccac cgtgtttgac
     1201 caaactgcct cctacaaaag caatgtcaga caacccatac agtaacatca tttcccccat
     1261 tgtatcacct aacattactt gagtattttg gcttaacgtt ttattctcag tacgttttac
     1321 ataatttaaa tctgattttt gaatcagtag ctcaactaaa ccaaaacgct ccggatggcg
     1381 aggcactaaa atcaacacta aattcgggta atgctctaat aagagtttat gagcatctaa
     1441 aatcagcttc tcttcccctt cgtgagtact gccggcaatc caaatcggtc tctctgttaa
     1501 attcagacta gctttagttt taatcaccga ttgatgaagc tgtggcgtaa tttctaaatc
     1561 aaatttcagg ttaccggtat taaccatttt tgccggtgca taaccgagtg ctaaatagcg
     1621 atctcggctt acctcatctt gagccataat cagactaatt tggttgagca tatccttaat
     1681 gctacctttt acccagccat aacgcttcgc agaacgtggc gataagcggg cattggcaat
     1741 cacaaatgga attttacgaa tatttacttt acgaattaaa ttcggccaaa gttcggtttc
     1801 aattacaatc atcaatttcg gatcgataaa attcaaaaac cgctcgatag catcaggtaa
     1861 gtcataaggc aaataaaaat gtgaaacact attaccaaaa gcagcttgca cacgcccaga
     1921 gcctgttggc gttaccgttg tgactatcaa cggaagttca ggatattgct tgctgactgc
     1981 cttaatcaaa ggcgttgcag caatcacctc accaaccgat gccgcatgga ttaccacccc
     2041 ttttgctttc ggtttttccg attcgtcata aatcccataa cgctcccata aacgtttccg
     2101 ataagccggt tgtttacgcc ctttgtacca cattaatagc agaaccacag gctgtaatag
     2161 ataacttaaa caaatataga gtaaacgtag catttatttt ccttatacca taaccctcgc
     2221 ctcttgtggg agagggggaa tattgcaaac tgttacaaaa aaacgaccgc ttgccctctc
     2281 tctgataaaa ttgctgaaca caattttatc ggtctctctc ccacaagggg agagagtcaa
     2341 atacgccaat aaatcgttcc tctcttgttc gagagggggg aacaagcggt tatttttacc
     2401 aaaaattttg caatctatat cctcaaagac aaaaccacgg aataacctta atccgtggtt
     2461 tatctcgcat cattacgctt gatgtggatt gcgggtattc gtattcacta agttacgcat
     2521 taaaagtgca aaatccaact caatatctgc cggcacatct aaaaagacaa aatgcccgtc
     2581 accgagtgct gcgtccacat tttcattttt gcggttaagc atacgctcga ttttaaatac
     2641 aatgttgcct ttaggggtca tcatttccac tgaatcgccc actaagaatt tgtttttcac
     2701 tgccacttcc gccaagcctt ctgcattgcg tttgccggta aattcgccca caaattgttg
     2761 gcgttctgaa atcgaatagc catattcata gttttggtat tcatcgtgag tatgacgacg
     2821 taagaagccc tctgtataac cacggtgtgc aaggctttca agagtatcca ttaaactttc
     2881 gtcaaacggt ttaccggcag cggcatcatc aatcgcctta cgatacacct gagccgttct
     2941 cgcacagtaa tagaacgatt tcgtacgccc ctcaattttc agtgaatgca cccccatttg
     3001 agttaaacgc tcaacgtgtt gtactgcacg cagatcttta gagttcataa aataggtgcc
     3061 gtgttcatct tcaaaggcgg tcatttgctc atcagggcgt tgtgattctg tataaaggaa
     3121 cactttatct gttgcttcgc cattacctag agtaggagcc acatttttga cttcaatttc
     3181 cggctcatac ttaggcacaa tttgccctac ctcatcaatc ttaccttctt ccattttata
     3241 ttcccaacg
//
LOCUS       DANKBV010000184         3228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.184, whole genome shotgun sequence.
ACCESSION   DANKBV010000184 DANKBV010000000
VERSION     DANKBV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.184"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            105..287
                     /locus_tag="PWY43_002317"
     CDS             105..287
                     /locus_tag="PWY43_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL2137150.1"
                     /translation="MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKE
                     IGTGLVNKIKKDLDLK"
     gene            326..745
                     /locus_tag="PWY43_002318"
     CDS             326..745
                     /locus_tag="PWY43_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL2137151.1"
                     /translation="MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGL
                     ATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMKVFLLNEMIKQGVRKAEMARRLD
                     VHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH"
     gene            complement(812..1117)
                     /locus_tag="PWY43_002319"
     CDS             complement(812..1117)
                     /locus_tag="PWY43_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDL2137152.1"
                     /translation="MRKPAHPGQVLLDGFIEPNNIKIKELADHLGFSRETLSRVLHGK
                     TPMTANLALSLEEAGISTAKLWLNLQTKYDLWELKQQRLNPVMPFHFGGNYGETAHA"
     gene            complement(1126..1401)
                     /locus_tag="PWY43_002320"
     CDS             complement(1126..1401)
                     /locus_tag="PWY43_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2137153.1"
                     /translation="MIKSFKHKGLKQYFEKGITKGIQLNHQRKIDGILDLIDSAESID
                     EFMPFYQCHELKGNRKGIYSMTVNGNWRITFEFVNGDAYILNYEDYH"
     gene            1691..1921
                     /locus_tag="PWY43_002321"
     CDS             1691..1921
                     /locus_tag="PWY43_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137154.1"
                     /translation="MNAQTTLNNHKDYILCGRKEKRTSDFINVFEVFENEATQEFVIE
                     RAMFRNGKLIDWNQSDKMNAEQAQQLWQAYIH"
     gene            complement(2400..2723)
                     /locus_tag="PWY43_002322"
     CDS             complement(2400..2723)
                     /locus_tag="PWY43_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005715000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137155.1"
                     /translation="MWEVLIPSIFSIIKEHFDNIVMRITTWFLSFIICWLYIPIDIQD
                     HLLANALPGLPTYTLLYIFYLVAATAFWQMFILCLDVFALIINKIIKSPAMDQQTNRV
                     DVDNK"
     gene            2916..3101
                     /locus_tag="PWY43_002323"
     CDS             2916..3101
                     /locus_tag="PWY43_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137156.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGVWQ
                     VESNGNQYCGGKYDTKY"
     gene            3085..>3228
                     /locus_tag="PWY43_002324"
     CDS             3085..>3228
                     /locus_tag="PWY43_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137157.1"
                     /translation="MTRNIETGVEIVAVGKLQHSTYPQYSRQNAIMLINRKIERFNVG
                     RDKP"
BASE COUNT         1118 a          617 c          599 g          894 t
ORIGIN      
        1 tcgtggcggt tttcttttct gctacaaaaa aacacaacaa aaatgttgca ataccaacaa
       61 aaatgttgta ttatttcctt gctgttttga taaggaggta tcttatgaaa tacagcgagt
      121 tcctgaggta cttacttgct caaggttgcg aaattgaaaa ccataggcga ggcagtcatc
      181 gaaaggtaac actaaacgga aaacaatcgg tttttcctta tcacgggagc aaagagattg
      241 gtacaggttt agtaaataag attaagaaag acttagatct taaataacaa agtccctcga
      301 aagaggggct tttctaggag tttatatgtt acgctaccca gttgaaatta cccctgatga
      361 taacggcaca taccttgtga cttgccctga tattcctgaa atggcaagcg taggtgagga
      421 cttagaagaa gccctattag aagcacaaga tggcttagcc acagcccttg aattctattt
      481 tgatgataga cgagaaatac caatgccaag cccaattaaa gagggacaac atacggttaa
      541 tcttaccgtg ctgcaatcaa tgaaagtatt tctcttaaat gaaatgatta aacaaggtgt
      601 acgtaaagca gaaatggcaa gacgattaga tgtacactta ccacagatag atcgcctatt
      661 agattttaat cattccacaa aagtagaatt tgttgagaaa gcctatggta agctaaatca
      721 acgctttact attctccctc actaataaaa taaccgccta cgggcggttt tcttttccta
      781 agaacaataa aaccctccga agagggcatt attaagcgtg agcagtttct ccataatttc
      841 caccaaaatg aaaaggcata acaggattta accgctgctg ctttaactcc cataaatcat
      901 atttggtttg taagttgagc cataacttag ccgtactaat tcctgcttct tctaaactta
      961 acgccaaatt tgcagtcatt ggtgttttgc cgtgtaatac tcttgataaa gtttcacgag
     1021 aaaacccaag atgatctgct aattctttaa ttttgatgtt atttggttca ataaacccat
     1081 ctaataaaac ttgacctgga tgtgctggtt tacgcataac tccccctaat gataatcttc
     1141 ataattcaaa atataagcat cgccatttac aaattcaaat gtaatccgcc agttgccatt
     1201 aaccgtcata gaataaatgc cttttcgatt tccttttaac tcgtggcatt ggtaaaaagg
     1261 cataaattca tcaatacttt ctgctgaatc aatcaaatct aaaataccat cgattttacg
     1321 ctgatgatta agctgaatac cttttgttat ccctttttca aaatattgtt ttaagccttt
     1381 atgcttgaaa ctcttaatca taatgatacc cgccaaacaa atgtgatata aatatatcac
     1441 aaaaaataag taaggcaaga tatttcgagt gtattcctgt gtaattatcc tttctctcta
     1501 tgcattctgt ttaaaaaaca agcaatcaaa caacctttct aaaaataaat tctctttaaa
     1561 atcaataaat tactaaacaa aagctaaact ttatttagtt tacactaaat aaacacttta
     1621 ctaaatgttt aatatttact aaactacacc catcaaaaca aacaacgcca cagacaaagg
     1681 ggaaaccaaa atgaacgcac aaacaacgct aaacaaccac aaagactaca ttttatgcgg
     1741 acgcaaagaa aaacgcacca gtgatttcat caatgtgttt gaggtctttg aaaatgaagc
     1801 cacgcaagag tttgtgattg aaagagcaat gttcagaaat ggcaagttaa tcgactggaa
     1861 ccaaagcgac aaaatgaacg ctgagcaagc tcaacaactt tggcaagcct acattcacta
     1921 agaattttta tcaaagccct ttactgaggg cttgaataaa ggttctagac ctagcccaca
     1981 tagcaggcaa tagccgagag ataagcagag actgtgggat gttctttaac aatttagtgc
     2041 ttgtgcggac gatattaaca acctcgagca gttgtaaagt aatgctttat cactcggcaa
     2101 ggttggttag accctgacat acaaatttga acgacttgcc aaacagaggt ggcaaggttt
     2161 aggcaacaca ctcgcaaggt gtggagagct gaaaaggcaa cactggtatc agtcctaagc
     2221 aatcacgctt atctcaaatt tgagtaagta cggatacaag cagcacccga cggaatggca
     2281 aaagccggac gggcgacggc gtgggcagac acgcattatt ccaaagcgta tttaagaaag
     2341 tatgttctgg aatatttaac tagaggtaaa ttatgaaggt aaaacaatgc ttattaattt
     2401 tatttattat caacgtcaac cctattggtt tgttgatcca tagctgggct tttaataatt
     2461 ttattaatta tcaatgcaaa gacatccaag caaaggataa acatttgcca aaaagctgta
     2521 gcagcgacca aataaaaaat atagagcaat gtataagtag gtagtcctgg tagggcatta
     2581 gccagtaaat gatcttgtat atctattggt atatatagcc aacagataat aaaggacaga
     2641 aaccaagttg taatacgcat aactatgtta tcaaagtgtt cttttattat agaaaagata
     2701 gagggtatca atacttccca cattgtaaag ttcctgattt ttagtgatga gaggagaggt
     2761 ctttaattat attcctgcga gtggtgagag acaagcaggg cttgctgtcc gtggcaagta
     2821 taaaatcacg gacactgttt actacaatct agacgtaagt cgctccattt gcccaccgta
     2881 aaacgtgggc tttttttacc cgaaaggaca atcctatgaa caaaatgaaa caacttgccc
     2941 catttgcctt gctaattttg ctattaggca ttgtcgggcg aatggattat gacgaccaca
     3001 tacaaatgca acgttacaaa tgcgaacgaa atcaaggcgt ttggcaagtg gaaagcaacg
     3061 gcaatcaata ctgcggaggc aaatatgaca cgaaatattg aaacaggcgt agagattgtg
     3121 gcggtcggca aattgcagca ttcaacctat ccacaataca gccgccaaaa cgcaattatg
     3181 ttgattaacc gcaaaattga gcggttcaac gttggcagag ataaaccg
//
LOCUS       DANKBV010000185         3192 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.185, whole genome shotgun sequence.
ACCESSION   DANKBV010000185 DANKBV010000000
VERSION     DANKBV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.185"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            126..761
                     /locus_tag="PWY43_002325"
     CDS             126..761
                     /locus_tag="PWY43_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDL2137158.1"
                     /translation="MFSELIPALPWSFLFIQDLIGTIVFAISGAIAARQHKMDIFGMF
                     ILAFVTGVGGGTLRDMMIGSTPVFWMKQPIYVLMITLAVVITAIFRNQISRKSWQTGL
                     LVFDAIGLGVFTVIGVQKGLDFGLHPLICVALGGVTGCFGGLIRDILRNEVPIILQKE
                     VYVTASLIGGVIFVAANSVGLESDWVYLATVFTVIIIRLLAIRFNLNLPRL"
     gene            807..1862
                     /gene="rsxD"
                     /locus_tag="PWY43_002326"
     CDS             807..1862
                     /gene="rsxD"
                     /locus_tag="PWY43_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDL2137159.1"
                     /translation="MFKMVSSPHTHSSNLTANFMLWVIAMILPAFGVQLYYFGYGVLI
                     QTLLAVSLAIVIEIGIAKLRKKTTAFYLADLSGILTALILAMSIPPYAPYWLVLIGVI
                     VALLLAKHSYGGLGQNLFNPAMVGYALLLVSFPVQMTAWIVPIELLSEPPTFRDAISL
                     IFYGVTSDGFSIHQLVNSVDGIAQATPLDSMKSSLAKSGFDAVMNTPIFNGLFATGWL
                     QLNLAFLFGGLVLIYKRIIHWQIPVAMLATFAFFSGFTELFSQQSHLHFVSHFFSGAM
                     MFGAFFIATDPVTASITPRGKLIFGGLVGLLVYLIRYYGNYPDAIAFSVLLANICVPL
                     IDHYTQPRLYGTKLDNR"
     gene            1862..2494
                     /gene="rsxG"
                     /locus_tag="PWY43_002327"
     CDS             1862..2494
                     /gene="rsxG"
                     /locus_tag="PWY43_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDL2137160.1"
                     /translation="MNTSKITLNYALILGFIALLCTAVSTGVYLLTKGKIDDVTAMQQ
                     RKLLEEVVPKSHFDNDVLATCKLVQLENAPYLNRIYVAKKSENLTAYLVQGTAPDGYS
                     GDIVLLMGIEPNGNILGVRTLEHKETPGLGDKIETRVSDWILSFTNQLFSLENEADWN
                     VKKDGGKFDQFTGATITPRAVVNHIRQSAKWVVTELAQNPTMFNQFKACK"
     gene            2503..3186
                     /locus_tag="PWY43_002328"
     CDS             2503..3186
                     /locus_tag="PWY43_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDL2137161.1"
                     /translation="MEREQQIPVTNIDVAPKQSSVWKTIFADGVWKNNGALVQLLGLC
                     PLLAVSNSVTNALGLGLATMFVLICTNITVSLFRKITPHDIRIPIYVMVIATVVTAVQ
                     LLMNAFAFPVYQSLGIFIPLIVTNCIVIGRAEAYASKNEVKFSAFDGFSMGLGMTFSL
                     VVLGALREIIGNGTLFSGLDLLLGDWAKALRIDVLHIDSGLLLAILPPGAFIGLGVIL
                     AVKNIIDKR"
BASE COUNT          787 a          644 c          790 g          971 t
ORIGIN      
        1 taaatctgta ggggcgaata accattcgcc caaaacggaa agcgaagacc cacgcaaagc
       61 cgccgttgcg gccgccatcg ccagagctaa agcgaaaaaa gccgctcagc aggagaaaag
      121 caacaatgtt tagcgagcta attccggcat tgccgtggtc gttcctgttt attcaggact
      181 taatcggcac tatcgtgttt gcgatttcgg gggcaattgc tgcccgccag cacaaaatgg
      241 acattttcgg tatgtttatt ttagcgtttg tgaccggggt cgggggcggt acgctaaggg
      301 atatgatgat tggctccacg ccggtgtttt ggatgaaaca accgatctat gtgctgatga
      361 ttacccttgc agtggtgatt accgctattt tccgcaacca aatttcccgc aaaagttggc
      421 aaaccggctt gctggtgttt gatgccatcg ggcttggcgt atttaccgtg attggcgtgc
      481 aaaaaggctt ggatttcggc ttgcacccac tgatctgtgt ggctctgggc ggtgtgacag
      541 gctgttttgg tggcttaatc cgtgatattt tacgcaacga agtgccgatc attttgcaaa
      601 aagaagtgta tgtgaccgcc agccttatcg gtggtgtgat ttttgtggct gcaaactcag
      661 ttgggctcga aagcgattgg gtttaccttg ccaccgtgtt caccgtaatt attatccgcc
      721 tgctggcaat acgctttaat ttgaatttgc cgagacttta ataagcggtc atttttttgc
      781 aattttttac taaaaaggtt ttatagatgt ttaaaatggt cagttccccc cacactcatt
      841 cgagcaatct cactgcgaat tttatgctgt gggtgattgc gatgatctta cctgcttttg
      901 gggttcagct ttattatttc ggctatggtg tgctgattca aacgttgctt gccgttagcc
      961 ttgcgattgt gattgaaatc gggattgcaa aattacgcaa aaaaacaacc gctttctatt
     1021 tggctgactt atcaggtatt ttaaccgcct tgattttggc gatgtcgatt ccgccttatg
     1081 ctccttattg gcttgtgttg attggcgtga ttgtggcgtt attattggca aaacacagtt
     1141 atggtggctt ggggcagaat ctatttaacc cggcaatggt tggctatgcg ttgttgttgg
     1201 tttctttccc ggtgcaaatg acggcgtgga tagtgccgat tgagttatta agtgaaccac
     1261 cgacatttcg tgatgcgatt tcgttgattt tttacggggt aactagcgat ggttttagta
     1321 ttcatcagct agttaatagt gttgatggca ttgcacaagc taccccgctt gatagtatga
     1381 aaagcagcct tgcaaaatca ggttttgacg cggtaatgaa tacgccgatt ttcaacgggc
     1441 tgtttgcgac cggttggcta caactgaacc ttgcgttctt attcggcggt ttggtgttga
     1501 tttataaacg gattatccat tggcaaattc cggtagcaat gttggcaact tttgcctttt
     1561 ttagtggatt caccgagctg tttagccaac aatcgcactt gcattttgtg agccatttct
     1621 ttagcggtgc gatgatgttc ggggcatttt ttattgcgac cgacccggta acagcctcca
     1681 ttaccccaag aggaaaactg attttcggtg gcttggtggg attattggtt tatctaatcc
     1741 gctactacgg aaattaccct gatgcgattg cattttcagt gttattggca aacatttgcg
     1801 taccgttaat cgaccattat acgcagccga gattatacgg cacaaaattg gataacagat
     1861 aatgaacacc tcaaaaatta ccttaaatta cgccttaatt ttaggcttta ttgccttgct
     1921 ttgcaccgct gtttcaaccg gtgtgtacct gcttaccaaa ggcaagattg atgatgtaac
     1981 cgcaatgcaa caacgcaaat tattggaaga ggttgtgccg aaaagccatt ttgataatga
     2041 tgtgttggcg acttgcaaac tggttcagct cgaaaatgcc ccttatttga accgtattta
     2101 tgttgcaaaa aaatcggaaa atctgaccgc ttacctagtt caaggaacgg ctccggacgg
     2161 ctattccggc gatattgtgc tattgatggg gattgagccg aacggcaaca ttttaggcgt
     2221 tcgcaccctt gagcataaag aaactccggg gctgggagat aaaattgaga cccgagtgtc
     2281 ggattggatc ttatctttta ccaatcaact ttttagttta gaaaacgaag cggattggaa
     2341 tgtaaaaaaa gatggcggca agttcgacca atttaccggt gcaactatta cacctcgtgc
     2401 ggtggtcaat catatccgcc aaagtgcgaa atgggtggtg acagagctgg ctcaaaaccc
     2461 gacaatgttt aatcaattta aagcgtgcaa atagggggag aaatggaaag agaacaacaa
     2521 atccctgtga cgaatattga cgttgccccg aaacaatcca gcgtttggaa aacgattttc
     2581 gctgatggtg tgtggaaaaa taatggtgct ttggtgcaat tattagggct ttgcccacta
     2641 ctggcggttt caaacagcgt aaccaatgca cttggactag gcttggcaac aatgtttgtg
     2701 ctaatttgca ccaatatcac ggtttccctg tttcggaaaa tcactccaca cgatattcgg
     2761 attccgattt atgtgatggt aattgcaacg gtggtaacgg cagtacagct tttaatgaat
     2821 gcttttgctt tccctgttta ccaatcgctc gggattttta ttccgctgat tgtaaccaac
     2881 tgtatcgtga ttggacgagc agaagcctat gcctcaaaaa atgaggtcaa attctcggct
     2941 tttgacggct tttcaatggg gctgggaatg acttttagcc ttgtggtgct aggcgcgttg
     3001 cgtgaaatta ttggcaacgg cacgctgttt tcaggcttag atctactcct tggcgattgg
     3061 gcaaaagccc tacgcattga tgtattgcat attgattcag gtttactact ggcaatttta
     3121 cccccggggg cttttattgg gcttggggtg atattggcgg tgaagaatat tattgataag
     3181 agatgatgtg tt
//
LOCUS       DANKBV010000186         3186 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.186, whole genome shotgun sequence.
ACCESSION   DANKBV010000186 DANKBV010000000
VERSION     DANKBV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.186"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            168..1466
                     /gene="purA"
                     /locus_tag="PWY43_002329"
     CDS             168..1466
                     /gene="purA"
                     /locus_tag="PWY43_002329"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL2137162.1"
                     /translation="MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTL
                     IINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEALMKEMGELEERGINVRERLKISE
                     ACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEHFAA
                     KLKDILDYYNFQLVNYYKVEAVDYQKTLDDVFAIADVITGMVADVTTLLHEARENGDN
                     ILFEGAQGTMLDIDHGTYPFVTSSNTTAGGVATGSGYGPRHLDYVLGIIKAYCTRVGS
                     GPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLD
                     VLDGFKELKICVAYKMPNGKIVEYAPLAAKDWEGVEPIYETMPGWSENTFRVTKREDL
                     PQAALDYIKRIEELVGVPVDILSTGPDRVETMILRDPFAA"
     gene            complement(1626..1702)
                     /locus_tag="PWY43_002330"
     tRNA            complement(1626..1702)
                     /locus_tag="PWY43_002330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1666..1668),aa:Arg,seq:tct)
     gene            complement(1727..1803)
                     /locus_tag="PWY43_002331"
     tRNA            complement(1727..1803)
                     /locus_tag="PWY43_002331"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1767..1769),aa:Pro,seq:tgg)
     gene            1973..2833
                     /gene="folD"
                     /locus_tag="PWY43_002332"
     CDS             1973..2833
                     /gene="folD"
                     /locus_tag="PWY43_002332"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDL2137163.1"
                     /translation="MTAQIISGSEVSSQIKKKVSEKIAHYVEQGKRNPGLAVILVGND
                     PASQVYVGSKRKSCAEIGIESKSYDLPEETTEAELLALIEQLNQDDSVDGILVQLPLP
                     KQIDSTKVIEAILPHKDVDGFHPYNVGRLSQRIPTLRPCTPYGVMKLLETTGVDLYGQ
                     HAVIVGASNIVGRPMALELLLGGCTVTVTHRFTKDLEHHVRQADILVVAVGKPEFIPG
                     EWVKDGAIVIDVGINRGADGKLKGDVEYSVASQKAAFITPVPGGVGPMTVAMLMQNTL
                     QAYENHLQVV"
     gene            2948..>3186
                     /locus_tag="PWY43_002333"
     CDS             2948..>3186
                     /locus_tag="PWY43_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991094.1"
                     /note="catalyzes the conversion of uridine to
                     4-thiouridinine tRNA; also required for the synthesis of
                     the thiazole moiety; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2137164.1"
                     /translation="MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNVLLKETENVSVV
                     RHWDFIEVRAKVEEETALVLDLLKRTPGIHHILEV"
BASE COUNT          926 a          568 c          743 g          949 t
ORIGIN      
        1 ctataaaacc cgatcttgta tcgggttttt aattttcgtc cctattctga tcatagcaag
       61 cggtcaattt tggctgattt tttgcaaaaa atttgaaaaa attgaccgct tgcgatatga
      121 agttataatg ggtttcgtta ttcatcctaa ctaaaagagg catttctatg ggtaaaagtg
      181 ttgctattct tggcgctcag tggggcgatg agggaaaagg caaaattgtt gatttattaa
      241 cagatcgtgt gaaatatgta gtacgttatc aaggtggaca taatgcaggg cataccttaa
      301 tcattaatgg tgaaaaaacc gttctgcgtt taattccatc gggtattttg cgtgataacg
      361 tgacttgttt aatcggtaat ggtgtagtgc tttctccaga ggcattaatg aaagagatgg
      421 gggagctaga agaacgaggc attaatgtgc gtgagcgttt aaaaatttct gaagcttgcc
      481 cgttaattct gccttatcac gttgcgatgg atcacgctcg agaagcggca cttggtaaaa
      541 acaaaatcgg tacaacagga cgtggtattg gcccagctta tgaagataaa gtcgctcgcc
      601 gtggtttacg tgtcagcgat ttattcgaca aagaacattt tgccgcaaaa ttaaaagata
      661 ttttagatta ctacaacttc caattagtca attattacaa agttgaagcg gtagattatc
      721 aaaaaacctt agatgatgtg tttgcgattg cggatgtgat taccggaatg gttgccgatg
      781 tgacaacttt acttcacgaa gcacgtgaaa acggcgacaa tattttattt gaaggggctc
      841 aaggcacgat gttagatatc gaccacggta cttacccgtt cgtaaccagc tcgaatacga
      901 cagcaggcgg tgtggcaacc ggatcaggct acggtcctcg tcatttagat tatgtattag
      961 ggattatcaa agcctactgt acccgtgtag gtagtggacc gttcaccacc gaactatttg
     1021 acgatgtagg tgctgaaatt gctcgcaaag gtaatgagtt cggagcggta accggtcgcc
     1081 ctcgccgttg tggctggttt gatgccgtag ccgttcgccg agcagtgcaa atcaactcca
     1141 tttcaggctt ctgtatgact aaactcgatg ttttagacgg attcaaagag ctaaaaatct
     1201 gcgttgccta caaaatgcca aacggaaaaa ttgtcgaata tgcccctctt gcagcaaaag
     1261 actgggaagg tgttgaacca atttatgaaa caatgccggg ttggtctgaa aatactttcc
     1321 gtgtgacaaa acgtgaagat ttaccacaag ccgcattaga ttacattaaa cgtatcgaag
     1381 agctggtcgg tgtgccggta gatattcttt ctacaggtcc ggatcgtgtc gaaacgatga
     1441 ttttgcgtga tccttttgca gcttaataaa agataaaata aagccattta gtgagataga
     1501 gctagatggc ttttttgttt gaaccagtaa actttacttt ggcacaattt tgtcacaata
     1561 cagaaaatag ttctttagcg tgagttttta aggctgtgta agtatttgaa tttaaaagaa
     1621 tttaatggca cgccctaaag gattcgaacc tttgacccac gccttagaag ggcgttgctc
     1681 tatccagctg agctaagggc gcagatgaag ataatataaa aagaagtggt cggcgagata
     1741 ggatttgaac ctacgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg
     1801 ccgacataag atgttgttga ttatagattt ctttgcacaa cagtcaatct ttttttgtga
     1861 atgtcggctt aaatgattga agtttatccg ttattcaaaa ttagcttatc aaaaaggcta
     1921 ttttttgaga aaatgacgaa tgttttgcca agtgatattt cgggaggtag taatgactgc
     1981 acaaattatt tctgggagtg aggtttcttc acaaattaaa aaaaaagtga gtgagaaaat
     2041 cgctcattat gttgaacagg gtaaacgtaa ccccggtctt gcggtcattt tagtggggaa
     2101 tgacccagcc tctcaagtgt atgtggggag taaacgcaaa agttgtgctg aaattgggat
     2161 tgagtcgaaa tcttatgatt tacctgaaga aactacagag gctgaattat tagctctgat
     2221 tgaacagctg aatcaagatg attcggtcga tgggatttta gttcaattac cgttacctaa
     2281 acaaattgat tcgactaaag tgattgaagc catcttacca cataaagatg ttgatggatt
     2341 tcatccttat aatgttgggc gtttatctca gcgtattccc actttacgcc cttgcacgcc
     2401 atacggtgtg atgaagttgc ttgaaacaac aggggtagat ttatacggtc aacacgcagt
     2461 gattgtaggt gcgtctaata ttgttggtcg cccgatggcg ttagaattgt tgcttggtgg
     2521 ttgtacagtc actgtcactc atcgctttac caaagattta gagcatcacg ttcgtcaagc
     2581 agatatttta gttgtagcag tcggcaagcc tgaatttatt cccggggagt gggttaaaga
     2641 cggtgctatc gtgattgatg tagggattaa ccgtggagca gacggcaaac taaaaggtga
     2701 tgtagagtat tcagttgcct cgcaaaaagc ggcatttatt acccctgttc ccggtggtgt
     2761 aggcccgatg acggtggcaa tgttaatgca aaataccttg caggcgtatg aaaaccattt
     2821 acaagtggtc taatttgaca gatattttgc aaatcctcgg cattggtcga ggatttcttt
     2881 ttctaggctt tttcagcaga aaaggctaaa atactcgcca tttttgtcac actattctga
     2941 tttaaatatg aaatttatca tcaaactttt ccctgaaatt atgattaaaa gcgattctgt
     3001 tcggaaacgc tttatcaaaa ttttaacctc aaatattcgt aacgtattat taaaagagac
     3061 tgaaaatgtg tctgtggttc gccattggga ctttattgaa gttcgggcga aagtggagga
     3121 agaaacggca ttagtgctgg atttacttaa acgcacgccg ggtattcacc atattttaga
     3181 agtgga
//
LOCUS       DANKBV010000187         3163 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.187, whole genome shotgun sequence.
ACCESSION   DANKBV010000187 DANKBV010000000
VERSION     DANKBV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.187"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..492)
                     /locus_tag="PWY43_002334"
     CDS             complement(<1..492)
                     /locus_tag="PWY43_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDL2137165.1"
                     /translation="MANSVMIVQGVEVKVTARDGEDYISLTDMCKAFGDSDQLIKNWL
                     QNKNTIEFLQVWEELNNPNFNLVELHQIKNNIGLNRFVMSVKKWTATGAIGLVAKAGR
                     YGSGTYAHKDIALEFGSWLSPEFKLYLIKEFQRLKQQEANESKLEWSVKRILSKANYR
                     IHTD"
     gene            722..1099
                     /locus_tag="PWY43_002335"
     CDS             722..1099
                     /locus_tag="PWY43_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137166.1"
                     /translation="MFSLLRDALTFAAMFTGILFPAIIHKYDLSLGTQIQLLSFVPAF
                     FVLCHFLIKRKYQRDFDRLSYLSAVVCVAMCAGVGAFSQSEMIELGFKVERVLDYVTI
                     KFLLYFWSLLLLPVVLRKLFGKD"
     gene            1231..1419
                     /locus_tag="PWY43_002336"
     CDS             1231..1419
                     /locus_tag="PWY43_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137167.1"
                     /translation="MKELFKQWLINQDSPFINSCGVECILSKVDDRLNIINANEEETE
                     TLIEWRNAFLQDVSVFIA"
     gene            complement(1423..1887)
                     /locus_tag="PWY43_002337"
     CDS             complement(1423..1887)
                     /locus_tag="PWY43_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2137168.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCGGIARN
                     NPEMIKDAIGDLIVVCIIKAKQEKQPIYPVQFENHRLNGYRFDISVAVHCLYDIERIK
                     DRGKYSRFFGILHHLALINRFTLNDCLAHAYDQIKDRKGEMQAGIWVKEDDL"
     gene            complement(1871..2074)
                     /locus_tag="PWY43_002338"
     CDS             complement(1871..2074)
                     /locus_tag="PWY43_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137169.1"
                     /translation="MTDTERLDFIAQRKLDVRYSDLVDEFIIASYTKPHEQYFHWARN
                     ADLRTAIDEAMKEMRGLNNATTN"
     gene            complement(2071..2562)
                     /locus_tag="PWY43_002339"
     CDS             complement(2071..2562)
                     /locus_tag="PWY43_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL2137170.1"
                     /translation="MNEDKFFSVDVSNDIHILNLHETLEQAKQSCLNGATEAYEFADD
                     MDDHESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERESELFGEAEQVIEPPTLVENNG
                     WISVKDRLPEIRKEVLITNNFIMIAQFNGESWFKPGGFLGIQPVYGVTHWQPLPEPPK
                     EEK"
     gene            complement(2607..3059)
                     /locus_tag="PWY43_002340"
     CDS             complement(2607..3059)
                     /locus_tag="PWY43_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2137171.1"
                     /translation="MKPFDLNKALAGEPVKLRNNDKAFVKYLISDDYIRDNKDHQVQG
                     YTVDEENVFLSEVSWAVSGSHFNDGTIAQYDIVGMWEEPRPTVTLTLPCPLKEPRDGM
                     WFIGDNFNVIKSNFPTHSYIEKLFDQGLYFASAEDAGAWLDALKNSMR"
BASE COUNT          872 a          617 c          572 g         1102 t
ORIGIN      
        1 atcagtatga atgcggtagt tcgccttgct taaaatgcgt tttacgctcc attcgagctt
       61 actttcgttc gcttcttgct gtttgagtcg ttggaactct ttgatgagat aaagcttaaa
      121 ttctggactg agccaagaac caaattcgag agcaatatct ttgtgggcgt aagtgccgct
      181 accatatcgt cctgcttttg ctactagccc aatagctccc gttgcagtcc attttttgac
      241 agacatcaca aagcggttta agccgatgtt gtttttaatt tggtgtaatt ccaccaaatt
      301 aaaatttgga ttattcagct cttcccaaac ttgaagaaat tcaatggtat ttttattttg
      361 aagccagttt ttaatgagct ggtcactgtc tccaaaagct ttgcacatat cagttaggct
      421 gatgtaatcc tcaccatcac gagcggttac tttgacctcc acgccttgta caatcattac
      481 gctatttgcc atttcgtttc tccgttttta ggtacaaaaa aagccgttgg tggacggctt
      541 gaaagtgcgg ttatcttaat ccgaattggg ggcggtgtca attagattaa tccgtataag
      601 tatggaacca agttaaagag tagaactaca acacataacc aaaaaatgaa attcatatct
      661 tgctcagttt tgattttgtg taaaatacgc ttaagtttgt ttttcattta ggaaaccttt
      721 aatgtttagt ttattgcgtg atgctttaac ctttgctgca atgtttacag ggattttatt
      781 tcctgcaatt attcataaat acgatctatc acttggtaca caaatccaat tacttagctt
      841 tgttccagct ttctttgtgt tgtgccattt tcttattaag cgaaaatatc aacgtgattt
      901 tgacagactt tcgtatttat ctgctgtggt ttgcgtagca atgtgtgcag gcgttggagc
      961 atttagccaa tcagaaatga ttgagctagg ttttaaagtt gagcgtgttc ttgattatgt
     1021 gactatcaaa ttcttactct acttttggag cttgctgtta ttgccagttg tgctaagaaa
     1081 attgtttggg aaagattagt tggttattta aagagagcta tttgtcaaac ctcaatatct
     1141 atttttgcga tctgcatcga aaaaataaat tttcgttctg acagtaaaac gctttcgtag
     1201 taatctcata aaaaacataa ggggaacact atgaaagagc ttttcaaaca atggctaatc
     1261 aatcaggact cgccattcat taatagctgt ggcgttgagt gtattttgtc aaaagttgat
     1321 gaccggctaa acataatcaa cgccaacgaa gaagaaaccg aaactcttat agagtggcgg
     1381 aatgcttttc tgcaagatgt ttcggttttt atagcttagg tactataaat cgtcttcttt
     1441 aacccaaatc ccagcttgca tttcgccctt gcgatctttg atttggtcgt aggcgtgagc
     1501 gaggcagtca ttaagcgtaa atctatttat taaggctaaa tggtgtaata tgccaaaaaa
     1561 acggctatat ttacctctgt ctttaatgcg ttcaatatca tatagacaat gaacagcgac
     1621 cgaaatatca aatctgtaac catttaatct atgattttca aattgtacag ggtaaatagg
     1681 ttgtttttct tgttttgctt taataataca gacaacaatc aaatccccaa tcgcatcttt
     1741 aatcatttca ggattattgc gggcaatgcc tccgcagagt tcgccaaact cttccatcag
     1801 tttgagcatt tgtttttgtg gggttgagcc gttaattaaa ttgcggtctt ccgcccattg
     1861 ttcgatgttt ttaattagtt gttgcattgt ttagtcctct catttctttc attgcttcat
     1921 caatcgccgt gcgtaaatcg gcatttcttg cccaatgaaa atactgctcg tgcggtttgg
     1981 tatagctagc aataataaat tcgtccacca aatcagaata tcggacatct aatttgcgtt
     2041 gggcgataaa gtctaagcgt tcggtgtcgg tcatttttcc tcctttggtg gttcgggaag
     2101 tggttgccag tgggtaacac catagacagg ctgaatacct aagaatccac ccggcttgaa
     2161 ccagctctcg ccattgaatt gagcaatcat aataaaattg tttgttatca agacttcttt
     2221 gcggatttct ggcaatctgt ctttgactga aatccagccg ttgttttcta caagtgttgg
     2281 tggttcaata acttgttcag cttccccaaa taactctgat tctctttctt catcggttag
     2341 tgggcggata tctgattttg ctctacctaa aacaacacca taaactgcat agggtaaatc
     2401 ataggcttca tagctttcgt gatcatccat gtcatccgca aactcatagg cttcagtagc
     2461 accatttaaa cagctttgtt ttgcttgctc taaggtttcg tgcagattta ggatatgtat
     2521 atcatttgat acatccaccg aaaaaaattt gtcttcattc attttctttc tccaaaatac
     2581 caatagttga gagaataata agcatactat ctcatgctat tcttaagtgc atctaaccac
     2641 gctccagcat cttcggctga agcgaaatat aacccttgat caaatagttt ttctatataa
     2701 gaatgtgtag ggaaatttga ttttatcaca ttgaaattat cgccaataaa ccacattcca
     2761 tctcttggtt cttttaatgg acaaggaaga gtgagagtta ctgtaggtct tggttcttcc
     2821 cacataccaa caatatcata ttgagctatt gttccatcat tgaaatgaga tccgctaaca
     2881 gcccacgata cttctgacaa aaatacattt tcttcatcaa ctgtataccc ttgtacttgg
     2941 tggtctttat tatctctaat atagtcatca ctaattaggt attttacaaa tgccttatca
     3001 ttatttctta gttttactgg ctcacctgcc aatgctttat ttaaatcaaa tggtttcatt
     3061 ttattctcca ataaaaaaga gactgattaa gtcgcttctc ctaaaattct gctttttgct
     3121 acctcaatca gtagctgata ttcccgcttc gttttctcat cgt
//
LOCUS       DANKBV010000188         3163 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.188, whole genome shotgun sequence.
ACCESSION   DANKBV010000188 DANKBV010000000
VERSION     DANKBV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.188"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..3057
                     /gene="putA"
                     /locus_tag="PWY43_002341"
     CDS             <1..3057
                     /gene="putA"
                     /locus_tag="PWY43_002341"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HDL2137172.1"
                     /translation="IQQAIKNGEKRFAMGYRYSYDMLGEAALTAEDAKRYYNDYVASI
                     HAVGATSKGLGVYKSSGVSVKLSAIHPRYSLSQHQRVMDELYPRLKELFLLAKKYDIG
                     LNIDAEEADRLELSLDLMDRLLADPDLAGFNGIGFVVQAYQKRCPYVIEHLIETARKH
                     NRQLMIRLVKGAYWDTEIKRAQMDGAEGYPVFSRKVHTDLNYIVCAKKLLAAQDVIYP
                     QFATHNAQTLATIYHLAQGKRFEFQCLHGMGESLYDQVVGSDNLNVQCRIYAPVGTYQ
                     TLLAYLVRRLLENGANSSFVSQIVDENLSVDDLAQDPVQKAELTNGTMHPKIPLPVDL
                     FDDNRVNSKGYDLTDAVSLQNLENALNELTKQHYRAEPQLANDNTSGQESRKIYNPAN
                     QAELVGEVIDATENDMEQAFESAAAFQGEWANTSPAVRAKCLEKMADLMEANMPQLFD
                     LAVREAGKTLNNAIAEVREAVDFCRYYAKEVRNNIEAYKNPRGIVVAISPWNFPLAIF
                     VGEVSSALVAGNVVLAKPAEQTSLMAHYAASLFYQAGVPKAALQCLPGRGKTVGVALV
                     ADHRVNGVIFTGSTETAKNINRNLHKNEKIIPLIAETGGQNAMIVDSSALGEQVVIDV
                     LNSAFDSAGQRCSALRVLYLQRDVADHILTMLKGAMAELKVGLPQHLTTDVGPVIDKV
                     AQQRLLDHIENLKKSAKSYYQVAISSEIAENGIFVPPTLLEIDHISQLKHEVFGPVLH
                     VIRFDADKLDQVIADINSTGFGLTSGLHSRIDETVDLWLKSVHAGNLYVNRNTVGAVV
                     GVQPFGGMGLSGTGPKAGGPLYLQRLVNLQEWTLNGLQGNAQAVDFDLNFAIRKVLSG
                     AEQQTALDLAKSLARRSPLGKTYPMQGITGEKNEMIFVARPQIGIGKGDLASQVQALI
                     AIVTVGSQAVVEKSSELAKHASAFGKKLQVVDDFNSLDHLSTLIVLDLLSSEEKLHHA
                     ERKGAILTYIEGLDLALSLLPLVHEKAISINTAAAGGNASLMSEMD"
BASE COUNT          889 a          715 c          788 g          771 t
ORIGIN      
        1 attcaacagg cgattaaaaa cggcgaaaaa cgcttcgcaa tgggttatcg ttacagctac
       61 gatatgctgg gcgaagcggc actcaccgcc gaagatgcca aacgctatta caacgactat
      121 gttgcctcta ttcacgctgt gggtgccacc tcgaaaggct tgggcgtgta taaatcctcg
      181 ggtgtgtcgg tcaaactctc cgccattcac cctcgttatt ccctttccca acaccaacgt
      241 gtgatggacg aactctatcc acgtctgaaa gaactgttct tgctggcgaa aaagtatgac
      301 atcggcttga acattgatgc cgaagaagcc gaccgcctcg aattgtcgct tgatttgatg
      361 gacagactgc ttgccgaccc tgatttggca gggtttaacg gcattggttt tgtggtgcag
      421 gcgtaccaaa aacgttgccc gtatgtgatc gagcatttaa ttgagaccgc ccgtaaacat
      481 aatcgccagc taatgattcg cttggtaaaa ggcgcatatt gggacactga aatcaagcgg
      541 gcgcaaatgg acggtgcaga gggctatccg gtcttctccc gtaaggtgca taccgatttg
      601 aactacatcg tttgtgccaa aaaattgctg gcggcgcaag atgtgatcta tccgcaattt
      661 gccacccaca acgctcaaac tctcgccacc atttaccact tggcacaggg caaacgtttt
      721 gagtttcaat gcttgcacgg tatgggagag agcttgtacg accaagtggt tggcagcgat
      781 aatcttaacg tgcaatgtcg catttatgcc cctgtcggca cttatcaaac cctgcttgcc
      841 tatttagtac gtcgcttgtt ggaaaacgga gcgaacagct catttgttag ccaaattgtg
      901 gatgaaaatc tcagtgtaga tgatctggct caagaccctg ttcaaaaagc agaactgact
      961 aatggcacta tgcacccgaa aattccattg ccagtcgatt tatttgacga caatcgtgtt
     1021 aattccaaag gctatgattt aactgatgca gtaagcctac aaaatttaga aaatgccctc
     1081 aatgaactta ccaaacagca ttaccgtgct gaaccacagc tggcaaatga taatacaagc
     1141 ggtcaagaaa gccgcaaaat ttacaatcca gcgaaccaag ctgaattagt cggcgaagtg
     1201 attgatgcca ccgaaaacga tatggaacag gcgtttgaaa gtgcggcggc gttccaaggt
     1261 gaatgggcga atacttcccc tgcggtgcgt gccaagtgct tagaaaaaat ggcggacttg
     1321 atggaagcca atatgccaca actgttcgat ttggcggtgc gtgaggcagg caaaacgctc
     1381 aacaatgcaa ttgctgaagt gcgtgaagcc gtcgatttct gtcgttatta tgccaaagag
     1441 gtacgcaaca acattgaagc atacaaaaat ccgcgtggca ttgtagtagc aattagcccg
     1501 tggaactttc cgttagcgat ttttgtgggt gaagtttcat ctgcgttggt ggcaggcaat
     1561 gtggtactag caaaaccagc tgaacaaact tcgttaatgg cacattatgc tgccagcctt
     1621 ttctaccaag caggtgtgcc gaaagcggcg ttgcaatgct taccgggtag aggaaaaacg
     1681 gttggggtgg cgttggtggc agatcatcgt gtgaacggcg tgatttttac tggttcaaca
     1741 gagacagcta aaaacattaa ccgcaatttg cacaaaaacg agaaaattat accgcttatt
     1801 gccgaaactg gcggacaaaa tgcaatgatt gttgatagct cggcactggg cgagcaggtt
     1861 gtgatagatg ttttaaactc cgcatttgac tcagcagggc aacgttgttc ggcattgcgt
     1921 gtgctgtatc tgcaacgtga tgttgccgat cacattctca caatgctcaa aggggcaatg
     1981 gcggagttga aagttggtct tccacaacac ttaactaccg atgtcggacc agtaatcgat
     2041 aaagtcgcac aacaacgctt gctcgatcat attgaaaacc tcaaaaaatc ggcgaaaagt
     2101 tactatcagg tggctatctc ctctgaaatt gccgaaaatg gtattttcgt accgccaacg
     2161 ctgttagaaa tagatcatat cagccagctt aaacacgaag tattcggacc ggtgctacac
     2221 gttatccgtt ttgatgcgga taaactcgac caagtgattg ccgacataaa ctccacaggt
     2281 ttcggtctaa ccagcggttt gcatagccga attgatgaaa cggtggattt atggttgaaa
     2341 tctgtccacg ctggaaatct gtatgtaaac cgtaacacgg taggggctgt ggtcggcgta
     2401 cagccattcg gtggaatggg gttatcagga actgggccga aagctggcgg gccgctctat
     2461 ctacaacgtt tagtaaacct ccaagaatgg acattgaacg gtttacaggg caatgcacaa
     2521 gcggtcgatt ttgacttaaa ttttgcaatc cgcaaggttc taagtggagc tgagcagcaa
     2581 acggcgttag atctggccaa aagccttgca cgccgctcgc cactcggaaa aacctaccca
     2641 atgcagggta ttaccggcga gaaaaacgag atgattttcg tggctcgccc acagattggg
     2701 attggcaaag gtgatttggc ttcacaggtg caagccttga ttgccattgt aacggtcggc
     2761 agccaagcgg tggtagaaaa atcaagcgag ctggcaaaac acgcctctgc ctttggtaag
     2821 aaattacagg tggtggatga tttcaacagt ctcgatcacc tcagcacact cattgtgctt
     2881 gacctgcttt ctagtgagga aaaattgcat catgccgaac gcaaaggggc gattttgacc
     2941 tacatcgaag ggctggattt agcgctctca ctgctgccgt tggtacacga gaaagcaatc
     3001 agcatcaaca ccgctgccgc agggggaaat gccagcttaa tgagtgaaat ggattaagca
     3061 acaaacaaaa gagtataaga gggcagatca caatgatctg cctttttatt ttaaggggct
     3121 gtcctagata acgactaaaa cttccattag gttaagataa ccc
//
LOCUS       DANKBV010000189         3109 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.189, whole genome shotgun sequence.
ACCESSION   DANKBV010000189 DANKBV010000000
VERSION     DANKBV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.189"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..262)
                     /locus_tag="PWY43_002342"
     CDS             complement(<1..262)
                     /locus_tag="PWY43_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL2137173.1"
                     /translation="MTDFDKDTYQTPKYFRNWLNHRYAWFHIDGCSNGKNALYSYWIG
                     KAAEGLDEDTLSGQIAEDFLAENLLDVILERVADWGELLRIFV"
     gene            complement(259..906)
                     /locus_tag="PWY43_002343"
     CDS             complement(259..906)
                     /locus_tag="PWY43_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2137174.1"
                     /translation="MSLQKTEQNRPLAVLGEQQGKLSGEVESLVDRIFDQLLASCPSI
                     QYWSEKQVATAKKQWILGFAENGIRTVEQVRQGMKALRAKTDDFVPSIGKFIQWCNTI
                     DYHELGLPDLEQLLKLLDRSLRFDFIEIQKLKFKSNAEYWLITDLYARNKKYSWKDET
                     LRNQAEKALVAMAKRIMSGEQIPPPKPLIEEKKKVPIDPRIQRILDEKKQAGAMQ"
     gene            complement(906..1190)
                     /locus_tag="PWY43_002344"
     CDS             complement(906..1190)
                     /locus_tag="PWY43_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137175.1"
                     /translation="MLLEQFGITGKLAEDFIVHRKAKKAPITETALNGYQREADKAKI
                     PIQKAVEIAIERGWTGFKADWQWQDDQPKHRPKDNMRAEWNNPEAWAEVF"
     gene            complement(1328..1679)
                     /locus_tag="PWY43_002345"
                     /pseudo
     CDS             complement(1328..1679)
                     /locus_tag="PWY43_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1676..1918)
                     /locus_tag="PWY43_002346"
     CDS             complement(1676..1918)
                     /locus_tag="PWY43_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2137176.1"
                     /translation="MYGINLNLNEKSSQTQNARILAHLQNGGRITSLSALDDFGCLRL
                     SARIKDLRDRGHDIQSVFITVPNGKKVKQYFMECAK"
     gene            complement(2075..2308)
                     /locus_tag="PWY43_002347"
     CDS             complement(2075..2308)
                     /locus_tag="PWY43_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2137177.1"
                     /translation="MEELKAYLNSLTTKEQESFALRCNTTVGYLRKSISRNSRLGAEI
                     CVAIERETKGLVTRKHLIEDWHLRWPELANDKQ"
     gene            2437..>3109
                     /locus_tag="PWY43_002348"
     CDS             2437..>3109
                     /locus_tag="PWY43_002348"
                     /inference="COORDINATES: protein motif:HMM:NF012920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2137178.1"
                     /translation="MNELRLLRQKKLKRLIDEVSEGNIADFARKIGKEPSYVARMLYP
                     SDKAGAKPIGEKMALHICHKFVLPLDWFNRDDDGEFIQINNGEEDNIILSVLNIQASA
                     GIGAKNGDVMEVIREIKFNPEQFYNLFRGITPEHIELISVKGDSMEPTFSHGDLLFVD
                     VTIQEYDGDGVYVFTCDDYIFVKRIQKTGRHFTILSDNDRYKPWQLDQEIYIHGKVKV
                     HQSQQL"
BASE COUNT          884 a          640 c          575 g         1010 t
ORIGIN      
        1 taacgaagat acggagtaat tcgccccaat ctgccacacg ttccaagatc acatcgagca
       61 agttttctgc caagaaatcc tcggcaatct gaccacttag cgtgtcttcg tccaagcctt
      121 ctgccgcttt gccaatccag tagctataca atgcgttttt gccgttagaa caaccgtcaa
      181 tgtgaaacca cgcatagcga tgatttagcc aattacggaa atactttggc gtttggtaag
      241 tgtctttatc aaaatctgtc attgcattgc tcctgcttgt ttcttctcgt ccaaaattcg
      301 ctgaatgcgt ggatctattg gcactttctt tttctcttcg attagcggtt taggtggtgg
      361 aatttgctct ccgctcataa ttcgttttgc cattgccact aaggcttttt ctgcttgatt
      421 acgtaaggtt tcatctttcc aactatattt cttgttacgt gcgtataggt cagttatcaa
      481 ccaatattca gcgtttgatt taaatttaag tttctgaatc tcaataaaat caaatcttaa
      541 agagcgatcc aaaagcttga gtaactgctc taaatcaggt aatcctaatt cgtgatagtc
      601 aatcgtattg caccactgga taaatttgcc gatactcggg acgaaatcat cagtttttgc
      661 tcttaacgct ttcataccct gtcgaacttg ctcaaccgtg cggattccgt tctccgcaaa
      721 gcctaaaatc cattgttttt tggctgtggc aacttgcttt tccgaccagt attgaatact
      781 tgggcaactc gcaagcaact ggtcaaatat tctgtccaca aggctttcaa cctcaccgga
      841 taatttgcct tgctgctcac ctaacaccgc aagcggtcga ttttgctctg ttttttgcag
      901 gctcatcaaa acacctccgc ccacgcttca gggttattcc attcagctcg catattgtct
      961 tttgggcgat gtttaggttg atcgtcttgc cactgccaat cggctttaaa tcctgtccag
     1021 ccacgttcaa tcgcaatttc taccgccttt tgaatcggga ttttggcttt gtctgcctct
     1081 cgctgatagc cattcagtgc agtttcggta atcggggctt ttttggcttt gcggtgtacg
     1141 ataaaatcct ccgccaattt gcctgtaatg ccgaattgtt caagcaacac caacacagca
     1201 gattttttag tctcacgtgt gtgtgcgtta atacccgtaa gggtattatt atgatcatta
     1261 gtatttttat aattagtatt attatttgtc ggatttaaat ccgagctttt tcggatctgt
     1321 ttccgagcta actcggattt atttccgagt gtcggattta aatccgagtt aaactcattc
     1381 caagtcttcc ctttttcagt taatcgaata aggtctttat ctccgtgttt ttgatacacg
     1441 atcaaccctt ttttgtgaaa ctcgacaaaa tggcgataga cggtatcagg ctttgagtaa
     1501 aacaaaggta gttcttcgat caccgcatta cgggaaaccc agtaataaac cacgccatca
     1561 ataatgattt ctttcgccca agcaggagct tgattcagta aatcaaataa tgctccttga
     1621 ttagcattta agttccattc aaggcatttt gcattgttga tgtaagtgct aaatctcatt
     1681 tcgcacactc cataaaatac tgcttcactt tcttgccatt cggcactgta ataaatacgc
     1741 tttgaatatc gtgaccacgg tcacgcaaat ctttaatgcg agcggataaa cgcaagcagc
     1801 cgaaatcgtc taacgcagaa agcgaagtaa tacgaccgcc attttgaaga tgtgccaaaa
     1861 ttcttgcatt ttgggtttga cttgattttt cgtttaggtt aagatttatc ccatacatat
     1921 aggaaacctc cttagtaatt agccaccgtt acagcggtgg tttttttaat tgttaaaaac
     1981 ccaccacgca agcagcataa aaattgccac tctaataaaa attgaatttt tactgtggta
     2041 gaatgatttg agttttttag aaaaaacttt ccctttattg cttgtcattt gccaattccg
     2101 gccagcgtaa gtgccaatct tcaattaaat gctttctagt aactaaacct tttgtttcac
     2161 gctctatcgc aacgcaaatt tccgcaccta atcgagaatt tcttgagatc gattttctta
     2221 aatacccaac tgtcgtatta caacgcaagg caaaactctc ttgctctttt gtggttaaag
     2281 aattgaggta tgcttttaat tcttccattg tgagtcctct tttaaaaacg ccaaaataat
     2341 accattaggt aaaaataata tcaataccat ttggtttgtt taccttttag taaataagag
     2401 tacaattact aaaaagtaat ccttagagga ggttatatga atgagttgag acttcttaga
     2461 caaaaaaaat tgaaacgcct aattgatgaa gtgagtgagg gaaatattgc tgactttgca
     2521 agaaaaatag gtaaagagcc tagctatgta gcaagaatgt tgtatccgtc cgataaagct
     2581 ggagctaagc ctattggtga aaaaatggca ctccatattt gccataaatt tgtgctaccc
     2641 cttgattggt ttaatcgaga tgatgacggc gaatttattc aaatcaacaa tggtgaggaa
     2701 gataatatca tcttaagtgt attgaatatt caagcatcgg ccggtatagg agcaaaaaac
     2761 ggtgatgtga tggaagtcat tagagaaatt aaattcaatc cagaacaatt ttataaccta
     2821 tttagaggta ttacgcctga acatatcgag ctgatcagtg taaaaggcga ttctatggag
     2881 ccaacattct cacacggtga tttgcttttt gttgatgtaa ctatccaaga atacgatggc
     2941 gatggtgtgt atgtttttac ttgcgatgac tatatttttg taaaaaggat tcaaaaaaca
     3001 ggcagacact ttactatttt atctgataat gaccgctata aaccttggca attagatcaa
     3061 gaaatttata ttcacggcaa agttaaagtc caccaaagcc aacagttga
//
LOCUS       DANKBV010000190         3094 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.190, whole genome shotgun sequence.
ACCESSION   DANKBV010000190 DANKBV010000000
VERSION     DANKBV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.190"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            122..844
                     /gene="recO"
                     /locus_tag="PWY43_002349"
     CDS             122..844
                     /gene="recO"
                     /locus_tag="PWY43_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2137179.1"
                     /translation="MTTDQWQRAFVLHRREYSESSLLVEFFTEHHGRITLLAKGARRA
                     RSPLKAVLQPFTPLLVRWSGKGDLKTLTKAEPAALTLPMETAALYSGFYINEVLARVL
                     ENQTAYPELFQHYLRCMTELATQHQIEPILRTFEFHTLKALGYGVDFTHCTATGLPID
                     PKMSYQFRESEGFIASLLQNNNSFLGCDLLAFAQLDFSEKQTQQAAKRFTRIALKPYL
                     GSQPLKSRELFQSILPNKVKSG"
     gene            921..2156
                     /gene="rhlB"
                     /locus_tag="PWY43_002350"
     CDS             921..2156
                     /gene="rhlB"
                     /locus_tag="PWY43_002350"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDL2137180.1"
                     /translation="MSNHLTEHRFIDFPIHTNVLNALNSKGFEFCTPIQAKTFPITLN
                     GNDIAGQAQTGTGKTLAFLIATFNHLLNNEIETAKNQPRALILAPTRELAVQIGADAQ
                     LFLPHCDLKLALAYGGDGYDKQIQAIEKGVDILVGTTGRVIDYVKQGIIHLDKIQVIV
                     LDEADRMFDLGFIKDIRYLMRKAPVPTERLTLLFSATLSAKVRELAYEDMDNAEYIEI
                     EPLQRTGHRIKEELFYPSNEDKMALLLTLLEEEWPDRCMIFANTKHKCEEIWQYLAAD
                     GHRIGMLTGDIPQKKRLSLLDNFTKGNLDILVVTDVAARGLHIPDVTHVFNYDLPDDK
                     EDYVHRIGRTGRAGESGHSISFACERYAVNLPAIEEYIGHQIPVSQYDKESLLTLPKV
                     NRSRSKKTFLAKTKKNSRN"
     gene            2215..>3094
                     /locus_tag="PWY43_002351"
     CDS             2215..>3094
                     /locus_tag="PWY43_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HDL2137181.1"
                     /translation="MSLYQIARTETGKVISLNAKMANRHGLIAGATGTGKTVTLRKLA
                     ESLSDDGVPVFLVDVKGDLSGLVQAGNFSGKIAERILQFNLGGEDYLKGYPVSFWDVF
                     GTSGIPLRTTISEMGPMLLSRLLNLNDTQGGLLNLVFRVADDRGLLLIDLKDLRALLK
                     FVADNAKEFQLEYGHISAASVGAIQRALLRLENEGADNLFGEPALDLHDWIQTRNGRG
                     VINMLNAEKLINSPRVYSAFLLWFMAELFENLPEVGDPEKPTFVLFFDEAHLLFDDAP
                     KALVDKIEQVVRLIRSK"
BASE COUNT          943 a          530 c          674 g          947 t
ORIGIN      
        1 gtttggaatt ttttcgttaa gcaaattcaa accaagcgaa gatagcggtg aagtagggtt
       61 gcctttcttt ggttactttc tttggcgaag caaagaaagt gacatatagt cgggattaat
      121 tatgaccaca gatcaatggc aacgagcctt tgtccttcat cggcgtgaat acagcgaaag
      181 cagtttatta gtggagtttt ttaccgaaca tcacgggcgg attactttac ttgctaaagg
      241 tgcaaggcgg gctcgctcgc cgttgaaagc cgtgcttcag ccttttacac ccttgttagt
      301 gcgttggtct ggcaagggtg atttaaaaac actcacaaag gcagagcctg ctgctcttac
      361 tttaccaatg gaaacggctg cactttatag cggtttttat atcaatgagg tgcttgcaag
      421 agtgttagaa aatcagaccg cttaccctga attatttcaa cattatttgc gttgtatgac
      481 ggagctggct actcaacacc aaattgagcc gattttgcgt acttttgaat ttcatacatt
      541 aaaagcatta ggctatggcg tagattttac ccattgtacg gcaacaggtt tgccaattga
      601 tccgaaaatg agttaccaat ttagagaaag tgaaggcttt attgcctcat tattacaaaa
      661 taacaatagc tttttaggct gtgatttatt ggcgtttgct caattagatt tttcagaaaa
      721 acaaacacaa caagctgcca aacgatttac ccgaattgca ttgaaacctt atttaggttc
      781 tcagccacta aaaagccgag aactttttca atctatattg cctaataaag tgaaaagcgg
      841 ttagttttgt taaatttttt tgcaaatttt aattaaaata taaccgcttg tagcaataac
      901 aaatatctca aggaaatatt atgtcaaatc atcttaccga acaccgtttt attgacttcc
      961 ccattcacac aaatgtactc aatgcgttga atagtaaagg cttcgagttc tgtacaccta
     1021 tccaagcaaa aactttcccg attacgctta acggaaatga tattgcagga caggctcaaa
     1081 caggcacagg taaaacgctt gcttttttaa ttgccacgtt caaccattta ttaaataacg
     1141 aaattgagac agcaaaaaat cagccaagag cattaattct tgcaccaact cgtgagctgg
     1201 cggtacaaat cggggcagat gcacagcttt ttttaccaca ttgcgatctt aaacttgccc
     1261 ttgcttatgg tggtgatggt tacgacaagc aaatacaagc catagaaaaa ggggtagata
     1321 ttttagttgg aaccacaggg cgtgtgatcg attatgttaa gcaagggatt attcatttag
     1381 ataaaatcca agtgattgtg ctagatgaag ctgatcgtat gtttgatctc ggttttatta
     1441 aagatattcg ttatttaatg agaaaagctc ccgtgcctac tgagcgttta acgttgctct
     1501 tttcagcaac tttatccgct aaagtacgtg agcttgctta tgaagatatg gataatgctg
     1561 aatatattga gattgaaccg ctacaacgta cagggcatcg tataaaagaa gagttatttt
     1621 atccgtctaa tgaagacaaa atggcacttt tattgacgtt attggaggaa gaatggccag
     1681 accgctgtat gatttttgcc aatactaagc ataaatgtga ggaaatttgg caatatttag
     1741 cagccgatgg tcatcggatt ggaatgctaa caggtgatat tccacagaaa aaacgcctat
     1801 ctctgttaga taattttacc aaaggtaatt tagatatttt agtcgtgact gatgtggcag
     1861 cacgtggatt acatattcca gatgttacgc atgtgtttaa ttatgattta cctgatgata
     1921 aagaggatta cgttcatcgt attggtagaa cgggtcgggc aggggagagc ggccattcaa
     1981 ttagttttgc ttgcgagcgt tatgcggtta atttgcctgc aattgaagag tatattgggc
     2041 atcagattcc tgtgagtcaa tacgacaagg aatccttact gactttaccg aaagtgaacc
     2101 gctcaagaag taaaaaaacg ttcttggcaa aaacgaaaaa gaatagccgt aattaagtga
     2161 taaattttta tatgatgaga acaaaaaatt aatttacctt taagagaatg aaagatgagt
     2221 ttgtatcaaa ttgctcgaac agaaacaggc aaagttattt cgcttaatgc caaaatggca
     2281 aatcggcacg gtttaattgc tggggctacg ggtacaggta aaacggttac tttacgcaaa
     2341 ttggcagaat ctttgagtga tgatggtgtg ccagtatttt tagtagatgt aaaaggtgat
     2401 ctttcgggct tggtacaagc gggtaatttt tcaggaaaaa ttgcagaacg cattttacag
     2461 tttaatttag ggggagaaga ttacttaaag ggctatccag tttcgttttg ggacgtattt
     2521 ggtacatcag gtatcccact tcgaaccact atttccgaaa tgggaccgat gttactttct
     2581 cgtttactca atttaaacga tacccaagga ggcttgttaa atctcgtttt tcgtgtggca
     2641 gatgacagag gtttattgct aattgattta aaagatttgc gtgcgttgct taaatttgtc
     2701 gcagataatg cgaaagaatt ccaactggaa tatgggcata tttctgcagc aagcgttgga
     2761 gctattcagc gtgcgttatt aaggcttgaa aatgaagggg cagataattt atttggtgag
     2821 cctgcattag atttgcacga ttggatccaa acccgtaatg ggcgtggtgt aatcaatatg
     2881 ttgaatgcag aaaaattaat taactcacca agagtgtaca gtgcattcct gctttggttt
     2941 atggcagagc tctttgagaa tttgccagaa gttggcgacc cagaaaaacc gacgtttgtt
     3001 ttattctttg atgaagctca tttattgttt gacgatgccc ctaaggcatt ggtggataaa
     3061 attgaacagg tggttcgttt aattcgctca aaag
//
LOCUS       DANKBV010000191         3039 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.191, whole genome shotgun sequence.
ACCESSION   DANKBV010000191 DANKBV010000000
VERSION     DANKBV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.191"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            204..1682
                     /gene="fruB"
                     /locus_tag="PWY43_002352"
     CDS             204..1682
                     /gene="fruB"
                     /locus_tag="PWY43_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDL2137182.1"
                     /translation="MLNLSAKNIRLNGSASNKEEVIRFVAAELVAKGNVAAGYEVGML
                     NRETQTSTFLGNGIAIPHGTLDTRDLVKETGVQIIQLPQGVEWGKGNTAYVVIGIAAK
                     SDEHLTLLRQLTTVLSDEEAAATLAKTQNLDEFIAVLSGKKNLPALSQELISLNIESS
                     SLITLSAINAAKLQEQGYVSQAFISDVVANKALHLGNNLFVVDSAQGNQANGIAIARN
                     TQGQTLLTVAETDNALQPLLTQLLKPEVRSQFAHFSAEQMLGLLKGESVASTPVTNVE
                     ASGNQVVGTFTIRNDNGLHARPAAVLVQTVKPFSSKISVENLDRNTAPASAKSAMKVV
                     ALGASQGHRLRFVAEGEDAQQAIEALQQAIINGLGESVSFVPAVADTIESIAVSAIPA
                     EETATAVDANTKEATFVIKNEHGLHARPSAVLVNEVKKFNAKIEVQNVDKNSPLVSAK
                     SLMKIVALGVTKGTTLRFVASGDDAEVALAAIGAAIEAGLGE"
     gene            1696..2640
                     /gene="fruK"
                     /locus_tag="PWY43_002353"
     CDS             1696..2640
                     /gene="fruK"
                     /locus_tag="PWY43_002353"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2137183.1"
                     /translation="MRIATVTLNPAFDLVGKLQRIEIGEVNTVETLGLYPAGKGINVA
                     KVLADLGTKLSVTGFLGEENQGDFVNAFKHNDLADEFYRINGKTRINVKITETEADVT
                     DLNFLGFEISESDWEKFVTLSHQWESKFDLVAVCGSLPRGVTPEKFASWLESLHRQGL
                     KVVLDTSNAALTAGLTAHPWLVKPNRRELEVLVGHSLNSIEEIISAAKQLRSRGIENV
                     IISMGEQGSLWLNHEGVLQARPPRCENVVSTVGAGDSMVAGLIYGLSQGWSKEQTLRF
                     ASATSALAVVQSNVGIADRATLDDIIKQTDITVISENH"
     gene            2666..>3039
                     /locus_tag="PWY43_002354"
     CDS             2666..>3039
                     /locus_tag="PWY43_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616804.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIBC"
                     /protein_id="HDL2137184.1"
                     /translation="MNISFVFPQTLGNARRFLVNEMLSAAAKQQGHNVVAANQADFVV
                     LFDNAIPQEAAGKQGAILDLEQAFAQPEASLKQAVENAQTFANATATTAVSAVSSGVK
                     NIVAVTACPTGVAHTFMSAEAI"
BASE COUNT          919 a          664 c          661 g          795 t
ORIGIN      
        1 acgccatttt ttgagctaaa tcacaaaata aaatcgtttt aatctttagt ttaaatcatc
       61 aaaccttaaa ttttgtgaca tagatcacat tttgaaacat ttttatggat atttttagtt
      121 ggctataacg gtttagcaaa atatagagta aactaaccat aacattacag tttaaaaatc
      181 aactttaaat ccaaggagtt aaaatgctta atttatcggc aaaaaacatc cgtttaaatg
      241 gttcagcaag caacaaagaa gaagttatcc gttttgttgc cgccgaatta gtcgccaagg
      301 gcaatgtagc agccggctat gaagtcggta tgcttaatcg tgaaacccaa acctctactt
      361 ttttaggtaa cggaattgct attccccacg gcacgcttga tacccgtgat ttagtcaaag
      421 aaaccggcgt gcaaattatc caacttccgc aaggcgtgga atggggcaaa ggcaacactg
      481 cttatgtagt tatcgggatt gcggctaaat cggacgaaca cttaacctta ctccgtcaac
      541 tcaccactgt attaagtgat gaagaggctg ctgccacatt agcgaaaacg caaaatttag
      601 atgaatttat tgccgtatta agcggtaaga aaaaccttcc tgccttatct caagagctga
      661 tttctctgaa tatcgaatct tcaagcctta tcactttatc agccattaac gccgcaaaac
      721 tacaagagca aggctatgtt tcccaagcat tcatcagcga tgtggttgcc aacaaagcgt
      781 tacatttagg caataatcta tttgtggtgg attccgccca aggcaaccaa gctaacggta
      841 tcgctattgc tcgtaatacg caaggtcaaa ccctactaac tgtggcagaa accgacaatg
      901 ctctacagcc actattaact caattattaa aaccagaagt aagaagccag tttgctcatt
      961 tctctgcgga gcaaatgctt ggtttattaa aaggcgaaag cgtagcttca acccctgtaa
     1021 ccaatgtcga agcgagtggc aaccaagtgg ttggcacatt caccattcgt aacgacaacg
     1081 gcttacacgc ccgcccggct gcggtgttag tacaaacggt gaaaccgttc tcctcaaaaa
     1141 tcagcgttga aaatttagac cgcaacactg caccggcaag tgcgaaaagt gcaatgaaag
     1201 tagtagcact aggggcaagc caagggcatc gtttacgctt tgtggcagag ggagaagacg
     1261 cccaacaagc gattgaggca ttacaacaag caattattaa cggcttaggc gaaagtgttt
     1321 cattcgtacc ggcggtggca gatacgattg aaagcattgc tgtttctgca attcctgcgg
     1381 aagaaactgc gactgctgtc gatgccaata cgaaagaagc aacttttgta attaaaaacg
     1441 aacacggttt acacgctcgc ccaagtgcgg tgttggtgaa tgaagtgaaa aaattcaacg
     1501 ccaaaattga agtgcaaaat gttgataaaa attcaccgct tgtaagtgcc aaaagcttaa
     1561 tgaaaattgt tgcccttggc gtaaccaaag gcacgacgct acgctttgtg gcaagcggtg
     1621 atgatgccga agtagcactt gcggcaatcg gtgctgcgat tgaagcaggt ttaggcgaat
     1681 aagcgaggaa tcattatgcg tattgcaacc gttaccctca accctgcctt tgatttagtc
     1741 ggcaaacttc aacgtattga aatcggcgaa gtgaacaccg tagaaaccct tggtttatac
     1801 cctgccggca aaggtattaa cgttgctaaa gttttagctg acttaggcac aaaactttcg
     1861 gttaccggct ttttaggcga agaaaatcaa ggcgatttcg taaatgcctt taaacacaat
     1921 gatttggctg atgaatttta ccgtattaac ggtaaaaccc gcattaatgt caaaatcact
     1981 gaaaccgagg ctgatgtaac tgatctgaac tttctcggct ttgaaatttc ggaaagcgat
     2041 tgggaaaaat tcgtcacatt atctcatcag tgggaaagca aatttgactt agttgcagtg
     2101 tgtggatctc tccctcgtgg cgtaacgccg gaaaaatttg cttcttggtt ggaatcttta
     2161 caccgccaag gcttaaaagt ggtgttagat accagcaatg cggctctcac cgccggctta
     2221 accgctcacc cttggttagt aaaaccaaac cgccgagagc tggaagtgct ggtcgggcat
     2281 tcactcaact caatcgaaga gattattagt gcagctaaac aattacgttc ccgtggtatt
     2341 gaaaatgtaa ttatatcaat gggcgaacaa ggctctcttt ggctaaatca cgaaggcgtg
     2401 ttacaagccc gcccaccacg ttgcgaaaat gtagttagca ctgtaggtgc tggtgattca
     2461 atggttgccg gcttgatcta cggcttatcc caaggttgga gcaaagagca gaccttgcgt
     2521 tttgcctcag ctacttctgc tctagctgtt gttcaaagta acgttgggat tgcagaccgt
     2581 gcgacgttag acgatattat taaacaaacc gacattaccg tgatttcaga aaatcattaa
     2641 acctcaatca aaggaacaaa ccaatatgaa tatttctttt gtatttcctc aaacactagg
     2701 caacgcacgc cgtttcttag tgaatgaaat gttatctgcc gctgccaagc aacaaggaca
     2761 caatgttgtg gcggcaaacc aagctgattt tgtggtgtta ttcgataacg caattccaca
     2821 agaagctgcc ggcaagcaag gggcaatttt ggacttagaa caagcctttg ctcaacctga
     2881 agccagtctc aaacaagcgg tcgaaaatgc acaaactttt gcaaatgcta ccgcaaccac
     2941 tgcggtttca gcggtttcat ctggtgtgaa aaatatcgtt gctgttaccg cttgcccaac
     3001 tggcgtggct catactttta tgtctgcgga agcaatcga
//
LOCUS       DANKBV010000192         3029 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.192, whole genome shotgun sequence.
ACCESSION   DANKBV010000192 DANKBV010000000
VERSION     DANKBV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.192"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(3..956)
                     /gene="corA"
                     /locus_tag="PWY43_002355"
     CDS             complement(3..956)
                     /gene="corA"
                     /locus_tag="PWY43_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDL2137185.1"
                     /translation="MIRAFGLDNACLISVDETNPTLLNDAIWIDLIDPSEAERTVLEN
                     RLDQTLAEERELEDLEASARFFEDEDGLHLHSFFYCLDDDDYADIATVAFTIRDGRLF
                     TLRDRDLPAFRLYRMRSRREKLIDSNAYELLLDLFETKIEQLADVIEEVYADLEKLSR
                     VILNGQQENENFNDALSRLTEFEDASSKVRLCLMDTQRALSFLLRKTRLPNNQLEQAR
                     DIMRDIESLQPHNESLFQKVNFLMQAAMGYINIEQNRIMKFFSVVSVMFLPATLVAST
                     YGMNFEFMPELHFQYGYPMAIGLMIVAASAPYLYFKRKGWL"
     gene            1253..2041
                     /gene="ubiE"
                     /locus_tag="PWY43_002356"
     CDS             1253..2041
                     /gene="ubiE"
                     /locus_tag="PWY43_002356"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDL2137186.1"
                     /translation="MSDVEQLAPKDEVQQSAETTHFGFKTVAKEEKQQLVANVFHSVA
                     GKYDLMNDLLSFGIHRVWKRFTIDCSGVRKGQKVLDLAGGTGDFTAKFSRIVGESGEV
                     ILADINSSMLEVGREKLRNLGVVGNVSYVQANAECLPFADNTFDCIVISFGLRNVTDK
                     DKALRSMFRVLKPGGRLLVLEFSKPIIDPISQLYNFYSFNILPKVGEVVVNDAESYRY
                     LAESIRMHPKQDELKAMMEQTGFESVNYYNLSAGIVALHRGYKF"
     gene            2232..2879
                     /locus_tag="PWY43_002357"
     CDS             2232..2879
                     /locus_tag="PWY43_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HDL2137187.1"
                     /translation="MLNNVKQQLMLPQFTFGLIETAFNALLKRSPHIEPNLRKLSGKV
                     LKIALTSPKIDFFVLFSESRTDWLGNYEGEADCTVQLAFDTLPKLADKQKLTELINNK
                     SLVLTGDIQVLQHFTALLEQLEKDPAELLSPFVGDVVAQTSTDLAKKLLGKVKAQFEQ
                     NNQHLVENLIQERPVLVHRLQAVHFYDQVEELAQQALKLEQKFTHYVRSSSHQQS"
BASE COUNT          955 a          595 c          653 g          826 t
ORIGIN      
        1 aattataacc aacctttacg tttaaaataa agataagggg cggaggcggc aacaatcatt
       61 aaaccaatcg ccatagggta gccatattgg aaatgaagtt ccggcataaa ttcaaagttc
      121 ataccgtaag tagaagcaac cagcgtagca ggtaggaaca tcactgacac caccgagaag
      181 aatttcataa ttcggttctg ctcaatattg atataaccca ttgccgcttg cattaaaaaa
      241 ttcacttttt ggaacaagga ttcattgtgc ggttgcagag attcaatatc tcgcataatg
      301 tcacgggctt gttctagctg attattcggc aaacgggttt tacgcagtaa gaaacttaac
      361 gcccgttgcg tatccatcaa acataaacgc acttttgaac ttgcatcttc aaattcagtt
      421 aagcgagaaa gggcatcatt gaaattctca ttttcttgct gaccgtttag aataacccga
      481 cttaattttt ctaagtcagc atagacttct tcaattacgt ctgcaagctg ctcgattttg
      541 gtctcaaata aatcgagtaa gagttcatag gcgttgctgt caatcaattt ctcacggcgg
      601 gaacgcattc gatacaagcg aaatgcgggt aaatcccgat cacgcaaggt aaacaaacgc
      661 ccgtcacgaa tagtgaaggc gacagtggca atatcggcgt aatcgtcatc atctaagcag
      721 taaaagaacg agtgcaggtg caaaccgtct tcatcttcaa agaaacgggc agacgcctct
      781 aagtcttcca attcacgttc ttcagcgagt gtttggtcta agcggttttc taatacggtt
      841 cgttcagctt ctgatggatc gatgagatca atccaaatcg catcatttaa taaagttggg
      901 ttagtttcat caacgctgat taaacatgcg ttatctagtc caaaagcacg gatcatgttt
      961 agctccaaat agttaggagg cttggaacaa aaaggaacaa agatactagg cgagcattac
     1021 cgaccttgtc ggtaacattg aggttagaca agataggtta aacgggtaag tatcgactat
     1081 gactgtccaa aatgtgtaat cctcaagaaa aaatagtgtg cgaatgttac agaatttggc
     1141 ttttctagtc aagaaagcgg ttatttactt aagaaaattt gcaaaaaatt aaagaaaaac
     1201 gaccgctttt ttgctatggt tacgcctaaa ttttgcaact agagacaaga aaatgagcga
     1261 tgtagaacaa ttagcaccta aagatgaagt gcaacaatcg gcagaaacca cccactttgg
     1321 gtttaaaacc gtagcgaaag aagaaaaaca gcaactggtc gcaaatgtgt ttcactcggt
     1381 ggcaggcaaa tatgacctaa tgaacgattt gctttctttc ggcattcatc gagtgtggaa
     1441 acgctttacc attgattgca gtggcgtgcg taaagggcaa aaagtgctgg atcttgccgg
     1501 cggcacgggt gattttaccg caaaattctc tcgcattgtt ggtgaaagtg gtgaggtgat
     1561 tttagccgat attaacagct caatgttgga agtcgggcgg gaaaaattac gcaatttagg
     1621 cgtggttgga aatgtgagct acgttcaagc gaatgccgaa tgcttgcctt ttgctgataa
     1681 cacttttgat tgtattgtga ttagttttgg cttgcgtaat gttaccgaca aagacaaagc
     1741 cctacgttca atgttccgcg tgctaaaacc gggcggtcgc ttacttgtgt tggaattctc
     1801 aaaaccgatt atcgacccaa tcagccagct ttataatttc tactcattca atattttgcc
     1861 gaaagtgggc gaagtggtgg taaatgacgc cgaaagttac cgctatttag ctgaatcgat
     1921 ccgaatgcac ccgaaacaag acgagctaaa agcaatgatg gaacaaactg gctttgaaag
     1981 cgtgaattac tacaacttaa gtgccggcat tgtggcatta cacagaggtt ataaatttta
     2041 gttttctctt aaatgactct ctccctttgt gggagagaga ccgcttatga gcgtttagcg
     2101 aaataagcag agagaggggg caagcggtca tatttattca atattttgca aatacccctc
     2161 tccctgattt tgcttcttaa tgaagaacaa tctatccttc tcccaaaagt gaagagggta
     2221 agtaggtacg aatgttaaat aatgtaaaac aacaattaat gctcccgcaa tttacctttg
     2281 gtctgattga aaccgctttt aatgcgttac ttaaacgctc gcctcatatt gaaccgaatt
     2341 tgcgtaaatt gtccggaaaa gtgctaaaaa tcgccctaac ttcgcctaaa atcgactttt
     2401 ttgtgttgtt tagcgaaagc cgaaccgatt ggcttggcaa ctacgagggg gaagcagatt
     2461 gtacggtgca actggcattt gacaccctgc cgaaactagc ggataagcaa aaattaaccg
     2521 agctgattaa taataaatcg ttggtgctga cgggcgatat tcaagtgttg caacatttca
     2581 ctgcattact tgaacagctc gaaaaagacc ctgccgaact gctttcccct tttgttggcg
     2641 atgtagtggc tcaaactagc accgatcttg ctaaaaaatt gctaggtaaa gtaaaagctc
     2701 aatttgagca aaataatcaa catttggttg agaatttaat ccaagaaaga ccggtgttgg
     2761 tgcatcgttt acaggcggtg catttctatg accaagtcga agaacttgcc caacaagcgc
     2821 tcaaattaga gcaaaaattt actcactacg ttcgctcttc gagccatcag caaagctgat
     2881 gttcaaacgc aagcgtttgt gcaaaattaa atttaacaaa ataaaattgt gatatgactc
     2941 gaaaagttga gtgaaagaaa ttgcctccgg atagattaag cataaactta atccaaaacg
     3001 gaggctttta tgttaataga aacaacgat
//
LOCUS       DANKBV010000193         2920 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.193, whole genome shotgun sequence.
ACCESSION   DANKBV010000193 DANKBV010000000
VERSION     DANKBV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.193"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            230..826
                     /gene="grpE"
                     /locus_tag="PWY43_002358"
     CDS             230..826
                     /gene="grpE"
                     /locus_tag="PWY43_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL2137188.1"
                     /translation="MTTQNEHLQEEVKQEEIQPEVVTEETQAEEQNTPQEEPLALAQA
                     RIAELEAYIAEADKREQDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVD
                     NLERGLDALDKAVTDETTQALVDGVEMTHKEFINTLAKFGVVAIGEVGEAFNPELHEA
                     ISMQPAEGIEANHVSTVLQKGYTLHGRVIRPAMVMVAN"
     gene            924..1193
                     /locus_tag="PWY43_002359"
     CDS             924..1193
                     /locus_tag="PWY43_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137189.1"
                     /translation="MDAAMSLRFDKLRFVKRLQEANQTPEMAEAFANALDEALEQSQS
                     PLATKADLQLELEKLKNEINTTIFKAITLNITILGFLMAIMKFIN"
     gene            1256..1708
                     /locus_tag="PWY43_002360"
     CDS             1256..1708
                     /locus_tag="PWY43_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL2137190.1"
                     /translation="MTLQFNAVALLLVVLIILGLISQSSAVTISATVLLIMQQTLLSK
                     FIPFVDQYGLKIGIIILTIGVLSPLVSGRITLPELAQLLNWKMALSIIAGVLVAWLGG
                     RGVNLMGSQPVLVTGLLIGTVIGVAFLKGVPVGPLIAAGILSLIVGKT"
     gene            1812..>2920
                     /locus_tag="PWY43_002361"
     CDS             1812..>2920
                     /locus_tag="PWY43_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2137191.1"
                     /translation="METPLQKNHKKPPLVKLENITTADFRRLLGRLKGAENIKNIDHK
                     RSVLAEISQDLTACQARFFARTQHAQNLKIDYPDLPVSARREEILKLISENQVVVIAG
                     ETGSGKTTQLPKMCLELGRGVKGLIGHTQPRRIAARSVATRIAEELKSELGETVGYKV
                     RFNDQVSENSLIKLMTDGILLAEIQHDRYLNQYDTLIIDEAHERSLNNDFILGYLKQI
                     LHKRPDLKVIITSATIDVERFSKHFNNAPIIEVSGRTFPVEVRYRPIVEDEDQLQGIL
                     NAVDELQAEGRGDILIFMNGEREIRDTAEALQKQELRHSEILPLYARLSAAEQQRIFQ
                     PSGGLNRIILATNVAETSLTIPNIKYVIDTGTARIS"
BASE COUNT          895 a          631 c          644 g          750 t
ORIGIN      
        1 tttttcattt aagttttctc cgttaaagaa aagtgattaa aaaatatagc cttacctaag
       61 ggcggaaaag actagggcat tttaagcccc cccccgtatt ttgcaagcct tttctgatta
      121 aaaagtgaat tattttcgca aaaagaaaga gatatgcttg aaatccctta atttatcgct
      181 atattaagga agatttacat ttccagatta gtcttttaaa ggataaaaaa tgaccactca
      241 aaatgaacac ttacaagaag aagtaaaaca agaagaaatt caacctgaag ttgtaactga
      301 agaaactcaa gcggaagaac aaaatacacc tcaagaagaa ccgttagctc tcgctcaagc
      361 tcgaattgcc gagttagaag cctatattgc agaagccgac aaacgtgagc aagatattca
      421 gcttagggct caagcagaaa ttcaaaatat ccgccgccgt gcagagcaag atgttgaaaa
      481 agctcacaaa ttcgctttag aaaaattctc caaagaattg ttaacagtgg ttgataacct
      541 tgaacgtgga ttagatgccc ttgataaagc cgtgaccgat gaaaccactc aagcgttagt
      601 tgacggcgtt gaaatgactc ataaagaatt catcaataca ctggctaaat ttggtgtggt
      661 ggcaattggc gaagtaggcg aggcatttaa ccctgaattg cacgaagcta tttcaatgca
      721 acctgccgaa ggaattgaag ccaaccatgt cagcacagtg ctacaaaaag gctacacgct
      781 tcacggacga gtgattcgcc cggcaatggt gatggtggca aactaaattg tcgattttcg
      841 acgaagatca caaaatctcc taaaattcct gcttgtgatt atgctaaatt agtttataac
      901 ttaaccacaa gaggataatt gaaatggatg cagctatgag cctacgattt gacaaattac
      961 gttttgtaaa acgcttgcaa gaagccaatc aaacacctga aatggctgag gcatttgcta
     1021 atgctctcga tgaagcctta gagcaaagtc aaagcccact agcaacaaaa gctgatttgc
     1081 aacttgaact tgaaaagcta aaaaatgaaa tcaataccac gatatttaaa gcaattacct
     1141 taaatatcac aattcttggc tttttaatgg caataatgaa atttattaac tagctcaacg
     1201 tttaattaac cactccctta cttctatgaa gtaagggttt ttacgtttta atcctatgac
     1261 tttacaattt aacgcagtgg cacttctact tgtggtgctg attattttag ggctgatcag
     1321 ccaaagcagt gcggttacta tttcagccac cgtattgctg attatgcaac aaaccttact
     1381 ttctaaattt atcccctttg tcgatcaata cggcttaaaa atcgggatta tcattttaac
     1441 catcggcgta cttagcccct tggtttcagg gcgaattacg cttcccgaac ttgcccaact
     1501 attgaactgg aaaatggcac tctcaataat tgctggcgtg ctggtagcgt ggctaggtgg
     1561 caggggcgtg aatttaatgg gcagccagcc ggtgttggta actgggcttt taatcggcac
     1621 ggtgatcggc gtggcgttct taaaaggcgt accggtcggg ccgttgattg cggcagggat
     1681 tttgtcactg attgttggta aaacctaatg tagggtggac gtaagtccac gcataaaatc
     1741 cctattctgt tacgtgggct tacgcccacc ctacaataaa tcacaaggtc ttaatgcaaa
     1801 agagaaatta aatggaaaca cctttgcaaa aaaatcataa gaaaccaccg cttgtgaagc
     1861 tggaaaatat caccaccgcc gatttccgcc gcctgctcgg tcgcttaaaa ggggcggaaa
     1921 atattaaaaa catcgatcac aagcggtcag ttttggcaga aatttcgcaa gatctcaccg
     1981 cttgtcaggc acgttttttc gcacgaactc agcacgccca aaacctgaaa attgactacc
     2041 ctgatctgcc tgtttccgct cgccgtgagg aaattttaaa actgattagc gaaaatcagg
     2101 tggtggtgat cgcaggggaa accggttcgg gcaaaaccac ccagttaccg aaaatgtgct
     2161 tagaactcgg gcgtggggtg aagggcttga ttgggcatac ccagccccgc cgaattgccg
     2221 cacgttcggt ggcgactcgc attgccgaag agctgaaatc ggaactgggc gagacggtcg
     2281 gttacaaagt ccgctttaac gatcaagtca gtgaaaacag cttaatcaag ctaatgacgg
     2341 acggtattct gctggcggaa atccagcacg accgctattt aaatcagtac gatacgctga
     2401 ttattgacga agctcacgag cgttcgctga acaacgattt tattttgggt tatctgaaac
     2461 aaattctgca caaacgcccc gatctgaaag tgatcatcac ctcggcaact attgatgtag
     2521 aacgtttttc caaacatttc aacaacgccc cgattattga agtctctggt cggacattcc
     2581 cggtggaagt gcgttatcgc ccgatagtgg aagatgaaga tcaactgcaa ggtattctaa
     2641 atgcggtgga cgaactgcaa gccgaagggc gtggcgatat tctgattttt atgaacggtg
     2701 aacgggaaat tcgtgacact gccgaagcct tgcaaaagca ggaactgcga catagtgaaa
     2761 ttttaccgct ttacgcccgc ttgtcggctg ccgaacagca acggattttc cagccaagcg
     2821 gtgggctaaa ccgcattatt ctggcgacca acgtggcgga aacctcgctc actattccga
     2881 atattaaata tgtgatcgac accggcacgg ctcgtatttc
//
LOCUS       DANKBV010000194         2904 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.194, whole genome shotgun sequence.
ACCESSION   DANKBV010000194 DANKBV010000000
VERSION     DANKBV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.194"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            24..347
                     /locus_tag="PWY43_002362"
     CDS             24..347
                     /locus_tag="PWY43_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2137192.1"
                     /translation="MKLSSELQNQINQLNQLRKQALKQEQDEQKLFEKNKIEDLPHFG
                     TQLNAQRKLLGIELTTLEMQTGISSSTLKRLFKDPSQVKFQTICIVAETLGFNLCAIN
                     THKQE"
     gene            complement(424..1443)
                     /gene="glpX"
                     /locus_tag="PWY43_002363"
     CDS             complement(424..1443)
                     /gene="glpX"
                     /locus_tag="PWY43_002363"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDL2137193.1"
                     /translation="MKRTLSFEFSRVTEAAALAAYSWLGRGDKNAADEAAVKAMRFML
                     NQMEIRGEVVIGEGEIDEAPMLYIGEKIGLSRLEDEEISIAVDPIDGTRMTAMGQANA
                     LSVLAAGGKETFLKAPDMYMEKLVVGQECKGMIDLNLPLEQNLRRVASKKGKLLSQLT
                     IAILAKPRHEKIIADVQKLGVRVIAIPDGDVATAVQCCLPESELDLLYGIGGAPEGVV
                     AGAAVRALGGDMQARLIPRNQVKGNSPENLTATEKELSRCNEMNVPVNTVLKLEDLVR
                     DDNIVFVATGITSGELLKGIKRRGNIASTETLLIRGKSRTIRKIQSDHYVDRKDNELL
                     SLLDL"
     gene            1640..1858
                     /gene="zapB"
                     /locus_tag="PWY43_002364"
     CDS             1640..1858
                     /gene="zapB"
                     /locus_tag="PWY43_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HDL2137194.1"
                     /translation="MSLSVLDQLEEKIKQAVETIQLLQLEVEELKGKNDAAKQENDTL
                     RSEYEQLKVEQQNFQDRLRSLLGQIDNV"
     gene            1914..2495
                     /locus_tag="PWY43_002365"
     CDS             1914..2495
                     /locus_tag="PWY43_002365"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2137195.1"
                     /translation="MAKLYFYYSSMNAGKSTTLLQSSYNYQERGMNTLVYTAAIDDRF
                     GVGKVNSRIGISQEANLFTADTNLFAEIKALHSNKALHCILIDEAQFLTKTQVYQLSD
                     VVDKLKIPVLCYGLRTDFQAELFEGSKYLLAWADELEELKTICDCGKKAHFVVRLNEK
                     GEAIKDGEQIQIGGNDRYLSVCRYHYKQKLGKL"
     gene            complement(2586..>2904)
                     /locus_tag="PWY43_002366"
     CDS             complement(2586..>2904)
                     /locus_tag="PWY43_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HDL2137196.1"
                     /translation="GDVYSCDHFVYPDYHIGNLNQQQLAEIVSSAAQRQFGQNKQAKL
                     SQACLNCKFRILCHGGCPKHRIVPQANSENHNYLCPSYQQFFAETSSVMRQMSEQIRF
                     AGK"
BASE COUNT          900 a          608 c          555 g          841 t
ORIGIN      
        1 tctactttta gctcaaaaat aaaatgaaac tatcaagcga actacaaaat cagataaacc
       61 aactaaatca gttacgaaaa caggcactca aacaagagca agacgagcag aaattgtttg
      121 aaaaaaataa aattgaagat ttgccacatt ttggcacgca gcttaatgct cagaggaaac
      181 tattaggcat agaattaacc acccttgaaa tgcaaaccgg catttcaagc tctaccctaa
      241 aacgcttatt taaagaccca agccaagtga aatttcagac aatttgtatt gtggcagaaa
      301 cactcggctt taatttatgt gcaattaata ctcacaaaca agagtaagcg tgacaatgta
      361 gtgattttga ttactttggg aagacaaaaa atcgggctaa aagcccgatt tatcagagaa
      421 aagctataaa tccaataaag aaaggagttc gttatcttta cgatcgacat aatggtcgga
      481 ttgaatttta cgaatggttc tagatttgcc acgaataagt aaggtttcag tgctggcaat
      541 attgcctcta cgtttaatcc cttttaacaa ttcacctgaa gtaatgccgg ttgcaacaaa
      601 gacgatattg tcgtcacgca ccaaatcttc taatttcaac accgtattca ccggcacatt
      661 catttcatta cagcgtgaaa gctctttttc ggtagcagtt aaattttccg ggctattccc
      721 cttcacttgg ttgcgaggaa ttaaacgtgc ttgcatatcg ccacccaatg cacgaaccgc
      781 agcccccgcc actacccctt caggtgctcc gccgatacca taaagcagat ccaattcgct
      841 ctctggcaaa cagcattgca ccgcagtcgc aacatcgcca tcaggaatcg caatcactct
      901 tacccctaat ttttgcacat cggcaattat tttttcgtgg cgaggcttag caagaatagc
      961 aatggttaat tgggaaagta atttgccctt tttcgaggcg acacggcgaa gattttgttc
     1021 aagcggaaga tttaaatcaa tcattccttt gcattcctgc cccaccacta atttttccat
     1081 atacatatca ggggctttta aaaaggtttc tttgccgcct gctgctaata ccgacaacgc
     1141 atttgcctgc cccattgcag tcatacgagt accgtcaatc gggtcaaccg caatactgat
     1201 ttcctcatct tctaggcggg atagaccgat tttctcgcca atataaagca tcggtgcttc
     1261 gtcaatttcg ccctcaccaa ttaccacttc gccacggatt tccatttggt tcagcataaa
     1321 ccgcattgct ttcaccgcag cttcatcagc ggcattttta tccccacggc ctagccaaga
     1381 gtaagctgct agagccgctg cttccgtaac acgagaaaat tcaaaagaaa gagttcgttt
     1441 catttttgtt ccttagtttc ttgaaagaaa aactttaaaa gaagagagat tatattttat
     1501 cagtaacctg caagctacgt tactaaaaaa tcagcgtttg aataaaaatt cacaaatagc
     1561 caaacttttt tataaaaata aagtcagatt tagagtataa tctttacaag tttttatatt
     1621 attttagagg gaatttacta tgtcattatc ggttttagac caattagaag aaaaaattaa
     1681 acaagctgtt gaaactattc aattacttca attagaagtt gaagaattaa aaggtaaaaa
     1741 cgatgcagcg aaacaagaaa acgacacctt gcgcagcgaa tatgaacaat taaaagtgga
     1801 acaacaaaac ttccaagatc gtttacgttc acttttaggt caaatcgaca acgtttaatt
     1861 ctattttcag cacaaacggc taaattttta gccgtttttt atttgttaca gctatggcaa
     1921 aactctattt ttactactcc tcaatgaatg caggcaaatc tactactctg cttcaatctt
     1981 cttataatta ccaagaacgt ggaatgaaca ctttagttta taccgccgct attgatgacc
     2041 gtttcggtgt ggggaaagtc aactctcgta tcggtatttc acaagaagcc aatttattta
     2101 ccgctgatac caatctattt gcggaaatta aggcattaca ctcaaacaaa gcgttacact
     2161 gtatcttgat tgatgaggct cagtttttaa ctaaaacaca ggtttatcaa ttaagtgatg
     2221 tggtcgataa actcaaaatt ccggtgcttt gctacgggct acgcaccgat ttccaagctg
     2281 aattatttga aggtagcaaa tatttgttgg cttgggcaga tgagctggaa gaactcaaaa
     2341 ccatctgtga ttgtggcaaa aaagcccatt tcgtcgttcg attaaatgaa aaaggcgaag
     2401 ccattaaaga cggtgaacaa atacaaatcg gcggcaatga tagatatctc tctgtttgcc
     2461 gttatcatta caaacaaaag ttaggtaagc tataagctac aagaagcagc aagcggttaa
     2521 attttcggga atttttgcaa attttcagcg aaatttaacc gcttttttgt gttatttacc
     2581 tctctttact ttcctgcaaa ccgaatttgc tcactcattt gtctcatcac actcgaagtc
     2641 tcggcaaaaa actgttggta agaggggcaa aggtagttgt ggttttcgct gtttgcttgg
     2701 gggacgatgc ggtgtttggg gcagccgccg tggcaaagta tgcggaattt gcagtttagg
     2761 caggcttgcg agagttttgc ctgtttattc tgcccgaatt ggcgttgggc ggctgatgag
     2821 acgatctctg ccaactgctg ttggttgagg ttgccgatgt ggtaatcggg atagacgaaa
     2881 tggtcgcaac tgtacacatc gcca
//
LOCUS       DANKBV010000195         2865 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.195, whole genome shotgun sequence.
ACCESSION   DANKBV010000195 DANKBV010000000
VERSION     DANKBV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.195"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(512..1024)
                     /locus_tag="PWY43_002367"
     CDS             complement(512..1024)
                     /locus_tag="PWY43_002367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137197.1"
                     /translation="MGKINYFDDIKIGNEIFSLSHLNPKYLEFYGQKVQKTLKLKVLY
                     SDHCFTARVNRLQSDSERVFSIERYNLSKLLPNIIEGLNNDKIQVFQTLARRNFAYVV
                     KTTLNDKDYNIFFEVKKSNNSNPDCDLVIRIESAYVFDNDCKLEYSGKIRFLILCQKI
                     YQGEKIQCRK"
     gene            complement(1024..1242)
                     /locus_tag="PWY43_002368"
     CDS             complement(1024..1242)
                     /locus_tag="PWY43_002368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137198.1"
                     /translation="MATIVQKDVLIEAIAQVQGYLLRSLPSSDSMNDDELFLCELREK
                     IYNTHHDKLDYESLLADIVKIKNKSCYS"
     gene            complement(1253..1450)
                     /locus_tag="PWY43_002369"
     CDS             complement(1253..1450)
                     /locus_tag="PWY43_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HDL2137199.1"
                     /translation="MSNEELTPEVLARRAYHVRNALASFSLEGEYPSKEAEDLFNKFA
                     SGEIETIDELRVQINLLYSED"
     gene            complement(1458..1664)
                     /locus_tag="PWY43_002370"
     CDS             complement(1458..1664)
                     /locus_tag="PWY43_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137200.1"
                     /translation="MATLMEKDSLINGISQTLGLIVAQIGDRNEDSVMLATLRNKLYG
                     LDAEKIDYQGTAKLVKEKFNLYKL"
     gene            complement(1674..2651)
                     /locus_tag="PWY43_002371"
     CDS             complement(1674..2651)
                     /locus_tag="PWY43_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2137201.1"
                     /translation="MANDLVVVKANSLIEASYRLSIDEIRILALTIGTMDPKSNQQIF
                     DFTVADFVREFPEISMDNAYKQIQAAIKRIYERSVRTEDSNRVTEFRWVSSRTYFKNE
                     GRFRIAMTNEVMPYLTQLKGQFTQYQLRHISSFNSVHSIRIYELVTQYRSVGYREITI
                     EDLKKWLQVEDKYPRFNNFKQWVLEPSITEINDKSDLLVDVEQIKRGRSIIALKFTIK
                     SKKSAVKAELKRPPFPHKNKYGKFVTLNRQDPRMSNHEYGLWAKDCLKILEGFYQKIE
                     DIPNEDLLFYWIFLTGNASNKSKLGTRKNFVDELKKRGYKIEHCELVKV"
BASE COUNT          931 a          526 c          414 g          994 t
ORIGIN      
        1 aaaaaaaaca aacctaaaac aagtaaaaaa ctaaaaaact aaaatataga gcctatcggc
       61 tcttttcggg ctttcagccc tattttttta tcttttttac actagaaaaa cctttttaaa
      121 ctttggtaat accaaataca acctaataat actaaataaa acctttttta tacctaaata
      181 ctacgttgtg tatattaaaa gtttacttaa atgagtgggc aagatatgta aactagacct
      241 actttttgca aagtatatct agtttactcg gataaattcg ctacagctca aatcctatat
      301 cttgtccttc ggagttttta caccaggagt gatgaatagg catcaatagc aaacttgaca
      361 tattgagaaa agacgccata atactaatac aacctatggc aacccgatga aatcgatcat
      421 aggacggctc agaaatgagt aaggtgcgat gccttggtaa gtgtattcgg ggtctattta
      481 agagaaaagc cacgttttaa cgtggctttt ctcattttcg gcattgtatt ttttcacctt
      541 gataaatttt ttggcaaagt atcaaaaatc gaatctttcc tgaatattcc agtttacaat
      601 cattatcaaa tacataagca ctttctatac gaataactag atcacaatct ggattgctgt
      661 tgttactttt tttgacctca aaaaatatat tgtaatcttt atcgtttagc gtagttttga
      721 ctacgtatgc aaaattacgt ctagctaacg tctgaaaaac ttgaattttg tcgttattta
      781 gcccttctat gatgtttgga agtaattttg ataggttata tctctctata gagaaaacac
      841 gctcagaatc gctctgtaag cgatttacgc gagcagtaaa acagtgatcg gaatacagaa
      901 cctttaattt aagcgttttt tgtacttttt gaccgtaaaa ttcaagatat ttaggattga
      961 gatgagagag tgagaaaatc tcattaccaa tttttatatc atcaaagtaa tttatctttc
     1021 ccattaacta taacaactct tatttttaat ttttacaata tcagcaagta atgattcata
     1081 atctagttta tcgtgatgag tgttataaat tttctcacgt agctcacaca aaaatagttc
     1141 atcatcattc atggagtcag aggagggtaa gctacgtaat aaataacctt gaacctgggc
     1201 aatcgcctca attaaaacat ctttttgaac tatagttgcc atttttacct tcctaatctt
     1261 cagaatacaa taaattaatt tgaactcgca attcatctat tgtttcaatt tcaccagaag
     1321 caaatttatt aaatagatcc tccgcctctt tagagggata ttccccttcc aaagagaaac
     1381 tagccagggc atttcttaca tggtatgccc gtcgagctaa aacttctggt gttaattctt
     1441 cattactcat aaaactccta tagcttatag agattaaatt tttcttttac aagttttgca
     1501 gttccttgat aatcaatttt ctctgcatca agtccatata acttattacg caatgttgct
     1561 aacataacac tatcctcgtt tctgtcgcct atttgagcta cgattaaacc tagtgtttga
     1621 gaaataccat ttatcaagct gtctttttcc ataagtgttg ccatattacc ctcttaaact
     1681 ttaaccaatt cacaatgttc tatcttgtat cctcgttttt taagttcatc aacaaagttt
     1741 tttcttgttc ctaattttga tttattgctt gcattacctg ttaggaaaat ccaataaaac
     1801 agcaaatctt cattcggtat atcttcgatt ttttgataaa aaccttccaa tatttttaaa
     1861 cagtcttttg cccataaacc atactcatga ttgctcattc ttggatcttg tcggtttaac
     1921 gttacaaact taccgtactt attcttatgt gggaatggtg gtcgttttaa ttctgctttg
     1981 accgcacttt tcttactctt aatcgtaaat tttaatgcaa taatactacg accacgttta
     2041 atttgttcta catcaacgag cagatcagat ttatcgttaa tttccgttat agatggctct
     2101 aaaacccatt gtttgaaatt gttaaaccta gggtatttat cttccacctg taaccatttt
     2161 ttcaaatctt caatagtgat ttcacgataa ccgacagagc ggtattgtgt aacaagctca
     2221 taaatacgga tagagtgaac actattaaaa cttgaaatgt gcctaagttg gtactgcgtg
     2281 aattgtcctt ttaactgcgt aaggtatggc attacttcgt tagtcatagc aatacgaaaa
     2341 cgcccttcat ttttgaaata agtgcgagaa gatacccagc gaaattcagt tacacgatta
     2401 ctatcttcgg ttctaacact acgttcataa atccgtttga tagccgcctg aatttgctta
     2461 taagcgttat ccatactgat ctcaggaaat tcacggacaa aatcggctac cgtaaaatca
     2521 aaaatttgct gatttgattt tggatccatt gttccaatcg ttaaagctag aatacggatt
     2581 tcatcaatac ttaatcggta actagcttca ataagactat ttgctttcac tacaactaaa
     2641 tcatttgcca taagactaga tatttcctgt taaaaaataa cctttaaaac tagataaata
     2701 ataacaccta gctaattaaa aagactagat aaattttaac atcaagtcga caacaggaaa
     2761 tttctagtcg tataacagga aatttctagt cgtataacag gaaatttcta gtcgtataac
     2821 aggaaatttc tagtcgtata agtttctaac ccattgtaat aaaag
//
LOCUS       DANKBV010000196         2836 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.196, whole genome shotgun sequence.
ACCESSION   DANKBV010000196 DANKBV010000000
VERSION     DANKBV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.196"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1048)
                     /locus_tag="PWY43_002372"
     CDS             complement(<1..1048)
                     /locus_tag="PWY43_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HDL2137202.1"
                     /translation="MRTIAIITSPNNADFIQQGYFYIPENHQLKAIPFSNAKTILGQE
                     YPFAIYTMQAENGVNFNLDAFAILAGTIQENGTLFLLCPQWDNLENELDFDALRWNEN
                     QAIACPNFYQHFKQLVAKFGFEVRTNLPKLPATSGQIPSKIYQLTQEQQNILQNLPLD
                     PADIHLITAPRGRGKSTLAGRLAKALPAQNKVLITARSYSALPSFWRSAEQKIPFFAP
                     DNLIEQVKSKNISPNQWLFIDEAASLPLPILHTLCAYFEKVVLTTTTQNYEGTGRGFE
                     LKFPVMLDKPFKHWTLSQPLRWSENDPLEAFVTELLLLSEEKSPLFSKEGKGEIYSLR
                     SHYVPLLKQRSNAKR"
     gene            complement(1126..1608)
                     /gene="ybeY"
                     /locus_tag="PWY43_002373"
     CDS             complement(1126..1608)
                     /gene="ybeY"
                     /locus_tag="PWY43_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL2137203.1"
                     /translation="MENLYIDLQIACENTENLPSEQQFYTWVQKALAVEAKTDDFPES
                     EITIRIVDEAESHELNLTYRGKDKPTNVLSFPFEAPEGIEMPLLGDLVICRQVMEKEA
                     EEQGISAESHWAHLAIHGTLHLLGYDHIEEDEAVEMESLETEIMQSLGYEDPYLSEKI
                     "
     gene            complement(1626..2081)
                     /gene="moaE"
                     /locus_tag="PWY43_002374"
     CDS             complement(1626..2081)
                     /gene="moaE"
                     /locus_tag="PWY43_002374"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDL2137204.1"
                     /translation="MFETLIEVQEAEFDQNSIYQWLSEQHSVGAATLFIGKVREMNLG
                     DNVSGLFLEHYPAMTKKALQEIVAQARTRWDLQRVAVIHRIGQLHTGDEIVLVGVSSA
                     HRGDAYHANEFIMDYLKTKAPFWKREQTQAGERWIEGRESDQQAAKKWQ"
     gene            complement(2083..2328)
                     /gene="moaD"
                     /locus_tag="PWY43_002375"
     CDS             complement(2083..2328)
                     /gene="moaD"
                     /locus_tag="PWY43_002375"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDL2137205.1"
                     /translation="MIKILFFAQVRELVATDRLDVEPTFTTAEALREHLSQQGKKWQL
                     ALEKGKLLVAINQTICPLETEIKDGDEVAFFPPVTGG"
     gene            complement(2321..2797)
                     /gene="moaC"
                     /locus_tag="PWY43_002376"
     CDS             complement(2321..2797)
                     /gene="moaC"
                     /locus_tag="PWY43_002376"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDL2137206.1"
                     /translation="MTTFTHINQNGEANMVDVSMKQETVRIARAEAFVRMNAETLQMI
                     VSGNHHKGDVFATARIAGIQAAKRTWELIPLCHPLLLSKVEVQLEALPESNQVRIESL
                     CKLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMIIENVRLLSKSGGKSGDFKVD"
BASE COUNT          731 a          615 c          603 g          887 t
ORIGIN      
        1 aacgctttgc gtttgaacgt tgctttagca acggcacata gtgtgagcga agcgaataaa
       61 tctccccctt cccctctttg ctaaagaggg gagatttttc ttcggaaagt aatagcaact
      121 cagtcacaaa tgcttcgaga ggatcatttt cgctccaccg caatggttga gataacgtcc
      181 aatgtttaaa cggtttatcc aacattaccg ggaatttcag ctcaaatcct cgccccgtgc
      241 cttcgtaatt ttgggttgtt gtggttaaaa ccactttttc aaaataggca caaagtgtgt
      301 ggagtatcgg caagggtagg ctagctgcct catcaataaa gagccattgg ttaggtgaaa
      361 tgtttttact tttaacttgc tcgattaaat tgtcaggtgc gaaaaaaggg attttttgct
      421 ctgccgaacg ccaaaaactg ggcaatgcag aataagaccg tgcggtaatt aataccttat
      481 tttgtgcggg taacgctttt gcaagtcgcc ctgcaagggt agatttgccc cgcccacgag
      541 gggcggtaat caaatgaata tcggcaggat caagcggtag attttgcaaa atattttgct
      601 gttcttgcgt taattggtaa atttttgaag gaatttgacc gcttgtagcc ggtaattttg
      661 gcaaattagt tctgacttca aaaccaaatt tagcgactaa ttgtttaaaa tgttgataga
      721 aattcgggca ggcaatagcc tgattttcgt tccaacgtaa cgcatcgaaa tctaattcat
      781 tttccaaatt atcccactga ggacaaagca aaaataaagt accgttttct tgaatagtgc
      841 cggcaagtat tgcaaaagca tctaagttaa aattcacccc attttctgct tgcatagtat
      901 aaatggcaaa agggtattct tgtcctaaaa tcgttttggc attggaaaag gggatagctt
      961 taagctgatg attttcggga atgtaaaaat agccttgttg aataaagtcg gcattgttag
     1021 gagatgttat gattgcgatc gttctcatat aacttgtagg aacagctcct gtgcctgtcc
     1081 gattataatg gagtactgga gggcacagga ccctccccta caagactata ttttttctga
     1141 aagatacgga tcttcatacc ctaaactttg cataatttcg gtttcgaggc tttccatttc
     1201 cactgcttca tcttcctcaa tatggtcgta gccgagcaga tgaagtgtgc cgtgaatcgc
     1261 caaatgagcc caatgggatt cggctgaaat accttgttct tccgcctctt tttccattac
     1321 ttgacgacag atgactaaat cacccaatag tggcatttcg attccttcgg gggcctcaaa
     1381 cgggaaagat agcacattgg ttggcttgtc tttaccacga taagtcagat ttagctcgtg
     1441 gctttctgct tcatcgacaa tccgaatggt gatctcactc tcagggaaat catcggtttt
     1501 tgcctcaact gcgagggctt tttgtaccca agtataaaat tgttgttcac ttggtaagtt
     1561 ttcggtattt tcgcaggcga tctgcaaatc aatatataaa ttttccattc gttacctttt
     1621 gcaaattatt gccatttttt ggcggcttgt tggtcactct cacgcccttc aatccaacgt
     1681 tcaccggctt gggtttgttc tcgtttccaa aatggggctt tggttttgag ataatccata
     1741 ataaactcgt tggcgtggta ggcatcacct cgatgagcag aactcacacc gactaatacg
     1801 atttcatcac cggtatgcag ttgcccaatg cggtgaatca ccgcaacgcg ttgtaaatcc
     1861 caacgagtgc gagcttgagc cacaatttct tgcaatgctt ttttagtcat tgccggatag
     1921 tgttcaagaa acaaaccgga aacattatcg cccaaattca tttcacgtac tttaccgatg
     1981 aatagagtgg ctgcccccac gctatgttgt tcactgagcc attggtaaat gctgttttgg
     2041 tcaaattcag cttcttgtac ttcaatcagg gtttcaaaca ttttagcccc cggttaccgg
     2101 tgggaagaat gccacttcat cgccgtcttt gatctccgtt tcaagcgggc agatcgtttg
     2161 attaattgca accagcagtt tgcctttttc aagggcaagt tgccattttt taccctgttg
     2221 gcttaagtgt tcacgcaacg cttcggcagt ggtaaaagta ggctcaacat ctagtctatc
     2281 cgttgccaca agttcacgca cttgggcgaa aaaaagtatc ttaatcaacc ttgaaatctc
     2341 ctgattttcc gccgctttta ctaagtaatc ttacattttc aataatcata tctttttgca
     2401 cggctttaca catatcgtaa atagtcagag cagcaacact agctgcggtt aatgcctcca
     2461 tttccacgcc ggttttgccg gttagtttac agagtgattc aattcgcact tggttgcttt
     2521 ccggcaacgc ctccagttga acttccactt tagaaagcaa taacgggtgg cagagtggaa
     2581 tcagctccca agttctcttc gccgcctgaa taccggcaat tcgagcggtt gcgaatacat
     2641 cgcctttgtg gtgattacca ctgacgatca tttgtaatgt ttcggcattc attcgcacaa
     2701 atgcctccgc tcgggcaatg cgtacggttt cttgtttcat tgaaacatca accatattgg
     2761 cttcgccgtt ttggttgatg tgggtgaagg ttgtcatttg tttgtctctt taaatttgat
     2821 tgtaaaatta atattg
//
LOCUS       DANKBV010000197         2827 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.197, whole genome shotgun sequence.
ACCESSION   DANKBV010000197 DANKBV010000000
VERSION     DANKBV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.197"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..440)
                     /locus_tag="PWY43_002377"
     CDS             complement(<1..440)
                     /locus_tag="PWY43_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HDL2137207.1"
                     /translation="MMAETTSRRDFLKHTALVGAGLLSAPAFALESRQISEKSQNQYD
                     FYGKHQAGIVSPAQKHIYFLVLDLDTTDIAKVKTLFQNWTEYSRNLTLGKNVKPYSAN
                     AYVPPADTGEADSLSAQNLTLTFGVSANFFTKLGIEKHKPKELN"
     gene            complement(451..1263)
                     /locus_tag="PWY43_002378"
     CDS             complement(451..1263)
                     /locus_tag="PWY43_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HDL2137208.1"
                     /translation="MRLTKLAFALSGFFVATQAAALDLTKETEAYKQFVVEQIDQLVT
                     DTEKFVGYLNKGDVQKAKKIYPLARMYFERSEPIAESFGDLDPRIDARLADLAEEGKT
                     EKDWSGFHKIEKVLWQKNTTKGTKATAEQLLKDVKELRAKIPTAEVTPELMITGAVDL
                     LNEVSTTKVTGEEEIFSKTDLYDFKANIEGAEKIYEIFKTQLEQIDAKLSTEIGSRFA
                     AVNALLAKHNKSKTGYDYVPYNKLSKADIKALAEAVNQLGEPLAQLGVLLNK"
     gene            1451..1693
                     /locus_tag="PWY43_002379"
     CDS             1451..1693
                     /locus_tag="PWY43_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL2137209.1"
                     /translation="MSRTTSVTLAEPLSQFVNQMVESGRYGSTNEVISAALKILEAQE
                     QQLIQLRALIDEGLESGVSEETVQSIISKAKAKRNV"
     gene            1932..>2827
                     /locus_tag="PWY43_002380"
     CDS             1932..>2827
                     /locus_tag="PWY43_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2137210.1"
                     /translation="MSKQGKPFILHSPFKPSGDQPSAIAKLTEGLNDGLAHQTLLGVT
                     GSGKTFTIANVIAKLNRPAMVLAPNKTLAAQLYAEMKAFFPENAVEYFVSYYDYYQPE
                     AYVPASDTFIEKDASINEQIEQMRLSATKSFLERRDTIVVASVSAIYGLGDVDAYMQM
                     MLHLQVGAMIGQREILSRLAELQYTRNDQAFQRSTFRVRGDVIDIYPAESDEVALRVE
                     LFDDEIENLSLFDPLTGRSLGKVPRYTIYPKTHYVTPRERILEAIEQIKVELADRRTY
                     FIKENKLLEEQRIAQRTQFDIE"
BASE COUNT          793 a          590 c          592 g          852 t
ORIGIN      
        1 tcattcagtt ctttcggctt atgtttttca atgccgagct ttgtaaagaa attggcactg
       61 acgccaaaag taagagtgag attctgtgcc gaaaggctat ctgcctcacc ggtatccgcc
      121 ggtggaacat aagcattagc actataaggt ttcacatttt tgccaagcgt gaggttacgg
      181 ctatattccg tccagttttg aaacaatgtt tttactttgg caatgtcagt ggtgtctaaa
      241 tccagcacta aaaagtagat atgtttttgt gcagggctaa caataccagc ttgatgtttg
      301 ccgtaaaagt cgtattgatt ttgagatttt tcagatattt gtcgtgattc caaagcgaaa
      361 gcaggagcag acaataagcc tgctcctact aatgcggtat gttttaaaaa atcacggcga
      421 gaagtggttt ctgccatcat ttttgctcca ctatttattt aaaagtacgc caagttgagc
      481 taacggctcg cctaattggt ttacagcctc tgctaacgct ttaatatccg cttttgacag
      541 tttgttataa ggcacataat cataaccggt tttagattta ttgtgttttg ctaacagcgc
      601 attgacagcc gcaaaacgag agccgatttc tgttgagagt ttggcatcaa tttgctctaa
      661 ttgcgtttta aaaatctcat agattttttc tgcaccttca atattggctt taaaatcgta
      721 aaggtcggtt ttagagaaaa tttcttcttc accggttact ttagtagtag aaacttcgtt
      781 taataaatct accgcaccgg taatcatcag ctcaggggta acttctgcag tgggaatttt
      841 cgcacgcagc tcttttacat cttttaataa ttgttctgcc gttgcttttg tgcctttagt
      901 ggtgtttttc tgccacaata ctttctcgat tttatggaaa cctgaccaat ctttctcggt
      961 tttaccttct tcggctaaat ctgccagacg ggcatcaata cgagggtcta aatcgccaaa
     1021 actttctgca atcggttcag aacgttcaaa atacattctt gcaagcgggt aaattttctt
     1081 cgctttttgc acatcgcctt tgtttaagta gccgacaaat ttttccgtat cggtaaccag
     1141 ttggtcaatc tgttctacca caaattgttt ataagcctcg gtttccttag ttaagtctaa
     1201 agctgccgct tgagtagcca caaaaaagcc agaaagtgca aaagcaagtt tggttaaacg
     1261 catagaaatt tccttttaat aaaaatattg cgacataata tccttattgc gaattattcg
     1321 caaaaaataa tgctgatttt gtgatgtaaa tcacattttg ttttgcaaat tattgctaaa
     1381 aatagaccgc ttgcttatct aaattagagc cattacaatc tgctcaataa tcaataagat
     1441 aggaatagta atgagtagaa caaccagcgt aactttagct gagccattaa gccaatttgt
     1501 gaaccaaatg gttgaaagtg ggcgttatgg ttcgaccaac gaggtcatca gtgccgcatt
     1561 aaaaatattg gaagcacaag aacagcaact aattcagcta agagccttaa ttgatgaagg
     1621 cttagaaagc ggtgtttctg aggaaaccgt tcaatctatt atcagcaagg caaaggcaaa
     1681 acgtaatgta taaactttct gttagggcag agcgagattt agcgttactt tattcgccct
     1741 ttgttacgct gtcattacca ccgctcacat tctatttatt ataaaccaat cccaaaaggt
     1801 atctttattg ttagggtgtt agatcaaaga atggatcaat ataagcagtt acaataaatt
     1861 tgcaaaaatc ccgataaaaa cgaccgcttg cttgtgctaa aataaaccga ttttttaaga
     1921 caggattttc tatgagcaag caaggcaaac cctttatcct ccattctcct ttcaagccct
     1981 ccggcgacca accaagtgcg attgccaaat tgaccgaagg cttaaatgac ggcttggcac
     2041 atcaaacctt acttggcgta accggctcgg ggaaaacctt taccattgct aatgtgattg
     2101 ccaagctcaa tcgcccggcg atggtgcttg ccccgaataa aacccttgcc gcccagcttt
     2161 atgccgagat gaaagcgttc tttccggaaa atgcggtgga gtatttcgtc tcttactatg
     2221 attactacca gcccgaagcc tatgtgccgg caagcgatac ctttatcgag aaagatgcct
     2281 ctatcaatga gcagatcgag caaatgcgac tttccgcaac caaatccttt ttggaacgcc
     2341 gagatacgat tgtagtggcg tccgtttctg ccatttacgg cttgggtgat gtggacgctt
     2401 atatgcagat gatgttgcat ttgcaagtgg gggcgatgat tgggcagcgt gaaattctct
     2461 ctcgccttgc cgaattgcaa tatactcgca acgatcaggc gttccaacgt tcgactttcc
     2521 gagtgcgggg cgatgtgatt gatatttacc ctgccgaaag cgatgaagta gcgttaaggg
     2581 tagagctgtt tgacgatgaa atcgaaaatc tgtcgctgtt tgatccgctt acagggcgta
     2641 gcttgggcaa ggtgcctcgc tataccattt acccgaaaac ccactatgtg actcctcggg
     2701 aacggatttt ggaggcaatc gagcaaatta aggtcgagct tgccgaccgt cgcacctatt
     2761 tcatcaaaga gaataaattg ctggaagagc agcggatcgc ccaacgtacg cagtttgata
     2821 ttgagat
//
LOCUS       DANKBV010000198         2817 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.198, whole genome shotgun sequence.
ACCESSION   DANKBV010000198 DANKBV010000000
VERSION     DANKBV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.198"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            421..615
                     /locus_tag="PWY43_002381"
     CDS             421..615
                     /locus_tag="PWY43_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137211.1"
                     /translation="MALSRTEIVNRSNAKRGIKLKAFKLHESTIAQIEQLAQQLNIPQ
                     NQLIKLAVEKLQEQTNFTTG"
     gene            complement(583..948)
                     /locus_tag="PWY43_002382"
     CDS             complement(583..948)
                     /locus_tag="PWY43_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137212.1"
                     /translation="MQIDVKGVLNLWGGYVRFTDCKGYPTMQAFMRESPQERTKQRYL
                     ARLDDDTLEKVDRQMRKLQANDKRQFDVLFYKYAVGLENKQVWQKLSLSKTSYHTELL
                     LAERFMEGALAGSEIRLLL"
     gene            complement(938..1306)
                     /locus_tag="PWY43_002383"
     CDS             complement(938..1306)
                     /locus_tag="PWY43_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL2137213.1"
                     /translation="MSDWLEIALPYPPSVNHYWKHTRNGRHYISDKGKAFRAEVVKIC
                     KMFDPFKGAVQTVLQIYYPDKRKRDPDNLEKALWDALKIGGLIEEDDNQILVDKRTIT
                     AGFKKGGMVVLKIKAVGNAN"
     gene            complement(1303..1587)
                     /locus_tag="PWY43_002384"
     CDS             complement(1303..1587)
                     /locus_tag="PWY43_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDL2137214.1"
                     /translation="MANLRKEAKGRECQVRLPGICNHNPETVVLAHYRMAGLNGVGMK
                     PDDIFGAWACSSCHDECDRRTRKLEADYVRQAHAEGVFRTQAILRREGKL"
     gene            complement(1964..2176)
                     /locus_tag="PWY43_002385"
     CDS             complement(1964..2176)
                     /locus_tag="PWY43_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137215.1"
                     /translation="MKKNVIRYWLSVPFRIIAVLISLSILTLRYIGYLLIYPLAFFVA
                     VGSFIVIGQWKYQECLDDFKSDFNPI"
     gene            complement(2226..2678)
                     /locus_tag="PWY43_002386"
     CDS             complement(2226..2678)
                     /locus_tag="PWY43_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL2137216.1"
                     /translation="MAKYQMMKLPGGILSPLNDEEAERLKSFQNGEQYEIEIKRQRNP
                     AFHRKVFAFFNFCFDCWSGSHSDWEFQDPTAQFDTFRKHLTVMAGFYVQTWNLKGQVR
                     IEAKSLSYANMEQDEFEQAYKALINAAIKHVFNNTKDENTINQLYSFF"
BASE COUNT          887 a          684 c          426 g          820 t
ORIGIN      
        1 cgtaaacaaa aaagccccaa gcatttctgc tcagggctgt aaaattcttt taagttcacc
       61 acctatgcaa tgaaccgcaa cttaccataa ataatataga ttaagtccga actttgcaac
      121 actttttaat catttaaatg caaaaaagcc cataatttac aaaaccatgg gctttttaac
      181 gatattttgc ttgaatatat tggttatcca acatataata aacttgtttt cagtaatagt
      241 gctggaaacg agccggtggc gacctttaat cccaccgact tttggaggga taacaaatgc
      301 tttaccgcat tatcctagtt atcattctct tagtgattag cctgccagca tactaattga
      361 atgaaactaa gtagggggag aaatctccct actcttcaaa acctatatta aggatttatg
      421 atggcactgt caagaaccga aattgtaaac cgtagcaacg caaaacgggg aattaaactt
      481 aaagccttta aactgcacga aagcacaatc gcacaaatcg agcaacttgc ccaacagtta
      541 aatattcccc aaaatcaact aattaaatta gccgttgaga agctacaaga gcaaacgaat
      601 ttcactaccg gctaatgccc cctccataaa tcgttcagca agaagtaatt ctgtatgata
      661 tgaagtcttg ctgagcgata acttctgcca aacctgctta ttctctaacc ctactgcata
      721 tttataaaat agtacatcga attgtcgctt gtcgttcgct tgtagcttcc gcatttgccg
      781 atcgactttc tcaagcgtat catcatctaa gcgggctaaa taacgctgtt ttgttctctc
      841 ttgtggagac tcacgcataa atgcctgcat tgtcggatag cccttgcagt cggtaaaacg
      901 cacatatcca ccccataaat ttaatacacc ctttacatca atttgcatta cccaccgcct
      961 taatctttaa caccaccatt ccccctttct taaaacctgc cgtaatggtt cgcttatcga
     1021 ctaaaatttg attatcgtct tcttcaatta agccaccgat ttttaatgcg tcccacaagg
     1081 ctttttctaa attatctgga tcacgcttgc gtttatcagg atagtaaatc tgcaataccg
     1141 tttgtaccgc tcctttaaac ggatcaaaca ttttgcagat tttcaccact tccgccctaa
     1201 aggctttccc cttatctgaa atataatgcc taccgtttcg ggtatgcttc cagtaatggt
     1261 tcacactcgg cggataaggc aacgcaattt caagccaatc actcataatt tcccttctct
     1321 ccgtaaaata gcctgcgttc taaatacgcc ctctgcatga gcctgtctca cataatccgc
     1381 ttctaatttg cgagttctac ggtcgcactc atcgtggcag ctactgcaag cccacgcccc
     1441 gaaaatatca tcgggtttca ttcccacacc gtttaatcct gccattcgat agtgagccaa
     1501 taccaccgtt tccggattat gattacagat acccggtaaa cggacttggc actctctgcc
     1561 ttttgcctct ttgcgtagat tagccattcc aacctccttg gtctctttta tcaaaccact
     1621 caataaaaaa gagtgaaccg gcacatacac taagaatagc gatagtgatt aaaattacag
     1681 tcagttcatt gcactggctc acttattaat ctccttaaat taaataacat tccacataaa
     1741 aggaataatc cgcaaccgat aacaaacgat attaaaatcc aaaaccctaa ctgaataatc
     1801 accataaacc acggtgcgtt tttaatattc attaaagtag tattaatcgc caataaacag
     1861 cttggaataa tgccaaataa catcacgagt gcgatagttt caaagaattt ttgtcatttt
     1921 gatttctcct acccccgaat tcgagggaat taataaacac acactaaatc ggattaaaat
     1981 cgctcttaaa gtcatcaagg cattcttgat atttccattg accgataaca ataaaactac
     2041 cgactgccac aaaaaatgct aatggataaa tcaataagta cccgatatat ctaagagtaa
     2101 gaattgacaa agaaataagc actgcaataa ttctgaatgg cacgctaagc caatatctaa
     2161 taacattctt tttcatttta atctcctgca attaaaccta attaaatatg gcgatttcgc
     2221 cttaattaaa aaaacgaata caactgattt atcgtattct catctttggt attgttaaaa
     2281 acgtgcttga tagctgcatt aatcaacgct ttataagcct gctcgaactc gtcttgttcc
     2341 atattggcgt agcttaggct tttcgcctca atccgcactt gcccttttaa attccacgtc
     2401 tgcacataaa accctgccat tacagttaaa tgcttgcgga aagtatcaaa ttgagcggtt
     2461 ggatcttgga actcccaatc ggaatggcta ccactccagc aatcaaaaca gaaattaaag
     2521 aacgcaaaca ccttgcgatg aaaagcggga ttacgctggc gtttaatctc gatttcgtac
     2581 tgctcgccat tctgaaacga tttcaaccgc tcggcttctt catcgtttaa cgggcttaaa
     2641 atcccacccg gcaatttcat catttggtat ttagccatct ctatccctca atgaaaatcc
     2701 aaaagctcta aataccaaat atgctaacca cccgaagaaa attagaatcc ctaaaaatcc
     2761 aatagtagaa tcactcatca cacttcccac ccttcgttat ttttcatagc cgcccag
//
LOCUS       DANKBV010000199         2751 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.199, whole genome shotgun sequence.
ACCESSION   DANKBV010000199 DANKBV010000000
VERSION     DANKBV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.199"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..576
                     /locus_tag="PWY43_002387"
     CDS             <1..576
                     /locus_tag="PWY43_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL2137217.1"
                     /translation="RKFIAVQYPEEIDIKSKNGKVAKQFCQRKNLPLYITEISKERIR
                     RAGKQILQNNPENRPLDVGFKVFKLTESHFKQWQSPTSENLADQLEMFIDPVSEKADT
                     QAVLYELLLRLGLKLTAKVRLENKVFWVEENGLIFALLLNAADEEIIQTVIAQQPKKV
                     IALDRLFNGNDARKKNTELQMQDAGITFFVI"
     gene            586..>2751
                     /locus_tag="PWY43_002388"
     CDS             586..>2751
                     /locus_tag="PWY43_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2137218.1"
                     /translation="MELQFETLEYQLQAVNATVNLFVGQPNAATEFSLKAQNDMRFVP
                     NLGLQISDEQLQQNLANLQNRQKIDRTLLVEQGKNFTVEMETGTGKTYVYLRTIFELN
                     RQYGWQKFVIVVPSVAIREGVLHTLETTKSHFNTVFDNPSVNQKFEYKSNQISRLKSF
                     ASANHIEILVMNIDSFTKESNVINTVNESGDAPIFYIQQANPIVIIDEPQNMETEIRR
                     NAIESLSPLFTLRYSATHKNAYNPIFSLNPVQAYEMGLVKQIEVDSVLAENEVNGAYL
                     SLKEIKTGAKSWSAKIEISFNDKKAMKKKVISVKPNQDLYELSNQNEMYRSGFILSGM
                     DTDSQMVSFSNGTQIFQGAEHSQLKEDVQKMQIRRTIGEHLQKEKKLRPLGIKVLSLF
                     FIDKVDNYRPSAQSPTGGKFAKWFEEIYLELAKEEPQGVHNGYFSQDKKGVAKDTNGT
                     TQADNETYQLIMRDKEKLLSLENPLRFIFSHSALKEGWDNPNVFQICTLNDTASEMKK
                     RQEIGRGLRLPVNQQGLRIYDRHLNTLTIIANESYEQFAATLQREIEEDCGVQFAKSN
                     VKKREDKKAVRYRKDFSLHPEFAAIWERIKHQTTYRVDFSNAELIESAVEKIRKMPDI
                     DATKIKHSKVKLEMNETGISTNYRSSNETAVNKQWAIPDLLSEIQKKTGLTRATLCKI
                     LQKSDRLQEVFRNPQRFIDLIAEQINLALQQIMTDGVEYH"
BASE COUNT          949 a          489 c          592 g          721 t
ORIGIN      
        1 agaaaattta ttgcggttca atatcctgaa gaaattgata tcaagagtaa gaatggaaaa
       61 gtagcaaagc aattttgcca aagaaaaaat ttaccactat atataactga aatttcaaaa
      121 gaacgtattc gccgtgccgg aaagcagatt ttgcaaaata accccgaaaa ccgaccgctt
      181 gatgtaggtt ttaaagtctt caaactgaca gaaagccatt ttaaacaatg gcaatcgccc
      241 acatctgaaa atttagccga tcaacttgaa atgtttattg atcctgttag tgaaaaagcg
      301 gatacgcaag ccgttttata tgagctttta ttgcgtttag ggttaaaact cacggcaaaa
      361 gtgcggttgg aaaataaggt gttttgggtg gaagaaaacg ggctgatttt cgccttgctg
      421 ttaaatgcag ccgacgaaga aattattcaa actgttatcg ctcaacaacc gaaaaaagtc
      481 atcgctttag accgtttatt caacggcaat gatgcccgga agaaaaacac cgaattgcaa
      541 atgcaagatg ccggcattac gttttttgtg atttaaggag aaaaaatgga actccaattt
      601 gaaaccttag agtatcaatt acaagccgtt aatgccacag taaatttgtt tgtcggacag
      661 ccgaatgccg caactgaatt ttcactcaaa gcccaaaatg atatgcggtt tgtgccgaat
      721 ttaggcttgc agattagtga tgagcagtta caacagaatt tggctaatct gcaaaacagg
      781 caaaaaatcg accgcacttt gcttgtcgaa cagggaaaaa attttaccgt ggaaatggaa
      841 accggtacag ggaaaaccta tgtctattta cgcacgattt ttgaattaaa ccgtcaatac
      901 ggctggcaaa aatttgtgat tgtcgtgccg agtgtggcaa ttcgtgaggg agtgttgcat
      961 acgttggaaa ctacaaaaag ccattttaat acggtatttg ataacccgag cgtcaatcaa
     1021 aaatttgaat ataaaagcaa ccaaatttca cgcttaaaat cctttgctag tgctaatcat
     1081 attgaaattt tagtgatgaa tattgattct ttcaccaaag aaagtaacgt gattaatacc
     1141 gtgaatgaaa gcggtgatgc tccgattttc tatattcaac aggcgaatcc gattgtgatt
     1201 attgatgaac cgcaaaatat ggaaacggag attcgccgta atgcgattga gagtttaagt
     1261 ccgcttttca ctttgcgtta ttctgccacc cataaaaatg cgtataaccc gatttttagc
     1321 ctcaatccgg tgcaagccta tgaaatggga ttagttaaac aaattgaggt agatagcgta
     1381 ttggcggaaa atgaggtaaa tggagcttat ctttccctca aagagattaa aacaggggca
     1441 aaaagctggt cggcaaaaat cgaaatttca tttaacgata aaaaagcgat gaaaaagaaa
     1501 gtgattagcg tgaaaccgaa tcaggatttg tatgagcttt ccaaccaaaa tgaaatgtat
     1561 cgttcagggt ttatattaag cggaatggat accgatagcc aaatggttag cttctccaac
     1621 ggcacacaaa tttttcaagg ggcggaacat tcccaactta aagaagatgt gcaaaaaatg
     1681 cagattcgcc gcactattgg cgagcatttg caaaaagaaa agaaattacg accgcttggg
     1741 atcaaggtgt tgtctttgtt ctttatcgat aaagtggata attatcgccc ttctgcacaa
     1801 agccctacgg ggggaaaatt tgcaaaatgg tttgaggaaa tttatttgga gctagccaaa
     1861 gaagagcctc aaggcgtgca taacggttat ttctcacagg ataaaaaagg cgtagcaaaa
     1921 gatactaatg gcacaacgca agcggataac gagacttatc aactgattat gcgggataaa
     1981 gaaaaattgc tttcgctgga gaatccgtta cggtttattt tctcgcattc tgccttaaaa
     2041 gaaggatggg ataatccgaa tgtgtttcaa atttgtactt taaacgatac tgcaagcgaa
     2101 atgaaaaaac gccaagagat tgggcggggc ttgcgtttgc ctgtgaatca acaaggattg
     2161 cggatttatg atcgtcatct aaatacgctg acaattatcg caaatgaaag ttacgaacaa
     2221 tttgccgcca cattacagcg tgaaattgag gaagattgtg gtgtacagtt tgccaaaagc
     2281 aatgttaaaa aacgggaaga taaaaaagcc gttcgctacc gtaaagattt cagcttacat
     2341 ccggaatttg cggcaatttg ggaacggatt aaacaccaaa ccacttatcg ggtagatttc
     2401 agtaatgcgg aattgattga aagtgcggtg gaaaaaatcc gcaaaatgcc cgatatcgac
     2461 gcgacaaaaa ttaagcattc caaagtgaaa ttggaaatga atgaaacagg gatttcaact
     2521 aattatcgct catctaatga aacagcggta aataaacaat gggcgatacc tgatttattg
     2581 agtgagattc aaaagaaaac cgggctgacg cgtgcgaccc tttgcaaaat tttacaaaaa
     2641 tccgaccgct tgcaagaagt cttccgcaat ccgcaacgct ttattgattt aattgcagag
     2701 caaatcaacc ttgctttaca acaaattatg acagacgggg tggaatatca c
//
LOCUS       DANKBV010000200         2616 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.200, whole genome shotgun sequence.
ACCESSION   DANKBV010000200 DANKBV010000000
VERSION     DANKBV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.200"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(116..191)
                     /locus_tag="PWY43_002389"
     tRNA            complement(116..191)
                     /locus_tag="PWY43_002389"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Thr,seq:ggt)
     gene            complement(195..269)
                     /locus_tag="PWY43_002390"
     tRNA            complement(195..269)
                     /locus_tag="PWY43_002390"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Gly,seq:tcc)
     gene            complement(313..397)
                     /locus_tag="PWY43_002391"
     tRNA            complement(313..397)
                     /locus_tag="PWY43_002391"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Tyr,seq:gta)
     gene            complement(432..507)
                     /locus_tag="PWY43_002392"
     tRNA            complement(432..507)
                     /locus_tag="PWY43_002392"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(472..474),aa:Thr,seq:tgt)
     gene            754..1704
                     /gene="coaA"
                     /locus_tag="PWY43_002393"
     CDS             754..1704
                     /gene="coaA"
                     /locus_tag="PWY43_002393"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL2137219.1"
                     /translation="MSLEKTSKITPFLTFDRQQWADLRKSVPLKLTEQDLKPLLGFNE
                     DLSLEEVRTIYLPLARLIQYYIEENLKRQTVLHRFLGIEPPKVPYIISIAGSVSVGKS
                     TSARILQALLSHYPKERKVDLITTDGFLYPLATLTEKNLLKKKGFPESYDIHSLIRFV
                     SDIKSGKRNVKAPIYSHLTYDIIPNQFNTVDQPDIVILEGLNVLQSGMNYPSSPHNVF
                     VSDFVDFSIYVDAEESLLKKWYINRFLKFRRSAFADPNSYFHHYASLSEQEAIETAST
                     IWEEINGLNLRQNILPSRERANLILIKGQDHAIETVKLRK"
     gene            1843..2382
                     /locus_tag="PWY43_002394"
     CDS             1843..2382
                     /locus_tag="PWY43_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HDL2137220.1"
                     /translation="MNKKFLLPLILFLALSVAFLVQLARNSQGDDPKKLESALIGKTV
                     PSFNLESVLDEKQTIDQSIVKTGKPRLLNVWATWCPTCYAEHEYLTILAKQGVEIVGI
                     DYKDERPKAIKFLANYGNPYKAIIFDPKGSLGLDLGVYGAPETFIIDGNGVIQYRHAG
                     DVNDQVWNQVLKPIYEKLK"
BASE COUNT          835 a          538 c          471 g          772 t
ORIGIN      
        1 tttctaaaag cctcttaaac aaaaacggtt acaatggaac aaaagaaccc attaaccata
       61 atgtagaaat taataccaat aaaaatttgc gtgagaaaga tagaatttag agaagtggtg
      121 ctgataggcg gatttgaacc gccgacctca cccttaccaa gggtgcactc taccaactga
      181 gctatatcag cgcttggagc gggcagcggg aatcgaaccc gcatcattag cttggaaggc
      241 taaggtaata gccattatac gatgcccgct gttctaaatt cgtctgacct acgcgattca
      301 aaatatcaga gatggtggag ggagaaggat tcgaaccttc gaaggctgag ccaacagatt
      361 tacagtctgc cccctttggc cgctcgggaa cccctccacg ataattgaat acttatttac
      421 gcctgagtta atggtgccga ctaccggaat cgaactggtg acctactgat tacaagtcag
      481 ttgctctacc tactgagcta agtcggcgtt gctgtgtcgt aagcaagtga tgtgcattat
      541 agggaaattt taacagcgtg caagtgtttt ttaaaaaaat attaaaaaaa gcgttctttc
      601 gatttttaaa tatacaaaat gactattttt tgtaaaatac aacggatttt ctctgcataa
      661 ctacggcaaa ataagtgcat atattgtata gttacgccct taatttggta tttaattacc
      721 ttaatattcc agcaaaaaat tgagattttg gctatgagcc ttgaaaaaac cagcaaaatt
      781 actccttttc tcacttttga tcgccaacaa tgggctgatt tacgcaaatc cgttccattg
      841 aagctgaccg agcaagacct aaaaccgctt ttaggcttta atgaagatct ttcattagaa
      901 gaagtacgca ctatttattt accgcttgcc cgtttaattc aatactatat agaagaaaac
      961 ttaaaacgcc agacagtctt acatcgcttt ctcggcattg aaccgccaaa agtcccttac
     1021 attattagta ttgccggcag cgtatcggtt gggaaaagta cctctgcccg tattctgcaa
     1081 gctctgctct cccattaccc taaagagcga aaagtggatt taattaccac cgacggtttt
     1141 ttatatccac ttgcaacgct aaccgaaaag aacttactca agaaaaaagg ctttccggaa
     1201 tcttatgata ttcacagcct gattcgcttt gtatctgata ttaaatccgg caaacgcaat
     1261 gtgaaagccc cgatttattc gcacctaacc tacgatatta tcccaaatca atttaatacg
     1321 gtggatcaac ccgatattgt gattttagaa gggctaaacg tgttacaaag tggaatgaac
     1381 tacccatcca gcccacataa tgtatttgta tcggactttg tggatttttc tatttatgtg
     1441 gatgccgaag aaagtttgct gaaaaaatgg tatatcaacc gtttcttgaa attccgtcgc
     1501 agtgcctttg ccgacccaaa ctcctatttc caccattatg cgagtttgtc cgagcaagaa
     1561 gcgattgaaa ccgcctcaac gatttgggaa gagatcaatg gcttaaattt acgccaaaat
     1621 attctcccaa gccgagagcg tgctaacctt attttaatca aagggcaaga ccacgccatt
     1681 gaaacggtaa aattacgcaa ataatttaac tttttcgacc gcttgtaata ccttaaaatc
     1741 cgttgaatac cactcaacgg attttttatt taaatcaatg cttattcaac tattttttgg
     1801 taagctaacg actttgaaag tgattgatat taggcatata caatgaacaa aaaatttcta
     1861 cttcctttaa ttctcttttt agcgttatct gtggcatttt tggttcaact cgctcgcaac
     1921 tcgcaaggcg acgatccgaa aaaattagaa tcagccttaa tcggcaaaac agttccgagc
     1981 tttaatttag aatcggtatt agatgaaaaa cagacaatcg accaaagtat cgtcaaaacc
     2041 ggtaaacctc gcctgcttaa cgtgtgggca acttggtgtc cgacttgtta tgccgaacac
     2101 gaatacttaa ccattttggc aaaacaaggg gtggaaattg tcggtattga ttataaagac
     2161 gaacgcccaa aagccatcaa attcttagcc aattacggca acccttataa agccattatt
     2221 tttgacccga aaggttcact tggtttagat ttaggtgttt atggtgcacc cgaaaccttt
     2281 attattgacg gtaacggcgt gattcaatac cgccacgccg gcgatgtaaa cgaccaagtc
     2341 tggaaccagg tgcttaaacc gatttatgag aaattaaaat aaaaatgaaa tactttttga
     2401 aacagaagaa agtaaaatcc tcttttacca gggaatataa tatattagca agcatttctt
     2461 tgattgtaac tctattaatt attttcagcc ttattttagg aggtatattc cttttcatat
     2521 atcttaaaaa attagacaaa tcattcttat ttatcaacct actgaatgat aaacttttac
     2581 atgcattaac acttacatca ttatgcataa ttctta
//
LOCUS       DANKBV010000201         2556 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.201, whole genome shotgun sequence.
ACCESSION   DANKBV010000201 DANKBV010000000
VERSION     DANKBV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.201"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..114)
                     /locus_tag="PWY43_002395"
     CDS             complement(<1..114)
                     /locus_tag="PWY43_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HDL2137221.1"
                     /translation="MITVKNLHHKIGNATILNNINLTIPNGGITALIGANGA"
     gene            complement(134..1012)
                     /locus_tag="PWY43_002396"
     CDS             complement(134..1012)
                     /locus_tag="PWY43_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2137222.1"
                     /translation="MTYNAKGNWDFILQLRGKKLILLCTIAYTIGISTLLFQTLTHNP
                     ILTPSILGFDSLYLLLQTSLVFLFGGLGFTQLDPSYKFFFETLLMLGASILLFSSLNR
                     NRSDLTRMLLIGVIFGVLFRSLNNLLQRMIDPTEFAVAQGTSFASFNLTTPTLLWIGG
                     CIAILSAIWVWLQRHKLDILLLGKDKAIGLGLEYHQFSRQLLICCGLLVSVSTALVGP
                     ILFLGLLVCAIVNAISPTVCHSVRIPMTFLISAITLILGQAIFEQVLKMQGVLSVVIE
                     FIGGIVFIYLILKRKK"
     gene            complement(1065..2033)
                     /locus_tag="PWY43_002397"
     CDS             complement(1065..2033)
                     /locus_tag="PWY43_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2137223.1"
                     /translation="MFSFFRLNLFLLILLIPISASVGVADFHWSEIFHSSEQSQIFFI
                     SRLPRTLAVILVGATLAVAGMVLQIVLKNRFIEPSMIGTSQSAAIGILLASLLFPTSA
                     LLLKMSIATLSALMGMGLFMLLIRQLPPHQQLMLPLIGIVFGNVIEAISTFIAYETDS
                     LQVLSIWFSGDFSGILAGRYELLWITAILAVVVYVMADQLTIAGLGENISTNLGINYK
                     QMTWLALIVVAMITAIVVVTVGQIPFIGLVVPNIVSRIAGDRLRKNLPAVVVLGANLV
                     LICDILGRAINAPYEVPISTIFGIIGTVIFLYLLLKGKRAEEKARG"
     gene            complement(2127..>2556)
                     /locus_tag="PWY43_002398"
     CDS             complement(2127..>2556)
                     /locus_tag="PWY43_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2137224.1"
                     /translation="INTLLDQTKVAVKGKGNGLIILVNGGKISAFGSGYRLSWIHDNL
                     GIPMADSSIKNTGHGQPVSFEFIQKLNPDWLFVLDRIAAIGQEGQSAQQVLDNELVRQ
                     TKAWKNGNIVYLSAASYLAPGGYEQMKTDLTNIKTAFEKK"
BASE COUNT          876 a          526 c          473 g          681 t
ORIGIN      
        1 cgcaccattt gctccgatta aagcggtaat accaccattt ggaatggtta aattgatatt
       61 attcagaatc gtggcattcc cgattttgtg gtgaagattt ttaaccgtta tcattttctt
      121 ccttaattta tggttatttt ttccgtttta aaatgagata aataaagaca ataccgccaa
      181 taaattcaat aacgacactc agaacccctt gcattttcag cacttgctcg aagattgcct
      241 gccctaaaat taaggtgatg gcagagatta aaaaagtcat tggtattcgc acgctatggc
      301 aaacagtcgg gctaattgca ttaacaatgg cacaaacaag tagccctaga aatagaatcg
      361 ggccaacaag tgcggttgaa acagacacca acaatccaca acagattagt aattgacgtg
      421 aaaattgatg atattctaac ccaagcccaa ttgccttatc tttgccaagt aacaaaatat
      481 ctaatttatg ccgttgtaac cagacccaaa tggcagagag aatagcgata cagcctccaa
      541 tccaaagtaa tgtaggggta gtcaggttaa atgaggcaaa agaagtgcct tgagcaacgg
      601 caaactctgt tgggtcgatc attcgttgca acaaattatt taggcttcta aataagacac
      661 caaagatgac accgattaaa agcattcggg ttaaatcgga tcgatttcga ttcagtgaac
      721 taaatagtaa tattgatgcc cctagcatta acaaggtttc aaagaaaaac ttatagcttg
      781 ggtctaactg agtaaagcct aaaccgccaa ataaaaatac taacgaggtt tgcaatagca
      841 agtagagcga atcgaacccc aaaatacttg gagttaaaat aggattatgt gtgagtgttt
      901 ggaatagcag ggtcgaaata ccaatcgtat aagcaatagt acagagcaaa ataagctttt
      961 tgccacgcag ttgcaatata aagtcccaat tacctttcgc attataggtc atataaaaca
     1021 gaatacttgc taataagatt aagcttaaaa cgataaagcg tgatctaacc acgagccttc
     1081 tcctcagccc gtttcccttt gagcaataaa tagaggaaaa tcactgtccc aataatccca
     1141 aaaatagtag aaatcggcac ttcataaggg gcattgatgg ctcgccctaa aatgtcacaa
     1201 atcaggacta aattcgcacc taacaccacg accgcaggca gatttttacg caatcggtca
     1261 cctgctatgc gagagacaat attagggacg accaagccga taaacggaat ctgtccaacc
     1321 gtcaccacga caatagcggt aatcatcgcc accacaatta aggcaagcca agtcatttgt
     1381 ttgtagttaa tgcctaagtt ggtactaata ttttcaccaa ggcctgcaat ggttaattga
     1441 tctgccatca cataaaccac aacagccaga atagcggtaa tccagagcag ttcataccga
     1501 cctgccaaaa taccggagaa atcgccggaa aaccaaatcg aaagcacttg taggctgtca
     1561 gtttcataag caataaaagt gctgatagct tcaataacat tgccaaaaac gatcccgatt
     1621 aatgggagca ttaattgttg atgaggcggt aattgacgaa tgagcaacat aaaaagcccc
     1681 attcccatta atgcacttaa tgtggcaata gacattttta acagtaatgc tgatgttggg
     1741 aaaagtaaac tagcaagtaa aataccaatt gccgcacttt ggctagtgcc aatcatacta
     1801 ggctcaataa agcggttctt cagcacaatt tgtaatacca tacctgcaac ggctaaggtt
     1861 gcaccgacta atatcactgc gagcgttctt ggtaaacggc tgataaaaaa gatctgactt
     1921 tgttcggaag aatgaaaaat ttcactccaa tgaaaatctg ccacacccac actggcactg
     1981 attggaatga gcaatattaa aagaaataga tttaatctaa aaaaagagaa catagtgata
     2041 aatagcaagc ggtcatattc tctctctttt ttacaaatgc aggcacttgc aaaaatagga
     2101 ggaaatctga ccgcttgtaa ggttaattat tttttctcaa atgctgtttt aatgttagtt
     2161 aggtcggttt tcatctgctc ataaccaccc ggcgctaaat atgaggcagc acttagataa
     2221 acgatattac cgtttttcca agctttagtt tgacgaacta attcattatc caacacttgt
     2281 tgggctgatt gaccttcttg tccgatagcc gcaatacggt ctaacacaaa caaccaatcc
     2341 ggattgagtt tttggataaa ttcaaacgaa acaggctgac cgtgaccggt atttttaatg
     2401 ctagaatctg ccattggaat ccctaaatta tcgtgaatcc aacttaaacg ataacctgaa
     2461 ccaaatgcag aaattttgcc accattgact aaaataatca gtccgttacc tttacctttt
     2521 actgccactt tggtttggtc taataaagta ttaatt
//
LOCUS       DANKBV010000202         2501 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.202, whole genome shotgun sequence.
ACCESSION   DANKBV010000202 DANKBV010000000
VERSION     DANKBV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.202"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            203..682
                     /locus_tag="PWY43_002399"
     CDS             203..682
                     /locus_tag="PWY43_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137225.1"
                     /translation="MYVQKHSNYSTALLVVIREKINSLPHQGLILDLISISKDDLDDI
                     LEGNTDMNIHQLLSLIYKAGFFDVLNITNNLCIKLSQFGFYFQNGNLSQKDDELLSLY
                     REFYKHNEFITHLKSHSLYGCIEQPTSAFFHYCTNENFRNAFNEESLYDFQMWNVFR"
     gene            692..1861
                     /locus_tag="PWY43_002400"
     CDS             692..1861
                     /locus_tag="PWY43_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137226.1"
                     /translation="MSSQNSFLTKYSKSNSHSQDSQKINESLEWLNEKNYGELYSSFL
                     IAVGVKEFLDKGFIPNDNYRDIIALLPVALYVFHLRSLKHIKNLESNSYLDEEGRDTV
                     NKYKAFFSIFEAEIRDATCICDNKIVTINGEVCCDIKEVARKIVIEFLNEVDIGNKVK
                     FYYPDERHELFVELLIVLATLRIYRNRNIFFFEKGFFNFFPEFITILGTGAFNGFLDF
                     PSLVGKVFPEIIDTINEGLDLDIAIENFEVLSQEEMTECMGLYEQALIKKAEEGEIGL
                     LNVDEINSFYEDNSELILLAKQPMVEEIIPKPDSKASSAILKFFDSNSNTLIAKLKES
                     GLQLSAVALQNDQNIARVANVVYNLLPGMVRIFVSYDTVENFLLNNRQWLINKLV"
     gene            2250..>2501
                     /locus_tag="PWY43_002401"
     CDS             2250..>2501
                     /locus_tag="PWY43_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137227.1"
                     /translation="MAGLLPPYFKKKNSDHYKDAVKQFGKEQVDEVIAQCDELESWVK
                     EMPTKQLIMYVGCFHTFNKLSQSKAYVQENFPNVLENPFL"
BASE COUNT          877 a          338 c          455 g          831 t
ORIGIN      
        1 ttgtattcct cacaaaaaaa ttcgccttat tttacttgaa tttacagcca aaatataggg
       61 tggttttggc ttttggtaaa gtaacctttt ccaatagaga tgttatttga ggctgcatag
      121 tactatgcat ttctcttacc ttgacaggta agagaaacaa atcctgaagg aaaaaaattt
      181 taaggaagtt attctatgca atatgtatgt ccaaaaacac tcaaattatt caacagcatt
      241 gttggttgtt ataagggaga agattaattc tctacctcat caaggcttga ttttagattt
      301 aatatccatt tctaaagatg atttagatga tattttggag ggtaatactg atatgaatat
      361 tcatcaatta ttaagcctga tttataaggc tggatttttt gatgtattaa acatcacaaa
      421 taatctatgt ataaaactca gtcaatttgg tttttatttc caaaatggca atttgagcca
      481 aaaagatgat gagcttttga gtttatatag agaattctat aaacataacg agtttatcac
      541 tcatctcaag tcccatagtc tttatggatg tattgagcaa cctacttcgg cattttttca
      601 ttattgtact aatgaaaact ttagaaatgc gtttaatgaa gagagcctct atgattttca
      661 aatgtggaat gtgtttagat aggagttttt tatgtcatct caaaatagtt tcttaacaaa
      721 atattcaaaa agtaattctc atagtcagga ttctcaaaaa ataaatgagt cattagaatg
      781 gctgaatgaa aaaaattatg gtgaattata tagttccttt ctgattgcag tgggagtgaa
      841 agagttttta gataaaggat ttatacctaa tgataattat agagatatta ttgctctact
      901 cccagtagcg ttatatgtct ttcacttaag atctttgaaa catatcaaaa atctagaatc
      961 taacagttat ttagatgaag aaggaagaga tacggtaaat aaatacaagg catttttctc
     1021 tatttttgaa gcagagattc gagatgcaac atgtatttgt gacaataaaa ttgtaacaat
     1081 taatggtgag gtgtgctgtg atattaagga ggtagcaagg aaaattgtta tagaatttct
     1141 aaatgaagtg gatattggta ataaagttaa gttttattac ccagatgaaa gacatgagct
     1201 ttttgtagag ctgctcattg ttctagcaac attaagaata tatcgtaata gaaatatatt
     1261 cttctttgaa aaggggtttt ttaacttttt tcccgaattt atcactattc tcgggactgg
     1321 tgcgtttaat ggatttttag acttccctag tttggtagga aaggtttttc cagaaattat
     1381 tgatacgata aatgagggtt tagatttaga tattgcgatt gagaactttg aagtgttgag
     1441 tcaggaagaa atgactgaat gtatgggttt atatgagcag gctctcatta agaaagctga
     1501 agaaggcgaa attggtttat taaatgttga tgaaataaat agcttttatg aagataatag
     1561 tgagctaatc ttacttgcta aacaacctat ggttgaagag ataatcccta aaccagatag
     1621 caaagcctcc tctgcaattc taaaattctt tgattcaaac tcaaatacct taattgctaa
     1681 attaaaagaa agtggtttgc aattaagtgc tgtggcatta caaaacgacc aaaatatcgc
     1741 acgagtagca aatgtcgttt ataacttatt gccgggcatg gtaagaattt tcgtaagtta
     1801 cgataccgta gaaaatttct tattaaataa ccgccaatgg ttgattaata aattagttta
     1861 atttggaggg gaagaactat ggataaacaa tataatttca ctattcacta ttcactattc
     1921 actatacaga taccagatat aataagaatg ggcaaggtgc tgtttctgaa agaaatatta
     1981 tagatgctaa aaatcgtttt aaagcgagcc atccatacgc aaaaattatt tcttgtgtta
     2041 gagggtctga gacatatgta gcacgtccag caaaagctaa tgctacaaca aaatctgtca
     2101 aggaaaagca atctcaacca caagaaagtt catcgctcag ttcaatgtta cttggtgcgg
     2161 ctgtaactgc tggggttggt tttgtagcca ataaatggtt tagcaaaaaa gataacactt
     2221 aatttatctc taaccaatgt ggagtaaaaa tggctggatt attaccacct tatttcaaaa
     2281 agaaaaatag tgatcactat aaagatgctg taaaacaatt tgggaaagaa caagttgatg
     2341 aggttatagc acagtgtgat gaacttgaat cttgggtaaa agaaatgcca acaaaacaac
     2401 ttattatgta tgttggttgt ttccatactt ttaacaaatt atctcaatct aaagcttatg
     2461 ttcaagagaa ctttccaaac gtactggaaa acccgtttct t
//
LOCUS       DANKBV010000203         2475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.203, whole genome shotgun sequence.
ACCESSION   DANKBV010000203 DANKBV010000000
VERSION     DANKBV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.203"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..417
                     /locus_tag="PWY43_002402"
     CDS             <1..417
                     /locus_tag="PWY43_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248205.1"
                     /note="involved in resistance to antimibial peptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HDL2137228.1"
                     /translation="DSYNVLYCGQTVEIADKESILESSYHPYTRVLLNSSPDFSQPLA
                     FKSSLNTLRGSVPMLQEMPIGCRLGPRCPFAQKKCINKPPLVKVKQHEFACHFPINFR
                     EQNLLKNKTENTPFVVAEIAENTPFVVAEIAEKEAF"
     gene            complement(426..656)
                     /locus_tag="PWY43_002403"
     CDS             complement(426..656)
                     /locus_tag="PWY43_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDL2137229.1"
                     /translation="MKLTACKLVFLLYLMFNLTACGTVLSLVEQDYRVYAGVIKDFQV
                     MQEGGIFAVLAVIDLPLSFVLDTLMLPVTLSQ"
     gene            786..1868
                     /gene="leuB"
                     /locus_tag="PWY43_002404"
     CDS             786..1868
                     /gene="leuB"
                     /locus_tag="PWY43_002404"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDL2137230.1"
                     /translation="MQTYNIAVLAGDGIGPEVMAEAIKVLEATQQKFGFKLNFNQYNI
                     GGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKWTHLPPDEQPERGSLLPLRKHFS
                     LFCNLRPAALYQGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGRDGEGAQTRA
                     FDTEVYYKYEIERITRVAFESAMKRKKHVTSVDKANVLQSSILWRETVTEVAKEFPEV
                     TLEHMYIDNATMQLIKAPESFDILLCSNIFGDIISDEAAMITGSMGMLPSASLNEQGF
                     GLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLGDAADAIETAIQKALADGYR
                     TADLADDSKPLSTSEMGDIIAKNILA"
     gene            complement(1955..2350)
                     /gene="msrB"
                     /locus_tag="PWY43_002405"
     CDS             complement(1955..2350)
                     /gene="msrB"
                     /locus_tag="PWY43_002405"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2137231.1"
                     /translation="MIKPIHALTEQQVDILINHGTERPFTGKFLNEHRVGIYRCARCH
                     NDLFHSDTKFDAGCGWPSFYQTISESSLRYLDDYSLGRHRIEIRCGNCDSHMGHVFPD
                     GPPPTGLRFCLNSAALNFKWEETGEEIDG"
BASE COUNT          744 a          493 c          532 g          706 t
ORIGIN      
        1 gattcttaca atgtgcttta ttgtggacaa accgttgaaa ttgcggataa agagagcatt
       61 cttgaatcgt cttaccaccc ctatactcga gtgttactca atagttcgcc ggacttttct
      121 cagcctttag cctttaaaag tagcttaaat acgctacgag gctcagtccc gatgttacaa
      181 gaaatgccga ttggttgtcg tttaggccct cgttgccctt ttgcacaaaa aaaatgtatc
      241 aacaaaccgc cattagtgaa agtaaaacag cacgagtttg cctgccattt tccgattaac
      301 tttagagaac aaaatttgct taaaaataaa acggaaaata cgccgtttgt ggtggctgaa
      361 atagcggaaa atacgccgtt tgtggtggct gaaatagcgg aaaaagaggc gttttaatgt
      421 ggttattatt ggctaagtgt aacgggtaac attaaagtat ctaatacgaa actcaagggt
      481 aaatcgatca ccgcaagcac cgcgaatata ccgccttctt gcattacttg gaaatctttt
      541 attacgccgg cataaacacg gtaatcctgt tcaaccaagc ttagtactgt gccacaagcg
      601 gtgaggttaa acatcaaata gagtagaaaa acgagtttac aagcggtcaa tttcatcaaa
      661 aaatctccaa atacgctttt attttacgct atttttatcg ggttggcgta aaattcgttt
      721 taaaaacagc tattttcagg cagaatgtag ccgaatcgac attaaatata ctaagaggat
      781 cttatatgca aacttacaac attgcagtgc ttgccggtga cggcattggg ccggaagtga
      841 tggcagaagc tatcaaagtg cttgaagcga ctcaacaaaa atttggcttt aaactaaatt
      901 ttaatcaata taacattggt ggagcagcga ttgatgctca aggtaaagcg ttacccgaaa
      961 ataccttaaa aggctgtgaa gaggcagatg cgattttgtt tggctcggta ggtggcccga
     1021 aatggacgca tttaccacct gatgagcaac ctgaacgagg ttcgctatta ccactgcgta
     1081 aacatttctc gctattctgt aatttacgcc ctgctgcatt gtatcaaggg ctggaaaaat
     1141 tctgcccatt acgtgccgat attgctgcta aaggttttga tatggtggtg gtgcgtgaat
     1201 taaccggcgg gatttatttt ggacagccga aaggccgtga tggtgaaggt gcgcaaacga
     1261 gagcgtttga caccgaagta tactacaaat atgaaatcga gcgtattacc cgtgtggcgt
     1321 ttgaatctgc aatgaaacgt aaaaaacacg taacctctgt ggataaagcc aatgtgttac
     1381 aaagctcaat tttatggcgt gaaaccgtca cggaagtagc taaagagttc ccagaagtca
     1441 cgcttgagca tatgtatatt gataatgcga caatgcagct aatcaaagca cctgaatcgt
     1501 tcgatatttt attatgctca aacatcttcg gcgatattat ttccgatgag gctgcaatga
     1561 ttacaggttc tatgggcatg ttgccttcag ccagcctcaa tgagcaaggt ttcggcttat
     1621 atgaaccggc aggcggttca gcaccggata ttgccggtaa aaatattgcg aatccgattg
     1681 cgcaaatttt atcagccgca atgatgttgc gttatagctt taacttaggt gatgctgctg
     1741 atgcgattga aacagcgatc caaaaagccc ttgctgatgg ctaccgtacc gctgatttag
     1801 ctgatgacag caaaccgctt tctacctcag aaatgggcga tattattgcg aaaaatattc
     1861 tcgcttaagg ctagaagtta aatagcacaa gcggtcatta ttcgccaaat ttttgcaaat
     1921 tttccgctaa taatgaccgc ttgtttatta ctcgttagcc gtcaatctct tcgcctgttt
     1981 cttcccactt aaaattcaat gccgcagagt tgaggcaaaa acgcaaccct gttggtggtg
     2041 ggccgtccgg aaaaacgtgt cccatatgcg aatcacaatt cccacaacga atttcgatac
     2101 gatgacgtcc taagctgtaa tcatctaagt aacgtagtga cgattctgaa atcgtttggt
     2161 aaaaacttgg ccagccacaa ccggcatcaa atttagtatc tgagtgaaat aaatcgttat
     2221 ggcatctggc acaacgataa attcctaccc gatgttcatt caaaaatttt ccggtaaacg
     2281 gacgttccgt accgtggttg attaaaatat caacttgttg ttctgtcagg gcgtgaatag
     2341 gtttaatcat cagcaattct cctaagatag tagcgaagtc attgtagctt tttttgacct
     2401 gaaaatcgtt ataaatttca aaatttgaca tagatcacaa aaaatttatc tgttatattg
     2461 gaagggttct tttgg
//
LOCUS       DANKBV010000204         2442 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.204, whole genome shotgun sequence.
ACCESSION   DANKBV010000204 DANKBV010000000
VERSION     DANKBV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.204"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..910
                     /locus_tag="PWY43_002406"
     CDS             <1..910
                     /locus_tag="PWY43_002406"
                     /inference="COORDINATES: protein motif:HMM:NF019151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2137232.1"
                     /translation="KLNDKEMISEFRSLEKDEIIEFSLCLLRCRLLFDRYIIKTKDDE
                     DDWLLLGLKKYDDGNFKEISSFSRAFRKDDGSDNDEPASDVKEYKLDNYYQYIIHILS
                     MFHTSFRSKIYKNWLFDVLYKISEHPSIKGSYNLKDDVYLTILEEIADKHYKKNKSDA
                     PNFFENGQSTPHYIFNYLDYLLWKNWDKYLDKKENIFIEKNQFRFSLSRTSIEHYLAQ
                     NRTSDSIVHNFGNLCLISPHQNSALSDYETTTKRSFYEGASKRFDCMSLKQAIMLSKE
                     NWTEKDIEEHCEDMKKLLDTKPSQNK"
     gene            complement(1053..1235)
                     /locus_tag="PWY43_002407"
     CDS             complement(1053..1235)
                     /locus_tag="PWY43_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137233.1"
                     /translation="MYQFGYLRTKIGTDTQRAMLWNCPTYVLIIPFLLNTSVKKQYHA
                     FWQKYHQHNNDVSFPY"
     gene            1385..2425
                     /gene="selD"
                     /locus_tag="PWY43_002408"
     CDS             1385..2425
                     /gene="selD"
                     /locus_tag="PWY43_002408"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1430..1432,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDL2137234.1"
                     /translation="MSEPIKLTQFSHGAGUGCKISPKVLGTILQTQLEQFVDPNLLVG
                     NETADDAAVYDLGNGQAIISTTDFFMPIVDAPFDFGRIAAANAISDIYAMGGKPIMAI
                     AILGFPVNKLPAEVAQQIVEGGRFACQQAGIALAGGHSIDSPDPIFGLAVTGIVPTER
                     VKRNASAVAGCELFLTKPLGIGILTTAEKKGLLKPEHQHLASEVMCQINLIGSAFSEL
                     PEVTAMTDVTGFGLLGHLSEVCQGSGLRAEVDFEDIQVLDGTKEYIALGAVPGGTNRN
                     FDSYGHLISAMTDEQKAILCDPQTSGGLLVAVLPQAVEKVQQIANRANVPLFHVGRLL
                     EMEQGKPRIEVI"
BASE COUNT          756 a          402 c          551 g          733 t
ORIGIN      
        1 aaagcttaat gacaaagaga tgataagtga gtttagaagt ctagagaaag atgaaataat
       61 tgaattttct ttatgcttgc taagatgtcg tttgctcttt gatagatata tcatcaagac
      121 taaggatgat gaagatgatt ggctattatt aggtttaaaa aaatatgatg atgggaactt
      181 taaagaaata agttctttca gtagagcatt tagaaaagat gatggttcgg ataatgatga
      241 acctgcaagt gatgtaaaag aatacaagtt agataattat tatcagtata tcattcatat
      301 tttgtctatg tttcatactt cgttcaggtc aaaaatttat aaaaattggc tttttgatgt
      361 gttgtataag attagtgaac atccgtccat taaaggtagt tataacttaa aggatgatgt
      421 gtatttaact attttagagg aaatagctga taaacattat aaaaagaata aatctgatgc
      481 tcctaatttt tttgagaatg gtcaatcaac tcctcactat atttttaatt atttagatta
      541 tcttttatgg aaaaattggg ataaatatct agataagaag gagaatatat ttatagaaaa
      601 gaatcagttt agattttctt tgtcacgaac atcaatagaa cattaccttg ctcaaaatag
      661 aacaagtgat tcaatagttc acaattttgg taatctttgt ttgatttctc cacatcagaa
      721 ttcagcatta agtgattatg aaacgactac aaagagaagt ttttatgagg gagcaagtaa
      781 acgtttcgat tgtatgagtt taaaacaagc tattatgctt tcaaaagaaa actggacaga
      841 gaaggatata gaagaacatt gcgaggatat gaaaaaactt ttagatacta agccttctca
      901 gaataaataa attttcacaa gcggtgcaac tttgcaaaaa cttcggtatt atgtagcagt
      961 tgcacaaaac cccaaattca ttaaaaccgc ttaaaaatga aatgccctaa actgattttt
     1021 cagtctaggg catttattat cacatattat ggttaatagg gaaaactcac atcattgtta
     1081 tgctggtgat atttttgcca aaatgcgtga tattgttttt tcaccgatgt gtttaataaa
     1141 aacgggatta tcaagacata ggtcgggcaa ttccatagca ttgcacgttg tgtatccgtg
     1201 ccgattttgg tgcgtaagta accaaactga tacattagac ccaatccaac cgtcacccat
     1261 tctaatagca taataactaa ggtcgcatca ttaaattaaa agctcatttt ttgcaaaaaa
     1321 tttcccaaat tccaccgctt gttggcgtac aataggcata attttgattt acgatggagg
     1381 cagaatgtct gaaccgataa aacttacgca gttcagccac ggggcgggtt gaggctgtaa
     1441 aatttcgcct aaggtgttag ggacaatttt acaaactcaa ttagaacaat ttgtcgatcc
     1501 gaatttattg gtaggcaatg aaacggcgga cgatgctgcc gtctatgatt tagggaacgg
     1561 gcaggccatt atcagcacca ccgatttctt tatgccgatt gtggacgccc cgtttgattt
     1621 cgggcgaatt gctgctgcca atgcgattag cgatatttat gcgatgggtg gcaagccgat
     1681 tatggcgatt gcgattctgg gctttccggt gaataaattg ccggctgaag tggctcaaca
     1741 aattgtcgaa ggtgggcgtt ttgcgtgcca gcaagccggt attgccttgg cgggcgggca
     1801 ttcgattgat tcgcccgatc cgattttcgg cttggctgtt accggcattg tgccgaccga
     1861 acgggtgaaa cgcaatgcct ctgcggtggc tgggtgtgag ctgtttttaa ccaagccttt
     1921 ggggattggc attctaacca cggcagagaa aaaaggtttg ttgaagcctg aacatcaaca
     1981 tctggcaagc gaggtaatgt gccaaatcaa cttgattggt tcagcgtttt ccgaactgcc
     2041 tgaagtcacg gcaatgactg atgtaaccgg ttttggctta ctcgggcatt taagcgaggt
     2101 gtgtcaaggc tcgggcttac gggcggaggt tgattttgag gatattcaag tccttgatgg
     2161 cactaaggag tatattgcat tgggagcagt tccgggtggg acaaatcgca atttcgacag
     2221 ctatgggcat ttgatttcgg caatgacgga cgagcaaaaa gcgattttgt gcgatccgca
     2281 aacctctggc ggtttattag tggcggtgct gccacaagcg gtcgaaaaag tgcaacaaat
     2341 tgcaaatcgg gcaaatgtgc cgttattcca cgttggcaga ttgctggaaa tggaacaggg
     2401 caaaccacgg attgaggtaa tttgatgttt aaaccaaatg ta
//
LOCUS       DANKBV010000205         2418 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.206, whole genome shotgun sequence.
ACCESSION   DANKBV010000205 DANKBV010000000
VERSION     DANKBV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.206"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            42..503
                     /locus_tag="PWY43_002409"
     CDS             42..503
                     /locus_tag="PWY43_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL2137235.1"
                     /translation="MAKYQMMKLPGGVLSPASEADEESLKALKNHELYDVEINLKINP
                     VLHRKMFSFFKFCFEHWCAENGLQKFSNEKTQFDEFRKDLLVQAGHCEMVFSLSNPNE
                     FKLVPKSISYKELEDDVKRRELYVAITNAAMATVFKGCNDENIINQLYSYF"
     gene            576..1145
                     /locus_tag="PWY43_002410"
     CDS             576..1145
                     /locus_tag="PWY43_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDL2137236.1"
                     /translation="MANKPPKQHKCKECGGYYIKFQSTQQVCSVKCAMAMGKRKTETK
                     RKQADKAERKERKQRLEKLKSRSAWLKDLQNIFNKFIRLRDKDLPCISCGRHHQGQWH
                     AGHYKTVGGNPELRFNEDNVHRQCSVCNNHLHGNIVNYRVNLIEKIGLERVEFLERKD
                     HPPLKLTIEQIKDLIKVYKAKCKELERVT"
     gene            1135..1608
                     /locus_tag="PWY43_002411"
     CDS             1135..1608
                     /locus_tag="PWY43_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137237.1"
                     /translation="MLPRKIKNKWLEPEKQKWIEELLRLWGAWEFGGLDLESRVNMIY
                     RLMKSAEGVKSLSSREVCNDSLGTLINEIFTVVTKRDVMLADILKQKYWFGRSERQIA
                     IYYQLRDSEGRKERRWKEIIQEKCKKAERIIADILESKISSSHSANKLKKYQFNP"
     gene            1744..2103
                     /locus_tag="PWY43_002412"
     CDS             1744..2103
                     /locus_tag="PWY43_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDL2137238.1"
                     /translation="MKMPDKDPNVWLVISAYIQQNYNAITGFVMAFFMSMLRAWFLQQ
                     KSTYRQRFLDGAICGALTLSCMSLLTHFGVGESLSTFAGGMIGFVGAEKIREFLFALI
                     RKKIEVNDVGFNRKSDE"
     gene            2118..>2418
                     /locus_tag="PWY43_002413"
     CDS             2118..>2418
                     /locus_tag="PWY43_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDL2137239.1"
                     /translation="MLISERKFKQVFPNAIDGIYAGIAKHIEKAGCKTKEQQAMFLAQ
                     CGVECAGYKTFEENLNYSATALLATFPKYFTQHNVVLYARDKRAIANRVYANRMGN"
BASE COUNT          800 a          363 c          573 g          682 t
ORIGIN      
        1 ctgggcggtt atgaaggata acgaaggctg ggatggttta gatggctaaa tatcaaatga
       61 tgaaattgcc gggtggagtg ttatctccgg caagtgaagc ggacgaagag agcttaaaag
      121 cacttaaaaa tcacgagctt tatgatgtag aaattaactt aaaaatcaat ccggtgctac
      181 accgcaagat gttctctttt ttcaaatttt gttttgaaca ctggtgtgca gaaaacggtt
      241 tgcagaaatt ctcgaatgaa aagacacagt ttgatgagtt tcgtaaggat ttactggttc
      301 aagctggaca ttgtgaaatg gtgttcagtt tgagtaatcc aaatgaattt aaactagtac
      361 cgaagtcaat ttcttataaa gaattagaag atgacgtgaa acgtcgtgag ttgtacgtgg
      421 caattactaa tgccgcaatg gcaacagtgt ttaaaggatg taatgatgaa aacattatca
      481 atcagcttta ttcgtatttt taatttggtg taattacagg aaattaaaat gagtttttat
      541 tgagtggttt gataaaagag acaaaggagg ttggaatggc taataagcca cctaagcaac
      601 acaaatgcaa agagtgcggc ggttattaca tcaaattcca aagcacacag caggtctgtt
      661 cggtaaagtg tgcaatggca atgggtaaac gtaagacaga gactaaacgt aaacaggcgg
      721 ataaggcaga gcgtaaagag cgcaagcaga gattagaaaa actgaaaagc cgttctgctt
      781 ggttgaaaga cttacaaaat atttttaata agtttatccg tttaagagat aaagatttac
      841 cttgtatttc gtgtggtcgc catcatcaag ggcaatggca cgcagggcat tataaaacgg
      901 tcggtggcaa tccggaattg cgatttaatg aggataacgt acatcgccaa tgctctgtgt
      961 gtaataacca tctacacggc aatattgtga attatcgagt aaatctgatt gagaaaatcg
     1021 ggcttgagcg tgtggaattt ttagagcgta aagaccaccc accattaaaa cttactatcg
     1081 aacaaatcaa agatttaatc aaagtctata aagcgaagtg caaggagctt gagcgtgtta
     1141 cctagaaaaa ttaaaaacaa gtggttagag cctgaaaagc agaaatggat cgaagagttg
     1201 ctaaggttat ggggggcgtg ggaatttggc ggattggatt tagaaagtcg ggtaaatatg
     1261 atttaccggc tgatgaaatc tgcggagggg gttaaaagtc tttcatctag agaggtttgt
     1321 aacgatagcc tcggcacgct gatcaatgag atttttacgg tagtcaccaa aagagacgta
     1381 atgttagctg atatactcaa acaaaaatac tggttcgggc gttcggagcg gcaaatcgct
     1441 atttattatc aactaaggga cagcgagggt agaaaagaaa gacgttggaa agaaattatt
     1501 caagaaaaat gcaaaaaagc ggaaagaatt attgcagata ttcttgaaag caagatttca
     1561 tctagtcatt ctgcgaataa attaaaaaaa tatcaattta acccttgatt ttagtgccca
     1621 ttagatatat attttgtgct aatggtggac gtagtataaa tgatgtttac caggttagaa
     1681 ttttacagcc ctgagcagaa atgtttgggg cttttttatt accccgagat agtggggtgg
     1741 agtatgaaga tgccggataa agatccgaat gtatggctgg taattagtgc atacattcag
     1801 caaaattata atgccattac aggatttgta atggcttttt ttatgtctat gctcagggcg
     1861 tggtttttac aacaaaaaag cacttatcgc cagcgatttc ttgatggtgc aatttgtgga
     1921 gcgttaacgc tttcttgtat gtcgctatta acccactttg gcgttggcga aagtttatct
     1981 acctttgcag gtggaatgat tgggtttgtg ggagctgaga aaatccgtga atttttattt
     2041 gccttaattc gtaaaaaaat cgaagtaaat gatgtcggct ttaaccgaaa aagtgatgaa
     2101 taagtaacgg tggatatatg ttaattagcg aaagaaaatt taaacaagtg ttccccaatg
     2161 cgattgacgg aatttatgcc ggaattgcca aacacattga aaaagccggt tgcaagacga
     2221 aagagcagca agcaatgttt ttggctcaat gtggcgtaga gtgtgccggt tataaaacct
     2281 ttgaagagaa tttgaattat tccgctacgg cattattagc cacatttccg aaatatttta
     2341 cccaacacaa tgtcgtactt tatgctcgag ataagcgtgc tattgctaac cgagtatatg
     2401 cgaaccgtat gggaaacg
//
LOCUS       DANKBV010000206         2286 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.207, whole genome shotgun sequence.
ACCESSION   DANKBV010000206 DANKBV010000000
VERSION     DANKBV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.207"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(36..470)
                     /locus_tag="PWY43_002414"
     CDS             complement(36..470)
                     /locus_tag="PWY43_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137240.1"
                     /translation="MKTTQHILDEREQQHGNYNSFAKIYGGLRKVSDPHAEKLTWRQQ
                     ISVEMILFKLARILNNGSNHQDSWQDIAGYALLGGDIYTPQSSDNTNTKGLPKPLTDS
                     IYPESHLDKNAVWRLDLEFETKEQAVAVLEAVTGKKYSENIT"
     gene            complement(564..1367)
                     /locus_tag="PWY43_002415"
     CDS             complement(564..1367)
                     /locus_tag="PWY43_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HDL2137241.1"
                     /translation="MDKTTLQQISALAVAATKELETDFGAVMLPEGIKLQNLEPFQAH
                     RNQFRAAFSTQRFGSLIEYATANAQENAQCFIDQEKMSAEIVFDMGNREQAGHAKHRA
                     NLVMKKTAAYKALCEINGSRRSQRDFSDFLEDWGIYLNAYHHEDEISIKNAVQAVRKM
                     TIDHARNEEHELSDFAAKKSAMESVEAKSTLQLPTHLVFTCNPYNGLDTRSFTLRVQV
                     LTGSGEPVLTARLVHAEQIEESIATEFAEKLADALSETSIKVNIGTIAI"
     gene            complement(1439..1798)
                     /locus_tag="PWY43_002416"
     CDS             complement(1439..1798)
                     /locus_tag="PWY43_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137242.1"
                     /translation="MAKTNVPEFLDELDCGIFKDKLATALSDVALGVLTHDKKGKVTV
                     EFSLDKMDSDSPSVQIQHKLSYVKPTKRGKSAEEDTTATPMYVHKGGALSATPEKTET
                     AVKESPSGLKALSKAAA"
     gene            complement(1834..>2286)
                     /gene="ssb"
                     /locus_tag="PWY43_002417"
     CDS             complement(1834..>2286)
                     /gene="ssb"
                     /locus_tag="PWY43_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2137243.1"
                     /translation="GINKVIIVGRLGNDPELRTMPNGDSVAKISVATSTEWTDKVSGD
                     KKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIADQ
                     LQMLGSSNSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIPF"
BASE COUNT          550 a          478 c          483 g          775 t
ORIGIN      
        1 actgaaaaat atttttcttt gctcataaat tccccctaag ttatattttc actatatttc
       61 ttgcctgtta cggcttccaa taccgccacc gcttgctctt tcgtttcaaa ctccaaatcc
      121 agccgccata ctgcattttt atcaagatgt gattcgggat aaatgctatc tgttaatggt
      181 tttggtaagc ctttggtatt ggtattgtct gatgattgcg gagtataaat atcgccgcct
      241 aatagggcat atccggcaat atcttgccag ctatcttggt ggtttgagcc gttattcaaa
      301 atacgagcca gtttaaacag tatcatttca accgagattt gctgccgcca agtaagtttc
      361 tctgcgtgcg ggtcgcttac tttgcgtaaa ccaccataaa tcttggcgaa actgttgtag
      421 ttgccgtgtt gttgttcacg ttcatctaaa atatgttggg ttgttttcat cgtgaatttt
      481 cctcgagaaa taaccgcttg tattcaacaa gcggtcaatt ttcattaaaa acttacaagt
      541 aaacttataa gctaacttac aagctagatc gcaatcgtac cgatattgac cttgattgat
      601 gtttcgctaa gtgcatcggc tagcttttcc gcaaactcag tcgcaataga ctcttcaatt
      661 tgctcagcat gaactaagcg ggcagttagt acaggttcac cgctaccggt tagcacctgt
      721 acacgcagag taaatgatcg agtgtctaag ccattgtatg gattgcaagt aaatacaagg
      781 tgagttggca gttgcagtgt agatttagct tctaccgatt ccatcgctga ttttttggct
      841 gcgaaatcgc tgagttcatg ttcttcattt cgggcgtggt caatcgtcat tttacgcacc
      901 gcttggacgg catttttaat actgatttca tcttcgtggt ggtaagcgtt caagtagatg
      961 ccccaatctt cgaggaaatc agaaaaatca cgctgtgagc gacgtgagcc attaatttcg
     1021 catagagctt tgtatgctgc ggttttcttc atcaccaaat tagcacgatg tttagcgtga
     1081 cctgcttgtt ctcggttgcc catatcaaat acaatttccg cactcatttt ctcttggtcg
     1141 ataaagcatt gagcattttc ttgagcattg gcggtagcat attcaatcag gctaccaaaa
     1201 cgttgcgttg aaaacgctgc acggaattgg ttacggtgag cttggaaagg ctctaaattt
     1261 tgtaatttaa tgccttctgg tagcattact gcaccaaaat cagtttcaag ttctttggtt
     1321 gcagcaacag caagagccga aatttgttga agtgtcgttt tgtccatttt aagttccttt
     1381 ttgtttgagt aaaaaaataa agccacgcat taacgtggct tggattgaaa ataattgatt
     1441 atgcagcagc tttagataga gcctttaaac cgctagggct ttcttttact gccgtttctg
     1501 ttttttcagg tgtagcagat aatgccccac ctttatgaac atacattggc gtggcggttg
     1561 tatcctcttc tgctgattta ccacgcttgg tcggtttgac atagctcaat ttgtgctgaa
     1621 tttgaacaga tgggctgtcg ctatccattt tgtctaagct aaattcaacc gttaccttac
     1681 cctttttatc gtgggttagt acacctaacg ccacatctga aagagctgtt gcgagcttgt
     1741 ctttgaaaat accgcaatct aattcatcta aaaattcagg tacgtttgtt tttgccattt
     1801 ttggttctcc taattagggt ttgaataaaa ttattagaac ggaatatcat catcaaaatc
     1861 tcgtgattct gaatccgtca ttacgtggtt atatggattg gttggcggtg ggtttcctgc
     1921 cggttcggtt gcccaattat tattgctgtt tccgctgttt gagctaccga gcatttgtag
     1981 ctgatcggcg atgatttcgg tcgtccagcg gtctgtgccg tcttgggctt gccatttgcg
     2041 agtgcgtagt ttgccctcaa catagacttg tgagcctttt ttcaggtatt tgccaataat
     2101 gtcagctaaa ttccgaaagg cgataatgct atgccattct gtggcttgtt ttttatcgcc
     2161 tgatacttta tccgtccatt cggtcgaagt tgccacactg attttggcga ctgaatcgcc
     2221 atttggcatt gttcgtagtt ccgggtcgtt acctaggcga ccgacaataa tcactttgtt
     2281 aattcc
//
LOCUS       DANKBV010000207         2281 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.208, whole genome shotgun sequence.
ACCESSION   DANKBV010000207 DANKBV010000000
VERSION     DANKBV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.208"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..88)
                     /gene="rrf"
                     /locus_tag="PWY43_002418"
     rRNA            complement(<1..88)
                     /gene="rrf"
                     /locus_tag="PWY43_002418"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(250..>2281)
                     /locus_tag="PWY43_002419"
     rRNA            complement(250..>2281)
                     /locus_tag="PWY43_002419"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          514 a          665 c          457 g          645 t
ORIGIN      
        1 gaatcaccac actaccatcg acgttactgc gttttacttc tgagttcggt atggggtcag
       61 gtaggaccac agcactcttg tcgtcgggaa aattcttttc tatttttgaa tttctttaag
      121 ttaacttcct aatctaactc ttagttcact ttaatagtta gattaacttc cttaacttca
      181 attgatttct tctctagttt cgttcttctt ctcaatacaa aaacaagcct aaaaacattt
      241 gagcgttgta tagttaagcc tctcgggcaa ttagtactgg ttagctcaat gtctcacaac
      301 acttacacac ccagcctatc tacgtcgtag tctccaacaa cccttacaca cttatagtgt
      361 gggagaactc atctcgaggc aagtttcgtg cttagatgct ttcagcactt atctcttccg
      421 catgtagcta cccagcaatg cctctggcga gacaactgga acaccagtga tgcgtccact
      481 ccggtcctct cgtactagga gcagcccctc tcaattctcc aacgcccacg gcagataggg
      541 accgaactgt ctcacgacgt tctaaaccca gctcgcgtac cactttaaat ggcgaacagc
      601 catacccttg ggacctactt cagccccagg atgtgatgag ccgacatcga ggtgccaaac
      661 accgccgtcg atatgaactc ttgggcggta tcagcctgtt atccccggag taccttttat
      721 ccgttgagcg atggcccttc cattcagaac caccggatca ctatgacctg ctttcgcacc
      781 tgctcgactt gtctgtctcg cagttaagct tgcttatacc attgcactaa cctcacgatg
      841 tccgaccgtg attagcaaac cttcgtgctc ctccgttact ctttgggagg agaccgcccc
      901 agtcaaacta cccaccagac actgtccgag accgcgttcc gcaatcttcg ttagaacatc
      961 aaacgttaaa gggtggtatt tcaaggacgc ctccacaatc actggcgtga ctgcttcaaa
     1021 ggctcccacc tatcctacac atcaaaattc aatgttcagt gtcaagctat agtaaaggtt
     1081 cacggggtct ttccgtctag ccgcgggtac accgcatctt cacggcgatt tcaatttcac
     1141 tgagtctcgg gtggagacag cctggccatc attatgccat tcgtgcaggt cggaacttac
     1201 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
     1261 tcgatcagga gcttctcttg cgataacacc atcaattaac cttccagcac cgggcaggca
     1321 tcacacccta tacgtccact ttcgtgtttg cagagtgctg tgtttttaat aaacagttgc
     1381 agccagctgg tatcttcgac cggttcaacc ttcatccgca agggactaca atctacgccg
     1441 gcgcaccttc tcccgaagtt acggtgctat tttgcctagt tccttcaccc gagttctctc
     1501 aagcgcctga gtattctcta cctgaccacc tgtgtcggtt tatagtacgg tttataataa
     1561 cctgaagctt agtggctttt cctggaagcg tggtatcggt tacttcatct ccgtagagac
     1621 tcgtcatcac ttctcggtgt taaggaagac cggatttgcc taatcttccc acctaccggc
     1681 ttaaacagac atccaacagt ctgctaacct aaccttctcc gtccccacat cgcagttatt
     1741 acaagtacgg gaatattaac ccgtttccca tcgactacgc ttttcagcct cgccttaggg
     1801 gccgactcac cctgccccga ttaacgttgg acaggaaccc ttggtcttcc ggcgaacgag
     1861 tttttcactc gttttatcgt tacttatgtc agcattcgca cttgtgatat ctccagcaga
     1921 cttctcaatc caccttcatc gacttacaca acgctcccct acccaacaga ctttcgtctg
     1981 atgccgcagc ttcggtgcta tatttgagcc ccgttacatc ttccgcgcag gccgactcga
     2041 ctagtgagct attacgcttt ctttaaatgg tggctgcttc taagccaaca tcctagctgt
     2101 ctaagccttc ccacttcgtt tcccacttaa tatagacttt gggaccttag ctggcggtct
     2161 gggttgtttc cctctccacg atggacgtta gcacccaccg tgtgtctcct gagtatcact
     2221 cttcggtatt cgcagtttgc atcgggttgg taatccggga tggaccccta gccgaaacag
     2281 t
//
LOCUS       DANKBV010000208         2276 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.209, whole genome shotgun sequence.
ACCESSION   DANKBV010000208 DANKBV010000000
VERSION     DANKBV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.209"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(8..979)
                     /gene="menC"
                     /locus_tag="PWY43_002420"
     CDS             complement(8..979)
                     /gene="menC"
                     /locus_tag="PWY43_002420"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDL2137244.1"
                     /translation="MRQVKLYRYQLPIQTGVVLRKQPLKERQGVLVCLQEQEKVGWGE
                     IAPLPSFSQESLEQAENQAVNWLKAWQKGEPETLEQLFPSVAFGLSCALAELNNELGE
                     QANNQSAVLCYGDLEKLHTAFSQHTNPIGKIKIGLNPEQEGEQANLLLHTFPNLRLRL
                     DANRLWSLEQAVKFAEKIANEHKPRIQFIEEPCQALELSRQFATQTDIAIAWDETVRE
                     ANFLVKKEPNLTAIIIKPTLVGSLEKCVNLIQQAQKQGLVAVISSSLESSLGLTQLAR
                     IAQQYTPNSVPGLDTLNLMQHQLLRDWHDSSLPLVGLESEFVREVVV"
     gene            1191..>2276
                     /locus_tag="PWY43_002421"
     CDS             1191..>2276
                     /locus_tag="PWY43_002421"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL2137245.1"
                     /translation="MANQKRKMLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGN
                     EVHFVCADDAHGTPIMLNADKLGITPEQLIEKAKADHEADFASFNISFDNYHSTHSEE
                     NRQITTDIYKTLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
                     VCASTYSPMDLINPRSAVSGATPVVKESEHFFFDLPSFEGMLKEWTRSGSLQPEIANK
                     MQEWFESGLQQWDISRDAPYFGFEIPDAENKFFYVWLDAPIGYMASFKNLCDRAGIDF
                     DEFWQKDSTTELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSK
                     SRGTFIQANTYLKHLDPECLRYYYAAKL"
BASE COUNT          623 a          523 c          470 g          660 t
ORIGIN      
        1 tgggagatta taccacaact tccctaacaa attcgctttc tagcccgacc aacggcaagc
       61 tagagtcgtg ccaatctcgc aataattggt gttgcattaa atttaacgta tccaaccccg
      121 gcacactgtt tggggtgtat tgttgggcga tacgagccag ttgggttaag cccaaactgc
      181 tctcaagact tgaactaatc accgctacta atccttgctt ctgtgcttgt tgaattaaat
      241 tcacgcattt ttcaagcgaa ccaaccagcg taggtttaat gataatagcg gtcaaatttg
      301 gctctttttt taccaaaaaa ttcgcctctc tcacggtttc gtcccacgca atcgcaatgt
      361 cggtttgtgt ggcaaattgg cgggaaagtt cgagcgcttg gcaaggttct tcgataaatt
      421 gaatacgggg tttatgttca tttgcaattt tttcagcaaa tttgaccgct tgttccaagc
      481 tccaaagacg gttggcatca agccttaggc gtaaattcgg aaaagtatgc aacagcaaat
      541 ttgcctgctc gccttcctgt tcaggattga gaccgatttt aattttaccg attgggttcg
      601 tatgttggct aaatgccgtg tgtaactttt ccaaatcgcc atagcacagc accgccgatt
      661 ggttattcgc ttgctcgcct aattcattat tcagttcagc caacgcacag cttagcccaa
      721 aggcaacaga cgggaaaagc tgctctaacg tttcaggctc tcctttctgc cacgccttta
      781 accaattcac agcttggttt tcagcttgtt ctaggctctc ttggctaaaa cttggaagcg
      841 gtgcaatttc gccccagccg actttctcct gttcttgcaa acacaccaac accccttgcc
      901 tttctttcaa aggttgcttg cgtaacacta caccggtttg aatggggagt tggtagcggt
      961 ataactttac ttgtcgcata ctatttttat ccaaaatagg tttagaggcg aaacatcttt
     1021 cgcccacatc atcatttggg aaattgaggc gaaaaatgtt tcgcctctag cgcatcatat
     1081 tctaccgctt gttagcccaa agctctaata aattcacgcc aactggtaaa cctgcaactt
     1141 ttgggctaaa atagacggca atttttaccc taattttaat gagaaaaaga atggctaacc
     1201 aaaaacgaaa aatgcttgtt acttgtgcct tgccctatgc taacggtgca attcatttag
     1261 gtcatatgct tgaacatatt caagcagata tttgggtgcg ttttcaacgt atgcgtggca
     1321 atgaagtcca ctttgtctgt gctgatgatg cccacggcac gcctattatg cttaatgccg
     1381 ataaattagg cattacccct gaacagttaa tcgaaaaagc caaagccgat cacgaagcgg
     1441 attttgccag ttttaatatc agctttgata attaccattc cacccacagc gaagagaacc
     1501 gccaaatcac taccgatatt tataaaacct taaaagcgaa cggttttatt aaaagcaaag
     1561 tgatttcaca actgttcgac ccagaaaaaa atatgttctt gccggatcgc tttgtgaaag
     1621 ggacctgccc gaaatgtaaa gccgaagatc aatatggcga taactgtgaa gtatgtgcct
     1681 ctacctatag cccgatggat ttaatcaacc ctcgctcggc agtttccggt gcaactccgg
     1741 tggtaaaaga atcagaacat ttcttttttg acctgccaag ctttgaggga atgttgaaag
     1801 agtggactcg ttccggctca ctccaacctg aaattgccaa taaaatgcaa gaatggtttg
     1861 aaagtggttt acagcaatgg gacatcagcc gtgatgcccc ttatttcggt tttgaaattc
     1921 cggacgcaga aaacaaattc ttctacgttt ggttagatgc ccctattggt tatatggcat
     1981 cgtttaaaaa tttatgcgac cgtgcaggca ttgattttga tgagttttgg caaaaagatt
     2041 ccaccaccga gctttatcac ttcatcggta aagacattgt ttacttccac agcctgtttt
     2101 ggccggcaat gttggaaggc agcggctacc gcaaaccgac caatgtgttt gctcacggtt
     2161 atgtgacagt ggacggggcg aaaatgtcga aatctcgtgg tacattcatt caagccaaca
     2221 cctatttaaa acatctcgat ccggaatgct tacgctatta ctatgcggca aaatta
//
LOCUS       DANKBV010000209         2248 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.210, whole genome shotgun sequence.
ACCESSION   DANKBV010000209 DANKBV010000000
VERSION     DANKBV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.210"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..>2248)
                     /locus_tag="PWY43_002422"
     CDS             complement(<1..>2248)
                     /locus_tag="PWY43_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137246.1"
                     /translation="NYERIGDYQKANLILKNIHNQDDFVQWLSTEQGKKLYSRVGYDK
                     GVWEALSVLLPERFDFEVFEVIDYSKEWSWFEIIVENLFWRQPEKINMQKLHQFFKNN
                     PYFNQATWINILYQLATEINHPLNIKYLHNKLYSFSLADRDEIWTAFIALESDQCPAI
                     ERLLTWCQKSSGQENIEPEALLLGAIAVSWLFTSTDKRSRNGYINALAHLLWNRLEIA
                     LALFKEFIEVNDPYVMEGILSAIHGAVAYSENSSGLKALALEIDNQIFNQPETEEIYP
                     NVLVRNYARHIIEYSLQKDEYSSDDIEIIRERITPPYRSSFPQNLPTNEEIDKKYHSD
                     GQRIIIRSMTTEYGRGMCGYGDFGRYTFEAKFRNWESYKFGNLINVDLLSNYACQMIF
                     EQFGYDDKKHLEFDKETRSRYWRRDDTNKIERIGKKYQWLALYEILARVMDNFPAFSN
                     RYADEIGLEYITNLDNFYLPKTQIKIVPPTKTDVFFQYINQKQCKTKHYFTIQNDELI
                     DDWVKINDFPDVSNLLEVTINDEQWLVLERHDDFRELAKFGQKETYDRQMWLQIRSYL
                     VKEFQFSKAKNWLAQQDFMERWMPEGNEHYGMNIHQFFAENTLNEWQEIYRHRENESP
                     SFKVIPSVDSHTWESTYGNDGISFYAPIPMLYHGMKMKNAIKDGYWVDLQNNLVCFNP
                     EINEKFQNSLLVKKDKFLDFLKEHKLKLVWTVLGEKHAYKESKFLQELSGVYYLYGKN
                     QQVQGSIKH"
BASE COUNT          727 a          411 c          297 g          813 t
ORIGIN      
        1 aatgtttaat actgccttgg acttgttgat ttttaccata tagataataa acgcctgaaa
       61 gttcttgtaa aaatttactt tctttataag cgtgtttttc acccaacaca gtccaaacta
      121 actttaactt atgctctttt aaaaaatcca aaaatttgtc ttttttaacc agtaagctat
      181 tttgaaattt ttcatttatc tctggattaa agcaaaccaa attattttgt aaatcaaccc
      241 aataaccatc tttaatcgca tttttcattt tcattccgtg ataaagcata ggaattggcg
      301 cataaaatga aatgccgtca ttgccataag tagattccca agtgtgagaa tcgacactag
      361 gtattacctt aaatgaaggc gattcatttt ccctatgtcg ataaatttct tgccactcat
      421 ttaatgtatt ttcggcaaaa aattgatgaa tattcattcc atagtgttca ttaccttctg
      481 gcatccatct ttccataaaa tcttgttgtg ccaaccaatt ttttgctttt gaaaattgaa
      541 attctttaac taaataactt ctaatttgta accacatttg cctatcataa gtttcttttt
      601 gcccaaattt tgctaattca cgaaaatcat cgtgtctttc tagaacaagc cactgttcat
      661 cattaatcgt tacttccaaa agattactaa catcaggaaa atcatttatt tttacccaat
      721 catcaattaa ttcatcattt tgaatggtaa aatagtgttt tgttttacat tgtttttgat
      781 ttatgtattg aaaaaataca tctgttttag ttggcggtac aattttaatt tgggttttgg
      841 gtagataaaa gttgtccaaa ttagtaatat attcaagccc tatttcatcg gcatagcgat
      901 tagaaaaagc aggaaaatta tccatcaccc ttgccaagat ttcatacaaa gccaaccatt
      961 gatatttctt gccaattcgc tcaattttat tagtatcatc tcttcgccaa tatcttgaac
     1021 gagtttcttt atcaaactca aggtgcttct tgtcatcata gccaaattgc tcaaaaatca
     1081 tttggcaagc ataattactt aataaatcaa cattgatgag attgccgaat ttataactct
     1141 cccaatttcg gaatttggct tcaaaagtat atctgccaaa atcaccatat ccacacatac
     1201 cacgaccata ttcagttgtc attgaacgga taatgattct ttgtccatca gaatgatatt
     1261 ttttatcaat ttcttcattg gttggtaagt tttggggaaa tgaacttcta taaggtggcg
     1321 taattctttc tctgattatc tcaatatcat ctgatgaata ttcatctttt tgcaaagagt
     1381 attcaataat atggcgagca taattacgaa ccaaaacatt gggataaatt tcttctgttt
     1441 caggttgatt aaatatttga ttatctatct ctaatgcaag tgcttttaag ccagatgaat
     1501 tttcagaata agcaacagct ccgtgaatgg cagataaaat accttccata acataagggt
     1561 cattaacctc tataaattcc ttgaataacg ccaatgcaat ttccaatcta ttccataata
     1621 aatgagccag tgcattaata tagccatttc tacttcgttt atcggtagaa gtaaatagcc
     1681 aactaacagc aattgcaccc aataatagtg cttcaggttc aatattttct tgaccacttg
     1741 atttctgaca ccaagttagt aatcgttcaa ttgctgggca ttgatcagac tccaaagcaa
     1801 taaaagctgt ccaaatctca tctctatctg ccaaagaaaa agaataaagt ttgttatgta
     1861 aatattttat atttaatggg tggttaattt cggtagccag ttgatataaa atattaatcc
     1921 aagttgcttg attaaaatat ggattatttt tgaaaaattg atgtagtttt tgcatattga
     1981 ttttttcagg ctgcctccaa aagagatttt ctacaataat ctcaaaccaa gaccattcct
     2041 ttgaataatc aatcacttca aatacttcaa aatcaaatcg ttctggcaac aatacactta
     2101 atgcttccca tacgccctta tcatagccaa ctctactata tagctttttt ccttgttcag
     2161 tgcttaacca ttgaacaaaa tcgtcttgat tatgaatatt ttttaatatt aaattggcct
     2221 tttgataatc cccaattcgt tcataatt
//
LOCUS       DANKBV010000210         2237 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.211, whole genome shotgun sequence.
ACCESSION   DANKBV010000210 DANKBV010000000
VERSION     DANKBV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.211"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..169
                     /locus_tag="PWY43_002423"
     CDS             <1..169
                     /locus_tag="PWY43_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL2137247.1"
                     /translation="AVHAGNGYNVCNNGYKMALIYLVMLVPLLLQGAGSLSLDFLLQK
                     KSPTKRWLKFL"
     gene            195..482
                     /locus_tag="PWY43_002424"
     CDS             195..482
                     /locus_tag="PWY43_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137248.1"
                     /translation="MKKATLMALAGALTMATATVNAETTSVKADACMEVKDGKCVKTK
                     ATEGKCGEGKCGATSSEPKATEGKCGEGKCGATAPKAAEGKCGEGKCGASK"
     gene            583..1506
                     /locus_tag="PWY43_002425"
     CDS             583..1506
                     /locus_tag="PWY43_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HDL2137249.1"
                     /translation="MLKFTGAGLGYRRDLADGFLSLTDNNIINFMEVAPENWLKMGGQ
                     ARYNFDKVAEKIPVIIHGLSLSLGGQAPLDMTLLKGIKNMQQQYNSFFFSDHLSYCEC
                     EGHLYDLLPMPFTDEAVHHVANRIKQVQDYLGMQISLENTSYYLHSPTSTMNEVEFLN
                     AIAQEADCGIHLDVNNIYVNGVNHGLLDPFVFIDQVDKSRVNYIHIAGHDEDHGAAED
                     VKMTEGESFNMVKGELRHLPTLLVDTHGEAVQKTVWDLLEYTYEHLPHIPPTLLERDF
                     NFPPFQELISEVQHIMSLQKKYAKTRLKNAA"
     gene            1496..2236
                     /locus_tag="PWY43_002426"
     CDS             1496..2236
                     /locus_tag="PWY43_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HDL2137250.1"
                     /translation="MPLKLVEEHKPLLKEIQAEFANAIRHNDVKLMSGNISPQRLGVY
                     SRLVRNNTLGFIDRCYVQLPQHLSPEEWVAVKEKFIREGKAHSPFFQDIAGEFLNFCR
                     ENGCMPPHLLELMDFENAQLLAEVSMATVPSEFEWDNDTIMQFSGAAELRQYAVNFIR
                     SEFKQFDFEPTNVLIWRDAEFGIYYNRLEELDFFLLSSLQEGANSLNGLLAELSEFTE
                     QNLELKALLRQLWEKWVEAEVIYPVYPE"
BASE COUNT          682 a          449 c          490 g          616 t
ORIGIN      
        1 tgctgttcac gctggcaatg gctacaatgt ctgcaacaat ggctataaaa tggcactcat
       61 ttatttagtg atgttagtac cacttttatt acaaggtgct ggctcgctct ctctcgattt
      121 tctgttacag aaaaaatccc caaccaaacg ctggttgaaa ttcctttaat cccttaattt
      181 atggagaatt tttaatgaaa aaagcaactt taatggcatt agctggtgca ttaactatgg
      241 caaccgcaac tgtaaacgct gaaactacat cagtgaaagc agatgcttgt atggaagtaa
      301 aagatggcaa atgtgtaaaa accaaagcta cagaaggtaa atgtggcgaa ggcaaatgtg
      361 gagcaacctc ttctgagcca aaagcaaccg aaggtaaatg cggcgaaggc aaatgtggag
      421 caacggcacc aaaagcggca gaaggtaaat gcggtgaagg caagtgtgga gcttctaagt
      481 aagtagcgaa ggtgcgtttt ctaaacgcac ctaacctctt ataaacacaa tctacaagcg
      541 gttaaattcc gctgattttt tgcaaattaa aggaacaaga cgatgttaaa atttactggt
      601 gctggtctag gctatcgccg tgacctagcg gacggatttt tatctctcac agataacaat
      661 ataattaatt ttatggaagt agccccagaa aactggctga aaatgggcgg tcaggctcgg
      721 tataacttcg ataaagtcgc tgaaaaaatc cctgttatta ttcacggttt atccctctct
      781 ctcggcgggc aagcgccttt agatatgacg ctactaaaag ggatcaagaa tatgcagcag
      841 cagtataact cctttttctt ctctgatcat ttaagctatt gcgaatgtga agggcattta
      901 tatgacctgc tgccaatgcc gtttaccgat gaagccgtac atcacgtggc aaatcgcatt
      961 aaacaagtgc aagattattt aggtatgcaa atttcccttg aaaatacgtc ctactatctg
     1021 cattcaccaa ccagtacgat gaatgaggta gagtttctca atgcaattgc ccaagaagcg
     1081 gattgtggca ttcaccttga tgtgaataat atttatgtta atggtgtcaa tcacggcttg
     1141 cttgatccat ttgtatttat tgatcaagtg gataaatctc gtgtcaatta tattcatatt
     1201 gcaggccacg atgaagatca cggtgcggcg gaagacgtta aaatgaccga gggtgaatca
     1261 tttaatatgg taaaaggcga actacgtcat ctgccaactt tactggtcga tacccacgga
     1321 gaagctgtac aaaaaactgt gtgggattta ctagagtaca cctatgaaca cctaccgcat
     1381 attccaccaa cactgttaga acgggatttc aacttcccac cgtttcaaga actgattagt
     1441 gaagtacagc acattatgag cttacagaaa aaatatgcaa aaacgaggct aaaaaatgcc
     1501 gcttaaactc gttgaagaac acaaaccgtt actcaaagag attcaagccg agtttgcgaa
     1561 tgcaattcgc cataacgatg taaagctgat gagtggtaat atctctccac agcggcttgg
     1621 cgtctattct cgcttggttc gcaataatac tctcggtttt atcgaccgtt gttatgtaca
     1681 gctccctcaa catctaagtc ctgaagaatg ggttgccgtt aaagaaaaat ttatacgaga
     1741 aggtaaggct cattcgccct ttttccaaga tattgctggc gaatttctca atttttgccg
     1801 agaaaatggt tgtatgccac cacacttgct tgagttgatg gactttgaaa atgctcaatt
     1861 actggctgag gtatcaatgg caaccgtacc atcagaattt gaatgggata acgatactat
     1921 aatgcagttt tctggtgcag ctgagctgcg tcaatatgcg gtaaacttta tccgtagcga
     1981 gttcaaacag tttgactttg agccgaccaa cgtactgatt tggcgagatg cggaatttgg
     2041 catctattac aaccgcttgg aagagctgga tttcttcctg cttagctcgt tgcaagaggg
     2101 ggcaaattcc ctgaatggct tactagcaga actcagcgag tttaccgagc aaaatctgga
     2161 gctaaaagcc ttgttacgcc aactttggga aaaatgggtt gaagcagagg tgatttatcc
     2221 tgtctatcct gaatgag
//
LOCUS       DANKBV010000211         2236 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.212, whole genome shotgun sequence.
ACCESSION   DANKBV010000211 DANKBV010000000
VERSION     DANKBV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.212"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            173..1396
                     /gene="hflK"
                     /locus_tag="PWY43_002427"
     CDS             173..1396
                     /gene="hflK"
                     /locus_tag="PWY43_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDL2137251.1"
                     /translation="MSWNESGNQQDPWGKPGQKKAEPKPEQQEQGSQQEPANNQQNEQ
                     QPPDLEEVFSSLLKKMGGGKGSNNSSNQPTASLGKFLPVVLGLAAIVWAGSGFYTVQE
                     AERGVVTRLGKLDSIVMPGLNWKPTFIDSVTRVNVERVSELNTSGSMLTQDENMVQVE
                     MTVQYRVEDPAKYLFSVSNPDDSLKQATDSALRYVIGHMTMDEILTTGRATVRERTWS
                     TLRDIIKTYDMGLLVTDVNFQYARPPEEVKAAFDDAIKAQEDEQRLIREAEAYARGEE
                     PIARGQAQRTIEQAQAYKEAVVLNAKGEVERLSQLLPEYKASPELTRERLYIQTMEKV
                     MKNTPKVVMDSSGNNLNVLPFDKLMNSSSVIKAEQPAAAQVVTPQVVQPQVQPRTQIQ
                     PQQAEQQEPTRKGRF"
     gene            1399..>2236
                     /gene="hflC"
                     /locus_tag="PWY43_002428"
     CDS             1399..>2236
                     /gene="hflC"
                     /locus_tag="PWY43_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDL2137252.1"
                     /translation="MRRLLVPILAVVAFVVLQAITIVNEGERGIMLRFNKVHRDSDQK
                     VVVYEPGIHFKVPFIDSLKVLDARIQTLDGQEDRFVTVEKKDLLVDSYVKWRISDFGQ
                     FYTSTGGDYQKAADLLRRKVGDRLRSEIGSRTIKDIVSGSRGELMAGAQKALNAGEDG
                     AERLGIEVVDVRVKQINLPNEVSSSIYQRMRAERDAVAREHRSQGNEKAEVIRAEVDK
                     KVVLILANANKTAQALRGEGDAQAAKLYSEKFGNEPEFYSFIRSLKAYEDSFAEGQNN
                     MML"
BASE COUNT          712 a          386 c          547 g          591 t
ORIGIN      
        1 aattttaaga gcaatactag ggcgaattta tcgccctatt tttttgcaaa taaatagtga
       61 aaaaagaccg cttgtttcag ataaacctag tcaaaagctt actaaatagg tacaatcact
      121 aacttgcatt ctaagcttta aagtgcgatt cgattttatt ttggagatat ttatgtcgtg
      181 gaatgaatca ggcaatcagc aagatccttg gggtaaaccg gggcagaaaa aagctgagcc
      241 aaagcctgag caacaagaac agggttcaca acaagagccg gccaataatc aacagaatga
      301 acaacagccc cctgatttag aagaagtatt tagcagttta ttaaaaaaaa tgggtggtgg
      361 aaaagggtct aataatagtt caaatcaacc tactgcaagc ttagggaaat tcctacctgt
      421 tgttttaggt cttgctgcaa tcgtttgggc tggttccggt ttttatactg tacaagaggc
      481 tgagcgtgga gtagttactc gtttagggaa actcgatagt atcgtaatgc ctgggttgaa
      541 ctggaagccg acttttattg attctgtcac cagagtaaat gtggaacgtg tgtcagagct
      601 taatacaagc ggttcaatgc tgacacaaga tgaaaatatg gtgcaggtgg aaatgacggt
      661 tcaatatcgt gtggaagatc cggctaaata tttgtttagt gtaagcaatc ctgatgacag
      721 cttaaaacaa gctacagata gtgcattgcg ttatgtgatt ggtcatatga cgatggacga
      781 aattttaact actgggcgtg caaccgtacg cgaaagaacg tggtccacat tgcgtgatat
      841 tatcaaaact tatgatatgg ggttattggt aacagacgta aacttccaat atgcacgacc
      901 gcctgaagag gtgaaagctg cctttgatga tgcaatcaaa gcacaagaag atgagcaacg
      961 cttaattcgt gaagcggaag cctatgcaag aggtgaagag ccgattgcac gtggccaggc
     1021 acagcgtacg attgagcaag ctcaagctta caaagaagca gttgtgttaa atgcgaaagg
     1081 ggaagtggag cgtttgagtc aattattacc tgaatataaa gcctctccag agctgactcg
     1141 tgaacgttta tatatccaga caatggaaaa agtcatgaaa aatacgccga aagtggtgat
     1201 ggattcaagt ggtaataatt tgaatgtatt gcctttcgat aagctaatga attcttcatc
     1261 ggtaataaaa gcggagcagc ctgcggcggc acaagtagtt acaccacaag ttgttcagcc
     1321 gcaagtacag ccaagaacac aaattcagcc gcaacaagca gaacaacaag aaccaactcg
     1381 taaggggaga ttttaatcat gcgtagatta ttagtaccta tacttgctgt agttgctttt
     1441 gttgtgctac aggcaatcac cattgtaaac gagggcgagc gtggcattat gttacgtttt
     1501 aacaaagttc atcgtgatag cgatcaaaaa gtggtggtat atgaaccggg gattcacttt
     1561 aaagtaccat ttatcgatag tttaaaggta ttagatgctc gtattcaaac tctagatggg
     1621 caagaagacc gttttgtaac agtcgagaaa aaagatttat tagtggactc ctatgtaaaa
     1681 tggcgaatca gtgattttgg ccaattttat acctctactg ggggggatta ccaaaaagcg
     1741 gcggatttat tacgccgtaa ggtaggggat cgtctgcgtt ccgagattgg ttctcgaacc
     1801 attaaagata ttgtatccgg ctctcgtggt gagttaatgg caggagccca aaaagcgctg
     1861 aatgccggtg aagatggagc agagcgttta ggaattgaag tagttgatgt gcgagtgaag
     1921 caaatcaact taccaaatga agtttcttct tcaatttacc aacgtatgcg tgccgagcgt
     1981 gatgcggtgg ctcgtgaaca ccgctcacaa ggtaatgaga aagcagaggt aattcgtgcc
     2041 gaagtggata aaaaagtggt gctgattttg gcaaatgcga acaaaactgc tcaagccttg
     2101 cgaggtgaag gtgatgctca agctgcaaaa ctctattcag agaaatttgg taacgagccg
     2161 gaattttaca gttttattcg tagtttaaaa gcctatgaag atagctttgc cgaaggtcaa
     2221 aataatatga tgttat
//
LOCUS       DANKBV010000212         2234 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.213, whole genome shotgun sequence.
ACCESSION   DANKBV010000212 DANKBV010000000
VERSION     DANKBV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.213"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            15..1028
                     /gene="cas1c"
                     /locus_tag="PWY43_002429"
     CDS             15..1028
                     /gene="cas1c"
                     /locus_tag="PWY43_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HDL2137253.1"
                     /translation="MRKLQNTLYITSQGSYLHKERETLVVEQDRKKVAQLPIHSIGHI
                     FCFGNVLVSPFLMGFCGENNVNLAFFTENGRYLGRLQGRQSGNVLLRRAQYKKSETNP
                     VPVARNIIAAKIQASKRVLQRQIRNHGENADIQLAITALNYSLQQLKIADNLNLIRGI
                     EGDAAARYFGMFSHLIKENSGFHFDGRNRRPPRDGVNALLSFLYSILGKDISGALQGV
                     GLDPQIGFLHADRPGRDSLAQDILEEFRAWWVDRMVLSLINRGQVKPDDFVTEASGAV
                     TLKPEARKLLFQTLQAKKQEKIIHPFLEEEVEIGLLPYIQAMLLARHLRGDLAEYPPF
                     LMR"
     gene            1095..1388
                     /gene="cas2"
                     /locus_tag="PWY43_002430"
     CDS             1095..1388
                     /gene="cas2"
                     /locus_tag="PWY43_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDL2137254.1"
                     /translation="MLMLITYDISFDDPEGQARLRRIAKHCLDYGVRAQYSVFECDVT
                     PAQWVTLKNKLLATYNPDCDSLRFYHLGSKWRNKVEHHGAKPSVDIFKDTLIL"
     repeat_region   1578..2204
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1578..1609
                     /rpt_unit_seq="gcagccacctacgcgtggctgtgaattgaaac"
BASE COUNT          624 a          455 c          517 g          638 t
ORIGIN      
        1 aggaaattcc aaccatgcgc aaactccaaa acaccctcta tatcaccagc caaggtagtt
       61 acttgcacaa ggaacgtgaa acactagttg tggagcaaga tcgaaaaaaa gtggcacaat
      121 taccgataca ttccatcggg catattttct gttttgggaa tgtattggta tcgccatttt
      181 taatgggatt ttgtggcgaa aataacgtaa atttggcgtt ttttaccgaa aatgggcgtt
      241 atcttgggcg gttgcagggg cggcaaagtg gtaatgtatt gttacgccga gcccagtata
      301 aaaagtcgga gaccaaccct gttcctgtgg ctcgcaacat cattgcagcc aaaatccaag
      361 catctaagcg agtgttacaa cggcaaattc gtaatcacgg cgaaaatgcg gatattcaat
      421 tagcaattac cgcattaaat tacagcttgc aacagttgaa aatagcggac aatttgaatt
      481 tgattcgtgg tattgaaggc gatgctgcgg ctcgttattt tgggatgttt tctcatctta
      541 tcaaagaaaa tagcggtttc cactttgacg gacgtaatcg ccgcccacct cgtgacggtg
      601 tgaatgcttt gctctcattt ctttatagta ttttagggaa agatattagc ggggcattgc
      661 aaggcgtggg gcttgacccg caaattggct ttttgcatgc agatcgtccg gggcgagata
      721 gcttagctca agatattttg gaggaatttc gagcgtggtg ggtggacaga atggtgctgt
      781 cgctgatcaa tcgagggcag gttaagcctg atgattttgt gacggaagcc agtggtgcgg
      841 taacgctcaa gcctgaagca agaaaattac tatttcaaac cttgcaagcg aaaaaacaag
      901 aaaaaatcat tcacccattt ttagaggaag aagtggaaat cggcttgtta ccttatatcc
      961 aagcgatgtt attggctcgt catttgcgtg gcgatttggc agaatatccg ccgtttttaa
     1021 tgcgttaaaa tctgcaaaat tttgttgaaa tttgaccgct tgtttatgtt ttattcattg
     1081 aggaattagc aactatgctg atgttgatta cctacgacat ttcatttgat gaccccgaag
     1141 ggcaggctcg cctacgccgt attgctaagc attgtttgga ttatggtgtg cgggcgcaat
     1201 attctgtgtt tgagtgtgat gttactcctg ctcagtgggt aacattgaaa aataaattat
     1261 tggcaactta caacccagac tgtgatagtt tgcgctttta ccatttaggc agtaaatgga
     1321 ggaataaagt tgagcaccat ggcgcaaaac cttcagtgga tatttttaaa gatacgctga
     1381 ttttataaat cgctagcctg tagttctcat aaaaatgtag caaggttagc gaatctatat
     1441 tgttctttaa taatcaagga attagaaaaa ataaggcaca gtatattggc gatatgctat
     1501 actttatcca ctctctacaa aacgttagcg aataaagggc taaaaagcct gatatttcaa
     1561 tacgttatct tgtaagggca gccacctacg cgtggctgtg aattgaaact tacatattgc
     1621 caaaggcaac ctaaaagacc actcgcagcc acctacgcgt ggctgtgaat tgaaactttt
     1681 tattgttgat tttgacaata gactaatgtt cagccaccta cgcgtggctg tgaattgaaa
     1741 ctattttttg tattttttaa gaattttatt aaactgcagc cacctacgcg tggctgtgaa
     1801 ttgaaactga tttactagac cttttaacaa attataaaaa ttgcagccac ctacgcgtgg
     1861 ctgtgaattg aaacatcatt ccagttttca gccgttcgcc ttgcggtggc agccacctac
     1921 gcgtggctgt gaattgaaac accacctcct ccgagatggt aggcaagggg attagcagcc
     1981 acctacgcgt ggctgtgaat tgaaacaccc gcttgcggta aagctctagg ctctcaccct
     2041 gcagccacct acgcgtggct gtgaattgaa actcttaaat cgctatcgct ctcttcatct
     2101 gcgccaagca gccacctacg cgtggctgtg aattgaaacc aatcaaggcg gccatttcgg
     2161 gggtaaattc gtgcagccac ctacgcgtgg ctgtgaattg aaacagggat aagctcttcg
     2221 ccctcgctgc attg
//
LOCUS       DANKBV010000213         2225 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.214, whole genome shotgun sequence.
ACCESSION   DANKBV010000213 DANKBV010000000
VERSION     DANKBV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.214"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            225..>2225
                     /locus_tag="PWY43_002431"
     CDS             225..>2225
                     /locus_tag="PWY43_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL2137255.1"
                     /translation="MTEEKKARVDADKANAERLTDITSRVANAESNITNFQSTKANKS
                     EVASIAQQNLQSIWRTDAQSAVDALKIGGANLLVDSEYLTTARWGGSSRVASSQYGDR
                     RLTQVFVTQAGTGHFGVTQGTQKATTRIRQGETYTLSLNAQGTAGFTRTGLNYVYLIR
                     EDGGNFRLPTLPLTASLSQRPKVTFTAPWTSNQVRLLIGANGIFEATDWFAFHSVKLE
                     MGNVATGWTPTAKDIDDKVSAVQSNLTAYQAAQAKADQAKATQISGLTTRMGAAESNL
                     TRTERAVTELNQTTVTTLRDLTARTKTTEGSLSRLETAKANKTEVASIAQSSLQSIWK
                     ADAKSAVDSLSIGARNLLIDSTYNQHINYNTKMSSITRTVYRGNMLIRLGMLGGAGVA
                     GIVQAPAAQVSNIRQGQEYTLSLNVQGTAGIQKTGLNYVFLMRADGANQRLATIPVIA
                     SLLNRPKITFTAEWTSERAYLLVGVNGTYETTDWLAFHSVKLEKGNVATDWTPAPEDV
                     DSAVSAVSAKIDTIQETLANADSALSSRIDTVTASVGSNLAKITQVSNAVAGVDGKLS
                     STHTIKTETIAGGRKAIAGIALGAAADNRNAESSVIVMADKFEVVKNAQDGQPVRLFG
                     VAQNKVAINGDLIATGTIKGSHIDADSVRAGILTAGAIRTEHL"
BASE COUNT          675 a          496 c          543 g          511 t
ORIGIN      
        1 ctccaaatca atttgttgag tttcatagtt taaagctaga aaaaggaaac gtagcgacag
       61 attggacacc agcaccggaa gatgtggaca gtgcggttag tgctgtttcg gctgatttaa
      121 cgagttataa acaaactcaa gcggctaccg attcagcaca agcacagcaa cttaatcagt
      181 tatctgtgaa cttgaccaaa gcagagacta atttcaacgc caaaatcacg gaagaaaaaa
      241 aagctcgtgt tgatgcagat aaagcgaatg cagagaggct cacagacata acaagccgtg
      301 ttgcaaatgc ggaatcaaat attaccaatt tccaatcaac caaagcgaat aaaagtgagg
      361 tggcaagtat tgcccagcag aacttacaga gcatttggcg caccgatgcg caaagtgcgg
      421 tggatgcctt gaaaatcggt ggtgcaaatc tactggttga tagtgagtat ttgacgacag
      481 cccgttgggg cggtagctca cgagtggcaa gctcgcaata tggtgatcgt cgcttgactc
      541 aagtgtttgt gacccaagca ggcacagggc attttggtgt gactcaaggc acacaaaaag
      601 ccactacgcg tattcgtcag ggtgaaacct ataccttgtc actaaatgcg caggggacgg
      661 caggttttac ccgcacaggg ttaaactatg tgtaccttat ccgcgaagat ggtggcaatt
      721 ttagattgcc aactttgccg cttactgcat cattgtccca acgccctaaa gtgacattta
      781 ctgcgccgtg gacgagcaat caagttcggt tactgattgg cgcaaatggc atatttgagg
      841 cgacagactg gttcgcattt cacagcgtta agctcgaaat gggtaatgtt gccacaggct
      901 ggacaccaac cgccaaggat attgacgaca aagtatcagc ggttcagtcc aacctcaccg
      961 cttatcaggc ggcacaagct aaagcagatc aagccaaagc tacgcagatt tcggggctca
     1021 caacccgaat gggggcagcg gaaagtaacc tcactcgcac cgaacgggca gtgacggaac
     1081 ttaatcagac aacggtgacg acactgcgtg atctaaccgc gcgcaccaaa acaacggaag
     1141 gttcgctctc tcgtttggaa accgccaaag caaataaaac tgaggtggca agtattgctc
     1201 aaagctcctt acagtcaatt tggaaagctg atgcaaaatc tgccgttgat tcactttcta
     1261 tcggtgcaag gaacttactg atagacagta cttacaacca acacatcaac tacaatacaa
     1321 aaatgtcatc aatcacacgc acagtctatc gcggcaatat gttgatacga ctcggaatgc
     1381 ttggtggcgc aggtgtggcc ggtattgtac aagcaccagc cgctcaagta agtaatatca
     1441 gacagggtca agagtacaca cttagcctga atgtacaggg tacagccggc atacaaaaaa
     1501 cggggctcaa ctatgtattc ttgatgcgtg ctgatggggc taatcaacgg ttagcaacaa
     1561 tacctgttat agccagccta ttaaacagac ctaaaataac ctttactgcc gaatggacaa
     1621 gtgagcgagc ctatctgctt gttggtgtaa atggtactta tgagaccaca gattggttag
     1681 cgtttcacag cgtcaaactt gaaaaaggca acgtagccac agactggacg ccagcacctg
     1741 aagatgtgga cagtgcggtt agtgcagttt cggctaaaat tgatactatt caggagactt
     1801 tagcgaatgc cgactctgca ttaagctctc gtattgatac cgttactgct tctgttggct
     1861 caaacttggc aaaaattact caagtgagta atgctgtagc cggtgtagat ggtaaattat
     1921 cgtctactca taccatcaaa accgaaacca ttgccggtgg acgtaaagcg attgccggta
     1981 tcgcacttgg tgccgcggca gacaatcgca atgcagaatc ctctgtcatt gtaatggcag
     2041 acaaatttga ggtagtaaaa aatgctcaag acggccaacc ggtacgattg tttggtgtgg
     2101 ctcaaaataa agttgcgatt aatggggatt taattgcaac aggtacaatt aaaggtagcc
     2161 acattgatgc agactcagta agagccggaa tattgacggc gggagctatc cgtacggaac
     2221 acttg
//
LOCUS       DANKBV010000214         2119 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.215, whole genome shotgun sequence.
ACCESSION   DANKBV010000214 DANKBV010000000
VERSION     DANKBV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.215"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..267)
                     /locus_tag="PWY43_002432"
     CDS             complement(<1..267)
                     /locus_tag="PWY43_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2137256.1"
                     /translation="MAGNSIGQLFKVTTFGESHGIALGCIVDGVPPNMALSEADLQPD
                     LDRRKPGTSRYTTPRREDDEVQILSGVFEGKTTGTSIGLIIKNGD"
     gene            complement(270..>2119)
                     /locus_tag="PWY43_002433"
     CDS             complement(270..>2119)
                     /locus_tag="PWY43_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL2137257.1"
                     /translation="QINNRLKIVATDSQWNTPLAIFWTLVLCLPSTLMFLAGFIAITY
                     ISFENPQEVWTWGLKMAIYWLYFAFLVAMLRPHGIGHIHFNMPQKSNEIFRNILKRSV
                     WIIALLLNTSIFTNLEMGISYDMLGQTMTIFVLILMIWMIVPAFRQAISTYENVTTEE
                     KKESKSILLTIVKLVLLLAPIILIILIALGYYYTALVIIEHLVDSYFAIITWIIIRNI
                     LYRAFSISSRRLAYRRLQEKREAAEAKARAKAEQNDENPDGVELPFELKEDSIAVSEV
                     KNQMLKLTDFVLWIGLFVLLYWVWSDLITVALYLDGVTLWQQSVTTEAGIVMESITLL
                     NLLTAILIIVVMVVLIRNLSGLLEALIFSHFKLSQGTPYTITTLLTYLIIALGAIFAF
                     GTLGMSWSKLQWLLTALSVGLGFGMQEIFANFVSGIIILFERPMRIGDTITIGTYSGT
                     VSKIRIRATTLVDFDGKEVIVPNKAFVTERLVNWALSSTATRIIVQVGVAYGSDLELV
                     KRLLLQVAEENPKVLKDPQPRAYFLTFGASTLDHELRAYVNDLSDRNPTIDALNTRIH
                     QLFNEHNIDIAFNQLDVYIKNNDTQDEIKVDSKRFEFERPNNITAKEKE"
BASE COUNT          636 a          421 c          429 g          633 t
ORIGIN      
        1 gtcaccgttt ttaatgatta aaccaatgct tgttcccgtc gttttgcctt caaagacgcc
       61 cgataaaatt tgcacttcat catcttcacg gcgaggcgtg gtgtagcgtg aagtaccggg
      121 tttacggcgg tctaaatccg gctgtaaatc tgcttcgctt aaagccatat tcggcggtac
      181 gccatctacg atacagccta atgcaatacc gtgagattcg ccgaaagtgg tcactttaaa
      241 taattgtccg atgctgttgc ctgccattgt tattcctttt cttttgctgt tatgttattt
      301 ggtcgttcaa actcaaagcg ttttgaatct actttaattt catcttgtgt atcgttgttt
      361 ttgatataaa catctaattg gttaaatgca atatcaatat tatgttcatt aaagagctgg
      421 tggatgcgtg tatttagtgc atcaatagtt gggttacgat cagataaatc gttcacatag
      481 gctcttaatt cgtgatctaa agtgcttgca ccaaaggtaa ggaaataagc ccttggttgc
      541 ggatctttca agactttcgg attttcttcc gccacttgta ataacagccg tttgactaat
      601 tctaaatcag acccgtaagc cacgccgact tgaacaataa tgcgggttgc ggtgctgctt
      661 aatgcccagt tcaccaagcg ttcggttacg aatgctttgt ttggtacaat cacttctttg
      721 ccgtcaaaat cgactaaggt tgtggcacga atacgaattt tggaaaccgt accgctgtaa
      781 gtgccgatgg taatggtgtc gccaatccgc attggacgtt caaacaagat gataatccct
      841 gaaacgaagt ttgcaaagat ctcttgcata ccaaaaccca gaccgacaga aagtgcagtt
      901 agtagccatt ggagcttgct ccaagacatc cctaatgtac cgaacgcaaa gattgcccct
      961 aacgcaataa tcaaataggt taagagtgtg gtaattgtgt atggtgtacc ttgcgataac
     1021 ttgaagtgcg agaaaatcag ggcttcaagt aaaccggata agttccgaat gagtaccacc
     1081 attaccacaa taatcagaat agcagtgagt aaattcagca gggtaatgga ttccattaca
     1141 atcccggctt ctgttgtaac actttgttgc cataaggtaa cgccgtctag gtagagtgca
     1201 acagtaatta agtccgacca aacccagtaa agcagcacaa ataagccaat ccaaagcaca
     1261 aaatcagtta atttcaacat ttggttcttg acttcgctta cggcaataga gtcttctttt
     1321 agctcaaaag gaagctcgac accgtccggg ttttcatcat tttgttctgc tttagctcgt
     1381 gctttggctt cagctgcttc tcttttttct tgtaaacgtc tgtaagccag acggcgtgat
     1441 gaaatagaga atgctcgata gagaatatta cgaataataa tccaagtaat gatcgcaaaa
     1501 taagaatcta ctaaatgctc aatgatcacc agtgcagtat agtaatagcc caatgcaatc
     1561 agaatgataa gaatgatagg ggcaagcagt agcactaatt tgacaatagt gagtaaaata
     1621 cttttagatt cttttttctc ttctgttgtg acattctcat aggtggaaat agcttgccta
     1681 aatgccggta caatcatcca aatcattaat ataaggacaa aaatagtcat tgtttgtcca
     1741 agcatgtcgt aagatattcc catctctaag tttgtaaaaa tagaggtgtt cagtaataaa
     1801 gcaatgatcc ataccgaacg ttttaaaata ttacggaaga tttcattact tttttgtggc
     1861 atattaaagt gaatatgtcc tatgccgtgc gggcgtagca tcgccactaa aaaggcaaaa
     1921 tacagccaat aaattgccat ttttaatccc caagtccaga cttcctgagg attttcaaat
     1981 gaaatataag tgatagcaat aaatccggct aagaacatta gtgtacttgg caggcaaagc
     2041 actaatgtcc aaaaaatagc aagaggggtg ttccattggc tatctgtggc gacaattttt
     2101 agtcgattat taatttggt
//
LOCUS       DANKBV010000215         2110 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.216, whole genome shotgun sequence.
ACCESSION   DANKBV010000215 DANKBV010000000
VERSION     DANKBV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.216"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            169..885
                     /locus_tag="PWY43_002434"
     CDS             169..885
                     /locus_tag="PWY43_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDL2137258.1"
                     /translation="MYLMYVDESGSTTDPTQKYFVLSGIAVFERNTHWIEKKMNVIAS
                     QFCQYSEGSDPYFYELHGSPMRSGKSEWRGIPMSERINAIQDCLKLVQSNNIRIFAAV
                     IEQGYSSGQDTITECFEQISSRFDMYLSRLHAQGNTQRGISIFDKSSTEKSIQALART
                     FKNSGHTYGKLRNFSEVPLFLDSKSSRLIQLADLVSYAIFRHYEHNDSSFYDIIRNNF
                     DYHNGRIHGLYLRNKEDIIS"
     gene            complement(928..1092)
                     /locus_tag="PWY43_002435"
                     /pseudo
     CDS             complement(928..1092)
                     /locus_tag="PWY43_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1215..2003)
                     /locus_tag="PWY43_002436"
     CDS             complement(1215..2003)
                     /locus_tag="PWY43_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137259.1"
                     /translation="MFLSHFKQVLNANIEADGKDWTIIDVFGGSGLLAHTAKREKPLA
                     RVIYNDFDNYAERLNHIKDTNQLRQEIYQIVDGVIPKNKRISNEVKAKIINKINDFEG
                     FKDPKCLSSWLLFSGEQVATLDELFKHDFWNCVRQSNYPEATGYLDDIEVVSESFHQL
                     LPRFHDKEKVLLILDPPYLCTRQESYKQANYFDLIDFLRLIDLTKPPYIFFSSTKSEF
                     IRFIHYMVQNKKENWQAFEGAERIVVNASPSYNGKYEDNMVFKF"
BASE COUNT          664 a          380 c          362 g          704 t
ORIGIN      
        1 cttgattttt gtcaaaaagt ctctatacta tatggcaggg tcagttttcg ctgtttaacg
       61 gtgtttaatg agattttcat tactggctct ccttaataga aatttatgga agagctatct
      121 gatagagtaa tctagcgata gctctttttc attgaacaaa gaggctatat gtatcttatg
      181 tatgttgatg agtctggttc tacgacagat cctactcaaa aatattttgt attatcaggt
      241 atcgctgtat ttgaacgaaa tactcattgg attgagaaaa aaatgaatgt gatagcctct
      301 caattctgtc aatattctga agggagtgat ccatatttct atgagttaca tggttcacca
      361 atgagatctg gaaagagtga atggagaggc attccaatga gtgagcgtat taatgccatt
      421 caagattgct tgaaattagt tcaaagtaat aatattcgta tatttgcagc agttatagag
      481 caagggtatt ctagtggtca ggatactatt acagaatgtt ttgagcaaat atcatctcga
      541 tttgatatgt atctgagccg tttacatgcc caaggtaata cccaaagagg catttctatc
      601 tttgataaat catcgactga gaagtcaatc caagcacttg caagaacatt caaaaatagt
      661 gggcatactt atgggaaact tagaaatttc tcagaagttc cattatttct tgactcaaag
      721 tcatcgagat taattcagct agcggatcta gtttcctatg cgatatttag acactatgaa
      781 cacaatgata gctcatttta cgatattata agaaataact ttgattatca taacgggcgt
      841 attcatggtt tatatttacg gaacaaagaa gatattattt cataaaaatt cgccccaaaa
      901 gggagaagta tctccctttt gtatttttta taacaaatta accgtttctc tcaacatttc
      961 cacatcgtgt tcaatatatc ctagtgtcac actgatatct ttatgcccta acagtttctg
     1021 aaccacataa acattttcgg gcttcttcat taattgagta gcaactgtat gtcgaaatct
     1081 gtgtggtgat gtaagcggtc gaattatttt gcatagaatt acactcaatt ttgcatagat
     1141 aaaaattaaa aactatgcaa aataaatcgc aaaaacaaac gccctttaaa ccataattaa
     1201 gggcgtttaa atttttagaa cttaaaaacc atattatcct catatttgcc gttataactt
     1261 ggcgaggcat ttactactat tctttcagcc ccctcaaacg cttgccagtt ttcttttttg
     1321 ttttgaacca tatagtgaat aaagcgaata aactcgctct tggttgagct aaagaagata
     1381 taaggcggtt tggtaaggtc tatcaatcgt aggaaatcaa ttaaatcaaa gtaatttgcc
     1441 tgcttataac tctcctgtct tgtgcaaaga taaggcgggt caagaatcaa taatactttc
     1501 tctttgtcgt gaaacctcgg caataattga tgaaaggact cactaaccac ctcaatatca
     1561 tcaagataac ccgttgcttc aggatagtta ctttgccgca cacaattcca gaaatcgtgc
     1621 ttaaataact catctaaagt agcaacttgc tcaccactaa ataacaacca agaacttaag
     1681 catttggggt ctttaaaccc ctcaaaatca ttgattttat tgatgatttt agctttaact
     1741 tcattgctaa ttctcttatt ttttggtata actccatcaa caatttggta gatttcttgg
     1801 cggagttggt tagtgtcttt gatatggttt agccgttcgg cgtaattatc aaaatcatta
     1861 taaatcactc ttgctaacgg tttttcacgc tttgcagtat gtgcaagcaa cccactccca
     1921 ccaaaaacat ctataatagt ccaatctttc ccgtctgctt caatatttgc atttaacacc
     1981 tgtttgaaat gggataaaaa catccgcttt tgaccgataa aaggtagcgg agcttgttta
     2041 aatattacat ttgcttgctg tttagtttgt acttgcgatg ccatcgcagc aacagctaat
     2101 gcggttaaag
//
LOCUS       DANKBV010000216         2042 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.217, whole genome shotgun sequence.
ACCESSION   DANKBV010000216 DANKBV010000000
VERSION     DANKBV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.217"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(269..1111)
                     /locus_tag="PWY43_002437"
     CDS             complement(269..1111)
                     /locus_tag="PWY43_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137260.1"
                     /translation="MAKQIFKQAPLPFVGQKRMFLAQVSQILNENIPDDGQGWTIIDV
                     FGGSGLLAHTVKHIKPKAHIIYNDYDGYAERLKHIPDTNRLRKQIYDIIGESTPKNKR
                     LDPDKKSQVINVIQSFDGYIDVNCVASWLLFSGQQINSLENLFNKIFWNGIRQTDYPS
                     AEGYLDGIEVTHESFHKLLPRFQHKDKVLLLLDPPYLCTRQESYKQATYFDLIDFLRL
                     INLTKVPYIFFSSTKSEFVRFVDFMVSEKKDNWQTFENAKWIKVKASLNYQSTYEDNL
                     VYKF"
     gene            complement(1158..1283)
                     /locus_tag="PWY43_002438"
     CDS             complement(1158..1283)
                     /locus_tag="PWY43_002438"
                     /inference="COORDINATES: protein motif:HMM:NF021626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2137261.1"
                     /translation="MQSLRLIEIRCKYCQKLLAKAKDVQHLEIKCVRCKSINKFN"
     gene            complement(1430..1639)
                     /locus_tag="PWY43_002439"
     CDS             complement(1430..1639)
                     /locus_tag="PWY43_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137262.1"
                     /translation="MFRNELQVMDGKRYVVLECQFRREWKVAIESRGTVTSGEAIEIC
                     QYWIKYKGVKPEQLKVVEVPDILKE"
     gene            complement(1660..>2042)
                     /locus_tag="PWY43_002440"
     CDS             complement(1660..>2042)
                     /locus_tag="PWY43_002440"
                     /inference="COORDINATES: protein motif:HMM:NF016343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL2137263.1"
                     /translation="EAYEFASEMDDCENYEANDLPYAVYGVVLGRAKSDIRPLTSEEK
                     ESGYFEEVDNFIEQPELVETNGWISVKDKLPEIHEEVLVTNNFIMIAKFNGESWITSG
                     GILGIQPVYDVTHWQPLPEPPREQ"
BASE COUNT          605 a          430 c          316 g          691 t
ORIGIN      
        1 ccaagttcag ccattttttc aatcctattt cgcaaaaaag ggactgtctc caacaacaga
       61 aagcaacgcc tgaaatccga tacatcgtgt gggtagcctt ctatttttgg tcttattccg
      121 taaccaaggt aaaaagccat tgttttactg cttacccctg tctctccatt ggcaagccac
      181 caagcgattt tgtcttctat gtgcattttt cctccaaaaa ataaccgctt gcggaacaag
      241 cggttaattg tcaatcattc attaaatgtc aaaatttata aaccaaatta tcttcatagg
      301 tagattgata atttagacta gctttaactt taatccattt cgcattttca aaagtttgcc
      361 agttatcttt tttctcgcta accataaaat ctacgaacct cacaaattca ctttttgttg
      421 aactaaaaaa gatataaggc actttagtta aattgattaa tcttaaaaaa tcgattaaat
      481 caaaataagt ggcttgctta tagctttctt ggcgagtaca aagatatggc ggatcaagta
      541 ataacaatac cttatcttta tgctgaaaac gtggtaatag tttatgaaaa ctttcgtgag
      601 taacttctat tccatctaaa tagccttccg cacttggata atcggtttgg cgaataccat
      661 tccaaaatat tttgttaaat agattctcta aactattaat ttgctgtccg ctaaatagca
      721 accaagaagc cacacaattt acatcaatat acccatcaaa agactgaata acattgataa
      781 cttgagattt cttatcagga tctaatcgct tgtttttagg cgtactctcc ccaataatgt
      841 cataaatttg cttgcgtagc ctatttgtat cgggaatatg ctttagccgc tccgcatatc
      901 catcataatc attgtagatg atgtgagctt ttggtttgat atgtttgaca gtatgagcta
      961 ataacccact accaccaaat acatctatga ttgtccaacc ttggccgtca tcaggaatat
     1021 tctcatttaa aatttgagaa acctgagcta gaaacattcg tttctgacct acaaacggaa
     1081 gtggggcttg tttaaagatt tgctttgcca tagttctcct atggcgttca ggcgttctta
     1141 acgctcagac actcaattca gttaaattta ttgattgatt tacaacgtac acatttaatt
     1201 tctaaatgtt gtacatcttt cgcttttgcc aataattttt ggcaatactt acagcggatc
     1261 tcaattaatc ttaatgattg catataaata cacttttact tcattttttg ataaaattcc
     1321 gcctacctag ctaggtggcg gcgtatggct atatgcaagt gtgctttgcg tagctggcat
     1381 tataagtgtt gctgcactta taatgtcgcc gttcttaact aacttatgcc tattccttca
     1441 aaatatcagg cacttcaaca actttaagct gctcaggttt tacaccttta tacttgatcc
     1501 aatactgaca aatttcgata gcttcgccac tcgttacagt acctctagac tctattgcaa
     1561 ctttccattc gcggcgaaat tggcattcaa gcacgacata acgtttaccg tccattactt
     1621 ggagttcatt tctaaacata tctaaaacct tacaggattt tattgttccc ttggtggttc
     1681 aggaagtggt tgccaatgag taacatcgta aacaggctga atacctaaga tacctcccga
     1741 tgtaatccag ctttcgccat taaacttagc tatcataata aagttgtttg ttaccaacac
     1801 ttcttcgtgg atttctggca atttgtcttt aaccgaaatc cagccattag tttctacaag
     1861 ttctggttgt tcaataaaat tgtcaacctc ttcaaagtac ccagattcct tttcttcact
     1921 tgtaagcggg cgaatatctg attttgctct acctaaaaca acgccataaa ctgcatatgg
     1981 taaatcattg gcttcatagt tttcgcaatc atccatttca ctggcaaact cataggcttc
     2041 ag
//
LOCUS       DANKBV010000217         2003 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.218, whole genome shotgun sequence.
ACCESSION   DANKBV010000217 DANKBV010000000
VERSION     DANKBV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.218"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            32..1114
                     /gene="aroG"
                     /locus_tag="PWY43_002441"
     CDS             32..1114
                     /gene="aroG"
                     /locus_tag="PWY43_002441"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDL2137264.1"
                     /translation="MSYIKNDDVRITSIEELLPPIALLEAYPASDVAAETVAKTRQSI
                     HKILHGADDRLLVVIGPCSIHDPKAALEYAQKIKAMRADPKINQNLEVVMRVYFEKPR
                     TTVGWKGLINDPYLNETFALNDGLRIARKVLSDINDLSVPTAGEFLDMITPQYVADFM
                     SWGAIGARTTESQVHRELASGLSCAVGFKNGTNGSVKIALDAISSAKSPHHFLSVTKF
                     GHSAIVSTAGNPDCHIILRGGDSATNYDADSVAAACAAIEKSGGRPHVMIDFSHANSQ
                     KQFKRQMDVCADVCNQIAGGSDFISGVMIESHLVEGRQDLIEGKGLEALVYGQSITDG
                     CIGWEDSEKALFALAQAVEKRRQFAK"
     gene            1197..1889
                     /gene="tsaB"
                     /locus_tag="PWY43_002442"
     CDS             1197..1889
                     /gene="tsaB"
                     /locus_tag="PWY43_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL2137265.1"
                     /translation="MKSTILALDTATEACSVALQHNGNITFLEEISPRSHTQRILPMV
                     DELLHQANIELKEVNYLVFGRGPGSFTGVRVGVSVAQGLAMGANLSVVAVSNLKAMAE
                     EAYQKLGATKVIALIDARMNEVYFAQFERKGAEWNEVVAEQVCSPEAAIGQFQVSEET
                     VVVGTGWAAYLQFSEQNLPLVISDITLPSSRYMLSIAETEILKGNTLSALEIEPVYLR
                     NEVTWEKLPHKQ"
BASE COUNT          599 a          389 c          456 g          559 t
ORIGIN      
        1 ttattttcat tttatttaga gagtgaacac aatgtcttat atcaaaaatg acgatgtcag
       61 aattaccagt attgaagaat tactaccacc tattgcatta ctggaagcct atccggcaag
      121 tgatgttgca gcggaaaccg ttgctaaaac ccgtcaatca atccacaaaa ttttacacgg
      181 tgcggatgat cgcttattag tggtgattgg accttgctcc attcacgacc cgaaagcggc
      241 attagaatat gcacaaaaaa ttaaagcaat gcgtgctgat ccgaaaatta accaaaactt
      301 agaagtggtg atgcgtgtct attttgaaaa accccgtaca accgttggtt ggaaaggctt
      361 aattaacgac ccttatttga acgaaacctt tgccttaaac gatggtttac gcattgcccg
      421 taaagtatta tctgatatta atgatttaag cgtgccgact gccggcgagt ttttggatat
      481 gattacacca caatatgtgg ctgactttat gagctgggga gcaattgggg cgagaacgac
      541 tgaatctcaa gtccaccgtg aattagcctc tggtttatcc tgtgcggtag gttttaaaaa
      601 cggcacgaat ggtagtgtga aaattgcatt agatgcaatt tcttccgcaa aatcgccaca
      661 ccatttctta tcggtcacta aattcgggca ttctgcgatt gtttcaacag caggaaatcc
      721 tgactgtcac attattttgc gtggtggcga tagcgcaacc aactacgatg ctgactccgt
      781 tgcagcggct tgtgctgcga ttgagaaatc cggcggacgt ccacacgtga tgatcgattt
      841 cagccacgct aacagccaga agcagttcaa acgccaaatg gacgtttgtg ctgatgtgtg
      901 caaccaaatt gcaggtggat cagactttat ttcaggtgtg atgattgaaa gccacttggt
      961 agaaggtcgc caagacttaa ttgaaggcaa aggtttagag gcgttagttt acggacaaag
     1021 tattacagac ggttgtattg gttgggaaga tagcgaaaaa gcgttatttg cactggcaca
     1081 agcggtcgaa aaacgccgac aatttgcaaa ataatctttt cattgagggc gaatacaatt
     1141 cgccctttct ttgtttaacc ttaaagcaaa ttcaatttgt tcaaactaaa aatatgatga
     1201 aatctactat tcttgcttta gatactgcca cagaggcttg ctctgtagcg ttacaacata
     1261 acggaaatat cactttctta gaagaaatca gccctcgttc ccatacccag cgaattttgc
     1321 cgatggtcga tgaattactt catcaagcca atatcgaact caaagaagta aattatttag
     1381 tattcggacg tggtccgggc agttttaccg gcgtgagagt aggtgtaagt gtggctcaag
     1441 ggttagcgat gggggcgaat ttgtcggtgg tcgctgtttc caacttaaaa gcaatggcag
     1501 aagaggcata tcaaaaatta ggggcaacaa aggtaattgc cttaattgat gcccgaatga
     1561 atgaagttta ctttgctcaa tttgaacgca agggtgcaga atggaatgaa gtagtggctg
     1621 aacaagtctg ttcgcccgaa gctgcaatag gccaatttca agtaagtgaa gagactgtgg
     1681 tggttggcac aggttgggcg gcatatttgc aattttctga gcaaaatcta ccgcttgtta
     1741 ttagcgatat cactttgcct tccagtcgat atatgctttc gattgctgaa actgaaatcc
     1801 taaaagggaa tactctatct gcccttgaaa tagaaccggt gtatttgcgt aatgaagtca
     1861 cttgggaaaa attaccgcat aagcaataac actacgcttt ttgctaaata aagaagtatt
     1921 gcaagaaccc cgtataacaa gcaatgccaa ataagatcat acctagtagc ttcgtggtta
     1981 attcggggct taaaatattg cgc
//
LOCUS       DANKBV010000218         1953 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.219, whole genome shotgun sequence.
ACCESSION   DANKBV010000218 DANKBV010000000
VERSION     DANKBV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.219"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(72..1763)
                     /gene="dld"
                     /locus_tag="PWY43_002443"
     CDS             complement(72..1763)
                     /gene="dld"
                     /locus_tag="PWY43_002443"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDL2137266.1"
                     /translation="MSNNSLISQLTEIVGQSYIITDPTKTEAYRSGYRFGTGSAIAVV
                     KPTTLLEYWNVLKACVAADVIVISQAANTGLTGGSTPSGNDYDRDIVIINTMRMNGIQ
                     LINNANQVVCLPGSTLNELELLLKTHGREPHSVIGSSCIGASVIGGICNNSGGALVQR
                     GPAYTEMALFAQLNEDGTLELKNHLGIDLGDTPEEILENLQAQRYQRKDIQQACGKGH
                     DHAYCNHVRQIDEDSPARFNADPARHYEAAGSAGKLAVFAVRLDTFPLEKDTAVFYIG
                     TNHTDTLNDIRRYMLANFEVLPISGEYIHRDAFDIAADYGKDTFWVIKKLGTHRLPQL
                     FSLKAKVDRMAKKLTFLPANLSDKILQAISKMLPQHLPESLRDYRNQYEHHLIIKMGG
                     KGVQEARDYLARYFADTQKGGYLECNPTESQAAMLHRFAVASAAIRYRAIHDKEVEDI
                     VALDIALRRNDKAWFEVLPPEIESQITHKLYYGHFMCHVFHQDYIVKKGVDCLALEHQ
                     MLELLDKRGAQYPAEHNVGHLYEAKPTLRKFYRQLDPTNSFNPGIGQTSKKKYWK"
BASE COUNT          544 a          439 c          402 g          568 t
ORIGIN      
        1 tagcaactgt tttgtccact tggcatagtt tttgacggat cagcaactat tttgtctact
       61 atgccttttt gttacttcca gtattttttc ttcgaggttt gcccgatccc cggattaaag
      121 ctgttggttg gatcaagctg ccggtagaat ttgcgtaagg tcggttttgc ttcgtaaaga
      181 tgaccgacat tatgctccgc agggtattgt gccccacgtt tatccagtaa ttctaacatc
      241 tgatgttcca acgccaaaca atctacacct tttttcacaa tatagtcttg atggaaaaca
      301 tggcacataa aatgcccata ataaagtttg tgcgtgattt gactctctat ttccggcggt
      361 agcacttcaa accacgcttt atcatttcgg cgaagggcaa tatctaaagc aacaatatct
      421 tccacctctt tatcgtgaat ggctcgatag cgaatcgctg ccgaagcaac ggcaaaacga
      481 tgtaacattg ccgcttggct ttcagtcgga ttacactcca aataaccgcc tttttgtgta
      541 tcagcaaaat aacgggcaag atagtctctt gcttcttgaa cgccttttcc acccattttg
      601 ataattaagt gatgttcata ctggttacgg tagtctcgaa gactttccgg caaatgttgc
      661 ggtaacattt ttgagattgc ctgtaatatt ttatcactga gatttgccgg taaaaaggtg
      721 agtttcttcg ccattctgtc cacttttgct ttaagtgaaa agagttgtgg taagcggtgt
      781 gtaccgagtt ttttaatcac ccaaaaagta tctttaccgt aatcagcggc aatatcaaag
      841 gcatcacgat gaatatattc accggaaatc ggcaatacct caaagttggc taacatatag
      901 cgacgaatat cgtttaatgt gtccgtatga tttgtcccga tataaaaaac agccgtgtct
      961 ttttcaagcg gaaaggtatc taatcgcacc gcaaacaccg ctaactttcc tgcactaccg
     1021 gcagcctcgt agtggcgggc aggatcggca ttaaaacggg caggggagtc ttcatcaatt
     1081 tggcggacgt gattacaata ggcgtgatcg tgtccttttc cgcacgcttg ctgaatatcc
     1141 ttacgctgat aacgttgggc ttgcagattt tctaaaattt cttccggcgt atcacctaaa
     1201 tcaataccta aatggttttt taattccagc gtgccgtctt catttaactg ggcaaacagt
     1261 gccatttcag tataagccgg cccacgttgc actaatgcac cccctgaatt attacaaata
     1321 ccaccgataa ccgaagcccc gatacaggac gatccgatca ccgaatgcgg ttcacgtccg
     1381 tgcgttttca gcaacaattc cagctcattt aatgtcgagc ccggtaagca aacgacttga
     1441 ttggcattgt taattaactg aatgccgttc attcgcattg tgttaatgat cacaatatcc
     1501 cgatcataat cgttgccgct tggtgttgaa ccaccggtta atccggtatt ggccgcttgt
     1561 gagataacaa tgacatcggc ggcgacacag gcttttaata cgttccagta ttctaataac
     1621 gttgtaggtt ttaccactgc aatcgcacta cccgtgccaa agcgataccc gctacgatag
     1681 gcttccgtct tagtcgggtc agtgatgata taagactgcc cgacaatttc agtaagctga
     1741 gaaatcagtg aattatttga cattatgctt ccctcaaagg tagaaataga tagggcagta
     1801 tagcaataaa caaaagagag caaagcagaa tgttacaaaa ttgtgaacta ctgcacagaa
     1861 taaatgaaga aatgaagtaa aagaaagcga gctaaaagag cgtcgcccgc tttatttgta
     1921 gaaaatcaaa cctaatctga cagtcccccg ttt
//
LOCUS       DANKBV010000219         1893 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.220, whole genome shotgun sequence.
ACCESSION   DANKBV010000219 DANKBV010000000
VERSION     DANKBV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.220"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..382
                     /locus_tag="PWY43_002444"
     CDS             <1..382
                     /locus_tag="PWY43_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2137267.1"
                     /translation="VLLQKREAKGIWGGLFAFPQFESLEALKRSDFMQNLQISQQLTA
                     FRHTFSHFHLDITPVLVETDLKKTSENSPLVVEEKAGNYLSKVSSNADYWYDLHQHNE
                     VGLATPVKRILDELAFSVKNEIKD"
     gene            386..655
                     /locus_tag="PWY43_002445"
     CDS             386..655
                     /locus_tag="PWY43_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDL2137268.1"
                     /translation="MARTVFCEYLKQEAEGLDFQLYPGELGKRIFDSISKQAWSEWIK
                     KQTMIVNEKKLSMMNPEHRKLLEQEMVNFLFEGKEIIIEGYKPVE"
     gene            672..1763
                     /gene="mltC"
                     /locus_tag="PWY43_002446"
     CDS             672..1763
                     /gene="mltC"
                     /locus_tag="PWY43_002446"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDL2137269.1"
                     /translation="MKKYAKYLPMLAIIPFLIACGSNKSTKKPKSTRVDYSKDTNGLD
                     ILAGQFSHNIDDIWGSNELLVASKKDYVKYTDKFYTRSHISFEEGVITIETLGNQNHL
                     RNSIIHTLLMGRDPKGIDLFASGDAPISSNPFLNGQVEDQFGRNITNVAIANDFATYL
                     LQNKVQTRRLKNGQTVSFVTIKMVAGHIEVRARQYLPLVRKMAKQYGIEPSLILGIME
                     VESAFNPYAISYANAIGLMQVVPRTAGRDIFARKGMGGQPSREYLYNPSNNIDAGTLY
                     LTILRDEYLDGIEDPVAKRYAMISAYNSGAGAVLRVFDSDRYSAIDRINDLSPDAVYR
                     ILTTAHPSSQARNYLRKVTTAREKYRNIR"
BASE COUNT          597 a          319 c          421 g          556 t
ORIGIN      
        1 agtgctgtta caaaaacggg aagcaaaagg catttggggg ggcttatttg cgtttccgca
       61 atttgagagc ttagaggctc tcaagcggtc tgattttatg caaaatttgc aaatttcaca
      121 gcaattaact gcatttaggc atacttttag ccattttcat ttggatatta ccccggtttt
      181 agtagaaacc gatttgaaaa aaacaagtga aaattcaccg cttgttgtcg aagaaaaagc
      241 aggaaattat ctttctaagg tatcttcaaa tgcggattat tggtatgatt tgcaccaaca
      301 taacgaagtg ggtttagcaa cgccggttaa acgtatttta gatgagctgg cattcagtgt
      361 gaaaaatgag attaaggatt aaattatggc tcgtaccgta ttttgcgaat atttaaaaca
      421 agaggcagaa ggtttagatt ttcaacttta tccgggggag ctgggtaagc gtatttttga
      481 ctcaatcagc aagcaagcgt ggtctgagtg gattaaaaaa cagacaatga tcgtcaatga
      541 gaaaaaactc agtatgatga accccgaaca ccgtaaactg ctcgaacaag agatggtcaa
      601 ttttttattt gaaggtaaag agatcatcat cgaaggctat aagccggtcg aataataaag
      661 gttaaatcac aatgaaaaaa tatgctaaat atttgccgat gttggcaatt attccgtttc
      721 ttattgcttg tgggagcaat aaatcgacta agaagccaaa atctacccga gtagattatt
      781 ctaaagatac caatggtttg gatattttgg cagggcagtt ttctcacaat attgatgata
      841 tttggggaag taacgagctg ttagttgcca gtaagaaaga ttatgtgaaa tatacggata
      901 agttttacac ccgtagccat atttcgtttg aagaaggggt gattacgatt gagactttag
      961 ggaatcagaa tcatctacgc aattcgatta ttcatacttt attaatgggc agagacccga
     1021 aagggattga tttatttgcc tcaggcgatg caccgattag ctctaaccct ttcctaaacg
     1081 gacaggttga agatcaattc gggcgaaata ttaccaatgt tgccatcgcc aatgattttg
     1141 cgacttattt gttgcaaaat aaggtgcaaa cacgccgttt gaaaaatggt caaactgtga
     1201 gctttgtaac gattaaaatg gtggcaggac acattgaagt acgtgccaga caatatttac
     1261 cgctggtgcg taaaatggcg aagcagtatg gtattgagcc aagtttgatt ttagggatta
     1321 tggaagtcga atcagcattc aacccttatg ccatcagcta tgccaatgcg attggcttaa
     1381 tgcaggttgt acctcgtacc gccgggcgtg atatttttgc ccgtaaaggt atgggagggc
     1441 agccaagtcg tgaatatctt tataatccat caaataacat tgatgcaggt acgctttatt
     1501 tgactatttt gcgtgatgaa tacttagatg gaattgaaga tcctgtggct aaacgctatg
     1561 caatgatttc tgcatacaat agcggagcag gtgcggtatt acgagtgttt gattcagacc
     1621 gatactctgc gattgataga attaacgatc tttcgcctga tgcggtttat cgaattttaa
     1681 ccactgctca tccatcttct caagctagaa attatttaag aaaagtaacc accgccagag
     1741 agaaatatcg caatattcgt tagtgtgtga attatgctgt tttttagccg aaaagtataa
     1801 aaaaagcaca tttgagcagc ggttttttta aatttgcaaa aaaacgtttg acgttgcaag
     1861 ctgaaatcag cataatgcac cacgcaacga cgt
//
LOCUS       DANKBV010000220         1874 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.221, whole genome shotgun sequence.
ACCESSION   DANKBV010000220 DANKBV010000000
VERSION     DANKBV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.221"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..478
                     /locus_tag="PWY43_002447"
     CDS             <1..478
                     /locus_tag="PWY43_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2137270.1"
                     /translation="EVGDWSSGATPNRHNPEYYNGTIPWLKTGDLNNGIISSIPEFIT
                     DKALQECSVRLNPVGSVLIAMYGATIGKLGILKIAATTNQACCACIPFNGIYNKFLFY
                     YLMSQKAEFQKKSEGSGQPNISKEKIINYLFPLPPIHEQHRIVQKIEQLFAEIEKL"
     gene            581..1384
                     /locus_tag="PWY43_002448"
     CDS             581..1384
                     /locus_tag="PWY43_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2137271.1"
                     /translation="MIHLFEKHLNQSNLAENTQSAYLFAVKQYHKKFDQLSKINLQRY
                     KVFLIENYKPQTVNLRLRAINYYLEFIKKEKWKLSFVKVQQKPFLENVISEADYTYFK
                     KCLKKDNELYWYFVIRFMAATGSRVSELIQIKCEHIKIGYLDLYSKGGKLRRIYIPKS
                     LQKECSEWLRLNNKHNGFIFLNKYGDRITTRGIASQLKVFARRYKLDPKIVYPHSFRH
                     RFAKSFLERFNDIAFLADLMGHESIETTRIYLRKTSTEQQEIVNKIIDW"
     gene            complement(1430..>1874)
                     /locus_tag="PWY43_002449"
     CDS             complement(1430..>1874)
                     /locus_tag="PWY43_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2137272.1"
                     /translation="NYSLNHDRRRKPVSVAQRSQQNKLYDYYGATGAIDKVASYIFDG
                     KFILIGEDGGNFFTKKDVAFIVEGKFWANNHVHVLSVDFNLEKYFCYYLNALNLPSMG
                     LINGIAVPKLNQRNLNSILIAIPPISEQHRIVEKIEKLFSEIEKF"
BASE COUNT          677 a          288 c          290 g          619 t
ORIGIN      
        1 ggaagtaggt gattggagtt caggtgcaac tccaaaccga cacaatcctg aatactataa
       61 tggtacgatc ccgtggctaa aaacaggaga cttaaacaac ggaataattt catctatacc
      121 tgaatttatt acagataaag cattgcagga atgttctgta agattaaatc ctgttggttc
      181 agttttgata gcaatgtatg gagcgacaat tggtaagctt gggatattaa aaatagcagc
      241 aacaacaaat caggcttgct gtgcgtgtat tccttttaat ggaatttata ataaattctt
      301 attttattat ttgatgtcgc agaaagcaga atttcagaaa aaaagtgagg gaagtgggca
      361 accaaatata tcaaaggaaa aaattatcaa ttatttattt cctttaccac caatccacga
      421 acaacatcgc attgttcaaa aaattgaaca attatttgca gagattgaaa aattgtagga
      481 gtcatcatcg ctttcttggg cgtgttggat acgccctaca gtcactaaat tattgatctt
      541 ttatcatatc ggttttcaac caacctaaaa ggaaacctat atgatccatc tatttgaaaa
      601 gcaccttaat caatctaatc ttgctgaaaa tacccaatca gcttatcttt ttgcggtaaa
      661 acaatatcac aaaaaatttg atcagttatc taaaatcaat ttacagcgat ataaggtatt
      721 cttaattgaa aactataagc ctcagactgt taatttaagg ctaagagcca taaattatta
      781 tctggaattt attaaaaaag aaaaatggaa attatccttt gttaaagttc aacaaaaacc
      841 atttttggaa aatgtaatta gtgaagctga ttatacttat tttaaaaaat gcctaaaaaa
      901 agataacgaa ctatactggt attttgtgat tcgttttatg gcagcaacag gttcgagagt
      961 aagtgaatta atccagataa aatgtgaaca tattaaaata ggctatttag acctctattc
     1021 taaaggtgga aaattaagac gaatttacat cccaaaatct ctgcaaaaag agtgttctga
     1081 atggttgaga ttaaataata agcataatgg atttattttc ttaaataaat atggtgatag
     1141 aattactact cgtggcattg ccagccaatt aaaagtattt gctagacgct ataaattaga
     1201 tcctaaaatt gtttatccac attcatttcg ccatcgtttt gcaaaaagtt ttttagaacg
     1261 atttaatgat attgcttttc ttgctgattt aatggggcac gaaagcatag aaaccacaag
     1321 gatctattta cgaaaaacaa gcacagaaca gcaggaaatt gtgaataaaa ttattgattg
     1381 gtaattttta aggggcgtgt ttcacacgcc cttggacata taagtttttc taaaatttct
     1441 ctatttccga aaataatttt tcaatttttt caacgattct atgttgttca gaaataggtg
     1501 gaatagcaat taaaatagag tttaggtttc tttgatttaa tttgggtaca gctattccgt
     1561 taattaatcc catactaggt aaatttaatg catttaaata ataacaaaaa tatttttcca
     1621 aattaaaatc aactgataat acatgaacat gattatttgc ccaaaattta ccttcaacaa
     1681 taaaagcaac gtctttttta gtgaagaaat tacctccatc ttctccaatt aaaataaact
     1741 taccatcaaa aatatagctt gccactttat ctattgctcc agtcgctcca taatagtcat
     1801 acaatttatt ttgttggcta cgctgtgcaa ctgatactgg ttttcttctt ctatcatgat
     1861 ttaaagaata attt
//
LOCUS       DANKBV010000221         1843 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.222, whole genome shotgun sequence.
ACCESSION   DANKBV010000221 DANKBV010000000
VERSION     DANKBV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.222"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..174)
                     /locus_tag="PWY43_002450"
     CDS             complement(<1..174)
                     /locus_tag="PWY43_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDL2137273.1"
                     /translation="MQDFIHQANHYGQAKRPFFFLIDFEQKKPLIFPLEQAHASGLFF
                     EFFCKSNLEKPVKK"
     gene            284..>1843
                     /gene="rmuC"
                     /locus_tag="PWY43_002451"
     CDS             284..>1843
                     /gene="rmuC"
                     /locus_tag="PWY43_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDL2137274.1"
                     /translation="MHFQEIGIYIALAVCVLVGVYLFFLKSRYQRDAYELSQDLGKIT
                     ENFEQIQQKYEVLAQEKNQLEQWAIQQQTKYEAVSERLNERDGQLQRFQQKIELAEQQ
                     ENQLERYINELKERVGSSQAKAESLEEQLQFSQSSLSTKERENQALFSRLNEVQNELT
                     ELRTTLSEKQANFEAQQRNFIEVKQQLNVEFQHLAQQILDEKSKRFSETNQSSLEALL
                     KPFKEQIEGFQKRVNEVHSESLKGSANLEAEIKRVLQIGVSMSEEAQNLATALKGNNK
                     IAGNWGEVQLESALQSAGLLAGEHYQAQESFRDEEGRRFAPDFVVHLPDQKHLIIDSK
                     VSLVAYDQAVRSEENFAISQALDEHCRSLRNHIEGLSKKNYSALLGIKSPDFVLMFIP
                     IEPAYIEAMKHDPQLFNFGYERNVILVSHTTLMPILRTVANLWRIERGNSEAREISEK
                     AGEIYNQVCAVADRLAKLGNTLNTANNQYNQTVTSLVGRQGLLGKVERFQHLSSKASQ
                     IVPQVELIENEV"
BASE COUNT          616 a          354 c          413 g          460 t
ORIGIN      
        1 ttttttaacc ggcttttcga gattcgattt gcaaaaaaat tcaaaaaaaa gaccgcttgc
       61 gtgagcctgt tctagcggaa aaataagtgg ctttttctgc tcaaaatcaa ttaaaaagaa
      121 aaaagggcgt tttgcttgcc cgtagtgatt ggcttggtga ataaaatctt gcatagaaaa
      181 cacaatggct gaaattggct gaatcatcaa aagctctgtt ataatgcaaa gcaatagatt
      241 tgaaaagcat tttttaaaaa aggttttacc gatgaacaac accattcatt ttcaagaaat
      301 tgggatctac atcgcccttg ccgtttgtgt gctggtaggc gtttatctct ttttcttgaa
      361 atcccgttat cagcgagatg cctacgaatt aagccaagat cttggcaaaa tcaccgaaaa
      421 ttttgaacaa attcagcaaa aatatgaggt gttggctcaa gaaaaaaatc agcttgagca
      481 gtgggcaatt caacaacaga ccaaatatga agcagtgagt gaacgtttaa acgagcgaga
      541 tgggcaattg caacgattcc aacaaaaaat cgaacttgcc gaacagcaag aaaaccagct
      601 ggaacgctat attaatgagc taaaagaacg tgtgggttct tcccaagcaa aggcagaaag
      661 tttggaagaa cagctacaat ttagccaaag ttcactctca actaaagagc gggaaaatca
      721 agcgttattt agccgtttaa acgaggtaca aaacgagctg accgaactgc gtactacgct
      781 ttcggaaaaa caggcaaatt ttgaagcaca acaacgcaat tttattgagg taaaacaaca
      841 gcttaatgtg gaatttcaac atttggcaca acaaattctt gacgaaaaaa gcaagcgttt
      901 tagcgaaacc aatcaaagct cgttagaagc tctactgaag ccgttcaaag agcaaattga
      961 aggctttcaa aaacgggtga atgaggtaca tagtgagtcg ctcaaaggct cggcaaattt
     1021 agaggctgaa attaagcggg tgctacaaat tggcgtttca atgtcggaag aggcacaaaa
     1081 tctggcaacg gcattaaaag gcaataataa aattgccggc aactggggcg aagtacagct
     1141 tgaaagtgcc ttacaatctg ccggtttact cgcaggcgag cattaccaag cccaagagag
     1201 ttttcgtgat gaagaggggc gacgttttgc ccccgatttt gtggtgcatt tgccggatca
     1261 aaaacatctg attattgaca gtaaagtctc gttagtagcg tacgatcaag cggtcagatc
     1321 agaagaaaat tttgcaattt ctcaagcctt agacgaacat tgccgttcgc tacgcaatca
     1381 tattgaggga ttgtctaaga agaactacag tgcattatta ggcattaaaa gcccggattt
     1441 tgtactgatg tttattccga tagaaccggc ttatattgaa gcgatgaagc acgatccgca
     1501 actgtttaac tttggctatg aacgcaatgt gattttggtt tctcacacca ccttaatgcc
     1561 gattttacgc acggtcgcca atttatggcg gattgaacga ggcaacagcg aggctcgtga
     1621 aatcagcgaa aaagccggcg aaatttacaa ccaagtgtgt gcggttgccg atcggttagc
     1681 taaattaggt aacacgctaa ataccgcaaa taatcaatac aatcaaaccg ttacctcgtt
     1741 agtgggacgg caaggcttac tgggcaaagt ggaacgattc caacatcttt caagcaaagc
     1801 aagccaaatt gtgccgcagg ttgagctgat tgaaaatgag gtg
//
LOCUS       DANKBV010000222         1829 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.223, whole genome shotgun sequence.
ACCESSION   DANKBV010000222 DANKBV010000000
VERSION     DANKBV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.223"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(68..493)
                     /locus_tag="PWY43_002452"
     CDS             complement(68..493)
                     /locus_tag="PWY43_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2137275.1"
                     /translation="MYKHILVAVDLSEESLVLVRKGAALAEKCGAKLSLIHVDVNFSD
                     LYTGLIDINMSSVQDSVVEETNTALAELASKVNYPVSECLNGTGDFSQVLEEAVEKHN
                     VDLLITGHHQDFWSKFMSSTRQVMNNITVDMLVVPLADE"
     gene            724..1497
                     /locus_tag="PWY43_002453"
     CDS             724..1497
                     /locus_tag="PWY43_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HDL2137276.1"
                     /translation="MKIVSEPKTSGRHTCTIHCQNCSISQLCLPFTLNETELTQLDNI
                     IERKKPIQKSQVIFKSGDELRSLYAIRSGTLKAYTLSESGEEQITGFHLPGDLIGFDA
                     ITNMQHESYAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLL
                     SKMNAEEKLAAFLYNLSQRYSARGFSVKEFRLTMTRGDIGNYLGLTIETISRLLGRFQ
                     KSGMISVQGKYIIINRMEELAKLAGAIKPQQNIIAQTTA"
     gene            1641..>1829
                     /locus_tag="PWY43_002454"
     CDS             1641..>1829
                     /locus_tag="PWY43_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2137277.1"
                     /translation="MGILTGKRILVTGLASNRSIAYGIANAMKQQGAELAFTYLNDKL
                     KPRVEEFAKEFGSDIVLPL"
BASE COUNT          592 a          350 c          304 g          583 t
ORIGIN      
        1 ccaaaaaaat aaccgcttgt aagacagata aactgtctca agcggttatt tttcattcat
       61 tttcttgcta ttcatctgct aatggcacaa ccagcatatc aacagtgata ttattcatga
      121 cttgacgagt tgaagacata aatttactcc agaagtcctg atgatggcca gtgatgagta
      181 aatcgacatt gtgtttttct acggcctctt ccaaaacttg actaaaatct cccgttccgt
      241 ttaaacattc agatacagga taatttactt ttgaggcaag ttcagccagt gccgtatttg
      301 tttcttcaac aacactgtct tgtaccgaag acatattaat atcaattaat cctgtataga
      361 gatccgaaaa attaacatca acgtgaatga gggaaagttt agctccgcat ttttctgcta
      421 acgcagcccc cttacgcact aaaacaagac tttcttcaga aagatccact gcaactaaga
      481 tatgtttata cataatatgc tccttgcttg tgagagttgt ttactttgtg tataaacata
      541 ctaacacact ctttttgata aaaacttgaa gtagatctca aaaataaaaa tttgattata
      601 tttttcatac ctctttgtaa gtatttttat ttttccttgg tatactttgc ttcatgtaaa
      661 tttaatggta aactttgttc taaatcaaat ttaggaatac ttttctaatt taaggttgaa
      721 tggatgaaaa ttgtatcaga acctaaaaca agcggacgtc atacttgcac aattcattgt
      781 caaaattgca gtattagcca actatgctta ccttttactc ttaatgaaac ggaattaact
      841 caactagata atattattga gcgtaaaaaa ccgattcaaa aatcgcaagt tatttttaag
      901 tcaggtgatg aactgcgttc gctttatgct atccgttctg ggacattaaa agcctatacc
      961 ttaagtgaaa gtggtgaaga gcaaattacc ggtttccatt tacctggcga tctgattggc
     1021 tttgatgcaa ttaccaatat gcagcacgaa agctatgctc aagcactcga aacctcaatg
     1081 atttgtgaga ttccattcga tattttagat gatcttgccg gcaaaatgcc aaaaattcgc
     1141 catcaaatta tgcgtttgat gagtaatgaa attaaaagcg atcaagaaat gattctattg
     1201 ctctcaaaaa tgaatgcgga agaaaaacta gctgcatttt tgtataatct ttcccaacgt
     1261 tactcagccc gtggtttctc tgtaaaagag ttccgtctaa ctatgacccg tggagatatc
     1321 ggcaattatc ttggtttaac cattgaaact atcagccgtt tattagggcg tttccaaaaa
     1381 agcggtatga tttcagtaca aggaaaatat attattatta accgtatgga agaattagcc
     1441 aaattagccg gggcaatcaa accgcagcaa aatattattg ctcaaactac cgcttaattt
     1501 atcttaccaa atcccttttt gagacctcgt tcaaaaaggg atttctgatc cgatgagccg
     1561 ctaactccat ttgcccgttt ttcctatatc cgttataatc gacacaattt tgccccaatc
     1621 aatataaaag aggaattttt atgggtatct taactggtaa acgtatttta gtgacaggtt
     1681 tagcaagcaa ccgttcgatc gcttacggta ttgcaaacgc aatgaagcaa caaggtgcag
     1741 aacttgcatt cacctactta aacgataaat taaaaccacg agttgaagaa tttgccaaag
     1801 aatttggctc agacattgtc cttccgtta
//
LOCUS       DANKBV010000223         1807 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.224, whole genome shotgun sequence.
ACCESSION   DANKBV010000223 DANKBV010000000
VERSION     DANKBV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.224"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            70..561
                     /gene="tsaE"
                     /locus_tag="PWY43_002455"
     CDS             70..561
                     /gene="tsaE"
                     /locus_tag="PWY43_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL2137278.1"
                     /translation="MTDSLSFYCEDETKLLEFGQSFALELKKYLLQDQAHSLVVYLNG
                     ELGAGKTTLTRSIVRAFGHQGNVKSPTYTLVEEYQLPPFSLYHFDLYRLADPEELEFM
                     GIRDYFKPQTLCLLEWAVKGKGMIPEADFVIQIDYKNDGRQISLLPQNQTAVDILINF
                     HQK"
     gene            581..1777
                     /locus_tag="PWY43_002456"
     CDS             581..1777
                     /locus_tag="PWY43_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2137279.1"
                     /translation="MKQVIRFIVFSTLSLFSFSTFAKTIVAIDAGHGGKDPGAIGKNL
                     GILEKSVTLAISKELKALLDADPNFKAVMTRSGDYFIQLPQRTEIARKHRADLLVSIH
                     ADSSPRGDSLKGASVWVLSNSRASDEMGKWLEDHEKQSELLGGAGSVLSSNNERYLNQ
                     TVLDLQFSHSQRAGYELGKSILAKMSNVAALAKSSPQHASLSVLRSPDIPSVLVETGF
                     LSNSTEEAKLASANYRKQVARAIYNGLVAYRAKNAVASSSVSKKSDKEDKQNKKEEKT
                     AKADLTKKPASEKQEKNTQSDKTKLKEKSKKEDKKEDSAKTNKKKEQQLKEKTALEKD
                     QKNQSKKSDSQREIKVSNSGYHIVQQDETVYSIARAYGTTPQKISELNNLKNYQITVG
                     KKLKVK"
BASE COUNT          606 a          326 c          391 g          484 t
ORIGIN      
        1 cgatattcgt gaatccatta acgaattgaa attttatcgg gaacatttta ttcagctaga
       61 ccaaaaataa tgacagattc tctctctttt tactgcgaag atgaaactaa attgctggaa
      121 tttggtcaat cattcgcgtt agaacttaag aaatatttat tgcaagacca agctcattcg
      181 ctggttgttt acctaaacgg cgaacttggg gcaggtaaaa ccaccttaac tcgctcgatc
      241 gtacgggctt tcgggcatca aggcaatgta aaaagcccaa cttatactct ggtggaagaa
      301 taccaacttc cgcctttcag cctttaccat tttgatttgt atcgcttagc cgatcctgaa
      361 gagctggagt ttatgggaat tcgtgattat ttcaaaccac aaacgctttg tttgcttgaa
      421 tgggcagtta aaggtaaagg aatgattcca gaggctgatt ttgtgatcca aattgattat
      481 aagaatgacg gacggcagat tagcctgttg ccgcaaaatc aaactgcagt agatatatta
      541 ataaattttc atcaaaaata gaccgcttgt agggaaggta atgaagcaag ttatcagatt
      601 tattgttttt agcacattga gtttattcag tttttccaca tttgccaaaa ccattgtggc
      661 gattgatgcc ggacacgggg gaaaagatcc gggggctatt ggtaaaaatc tggggatttt
      721 ggaaaaaagc gttaccctcg caatttctaa agagctaaaa gccttgttag atgccgatcc
      781 taattttaaa gcagtgatga cacgctcggg agattatttt atccaattac cgcaacgtac
      841 cgagattgcc cgtaagcatc gtgccgatct gctggtttct attcacgcag actcttcgcc
      901 tcgtggcgat tccttgaaag gggcttcggt ttgggtactt tctaatagcc gtgctagcga
      961 tgaaatgggt aaatggttgg aagaccacga gaagcagtcg gaattacttg gcggggctgg
     1021 ttcggtgctt tcaagcaaca atgaacgtta tttaaatcaa acagtgcttg atttacagtt
     1081 ctctcactct caacgtgccg gctatgaatt aggcaagagc attttagcta aaatgtcaaa
     1141 tgtggctgca ttagcaaaat cgtctcctca gcacgcaagc ttaagtgttc tgcgttcacc
     1201 tgatattcca tctgtattgg tggaaacggg atttttatct aattcaaccg aggaagcaaa
     1261 gctagccagt gcaaattatc gtaaacaagt tgctcgggcc atttataatg gattagtcgc
     1321 ttatcgggca aaaaatgcag ttgctagttc ttcggtcagt aaaaaatcgg acaaagaaga
     1381 taagcaaaat aagaaagagg aaaaaactgc taaagctgat ttaactaaaa agcctgcttc
     1441 tgagaaacaa gagaagaata cgcaatcaga taagacaaaa ttgaaagaaa agagcaaaaa
     1501 agaggataaa aaagaagatt cggctaaaac caataagaaa aaagagcagc aactaaaaga
     1561 aaaaacagct cttgagaaag atcaaaaaaa tcagtctaaa aaatcagact ctcaaagaga
     1621 aattaaggtg tctaacagtg gctaccatat tgtccaacaa gatgaaacgg tttactcgat
     1681 tgctagagct tacggtacta cgccgcaaaa aatcagtgag ctgaataatt tgaaaaatta
     1741 tcagatcacg gtaggtaaaa aattgaaggt gaagtaaatt gaaaggctga tggaaatatc
     1801 catcagc
//
LOCUS       DANKBV010000224         1754 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.225, whole genome shotgun sequence.
ACCESSION   DANKBV010000224 DANKBV010000000
VERSION     DANKBV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.225"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>1754
                     /locus_tag="PWY43_002457"
     CDS             <1..>1754
                     /locus_tag="PWY43_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL2137280.1"
                     /translation="NIGGRNLLRDSEFNAYNKWGNPQIDFTENANRRTIKVTSTGTNG
                     PVGIVSSNRHSTSYFQQGETYTLSLFARGSKALDYLYLMRQDGNNVRLPVINVASETE
                     FNHYKLTFKAPFTTQQGYVLIGFRQTSTEQFVEFYSVKLEKGNVATDWTPAPEDVDSA
                     VSAVSADLTSYKQTQAATDSAQAQQLNQLSVNLTKAETNFNAKITEEKKARVDADKAN
                     AERLTDITSRVANAESTITNFQSTKANKSEVASLAQSSLQAVWKADTQSAIGELAVGG
                     RNLLLKSNATNLINFVRSSQDVLDNTDFKTPVVRINCIQDSWFGRKTAITSGKLLAGK
                     YTLSFKYRTNATINNTFIGYGGNQRRRLITSNVVGNETWQTCKLTFETTEDYPDIFII
                     IGGYGKADVSYIEFAELKLEKGAIATDWTPAPEDVESSVSAVSAKVDSVQQTLANADS
                     ALSSRIDTVNASVGSNSSKITQVSNAVASVDGKLSATHTIKTETIAGGRKAIAGIAFG
                     ATADNRNAESSVIVMADKFEVVKNAQDGTPVRLFGVVENKVAINGNLIATGSISGDHI
                     NANSVRTAVLTAGAIKTE"
BASE COUNT          576 a          351 c          384 g          443 t
ORIGIN      
        1 aatatcggag gtcggaattt attaagagac agcgagttca atgcgtacaa taaatggggt
       61 aatccacaga ttgattttac agaaaatgca aatcgtcgaa ctatcaaagt aacctcaaca
      121 ggtactaacg gtcctgttgg tattgtttca tctaatcgcc attccacatc ttatttccaa
      181 caaggagaaa cttatacact ctcattattt gctcgaggaa gtaaggcact agactatctc
      241 tatctaatgc gtcaagatgg gaataacgtt agattaccag tgattaacgt tgcctctgaa
      301 actgagttta atcattacaa actcacgttc aaggctcctt ttaccacgca acaaggttat
      361 gtcttaatcg gttttcggca aaccagcaca gagcagtttg ttgagtttta cagcgtcaaa
      421 cttgaaaaag gcaacgtagc cacagactgg acgccagcac ctgaagatgt ggacagtgcg
      481 gttagtgctg tttcggctga tttaacgagt tataaacaaa ctcaagcggc taccgattca
      541 gcacaagcac agcaacttaa tcagttatct gtgaacttga ccaaagcaga gactaatttc
      601 aacgccaaaa tcacggaaga aaaaaaagct cgtgttgatg cagataaagc gaatgcagag
      661 aggctcacag acataacaag ccgtgttgca aatgcggaat caactattac caatttccaa
      721 tcaaccaaag cgaataaaag tgaggttgcg agccttgcac aaagttcgtt gcaggcagtt
      781 tggaaggcag atacacaatc agcgattggc gagttggctg tcggtggaag aaatttatta
      841 ttaaaatcca acgcaacaaa cttaatcaat tttgtcagat catctcaaga tgtattagat
      901 aacactgatt tcaaaactcc agtagtacga atcaactgta tacaagatag ttggtttggt
      961 cgaaaaacag cgattacatc gggaaaattg cttgctggta aatacactct atcgtttaag
     1021 tatcgcacca acgctacaat taataatact tttattggtt atggaggcaa tcagcgcaga
     1081 cggcttatca catcaaatgt tgtcggtaat gagacatggc aaacttgcaa gctcacattt
     1141 gagacaaccg aggattaccc tgatatattt atcattattg gtggttatgg taaggcggat
     1201 gtaagttata ttgaatttgc cgagctgaaa ctagagaaag gcgctatcgc tacagattgg
     1261 acaccagcac cggaagatgt ggaaagttct gtaagtgcag tatctgcaaa agtagattcg
     1321 gttcaacaaa cgctggcaaa tgcagattca gcactaagct ctcgcattga tacggtcaat
     1381 gcctcagtcg gatcaaactc atctaaaatt acccaagtca gcaatgcggt agcaagtgtg
     1441 gacggcaaat tatctgctac ccataccatc aaaaccgaaa ccattgccgg tggacgcaaa
     1501 gcgattgccg gtattgcatt tggtgcaacg gcagataacc gtaatgcgga atcttctgtg
     1561 attgtgatgg cagataaatt tgaggtcgtg aaaaacgccc aagacggcac gccagtaaga
     1621 ctatttggcg tggtagaaaa taaagtagcg attaatggga atttaatcgc aacaggctcg
     1681 atttcaggag accacatcaa tgcaaattcc gtcagaactg ctgtattgac tgcaggagcg
     1741 attaaaacgg agca
//
LOCUS       DANKBV010000225         1691 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.226, whole genome shotgun sequence.
ACCESSION   DANKBV010000225 DANKBV010000000
VERSION     DANKBV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.226"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(73..1566)
                     /gene="mltF"
                     /locus_tag="PWY43_002458"
     CDS             complement(73..1566)
                     /gene="mltF"
                     /locus_tag="PWY43_002458"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDL2137281.1"
                     /translation="MKGLIARLFIAVALLLWAWDMIFPWQKIIHSEANHYTQIQQSKT
                     LKVGMINHPLSYFVGPEGKSGIEYDLATAFAQYLKVNLDIKTYDSSEKLFEALRNNDV
                     DIAAAGLLYHSELNDEFQIGPSYYSASWQLVYKKGSARPYRLSDLRENVIIPAGSAVI
                     PILNQLKVDNPELKWQISDKFTQEELLLKVAQGEISHTIAMSVDISAAQHINPNLAVG
                     FDLTDENPVLWYLANSSYSELQSVLLDFMSYSMETGLISRIEEKYFNHLAKFDYVDIQ
                     SYLNAIKTILPKYEPLFRAHKGELEWQMLAAIAYQESHWDPNATSPTGVRGIMMLTKD
                     TADRMKISDRTNPAQSIKAGSEYLHMLMRQMPETIPQADRIWYSLAAYNMGLGHLLDL
                     RRLTKQLGGDPDNWLDVKKNLPLLAEKRYYANLKYGYARGYEALQYVENIRRYYGSII
                     NYQRVEEQKMQEQENLPDGQIKQENSSYEKIEEKEIPTDKEITPASN"
BASE COUNT          528 a          336 c          259 g          568 t
ORIGIN      
        1 cagatggcct gttgcatctg cataaatgcc tttctacgtg cagaaattaa tctttttcta
       61 tctttaattt gcttaatttg acgcaggcgt aatttccttg tcggtaggga tttctttttc
      121 ttcaattttc tcataagaag aattctcctg ttttatttga ccatctggta aattttcttg
      181 ttcttgcatc ttttgctctt caactcgttg ataattaata atactaccat aataacgccg
      241 tatattttca acatactgta atgcttcata tcctcgagca tagccgtatt ttagattagc
      301 ataataacgt ttttccgcaa gcaatggcag attcttttta acatcaagcc aattatccgg
      361 atcaccacca agttgttttg ttaatcgacg aagatccaac aagtgcccca atcccatatt
      421 atacgctgct aggctatacc aaattctatc tgcttgagga atagtttccg gcatttgtct
      481 cattagcata tgtaaatatt cagatcccgc tttgatactt tgtgcagggt tagtcctatc
      541 agaaattttc attctatctg ccgtatcttt tgtcaacatc ataattcccc tcactccggt
      601 tggagaagtt gcattaggat cccaatgaga ctcctgatag gcgatagccg ccagcatttg
      661 ccattctaat tcacctttat gtgcccgaaa aaggggttca tatttaggta aaatagtttt
      721 aattgcattc aaatagctct gaatatcaac ataatcaaac ttagctaaat gattaaaata
      781 tttctcttct attcttgata tcagccccgt ttccatagaa taagacataa aatccagcag
      841 cacagattgc agttcactat aagaactatt tgctaaatac cataacactg ggttttcatc
      901 agttaaatca aaccctacgg ctaaattagg attaatatgc tgtgcagcag aaatatcaac
      961 cgacattgca attgtatgtg aaatttcacc ttgtgcgact tttaacaata gctcttcttg
     1021 tgtaaattta tccgagatct gccattttag ttccggatta tccaccttaa gctgatttaa
     1081 tattggaatt accgcagaac cggctggaat aatcacattt tcccgcaaat cggaaagtcg
     1141 atatggtcta gcacttccct ttttataaac aagctgccaa gaagccgaat aataggaagg
     1201 acctatctga aattcatcgt ttaattcaga atggtaaagc agcccagcgg cagcaatatc
     1261 aacatcatta tttcttaagg cctcaaacaa tttctcgcta ctatcataag ttttaatatc
     1321 aagattgacc tttaaatatt gagcaaaggc tgttgctaaa tcatattcaa tgccactttt
     1381 tccttcagga ccaacaaagt aagaaagtgg atggttgatc attccaacct ttaacgtttt
     1441 gctttgctga atttgtgtat aatgatttgc ttctgaatga atgatttttt gccaaggaaa
     1501 aatcatatcc caagcccaca gtaacaatgc tacggcaata aaaaggcgag caattaatcc
     1561 tttcaattta aaatcctcat tatttcaagc taagcattaa gcctatctga aattacaaat
     1621 taacggaata atactttata gtttaattat ttattaaaaa aataaaatgg gaatataatg
     1681 gatataatat a
//
LOCUS       DANKBV010000226         1658 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.227, whole genome shotgun sequence.
ACCESSION   DANKBV010000226 DANKBV010000000
VERSION     DANKBV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.227"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1306
                     /locus_tag="PWY43_002459"
     CDS             <1..1306
                     /locus_tag="PWY43_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL2137282.1"
                     /translation="ETLSKVKQNLIIQGDNLSALKSLLPFYAGQVKCIFIDPPYNTKS
                     AFTHYDDNLEHSVWLSMMYPRLEILRDLLAEDGSIWITLDDNESHYLKIMLDEIFGRK
                     NFISNLIWEKKRKPSFLGKVGKVTDNLFCYAKNKNELNDLTYLETSGDETYPLYNAGN
                     SREILVFPPRSIKFKKHSDGLYKPTIFSKKTSSITLISELIIEDQWNKNTVEIEGEWR
                     YSQKSIDEQLKNGDYYIIKSLDFRPRRVFERSAEKKKIHNLISRAHYDMATNEDSASE
                     HLELFGEDIAEDSFDYPKPEKLIQFVLEASTEPYDLVLDSFLGSGTTAAVAHKTNRRY
                     IGIEIGEHAKTHVVPRLKKVIEGEQGGISKAVNWQGGGSFRFCELGEEVFDAFGSLNP
                     NIRFDDLAAHIWYLENHFPLQKNSEKSPLVGTFNGKAYYFRR"
     gene            1263..1490
                     /locus_tag="PWY43_002460"
     CDS             1263..1490
                     /locus_tag="PWY43_002460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137283.1"
                     /translation="MSALSTAKPTILGDKRPQSGNVLTRKLMAELPYFAEFIQQGMDI
                     VVYGEACRLGEVQLAEKKITFKQIPYDVMSR"
     gene            1506..>1658
                     /locus_tag="PWY43_002461"
     CDS             1506..>1658
                     /locus_tag="PWY43_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HDL2137284.1"
                     /translation="MKLKNYQNEALNRVSHYFSACLQSDAKTAFSQIQPEYSYKIPSE
                     HSNLRDV"
BASE COUNT          569 a          267 c          320 g          502 t
ORIGIN      
        1 tgaaacctta tctaaagtca agcaaaattt aattattcag ggtgataatt tatcagcctt
       61 aaaatctctt ttgccctttt atgcggggca agtcaaatgt atttttattg atcctcccta
      121 taacacaaaa tcggccttta cccactacga tgataattta gagcattccg tttggctttc
      181 aatgatgtat ccacgccttg aaattctgcg agatttactt gccgaagatg gttcaatttg
      241 gatcactctt gatgataacg aaagtcacta tttgaaaatt atgcttgatg aaatttttgg
      301 gcggaagaat ttcatttcta acttaatttg ggaaaagaaa aggaaaccat cttttttagg
      361 caaagttggt aaagttacag ataatttatt ttgctatgca aaaaacaaaa atgaattaaa
      421 tgacttaact taccttgaga cctcaggtga tgaaacctat ccattataca atgctggaaa
      481 ttcaagagaa attttagttt tccctcctcg ttctataaaa tttaaaaaac atagtgatgg
      541 attatataag ccaaccattt tttctaaaaa aacgagttca attacgttga tttcggaatt
      601 aattattgaa gatcaatgga ataaaaatac agtagaaatt gaaggtgaat ggcggtatag
      661 ccaaaaaagt attgatgagc aattaaaaaa tggtgattac tatattataa aaagtttaga
      721 ctttcgacca aggagagtat ttgagcgaag tgcggaaaag aaaaaaattc ataacttaat
      781 tagcagagct cattatgata tggcaacaaa tgaagactct gctagtgaac atctagaatt
      841 atttggggag gatattgcag aagattcatt tgattatcct aaacctgaaa aattaatcca
      901 atttgtatta gaagcatcga ctgaaccata tgatctcgtc ctcgacagct ttcttggctc
      961 aggtacaact gcagcagttg ctcataaaac gaaccgtcgt tatatcggta ttgagattgg
     1021 cgaacatgct aaaacccatg ttgtcccacg gttgaaaaaa gtaattgaag gtgagcaagg
     1081 cggtatttca aaagcagtaa attggcaagg tggtggatcg tttcgctttt gtgagttagg
     1141 cgaggaagta tttgacgcat tcggttcact taatccgaac attcgttttg acgatttagc
     1201 cgcccatatt tggtatttag aaaatcattt ccctttgcaa aaaaacagcg aaaaatcacc
     1261 gcttgtcggc actttcaacg gcaaagccta ctattttagg cgataaacgt ccgcagagtg
     1321 gtaatgttct gacacgaaaa ttaatggcgg agttaccata ctttgctgaa tttattcaac
     1381 agggaatgga tattgtggta tatggcgaag cgtgccgttt aggagaagtg cagttagctg
     1441 aaaagaaaat tacatttaaa cagattccat atgatgtaat gtctcgttaa tagtaggaga
     1501 aaaaaatgaa attaaaaaat taccaaaatg aggcattaaa ccgtgtcagc cactattttt
     1561 cggcttgttt gcaatctgat gcaaaaaccg catttagcca aattcaacct gaatatagtt
     1621 acaagatccc aagtgaacac agtaatttgc gtgatgtg
//
LOCUS       DANKBV010000227         1578 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.228, whole genome shotgun sequence.
ACCESSION   DANKBV010000227 DANKBV010000000
VERSION     DANKBV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.228"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..576)
                     /locus_tag="PWY43_002462"
     CDS             complement(<1..576)
                     /locus_tag="PWY43_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDL2137285.1"
                     /translation="MNFTTYFSDENKRRSLILLSLFHIFIIAISNYLVQISFEVQVPL
                     TEFSFPTTWGTLTFPFIFLATDLTVRVFGADLARKIIFVVMFPALVISYVISVLFFET
                     QFQGFSALTEFNFFVFRIALASFAAYLVGQLLDVLVFNSLRQGKRWWLAPTCSTIFGT
                     LIDTFVFFAVAFYKSSDEYMAEHWFTIGSVDY"
     gene            769..1458
                     /locus_tag="PWY43_002463"
     CDS             769..1458
                     /locus_tag="PWY43_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDL2137286.1"
                     /translation="MLQLRKANERGDGSFSWLKSYHTFSFANYYDLKHIHFSHLRVIN
                     EDFIAPNHGFGKHPHDNMEILTYMLKGRVAHEDSMGNKTEVKAGEFQIMSAGTGVFHS
                     EINPDPNETLHLYQIWIIPNQKDVTPRYNQDRFAPKEGATLILSPNAEEGSFKIYQDM
                     KLWRYQYPTAKSEQLALEPNRSYWLQVVKGNLNINGVELSTSDALGIRQEKALEINTQ
                     DDVEFLLFDLV"
BASE COUNT          578 a          296 c          277 g          427 t
ORIGIN      
        1 ataatcgacc gaaccaatag taaaccaatg ctctgccata tattcatcgc tacttttata
       61 aaaggcgaca gcaaagaata caaaagtatc gattaaagta ccaaaaatgg ttgaacaggt
      121 tggagcaagc caccaacgct taccttgtct taaactatta aatactagaa cgtctaataa
      181 ttgcccaact aaataagctg caaagctagc cagtgcaata cggaaaacaa aaaagttgaa
      241 ctcagttaat gcactaaagc cttgaaattg ggtttcaaag aataaaacgg aaatcacata
      301 gcttatgaca agtgccggaa acatcacaac aaaaataatt tttctagcaa gatctgcacc
      361 aaatacacgc acggttaaat cggtcgctaa gaaaataaat gggaatgtta acgttcccca
      421 agttgtcgga aagctaaatt ctgttagtgg tacttgaact tcaaagctaa tttgcactaa
      481 atagttgcta attgcgatga taaaaatatg gaaaagtgaa agtaaaatta gagaacgacg
      541 tttgttctca tccgaaaaat aagtagtaaa gttcatattg tatccttttt cgttaaatga
      601 aaacttgagg taagggaact caagggggca ttattctata cattttccca taaaaatcaa
      661 gctcatatta tttcaaaaaa tgaaacaatt aatctattaa attagggttt atctattttt
      721 gacataaagt aaaatggcac aacttaacac ttacataagg aatagataat gttacaactg
      781 agaaaagcca atgaacgagg cgatggttct tttagctggc ttaaaagcta ccacactttt
      841 tcctttgcca attattacga cctaaaacat atccattttt cacatttacg ggtaattaac
      901 gaagatttca ttgcacctaa tcacggtttt ggcaagcacc cgcacgataa tatggaaatt
      961 ttaacttata tgttaaaagg acgtgtggct cacgaagaca gtatgggtaa taaaaccgaa
     1021 gtaaaagctg gcgaatttca aattatgtct gccggaaccg gggttttcca ttcagaaatt
     1081 aatccagatc cgaatgaaac cttacacctt taccagattt ggattatccc aaaccaaaaa
     1141 gatgtgaccc ctcgctacaa ccaagataga tttgccccga aagagggagc aaccttgatt
     1201 ctttcgccaa acgcagaaga aggttcattt aaaatttacc aagatatgaa gctgtggcgt
     1261 tatcaatacc caacagccaa atcagagcaa ctggcattag agccaaaccg cagttactgg
     1321 ctacaagtgg tgaaagggaa tctaaacatc aatggcgttg aactttccac cagcgatgcc
     1381 ttaggcattc gtcaagagaa agcacttgaa ataaacactc aagatgacgt ggaattttta
     1441 ctgtttgatc tagtgtaaat acaagagaca agcggttaaa tttgtaaaaa atattactaa
     1501 ttcagctgct tgctctcatt aaacattatc gcaccaaaat tctcactcct agcgtttcat
     1561 tttgttgaag taaggtat
//
LOCUS       DANKBV010000228         1551 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.229, whole genome shotgun sequence.
ACCESSION   DANKBV010000228 DANKBV010000000
VERSION     DANKBV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.229"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..310
                     /locus_tag="PWY43_002464"
     CDS             <1..310
                     /locus_tag="PWY43_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137287.1"
                     /translation="LHLDNNWANNTLEGKVTSKTLGEIGLDKTKLAPAVRLPTNEISF
                     YGTASATDHEGLKGEYSATFAGKTLNDAVGSIELENEDANNGDVSKYNAVFGVMKVEK
                     "
     gene            370..>1551
                     /locus_tag="PWY43_002465"
     CDS             370..>1551
                     /locus_tag="PWY43_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL2137288.1"
                     /translation="MKKIALLAFFPTLLFARDPNTTPAHLEHTAQPTTAPQAVAIEQS
                     PPIFNEAELRANQALTEELLTQAIYSRQLANMEKLTAIYQDFTQIDPILLRYAQGKIS
                     LLKGDYAQAIQHFQTILAKQPKLNTVRIELAIALLYDQQLNQAQAQFEKAKSAEHLPP
                     KVHALIEQYQQHLAKKQEWKFSFSANYVRDGNINRTSKQPNIEQTGYVKQPNLLPQTA
                     HGVHYAFGLQRDFNLFDSHYVSFDNQTQGELYWDNHDYDEITNRTLFGYAYKKSDQTT
                     RLLPFYEKRWKHHRSERWTNGIRLEHAQWFSPHWQLATALEYGKKRYFDLPIYNGSYK
                     LASATLLWLRNPQQFFFVGTDFNQDKTQLKQYSSDTKGIRFGWQREWQALELSSRLTL
                     GY"
BASE COUNT          507 a          370 c          293 g          381 t
ORIGIN      
        1 cctacactta gataacaatt gggcaaataa tacgttagaa ggtaaggtaa ccagtaaaac
       61 actgggtgaa attgggctgg ataaaaccaa actcgcacct gccgtaagat taccgactaa
      121 tgaaatcagc ttctacggca ccgcaagtgc tactgaccac gagggtttaa aaggcgaata
      181 ttcagcaacc tttgccggta aaacactcaa tgatgccgta ggttcaattg aattggaaaa
      241 tgaagatgcc aataacggtg acgtcagcaa atataacgcc gtcttcggtg taatgaaagt
      301 tgaaaaataa cctcatatta cgataatcat agagggctgg caacagccct ttgtttttaa
      361 ggacgaacaa tgaaaaaaat tgctttgctg gcttttttcc cgacactact ctttgcccga
      421 gatccgaata cgacaccagc tcatcttgag catacagcac agccaacaac cgcaccacaa
      481 gctgttgcga ttgaacaatc cccccccatt tttaatgaag cggaattacg ggcaaatcag
      541 gcattaaccg aggagctgct gactcaagcc atttatagcc gtcaattagc caatatggaa
      601 aaattgaccg ctatttatca agattttacg cagattgacc ctattttact gcgttatgca
      661 caaggtaaaa tttccctact gaaaggcgat tacgcacagg caattcagca cttccaaacc
      721 attcttgcca aacaacccaa actaaacact gtaagaattg agctggcgat tgctctattg
      781 tacgatcagc aacttaatca agcccaagca caatttgaaa aagccaaaag tgccgaacat
      841 ctaccgccta aagttcacgc attaattgag caatatcagc aacatctggc aaaaaaacag
      901 gaatggaaat ttagtttttc agccaattat gtgcgagacg gcaacattaa ccgaacctcg
      961 aaacagccga acattgagca aaccggctat gtaaaacaac ctaatttgtt gccccaaacc
     1021 gcacatggtg ttcactatgc gtttggatta cagagagatt tcaacctgtt cgactcacac
     1081 tatgtgagtt ttgacaacca aacccaaggt gaactttatt gggataacca cgattatgat
     1141 gaaattacca atcgcacctt gtttggatac gcttacaaaa aatccgacca aaccacccgc
     1201 ttgttaccgt tttatgaaaa acgctggaaa catcaccgtt cggaacgctg gacaaacgga
     1261 attcgacttg aacatgcaca atggttttca ccgcattggc aactcgccac cgcattggaa
     1321 tacggcaaaa aacgttattt tgatttgccg atttataacg gcagctacaa acttgcctct
     1381 gccacattgc tttggttacg caatccacaa caatttttct ttgtcggcac tgatttcaat
     1441 caagacaaaa ctcagttaaa acaatatagc tctgatacta aaggcatacg ctttggctgg
     1501 caacgagaat ggcaagccct cgagctttcc agtcgcctca cgcttggcta t
//
LOCUS       DANKBV010000229         1517 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.230, whole genome shotgun sequence.
ACCESSION   DANKBV010000229 DANKBV010000000
VERSION     DANKBV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.230"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1020)
                     /gene="rfbB"
                     /locus_tag="PWY43_002466"
     CDS             complement(<1..1020)
                     /gene="rfbB"
                     /locus_tag="PWY43_002466"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2137289.1"
                     /translation="MSLNVLITGGAGFIGSALIRFLAKQTDHHIINVDKLTYAANLAS
                     IKTVQNSERYYFEQVDICASGQISEIFCKYQPDAVMHLAAESHVDRSIKSAGEFIQTN
                     IVGTYSLLEVTRQYWEHLPEPRKSAFRFIHISTDEVYGDLPLTAEPSAENLPYAPSNP
                     YSASKASADHLVRAWYRTYGLPAIITNCSNNFGPYQYPEKLIPLMISNALQGKALPIY
                     GDGLQIRDWIFVEDHIRALYDVLIKGKLGASYNIGSHQEKSNIEVVYSICELLEELVP
                     NKPKNVKKYVDLIRYVQDRPGHDVRYALDSTKIQKELGWKPQETFESGLRKTVNWYLD
                     NKEWWV"
     gene            complement(1305..>1517)
                     /locus_tag="PWY43_002467"
     CDS             complement(1305..>1517)
                     /locus_tag="PWY43_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2137290.1"
                     /translation="QLLNVFKGEMSLIGPRPYMVIEKKDIGNKISLVLGVKPGITGLW
                     QVSGRSDTDFDTRVQMDVWYMKNWSL"
BASE COUNT          529 a          298 c          219 g          471 t
ORIGIN      
        1 aacccaccat tctttattat ctaaatacca atttactgtt ttacgaagac cactttcaaa
       61 tgtctcctga ggtttccagc ctaactcttt ttgaatttta gttgaatcta atgcgtagcg
      121 gacatcatgc ccgggccggt cttgaacata gcgaattaga tcaacatatt ttttaacatt
      181 cttaggctta tttggcacta actcctctaa cagctcacaa attgaataaa ccacctcaat
      241 attgcttttt tcttgatggg agccaatatt ataacttgcg cctaatttac ctttaattaa
      301 aacatcgtat aaagcacgaa tgtgatcttc gacgaaaatc caatcccgaa tttgcaaacc
      361 atcaccataa ataggcaaag cctttccttg caatgcattt gaaatcatta atgggatcaa
      421 tttttcagga tattgataag gaccaaaatt attggagcaa tttgtaataa tcgctggtaa
      481 accataagtt ctataccaag cacgtactaa atgatcagcc gatgctttgg aagcagaata
      541 agggttactt ggggcataag gtaaattttc agcagatggt tcagctgtta atggcaaatc
      601 accatacact tcatctgttg aaatgtggat aaaacgaaac gctgattttc gaggttcggg
      661 caaatgctcc caatattgtc gagtgacttc taacagagaa taagttccaa caatattagt
      721 ttgaataaac tcaccagcac tcttgattga gcgatcaaca tggctttcag cagccaaatg
      781 catcaccgca tcaggctgat atttgcaaaa aatctcggaa atttgaccac ttgcacaaat
      841 atcaacttgc tcaaaataat aacgctcaga attctgtacg gttttgattg aggctaaatt
      901 tgcagcataa gtgagcttat ctacattgat tatatgatgg tctgtttgct tagctaaaaa
      961 acgaattaac gcggagccga taaatccggc tccacctgta attaatacat ttaaactcat
     1021 catataacta ataggctaag gaaataaata tcttaaaaag gaattttgca aaaaaatatc
     1081 gcaatcacac gcttacaata tataaacata gataataaca taagacataa ttataataaa
     1141 aaagctaaag ccctaataat attcaaatat ataaatcttg aattaaatga ttagcttttt
     1201 agctttttaa cttattaagc ttatttaaaa atactattta tctaatatgc accatctctt
     1261 tttaaaactg ttttaaatgt tttaaacaaa atgacaatat cacctcacaa tgaccaattt
     1321 ttcatatacc aaacatccat ctgaactcga gtatcaaaat cagtatcact acggccgctt
     1381 acttgccata aacctgtaat tccgggctta acgcctaaaa ccaaggagat tttattacca
     1441 atatcttttt tctcaattac catataaggg cgaggtccta ttaagctcat ttccccttta
     1501 aaaacattta acaactg
//
LOCUS       DANKBV010000230         1515 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.231, whole genome shotgun sequence.
ACCESSION   DANKBV010000230 DANKBV010000000
VERSION     DANKBV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.231"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(25..>1515)
                     /locus_tag="PWY43_002468"
     rRNA            complement(25..>1515)
                     /locus_tag="PWY43_002468"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          320 a          475 c          320 g          400 t
ORIGIN      
        1 acttgctgtc gttctcgatt ttggtaagga ggtgatccaa ccgcaggttc ccctacggtt
       61 accttgttac gacttcaccc cagtcatgaa tcataccgtg gtaaacgccc ccctttcggt
      121 taagctatct acttctggta caacccactc ccatggtgtg acgggcggtg tgtacaaggc
      181 ccgggaacgt attcaccgca acattctgat ttgcgattac tagcgattcc gacttcatgg
      241 agtcgagttg cagactccaa tccggactta gacgtacttt ctgagattcg ctccccatcg
      301 ctggctcgcc gccctctgta tacgccattg tagcacgtgt gtagccctac tcgtaagggc
      361 catgatgact tgacgtcatc cccaccttcc tccggtttat caccggcagt ctcctttgag
      421 ttcccgacct aatcgctggc aacaaaggat aagggttgcg ctcgttgcgg gacttaaccc
      481 aacatttcac aacacgagct gacgacagcc atgcagcacc tgtctcatgg ttcccgaagg
      541 cactctcata tctctacaag attccatgga tgtcaagagt aggtaaggtt cttcgcgttg
      601 catcgaatta aaccacatgc tccaccgctt gtgcgggccc ccgtcaattc atttgagttt
      661 taaccttgcg gccgtactcc ccaggcggtc gatttatcac gttagctacg ggcaccagag
      721 ttaaacccca atccccaaat cgacagcgtt tacggcgtgg actaccaggg tatctaatcc
      781 tgtttgctcc ccacgctttc gcacatgagc gtcagtacat ttccaagggg ctgccttcgc
      841 cttcggtatt cctccacatc tctacgcatt tcaccgctac acgtggaatt ctacccctcc
      901 ctaaagtact ctagttgttc agtctgaaat gcaattccca ggttaagccc ggggctttca
      961 cacctcactt aaacaaccgc ctgcgtgccc tttacgccca gttattccga ttaacgctcg
     1021 caccccccgt attaccgcgg ctgctggcac ggagttagcc ggtgcttctt ctgtgattaa
     1081 cgtcaatcga ctgttctatt aaaacaatcg ccttcctcgt caccgaaaga actttacaac
     1141 ccgaaggcct tcttcattca cgcggcatgg ctgcatcagg gttcccccca ttgtgcaata
     1201 ttccccactg ctgcctcccg taggagtctg gaccgtgtct cagttccagt gtggctggtc
     1261 atcctctcag accagctaga gatcgcgggc ttggtgagcc tttacctcac caactaccta
     1321 atcccacttg ggctcatctt atggcaggtg gccaaatggt cccacccttt agtcctaaga
     1381 cattacgcgg tattagctac cgtttccagt agttatcccc ctccataagc cagattccca
     1441 agcattactc acccgtccgc cactcgtcac ccaaggagca aactcctatg tgctaccgtt
     1501 cgacttgcat gtgtt
//
LOCUS       DANKBV010000231         1507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.232, whole genome shotgun sequence.
ACCESSION   DANKBV010000231 DANKBV010000000
VERSION     DANKBV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.232"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..623
                     /locus_tag="PWY43_002469"
     CDS             <1..623
                     /locus_tag="PWY43_002469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDL2137291.1"
                     /translation="PYITQQLASFNLYKMYQAFPMLMDSRFNQIHFQSTQPIVNGELF
                     FRYIEHYRESYIFLFDKQKGFLTNSKIFSGKLRDYDNNLLRYIDEYTGAYRIGDKYIK
                     NLFKCLIILYYDKFGTENLIQAVEKCFKWSYRIRLMQSRILYRTIENEVHYMHSLFTH
                     LVRSDNSREFLEFMIDRFKFECYQDQKIGLSNLLEDLEKKDEQKTS"
     gene            604..>1507
                     /locus_tag="PWY43_002470"
     CDS             604..>1507
                     /locus_tag="PWY43_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDL2137292.1"
                     /translation="MNKKQVKTLYVKDLCNIDKYIVPIYQRNYAWGRGEIELLIQDIE
                     EAQKKEEIQKESEKHRYYIGSLVVSKRSDGYEVIDGQQRLTTLKLLLSYYNLKYSEQK
                     HINLTFEHREASNYSLSNLTNSSKESDSIRLGYQIISNILEQKANLSGKENLDIKEFF
                     TFLLNNVVILRTEVPEDTDLNHYFEIMNNRGEQLEHHEVLKATLMSSLKDYKEKKLFA
                     LIWDACSDMSVYAIKRFPSDIRTSNEFFGEHCHQIPLCFDNMRNKLSEVLERKSDEGN
                     GENTNKDEDDKKKIISLLNRKYVEN"
BASE COUNT          576 a          182 c          264 g          485 t
ORIGIN      
        1 atccttatat cacacaacaa cttgcaagtt ttaatttata taaaatgtat caggctttcc
       61 ctatgttaat ggatagtcga tttaatcaaa ttcactttca atcgactcaa cctattgtaa
      121 atggagaact ctttttccgt tatattgagc attatagaga aagctatatt ttcttatttg
      181 ataagcagaa aggttttttg acaaattcta agattttttc agggaaattg agagactatg
      241 ataataattt gttaaggtat attgatgaat ataccggagc ttatcgaatc ggtgataaat
      301 atattaaaaa tttatttaaa tgtttgataa ttctctacta tgataaattt ggtacggaaa
      361 atctcattca agccgtagaa aaatgcttta agtggagcta tcggattcgt ttaatgcaaa
      421 gtaggatatt atatcgaact atagaaaatg aagttcatta tatgcatagc ttatttactc
      481 atcttgtcag aagtgataat tcacgagaat ttctagaatt tatgatagat agatttaagt
      541 ttgagtgcta tcaagatcag aaaattggac taagtaactt actggaagat ttggagaaaa
      601 aagatgaaca aaaaacaagt taaaacacta tatgtaaaag atctatgtaa tattgataaa
      661 tatattgtac ctatctatca gcgtaactat gcttggggaa gaggtgaaat tgaattactg
      721 attcaagata ttgaagaagc tcagaaaaaa gaagaaattc agaaagaatc agaaaagcac
      781 cgttactata ttggtagttt agtagtatct aaacgatctg atggctacga agttattgat
      841 ggacaacaac gattaacgac tttaaaacta ttgttaagtt attacaatct aaaatattca
      901 gaacaaaagc atattaatct gacttttgaa catcgtgaag catctaacta cagcttatca
      961 aacctcacaa atagttcaaa agagagtgat agcatcaggt tgggttatca aattatctcc
     1021 aatatactgg aacagaaagc taacctatct ggaaaagaaa atttagatat taaagagttc
     1081 ttcacattct tgctaaataa tgttgttatt ctcagaacag aagtaccaga agatacggat
     1141 cttaatcatt attttgaaat tatgaataat cgtggtgaac agttagaaca tcacgaggtt
     1201 ttaaaagcaa cgctgatgtc ctcattgaag gattataaag aaaagaaact gtttgcttta
     1261 atttgggatg cttgctcgga tatgagtgtt tatgcgatta aacgctttcc tagtgatatt
     1321 agaacaagca atgaattttt tggagaacat tgtcatcaaa taccattatg ttttgataat
     1381 atgagaaata aattatcgga agttttagag aggaaatctg atgaaggtaa tggagaaaat
     1441 acgaataaag atgaagatga taagaaaaag attatttcgt tattaaatag aaagtatgtt
     1501 gaaaata
//
LOCUS       DANKBV010000232         1507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.233, whole genome shotgun sequence.
ACCESSION   DANKBV010000232 DANKBV010000000
VERSION     DANKBV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.233"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..220)
                     /locus_tag="PWY43_002471"
     CDS             complement(<1..220)
                     /locus_tag="PWY43_002471"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604033.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer of AccA2D2 subunits; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HDL2137293.1"
                     /translation="MSWLDRILGRDSSSSSSAKSKVPEGVWTKCTSCEQVLYSEELQR
                     NQQVCPKCDHHMRIDARTRLLNLLDKDSA"
     gene            530..1312
                     /gene="mazG"
                     /locus_tag="PWY43_002472"
     CDS             530..1312
                     /gene="mazG"
                     /locus_tag="PWY43_002472"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDL2137294.1"
                     /translation="MATIEQFLDVVNQLRHPEHGCPWDLKQNFDTMLPHLLEETYEVA
                     EAIHTQDRTALREELGDLLLQVVFLSQLAKEEGAFTFDDVVTDIHDKLIYRHPHVFGD
                     VKATNSEEALQSWEAQKANDAKHRSQDSILDDLPFALPALTRANKLQKRCAKVGFDWD
                     NPHDVLAKVEEELDEVKAEIAQYPARSAELAEELGDLLFAIVNLCRHYHTDAEEILRN
                     ANLKFEHRFRKVEKSVKKSGRTVKECKLEELDVIWHKIKVVE"
     gene            1380..>1507
                     /locus_tag="PWY43_002473"
     CDS             1380..>1507
                     /locus_tag="PWY43_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484215.1"
                     /note="response regulator in two-component regulatory
                     system with CpxA; part of the envelope stress response
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDL2137295.1"
                     /translation="MSKILLIDDDIEFTELLTELLSLEGFKMKVVHNGLEGLNELES"
BASE COUNT          488 a          262 c          296 g          461 t
ORIGIN      
        1 ccgcagaatc tttatctaac aaattcaata aacgggtgcg ggcatcaata cgcatatgat
       61 gatcgcattt tggacaaacc tgttggttgc gttgtaattc ttcgctatag agaacttgtt
      121 cacaacttgt gcattttgtc cacacgcctt ccggcacttt tgattttgca ctgcttgatg
      181 acgaagagtc tctacctaaa attctgtcta accagctcat tattattcct tttggattga
      241 gttgttgggt tcactaaatg gttgctatta aagcataatt tatcggtttt tgatatgttt
      301 tcttgacata tcagatcact aactatgtgt ataagttcga taaaaaaacg caatcagaag
      361 aatgattaac atcaccacaa tttgaattaa gcgttttttg gttaattttt ccgctgccat
      421 tgctctgctc atacttaata aatttccgca ttataagcga aaaagtacgg gctatggtag
      481 aatttgcaaa aatttgatca aaaataaccg cttgtaaaaa ggaaatacta tggcaaccat
      541 tgaacaattt cttgatgttg taaaccaact tcgccaccct gaacacggct gcccgtggga
      601 tctcaaacag aattttgaca cgatgttgcc ccacttgttg gaagaaacct atgaggtagc
      661 ggaagcgatt cacacccaag atcgcactgc tctgcgtgaa gaattgggtg atttattact
      721 gcaagtggtc tttttaagcc aattagcgaa agaagaagga gcttttacgt ttgatgatgt
      781 agtaaccgat attcacgata aactgattta ccgccaccct cacgtttttg gtgatgtgaa
      841 agctactaat agcgaagagg ccttacaaag ttgggaggca caaaaagcga atgatgcaaa
      901 acaccgtagc caagattcta ttttagacga tttacctttt gctttgcctg ctcttacccg
      961 tgcaaataag ctacaaaaac ggtgtgcgaa agtcggattc gattgggata acccgcacga
     1021 tgtgctggca aaagttgaag aagaactcga tgaagtcaaa gcagaaatcg cacaatatcc
     1081 ggctcgctcc gccgaacttg ctgaagaatt aggtgatctc ttgtttgcga ttgtaaattt
     1141 atgccgccat tatcataccg atgccgagga aattttacgc aatgcaaatc taaaatttga
     1201 acatcgcttt cgaaaagttg aaaaatctgt gaaaaaatcg ggaagaactg taaaagaatg
     1261 taaattagaa gaattagatg taatttggca caaaataaaa gtggtagaat aaaaaacatc
     1321 aattgttata ataattttta cctttgtaaa gttagtttta attttatagg aaatatttta
     1381 tgtcaaaaat cttattaata gatgatgata ttgagtttac tgagctacta actgaattac
     1441 tttcattaga aggatttaaa atgaaagttg tgcataatgg tttagaaggc ttaaatgagt
     1501 tagagtc
//
LOCUS       DANKBV010000233         1503 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.234, whole genome shotgun sequence.
ACCESSION   DANKBV010000233 DANKBV010000000
VERSION     DANKBV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.234"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..257)
                     /locus_tag="PWY43_002474"
     CDS             complement(<1..257)
                     /locus_tag="PWY43_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137296.1"
                     /translation="MSSIKEIFSTKNSPINVLMQTEWFLGFILIVVYLDAYFQLVHQV
                     PIYIAIQQKEIYLTKTIDYIAAIFFFSVTFTALFTMMRLLH"
     gene            complement(327..1460)
                     /locus_tag="PWY43_002475"
     CDS             complement(327..1460)
                     /locus_tag="PWY43_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2137297.1"
                     /translation="MKTIKIGLVGTGYIGRCHAIAYAQAPTLFPLKGKIQLEYLAEIN
                     QALADQKAREFGFQRATDDWRKLVTDPNVDVVDICTPNFLHKEIALEAIKHGKHVYSE
                     KPLALTAQDAKEMVIAAQQAGVKTLVGFNYIKNPTTQLARQIIQNGEIGEVVHFYGTH
                     NEDYLANPNTPIDWHCYKAKAGLGTLGDLAAHIVSIAHYLVGSDIERVIGDMQTVIEE
                     RPSPHNPAEKIKVENEDQASSLIRFANGVMGTLESSRISCGRKMGLTYVVTGTKGTLS
                     YTQERMAELKLYLHDDDKSRQGFKTILVGPEHPDYAAFCVSAGHGIGFNDQKAVEIRD
                     LINGIAADDPMYPDFEEGYKISRVLEAIAKSAEEKRWVEVSEI"
BASE COUNT          390 a          317 c          331 g          465 t
ORIGIN      
        1 atatgtaata aacgcatcat tgtaaataat gcggtaaatg taactgaaaa aaagaaaatt
       61 gctgcaatat aatcaatggt ttttgtgaga tatatttcct tttgctgtat cgcaatatag
      121 ataggcactt gatgaacaag ttggaagtaa gcatccaaat aaacaactat caatatgaag
      181 cctaaaaacc attctgtttg cataagaaca ttgatagggc tgttttttgt gctaaaaatt
      241 tctttaattg aactcataat ttttccttaa atgtaaaaaa acgtacctca accaatttga
      301 gatacgtttc tattttaatg taattattaa atctcactca cctccaccca ccttttctcc
      361 tccgccgatt ttgctatcgc ttccaatact ctggagattt tatagccttc ttcaaaatcg
      421 ggatacatcg ggtcatcagc ggcgatgccg ttgatgaggt cacggatttc caccgctttt
      481 tggtcgttaa aaccgatgcc gtgaccggca ctcacacaaa aagcagcgta gtcgggatgt
      541 tccgggccga ctaaaatggt tttgaatcct tggcgggatt tatcgtcatc gtgcaaatag
      601 agtttgagtt ctgccattcg ttcttgggta tagctgagcg tgcctttagt acctgtaacg
      661 acataggtca gccccatttt acgcccgcag gaaatgcggg agctttcgag cgtgcccatt
      721 accccattag caaatcgtat gagactactt gcctgatctt cgttttctac tttgattttt
      781 tctgctggat tgtgcggact agggcgttct tcaatcactg tttgcatatc gccgataaca
      841 cgctcaatgt ctgaaccaac caaatagtgt gcgatactga caatatgtgc ggctaaatct
      901 cccaacgtac caagcccggc tttggctttg tagcagtgcc aatcaatcgg ggtattaggg
      961 ttggcgagat aatcttcgtt atgcgtgccg tagaaatgca ccacctcacc aatttcgccg
     1021 ttttgaataa tttggcgagc cagttgggtg gttggatttt tgatatagtt gaaaccgacc
     1081 aaggttttaa ctcccgcttg ttgagcggca atcaccattt ctttggcatc ttgggcggtg
     1141 agtgcaagcg gtttttctga atagacgtgt ttgccgtgtt taatcgcttc taaggcaatt
     1201 tctttgtgca gaaaatttgg tgtacaaatg tccaccacat cgacattcgg atcggtaacc
     1261 aattttcgcc aatcgtcagt agcacgctga aagccaaatt ctcgggcttt ttgatcggct
     1321 aatgcttggt tgatttcggc aaggtattcc agttggattt tgcctttaag tggaaacagg
     1381 gttggggctt gagcgtaggc aatggcgtgg cagcgaccga tatagccggt gccaactaag
     1441 ccgattttga tggttttcat aatactatcc cagtagggtg cgtttgctaa cgcaccatta
     1501 gaa
//
LOCUS       DANKBV010000234         1491 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.235, whole genome shotgun sequence.
ACCESSION   DANKBV010000234 DANKBV010000000
VERSION     DANKBV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.235"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..493
                     /gene="ispE"
                     /locus_tag="PWY43_002476"
     CDS             <1..493
                     /gene="ispE"
                     /locus_tag="PWY43_002476"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2137298.1"
                     /translation="NTELSLEKLAELGLSLGADVPIFVRGMAAFAEGVGEVLTPCDPQ
                     EKWYVVLKPEVSISTEAVFTDPNLPRNTPKRPLAELLSLPWENDCEKVVREKYSAVED
                     LIQELLQYAQFRLTGTGACIFAEFETKAEAEQVFAHKPEAVFGFVAKGQNISPLHQML
                     FQI"
     gene            519..1469
                     /locus_tag="PWY43_002477"
     CDS             519..1469
                     /locus_tag="PWY43_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDL2137299.1"
                     /translation="MTDIKLFAGNATPELANRIAKHLYTSLGNATVGRFSDGEIQVQI
                     NENVRGGDIFIVQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVIADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLIDDILKKS
                     DLVNPIVVSPDIGGVVRARAVAKLLNDTDMAIIDKRRPKANVSQVMHIIGDVADRDCI
                     LVDDMIDTGGTLVKAAEALKERGARRVFAYATHAVFSGSAAQNLANPALDEIVVTDTI
                     PLSNEIKALDKVRVLTLSGMLAEAIRRISNEESISAMFDA"
BASE COUNT          432 a          278 c          343 g          438 t
ORIGIN      
        1 gaataccgag ctttcattgg aaaaattggc agaattaggc ttaagtttag gggctgatgt
       61 gccgatattt gttagaggta tggctgcttt tgccgaaggc gtgggggagg tgttaacccc
      121 ttgtgatcca caagaaaaat ggtatgtagt gctaaaacca gaagtgtcga tttcaaccga
      181 agcggtgttt accgacccga atttacctag aaatactcca aaacgcccct tagccgaatt
      241 attatcttta ccgtgggaaa acgattgcga aaaagttgta cgagaaaagt attctgcggt
      301 tgaagatctt atacaagaat tgttacaata tgctcagttt aggctaacag gcacaggtgc
      361 ttgtattttc gccgaatttg aaacaaaagc agaagccgag caggtttttg cacataaacc
      421 ggaagccgtc ttcggttttg tggcgaaagg gcaaaatatt tcgccattac atcaaatgtt
      481 attccaaatc taaccatctt aaccttaagg atcaatccat gacagatatt aaactgtttg
      541 cgggcaacgc aactccggaa cttgctaatc gtattgcaaa acatctttat acctcattag
      601 gtaatgcaac agttggtcgt tttagcgatg gtgaaattca agtacaaatc aatgaaaatg
      661 tgcgtggagg cgatattttt attgtgcaat ccacttgtgc accaactaat gataatttaa
      721 tggaattgat cgtgatggta gatgcgcttc gccgtgcatc agctggtcgt attaccgcag
      781 tgattccgta tttcggttat gcacgccaag acagacgcgt gcgttcagcc cgtgtgccaa
      841 ttaccgcaaa agtgattgct gatttcttat ccagcgtagg ggtagataga gtcttaacct
      901 gcgacttaca cgcagagcaa attcaaggtt tctttgatgt gccggtagat aacgtatttg
      961 gttcccctgt gttaatcgat gatattttga aaaaatccga tttggtcaac cctatcgtgg
     1021 tttctccaga tattggtggg gttgttcgtg caagagcggt ggcaaaatta ttaaatgata
     1081 ccgatatggc aattattgat aaacgtcgtc caaaagcgaa tgtatcacag gtaatgcaca
     1141 ttatcggtga tgtggctgat cgtgactgta ttttagttga tgatatgatc gatactggtg
     1201 gtactttagt caaagcggct gaagcgctga aagaacgtgg cgcccgtcgt gtctttgctt
     1261 atgccacaca cgcggtattt tcaggctcag cagctcaaaa tttagcgaat cctgctttag
     1321 atgaaattgt agtaactgat actattccac tgagtaatga gattaaagca ttagataaag
     1381 tacgtgtttt aacattatcc ggtatgttag cagaagcaat tcgccgtatt agcaatgaag
     1441 aatccatttc agcaatgttt gatgcctaat ttatgagata aaaacccagt c
//
LOCUS       DANKBV010000235         1466 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.236, whole genome shotgun sequence.
ACCESSION   DANKBV010000235 DANKBV010000000
VERSION     DANKBV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.236"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(124..384)
                     /locus_tag="PWY43_002478"
     CDS             complement(124..384)
                     /locus_tag="PWY43_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137300.1"
                     /translation="MPRNELTKNARAIVDLIHRKSATVTHKKLARAIGLSESQFSRTF
                     ADNVEMVAVIIDYLSIELADKDELLELKEKRYKRINLEEAKT"
     gene            complement(405..605)
                     /locus_tag="PWY43_002479"
     CDS             complement(405..605)
                     /locus_tag="PWY43_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2137301.1"
                     /translation="MNLTSYLSDRPRGFKADFAKKLGISKSYLRQVETGYSPMPAYLA
                     KKIEEVTNGEVAKSELRPDLWD"
     gene            736..1419
                     /locus_tag="PWY43_002480"
     CDS             736..1419
                     /locus_tag="PWY43_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2137302.1"
                     /translation="MIKGMTLPEIRRKKLTEWFNTRDIPEKEKSYISQLKSGKSSFGE
                     RAARRLENEYGMPSFYLDYDEETESAIVSAMSIQETQSHQYPIHLIDFKAKAGETGFI
                     NTNYPEIIQSIYFSLDGLLEIVGRKSSNGIEMITIPTDSMSPTINKGDVVFIDTTINY
                     YNNEGVYIFVIDDEVYIKRLQKIPGGIYRALSDNKTYEPFDIKRETLDTAIILGKFIR
                     VLPINPKDL"
BASE COUNT          481 a          254 c          258 g          473 t
ORIGIN      
        1 tttttaattg ttaaaaaccc accacgcaag cagcataaaa attgccactc taataaaaat
       61 tgaattttta ctgtggtaga atgatttgaa tttttctaaa aaatttttca ttgttggtaa
      121 cttctatgtt ttcgcttctt cgagatttat tcgcttatat cgtttctctt tcagctctag
      181 taactcgtct ttatccgcaa gctcaatact taagtaatca ataatcaccg ccaccatttc
      241 cacattatct gcaaatgtac ggctaaattg tgattcactc agtccgattg ctcttgctag
      301 tttcttatgc gtcaccgttg cggattttcg atgaatcaaa tccacaatcg ctcttgcatt
      361 ttttgttaat tcattgcgtg gcattgcaca ttcctttggg taaattaatc ccacagatcg
      421 gggcgaagct cagatttggc tacttcgcca ttcgtgactt cttcaatttt cttagcaaga
      481 taggctggca ttggtgaata ccctgtttca acttgtcgta aataagattt ggaaatacca
      541 agcttttttg caaagtcagc tttgaaacca cgagggcgat ctgacaaata actagttaag
      601 ttcattaaca ctcccgttaa gtaaaaatta gattgagttt agatttaact aaatacaaag
      661 tcaagtaaag tttagctttt gcttgtttag gaaattctaa ataaaataat ttaaaaatga
      721 aaagtgaggt taattatgat taaaggtatg actctgccag agattcgcag aaaaaaactt
      781 acagaatggt tcaacacaag agatattcca gaaaaggaaa aaagctacat atcacaatta
      841 aaatcaggaa aatcatcttt tggtgagcgt gcagcaagaa gattagagaa tgaatatgga
      901 atgcctagct tttatttgga ctatgatgaa gaaacagaaa gtgcgattgt gtctgctatg
      961 tcaattcaag aaacacagtc tcatcaatac cccattcatc taattgactt caaagctaag
     1021 gcgggcgaaa caggttttat caatactaac tatcctgaaa ttattcaatc tatctatttc
     1081 tctttagacg gcttgcttga aattgtaggg cgaaaatcaa gcaatggcat tgagatgatc
     1141 accattccaa ctgatagtat gagcccaaca ataaacaaag gggatgttgt ttttattgat
     1201 actacaatca attattacaa taacgagggt gtttatattt ttgttattga tgatgaagtt
     1261 tatataaaaa gactacaaaa aattccaggt ggtatttata gagccctatc ggacaacaag
     1321 acttacgaac cttttgatat aaaaagagaa accttagata ctgctattat ccttgggaaa
     1381 tttattcgag tattgccaat aaatcctaaa gacttgtagg cattagcgta gcggttgagc
     1441 atcggagcta tttgtagcca gcggtt
//
LOCUS       DANKBV010000236         1409 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.237, whole genome shotgun sequence.
ACCESSION   DANKBV010000236 DANKBV010000000
VERSION     DANKBV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.237"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..409)
                     /locus_tag="PWY43_002481"
     CDS             complement(<1..409)
                     /locus_tag="PWY43_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL2137303.1"
                     /translation="MNRITQILNIKHPIVQAPMSWLTDAHLVASVAEAGGLGFLAPHA
                     GQPTNPTSNEEVLDRMRNEIRKVKALTDKPFGVPFILSYDFSLIPLMVDLFIEERVPV
                     VLDNGWLDPEIYAKFKAAGIKIICRLPNPNLADA"
     gene            592..>1409
                     /locus_tag="PWY43_002482"
     CDS             592..>1409
                     /locus_tag="PWY43_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137304.1"
                     /translation="MSIRWENIKPLQGSQNSAFEELVCQLARQEFKSQGKFTRISAPD
                     GGIEAMCELPDGSVCGWQAKYFLSSFSSSQWQQIEDSFKESLKNYPKLTKYYVCVATD
                     RANANVSGKKSFLTKWEEYTQRWKDFAQSQGREIEFEFWGSFELSDLLSKPENAGKKF
                     FWFNESELSDKWFEQYNQQAINNLGVRYTPEINVDLPIAMQLESLARTQIFKKHLSDE
                     FHKLLVEIKSQYKSLYRYEALENHFEPVYELLINKLNLLNINNLETTIDIHLIT"
BASE COUNT          484 a          240 c          275 g          410 t
ORIGIN      
        1 aagcgtccgc caaattgggg ttaggcaaac ggcagatgat tttaatgcca gcagctttga
       61 atttggcgta aatctctggg tcaagccagc cgttatccag caccacaggc acacgctctt
      121 caatgaataa atccaccatt aacggaatca gcgaaaaatc ataagacaaa ataaatggta
      181 cgccaaaagg cttatcggtc aatgccttaa ccttacgaat ctcgttacgc atacggtcaa
      241 gtacttcttc gttggaagtt ggattggtgg gttgccctgc gtgtggggct aaaaaaccaa
      301 gcccaccagc ctcggcaacg cttgctacca gatgagcgtc cgtcagccaa gacattggcg
      361 cttggacgat agggtgcttg atatttaaga tttgggtaat gcggttcatt ggattttcct
      421 gtttgaataa aaagaattgg acgtagttta atggttttgg atttgagagg gaatgcgtga
      481 aaatgggaag gtggttttcg gatttggaaa attatcaatg ctaaaatact tgaaatacac
      541 acgccaatcc tcatatcaac aaaattgaat atttaattat ggtaaaagaa aatgtctatt
      601 agatgggaaa acatcaagcc cttacaaggt tctcaaaata gtgcttttga agagctagta
      661 tgccaattgg cacgccaaga atttaaatct caaggaaaat ttactcgaat ttctgcacct
      721 gatggtggga ttgaagcaat gtgtgaattg ccagatggtt ccgtatgtgg ttggcaagcc
      781 aaatattttt taagctcatt ttcttcatca caatggcaac aaattgaaga ttcctttaaa
      841 gaatctttaa aaaactatcc taaactaacc aaatactatg tttgtgtggc aaccgataga
      901 gcaaatgcca atgtatctgg aaagaaatca tttcttacca aatgggagga gtatacccaa
      961 agatggaaag actttgctca atcacaaggt cgtgaaatag aatttgaatt ttggggaagt
     1021 tttgaattat ctgacttatt atcaaaacct gaaaatgcag gcaaaaaatt cttttggttt
     1081 aacgaaagcg aattatctga caaatggttt gaacaataca atcaacaagc cattaataat
     1141 cttggtgtgc gttatacacc tgaaattaat gttgatttac ctattgcaat gcaattagaa
     1201 agcttggcaa gaacccaaat tttcaaaaaa catctgagtg atgaatttca taaattgtta
     1261 gtagaaatta aatcacaata caaatctctt tatcgttacg aagcgttgga aaatcatttt
     1321 gaacctgtat atgaactttt gattaacaaa cttaacttat taaatataaa taatttagaa
     1381 acaacaattg atattcatct aataactaa
//
LOCUS       DANKBV010000237         1389 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.238, whole genome shotgun sequence.
ACCESSION   DANKBV010000237 DANKBV010000000
VERSION     DANKBV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.238"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..549
                     /locus_tag="PWY43_002483"
     CDS             <1..549
                     /locus_tag="PWY43_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HDL2137305.1"
                     /translation="KDGTANAESLKDQENTVWVQQEGWLKGGSYLAVRRVKMFLETWD
                     RTHLQSQEETFGRHRDSGAGIGHQHEFDKLDLAQKDEKGNAVIPEVSHAHLANKTGLK
                     ILRRSFSYSSGIDDKGQFDSGLLFVSFQQSPEQFIKIQNALGNVDKMNEYITHIGSGL
                     FACFAGVKDENDYLGKALFDLI"
     gene            560..703
                     /locus_tag="PWY43_002484"
     CDS             560..703
                     /locus_tag="PWY43_002484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137306.1"
                     /translation="MKLKALVLIPLLFSSTGLLAKVNVSPLFVQLSDAMAESKKGEFA
                     KSS"
     gene            707..1180
                     /locus_tag="PWY43_002485"
     CDS             707..1180
                     /locus_tag="PWY43_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+/Pb2+ permease"
                     /protein_id="HDL2137307.1"
                     /translation="MTALQHDFEQISQHDSLAGVEVQTALKKAIETPNAENLESLSKK
                     LYAFEKEQNPSGYAEKHQDFVAKMTPLYAELAQTVPTQEIAKIKWAAYQFGKNWVKQE
                     KAVREISLPHYGKFERILGLMRINLNAEKPDMAKISELVSELGAVMADFKQVKVK"
     gene            complement(1216..>1389)
                     /locus_tag="PWY43_002486"
     CDS             complement(1216..>1389)
                     /locus_tag="PWY43_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDL2137308.1"
                     /translation="VSGCGQTPDQRQLLARWAKEFDLYGSVGSDFHYPTGWIELGKSL
                     ELPQECKPIWTLF"
BASE COUNT          446 a          279 c          308 g          356 t
ORIGIN      
        1 aaagacggca ctgccaacgc agaaagttta aaagatcagg aaaatacggt atgggtgcaa
       61 caagagggct ggctcaaagg cggttcttat ttagccgttc gccgtgttaa aatgttcttg
      121 gaaacttggg atcgcaccca tctccaatcc caagaagaaa ccttcggtcg tcaccgtgat
      181 tccggagccg gaatcgggca tcagcacgaa tttgataaat tagatttagc ccaaaaagac
      241 gaaaaaggca atgcagtaat tccggaagtt tcacacgctc atcttgccaa taaaacgggg
      301 ctgaaaatcc tacgccgttc attctcctat tcaagcggta ttgatgataa agggcaattt
      361 gattcagggt tgttgtttgt ctctttccaa caaagtccgg agcaatttat caaaatccag
      421 aacgctctcg gcaatgtgga taaaatgaat gaatacatca ctcatatcgg cagtggttta
      481 tttgcctgtt ttgccggtgt gaaagatgaa aatgactacc ttggcaaagc gttgtttgat
      541 ttaatttagg gaaaaggaaa tgaaattaaa agcattagtg ctaattccgt tactcttttc
      601 gagtacagga ttgcttgcca aagtgaatgt cagcccctta tttgttcagc tttccgatgc
      661 aatggcagag agtaaaaaag gggaatttgc aaaatccagc taaaatttga ccgctttaca
      721 gcacgatttt gagcagatta gccagcacga ttccctcgcc ggagttgaag tacagaccgc
      781 tctgaaaaaa gcgattgaaa cacccaatgc tgaaaactta gagagccttt ctaaaaaact
      841 ctatgcgttt gaaaaagaac aaaaccctag cggttatgct gaaaaacatc aggattttgt
      901 ggcaaaaatg acgccgcttt atgcagaact tgctcaaacc gtgccaactc aagaaatcgc
      961 caaaatcaaa tgggcggctt atcagtttgg taagaattgg gtaaaacagg aaaaagcagt
     1021 acgggaaatc agtcttcccc attatggcaa atttgagcgt attcttggtt taatgcgaat
     1081 taatctgaac gctgaaaagc cggatatggc aaaaatcagt gaactggttt ccgaactagg
     1141 tgcggtaatg gcagacttta agcaagttaa agtgaaatag taaaaaatcg gggcaaatta
     1201 tttgcctcga taacactaaa ataacgtcca aatcggttta cattcttggg gtaattccaa
     1261 acttttaccg agctcaatcc agccagttgg gtagtggaaa tcagaaccga ccgagccata
     1321 taaatcaaac tcttttgccc aacgggcaag taattgccgt tgatcaggtg tttgtccgca
     1381 gcctgatac
//
LOCUS       DANKBV010000238         1360 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.239, whole genome shotgun sequence.
ACCESSION   DANKBV010000238 DANKBV010000000
VERSION     DANKBV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.239"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..264
                     /locus_tag="PWY43_002487"
     CDS             <1..264
                     /locus_tag="PWY43_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137309.1"
                     /translation="DEARGRFAKSLALAISKEQLSADFIKKMTAIITPHKEGTLPLHF
                     YYQSPEGRVLLKGGVEWRISPNEAMFSELKALLGENSVELEFE"
     gene            complement(303..674)
                     /locus_tag="PWY43_002488"
     CDS             complement(303..674)
                     /locus_tag="PWY43_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137310.1"
                     /translation="MKLKYLLATILLAFSTFTVAQSIKTEMMQMNMQANKLMRAESAE
                     EFSEIANDFIIITEKTKGIFPNSVEGDKAAQEDYQAGMQKLIDMVNQAIEKAQAGELQ
                     EAKMLISELEMIKREYHKKYK"
     gene            802..>1360
                     /locus_tag="PWY43_002489"
     CDS             802..>1360
                     /locus_tag="PWY43_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2137311.1"
                     /translation="MTLKYAKPELLSPAGSLKNMRYAFAYGADAIYAGQPRYSLRVRN
                     NEFNHANLKVAIDEAHALGKKFYVVVNIAPHNSKLKTFIKDLKVVVDMKPDALIMSDP
                     GLIMLVRENFPEMDIHLSVQANAVNWATVKFWRQMGLTRVILSRELSLEEIEEIREQV
                     PDMELEIFVHGALCMAYSGRCLLSGY"
BASE COUNT          382 a          262 c          287 g          429 t
ORIGIN      
        1 gatgaagcaa ggggtcgatt tgccaaaagt ttagcgttag cgattagcaa agagcaactt
       61 tctgcagatt ttattaaaaa aatgaccgct atcatcaccc cacataaaga aggcacttta
      121 ccgctgcatt tttattacca aagcccggaa ggtagggtat tgctcaaagg cggagtagag
      181 tggcgaatca gcccgaatga ggcaatgttt agcgaattaa aggcacttct tggtgagaat
      241 tcggttgaac tggaatttga atagaaaggg tgagctttag ctcacccttt taatgtgttg
      301 aattatttgt attttttgtg atattcacgc ttgatcattt caagctcgga aatcagcatt
      361 tttgcttctt gcaattcacc tgcttgagct ttctcaatgg cttgattaac catatcaatt
      421 aatttttgca taccggcttg gtaatcttcc tgagctgctt tatcgccttc tacgctattg
      481 ggaaaaatcc ctttggtttt ctcggtaatt ataatgaaat cattcgcaat ttcgctaaac
      541 tcttctgccg attcggcacg catcaattta ttcgcttgca tattcatttg catcatctcc
      601 gtcttgatgc tttgagcgac ggtaaatgtt gaaaaagcca gtaatattgt tgctaaaagg
      661 tatttaagtt tcatattttt ccccacataa aaatcggatt gtaatggatt agcttgccat
      721 cacgcaatat ttcttagaaa atataccgct tacgcttctc acgtcgagaa gtcctgactt
      781 gttatcagaa aagagaataa tatgacttta aaatatgcca aacctgagct actttcgcct
      841 gcaggttctt taaaaaatat gcgttacgct tttgcttatg gtgccgatgc gatttatgca
      901 ggacagcccc gttatagctt gcgtgtgcgt aataatgaat ttaaccacgc caatttaaaa
      961 gttgcgattg atgaagccca cgccttagga aaaaaattct atgtggtggt aaacattgcg
     1021 ccacacaact caaaattaaa aaccttcatc aaagatctaa aagtggtagt ggatatgaag
     1081 ccggacgctt tgattatgtc tgatccgggc ttaattatgt tggtgcgtga aaatttccct
     1141 gaaatggata ttcatctttc ggtgcaagcc aatgcggtaa actgggcaac ggtgaaattc
     1201 tggcgtcaaa tggggctaac ccgtgtgatt ttatcccgtg aattatcact cgaagagatc
     1261 gaagaaattc gtgaacaagt gccggatatg gagcttgaga tcttcgttca cggtgctttg
     1321 tgtatggcat attcaggtcg ctgcttgctt tcaggctata
//
LOCUS       DANKBV010000239         1349 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.240, whole genome shotgun sequence.
ACCESSION   DANKBV010000239 DANKBV010000000
VERSION     DANKBV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.240"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            45..1049
                     /gene="gap"
                     /locus_tag="PWY43_002490"
     CDS             45..1049
                     /gene="gap"
                     /locus_tag="PWY43_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2137312.1"
                     /translation="MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVDYMAYM
                     LKYDSTHGRFDGTVEVKDGQLVVNGKAIRVTAERDPANLKWNEIGVDIAVEATGLFLD
                     DETARKHITAGAKKVVLTGPSKDATPMFVNGVNFDTYAGQDIVSNASCTTNCLAPLAK
                     VIHEKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPALNGKLTGMAFRVPTTNVSVVDLTVNLEKPATYAEICAEIKRASENEMKGVLGYTE
                     DAVVSTDFNGATETSVFDAAAGIALTDTFVKLVSWYDNEVGYSNKVLDLVAHVYNYKG
                     "
BASE COUNT          447 a          232 c          266 g          404 t
ORIGIN      
        1 taaagaacaa attttgttta actttaatat aggtagaaaa atctatggca attaaaattg
       61 gtattaacgg ctttggccgt atcggtcgta tcgttttccg tgcagcacaa caccgtgatg
      121 acatcgaagt ggtaggtatc aacgacttaa tcgatgttga ttatatggct tatatgctga
      181 aatatgattc aactcacggt cgttttgacg gtactgttga agttaaagac ggtcaattag
      241 tagttaacgg taaagcgatc cgcgtaacag ctgagcgtga cccagctaac ttaaaatgga
      301 atgagatcgg tgttgatatc gcagttgaag caacaggttt attcttagat gatgaaactg
      361 ctcgcaaaca catcaccgca ggtgcgaaga aagttgtttt aaccggtccg tctaaagatg
      421 caacacctat gttcgtaaac ggtgtaaact tcgatactta tgcaggtcaa gacatcgtat
      481 ccaacgcttc ttgtacaacg aactgtttag caccattagc taaagttatt cacgaaaaat
      541 tcggcattaa agaaggttta atgacaactg ttcacgcaac tactgcaacg caaaaaacag
      601 tagatggtcc atcagcgaaa gactggcgtg gtggtcgtgg cgcatcacaa aatatcattc
      661 cttcatcaac aggtgcagcg aaagcagtag gtaaagtatt acctgcatta aacggcaaat
      721 taaccggtat ggctttccgt gttccaacca ctaacgtttc tgttgttgat ttaactgtaa
      781 acttagaaaa accagcaact tatgcagaaa tctgtgctga aatcaaacgt gcttcagaaa
      841 acgaaatgaa aggcgtatta ggctacacag aagatgcagt tgtttcaaca gacttcaatg
      901 gtgcaactga aacttcagta tttgatgcag cagcaggtat cgcattaact gatactttcg
      961 ttaaattagt atcttggtac gataacgaag ttggctactc aaacaaagta ttagacttag
     1021 ttgctcacgt atataactac aaaggttaat tgctaagtta tattttaaat atagagccaa
     1081 attctgaacg gaatttggct cttttgctgc gaacaataaa ttgaatatag tcgcgttttc
     1141 ccataaaatc aatttcctac aaacaggaac taagcacagt aaggcagatt ttcttgctct
     1201 ttgcagttta ttttgtgtaa ataaatatta ttagagacta caaacgatta ataaatatca
     1261 agtttcctat gaaaaatgat gtttttatta atgctatatt gctttttgag agatgattta
     1321 aattattgat tataagtatg gtaagatta
//
LOCUS       DANKBV010000240         1307 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.241, whole genome shotgun sequence.
ACCESSION   DANKBV010000240 DANKBV010000000
VERSION     DANKBV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.241"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(198..491)
                     /locus_tag="PWY43_002491"
     CDS             complement(198..491)
                     /locus_tag="PWY43_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137313.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYKYLKETGQIVV"
     gene            635..1024
                     /locus_tag="PWY43_002492"
     CDS             635..1024
                     /locus_tag="PWY43_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2137314.1"
                     /translation="MADPIEWSIITTDCFDEWFGQQDESTQERVLACLIYLRKFGYSL
                     GRPYADTVYQSKFPNMKELRIQNKGKPIRAFYAFDPLRQAIVLCAGEKGKDKRFYEKM
                     IALADAEFTSYLNNLEKENENVRSNAQ"
     gene            996..1295
                     /locus_tag="PWY43_002493"
     CDS             996..1295
                     /locus_tag="PWY43_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2137315.1"
                     /translation="MKTLDQMLNELSPERRQKVEDMAQEMIREIQLSKLREELNLSQQ
                     QLATALGISQPAIAKLEQKDNDPRLSTLKRYIEAMGGKLKLAVELPTGDERVIRI"
BASE COUNT          421 a          235 c          239 g          412 t
ORIGIN      
        1 aaacgttccg aaaaaacatt gaatttcatt aaaaaattcc tttatttatc aaataaatgc
       61 gatttgtttc ggaacgtttt ttaatgctta ttttgcaaca tacggattat caatggaaag
      121 cgttgctgta gacattgtgc ctcctagtac aaaaaaaccg cttggtttcc ctaagcggtt
      181 ctaatctata agaactatta caccacaatc tgccccgtct cttttaagta cttatagata
      241 cgccctaaca taatctgcgt tggcgttttg ccaatatcct cttttgttag cggtgcatca
      301 gtaatcgctt tggctgattg agcatcaata tatttgtact cacttgtgat gattacaaag
      361 tttgttacct cgccatcggt atcatcacct gtgccaacaa catacttagc attgagtgag
      421 ccgtcatctt gagttgagta acttgcgata gttgagtaca ttgggtttaa gattttgtta
      481 aaagttgtca taatttttcc ttttgtggat tgttgataaa aaaagaccgc ttgtagtcat
      541 acaaacggct tagtaggaag agtaaattgc tttacataat ataacctttt atttatactt
      601 catttataaa tagtaagtta taaaaggttc aaaaatggca gaccctatag aatggtctat
      661 tatcacgact gactgctttg atgaatggtt cggtcaacaa gatgaaagta ctcaagaacg
      721 tgtcttggct tgtttaatct atctgcggaa attcggttat agtctaggca gaccttatgc
      781 tgatacagtt tatcagtcta aattccctaa tatgaaagag ttacgcattc aaaacaaagg
      841 taagccaatt cgagcatttt atgcttttga ccctttacgc caagcgatag tactttgtgc
      901 tggcgagaaa ggtaaagaca aaagattcta tgaaaaaatg atcgctcttg ctgatgcgga
      961 atttacctcc tatttaaata atttagagaa agaaaatgaa aacgttagat caaatgctca
     1021 atgaactctc tcctgaacgc cgccaaaaag tggaagatat ggctcaagaa atgattagag
     1081 agatacaact ttcaaaacta agagaagagc taaatctctc tcaacaacaa ttagctacag
     1141 ctctcggtat ttcacagcct gctattgcta aacttgaaca gaaagataac gatccaagat
     1201 tatccacgct caaacgctat attgaagcaa tgggcggtaa gttaaaactt gccgttgaat
     1261 tgcctacagg cgatgagcgt gtaattagga tttaattact ttgttaa
//
LOCUS       DANKBV010000241         1295 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.242, whole genome shotgun sequence.
ACCESSION   DANKBV010000241 DANKBV010000000
VERSION     DANKBV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.242"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..471
                     /gene="nrdG"
                     /locus_tag="PWY43_002494"
     CDS             <1..471
                     /gene="nrdG"
                     /locus_tag="PWY43_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL2137316.1"
                     /translation="ENLRFSSEQIVWQEVPNETSLAFLITGCPLGCKGCHSADSWKAC
                     LGKELSSEYLSERLKRYQGLISCVLFMGGEWEAEKLLPLLQLAKKQGLNTCLYSGLEQ
                     HEIAETLLHELTYLKTGRWIAERGGLESLTTNQRFTDLRNNQNLNHLFIRGASI"
     gene            468..>1295
                     /locus_tag="PWY43_002495"
     CDS             468..>1295
                     /locus_tag="PWY43_002495"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611280.1"
                     /note="Catalyzes the reduction of nucleoside
                     5'-triphosphates to 2'-deoxynucleoside 5'-triphosphates;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2137317.1"
                     /translation="MIRLQPTQLDGKLHFINQYIQAKNAADGSKMDANANVTQKNIAT
                     MEQELMKDFFVQINRAKVSEKIAKIFGKETACEYLRQIEAHEIYVHDETSLKPYCVSV
                     TMYPFLRDGLTKLGGESKAPKHLASFCGSFINFVFAVSSQFAGAVATVEFLTYFDYFA
                     RKDYGDNYLQTHRSDIENHLQQVVYSINQPAAARGYQSVFWNISVYDKFYFEAMFSRF
                     VFPDFEKPNWETTSKLQLFFMQWFNKERSKAILTFPVVTAAMLTEKGKCKDDEFANQM
                     "
BASE COUNT          403 a          275 c          267 g          350 t
ORIGIN      
        1 gaaaatctca gattcagctc ggaacaaatt gtttggcaag aagtgccgaa tgagacctca
       61 cttgcttttc tgattacagg ctgcccgctt ggctgtaaag gttgccatag tgcggatagc
      121 tggaaagcct gtcttgggaa agagcttagc agtgaatatc tctctgaacg cctaaaacgc
      181 taccaaggct taattagctg cgtattgttt atgggcggag aatgggaagc agaaaaatta
      241 ctgccgctgt tgcaactggc aaaaaaacag gggctaaaca cctgtcttta tagtggtttg
      301 gagcagcacg aaatcgccga gactttatta cacgagctaa cctacttaaa aaccgggcgt
      361 tggattgccg aacgaggagg attggaaagt ttaaccacca accaacgctt tactgattta
      421 cgcaataacc aaaatcttaa ccatcttttt attcgaggag cttcaatatg attcgcttac
      481 aaccgacaca attagacgga aaactacatt tcattaacca atatattcaa gccaaaaatg
      541 ccgcagacgg ttcaaaaatg gacgccaacg ctaacgtcac gcaaaaaaac atagcgacaa
      601 tggaacagga gctaatgaaa gatttttttg tgcaaatcaa ccgagcaaaa gtgagtgaaa
      661 aaattgcaaa aatttttgga aaagaaaccg cttgtgaata tttacgtcaa atcgaagcac
      721 acgaaattta cgtacacgat gaaaccagcc taaaacccta ctgcgtttca gtcactatgt
      781 accctttctt gcgtgatggt ttgactaaac tcggcggcga atccaaagcc ccgaaacatt
      841 tagcctcatt ctgcggttct tttatcaatt ttgtctttgc agtcagttct cagtttgccg
      901 gtgcagtcgc cacggttgaa tttctgacct attttgacta ttttgcccgc aaagattacg
      961 gcgataatta cttacagact catcgttcag atattgaaaa ccacctgcag caggttgtgt
     1021 atagcatcaa ccaaccggca gccgctcgtg gttatcaaag cgtcttttgg aatatttcag
     1081 tgtatgacaa attctatttt gaagcgatgt ttagccgttt tgtttttcct gattttgaga
     1141 aaccaaattg ggaaaccact tcaaaattac aacttttctt tatgcaatgg ttcaataaag
     1201 agcgaagcaa agcgattctc actttccctg ttgtgaccgc agcaatgctt accgaaaagg
     1261 gcaaatgtaa agatgacgag tttgccaatc aaatg
//
LOCUS       DANKBV010000242         1274 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.243, whole genome shotgun sequence.
ACCESSION   DANKBV010000242 DANKBV010000000
VERSION     DANKBV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.243"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..320
                     /locus_tag="PWY43_002496"
     CDS             <1..320
                     /locus_tag="PWY43_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137318.1"
                     /translation="YLEEYNDLGTIRYKFQEENLYGFLRDGATLVANGIVNEPSVDCF
                     SQEIAQFTGCHIFSSLYIAFNTQRSFKSHWDSRDIFAVQMQGKKRWIIHTPTFKKTIV
                     YAS"
     gene            310..960
                     /locus_tag="PWY43_002497"
     CDS             310..960
                     /locus_tag="PWY43_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HDL2137319.1"
                     /translation="MHRSKDMPEYAPNLDDVYMDIVLEAGDVLYLPRGWWHDPIPVGE
                     ETVHLAVGIFPAYTHNYLTWVSQNMVEKEIARASLSHYESDKELIAQLAEQTAEYIKD
                     KENYHKFIENFYDQKRVEKPLNLETLGNYQYDSISENQKISFKAKNHYFGYENKIISN
                     GYGISLDEEFGDVIKFLKQGQEVSLNDILEKISEDKRDKVSQLIWQLSYIGVLKLS"
BASE COUNT          435 a          148 c          235 g          456 t
ORIGIN      
        1 attatttaga agaatataat gatctaggta ctattcgtta taaatttcaa gaagaaaatt
       61 tatatggttt tttaagagat ggggcaacat tagtcgcaaa tggtattgta aatgaaccaa
      121 gtgttgattg cttctctcaa gagatagctc aatttacagg ctgtcatatt ttttcaagtt
      181 tatatattgc gtttaatact caaagatctt ttaaaagcca ttgggatagt cgtgatattt
      241 ttgcagttca aatgcagggt aaaaagcgtt ggattattca cactccaaca tttaaaaaaa
      301 ccattgttta tgcatcgtag taaagatatg cctgaatatg ctcctaatct tgatgatgta
      361 tatatggata ttgtattaga agctggagat gttctttatt tacctcgtgg ctggtggcac
      421 gatcctattc ctgttggaga agaaactgtg catttagctg taggcatttt tcctgcttat
      481 actcataact atctgacttg ggtatctcaa aatatggttg agaaagaaat tgcccgagct
      541 tctttatcgc attatgagtc agataaagag ttgatagcac agttggcaga acaaacagca
      601 gaatatatta aagataaaga gaattatcat aaatttatag agaattttta tgatcagaag
      661 cgggttgaaa agccacttaa tttggaaact ttaggaaatt atcaatatga ttctatttcg
      721 gaaaatcaaa aaatttcatt taaagccaaa aatcactact ttggttatga aaataagata
      781 atttcaaatg gatatggtat ttctcttgat gaagagtttg gagatgttat taaatttctt
      841 aaacagggtc aggaagtttc attaaatgat attttagaaa aaatatcaga agataaacga
      901 gataaagttt ctcagcttat ttggcagtta tcttatattg gtgtgctaaa attgagttaa
      961 ataagtaagg ttttactata gttattttgt tagcctctag atagaggcta atattaaaat
     1021 aatttattag gttgagctat tttttctcta agtttaatta taaatatagt aatcttataa
     1081 tttatcctaa atacttcaga agtatagatt ataagttatg aatgtaggtg gtatcgatgt
     1141 tgctattgat tcttaggacg ttattacctt taataatgtt atctgcttgt gctgactatc
     1201 aagaatatga taaaaaggat agtaaatgtt attattcttc ctatggaagt ggagaggcaa
     1261 aagattctgt tagg
//
LOCUS       DANKBV010000243         1268 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.244, whole genome shotgun sequence.
ACCESSION   DANKBV010000243 DANKBV010000000
VERSION     DANKBV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.244"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1081
                     /gene="malK"
                     /locus_tag="PWY43_002498"
     CDS             <1..1081
                     /gene="malK"
                     /locus_tag="PWY43_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDL2137320.1"
                     /translation="GDVHISKDINLEINEGEFVVFVGPSGCGKSTLLRMIAGLEDITT
                     GELYIGGKLMNEVEPSKRGIGMVFQSYALYPHLDVAENMSFGLKLAGVSKAERDQRVN
                     QVAEILQLAHLLDRKPKALSGGQRQRVAIGRTLVSQPEVFLLDEPLSNLDAALRVQMR
                     VEISKLHKKLNRTMIYVTHDQVEAMTLADKIVVLNAGGIAQVGKPLELYHYPANRFVA
                     GFIGSPKMNFLPVKVIAVEPERVKIELPDANHHNFWIPVSGEGVNVGDNLSLGIRPEH
                     LVPSDQTQVTLHGIVQVVELLGNETQIHLEIPEIKQPTLIYRQNDVVLVNEGDDLNIG
                     ILPERCHLFKEDGTACKRLFVEKGV"
BASE COUNT          390 a          236 c          280 g          362 t
ORIGIN      
        1 tggtgatgtt catatctcta aggatattaa tttagaaatt aacgaaggag aattcgttgt
       61 tttcgttgga ccgtcaggtt gtggcaaatc tacgctatta cgaatgatag caggtttgga
      121 agatattacg acaggagaac tctatatcgg tggaaaactg atgaatgaag tagagccttc
      181 aaaacgtggt atcggtatgg tgttccaatc ctatgctctc tatccacatt tagacgttgc
      241 cgaaaatatg tcttttggct taaagctggc aggtgtaagt aaagcagagc gggatcaacg
      301 agttaaccaa gttgccgaaa tcttacagct tgcccattta ttagatcgaa aacctaaagc
      361 actttccggc ggacaacgcc aacgggtggc tattggtaga acactggttt cccaaccgga
      421 agtattttta ctcgatgaac cactttccaa ccttgatgcc gcattgcgtg tacaaatgcg
      481 ggtagaaatt tccaaattac ataaaaaact aaaccgcaca atgatttatg ttacgcacga
      541 tcaagttgaa gcaatgacat tagcggacaa aatcgtggta ttaaatgccg gcggtattgc
      601 tcaagtaggt aaaccgttag aactttatca ttacccggca aaccgctttg tagcagggtt
      661 tatcggctcg cctaaaatga actttttacc ggtaaaagtg attgcggtag agccggaacg
      721 ggtaaaaatt gagttacctg atgcgaatca tcataatttt tggattccgg tttccggcga
      781 aggggtgaat gtgggagata acttatcttt aggtatccgc ccggaacatt tagttccttc
      841 ggatcaaacc caagtgacct tacacggtat tgtgcaagtt gtggaattgc tcggaaatga
      901 aacacaaatc caccttgaaa tccctgaaat caaacaacca accttaattt atcgtcaaaa
      961 cgatgtggta ttagttaatg aaggcgacga tctgaatatc ggtatattgc cggaacgctg
     1021 ccatttattt aaggaagacg gtacagcttg caaacgtttg tttgttgaaa aaggggtata
     1081 acaatttcag tttattactg attttagagg gtattttcaa tcttctaatc aattaataat
     1141 taacctaagt aaaatcaaat cttaagagag gttctattat gaaaaaaaca gtcctagcag
     1201 ttgcaatcag tggtgcaatg ttcgcagcca gtgcatcagc ggttgatttc cacggatatg
     1261 cacgttca
//
LOCUS       DANKBV010000244         1267 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.245, whole genome shotgun sequence.
ACCESSION   DANKBV010000244 DANKBV010000000
VERSION     DANKBV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.245"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(1..460)
                     /locus_tag="PWY43_002499"
                     /pseudo
     CDS             complement(1..460)
                     /locus_tag="PWY43_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251678.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(692..1114)
                     /locus_tag="PWY43_002500"
     CDS             complement(692..1114)
                     /locus_tag="PWY43_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005579070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HDL2137321.1"
                     /translation="MRALIHTFRSTTLDITILRLSVCLIFFTFGMTKWFDFEVEILKP
                     MISPTWLNFLYEWLGYHGTSYFLGVVEGIAYIALAVGYWKPRWGIIGSMTVLLVSSVT
                     LSLLLQLGFNSFIFKDILLIGAGLVLLKYDLNRLYPHE"
BASE COUNT          424 a          233 c          226 g          384 t
ORIGIN      
        1 ttcttgctca ttgagtggta cgcctgcgtt taaaccactc tttaatttcg ctttcagtgc
       61 ctttgcattc ataaattaat aacgtggtat ttaaaatgaa ttggcgttta tcttcaggca
      121 aactggaaaa atagcaaggg atattatttt gatctaaaat ggcaaattta ttcgtaacaa
      181 atcgcccgat actagtaatg cgttgctgac cgtctaacac ttcaaattta tcatcatctg
      241 tttgattaaa ataaatcaaa ccaagtggat agcctttcaa aatagagtga ataaccgcca
      301 catctttttt accgtccgca taaatatagt tacgttgata ttcaggctga atagttaatt
      361 taccgtttaa accaaataag cctttgcctt caagttcgtt atattgaaaa ccttcgcaca
      421 gtttttcaat ggtgatttcg gtatttaatt tcgtttgcat tttgtctcca agtcgggcat
      481 aaatgcccga aaatttatat tatcggtaca atggttgaat atatcggaaa attgtcgggc
      541 atagatgccc gacctacttt ttatggcgga taaaaatacg ttgataagtg gattgtacaa
      601 tatgaccatc ttttcattta attcaaggag tgagttctgt attactcacg gcttagtcca
      661 acttttgggg cagatcaaaa aacaacgtga gctattcgtg ggggtaaagg cggtttagat
      721 cgtattttaa taatactaat cctgcgccga taagtagaat atccttgaaa ataaagctgt
      781 taaaaccaag ctgtagcaac aggctaagtg taacagatga aaccagtaat actgtcattg
      841 aaccgataat tccccaccta ggtttccaat agccaacggc aagtgctata taagcaatcc
      901 cttcaaccac acctaagaaa taacttgtgc cgtgatagcc tagccactca tagagaaaat
      961 tcaaccaagt cggactgatc atcggcttaa ggatttccac ctcaaaatca aaccattttg
     1021 tcataccgaa agtaaagaag attagacata cgctaagacg taaaatcgtg atgtctaaag
     1081 tcgtgctacg aaaagtgtgg ataagtgctc tcatcgtcat acctaagtta aaaaatgaag
     1141 tatattagac atagatcaag cgtgtttctg acaagtattt gcaatttaaa atgaaaatcg
     1201 gtattttcag caccattaat caaagctatc acagagaata aaatgaacag tacagaattg
     1261 atgaaat
//
LOCUS       DANKBV010000245         1265 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.246, whole genome shotgun sequence.
ACCESSION   DANKBV010000245 DANKBV010000000
VERSION     DANKBV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.246"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..725
                     /gene="zipA"
                     /locus_tag="PWY43_002501"
     CDS             <1..725
                     /gene="zipA"
                     /locus_tag="PWY43_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDL2137322.1"
                     /translation="QPQNEDYQAMSVPEENPQQIQQEVESSLQNIKISLPGGNPQQQP
                     EQIQPIQSVAEPTVSTYDQKISNQQHIQYNTPDFANPMVNTVVEETPQAVAIEPQETQ
                     SAEEENPFVTLYLYAPQNEAFRGDFVVHYLEEVGLRFGEHQIFHRHQHIDDSLSPIIF
                     SAANLMNPGTFDLNNISNFATAGLVLFMQLPTEGNDVANMKLLISNAEHLAASLGGFL
                     YDDNQQPFGEESRQRYMQRVMH"
     gene            790..>1265
                     /gene="ligA"
                     /locus_tag="PWY43_002502"
     CDS             790..>1265
                     /gene="ligA"
                     /locus_tag="PWY43_002502"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252997.1"
                     /note="this protein catalyzes the formation of
                     phosphodiester linkages between 5'-phosphoryl and
                     3'-hydroxyl groups in double-stranded DNA using NAD as a
                     coenzyme and as the energy source for the reaction;
                     essential for DNA replication and repair of damaged DNA;
                     similar to ligase LigB; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2137323.1"
                     /translation="MSLFNQTSLFEENATPELVEKIETIRQTLRQYEYEYHVLDNPSV
                     PDAEYDRLMNELKNLEWQHPELISADSPTQRVGAKPLAGFAQVTHEIPMLSLDNAFSD
                     EDLDGFLRRMESYITASPSQLEFCCEPKLDGLAVSILYENGILTRAATRGDGTTGE"
BASE COUNT          401 a          288 c          256 g          320 t
ORIGIN      
        1 tacagccaca aaatgaggat tatcaagcaa tgtctgtgcc ggaagaaaat ccgcaacaga
       61 tacaacagga agttgaaagc tcgctgcaaa atatcaaaat cagtttgccg ggcggaaatc
      121 ctcagcaaca gcctgagcaa attcagccga tacaatcggt agcagaacca acagttagca
      181 cttacgatca gaaaatttct aatcagcaac atatccaata caatacgccg gattttgcaa
      241 acccaatggt gaacacagtg gtggaagaaa cacctcaagc agtagctatc gaacctcaag
      301 aaacccaatc ggcagaggaa gaaaatccat ttgtaaccct ctatctttac gcaccacaaa
      361 atgaagcttt ccgcggtgat tttgtagtac attatttaga agaagttggc ttacgttttg
      421 gcgaacacca aattttccac cgccatcaac atattgatga ttcattaagt ccgattattt
      481 tcagtgcagc aaatttaatg aacccaggaa cctttgattt aaataatatc tcaaactttg
      541 cgacagctgg tttagtgcta tttatgcaac ttccaaccga aggcaatgat gtggctaata
      601 tgaagttgtt aatcagtaac gctgaacact tagcagcatc gttaggggga ttcttatatg
      661 acgataatca acagcctttt ggcgaagaat cccgccaacg ttatatgcaa cgtgtgatgc
      721 actaattgcg aaaaaattaa taaatccgac cgcttgtaca agcggtcttt tttgtagaga
      781 attttaccaa tgtctttatt taaccaaacc tctttatttg aagagaatgc cacgcccgag
      841 ttagtcgaga aaatcgaaac tattcgccaa acattgcgtc aatacgagta tgaatatcac
      901 gttttagata acccaagcgt acccgatgcc gaatacgacc gtttaatgaa cgagctgaaa
      961 aatctggaat ggcagcaccc ggaactgatt agtgccgact cgccgactca acgggttggt
     1021 gccaaaccgc tggcaggctt tgctcaagtc acccacgaaa tccctatgct ttcgttggat
     1081 aatgccttct ccgatgaaga cttagacggc tttttacgcc gtatggaaag ctatatcacc
     1141 gcctctcctt cgcaactgga attttgttgc gaacctaaat tagacggctt ggcagtgagt
     1201 attttatacg aaaacggtat acttacccga gctgcaaccc gtggcgatgg cacaaccggc
     1261 gaaga
//
LOCUS       DANKBV010000246         1244 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.247, whole genome shotgun sequence.
ACCESSION   DANKBV010000246 DANKBV010000000
VERSION     DANKBV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.247"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..356
                     /locus_tag="PWY43_002503"
     CDS             <1..356
                     /locus_tag="PWY43_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialyltransferase"
                     /protein_id="HDL2137324.1"
                     /translation="KVFIKLHPKERIETIDVYKELSKDKQGLIIMENISFPAEDFISQ
                     LKPRKVLSIASTSLVYTTLISKDIKAISIYPLFRKEVLKKIEYKEEYFKDIESHYSLL
                     SKFDGIRILNNTNEI"
     gene            346..>1244
                     /locus_tag="PWY43_002504"
     CDS             346..>1244
                     /locus_tag="PWY43_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137325.1"
                     /translation="MKYSNYKILVVSTYDSFLKVGINLARRINHSSIEVAIQYVKKNQ
                     LSIRQLVESGANNYRVFDYKILNNSDFSKYDIVIAALGNIASQKLITRLYSLNKRPLI
                     ITCFSGSIFGNAESISSRINSDILLVNNESDRNIAQSIASEYGLETTILNYGLINLDL
                     SFQRNKKSGKNIFFIDQVKIPQLKTEREYVLEKLLELARSKPDYSVFLKTRLYGNEIT
                     VHKDQYPYAHLLRNKKNIPTNFSLYNDSIEVAFQEMDICISFSSTSVLEAIYYGIPTY
                     IIKDLGIRESLYNPPFLQSGLLT"
BASE COUNT          464 a          151 c          183 g          446 t
ORIGIN      
        1 caaaggtttt tataaagtta caccctaaag aaaggattga aactattgat gtatataaag
       61 aactatctaa agataagcaa ggtctgatta ttatggaaaa tatttctttt cctgcggagg
      121 attttatatc tcaattaaaa ccaagaaaag ttctatctat tgcctctaca tctcttgttt
      181 atactacttt aatatcaaaa gatattaagg caatttctat atatcctcta tttagaaaag
      241 aagttctaaa gaaaatagag tataaagaag aatattttaa agatatagag agtcattatt
      301 cattactaag taagtttgat ggtattagaa tattaaataa tacaaatgaa atatagtaat
      361 tataaaatat tagtagtttc aacctatgat tcttttttaa aagtaggaat taatcttgcc
      421 agaaggatta atcatagtag tattgaagtt gcaatacaat atgtaaaaaa gaatcaactt
      481 tctattcgtc aattagttga aagtggagca aataactatc gtgtctttga ttataaaata
      541 ttgaataatt cggatttttc aaagtatgat atagttattg cagcactagg taatatagcc
      601 tcgcagaaac taattacacg tttgtattcc ttaaataaaa gacctctgat tattacctgt
      661 ttctcaggga gtatttttgg taatgcagaa agtatatcat ctcgtattaa ttcagatatt
      721 ttactcgtaa ataatgagag tgatagaaat attgctcaat ctatagcatc tgagtacggt
      781 ttggaaacta ctattttaaa ttatgggcta attaatcttg atttaagttt tcaacgaaat
      841 aagaaatctg gtaaaaatat tttttttatt gatcaggtaa aaattccaca gctaaaaact
      901 gaaagagaat atgtattaga aaaacttcta gaactagcta gaagtaaacc tgattatagt
      961 gttttcttaa aaactcgttt atatgggaat gaaataactg ttcataaaga tcagtaccct
     1021 tacgcacatt tactaagaaa taaaaaaaat attcctacaa attttagttt atataatgat
     1081 tccattgaag ttgcttttca ggaaatggat atttgtatat ctttctcttc aacttctgtt
     1141 ttagaagcaa tctattatgg tatccctact tatattatta aagatttagg tattagagaa
     1201 agcttgtata atcctccttt tttgcagagt ggtttgctta caga
//
LOCUS       DANKBV010000247         1233 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.248, whole genome shotgun sequence.
ACCESSION   DANKBV010000247 DANKBV010000000
VERSION     DANKBV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.248"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(1..1190)
                     /locus_tag="PWY43_002505"
                     /pseudo
     CDS             complement(1..1190)
                     /locus_tag="PWY43_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252774.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
BASE COUNT          390 a          205 c          188 g          450 t
ORIGIN      
        1 tctaatgtga ataatttatc taacatattt tctatttgtt ctttattcat gttggtttga
       61 gataggattc tacgccatgc aattttgatt tttgctttaa tttcatctcg tgtttgccag
      121 tcagttacca tattggtttt tagaatattg agtaacttat tggctatttg ttctaggtct
      181 gcatcaggaa gagctagata aggtattaat aattccttaa tattattaat agagctattg
      241 tttgtaagag tagggatact accttcgtta tttgcaagag ttggagactc ccaatgattt
      301 ggcgttgaac tatcaataat tttttggttt ttgttatatg gctgaaattc agaaagtaga
      361 ttgcaatagt cagcagcccc cattaaatca ggaaataaat tggcgtgatg aatattcatt
      421 tgtcgcaaag acaaaatgca atcactttga atgtctggtg tgtttaaaat ataaattttt
      481 cagagatatt ttgctatttc ttgttcgaat gactcatcag agagaaattc actttcgttc
      541 tgctccgcaa tagaatctac aattaaagat tctatcgatt ttcctgcttc tgctttaata
      601 aataaacctg cttgattgac caatctccca tacatatcca ctttgggatc aacaaaggta
      661 acaaagttag aaagtaactt cttatttgca atataaacaa ctcggtattc atttggcttt
      721 tcagctttaa catctttgcg tgaaaaagca aaaaataatg agacataagg agaatatgtc
      781 caatccaata aaggtgtatt caatccataa tgttgcccaa ttgcccaaat ttcatcatca
      841 tcttcattaa gcaataaagt ataatcttta atccgacctc gaatggcttt tttaaagtta
      901 ttaagctgat tttccatcaa ttctttagta aacaagccat tatcactatt tctggcaata
      961 ttgggcatta gtccccaatc atatcggcgt tgccctctaa aaatgagttc agaaatattt
     1021 tcttggaaaa aatttgtaga aagtaaatca ataaactttt tccagccatt tactcttaat
     1081 aaagggacac gactatttat ctcatcgttt ttagagacaa aaataggttc gccactttgc
     1141 cattctttga tttcatcaaa gtaactagaa gataattctt cgttattcat tgaataactc
     1201 tccatttaat aatttaggta gtaactcatc tct
//
LOCUS       DANKBV010000248         1223 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.249, whole genome shotgun sequence.
ACCESSION   DANKBV010000248 DANKBV010000000
VERSION     DANKBV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.249"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            21..1163
                     /gene="tuf"
                     /locus_tag="PWY43_002506"
     CDS             21..1163
                     /gene="tuf"
                     /locus_tag="PWY43_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2137326.1"
                     /translation="MGTIGHVDHGKTTLTAAITTVLAKHFGGAARAFDQIDNAPEEKA
                     RGITINTSHVEYDTATRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQT
                     REHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGS
                     ALQALNGVAEWEEKILELANHLDTYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRV
                     ERGIIRTGDEVEIVGIKDTAKTTVTGVEMFRKLLDEGRAGENVGALLRGTKREEIERG
                     QVLAKPGSITPHTDFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGV
                     EMVMPGDNIKMTVSLIHPIAMDEGLRFAIREGGRTVGAGVVAKIIK"
BASE COUNT          391 a          250 c          288 g          294 t
ORIGIN      
        1 gtacaaaacc gcacgttaat gtgggtacaa tcggccacgt tgaccatggt aaaacaactt
       61 taacagcagc aatcactacc gtattagcaa aacacttcgg tggtgcagct cgtgcatttg
      121 accaaatcga taacgcgcca gaagaaaaag cgcgtggtat cactatcaac acttcacacg
      181 ttgagtacga tacagcaact cgtcactacg cacacgttga ctgcccggga cacgcggact
      241 atgttaaaaa catgattacc ggtgcggcac aaatggacgg tgctatctta gtagtagcgg
      301 caacagacgg tccaatgcca caaactcgtg agcacatctt attaggtcgc caagtaggtg
      361 taccatacat catcgtattc ttaaacaaat gcgatatggt agatgatgaa gagttattag
      421 aattagttga gatggaagtg cgtgaacttc tttcacaata cgacttcccg ggtgatgaca
      481 ctccaatcgt acgtggttca gcgttacaag cgttaaacgg cgttgctgaa tgggaagaga
      541 aaatccttga attagcaaac cacttagaca catacattcc agagcctgag cgtgcgattg
      601 ataaaccatt cttattacca atcgaagacg tattctcaat ttcaggtcgt ggtacagtag
      661 taacaggtcg tgttgagcgt ggtatcatcc gcactggtga tgaagttgaa atcgtaggta
      721 tcaaagatac agcgaaaaca accgtaacag gtgttgagat gttccgtaaa ttattagacg
      781 aaggtcgtgc gggtgagaac gtaggtgcat tattacgtgg tactaaacgt gaagaaatcg
      841 aacgtggtca agtattagcg aaaccgggtt caatcactcc acacacagac ttcgaatcag
      901 aagtttacgt attatcaaaa gaagaaggtg gtcgtcacac tccattcttc aaaggttacc
      961 gtccacagtt ctacttccgt acaacggacg taacaggtac aatcgaatta ccggaaggtg
     1021 tagagatggt aatgcctggc gacaacatca aaatgactgt tagcctaatt cacccaatcg
     1081 caatggacga aggtttacgt tttgcgattc gtgaaggtgg tcgtacagta ggtgcgggcg
     1141 ttgtagcgaa aatcatcaaa taattaagat tgtttgataa agcgaaaagc taaataaaaa
     1201 ctagaaagag gcatatcgaa agg
//
LOCUS       DANKBV010000249         1205 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.250, whole genome shotgun sequence.
ACCESSION   DANKBV010000249 DANKBV010000000
VERSION     DANKBV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.250"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            57..>1205
                     /locus_tag="PWY43_002507"
     CDS             57..>1205
                     /locus_tag="PWY43_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL2137327.1"
                     /translation="MSNNNAKNSNLSNAKKAKSDEFYTQYHDIEKEMNAYLDFDPDVF
                     RGKTILLPCDDPEWSNFTLYFAQNFARLGLKKLISTSFAHSSKPLNIPYQPSLFESED
                     SQFNALISPLKGKIFTLDKDLNNDGKIDHHDLAWKYLKSDGDFRSDEIKALRDEADII
                     ITNPPFSLFREFITWIMEADKKFAIIGNMNAITYKEVFPLIKDDKIWLGKGFTRSCAH
                     FFSPYESDSEYLELDRFEKLGIAVPKGLFVTRVASIHWFTNIEHGRRHQPLQLMTMTD
                     NLKFNKKLDGKNAYQQYDNYNAIEVPETKAIPSDFDGVMGVPISFLDKYCPEQFEILG
                     ATQRGCHDEVPDTKKYDDYWEMKQNGEKTGSGGGKTNENANLATNDGKK"
BASE COUNT          423 a          204 c          231 g          347 t
ORIGIN      
        1 taatttaacc tctttattcc aactcgccaa ctcttttttt aacttgggag gttggaatgt
       61 cgaataataa tgctaaaaat agcaatcttt ctaacgctaa aaaagccaaa agtgacgaat
      121 tttacacaca atatcacgac attgagaaag agatgaatgc ttatcttgat tttgatcctg
      181 atgtatttcg tggcaaaacc atcttgttgc cttgcgatga tcctgaatgg agtaatttta
      241 cactttattt tgcacaaaat tttgccagac ttggtttaaa aaaattaatt agtaccagct
      301 ttgctcatag tagcaaacct ctcaatatcc cctaccaacc aagcttattt gaaagtgaag
      361 attcacaatt taatgcccta atttcaccac ttaaaggcaa gatttttacg cttgacaaag
      421 atctgaacaa tgacggcaaa atcgatcacc acgatttagc gtggaaatat ttaaaaagcg
      481 atggcgattt tcgctctgat gaaattaaag ccctacgaga tgaagccgac atcattatta
      541 ctaatccgcc attttctcta tttcgtgaat ttattacgtg gattatggaa gcggataaga
      601 aatttgcgat tattggcaat atgaatgcga ttacttataa agaagttttc ccattgatta
      661 aagatgacaa aatttggtta gggaaaggat ttactagaag ttgtgcacat ttttttagtc
      721 cgtatgaaag tgatagcgaa tatctagagt tagatagatt tgaaaaatta ggcatagctg
      781 ttccgaaagg attatttgtt actcgagtag caagcattca ctggttcacc aatatagaac
      841 acggtcgccg tcatcaacca ttacaattaa tgacaatgac ggataatctc aaattcaata
      901 aaaaactaga cggtaaaaat gcctatcaac aatacgataa ttacaatgca attgaagtac
      961 ctgaaaccaa agcaataccg agtgattttg acggagttat gggtgtgccg atctcgttct
     1021 tggataagta ttgcccagag cagtttgaga ttttgggagc aacgcaacgt ggttgccacg
     1081 atgaagtgcc tgacacaaag aaatacgatg attattggga aatgaaacag aatggagaaa
     1141 aaacaggatc aggcggaggg aaaacgaatg aaaatgccaa tttagcgaca aatgatggaa
     1201 aaaaa
//
LOCUS       DANKBV010000250         1201 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.251, whole genome shotgun sequence.
ACCESSION   DANKBV010000250 DANKBV010000000
VERSION     DANKBV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.251"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            439..>1201
                     /locus_tag="PWY43_002508"
     CDS             439..>1201
                     /locus_tag="PWY43_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDL2137328.1"
                     /translation="MNLVDYSTSLWSVLPALIALILAIVTRKVIISLSIGILFGAFML
                     TNSNFLDALTYIKNSVVSLVYKGAEEGLNSNNVNIILFLLLLGVLTSLLSISGSNQAF
                     ANWAQKKVKGKRGAKLMAAGLVFFTFIDDYFHSLAVGAIARPVTDKFRVSRAKLAYIL
                     DSTAAPMCVLMPISSWGAYIITLIAGLLATHSITGYSPLGAFMTMSAMNFYAIFAMIM
                     VFFVAYFSFDIGSMARFERQAAHLAELDDEQIETKG"
BASE COUNT          337 a          191 c          242 g          431 t
ORIGIN      
        1 attttactct cctaaattaa tgttttatat aagtagcttt tatgctcctt ttatcatatc
       61 tcaaaaggca ttttaggtaa ataagcataa ataaaaaaat agatacttta tatctaaaga
      121 tattcgtgct atcttaaaca ctgtcctaca aattgtagag gtgcgaattc aataagtatt
      181 tcttctgagt ggaaaacgat gaaggggaag gaaaggtgaa tttgccgaaa tcaattaagc
      241 gtcatcttaa ttggttgggg tcgttgtcga aagaaacgac actgtcgtag taaatgctac
      301 ggagtgctac tgttagggtg ggtttcagat atttcacatt tgccttcctt tcatatttat
      361 ttttataaat atcccagtag atctcttgtt tggctattct tatttataca aattatacac
      421 agtcatttta gaggttttat gaatctagtt gattattcta cgtcactttg gtcggtattg
      481 ccggcactga ttgcgctaat attggcaatt gtgacacgca aggtgattat ttcacttagc
      541 atcggtattc tctttggtgc atttatgctt actaatagca attttcttga tgcacttact
      601 tatattaaaa atagcgttgt ttctttagtt tataaagggg ctgaagaagg attaaattca
      661 aataatgtta acattatttt atttttgctt ttattagggg tattaacgtc attattaagt
      721 atttcaggta gtaatcaggc gtttgcgaat tgggcgcaga aaaaggtaaa aggcaaacga
      781 ggcgcaaaat taatggcggc aggtttggtt ttctttacct ttattgatga ctatttccac
      841 agtttagcag taggtgcgat tgcccgccct gttacggata aattccgtgt ttctcgtgct
      901 aaattagctt atattttaga ttctaccgcc gctccaatgt gcgtgttaat gccaatttca
      961 agctgggggg cttatattat taccttaata gctggcttat tggctacgca ttccatcaca
     1021 ggctattcgc cactaggcgc atttatgaca atgagcgcaa tgaactttta cgccattttt
     1081 gcgatgatta tggtgttctt tgtggcatat ttctcatttg atattggttc gatggcgcgt
     1141 tttgaacgtc aagcagcgca tttagcagaa ttagatgatg agcaaattga aacgaaagga
     1201 c
//
LOCUS       DANKBV010000251         1065 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.252, whole genome shotgun sequence.
ACCESSION   DANKBV010000251 DANKBV010000000
VERSION     DANKBV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.252"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(266..781)
                     /locus_tag="PWY43_002509"
     CDS             complement(266..781)
                     /locus_tag="PWY43_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HDL2137329.1"
                     /translation="MDYLLQLLNKLELNTIVGLGTCLFTGYLAFFAWSQLKELRKQHK
                     QKSTIELLISNNSNEYYRKRRKAYMAMRDSGVNLTVLACALNDKNATAEQKRQNFIVL
                     DVLNAIEFICVGIKEDLFDEAVYKRMSKSSVIKDWNTLKPYIMELRRINNNNNKLFCE
                     FEWLATKWESE"
BASE COUNT          305 a          217 c          186 g          357 t
ORIGIN      
        1 attttgtacc tttcgttttt agttaatgcc acttagtagg tggcgggctt caactaccaa
       61 acgaaagatc ggcggagctt attccccttt cgggtgttct attaggctct ctcgacccgc
      121 catagtttta gggcatttcc taaattttgg gtacaaaaaa accgctgttt tgtcggagcg
      181 gataaccgac tttcgtttag tagtgcggtt atcttaatcc gaagtttggc ggttgtcaac
      241 aaaaaagcct caataatata tgaggctatt cagattccca tttcgttgca agccattcaa
      301 attcacaaaa gagcttgttg ttattgttgt tgattctgcg aagctccatt atgtagggct
      361 tgagcgtgtt ccagtcttta atgacactgc ttttgctcat tcgcttatat accgcttcat
      421 caaataagtc ttccttgata ccaacacaga taaattcaat cgcattaagc acatctaaaa
      481 caatgaaatt ctgtcttttt tgctctgctg tggcattttt atcatttaat gcacacgcta
      541 gaacagtaag gtttacaccg ctatcacgca tagccatata ggcttttctg cgtttacggt
      601 agtattcgtt gctgttattg ctgataagga gttcaatggt cgatttttgc ttatgttgct
      661 tgcgaagctc tttaagctga gaccacgcaa aaaaggctaa gtacccagta aataaacaag
      721 tacctagccc gactattgtg ttcagctcta atttatttaa aagctgtaaa aggtaatcca
      781 ttaaacctcc cagccctcat tagcaattaa tgtaaagtta gctaaatttt tcatatttca
      841 ctcctagatg atgaagatta ttgtaactta gacgaaaaaa gcctactcaa gttcccttgg
      901 taagctgttt tgtcatctaa attattacaa agaacaaaca attattgtca gcattactgt
      961 caataatatt taatgacgtt atattaatcc gacaccgtca tgatgtcaaa ccttttaacc
     1021 gctcttttta agttgtagca gttacacaac ttagccattc aaaac
//
LOCUS       DANKBV010000252         1064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.253, whole genome shotgun sequence.
ACCESSION   DANKBV010000252 DANKBV010000000
VERSION     DANKBV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.253"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..932)
                     /locus_tag="PWY43_002510"
     CDS             complement(<1..932)
                     /locus_tag="PWY43_002510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03571 family LLM class oxidoreductase"
                     /protein_id="HDL2137330.1"
                     /translation="MNTLSPIFSQVFGQNQLTLGLIAPFMGYQNHIPDNLTTIADNAR
                     LAENLGFSALWVRDVPFYDPHFGDVGQGLDPMVTLGFLSAHTQNIALGTAGLIAPLRS
                     PIHIAKMATSVDSLSHGRFLLGLSSGDRPVEYPAFGADFASRSERFRESIDLINTLTE
                     QDFPHFKGKHYADLTDNLDLLPKPKHRLPIVAIGRARQEMDWLANVPDAWIWHGVNPA
                     DTANIINTLADLRQKDTPTAFGYAQFVEVLDDKNAKAQLFNNIYLRGGAKGLADFWAK
                     EQEKGVKHLALNLKPTQRPADEVLTELAEYALTQ"
BASE COUNT          293 a          226 c          253 g          292 t
ORIGIN      
        1 aattgtgtca aagcatactc tgccaattct gtaagcactt catcagcagg acgctgagtg
       61 ggttttaaat tgagtgccaa atgtttcacg cctttttctt gttcttttgc ccaaaagtct
      121 gccaagcctt ttgcaccacc acgcagataa atgttgttaa ataactgtgc tttggcgttt
      181 ttgtcgtcca aaacttccac aaattgagca tagccaaatg ctgtgggggt gtctttttgg
      241 cgtaaatcgg caagggtgtt gatgatgttt gcggtgtcgg caggattcac accgtgccaa
      301 atccacgcat caggtacatt agcaagccag tccatttctt gccttgctcg tccaatcgcc
      361 acaatgggca agcggtgttt gggtttgggg agcaaatcca aattgtctgt taaatcggca
      421 taatgtttgc ctttgaaatg gggaaaatcc tgctcggtta aggtgttaat caaatcaatg
      481 ctttcacgaa aacgctcgct acgagaggca aaatccgccc caaatgcagg gtattccaca
      541 ggtctatcgc cagaggataa gcccaataaa aaccgtccat gactcaaact atccacactt
      601 gtcgccattt tggcaatgtg aatgggacta cgcaaagggg caatcagccc tgccgtacca
      661 agggcgatgt tttgggtgtg ggcggacaaa aaaccaagtg taaccatcgg gtcaagccct
      721 tgccccacat cgccaaaatg agggtcataa aacggcacat ctcgtaccca caatgctgaa
      781 aagcccaagt tttcagccag ccgtgcattg tcggcaatgg tggtcaaatt gtctggaatg
      841 tgattttgat agcccataaa gggggcaatt aagcccagtg ttaattgatt ttgcccaaaa
      901 acttgggaga aaatggggga aagcgtgttc attttggcgt acctgtctta tttaagataa
      961 ggtattttaa gggcttttga gtttgcttaa aatgggataa attgagaaag tgttttgcta
     1021 aattggcata aatagacgct ttcaagctga ctgaaaattt gcaa
//
LOCUS       DANKBV010000253         1008 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.254, whole genome shotgun sequence.
ACCESSION   DANKBV010000253 DANKBV010000000
VERSION     DANKBV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.254"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..>1008)
                     /locus_tag="PWY43_002511"
     CDS             complement(<1..>1008)
                     /locus_tag="PWY43_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2137331.1"
                     /translation="TDLAKLSGLAPASVTNLTKILIDNHFILERTVQTTLSRGRPSVG
                     LAVSNFFWQLLCVTVSSHKIEISLCKLNGAEIYKQDYTISAENYPRLNDFILNFITKF
                     RQLYCVNTSQILAVSISVIGKINAEKDSITQLGNKNIFCNITSKLSPLFHCPILLSEH
                     FQLWLLAESTVGSLINQDNAIFLQLDDTVNLSVLLSGSLLSKHSKMNVDNMLMPRFSH
                     LSDEIFPELDEIHRYKLVNQVTFPAIVRLIDRYLPNNLKSQEQKISWLCQKIDENQPL
                     ALAILSHITDNLAYALMNLVNIFSSEKIMLNSPLIKIKEPLFTQISEKLHKNLLQTNL
                     K"
BASE COUNT          343 a          153 c          172 g          340 t
ORIGIN      
        1 atttttaggt ttgtttgtaa caaattttta tgcaattttt ctgaaatttg cgtaaataac
       61 ggctctttta ttttgatcaa tggcgagttt agcataattt tttcactaga gaatatatta
      121 actaaattca ttaatgcata agctaaatta tcggtaatat gcgataaaat agctaatgca
      181 agcggttgat tttcatcaat tttttgacaa agccagctga ttttctgttc ctgacttttt
      241 aaattattag gcaaataacg atcaatgagt ctcacaatag ctgggaacgt cacctgattg
      301 actaatttat agcgatgaat ttcatctagt tcagggaata tttcatcact gagatggcta
      361 aatctaggca taagcatatt atcaacattc atctttgagt gtttagacag taaactacca
      421 ctaagcaata cacttagatt cacagtatca tctaattgta gaaaaatagc attatcttgg
      481 ttaattaatg agccaaccgt agattctgca agcaaccaca gctgaaaatg ttcacttaat
      541 aaaataggac aatgaaacag tggagagagt ttagatgtaa tgttacagaa tatatttttg
      601 ttccccaatt gagtaatact gtctttttct gcattaattt taccgatcac actaatagat
      661 acagccagga tctgactagt attgacacaa tatagttggc ggaactttgt aataaagtta
      721 agaataaaat catttagcct aggataattt tctgctgaga ttgtataatc ttgcttataa
      781 atttctgcac cattgagttt acaaagtgaa atttcaattt tatgagatga aacagtaaca
      841 caaagtagtt gccaaaaaaa attagaaaca gctaatccaa cggaggggcg acctctggat
      901 aaagtagtct gaaccgtacg ttctaaaatg aagtggttat ctattagtat tttagtcaga
      961 tttgttactg aagctggggc taaccccgat agtttagcta aatctgtt
//
LOCUS       DANKBV010000254          964 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.255, whole genome shotgun sequence.
ACCESSION   DANKBV010000254 DANKBV010000000
VERSION     DANKBV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.255"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..>964)
                     /locus_tag="PWY43_002512"
     CDS             complement(<1..>964)
                     /locus_tag="PWY43_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137332.1"
                     /translation="LNNEKSNNNDYYHFRNLINGLEDIIQILNSSELSLFNGKVLVLT
                     GQAGSGKSHLLGDFVQKRQNSGKYSLLLLGQDFTEKSNAWNQILSKQLNLNITPEVFL
                     SALDSIGEMQKERVILAIDALNEGEGKSLWNNQLNGLIELVSQYPNIGLVLSVRDSYQ
                     DIIFNKLNIKNQDKLCYIKHTGFIDNEFEAIQIFFPYYGLQLPSIPLLNPEFSNPLYL
                     KLFCESLKYQGETCVPKGHKGFNHIVKSYLDGIEKRICEQIEQDIGSHLVQRAANVIA
                     KLQLEEDKYSLDYMTVKRAICDELIVDIAETDAKKFLDYLIKENV"
BASE COUNT          326 a          167 c          119 g          352 t
ORIGIN      
        1 taacattttc tttgattaaa taatcaagaa attttttggc atctgtttct gcaatatcta
       61 ctattaattc atcacaaatt gctctcttaa ccgtcatata atccaaacta tatttatctt
      121 cttccaattg taatttggca ataacattag ctgctctttg aaccagatga gaaccaatat
      181 cctgttcaat ttgttcacaa atgcgttttt caataccatc taaataagat tttacaatgt
      241 gattaaatcc cttatgacct tttggaacac aggtttcccc ttgatacttc aaagattcac
      301 aaaataattt taaatataat gggttggaaa attctggatt aagcaaagga atgcttggta
      361 attgcaagcc ataataagga aaaaatattt gaatggcttc aaattcatta tctataaacc
      421 ctgtgtgctt gatataacac aatttatcct gatttttaat atttaattta ttaaaaataa
      481 tatcttggta gctatctcgc acactcagaa ccagtccaat atttggatat tgacttacca
      541 actcaattaa tccattaagt tggttattcc ataatgattt cccttcaccc tcatttaagg
      601 cgtctatggc taaaataacc cgttcttttt gcatttctcc gatactatct aatgcggata
      661 aaaatacttc gggtgtgata ttaagattta attgtttgga taagatttga ttccaagcat
      721 tggatttttc tgtaaaatcc tgccccaata acaacaaaga atactttcca gaattttgtc
      781 ttttttggac aaaatcaccc aataaatgag attttccact accagcttga ccagttaaaa
      841 caagcacttt accattaaat aatgataatt cagatgaatt taaaatctga ataatgtctt
      901 ccaaaccatt aattaaattt ctaaaatgat aatagtcatt attattggat ttttcgttgt
      961 ttag
//
LOCUS       DANKBV010000255          934 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.256, whole genome shotgun sequence.
ACCESSION   DANKBV010000255 DANKBV010000000
VERSION     DANKBV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.256"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..846
                     /locus_tag="PWY43_002513"
     CDS             <1..846
                     /locus_tag="PWY43_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2137333.1"
                     /translation="FGLVAKRYRDEVIPQKKASTAQTDLCKIKNLLLFFNDAPLEEIT
                     PQHIREYMTWRKDTPGSANNEFSMFSHIWQYAREWGYTNLPSPNIGMKRYPIRKREIY
                     VENNIFQMVYEVADQDMRDLMDLAYITGQRPVDLVNIRKEHIFDGYLHIVQQKTQAKL
                     RIEIVGKLADIIQDRLEKATGTHLFSTKKGEKLTARKLGKRFTYIREQAVKNNPEYAN
                     EIMAFQFRDLRAKSGTDKALSLGEEAARQQLGHTSVQMTKTYIRKAPIVTPIKESVPS
                     SKHQS"
BASE COUNT          336 a          188 c          178 g          232 t
ORIGIN      
        1 ttcgggcttg tagccaaacg ttatcgagat gaagttatac ctcagaaaaa agcgagcaca
       61 gcacaaacag atttatgcaa gataaaaaat ctactgctat tctttaatga cgctcctctc
      121 gaagaaatca cccctcaaca tatccgagag tatatgactt ggcgaaaaga cacaccggga
      181 tctgctaata acgaattttc aatgttcagt catatttggc aatacgctcg agagtggggt
      241 tatacaaact taccaagccc taatatcgga atgaaacgct accctatcag aaagcgtgaa
      301 atttacgttg agaacaatat atttcagatg gtatatgaag tggcagatca ggatatgaga
      361 gacttaatgg atctcgccta tatcaccgga caacgtcctg tcgatttggt caatatccgg
      421 aaagaacaca tttttgatgg ttatctccat attgttcagc aaaaaacaca agccaaacta
      481 cgtattgaga tagtgggaaa actggctgat attatccaag ataggctgga gaaagctact
      541 ggaacacact tattcagcac taagaaaggg gaaaagctaa ccgctagaaa actaggtaaa
      601 aggttcactt atattcggga acaagcggtc aagaataacc cggaatacgc aaatgaaata
      661 atggcattcc aatttcggga tctaagagct aaatcaggaa cagataaagc cctttctcta
      721 ggtgaagaag ccgctcgcca acaactaggt catactagcg ttcaaatgac caaaacatac
      781 attcgaaaag ccccaatagt aacccctatc aaagaaagtg ttccctcctc aaaacaccag
      841 tcttaaaaga cagtttcgga acattttgca ttttttcgga acgtttacat aaaaatcact
      901 tataatagat tgaaagataa gtaatttttg ttac
//
LOCUS       DANKBV010000256          909 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.257, whole genome shotgun sequence.
ACCESSION   DANKBV010000256 DANKBV010000000
VERSION     DANKBV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.257"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..738
                     /locus_tag="PWY43_002514"
     CDS             <1..738
                     /locus_tag="PWY43_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDL2137334.1"
                     /translation="LIVATVSMMMKTGADALAESGASFSILGAFENTTVGISLVVGGV
                     AGLIVATICIITSGNISFKNYVRAYGLGVKMMSGAITILFFAWTINGVVSDMQTGKYL
                     SGLVAESIGAEFLPAILFVLAAAMAFSTGTSWGTFGIMLPIGASMAVHSDPALIIPCL
                     SAVMAGAVCGDHCSPVSDTTILSSTGAQCNHMDHVISQLPYALLVATASIVGYLVVGF
                     TQSAVLGFIATAIVMAILILLFKEKKH"
BASE COUNT          246 a          149 c          193 g          321 t
ORIGIN      
        1 cttattgttg ctacagtctc aatgatgatg aaaacaggtg cagatgcact ggccgaatca
       61 ggtgcttcat tttctatact aggtgctttt gaaaatacta cggtaggcat ttcattagtt
      121 gttggtggtg tggcagggct tatagttgca actatttgta tcattacaag cggtaatatt
      181 tcttttaaaa attacgtccg tgcttatggc ttaggtgtga agatgatgtc tggtgcaatc
      241 actattctct tctttgcgtg gacaataaac ggcgtagtaa gtgatatgca aacagggaaa
      301 tatctatcag gattggttgc agaaagcatt ggagcagagt ttttacctgc aattctattt
      361 gtgttagccg cagcaatggc gttttcaaca ggaactagct ggggaacatt tggtattatg
      421 ttgccaattg gagcctcaat ggcagtgcat tcagatccag cattaattat tccttgttta
      481 tctgcagtaa tggctggtgc ggtttgtggt gatcactgtt ctccagtatc tgataccact
      541 attctttctt caacaggcgc acaatgtaat catatggatc atgtcatttc tcaattacct
      601 tatgctttat tagttgcaac agcttctatt gttggttact tagtggttgg ctttacccaa
      661 tcagcggtat tgggatttat tgctacagct attgttatgg caatactcat tttgcttttt
      721 aaagaaaaaa agcattaatt tccgaaaatg tgaagggaat cacagaatga gatttattga
      781 tgagataaaa tagcactcaa gatatggata tcgaatgttg tgttatataa atcttaaaat
      841 aagatcctcc tagcattagc gtaccttttg gtacgctttt ttcttttatg gcaatattat
      901 ttcttgcaa
//
LOCUS       DANKBV010000257          891 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.258, whole genome shotgun sequence.
ACCESSION   DANKBV010000257 DANKBV010000000
VERSION     DANKBV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.258"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            296..565
                     /locus_tag="PWY43_002515"
     CDS             296..565
                     /locus_tag="PWY43_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137335.1"
                     /translation="MKNKNFEIAKVVILNEQLGSPKDLELKVVENSLSDEDRYDIKQA
                     VLKAVGNGCSDPEMLASRCCSAIEIINRNGLNTGNGSISTSSNSV"
     gene            complement(543..815)
                     /locus_tag="PWY43_002516"
     CDS             complement(543..815)
                     /locus_tag="PWY43_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas2"
                     /protein_id="HDL2137336.1"
                     /translation="MRNYLISYDLYKAGQNYDGLISYIKSHQVWAAIHKSVWYIKSNK
                     SAEQIRNELLIYLDKNDSVFVTDMNSAAWNNLTEGNGEYIRQNWMR"
BASE COUNT          308 a          148 c          175 g          260 t
ORIGIN      
        1 accgctttaa gcctaatggt gaaaagtagg aagacgaaag tcggaggcag cccttaaaac
       61 gcccactact tcaaagcgtt atgccctttg gtgcaagcaa aggaatacaa gcatataccc
      121 ttatagcggt ttaggtgaaa gtctgggcgt gtacattgag acacaaggga gtaaccgaaa
      181 ttcaaattgt gcaaacgcag ctctaaaatt gcgtgacagc tcggagagac ggcatttcaa
      241 attccaaagc atatttgaga aagtgtgttt tggaatattt aactagaggt aaattatgaa
      301 aaacaaaaat tttgaaattg cgaaagttgt tattttaaat gaacaactag gaagccctaa
      361 agatttggaa ttaaaagtag tagagaatag cctatctgat gaagatagat acgacatcaa
      421 acaggcggta cttaaggctg tagggaatgg ttgttcagat ccagaaatgc ttgcatcacg
      481 atgttgttcc gcaatcgaaa taattaatag aaatgggcta aacactggta atgggagtat
      541 atctacctca tccaattctg tctaatgtat tcaccatttc cttcagtcag attattccaa
      601 gcagcactat tcatatccgt aacaaagaca ctatcgttct tatctaaata aatgagcagt
      661 tcattacgaa tttgttctgc agatttatta gatttgatat accaaactga tttatgaata
      721 gcagcccaaa cttgatggct tttaatataa ctaatcaatc catcgtaatt ttgcccagct
      781 ttgtataaat cgtaagaaat taaatagttt ctcatagaag aatccttatt tgtgctgtga
      841 gagatttaat tatattcctt agtgttgtga gagacaataa ggaaacttga c
//
LOCUS       DANKBV010000258          868 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.259, whole genome shotgun sequence.
ACCESSION   DANKBV010000258 DANKBV010000000
VERSION     DANKBV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.259"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..672)
                     /locus_tag="PWY43_002517"
     rRNA            complement(<1..672)
                     /locus_tag="PWY43_002517"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          209 a          231 c          141 g          287 t
ORIGIN      
        1 tctatacctt gcaactacac gcccagttaa gactcggttt ccctacggct cccttattca
       61 gttaaccttg ctacaaaata taagtcgctg acccattata caaaaggtac gcagtcacaa
      121 gattactctt gctcccactg cttgtacgca caaggtttca ggttctattt cactcccctc
      181 gccggggttc ttttcgcctt tccttcacag tactggttca ctatcggtca atcaggagta
      241 tttagccttg gaggatggtc cccccatctt caaacaggat atcacgtgtc ccgccctact
      301 tgttgttagc ctagtaccac aatggagttt taaggtacgg gattatcacc ctctacgatt
      361 gtccttccca gaacattccc ctaactttac tgctatcact aactggctct ttcgcgttcg
      421 ctcgccgcta ctaacgaaat ctcggttgat ttcttttcct cggggtactt agatgtttca
      481 gttctcccgg tttgcctcac acagctatgt attcactggg tgatagtagg ttcttcacct
      541 actgggtttc cccattcgga catcttggat taaacgcctc ttatcgactc atccaagctt
      601 ttcgcagatt agcacgtcct tcttcgcctc tgattgccaa ggcatccacc ttgtgcgctt
      661 agtcacttaa ctatacaacc tcaaatgttt tctaaaactt ctcaaaaata aaagtaaaaa
      721 aacaatgaag tcagttttac aactaaacac ttgactgctt ttgttcagtc aagatttttt
      781 ctactcagac tttcaattta ttcgttatca cttatgtata attacttatg cgtaacgaac
      841 ttgaaatatc tctcagtttt tcagcttg
//
LOCUS       DANKBV010000259          844 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.260, whole genome shotgun sequence.
ACCESSION   DANKBV010000259 DANKBV010000000
VERSION     DANKBV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.260"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(28..213)
                     /locus_tag="PWY43_002518"
                     /pseudo
     CDS             complement(28..213)
                     /locus_tag="PWY43_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          310 a          163 c           98 g          273 t
ORIGIN      
        1 ccaccatact ttctccatta aagtatttca acttacttta tacattactt taattgcatc
       61 ttcagaaagt aaatcacaac gaggtaatac ttcattgata gccttccaag aaagtaaatc
      121 ttgctactct atagcacctt tcattaaaag tggttgtttc tcagaataac tttcacgaaa
      181 ttcttgataa gaaattggaa attgaatttt cattttcatc tccactagcc ttgataatct
      241 gtaccatcac cacaatcgta gtttgagcca gattggttat tattcgttct acctggaact
      301 ccaaacgtag gctttattgg taaagatgat ggaattaatt cttttacacc gctaggtaaa
      361 gaattttcat gtattgtaat aggtaggctc ctcgctaaat caagggctaa attaacagaa
      421 gtactggata aaccgcctaa agcccccctc ttgcaaaccc tgtagcaaaa gcaacgccat
      481 aagatacaag atctggttga ttagccttat tttcagaatt ccctccaact actaagctta
      541 attgtaaaat tgatggttct ttcatttcat tctcccttta atctaattct gatcacaaaa
      601 accaatatta aaaataattc taaaaataat actatactta caacatcttc cacaatatat
      661 aaaacattaa gttccttcat atatccatct tttataaaaa agaaaaaatt agacagcatc
      721 gaaaaaaaca aaaccttcca tatagttttt ttcataaaaa aataaagact attatttgct
      781 gacatacaaa tatccttata tacaaaagga agcaaaatac ttgatgtaaa aaacaatact
      841 ttaa
//
LOCUS       DANKBV010000260          835 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.261, whole genome shotgun sequence.
ACCESSION   DANKBV010000260 DANKBV010000000
VERSION     DANKBV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.261"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..421)
                     /locus_tag="PWY43_002519"
     CDS             complement(<1..421)
                     /locus_tag="PWY43_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2137337.1"
                     /translation="MLIEWRNQIVGDKIKAVTWNNYVRHLKALYNFGISQGLLNINRN
                     PFNQLFIRESKRRKKTYSTEQLHKIDHILNNNVVLPDFLKPSWFVLAIIMTLRCTGIR
                     RGQLVKLKIGDVDLSNRTIYISPEINKNHDYHIVPISD"
     gene            complement(577..>835)
                     /locus_tag="PWY43_002520"
     CDS             complement(577..>835)
                     /locus_tag="PWY43_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HDL2137338.1"
                     /translation="QETGQPYDKETINNLQYDFQGLGIHSPKNTMTNGKPDTINFWKC
                     DVIGPRKTSSLTGYLIKNTRLFFGDKVLLNNHCLTLQKEET"
BASE COUNT          252 a          139 c          144 g          300 t
ORIGIN      
        1 aatctgaaat cggtacgata tggtaatcat gatttttatt gatttccggc gaaatataaa
       61 tcgtcctatt agaaagatct acatcaccaa ttttcagttt cactaattgt cctcgccgaa
      121 tccccgtaca tctcaatgtc ataataattg caaggacaaa ccagcttggt tttagaaagt
      181 caggtaatac aacgttatta ttgagtatgt ggtctatttt atgtaattgt tcggttgaat
      241 aagttttctt tctccttttc gattctcgaa taaataactg attaaatgga tttcgattta
      301 tattaagtaa tccttgcgat ataccaaagt tatataaggc ttttaaatgt ctgacataat
      361 tattccacgt tactgctttg attttatcgc caacaatttg attacgccat tcaatgagca
      421 tatcggtggt aatgtcattg gcatttaact ttggataacg ctttttgata attcggatta
      481 cgttgttgta gctccgtttg gtatccggac gaagatagtg acgatagaaa aaaaaatcgg
      541 tctagtagcg aatcaaaaga gatagaagtt atcattttaa gtttcctctt tctgtaaagt
      601 taaacagtga ttatttaata gtaccttatc cccaaaaaag agacgggtat tttttatcaa
      661 atatcctgtt aaagaggaag tctttctcgg tccaatgaca tcacatttcc aaaaattgat
      721 ggtatccggc ttaccgttgg tcattgtgtt tttaggtgaa tgaatcccta atccttgaaa
      781 atcatactgg agattattaa ttgtttcctt atcataaggc tgtccagtct cttgc
//
LOCUS       DANKBV010000261          832 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.262, whole genome shotgun sequence.
ACCESSION   DANKBV010000261 DANKBV010000000
VERSION     DANKBV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.262"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            31..>832
                     /locus_tag="PWY43_002521"
     CDS             31..>832
                     /locus_tag="PWY43_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2137339.1"
                     /translation="MWVRLGDICLKITDGTHHSPPNIDKGDFLYITAKNIKKDGLDLS
                     KISYVTKEIHNEIFSRCNPEKGDILYIKDGATTGVSIINTLNEPFSMLSSVALIKTSQ
                     EIDNEYLNYVMNSHYFYNISIGSMSGTGIPRITLTKLESYLVPVPPLLEQQRIVQKIE
                     ELLPLVERYEQTEQQLTKLNNTFPEQLKKSVLHAAIQGKLTEQDPNDELASCLIERIK
                     AEKNRLIAEKKLKKPKSVSEIVMRDNLPYEIKAGQERCIADEVPFEIPQ"
BASE COUNT          302 a          141 c          157 g          232 t
ORIGIN      
        1 gaaccacctt ttgagattcc tgagagttgg gtgtgggtga ggttggggga tatatgccta
       61 aaaattacag atggaactca tcattctccc ccaaatatcg ataagggtga ttttctttat
      121 attacagcta aaaatataaa gaaagatgga ttagatttat caaaaattag ctatgtaaca
      181 aaagaaattc ataatgaaat tttcagccgt tgtaatcctg aaaagggaga tattctatat
      241 attaaagatg gagccacaac tggagtaagt attataaata ctcttaatga gccatttagt
      301 atgttatcca gtgttgcctt aattaagacc tcacaagaaa tagataatga gtacttaaat
      361 tatgtaatga atagtcatta tttttataac atttctattg gaagtatgtc tggaacaggt
      421 attccaagaa taacattaac taaattagaa tcttatttag tcccagttcc gccactccta
      481 gaacaacagc gcattgtgca aaaaatcgag gaattattac cgcttgttga acgctacgag
      541 caaaccgaac agcaactcac taaactcaac aatacattcc ccgaacagct gaaaaaatcg
      601 gtgttacacg cagctattca gggtaaatta accgagcaag atccgaatga tgaactggca
      661 tcttgcctga ttgagcggat aaaagcggag aaaaatcgtc ttattgccga gaaaaaactc
      721 aaaaaaccaa aaagtgtttc agagattgta atgcgtgata atttacctta tgagatcaaa
      781 gcaggtcagg aacggtgtat cgccgatgaa gtaccatttg agattcctca aa
//
LOCUS       DANKBV010000262          831 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.263, whole genome shotgun sequence.
ACCESSION   DANKBV010000262 DANKBV010000000
VERSION     DANKBV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.263"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            141..>831
                     /locus_tag="PWY43_002522"
     CDS             141..>831
                     /locus_tag="PWY43_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL2137340.1"
                     /translation="MLNWAGKSTAYQTLQTPTSKTLTPCEEESVDFENTQNIFIEGEN
                     LDVLKALQKSYFNSVKMIYIDPPYNTGNDFIYNDSFADTKADYAEKVGDVDEHGKLKR
                     AFVRNSKENGHYHSNWLNMMLPRLHLAKNLLRDDGVIFISIDDNEQAQLKLLCDEVFG
                     EENFVAILSVENNPKGRKNSKFISVSNDFCIIYAKNKEMGKFVENIPKNAKDMCQDEN
                     GHYVHASGKRVL"
BASE COUNT          286 a          142 c          161 g          242 t
ORIGIN      
        1 ttgccaactt ttcgcttaaa gatcaacaac tttctcaatt aaaatccctt ttacctgaaa
       61 ttttcagcga aggtaaaatc gacttcgaca aattccaaca acttttttcg gataattttt
      121 ccaccgaacc tgaccgctat atgctcaact gggcaggaaa atcaacagcc tatcaaacct
      181 tacaaacccc aaccagcaaa acgctcactc cttgcgagga agagagtgtg gattttgaaa
      241 atacgcagaa tatctttatt gagggtgaaa atttagatgt attaaaagcc ctgcaaaaat
      301 cctacttcaa ttcggtcaaa atgatttata tcgacccgcc atacaataca ggcaacgatt
      361 ttatttataa cgacagtttt gccgatacca aagcagatta tgcggagaaa gtgggcgacg
      421 ttgatgaaca cggcaaattg aaacgagcct ttgttcgtaa cagcaaagaa aatgggcatt
      481 atcacagtaa ctggctgaat atgatgttgc ctcgtttgca tctcgcaaaa aatttactgc
      541 gtgatgacgg ggtgattttt atttcaattg atgataacga acaggcacag ctgaaattgc
      601 tttgtgatga agtgtttggg gaggagaatt ttgttgccat attatcagtt gaaaataatc
      661 cgaaaggtag aaagaattct aaatttattt ctgtaagtaa tgatttttgt attatttatg
      721 ctaaaaataa agaaatgggg aaatttgttg aaaatattcc taaaaatgct aaggatatgt
      781 gtcaagatga aaatggacat tatgttcacg caagtggaaa acgagtatta g
//
LOCUS       DANKBV010000263          809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.264, whole genome shotgun sequence.
ACCESSION   DANKBV010000263 DANKBV010000000
VERSION     DANKBV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.264"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            296..643
                     /locus_tag="PWY43_002523"
     CDS             296..643
                     /locus_tag="PWY43_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137341.1"
                     /translation="MFREEAIEYQSKKWKSTAVLFSRVPALVVFSISFFIFTAFILFI
                     VFGNYTRRETVVGELVMQSHPILLSAPKSGYISENYIQPHQQVKKRAIRWLKLRWIEL
                     RIAKILMLILFFR"
BASE COUNT          273 a          104 c          135 g          297 t
ORIGIN      
        1 tgaataatat atcttgtctg tatagatgta agattaatta atgtatgtaa ctaaatttta
       61 ttgcgagtgt tttatgagat ttgttactgt aatttatatt gcgtttattt attttatgat
      121 ttacagattc gtttatccat tattaccatt cgagtgtaat acagaagttt atgatatagt
      181 gttgttttgc tttttgttta cttattctct tcttaagaag taacatgttt ataagattag
      241 cttttaatgt atataaaata aatgtgctaa tgtagtagtg taaaattggt attatatgtt
      301 cagagaagaa gctattgaat atcaatcgaa aaaatggaaa agcacagctg tactatttag
      361 cagagttcca gctttggttg tcttttccat ttctttcttt atctttactg cctttattct
      421 atttattgtg tttggtaatt atacccgccg tgaaacggtt gtgggtgaat tagtgatgca
      481 atctcatccg attttattat ctgccccaaa atccggctat atatccgaaa attatattca
      541 accacatcag caggtaaaaa aaagggcgat ccgctggtta aaattacgct ggatagaatt
      601 acgcatagcg aaaatattaa tgttaattct attctttcgt tgaaatcaca aattcaagca
      661 acagaacaag cgataactgc tttatataag aataaatcag aaactattaa tagccttgaa
      721 aagcagatca aaaacagtca aaaaatttat caagataagc agagttattt agtggaaatt
      781 gaaaaaagta tgaatgacta tgcggagtt
//
LOCUS       DANKBV010000264          804 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.265, whole genome shotgun sequence.
ACCESSION   DANKBV010000264 DANKBV010000000
VERSION     DANKBV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.265"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..437)
                     /locus_tag="PWY43_002524"
     CDS             complement(<1..437)
                     /locus_tag="PWY43_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL2137342.1"
                     /translation="MTTLTFQNTTLSVIDQNNQKWMPALEVGRALGYKNPSSDISKLY
                     ERNKDEFTPSMTAIIDMDTASGMQKVRIFSLRGCWLLGMRSHTKVAKDFRKWVLDILD
                     KEVLQNNQQIAPLAEPTITAEEQNMLQNAVKATHERTKLSYGE"
BASE COUNT          203 a          164 c          179 g          258 t
ORIGIN      
        1 atttcaccgt agctgagctt ggtgcgttcg tgcgtggctt tcaccgcatt ttgtagcata
       61 ttctgttctt cggcagtgat tgttggctct gcaagcggtg cgatttgttg attattttgc
      121 aaaacttctt tatctaaaat atccagcacc catttgcgga agtctttggc gactttggtg
      181 tggctacgca tacctaatag ccagcaacca cgcaagctga aaattctgac tttctgcata
      241 ccgctagcgg tatccatatc aatgatggct gtcattgatg gagtaaattc atctttgttg
      301 cgttcgtaca gcttagatat atcagacgat ggatttttat aacctaacgc tctgccaact
      361 tctaaggcgg gcatccattt ttgattattc tgatcgataa cggaaagtgt agtgttttgg
      421 aaagttaatg ttgtcattct gaatttctca tatgtaagtt taaaaactca tcacgagtat
      481 tgcagtactg gtgatgaact aggcaaggtc tgcaataacc gtccatacga gacaacggca
      541 gatctttcga tctccttacc tagcccatca ttgacaacat aggggatttg caaattgcaa
      601 gcgcctagtt ttagggggta cttccaaatt ggaagtgcct tatttagggg tactgtcaaa
      661 ttgacagtgc cttgatttga ctagattccg gctataaaaa aagtcgcatt gagcgactat
      721 cattcttcac cgctcgtatg gtattcagga attgcagttc ccgactttac tgttgaaagt
      781 gcggttatct tagccgaaag ggcg
//
LOCUS       DANKBV010000265          769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.266, whole genome shotgun sequence.
ACCESSION   DANKBV010000265 DANKBV010000000
VERSION     DANKBV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.266"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..606
                     /locus_tag="PWY43_002525"
     CDS             <1..606
                     /locus_tag="PWY43_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137343.1"
                     /translation="AISANLGKVTAGTITGTTISGGTISGTTITGGIIKGARIEGLTG
                     KFTGELEINQLIGGNIYEAKTWTSKATGKSKTYSETKNSFALSDTYYEYVCQFVLPAT
                     KATRTLYVVHTPTGESKSKTFYGSGSNRTPTTITLPSVIDISNTISPNVSNPFNDSGA
                     ILIANTQYVVTCRAWCAGRGRSAKVNFRAIISSTNGSSYQL"
     gene            616..>769
                     /locus_tag="PWY43_002526"
     CDS             616..>769
                     /locus_tag="PWY43_002526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137344.1"
                     /translation="MKKIILLTTIMVSACTTQYKQDYTTKPTFCYQLAPQEQLPGKNC
                     IGSGGHS"
BASE COUNT          278 a          127 c          156 g          208 t
ORIGIN      
        1 gcaatatcgg caaatcttgg taaggtaact gctggtacaa ttacaggtac gacaattagt
       61 ggtgggacaa tttcaggtac aacaattaca gggggaataa ttaaaggagc aagaattgag
      121 ggattaacag gtaaatttac tggagaactt gaaatcaacc aattgatcgg aggtaatatc
      181 tatgaggcta aaacttggac atcaaaagca acagggaaaa gtaaaacata ttcagaaacg
      241 aaaaacagtt ttgcattaag tgatacatat tatgagtatg tatgtcaatt tgtactacct
      301 gctacaaaag cgacgagaac attgtatgtt gtccacactc cgacaggaga gagtaaaagc
      361 aaaacttttt atggtagtgg tagtaatcgg acaccgacaa caattacatt accgtctgta
      421 attgatatta gcaatactat ttcacctaat gtatctaacc catttaatga ttctggggca
      481 atactcattg ctaatactca atatgtagta acgtgtcgtg cgtggtgtgc agggcgtggg
      541 agaagtgcta aagtgaattt tcgagcaatt atatcatcta caaatggttc atcttatcaa
      601 ttataggagg caaatatgaa aaaaattata ctacttacaa caattatggt ttcagcttgt
      661 acaactcaat ataagcaaga ctatacaaca aaaccaacct tttgttatca actagcccct
      721 caagagcaat taccggggaa aaactgtatt ggctcaggag ggcattcag
//
LOCUS       DANKBV010000266          746 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.267, whole genome shotgun sequence.
ACCESSION   DANKBV010000266 DANKBV010000000
VERSION     DANKBV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.267"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            79..666
                     /locus_tag="PWY43_002527"
     CDS             79..666
                     /locus_tag="PWY43_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HDL2137345.1"
                     /translation="MFDELKIAQATAYLLHKAGGTMEHIKVMKLLYLADRLSWQRYGY
                     SISNDEYYSLPYGPVLSNTLNLMRGEEASLYQKTSIWGEWIADKEDHKISLKKSVNTE
                     DEYFFDCLSESDTEILEEVFATFGNYKPFALVEYTHNPKYIPEWEDPQGRSKPISLNT
                     LLTKLGKTPSEIESILEQQTEKGEMDNLFKGLINA"
BASE COUNT          271 a          141 c          149 g          185 t
ORIGIN      
        1 gtatttgaac aaatcgcacg tctttaaaat attaaattaa gagaaagcac tatttgcgat
       61 gtagcaggag ataaaacaat gtttgatgaa ttaaaaatag cccaagcaac cgcctatttg
      121 ctacataagg caggcggaac aatggagcat attaaggtaa tgaaattgct ttatcttgcg
      181 gatcgcttat cgtggcaaag atacggctat tctatttcta atgatgaata ttattcattg
      241 ccttatggac cggtattaag caacacactc aacctaatgc gaggagaaga agcgagctta
      301 taccaaaaaa ccagtatttg gggcgagtgg atagcagata aggaagatca caaaatcagc
      361 cttaaaaaat ccgtaaatac cgaagatgaa tacttctttg attgcctttc agaaagcgac
      421 accgaaattt tagaagaggt atttgcgacc tttgggaatt ataagccatt cgccctagtg
      481 gaatataccc ataaccctaa atacattccc gaatgggaag acccacaagg cagaagcaag
      541 ccgatttctt taaacacgct tcttactaag ctagggaaaa cgccaagcga gattgaaagc
      601 attttagagc aacagacaga aaaaggagaa atggataacc ttttcaaggg cttaatcaat
      661 gcttgaagtc aaaagacaag caactttctt agaccgacca actacggcaa ggtagagaac
      721 tcctacaagc atttatatat tctgct
//
LOCUS       DANKBV010000267          744 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.268, whole genome shotgun sequence.
ACCESSION   DANKBV010000267 DANKBV010000000
VERSION     DANKBV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.268"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..204)
                     /locus_tag="PWY43_002528"
     CDS             complement(<1..204)
                     /locus_tag="PWY43_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HDL2137346.1"
                     /translation="MAPRKKKAIELPLLPLRDVVVFPYMVMPLFVGREKSIQALRSAM
                     DSNKQLFLVTQQDPNKEEPNAEDM"
     regulatory      408..526
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT          226 a          149 c          143 g          226 t
ORIGIN      
        1 catatcttct gcattcggct cttctttatt tggatcttgc tgagtgacta aaaacagttg
       61 tttattggaa tccatcgccg agcgtagggc ttgaattgat ttttcacgtc ctacaaataa
      121 cggcataacc atataaggaa aaaccaccac atcacgcaat gggagtagtg gtaattcaat
      181 cgctttcttt tttcttggtg ccataaattt gctctctcta tcaaaatata atcccccatt
      241 atgagggcga atttgggaaa aacaagggaa aatttgattt atcttaaaaa aactcgaata
      301 gtcaaaacag tagatgttgg cgatttaatc aattgttaaa atcagggtaa ttagcttaat
      361 ttcccacttg acatcaaatc gtaatgcgag caaaatcccc tgcatatttt aatcttcagg
      421 gcagggtgaa attcccgatc ggcggtaaag tccgcgagcc gagcaatcgg caggatttgg
      481 tgaaattcca aaaccgacag tatagtctgg atgaaagaag agtcaaaaac cgcaaaaaat
      541 accgcacaag cggtcatttt ttgtgtcttt tttgcaagcc ttgagtcgtg agatttcaag
      601 gcttcattgt tttgcaggcg gcgtttgcaa attttttact aaaaacgacc gcttgtattg
      661 caataatcct ttcattacgc cctcaattta tcaaaatgca tatgacagat gttcaatata
      721 tggcttatgc catcaccctt gccg
//
LOCUS       DANKBV010000268          691 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.269, whole genome shotgun sequence.
ACCESSION   DANKBV010000268 DANKBV010000000
VERSION     DANKBV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.269"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            131..>691
                     /locus_tag="PWY43_002529"
     CDS             131..>691
                     /locus_tag="PWY43_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025298382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2137347.1"
                     /translation="MKLIEIENLNKYFGLGQTQAHILKNINLSIERGDFVAIIGASGS
                     GKSTLMNIIGCLDTASSGICRIDGKETQTMSADELSDLRQRKLGFIFQRYNLLSALNA
                     NENVALPAIYAGMDSSSRKKRADTLLTKLGLSDKTQHKPNELSGGQQQRVSIARALMN
                     GGEIILADEPTGALDSKSGETVLEILR"
BASE COUNT          233 a          134 c          139 g          185 t
ORIGIN      
        1 caccaaggta ctattcattt agataaaagt aatatgggag gattaaaaat cgaactgaaa
       61 cttccccttt ggcgttccta aggcaaaaag catatcgttt gtaaaaaaca gtcgaaaaat
      121 aaccgcttgt atgaaattaa ttgaaataga aaatctcaat aaatactttg gcttgggaca
      181 aacccaagcc catattctaa aaaatatcaa tctcagtatt gaacggggtg attttgtcgc
      241 aattataggt gcttcaggtt ctggcaaatc taccttaatg aacattattg gctgtttaga
      301 tactgcaagc tcaggtattt gccgtattga tggcaaagag acccaaacaa tgtctgcaga
      361 tgaactgtct gatttacgcc aacgtaaact ggggtttatt ttccaacgct ataatctcct
      421 ttcagctttg aatgcaaacg aaaatgttgc cctacctgca atttacgccg gaatggatag
      481 ctcatcacgc aaaaaacgag ctgatacgtt attaacaaag ttaggattat cagataaaac
      541 ccaacataag ccgaatgagt tatctggggg gcaacagcaa cgagtcagca ttgcacgagc
      601 cttaatgaac ggcggtgaaa tcattcttgc agacgagcct accggtgcat tagattccaa
      661 aagtggcgaa actgtattgg aaatcttgcg a
//
LOCUS       DANKBV010000269          687 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.270, whole genome shotgun sequence.
ACCESSION   DANKBV010000269 DANKBV010000000
VERSION     DANKBV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.270"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..650
                     /locus_tag="PWY43_002530"
     CDS             <1..650
                     /locus_tag="PWY43_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2137348.1"
                     /translation="AEFSNSQNANVQKWSAFVKGSVIRQDFLACALNWVSKGNISDYM
                     SRHRFDENIAELKTYFTTVIDWVNTTFFDAKKEMCGLEWGRLYENYGKQAYDLSKINA
                     EIDRFYDDPCVKNHKGIWEYVLGGMTDKRLLDIRIFDDKTKKDTYSKQTEKAKVANHS
                     NCPLCAVSDNNNKTKIWTLKEMEADHVSAWSKGGLTGAENCEMLCISHNRAKGNK"
BASE COUNT          247 a          101 c          151 g          188 t
ORIGIN      
        1 aagcagaatt tagcaattct caaaatgcca atgtgcaaaa atggtcggca tttgtgaaag
       61 gttcagttat tcgccaagat tttttggctt gtgcgttaaa ttgggtaagc aagggaaata
      121 tcagtgatta tatgagtcgc catcgttttg atgagaatat cgctgaattg aaaacttact
      181 ttactaccgt gattgattgg gtaaatacca cattctttga tgctaaaaaa gagatgtgtg
      241 gtttagaatg gggcagactt tatgaaaatt atggcaagca agcttatgat ttaagcaaaa
      301 ttaatgctga aattgaccgc ttttatgatg acccttgtgt gaaaaaccat aaaggtattt
      361 gggaatatgt attgggcgga atgacagata aacgtttgct agatattcgg atttttgacg
      421 acaaaacaaa aaaagatacc tatagcaagc aaaccgaaaa agccaaagta gccaatcact
      481 ctaactgccc attgtgtgct gtgagcgata ataacaataa aaccaaaatt tggacattaa
      541 aagagatgga agccgaccac gttagtgcct ggagtaaagg agggttaact ggagcagaaa
      601 actgcgaaat gttgtgtatc agccataatc gagcaaaagg aaacaaatga taattttaca
      661 aacggttagt ttcaacgaaa atttgca
//
LOCUS       DANKBV010000270          684 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.271, whole genome shotgun sequence.
ACCESSION   DANKBV010000270 DANKBV010000000
VERSION     DANKBV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.271"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..188
                     /locus_tag="PWY43_002531"
     CDS             <1..188
                     /locus_tag="PWY43_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL2137349.1"
                     /translation="NLGRIRHDRVQSANLTLWKRDWHWLGLMPKLQFSYAKHRSNLPS
                     LYAYTDKNINLIIESRF"
     gene            complement(438..>684)
                     /locus_tag="PWY43_002532"
     CDS             complement(438..>684)
                     /locus_tag="PWY43_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HDL2137350.1"
                     /translation="ETRLRNEGKTKEEIAQHPEYLQLKRDLNDKQQTFDNAYGTGSKT
                     KRAVDAVTAALQGLAAKDSGQAAVGLASPYLNEQIKK"
BASE COUNT          177 a          164 c          135 g          208 t
ORIGIN      
        1 ttaatcttgg cagaatccgc catgaccgtg tgcaaagtgc taacctcacg ctttggaaac
       61 gagattggca ctggctcggc ctgatgccga agctgcaatt tagttacgcc aaacaccgta
      121 gcaacctgcc gagcctctat gcctacaccg ataaaaacat caatttaatt attgaaagtc
      181 ggttctagct ccataaaaac aacaagcggt taaaattcct agaaattttg caaattgcaa
      241 aaaatgtaga aaatttaacc gcttgtatct atactgcagc ttcttttcct tcagtataaa
      301 ttaatataca ccgttgcaac aataatggct gctccctgtc atcaccttga actgaaaatt
      361 gctcaaggtg gatatgacta tcgcagccgc ttccaccgca cccagtaatg tatgggcaag
      421 caggttggct tgggtatcta ctttttaatt tgttcgttga gatatggcga agctaagcct
      481 actgctgctt gtcctgaatc tttcgccgct aaaccttgta aggctgccgt caccgcatcc
      541 accgcacgct tggttttgct gcccgtgccg taggcgttat caaaagtctg ctgtttatcg
      601 tttaaatccc gttttaattg gagatattca ggatgttgag cgatttcttc ttttgttttt
      661 ccctcatttc tcaaacgggt ttcc
//
LOCUS       DANKBV010000271          674 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.272, whole genome shotgun sequence.
ACCESSION   DANKBV010000271 DANKBV010000000
VERSION     DANKBV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.272"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            70..648
                     /locus_tag="PWY43_002533"
     CDS             70..648
                     /locus_tag="PWY43_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137351.1"
                     /translation="MIEFDQIDDSFYHFPFRPRPGDTSSDTATPCIGGVDLVSNPEKV
                     ELIPEAAHSPMLKKLLTDLNTPNSKLLTLGCAYWAHKDNRDTSYAYLEFSFREHSVAT
                     NLEFIRSIDEQFEQFLQENKKQLSIEFSVPEQAFDIVSQALFWSIRPFSYFGSEERIL
                     IYFQAGSPRHQDLEIFLDLLHRFLTEYLVVPA"
BASE COUNT          209 a          139 c          123 g          203 t
ORIGIN      
        1 aaactgagcc ttggttaaaa attctgctag aatactgttc cccaacaaat aaactagcag
       61 aggttaaaaa tgattgaatt tgatcaaatt gacgatagtt tctatcactt cccatttaga
      121 ccacgtccgg gcgacacttc aagcgacact gccacacctt gtattggtgg agtagatctt
      181 gtttccaatc ctgaaaaggt agaactaata ccagaagccg cacactcccc aatgttaaaa
      241 aagttactca cagacttgaa cactccaaac agcaaacttc taacactagg ctgtgcttat
      301 tgggctcata aagacaatag agatacatca tacgcttact tagagttttc attccgggaa
      361 cattcagttg caactaacct agaatttatc agaagtattg atgaacagtt cgagcaattc
      421 ttgcaagaaa ataaaaagca gttatctatt gaattttccg tgcctgagca agcatttgat
      481 atagtttcac aagctctttt ctggagtatt cgtccatttt cttacttcgg aagcgaagag
      541 cgcatcttga tatactttca ggctggtagt cctcgacatc aggatttaga aatctttttg
      601 gatttactac accgcttttt gactgagtat ttagttgttc cagcataaca tttactcgca
      661 tttggaaagg gtgg
//
LOCUS       DANKBV010000272          671 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.273, whole genome shotgun sequence.
ACCESSION   DANKBV010000272 DANKBV010000000
VERSION     DANKBV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.273"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>671
                     /locus_tag="PWY43_002534"
     CDS             <1..>671
                     /locus_tag="PWY43_002534"
                     /inference="COORDINATES: protein motif:HMM:NF023638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137352.1"
                     /translation="DANRFTVMNSKEQTITNNDSWLCATYFEDNQWQIQELSTFANSA
                     GVAATHLHSFCPVNRSQFAFGFHNGDASPREIGFVKCSINWATGRVTTDKIILSETLS
                     RAAAEPCLRYYKNKLFLTTRSQNPNVGSLLIRCDLNGENISSFNISTEKIHYTNMPFH
                     IENDVIYMFAAERAENEWETGQSDKSGNRYRANRPRMFLLKHRLSDWGKPEKTEVQVI
                     SQGIYS"
BASE COUNT          209 a          132 c          148 g          182 t
ORIGIN      
        1 gacgcaaacc gttttacggt gatgaatagc aaagaacaga ctattaccaa caacgatagc
       61 tggctttgtg caacctactt tgaagataac caatggcaaa ttcaagaatt aagcacgttt
      121 gccaatagtg ctggcgttgc cgctacgcac ttgcatagtt tttgcccagt gaatcgttca
      181 cagttcgcct tcgggtttca taatggtgat gcttcacctc gtgaaatcgg ctttgtgaaa
      241 tgtagcatca actgggcaac aggtagagtg acaacggata agattattct ttctgaaacg
      301 ttaagtcggg ctgccgctga gccttgtttg cgatattaca aaaacaaatt atttttaacg
      361 acccggtcac aaaatccgaa tgtagggtca cttctaattc gttgtgactt aaacggggaa
      421 aatatcagtt cattcaatat ttccacggaa aagattcact ataccaatat gccatttcat
      481 attgagaatg atgtgattta tatgttcgcc gccgaacgag cggaaaatga atgggaaaca
      541 gggcaaagcg ataaaagtgg caaccgctat cgagcaaatc gtccacgaat gtttttgctt
      601 aagcatagat taagcgattg gggcaaaccg gaaaaaacag aagttcaggt tatttcgcaa
      661 ggcatttatt c
//
LOCUS       DANKBV010000273          642 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.274, whole genome shotgun sequence.
ACCESSION   DANKBV010000273 DANKBV010000000
VERSION     DANKBV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.274"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..234)
                     /gene="secG"
                     /locus_tag="PWY43_002535"
     CDS             complement(<1..234)
                     /gene="secG"
                     /locus_tag="PWY43_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL2137353.1"
                     /translation="MLINVLTIVYLVVAVLLIAFVLMQQGKGADAGASFGAGASATVF
                     GSAGSANFLSRTTALLATIFFAISLVIGNLSSHN"
     gene            complement(340..>642)
                     /locus_tag="PWY43_002536"
     CDS             complement(340..>642)
                     /locus_tag="PWY43_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252007.1"
                     /note="decatenates replicating daughter chromosomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2137354.1"
                     /translation="GFLIRKGKNIHSTEAGRILIQALPDSATQPDMTAHWEMQLNQIS
                     KKETSYQQFMFDLNQQLPHLLQSPNRQVLQNLAKITPLASGNKPRYFKKRTQTAEN"
BASE COUNT          179 a          116 c          134 g          213 t
ORIGIN      
        1 attgtgtgag cttaaattac cgataactaa actaattgcg aagaaaatcg ttgcaagtag
       61 agcagttgtt cttgataaaa aatttgctga tcctgctgag ccgaaaactg ttgctgaagc
      121 acccgccccg aaagaagcac ctgcatcagc gcctttacct tgttgcatta atacaaatgc
      181 gatcagtaat acggcaacta caagataaac aatagtgagt acattaatta acattaattt
      241 attctcttgt attctttatg cgaaatttgc aatggcgtgc ataatataga atttacgccc
      301 gtttgacaag tgctaaacag taatttttac ctgcttgatt taattttcag cagtttgtgt
      361 tcgcttttta aaataacgag gtttattgcc gcttgcaagc ggtgtgattt tcgctaaatt
      421 ttgcaacact tggcggtttg ggctttgtaa taaatgtggg agctgttggt ttagatcaaa
      481 cataaattgc tgatagctgg tctctttttt gctgatttga ttcagctgca tttcccaatg
      541 agcggtcata tccggctggg tagcagagtc gggaagtgct tgaatcaata ttctgccggc
      601 ttcggtactg tggatatttt tccctttacg gattaaaaaa cc
//
LOCUS       DANKBV010000274          612 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.275, whole genome shotgun sequence.
ACCESSION   DANKBV010000274 DANKBV010000000
VERSION     DANKBV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.275"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..222)
                     /locus_tag="PWY43_002537"
     CDS             complement(<1..222)
                     /locus_tag="PWY43_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137355.1"
                     /translation="MIQNDPRDAIILSLLANAVYGWHNNSQISYQETEEVNLDARLRQ
                     VEGDVREIKSNYLTKEAFYKAGGSTLIALV"
     gene            complement(227..607)
                     /locus_tag="PWY43_002538"
     CDS             complement(227..607)
                     /locus_tag="PWY43_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137356.1"
                     /translation="MNNKRIKPEQFADFITQRTGRNLTCPICNSQEHYLHDGYDTFTE
                     LCGEKIIGVPTIPYRIEPSPDEVMKFAAPEFHSLHFNEREGTKYWLDQKARTAVIVTC
                     VCCNNMLFFDRERILEWIKGSQNE"
BASE COUNT          180 a          121 c          100 g          211 t
ORIGIN      
        1 tactaatgca atgagtgtag agccaccagc tttataaaaa gcctctttcg tcaaataatt
       61 tgacttgatt tctcttacat cgccttcaac ttgtcttaat cgagcatcta aattcacttc
      121 ttcagtctcc tgataactaa tttgtgaatt attatgccag ccgtatactg catttgcaag
      181 taaggatagt atgattgcat ctctaggatc attttgaatc attggtctat tcattttgtg
      241 agcccttaat ccattctaaa attctctctc tgtcgaaaaa aagcatatta ttacagcaaa
      301 cacaagtgac aataacggct gttctcgctt tttggtctaa ccaatatttt gttccttccc
      361 gttcattgaa atgcaaagaa tgaaattcag gagctgcaaa tttcataact tcatcaggag
      421 atggctctat tcggtatgga atggttggaa caccgataat tttctctccg cataattccg
      481 taaaagtgtc atagccatcg tgtaaataat gttcttggct attacaaata gggcaagtta
      541 aattccgccc agtacgttgc gtgataaaat ccgcaaactg ttcaggttta atgcgtttat
      601 tattcatttt aa
//
LOCUS       DANKBV010000275          609 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.276, whole genome shotgun sequence.
ACCESSION   DANKBV010000275 DANKBV010000000
VERSION     DANKBV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.276"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            102..>609
                     /gene="rlmE"
                     /locus_tag="PWY43_002539"
     CDS             102..>609
                     /gene="rlmE"
                     /locus_tag="PWY43_002539"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDL2137357.1"
                     /translation="MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQT
                     DRLFKPGMTVVDLGAAPGGWSQYVVTQIGSKGRVIACDILDMNPIVGVDFLQGDFREE
                     SVLNALLERVGEDKVNVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLATKGSF
                     VVKVFQGEG"
BASE COUNT          184 a           99 c          155 g          171 t
ORIGIN      
        1 taattaagca taataagccg agctaaattt tagaacatct cttgaatttg ctaatttaac
       61 ccttattata aatcgttgaa ttaattaaga tagaagaaca aatgggtaga aaacgaagtg
      121 cgagttcatc acgttggctt gcagaacatt ttaaagatca atttgtgcaa aaagcacata
      181 aacaaaaatt gcgttcacgg gcgtatttta agttagatga aattcaacaa accgaccgtt
      241 tattcaaacc gggaatgacg gtggtggatc tcggtgccgc accgggcggt tggtcgcaat
      301 atgtggtgac gcaaatcggc agtaagggca gagtaatcgc ttgtgatatt ttagatatga
      361 acccgattgt aggcgtggat ttcttacaag gcgacttccg tgaagaatcg gtgctgaatg
      421 cgttacttga gcgagtaggc gaagacaaag tgaatgtggt gatgtcggat atggctccca
      481 atttcagcgg tatgccgtcg gttgatattc ctcgtgcaat gtatttagtg gaattggcat
      541 tggatatgtg tcgccaagta ttagcaacca aaggcagttt tgtggttaag gtattccaag
      601 gcgaaggat
//
LOCUS       DANKBV010000276          594 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.277, whole genome shotgun sequence.
ACCESSION   DANKBV010000276 DANKBV010000000
VERSION     DANKBV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.277"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..101
                     /locus_tag="PWY43_002540"
     CDS             <1..101
                     /locus_tag="PWY43_002540"
                     /inference="COORDINATES: protein motif:HMM:NF016343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL2137358.1"
                     /translation="KPGGFLGIQPVYGVTHWQPLPEPPKEKANERN"
     gene            312..536
                     /locus_tag="PWY43_002541"
                     /pseudo
     CDS             312..536
                     /locus_tag="PWY43_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
BASE COUNT          212 a          109 c          120 g          153 t
ORIGIN      
        1 tcaagccggg tggattctta ggtattcagc ctgtctatgg tgttacccac tggcaacctc
       61 ttcccgagcc accaaaagag aaagcaaatg aaagaaatta aactaggtgt atcgccactc
      121 acaaatgaaa tttatgcagg ctatgtcaat aaaaaaggcg atttatgaac tgaaaagcaa
      181 gatgtgacaa tgccggctct attatgtgta gttcaacact gtttgaagtt tggctcccca
      241 atcattatct caaaagaaaa tggcgagcct gaatttgaaa ttactgtaaa ggatttaaga
      301 aaatgagtaa agaaaacaac ggctggattt ccattgagga caaattacca cctattgaaa
      361 cggatgtgct agggttatgc gatatttcag gaatgcagtt aattttaata gtttcaagag
      421 aacttgccga taacgagtgg tattttctta gtgtaaatca atatgggctt gatgacgatg
      481 tgattgcagt aacccactgg caaccacttc caaagctgcc aaaggacaac caatgacaga
      541 caccaaacga ttagataaac taaacattaa aggtttccta aatgaaaaac aaac
//
LOCUS       DANKBV010000277          576 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.278, whole genome shotgun sequence.
ACCESSION   DANKBV010000277 DANKBV010000000
VERSION     DANKBV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.278"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..539
                     /locus_tag="PWY43_002542"
     CDS             <1..539
                     /locus_tag="PWY43_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2137359.1"
                     /translation="QDTFYVGNLKGVGRIYQQTFIDTYSKVAFAKLYTMKTAISAADM
                     LNDKVLPYFESQGLPMLRILTDRGSEYCGKVENHDYELYLAINDIEHSKTKVKHPQTN
                     GICERFHKTILQEFYQVAFRKKIYTDLTTLQADLDEWLMYYNHHRTHQGKMCCGRTPM
                     ATLLDGKRIWVEKNLSSN"
BASE COUNT          207 a           95 c          122 g          152 t
ORIGIN      
        1 cacaagatac tttctatgtg ggcaacctca aaggagtggg tcgtatttat caacaaacgt
       61 ttattgatac ttacagtaaa gtggcgtttg caaaacttta tacaatgaag accgctatca
      121 gtgctgcgga tatgctgaat gataaagtgt taccatactt tgaaagccaa ggtttaccga
      181 tgttacgcat attaactgac cgaggaagtg aatattgtgg caaggtagaa aatcacgatt
      241 atgagcttta tttagcaata aatgatattg aacacagtaa aaccaaggta aaacatccgc
      301 agactaacgg catctgcgaa cggttccata aaacaatctt acaagaattt taccaagtgg
      361 catttaggaa gaaaatttat acggatttaa cgacattaca agcggattta gatgagtggt
      421 taatgtatta taatcaccat cgaacacatc aaggaaaaat gtgctgtggc agaacaccga
      481 tggcaacctt acttgatgga aaacggattt gggtggaaaa gaatttaagc tcaaattaat
      541 ctgacagaca cggtaattta aaacggggga ctgtca
//
LOCUS       DANKBV010000278          576 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.279, whole genome shotgun sequence.
ACCESSION   DANKBV010000278 DANKBV010000000
VERSION     DANKBV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.279"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            116..364
                     /locus_tag="PWY43_002543"
     CDS             116..364
                     /locus_tag="PWY43_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137360.1"
                     /translation="MISGEKMTWESLGASAIGGAIGGGIGATMKAGAVTASTLSALGG
                     GMGEGLYKSTSQGIKNSIGIKASCNQSGSDYGDGCDYQ"
     gene            376..>576
                     /locus_tag="PWY43_002544"
     CDS             376..>576
                     /locus_tag="PWY43_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137361.1"
                     /translation="MKIQFPISYQEFRENYFEKKPLLMKGAISQKDLLSWKSINEILP
                     RCDLISEDAIKVMHKGKRAHKKD"
BASE COUNT          185 a           67 c          147 g          177 t
ORIGIN      
        1 gaggaatatc tcttatgaaa gagttaaaaa ttgaggattg ccaatttgtt tcggcaggct
       61 tgcacttgtt attgtaggag ctgttgctgg agctgcatat tatgttggga atagtatgat
      121 ttctggggaa aaaatgactt gggagagttt gggagcatca gctataggtg gggctattgg
      181 tggcggtatt ggagcaacaa tgaaagctgg tgcggtgaca gcgtcaacct tatctgcttt
      241 aggtggtggt atgggggagg gcttgtataa aagtacttct caaggtatca aaaatagtat
      301 tggtataaag gcgagttgca atcaatcagg ttcggattat ggtgatggat gtgattatca
      361 ataagagagt atattatgaa aattcaattt ccaatttctt atcaagaatt tcgtgaaaac
      421 tattttgaga aaaaaccact tttaatgaaa ggagcaatat ctcagaaaga tttactttct
      481 tggaagtcaa taaatgaaat attacctcgc tgtgatttaa tttctgagga tgcaattaag
      541 gtaatgcata aagggaaaag ggctcataaa aaagat
//
LOCUS       DANKBV010000279          573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.280, whole genome shotgun sequence.
ACCESSION   DANKBV010000279 DANKBV010000000
VERSION     DANKBV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.280"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>573
                     /locus_tag="PWY43_002545"
     CDS             <1..>573
                     /locus_tag="PWY43_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL2137362.1"
                     /translation="HTTPLNPPTGEPAPAEVVLNYLNSALATLAAQLGERKPVGYSLK
                     PWVKNIAARIRESSVADCCQVVDYLVAKWGRDEKMREYLCPKTIFRQSNFADYLPKSK
                     AWADNGKPVCVNGKWVAPAELEKRLIMPTVEEVRALFQKSLSGNPFKALDFTNKRNLV
                     MYHATINTKNKRPLERDLPMIISQEIKNAVE"
BASE COUNT          162 a          131 c          143 g          137 t
ORIGIN      
        1 catactaccc cccttaatcc cccaacgggg gaacctgccc cggctgaagt tgtattgaat
       61 tatctcaatt cggctttggc aacgctggcg gcgcaacttg gcgagcgtaa gcctgtgggt
      121 tactcactca agccttgggt gaaaaacatc gctgcacgca ttcgggaaag ttcggtggcg
      181 gactgttgcc aagtggtgga ctaccttgtc gctaaatggg gacgagatga aaaaatgcgt
      241 gaatatctct gccctaaaac gatttttcgc caatcgaatt ttgcggatta tctgcctaaa
      301 tcaaaagctt gggcggataa cggcaagcct gtttgcgtaa atggtaagtg ggttgcacca
      361 gcagagcttg agaaacgctt gattatgcca acggtagaag aagtacgagc attgttccag
      421 aaatcattga gtggcaatcc gtttaaagcg ttggacttta ccaacaaacg caatttagtg
      481 atgtaccacg ccactatcaa caccaaaaac aagcgaccac ttgagcgaga tttgccgatg
      541 attatcagcc aagagattaa aaatgccgtt gag
//
LOCUS       DANKBV010000280          570 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.281, whole genome shotgun sequence.
ACCESSION   DANKBV010000280 DANKBV010000000
VERSION     DANKBV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.281"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(129..>570)
                     /locus_tag="PWY43_002546"
     CDS             complement(129..>570)
                     /locus_tag="PWY43_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137363.1"
                     /translation="VVYSTDDENISIIFKRSNFMHLCGVKYDDGAREFFYAAINNRLD
                     INKIKTKSDGTTFLKLSLLKSINFLLSSEITLTNNAIYLNIAFDKALKTKKQVFALTL
                     IDNNRSFIPQSLLNLKNINNFPNGKVIKSIKSVHLQSRTEIIYL"
BASE COUNT          198 a           72 c           90 g          210 t
ORIGIN      
        1 tgttagggca taaggatatc agcgtaacac tgggatatat tgagcacgat gtggaaatgt
       61 tgagggagac agtgaattta ctctgataaa agaaaaatgg gagaatataa tttctcccat
      121 tcttttttct ataaatagat aatttctgtt ctactttgta gatgaacaga tttgatactt
      181 ttaattactt ttccattagg gaagttattt atattcttta gattaagtaa tgactgaggt
      241 atgaatgagc gattattatc gattagagtt aatgcaaata cctgcttctt agtttttaat
      301 gctttatcaa atgcaatgtt tagataaata gcattatttg tcaaagttat ttcagagctt
      361 agtaaaaaat taattgattt taacaatgac aattttagga atgttgttcc atcagattta
      421 gtcttaattt tgtttatgtc taatctatta ttaattgcag cataaaaaaa ctctcgtgct
      481 ccatcatcat atttaacacc acataaatgc ataaaatttg aacgcttaaa aataatagaa
      541 atattctcgt catcagtaga atacacgact
//
LOCUS       DANKBV010000281          563 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.282, whole genome shotgun sequence.
ACCESSION   DANKBV010000281 DANKBV010000000
VERSION     DANKBV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.282"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            141..>563
                     /locus_tag="PWY43_002547"
     CDS             141..>563
                     /locus_tag="PWY43_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL2137364.1"
                     /translation="MDKYTTLGVNILDAIATDDFELLEKLFKQGEKSYLETSPKEGWN
                     WLHKSLLGFESDEPSGKMVEYLISKGITVNAQDCYGMTPLHYAMRAKNVDAAIALLNA
                     GADPNIPNQDNLIPLRMCIRYPERLDLLELMLKKGGDVH"
BASE COUNT          209 a           68 c          122 g          164 t
ORIGIN      
        1 attatgttaa tgcaagaccg gagaggttca gattggaaaa tatgtctgaa aatcgtagcc
       61 ataagaatga gatgccgggt aaggataata ttgaacctat tattcgtgat atgaaaaaat
      121 ttgaaaaagg aaataagtaa atggataaat acactacatt aggagttaat attctggatg
      181 caatcgctac agatgatttt gagttattgg agaaattatt caaacaaggg gaaaaaagct
      241 atttagaaac atcacctaaa gagggttgga attggctaca taaatcttta cttggatttg
      301 aatctgatga accttccgga aaaatggttg agtatttaat tagtaaagga attactgtca
      361 atgcccaaga ttgctacgga atgacccctt tacattatgc aatgagggca aagaatgttg
      421 atgcagcgat agcattatta aatgcggggg cagatccgaa tattccaaat caagataatt
      481 taataccatt gagaatgtgt attcgttatc ctgaaagact agatttattg gaattgatgt
      541 taaaaaaagg tggagatgtt cat
//
LOCUS       DANKBV010000282          560 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.283, whole genome shotgun sequence.
ACCESSION   DANKBV010000282 DANKBV010000000
VERSION     DANKBV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.283"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            49..>560
                     /locus_tag="PWY43_002548"
     CDS             49..>560
                     /locus_tag="PWY43_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL2137365.1"
                     /translation="MLNYLNSALATLAVQLGERKPVGYSLKPWAKNIAARIRESSVAD
                     CCQVVDYLVAKWGRDGKMREYLCPKTIFRQSNFADYLPKSTAWASNGKPVCVNGKWVA
                     TAELEKRLIMPTVEEVKVLFQKSLSGNPFKSLDFSNKRNLVMYHATINTKNKRPLERD
                     LPMVISQEIK"
BASE COUNT          157 a          125 c          147 g          131 t
ORIGIN      
        1 acttcccccc ttaatccccc aacgggggag cctgctcctg cagaagttgt gttgaattat
       61 ctcaattcgg ctttggcaac attggcggta cagcttggcg agcgtaagcc tgtggggtat
      121 tcgctcaagc cgtgggcaaa aaacattgct gcacggattc gggaaagttc ggtggcggac
      181 tgttgccaag tggtggatta ccttgtcgcc aaatgggggc gagacggcaa aatgcgtgaa
      241 tacctctgcc cgaaaacgat tttccgccaa tcgaattttg cggattattt gccaaaatcg
      301 accgcttggg cgagcaatgg caagccggtt tgcgtaaatg gcaagtgggt agcaacagca
      361 gagctagaaa aacgcttgat tatgccaacg gtggaggaag ttaaagtctt gtttcagaaa
      421 tcactaagtg gcaatccgtt taagtcgttg gatttcagca ataaacgcaa tttagtgatg
      481 taccacgcca ccataaacac caaaaacaag cgaccacttg agcgagattt gccgatggtt
      541 atcagccaag aaattaaaaa
//
LOCUS       DANKBV010000283          543 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.284, whole genome shotgun sequence.
ACCESSION   DANKBV010000283 DANKBV010000000
VERSION     DANKBV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.284"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            97..>543
                     /locus_tag="PWY43_002549"
     CDS             97..>543
                     /locus_tag="PWY43_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialyltransferase"
                     /protein_id="HDL2137366.1"
                     /translation="MIKTIKKLLVSPIEFFQDSWFLKFHLAEDYRKTTNLFFISQMGQ
                     LEQYQGLIEKLKLKNNVLIVLYTKKNQLMPKNIAERCNKELFNSIRFLCLPKSPMRLN
                     IKNYIMMLNSYKLLLKRIKPKELYISSFERHYSLLGTLAKNMGFKVN"
BASE COUNT          213 a           52 c           74 g          204 t
ORIGIN      
        1 tttattttta tgtgtatttt gatattatag tctctctttc tgtaagaaat tagttcaatt
       61 tcaataagat tattaattag cgatgtaggt taaaaagtga ttaagacaat aaagaaatta
      121 ttagtatctc ctatcgaatt ctttcaggat tcgtggtttt taaaatttca tttagcagaa
      181 gattatagaa aaacaacaaa tctatttttt attagtcaaa tggggcagct agaacaatac
      241 caaggattaa tagaaaaact aaagttaaaa aataatgtat taattgtatt atatacgaaa
      301 aaaaatcaat tgatgcctaa aaatattgct gaaagatgta ataaagaatt atttaattct
      361 attaggtttt tatgtttgcc taaatctccg atgagattaa atattaaaaa ttatataatg
      421 atgcttaatt cttataagct tcttctaaaa agaattaaac caaaagaatt atatatttct
      481 agttttgaga gacattattc tttattaggg actctcgcaa aaaatatggg ttttaaggtt
      541 aat
//
LOCUS       DANKBV010000284          527 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.285, whole genome shotgun sequence.
ACCESSION   DANKBV010000284 DANKBV010000000
VERSION     DANKBV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.285"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>527
                     /locus_tag="PWY43_002550"
     CDS             <1..>527
                     /locus_tag="PWY43_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2137367.1"
                     /translation="GYLGSQDTFYVGNIKGVGRIYQQTFIDTYSKVAFAKLYTMKTAI
                     TAADMLNDKVLPFFESQRLPMLRVLTDRGSEYCGKVENHDYELYLAINDIEHTKTKVK
                     QPQTNGICERFHKTILQEFYQVAFRKRIYMDLEALQADLDEWLMYYNHHRTHQGKMCC
                     GRTPMETLVDGKRIWA"
BASE COUNT          176 a           87 c          121 g          143 t
ORIGIN      
        1 ggctatttag ggtcacaaga taccttttat gtcggcaata ttaaaggtgt gggtcgtatt
       61 tatcagcaaa cgtttattga tacttatagt aaagtggcat ttgcaaaact gtatacaatg
      121 aagacggcta tcactgctgc tgatatgcta aatgacaaag tattaccatt ctttgaaagc
      181 cagaggttac ctatgttacg agtgctgaca gaccgaggca gtgagtattg tgggaaagtt
      241 gagaatcacg attatgagct ttatttggct atcaatgaca tagagcacac aaaaaccaaa
      301 gtgaaacagc cacaaacaaa cggcatctgt gagcgttttc acaaaacgat tttgcaggag
      361 ttctaccaag tggcattcag aaaacggatt tatatggatt tagaggcatt gcaagctgac
      421 ttagatgaat ggttaatgta ttataaccat catcgaaccc atcaaggaaa aatgtgctgt
      481 ggaagaacac cgatggagac attagttgat ggaaaacgca tttgggc
//
LOCUS       DANKBV010000285          511 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.denovo.286, whole genome shotgun sequence.
ACCESSION   DANKBV010000285 DANKBV010000000
VERSION     DANKBV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.denovo.286"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..348)
                     /locus_tag="PWY43_002551"
     CDS             complement(<1..348)
                     /locus_tag="PWY43_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875262.1"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2137368.1"
                     /translation="MSEKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNS
                     VDEALSGFADKIDVILHADNSLEVVDNGRGMPVDIHSTEKISGVELILTKLHAGGKFS
                     NKNYTFSGGLHGVG"
BASE COUNT          154 a          120 c           90 g          147 t
ORIGIN      
        1 ccccaccccg tgcaaaccac cggaaaacgt atagttttta ttggaaaatt taccgccggc
       61 gtgcaatttc gttaagataa gctccacccc tgaaattttc tcggtggaat gaatatcgac
      121 cggcattccc cgcccgttat ccaccacttc caacgaatta tcagcatgaa gaatcacatc
      181 aattttatct gcaaagccgg acaacgcctc atcgacacta ttatcaatca cctcctgccc
      241 caaatggttt ggacgtgtgg tatccgtgta cattcccggg cgaagctgca cgggttcaag
      301 atcttttaga acggtaattt catccgcccc atagcgtttt tcagacatta ttctttctct
      361 taggctataa aatgaagctg aattttaagg aaaattcagc tgttttgcaa aattttggtg
      421 aaaaatgacc gcttgtcgcc aagatattga ttctctgatg aaaaaattta taattaataa
      481 aacgctagtt attcactaag gaataaaaaa t
//
LOCUS       DANKBV010000286         2422 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 37736
            SAMN05326220-rid16803703.guided.205, whole genome shotgun sequence.
ACCESSION   DANKBV010000286 DANKBV010000000
VERSION     DANKBV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326220
            Sequence Read Archive: SRR3822677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584873.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584873.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 06:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,555
            CDSs (total)                      :: 2,520
            Genes (coding)                    :: 2,441
            CDSs (with protein)               :: 2,441
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 17 of 79
            Pseudo Genes (internal stop)      :: 27 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326220-rid16803703.guided.205"
                     /isolate="37736"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..752
                     /locus_tag="PWY43_002552"
     CDS             <1..752
                     /locus_tag="PWY43_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL2137369.1"
                     /translation="AKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSI
                     SVSPNYSYRTVAYRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHL
                     QKRYFEELTASRYNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLAR
                     VGAVFQGERWATQASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPK
                     LNFRYQKIDSNIADFYPRQNKEVFFTLEKLF"
     gene            897..1370
                     /gene="gpt"
                     /locus_tag="PWY43_002553"
     CDS             897..1370
                     /gene="gpt"
                     /locus_tag="PWY43_002553"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2137370.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            1483..2106
                     /gene="gmk"
                     /locus_tag="PWY43_002554"
     CDS             1483..2106
                     /gene="gmk"
                     /locus_tag="PWY43_002554"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2137371.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGHFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            2196..>2422
                     /locus_tag="PWY43_002555"
     CDS             2196..>2422
                     /locus_tag="PWY43_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2137372.1"
                     /translation="MKLSKLAFYVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLD"
BASE COUNT          687 a          453 c          586 g          696 t
ORIGIN      
        1 cagcaaaagg cgtgcgttat ggcttagggt tgagtaaatt gcacaacgtg aaaggcaatc
       61 attttgtcgg tttaacgctc aattatagcg ggatttacta ttgggataac cgagattacc
      121 gtgagcaatc catatccgtt tcgccgaatt atagctaccg caccgtcgct tatcggctga
      181 ctttttcgcc ttttattgag caaaattggc tggggagtag ccgttataat gttcaatttg
      241 gggcaacact caacggtttg cgttacctta attcgcaatg gttggtgtcg gggagtttta
      301 cccatttgca aaaacggtat tttgaggagc taaccgcaag ccgttataac agctatcagc
      361 accaactcag tggcggtgtg cagtggcaag cggtcaaaaa tgggcggttt tttgcaaatt
      421 tagacggcgt gcgtgaaatt gcacgggaaa aagcacaaag ctcgaataag tggctggctc
      481 gagtgggggc ggtttttcaa ggcgaacgct gggcaacgca ggcaagtatc ggctttggca
      541 agcgatattt tgccgacaag cactatttgt ttggctataa gcggttagac acggaatatc
      601 aagccaattt agcggtttgg aaccgcactt ggcattggca gggcattgtg ccgaagctga
      661 atttccgtta tcaaaaaatt gacagcaata ttgctgattt ttaccctcgc caaaacaagg
      721 aagtcttttt cactttagaa aagctatttt agtgttggtt ttttgtgcta agcatacgat
      781 tgcgtagctg ccggcacaaa aaataaatga aatgatggcg aaaaaagcat tttcgggtta
      841 tcctagtgcg attttttata tcggggtagc ccccaatttt atattaggtt gtttctatga
      901 gtgaaaaata cacaaatgaa aaatatgtcg tgacgtggga tatgttccat atgcacgctc
      961 gcaagttggc agaaaattta ttacctgcca cacagtggaa agggattatt gcggtgagcc
     1021 gtggcggctt gtttccggca gccgttttag cacgtgagtt gggtattcgc cacgtagaaa
     1081 cagtgtgcat tgccagctac gatcacgata gccaaggtga gttaaatgtg ttacacgcag
     1141 ccgataccga tggcgaaggc tttattgtgg tagatgattt agtggatact ggcaacacgg
     1201 caaaagaaat tcgcagaatg tacccgaaag ctcgttttgt aaccgtgttt gcgaaaccgc
     1261 acggtgcacc gttggttgat gattatgtga ttgatattcc acaagaaacg tggattgagc
     1321 agccttggga tttaggcatt accttcgttc agcctttggc acgtagataa ttttgtgtaa
     1381 tggacaatga aagttgtcca ttatttttta tttattccta tgaaatgtag gggcgaatca
     1441 tcattcgccc tattcccaaa atcaaatagg tatatacaaa taatgagttt aggcaatctt
     1501 tatattattt ctgccccaag tggagcagga aaatcttcct taattaacgc cttgttggcg
     1561 gatttacccc gttcggaagt acagctttcg atttcacaca ctacccgtca gcctcgtccg
     1621 gcagaacagg acggcgtgca ttattatttc acttcgcacg atgagtttaa gcagctgatt
     1681 gagcaagggc attttttaga atgggcggag gtgtttggca attattacgg cacatcattg
     1741 ccgatgattg aacgtagcct agagcagggc attgatgtct ttttggatat tgactggcaa
     1801 ggggctagac aaattcggga aaaagtgccg aatgtgaaaa ccgtttttat tttgccaccg
     1861 tcaaaaacag aattggaaaa acgtttaatc gggcgtgggc aagattccgc tgaaaccatt
     1921 gccaaacgta tggcagaggc ggtgtcggaa atgaaccatt acaatgagtt cgattatgtg
     1981 attattaatg atgatttcca acaggcattg gcagagctaa aagcgatttt aattgccgag
     2041 cgtttgagct taggttcgca acaaattcgt catcagcatt tgattactga tttgttaaat
     2101 aattaaatga acagttgttc atttaactgg aaaggcaatc tgtttttggg tagaatacgc
     2161 ccattttccc ctctttattt tcaaaaggaa atgtaatgaa actttcaaaa ttagcatttt
     2221 atgtggcgtt ggctctaacc gtaacggctt gtaccacagg gaatatgagc aatgacggga
     2281 ctttgcaaca agcacagcaa acctatcaaa gctatgatga aattaccaaa caatatcaaa
     2341 ttaacgagca atggtggcaa ggctatacgg atgcggaatt aaaccgtgtg gttgatttag
     2401 ccatcaaaaa taaccttgat tt
//
